LOCUS       DAOSOW010000001        19634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_2767_length_19634_cov_4.048317, whole genome shotgun sequence.
ACCESSION   DAOSOW010000001 DAOSOW010000000
VERSION     DAOSOW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 19634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19634
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2767_length_19634_cov_4.048317"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1586)
                     /locus_tag="PLD67_00005"
     CDS             complement(<1..1586)
                     /locus_tag="PLD67_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOT20940.1"
                     /translation="MDIIKMKENHKVLTEYKYELGEIDKGYTNRTLYVNISDNTITSK
                     PITEMMKEKFVGGKGFGLWYLWNATSPETKWDSPENEIIIAGGPVCGITQYAGTGKSL
                     VCSISPLTDIPIDSNVGGFFGPLLKFSGWDALEIQGKSDKDIIVYIDGNNGLVTIEEA
                     PLEAVDAHVLGEQLTEMYADNEDDKRNVAVVTAGTGAEHSRFGILNFTFYDPRRKVTR
                     LKQAGRGGIGTVFRNKKLKALIVKYSGVKGNLNNNFDQSIINKTGIKYHKEIHDLDHL
                     QNNMRKIGTANIIEIMDAYDLLPVMNFQYGSHPDTQNIKSTVFIEKYLTQGVQDGCWY
                     GCSMACAKGADNFLIKTGPYKGHRVTVDGPEYENAAGLGANCAIFDPEAILELNFYCD
                     TYGIDTISFGTATAFAMECYERGIINKEITGGLELNFGNFYASVELLHQMSKGEGFGT
                     VVGQGVHRMKKIFVEKYGADEEFLFDIGMEQKGLEYSEYGSKESLAQQGGYGLTNKGP
                     QHDEAWLIFMDMVNNQIPTF"
     gene            complement(1596..2024)
                     /locus_tag="PLD67_00010"
     CDS             complement(1596..2024)
                     /locus_tag="PLD67_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT20941.1"
                     /translation="MKYLAKDDSKCIQCGACESICSKTYFKEDNPEKSCIQIKKEDTP
                     EINVCNQCGECIDICPVMAIKRDKFGVVRIDKKICVGCFMCVGFCPLLSMRQHDDYIE
                     PFKCIACGLCAKECPTEAIYIAERSDEEAREMPIDKVFNR"
     gene            complement(2173..3054)
                     /gene="hslO"
                     /locus_tag="PLD67_00015"
     CDS             complement(2173..3054)
                     /gene="hslO"
                     /locus_tag="PLD67_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOT20942.1"
                     /translation="MDYMIRAMDKKHTFRLFMVRATNTVEEARQHHNTTPTASAALGR
                     TLIAGLMMGYMMKNEKDRLTININGGGPIGTILVVSDNSGHVKGYVGNPNVDLDLKEN
                     GKLNVGGAVGTQGKLTVMMDIGLKEPYVGSTDLVTGEIGEDIAFYYYLSEQQNTAVAL
                     GVLVDIDYSIKAAGGFMVQTLPFIEEEDLVKLENTLNNLKSISDYFNNEKDLEEIAKE
                     IFSDFDIEVFDRIPVSFNCDCSEERMEQALISLGKNELKKIIEEDGKIETVCHFCNKK
                     YMFEGDKLKNILKYIEE"
     gene            complement(3057..3791)
                     /locus_tag="PLD67_00020"
     CDS             complement(3057..3791)
                     /locus_tag="PLD67_00020"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT20943.1"
                     /translation="MKNSYKEFSQIYDLLMDIDYEKWTSFIMKKLKGKRKILEAACGT
                     GGITKLLADNNYKVTAFDLSEDMLMRAYEKLAQSPMVRLLNQNMVDFEIDEKFEAAIC
                     CCDGINYLTKEEVKSFFQRIYHHLNEGSKFIFDMSTKYKYDKMFCDTYVYDDGDIFYV
                     WENELVNDEKINIEINFFIKDTDKYTRITEEQTQYIHTVDEIIDLLEDAGFENIEIFD
                     DYNEKEYSDTSLRAVFSACKFDNERT"
     gene            complement(3803..4003)
                     /locus_tag="PLD67_00025"
     CDS             complement(3803..4003)
                     /locus_tag="PLD67_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HOT20944.1"
                     /translation="MAEKKEKNKKNKKKKEPTPNDIMKMEIAEELGLTDKVKKFGWGG
                     LTAKETGRIGGLMTAKKKKRKK"
     gene            complement(4060..4197)
                     /locus_tag="PLD67_00030"
     CDS             complement(4060..4197)
                     /locus_tag="PLD67_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20945.1"
                     /translation="MLDFDFRVIAVVMFFIILISIQFTLKKIFLLLREIRAILLTKRD
                     R"
     gene            complement(4305..4757)
                     /locus_tag="PLD67_00035"
     CDS             complement(4305..4757)
                     /locus_tag="PLD67_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT20946.1"
                     /translation="MYDIKKILVPVDGSPASIKASERAIEIAKKYGSEITFLSVANTP
                     DMFNIGDYSFRVHYNYDNVVNNHKLAQAEILDKVIEGLDIEGVKFEKKVISGEPAEEI
                     IRMAHDCQYDYIVMGRRGFSKFTRFFVGSVTQRVIAESPCAAVIVVNE"
     gene            4971..5684
                     /locus_tag="PLD67_00040"
     CDS             4971..5684
                     /locus_tag="PLD67_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOT20947.1"
                     /translation="METKKETIIKAAGAAFPQTIPILAGYVFLSIAYGIYMNAFGFNF
                     LYPMFMSMLIYAGTVQFVAVGLLMAVFDPINAFVLTLMINARYLFYGISMLEKYRVKG
                     IRGAFLIFSMSDETFSINCTANIPEDVDKGWFMFFVTLFNYCYWVSGSILGGLFGELI
                     EFNTKGLEFAMTAMFIVIFMEQWLKEKSHSSSLLGLGISLLCLIIFGSKNFIIPSMIL
                     ILLILSLKRELFMEAGESK"
     gene            5681..6013
                     /locus_tag="PLD67_00045"
     CDS             5681..6013
                     /locus_tag="PLD67_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010344887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter permease"
                     /protein_id="HOT20948.1"
                     /translation="MTMTVGQHIITIAMVVLGTMITRFLPFIIFPADKPTPKYIQYLG
                     KALPPASLGLLVVYSLKDVNIFTGSRGIPELIAVITVLVIHLWKRQMLLSIAVGTITY
                     MLLVQFVL"
     gene            complement(6072..6153)
                     /locus_tag="PLD67_00050"
     tRNA            complement(6072..6153)
                     /locus_tag="PLD67_00050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6117..6119),aa:Leu,seq:cag)
     gene            complement(6238..6981)
                     /locus_tag="PLD67_00055"
     CDS             complement(6238..6981)
                     /locus_tag="PLD67_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOT20949.1"
                     /translation="MENKVLAIVDGREIKESDLKNLLRNLGQNAVHFQGEEGRKKLID
                     ELVMHELMYSDAVENNLENEEEFIEVFNDMKKSMLQQYNLRKMFNKVTISDEELKDFY
                     EKNKYFYNRPEMVKASHILVDTEERANEILEDITDGLSFEDAAKEYSNCPSKQDGGFL
                     GQFGKGQMVKEFEDAVFSMRAGEISPPVKTQFGYHIIKLIEHMPARSAEFEEVYQEVK
                     DNYFVIKQEEVYKSKKEELIKKYDVVLFE"
     gene            complement(7031..7297)
                     /locus_tag="PLD67_00060"
     CDS             complement(7031..7297)
                     /locus_tag="PLD67_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013431304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOT20950.1"
                     /translation="MRSQKVVLKNKIGLHARPAALFVKKARNFDSEIIIKKGIQEANG
                     KSIISIMALGAMKGDELVIITKGSDEDKCIAELTNLLEFMEEEE"
     gene            complement(7294..8262)
                     /gene="whiA"
                     /locus_tag="PLD67_00065"
     CDS             complement(7294..8262)
                     /gene="whiA"
                     /locus_tag="PLD67_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HOT20951.1"
                     /translation="MTFSSRVKDELSRTEISDVLCANAEAAAFVRTVGYITIKGLNKV
                     EFEFLTENAAVARRIFKVLKIAHNINADVSVSKSNRLKKQNNYIIKIDEKLTKQFLSD
                     SQIIKDNDFNVMNFNYEVPEELLASDLCRRAFIKGSFMGSGSISNPEKSYHAEFVSNK
                     EVQSKTLQKLLKIYGINGKNIYRKNNYVTYIKESEQISDLLALMGANNAVLDFENIRA
                     VKETRNQINRVINCETANLDKIVDTSMRQINNIKILKKYKVIDQLPDNLRELAYLRLK
                     HSNASLKELGQMLDPPLGKSGVNHRLRKIEEIAEDLIEHTKRGVIK"
     gene            complement(8271..8696)
                     /locus_tag="PLD67_00070"
     CDS             complement(8271..8696)
                     /locus_tag="PLD67_00070"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT20952.1"
                     /translation="MVDEISAGGIVFFKNNILMLKKYNGDWVLPKGRVEENESLKETA
                     LREVYEETKAKVTPIKYLGKISYEFSRTCYLDKIHIRKEVHWYLMMARNMNCTAQKNE
                     GFIDAKFLPFERSLTIARYDDERKIIKKAVDDIKFHSYK"
     gene            complement(8828..9970)
                     /locus_tag="PLD67_00075"
     CDS             complement(8828..9970)
                     /locus_tag="PLD67_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOT20953.1"
                     /translation="MEKIKLAATSAFGLEAVVKRELTDLGYENIVTDNGWLYFDAEIQ
                     DICKTNINLRCADRVMLVMGQFEALSFEELFDKTYELPWEKWITKDGKFTVNGKSVKS
                     KLYSTPDCQRMVKKAISKKLCEYYDTDWMPETGAEFTILVSIHKDIATISIDTTGARE
                     GLFKRGYREKSMAAPLKETMAAALVKLSYWNKNRVLYDPLCGSGTIAIEAALIGRNIA
                     PGLNRNFASMEWPLVKDEYWKNEKIEARKRIDFDSEIKIYASDVSEKAIRIAKENAIE
                     AGVDDCIEFFVKDVTHIEKPMCSYGVLITNPPYAERIGDEEQLTKIHKKLGQVFGRDK
                     TWSVYVITSSVNFEKEFGRKADRKRKLFNGDMRVDYYQYFGEKMPE"
     gene            complement(9985..11409)
                     /locus_tag="PLD67_00080"
     CDS             complement(9985..11409)
                     /locus_tag="PLD67_00080"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT20954.1"
                     /translation="MNNEIEFSKQDREKFEYILDNIPDAITIVDNYGKVIFFNSTSEE
                     YFNLKKSDIIDKDLVEFFPTAILLRVLDSKTSFYNIYNSPRENSFVVSSAAPLYDRNG
                     NMIGALARDRDITEIVRLSELLNKTQSNLLKLEKEYSKVTSSGEAYFSKIISNNAAFI
                     EIINLCKNISKTPLNILLKGESGTGKELFAKAIHYESGRKGKFIPINCSAIPRELFES
                     EIFGYEAGAFTGAKSEGKKGKFEEAEGGTIFLDEIADLPLELQPKLLRILEDGVVTRI
                     GGNKPKTIDVRIISATNKDIRELINEDKFRKDLYYRLDIFQVNIPPLRERKGDIILLA
                     NRFLQEFCMEQGISIVEIPDEIMSIFSEYRWEGNVRELRNVIQRCAVIASQRGKNKIE
                     REYLPSYMQDIEVDEIKKLINLNEIKSYSGLEEYIQQIEKRIIIKTLEEMNYNKKETA
                     KKLQIPRATLYYKMDKYGISRDDK"
     gene            complement(11491..12642)
                     /locus_tag="PLD67_00085"
     CDS             complement(11491..12642)
                     /locus_tag="PLD67_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOT20955.1"
                     /translation="MNIDEIIKNSLTENKFIYCIFTTPRVKSQYKKITVRPIAIKDRE
                     YLQLEKFTETKAFHENLDYEVALDVLLNMVNEYRNINLFTTDADYQILVSKKGSIKIT
                     RSEPVKKLKKEEHNKKKQYLISENQPCDFLIILGIMNKDGKVYTKKYDKFKQINKFLE
                     IVDDSLAGKSIKDGFTIIDFGCGKAYLTFALYYYFYYIRRIKVKITGLDLKEEVIGFC
                     NETAKKLNYENLNFVYGDISDFEYKNKVNMIVTLHACDTATDAALVKAIAWDCDIILS
                     VPCCQHELFNKIENENLNPMLKHGLIKERMSSLITDSLRGLFLETKGYKVQLMEFISM
                     EHTPKNILIRAVKSNKNTESAEIEYEKFKKYWNLNNLFIENYYETIINP"
     gene            complement(12658..14340)
                     /locus_tag="PLD67_00090"
     CDS             complement(12658..14340)
                     /locus_tag="PLD67_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004840864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOT20956.1"
                     /translation="MSEFNNENENRESVNFIQKIINEDLEKGTYGNRVHTRFPPEPNG
                     FLHVGHAKSICLNYGLAKKNNGIFNLRFDDTNPTKEDDIFIESIIKDVKWLCPDWEER
                     IFYASNYFGKMYDYAVELIKKGLAFVDDLSADEIREYRGTLTEPGKESPYRNRTVEEN
                     LELFEKMKNGVFKDGEKVLRAKIDMASPNINMRDPVIYRILHESHYNTGDEWCIYPMY
                     DFAHPLEDAIEGITHSICTLEFEDHRPFYDWVLSNIDDFKDAPTKQIEFAKLFLSKTM
                     MGKRYLKQMVEEGYVDGWDDPRMPTIAGFRRRGYTPEAIHNFCEAIGVSKAQSVVDIA
                     MLEFAIRDDLGPKVPRTMAVLDPIKLIITNYPEDKVEYVETENNPDNPDMGSRMIPFT
                     REVYIEREDFMENPPKKYFRLFPGNEVRLRNAYFVKCVDFVKDEDGNVTEVHCTYDPE
                     TKSGSGFTGRKVKGTLHWVSAKHCIDAEVRLYDYMMEDEEYDKTNFLEKLNPESKVVL
                     KNCKVEPSMKEFKVGDRFQFLRHGYFIIDQDTTEDNLVFNRIVSLKSSFKGV"
     gene            complement(14579..15433)
                     /locus_tag="PLD67_00095"
     CDS             complement(14579..15433)
                     /locus_tag="PLD67_00095"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HOT20957.1"
                     /translation="MEIKKICIVGTGTMAKGIVQTFAQAGYPVIVKGRPGNTFDTLET
                     VIGKGLSKLVAKGKIEQSKMDEILGNITKTTTYEECADADLFIEAVAEDMVTKHAMFA
                     DIEAVAKEDAILSTNTSSLSITEIAAKSKNPGRIIGMHFFNPVPVMKLVEIIKGKLTD
                     DEVHAKVFELAQKIGKTPVSVEEAPGFVVNRVLIPLVNEGIGILADGVASAEEIDTAM
                     KLGANHPMGPLELGDLIGLDVCLAIMEVLYNEFGDPKYRPHPLLRKMVRANLLGRKTG
                     KGFYDYSK"
     gene            15595..16431
                     /locus_tag="PLD67_00100"
     CDS             15595..16431
                     /locus_tag="PLD67_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOT20958.1"
                     /translation="MIKKFLIFATKLINKTKEDDILALSAQLSYYLILSIFPFLILAI
                     SLMCGYSEYIYSILNSLSDVIPEEVHRIIYNVLKYSVASCSKPYLTISMLIIIWSATS
                     GSAAIINGINIAYGFNTRKNFLFLRIRGILFTLALMVSMQIIFAAVVAGRALLLFVKG
                     ISIFEGYNFIFIDILRFAVTFFIVLIILLASYKFLPYEKVKFSYAYPGAIFAAAGSIA
                     GSYLYSRYVSTRVVFINSIYGNLSGLFVFIIWIYILSIIFLMGAEVNYFAAKQKNGIN
                     GK"
     gene            complement(16492..17097)
                     /locus_tag="PLD67_00105"
     CDS             complement(16492..17097)
                     /locus_tag="PLD67_00105"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT20959.1"
                     /translation="MEQYFTENPTTEKEIYKFEWNVGKEVFYFYTSNSVFSKKGVDFG
                     SMLLVETILNENENFSGSILDLGCGYGPLGIIVAKKLRKSFVTMCDVNERALELSRMN
                     AKENKVDDRIKVIASSAFENIKDNYDIIMTNPPIRAGKNVVFSFYEGAYEHLNDGGKL
                     YVVIQKKQGAPSTKAKLESLFGNCETADKKSGYFIFRCIKK"
     gene            complement(17162..17980)
                     /locus_tag="PLD67_00110"
     CDS             complement(17162..17980)
                     /locus_tag="PLD67_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT20960.1"
                     /translation="MPRQARIFSDTGVYHIMMRGNDKGRIFLDDDDRRRFISTLFEKA
                     SEENTDIYAYCLMSNHVHLLLHDKEYNITRLMKRINVSFAYHFNKKYKRVGHLFQDRF
                     VSEIIDNNDYLLAAVRYIHNNPVTAGIVSSPEKYLWSSYNDYISSKKSSSLTTDKILN
                     IFSDNITVAIKQFIDFSSLDCDIKFIEFIEKSNEELRMEQEKRATETLDNILLLKGLC
                     INDLSKKENHNLRNKLTIYLKEEFNLSSRQIAAIIRISKGTVLKIMKEMKIGNN"
     gene            complement(18230..18676)
                     /locus_tag="PLD67_00115"
     CDS             complement(18230..18676)
                     /locus_tag="PLD67_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HOT20961.1"
                     /translation="MNFVEYEKECEKIRNDNKKYLEIFQSEMIEKGLSEKTIQSHLNN
                     IDFYINDFLLREEPIPMNQGYQYVDMFLGDYFIRKCMWSTPSTIKSTAASLKKFYKCM
                     LEHYMVKKEDYQELTETIKENMDEWLEDCSMYNDPDQDNPFYLFDV"
     gene            19198..19440
                     /locus_tag="PLD67_00120"
     CDS             19198..19440
                     /locus_tag="PLD67_00120"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20962.1"
                     /translation="MEKLDILELIEQKKYIQLKKELSEMNEVDISEILDPLDLQTTLL
                     VFRMLPKDTAAEVFSYISPEQKADIINAVTDKELKL"
     gene            19437..19622
                     /locus_tag="PLD67_00125"
     CDS             19437..19622
                     /locus_tag="PLD67_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20963.1"
                     /translation="MIVTIPEARSGRIRDLFIGHLSISAPANGEKIKFGIKNRVVIML
                     NGVAFPLIFQIQIESPN"
BASE COUNT         5558 a         3900 c         2788 g         7388 t
ORIGIN      
        1 tcgaaggttg gtatttgatt gtttaccata tccatgaaaa tcagccatgc ttcatcatgc
       61 tgaggacctt tgtttgttaa accgtatcct ccctgctgag ccagagattc tttcgagccg
      121 tattccgaat attcaagccc cttttgctcc attcctatgt caaacaagaa ttcttcatca
      181 gctccgtatt tttcaacgaa tatttttttc atcctgtgga ctccctgacc tacaactgtt
      241 ccaaagcctt ctcccttgga catctggtgc aagagttcga ctgatgcata aaagttgccg
      301 aaattcaatt ctaagccgcc tgtaatttcc ttgttgatta tacctctttc atagcattcc
      361 attgcaaatg ccgttgcagt accaaatgaa attgtatcta tgccataggt gtcgcaatag
      421 aagtttaatt ccaaaattgc ttccgggtca aaaatagcac aatttgctcc taatcctgcc
      481 gcattttcat attcgggacc gtctactgta actctatgtc ccttataagg tcctgtcttt
      541 atgaggaaat tatccgcacc tttggcgcag gccatagagc agccgtacca gcagccgtct
      601 tgaactccct gagtcaggta tttttcaata aataccgtgg atttaatatt ttgtgtgtcc
      661 ggatggcttc catattggaa attcataaca ggaagcaaat cataggcatc cattatttca
      721 ataatatttg ccgtgcctat ttttcgcata ttgttttgca aatggtctaa gtcatgaatt
      781 tccttatgat attttattcc ggttttatta attattgact ggtcaaaatt attgtttaaa
      841 ttgcctttta caccgctgta ttttacaatc agtgccttaa gctttttatt tcgaaatacg
      901 gttcctattc cacccctgcc tgcttgtttt agtcttgtta cttttcttct ggggtcatag
      961 aatgtaaaat ttaatattcc aaaacgggaa tgctcggcac ctgttcctgc ggttacaaca
     1021 gctacatttc ttttatcatc ttcattgtcc gcatacattt cggtaagctg ctctcccaaa
     1081 acatgtgcat caacagcttc caatggtgct tcttcaattg ttacaaggcc attattccca
     1141 tcaatatata caattatatc tttatctgat ttgccctgta tttcaagagc gtcccatccc
     1201 gaaaatttca aaagaggtcc aaaaaatccg cctacatttg aatctatggg aatatccgtt
     1261 aagggtgaaa tgctgcatac caatgatttt cctgttcctg catattgagt tataccgcag
     1321 acaggacccc ctgcaataat tatttcattt tccgggctat cccacttcgt ttcaggactt
     1381 gttgcattcc acagatacca caagccaaaa ccctttcccc ctacaaactt ttctttcatc
     1441 atttcggtta tcggcttaga ggttattgta ttgtctgaaa tattaacata cagtgttctg
     1501 tttgtatatc ctttgtctat ttctccgagt tcatatttat attcggttaa gactttatga
     1561 ttttctttca tttttataat atccattttg tcctcctatc tgttaaaaac cttatctatt
     1621 ggcatttctc ttgcttcctc atcgcttctt tcagctatat aaattgcctc cgtaggacat
     1681 tctttggcac ataatccgca tgctatacat ttaaaaggct ctatataatc atcatgctgc
     1741 ctcatggata agagagggca aaatcccaca cacataaaac aaccgacgca aattttcttg
     1801 tcaattctaa caaccccaaa cttgtccctc tttattgcca taacaggaca aatatcgatg
     1861 cattctccgc attgattgca aacatttatt tcaggggtgt cctctttttt gatttgtatg
     1921 catgactttt ccggattatc ctctttaaaa tatgttttag agcatatgct ttcgcaagcc
     1981 ccgcactgga tacatttact gtcgtcttta gccaaatatt tcatactgac ctcctcttgt
     2041 atgttaatat taattagatt tcattcattt ataatcactt atattatata aatattttta
     2101 atatatttca atttcttata gtattattaa tgtgctaata tttttctatt agttgctcct
     2161 tttctcgcaa tatcattctt caatatattt caaaatattt tttagtttgt ctccttcaaa
     2221 catatatttc ttattgcaga aatggcatac tgtttctatt ttcccgtctt cctcaattat
     2281 ttttttcagc tcattcttac ccaatgaaat taatgcctgc tccattcttt cctcggagca
     2341 gtcacaatta aaagaaacgg gaattctgtc gaaaacctcg atatcaaaat cggaaaatat
     2401 ttcttttgca atttcttcta gatctttttc gttattaaaa taatctgata ttgattttag
     2461 attattgagg gtattttcta atttaacaag gtcttcttcc tcaataaaag gcagtgtttg
     2521 aaccataaag cctcctgcgg ctttaatgct gtaatctata tccactaata cacccaaggc
     2581 tacggcagta ttttgctgtt ccgacaaata ataataaaat gctatgtctt ctccaatttc
     2641 tcctgttacc aaatcggtgc ttcccacata gggctccttc aatcctatat ccatcataac
     2701 ggtgagcttg ccttgagtcc cgacagctcc tcctacgttt aatttaccgt tttctttcaa
     2761 gtctaaatca acattcggat tgccaacata ccccttaaca tggcctgaat tatccgaaac
     2821 aaccaaaatt gtacctatgg gacctccacc gtttatatta attgttaatc tgtctttttc
     2881 gtttttcatc atatagccca tcatgagacc ggctataaga gtcctgccta aagcagcaga
     2941 agctgtcggt gtcgtattat ggtgctgcct tgcttcttcc accgtatttg ttgcccttac
     3001 cataaaaagt ctaaaagtat gttttttatc cattgctcta atcatgtaat ccattgttat
     3061 gtcctttcat tgtcaaattt gcatgcggaa aaaactgccc gcaaagaggt gtcagaatat
     3121 tctttttcat tgtagtcatc aaatatctcg atattttcaa atcctgcatc ctcaagtaag
     3181 tctattatct catcaactgt atgaatatat tgagtttgtt cttcagtaat tcttgtatat
     3241 ttatctgtgt ccttaataaa aaagtttatt tcgatattta ttttttcatc attaacaagt
     3301 tcattttccc aaacataaaa aatatctccg tcatcataaa cataagtatc acaaaacatt
     3361 ttatcatatt tgtattttgt gctcatgtca aaaatgaatt tggagccttc atttaaatga
     3421 tggtaaatcc tttggaaaaa agatttcact tcttcctttg tgaggtaatt tatgccgtcg
     3481 cagcagcaaa ttgctgcttc aaatttttca tctatttcaa aatcaaccat attttgattc
     3541 aataatcgaa ccatagggct ttgcgccagc ttctcataag ccctcataag catgtcctca
     3601 gacaaatcaa aggcagttac cttgtaattg ttatcggcta aaagctttgt tatgcctccc
     3661 gtgccgcagg cagcttccaa gatttttctt tttcccttta atttcttcat tataaaggat
     3721 gtccactttt cgtaatcgat atccataagc aaatcataaa tttgagaaaa ttctttatag
     3781 ctgtttttca tttacttttc ctttactttt tccttttctt tttcttagct gtcattaatc
     3841 ctccgattct gcctgtttcc ttggcagtta atcctcccca gccgaacttt ttaactttat
     3901 ccgtaagtcc aagctcttct gctatttcca tcttcattat atcgtttggt gtcggttctt
     3961 ttttcttctt gtttttcttg ttcttctctt tcttttcagc cataataccc tccttgtaat
     4021 attatggctt ttaaaaggaa acttattcta ataatctgtt tatctatctc tcttagtgag
     4081 taaaattgcc cttatttctc ttaaaagcaa aaatattttc ttcagagtaa attgtatact
     4141 tattaaaatt ataaaaaaca ttacaacggc tattactcta aaatcaaaat ctaacatatt
     4201 ttctccttga aatgagacca tcttttttca ataatatcac aagaaaaaaa ccatttcaag
     4261 aaaaaacccc ctgggtcagg gggaaatata tatagttttg tatattattc attaacaaca
     4321 ataaccgcag cacatgggga ttcagcgata accctttgag ttaccgagcc cacaaaaaat
     4381 cttgtgaatt ttgaaaatcc tcttcttccc attacaatat aatcatattg acaatcgtgt
     4441 gccattctta ttatttcttc agcaggttct ccggaaataa cctttttttc aaatttaacc
     4501 ccttctatat ccagaccttc tataacttta tccagtattt cagcttgagc taatttgtga
     4561 ttatttacca cattatcata gttatagtgt actctgaaac tataatcacc aatgttaaac
     4621 atatcgggtg tatttgccac cgataaaaat gtaatctcac tcccatactt ctttgcaatt
     4681 tctatagctc tttcagatgc ctttatcgaa gccggcgagc cgtcaaccgg taccaatatt
     4741 ttctttatat catacataat taatttatct cccttcatta atattgtttg atatatatat
     4801 acctgtttaa attccaatta aacatttttt aacaaaattt attgtttata attcttttga
     4861 ttgattatgt gcagtttcag tgatagaata tagcgaagta aaaatttctc ctctttgggt
     4921 aaggggtacg gattaaatta cataaatttg attaggagtc atagaaatag atggaaacaa
     4981 aaaaagaaac cataataaaa gctgccggag cagcttttcc tcaaaccatt cctattttgg
     5041 caggctatgt attcttaagc atagcctatg gtatttatat gaatgctttc ggattcaatt
     5101 ttttatatcc catgtttatg agcatgttaa tctatgcagg tacagttcaa tttgttgcag
     5161 taggtcttct gatggcagtc ttcgacccta taaatgcttt tgttctgaca cttatgatta
     5221 atgcacgcta tttgttttac ggcatttcta tgttggaaaa atatcgggtt aagggaataa
     5281 gaggagcctt tttgattttt agtatgagtg atgaaacatt ttcaatcaat tgcacggcaa
     5341 atatcccgga agatgtcgat aagggttggt tcatgttctt tgtaactctt tttaattact
     5401 gctattgggt atcaggctcc atacttggcg gattgttcgg tgaattgatt gaatttaata
     5461 caaaggggct tgaatttgca atgacagcaa tgttcattgt aatctttatg gagcagtggc
     5521 tgaaagaaaa aagtcatagc agctcgctgt taggattggg gatatctttg ttatgcctaa
     5581 ttatctttgg cagcaaaaac tttataatac cttcaatgat tttaatttta ttaatccttt
     5641 ctctaaaaag agaacttttt atggaagcag gtgaatcaaa atgacgatga ctgtcggaca
     5701 acatattata acaattgcca tggtagtact tggtacaatg attacccgtt ttcttccctt
     5761 tataattttt cctgccgata agccaacacc aaaatatata cagtatttgg gaaaggcgct
     5821 cccgccggct tccctgggac tgcttgtagt gtacagtctt aaggatgtaa atatatttac
     5881 gggcagtcgc ggtattccgg agctgattgc cgttattaca gtacttgtta tccacctgtg
     5941 gaaaagacag atgcttttat ccattgctgt tggaacaata acttatatgc tcttggttca
     6001 atttgtacta tgactttcta ccattaaaaa acggctccct gtcggaagcc gttaaaaagt
     6061 tgaattatta gtgcggataa caggatttga acctgcacag gttgccccac acggacctga
     6121 accgtgcgcg tctgccaatt ccgccatatc cgctggtcac tgaaatatgt tatcacaaat
     6181 atataatctt tgcaatattt ttttcgtgct gtctcaactt tctcaataag ttatcaatta
     6241 ttcaaataat acaacatcat actttttaat taattcttct tttttacttt tataaacttc
     6301 ctcttgttta attacaaaat aattgtcttt tacttcctgg taaacttctt caaattctgc
     6361 acttctagca ggcatatgct caattaattt aattatgtga tatccaaact gtgtttttac
     6421 aggtggactt atttcacctg cgcgcattga aaatacagca tcttcaaatt cttttaccat
     6481 ttgccctttt ccgaactgtc ccaaaaatcc tccgtcttgc ttggaaggac agtttgaata
     6541 ctctttagcg gcatcctcaa aagacaaacc atctgttata tcttccaata tttcatttgc
     6601 tctttcctct gtatcaacca agatgtggct tgcttttacc atttcgggtc tattgtaaaa
     6661 atacttgttt ttttcataaa agtctttcaa ttcttcgtcg gatattgtaa ctttgttgaa
     6721 catttttctt aagttgtatt gttgcagcat cgactttttc atatcgttga atacttcgat
     6781 aaattcttct tcattttcca gattgttttc taccgcatcc gaatacataa gttcatgcat
     6841 aaccaattca tcaataagct tttttcttcc ttcttctcct tgaaaatgca ctgcattttg
     6901 tcctaaattc cgtaataaat tttttaagtc tgattctttt atttctcttc cgtctactat
     6961 agctaatact ttattctcca ttttttctct ttcgtcataa aataagaggg tcttttccgc
     7021 gccctcttta ttattcttct tcttccataa attccaataa attagtcaac tcagcaatac
     7081 atttgtcctc gtctgaacct ttggtgatta ttactaattc atcgcccttc atggctccca
     7141 aagccattat gctgataata ctcttgccgt ttgcttcctg tatacctttt ttgattataa
     7201 tttcactgtc aaaatttctt gcctttttaa caaataaagc cgcaggtctg gcatgcagtc
     7261 caatcttatt ttttaacact actttttgtg atctcacttt attacacctc tctttgtatg
     7321 ctcgattaaa tcttcagcaa tttcttctat ttttcttagt ctgtgattta ctcctgattt
     7381 tccaagggga gggtctaaca tttgacccag ttcctttaaa cttgcattgc tgtgtttaag
     7441 tcttaagtat gcaagttccc ttaaattatc cggaagttgg tctataacct tgtatttttt
     7501 tagtatttta atattgttta tctgcctcat tgatgtgtct acaattttat ccagatttgc
     7561 agtttcacaa ttaattactc tgtttatttg atttcttgtt tccttaacgg ctcttatgtt
     7621 ttcaaaatcc aagacagcgt tatttgcgcc catgagagct aacaaatcgg aaatttgctc
     7681 gctttcctta atatatgtca catagttgtt ctttctgtaa atattcttac cgtttattcc
     7741 atatattttc aaaagctttt gcaaagtctt gctctgtact tccttgttgc ttacaaattc
     7801 agcatgatat gatttttcag gattgcttat cgaaccgctg cccataaatg aaccttttat
     7861 aaaggctctc ctgcataagt cgcttgccag taattcttca ggtacttcat aattaaaatt
     7921 cattacatta aaatcattat ctttaataat ttgactgtct gaaagaaatt gcttggttaa
     7981 tttttcatct attttaatta tataattatt ttgctttttc aatcgattgc ttttagaaac
     8041 actgacatct gcatttatat tatgggctat ttttaaaacc ttgaatatcc gtcttgcaac
     8101 tgctgcattt tcagtcaaaa attcaaattc taccttattt agacccttaa ttgtgatata
     8161 tcccacagtt ctgacaaatg ctgccgcttc agcattagca cataatacat cacttatttc
     8221 tgttctggac agttcatctt taactctcga tgaaaatgtc atggcatcac ctacttatat
     8281 gaatgaaatt taatatcatc tacagctttt ttaatgattt tcctttcatc gtcatatcga
     8341 gctattgtca atgatctttc aaagggcaaa aattttgcat ctataaatcc ttcatttttt
     8401 tgagcagtgc agttcatatt tcttgccatc attaaatacc aatggacttc ttttcttatg
     8461 tggattttat caaggtagca ggtgcggctg aattcgtaac ttattttacc taaatactta
     8521 ataggagtta cttttgcttt cgtttcttca tatacttccc ttaaggcagt ctctttcaag
     8581 gactcgtttt cttcaactct gccttttgga agaacccaat cgccgttata cttttttaac
     8641 atcaatatgt tgtttttaaa gaaaactatt cctcctgcac ttatttcatc taccatactt
     8701 gcctcacctt ttaatattgt agtctttata ataccacaat attaagaaaa atcaaacata
     8761 tatttacata ttttaagttt acaataaaaa taaatatatt tcaatctttt catttcggtg
     8821 cgcagtctca ttcaggcatt ttttctccga aatactgata gtaatcaacc ctcatatcgc
     8881 cattgaaaag ttttcttttc ctgtctgctt ttctcccaaa ttctttttca aagtttactg
     8941 aagaagtgat tacatatact gaccaagttt tgtccctgcc aaaaacttgc cctaattttt
     9001 tatgaatttt agtaagctgt tcttcatcac ctattctttc cgcatatggc ggattggtta
     9061 tcaatactcc ataggaacac atcggttttt caatatgtgt aacatccttt acaaaaaact
     9121 cgatacagtc atccactccc gcttcaattg cattttcttt tgctattctt attgcctttt
     9181 cactcacatc tgaagcatat attttaattt ctgaatcaaa atcaattctt tttcttgctt
     9241 caatcttttc atttttccaa tattcatctt taaccaatgg ccattccatg gaagcaaagt
     9301 ttcgatttaa accgggagct atatttcttc cgattaatgc agcttcaatt gcaattgtac
     9361 ctgaaccgca taatgggtca tagagcactc tgtttttatt ccagtaactg agctttacca
     9421 aagctgcagc catagtttcc tttaaaggtg ccgccattga tttttctctg tatcctcttt
     9481 taaacaatcc ttcccttgcc ccggttgtat ctattgaaat cgttgcaatg tctttgtgaa
     9541 ttgaaactaa tatggtaaat tcagcccctg tttccggcat ccagtccgta tcataatatt
     9601 cacagagttt tttggaaatt gcctttttaa ccatcctctg gcagtcaggg gtgctgtaga
     9661 gttttgattt gacggatttt ccgttaacag tgaactttcc gtcttttgta atccattttt
     9721 cccaaggaag ctcataagtt ttgtcaaata attcttcaaa acttaatgct tcgaattgtc
     9781 ccataacgag cattactctg tcggcacacc tcaaattgat atttgttttg catatatcct
     9841 gtatttctgc atcaaaatac agccacccgt tgtccgtaac tatattttca tatcctaagt
     9901 ctgtcaattc tctttttaca actgcttcaa gcccgaaggc cgatgttgca gctaatttta
     9961 ttttctccat actttccttt cttgtcattt atcatccctg cttattccgt atttatccat
    10021 cttgtaatac aaggtggctc ttggtatttg taatttcttt gctgtttctt tcttattata
    10081 gttcatttct tccagtgttt taatgataat tcttttttct atttgttgaa tgtattcttc
    10141 caatcccgaa tagcttttta tttcatttaa attaataagc ttcttaattt cgtcaacttc
    10201 gatatcctgc atgtatgaag gaagatattc tctttcaatt ttattctttc ctctctgaga
    10261 tgctattacc gcacatcttt gtattacatt tctcagttct cttacattac cttcccatct
    10321 gtattcggaa aatatactca ttatttcgtc ggggatttcc actatgctaa taccctgctc
    10381 catgcagaat tcctgtagaa acctgtttgc taaaagaatt atatctcctt ttctctccct
    10441 gagaggaggt atgtttacct gaaatatatc aagtctgtaa tagaggtcct tcctgaattt
    10501 atcctcattt atcagttctc ttatatcctt attcgtggca gaaattattc tgacgtctat
    10561 tgtctttggc ttatttcctc caattcgagt tactacgcca tcttccaaaa tccttagaag
    10621 cttgggctga agttctaagg gcaagtctgc tatttcatcc aaaaatatgg ttcctccttc
    10681 agcttcctcg aattttcctt ttttcccttc agattttgct ccagtaaagg cgcctgcttc
    10741 atatccgaaa atttcactct caaacaattc tctcggtatg gcactgcagt ttatgggaat
    10801 aaattttcct ttccttccgc tttcataatg aattgctttt gcaaagagct ccttacccgt
    10861 gccgctttct ccttttaaca atatgtttag aggtgttttg gatatgtttt tgcacaaatt
    10921 tattatttca ataaaagctg cattattgct aattatttta gagaaatatg cttctcccga
    10981 ggaagttact tttgaatatt ccttttcaag cttcaataaa tttgactggg ttttattcag
    11041 tagttcgctt aatcttacga tttcagtaat gtccctgtcc cttgccaatg caccaatcat
    11101 gtttccattc ctgtcataaa ggggagcagc actggacaca acgaagctgt tttccctggg
    11161 gctgttgtat atattataaa aagatgtctt gctgtccaat actcttaaca atattgcagt
    11221 gggaaaaaat tcaacaagat ccttgtctat tatgtcgctt tttttcaaat taaaatattc
    11281 ctctgatgtt gagttgaaaa atatcacttt gccgtaatta tcaactattg ttatggcatc
    11341 aggaatgttg tccagtatgt attcaaattt ttctctgtct tgtttgctga attctatttc
    11401 attattcatg taatcccccc tggaaacaat aatattttgg gcaagctctc atctgttgca
    11461 tataccaaag tggtatgcaa taagttaaaa ttacggattt atgattgtct cgtagtaatt
    11521 ttctataaaa aggttattga gattccaata tttcttgaat ttttcgtatt caatttccgc
    11581 tgattctgta tttttatttg acttaactgc tcttatgaga atgtttttag gagtatgctc
    11641 cattgatatg aattccatta actgcacctt atacccctta gtttccaaaa acaaacccct
    11701 caatgaatcg gtaattaagg aggacattct ctcctttatc agcccgtgct taagcatggg
    11761 atttaaattt tcattttcaa ttttattgaa gagttcatgc tggcaacaag gtacggacaa
    11821 aataatatcg cagtcccagg caattgcctt tacaagggct gcgtctgttg ccgtatcgca
    11881 tgcatgaaga gttactatca tatttacttt gtttttatat tcaaaatcac ttatatctcc
    11941 atatacaaaa ttaagatttt catagttaag ttttttggct gtttcattgc aaaatccaat
    12001 cacttcttct tttaaatcga gccctgttat tttcactttt attcttctaa tataataaaa
    12061 ataataatac agggcaaatg taagatatgc ttttccacag ccaaaatcaa taatcgtaaa
    12121 gccatcctta atactttttc ccgccagtga atcatcaaca atttccaaga atttatttat
    12181 ttgtttgaat ttatcatact tcttggtata aaccttgcca tctttattca taattcccag
    12241 gattataagg aaatcacatg gttggttttc acttatcaaa tattgttttt tcttgttatg
    12301 ctcttctttc ttcagctttt ttacaggctc acttcgagta atctttatac ttcctttttt
    12361 gcttaccaat atttgataat ctgcatctgt tgtaaataaa tttatattgc gatattcatt
    12421 aaccatgtta agcaagacat caagagcaac ctcatagtct aagttttcgt ggaaagcttt
    12481 tgtctcggta aacttctcca actgcaggta ctccctgtct tttattgcaa taggccttac
    12541 agttattttt ttatattggg acttaaccct tggggttgta aaaatacaat atatgaattt
    12601 gttttctgtt agactgtttt ttattatctc atcaatattc aaaataccac ctgtaattta
    12661 cacaccttta aagctgcttt ttaatgatac tattctgtta aataccaaat tatcttctgt
    12721 tgtatcctgg tcaataataa aatacccgtg tcttaaaaat tggaatctgt cacctacttt
    12781 gaattccttc atagaaggct caaccttgca gttctttaac acaacctttg attccgggtt
    12841 taacttttcc aagaaattgg ttttgtcgta ttcctcatct tccatcatgt agtcataaag
    12901 tcttacttcc gcatcgatgc aatgttttgc tgaaacccag tgtagagtcc cctttacttt
    12961 tctgccggta aagcctgatc cgctctttgt ttctggatca tatgtgcaat gaacttcagt
    13021 aacatttccg tcttcatcct ttacaaaatc aacacatttt acaaaatatg catttctaag
    13081 cctcacttca ttgccgggga agagcctgaa atatttcttc ggaggatttt ccatgaaatc
    13141 ttctctttca atatatactt cccttgtaaa aggtatcatt ctacttccca tgtcagggtt
    13201 atcggggttg ttttccgttt caacatattc aaccttatct tcgggataat ttgttattat
    13261 aagctttatt gggtctaaga cagccattgt tctcggaact ttaggtccca aatcatctct
    13321 tatggcaaat tcaagcatag caatatcaac aacgctctga gctttcgaga ctccgattgc
    13381 ttcacagaaa ttatgtattg cttcgggagt ataacctctt cttctgaatc ctgcaatagt
    13441 aggcatacga gggtcatccc agccgtccac atacccttct tcaaccatct gtttcaaata
    13501 tcttttgccc atcattgttt tcgataaaaa tagtttggca aattcaattt gttttgtggg
    13561 agcatccttg aaatcgtcta tattgcttaa cacccagtca taaaacggtc tgtggtcttc
    13621 aaattccaaa gtacatattg agtgtgtaat tccttctatg gcatcctcca aaggatgagc
    13681 aaagtcatac atggggtaaa tacaccactc atcgccggtg ttgtagtgag attcatgaag
    13741 aatgcggtaa ataacagggt ccctcatatt aatattggga cttgccatgt ctatttttgc
    13801 tcttagaact ttttcaccgt ctttgaatac accgtttttc attttttcaa aaagctctaa
    13861 gttttcttca accgttctgt ttctgtaagg gctttccttg cccggctctg tcaaggtacc
    13921 tctgtattca cggatttcat ctgccgataa atcatcaaca aaggctaatc ccttctttat
    13981 cagttcaact gcataatcgt acatcttacc aaagtaattt gaagcataaa aaattctctc
    14041 ttcccaatca gggcaaagcc atttaacatc cttgataatt gattctatga atatatcatc
    14101 ttcctttgtc ggatttgtat catcaaatct taaattgaaa attccattat tcttttttgc
    14161 caatccgtaa ttcaagcata tggatttggc atgacccaca tgcagaaatc cattaggctc
    14221 cggaggaaat cttgtatgta ccctattccc gtaagtgcct ttttcaaggt cctcattaat
    14281 tatcttttgg atgaaattaa ccgattccct gttttcattt tcgttattaa attcgctcat
    14341 tttttcctcc cgcaatatat ataaacaaaa taatattatt aaacctaatt ataattaaca
    14401 aatagctata attcaagttt ttcttcaata tttattatat cctgtcttat atacaaataa
    14461 tcatccggtt atttctttag tttgactcaa tatcattaaa atacccggtg acaacaataa
    14521 acaaaaagaa acggtcataa ccgttcctct ttctgccatc ataagcgtaa aaggatctct
    14581 atttgctgta atcgtagaat cctttgcctg tttttcttcc aagcagattt gctcttacca
    14641 ttttgcgaag taatggatgc ggtctgtact tagggtctcc gaattcatta tagagaacct
    14701 ccataatggc aagacaaaca tcaagtccta ttaaatctcc gagttctaat ggtcccatag
    14761 gatggtttgc acctaatttc attgcggtgt ctatttcttc tgcactcgca actccatctg
    14821 ccaatatacc tataccttca ttaaccaatg gaatcaatac tctatttaca acgaatccgg
    14881 gtgcttcttc aacacttacc ggagtttttc ctattttttg agctaattca aataccttag
    14941 catgaacttc atcatcagta agttttcctt tgattatttc aactaatttc ataacaggaa
    15001 caggattgaa aaaatgcatt cctattattc tgcccggatt ttttgatttt gctgcaattt
    15061 cagtgatgga caatgatgat gtattagttg atagtattgc atcttcttta gctacagctt
    15121 ctatgtcggc aaacattgca tgtttcgtta ccatgtcttc tgcaactgcc tcaatgaata
    15181 aatcagcatc cgcgcattct tcatatgtag tggttttcgt aatatttccc aaaatttcgt
    15241 ccatcttaga ctgttctatt ttaccctttg ctaccagctt gcttaatccc ttgcctatta
    15301 cggtttctaa tgtgtcaaat gtattgcctg gtcttccttt aactataaca ggatatcctg
    15361 cctgtgcaaa tgtttgtaca atacccttgg ccattgtacc ggttccgact atgcatatct
    15421 ttttaatttc cattttatcc tccaattatt tttttttgcc tgttttaatt ataacattac
    15481 gatataaaat aaatcaagca ttttaagaat acttgatgac ggtccggttc ccttattatt
    15541 atggtagatt cataataact gtattatttg ttaataataa tgtagggtga atcgatgatt
    15601 aaaaaatttt taatttttgc aacaaagctt ataaataaaa caaaggaaga cgatattctc
    15661 gcactgagtg ctcaattatc atattattta atattgagta ttttcccctt tcttattttg
    15721 gcaatatctt tgatgtgcgg ctatagtgaa tatatctaca gtatcttaaa ttccttatca
    15781 gatgtaattc cggaagaagt gcacagaatt atttacaatg tgttaaaata ctctgttgca
    15841 agctgcagca agccttactt aaccatcagc atgctgataa taatatggtc ggctacttcg
    15901 ggaagtgcgg ctatcataaa tggaattaat attgcatacg gattcaatac cagaaagaat
    15961 ttcttattct taaggataag aggaattttg tttactctgg cactgatggt ttcaatgcaa
    16021 attatttttg cggcagttgt agcaggaaga gctttattgc tcttcgtaaa aggaatttcc
    16081 atttttgagg gctataattt tatatttatt gatatcttaa gatttgctgt tacatttttt
    16141 attgtattaa taattctatt agcttcatat aaatttttgc cctatgaaaa ggttaaattt
    16201 tcatacgcat atccgggagc aatttttgcg gcagcaggct caatagccgg ttcttacctc
    16261 tattcccgct atgtaagcac cagagtcgtt tttattaatt ccatttacgg aaatttgtcc
    16321 ggattatttg tatttattat atggatttat attttatcga ttatatttct gatgggagct
    16381 gaagtaaact attttgcagc aaaacaaaag aatggtatta acggaaaatg accagagggg
    16441 acggttcttg gtggacagtt ttcaaggcgg gtggataatt aattctgttg gttatttttt
    16501 tatacaccta aaaataaaat accctgattt tttatctgct gtttcacagt tgccgaataa
    16561 actttcaagt tttgccttgg tgcttggtgc tccttgtttc ttttgaatta caacataaag
    16621 cttaccgcca tcgtttaaat gttcatatgc accttcataa aatgaaaata caacattttt
    16681 gcctgccctg ataggagggt tggtcataat aatatcataa ttatccttaa tattttcaaa
    16741 tgcagaagaa gcaataacct ttattctgtc atcaaccttg ttttctttag cattcatcct
    16801 tgacaattca agtgctcttt cattcacatc gcacatagta acaaaggatt ttcttaattt
    16861 tttggcaaca ataataccca aggggccgta tccgcaacct aagtctaaaa tactgccgct
    16921 aaaattttca ttttcattta aaattgtttc tactaaaagc atggagccaa aatccactcc
    16981 ttttttagaa aaaacactgt tgctcgtata aaaataaaaa acttctttcc ccacattcca
    17041 ttcaaatttg tatatttctt tctctgttgt cgggttttca gtaaaatatt gttccatttt
    17101 tctctcctta agtccaaagg ggacggttct ccccggacat ctttatcaac tatatatatg
    17161 attaattatt cccaatcttc atttctttca ttatttttag tactgtgcct ttacttattc
    17221 ttattattgc agcaatttgt ctagaagaaa gattgaattc ttctttcaaa taaattgtta
    17281 acttatttct taagttatgg ttttcttttt ttgataaatc attaatacat aatcccttta
    17341 acaataaaat attatctaaa gtttccgtag ctctcttttc ttgttccatt ctcaactctt
    17401 cattgctttt ctctataaat tcaataaatt taatatcaca atctaaactg ctaaaatcaa
    17461 taaattgttt aattgctaca gttatattat cagaaaaaat atttaatatt ttatcagttg
    17521 ttaagctact gcttttcttt gaacttatat aatcattgta actactccat aaatactttt
    17581 caggtgaact aacaatgcct gctgttaccg gattgttgtg tatataccga acagcggcta
    17641 acaaataatc attattatct ataatttcac taacaaacct atcctgaaac aaatgtccta
    17701 ctctcttata tttcttattg aagtgatatg caaaacttac attaattctt ttcattaacc
    17761 ttgtaatatt atattcctta tcatgcaaca ataaatgaac atgattgctc attaaacaat
    17821 aggcataaat atctgtgttt tcttcagaag ctttttcaaa cagagtagaa ataaaacgtc
    17881 ttctgtcatc atcatcaaga aatatccttc ctttatcgtt accacgcatc atgatatgat
    17941 aaacacctgt atcactgaat attcttgctt gtcttggcat actaacacac tccttaaatt
    18001 atcttcttgt aacaaaagga ttatgctctg ctctgttgta attattgttg ttataaccat
    18061 atatcattaa aatatttatg tcaacgctaa tttgctaaat attggaaatt aattaattta
    18121 cctgcctaat cttaaaatag ttttaatata aaacctcatg gtgccaaaag gaaccgtccc
    18181 cattagtcaa aaagaaccgt ccacgttggt catcccaatt tgtcattgct tacacatcaa
    18241 ataggtaaaa tggattgtcc tgatctggat cattatacat actgcaatct tctaaccatt
    18301 catccatatt ttccttaatc gtttcagtca gttcttgata atcttctttc ttcaccatat
    18361 aatgttccaa catgcattta taaaattttt tcaaactggc tgctgtactc tttatagttg
    18421 acggtgttga ccacatacat ttccttataa agtaatctcc taaaaacata tctacatatt
    18481 gataaccttg attcattgga atcggttctt cccgaagcaa aaaatcattt atataaaaat
    18541 caatattgtt taaatgtgac tgaattgttt tttccgaaag acctttttca atcatttccg
    18601 attgaaatat ttcaagatat tttttattat catttctaat tttttcacat tctttttcat
    18661 attctacaaa attcataatt tctccttttc aaacaatgga aatgttattt catccttgtt
    18721 tatatgttct tattaatgta attatatgct ctaaatatat cgctgtaaag atatatagag
    18781 taaatatgca gcttgcttta tactgaaaat aaaatatttc atttactcat gaagaattgg
    18841 ttatcaaaaa ggaagaaaga accgttcctt tcaggtcttg aatttgtccg gagagaaccg
    18901 tccccacgtc cccactggtc acactggtca aattattttt cataataata aaatatattt
    18961 tttatatcat attaaatatg ttataatgaa tacaatttat gaagcaagcc gattctgcgg
    19021 cttgtactga tgatagaata tacaataatt tctaaaagga gtgataaatg tggactctga
    19081 acctttgtct gataatttaa caagaaaaca taacaaccct attaaggaag ccatgtttat
    19141 cgccgcgtct tctttaatgg gataggacaa taagtcttta tcataaggag ggtgcaaatg
    19201 gaaaaattag atattttaga actaattgag caaaaaaaat atattcagtt aaaaaaagaa
    19261 ctttctgaaa tgaatgaagt agatatttca gaaatattag atcctttaga cctccaaaca
    19321 accttgctcg ttttcaggat gcttccaaag gatacggctg cagaagtttt ttcttatatt
    19381 tcaccggaac agaaagcaga tattattaat gcagtgacag ataaagaatt aaaattatga
    19441 tagttactat tccggaagca aggtctggaa ggataagaga tctttttata ggccatctgt
    19501 caattagcgc tcctgcaaat ggagaaaaaa ttaaatttgg aataaaaaat agagttgtga
    19561 ttatgctaaa tggagttgca tttccactta ttttccaaat ccaaatagaa agtccaaatt
    19621 gagtcatcgc tgag
//
LOCUS       DAOSOW010000002        17966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_3149_length_17966_cov_4.036123, whole genome shotgun sequence.
ACCESSION   DAOSOW010000002 DAOSOW010000000
VERSION     DAOSOW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 17966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17966
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3149_length_17966_cov_4.036123"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..503
                     /gene="rplM"
                     /locus_tag="PLD67_00130"
     CDS             75..503
                     /gene="rplM"
                     /locus_tag="PLD67_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOT20964.1"
                     /translation="MKSFLAKPNEVERAWYVIDAEGKTLGRLATEVAKLLRGKHKAIY
                     TPHVDTGDFVIVVNAEKVVLTGKKLDQKLYRHHSLYPGGLKEVPYRNLFRSKPTQPVY
                     LAVKGMLPKNSLGRKMLKKLKVYAGPEHNNQAQKPQVYEF"
     gene            522..911
                     /gene="rpsI"
                     /locus_tag="PLD67_00135"
     CDS             522..911
                     /gene="rpsI"
                     /locus_tag="PLD67_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOT20965.1"
                     /translation="MEAQYRGTGRRKKAIARVRLVPGTGKININERDLDDYIKYETLR
                     NLVREPLMITDTFGQYDVLVNVKGGGYTGQAGAIRHGISRALLQVDKELRPLLKKAGF
                     LTRDSRMKERKKYGLKKARRASQFSKR"
     gene            1054..1554
                     /locus_tag="PLD67_00140"
     CDS             1054..1554
                     /locus_tag="PLD67_00140"
                     /inference="COORDINATES: protein motif:HMM:NF017941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HOT20966.1"
                     /translation="MKRNNRFITRTALIAAAYAALTYAFAWMSYEQIQFRISEVLVLF
                     AFIEPRYGLGLVLGCILANLASPFGVIDIAVGSFATFLAIVFIVKIRKLFGLNKKALI
                     IASLGPVITNALLVGAELTYLFNTPFLLNALYVGAGEFAVVVFIGTVVVNLIMKNNVL
                     VEKLSF"
     gene            complement(1693..2568)
                     /locus_tag="PLD67_00145"
     CDS             complement(1693..2568)
                     /locus_tag="PLD67_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOT20967.1"
                     /translation="MHLELKDKEIDYEVYITKSKEDTRNFAENKCKENIQAVLYSCGG
                     DGTLHEIINGACRYKHVSIAIIPIGSGNDFIKNFKNSANFSDINLQTEGESIELDLIK
                     VNNEYSANVLNIGLDADAAYNMDKFRKFPFIQGTTRYYLSMLYSLFGKLGKHIEVNTE
                     NGVINGTFLIGVIANGQYYGGGYKCAPKAKVNDGLLDLCFVEKISRAKILNIIGSYKK
                     GEHLDNPKINKYIIYDKINQANIKFDKQTNVCMDGEIYTYNELNISTEKNALKFWIPK
                     GVQLQTQQIENHSFV"
     assembly_gap    2768..2867
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2974..3603)
                     /gene="upp"
                     /locus_tag="PLD67_00150"
     CDS             complement(2974..3603)
                     /gene="upp"
                     /locus_tag="PLD67_00150"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOT20968.1"
                     /translation="MNNITVVSHPLIQHKLTMLRDKNTPPKDFRELVREISMLLAYEV
                     TRSLPLKDIDIETPICKTTGKILSGADIAIVPVLRAGLGMVDGMLDLIPNAKIGHIGL
                     YRNEETLEPVEYYCKLPSDIDKRIVIITEPMLSTGGSMNGALNMLKKRGAKKIKSIHL
                     VCAPEGLKRVTETHPDVEIYTAAIDEKYNEIGYIVPGLGDAGDRLFGTK"
     gene            3763..4128
                     /gene="acpS"
                     /locus_tag="PLD67_00155"
     CDS             3763..4128
                     /gene="acpS"
                     /locus_tag="PLD67_00155"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOT20969.1"
                     /translation="MIKGTGIDITEVERIKKNMGNIRFLEKIYSNRELEYLKTRKFNP
                     QTAAGMFAAKEAVAKCLGTGFSNFGPCHIEILKDENDKPYVFLLSNALKRAEELNIKN
                     IYISISHTQEYAVAHCVAE"
     gene            4290..4787
                     /locus_tag="PLD67_00160"
     CDS             4290..4787
                     /locus_tag="PLD67_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20970.1"
                     /translation="MRLIIFILVLLMALSGCTAEDKPFILPVNYSANALMKVYTDKTE
                     NDYKINIICRDGNYSIRTETENEAWNCAYVSGNRCILNNDKFPESSVTIENYKLNDTL
                     LHDFDFGKFDVFEDIPEELVYWDGEYKHVLNFSKDNMLPKTIFIYKYDKLVKAIQYEK
                     INIEE"
     gene            4887..5162
                     /locus_tag="PLD67_00165"
     CDS             4887..5162
                     /locus_tag="PLD67_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HOT20971.1"
                     /translation="MAENKKVLISIPANLLRELDNAVKEEGTNRSDFIRKSIRFYLIE
                     KRKLEIRNKMKAGYLEMSKINKSITEEYSEGDYEDFLSYETKLLGSE"
     gene            5164..5514
                     /locus_tag="PLD67_00170"
     CDS             5164..5514
                     /locus_tag="PLD67_00170"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOT20972.1"
                     /translation="MIVKRGDVYYADLSPVIGSEQGGVRPVLVVQNDIGNRYSPTVIV
                     SAITSQINKAKLPTHIEISSEDFSLPKDSVVLLEQIRTIDKRRLKEKIGRFDKNLMGE
                     VDDCLKISLGLIDF"
     gene            complement(5596..6906)
                     /locus_tag="PLD67_00175"
     CDS             complement(5596..6906)
                     /locus_tag="PLD67_00175"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOT20973.1"
                     /translation="MNYDEALQFIHSTYKFGSKLGLERIKRLMELLGNPQDCCKIIHV
                     AGTNGKGSTCNLIHDVLMESGYKTGLFISPYLEEFTERIQINKQKIDKDSLTRIAELV
                     RDKVNTMVSEGYDHPTEFEVVTAIGFKYFQEQNIDFLVLEVGMGGRFDATNVIKKPLL
                     SVITSISYDHMEQLGNTLEKIAFEKAGIIKENSTVIIYPQTINITNVIKDVAKEKNAK
                     VYEADKSNIEKTKSDITGQWFKYLKRDVFELPELKINLLGEHQLLNALTALSALEIIK
                     DKGYDISTKSIENGFSNCKHPGRFEIINKNPYVILDGGHNIDGIRSFTNTLKEYFKDK
                     VTFFFGILKDKSPEEALQLILPLAKEIYTLTPLSPRALKAADLAGLIKKYSDIKVIPL
                     ENYEDMLPIIKNAGKNEIIAFSGSLYMIGNVRTLLRNNGITNNL"
     gene            7275..9134
                     /locus_tag="PLD67_00180"
     CDS             7275..9134
                     /locus_tag="PLD67_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOT20974.1"
                     /translation="MIDVKKLDRVLLSVEKPARYVGSELNSVVKNKDIDIRFAFAFPD
                     IYEVGMSHTGLHILYHLMNSMENVWCERVFAPWTDMEEVMRKNNIPLYGLESKDDLTA
                     FDFVGFTLQYEMSYTNVLNMLDLGNIPIKSKDRGNDMPLVIAGGPCVYNPEPLHEIID
                     LFVVGEAEEVLPELLNLYNEEKKNVYDKLKFLEKAASIEGIYIPQFYEEIYNNDGTIK
                     ERIVLNPNAKETIKKRIIKDLSKSYYPEKSILPYIDVVHDRVVLELFRGCTRGCRFCQ
                     AGMVYRPVRERSTDTLINQAERLLSSTGHDEVSLTSLSSGDYSCLPDLTIRLLDKYEN
                     QNISISLPSLRLDSMTFDIIERLQEVRKSGLTFAPEAGTQRMRDVINKNLTEEQILGP
                     VETAFKLGWSTVKLYFMIGLPGETMEDVLGIKELSYKIKDKFFHRPKDEIKGNLKINV
                     SAACFVPKPFTPFQWAAQNNMDEFYEKAKVLQREIKDKKISFSYHEPKLSYLEAVFAR
                     GDRKLSKALIKAWEKGCRFDGWYDIFDFDKWMNAFEETNIDPDFFALRQREVREILPW
                     DFIDIGVKKEYLVSEYEKALDFDTTPDCRQRCTNCGVNYNLWGGACPHVQNNM"
     gene            9115..9789
                     /locus_tag="PLD67_00185"
     CDS             9115..9789
                     /locus_tag="PLD67_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HOT20975.1"
                     /translation="MCKIICKYSKTGYLKYISHLDVLRFIQRSVKRAGIKAKYSEGFN
                     PHMKTSFGYPLSLGIESIGEYFELELNENIEPELFKDKMNSVMPKEMQIIKADYSNDA
                     ESLMKRCAYARYLINIEPSIDVNKLNEYFEEMLTEGVIYKRQKINKKNKLVTKELNTK
                     ELIDYLKAENIDNKTRIMAVFKTQETGSMKVEEFIKLIIEKGFESDYYTIMKIDALDE
                     EMKPIL"
     gene            9805..11301
                     /locus_tag="PLD67_00190"
     CDS             9805..11301
                     /locus_tag="PLD67_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOT20976.1"
                     /translation="MKQVLLSNSEFFNQVAILQEGKLIDYLFELNNNQKVVGNIYKGK
                     VINILPGMQAAFVDIGLNKNAYLFLDDLLSDKFLKELKIKKKDVSNISKVLKTGDELL
                     VQVVREPMGEKNISVTTNISLTGKFIAFIPNNNVVNLSKKIKDPDERKRLEEIGKSII
                     LNGNGMIIRTFAKGSHKEAIEKEYKMLSNIYMKIEKEYKYSYAPKLLHKSNSLIDRLF
                     LDYIDSSVEEIYVEDKITKDKFNDMISGLPGDILKNIKIIQAQRVFENFNIASQINML
                     FERRVELDNGGFIIIDVTEALTVIDVNSGKYTGSRNMEETALAINLSAIEEIARQIKM
                     RNLSGIILIDFIDVKKEESINLLIAKAKSLFRDDKTKTNVIGMTKLCIMEITRKRNTE
                     NFFNLITEECSHCSGSGRSISKMYIFIKIEGIIKNIKHNTSSEAVILNVGCILYNKII
                     KSCMDIIAEIEKKYDIKIFIEQDDKILTDEIIIGKIGKLSNVNGIYQI"
     gene            11533..11844
                     /gene="rplU"
                     /locus_tag="PLD67_00195"
     CDS             11533..11844
                     /gene="rplU"
                     /locus_tag="PLD67_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOT20977.1"
                     /translation="MYAIIETGGKQYRVQEGDVLRVEKLDIAEGETVKFDKVLLVAEE
                     GKVNVGKPYVDGAVVEAVVENHGKAKKIIIFKYKSKKDYRKKQGHRQPYTQIKVEKIV
                     G"
     gene            11846..12172
                     /locus_tag="PLD67_00200"
     CDS             11846..12172
                     /locus_tag="PLD67_00200"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HOT20978.1"
                     /translation="MITVTIYRTKDEFKGFIVEGHSDYAEEGLDIVCASVSILSYTAL
                     NSLNLVAGITPENIEYTVDDEGVMSLRTLENNNKTDIVYRTFMVGIELLLEDYSDYIT
                     LKFEEV"
     gene            12175..12468
                     /gene="rpmA"
                     /locus_tag="PLD67_00205"
     CDS             12175..12468
                     /gene="rpmA"
                     /locus_tag="PLD67_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOT20979.1"
                     /translation="MLLKLNLQLFAHKKGVGSSRNGRDSNSKRLGVKRTDGQFVLAGN
                     ILVRQRGTKLHPGVNVGRGSDDTLFAMIDGKVKYERKDKTRKQVSVYPVELDA"
     gene            12560..13834
                     /gene="obgE"
                     /locus_tag="PLD67_00210"
     CDS             12560..13834
                     /gene="obgE"
                     /locus_tag="PLD67_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004825595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOT20980.1"
                     /translation="MFVDIAKISVKAGKGGNGSVAFRREKYIPMGGPAGGDGGDGGSV
                     ILVADEGLRTLMDFRYKKHYHAENGEDGRGKKQYGSAGEDLYLRVPVGTLVKDAETGI
                     VLADLKTHGQQYVAARGGRGGKGNVKFKSSIRRTPRFAQPGTKGDEREIILELKLLAD
                     VGLIGFPNVGKSTILAAITSAKPKIANYHFTTLKPNLGVVSIGEGHSYVLADIPGLIE
                     GASEGAGLGLDFLRHIERTKLLLHVIDASGQEGRDPVEDFHKINDELKQYSNKLSGKE
                     QIIVLNKMELPDAINNAERIKKEFSEKYEIIQVSAATGSGLDFLKKRAYERLLEIEEE
                     IEFVDEEAVESFLDKKERDTILVTKEDGYYSAEGDFLERLVASTNFDDFESFSNFQKV
                     LIDKGVIQKLKELGAEEGDLISVCGIEFDFVD"
     gene            13846..14136
                     /gene="yhbY"
                     /locus_tag="PLD67_00215"
     CDS             13846..14136
                     /gene="yhbY"
                     /locus_tag="PLD67_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HOT20981.1"
                     /translation="MLTGKQRSYLKALANSVDTIMQIGKGGITDSVLKQIDDALNARE
                     LIKVSVLKNSMLDAKSVANEVAESLGAEYVQSIGGKFVLYRESKEKKINIPK"
     gene            14475..15239
                     /locus_tag="PLD67_00220"
     CDS             14475..15239
                     /locus_tag="PLD67_00220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOT20982.1"
                     /translation="MIKGILFDKDGTLIDFSLWRNAGINTIQTILNEYNLNNNKLNIE
                     LQRAIGIKEKGVEPFGALAYKSHEDVAYELHCILNKYINIDFDKFETHVVGLLRNEVL
                     RDDVEFKEIVSIRKLYEHLNSNNIKMGLATADSMQSAMHMINKLNLNDSFDFIGSYDG
                     KMKRKPHKDMCMKFCSMYNLEPGEVAIVGDSYSDMLFALNSGAIGVGVLSGVSSKINL
                     KDVANVIVPSVESLFDRNVLEALDEKSYEARELRTA"
     gene            15341..15535
                     /locus_tag="PLD67_00225"
     CDS             15341..15535
                     /locus_tag="PLD67_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20983.1"
                     /translation="MNELEKIKKMYDNGFRCIRYDDTKDGDMCIYFKNFDNEESEALR
                     VSDFEQKMQIKSFIKENSMK"
     gene            15766..16593
                     /gene="thyA"
                     /locus_tag="PLD67_00230"
     CDS             15766..16593
                     /gene="thyA"
                     /locus_tag="PLD67_00230"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOT20984.1"
                     /translation="MSTADKIFKEMCKDILTKGVWDTDYDVRPRWEDGTPAHTIKKFG
                     IVNRYNLQESFPILTLRKTNFRAAVDELLWIWQKKSNNVNDLNSRIWDSWADETGSIG
                     KAYGYQLGIKHKYKEGEFDQVDRVLYDLKNNPASRRILTNIYNFQDLHEMHLYPCAYS
                     MTFNVSGNKLNAILNQRSQDVLAANNWNVVQYSILVHMMAQVSGLVAGELVHVIADAH
                     IYDKHVPIIEELIEMEEHEPPTLEIDNIDDFYKFTVDSFRLINYQYNPFKVKVPIAV"
     gene            16604..17125
                     /locus_tag="PLD67_00235"
     CDS             16604..17125
                     /locus_tag="PLD67_00235"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOT20985.1"
                     /translation="MKLIVAVARDWGIGNKGELLFNVPEDMEFFKKTTLNKVVVMGRK
                     TLLSLPGGTPLKNRTNIVMTSDVNFKKEGCIIVNSKEALFEELKKYESDDVFLIGGGI
                     LYKELYPYCSEAYITKFDAILEADTYLHNFDEDKDWLLTYASEIHEHNGVKFIFTIYK
                     NLTLNDKGTGVLS"
     gene            complement(17278..>17966)
                     /locus_tag="PLD67_00240"
     CDS             complement(17278..>17966)
                     /locus_tag="PLD67_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20986.1"
                     /translation="AEALRYLSNEDVEILSDAVNVESFSKLLMFDAKRIEHEERYGNY
                     IQITHTYEPGKKNMDSTTVYIELPKNVVYVMQYFKWKGGRPIWQDGILISGETAADDK
                     QIISSGLFYSKKGTEYTADFPRLICDRMTQHTMFGTYYPVLITGALSYPFGSEEQGGY
                     VLYRYTVMTDSDIYDTYNIFSYVHLSSPLHMPYTRTEDLILSGGYTFEDMLQQHQTNY
                     ESMALREKIK"
BASE COUNT         6157 a         2728 c         3593 g         5388 t
ORIGIN      
        1 caatgccccg gagttaggtt ttttgaatag ataaacagat aatttaatac agtagaatta
       61 caggaggaaa gaaaatgaaa agcttcttag ctaaacctaa tgaagttgaa agggcatggt
      121 atgtcataga tgccgaaggt aagacattag gtagacttgc aacagaagtt gccaaacttc
      181 ttagaggtaa acataaagct atatatacac cgcatgttga tacaggagac tttgtaatag
      241 tagtaaatgc tgaaaaggtt gtattgacag gtaaaaaatt agatcagaaa ttatacagac
      301 accactctct atatccgggt ggtttgaagg aagtacctta caggaattta ttccgaagta
      361 aaccaacaca gcctgtttat ctggcagtaa agggaatgct tccaaagaac agccttggaa
      421 gaaaaatgtt aaagaaatta aaagtatacg caggtcccga gcacaataat caggctcaaa
      481 aaccgcaagt atacgagttt taatgggaga ggaggaatac gatggaagca cagtacagag
      541 gaacaggcag aagaaaaaaa gcgatagcaa gagtaagatt agttcccggg acagggaaaa
      601 tcaacataaa tgaaagagat ttagatgatt atatcaaata tgaaacttta aggaaccttg
      661 taagagaacc tttaatgatt actgatacat tcggtcaata tgatgtactg gtaaatgtaa
      721 agggcggagg atatacaggt caggccggag caatcagaca tggtatatca agagcgcttt
      781 tacaggtaga caaggaatta agacccttgc taaaaaaagc cggattctta acaagagatt
      841 ccagaatgaa agaaagaaag aaatacggtt tgaaaaaagc aagaagagct tcacagttca
      901 gcaagagata aaaatatttg gggacgattc ttttttaaga aaacaaaaag gaccgtccct
      961 taaaaactta atctatagtg ttttcttggc acccacttaa aaaatcaagg ttttttagtg
     1021 actccgagaa tatattcgaa aggagttttt ttaatgaaaa gaaacaatcg gtttataaca
     1081 cgtactgctc ttatagcggc ggcttatgct gctttgacct atgcctttgc atggatgagc
     1141 tacgagcaga tacagtttag aatatcagaa gtattggtct tgtttgcatt tatagagcca
     1201 agatatggtc tcggcttagt attgggatgc atattggcta acttagcgag cccctttggg
     1261 gtaatcgaca ttgcggtagg ctcatttgca acatttttgg caatagtatt cattgtaaag
     1321 ataagaaaat tattcggatt gaataaaaaa gcgctgatta tagcaagttt gggacctgta
     1381 attacaaacg ctttactggt aggagcagaa ttaacttatt tgtttaacac tcccttttta
     1441 ttgaatgcat tatatgttgg agcaggcgag tttgcggtag ttgttttcat aggcactgtc
     1501 gttgtaaatt taataatgaa aaacaatgtc ctggttgaaa aactttcatt ctaaacgagg
     1561 acagtttctt tatccggtgc catttatccg gtgccaggga aacctccgaa aatatggagg
     1621 tttccctggc accgaatact tcaggcaccg aatacttgca ccggatgaga aaaaattgag
     1681 ctatccccaa atttacacaa atgaatgatt ttcgatttgt tgtgtttgca attgaacgcc
     1741 ttttggaatc caaaatttca aggcgttttt ttccgtcgat atatttaatt cattgtaagt
     1801 atatatttct ccatccatgc agacgttggt ttgtttatca aattttatat tagcttgatt
     1861 aatcttgtca tagataatat atttattgat tttaggatta tctaaatgct cacctttttt
     1921 gtaacttcct attatattta atattttagc tcttgagatt ttttccacaa agcataggtc
     1981 aagaagaccg tcatttactt ttgcttttgg agcacacttg tatcctccgc cgtagtattg
     2041 tccgtttgct ataacaccaa tcaaaaatgt tccgttgata acgccatttt ccgtattaac
     2101 ttcaatatgc tttcccaatt taccaaacaa agaatacagc atggaaagat agtatctggt
     2161 agtgccttga atgaaaggaa acttcctgaa tttatccatg ttgtaagctg catcggcatc
     2221 aagaccaata tttaatacat tggcagaata ttcattgtta accttaatta aatccagttc
     2281 tatgctttca ccttccgtct gcaggttaat atcgctgaaa ttagccgaat tcttgaaatt
     2341 cttaataaag tcattgccgg agcctatcgg tattatggca atactaacat gtttatacct
     2401 gcatgcgcca tttattattt catgaagggt cccgtcacct ccgcatgaat acaagacagc
     2461 ttgtatattt tctttgcatt tattttctgc aaagtttctt gtgtcttctt tagatttggt
     2521 gatatatacc tcataatcaa tttctttatc ttttaattca aggtgtatag ttttttataa
     2581 cttttcttgt aatttgccgt tgcctgcaat aggatttatt ataaatatgt gtttcatgtc
     2641 ctctccatct gtttgttatt gctatgaata acatttttgt cattctgagg gcttcagccc
     2701 gaagaatctc atgagatcct tcgcttacgc tcaggatgac aaaagggtgt tgtctgcaga
     2761 tccttcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngag atccttcgct
     2881 tacgctcagg atgacaaaag ggtgttgtct gcagatcctt cgtttcactc aggatgagcg
     2941 accaacacca aattaaaaat ttgggttgtc tgctcacttt gtgccgaata gtctgtctcc
     3001 tgcatctccc aaaccgggta cgatatatcc tatttcatta tatttttcat ctatggcagc
     3061 agtgtagatt tcaacatccg ggtgagtttc cgtaactctt ttcagtcctt cgggtgcgca
     3121 taccaaatga attgatttga ttttctttgc tcctcttttc tttagcatat tcaatgcacc
     3181 gttcattgaa cctccggttg acagcatggg ctctgtaata attactattc ttttatcaat
     3241 atctgatgga agcttgcaat aatattcaac gggctcaaga gtttcttcat ttctatacaa
     3301 accgatgtgg ccgattttag catttgggat taaatccaac ataccgtcca ccatgccgag
     3361 gcctgctctc agaacaggta caattgcaat gtctgctcca gatagaattt tacctgtagt
     3421 cttgcatatg ggtgtttcta tgtcaatatc tttcagtggc aggcttcgag ttacttcgta
     3481 tgccaagagc atggaaattt ctcttactaa ttctctaaaa tctttcgggg gtgtgttttt
     3541 gtctctcagc attgtcagct tgtgctgaat cagcggatgg gatactacgg taatattatt
     3601 catatgatac tccttattgt taaatgtaat ttaagaattg tattgtaact aaacatgtaa
     3661 tataattatt atatacaaaa agccctttgt caattaaaaa tttttaaagg atgagggaca
     3721 caccttcata atcaggtaaa ggaatgtccc tgtggaggaa gtatgattaa aggcactgga
     3781 atagacatta cggaagttga aagaataaaa aagaatatgg gaaatatccg gttccttgaa
     3841 aagatatatt caaacagaga attagaatat ttaaaaacaa ggaaattcaa tcctcaaaca
     3901 gctgccggaa tgtttgctgc aaaagaagct gttgccaaat gtttgggaac gggattttca
     3961 aatttcggtc cttgtcacat tgaaatatta aaggatgaaa atgataaacc atatgttttt
     4021 ttattaagca atgcattaaa aagagcagaa gagttaaata taaaaaacat atatatttca
     4081 atatctcaca cccaagagta tgccgtagca cattgcgtag ccgaatgatt tatggcatat
     4141 atttcaaaat ttcatcataa aatgaagtag gtatggggac acaccttcat agaaggatag
     4201 actgtgaaga aagtccatga aggcatatcc ccatacttaa aggatatatc tcaaagtccc
     4261 attcgattat gatggaagca ggttttggta tgagattaat aatatttatc ttagttttac
     4321 tgatggctct aagcggttgt actgcggagg ataaaccgtt tattctgcca gttaattatt
     4381 cagctaatgc attgatgaag gtatatacgg ataaaacgga aaatgattat aaaatcaata
     4441 ttatttgcag ggacggcaat tacagcatca ggacggaaac tgaaaatgaa gcatggaact
     4501 gtgcatatgt ttcagggaac agatgtatac taaacaatga taaatttcct gaaagcagcg
     4561 ttacaatcga aaattataaa ttaaatgata cattgctgca tgattttgat ttcgggaaat
     4621 ttgatgtatt tgaagacatt ccggaagaac tggtttattg ggatggtgaa tacaagcatg
     4681 tattaaattt tagcaaagac aatatgcttc caaagacaat ttttatttat aaatatgata
     4741 aattggtaaa agctatacag tatgaaaaaa taaatattga ggaataatat taataattat
     4801 ttacgaataa tacaaatgtt accatacaac ttatatgttt attgtgttaa aatataatat
     4861 aactgtgcaa ttgcgaggtg tgtatcttgg cggagaataa aaaagtactg atttccatcc
     4921 ctgcaaattt gttgagggaa ttggataatg cggtaaaaga agaaggaaca aacagaagtg
     4981 actttatcag gaaatccata aggttttatt tgattgaaaa aagaaagctt gaaatcagaa
     5041 acaagatgaa agcaggctat ttggaaatga gtaaaatcaa caagagcatt actgaagaat
     5101 actcagaagg ggattatgaa gactttttaa gctatgaaac taaattatta ggaagtgaat
     5161 agtttgattg ttaaaagggg agatgtatac tatgccgatt tgagccccgt aattggctcg
     5221 gaacagggag gggtaagacc ggtattagtt gtgcaaaatg atattgggaa tagatacagc
     5281 cctacagtga ttgtatctgc tattacgtct cagataaaca aggcgaagct gcctactcat
     5341 attgaaataa gttctgagga tttcagcctt cctaaggact ctgttgtttt gctcgaacaa
     5401 atcagaacaa tagataaaag aaggcttaaa gaaaaaattg gacgttttga taaaaacctc
     5461 atgggagagg tggatgattg tttaaaaatc agcttggggc tgatagactt ttagaagacc
     5521 taaccctgtt tttcagaatc ttgtctgaaa aacagggtta ggtttttctt ggcgaatttc
     5581 cagtttatgc gacttttaca ggttatttgt tataccatta tttcttaaca gtgtccttac
     5641 gtttcctatc atatataatg agcctgaaaa ggctattatt tcatttttgc ctgcgttttt
     5701 aataataggc agcatatctt cgtagttttc caaaggaatc acttttatat ccgaatactt
     5761 ttttattagt ccggctaagt ctgctgcttt taatgctcgc gggcttaaag gagttaaagt
     5821 ataaatttct tttgctaatg gcaatattaa ttgcaaagct tcttcgggag atttatcttt
     5881 taaaataccg aagaaaaatg ttactttgtc cttgaaatat tcctttaaag tgtttgtaaa
     5941 gctgcggatg ccgtcaatat tgtgcccccc gtcaagaata acataggggt ttttatttat
     6001 tatttcaaat ctgccgggat gtttacagtt tgaaaaacca ttttctatac tttttgtgct
     6061 tatgtcgtaa cctttatctt taataatttc caaagccgaa agtgcagtca gtgcgtttaa
     6121 aagctgatgt tctcccaata agtttatttt tagctccggt agctcaaata catctctttt
     6181 taaatattta aaccattgtc cagtaatatc ggattttgtt ttttcgatat tgcttttatc
     6241 agcttcatag acttttgcat ttttttcttt tgccacatcc tttataacat ttgttatatt
     6301 tattgtctga ggatagatta tgacagtgga attttccttt attatgcctg ctttttcaaa
     6361 ggctatcttt tccaatgtgt ttcccagctg ctccatgtgg tcatagctta tggaagtaat
     6421 aacgctgagc aagggttttt ttattacatt ggttgcatca aatcttcctc ccattccgac
     6481 ttcaagaacc aaaaaatcaa tattctgctc ctgaaagtat ttaaagccta tggcggttac
     6541 aacctcaaat tcggtaggat gatcatatcc ttccgaaacc attgtattaa ctttatctct
     6601 taccaattcc gcaattcttg tcagggaatc tttatcaatt ttctgcttat taatttgaat
     6661 tctttccgtg aactcttcta agtagggact aatgaataaa cctgtcttgt atcctgattc
     6721 catcagcaca tcatgaatta agttgcaggt tgatccttta ccgtttgttc ctgcaacatg
     6781 tatgattttg cagcagtcct gcgggttacc caataattcc ataagtcttt tgattctttc
     6841 aagaccgagt tttgagccga atttatatgt agaatgaata aattgcaatg cttcgtcgta
     6901 attcataata acctctctaa tataatttta ttaattttac aatacttttt tttctttttc
     6961 aatgtaattg tttaatacag tattgctttt ctataaaaat aataaaaata tacgctcacg
     7021 gtttcggcca cgaataattc tactagctca ttccggctaa aaatcctaca gcttgcaaac
     7081 tcgcttcgct caaacagtgc aagctgttta acggattttt aaccttcatt cgctaagaat
     7141 tatactcgtg tcctgcaaat gttcgcttaa tatttttatt attttttaat taagggagta
     7201 taataataaa agttgtcaac aaatgtttat tatgttaaaa tatcatagaa tttaaggaag
     7261 tcgaagggat taatatgata gatgtaaaaa aactcgacag agtgcttctt agtgttgaaa
     7321 aacctgctcg ttacgttgga agcgagctta actcggtggt taaaaataaa gatattgata
     7381 taagatttgc atttgcgttt ccggatatat atgaagtcgg aatgtctcat accggactgc
     7441 atattttgta tcatttaatg aacagtatgg aaaatgtatg gtgtgaaagg gtatttgcac
     7501 cttggacaga tatggaagaa gttatgagga aaaataacat tcctttgtat ggcttggaaa
     7561 gcaaggatga ccttactgcc tttgattttg ttggatttac tctccaatat gaaatgagct
     7621 acacaaatgt tttaaacatg cttgatttag gtaatatacc tataaaatca aaggacagag
     7681 gtaatgatat gcccttggta attgcaggag gtccttgtgt atataatccg gagcctctgc
     7741 atgaaataat agatttattc gttgttggtg aagctgaaga ggttttgcct gaactgttga
     7801 atttatataa cgaagaaaag aaaaatgtct atgataaact taaatttctt gaaaaggcgg
     7861 catcaataga aggtatatat attccgcaat tttatgaaga aatatataat aacgacggta
     7921 cgattaaaga aagaatagtg ttaaatccaa atgccaaaga aacaataaag aaacgtatta
     7981 ttaaggattt aagcaaaagc tattatcctg aaaaaagtat actgccctat attgatgtgg
     8041 ttcatgacag agtagttttg gaattattcc gtggctgtac gagaggctgc cgtttttgtc
     8101 aggccgggat ggtttacaga cctgtaaggg aaagaagtac cgatacgctg ataaatcaag
     8161 ccgagagact tctaagctca acaggtcatg atgaagtatc attaacttcc ctaagctccg
     8221 gagattattc atgtctaccg gatttaacta taaggctgtt agataagtac gaaaatcaaa
     8281 atataagtat ttctctgcct tcgttgaggc ttgattctat gacctttgat attatcgaaa
     8341 gactgcagga agttcgtaaa tccggcctta catttgcacc ggaagcagga actcaaagga
     8401 tgagagatgt tataaataag aatctgacgg aagaacaaat tttgggaccg gttgaaactg
     8461 cttttaaatt agggtggtca acggttaaat tgtatttcat gatagggctc cccggtgaaa
     8521 caatggaaga tgtgctcgga atcaaagagc tttcttataa aatcaaggat aaatttttcc
     8581 acagacctaa ggatgagata aaaggaaatt taaaaataaa tgtaagtgcc gcctgctttg
     8641 taccaaaacc ttttacaccc tttcagtggg ctgcacaaaa caatatggat gaattctatg
     8701 aaaaggcaaa agtattgcaa agagaaatta aagataaaaa aatttccttc agctaccatg
     8761 aacctaaatt aagttatttg gaagctgtat ttgcaagggg agacagaaaa ttatccaagg
     8821 ctttgattaa agcatgggaa aaaggatgcc gctttgacgg gtggtacgat atatttgatt
     8881 ttgacaagtg gatgaatgca tttgaagaaa caaacattga ccctgatttt tttgcattga
     8941 gacaaaggga agtaagagaa attttaccat gggattttat agacattgga gttaagaaag
     9001 aatacttagt cagcgaatat gaaaaagcat tagactttga tactactcct gactgcaggc
     9061 aaaggtgtac aaactgcggt gtaaactata atttatgggg aggtgcttgc cctcatgtgc
     9121 aaaataatat gtaaatacag caagacagga tatttaaaat atatttccca cttggatgtt
     9181 ttaagattta tccaaaggtc ggtaaaaagg gcaggcataa aggctaaata ttcggaagga
     9241 tttaatcctc atatgaaaac atcgtttgga taccccttgt cgctgggaat agaaagcatt
     9301 ggggagtatt ttgaattaga gcttaatgaa aatattgagc cggagttatt taaagacaaa
     9361 atgaactctg tgatgccaaa ggaaatgcag ataattaaag ccgattattc caatgatgcc
     9421 gaatctctta tgaaaaggtg cgcctatgcc cggtatttaa taaatattga gcctagtatt
     9481 gatgtaaata agcttaatga atattttgag gaaatgctca ctgaaggcgt aatatacaaa
     9541 agacagaaga taaacaaaaa aaacaaattg gtaaccaaag aattaaacac aaaggaattg
     9601 atagattatt taaaggccga aaatattgat aataaaacca ggataatggc tgtgtttaaa
     9661 actcaggaaa cagggagtat gaaggttgaa gaattcatta aattaataat tgaaaaagga
     9721 tttgaatcag attattacac aatcatgaaa attgatgcat tggatgagga aatgaagcca
     9781 attttataaa aacgggagag ggttatgaag caggttttgc taagcaattc ggaatttttt
     9841 aatcaagtgg caattttgca ggagggcaag cttattgatt atctttttga attaaacaat
     9901 aatcaaaaag ttgtaggcaa tatctataaa ggaaaggtta ttaatattct tcccggcatg
     9961 caagctgcct ttgttgacat aggccttaat aaaaatgcat atttgttttt ggatgacctg
    10021 ctttccgata agtttttaaa agaactgaaa attaagaaaa aagacgtttc aaatatcagc
    10081 aaagtgttaa aaacaggtga tgaactcttg gttcaggttg tgagagagcc aatgggggag
    10141 aaaaatattt ctgttacaac caatatttca ttgacaggaa aatttatagc atttattccc
    10201 aataataatg tagttaatct ttcaaagaaa atcaaagacc cggatgagag aaaaagactt
    10261 gaagaaatag gcaaaagtat aatcttaaac ggaaacggaa tgataataag aacattcgcc
    10321 aagggcagtc acaaagaagc aatagaaaag gaatataaga tgctttccaa tatatatatg
    10381 aagattgaaa aggaatataa atattcatat gcaccgaagc ttttgcataa aagcaattct
    10441 ctcattgaca ggttattttt ggactatata gactcaagtg tagaagaaat ctatgttgaa
    10501 gataaaatta caaaagataa atttaatgat atgatttcgg gcttgcccgg agatattctt
    10561 aaaaatatta aaattataca ggcacaaagg gtttttgaaa atttcaatat tgcaagccag
    10621 ataaatatgc tatttgaaag aagagttgag cttgataacg gaggcttcat aataattgat
    10681 gttacggaag cattgacggt aatcgatgta aacagcggga aatataccgg aagccgaaat
    10741 atggaagaaa cagctttggc tataaaccta tctgcaatcg aagaaattgc aaggcagata
    10801 aagatgagaa atttaagcgg aattatttta attgatttca ttgatgttaa gaaggaagaa
    10861 agtattaatc tgctaatagc taaagccaag tcattgttta gggatgataa aacaaagaca
    10921 aatgttatcg gaatgacaaa gctatgtatt atggaaataa caaggaaaag aaacacggaa
    10981 aactttttta atctgataac cgaagagtgc tcacactgca gcggaagcgg cagaagcatt
    11041 tcaaaaatgt atatatttat taaaattgaa ggaataataa agaatattaa gcataataca
    11101 tcttccgaag ctgttatttt aaatgtggga tgtatattgt acaataaaat aatcaaaagc
    11161 tgtatggata ttattgctga aattgaaaaa aaatacgata ttaaaatatt tattgagcag
    11221 gatgataaaa ttcttaccga tgaaattatc atcgggaaaa taggaaagtt gtccaatgta
    11281 aatggaatat atcaaatata gcagtttcgc aggctccatc accattttgc tcactccctg
    11341 tggtcgtgca aaatgggaat gtgctgcgtc tgacctacca tccatttttc agaaaaatgg
    11401 ggttaggtca tcaaaaaata agttgacaat ttttttatag tttgatacaa tcaatatctg
    11461 ttagagccgc acggatcagg ttgaaaacgc taagtacctg caatggcgag actgggaaga
    11521 ggaggtaaaa atatgtacgc aattattgaa acaggtggaa aacaatatag agttcaagaa
    11581 ggcgatgtgt taagagttga aaaacttgat atcgctgaag gtgaaaccgt aaaatttgat
    11641 aaggtattgc ttgtagccga agaaggaaaa gtgaatgtag gcaaaccata tgttgacggt
    11701 gcagtagttg aagcagtagt tgaaaaccac ggcaaagcta aaaaaataat aattttcaaa
    11761 tacaagtcta aaaaagacta caggaaaaag caaggtcatc gtcagccata tactcaaatt
    11821 aaggttgaga agatagtagg atagtatgat tacagttacc atatatagaa ccaaggatga
    11881 attcaaaggt tttatagtag aaggccattc ggattacgct gaagaaggat tggacatagt
    11941 atgcgcatca gtttccattt tgtcttacac agcattgaat tcattgaacc ttgtggcggg
    12001 aattacccct gaaaacattg aatataccgt cgatgatgag ggagtaatga gtttgaggac
    12061 attagagaat aataacaaaa ctgatatagt gtacagaacc ttcatggttg gaattgaatt
    12121 attattggaa gattacagcg attatattac acttaaattc gaggaggtgt aaaaatgtta
    12181 ttgaaattaa acttacagtt atttgcacat aaaaaaggtg ttggtagctc aagaaacggc
    12241 agggacagca attctaagag attgggagta aaaagaaccg acggtcaatt cgtgttggcc
    12301 gggaatattc ttgttagaca aagaggtact aaacttcatc cgggagttaa cgtaggcaga
    12361 ggaagtgatg atacgctatt tgccatgatt gacggaaagg ttaagtacga aagaaaagat
    12421 aaaacaagaa aacaggtcag tgtatatcct gttgaattag atgcataata aaagaaaaga
    12481 cctaccatac ggtaggtcat ttttagatga agaaatataa agattgtcag gatgacaaga
    12541 gtggcggaaa ggaacaaaaa tgtttgtaga tatagcaaaa ataagtgtaa aagcaggtaa
    12601 gggcggaaac ggaagcgtag cattcagacg tgaaaaatac ataccaatgg gcggacctgc
    12661 aggcggcgac gggggcgacg gcggaagcgt cattttggtt gccgatgaag ggcttagaac
    12721 attgatggat ttcagatata aaaaacatta tcatgcggaa aacggtgaag acggaagagg
    12781 gaaaaaacaa tatggttctg ccggggagga cctttatctg agagttcctg tcggaacttt
    12841 ggtaaaagat gcagaaacag gcatagtact ggcagactta aaaacccacg gacagcaata
    12901 tgtagcagca agaggcggac gcggaggaaa aggaaatgtt aaatttaaaa gttccataag
    12961 gcgtactccc cgttttgcac agcctggaac caagggcgat gaaagagaaa ttattcttga
    13021 gcttaagctt cttgccgatg tcggattaat agggtttcca aacgtgggga aatcaacaat
    13081 attagcagct ataacaagcg ctaaaccgaa aatagcaaat tatcatttca cgactttaaa
    13141 accgaatctt ggagttgtga gcataggaga aggacacagc tatgtgcttg cagatattcc
    13201 ggggcttatt gaaggtgctt ctgaaggtgc aggtctcggg cttgattttt taaggcatat
    13261 tgaaagaaca aagctgcttt tgcatgttat cgatgcttcc ggtcaagagg gaagagaccc
    13321 ggttgaagat ttccataaaa taaatgatga gcttaaacaa tacagcaata aactatccgg
    13381 aaaagaacaa ataattgttt taaacaaaat ggagctgccg gatgctataa ataatgccga
    13441 aagaattaaa aaagaatttt cagaaaaata tgaaataatt caagtttctg ccgcaacagg
    13501 cagcggactt gactttctta agaaaagagc ctatgaaaga cttttagaga ttgaagaaga
    13561 aattgaattt gttgatgaag aagctgttga aagtttctta gacaaaaaag aaagagatac
    13621 aattcttgta acaaaagaag acggttatta ttcagcagaa ggagattttc ttgaaagact
    13681 tgttgcatca acaaattttg atgattttga atctttttcg aattttcaaa aggtactgat
    13741 tgacaagggt gtaatacaga aattaaaaga actcggcgct gaagaaggag acttaatcag
    13801 cgtatgcgga atagaatttg attttgtaga ttgaaaggag agtaaatgct taccggaaaa
    13861 caaagaagtt atttaaaagc tttggcaaat tctgttgata caataatgca gataggcaaa
    13921 ggcggtatta ctgacagcgt attaaaacaa attgacgatg cacttaatgc cagagaatta
    13981 atcaaggtga gtgtattaaa aaacagcatg ctggatgcaa agtctgttgc aaatgaagtt
    14041 gcagaatctc taggtgctga atatgttcaa agtataggcg gcaaatttgt tttatacaga
    14101 gaatctaaag aaaagaaaat taatatacct aagtaaaagt caggtgccgg aatgcaatca
    14161 ttcatgcacc tgattcgtca gtgtcattct gaacgcatat gaagaatctt atgaaatcct
    14221 tcgctcggga aaacaaagcg cattcattta tcgtggtgta gataatacgt gatgattctt
    14281 atcatatttg tttgaatttc aaatttatat cacatttcag ctttttctta atataatagt
    14341 actccccgca atttaagaat gtttaaagaa tcatattata aaataaaaaa gatgcactta
    14401 tttatacgta ttacaatatg agcatatcat atttattttc gtatttacaa tatgaaatat
    14461 aaggaggtga taaaatgata aaaggaatac tgtttgataa ggacggcacc ttgatagatt
    14521 tttcattgtg gagaaatgcc ggaatcaata caattcaaac aatactaaat gaatataatt
    14581 tgaataataa caaacttaat atagagctgc aaagagctat cggcataaag gaaaagggag
    14641 tggagccttt tggtgccttg gcttataaat cgcatgaaga tgtggcttat gagcttcatt
    14701 gcatactgaa taagtatata aatatagact ttgacaaatt tgaaactcat gtggtcggac
    14761 tattgagaaa tgaagtttta agggatgatg ttgagtttaa agaaattgtc agcataagaa
    14821 aattgtatga acatttgaat tcaaacaata taaaaatggg tttggcaacg gcagattcca
    14881 tgcaatcagc aatgcatatg attaataagc tgaacttaaa tgacagcttc gactttatag
    14941 gctcctatga cggtaaaatg aaaagaaaac ctcacaagga tatgtgcatg aaattttgca
    15001 gcatgtataa tttggagccc ggagaggttg caatagtggg ggattcatat agcgacatgc
    15061 tctttgcatt aaattcaggt gcaatcggag taggggtact gtccggagta agcagtaaaa
    15121 ttaatttaaa agatgtagcc aacgttatag ttccttctgt tgaatctttg tttgacagaa
    15181 atgtgctaga agccttagat gaaaaaagtt atgaagcaag ggagcttcga acagcataag
    15241 cctgtgcaag aaatgcaata aggcttattt ttttctttaa ttcagtcata atttccttta
    15301 tttggtcaat aatatgtata aaacataagg ggtgaatatt atgaacgaat tagagaagat
    15361 taaaaaaatg tacgataatg gattcaggtg cataaggtat gatgatacta aggacggcga
    15421 tatgtgcata tatttcaaaa atttcgataa cgaagaatca gaagccttaa gagtttcgga
    15481 ttttgaacaa aaaatgcaga taaaaagttt tatcaaagaa aattccatga aataagaggg
    15541 aacttaagtt tacagttcct atttatgtcc aattatttct tgtaaaacga ttccttctat
    15601 tatataatga atgcaaacaa tgaagcaaaa gctttggctt ttgcactgat gcattcattg
    15661 tatcggtgtg aagcaagccg attttgcggc ttgcactgat gataaaataa atgcaaacaa
    15721 tgaagcaaaa gctttggcta taatatataa tgcgtgggag gaattatgag tactgcagat
    15781 aaaattttta aagagatgtg taaagatata ttgactaagg gagtatggga tactgattac
    15841 gatgtaagac caagatggga ggacggcacg cctgctcaca ctattaagaa gtttggcata
    15901 gtaaacagat acaatttaca ggaaagtttt cctattctaa cactgagaaa aacaaatttc
    15961 agagctgcag ttgatgaact tttgtggata tggcagaaaa aatcaaataa cgtaaatgat
    16021 ttaaacagcc gtatttggga ttcctgggca gacgaaacag gctccattgg caaggcttac
    16081 ggttaccaac tcggaataaa acataaatac aaggaaggcg aattcgacca ggtagacagg
    16141 gttttatatg atttaaaaaa taatccggca agcagaagaa ttttaacaaa tatttataat
    16201 ttccaagacc tgcatgaaat gcacttatat ccatgcgcat acagcatgac tttcaatgtt
    16261 tccggaaaca agttaaatgc catattaaat cagcgctctc aggatgtctt ggcagccaac
    16321 aactggaatg tggtgcaata ttcaatattg gtacatatga tggctcaggt aagcggactg
    16381 gttgcaggag aattagttca tgtaattgcc gatgctcata tttatgacaa gcatgttccc
    16441 ataatagaag agcttattga aatggaagag catgagccgc cgacattgga aatagataat
    16501 atagatgatt tttataaatt tactgtcgat agttttaggt tgattaatta ccaatacaat
    16561 ccttttaaag taaaggtacc gatagcggta taaaggagaa accatgaaat taatagttgc
    16621 ggtagcaaga gattggggaa taggaaataa gggagagctc ttgttcaatg tgcctgaaga
    16681 tatggaattt tttaaaaaaa ctacattgaa caaggtcgtc gttatgggca gaaaaacact
    16741 tctttctctt cccggcggga cccccttaaa aaacagaaca aatatagtta tgacaagtga
    16801 tgtgaatttt aaaaaggaag gttgcatcat agttaattca aaagaagctt tgtttgaaga
    16861 gttaaaaaaa tacgagagcg acgatgtgtt tttaataggt ggaggtatat tgtacaagga
    16921 gctttatccc tattgcagtg aagcttatat taccaaattt gacgccatat tggaagcgga
    16981 tacatatttg cataatttcg atgaagacaa ggattggctt ctcacttatg catcagaaat
    17041 acatgaacac aacggagtaa aatttatttt caccatatac aaaaacttaa ccttaaatga
    17101 caaggggacg ggggtactgt cataatttga taatactccg tccccttgac ccggagggga
    17161 cggttctctc cggacaatta aactaaatga catcaatgaa accatcctcg gtttaaaata
    17221 agtgctgaaa tatctttcta tcaacccacc ttcataagaa ctaaaccatt cccaaaacta
    17281 tttgattttt tctcttaatg ccatagattc ataatttgtt tgatgttgct gtaacatatc
    17341 ctcaaaggta taaccaccac ttaaaatcaa gtcctctgtc cttgtgtatg gcatgtgtaa
    17401 tgggcttgaa aggtgtacgt aactaaagat attataagta tcataaatat cactgtcagt
    17461 catgacggta tagcgataaa gcacatagcc gccctgctct tcagagccaa agggataact
    17521 tagagcacct gttatcagta cggggtagta ggtgccaaac attgtgtgtt gtgtcattct
    17581 gtcacatata aggcggggaa aatccgcagt atactctgtg ccttttttgc tgtaaaagag
    17641 accgctgctg attatctgtt tatcatccgc cgctgtttct cccgatatca gaattccatc
    17701 ctgccagatt ggccgtccac ccttccattt gaagtattgc attacatata ccacattttt
    17761 cggtaactcg atgtagaccg tagtagaatc catatttttt ttacccggtt cataagtatg
    17821 agttatttga atgtagtttc catatctttc ctcatgctca attctctttg catcaaacat
    17881 aagcaactta ctgaatgatt ccacattcac agcatcactt agaatttcta cgtcttcatt
    17941 actgagatac cgcagagctt cagccg
//
LOCUS       DAOSOW010000003        16051 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_3677_length_16051_cov_3.747312, whole genome shotgun sequence.
ACCESSION   DAOSOW010000003 DAOSOW010000000
VERSION     DAOSOW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 16051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16051
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3677_length_16051_cov_3.747312"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLD67_00245"
     CDS             <1..447
                     /locus_tag="PLD67_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HOT20987.1"
                     /translation="LSAAARRNDTTLIAVVLGCDNTKIRFDEVTKLLNEGFANYKNIV
                     FHKAGDTITTSPVYCGKEETINIVSKENINYFTESNCKPEDFNLEYVIDENLKAPLNV
                     DKKIGRAILSKDGRVLGEFELYPGANVEKENLFKFFINNFLKTTIR"
     gene            460..1188
                     /locus_tag="PLD67_00250"
     CDS             460..1188
                     /locus_tag="PLD67_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HOT20988.1"
                     /translation="MDYSVNINVFQGPFDLLFHLIEKKEIDIYDIPISEITDQYLEYL
                     EEMMQFNMNVASEFIFMAATLVEIKSQMLLPQKEKEEDPRQELVNKLLEYKMYKDLSE
                     ELKKYEDESSFYISKPREEMAITSDTKTEQLSLNEINVYELYNIYLSLIKKQNFRIEP
                     EEKFKIYREKYSVKGCMEDIVKKIKKFGKVSLFNTLKEKENISKEYIVTLFLAILELS
                     NNKGMKIYQENIYGDIIITKQERK"
     gene            1185..1709
                     /gene="scpB"
                     /locus_tag="PLD67_00255"
     CDS             1185..1709
                     /gene="scpB"
                     /locus_tag="PLD67_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HOT20989.1"
                     /translation="MNIKSVLESLLFAWGEPLSIIEMSKILEKPADSIILVLEEMISE
                     FNDNEDRGLIIQKFGDSYQLTTKKANYEYIQKLLETTVSKSLSTAALETLSIIAYKQP
                     VTKVDIERIRGVKCSQVVKGLIDKGLIKEVGRLDKPGRPVIFATTDEFLRHFGLSSID
                     ELPSIGDIASTVEV"
     gene            1786..2157
                     /locus_tag="PLD67_00260"
     CDS             1786..2157
                     /locus_tag="PLD67_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20990.1"
                     /translation="MGILIVLLGGLLFLYLNIKCAFKINITFYFLHVYFYVIIFRKKH
                     EYQKRINFSAKKIIDRYKAPELRQKTKNNLKYLKHIKKVFNIFYVKDIFFYPECILEK
                     QSFALEFVIVNRMLKKSLLNG"
     gene            2303..2683
                     /locus_tag="PLD67_00265"
     CDS             2303..2683
                     /locus_tag="PLD67_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT20991.1"
                     /translation="MSKDIESIIKTTLDNINNLTENEKLISSKICYDNTNYLAISKLN
                     MNFIIGGSDIDKKFRTVDLKPFAGAAYVNLQLNPQILIYETPDNNIKTININNETTAG
                     DITSSIMNIMSFIKEIREKKDCDI"
     gene            2673..3704
                     /locus_tag="PLD67_00270"
     CDS             2673..3704
                     /locus_tag="PLD67_00270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOT20992.1"
                     /translation="MIFKRAIAILTVIFIFTCEFTCVEAMPGVSAQAYIVIDAQSGRI
                     LKSHNENKRLPIASTTKIMTAILAIETIDDLNQRIEVPPECSGIEGSSIYLRPKQMVS
                     VIDLLYGTMLRSGNDAAETLSKFAGRNNPEGFISMMNKKAKELGALNTNFENANGLHD
                     DNHYSTAYDLALISAYAMKNDTFRKIVSTEKYKAESMNNMFYNKNKVVYQYKYGTGIK
                     IGFTKAAGRCIVASAEKDGTEIIAVLLNDNNWFNDSYQIFDWAFENYKCYSIVEKGQF
                     VKYTPERNPVFIGESFRCVLTDKEIKQIRFEALIKPELMSNDSETKTCGTYNIYLGKT
                     KLGTGYLIR"
     gene            3856..4563
                     /locus_tag="PLD67_00275"
     CDS             3856..4563
                     /locus_tag="PLD67_00275"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017753298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOT20993.1"
                     /translation="MEERLQKYIARCGVASRRKAEEIILQGNVKVNGIIIKELGSKIN
                     PERDIVTVNNQRIYEKQKNIYIKMYKPEGYITTVKDQFNRKTVLDLINIKERIYPVGR
                     LDYDTSGLLLLTDDGDLANKLMHPKYRIFKTYKAEIEGGISEDSINKLRNGVLIDGYK
                     TAPAKVKLLKKTQKNSFVQISIHEGKNRQVRKMFEKVGHRVIKLKRLSFGNINLDGLR
                     EGQWKYLTDDEIEYLKR"
     gene            4566..5138
                     /locus_tag="PLD67_00280"
     CDS             4566..5138
                     /locus_tag="PLD67_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT20994.1"
                     /translation="MDKMYGQITEMVHFLIKGSYEGKEDLRFVDLTCGNGYDTLFLSN
                     LAGKKGFVTAFDIQETAIERTKKLLQEESNFNNYKVIHNGHEHIDKYLTEKIDAAIYN
                     LGYLPNFNKDIFTKPETTICSINSLFPYLKNSGRIYITAYTSHDKGCEINSIYDYLIK
                     LDKKEYNVIHIKVINKNNTPPELFIIEKNS"
     gene            5368..5565
                     /locus_tag="PLD67_00285"
     CDS             5368..5565
                     /locus_tag="PLD67_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT20995.1"
                     /translation="MAEEKILLQNIPWCKGCGVCVGFCPVKVLEIKDDKCVIAHRDKC
                     IFCGQCELRCPDNAIWIGGKK"
     gene            5562..6698
                     /locus_tag="PLD67_00290"
     CDS             5562..6698
                     /locus_tag="PLD67_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOT20996.1"
                     /translation="MSNKVYKLVQGNEACVEGALAAGMRFFAGYPITPSTEIAEISAE
                     KLPALGGRFIQMEDELASMAAIIGGSLAGKKSMTATSGPGFSLMQENIGYAAITEIPC
                     VVVNVQRGGPSTGLPTSPAQGDVMQAKWGTHGDHPAIALTPNSVQETYEYTVKAFNLA
                     EKYRTPVILLMDETVGHMREKMAFKDVSEIEIIDRKKPTCKTEDYVAYRPDEDMIPPM
                     ASFGEGYRYHVSGLTHDETGFPTNNNEISEKLVKRLVDKVNKNLDDFSYYEEYKLEDA
                     EIVIIAYGGVSRSAKSAVDELRKQNIKAGLFRPITIWPFLEKQVNKVSLNAKEIYVAE
                     MNLGQYFLEVERAACKNCTVKNIGKVNGELITPEEIVQAIGGNK"
     gene            6698..7513
                     /locus_tag="PLD67_00295"
     CDS             6698..7513
                     /locus_tag="PLD67_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOT20997.1"
                     /translation="MNCNEITKKYYRANKLPHIWCPGCGHGTLTNTIVRAIDKLGLNK
                     DEVVIVSGIGCSSRAPGYLDFNTLHTTHGRALAFATGIKFANPNLHVIVIMGDGDSSA
                     IGGNHLIHAARRNIDITAVVFNNNIYGMTGGQFSPTTPSGDMATTTPHGNIDKPFDLC
                     SLAQGAGATYVARGTAYHAVLMQKLIENGIQNKGFSFIEGLSLCPTYYGRKNKKGSAF
                     KMHTFLKENCVDKKVVDKDPEKGKNKILIGEFYNNPQPEYTESYQAVIDKLQG"
     gene            7524..8060
                     /locus_tag="PLD67_00300"
     CDS             7524..8060
                     /locus_tag="PLD67_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOT20998.1"
                     /translation="MKKLEIRLSGSGGQGVILAAIILANAGVEQGLNAIQTQSYGPEA
                     RGGASKAEVILSNGEINYPKISTANILLSLTQKAYDKYKSSLEKDGILIVDESVKLRG
                     DETFKVYSLPILRTALEKFETHMVANIISLGAIYELMGENIIDKDIMIRSITERVPAH
                     TVQKNTDAFEEGIKLARG"
     gene            8062..8916
                     /locus_tag="PLD67_00305"
     CDS             8062..8916
                     /locus_tag="PLD67_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HOT20999.1"
                     /translation="MNDLISIIKKNFHKFSRGQKLIAQFIIDHYDKAAFMTAAKIAET
                     VDVSESTVVRFASALGYSGYPEMLKALQVLIKNKLTTVQRISLNDDVNDKLKLHKRNL
                     KNEMNNLRYLYDHFDMEALDKATELILEADRVFVLGLRTSSTLSNYLGFYLDVILNNA
                     KVLNNSGVNSLYEEIIRIKEDDLLIIISYPRYSRTTLDAARFVKERNTKIVAITDTEE
                     SPAYELADVSLLSKSNIVSFVDSLVVPMAMINQLIIDISLREKEEIVEYFNTLERLWD
                     HNSIYQNF"
     gene            9250..9900
                     /gene="cmk"
                     /locus_tag="PLD67_00310"
     CDS             9250..9900
                     /gene="cmk"
                     /locus_tag="PLD67_00310"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOT21000.1"
                     /translation="MIIAIDGPSGAGKSTVARLLSKELGYEYIDTGAMYRALAYKAYK
                     QNIDINEANIAELLETTNITYYDNKVFLDGENVENLIRNEEISIAASKISSLKIVREK
                     MVEIQRKIAKNKNVVLEGRDIGTIVFPDAEHKFFITASLEERAKRRYEQLKLNNIKAD
                     YTNVINDMIKRDENDSTRKFSPLKPAEDAILIDTTNMDLNEVTKTIAQYIGGSNVL"
     gene            9890..10486
                     /locus_tag="PLD67_00315"
     CDS             9890..10486
                     /locus_tag="PLD67_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOT21001.1"
                     /translation="MFYKIAVIVLKVLVFIIFDLEVYNRENLDNIDGGLIICGNHRSM
                     IDPVILAVSTKRQIHFMGKKELFENKFLSFLFRSLGAFPVDRKGVSLSAIKNSLNVLN
                     KGGILGIYPEGTRIKTGFDEKNAKSGIALIANKAKVGIMPVYLDGPYKFRGKLRLYIG
                     EEKDYFKNYSGRLNSEDYTEIGIEILKDIYRLKNEGMK"
     gene            10483..12897
                     /locus_tag="PLD67_00320"
     CDS             10483..12897
                     /locus_tag="PLD67_00320"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF000907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HOT21002.1"
                     /translation="MKIIVSEYNGFCGGVKAAVLKADKILTDEKKLYCYGEIIHNKDV
                     VEKLKNKGMVIVDDIPENKDAKFLIRSHGVGKNIYDRLEEKNIEVIDATCVKVKKIHK
                     IVEEFKNKDYNIIIVGNENHPEVQGISGWCNYEAVIIKSPEELINIIEPTKKYCLVCQ
                     TTFNLNTFEEILKTIEINNYSNIIVHNTICDATRKRQEACIALASKCDIMLIVGGKNS
                     SNTKKLYELSKELCENTFLIQNYKEIPYNYIDKNSIVGVSAGASAPDWIIEEVINMLE
                     NLNNNMNEMNEQAEHADLAEQSEPAELSDKKEQTELVEQSEETEKTETVEQTDKAEKV
                     NSMHELLENYGGVVNLGIGEKVKGTVIYVSPDAVSVNINYKSDGIITREDFSLEEVSD
                     LTEVVKEGDEIDAIVVKISDQDGNVVLTRKPLEEQKIWDDLEQMRIRGEIVEIPIIEA
                     SQHGIYGKLKGIKGFIPRNQISIKRNVDPSEYVGKNLKVKILETKNKKGRRQLVLTSR
                     AVEKFEKEIKDNEVWETIQEGEIYEGTVKNLQDYGAFVEINGIDGLLHINEIAWTRIK
                     HPSEVLKPGDKINVKVKNVDKENKRLSLSYKATVKSPWAKFLDKYQKGDIVTGVVTRI
                     VDFGAFVKVDDIECLLHIKDLDWARTEKVTDILQEGQEVTAKILSITRKDRKVGLGLK
                     QLTEHPFDLYAKTLNKDDIIQVEVTRILLDGIHVNANGNIDCFIPIAKVSTEKLRTPA
                     QVVKVGEIVDAKVQSIDMKGKNLNLTLILDDKSEETVENKSYQSEDTNFTIGESIGDA
                     LDDLLK"
     gene            12952..13992
                     /locus_tag="PLD67_00325"
     CDS             12952..13992
                     /locus_tag="PLD67_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOT21003.1"
                     /translation="MLKEFLFDICSNQFVSGFEHINGDILIKYFKPYTDSFEKDRLGS
                     YIFKSSGSKDTKIMLAAHIDEIGLMITGILDGGFLSFITVGGFNPASLIAQDVTIFGK
                     EKIFGVIGIKPPHLTSEEERKKPLKTRELYIDTGYTKEKLEKLITIGDIATINREPLS
                     LQGSVISAKAFDDRACVAVMLETAKELKKISHKSNVYFTATAQEETGLRGAKTSSYKI
                     APDIGIVLDVGFGKTPDMPSDSALLGKGPIVAYGGRLNTKLTKKFVEICKKYNYPMQY
                     EAIPVRTGTDTDALQISRDGIPCILLSIPLRYMHTSVETIDLKDLEITGKAIARFINE
                     LDNSDLEEILCF"
     gene            13983..14996
                     /locus_tag="PLD67_00330"
     CDS             13983..14996
                     /locus_tag="PLD67_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOT21004.1"
                     /translation="MFLNELTKLSGVSGCEFEVRNYIKSKLSEMGCQFYVDKLGNIIA
                     HNKGKKNKTIMVSAHMDEVGLIVTNIDSDGFIKFKAVGGIDQRVLNSKVVLIGENNIP
                     GVIGSKAVHLMTEKEKGVSLPIDKLYIDIGTSTKEETEKLVNIGDYAAFKSDYVEFGD
                     NMIKAKALDNRVGCSIILEILKEKLDVDFYGVFTVMEEVGLRGAETAAYQLEPDLAIV
                     LEGTVCADMPEVEEHENSTLIGKGAVLSLMDNATVYDIDLVRRVAKIAEDNNIAYQYR
                     RSTSGANDAGVIHKTKKGAKVVSISVPCRYIHSSVSVASKNDLMSALKLTKQVIIENQ
                     KGE"
     gene            14998..15981
                     /locus_tag="PLD67_00335"
     CDS             14998..15981
                     /locus_tag="PLD67_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOT21005.1"
                     /translation="MFNSELMKRLSDCFGPSGREKRIREMIMDEIKDFADEITIDPLG
                     NLIARKKGTGKKILFSAHMDQIGLIITHVDEKGLLRFANVGGLNAKELLGLRMVFDNG
                     LEGVICQEELKDNEKLSTSKLFLDVASTDKEFIAKNFRVGDMCVFKSEYYETEDCVIC
                     KAADDRIGCYVLIEALKNHPETNNDVYYVFSVQEEVGCRGAKTAGYNINPDLAIAVDV
                     TATGDTPDGIKMDVKLGNGAAIKLMDKSIITHTEVKDLLTDIAEKNSIKYQYEILEFG
                     GTDAGPIHTSRDGVPSGVISIPTRNLHTSGEIFNKFDVEECIKLVIGTAAK"
BASE COUNT         6221 a         2287 c         3134 g         4409 t
ORIGIN      
        1 ttatcggcag ctgcaagaag aaatgacact acattaattg ccgtagtcct cgggtgtgat
       61 aacactaaaa ttcgtttcga tgaagttaca aaactattga atgaaggatt tgcaaactac
      121 aaaaacattg ttttccataa agcaggtgac acaataacca catcaccggt ttattgcgga
      181 aaagaagaaa cgattaatat agtttcaaag gaaaatatca actattttac agaaagtaat
      241 tgcaagcctg aggattttaa tttagaatat gtaatagatg aaaatctaaa agctccttta
      301 aacgtggata aaaaaatcgg aagagccata ctatctaagg atggtcgggt attaggcgaa
      361 tttgaattgt atccgggagc aaatgttgaa aaagaaaatt tatttaaatt ttttatcaat
      421 aactttttaa aaactaccat aagataagga aggctggaaa tggattactc cgtaaatata
      481 aacgtctttc agggaccttt tgatctcttg tttcatctaa tcgaaaaaaa ggaaattgat
      541 atttatgata tacccatatc tgaaataaca gatcaatact tagagtacct cgaagaaatg
      601 atgcaattca atatgaatgt tgcaagcgaa tttatattta tggcagcaac cttagttgaa
      661 ataaaatctc aaatgctttt acctcagaag gaaaaagaag aagatcctag gcaggaatta
      721 gttaataaat tactcgaata caaaatgtat aaagatttat cggaagaact gaaaaaatat
      781 gaggacgaga gcagttttta tatttccaaa ccaagagagg agatggctat aacttcagat
      841 acaaagacag agcagctttc actgaatgaa ataaatgtat atgaacttta caatatatat
      901 ttatctttga ttaaaaaaca gaattttaga attgaacctg aagaaaaatt caaaatatac
      961 agggaaaaat acagtgtaaa gggctgtatg gaggatattg taaaaaaaat taagaaattt
     1021 ggaaaagttt cattattcaa taccttaaaa gaaaaagaaa atatatcaaa agaatatatt
     1081 gtaacattgt ttttagccat attggaactt tcaaacaata aaggaatgaa aatatatcag
     1141 gagaatattt acggagatat aataatcacc aaacaggaga gaaaatgaat ataaagagtg
     1201 ttttggaaag tttgctgttc gcttgggggg agcccttaag cataattgaa atgtcaaaaa
     1261 tattagaaaa gcctgctgac agcatcatat tagtattgga agaaatgata agcgaattca
     1321 acgacaatga agacaggggt cttattattc aaaagtttgg ggattcttat cagctcacta
     1381 ccaaaaaggc taattatgaa tatattcaga aactattgga aactacggta tctaagagtt
     1441 tgtcaacagc tgcattggaa acactgtcca taattgcata caagcagcct gttacaaagg
     1501 tggatataga aagaataaga ggggttaagt gctcccaggt ggtaaaaggg cttattgata
     1561 aaggattgat aaaagaagtg ggacgtcttg ataagcccgg cagacccgta atttttgcta
     1621 caacagatga attcttaaga catttcggct taagctcaat tgatgaattg ccgagtatcg
     1681 gggatatagc ttcaactgta gaagtatgac aaggggacgg gggtactgtc atctttttat
     1741 tcaaaacaaa gaaacagatt tgtctgttaa agggttattt ttattatggg aatattgata
     1801 gttctattgg ggggactctt atttttatat ttgaatatca aatgtgcatt taaaataaat
     1861 attacttttt attttttgca tgtttatttt tacgtaatta tttttagaaa aaaacatgaa
     1921 tatcaaaaaa gaatcaattt ttccgcaaaa aaaataattg acagatataa agctccggaa
     1981 ttaagacaaa agactaaaaa caatctaaaa tatcttaagc atataaaaaa agtatttaat
     2041 attttttatg taaaagatat ttttttctat cccgaatgta ttttggaaaa acaatcattt
     2101 gctcttgaat ttgtaatagt aaacagaatg cttaaaaaat ctcttcttaa cggatgatta
     2161 tgttataatt cttttttttt agggcataat taaaataacg gaagaaattg ccacgcgatt
     2221 tcaacactgt taaacgaggc agagaatata ctcaccattg tgattttgcc tcgttattga
     2281 tttccaaaag aaaggggata acatgagtaa ggatatagaa agtataatca aaacgacatt
     2341 agataatatt aataatttaa cagaaaacga aaagctcata agcagcaaaa tttgttatga
     2401 caataccaat tatttggcga tatccaagct taatatgaac tttattatag gaggaagcga
     2461 tatagataaa aaatttcgta cagttgattt aaagcccttc gccggagcag cttatgttaa
     2521 tctgcaatta aacccgcaaa ttttaattta tgaaacaccg gataataata tcaaaactat
     2581 taatattaac aatgaaacaa ctgcaggaga tattacatcc tccatcatga atattatgtc
     2641 atttattaaa gaaataaggg agaaaaagga ttgtgatatt taagagagca attgctatat
     2701 tgacagttat atttattttt acatgcgaat ttacatgtgt tgaagctatg cccggtgttt
     2761 cggcacaagc ttatattgtt atagatgctc aatcaggaag aattcttaaa tcccataatg
     2821 aaaataaaag actgcctatt gcaagcacaa ccaaaataat gacagcaatt ctcgcaattg
     2881 aaaccatcga tgatttaaac caaagaatag aagtgcctcc ggaatgctca ggcatagaag
     2941 gttcaagcat ctatttaaga cctaagcaaa tggtgtcggt aattgatttg ttatacggaa
     3001 caatgcttcg ctcgggaaac gatgcggcag aaacgttatc taaatttgcc ggaagaaaca
     3061 atccggaagg attcatatca atgatgaata aaaaagcaaa agagttaggc gctctaaata
     3121 caaattttga aaatgcaaac ggccttcatg acgataatca ttattcaaca gcatacgact
     3181 tggctcttat aagtgcctat gccatgaaaa atgatacatt cagaaaaatt gtgagtaccg
     3241 aaaagtacaa ggctgaaagt atgaacaata tgttctacaa taaaaataaa gttgtttacc
     3301 agtataaata cggtacggga attaaaatcg gctttacaaa ggcagcaggc cgatgtatag
     3361 ttgcatctgc agaaaaggac ggtactgaaa ttatagcagt gcttctcaat gacaataatt
     3421 ggtttaatga ctcttatcaa attttcgatt gggcttttga aaattacaaa tgctacagca
     3481 tagtcgaaaa agggcagttt gtaaaatata ctcccgaaag aaaccccgtc tttatcggag
     3541 agagctttag atgcgtattg acggataaag aaataaaaca gattcgtttt gaagcattaa
     3601 ttaaaccgga gcttatgtca aatgattccg aaactaaaac atgcggaacc tacaacatat
     3661 atttgggaaa aaccaagctt ggcacaggat atctgattag atgacaaggg gatgggggct
     3721 ttgtcatgtt tttgttcacg cccaccaggg ttgcttcctg aaaacgcata ttgaatattt
     3781 tacaaattca tacatttata atataatatc tagaaaacag ggaaaagagc ccatcccttg
     3841 ttatccggag gagatatgga agaaagactg caaaaatata ttgcacgatg cggagttgct
     3901 tcgagacgta aagctgagga aataatttta caaggtaatg taaaagttaa cggaataatt
     3961 ataaaagagc ttggaagcaa aattaatcct gaaagggata ttgtaaccgt aaataatcaa
     4021 aggatatatg aaaaacaaaa aaatatttat ataaaaatgt acaaacctga ggggtatatt
     4081 acaaccgtca aggaccagtt taatcgaaaa actgttcttg acttaatcaa tattaaagaa
     4141 aggatttatc ccgtaggaag acttgactat gacacttccg gattactgtt attaactgat
     4201 gatggagacc ttgccaacaa attaatgcat cccaaatatc gcatcttcaa aacctataaa
     4261 gcggaaattg aaggaggaat aagcgaagat tccataaata aattaagaaa cggcgtttta
     4321 attgacggat acaagactgc tcctgccaag gtaaaattgt taaagaaaac tcaaaagaat
     4381 tcgttcgtac aaataagcat tcatgagggt aaaaacaggc aggtaagaaa aatgtttgag
     4441 aaggtaggcc acagggtgat aaaattgaaa agactatcat tcggcaatat caatttagac
     4501 ggactgagag aaggacaatg gaaatattta accgatgatg aaatagaata tttaaagagg
     4561 taaagatgga caagatgtac ggtcaaatta cggaaatggt gcactttcta atcaagggaa
     4621 gctatgaagg aaaagaggat ttaagatttg ttgatttaac gtgcggaaac ggatatgaca
     4681 ctctattttt aagcaattta gcagggaaaa aaggttttgt aacagccttt gacatacaag
     4741 aaacagcaat tgagagaaca aaaaaactac ttcaagaaga aagtaacttc aataattata
     4801 aagttattca taatggacat gagcatatag acaaatattt gacggaaaaa atagatgcgg
     4861 ccatatataa cttaggatat cttcctaatt ttaacaaaga tatttttaca aaaccggaaa
     4921 ctactatctg ctctataaat tcactttttc cttatttaaa aaactcagga aggatatata
     4981 ttacagctta tacaagtcac gacaaaggct gtgaaattaa ttcaatttac gactacttaa
     5041 ttaagttaga caagaaagaa tacaatgtaa ttcatataaa agttataaat aaaaataaca
     5101 ctcctcctga attatttatt atagaaaaaa attcataata ctaattaatg acaatacccc
     5161 cgtccccttg tcatatgata ctgcttgctc atttcttgca aaatattcat ttatatgtgc
     5221 gatttagtta aaaagcaacg ttttccttcc aattaaatgc agataatgaa ataaaacttg
     5281 cattatgcaa tattatttga tatatttctt gcaagaaaac ctcgaatctg ttataattaa
     5341 gataacgaaa ttatatggag gaattatatg gctgaggaaa aaattctttt acaaaatata
     5401 ccatggtgca aaggatgtgg tgtatgcgtt ggtttttgtc ccgtaaaagt actggaaatc
     5461 aaagacgata aatgtgtcat agctcacagg gataaatgta ttttttgtgg acaatgtgaa
     5521 ctaagatgtc cggacaatgc tatttggatt ggaggtaaaa aatgagtaat aaggtataca
     5581 aattagttca aggaaatgaa gcttgcgttg aaggtgcatt agctgccggt atgagatttt
     5641 ttgccggtta tcccataacg ccatcaaccg aaatagcgga aatatctgca gaaaagctgc
     5701 cagcgctggg aggcagattt attcagatgg aagatgagct tgcaagtatg gctgccataa
     5761 taggaggctc tctcgcaggg aaaaaatcta tgactgcaac cagcggtccc gggttttctt
     5821 taatgcagga aaacataggg tatgcagcta ttaccgaaat accttgtgtt gttgtaaatg
     5881 tgcaaagagg tggaccgagt acaggacttc cgacatctcc tgctcaaggt gatgttatgc
     5941 aggcaaagtg gggaacacac ggagaccatc ctgctatagc cttgacacct aattctgttc
     6001 aagaaacata tgaatatact gttaaagctt ttaatttagc tgaaaaatac agaactccgg
     6061 tcatactcct aatggatgaa acagtaggac atatgaggga aaaaatggct tttaaagatg
     6121 tttcggaaat cgaaataata gacagaaaaa aaccaacatg caagactgag gactatgttg
     6181 cttatagacc tgatgaagat atgataccac caatggcatc attcggagaa ggatacagat
     6241 accatgtatc gggacttact catgacgaaa ccggttttcc tacaaataat aatgaaatat
     6301 ccgaaaaatt agttaaacga ttagttgata aagttaataa aaatttagat gacttttcat
     6361 attatgaaga atataaatta gaagatgcag aaattgttat aatcgcatac ggcggagtat
     6421 caaggtcagc taagagtgct gtagacgagc taaggaaaca aaatattaaa gcaggtttat
     6481 tcagaccaat caccatttgg cctttcttag aaaaacaagt aaataaggtt tcattaaacg
     6541 caaaagaaat atatgttgca gaaatgaact tgggacaata ttttcttgaa gttgaaaggg
     6601 ctgcatgcaa aaattgtact gttaaaaata taggtaaagt aaatggtgaa ctaatcactc
     6661 ctgaagaaat tgtacaagca ataggaggta acaaataatg aactgtaacg aaataactaa
     6721 aaaatattac agagctaaca agctccctca tatatggtgt ccgggatgcg gtcatggcac
     6781 attaacaaat acaatagtaa gagcaataga taaattagga cttaataaag atgaggtagt
     6841 aatagtatca ggtatcggat gttcatcaag agctcccggt tatttggatt ttaatactct
     6901 tcacacaact catggaaggg cattggcttt tgcaacgggt attaaatttg caaatccaaa
     6961 tcttcacgtt attgtaataa tgggagacgg agacagttct gcaattggag gcaatcacct
     7021 aatccatgcc gcaagaagaa atatagatat aacagcagta gtattcaaca ataatattta
     7081 cggaatgaca ggcggacaat tttcacctac tactccttcc ggtgatatgg caacaactac
     7141 accacacggc aatattgata aaccttttga cctttgcagc ttggctcaag gagcaggagc
     7201 tacttatgtc gccagaggta cagcttacca tgctgttctt atgcagaaat taatagaaaa
     7261 cggaatacag aacaaaggat tttcgtttat agaaggttta agcctgtgtc caacatatta
     7321 cggaagaaaa aacaaaaaag gcagtgcttt taaaatgcat acttttttaa aggaaaattg
     7381 tgttgataaa aaggtagttg ataaagatcc tgaaaaaggc aagaataaaa tattaatcgg
     7441 cgaattttac aacaatccgc agcctgaata tacagaaagt tatcaagctg ttattgataa
     7501 acttcaagga tagggggaga aaaatgaaaa aattagagat aagacttagc ggttccggag
     7561 gtcagggagt tatattagca gccattatat tagcgaatgc cggagtcgaa caaggattaa
     7621 atgctataca aactcagtcc tacggacctg aagcaagggg cggtgccagt aaggcagaag
     7681 ttatattgag caatggtgaa ataaattatc ctaaaatatc aacggcaaat atacttcttt
     7741 ctcttactca aaaagcatat gataaataca aatcatctct ggaaaaagat ggtatattaa
     7801 ttgttgatga aagtgttaaa ctaaggggag acgaaacatt taaagtatat agtcttccca
     7861 tattgagaac tgcactagag aaatttgaaa ctcatatggt agccaatata atttcattag
     7921 gtgcaattta tgaattgatg ggagaaaaca taattgataa ggatataatg atacgctcca
     7981 taactgaaag agttccggct catacagttc aaaaaaacac ggatgcattt gaagaaggta
     8041 ttaaattagc aagagggtaa catgaatgat ttaataagca ttatcaaaaa aaactttcat
     8101 aaattcagca gaggacaaaa gctaatagct caatttatta ttgaccacta tgacaaagct
     8161 gcatttatga ctgctgcaaa aatagcagaa acagtagatg taagcgaatc aaccgttgta
     8221 agatttgcat cggccttggg ttattccgga tatcctgaaa tgctaaaagc tttacaggtt
     8281 ttaattaaaa ataaacttac cacggtccag agaataagtc ttaatgatga tgtaaatgac
     8341 aagttaaagc ttcataaaag aaatttaaag aatgagatga acaacttgcg ttatttgtat
     8401 gaccatttcg atatggaagc attagataaa gcaaccgagc taatcttaga ggctgacaga
     8461 gtatttgtac tagggcttag aacatcttca actttgtcaa attatctagg cttctatttg
     8521 gatgtaatct tgaataacgc taaagtactc aacaacagcg gtgttaattc cttatatgaa
     8581 gaaattataa gaattaaaga agatgattta ttgattatta ttagttaccc aagatattcc
     8641 agaacaactc ttgatgctgc aagatttgtt aaggagcgaa atacaaaaat agttgcaatt
     8701 acagataccg aagaatcacc tgcctatgaa ttggcagatg tatcactctt atcaaaaagc
     8761 aacattgtat catttgttga ctccttagta gtacctatgg caatgataaa ccaattgata
     8821 atagatataa gcttgaggga aaaagaagaa atagttgaat actttaatac attagaaaga
     8881 ctctgggacc ataacagtat atatcagaac ttctaatata tgtgaggtaa tgggacacac
     8941 ctctgttttg ggaaagtctc tggatcagag gaatgtcctt aaattaaaag agcgacgctc
     9001 taaagtaaga ataatcatta aggatataaa gaaaggggag acgcttataa caagagcatg
     9061 tttattgagc gttaaggaaa taccctatac tttgtatcct tttgttaact gtgccaaaaa
     9121 attttcttgt tatatttaat aatatgtact ataatgttaa ggtgttgaat acataatagg
     9181 gacattatgt gcaacagtaa atactaagtt tggttttacc atacttatat attatgaaag
     9241 gaaaacaata tgataatcgc aatagacgga ccgtcaggag ccggaaaaag cactgttgca
     9301 agactattaa gcaaagaact tggatatgag tacatcgata caggggctat gtaccgagct
     9361 cttgcatata aagcatataa acaaaatatt gacataaatg aagctaatat tgcagaattg
     9421 cttgaaacaa ccaatattac ctactatgac aataaagtat ttttagacgg tgaaaatgtt
     9481 gaaaacttaa taagaaatga agaaatatcc atagcggctt caaaaatatc atctttaaaa
     9541 attgtcagag aaaaaatggt tgaaatccaa agaaagattg caaaaaataa aaatgtggta
     9601 ttggaaggtc gtgatattgg tacaattgtt tttccggatg cagagcacaa atttttcata
     9661 actgcatcat tggaagagag agcaaaaagg cgttacgagc aattgaaatt aaacaatatt
     9721 aaagctgatt acacaaatgt tatcaatgat atgattaagc gtgacgaaaa tgattcaaca
     9781 agaaagtttt ctccccttaa acctgctgaa gatgcaatat taatagacac aacgaatatg
     9841 gacttgaatg aagttacaaa gactattgca caatatatcg gaggaagcaa tgttttataa
     9901 aatagcagtg attgtattaa aggttcttgt atttataatt tttgacttag aagtatataa
     9961 cagagagaac cttgacaata tagacggtgg cttaataatt tgcggaaatc acagatccat
    10021 gattgaccct gtaatattag cagtatctac taagcggcaa attcacttca tgggcaaaaa
    10081 agaattgttt gaaaacaaat tcctatcatt tttattcaga agtcttggtg cctttcccgt
    10141 cgacaggaaa ggagtatcct tatcggcaat aaaaaactcc cttaatgttt taaataaggg
    10201 cggtatatta ggaatatatc cggaaggaac aagaattaaa accggatttg acgaaaaaaa
    10261 tgcaaaatcg ggaattgcct taatagctaa taaagcaaaa gtcggaatta tgcccgttta
    10321 tttggatgga ccatataaat tcagaggaaa actcagactg tatataggag aagaaaaaga
    10381 ctacttcaaa aattacagcg gcaggctgaa ttctgaagat tatacggaaa taggaataga
    10441 aattttaaag gatatttaca gattaaaaaa tgaaggtatg aaatgaaaat aattgtttca
    10501 gaatacaacg gtttttgcgg aggagtcaag gctgccgtac tgaaagcgga taagattttg
    10561 actgacgaaa aaaaactata ctgttacgga gaaataattc acaacaaaga tgttgttgaa
    10621 aaattaaaaa acaaaggaat ggttattgtt gacgatattc ctgaaaacaa ggatgcaaaa
    10681 tttcttatca gaagtcacgg tgtaggaaag aatatatatg acagattgga agaaaaaaac
    10741 atcgaagtta ttgatgcaac ttgtgtaaaa gtgaaaaaaa tacataaaat agttgaagaa
    10801 ttcaaaaata aagattacaa tataatcatt gtcggcaatg agaatcatcc tgaggtacag
    10861 ggaatatcgg gctggtgcaa ctatgaagcc gtaattatta aaagccctga agaactaata
    10921 aatattattg agccgacaaa aaaatactgt ttagtctgcc agacaacatt taatttaaat
    10981 acttttgaag aaatattaaa aactattgaa ataaacaatt attctaatat aattgtacat
    11041 aatacaatat gcgatgcgac aaggaaaaga caagaagcat gcattgctct tgcttccaaa
    11101 tgtgatataa tgcttattgt aggcggtaaa aacagttcaa acaccaaaaa gttatatgaa
    11161 ctgagcaagg aattgtgcga aaacacattc ttaatacaaa attataagga aataccttat
    11221 aactatattg ataaaaattc tattgtggga gtatccgcag gtgcttcagc tcctgactgg
    11281 ataattgagg aggttattaa tatgttggaa aatttgaata acaacatgaa tgaaatgaac
    11341 gagcaggcag aacatgcaga tttagctgaa cagtccgagc cggcagaact gtctgataaa
    11401 aaggaacaga cagaattagt agagcaatcc gaggaaacag aaaagacaga aactgttgag
    11461 cagactgata aagcagaaaa agtaaacagt atgcatgaat tgcttgaaaa ttatggcgga
    11521 gtagttaatc tcggaatcgg cgaaaaggtt aaaggaacag taatatatgt aagtcctgat
    11581 gccgtttctg ttaacatcaa ttataaatct gacggaatta taacaagaga agatttttcc
    11641 ttggaagaag tttccgattt aactgaagta gtaaaagaag gagacgaaat tgatgcaatt
    11701 gtagtaaaaa tttctgacca ggacggaaat gttgtactaa ccagaaaacc attggaagaa
    11761 cagaaaatct gggacgattt ggagcagatg agaatccgag gagaaattgt ggaaattcct
    11821 ataattgaag catctcagca cggaatttac ggaaaactaa aaggcatcaa aggttttata
    11881 ccaagaaatc aaatttcaat aaaaagaaat gttgaccctt cggaatacgt aggcaaaaat
    11941 ctgaaggtta agatattgga aacaaagaat aaaaaaggaa gaagacagtt ggttttaact
    12001 tcaagagctg ttgaaaaatt tgaaaaggaa attaaagaca atgaagtatg ggaaaccatt
    12061 caagaaggag aaatttacga aggcacggtg aaaaaccttc aggattacgg tgcatttgta
    12121 gaaatcaacg gtatcgatgg acttcttcac atcaatgaaa ttgcatggac cagaattaaa
    12181 cacccgtctg aagtgttaaa accgggcgac aaaataaatg taaaggtaaa aaatgtagat
    12241 aaagaaaata aaagactttc attaagttat aaagcaaccg taaaaagccc ttgggcaaaa
    12301 ttcttggata aatatcaaaa gggcgatatt gtaacaggag tagtaactcg tattgttgac
    12361 tttggagcat ttgttaaagt agatgatatt gaatgtctct tacacataaa agatttggat
    12421 tgggcaagaa cagaaaaggt aactgatatt ctccaggaag ggcaggaggt aactgcaaaa
    12481 atacttagca ttaccagaaa agacagaaag gttggccttg gattaaagca attgactgag
    12541 catccgtttg atctttatgc taagacacta aataaggacg atataataca ggttgaagta
    12601 acacgtatac tcttggacgg tatacatgtt aacgctaatg gaaatattga ctgcttcata
    12661 ccgattgcaa aggtgtcaac agaaaaatta agaacgccgg cacaagttgt aaaggttgga
    12721 gaaatagtag atgctaaagt gcaaagtatt gatatgaaag gtaaaaacct taacctgaca
    12781 ttaatattag atgacaaaag tgaagaaact gtagaaaata aatcttatca atcagaagat
    12841 acaaacttta ctataggaga atctatagga gatgcactgg atgatttatt aaaataattt
    12901 ctaatgttag gtcgcaagat ctaacatttt ttttgaaaaa taggaggcaa aatgcttaaa
    12961 gaatttttgt ttgatatatg ctccaaccaa tttgtttcag gttttgagca tataaacggt
    13021 gatattttga taaaatattt taagccttat actgattctt ttgaaaagga caggcttgga
    13081 agttatatat ttaaaagctc cggcagtaag gacaccaaaa taatgcttgc tgcacatatt
    13141 gacgaaatag ggcttatgat tacaggcata ttagatggag gatttttgag tttcattacg
    13201 gtgggaggat ttaatcctgc atcattgatt gcccaggatg taacaatatt cggaaaagaa
    13261 aaaatatttg gagttatagg aattaaaccg cctcatttaa caagtgagga agaaagaaaa
    13321 aaaccgctta aaacaaggga attatatata gatacggggt acacaaaaga aaaacttgaa
    13381 aaattaataa caattggaga tattgcaaca ataaacagag aacctttatc attacaggga
    13441 agcgttataa gcgccaaagc atttgatgac agggcatgtg ttgcagttat gctcgaaaca
    13501 gcaaaagaat taaaaaaaat atcacataaa agcaatgtat attttactgc aacagctcag
    13561 gaagaaacag gtttaagagg tgccaaaaca agcagttata aaattgctcc cgatatcgga
    13621 atagtcttgg atgtaggatt tggcaaaact cccgatatgc cttccgattc ggctctctta
    13681 ggtaaaggcc caattgttgc atacggagga aggctaaata caaaacttac aaagaaattt
    13741 gttgaaattt gcaagaaata caactatcct atgcagtatg aagcaattcc tgttagaacc
    13801 ggtacagaca ctgatgcact acaaatatca agagacggaa ttccctgcat acttttatca
    13861 attcctctca gatacatgca tacatcagtg gaaacaatag atttaaagga tttagaaatt
    13921 acaggaaaag ctatagcaag gtttataaat gaattggaca attccgattt ggaggaaata
    13981 ttatgttttt aaatgaatta actaaacttt ccggcgtatc aggctgtgaa tttgaagtaa
    14041 gaaattatat aaagagtaaa ctgtcggaaa tgggctgtca attctatgta gataagcttg
    14101 gaaatattat tgctcacaac aaaggcaaaa aaaataaaac cattatggtt tccgctcata
    14161 tggatgaagt aggattaata gttactaata ttgatagcga cggttttatt aagtttaaag
    14221 cagttggagg aattgaccaa agagttttaa actcaaaggt agtattaata ggagaaaata
    14281 atattccggg cgttataggc tctaaagcag tacatttgat gactgaaaaa gaaaaagggg
    14341 tctcccttcc aattgataaa ctatatatcg atattggtac aagcacaaaa gaggaaacag
    14401 aaaaattagt taatatagga gactacgcag cttttaaaag cgattatgtt gaattcggcg
    14461 acaatatgat taaagctaaa gccttggaca acagggtcgg atgcagcatt atattggaga
    14521 ttctaaaaga gaaattggat gtagattttt atggcgtatt taccgttatg gaggaagtcg
    14581 gattaagagg agcagaaaca gccgcatatc aattagagcc ggacttagcc atagtattgg
    14641 agggaaccgt atgcgcagat atgcctgaag tagaagaaca tgaaaactcc accttaatag
    14701 gcaagggagc agtattgtca ttaatggaca atgctacggt atatgatatc gaccttgtaa
    14761 gaagagttgc aaaaattgct gaagacaata atattgcata tcagtacaga cgctcaactt
    14821 caggggcaaa tgatgccggt gttatccata aaacaaaaaa aggcgcaaaa gtagtgtcca
    14881 tttcagttcc ttgcagatat atacattcat cagtaagcgt tgcaagcaaa aatgacttaa
    14941 tgagtgcttt aaagcttaca aaacaagtga ttattgaaaa tcagaaaggg gaataaaatg
    15001 tttaacagcg aattaatgaa aagattatcc gattgtttcg gtccttcagg aagagaaaaa
    15061 aggattcgcg aaatgattat ggatgaaata aaagattttg cagatgaaat aaccatagac
    15121 ccactcggta accttatagc aagaaaaaag ggtacaggca aaaaaatact gttttctgct
    15181 cacatggacc aaataggact gattataacc catgttgatg aaaaaggatt attgcgtttt
    15241 gcaaacgtgg gcggattaaa tgccaaagaa ttattgggac tccgcatggt ttttgataat
    15301 ggacttgaag gagtaatttg tcaagaagaa ttaaaagata atgaaaaact ttcaacaagc
    15361 aaattatttt tggatgttgc ttctaccgac aaagaattta ttgcaaagaa ttttcgagta
    15421 ggtgatatgt gtgtattcaa atcagaatat tacgaaacag aggactgtgt aatatgcaag
    15481 gctgcggatg acagaatagg ttgctatgtc ttaatcgaag cactaaaaaa tcatccggaa
    15541 acaaacaatg atgtatatta tgtgttttcg gtgcaggaag aagtgggctg cagaggagct
    15601 aaaacggcag gatacaacat taatccggat ttggcaatag cagtagatgt aactgcaacc
    15661 ggcgatacac ctgacggaat aaaaatggat gttaagttag gcaatggagc agcaataaag
    15721 ttaatggata aatcaatcat tacacatacg gaagtaaaag acttattaac agatattgca
    15781 gaaaaaaata gtattaaata tcaatatgaa attttggaat tcggcggaac ggatgcagga
    15841 ccaattcata catccagaga tggagtgccg agcggtgtaa tatccattcc caccagaaat
    15901 cttcatacct ccggagaaat attcaataaa ttcgacgtag aagaatgcat aaaattagta
    15961 ataggaacag ctgcaaaata accctcaatt attatgaaat aacaaaaata tgttaaattt
    16021 agggataata tcacaaatta taggaaataa t
//
LOCUS       DAOSOW010000004        15772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_3740_length_15772_cov_4.113253, whole genome shotgun sequence.
ACCESSION   DAOSOW010000004 DAOSOW010000000
VERSION     DAOSOW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 15772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15772
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3740_length_15772_cov_4.113253"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLD67_00340"
     CDS             complement(<1..731)
                     /locus_tag="PLD67_00340"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOT21006.1"
                     /translation="MLNFKKIELEDKEWIIPILKESVITGCHQNFGNLFAWRDINNTR
                     VANINNFLVANQLVNGKDVYLYPMGKGDVKPVIEEIIRSADKNLIFAGLSHDEMIELN
                     ELYPGKFIYDQVRNDFDYIYLLEKMVSLKGKKLHRKRNHISAFLRNNPVWSFETITEE
                     NKAECIEMNKKWCIESGCKDDESLMDENCATKYFFKYYDELELEGGLIRVEDEIVAYT
                     IGEVLNNDTYVLHIEKSFKDVQGAYP"
     gene            complement(837..1415)
                     /locus_tag="PLD67_00345"
     CDS             complement(837..1415)
                     /locus_tag="PLD67_00345"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOT21007.1"
                     /translation="MNLEFAEYSEIFIKYLETWGPAAAIILPVFEAFLPILPLVGFVI
                     INVAAFGFFFGYLYSWIGNCLGSFLLFLLLRKIGGKRINEKIKSSKFRYTLEKVKKKQ
                     LNVLFLLYCFPFAPSFLLSGTAALTNMRTEMFLAILLPSKFVMLLSLAFIGENVSSFF
                     ENPAKSIFYIIFIVLLNLLVKYLFEKYEQRKQ"
     gene            1662..2162
                     /locus_tag="PLD67_00350"
     CDS             1662..2162
                     /locus_tag="PLD67_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HOT21008.1"
                     /translation="MKYFIGAPIPDNYKNKIEMLRAEFRFFTTEPHITLVPPPALPDE
                     DDFIEEVVEVCKKVKPFDIRLNNLDQFGSRVLYVSVDSPGLIDLHNKIYEKLCLQHER
                     RGFTPHLTIVKQRPKRPVDIATIRKRAEIKLIPSPEYTLNSITVYHQPKEHSIYIPYM
                     EIPLGE"
     gene            complement(2196..6974)
                     /locus_tag="PLD67_00355"
     CDS             complement(2196..6974)
                     /locus_tag="PLD67_00355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOT21009.1"
                     /translation="MSQITFIDAEVVPETGKIIDFGAIRPNGEYIHSASLDIIRGFLF
                     ENDFLCGHNIIAHDLKYLNKYLGQIKCVGVIDTLHLSALLFPQRPYHSLLKDDKLLTD
                     ELNNPVNDSKKAMDLFFDEIGAFKLLPEEMKKILYLLLNHKKEFKDFFQYIDYKSEGT
                     NLEAETEIRNFFNGRLCDNADFKHLIANHPIELAYCLSLINADDEYSITPPWVLMTYP
                     YVDSIMNQLRGTKCLFGCEYCNEMMDAVKGLKRFFGYNAYRSFDGVPLQEQAVKSAVN
                     GDSLLAVFPTGGGKSITFQVPALMAGRNTKSLSVVISPLQSLMKDQVDNLEKINITDA
                     VTINGLLDPLERADAIKRVENGDVHILYIAPESLRSKTIEKLLLGRKIARFIIDEAHC
                     FSAWGHDFRVDYMYIADFIKQLCEKKNLDYIIPVSCFTATAKRNVIEDIKKYFREKLN
                     LELKLYTANSGRENLTYKVIPNDRSEKYETLRNLIEYNNSPTIIYVSTTKKAEELAER
                     LTKDGYKAMAYHGRMDKKIKSQNQNEFIRGNVDIMVATSAFGMGVDKKDVGMVVHFDI
                     SDSLENYVQEAGRAGRDQNIKANCYVLFNNEDLDRHFMQLNRTKISIQEIQQIWMAIK
                     NITKMRSKISSSALEIAKKAGWDEGINDLETRVRTAILALENAGYIKRGQNTPRVYAN
                     SILVRNMKDASEKIQASKLFNDNEKQQAIRIMSKLISARSRKRASSEDAESRVDYISD
                     HLGIEKEQVIHIIQLLRQAGILSDNKDLTVFMEESSLGNKSMEKLYEFVELERFLLSH
                     ISNTTVNIKDLNELALDAGVKSSNPDKIKTVFNFWSIKGLVKRYAKGTYYLKIILLKD
                     KDEALKELEERWSIAEFILIYLQKINVNNENALEFSVLEIMGEYNFSMQLFNKKATQS
                     QIEDSLFFLSKIGILKIEGGFLVIYNAISIERLIKDNRIRYKAEDYKTLKDFYKHKME
                     MIHIVGEYAKKMMEDYNSALKFVDDYFRLEYSSFLKKYFKDSKEDELQINMTPDRFRQ
                     LFGQLSPAQLRIINDKDSKYILVAAGPGSGKTRILVHKLAAILQLEDVKNEQLLMITF
                     SRAAATEFKKRLSDLMGTKANYVEIKTFHSYCFDLLGLVGNIEKSDDIINNAINAIIN
                     NQVEQSRITKTVLVIDEAQDMDANEYKLVKTLMERNEDMRVIAVGDDDQNIFSFRGSD
                     SKYLSDILNFPNSKMYELVDNYRSTRSIVDFCNYFVRTMKKRLKSMSIAAVSKEKGSV
                     TITEYKSSNLIEPVTNKLIKNGIYGSTCILTMTNEEALQIYALLERNGIKAGMVQRGG
                     IFNLKNLLEIRYFIEEMKLMDGTPLINDEDWENAKKRTFIKYNESDNLPLLRQIISNF
                     EESSGKYKYKSDFSMFIEESREEDFYTGTDKITVSTIHKSKGKEFDHVIMVLRDLNLN
                     REETKRLIYVGMTRAKKSLSIHFNGKFIERKIGFIDMILRNIKYEFDNEEYPPAEIIS
                     MQLKYNDVYLSYFYKTIKLVQKLNSGDILHFDENGCKDENGVYILLFSSKFKNEIQTY
                     IEKGYKPFSAKVNHILFWKEENKEKETLIVLPQLTFIKSN"
     gene            complement(7153..8499)
                     /gene="glmM"
                     /locus_tag="PLD67_00360"
     CDS             complement(7153..8499)
                     /gene="glmM"
                     /locus_tag="PLD67_00360"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOT21010.1"
                     /translation="MGKLFGTDGVRGIANKDLSPELAYKLGRVGGYFLTNGIKRPKMV
                     VGLDTRISGDMLEGALSSGLNSAGIDVLYLGILPTPAVACMIKILDADGGVMITASHN
                     PIEYNGIKFFDKDGLKLTDETENLIEEYINNNLDIDYIPISGDLGRKIRVEAPLRKYL
                     DYLKSTINVELNGLKIAVDCGNGAVYKAAPQLLHELGAQVYVIHNEPNGININVDCGS
                     TNPEEIQKLVLETGADVGLSFDGDADRLIAADENGNIVDGDHVLAICGTCLKEKGKLR
                     NNTIVGTVMTNLGLDICLKDKGINIIKTSVGDRYVLEEMLKSGYSLGGEQSGHIIFLD
                     FNTTGDGLLTAIQLLSVVKQKNAKLSELSAMMKSMPQVLVNAHVSASKKKDYLTDDVI
                     VNKIKEIEEHFSGKGRVLIRPSGTEPLVRVMIEGENQEEINFHAAELAKLIEERLQ"
     gene            8721..10637
                     /locus_tag="PLD67_00365"
     CDS             8721..10637
                     /locus_tag="PLD67_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019108270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOT21011.1"
                     /translation="MIVLSCNNVSKAYMVENIIENISFTVNDNEKVGLIGLNGAGKTT
                     LFNILTENLEPDSGEIYVAKGKKLGYLKQNTIIESNMSIMDEMLLIFNDVIKMEEKLH
                     ELSDKISSISGEKGSAELEALMNTYSELSEQFVEADGYSFRSIIKGVLKGLGFSEDEF
                     DKPINILSGGQKSRVMLAQLLLKKADILLLDEPTNHLDIEAISWLEKYIKNFKGAVII
                     ISHDRYFLDNTVNRIFLMENKYLKSFNGNYTEFVKKRKIELEQAMKQYDEYEREIERQ
                     EEMIRRLHAYGSKRNIRQAFSRQKALDKIKKMDKPSVGSQKFKLKFTPRINSGRDVLS
                     VNNVAVSFDDQQVFKNVNLKIYKGEKVGIIGPNGIGKSTLLKIIAKIIKDYSGEIEYG
                     HYVNIGYYDQEQTNLNNENTVIDEIWDEVPNLNYYTIRKYLAQFLFYGDDIYKLIEDL
                     SGGEKGRLSLLKLMTSNANFLLLDEPTNHLDIDSKEVLEDSLINYEGTILVVSHDRYF
                     LNKVVNKIYDLSEDGAFEYLGNYDYYLEKKAELDETDDIDTITKTKTQINAEKKKERE
                     IIRDRQQREKTIKELEEQIDMHENEIHELEIKLCEPSTYNDNFLIINLNEKLNIRKKE
                     LQELYLLWEELMDN"
     gene            complement(10747..11778)
                     /gene="tsaD"
                     /locus_tag="PLD67_00370"
     CDS             complement(10747..11778)
                     /gene="tsaD"
                     /locus_tag="PLD67_00370"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOT21012.1"
                     /translation="MKENINEKTLILSIETSCDETAASVVENGRNVLSNIISSQIETH
                     KEFGGVVPEVASRKHIENINDVVSKAMSEANAEPKDIDAIAVTYGPGLVGALLVGINF
                     AKGLSYAWEKPLIGVNHIEGHINANYIENKELQPPFLCLVVSGGHTHLIHVKDYGVYK
                     ILGMTRDDAAGEAFDKVARAIGLGYPGGPLIDMAAKKGKRDAIKFPRAIIDDETLDFS
                     FSGLKSAVLNYLHNSKQKNETVNTEDIAASFQEAVVDVLVMKTMKAAKQENISTIALA
                     GGVASNSRLRERFKEECLMHNYIFMRPSPILCTDNAAMIGCAGYYKYLNKRFADSSLN
                     AIPNLELAN"
     gene            complement(12189..13490)
                     /locus_tag="PLD67_00375"
     CDS             complement(12189..13490)
                     /locus_tag="PLD67_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT21013.1"
                     /translation="MNNENTSERLNNSKVSTWLADNVVSLIFVAFTIFGFLVSDGVTM
                     NFFLTELSSRFFRNAFLVLSLIIPVVAGLGLNFGIVVGAMAGQIALAIVLYFGWGGVN
                     GLLLTMLIALPIATLFGYLTGLLYNKTRGQEMIASLMVGYFANGIYQFLFLFVVGVII
                     KVPNTHPMIKPDGVGVRMTVDMGAYDKGGLKYALDKIWEIPFMWAILAVFACILLLQI
                     WKIVRNKKNPTLYRYNMTMTAINIILSLVMIAISVHAITTNSSLMIVRKVPVITGLLI
                     VALCIFNELILKTKLGQDFRSVGQSQHIAEVSGINVDKTRIIATIMSTVFAAWGQIMY
                     LQNMGTLNTYGAHMQIGLFSVAALLVGGASTQKAGVKHAIFGTLLFNSMFIMSPEIGL
                     SLFGNALLGEYFRTFMVYGVIGLALGLHVWRTNKKGKITLN"
     gene            complement(13490..14590)
                     /locus_tag="PLD67_00380"
     CDS             complement(13490..14590)
                     /locus_tag="PLD67_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT21014.1"
                     /translation="MVEVKKKKATFNDIVNKIGFPTIFIGGFWILLLIAGGALGISII
                     TLLSDTIKRAGMNGILVLAMVPSIQSGTGPNFALPVGIVCGLFAIVCSIEFGFTGWAW
                     LFVAIAFAIPLATVVGYLYGRLLNAVKGSEMTIATYTGFSIVAAMCLAWLMIPFQSPN
                     MGWFIGKGLRETIQLDAIGGAHILSNFLRFSLFGENGLITINGETGIIIPTGMLLMFF
                     ICCTIVWLYFRSKSGIAISAGGINPKFAQSSGINIDRCRIKANVLSTILGAIGIIVYS
                     QGYGYAQLYTAPLMMAFAAVAAVLIGGASASRAKVSNVIIGVLIYQGLFTTALPVANQ
                     LFEGTDLSEILRMVIQNGIILYALTKVKGGDN"
     gene            complement(14590..>15772)
                     /locus_tag="PLD67_00385"
     CDS             complement(14590..>15772)
                     /locus_tag="PLD67_00385"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOT21015.1"
                     /translation="RKALDVLDMDIEEYVQVAGLPVGHMQFIEIAREIDKTGLKILVF
                     DEPTAVLAETEAANLLKAMKKLAQKGIGILFISHRLDEIIEVADVVTILRDGEHVVTK
                     NTEDTNKYEIAQLMVGRSITIEREESTNRNIVDETILDIKNLHVAMPGERVKGVDLKV
                     KKGEIVGIGGLAGQGKLGIANGIMGIFPAEGEVEFKGKKSALNNPKSAIKSGIAFVSE
                     DRKGVGLLLDESIESNIAFSAMQINDEFLVNYGPIKLKDSAKIREHANKMIKDLDIRC
                     TSSLQRVGSLSGGNQQKVCVARALTMKPELLLVSEPTRGIDIGAKKLVLDLLKQLNNE
                     GMTIIMTSSELAELRSICDRILIICEGKVEGVLPPDAPDAEFGLMMSGSRNGKSEGSE
                     A"
BASE COUNT         4889 a         3058 c         2445 g         5380 t
ORIGIN      
        1 ggatatgccc cttgaacatc tttaaacgat ttttcgatat gtaatacata tgtatcatta
       61 tttagaactt cacctatggt ataagccaca atttcatctt ctaccctgat taaaccgcct
      121 tcaagctcga gctcgtcata atatttaaag aaatatttag tggcacagtt ttcatccata
      181 agactttcat cgtctttaca tcctgattca atgcaccatt ttttgttcat ttcgatgcat
      241 tcagctttat tttcctcagt tattgtttca aatgaccaaa caggattatt tcttagaaag
      301 gcactgatat ggtttctttt tctgtgaagc tttttccctt ttaatgaaac cattttttcc
      361 aacagataaa tatagtcaaa gtcatttctt acttgatcat aaataaattt ccccggatac
      421 aattcattca gttcaatcat ttcatcatga gataatcctg caaagattag atttttatct
      481 gcagacctta ttatttcttc aattacgggt ttaacatctc cttttcccat tggataaaga
      541 tatacatctt ttccattaac taattgattg gctaccaaaa aattattgat attagcaact
      601 ctagtgttat ttatgtccct ccatgcaaac aaatttccga aattctgatg acatcctgta
      661 ataaccgatt ccttcaatat gggaataatc cattctttat cttccagttc aatcttttta
      721 aagttcagca ttaataattt tctccttgtc acgttgtcca atcgtatagt attttttcct
      781 cattagaaat atattacatt acacattata cactatatca taaaaaaaat caccaattac
      841 tgttttcgtt gttcgtattt ttcgaacaaa tacttcacta atagatttaa cagcacaata
      901 aaaataatat agaatattga ctttgcagga ttttcaaaaa atgagcttac attttcaccg
      961 ataaaagcca atgaaagaag catcacaaat tttgacggca gtaatatagc aaggaacatt
     1021 tccgtcctca tattagtcaa agctgcagtg ccgctcagta aaaatgacgg agcaaaagga
     1081 aagcagtata ataaaaacag cacgtttaat tgttttttct ttaccttttc aagggtgtag
     1141 cgaaatttgc tactttttat tttttcattt attctttttc ctccgatttt ccttaacaat
     1201 aagaataaaa gaaacgatcc taagcaatta ccaatccaag aatataagta tccgaagaaa
     1261 aatccaaaag cagctacatt tattattaca aatcctacca atggcagtat gggtaaaaat
     1321 gcttcaaaca caggaagaat tatggctgca gcaggtcccc acgtttctaa atattttatg
     1381 aaaatttcag aatattccgc aaattccaaa ttcattatca aacctccacc ggtcagacta
     1441 tctatattta ttatacccat cattagattt acatacaaaa caaacaaaac atttatataa
     1501 attaagtgaa acactattaa tattaacata aatgcttgat aattttaaat aaaaaatata
     1561 aaattatctc atgagattct tcacagtgtt cagaatgaca atatgtgtat tcatggcaag
     1621 acagggtaaa taattataaa aaagatatag aaaggattta tatgaaatat ttcataggag
     1681 cccccatacc tgataactat aaaaataaaa ttgaaatgct aagagctgaa tttagatttt
     1741 tcacaaccga acctcacatt actttggttc ctcctcctgc acttcctgat gaggatgact
     1801 ttattgagga agtagttgaa gtatgcaaaa aagttaagcc ttttgatata aggctgaata
     1861 atcttgacca gtttggcagc agagttttat atgtaagtgt tgattctccc ggtttaatcg
     1921 atttacataa taaaatatat gaaaaattat gcttgcagca tgagagaagg ggatttaccc
     1981 cacatttaac aattgtaaag caaagaccca aaagacctgt agatatagcc acaataagaa
     2041 aaagggcaga aataaaactt attccttccc cagaatatac tttgaattca attaccgtct
     2101 accatcaacc aaaggagcat tccatttata ttccctacat ggaaattcct ttgggagaat
     2161 aacacagact gttcttctct gaaattcctc tgtaatcagt ttgatttgat aaatgttagt
     2221 tgagggagaa ctatcaatgt ttccttttcc ttgttctctt ccttccagaa taatatgtga
     2281 ttaactttag cagaaaaagg tttgtagcct ttctctatat aagtttgaat ttcgttttta
     2341 aactttgatg aaaaaagtaa tatatataca ccattttcgt ccttacagcc gttttcgtca
     2401 aaatgtagta tatcaccact attcagcttt tgaactaatt ttatggtttt gtaaaaatac
     2461 gacagataca catcgttata ctttaattgc atggaaataa tttctgcagg cggatattct
     2521 tcattatcga attcatattt aatgtttctc aaaatcatat caatgaatcc gatttttctt
     2581 tcaataaatt taccgttgaa atggatggat aaggactttt tagctcttgt cattccaaca
     2641 tatatcagcc tttttgtttc ttcccggttt aaattcaagt cccttaatac cataatgaca
     2701 tggtcaaact cttttccttt ggatttatga atagtggaga ccgttatttt atcagtgccg
     2761 gtataaaaat cttcttctct agattcttca ataaacatgg aaaaatcaga cttatattta
     2821 tattttccgg aactttcttc gaaattggat attatttgtc ttaataaagg cagattatca
     2881 ctttcattat actttataaa tgttcttttt ttcgcatttt cccagtcttc atcattaatt
     2941 aatggagttc catccattaa tttcatttcc tctatgaagt atcttatctc aagaaggttt
     3001 ttcagattaa atattcctcc tctttgaacc attccagcct ttattccgtt cctttcaagc
     3061 agtgcatata tttgaagagc ttcttcgtta gtcatagtaa gaatgcaggt agaaccgtag
     3121 ataccatttt tgattaattt atttgttaca ggctcgatta aattactgct tttgtactct
     3181 gttatggtaa cactaccttt ttcttttgat actgctgcta tagacatgct ttttagacgt
     3241 tttttcattg ttcgtacaaa ataattgcaa aaatcaacaa tacttcttgt acttctgtaa
     3301 ttatccacca attcatacat tttagagttt ggaaaattta aaatatcgct caaatatttt
     3361 gagtctgagc ccctgaatga aaaaatattt tggtcatcat ctccgacagc tattaccctc
     3421 atatcctcat ttctttccat aagggttttc acaagtttgt attcattagc gtccatatcc
     3481 tgtgcttcat caattaccaa taccgtcttt gtgattcttg actgctccac ttgattgttt
     3541 attatagcat ttattgcgtt gttgattata tcgtctgatt tttctatatt tcctaccaac
     3601 cctaacaaat caaagcaata ggaatgaaat gtttttattt ctacgtagtt tgcctttgtt
     3661 cccatgagat cagataatct ttttttaaat tctgtagctg cagcccttga aaaagtaatc
     3721 attaaaagct gctcattttt cacatcttca agctgtaata tagctgctaa tttgtgtact
     3781 aaaatccttg ttttgccgct tcccggacct gctgccacta aaatatattt agaatcttta
     3841 tcatttatta ttcttaattg agcaggtgaa agctgaccga aaagctgtct gaatctgtcc
     3901 ggagtcatgt ttatttggag ctcatcttct tttgaatctt taaaatattt tttcaaaaat
     3961 gagctgtatt ccaatcgaaa ataatcatcc acgaatttaa gtgctgaatt gtagtcttcc
     4021 atcatctttt ttgcatattc gcccactata tgtatcattt ccatcttatg tttgtaaaaa
     4081 tctttaagag tcttatagtc ctcagccttg tatctaatcc tgttatcctt gattaatctc
     4141 tctatactga ttgcattgta tataacaaga aagccccctt cgatttttag aattcctatt
     4201 tttgataaga agaaaagtga atcctctatc tgcgattggg tggctttttt attaaaaagc
     4261 tgcatgctaa aattatattc tcccataatt tcaaggacag aaaattccaa ggcattttca
     4321 ttatttacat ttattttttg aaggtatatt aggataaatt ctgcaataga ccacctttct
     4381 tctaactcct tcaaggcctc atctttatct tttaaaagaa tgatcttaag ataataagta
     4441 ccttttgcat atcgctttac aagacccttt atcgaccaaa aattaaagac tgtttttatc
     4501 ttatcaggat tagaactttt tacaccggcg tccaaagcaa gctcatttaa atcttttata
     4561 tttacggttg tgtttgaaat atgcgaaagc aaaaaacgct ccaattcaac aaattcgtaa
     4621 agtttttcca tggatttgtt tcctaaactg ctctcttcca taaaaacagt caaatcttta
     4681 ttatcggata atatgcccgc ttgtcttaat agttgaataa tatgtataac ctgttccttc
     4741 tcaattccca aatgatctga aatgtaatca accctggatt ctgcatcttc actgcttgcc
     4801 ctttttcggc ttcttgctga tataagtttg ctcattattc ttatagcctg ctgtttttca
     4861 ttatcattaa acaacttgga agcttgaatt ttttctgatg catccttcat gtttcttacc
     4921 aatatactgt ttgcatatac tctgggtgtg ttttgccctc gcttaatata acctgcgttt
     4981 tccaaagcta aaattgccgt tctgactctt gtttcaaggt cattaatacc ttcatcccag
     5041 ccggctttct tggcaatttc cagtgctgaa cttgatatct ttgatctcat cttggttata
     5101 ttctttattg ccatccagat ttgctgtatt tcttgtatgc tgatttttgt tcgattcagt
     5161 tgcatgaaat gcctgtctaa atcctcatta ttaaataaaa cataacaatt ggcttttatg
     5221 ttttggtctc tgccggctcg tccagcttcc tgaacgtaat tttccagtga atcggaaata
     5281 tcgaaatgta caaccattcc cacatctttt ttatctacac ccataccaaa tgctgaagtt
     5341 gcaaccataa tgtcaacatt accacgaatg aattcattct ggttttgact ttttattttt
     5401 ttatccattc gcccatggta agccatggct ttatatccgt ctttggtaag tctttctgca
     5461 agttcttcgg cttttttagt ggttgaaaca tagatgatgg taggagaatt gttgtattca
     5521 ataaggtttc tcagtgtttc gtatttctcg gatctatcat taggtattac tttgtaggtg
     5581 aggttttccc tccctgaatt cgcagtataa agctttaatt ccaaattgag tttttctctg
     5641 aaatatttct taatatcttc tataacattt cgttttgctg tagctgtaaa gcatgaaacc
     5701 ggtattatgt agtcaaggtt tttcttttca cacagctgtt taataaaatc agcaatatac
     5761 atataatcaa ctctaaaatc atgcccccag gcagaaaaac aatgggcttc atcaattatg
     5821 aatcttgcaa tcttacggcc aagtaaaagt ttttcaatgg ttttcgacct aagagattca
     5881 ggcgcaatat ataatatatg aacatcgccg ttttcaactc ttttaatggc atctgctctt
     5941 tctaatgggt ctaaaagccc gttgatagtt acagcatcag taatattgat tttctctaaa
     6001 ttatcaacct ggtctttcat aagagattgt agtggtgaaa ttacaacact aagagatttt
     6061 gtattccttc cagccataag tgcaggaact tggaatgtaa tagatttgcc tccgcccgtg
     6121 gggaaaactg ctaaaagaga atctccgttt actgcgcttt ttactgcttg ttcctgcaag
     6181 ggaacaccat cgaaacttct gtatgcgtta taaccaaaaa acctttttaa gccttttact
     6241 gcatccatca tttcattgca atactcacaa ccaaaaagac actttgtgcc ccgaagttga
     6301 ttcatgatac tatctacata tggatatgtc ataagtaccc aaggaggcgt tatggaatac
     6361 tcatcatctg catttataag agaaaggcaa taagcaagct caatcggatg attagctata
     6421 aggtgtttaa aatctgcatt atcgcataat ctgccattga aaaaattcct tatctctgtt
     6481 tcagcttcta aatttgtacc ttcacttttg taatcgatat attgaaagaa atctttaaac
     6541 tcctttttat gattcaataa taaatataat atctttttca tttcctctgg cagtaattta
     6601 aaagctccta tttcatcgaa gaataaatcc attgccttct tagaatcatt tacaggattg
     6661 tttagctcgt ctgttaacag cttatcgtcc tttaacaatg aatgataggg tctttgagga
     6721 aataaaagag cagataaatg caaggtatct ataacaccga cacatttaat ctgccctaaa
     6781 tatttattta aatattttaa atcatgtgca ataatattat gtccgcataa aaagtcgttt
     6841 tcgaaaagaa atcctctgat gatatcaaga gaagcgctat ggatgtattc tccgtttggt
     6901 cttatagcac caaagtcaat gatttttcct gtttccggca ctacttccgc atcaataaat
     6961 gttatttgac tcataatacc cgtatcctta gtcgaattat cttattttag ctaaattata
     7021 tagcatattg gaaaaaattt caatgaaaac aagaaaattc atctggtaac atagagacgg
     7081 accttatata gattgagaaa tactctgata gcaaaaagaa ccgtccaagg aaacgtctga
     7141 aacagaaact ttttattgaa gtcgctcttc tatcagtttt gctaattcag cggcatggaa
     7201 gtttatttct tcctggtttt ctccttcaat cataaccctt acaaggggct ctgttcccga
     7261 tggtcttatt agaacccttc ccttaccgct aaaatgttct tcgatttctt taattttatt
     7321 tacgattaca tcatcagtta aataatcttt cttttttgat gctgaaacat gagcattcac
     7381 caagacctga ggcatacttt tcatcatcgc ggaaagctct gaaagctttg catttttttg
     7441 ttttactaca cttaacagct gaatggctgt caaaagtccg tcgcctgtgg tgttgaagtc
     7501 caaaaatatt atgtgtcctg actgctctcc tcccaatgaa taaccgctct ttaacatttc
     7561 ctccaaaaca tatctgtcgc ctacgctggt ctttataata ttaataccct tatccttaag
     7621 gcatatatca agacctaagt ttgtcataac tgttccgact atggtgttgt ttctgagctt
     7681 tcctttttct ttcaagcaag tgccgcatat tgccaaaaca tggtcgccgt caacaatatt
     7741 tccgttttca tccgctgcaa tcagtctatc ggcatccccg tcgaaagaaa gtcccacgtc
     7801 agctcctgtt tctaaaacaa gcttttggat ttcttccggg tttgtagagc cgcagtctac
     7861 attaatgttt ataccgttag gctcattgtg aataacataa acttgtgctc caagctcgtg
     7921 aagaagctga ggtgcggctt tataaacagc gccgtttccg caatcgactg caattttaag
     7981 cccatttagc tccacattaa ttgtactctt taagtaatcc aaatattttc ttaatggggc
     8041 ttcaacacgt atttttcttc ctaagtctcc ggaaatcggt atgtagtcta tatccaaatt
     8101 attatttata tattcttcaa ttaagttttc tgtttcatcc gttaatttaa gtccatcctt
     8161 gtcaaaaaac tttattccgt tgtattcaat aggattgtga gatgcagtta tcatcactcc
     8221 cccgtcagca tcgagtattt taatcatgca tgcgaccgca ggtgtgggca gtattcccaa
     8281 atataaaaca tctatcccgg ctgaatttaa tcctgaagac agtgcacctt ccaacatatc
     8341 tccggatatt ctcgtatcca agccaacaac catctttggt ctttttattc cattagttaa
     8401 aaaatatcct cctactcgcc ctaatttata tgctaattca ggggacaaat ctttatttgc
     8461 aatacctctt actccatctg tgccaaatag tttacccatt cttcctccaa acaaaaaaat
     8521 ttaatcaata tccgccgtct aaaaagaggg gttatctttt tcgttatatt gtatcatatt
     8581 ttatactcta aaaaaagata ccaaaatatt aattttttat gatataatat cggtgtgaag
     8641 caagccgatt ttgcggcttg tacttatgat ataatgaaaa ctaatcattc ataaacttga
     8701 gcacttgaaa ggatacggtt atgattgttc tatcatgcaa caatgtttca aaagcttata
     8761 tggtggaaaa tataattgaa aacatttctt ttaccgttaa tgataatgaa aaggttggat
     8821 taatcggtct taacggagca ggtaaaacaa ccttgtttaa tatattgaca gaaaatctgg
     8881 agcccgattc cggcgaaata tatgttgcta agggtaagaa acttggctat cttaagcaaa
     8941 acacaattat tgaaagcaat atgagcatta tggatgaaat gcttttaatt tttaatgatg
     9001 ttatcaagat ggaagaaaaa ctccatgagc tatcggataa aattagcagt atttcgggag
     9061 aaaaaggctc tgcggaactt gaagcactaa tgaacacata ttcagaattg agcgagcagt
     9121 ttgttgaagc tgacggttac agttttagaa gcataattaa aggagtttta aaaggtcttg
     9181 gattttcaga agatgaattt gataaaccta taaacatttt aagcggcggt caaaaaagca
     9241 gagttatgct tgcacagctt ttgctcaaaa aagcagatat tcttttgctg gatgaaccta
     9301 ccaatcattt agatattgaa gcaatatctt ggcttgaaaa atatataaag aattttaaag
     9361 gtgcggtaat aattatttct cacgacagat acttccttga caacacggta aacagaattt
     9421 tccttatgga aaacaaatac ttaaaatcgt ttaacggaaa ttatacggaa tttgtgaaaa
     9481 aaagaaaaat tgagcttgaa caagcaatga aacaatatga tgaatatgaa agggaaattg
     9541 aaagacagga agaaatgatt cggcgcctcc atgcatacgg aagtaagcga aatatcagac
     9601 aggcatttag caggcaaaag gctttggata aaatcaaaaa aatggataag ccgtctgttg
     9661 gaagccaaaa attcaaatta aaatttacac cgagaatcaa tagcggcagg gatgtgttaa
     9721 gtgttaataa tgtagctgta tcttttgacg atcaacaagt tttcaaaaat gttaatttga
     9781 aaatatataa gggagaaaaa gttggaatta taggtcctaa cggtataggc aaatcaactc
     9841 tcttaaaaat tattgccaaa ataataaagg attacagcgg ggagatagaa tatggtcatt
     9901 atgtcaatat cggatattac gaccaggagc aaacaaactt aaataatgag aatactgtaa
     9961 tcgatgaaat atgggatgaa gtccctaact taaattatta tacaatccga aaatatcttg
    10021 cacaattttt gttttacgga gatgatattt ataagctcat cgaagatttg agcggcggtg
    10081 aaaaaggaag gctgtcactt ttaaaactta tgacttctaa tgcaaatttt ttgttgttgg
    10141 atgaacctac aaatcatttg gatattgatt cgaaggaagt attagaagat tcacttatta
    10201 actatgaagg aaccatattg gttgtttctc atgacagata tttcttaaac aaggttgtaa
    10261 ataagatata cgatttatcg gaagacggag catttgaata ccttggcaac tatgactact
    10321 atctggaaaa aaaagcagag cttgatgaaa cagatgatat tgatactatt acaaagacta
    10381 agacacaaat aaatgcggaa aagaaaaagg aaagggaaat tatcagggac aggcaacagc
    10441 gggaaaaaac catcaaagaa cttgaagagc aaattgatat gcatgaaaat gaaattcatg
    10501 aattagaaat caaattatgc gaaccttcga catataatga caatttttta attataaatc
    10561 tgaatgaaaa attaaacatt cgaaaaaaag agttgcaaga gctttacctt ctatgggaag
    10621 aattaatgga taactaaaaa aagttatcca ccatatgttt gtagtatgtg cataactttt
    10681 tactgtatta cttgtatcaa caactttatc cacagttgtt gataccttgt ttttataggc
    10741 tgttaattaa tttgcaagtt ctaaatttgg aattgcattt aaggatgaat ctgcaaatct
    10801 tttgtttaaa tatttataat atccggcgca gccaatcatg gctgcattat ctgtgcataa
    10861 aatcggagaa ggtctcataa atatatagtt atgcattaaa cattcttcct taaatctttc
    10921 ccttaaacgg ctgtttgacg caactccgcc ggctaatgca atagtgctta tattttcctg
    10981 ttttgcagcc ttcattgttt tcataaccaa cacgtcaacc actgcctcct gaaaacttgc
    11041 tgcaatatct tcggtattga cagtttcatt cttttgcttt gaattatgta aataattcaa
    11101 aaccgctgat tttaacccgc tgaaactaaa atccagtgtt tcatcatcaa ttatcgctct
    11161 gggaaacttg attgcatccc tctttccttt tttggctgcc atgtcaatca aaggtcctcc
    11221 cggatatccc aatccgattg ctcttgccac cttgtcaaaa gcttctccgg cagcatcatc
    11281 tctggtcatt cctaaaattt tatatacacc gtaatcctta acatgaatca ggtgagtatg
    11341 accgcccgag accacaagac agagaaatgg cggttgaagc tctttatttt caatatagtt
    11401 tgcattaata tgtccttcga tatgatttac gccaatcaag ggtttttccc atgcatagga
    11461 aagtcctttt gcaaaattaa tacccaccaa caatgctccc actaaaccgg gaccgtatgt
    11521 tacagcaatt gcatctatgt cctttggctc tgcattggct tcactcatgg cctttgatac
    11581 tacatcattt atattttcaa tatgtttcct tgaagcaacc tcaggtacaa cacctccaaa
    11641 ctccttatga gtttctattt gagatgatat tatattggat aaaacatttc tcccgttttc
    11701 aacaacagaa gcagcagttt catcacagga tgtttctatg gaaagtatta aagttttttc
    11761 gtttatattt tccttcatgc gcaatatttt cctccgtatt ttcctgaact gattatacta
    11821 ctgtgagttt cataattcaa gaagaaaaaa tgtgacagta cccccgtccc tttgtcagaa
    11881 ttcaagaaga aaaaaagaga tccttcgctg cgctcaggat gacaaagcgt cataccgaac
    11941 aatcaggaag aatctcatac taacgtgtcc ccatacctct aataaatggt aaataataaa
    12001 gatacaaagc gaacatttgc aggacacgac tataattctt agcgaatgca ggttaaaaat
    12061 ccgtttaaca gcttgcattg tttgaacgaa gtgagtttgc aagctgtagg atttttagcc
    12121 gtaatgagct ttagaattat tcgtgtccga aaccgtgagc gtgtattttt attattttta
    12181 tattttaatt agtttagagt tattttgcct tttttgtttg ttctccaaac gtggagtccc
    12241 aatgctaagc ctataacacc gtataccatg aaggttctga agtattctcc caaaagggca
    12301 ttgccaaaca gtgacaatcc tatttcagga gacattataa acattgagtt aaacaatagt
    12361 gtaccaaaaa ttgcatgttt cactcctgcc ttttgagttg aagctcctcc aaccaaaagt
    12421 gccgctacag aaaacaatcc tatttgcatg tgggcaccgt atgtgtttag ggtacccata
    12481 ttctgcaggt acattatttg tccccatgca gcaaatacag ttgacattat tgttgcaata
    12541 attctggttt tatcaacatt aatgcctgat acctcagcaa tgtgctggct ttggccgaca
    12601 cttctgaaat cctgtcccag ttttgttttc aaaatcaatt cattgaatat acataaagct
    12661 actatcaata atcccgttat taccggaacc tttctaacaa tcattaaact tgagtttgtt
    12721 gtaattgcat gaacgcttat tgcaatcata accaagctca gaataatgtt tattgctgtc
    12781 atggtcatgt tatatctgta caaggtggga ttctttttgt ttcttacgat tttccatatc
    12841 tgcaggagaa gaatacatgc aaatactgcc aatatagccc acatgaacgg aatttcccaa
    12901 attttgtcca atgcgtattt aagtccgccc ttgtcatatg cccccatgtc tactgtcatt
    12961 ctcacaccta cgccgtcagg tttaatcatg ggatgagtat tgggaacttt tattattaca
    13021 cctacaacaa acaagaataa aaattgatat ataccgtttg cgaaataacc taccatcagg
    13081 ctggcaatca tctcctgtcc tcttgttttg ttatagagca aacctgttaa atatccgaac
    13141 aaggttgcta ttgggagtgc aataagcatt gttaacaaaa gtccgttaac accgccccat
    13201 ccaaaatata atacgattgc aagtgctatt tgtcctgcca ttgcaccgac aactatacca
    13261 aagtttagcc cgagacctgc cactaccggt attatcaaag ataaaacaag aaatgcattc
    13321 ctgaagaaac ggcttgataa ttccgtcagg aaaaagttca ttgttacacc gtctgatact
    13381 aaaaatccaa atattgtgaa ggcaacgaat attaatgaaa caacattatc tgcaagccat
    13441 gttgatactt tagaattatt taatctttcg gaagtatttt cattattcat taattatcgc
    13501 ctcctttaac ctttgtcagc gcataaagaa ttattccgtt ttggataacc atacgaagta
    13561 tttcggataa atccgttcct tcaaacagtt ggtttgccac gggaagcgcg gtggtaaaaa
    13621 gaccctggta tattaatacg cctattatta cgttagatac ctttgctctt gaagcagaag
    13681 ccccgcctat taatacggca gcaaccgcgg caaatgccat cataagcggt gccgtataga
    13741 gctgtgcata gccgtaaccc tgggaataaa caataatacc gatggctccc aaaattgtag
    13801 ataaaacatt tgcttttatt ctgcatctgt caatatttat acctgaagat tgggcaaatt
    13861 ttggatttat tccacctgcc gatatggcaa ttccggattt tgacctaaaa tacagccata
    13921 ctatagtaca acatatgaaa aacattaaaa gcattcccgt tgggattatt attccggttt
    13981 caccgtttat ggtgataagc ccattttctc caaataatga gaaccttaag aaattactta
    14041 atatatgtgc tccaccgatt gcatctaact gtattgtttc tcttaaaccc tttcctataa
    14101 accatcccat attggggctt tgaaacggta tcatgagcca tgcaaggcac attgctgcaa
    14161 caatcgaaaa tcctgtgtaa gtggcgattg tcatttctga acctttaaca gcatttagaa
    14221 gtcttccgta caagtatcct acaactgtcg ccaatggtat tgcaaaagca attgctacaa
    14281 ataaccatgc ccaacctgta aatccaaatt ctatggagca gactatggca aataagccgc
    14341 atacaatacc taccggcaat gcaaagtttg gacctgttcc tgattgtatt gaaggaacca
    14401 ttgccaatac cagtataccg ttcatacccg cacgttttat cgtgtcgctt aataaagtaa
    14461 taatggatat tcccaaagct cctccggcaa taagcaagag tatccaaaaa ccgcctataa
    14521 aaattgtagg aaatcctatt ttattaacaa tatcattgaa agtggctttt ttctttttta
    14581 cttctaccat tatgcttcac tcccttctga ttttccattt cggctgcctg acatcatcaa
    14641 accaaattca gcatccggtg catcgggagg aagtactcct tcaactttcc cctcacatat
    14701 tattaaaatt ctgtcacata tgcttctcaa ctcggctaat tcggaggaag tcattattat
    14761 tgtcatgcct tcattattta attgcttgag taagtcaagc actaattttt tagcccctat
    14821 gtcgattcct ctcgtaggct cggaaactaa aagcagctcc ggtttcattg tcaatgccct
    14881 tgcaacacaa accttttgct ggttcccgcc gcttaggctt cctaccctct gcaaggagga
    14941 ggtgcatcta atatccaaat cttttatcat cttattggca tgctctctta ttttagcaga
    15001 atctttgagt ttaatgggac cataatttac taaaaattca tcgtttatct gcatggcgga
    15061 aaatgcaata ttggattcta ttgattcatc caataaaagg ccgacgcctt tcctgtcttc
    15121 gcttacaaaa gcaatgccgg atttaattgc agatttgggg ttattcagag ctgatttttt
    15181 gcccttgaat tcaacctctc cttcagctgg aaatattccc attatgccgt tggcaatacc
    15241 taatttccct tgacctgcaa gtccgcctat tcctactatt tcaccttttt ttacttttaa
    15301 gtcaacgcct tttactcttt caccgggcat tgcaacatgt aaatttttta tatctaatat
    15361 tgtttcatca acaatatttc tgttcgtaga ttcttctctt tcaatagtga tacttcttcc
    15421 gaccatcaac tgtgctattt catatttgtt tgtatcttct gtattttttg taactacatg
    15481 ctctccgtct cgcagaatgg ttactacatc cgctacttca ataatttcat caagtctgtg
    15541 gcttataaat aatatgccga ttcccttctg ggcaagcttt ttcattgctt tcaaaagatt
    15601 tgcagcttct gtttccgcta ataccgcagt gggctcatca aaaacaagga tttttaatcc
    15661 tgttttatcg atttctcttg caatttcaat aaattgcata tgaccaaccg gaaggcctgc
    15721 tacttgtaca tattcttcta tatccatgtc caacacatcc aaggctttcc tt
//
LOCUS       DAOSOW010000005        13341 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_4662_length_13341_cov_3.999022, whole genome shotgun sequence.
ACCESSION   DAOSOW010000005 DAOSOW010000000
VERSION     DAOSOW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 13341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13341
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4662_length_13341_cov_3.999022"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1146
                     /gene="msrB"
                     /locus_tag="PLD67_00390"
     CDS             178..1146
                     /gene="msrB"
                     /locus_tag="PLD67_00390"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOT21016.1"
                     /translation="MDKELKGNKLIYLAGGCFWGTEKYLGLINGVVKTEVGYANGNTE
                     NPSYEDVCRRNTGHAETVKVVYNPKVIDLETLLKLFYKAIDPVAINRQGYDIGTQYRT
                     GVYYVDKEDEAVIKESLNELQKEYKEPLAVEVTELKNYYKAEEYHQKYLEKNPGGYCH
                     IGELEFYELEKAQKNSKYKVKPKKELESTLTEMQYNVTQNNATEPPFDNEYYDNFEDG
                     IYVDITSGEPLFTSKDKFDSGCGWPSFSKPIDEKVVKEKSDLSYGMIRTEVRTSNSDS
                     HLGHVFNDGPKESGGLRYCINSAALEFIPVDKMEERGYGDLLYLFE"
     gene            1358..1876
                     /locus_tag="PLD67_00395"
     CDS             1358..1876
                     /locus_tag="PLD67_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOT21017.1"
                     /translation="MTKTKEVILCGLLLATGIILPMFFHMFGLTGPIALPMHIPVLIG
                     GFLLSPKLALLLGIITPVLSGMLTGMPVMFPMAVIMAFELGIYGLAASLAVRKFNLSV
                     IPSLIVSMIAGRIAAGLTVAILVELFGVKMNPLIYIKGAIITGIPGIIIQLIFIPALV
                     YAIKSYVKIKSV"
     gene            complement(1873..2241)
                     /locus_tag="PLD67_00400"
     CDS             complement(1873..2241)
                     /locus_tag="PLD67_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019189371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOT21018.1"
                     /translation="MYNIKIKRVYQDSSEDDGYRILVDRLWPRGISKEKINIHKWAKE
                     ITPSTELRKTFKHDPDTMDEFRVRYNFELDNNEHALDFINLLKDKLKEGNVTLLYAAK
                     NEEFNHAIVLKEWIERMISN"
     gene            complement(2291..3229)
                     /locus_tag="PLD67_00405"
     CDS             complement(2291..3229)
                     /locus_tag="PLD67_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT21019.1"
                     /translation="MNKIIQFKRKIFTRDNIGYLMIFAAGCLWGTIGLFVNLLNSAGA
                     DTTLTAFMRMFMGCLILIPIMLHKGGVSLFKIDKKGLSQCFMLGILSQALFNYSYITS
                     IRSVGVATAAILLYTAPVFVAIMSRMFFKESIGKIKILALIINIVGCFLMVTGGNLSN
                     IKLSAAGIFFGVAAGFLYSLVTIIGKSTSGDIHPFTVVFYGFLFGGIAIGIFTSPWES
                     IKTVSGLKFWIYSFGYGLIPTVGSYLLYLGGLRKQIEISKTPVIASVEIVVATLIGII
                     VFEEGLNFINMLGIIIIFSSIVIINMKTPYEGARHQ"
     gene            complement(3226..3885)
                     /locus_tag="PLD67_00410"
     CDS             complement(3226..3885)
                     /locus_tag="PLD67_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21020.1"
                     /translation="MARRKRVYSRRRRINYGRVAFLIAVLVVIILLVMKIFSKNRYIS
                     KVEEALEKDITRIAGSISTVSKIDATIYENEGIKYTNQHENIKRIMTFDGSLSEDMDK
                     AGDVKILLENLLISEKTEVTAELPPKDDGYYWIEADIFTKDKVLIFSVEEEYNFDLYY
                     DIENKTVYVKEKYYDEFSKKYKKVKFQGYSVTDEFRNIINSMANTGLDTSGTQIPETD
                     Q"
     gene            complement(3887..4504)
                     /gene="coaE"
                     /locus_tag="PLD67_00415"
     CDS             complement(3887..4504)
                     /gene="coaE"
                     /locus_tag="PLD67_00415"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOT21021.1"
                     /translation="MKQNRPKVIVITGSIGTGKSTAVDIIKEFGFTVLDSDKIVHEGY
                     NIGSELYYKIINHFGEDILKGDEIDRQKLGEIVFNNDKKLNELNNMVHGYVYDKLKEG
                     IEASKEKVIFLDIPLLFETKDIHEPIYDEIWLIYVSEETQRKRLMERAIAENKKPEDV
                     LKIINKQISIKEKVFMADVVINNEGTVEELKENIKALLEIRGMGW"
     gene            complement(4491..7190)
                     /gene="polA"
                     /locus_tag="PLD67_00420"
     CDS             complement(4491..7190)
                     /gene="polA"
                     /locus_tag="PLD67_00420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004823983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOT21022.1"
                     /translation="MKKIMILDGNSLLFRAFYALPPLKTKKGQYTNAVYGFLSMLYKI
                     IDTYSPEYLCVAFDPEKPTFRHEKYKDYKANRQKAPNELVQQFKLIRDVLDVHNIKRV
                     EVEGFEADDVSGTFACAAKEQGADVYLVTSDKDYLQLIDENVRVILTKKGITNIVEMD
                     INSMGEEYGLKPEQFVDLKALMGDSSDNIPGVSGVGEKTALKLIQEYKSLDNLYENIN
                     KITGKLKEKLETDKMQAYMSQTLARIITDIPIEFNIEEFKIKKPDIKKLSELYDELEF
                     RQFKKRLVETIPVDDNGQISLFDTDRAETVKNIDIIYIDSEEKLTEIIKNISRKEKAI
                     LKFLLDGERALFSNALALGISDGENIYYIDFEKAEEKKVLNELKEIFESEKIAIWGHN
                     LKNEIIYLMKNNIQINNIQFDSEIGKYLLNPSESSYSLDKIAYEFLNVEIPSENQILG
                     TGVKRKTFKEIDIEQRKAYIFNYLNTIGKVQKQITEDIDELKMTCLYEEIELPLIEVL
                     AYMEFVGFKVDISVIDKLGVHFQEKIAELEKDIYNIAGESFNINSPKQLSVILFDKLG
                     LPIIKKTKTGISTDIEVLERLSSEHAIVDKIIEYRQMVKLNSTYVEGLKNEVNKKTGR
                     VHSIFNQTIASTGRISSTEPNLQNIPTRTEEGRELRKAFVPEDDYILCDADYSQIELR
                     VLSHLANEQNLIDAFEQGLDIHAKTASQVFHVKLSEVTSEMRGRAKAVNFGIVYGISD
                     YGLARDLKIPRKEAKKYIDNYMKYYSNIEKYMKDIVEQGKRDGYVTTYFGRRRYIPEL
                     TSRNFNIRSFGERIALNTPVQGTAADIIKAAMVGVYRRLKNNKMKSRLILQVHDELII
                     EAYKDELEDVKTILSEEMENVVVDFKVRLKSDINTGSSWYEAK"
     gene            7332..7919
                     /locus_tag="PLD67_00425"
     CDS             7332..7919
                     /locus_tag="PLD67_00425"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HOT21023.1"
                     /translation="MKLEPKNYNFSNVRYFSPIQLSEHYQLYINYISCLNKVNNDLKN
                     DLIYDECNSNYSDIRSAQKSKTFCFDSIKLHELYFENLTGNNTRIHGPIERVLVNTFG
                     SYNNFREKFKCIGMSMRGWVLLCYDRYNNEFYIYGQDSHDCGVMLYAEPLIVMDVYEH
                     AYMIDFGTNRNVYIDAFFQNIDFNVINQRLNKILK"
     gene            complement(8156..8773)
                     /locus_tag="PLD67_00430"
     CDS             complement(8156..8773)
                     /locus_tag="PLD67_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOT21024.1"
                     /translation="MKNLTINEFAKIVASDSPVPGGGSIAALCGALSAALSEMVANLT
                     VTKKKYADSKEEMNAIINKASQLRDRLLNYIEEDSLAYKKVMEAYKLPKETEEEKCLR
                     LERIQEGLKVAASVPLEVAETSYEIFPLVEAVVLRGNSSSVTDALVGAMMARTGVLSA
                     ILNIRINLDSIKDEDFVKVLKEKADILEGKTRQYEKKILELAPFK"
     gene            complement(8770..10017)
                     /gene="hutI"
                     /locus_tag="PLD67_00435"
     CDS             complement(8770..10017)
                     /gene="hutI"
                     /locus_tag="PLD67_00435"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOT21025.1"
                     /translation="MSKLIIKNASELVTCKGKAPKHGKDMSDIGLIENGCLVIEDDII
                     VDVGTSNILKNYDEDDYQIIDAKDKAVLPGFIDSHTHLIFGGYRADEFSWRLKGDSYM
                     SIMERGGGITSSVRATRNTSLEELTALGKTRLDKILAMGVTTVEGKSGYGLDCETEIR
                     QLKAMKQLNDMHPVDIVPTFLGPHSVLPEYKGKEDEFIQFMIDDVLPKVKEENLAEFA
                     DIFCEKNVFTIEQSRKFLTAAKNSGLKLKIHADEMYQLGGAELAVELGCTSADHLLQA
                     SDEGIRQMSKSNTVATLLPGTAFCLKEEYARARYMIDSGCAVSLATDFNPGSCFTNSI
                     PLIIALAALHMNMTIEEIITSLTINAAAALGRSDTRGSIEKGKKADIIILEYPSIHFL
                     PYHVAVNIVETVIKNGKIVRGLL"
     gene            complement(10092..10982)
                     /gene="ftcD"
                     /locus_tag="PLD67_00440"
     CDS             complement(10092..10982)
                     /gene="ftcD"
                     /locus_tag="PLD67_00440"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005833514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOT21026.1"
                     /translation="MDRIIECVPNFSEGRNTEKIEKILDVFRGRKGVKLLDYSSDENH
                     NRTVVTVVGEPEEIKKVMVEAIGKAVELIDLNYHQGQHPRMGAVDVVPFIPVRNVTVE
                     EADKLAKAVAKEAADKYNLPFFLYEKSASAPHRENLANVRKGQFEGMKEKMKDELWKP
                     DFGPDTIHPTAGVTAIGARMPLIAFNVNLSTPDIEVANRIAKQVRHISGGFRYVKAIG
                     VEIHDRGLVQVSMNLTDYTKTSIYRVFETIKMEAARYGVNVVGSEIVGLIPMQALVDT
                     AEYYLRLENFKYDQVLETRL"
     gene            complement(10975..13017)
                     /locus_tag="PLD67_00445"
     CDS             complement(10975..13017)
                     /locus_tag="PLD67_00445"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOT21027.1"
                     /translation="MTDNLNIFNAMKIKLSDELPEYPVLKENVRRAPKREVTLNEREI
                     KLALKNALRYVPGSLHEKLAPEFLDELMTYGRIYGYRFMPKERIYGKPIDEYKGKCIE
                     GKAFQVMIDNNLDHEVALYPYELVTYGETGQVCQNWMQYQLIKKYLEALTEEQTLALM
                     SGHPLGLFKSHKNSPRVIISNGLMVGMFDNPQDWAKATAMGCSSYGQMTAGGWMYIGP
                     QGIVHGTFNTLINAGRKELGIPDDKDLAGHLFITSGLGGMSGAQAKAVEIANGVGIIA
                     EVDYSRIETRLKQGWVSTVTDNLEEAFEIAREYLYKKQTISIAYHGNIVDLLEYAADN
                     NIHVDLLSDQTSCHVPYDGGYCPKGLTFEERTELLNSNREKFVQMVNKSLADHFRAIK
                     TLVDRGSYFFDYGNAFMKAVFDAGVKEIAKNGNDTSEGFIFPSYVEDIMGPMLFDYGY
                     GPFRWVCLSGKPEDLHKTDQAAMSVIDPNRRGQDRDNYIWIRDAEKNKLVVGTQARIL
                     YQDALGRRDIALKFNDMVRKGEIGPVMLGRDHHDTGGTDSPFRETSNIYDGSNVTADM
                     AVHCFAGNAARGMSLVALHNGGGVGVSKAINGGFGLVLDGSKRVDEIIKTSIPWDTMV
                     GVSRRSWARNEHSIETTIEYNKKFEGEDHITIPFLAKDELIEETLKNYKNKGERNG"
     gene            complement(13157..>13341)
                     /locus_tag="PLD67_00450"
     CDS             complement(13157..>13341)
                     /locus_tag="PLD67_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21028.1"
                     /translation="PMINQRFASWIKDKYPHLIYINREEDMGSEGLRKAKLSYYPDKM
                     AIKYRAVTKKNYALHK"
BASE COUNT         3819 a         2688 c         2142 g         4692 t
ORIGIN      
        1 gtatagagta agtatacatt aattgattat actaatggaa aggttcctat gaataaaaaa
       61 ttgattttac tgttttttgc cttaatattg gtaattgttt tgtttgcttg tgataataat
      121 aagaagagtt acaatatgac agcaaataat gataatttaa gaaaggatga tgaacttatg
      181 gataaagaat taaagggcaa taaattgatt taccttgcag gaggatgttt ttggggaaca
      241 gaaaaatatt taggcttaat caacggagta gttaagactg aggtaggata tgcaaacgga
      301 aatacagaaa atccttctta tgaggatgtc tgcaggagaa ataccggtca tgcggaaact
      361 gttaaagtgg tttataaccc gaaagtgatt gatttagaaa ctttacttaa gttattctac
      421 aaagcaattg acccggtagc tataaacaga caaggctatg atatagggac tcaatatcga
      481 acaggcgtgt attatgttga caaagaggat gaagctgtaa ttaaagaatc attgaatgaa
      541 ttgcaaaagg aatataagga gcccttggct gttgaagtga cggaacttaa gaattattac
      601 aaagcagaag aatatcacca aaaatattta gaaaagaatc ctggcggata ctgccatata
      661 ggtgaattgg aattttacga gttggagaaa gctcaaaaaa attcaaagta taaggtaaaa
      721 ccaaaaaagg aattagagtc aacactgacg gaaatgcagt acaatgttac tcaaaacaat
      781 gctactgagc cgccttttga taatgaatat tacgataatt ttgaagatgg tatatatgtg
      841 gatataactt ccggtgaacc tttgtttaca tctaaggata aatttgattc aggatgtggg
      901 tggccaagtt tctcaaagcc cattgatgaa aaagttgtta aagagaaaag cgatttaagt
      961 tacggcatga taagaacaga agtaagaacg tcaaacagtg attcgcattt agggcatgtt
     1021 tttaatgatg gtcccaaaga atcgggcgga ttaagatact gtatcaacag tgctgcattg
     1081 gaattcattc ctgtagataa aatggaagaa agaggatatg gagacttgct ttatttattt
     1141 gaataaataa attcgctgtg tcatcctgag cctaagcgaa ggatctcagg ttttataaaa
     1201 tgagattctt cgagctaatg ctctcaaaat gacacgggct tatagatagc aatgacaaaa
     1261 atgttaaaat attgtattgc atattatgac aaaattttat atgataacat acatatcata
     1321 ataaattttg cagctataaa tggagggtaa aaataaattg actaaaacaa aagaagttat
     1381 attatgcgga ttattactgg caacaggtat aatactaccc atgttttttc atatgtttgg
     1441 tttgacaggt cctattgctt taccgatgca tatacctgta ctaatcggag gttttttatt
     1501 gtcgccaaag cttgcattgc tcttaggtat aataacaccg gtcttgagcg gaatgttaac
     1561 aggaatgcct gtaatgttcc ctatggctgt aataatggct ttcgaattag gaatatatgg
     1621 tttagcagca tcattagctg taagaaaatt taacttatca gttatacctt cattaattgt
     1681 ttccatgata gcaggaagaa tagcagccgg tttaacagtt gcaatcttag ttgaattatt
     1741 cggagtaaaa atgaaccccc tcatttatat caagggggca ataataacag gtattcccgg
     1801 tatcataata caattaatct ttataccggc attagtttat gccataaaat cttatgtgaa
     1861 aattaaatcg gtctagttgg aaatcatcct ttcaatccat tccttcaaaa caatggcatg
     1921 attgaattct tcatttttgg cagcatacaa caatgtaaca tttccttctt ttaatttgtc
     1981 ctttaataaa ttgataaaat caagggcatg ttcattgtta tccagctcaa aattatatct
     2041 taccctgaat tcatccattg tatccgggtc atgtttaaac gtcttcctta gctcagtgct
     2101 gggagtgatt tcctttgccc atttatgaat atttattttt tctttgctta ttcctcttgg
     2161 ccaaagacgg tctaccaata ttctgtaacc gtcatcttca gaggagtctt gatatactct
     2221 tttaatctta atattataca tatcttttct ccttttactt cattattcat tggtgcctgg
     2281 cacctttggc ttattggtgc ctggcacctt cgtatggcgt cttcatattt ataatgacaa
     2341 tggaagaaaa tattatgata attccaagca tatttataaa atttaatcct tcttcgaaca
     2401 caataattcc aatcagtgta gcaaccacta tctctactga tgcaattaca ggagtctttg
     2461 agatttctat ttgctttctc aaccctccca agtataacaa atatgaccct acggttggta
     2521 ttagaccata cccgaacgaa tagatccaaa atttcagtcc ggatacggtt ttaatagact
     2581 cccaaggtga agtaaatatt ccaatggcaa ttccgccaaa taaaaagccg taaaaaacaa
     2641 ccgtaaaagg atgaatatca cccgaggtag actttcctat tatagttacc aagctataca
     2701 aaaaacctgc tgccacgcca aaaaatattc cggcggccga taattttata tttgacagat
     2761 tcccgcccgt aaccattaaa aaacatccga caatatttat aattaatgca agaattttga
     2821 tttttcctat tgattcctta aaaaacattc ttgacatgat ggcgacaaaa acaggtgccg
     2881 tatataaaag tatggctgca gtggcaactc ctacgcttcg aatacttgtg atataactgt
     2941 aattaaaaag agcctgggac aatataccta acataaagca ttggctcaac ccctttttgt
     3001 caattttaaa caagcttacg ccgcccttgt gaagcattat ggggatgagt atcaaacatc
     3061 ccataaacat cctcataaat gccgttaatg tggtgtcagc gccggcactg tttaataaat
     3121 taacaaacag tcctattgta ccccacaagc aacctgctgc aaaaatcatc aaatagccga
     3181 tattgtcgcg cgtaaaaatt ttcctcttaa attgaattat tttattcatt ggtcagtctc
     3241 cggtatttgc gtcccggaag tgtctagacc ggtatttgcc atactattaa tgatatttct
     3301 gaattcatct gttacgctgt aaccttggaa tttgactttt ttatattttt tgctgaattc
     3361 atcgtagtat ttttctttta catatacagt cttgttttca atatcataat acaaatcaaa
     3421 gttgtattct tcttctacac tgaaaatcaa tactttgtct tttgtaaaaa tatcagcttc
     3481 tatccaataa tagccgtcat cctttggggg cagttcagct gttacttctg ttttttcgga
     3541 gataagcaga ttttcaagca aaatttttac gtcgccggcc ttgtccatat cttctgacag
     3601 acttccgtcg aaagtcataa tcctttttat attttcatgc tgattggtat atttaattcc
     3661 ttcattttca taaatagttg catcaatttt ggaaacggtg ctgattgaac cggcaattcg
     3721 agtaatatct ttctccaatg cttcttcaac ctttgaaata tatctgtttt tactgaatat
     3781 tttcattact agcagtataa taacaacaag taccgcaatt aaaaaagcaa ctcgtccgta
     3841 attaattctt cttcttctgc tgtacactct ttttctcctg gccatttcac caccccatac
     3901 ctcttatttc aagcagagct tttatattct cctttagctc ttcaacagtg ccttcgttat
     3961 ttataactac atcggccata aatacttttt ctttaataga tatttgctta tttataattt
     4021 ttagtacatc ttcgggtttt ttgttttctg caatggctct ttccataagt ctttttcttt
     4081 gagtttcctc ggaaacatat atcaaccata tttcgtcgta tatgggctca tgtatatctt
     4141 tggtttcaaa tagcagtggt atgtctagaa atattacctt ttctttgctt gcctcaatac
     4201 cttcttttaa tttgtcatat acatagccgt gcaccatatt attaagttca tttaattttt
     4261 tatcgttgtt aaaaacgatt tctccaagct tttgcctgtc tatttcatca ccttttaata
     4321 tatcctcgcc aaaatgattg ataattttgt aatagagctc acttccaata ttgtatcctt
     4381 catggacaat tttatccgaa tcaagaaccg tgaacccaaa ctccttaatt atatcaacag
     4441 ctgtagattt tcctgttcca atacttccgg taatcactat gaccttaggt ctattttgct
     4501 tcataccagc tactccccgt attaatatca gactttaagc gaaccttaaa atcaacaact
     4561 acattttcca tttcttctga aagaattgtc tttacatctt caagttcatc tttgtatgct
     4621 tcaataataa gttcgtcatg aacctgcagg ataagcctcg acttcatctt gttattttta
     4681 agccttcggt aaactccgac catggctgcc tttataatat cagcagcggt tccttggacg
     4741 ggagtgttta gagcaattct ttctccaaag gagcgtatgt tgaaattacg tgatgtaagc
     4801 tcgggaatat accttctcct tccaaaatat gtagttacat aaccgtccct tttaccctgc
     4861 tccacaatat ccttcatgta tttttctata tttgaatagt atttcatata attatctata
     4921 tatttttttg cttcttttct cgggattttt aaatctctgg caagtccgta atcacttatg
     4981 ccgtacacta ttccaaagtt tacggctttg gctcttcctc tcatctctga agttacttcg
     5041 gataatttaa catggaaaac ctgagaggca gtctttgcat gaatatctaa cccttgctcg
     5101 aatgcatcta ttaaattctg ctcatttgcc aagtgggaca gtactctaag ctcaatttgc
     5161 gagtagtcgg catcgcaaag tatataatca tcctccggca caaatgcttt tctcagctct
     5221 cttccttctt cggtgcgggt gggaatattc tgaaggtttg gctctgtact tgatattctt
     5281 ccggttgaag caattgtttg attaaatatt gaatgcaccc tgcccgtttt cttgttcact
     5341 tcgtttttca atccttccac ataagtggag tttagtttaa ccatttgcct gtattcaata
     5401 attttatcaa ctattgcatg ctctgagctc agtctttcaa gcacttcaat atcggttgaa
     5461 ataccggtct ttgttttctt tattatcggc agacctaatt tatcaaacaa aataacggaa
     5521 agttgttttg gagaattaat attgaagctc tcacctgcaa tattataaat atccttttca
     5581 agctccgcga ttttttcctg aaaatgaact cctaatttat caataacgct tatatcgact
     5641 ttaaagccca caaattccat gtaagccaaa acttcaatca atggaagctc aatttcttca
     5701 taaagacagg tcatcttaag ctcgtctata tcctcggtta tttgtttttg tactttgcct
     5761 atggtgttta agtaattgaa tatatatgcc ttcctttgtt ctatgtcaat ttccttgaat
     5821 gtttttcttt taactcccgt accaagaatt tgattttctg aaggaatttc cacattcaag
     5881 aattcgtagg caatcttgtc caatgaatag gagctttctg aagggttcaa aaggtatttt
     5941 cctatttccg aatcaaattg gatattattt atttgaatat tgtttttcat taaatagata
     6001 atttcatttt ttaaattatg tccccaaatt gcgatttttt cgctttcaaa tatttctttt
     6061 aattcattaa gaactttttt ttcttctgct ttttcaaaat ctatataata aatattttca
     6121 ccgtcggaaa tacctaaggc aagagcattg ctaaacagtg ctctttctcc gtcaagcaaa
     6181 aattttaata tcgctttctc tttgcggctt atatttttga ttatttctgt taatttttct
     6241 tctgagtcaa tataaataat gtcaatattt ttaactgttt cagccctgtc ggtatcaaac
     6301 aatgatattt gcccattatc atcaacagga atagtttcaa caagacgctt tttaaactgg
     6361 cggaattcca gttcatcata taattctgac agtttcttaa tatcaggttt cttaattttg
     6421 aattcctcta tattaaattc aatcggaata tccgtaataa tacgcgccaa ggtctggctc
     6481 atataagcct gcattttatc cgtttccagt ttttctttaa gcttgcctgt aatcttgtta
     6541 atgttttcat ataaattatc taagctttta tactcctgga taagcttaag agctgtcttt
     6601 tcgcctactc ccgatacacc gggtatattg tcggaggaat ctcccatgag agcctttaaa
     6661 tcaacaaatt gttcaggttt taacccgtac tcttctccca tggagtttat atccatttcc
     6721 acaatatttg taataccttt tttagttaaa attactctta cattttcatc tatgagctgc
     6781 aggtagtcct tgtcactggt aaccaggtaa acatctgctc cctgctcctt tgcagcgcat
     6841 gcaaatgtac cggatacatc atctgcttcg aagccttcaa cctctaccct ttttatatta
     6901 tgtacatcaa gaacatccct tataagttta aactgctgaa ccaattcatt tggtgctttt
     6961 tgacgatttg ctttgtaatc tttatatttt tcgtgcctga atgtcggttt ttcagggtca
     7021 aaagcaacac ataaatattc aggggaatat gtatctataa ttttatacaa catgctcaaa
     7081 aaaccgtata cagcatttgt atattgtcct ttttttgtct ttaggggcgg taatgcgtaa
     7141 aatgctctga acagcaagct gttgccgtcc aatatcatta tttttttcat ggcatctcct
     7201 ttatgaattg acccgatttc ttgtgttttc tttattatat caacaaaaac catgcgggta
     7261 aaggaaaact ttcagttcat atattcattt tcccggacat ataattaaat atatttatcg
     7321 gaggtataaa aatgaaactg gagccaaaaa attataactt ttcaaatgta agatattttt
     7381 cgccgattca attaagcgag cattatcagc tatacataaa ttacatctct tgccttaaca
     7441 aggtaaacaa cgatttgaaa aatgatctaa tatatgatga atgcaattcc aactacagcg
     7501 acattcgctc tgcccaaaaa tctaaaacct tttgttttga cagcataaag cttcatgagt
     7561 tatattttga aaatttaaca gggaacaata ccagaattca cggtcctatc gaaagagtac
     7621 tggttaatac tttcggttcc tacaataatt tccgcgaaaa gttcaaatgc atcggtatga
     7681 gtatgagagg atgggttttg ctttgttatg acaggtataa caatgaattc tatatatacg
     7741 gtcaagactc ccatgattgc ggtgtaatgc tttatgctga gcctttaatt gtaatggatg
     7801 tttatgagca tgcatatatg attgattttg gaacaaaccg aaatgtatat attgatgctt
     7861 ttttccaaaa cattgatttt aatgtaatta atcaacggtt aaataaaatt ttgaaataat
     7921 cacagtaact aaagaaacct tcagacatat aatatcatac acacataaat ttaaggaggt
     7981 taattatggg attttttcat agaggtagag tagccggagc aggcggttca aatttactat
     8041 ttttcttctt attattagtt atactgtttg acgagtgctt tgatgacatg aagtgctagt
     8101 aaccctcggg acagctacat aagctgtccc gttctagttg gtgcatactg tgtcattatt
     8161 tgaatggcgc taattctaat atttttttct catattgtct tgtttttcct tcaagtatat
     8221 ctgccttttc ttttaatact tttacaaagt cttcgtcctt aattgaatcc aaattaattc
     8281 taatatttaa tatggctgac agcactccgg ttcttgccat cattgcacca accaaggcat
     8341 ccgtaacaga acttgaattt cctcttaaca ctactgcttc caccaaagga aatatttcat
     8401 atgaagtttc cgcaacctct aatggtaccg atgctgcaac ttttaatcct tcttgaattc
     8461 tctcgaggcg aaggcatttt tcttcttctg tttcttttgg aagcttgtat gcttccataa
     8521 ccttcttgta tgcaaggctg tcttcttcga tatagtttag aagcctatct ctaagctgag
     8581 aagctttatt gattatagca ttcatctctt cttttgaatc agcatacttt ttctttgtaa
     8641 cagttaaatt tgcaaccatt tcggaaagtg cagcgcttaa tgctccgcac aatgccgcaa
     8701 tacttcctcc tccgggcacg ggtgaatcag aagcaactat ttttgcaaat tcgtttattg
     8761 tcaaattttt cataataacc ctcttacgat tttaccgttt tttattacag tttcaacaat
     8821 atttactgca acgtggtatg gaagaaagtg aattgaagga tattccaata taataatatc
     8881 cgccttctta cccttttcta tacttcctct tgtatcactt ctgcctaatg cagctgcagc
     8941 atttatagtc agtgaagtta taatttcttc aattgtcata ttcatatgaa gagcagcaag
     9001 agctataata agcggaatgg agtttgtaaa acagctcccc gggttgaagt ccgtagccaa
     9061 agaaacggca catcccgaat caatcatgta tcttgctctt gcatattctt ccttaagaca
     9121 aaatgccgtt cccggaagaa gggttgcaac tgtattagac ttagacatct gtcttattcc
     9181 ttcatctgaa gcctgcagca aatggtctgc cgaagtgcag cctaactcaa cggctaattc
     9241 agctcctccc aactgataca tttcatctgc gtgaattttt aattttaacc ctgaattctt
     9301 tgcggcagtc aggaattttc gggactgctc tattgtaaat acatttttct cgcaaaatat
     9361 atctgcaaat tcagctaagt tttcttcctt aaccttaggc aggacatcat ctatcataaa
     9421 ttgaataaat tcatcttcct tgcccttata ttccggaaga acgctgtgag gtcccaaaaa
     9481 tgtaggaaca atatcaacag gatgcatatc gtttaattgt ttcattgctt taagctgtct
     9541 tatttccgtt tcacaatcaa gtccgtatcc gctttttccc tccaccgttg taacgcccat
     9601 agctaatatt ttatcaagtc tggttttccc cagagctgtt aattcttcta aggaggtgtt
     9661 ccttgtagct cttacggagc ttgttattcc tcctccgcgc tccattattg acatatagct
     9721 gtcgcccttt aatctccatg aaaattcatc ggctctgtag cctccgaaaa ttaaatgtgt
     9781 gtgggaatct ataaatccgg gcaatacggc tttatctttt gcatcaataa tttgatagtc
     9841 atcttcatca taatttttta atatgttcga ggttccaaca tcaactatta tatcgtcttc
     9901 tattacaagg catccgtttt caatcagccc tatatcagac atatccttgc cgtgtttggg
     9961 ggcttttccc ttacatgtaa caagctcgga tgcatttttt ataatcaatt tactcattat
    10021 tacctccttt atgacaaggg gacgggggtg ttgtcatcaa atgtccacca agaaccgtcc
    10081 cccctggtca tttataaccg ggtttcgaga acttggtcgt atttaaagtt ttccaggcgc
    10141 agatagtatt ctgccgtatc caccaaggcc tgcattggaa tcaacccaac aatttcgctt
    10201 ccgacaacat ttacaccata ccttgccgct tccattttga tagtttcaaa tacacggtat
    10261 atgcttgttt ttgtataatc ggttaagttc attgatactt gtacaagacc tctgtcatgt
    10321 atttcaacgc ctatagcttt tacatatcta aatcctccgc ttatatgtct tacctgcttt
    10381 gcaattctat ttgcaacttc tatatcagga gttgataaat taacattgaa agcgatcaga
    10441 ggcattcttg caccaattgc cgttacgcct gcagtcggat ggatagtatc aggtccaaag
    10501 tccggcttcc aaagctcatc cttcattttt tctttcattc cttcaaactg tccttttctc
    10561 acattggcaa gattctctct gtggggtgct gatgcagact tctcataaag gaaaaaggga
    10621 agattatatt tatcggctgc ttccttagct acagcctttg ctagtttatc ggcttcttca
    10681 acagtaacat ttcttacggg gatgaaagga accacatcta ccgctcccat tcggggatgc
    10741 tgtccctggt gataattcag gtcaattagc tctactgcct tgccgatagc ttcaaccata
    10801 acttttttaa tctcctcagg ctctcccaca acggttacca ctgtacggtt gtggttttca
    10861 tcgcttgaat agtcaagaag cttgactcct tttcttcctc tgaaaacatc cagaattttt
    10921 tctatttttt ctgtatttct gccctcactg aaattaggga cacattcaat tattctatcc
    10981 atttcgctct cccttatttt tgtaattttt taaggtttct tcaattaatt catcttttgc
    11041 caaaaatggt attgtaatgt ggtcctctcc ttcaaatttt ttgttgtatt caatcgttgt
    11101 ttctattgaa tgctcgtttc ttgcccagct tcttcttgac accccgacca ttgtatccca
    11161 aggaatggaa gtttttatta tttcatcgac tcttttgctt ccgtcaagaa caagaccgaa
    11221 ccccccgtta atcgccttgc tgacccctac accaccgccg ttgtgaagtg caaccaagga
    11281 catacccctt gctgcatttc ccgcaaagca gtgaaccgcc atatctgccg taacattgct
    11341 gccgtcatat atgtttgatg tttccctgaa aggagaatcc gttccgcctg tatcatgatg
    11401 gtctctcccc agcattacag gaccgatttc cccttttctt accatatcat taaatttaag
    11461 tgctatgtca cgtcttccca atgcatcttg ataaagaatt cttgcttgag ttcccacaac
    11521 taatttattt ttctctgcat ctctaatcca aatataatta tctctgtcct gacctcttct
    11581 gttagggtct atcacagaca ttgcagcttg gtctgtttta tgtaaatctt caggctttcc
    11641 gcttaaacaa acccacctga agggtccgta tccgtaatca aaaagcatgg gtcccattat
    11701 atcttccaca taggaaggaa atataaatcc ttcgcttgta tcattgccgt tcttggcaat
    11761 ttccttcact ccagcatcaa atacagcctt catgaatgca ttgccgtaat cgaagaaata
    11821 tgagcctctg tctaccaaag tttttattgc tctaaagtgg tcagccaatg acttatttac
    11881 catctgtaca aatttttctc tgttgctgtt taataattca gttctttctt caaaggttag
    11941 tcctttggga caataacctc cgtcatacgg tacatggcat gaagtctggt cggacaacaa
    12001 atccacatga atgttgttat ctgctgcata ttccaacaag tccacaatgt tgccgtggta
    12061 tgctattgaa attgtttgtt ttttgtatag gtactcccgt gcaatttcaa atgcttcctc
    12121 aagattatcg gtaaccgtgg aaacccatcc ctgttttaac ctggtttcaa ttcttgagta
    12181 gtcaacctct gcaattatac ctacgccgtt ggctatttca acagctttag cctgtgcgcc
    12241 gctcatccct ccaagtcctg aggttatgaa taaatgtcct gccaaatctt tatcatcagg
    12301 gataccgagt tcttttctgc ctgcatttat aagtgtatta aacgttccgt gaacaatacc
    12361 ctgaggtcct atgtacatcc agccgcctgc cgtcatttgc ccatagcttg aacatcccat
    12421 tgcggttgcc ttcgcccaat cctgcggatt gtcaaacatt cctaccataa gtccgtttga
    12481 tattataact ctggggctgt ttttatggga tttaaacaat cccaacggat gaccggacat
    12541 aagggctaat gtttgctctt ccgttaatgc ttcaagatat ttttttatta attgatactg
    12601 catccaattt tggcaaacct gacctgtttc cccgtaggta accaattcat acgggtaaag
    12661 ggcaacttca tggtctaaat tattgtcaat cataacttgg aatgctttgc cttctataca
    12721 tttccccttg tactcatcta taggcttacc gtaaattctt tctttaggca tgaaacggta
    12781 gccgtaaatc cttccatatg tcatcaattc atccaaaaat tcgggagcta atttttcatg
    12841 gagacttccc ggcacatacc ttaatgcatt cttcaatgcc agctttattt ctctttcatt
    12901 taaagtaact tctcttttgg gagcacgtct tacattttcc tttaaaaccg gatactctgg
    12961 taattcatcg cttaacttga ttttcattgc attaaaaata tttaaattat cagtcatatt
    13021 acacctccta aaacaaatta aaaaaaattt tatatcttaa gattacttaa agctttttcg
    13081 ggtaaactat atataagtta aatagtaaat ttaacttccc tctccccctt gtttttttat
    13141 aaacggttat tatgccttac ttatgtaagg cataattttt tttagtcaca gcgcgatatt
    13201 taatcgccat cttgtcagga taatatgaaa gttttgcttt ccttaagcct tcagagccca
    13261 tgtcttcttc tctgtttatg taaatcaaat gaggatattt atctttaatc caggaagcaa
    13321 atcgttggtt tatcatcgga t
//
LOCUS       DAOSOW010000006        12357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_5112_length_12357_cov_4.276215, whole genome shotgun sequence.
ACCESSION   DAOSOW010000006 DAOSOW010000000
VERSION     DAOSOW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 12357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12357
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5112_length_12357_cov_4.276215"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1580
                     /locus_tag="PLD67_00455"
     CDS             159..1580
                     /locus_tag="PLD67_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT21029.1"
                     /translation="MDTYQIISIIGVIIFIYFIFFKNRRSAQKEISSLNEIEYIGSST
                     MKFDDVAGNYEAKQSLLELVDFIQNPEKYSKFNARMPKGVLLYGEPGTGKTLMAKALA
                     GEAGVPFYAVSGSDFVQVYAGLGAGRIRNLFKKAKKSGKSVIFIDEIDAIGKKRMSGS
                     IKGSDEGDRTLNALLTEMSGFADDSGTVVIAATNRIDTLDSALLRPGRFDRQIEIMLP
                     DKNARKDIIKLYLDKKNAEDINIDELADLTVYFSGAMIENLINEAALLAIRQNSDKIK
                     EEHIKKAYLQILAGVEKEDYDNGFQKTITSYHEAGHAFVSRHLLPENKIIRVSVIPTS
                     KGAGGYTLSTPDSEEVMTKGKMINHLSVLMAGRASEEVFFGNENITAGAQNDIEKVTS
                     LAVDYVSKYGMNDATFINYQLLSDKLRIPLTSVEVSVKKLTSEIYEEVIRLIECNKEK
                     VEKIARLLMEKEVIYQDELDNLI"
     gene            1690..1863
                     /gene="ssrS"
                     /locus_tag="PLD67_00460"
     ncRNA           1690..1863
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLD67_00460"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            2072..3073
                     /locus_tag="PLD67_00465"
     CDS             2072..3073
                     /locus_tag="PLD67_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOT21030.1"
                     /translation="MITIKDVARLAEVSISTVSRVINDSKPVSPEVRKRVLKVIEETG
                     YKPNDVARSLVTRRSYLIGVIVNNLANSYVADTVRGIEEIGKMYGYDILLCSSYGSKE
                     TQEKYLQLLDRKQAEGLFLVGSIFDEEIIKLAKSFNKPCVFFTRDIRENMNYITIDCK
                     SAIYEVTKYLFEEGHRKIAYFSDYENRTTVEDEKVEGYLKAIEDNEMEYSKIYVVGGS
                     KHNEAYDLGKSIIDEAKDFTACVCSSDEIAIGILDSFLDNGIKVPDDVSIVGFGNIRE
                     GKFVRPELTTIGEPYYDVGAVGMRMLIKMIKGDKIQQGRMELPFTIEKRKSVKNLNK"
     gene            3191..3655
                     /locus_tag="PLD67_00470"
     CDS             3191..3655
                     /locus_tag="PLD67_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21031.1"
                     /translation="MKKRFIFTMIVLLLFSYNSINGYEYHKEIETIEYLLNERITIMN
                     EFLYGYKDMQSLEEKLSKIESDNLLQNDLDILYKVKNSPTDYELAMKVKVNEIYDIEL
                     TDDGYLVNADLNWLMRGYDGEFNLIRNYQIKCIEDGGQSYLAKLTVLNNTAE"
     gene            3836..4318
                     /locus_tag="PLD67_00475"
     CDS             3836..4318
                     /locus_tag="PLD67_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOT21032.1"
                     /translation="MQNEFLQSKKSEFAELKEYIDSVKNSQGVTMQILQKAQSLFGYL
                     PLEVQQFISDETNIPLADIYGVVTFYTQFSTEEKGKHKIGLCLGTACYVKGSQGILDK
                     ISDELNVKVGKTTDDKLFTLEATRCLGCCGLAPVMMVDDDVYGKLDTTKIPGILKKYK
                     "
     gene            4330..4695
                     /locus_tag="PLD67_00480"
     CDS             4330..4695
                     /locus_tag="PLD67_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOT21033.1"
                     /translation="MKSLEELRKIREEAIKNIEMRNPDKDIRVVVGMATCGISAGARP
                     VLSTFVDEVAKRNLKNVQVVQTGCIGMCTYEPIVEIYSPDKEKVTYIHVDSEKAKRIV
                     AEHLVNGRVVREYTIGSEI"
     gene            4720..6522
                     /gene="nuoF"
                     /locus_tag="PLD67_00485"
     CDS             4720..6522
                     /gene="nuoF"
                     /locus_tag="PLD67_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOT21034.1"
                     /translation="MAIFRAHVLICGGSGCTSNKSQVVRKRFEEKIKEVQLDKEVQVV
                     GTGCFGLCEEGPVVIVYPEGSFYSRMTVDKVDEIVEEHLLKGRIVEKYLYKEKVKVED
                     AKEHKSLMEVQFYSKQKRVALRNCGLINPEDINEYIARDGYMALGKVLTEMSPQDVIQ
                     LMKESNLRGRGGAGFPVGLKWGFAAPNKADQKYIICNADEGDPGAFMDRSILEGDPHA
                     VLEAMAIAGYAIGATKGFIYVRAEYPIAVRRLHIAIDQARELGLLGKNIFDSGFDFDI
                     ETRLGAGAFVCGEETALMQSIQGNRGMAKVKPPFPANKGLWGKPTVINNVETLANIPQ
                     IILKGPEWFKSFGTEKSPGTKVFALGGKITNTGLVEVPMGITLREVIYDIGGGCPGGK
                     AFKAVQTGGPSGGCLTAGHLDTPVDYENLAAVGSIMGSGGMIVMDEDNCMVDIARFFL
                     DFIVDESCGKCTPCRVGTKRMLTILERITQGKGTLEDLEELESLANSIKNSSLCGLGQ
                     TAPNPVLATLRYFRDEYIAHVVDKKCPAKNCKALLTFEIDKDKCTGCTLCARNCPVSC
                     ISGTVKNPHEINQELCIKCGNCYSVCRFGAVQKN"
     gene            6538..9990
                     /locus_tag="PLD67_00490"
     CDS             6538..9990
                     /locus_tag="PLD67_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT21035.1"
                     /translation="MALVKLNINGKELTAEAGKTVLEAALENGIEIPHLCFDERLEVY
                     AGCGLCIVEIEGQPKIARACATPVSDGLVVRSDTQKVIEARNNALNLLVSQHIGDCKA
                     PCTLNCPANTDVQGYVGLVANKQYIESLKLIKEKLPLPACIGRVCPHPCEKACRRQYV
                     EEPVSIACIKTFAADYDLNTGNPYIPELKPATGKKVAVVGAGPAGLSAAYYLLADGHE
                     VDIFEAMPKPGGMLRYGIPEYRLPKNVVDAEVAIIEKMGANFNYGVKVGEDISLEYLQ
                     NKYDAVFLGIGAWKSSPMRCEGENTPGVLGGIDFLIKVAENKPVKIGEKVIVVGGGNT
                     AMDVARTSIRLGAKEVRVVYRRSEEQMPAEEIEIKEAKEEGVIFSFLSAPSLVLSDGE
                     KVTGLKCEKMVLGEKDASGRQSPLPTGEFVEFEADTIIAAIGQEVDCGKINVGQGKKK
                     NIVINEGTFETNLKGVFAGGDAATGPKIAIEAVAQGGRAAAVISSYLAGEMIPYKNQP
                     LVYTEVTKEDYKDEERIPRVPHRTVEPEIRKHNFQPILPTMTEEEAIREGQRCLECGC
                     KDYFECQLVDYIKKFEVDTKKYHAENEKKFKETDHPFISQNVDKCVLCGLCVRACDEV
                     VGASALGFVERGNATFIAPAFEQELKVTSCISCGQCIDVCPVGAWLDRRGNMKEIPLD
                     LTQTKSVCGYCSVGCDVVYEHKDNMVYLASSPKENEIPVCGKGKFGIEHINDENRILQ
                     SKVKVKGKYEPIELPVALFETAKRLRSIQNMYGDDQVAFVVSPKLTNEEFKYIKAVAD
                     ELNTKYKGSFTLDSESGIEYVLGKNESTIRAEELDNADLIISAGQLYEHHPAAGMKLR
                     RLSNTKKIISASTIKTKLNNFAVTADVTDYEVFFTRVLKALVDNGVIKKEAISRYENG
                     EELIKALDKTEVLPEAAKVAEAFKKARKPIIVADENTVPKAALKVLADITVLAGNNNR
                     PHRGLITLKAKANSQGAWNIGFRTPGEEIRKALLNNELKGLVVIGEDILGNVPELKKA
                     ADNLKFFAAFDIMENETTSNAEYVLPLCSIAESNGTIVSADGTVRNVYQAIKPLTGTS
                     NLEMFAKLAGLLNAEYKEDAKDEAKVKLYVPTLKGKEKEYEVYDTVEKAFLAKLKENC
                     IKAV"
     gene            10370..11158
                     /locus_tag="PLD67_00495"
     CDS             10370..11158
                     /locus_tag="PLD67_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT21036.1"
                     /translation="MKKIFKSILLTASLTIGLSMFVAADTYAATYTIAKGDSLYKISK
                     AYNTTVSNIMVANDLKSYDLSIGQVINIPDVKYIVKPGDTLYKISQKYNIPLFNLRQA
                     NNIWTDYIYVGQVLEIPLPPAAVKEELKVAKQPNEGYKDYTAEELDLLARLIMAETES
                     QSYEAKVAVGSVVMNRVKSGLFAPTISGVINQNINGYYQFTPVENGWIKRAANEECIK
                     AAKEALSGVDTTNGALFYYDDSTTNQWILSKPVSVKYGRMIFAY"
     gene            complement(11198..>12357)
                     /gene="mgtE"
                     /locus_tag="PLD67_00500"
     CDS             complement(11198..>12357)
                     /gene="mgtE"
                     /locus_tag="PLD67_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HOT21037.1"
                     /translation="ISPEQKADIINAVTDKELKYILDELFFDDMIDLIEEMPANVVNK
                     ILINSTQEERTLINQFLKYPAHSAGSIMTIEYVELKKNMTVKEALEHIKKTGINKETI
                     YTCYVTDKKRKLEGIVSLRNLVVSDPDQSINDIMQEEVIYVETHDDQEIIANIFKKYG
                     FMALPVVDKEHRLIGIITFDDIMDVMEDEATEDFQRMAAMVPSDEAYLDLSVIALAKQ
                     RIPWLMILMLGATVTGGIITKFENILSTVVVLTAFIPMLMDTGGNSGNQSSTLIIRGL
                     ALGNVNQKDIWKVLWKEFRISLLVGIILASVNFAKNMLFDKVGLNVSLTVSLTVATTV
                     MLAKMVGGTLPLIAKKLKLDPAIMAGPLITTIVDALVLLVYFGVASALLIK"
BASE COUNT         4443 a         1925 c         2624 g         3365 t
ORIGIN      
        1 aagaaaaaaa taattatggg acacgcttca atatggggtg actctctgag ggatagatag
       61 aggaaggggg acacaccctt aaggagggta agtctctgag gtcagacctt aagggaatgt
      121 ccccgtactt ggaatgtccc gtacttgggg ggattaacat ggatacctat caaattatat
      181 ccattatagg cgttataatt tttatatatt tcatattttt taaaaatcga cgctctgcac
      241 aaaaagaaat aagcagctta aatgaaattg aatatatagg ctcatcaact atgaaatttg
      301 atgatgtggc cggaaactat gaagcaaaac aaagtcttct tgaactggta gacttcatac
      361 aaaatcctga aaaatacagc aagtttaatg caaggatgcc aaaaggggta cttctttacg
      421 gcgaaccggg aacagggaag actttaatgg caaaggcctt ggcaggtgaa gccggggtac
      481 ctttctatgc agtatcgggt tctgattttg tgcaagttta tgcaggtctt ggagccggaa
      541 gaataagaaa tttattcaaa aaagctaaaa aatcaggcaa aagtgttatt tttatagatg
      601 aaatagatgc cataggaaag aaaagaatgt caggtagcat taaaggaagc gatgaaggtg
      661 acaggacctt aaatgcactt ctaaccgaga tgtcgggttt tgccgatgat tcaggaactg
      721 tagtaattgc agccacaaac agaattgata ctttggacag tgcattgtta agaccgggaa
      781 gatttgacag acaaattgaa ataatgcttc cggacaaaaa tgcaagaaaa gatattataa
      841 agctttattt ggacaaaaaa aatgcagaag atattaatat agacgagctg gctgatttga
      901 ctgtttattt cagcggagca atgattgaaa atttaataaa tgaagcagca cttttggcaa
      961 taagacaaaa ttcagataaa attaaggaag aacatatcaa aaaagcgtat ttgcaaatat
     1021 tggcgggagt tgaaaaagag gattatgata atggatttca aaaaaccatt acatcatatc
     1081 acgaagcagg acatgcattt gtttcaagac atttgctgcc tgaaaataaa attattcgag
     1141 tttctgtaat tcccaccagt aagggagccg gaggatacac gctgagcact cctgactcgg
     1201 aagaggtaat gaccaaaggg aaaatgataa atcatttatc tgttctaatg gcggggagag
     1261 catctgaaga agtattcttc gggaatgaaa atattaccgc cggagcacaa aatgacattg
     1321 aaaaagtaac ctctttggca gtggattatg tttcaaaata cggcatgaat gatgcaacat
     1381 tcataaatta tcagctgctg tccgataaac tgagaatacc attgacatca gttgaagtct
     1441 cggtaaaaaa attgacttca gaaatatatg aagaggtgat taggttaatt gaatgcaata
     1501 aggaaaaagt tgagaaaata gcaagactgt taatggaaaa agaagttatt taccaggatg
     1561 aattggataa tttgatttaa aaatatagtt gaatattatc taaaacacag gttaaaatac
     1621 tagtgaataa agcagccttc ataaaaaccg gttgtaatta ttctaaaaat ttgatataat
     1681 taaattaaag atagcctgcg gtgttcggaa atcttttaat tcaaccgaca tattgtaaaa
     1741 gggaattcgc gtttgactgc ctatgacaag cccgctttga cgggacgcca gaatcagcca
     1801 acagaaatcc cacctgccta ggtgcgggtg tgaattatag gtttgcggca ttgcgggtat
     1861 tattatgtta tgaataaata tagatttgtc atactttaat ttgtcattct gagaggggca
     1921 cgtagaatct cactgcatac tgagattttt actgccgctc aggatgacat ttttttagga
     1981 aaacgggaaa ataacatttg cctttatcat gaaattcata tataatggta acaaaatagg
     2041 aatacattac caatgattat aggaggaata tatgataacg atcaaagacg tcgccaggct
     2101 tgcagaggta tcgatttcaa ccgtatcaag agtgataaac gactcaaagc ctgttagtcc
     2161 cgaagttagg aagagggttt taaaagttat tgaagaaacc ggatataagc ccaatgatgt
     2221 tgcgagaagc cttgtaacaa gaagatcata tttaataggt gttatagtga ataatttagc
     2281 caacagctac gttgcagaca ctgtgagagg aattgaagaa ataggaaaaa tgtacggata
     2341 tgatattctt ctttgcagca gctatggcag caaggaaact caggaaaaat atctgcagct
     2401 gctagacaga aagcaagccg aaggtttgtt ccttgtagga agtatatttg atgaagaaat
     2461 tattaaattg gctaaaagct ttaataaacc ctgcgtattt tttacaagag atattcgcga
     2521 aaacatgaat tatataacaa ttgactgcaa atctgcaatt tatgaagtaa caaaatattt
     2581 attcgaagaa ggccacagaa aaattgcata tttttccgac tatgaaaaca gaactaccgt
     2641 agaagacgaa aaggttgaag gatacttaaa agcaattgaa gataatgaaa tggaatattc
     2701 taaaatatat gttgtcggag gaagcaagca caatgaagca tatgatttag ggaaatcaat
     2761 aattgatgaa gcaaaggatt ttactgcatg tgtttgcagc tctgatgaaa tagcaatagg
     2821 gatattggac agctttttag acaacggaat taaagttccg gacgatgtgt ctattgtagg
     2881 atttggaaat attagggagg gaaaattcgt gagacctgaa ctgactacaa taggtgagcc
     2941 ttattatgat gttggagcgg taggtatgag aatgcttata aaaatgataa aaggcgataa
     3001 aattcaacaa ggacgtatgg agctgccatt taccatagaa aaaagaaaaa gtgtaaaaaa
     3061 tttgaataaa taatacaaag tttgttgaaa ttaaacgagt aatttaattt tgagcgcgac
     3121 aattattgat aaaataatca aagtaatacc cctataatga aaagacatct ttattgcagg
     3181 gggattacaa atgaaaaaga gatttatttt cacgatgatc gtgttgctgc ttttcagtta
     3241 caattcaatt aacggctatg aatatcataa agaaatagag actattgagt atttgctaaa
     3301 tgagcggata acaataatga atgaatttct ctacggatat aaagatatgc aaagcttgga
     3361 ggagaagtta agcaaaattg aatccgataa tttactgcaa aatgacttgg atattttata
     3421 taaggtaaag aacagcccta cagattatga attggcaatg aaggttaagg ttaatgaaat
     3481 ttatgatata gagcttaccg atgacggata tttggtaaat gctgacctga attggcttat
     3541 gagaggatat gacggtgaat ttaatttgat tagaaattat caaattaaat gcatagaaga
     3601 tggcgggcaa tcatatttgg caaaattaac ggtattaaac aatacagcag aataatttaa
     3661 tatttcaaaa ataatgaata agtattttaa tacaaaagaa tcctcataat gaggattctt
     3721 ttgcttataa attatcaaaa ccttaaataa aaatcaacaa tattttgact ttttatgttg
     3781 ataattgtat aattaatgtt atagttattt ttaaaataaa ttggaggatg gcaaattgca
     3841 aaatgagttt ttacaatcaa aaaaaagtga atttgccgaa ttgaaagagt atatcgattc
     3901 ggtaaaaaac tcacaaggcg ttaccatgca aatattgcag aaagcacaaa gtttatttgg
     3961 atacttgcct cttgaagttc aacagtttat atcagatgaa acaaatatac cactggccga
     4021 tatttatgga gttgtaacct tttacacaca gttttccacg gaagaaaagg gtaaacacaa
     4081 aattggatta tgtcttggta cagcttgtta cgtaaagggc tcgcagggca ttttagacaa
     4141 gatttctgat gaattaaatg ttaaagtcgg gaaaacaacg gacgataagc tgtttacctt
     4201 agaagctacc cgatgcttgg gctgctgcgg attggccccg gttatgatgg ttgacgatga
     4261 tgtttacggc aaattggata caacaaaaat ccccggtatt ctaaaaaaat ataaatagga
     4321 gggagcagca tgaagtcgtt ggaagaatta agaaaaataa gagaagaagc aataaaaaat
     4381 attgaaatgc gaaatcccga taaagatata agagtggttg taggtatggc cacatgcgga
     4441 atttcagctg gtgccagacc tgttctttct acattcgtag acgaagtagc aaaaagaaat
     4501 ttaaaaaatg tacaagttgt tcaaacaggc tgtataggca tgtgtacgta tgagccgatt
     4561 gtagaaatat attcccctga taaggaaaag gtgacatata ttcatgttga ctctgaaaaa
     4621 gcaaaaagaa tagttgcaga gcacttggtt aatggaagag ttgtaagaga atatacaata
     4681 ggttcggaaa tttagattaa tgaaaatgga ggtaaaacca tggcaatttt cagagctcat
     4741 gttttaatat gcgggggaag cggatgtact tctaataaat ctcaagtagt cagaaaaaga
     4801 tttgaagaga aaataaaaga agtacaatta gacaaagaag tccaggttgt gggaacaggc
     4861 tgctttggtc tttgcgaaga aggtcccgta gtcatagttt atccggaagg ctcattttat
     4921 tcaagaatga ctgtggacaa ggttgacgaa atagtagaag agcacttgtt aaagggaagg
     4981 atagtcgaaa aatatttata taaagaaaag gtaaaggtag aagatgccaa agagcataaa
     5041 tccttaatgg aagttcagtt ttacagcaag caaaaaagag ttgcactcag gaactgcggt
     5101 ttaattaatc ctgaggatat taatgaatat atagcaagag acggctatat ggcattagga
     5161 aaagtattga ctgaaatgtc cccccaagat gtaatacaat taatgaaaga atccaacctt
     5221 cgaggaagag ggggggcagg cttccctgta ggattaaaat ggggctttgc agcaccaaat
     5281 aaagccgacc aaaaatatat aatatgcaat gctgacgaag gagatcccgg tgcatttatg
     5341 gacagaagta ttttagaggg agaccctcat gcagtattgg aagccatggc tatagcaggt
     5401 tatgcaatcg gagctacaaa gggatttatt tatgtcaggg cagaatatcc catagctgtt
     5461 cgcagactgc atatagcaat cgaccaagca agagagcttg gattattagg taaaaatata
     5521 tttgattcag gatttgattt tgatatagaa acaagacttg gagccggtgc atttgtatgc
     5581 ggagaagaaa cagcactgat gcagtcaatt caaggaaaca ggggtatggc gaaggtaaag
     5641 cctccattcc ctgctaacaa aggcttatgg ggaaaaccga cggtaataaa taatgttgaa
     5701 accttagcta acataccgca gattatatta aaaggacctg aatggtttaa aagctttggt
     5761 acggaaaaat cacccggaac aaaagtattt gcattgggcg gaaaaattac taataccgga
     5821 ctggttgaag taccgatggg aataacatta agagaagtaa tatatgacat cggaggagga
     5881 tgccccggcg ggaaagcatt taaagcagtt caaacaggag gaccttccgg tggttgcttg
     5941 acagccggcc atcttgatac tcccgttgac tatgaaaatc ttgcagctgt aggctcaata
     6001 atgggctcag gcggaatgat agtaatggat gaagataact gtatggttga cattgcaaga
     6061 ttcttcctgg atttcattgt tgatgaatca tgcggcaaat gtacaccttg cagagtggga
     6121 acaaaaagaa tgctcaccat acttgaaaga attactcaag gtaagggaac cttggaagat
     6181 ttagaagaac tcgaaagctt ggcaaacagt ataaagaatt catcgctctg cggtcttgga
     6241 caaacagctc caaaccctgt attggcaaca ctcagatatt ttagagatga atatattgca
     6301 cacgtggtgg ataagaaatg tcccgctaaa aattgcaaag ctttgttaac ttttgaaatc
     6361 gacaaggata aatgtacggg atgcactctt tgtgcaagaa attgtcctgt aagttgtatt
     6421 tcaggaactg ttaagaatcc tcatgaaata aatcaagaat tatgtattaa atgcggaaat
     6481 tgctacagcg tatgcagatt tggcgctgta caaaaaaatt aaaagggagg attagccgtg
     6541 gccttagtaa aacttaatat aaatggaaaa gaacttacag ctgaagccgg gaaaacagta
     6601 ctggaagcag ctttagaaaa tggcatagaa attccgcact tgtgttttga tgaaagattg
     6661 gaagtgtatg caggatgcgg actatgcatc gtggaaatcg aaggacagcc aaaaattgca
     6721 agagcttgtg caactcccgt ttctgacgga ttagttgtga gatcagacac tcaaaaagta
     6781 atagaagcaa ggaataatgc gcttaattta ttggtttcac agcatatagg agactgtaag
     6841 gcaccttgta cgctaaactg tccggcaaat actgatgtac agggatatgt cggtttagtt
     6901 gcaaataaac aatatattga atcattaaaa ctgataaaag aaaaattacc tttgcctgca
     6961 tgcataggaa gagtatgccc tcatccttgt gaaaaagcat gccgaagaca atatgtggaa
     7021 gagccggtat caatagcgtg tattaaaaca tttgcagcgg attacgactt gaatacaggc
     7081 aatccctata ttcctgaatt aaagcctgct acaggcaaga aggttgctgt tgtgggagca
     7141 ggtcctgcag gattgtcagc agcatactat ctgttggcgg acggacatga agtagatata
     7201 tttgaagcaa tgccaaaacc cggcggaatg cttagatacg gtattcccga atacaggctt
     7261 ccgaaaaatg tagttgatgc agaagttgca attattgaaa aaatgggtgc aaatttcaac
     7321 tacggcgtaa aagtcggcga agacatttct ttggaatatt tgcaaaataa atacgatgca
     7381 gtgttccttg gcataggagc atggaaaagt tcacctatga gatgtgaggg ggaaaatact
     7441 ccgggagtat taggcggaat tgatttctta ataaaagtag cagaaaacaa gccggtaaaa
     7501 ataggcgaaa aagtaatagt ggtaggcggc ggcaacactg ccatggacgt tgcaagaacc
     7561 agcatacgtt taggagcaaa agaagtaaga gtagtttaca gaagaagtga agaacaaatg
     7621 cccgccgaag aaatagaaat taaagaagcg aaagaagaag gagtaatatt ctcattcttg
     7681 tcagctccgt cactggtttt aagcgacggt gaaaaagtaa ccggacttaa atgcgaaaag
     7741 atggtccttg gtgaaaagga tgcatccgga agacaaagtc cccttccaac cggcgaattt
     7801 gtagaattcg aagcagatac gattatagcg gcaataggtc aggaagttga ctgcggtaaa
     7861 attaatgtag gacagggaaa aaagaaaaat atagttataa atgaaggcac ttttgaaaca
     7921 aatttaaaag gtgtgtttgc aggaggagat gcagcaacag gacctaagat tgctatagaa
     7981 gcagttgcac aaggcggacg tgcggcagca gttatttcaa gttatctggc aggtgaaatg
     8041 attccttaca aaaaccagcc attagtatat actgaagtaa cgaaagaaga ttataaagac
     8101 gaagaaagaa ttccaagagt tcctcacaga actgtagagc cggaaataag aaagcataac
     8161 ttccagccca tacttccaac aatgacagag gaagaagcaa taagagaagg acaaagatgt
     8221 cttgaatgcg gttgtaagga ttactttgaa tgccagttgg ttgactatat caagaagttt
     8281 gaagttgata ccaaaaaata tcatgcagaa aatgaaaaga aattcaaaga aacagaccat
     8341 cccttcattt cgcaaaatgt tgataaatgt gtactttgcg gattatgtgt gcgtgcatgc
     8401 gacgaagttg tgggagcatc agcattagga tttgtggaaa gaggaaatgc aacatttata
     8461 gcacctgcat tcgaacagga attaaaagta acaagctgta tttcatgcgg ccagtgtatt
     8521 gatgtgtgtc ctgtaggagc ttggcttgac agaagaggca atatgaagga aattcctctg
     8581 gacttaacac aaaccaagtc agtgtgcggc tattgctcag tagggtgcga tgttgtatat
     8641 gagcacaaag acaatatggt ttacttggca tcaagtccta aagaaaatga aatacccgta
     8701 tgcggtaagg gtaaattcgg tatcgagcat attaacgatg aaaacagaat cctccagtca
     8761 aaggttaagg taaaaggaaa gtatgagcca atagaattac ctgttgcttt atttgaaacg
     8821 gctaaacgct taagaagcat acagaatatg tacggagatg accaagtagc atttgtagta
     8881 tcaccaaaat taacaaacga agaatttaaa tatattaaag ctgtggcaga tgaattaaat
     8941 actaaataca agggttcatt tacacttgat tcggaatccg gtattgaata tgtattaggc
     9001 aagaatgaat ctaccataag agcagaagaa ttggacaatg cagatttgat tatttctgca
     9061 ggtcagttgt acgagcatca ccctgcagca ggaatgaaat taagaagact ttctaacact
     9121 aagaaaataa tttctgcatc aactattaaa actaagctta ataactttgc tgttacagca
     9181 gacgtaacag attatgaagt gttctttaca agagtactga aagcattggt tgacaacgga
     9241 gttattaaga aagaagcaat aagccgctat gaaaacggag aagagctgat taaggcattg
     9301 gacaaaaccg aagtacttcc tgaagcagct aaggttgcag aagctttcaa gaaggccaga
     9361 aaacccatta tcgttgcgga tgaaaacaca gttccaaagg cagcactgaa agtcctggct
     9421 gatataaccg tattagcagg aaataataac cgtcctcaca gaggcttgat aactcttaaa
     9481 gctaaagcaa acagccaggg agcttggaat attggtttca ggacaccggg agaagaaatc
     9541 aggaaagcat tgttaaacaa tgagcttaaa ggcttagttg taatcggcga agatatattg
     9601 ggaaatgttc ctgaacttaa gaaagcagca gacaatctaa aattctttgc agcctttgac
     9661 ataatggaaa atgaaactac ctctaatgcc gaatatgtac ttccgctttg cagcatagct
     9721 gaaagcaatg gtaccatagt aagcgcagac gggaccgtaa gaaatgtata tcaagcaata
     9781 aaacctttaa caggcacgtc aaaccttgaa atgttcgcaa aattagccgg actgttaaat
     9841 gcagaataca aagaggatgc taaggacgaa gcaaaagtta agctttatgt acctacgctg
     9901 aaaggcaagg aaaaagaata cgaagtatac gacacggtag aaaaagcatt ccttgcaaag
     9961 ctaaaagaaa actgcataaa agcagtttaa tgacaagggg acgggggtac tgtcacaaac
    10021 ccaccgggga cggttctcgc tggacaaaaa ttttattaaa ttagagattc tgaaaattca
    10081 gactctcttt ttttacccac tggggacggt tctcgctgga caaattcctc ttaagaaaac
    10141 attaatattt agttgttaaa aaatgtttat ataaattagc cttgcaattc caacgttatt
    10201 ttaattatct acccctttgg cttaaagaat ttatttcttt gcatgtgtta tatatcctcg
    10261 attgcaaggt ctttccattg attccatata agatattgac tacaattttt acaaccgtat
    10321 ttcttaaaga gtacctgggc aggattctgg aagacataag gaggataaaa tgaaaaaaat
    10381 ttttaagtca atattattaa cagcaagtct gactatcgga ctatccatgt tcgtagctgc
    10441 tgatacatat gcagcaacct acacaatagc taaaggtgat tctttgtaca aaataagcaa
    10501 agcttacaac acaaccgtat caaatataat ggttgcaaac gatttaaaaa gctatgattt
    10561 aagtatcggt caagtaatta atattccgga cgtaaaatac atagtgaagc cgggtgatac
    10621 attatataaa atatcacaaa aatataatat tccactgttc aatttaagac aagccaacaa
    10681 catttggact gattacatat atgttggaca ggttttggag attccgttgc caccggcagc
    10741 agtaaaagaa gaacttaagg ttgcaaaaca gccgaacgaa ggatataaag actatactgc
    10801 cgaagaattg gacttgcttg caagattaat aatggcggaa acagagagcc agtcttatga
    10861 agctaaggtt gcagtcggct ctgttgttat gaacagggta aaaagcggtt tgtttgcacc
    10921 gacaataagc ggtgtaatca accaaaacat caatggatat taccaattta cccctgttga
    10981 aaacggttgg attaaaagag ctgccaatga agaatgtatc aaagctgcaa aagaagcact
    11041 aagcggagtc gatactacaa acggtgcatt gttctactat gacgattcaa caacaaatca
    11101 atggatttta tcgaagccgg tttctgttaa atacggaaga atgatatttg catattaata
    11161 aattaaaaac agaggaagat taatttcctt tgctttttta ttttatcaga agagcagatg
    11221 caactccaaa ataaaccaag agtaccaatg catctacaat tgttgtaatt aatggtcctg
    11281 ccattatggc agggtctaat ttaagctttt ttgctataag cggaagggtc ccgccaacca
    11341 ttttagctaa cataactgta gttgccacag ttaaggatac agttaaagaa acatttaaac
    11401 ctaccttgtc aaacagcata ttttttgcaa aatttactga tgctaatata attccaacca
    11461 ataagcttat tctgaattcc ttccataaaa ccttccaaat gtctttttgg ttcacatttc
    11521 ctaatgctaa cccacgaatt attaatgttg aagattggtt tcctgagttt cctccggtat
    11581 ccataagcat tggaataaat gcggtaagaa caacaacagt gctgaggata ttttcaaatt
    11641 ttgtaataat gccgcctgta acggtagccc caagcattaa tatcataagc catggaatcc
    11701 tttgttttgc tagtgctatt acacttaaat ccaaatacgc ttcatccgat ggtaccatgg
    11761 ctgccattct ttggaagtct tctgtagctt catcttccat tacatccatt atatcatcaa
    11821 acgtaataat acctattaat ctgtgctcct tatcaacaac aggcaatgcc ataaaaccgt
    11881 attttttaaa aatatttgct attatttcct gatcatcgtg agtttctaca taaataactt
    11941 cttcttgcat aatatcgtta atgctttgat caggatcaga aactaccaga tttcttaatg
    12001 aaactattcc ttctaatttt cttttcttgt ccgtaacata acatgtatat attgtttcct
    12061 tgtttatccc tgttttttta atatgttcaa gagcttcctt aactgtcatg tttttcttaa
    12121 gctcaacata ttcaattgtc attatgcttc ctgccgaatg agcaggatat tttaaaaatt
    12181 gattaattaa agttctttct tcctgagttg aattgattaa aattttattg acaacattag
    12241 caggcatttc ttcaattaag tctatcatgt catcaaaaaa cagctcatcc aaaatatatt
    12301 ttaattcttt gtctgtcacc gcattaataa tatctgcttt ttgttccggt gaaatat
//
LOCUS       DAOSOW010000007        10866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_6029_length_10866_cov_4.403848, whole genome shotgun sequence.
ACCESSION   DAOSOW010000007 DAOSOW010000000
VERSION     DAOSOW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 10866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10866
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6029_length_10866_cov_4.403848"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..288
                     /gene="rpmH"
                     /locus_tag="PLD67_00505"
     CDS             154..288
                     /gene="rpmH"
                     /locus_tag="PLD67_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOT21038.1"
                     /translation="MKRTYQPKRKQRSSEHGFLKRMSTKSGRNVIKRRRARGRKKLSA
                     "
     gene            338..673
                     /gene="rnpA"
                     /locus_tag="PLD67_00510"
     CDS             338..673
                     /gene="rnpA"
                     /locus_tag="PLD67_00510"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOT21039.1"
                     /translation="MIIIKKNNDYKAVYNCNNSISDNNLVLFIKKNNYNYNRFGFTTA
                     KKIKKAVHRNVIRRRLKEIVRANEHYLEEGYDFVLMARINAVDSDYKSLESSFIKLMK
                     RKKIYRYTI"
     gene            670..879
                     /gene="yidD"
                     /locus_tag="PLD67_00515"
     CDS             670..879
                     /gene="yidD"
                     /locus_tag="PLD67_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOT21040.1"
                     /translation="MSKVLIFIIRIYQKFSSKSAVRHCRFYPTCSQYFVEALQKYGFL
                     KGSYLGIRRILKCHPFNPGGYDPLK"
     gene            890..1768
                     /locus_tag="PLD67_00520"
     CDS             890..1768
                     /locus_tag="PLD67_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOT21041.1"
                     /translation="MNLDFIAVPMGMLVKLVYNMVSVVDSKYLSAYSIAIIISTILFK
                     LIVLPLTLKQSKSMKKMQELSPKIQELQQKYGKDPQTLQRKQMELYKEANYSPFSGCL
                     PLLIQFPILIAFFYVIREPVKYVFGDQAFYDSINRAFLWIKDLNVNENTVWFLKNNDW
                     IVLTNAIKEEFSISSSMHLNQVMEVLNAQDIMVNGLSMGGMSLPFLGSALPVLAVITG
                     ITTYLTTKMTAQPSINEQQASTQKTMNMMMPIMIFVFSIQFPAGLALYWVISNLFQLV
                     QQFIVLNSSKNPKEEL"
     gene            1772..2395
                     /gene="jag"
                     /locus_tag="PLD67_00525"
     CDS             1772..2395
                     /gene="jag"
                     /locus_tag="PLD67_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HOT21042.1"
                     /translation="MRNVTIEKPTVEEAIAAALKELRAENNEVEIEIINEPSKGFFGL
                     GSKDAKVKVTITNGPEERARKFIDILLKKMSIEADYEVNYSDNILNVDIVKIGEDDKG
                     IVIGKRGKNLDEIQFLLNLMVNKGRENYVRVIFNVEDYRAKREETLKRLALKMADKCR
                     YYNRKVKLEPMNPYERRIIHSTLQDVKGIITYSEGEEPYRKVVIDQK"
     gene            2543..3916
                     /gene="mnmE"
                     /locus_tag="PLD67_00530"
     CDS             2543..3916
                     /gene="mnmE"
                     /locus_tag="PLD67_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOT21043.1"
                     /translation="MFSDTIAAIATFPGNAGINIIRLSGNDALDIAYKIFVDKNKQKP
                     DNLKPRYLHYGFIVDKDKKVIDEVLLSYMKAPNTYTREDIIEINCHGGIISAKKILDT
                     VLHYGCRTAERGEFTKRAFLNGRIDLTQAEAVIDIINSKTESSHEISIKHLEGRLSKQ
                     INAVIDEIMELLANIEVNIDFPEYDEDEITIEKVKALCHNLVPELDKLINTADTGKII
                     KEGIKTVILGKPNVGKSSLMNFLLNENRAIVTEVPGTTRDTIEEYVNIKGVPLRIIDT
                     AGIRETDDMVERIGVEKALEKVKEADLVIMIFDSSKELEKEDESILDYIKDKKVIYIR
                     NKTDLESRLDLSSYKKIEEEIINVSILQNQGLDDIINKIYSLFFKGRLDIRDELIINN
                     VRHKNLLIKAKNNIEEVLKSIENNMPIDFIEIDLMQAMENLGLIVGKSVSDDLIDKIF
                     DEFCIGK"
     gene            3930..5819
                     /gene="mnmG"
                     /locus_tag="PLD67_00535"
     CDS             3930..5819
                     /gene="mnmG"
                     /locus_tag="PLD67_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOT21044.1"
                     /translation="MEDRVREFLEESIDVLVIGAGHAGCEASLATARLGLKTIMLTLS
                     LDSIAMMPCNPNIGGTAKGHLVREIDALGGEMALNIDKTMIQCKMLNRSKGPAVHSLR
                     AQADKKKYHREMKKVIENQKNLRVIQTEATKIKVNEDKSFNVYTKLGAYYRARAVIVT
                     TGTYLKGRIYMGELNYSSGPDGLAPANELSDSLMDLGIELIKFKTGTPARIHKDSINY
                     EKMTIQTGDDEIIPFSFMNDEINIEQVPCYITYTTPQTHELIRNNLDRSPLYGGVIKS
                     VGPRYCPSLEDKVVKFSDKEKHQLFVEPEGLDTKEMYIQGMSTSLPYEVQIQMYRTMI
                     GLENAEIMRPAYAIEYDAIDPTQLKPSLELISIKNLFFAGQINGSSGYEEAAAQGLIA
                     GINAYLSLNNKEPFILDRSEAYIGVLIDDLVTKGTTEPYRMMTSRAEYRLLLRQDNAD
                     ERLTEKGYKIGLVTEERYKRFKDKRQRIENEINRLKNLKITPKADTNKALEDMGSAAL
                     KIPTTLYDLLRRTELNYDNTLFLDPKRPVLRKEIKDYVETVIKYEGYIQKQIAQVEQF
                     KKLENKKIPEDINYDDVKSLRIEARQKLNKIRPNSIGQASRISGVSPADINVLLIYLM
                     TYNKE"
     gene            5830..6543
                     /gene="rsmG"
                     /locus_tag="PLD67_00540"
     CDS             5830..6543
                     /gene="rsmG"
                     /locus_tag="PLD67_00540"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOT21045.1"
                     /translation="MIKTLEEGFKELKLPYSKDIENNFIIYRDLLKEWNKKINITSIE
                     NDGEIYIKHFIDSVLLLNENNINENKTIIDVGTGGGFPGLPLKIVNNNYKITLLDSLR
                     KRIDFLSEVIKALNLNDVKLIHGRAEDYGQNKEYRECYDICVSRAVAPLNVLSEYCIP
                     FVKVGGYFAAYKSDNISQEISSSDNAIKKLGGEIKEIKKINLPGTDIIRKIVIIEKTD
                     KTEIEYPRKAGKPAKDPLK"
     assembly_gap    6631..6730
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6731..7171
                     /locus_tag="PLD67_00545"
     CDS             <6731..7171
                     /locus_tag="PLD67_00545"
                     /inference="COORDINATES: protein motif:HMM:NF036800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HOT21046.1"
                     /translation="ISQTQLAQKLGKSQSAIANKVRILKLPESVKEKLREGNLTERHG
                     RALLKINDEEILLNIVEKIIKKDLNVSETEKIVDSIAEELNIKKKADKRYVRNFINYK
                     IYINTIKNAYNEIVKTGIDAKFEQNESDDYIEIKVKIPKKACRG"
     gene            7176..7937
                     /locus_tag="PLD67_00550"
     CDS             7176..7937
                     /locus_tag="PLD67_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT21047.1"
                     /translation="MTKVISIFNQKGGVGKTTTNVNLCTALALKGKKVLSIDIDPQGN
                     TTSGFGFNKNKLEYTIYNVLIEDYDINKIIFKTEIENLDITPANIQLAGAEIELTNTK
                     FREKTLREKINLIENKYDFIMVDCPPSLGLLSLNALTASNTVLIPIQCEYYSLEGVGQ
                     LIDTVKLVKKNLNPKLEIEGVILNMFDGRTNLSIQVVEEVKKYFKDKVYRTVIPRSIR
                     LAEAPSFGQPIMLYDDKSKGSIAYIKLSEEFIKNN"
     gene            7952..8797
                     /locus_tag="PLD67_00555"
     CDS             7952..8797
                     /locus_tag="PLD67_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOT21048.1"
                     /translation="MSAKRKALGKGLSALIPEDLEKEDYEKIHEIDIDLISPNPNQPR
                     KLFEQDKLDELTESIKKYGVIQPIILRKDGELYTIIAGERRWRACKNANIKTIPSIVR
                     DIENKNASEIALIENIQREDLNPIDEANAYQFIMDRYGITQEQVSNIVGKSRVYVTNI
                     MRLNNLDEYVKDKIIKNLITAGHGRAILSLDKEKQKSLTDIIIRDNLSVRDVEKLVRS
                     SKKPQRREKPAKDKYIVYLEELLTERFSTRVNIKNKKDKGKIEIEYTSSDDLNRILDL
                     LKIED"
     gene            9182..9667
                     /gene="nuoE"
                     /locus_tag="PLD67_00560"
     CDS             9182..9667
                     /gene="nuoE"
                     /locus_tag="PLD67_00560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOT21049.1"
                     /translation="MTKALDVKANEKLFIELKEYIDSVKDVDGSLMQVLHKAQDIFGY
                     LPIEVQKFISSELDVPLAEVYGVATFYTQFAIEPKGKHKIGVCLGTACYVKGAQLIMD
                     KLTKELNVKVGGTTQDNLFTFEATRCLGCCGLAPVMMIDQDVYGKLEPKKIPEILAKY
                     K"
     gene            9677..10042
                     /locus_tag="PLD67_00565"
     CDS             9677..10042
                     /locus_tag="PLD67_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOT21050.1"
                     /translation="MKSLEELKKLRDESIKNIEMRNTDKDVRVVVGMATCGISAGARP
                     VLATFVEEVAKRNLNNVSVVQTGCIGMCTYEPIVEIYAPGKEKVTYIHVDSEKAKRIV
                     SEHLANNRVVVEYTIGSEN"
     gene            10064..>10866
                     /locus_tag="PLD67_00570"
     CDS             10064..>10866
                     /locus_tag="PLD67_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008824688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOT21051.1"
                     /translation="MKIFRSHVLVCGGTGCSSSNSAQIRQRFIDKIKEVNLDNEVQVI
                     GTGCFGLCEVGPVVIIYPEGSFYARMTVDRVDEIVEEHLLKGRIVKKYLYDESIKDEK
                     LKALNEVDFYSKQKRVALRNCGLINPENINEYIARDGYMALGKVLTEMTPDEVIKLVK
                     DSGLKGRGGAGFSTGMKWSFAAPNKADQKYIICNADEGDPGAFMDRSVLEGDPHAVLE
                     AMAIAGYAIGATKGYIYVRAEYPIAVRRLKIAIEQARELGLLGKNIFNTG"
BASE COUNT         4180 a         1450 c         2138 g         2998 t
ORIGIN      
        1 ctactctctg cgggaatgaa gaaatgtcct cgagcaagag tctttgcagt caggagaatg
       61 tccataattt cagtgttgac acaagtattt aatgataata taatctactg atatgataat
      121 ttatgtatag attggtaaca aggaggtgtt tcaatgaaaa gaacttatca gccgaagaga
      181 aaacaaagaa gcagtgagca tggattttta aagagaatga gtacaaaatc cggaaggaat
      241 gtaataaaga gaagaagggc aaggggtaga aaaaaactgt cagcataagg ccgcatatgt
      301 ggccttttat tccatgtaat cgcaataagg taacgaaatg ataataatta agaagaataa
      361 tgattacaaa gctgtatata attgcaataa ttcaatatct gataataatc tcgtattatt
      421 tataaaaaaa aataattaca actataacag attcggcttt actacagcaa aaaaaattaa
      481 aaaagcagta catcgaaatg ttataagaag aagactaaaa gaaatagtaa gagcaaacga
      541 gcattattta gaagaaggct atgattttgt attaatggca aggattaatg ccgttgactc
      601 cgattataaa agccttgaaa gctcattcat taaactgatg aaaagaaaaa agatatatag
      661 gtacacaatt tgagtaaagt attaattttt attatacgaa tatatcagaa gttttcttca
      721 aagtctgcag taagacattg caggttttat cccacttgct cgcaatattt tgttgaagca
      781 ctacaaaaat acggtttttt aaaaggttca tatttaggta tacgaaggat attaaaatgt
      841 catccgttta accccggagg atatgatcct ttaaaatagg agggtaaaaa tgaatttaga
      901 ttttatagca gtgcctatgg gcatgcttgt taaacttgta tataatatgg tttctgttgt
      961 tgactctaag tatttatctg cttattccat agcaattata atttccacca tattatttaa
     1021 attaatagta ttgcctttaa cattaaagca aagtaaatct atgaaaaaaa tgcaggaact
     1081 aagtccaaaa attcaggagt tacagcaaaa gtacggaaag gatccgcaaa ctttacagag
     1141 aaaacaaatg gaattatata aagaagcaaa ttacagtccg ttttccggat gtttgccttt
     1201 attaattcaa ttcccaatac ttattgcatt tttttatgta attcgagaac cggtaaaata
     1261 cgtttttgga gatcaagcat tttatgattc catcaacagg gcttttttat ggattaaaga
     1321 tttaaatgtt aatgaaaata cagtatggtt tttaaagaat aacgattgga ttgtattgac
     1381 aaatgctata aaagaagagt ttagtataag ttcaagcatg catttaaatc aagttatgga
     1441 agtattaaat gctcaagata tcatggttaa cggattgtca atgggcggca tgtctcttcc
     1501 ttttttggga tctgcccttc cggtattggc tgtaattaca ggtattacaa cataccttac
     1561 cacaaaaatg acggcgcaac cctccataaa tgaacagcaa gcctcaactc aaaaaaccat
     1621 gaatatgatg atgcctataa tgatatttgt attttccata caatttcctg caggattggc
     1681 attatattgg gttatatcaa atctattcca gttagtgcag caatttattg ttttaaattc
     1741 gtccaaaaat ccgaaggagg aattataata gatgagaaat gtaacaattg agaaacctac
     1801 cgttgaagaa gctatagccg cagcacttaa agaactccgc gccgagaata atgaagttga
     1861 aattgaaata atcaatgagc cttcaaaagg ttttttcggt ttaggcagca aggatgcaaa
     1921 ggtaaaagtt actattacca atggtcctga agagagagca agaaaattta tagacatatt
     1981 gcttaaaaaa atgagcatag aagctgatta tgaggttaat tattctgaca atattttaaa
     2041 tgttgacatt gtaaaaatcg gagaagacga taaaggaata gttatcggca aaagaggtaa
     2101 aaatttagat gaaattcagt ttctcttgaa tctaatggta aataagggaa gagaaaacta
     2161 tgtgcgagta attttcaatg ttgaagatta cagagcaaag agggaagaaa ctttaaaaag
     2221 attagctctt aaaatggcag acaaatgcag atattacaat cgcaaggtaa aacttgagcc
     2281 tatgaatcct tatgaaagaa gaataataca ttcaacatta caggacgtaa aaggtattat
     2341 tacttattcc gaaggagaag agccatacag gaaagtagta atagaccaaa aataacaaaa
     2401 ttccaaccac ttataaccct gaaaaagggt tattttatat tttcttggtt acagtaattc
     2461 aagatagaaa aatatgtaca tttatataaa attaaggtaa aatattataa ggtatgaaga
     2521 cacggaaagg taagatgaag atatgtttag cgatactatt gctgcaattg caacatttcc
     2581 cggcaatgca ggaattaata taataagatt aagcggaaat gatgcattgg atattgcata
     2641 taaaattttc gttgataaaa ataaacagaa accggataat ttaaagccaa gataccttca
     2701 ttacgggttt attgtggata aagataaaaa ggtaatagat gaagtactct tatcttacat
     2761 gaaggctcct aacacatata ccagggaaga tattattgaa atcaactgtc atggggggat
     2821 tatttccgca aaaaaaatac tggatactgt ccttcattat ggctgcagaa cagctgaaag
     2881 aggagaattt acaaaaagag cttttcttaa cggcagaatt gatttaactc aggcagaagc
     2941 agtaattgac ataataaatt ccaagaccga atcaagtcat gaaatttcaa taaaacatct
     3001 ggaaggcaga ctttcaaaac aaattaatgc agttatagac gaaataatgg aattgttggc
     3061 caacattgaa gttaatattg attttcccga atatgacgag gatgaaataa ctattgaaaa
     3121 agttaaagct ttatgtcata acttagtacc tgagttggat aaacttataa atacggctga
     3181 taccggaaaa ataatcaagg aaggtataaa aacagtaata cttggaaaac cgaatgtagg
     3241 aaaatcgtct cttatgaatt ttcttttgaa tgaaaatcgt gcaattgtta ccgaagtacc
     3301 gggaacaaca agggatacta tagaggaata cgtgaatatt aagggcgtgc cgttaagaat
     3361 aatcgatact gcaggtataa gagaaaccga tgatatggta gaaagaatag gtgttgaaaa
     3421 agctttagaa aaggtaaagg aagccgacct tgtaataatg atttttgatt catcaaaaga
     3481 gcttgaaaaa gaggatgaaa gtatattaga ttatattaaa gataaaaagg ttatatatat
     3541 aaggaataaa actgatttgg aaagcaggct ggatttgtct tcttataaaa aaatagaaga
     3601 agagattatc aatgtatcta ttttacaaaa tcaagggctg gatgatatca ttaataaaat
     3661 ttactcttta ttttttaaag gccggttaga tataagggat gagcttatta ttaacaacgt
     3721 aagacataag aatttattga taaaagcaaa aaacaacatt gaagaagtgt taaaatcaat
     3781 tgaaaacaat atgcccattg attttatcga gatagattta atgcaagcaa tggaaaattt
     3841 gggattgatt gtaggtaaat cggtcagcga tgatttaatt gacaaaatat tcgatgaatt
     3901 ttgcatagga aaataggaaa ggaacaacaa tggaagacag agtaagagaa tttctcgagg
     3961 agagcattga tgtgttagta attggtgcag gtcatgcagg atgtgaagca tccttggcga
     4021 ctgcacgtct tggtttaaaa acaataatgc tgactttaag ccttgattca atagcgatga
     4081 tgccatgcaa tcccaatatc ggggggacgg caaaaggaca cttggtcagg gaaattgatg
     4141 ctcttggcgg agaaatggct ttaaatatag ataaaactat gatacaatgt aagatgttga
     4201 accgctcaaa aggacctgcg gttcattcct taagagccca agccgacaaa aaaaagtatc
     4261 atagggaaat gaaaaaggta atagaaaatc agaaaaattt aagggtaata caaacagaag
     4321 caactaagat aaaagtaaat gaagataaga gctttaatgt atataccaag cttggagcat
     4381 actacagggc aagagcggtt atagttacta caggcacgta tttaaaggga agaatttata
     4441 tgggtgaatt gaactattca tcgggacctg atggtttggc tcctgcaaat gaattgtccg
     4501 attcattgat ggacttagga attgagctta taaagtttaa aacgggaaca cctgcaagaa
     4561 tacacaagga ttccattaat tatgaaaaaa tgacaataca gaccggtgat gatgaaataa
     4621 tacctttctc atttatgaat gatgaaataa acattgagca ggtaccgtgc tatattacct
     4681 atactactcc gcaaactcat gaattaataa ggaataattt agacaggtcg ccactttacg
     4741 gcggtgttat aaaaagtgtg ggaccaaggt actgcccatc attggaagat aaggtagtta
     4801 aattttcaga caaggaaaaa catcaacttt ttgtagagcc tgaaggtctt gatacaaaag
     4861 aaatgtatat acagggaatg tcaacttctc tgccttacga ggttcagatt caaatgtatc
     4921 gaacaatgat aggacttgaa aatgctgaaa ttatgagacc tgcttacgct atagaatatg
     4981 atgccatcga ccctacccag ctcaagccga gtcttgaatt aataagtatt aagaacttat
     5041 ttttcgcagg tcagataaac ggaagctcgg gatatgaaga agctgcggca caaggtttga
     5101 tagccggaat aaatgcatat ttaagcttaa acaataaaga gccttttatt ttagaccgct
     5161 cggaagcata tataggagtg ctgattgatg atttggttac caagggaaca acagagcctt
     5221 acagaatgat gacatcgaga gcagagtaca ggcttcttct aagacaggac aatgctgatg
     5281 aaagacttac ggaaaaaggc tacaaaatag gattagtaac agaagaaaga tataaaagat
     5341 tcaaagataa aagacaaaga attgaaaatg aaatcaatag attaaaaaat cttaaaataa
     5401 caccaaaggc tgatactaat aaagctttgg aggatatggg aagcgctgct ttaaaaattc
     5461 cgacaacact ttatgattta ttgagaagaa cagagctgaa ttatgacaac accttgttct
     5521 tggatccaaa gagacctgta ttgagaaaag aaataaagga ttatgtggaa acagttataa
     5581 aatatgaagg atatattcaa aagcagatag cccaggtaga gcaatttaaa aagcttgaaa
     5641 acaaaaaaat tccggaagat attaactacg atgatgtaaa aagtctcaga attgaagcaa
     5701 gacagaaact aaacaaaatc aggcccaact ccattggtca ggcttcaaga atttctggtg
     5761 tttcgcctgc cgacataaat gttttgttaa tatatttaat gacttacaat aaagaataaa
     5821 ggtgagcttg tgataaaaac attggaagag ggttttaaag agcttaaatt accctacagc
     5881 aaagatattg aaaataattt cattatttac agagatttat taaaggaatg gaataaaaaa
     5941 ataaatatta cttccataga aaatgatggg gaaatttata taaagcattt tattgatagt
     6001 gttcttttgt tgaatgaaaa taatataaat gaaaacaaaa ctataattga cgtaggtacc
     6061 ggaggcgggt ttccggggct tccgcttaaa attgttaata ataattataa aattacgctt
     6121 ttggacagtt tgagaaaaag aatagatttt ttgtccgaag tgataaaagc tttaaattta
     6181 aatgatgtga aacttattca cggcagagct gaagattacg gccaaaataa ggaatataga
     6241 gaatgttatg atatttgtgt atcaagagct gtagcccctc ttaatgtttt gagcgaatat
     6301 tgtattcctt ttgtaaaagt aggaggatat tttgcagcat acaaaagcga caatatttcc
     6361 caggaaattt caagttccga caatgcaatt aagaaacttg gcggagaaat taaagaaatt
     6421 aaaaaaatta atcttccggg tactgatatt ataagaaaaa ttgttataat agagaaaaca
     6481 gacaaaacag aaatcgagta tccgagaaaa gcaggaaagc cggccaagga tccgttaaaa
     6541 taagggggag gaaatgatta gcaatattgt tcatatcgat gtcaataaaa taattccaaa
     6601 caaaaatcag ccaagaaaaa cttttgatga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnnn ataagccaga cacagttggc tcaaaaattg ggcaaatctc aatcagcaat
     6781 tgcaaacaaa gtaagaattc taaaactgcc ggagtcggtt aaagaaaaat taagagaagg
     6841 aaatttaacg gagcgccacg gtagagcctt gttaaaaata aatgatgaag aaattcttct
     6901 taatattgtt gaaaaaataa ttaaaaagga tttaaatgta agtgaaaccg aaaaaattgt
     6961 ggattcaata gctgaagagt tgaatattaa gaaaaaagcg gacaagcgtt atgtaaggaa
     7021 ttttataaat tataaaatat atataaatac aataaaaaac gcttacaatg aaatagtaaa
     7081 aacaggtatt gatgctaaat ttgagcagaa cgaatcagat gattacattg aaataaaggt
     7141 gaaaattcca aaaaaagcat gtagagggtg atgatatgac caaagtcata agtattttca
     7201 atcaaaaagg cggtgtgggg aagacgacaa ccaatgttaa tttatgtact gcattggcgt
     7261 taaaaggaaa gaaagtactt agtattgata tagaccctca gggaaacaca accagcggat
     7321 tcggatttaa caaaaataaa ctggaatata caatttacaa tgtattgatt gaagattacg
     7381 acatcaacaa aattattttc aagacggaaa tagaaaacct ggatattact cccgcaaata
     7441 ttcagcttgc aggtgctgaa attgagctta ccaatactaa attcagggaa aaaacattaa
     7501 gggaaaaaat aaatttaatt gaaaacaaat atgattttat tatggtggat tgtccgccct
     7561 ccttggggct attgtcatta aatgctctta cagcatccaa taccgtactt ataccgatac
     7621 aatgcgaata ctattctttg gaaggagtag gacagcttat tgatactgtt aagcttgtaa
     7681 agaaaaattt aaatcctaag cttgaaatcg aaggcgttat tttgaatatg tttgacggca
     7741 gaacgaatct ttcgatacaa gttgttgagg aagtcaaaaa atattttaag gataaagtat
     7801 atagaaccgt aataccgaga agtataaggc ttgccgaagc accaagcttt ggacagccta
     7861 taatgctata tgacgataaa tcaaaaggaa gcattgcata tataaaatta tctgaagaat
     7921 ttataaaaaa taattgagga gagaagatag tatgtctgca aagagaaaag cattaggaaa
     7981 aggcttgtcg gcattaatac ccgaagattt agaaaaggaa gattatgaaa aaatccatga
     8041 aatagatatt gatttgattt ccccaaaccc aaatcagcct cgcaagcttt ttgaacagga
     8101 taagttagat gaacttacgg aatccataaa aaaatatggt gttattcagc ctataatttt
     8161 aagaaaagac ggtgagctct acactataat tgcaggagaa agaagatgga gagcttgcaa
     8221 aaatgcaaat ataaaaacaa taccttctat agtaagggat atagaaaaca aaaatgcctc
     8281 tgaaatagca ctgattgaaa atattcaaag ggaagattta aatccgatag atgaggcaaa
     8341 tgcttatcaa tttattatgg acaggtacgg cataactcag gaacaggtgt caaacatagt
     8401 aggaaagtcc agagtatatg tgactaatat aatgagactt aataatctag atgaatatgt
     8461 gaaggacaaa attattaaaa acttaattac tgcgggtcac ggcagagcaa tactaagctt
     8521 ggacaaagaa aaacaaaaat ccctgacgga tattataata cgagataatt taagtgtacg
     8581 agatgtagaa aagttagtac gctcttcgaa aaaaccgcaa cgcagagaaa agcccgcaaa
     8641 agataaatat atagtatatt tggaggaatt gctgacagaa agattttcaa caagggttaa
     8701 tatcaaaaac aaaaaagata aggggaaaat tgaaatagaa tacacgagca gtgacgattt
     8761 aaacagaata ctggatttat taaaaataga agattgagta tgacctaacc caccggatgg
     8821 tgcatcctca tgcgccgtcc taccatctgt gatgctttga cactatttat acatttccac
     8881 ccatcatatc attatatcat tataactatg catttccaac ctttcccaac gtttgttaat
     8941 tattagacct atgttttttt attaatattt atattgaata tacatattta taatttgcat
     9001 ataagatata atttctttta gtaaaaataa tgcgaaaaaa gtcaaaattt gattaattat
     9061 ggtaatcatt tcattagatt tgcattcttt taagttaatg tttttgaaat caattgacaa
     9121 ttaattaacg aaataatata atatttataa atatcaagaa tgcaatgagg aggttattat
     9181 tttgacaaaa gcactggatg taaaggcaaa tgaaaagctt tttattgagc ttaaggagta
     9241 cattgattca gttaaggatg tggacggatc cttaatgcag gtattgcata aagctcagga
     9301 tattttcgga tatttgccta tagaagttca aaaatttatt tcttccgaat tagatgttcc
     9361 gttagctgaa gtgtatggag ttgccacatt ttatacacaa tttgccattg aacctaaagg
     9421 gaagcacaaa attggagtat gtctcggtac agcatgctat gtaaaaggcg cgcaattgat
     9481 tatggacaaa ctgacaaaag aattaaatgt taaggtaggt ggaactacgc aggataattt
     9541 atttactttt gaagctactc gctgtctggg ttgttgcggc cttgctcctg taatgatgat
     9601 agaccaagac gtctacggaa aattagagcc gaaaaaaata cctgaaattc tggcaaaata
     9661 taagtagggg gttattatga aatcattgga agaattaaaa aagttaagag atgaatcaat
     9721 taagaatatt gaaatgcgaa ataccgataa ggatgtgcgt gttgtggtag gcatggcaac
     9781 ctgcggaata tctgccggtg caagacctgt tttagccaca tttgtagaag aagtagcaaa
     9841 acgaaatttg aacaatgtgt cggttgtaca gaccggttgc ataggaatgt gcacttatga
     9901 gccaatagtt gaaatctatg ctccaggtaa ggaaaaggta acttatattc atgttgactc
     9961 tgaaaaagct aaaagaattg tatccgaaca tttggctaat aacagagttg tagttgaata
    10021 tacaataggc tcagaaaatt aacggtagaa agtgaggatg gttatgaaaa ttttcagatc
    10081 tcatgtgttg gtttgcggtg gaacgggctg tagttcttct aattctgctc aaattagaca
    10141 aagatttatt gataaaataa aagaagtaaa tttagataat gaagtacagg ttattggtac
    10201 cggctgcttt ggtctttgtg aagtgggtcc ggtagtaatt atatatcctg aaggttcatt
    10261 ttatgctcga atgactgttg acagagttga tgaaatagtt gaagagcact tattaaaagg
    10321 aagaatagta aaaaaatact tgtatgatga aagtataaag gatgaaaaac taaaagcttt
    10381 aaacgaagtt gatttttaca gtaaacaaaa gagagtcgca cttcgtaatt gcgggctaat
    10441 aaatcccgag aacattaacg agtatatagc aagagacgga tatatggcac ttggcaaggt
    10501 tcttacggaa atgactcccg atgaagttat taaattagtc aaggattcag ggttaaaagg
    10561 aagaggcgga gccggtttct cgacaggaat gaaatggagc tttgcagctc ccaacaaggc
    10621 agaccagaaa tatataatat gcaacgctga cgaaggtgac ccgggagcat ttatggacag
    10681 aagtgttttg gaaggtgacc cgcatgcagt attggaagct atggcaatag caggttatgc
    10741 aattggagct acaaaaggat atatatacgt aagagcggaa tatcccatag cagttcgcag
    10801 attgaaaatt gcaatagaac aagccaggga attaggtctt ttagggaaaa acatattcaa
    10861 tacagg
//
LOCUS       DAOSOW010000008        10856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_6044_length_10856_cov_3.854088, whole genome shotgun sequence.
ACCESSION   DAOSOW010000008 DAOSOW010000000
VERSION     DAOSOW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 10856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10856
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6044_length_10856_cov_3.854088"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLD67_00575"
     CDS             complement(<1..491)
                     /locus_tag="PLD67_00575"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOT21052.1"
                     /translation="MESENIIIRKSTFDDCKIFTEWEQQDYVKEFLTINKERTYEEVV
                     REFILREQDPTKEQYTIILKNTGPIGRVYLSNISREFDFIDITRIYIGEKEHLGKGYG
                     RECMELLLDYCFNILKMERVTLDHYTGNKASNLYLKLGFKYEGIMRHAAKKDGKYYDL
                     HLMS"
     gene            complement(536..1054)
                     /locus_tag="PLD67_00580"
     CDS             complement(536..1054)
                     /locus_tag="PLD67_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOT21053.1"
                     /translation="MLLILALLAHEVSHVFSAILLNIEFSKVKITLFGINLNAHLDNA
                     SHAKKIILFFAGPLCNLCFYFGFRNTEYFCFANMNLLLAYINLFPIVPLDGGNICKTI
                     LEIFLDTKTVSGYITMTNAFFILFFIIIIYIFKDYLFFLLVVMGLKGIVEENSYLLEK
                     SIIAVYNKINTK"
     gene            complement(1068..1691)
                     /locus_tag="PLD67_00585"
     CDS             complement(1068..1691)
                     /locus_tag="PLD67_00585"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT21054.1"
                     /translation="MNKLTKKRLNIKRKEQVNKKLVLQVIFSIVLVAAVIVTKNINSN
                     LSEKIIEAADEKMSASIELLEVRDTIAKGFTTAINKLPFVGKEEASPVIGTLYRKYGL
                     NKTDDVSYYNHGIDIKSETQSVKALTDGKVMSVGKNEKHSGYIVIQDEDRTLIYGKIN
                     ETFVKEGDNISKGDVIGALDEENMILHIEVWEDGASINPSSLFDLTK"
     gene            complement(1834..2517)
                     /locus_tag="PLD67_00590"
     CDS             complement(1834..2517)
                     /locus_tag="PLD67_00590"
                     /inference="COORDINATES: protein motif:HMM:NF025001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT21055.1"
                     /translation="MNVILTGSQKNNIGKTIISIKLAIELANSGKNVLMMDLSSGKIK
                     MSEYLNVNEDIIYDIVDVVNKTCTLEQGIIEINENLSLLPCPRIAGKINEIKKESFIN
                     LLQSVEVYDYVIIDADKLTSVFIDFSIINNAITINNNDFSCIKEINTDKTISSNAGNF
                     IVAINKYNKKKAKKGIMMKSNDIEKLTETTISALIEENIKYSDLNHEKFLDDNFLKTE
                     IKNIVKNLK"
     gene            complement(2553..5885)
                     /locus_tag="PLD67_00595"
     CDS             complement(2553..5885)
                     /locus_tag="PLD67_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT21056.1"
                     /translation="MIKFFKNRFNILYLIIVLLLIILGFRMAVLTIVEGQEYRQIADI
                     KKIKDIPVKAPRGKIFDRNGIILADNVSSFTVQMYTDEIKPEDFNEIAYKLSKILDEN
                     MERPIDDFPIELDTFDYINESVEIIDSDLNLEEEIEVNYYRTAEEKVIEKVKENIHEW
                     LNFSISIYGENFSPKQRVHAAIINDNVPVKLEGDKFEYEDEMLIQTDSDLTEAENETT
                     EQKTPIQIWLSEHNLKENISAEEAVAQLLVNNNKYLSSLFSNSKIRRLSYEFIIQKGV
                     ENIKLNEFSFIQDEEYENIKRDLNALNEEITMDTSAKDDFVILTMKNSIDNLLQTVYQ
                     GNEKIMPGAILIERLKEIYPDLPVKFVENGELGVFEYTDTETRNKYFNQQHMEYSTTA
                     YDFIKEIAFKNYDVLYNLVTDDNIKYYSQIELLKYVNPSISINEWEYTPLRNKKNWIN
                     NNVEFDEETDDYSAKKVFEMLRESLKIPEEINDYDMRNMMVIRERYNIKSYLSYHPVD
                     ICYSISQKTVAMISERHHELNGINIEIEPLRYYPQYESASHIIGYLGKIAQDYELHEY
                     VVEKGYSTDDIIGKTGLEEKFEDYLRGDKGKKTVEVNNVGRTIRSVSSEAPVPGDDLY
                     LTLDLRLQKKAEESLKKGLEKIREGGVFESEWGDFQFRDKYENAYSGALVALDVKTNE
                     VLALANYPAYDLNLFSTGITTENWNSLLNDSKNPLAPRPLYNIAMLTAIQPGSTFKMI
                     TSLAALEKGVNPNTTVYCAGTMKVGDRDFRCWIYNMFGGRHGSQTMYQAIMNSCNFYF
                     YTTVLGENLATHQKHTVKVDAEDIIEMASKFGLDSKTGIEIDIPQEASGGVPSIEGKK
                     SGIRVYLRLFLEANIERYLNDGVVIDASMKNEIIEEIASWIDRDELMTRAEVYEGLLA
                     LNLNPDKTNDNYVPLVDIIKYSYLNQATWTVGDNLNISIGQGNNAYTALQMANYVSSI
                     ASGLRRNVSVVKEIKTYDGKKTDYIPLRESEKIELSDDSYLDIVKHGMKLVSYDDSAK
                     PFAIFPVEVGSKTGTAQNQGINPETGQPYDDFAWYVAFAPYDDPQIAVACVLFEGGSG
                     RYPIPIVREVIGEYLKINGLIE"
     gene            complement(5892..6386)
                     /gene="mreD"
                     /locus_tag="PLD67_00600"
     CDS             complement(5892..6386)
                     /gene="mreD"
                     /locus_tag="PLD67_00600"
                     /inference="COORDINATES: protein motif:HMM:NF016019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOT21057.1"
                     /translation="MKKLILMSSLIVIGFVFQTSFIGFFNYFKILPNIPLILLVIFAM
                     LSDGVEGGVLGILTGILHDAMIYDVFGIYTLIYFIIGAVIGAFSDEMLRENKLVYSAV
                     TGISTVVMHLLLFLILFFLRFRVHLAKSILPNILFETALNAVLVIFVLKFVIYIFDRF
                     NVKA"
     gene            complement(6406..7239)
                     /gene="mreC"
                     /locus_tag="PLD67_00605"
     CDS             complement(6406..7239)
                     /gene="mreC"
                     /locus_tag="PLD67_00605"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOT21058.1"
                     /translation="MNFLKEHFKKILFFLTIFILIIMIGLSSIGRFEGADLGFIGNLI
                     AGFQKLTYKTGNTLTNSFYSVQDIVKMREENIMLTESIYKLREQVRILENIINKSEAL
                     ETEYEMRKNLTYDYVVGQVIAIDDSNWFSRFTIDKGENDGIRQNDIVIQAVKTDDGIV
                     QIGLLGIVTQTSSDWAKVITLLDEGCKVSFKNIENDESGIVQGSIDGNVTGYFFNSKA
                     GAEKGDNLVTSGIGEIYIPDIYIGDVKDVVKTTDASTLRIIVEPAVDFTKISRVYVLT
                     K"
     gene            complement(7288..8439)
                     /locus_tag="PLD67_00610"
     CDS             complement(7288..8439)
                     /locus_tag="PLD67_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003367424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOT21059.1"
                     /translation="MGLFDFFTQKIGIDLGTSNTLVYVNNKGITLNEPSVVAINTTSY
                     EVCAVGFDAKAMLERTPKTIRAIRPLQNGVIADFEITRAMIKYFITKALNKKRFIKSN
                     IVISVPVGVTSIERRAVEEVAYDAGARKVKLVEEPMAAAIGAGLNVDQPIGNMIVDIG
                     GGTTEIAVISLGGIVESDSLRIAGDDIDINIKEYIKNKYKMDIGIVTAENIKKVLGNA
                     SKEYYKRIIDSDERIDSKEANEDNEDFEINEDNKDKKDLKDKKDKKEKKDEKENKKTD
                     YVQQMEIRGRDIVSGLPLKITITSDEVREAILETVNSIVNAIKRVLERTPPELSSDIY
                     TNGIYLSGGGALLKGLDIYLEKETGIKVFIADNPLQSVALGAGKISEEL"
     gene            complement(8457..9146)
                     /gene="radC"
                     /locus_tag="PLD67_00615"
     CDS             complement(8457..9146)
                     /gene="radC"
                     /locus_tag="PLD67_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOT21060.1"
                     /translation="MDNTNYTIKSLPADDRPQEKLLKYGAGALSNSELIAVILRTGTR
                     EENVVMLSQRILKEGGKGLRNIAEGSLEKFKSYKGISDAKAAKLMALAEISKRMSTLK
                     IEKLKISSPDDAAVMMMEEMRYYQKEYFKIMLLDTKNNVKKVSEISVGSLNSSIVHPR
                     EVFYEAVVNLASSIILVHNHPSGDCEPSHEDIVLTNRLDESGKILGIRVLDHIIIGDG
                     VYFSFKEEGLI"
     gene            complement(9173..9748)
                     /locus_tag="PLD67_00620"
     CDS             complement(9173..9748)
                     /locus_tag="PLD67_00620"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOT21061.1"
                     /translation="MKKIVLASNSPRRIEMMKKYVDIITYIPDIVEKVNSFDYPQTTV
                     MKVAFEKALNVYLKYKENALIIAADTIVYLDEIMGKPKDEAEAYKMLSLLSGKTHYVF
                     TGICIIDTESSKKIVDYEKTQVVFNELTEGLIKRYLKTYEYLDKAGAYGIQGYGELLV
                     KEIHGCYNNVKGLPIAKLNNLLSKHFSYDLL"
     gene            complement(9757..9999)
                     /locus_tag="PLD67_00625"
     CDS             complement(9757..9999)
                     /locus_tag="PLD67_00625"
                     /inference="COORDINATES: protein motif:HMM:NF025572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HOT21062.1"
                     /translation="MRKGNSFYLILLILTSVIIGTILGKAFSNMLPILNYGEGIGFGP
                     ATLDLNIFTITFGFSARLTVAGIIGLIAGIIIFRKL"
     gene            complement(9999..10520)
                     /locus_tag="PLD67_00630"
     CDS             complement(9999..10520)
                     /locus_tag="PLD67_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOT21063.1"
                     /translation="MNKIHRYIFLSLLTAAALILSIIEGMMPLPYIAPGAKLGLSNIV
                     ILSTVVVFGFKDALLVVIIRSILLMLVATNPITFIYSIASGIVSTIVMSLACKYLSNI
                     FSLIGISVLGAMAHNTTQVTVAAILFTTVNLYYYLPILSLVSIFTGCFVGYTAIFVTN
                     NLETTVKTWRRGV"
     gene            complement(10533..>10856)
                     /locus_tag="PLD67_00635"
     CDS             complement(10533..>10856)
                     /locus_tag="PLD67_00635"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOT21064.1"
                     /translation="FILYGGRDAVISNSKTAYIYSNNKLVGEYVLTDDYKDVVNIESE
                     TGYNIMHIENGQIWIHEASCPDKICIYQGKISKNGEMIVCIPNRMFIKIVDENDESEI
                     DFIAD"
BASE COUNT         3171 a         2147 c         1462 g         4076 t
ORIGIN      
        1 gacattaagt gcaaatcata gtatttaccg tctttttttg cagcatgtct cattattcct
       61 tcatatttaa aacctaattt caaatacaga ttactagctt tatttcctgt ataatggtct
      121 aatgttaccc tttccatttt taaaatatta aagcaatagt ctaagagcaa ttccatgcat
      181 tctcttccgt aaccctttcc aagatgctcc ttttctccga tatatatccg tgtaatatca
      241 atgaagtcaa attctcttga tatattagaa agatagactc ttccgatggg tcctgtgttt
      301 ttcagaataa tcgtatattg ctcctttgta gggtcctgct ctctcaaaat aaattctcta
      361 acaacttcct cgtatgtacg ctctttgttt attgtcaaaa attccttaac ataatcttgt
      421 tgctcccact cggtaaatat tttgcaatca tcaaaagtac tttttctaat tattatattt
      481 tcagattcca ttttatcctc cggctatttg gatttaagag ttgtttaggg ttatttcatt
      541 ttgtatttat tttattatat actgctataa tggatttttc aagcaagtaa ctgttttcct
      601 ccactatccc ctttagtccc ataacaacca gcaaaaaaaa caaatagtct ttgaatatgt
      661 atattataat tataaagaaa agtataaaaa aggcatttgt catagttatg taaccggata
      721 ccgtctttgt atccaaaaaa atttctaaaa tagttttgca aatatttcct ccgtctaaag
      781 gcacaatcgg aaataaattt atgtatgcca ataacaaatt catatttgca aaacaaaaat
      841 attctgtatt tctaaatcca aaataaaaac acagattaca taaaggtcct gcaaaaaaca
      901 aaataatttt ttttgcatgt gaagcattgt ctaaatgtgc atttagattg attccgaaca
      961 gtgtaatttt aactttagaa aattcaatat tcagtaatat tgcagaaaat acatgggaaa
     1021 cttcgtgtgc taagagagct aagattaaaa gcatttccca aaggtgttta ttttgtcaaa
     1081 tcaaataatg atgaaggatt tattgaagca ccgtcctccc aaacttcaat gtgaagaatc
     1141 atattttctt cgtcaagggc tcctataaca tctcccttgg atatattatc cccctcttta
     1201 acgaaagttt catttatttt cccgtaaatc aatgttctat cttcatcttg tattacaata
     1261 tagcccgaat gtttttcatt tttgcctacc gacattactt ttccgtccgt aagagcctta
     1321 accgactgtg tttcggattt aatgtctatt ccatgattgt aatacgatac atcgtctgtt
     1381 ttgttcagtc cgtattttcg ataaagtgtc cctattacgg gtgaagcttc ttctttacct
     1441 acaaaaggta atttgtttat tgcagttgta aaaccctttg caatagtatc tcttacttcc
     1501 aataattcaa tgcttgcact cattttttca tctgctgctt ctataatttt ttctgaaaga
     1561 ttcgagttta tattctttgt tacaataacg gctgctacca aaactatgct gaaaattacc
     1621 tgtaaaacta atttcttatt aacttgttcc tttcttttaa tattgagcct tttttttgtt
     1681 agcttattca tttcaccacc ccttccaata cattttatta atgtgttaca agctttatta
     1741 caaaataaaa aaacttttgt ccctgtatta ccagtgacaa aagttaatat ttcataatcc
     1801 ttcactattc ctataaaaac agaggaaagc aggttatttt aaattcttta ctatgttttt
     1861 gatttctgtt tttaaaaaat tatcatcaag gaacttttca tgattcaaat ccgaatactt
     1921 tatattttct tcaattaaag ctgaaattgt tgtttcagtt aacttttcaa tatcattgct
     1981 cttcatcata attccttttt tagctttctt tttgttgtac ttgtttattg caacaataaa
     2041 gttaccggca ttagaagaaa ttgttttatc cgtatttatt tcctttatac atgagaaatc
     2101 attgttattg attgttattg cattattaat tattgaaaaa tctataaata cggatgttaa
     2161 tttgtctgcg tctattataa cataatcata gacttctaca ctttggagta agtttataaa
     2221 tgattctttt tttatttcat ttattttgcc ggctattctg ggacatggca acagcgataa
     2281 attttcgtta atttctataa taccctgttc caaggtgcaa gttttattta caacatcaac
     2341 tatgtcataa ataatatctt cattgacatt caagtattct gacattttta tctttcccga
     2401 ggacaaatcc atcattagaa catttttgcc tgagtttgca agctcaattg ctaactttat
     2461 acttattatt gtcttgccta tattgttttt ttgtgaacct gttaatataa cattcatttt
     2521 ttatccccct ataacgacta ttctgcactt tgtcactcaa tcaaaccgtt tatctttaaa
     2581 tactctccta taacttctct cactatcgga ataggataac gtcccgagcc tccttcaaaa
     2641 agtacacatg ctacggctat ttgcgggtca tcgtaaggtg caaatgcaac ataccacgca
     2701 aaatcatcat agggctgtcc tgtttcagga ttgattcctt gattttgagc cgtaccggtc
     2761 ttgctgccta cctcaacagg gaagattgca aatggttttg cactgtcatc ataagaaaca
     2821 agcttcatcc catgttttac aatatctaaa tagctgtcat cggacaattc aattttttcg
     2881 gattccctaa gcggaatata atctgttttc tttccgtcat aagtttttat ttcttttact
     2941 acgctgacat ttcttcttaa gccgcttgct atggaagaaa cgtaatttgc catctgcaat
     3001 gctgtatagg cattatttcc ctgacctatg ctaatattta aattatctcc cactgtccat
     3061 gttgcttggt ttaaatatga atattttatt atatcgacta agggtacata attatcgttt
     3121 gttttatcgg ggtttaaatt taatgctaat agcccttcat agacttcggc cctggtcatc
     3181 agctcatccc tgtctatcca gcttgcaatt tcttctataa tttcattttt catcgatgca
     3241 tctataacaa ccccgtcatt aagatatctt tcgatgtttg cttctagaaa taatcttagg
     3301 taaacacgaa ttccggattt tttaccttct atgctcggaa ctccgcctga agcttcctgg
     3361 ggtatgtcaa tctctattcc tgtttttgaa tctaatccaa acttgcttgc catttctatt
     3421 atatcctctg catcaacctt cacggtatgc ttttggtgag ttgctaaatt ttcacctaag
     3481 actgttgtat aaaagtaaaa gttacaggaa ttcattattg cctgatacat tgtttgagag
     3541 ccgtgccttc ctccaaacat gttataaatc cagcatctaa aatctctgtc gcctactttc
     3601 atggttcctg cacaatacac cgtagtatta ggatttactc ccttttccaa ggcagccaag
     3661 gatgtaatca ttttaaaggt ggagcccggc tgtattgccg ttagcattgc aatattgtaa
     3721 agcggtcttg gagccaaagg gttttttgaa tcgtttaaca ggctgttcca gttctctgtg
     3781 gtaattcccg ttgaaaacaa attcaagtca tatgccgggt aatttgccaa agccaagact
     3841 tcatttgttt taacgtcaag tgcaaccaat gctcccgaat aggcattttc atatttgtct
     3901 ctaaattgaa aatctcccca ttcgctttca aatactccgc cttcacggat tttttcaaga
     3961 cccttcttca aagattcttc tgcttttttc tgcagcctaa ggtccaaggt taaatacaga
     4021 tcatcgcctg gaacaggtgc ttcgcttgat actgagcgga ttgttcttcc cacattgttt
     4081 acttcaactg tttttttacc cttatctccc cttaaataat cctcaaattt ttcttcaagc
     4141 cccgtcttgc ctattatgtc atctgtacta tagccctttt caacgacata ttcgtgcaat
     4201 tcataatcct gagcaatttt ccccaggtaa cctataatat gggaggcgct ttcatactgg
     4261 ggataatacc ttagcggttc aatttctata tttattccgt ttaattcatg atgtctttca
     4321 gaaatcattg caacggtttt ttgtgaaata ctatagcaaa tatccacggg gtggtatgaa
     4381 agatagcttt ttatgttata cctttccctg attaccatca tatttcgcat atcgtaatcg
     4441 ttgatttctt caggtatttt taaggactct ctaagcattt caaatacttt ttttgctgag
     4501 taatcatcag tttcttcatc gaattctaca ttgttattaa tccagttttt cttgtttcgc
     4561 aagggtgtgt attcccattc gtttattgat attgaaggat ttacatattt taaaagctct
     4621 atttgggaat aatattttat gttatcatcc gtaaccaaat tgtataagac atcataattt
     4681 ttaaaagcta tttcttttat aaaatcatag gctgtagtgc tgtattccat atgctgttga
     4741 ttaaaatact tgttccgggt ttctgtatct gtatattcaa atactccaag ttcaccgttt
     4801 tctacaaatt taaccggcaa gtcgggataa atttctttca gtctttctat aagaattgct
     4861 ccgggcataa ttttttcatt tccctgatat actgtctgaa gcaaattatc tatagagttt
     4921 ttcatggtca aaataacaaa atcatctttt gccgatgtgt ccatagtaat ttcttcattc
     4981 aatgcattta aatctctttt gatgttttca tattcttcgt cctgtataaa tgaaaactcg
     5041 ttaagcttaa tattttctac tcccttttga ataataaatt cataggaaag tctccttatt
     5101 tttgaattgg aaaaaagtga agataaatat ttattgttgt tgaccaaaag ttgtgccact
     5161 gcttcttcag cacttatatt ttcttttaaa ttatgttctg aaagccaaat ttgtatggga
     5221 gttttttgct cagttgtttc attttctgct tcagttaaat ccgaatcagt ttgtataagc
     5281 atttcatcct catattcaaa tttatcgccc tctaatttta ctggtacatt atcgtttata
     5341 atggctgcat gaaccctttg tttgggagaa aaattctcac cgtatataga aatgctgaag
     5401 ttcagccatt catgaatatt ttccttgact ttttctatta ctttttcttc agcggttcgg
     5461 taatagttta cttcaatttc ttcttcaaga tttaaatcgc tgtcaattat ttcaacactt
     5521 tcatttatat aatcaaatgt atcaagctca ataggaaaat catctattgg tctttccata
     5581 ttttcatcta atatttttga cagtttatat gctatctcat taaaatcttc aggttttatt
     5641 tcatccgtgt acatttgcac ggtaaagctg gacacattgt cggcaagaat tatgccgttt
     5701 ctgtcaaaaa ttttccccct gggtgcttta acggggatat ccttaatttt ctttatatct
     5761 gcaatttgtc tgtattcctg tccttcaaca atggtgagta cagccattct aaaacctaaa
     5821 atgataagca acaaaactat tatcaagtat aatatattaa agcgattttt aaaaaacttt
     5881 atcatttcac ctcatgcctt tacattaaat ctatcaaaaa tatatataac aaattttaac
     5941 acaaatataa caagcacagc atttaatgct gtttcaaaaa gtatatttgg caatatactt
     6001 tttgctaaat gtactctgaa tcttaaaaag aataaaataa gaaataataa taaatgcatc
     6061 actacagttg aaataccggt tacggcactg taaacaagct tattttctct gagcatttca
     6121 tcgctgaagg ctcctattac tgcgcctata ataaaataaa tcaaagtata tattccaaag
     6181 acatcataaa tcatggcatc atgcaaaatc ccagtcagaa tccccagaac tcctccctct
     6241 accccatccg ataacatggc aaatatcacc aataaaatca gcggaatatt tggaagaatt
     6301 ttaaaatagt taaaaaagcc aataaatgat gtttggaata caaaacctat aactattaag
     6361 gaagacataa gaataagctt tttcattatt aagcctttct aatcattatt ttgtaagtac
     6421 atatactctg cttatctttg taaaatcaac agcaggctcc acaataattc ttagggtaga
     6481 agcatctgtt gttttaacaa catcttttac atcacctata taaatatcag gaatataaat
     6541 ttctcctatt cctgatgtaa cgagattatc acctttttca gcaccggctt tgctgttgaa
     6601 aaagtatcct gttacattgc cgtcaatact tccctgtaca attccgctct catcgttttc
     6661 aatatttttg aagcttacct tacagccctc gtctaaaagt gtaataacct tagcccaatc
     6721 ggatgaagtt tgagtaacaa tgcctaaaag acctatctgt actatgccgt catccgtttt
     6781 cactgcttgt ataactatgt cattttgtcg tataccgtca ttttctccct tgtcgattgt
     6841 aaatctgctg aaccaattag aatcatcaat agcgattact tgtccaacaa catagtcata
     6901 agtaaggttc ttcctcattt cgtattccgt ttccaaagct tcggacttat tgataatatt
     6961 ctccaatatc cttacttgct ccctgagttt ataaatactt tcagtgagca ttatattttc
     7021 ttcacgcatt ttcacgatat cctgtacgga atagaatgaa tttgtaagtg tattgcctgt
     7081 cttataggtg agcttttgaa agcctgctat caagttgccg ataaagccta aatctgctcc
     7141 ttcaaatcgt ccgattgatg acagtcctat cattattatc aatatgaaaa ttgtaagaaa
     7201 aaataatatt ttcttgaaat gctctttcaa aaaattcatt tgctcaccgc tttcaaacat
     7261 cagaagaaat tgccacgcaa tttctcgtta taattcttca ctgattttgc ctgcacccaa
     7321 agcaacactt tgcaatggat tatctgcaat aaatactttt attccggttt ccttttcaag
     7381 atatatatcc aagcctttta acaacgctcc cccaccgctt aagtatattc cattagtata
     7441 aatatctgaa gataattcgg gaggtgttct ttcaagaacc ctttttatgg cattaacaat
     7501 tgaatttacg gtttcaagaa tagcttctct cacctcatcg cttgttattg ttatttttaa
     7561 cggaagtccc gacacaatgt cccttcccct tatttccatt tgttgaacat aatcggtttt
     7621 tttattttct ttttcatctt ttttttcttt tttgtctttc ttgtctttta aatctttttt
     7681 atctttatta tcttcattga tttcaaaatc ttcattgtct tcatttgctt ctttgctgtc
     7741 aattctttca tctgaatcta tgattctttt ataatattcc ttgcttgcat ttcctaatac
     7801 ttttttgata ttttcagcag taacaatacc tatatccatt ttatacttat ttttgatata
     7861 ttccttaata ttaatatcga tatcatctcc tgcaatcctt agagaatcgc tttcaacaat
     7921 tcctcccagt gatattactg caatctcggt tgtaccccct cctatatcaa caatcatatt
     7981 gcctatcggc tggtctacat taagcccggc gccaattgca gctgccatag gttcttcaac
     8041 aagttttacc ttccttgcac cggcatcata tgctacttct tccactgctc ttctttcaat
     8101 tgaagtaact cccacgggaa cacttattac tatatttgat ttaataaatc tttttttgtt
     8161 aagagccttt gttataaagt attttatcat tgcccttgtg atttcaaagt ctgcaattac
     8221 tccgttttgc aacggtctta tcgcccttat ggtctttgga gttctctcaa gcattgcttt
     8281 tgcatcaaaa ccgactgcac atacctcata tgacgtcgtg tttattgcaa ccactgatgg
     8341 ttcatttaaa gtaattcctt tattgtttac atatactaat gtgtttgatg ttcctaaatc
     8401 tattcctatt ttctgagtaa aaaaatcaaa tagtcccatt tatgtatcct ttcattttaa
     8461 attaagcctt cttccttaaa actgaaatat acgccgtcac cgattattat atgatctaaa
     8521 actctgattc ccaaaatttt gccgctttca tccaacctgt ttgttaaaac aatatcctca
     8581 tggctgggct cgcaatctcc gctggggtga ttgtgaacta atattattga agaagccaaa
     8641 tttacgactg cttcataaaa tacttcccga ggatggacaa ttgaactgtt taagcttccc
     8701 actgatattt cagaaacctt ttttacgttg tttttggtgt ctaaaagcat tattttaaaa
     8761 tactcttttt gataatatct catttcttcc atcatcatca cagctgcatc atcaggagaa
     8821 gaaatcttta atttttcaat ttttaatgtg ctcattcttt tgcttatttc tgcaagagcc
     8881 ataagctttg cagccttagc gtcactgatt cccttgtagg atttaaactt ttccagactt
     8941 ccttctgcaa tatttcgaag ccctttgccg ccttccttta gaatcctctg agacagcata
     9001 acaacatttt cttccctcgt tcctgtcctt aaaattactg caataagctc tgagttagac
     9061 aatgcaccgg ctccatattt taatagtttc tcctgaggcc tgtcatctgc aggcaatgat
     9121 tttatggtat aattagtatt gtccatttta tcccccacta tgcaatattt gattacagca
     9181 agtcgtaaga aaaatgtttt gaaagcaaat tattcagttt tgcaataggc agccctttta
     9241 cattattgta gcatccatgt atttctttaa ccaataattc tccgtatcct tgaataccgt
     9301 atgctcctgc tttatccaaa tactcatatg ttttcaagta tcttttaatt aatccttctg
     9361 taagctcatt aaacacaact tgagttttct cataatccac tatctttttt gagctttctg
     9421 tatcaattat acaaatacct gtaaacacat agtgagtttt tcctgaaagc agactaagca
     9481 ttttataagc ttctgcttca tcttttggct tgcccattat ttcgtctaaa tatacgattg
     9541 tatctgcagc tataatcagt gcattttctt tatattttaa atagacattt aatgcctttt
     9601 caaaagctac cttcataacc gttgtttggg ggtaatcaaa agaatttacc ttttctacga
     9661 tatcaggaat ataagttatt atgtctacat atttcttcat catttcaatt cttctcggag
     9721 aatttgatgc caatactatt ttcttcatat taatccttat agttttctga atattattat
     9781 tcctgcaatc agaccgatta ttcctgcaac agtcagtctg gcgctgaatc cgaatgtaat
     9841 agtgaaaatg ttcaaatcca aagttgcagg accaaaacca attccttcgc cataatttag
     9901 tatgggaagc atatttgaaa atgccttacc taaaattgtt cctataatta cagatgtaag
     9961 tatgagtaat atcaaataaa aagaattgcc ttttctcatt acacgcctct cctccaagtt
    10021 ttaacagtgg tttccaagtt gtttgttaca aaaattgctg tatatcctac aaagcatccg
    10081 gtaaaaatac ttaccaatga caatatgggc aaataataat acaagttcac ggtagtaaac
    10141 aatattgcag ccaccgttac ctgagttgta ttatgtgcca ttgctcccaa aacgctaatc
    10201 ccgattaaac taaaaatatt gcttaaatac ttacatgcca atgacattac aattgtgctt
    10261 acgataccgc tcgcaatact gtaaataaat gtaatgggat ttgtagcaac aagcattaaa
    10321 agaatacttc ttattataac aacaagaaga gcatccttaa aaccaaatac aactactgtc
    10381 gatagtatta caatatttga taaacctaac tttgctccgg gagctatgta aggtaatggc
    10441 atcatacctt ctattatgct aagtatcagc gctgccgcag tcaaaagcga taaaaaaata
    10501 tacctatgga ttttattcat aatattctct ttctaatcag ctatgaaatc aatttcactt
    10561 tcgtcatttt cgtctacaat ttttataaac attctatttg gaatacatac aatcatctcg
    10621 ccgttttttg atattttgcc ctgatatatg caaattttat cgggacagct tgcttcgtgt
    10681 atccaaattt gtccattttc aatatgcatt atattataac ctgtttcaga ttctatgtta
    10741 actacatcct tataatcgtc ggttaaaaca tattctccca ccaatttatt gttagaataa
    10801 atatatgctg ttttgctatt gctgatcacg gcatcccgac cgccatacaa aataaa
//
LOCUS       DAOSOW010000009        10698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_6162_length_10698_cov_4.290520, whole genome shotgun sequence.
ACCESSION   DAOSOW010000009 DAOSOW010000000
VERSION     DAOSOW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6162_length_10698_cov_4.290520"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLD67_00640"
     CDS             <1..321
                     /locus_tag="PLD67_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOT21065.1"
                     /translation="QNDMITFKIAKNEDYWFHAKNMPGSHVIIRTKGDELTDEEFIEA
                     AKIAAYYSKGKNSSTVEVDYTKKSNIKKPPNAKPGFVIYDTNYSMLVKPNIEGIKRDS
                     GDVS"
     gene            complement(366..896)
                     /gene="pyrR"
                     /locus_tag="PLD67_00645"
     CDS             complement(366..896)
                     /gene="pyrR"
                     /locus_tag="PLD67_00645"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013298022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOT21066.1"
                     /translation="MKIKALIMDEKAIERATFRIAHEIIERNKGVENIVLIGIKTRGW
                     PLSIRLAKKIEEIEGSKVPVFPLDITYYRDDVEKDDRAPLSVKSFDFDIKNKTVVLVD
                     DVLYTGRTVRAALDAIVDQGRPNNVELAVLIDRGHRELPIRADFIGKNVPTSRSERIS
                     VRLEETDKINQVVITE"
     gene            complement(953..1870)
                     /locus_tag="PLD67_00650"
     CDS             complement(953..1870)
                     /locus_tag="PLD67_00650"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT21067.1"
                     /translation="MEISDSEKEIILVSDEENKRIDVFISEKSGESRNFVQKLISDGN
                     ITVNEREVKANYKVKINDTIKIIIPPPEILDVKAENIDLDIVYEDNDLAVINKPQGMV
                     VHPAPGHYSGTMVNGLMYHLKNLSTINGVLRPGIVHRLDKNTSGLILVAKNDRSHNFL
                     AKCLKEHTINRIYYALVKGNIKKDEGEINAPLGRSEKDRKKYTVTNKNSKEAITNYWV
                     VERFGKYTLLKLKLKTGRTHQIRVHMKHIGHPVVGDDVYGNVNEFGLEGQLLHSKILG
                     FIHPTTGKYMEFESELPDYFVKVLNKIKS"
     gene            complement(1986..2450)
                     /gene="lspA"
                     /locus_tag="PLD67_00655"
     CDS             complement(1986..2450)
                     /gene="lspA"
                     /locus_tag="PLD67_00655"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOT21068.1"
                     /translation="MSIIAVFIIIFSVVFDQLTKYWATEVLKNGESIKIIGNFLRFTY
                     AENRGAAFSILQNQRVFFLIITIIMLIILGIVYFTNRNLSKLSRLSMAMIAGGAIGNF
                     IDRYRMGYVIDFIDVRFGTFYNFPVFNIADSFVVCGTILMVILILFNKFETS"
     gene            complement(2462..3244)
                     /locus_tag="PLD67_00660"
     CDS             complement(2462..3244)
                     /locus_tag="PLD67_00660"
                     /inference="COORDINATES: protein motif:HMM:NF037631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HOT21069.1"
                     /translation="MKSLEKYYDFIRDDEIKNTVKKIQDKASYVNKNYIAAITEFINP
                     YVVKISIPVIKSYDIKFEIFPSYENCERKVFILYPQYSEIDENEFISGIRITNKSKFK
                     KLSHKDYLGVLMSLGIERSKLGDIYVCDDYGDVVVHSDIADYIIYNLDKIGHNKIEAE
                     KINLSQVNFKEQEHEILNIITSSMRIDNIVKHLTKLSREKASFIIKSGDVKINHIPEY
                     KVSTELKEEDILSIRRAGRFKIYKIIGTTKSGKLKIQIKHYI"
     gene            complement(3544..3975)
                     /locus_tag="PLD67_00665"
     CDS             complement(3544..3975)
                     /locus_tag="PLD67_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HOT21070.1"
                     /translation="MMEKVKDFIGITDLEEDYEEEDLVSEGTERNKNERMGTNTKRNN
                     VIKVHSNTDMKVFICEPEKYEDCTKAVDEIKNRKVVVLNIEGMELEDQKQVFEFIKGA
                     IYALEASIQKVSNGIFVLAPNNVQIDGRLSDRYERNYYYGK"
     gene            complement(3982..4686)
                     /locus_tag="PLD67_00670"
     CDS             complement(3982..4686)
                     /locus_tag="PLD67_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOT21071.1"
                     /translation="MSIRENIDDIVKRIEDTCKKVGRNPNDITVIAVSKTVESERARE
                     AFEAGINNLGENRVQELVKKYDELKDLDIKWHMIGHLQKNKVKYIIDKTVLIHSVESL
                     SLAEEINKRAEKNNLIANVLVELNIGEEDSKFGIKEESVYDFILSLEKFENLRVVGLM
                     TVAPFCENPEDVRWVFKKMKYIYDKISTMNLRNTEMKYLSMGMTNDFEIAIEEGSNII
                     RIGTAIFGARKYKEEE"
     gene            complement(4696..5922)
                     /locus_tag="PLD67_00675"
     CDS             complement(4696..5922)
                     /locus_tag="PLD67_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOT21072.1"
                     /translation="MARKNFGLKNFFLSFALVVIAIYVFQNIKSPADFMIAENGILED
                     VIKAEGIVVKDEKVYSATLDGNVLYYYEEGTKIKQGQLIADINTDADSVEVNRQIAEI
                     QAAINKLENSSQVLTKENFTAYQNELQISILNNDLQNMYGIVEQMTNNDFYGSKYEDF
                     SISQLIEMKNSLANSISTHKVPYYSDRAGIISYKTDGLEGIYRFENVMDMTPSSTLRR
                     EYSLKDKRESVQKDEAFYKIIRNFEYYIIVTINNEKARLFEENKYIKVRILSDGEQHI
                     VWGYIEKINYGSEESVLILYFDDYFYKVYDKRYLNIELITAAYEGLKIKNESLIEVNG
                     IKGVYVQDASNIIKFFPVQILGSNEEYSVVFPGDYIGINERNIITIGEKRYPTIKIFD
                     KVILEPDKVYEGQIAD"
     gene            complement(6156..7283)
                     /locus_tag="PLD67_00680"
     CDS             complement(6156..7283)
                     /locus_tag="PLD67_00680"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOT21073.1"
                     /translation="MERDKYFEEYYEKKRNNISFITLKKGAAVNYKDKTYAAKKELPV
                     PVRIEKLLEDINKQNEMDGITLNNIIDGIIYILGTDRNFEYIQDYKEMFKELNFELIP
                     YIIYCINNDIKTEDGVVYGRALINSEENEKTCFIYASCLEKMGMEYHEKGNDSSRYFL
                     EEAHFYYEKCLDYNDKFSLAYYKLGYYYKRKQQYVKAELIWQKHQELDDNDLRIEEIR
                     NELIQLKPFVDYEKGYNLVLKERPDEALDLLLPLVKDFGGWWNLLFFIGLAYRSKREY
                     DIAETYFENVLKIDSTQKETLNELGLCKICTGKYVEAAELFSKLLSLDPGNCEVFCNR
                     AVAYLYNNQLERAKEDIQTALKINPEDAVALGIKEELEKKY"
     gene            complement(7270..8982)
                     /locus_tag="PLD67_00685"
     CDS             complement(7270..8982)
                     /locus_tag="PLD67_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HOT21074.1"
                     /translation="MKILLVTLDSKFIHANLAVRYLKKYCRGFDIVIKEFTINQKIEY
                     ILDEILAEDADLICFSCYIWNIDYIKDISYIIKESNNKAKILYGGPEASFEIKELMEN
                     NPFIDYVIFGEGEETFKEFLEEIQKTNPNLHKIRGLAYKENNDVIINEGREPIDNLDI
                     INYPYEVDDKFANKIIYYESSRGCPFSCSFCMSSIDKKMRFFSMDRVKKDLKMLLNTK
                     ARQIKFVDRTFNADFRRSMEIMQYIVENNRNNMTIHFEITADIINDEFLSFIAKMPVN
                     MFQFEIGVQSLNEDTLREINRHMDKDKLCRVIKEIGKNKNVHMHLDLIAGLPYEDYES
                     FKRSFDGIYNLDAEKIQLGFLKVLKGTKIYEDREKHQIKYGLKAPYEVICTKYISLKE
                     LLKLKNIEELLDKYYNEKYFSKSLKYITCYIFPDSAFEFYSSFSDYWQKYNLYNYAHS
                     RKELYKILYEYIVHMKRKNDEFVQALKFDYIFNNQYEELPDYLNRETEDKFKSIKRQL
                     ANDIEFKNKYFPSMKGKIINDFRIAEIGGKIVLFVYRDRENIFNRCDTYVVNNIEEIV
                     YGKR"
     gene            complement(8979..9833)
                     /locus_tag="PLD67_00690"
     CDS             complement(8979..9833)
                     /locus_tag="PLD67_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21075.1"
                     /translation="MQYKINKMLNFCFDDIQKIDFQLFLITVKCLIPYDDKIGCLFDK
                     DRYKKEIELFSSYMNGNDETIDYWLQNKEASNLEDNLLEYKIIPLALSNTVWENLTDE
                     LIKVVLFYTINKNTILNALLISSAVFDFMTGSDIDSIEAAARERLINFSVKEFLIKNQ
                     TNADKNYIIDFEKERIKMIAKPILFDNNFINGFKVLNYIFEKSPEHLTENNSDIIDSF
                     SVYLYKLRKGLISPEKLIIPDKIPEIKECLKYSTFSHPLLGRCKVIKRTEKEAIVRNK
                     LGLMRIRI"
     gene            complement(10012..10659)
                     /locus_tag="PLD67_00695"
     CDS             complement(10012..10659)
                     /locus_tag="PLD67_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOT21076.1"
                     /translation="MKRVNYLTFLIFLCIFSYFMMINTSMTAIAEDNQNYYKFSQDKI
                     IKSIIHDYRDSFIREKELIRYTEELTGLDYEHCRFLIKESKEKYIDPFIVLGLIKRES
                     DFNPKAKGVGGELGLGQLMENTAQIIAMNLGYSYNNEMLLDPVFNLKLTIAQLSYLNK
                     VYDNDMHKTLTAYNRGQQGLKNYLTTCDGRGVSDYSTKVLQFASIYKEEFENLKY"
BASE COUNT         3155 a         2058 c         1374 g         4111 t
ORIGIN      
        1 caaaatgata tgataacatt taaaatagct aaaaatgaag attattggtt ccatgccaaa
       61 aatatgcccg gctcccatgt tatcataaga acgaaaggcg atgaactaac ggatgaagaa
      121 ttcattgaag cagctaaaat tgcagcatat tacagcaagg ggaaaaattc aagcacggtt
      181 gaagtcgact acacaaaaaa atcaaatata aaaaagccac ctaatgcaaa gcccggcttt
      241 gtaatttacg acacaaatta ttcaatgctt gttaagccga atatagaagg gataaaaagg
      301 gacagtgggg acgtatcttg actgtcacag gtatgggaca gcaaggtacg tccccactgt
      361 cccgcttatt cggttataac cacctggtta attttatctg tttcttcaag ccggacgctt
      421 attctttccg acctggaagt aggtacgttt ttaccgatga agtctgcacg aattggaagt
      481 tccctgtgac ctctgtcaat taatactgct aattccacgt tattaggtct tccctggtcc
      541 acaatggcat caagggcggc tcttactgtc ctgcctgtgt ataaaacgtc gtcaaccaat
      601 accactgttt tattcttaat gtcaaagtca aaacttttta ctgacaaggg agccctgtca
      661 tccttttcga catcatctct ataataagta atatccaaag gaaaaacagg cactttggag
      721 ccttcaattt cttcaatttt tttcgccaat ctgatagata agggccatcc tcttgttttt
      781 attccgatta gtactatatt ttcaactccc ttatttctct cgataatttc atgtgcaatc
      841 ctgaatgttg ctctttcaat cgctttttca tccattatta atgcttttat tttcataaat
      901 tccctcccaa tatgagatct ttcgctatcg ctcaggctga catgcaatgc ccttaagatt
      961 ttattttatt caatacttta acaaaataat ccggtaattc actttcaaat tccatatact
     1021 tgccggttgt gggatgaata aagcccaaaa ttttcgaatg caggagctgt ccttccaaac
     1081 caaattcatt tacatttcca tatacatcat caccaacgac gggatgtcct atatgcttca
     1141 tgtgaaccct tatctgatga gtcctgccgg tttttaattt cagtttcaat aaagtatatt
     1201 taccaaacct ttccactacc caataatttg taattgcttc cttgctgttc ttatttgtta
     1261 cggtatactt tttcctgtct ttttcgcttc tccccaaggg tgcgtttatt tctccttcat
     1321 ctttcttgat atttccttta accaatgcat aataaatcct gttaattgtg tgctccttta
     1381 aacacttggc taaaaagttg tgtgatctgt catttttagc aaccaagatc aaacctgaag
     1441 tatttttgtc taatctgtga acaataccgg ggcgtaaaac cccgttaatg gttgacaaat
     1501 ttttcagatg atacataagt ccgtttacca tagtgcctga ataatgcccc ggggcaggat
     1561 gaacaaccat tccctgaggt ttatttataa ccgctaaatc gttatcttca taaacaatat
     1621 ccaagtctat gttttctgcc ttcacatcca aaatttcagg aggcggtatt ataattttta
     1681 tagtatcgtt tattttaact ttataattag ctttaacttc tctttcattt acggttatat
     1741 ttccatcgct gataagcttt tggacaaagt ttcgcgattc gcctgatttt tcagaaatga
     1801 acacgtcaat tcttttattt tcttcgtctg aaacaaggat aatttctttt tcactgtctg
     1861 aaatttccat gtcttcaaaa tcatcatcat cggaattgtc aaaatcatct atttctttaa
     1921 tatcgtctaa attttctata tctttttccg ttgtactatt taaattattt aactttttca
     1981 ttttctcaac tcgtttcaaa cttgttaaat agaattaaaa ttaccataag tattgttccg
     2041 catacaacaa agctgtcagc tatgttaaat accggaaaat tataaaaagt tccaaatctt
     2101 acatcaataa aatctattac atatcccatg cgatatctgt caataaaatt gcctattgct
     2161 cctcctgcaa tcatagccat ggataatctg gacaactttg aaagatttct gtttgtaaaa
     2221 taaactattc ctaatataat aagcattata atagtaataa tcaaaaagaa taccctttga
     2281 ttctgcaata tgctgaatgc agcacctctg ttttccgcat aagtgaaacg caaaaaattt
     2341 cctataattt ttattgattc accgtttttt aaaacttctg ttgcccaata ctttgttaat
     2401 tggtcaaaaa caactgaaaa tataattata aatactgcta taatactcat tacttctccc
     2461 gttaaatata atgcttaatt tgaattttta gctttccgct ttttgttgta ccgattattt
     2521 tatatatttt aaaccgtcct gcccttctga ttgacaagat gtcctcctcc ttcaattctg
     2581 tgcttacctt gtattcagga atatgattga ttttaacatc tcccgatttt attatgaaag
     2641 aggccttttc ccttgataat tttgtaaggt gctttacgat attgtcaatg cgcatggagg
     2701 aggtaataat attcaagatt tcatgttcct gttctttaaa attgacttgg cttaagttaa
     2761 ttttttctgc ttctatttta ttatgaccta ttttatccaa attgtaaatt atataatcag
     2821 caatatccga gtgaaccact acatccccgt aatcatcaca gacatagata tccccgagct
     2881 tgcttctttc aatacctagt gacatcaata ctcctaagta atccttatgg cttaattttt
     2941 tgaatttaga cttattagta atacgaattc ccgaaataaa ttcattttca tctatttcag
     3001 aatattgcgg gtataaaatg aaaactttcc tctcacaatt ttcatatgag gggaaaattt
     3061 caaattttat atcataactt tttattaccg gaatactgat ttttacaaca tatggattta
     3121 taaattcggt tattgcggca atatagtttt tattaacata cgatgcttta tcttgtatct
     3181 tttttaccgt gtttttgatt tcatcatccc tgataaaatc ataatatttt tctaaacttt
     3241 tcatttttct cccggtagta ttgcagccat ctatatggcg tatatcaatc tttgtaatat
     3301 gtttaataat agaagagcca atattggaga aaaatcaaac attcccatgc ctaatcccag
     3361 tttatctaaa attgccctgc aaggagccag tacaggctcg gtcatttgat agataaagta
     3421 ggatattctt gaagtttgta gggttgggaa aaatgataat atcactctga ccagtattaa
     3481 ataataaatt attcttaata gtgatgctag agccaactgt ataatataaa acataaatgc
     3541 tccttattta ccgtaataat aattcctttc atatctgtcg cttaatcttc cgtcaatttg
     3601 cacattgttc ggagctagta cgaatatacc gtttgatact ttttgaatgc ttgcttccaa
     3661 tgcataaatt gcacctttga taaattcaaa tacttgcttt tggtcctcaa gttccattcc
     3721 ttcaatatta agaactacaa cttttctgtt tttaatttca tcaacagctt ttgtgcaatc
     3781 ctcatatttt tccggctcac aaataaagac tttcatatca gtgttggagt gaaccttaat
     3841 cacattgttt ctttttgtgt tggttcccat tctttcattt ttgtttcttt ctgttccttc
     3901 agaaacaaga tcctcctcct catagtcttc ttccaagtct gtgatgccaa tgaagtcctt
     3961 cactttttcc atcatactca tttactcttc ctccttatat tttctggctc caaatatagc
     4021 tgttccgata cgaataatgt tagagccctc ctcgatagca atttcaaagt catttgtcat
     4081 gcccatggac aaatatttca tttctgtatt tctcaaattc atagttgaaa ttttatcata
     4141 tatatatttc atttttttga aaacccatct tacatcctca gggttttcgc aaaacggggc
     4201 aaccgtcatc aatccgacta ctcttaaatt ttcaaacttc tccaaactaa gtataaaatc
     4261 ataaacactt tcttctttta tgccaaactt actgtcttct tctccaatat taagttcaac
     4321 aagtacattt gcaattaaat tgtttttttc tgctctttta tttatttctt ccgccagact
     4381 taaactttca acagaatgaa taagtactgt tttatctatt atgtatttta ctttattctt
     4441 ttgcaaatga cctatcatgt gccatttaat gtccaaatct ttaagctcgt cgtacttttt
     4501 taccagttcc tgaaccctgt tttctccaag gttattaatt cctgcctcaa aagcttctct
     4561 tgctctttct gattctaccg tctttgatac agctatcact gttatatcat tcgggtttct
     4621 tccgactttt ttacaggtat cttcaattct ctttacaata tcgtcaatat tttctctgat
     4681 gctcaacttt gcctcctaat ctgcaatttg accttcatac accttatcag gttcgagtat
     4741 tactttatcg aaaatcttaa ttgtaggata tcttttttct ccgatagtta ttatattcct
     4801 ttcatttata cctatatagt cacccggaaa tactaccgag tattcctcat tgctccctaa
     4861 tatttgaacg ggaaaaaatt tgattatatt actggcatct tgtacataca cacctttgat
     4921 accgttaact tctatcagtg attcgttttt tattttcagt ccttcatatg ctgccgtaat
     4981 cagctctata ttaaggtatc tcttatcata aaccttgtag aaatagtcat caaaatacaa
     5041 tattaataca gattcttcgc tgccgtaatt tattttttct atataacccc agactatgtg
     5101 ctgttcccca tctgaaagta ttctaacctt tatgtattta ttttcttcaa acagtcttgc
     5161 tttttcatta tttatagtta caattatata atattcaaaa ttccttatta ttttatagaa
     5221 agcttcatcc ttttgaacag actctctctt atccttcaat gaatactcac gtcttagggt
     5281 gctgcttggg gtcatgtcca taacattttc aaatctataa ataccttcta atccatcggt
     5341 tttatatgat attattcccg ccctgtctga ataatatgga accttatgag tagaaatact
     5401 gtttgccaag ctatttttca tttcaatcag ctggctgatt gaaaaatctt catatttgct
     5461 gccgtaaaaa tcattattgg tcatttgttc gactataccg tacatattct gaaggtcatt
     5521 gttcagtata ctgatttgta attcgttttg ataagcagtg aaattttctt tagttaaaac
     5581 ttgactgctg ttttccaatt tatttatggc agcttgtatt tcggcaattt gtctgtttac
     5641 ttcaacggaa tccgcatctg tatttatgtc tgctataagc tgtccttgtt ttatttttgt
     5701 accttcctca taataataca aaacgttgcc atcaagggtt gcactgtaca ctttttcatc
     5761 cttaacaaca attccttcag ccttaataac atcctctaat attccgtttt ctgctatcat
     5821 aaagtctgca ggagatttta tattttgaaa tacataaatg gctataacaa ctaatgcaaa
     5881 agaaagaaag aaatttttta aaccaaaatt ttttcttgcc actattaatc tccaagttct
     5941 atattaagtt ctcttatgta tcgagccctc atgaaattct tcaggtttcg ccttcagaat
     6001 gacactcgct gtataattta tcatcaattg tacaaccatt ataacataat cttcatatct
     6061 tgtgcaagct atattaagag attttcgaga aatattgcaa taattattaa aattaatcaa
     6121 tatatatgac acatttttta cttgctaatc ttatttcaat actttttttc caattcttcc
     6181 ttaataccga gtgccacagc atcttctggg ttgattttta acgctgtttg aatatcttct
     6241 tttgctcttt caagttgatt gttatacaaa taagctactg ccctgttgca aaatacttca
     6301 cagttgcccg ggtccaggct taacaatttt gagaacaatt cagctgcttc gacatatttg
     6361 cctgtgcata ttttgcacaa gcccaattca tttaatgttt ccttttgagt gctgtcaatt
     6421 tttaatacat tctcaaagta tgtttctgca atatcgtatt ctctcttcga cctgtatgca
     6481 agacctatga aaaacaacaa attccaccat ccgccaaaat cctttaccaa aggcaataat
     6541 aagtccaagg cttcgtctgg tctttctttt aatacaagat tgtagccctt ttcataatca
     6601 acaaatggtt ttaattgaat taattcgttt cttatttctt ctattcttaa atcattatca
     6661 tccaattcct gatgtttttg ccaaatgagc tcggctttta cgtattgctg cttcctttta
     6721 taataataac ctaatttata gtatgccaat gaaaacttgt cattatagtc aaggcatttt
     6781 tcataataga aatgagcttc ttccaaaaaa tatcggcttg aatcatttcc tttttcatga
     6841 tattccatcc ccatcttttc caaacatgaa gcataaataa agcaggtttt ttcattttct
     6901 tcactgttaa tcaaagctct tccgtatacc acaccgtctt ctgttttaat gtcattgttt
     6961 atgcaatata taatataagg aattaattcg aaattgagtt ctttaaacat ttccttataa
     7021 tcttgtatat attcgaagtt tctgtcagtt cctaatatat atattatgcc gtcaataata
     7081 ttgttcagtg ttattccgtc catttcattt tgtttgttta tatcctccaa aagcttttca
     7141 attcttacag gtacaggcag ctcttttttt gcagcatatg ttttatcctt atagttaacg
     7201 gctgcacctt tttttaaggt tataaatgaa atattatttc tttttttttc ataatactct
     7261 tcaaaatatt tatctctttc cataaacaat ctcctctatg ttattaacaa cataagtatc
     7321 acatctgttg aaaatattct ccctgtccct atatacaaat aatactattt tccctccgat
     7381 ttctgcaatt ctgaaatcat ttataatttt ccctttcatt gaagggaaat atttattttt
     7441 gaattcaatg tcatttgcca gctgccgctt tatgctttta aatttatctt cagtctccct
     7501 gtttaaataa tcaggcagct cttcatattg gttgttgaat atataatcaa attttaatgc
     7561 ttgaacaaat tcatcattct ttcttttcat atgtactata tactcataca aaattttgta
     7621 aagctctttc ctgctgtgag cataattata taaattatat ttctgccagt aatcactgaa
     7681 agagctgtaa aattcaaaag ctgaatcggg aaatatatag catgttatat attttaagga
     7741 tttgctgaag tatttttcat tataatattt atccagcaat tcttcaatat tttttaattt
     7801 taataattcc ttcaaactta tatattttgt gcatatcact tcatatggag ccttcaagcc
     7861 gtatttgatt tggtgctttt ctctgtcttc gtatattttg gttcctttca gcacctttaa
     7921 aaaaccaagc tgaattttct ctgcatcaag attatatata ccgtcaaatg accttttaaa
     7981 actttcataa tcttcataag gaagccctgc aatcaagtca aggtgcatat gtacattttt
     8041 gttttttcct atttccttaa taacgcggca taatttatcc ttgtccatat gcctgttgat
     8101 ttcacgcaga gtatcttcat tcaaggattg aacaccaatt tcaaactgaa acatattaac
     8161 cggcattttc gctataaagc ttaaaaactc atcatttatt atgtcggcag ttatttcaaa
     8221 atgtattgtc atattatttc tgttattttc tacaatatac tgcattatct ccatggagcg
     8281 cctgaaatct gcattgaatg ttctgtccac aaacttaatc tgccttgcct ttgtatttaa
     8341 aagcatcttt aaatcttttt taactctatc catcgaaaaa aatctcattt ttttatcgat
     8401 ggatgacatg cagaagctgc agctaaaagg acatcccctt gaagattcat agtatattat
     8461 tttatttgca aatttatcat caacctcata ggggtaatta attatgtcga gattatctat
     8521 cggctctctg ccttcgttta tgattacatc attgttttct ttatatgcca gacccctgat
     8581 tttatgaaga ttgggatttg ttttttgaat ttcttcaaga aattctttaa atgtttcttc
     8641 cccttccccg aatattacat aatcgatgaa ggggttgttt tccatcaatt cttttatttc
     8701 aaaagaagcc tcgggacccc cgtacaatat ctttgcttta ttatttgatt cctttattat
     8761 gtatgaaata tcctttatat aatcaatatt ccatatatag caggaaaaac aaattaaatc
     8821 tgcatcttca gccaaaattt catccagtat gtattcgatt ttttgattta ttgtaaattc
     8881 ttttattaca atatcaaaac ccctgcaata cttttttaaa tatcgcactg ctaaattagc
     8941 atgaataaat tttgaatcta aggttaccaa caatattttc atattctaat cctcatcagc
     9001 cccaatttat tcctaacaat tgcttctttt tcggttcttt ttataacctt gcatctccct
     9061 aacaggggat ggctgaatgt cgaatacttt aagcattcct ttatttccgg aattttatcc
     9121 ggtattatta atttttcagg acttattaaa cccttcctga gtttgtacaa atatacagaa
     9181 aagctgtcta ttatatccga gttattttcg gttaaatgtt ccgggctctt ttcaaatatg
     9241 taatttaaga ccttgaatcc gttgataaaa ttattatcga acagtatagg cttagcaatc
     9301 atttttattc tttctttttc aaaatcgatt atataatttt tatctgcatt tgtttgattt
     9361 tttattaaaa attcttttac cgaaaaattt ataagccttt cccttgctgc tgcctcaata
     9421 ctgtcaatat cagagcctgt cataaaatca aaaactgcag atgaaataag caaagcattc
     9481 aatatggtat ttttatttat tgtgtaaaat aaaaccactt ttataagttc atccgttaaa
     9541 ttttcccaca cagtgtttga caaggcaagt ggaatgattt tatactctaa aagattgtcc
     9601 tccaaatttg acgcctcttt attttgcagc caataatcaa ttgtttcatc attaccattc
     9661 atataagagg aaaataattc aatttccttt ttatatctat ctttatcaaa aaggcatccg
     9721 attttatcat catagggtat gagacatttt accgtaatta aaaagagctg aaagtcaatt
     9781 ttttgtatgt cgtcgaaaca gaaatttagc atcttgttta ttttatattg catatattct
     9841 cctaaatcgc attggaatgc cgcaaattta tggcattcct tgcgtttgac cccttatccc
     9901 tttatgtttt gctattacat cagcaatgga gtcaggtcat aaaaaagaat tcgcagtatt
     9961 aactgcgatt ccattataca atcaattatt gaaattatca aatgctatta atcaatactt
    10021 taaattttca aattcttctt tataaatgga tgcaaattgt aaaaccttag tagagtagtc
    10081 actaacacct ctcccatcac atgttgtcaa atagtttttg agtccttgct gacctctgtt
    10141 gtaagcagtg agagttttat gcatgtcatt atcgtaaact ttattaagat atgaaagctg
    10201 tgcaattgtt agttttaaat taaataccgg gtctaaaagc atttcattat tatatgaata
    10261 tcctaaattc atagcaataa tttgggcagt attctccatc agctgaccca aacccaattc
    10321 tcctccgaca ccctttgcct tgggattaaa atcactttct ctttttatta aacccaatac
    10381 aataaagggg tctatgtatt tttctttgct ttctttaata agaaatctac agtgctcgta
    10441 atctaatccc gtcagttctt ctgtataccg gatcaattct ttttcgcgaa taaatgaatc
    10501 cctgtaatca tgaataattg attttattat tttatcctgt gaaaatttgt aataattttg
    10561 attatcctct gctattgcag tcatgcttgt atttatcatc ataaagtaag aaaatataca
    10621 tagaaatatt aaaaaagtca aataatttac ccttttcatt tcgtacctcc ttgtccaata
    10681 ttaattataa tgtaaatt
//
LOCUS       DAOSOW010000010        10478 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_6350_length_10478_cov_3.993092, whole genome shotgun sequence.
ACCESSION   DAOSOW010000010 DAOSOW010000000
VERSION     DAOSOW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 10478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10478
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6350_length_10478_cov_3.993092"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            544..771
                     /locus_tag="PLD67_00700"
     CDS             544..771
                     /locus_tag="PLD67_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HOT21077.1"
                     /translation="MATNNSGSNNIVVPEAKQALNQMKTEIANELGLTNYEQIDKGNL
                     TARQNGYVGGYMTKRLVEMAQRQMSGGGQFK"
     gene            complement(882..1907)
                     /locus_tag="PLD67_00705"
     CDS             complement(882..1907)
                     /locus_tag="PLD67_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009065316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOT21078.1"
                     /translation="MNISEYKGVFVFAEQRDRTVQKVAYELIGKAKELAKDLNTTVTA
                     VLLGYDMIEEAKKLCYAGADKVIYADNELLDEYMTEPYTFTLHKIITEKKPEIVLYGA
                     SAIGRDVAPRVSARLKTGLTADCTGLAITEDDTNKGQLNLAMTRPAFGGNLMATIIIG
                     KHRPQMATVRPGVMQKAKYSENNPVNIEQFMIDIPADKKNVEILDITKIIQQRMNIED
                     AKVLVSGGRGMHGPENYQMLEELAGLLGGTISASRAAVDAGWVPKDRQVGQTGKTVRP
                     NLYIACGISGAIQHLAGMEESDFIIAINKDETAPIFEIADVGIVGDLFKIVPLLIEEL
                     KKSKQMA"
     gene            complement(1920..2702)
                     /locus_tag="PLD67_00710"
     CDS             complement(1920..2702)
                     /locus_tag="PLD67_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009531083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOT21079.1"
                     /translation="MNIVVCIKQVPDTTEVRIDPKTNTLIREGVPSIINPDDKAGLEA
                     ALRLKDEYNAHVTVLTMGIPNAEDALREALAMGADEAILITDRAFGGADTLATSTTLA
                     AAIKNLDYDLIITGRQAIDGDTAQVGPQIAEHLGLVNISYAEDIKIDGDKIIVKRQYE
                     DRYHLLKTKLPCLITALAELNKPRYMTPGGIFDAYREKRIKIWNLKDVDISQECVGLK
                     GSPTKVVKSFTKGAKSAGKVFSLEPQESAELIVEKMKEKFII"
     gene            complement(2716..3855)
                     /locus_tag="PLD67_00715"
     CDS             complement(2716..3855)
                     /locus_tag="PLD67_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOT21080.1"
                     /translation="MNFQLSKEHEMARQLFRNFAVEEVEPLAQEIDEEERFPVETVEK
                     MSKYGFMGIPFPKEYGGQGCDYLTYAMAVEELSKVCATTGVIVSAHTSLCGGPLYEFG
                     TEEQKKKYLTKVASGEWVGAFGLTEPGAGTDAAGQQTKAVLDGDNYILNGTKIFITNG
                     SYADCYIIFAMTDKSQGTKGISAFIVEKNFPGFSIGKKELKMGIRGSATCELVFEDCI
                     VPKENLLGKEGQGFKIAMKTLDSGRIGIAAQALGIAQGAIDESAKYVKERKQFGRAIA
                     KFQNTQFRLADMQTKVDAARLLVYRAACAKDAGQAYSVYAAMAKLFASETAMEVTTKA
                     VQLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVMKMVVSANMLK"
     gene            complement(3953..4726)
                     /locus_tag="PLD67_00720"
     CDS             complement(3953..4726)
                     /locus_tag="PLD67_00720"
                     /EC_number="4.2.1.150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain-enoyl-CoA hydratase"
                     /protein_id="HOT21081.1"
                     /translation="MEFKNLLFEKNEGICTIKINSPQTLNALNEEVLEELDSAFDLAG
                     KDEEVSAIILTGEGKAFVAGADIAYMNNLNVEEAKKFAEYGSKIFRKIELLEKVVIAA
                     VNGYALGGGCELCMACDIRIASEKAKFGQPEVGLGITPGFSGTQRLPRLVGLGRAKEL
                     ILTGGHIDAEEAYRIGLVNKVTNKDSLMEEAFTLAKKIISNSKVAMKYAKEAMNRGIE
                     TDIETGIAIEANLFSLCFADEDQKEGMTAFMEKRTAKFK"
     gene            complement(4930..5574)
                     /locus_tag="PLD67_00725"
     CDS             complement(4930..5574)
                     /locus_tag="PLD67_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOT21082.1"
                     /translation="MIDTVIFDFDGTLVNTNNLILNSFNHIYCKYHNRNCDEDYVLST
                     FGEPLELTLKRDFSDFNYEEVVGSYRQYQVDKFDKEVCLFDTVSETIKYLHDKNIKIG
                     IATSRLKSSTLHALKIFNLEKYFQVVITAEDVVKHKPDKEPLIKVIQELNSNPENTLY
                     VGDSKFDMECAINAKVTPVLVGWHKNSDELAEKYNIKYVLNKMWDLTEIIIMSS"
     gene            complement(5567..7867)
                     /locus_tag="PLD67_00730"
     CDS             complement(5567..7867)
                     /locus_tag="PLD67_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOT21083.1"
                     /translation="MIDINKILCTEFNLKPYQVENTVKLIDEGNTIPFIARYRKEQTG
                     SLDDVVIRDLFERLTYLRNLETRKEEVIRLIEEQGKLTDELKNEILNADVLQRVEDLY
                     RPYKQKKSTRASKAKEKGLEPLAEIILAQNVTEGDLDEIAGPFVNEEKGVNSVKEAYE
                     GACDIIAETVSDNADYRKHIREVYINDAILISEAADEEEKTVYEMYYKFEEPVNKIAN
                     HRVLAINRGEKEKKLKVKLNAPDEKIINYLISKEITNENAITKDFYLSAILDSYKRLI
                     APSIEREIRTMLTERAEEEAIKVFGKNTKNLLMVPPVKDIRILAIDPSFRTGCKLAVL
                     DETGKFLEQGVMYPNEPRNEVEESQKLMADLINKFDIDVIAIGNGTASRETEMVVSDM
                     LSKYTFSKKVSYTIVSEAGASVYSASKLAQEEYPDLDVTIRGAISIGRRLQDPLAELV
                     KIDPKSIGVGQYQHDVNQTKLGQELDGVVEDCVNSVGVDLNTASPALLQYVSGITKTI
                     SKNLVKYREENGKFVNRQELMKVKMLGDKAFEQCAGFLRISDGDNPLDKTAVHPESYE
                     IAGKLLKKLGYTIQDLRSGTIRDINERIMKIPVKEDKAKTPAKNVRLKGLEALAQINF
                     KENKKSFNEKIDDRIKVLSEELNVGVPTLTDIIGELKKPGRDPREEMPKVVFRSDVKS
                     FEDMKVGMNLTGTVRNVVDFGAFVDIGVKQDGLVHLSEMSDKYIKNPMDVVQVGDTVN
                     VTIISIDKERQRIGLSMKKSLRSKND"
     gene            complement(7970..9080)
                     /gene="prfB"
                     /locus_tag="PLD67_00735"
     CDS             complement(join(7970..9004,9006..9080))
                     /gene="prfB"
                     /locus_tag="PLD67_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157593255.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOT21084.1"
                     /translation="MTDWYEITERIKKFNDILNEVGGSLDLEKLREENFTLESQMQEP
                     GFWNDQEKAQKVSQKLKHNTNRIEEFNKLKQQVEDIELFIELIKEEDDESHVPELLKN
                     MDEAEEQLESARIVALLSEEYDGNNAILSIHAGAGGTEAQDWADMLFRMYTRWIESKG
                     YSMEIYDIQTDTEAGIKSVTVFVEGQNSYGYLKSEKGVHRLVRISPFDSSNRRHTSFA
                     SVDVTPEIEDDEDIEINEDDIKIDTYRSSGAGGQHVNTTDSAIRITHIPTGIVVSCQN
                     QRSQHSNKETAMNMLKSKLLEIKRLENKEKIEDIQGKYNQIAWGSQIRSYVFHPYSMV
                     KDHRTDAETSNVQSVMDGNLDMFMNEYLKMKSRER"
     gene            complement(9214..>10478)
                     /locus_tag="PLD67_00740"
     CDS             complement(9214..>10478)
                     /locus_tag="PLD67_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004826179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOT21085.1"
                     /translation="GSVTIATNMAGRGTDIVLGGNPEFISKHKMKSKGFSEELIATAD
                     GFNETDDAEILEARRVYKQLLEESKNELRDEQKKVIEAGGLHIIGTERHESRRIDNQL
                     RGRSGRQGDPGSSRFYISLEDDLMRLFAGDKVKAIIETLKMPEDEPLEAGMLTRTIET
                     AQARVEGRNFDIRKHVLQYDNVMNKQREIIYSERRRVLLGEDLKEYISNMIDSLIDKA
                     INMYTTDEKNNENWDLNGFVKYIAETFSLLISFDGVKPEDIDKEDLRKRVRIAVDKHY
                     ARQEAEFGEETFREVERIVLLRNVDTKWMDHIDAMDQLKQGIGLRAIGNEDPVRAYQV
                     EGFDMFEEMTEAIQEDTVKMLMRVRPQEKLQRKQVARITSVSGADADSIGGRPQPLVK
                     KEKKVGRNDPCPCGSGKKYKKCCGANEK"
BASE COUNT         2714 a         2273 c         1577 g         3914 t
ORIGIN      
        1 tgtttatact aaattttagt tactacattt agtattttat cacaaaatca attaatagta
       61 tagacttttt tgatatatta cccccgctaa tcaatcatta aaatattgcg ggatatttta
      121 ttttattatc tatacgaata atctttattt ctattgaata tttttatgaa aaaatttctg
      181 atattttttc tgaaatattt tttgaaaatt ttatcatata atgtcagaag aagtctataa
      241 atacaatctg tacggcagaa atttaaactg caattgattt tttataataa tatttttgtt
      301 gccaattcta ttaaattgta caaatatttg ccacgtattt aattttattg caccgttaat
      361 aataagcata gaaagtcagt tttgattttc taaaatttaa tcaatgtaaa attttgcaac
      421 ctgttacata aaacaggtta atggggccca tgagtaattt accaatttaa cataatatta
      481 catgattaaa ttgaacctaa ttgataataa taagaataca tcttaagaga aaggagaatg
      541 aaaatggcaa caaacaacag cggaagcaac aacatagtag ttcctgaagc aaaacaagct
      601 cttaaccaga tgaaaactga aatagctaat gaattaggct taacaaatta tgagcagata
      661 gataagggaa atcttactgc aagacaaaat ggttatgtag gcggttacat gactaagaga
      721 ttggttgaaa tggctcagag acaaatgagc ggcggcggac agtttaaata gtaaataagg
      781 aaagggggac acacctctac tttgaggata tactccgggg tagaggattg tccccgtact
      841 tataaaattg gtggattaga atatccacca atttttctga tttaggccat ctgcttactt
      901 ttttttaatt cttcaataag taagggaact attttaaata aatctcctac tataccgaca
      961 tctgcaattt caaatatagg tgctgtttca tccttattta ttgcaatgat gaaatcagat
     1021 tcttccatac ctgccaaatg ctgaatagct ccggaaattc cgcatgcgat ataaagattc
     1081 ggtcttactg tctttccggt ttgtccaact tgtctgtcct ttggaaccca tcctgcatca
     1141 actgctgctc ttgatgcaga aattgttcct ccgagaagtc ctgccaattc ttcaagcatt
     1201 tgataatttt caggaccgtg cattcctctt ccgcctgata ccaatacttt tgcatcttca
     1261 atattcattc tttgttgaat tatttttgtt atatcaagta tttcaacatt ctttttgtct
     1321 gcaggaatat ctatcataaa ttgttcaata tttacaggat tgttttcgga gtatttagct
     1381 ttctgcataa ctcccggcct tacagttgcc atctgcggcc tgtgttttcc tattataatt
     1441 gtagccatca gatttccgcc aaatgcgggt ctagtcatgg caagatttaa ctgtcctttg
     1501 tttgtatcat cctctgtaat tgccagacct gtacagtctg ccgttaaacc ggttttcaat
     1561 ctagcggata ctctgggagc cacatctctt cctatagccg atgcaccgta taaaactatt
     1621 tcaggtttct tttccgttat tatcttgtgc agtgtaaaag tatatggctc tgtcatatat
     1681 tcatccagca gttcattgtc tgcgtatatt actttgtctg caccggcata gcataatttt
     1741 tttgcttctt caatcatatc ataacctaat aatactgccg taaccgttgt atttaaatct
     1801 tttgccaatt cttttgcctt gcctatcaat tcatatgcta ctttttgtac tgttctgtct
     1861 ctttgttcag caaaaacaaa aacaccttta tattcagaaa tattcatccg gttctcctcc
     1921 tatattataa atttttcttt cattttttca acaatcaatt cggctgattc ctgcggttca
     1981 aggctaaata ctttacctgc tgattttgca cccttggtaa aagatttgac aaccttggta
     2041 ggagaacctt tcaaaccgac gcattcttga gaaatatcaa catctttcaa attccaaatt
     2101 tttattcttt tttctctata ggcatcaaaa attccgccgg gagtcatata gcgaggtttg
     2161 tttaattctg ccaatgctgt tattaaacag ggtaattttg tttttaacag atggtatcta
     2221 tcttcatatt gcctttttac aataatttta tcaccgtcta ttttaatatc ttccgcatag
     2281 cttatattta ccaatcctaa atgttctgct atttgcggac caacctgtgc ggtatctcca
     2341 tctattgcct gacgtcccgt tatgattaaa tcataatcaa gattttttat ggcagctgca
     2401 agagttgttg aagttgccaa tgtatcagca cctccaaatg ctctgtctgt tatgagtatt
     2461 gcttcatctg cacccattgc caaagcttct cttagggcat cttctgcatt tggaataccc
     2521 attgttaaga ctgttacatg agcattgtat tcatctttca atctaagtgc tgcttcaaga
     2581 cctgccttgt cgtcagggtt tattatgctt ggtacgcctt ctctgattaa tgtgttagtt
     2641 ttcgggtcaa ttctaacttc tgtcgtatcg ggcacctgtt tgatacatac tactatattc
     2701 actgtctttg cctccctatt tcaacatatt tgcactgaca accattttca ttacttctga
     2761 agtaccttca taaatttcag ttatcttcgc atctctcatc attctttcaa caggataatc
     2821 tctcgtatat ccatatccgc cgtgtagctg aacagccttt gttgtaactt ccatcgctgt
     2881 ttccgatgca aacaatttag ccattgctgc ataaacacta taagcttgtc ccgcatcctt
     2941 agcgcatgct gctctgtaaa ccaaaagtct tgctgcatct actttagtct gcatatccgc
     3001 taacctaaac tgtgtatttt ggaattttgc tattgctctt ccaaattgct ttctttcttt
     3061 tacatattta gctgattcat ctatagcacc ttgagcaata ccaagcgcct gagcagcaat
     3121 tcctattctt cctgaatcaa gtgttttcat tgctatttta aatccctgtc cttcttttcc
     3181 cagaagattt tcctttggta ctatgcagtc ttcaaaaact aattcacagg tagcagagcc
     3241 tctgataccc atttttaatt cttttttccc tattgaaaag ccgggaaagt ttttttctac
     3301 tatgaaagca gaaatacctt ttgtgccttg tgatttgtcg gtcattgcaa aaattatata
     3361 gcaatccgca taggaaccgt ttgttataaa aattttcgtg ccgttcaaaa tataattgtc
     3421 accatcaagc actgccttcg tctgctgacc ggctgcatct gtgcctgctc cgggctcagt
     3481 aagcccaaat gcacctaccc attctcctga cgcaacctta gttaaatact ttttcttttg
     3541 ttcttctgtt ccaaattcat atagaggacc gccacacagc gaagtgtgtg cagatactat
     3601 aacgcctgta gttgcacata cttttgacaa ttcttcaacc gccatggcat atgtcaaata
     3661 atcgcagcct tgtccgccgt actcctttgg aaacggtatg cccataaaac catacttgct
     3721 catcttttca acagtttcaa cgggaaatct ttcttcttca tcaatttctt gtgccaatgg
     3781 ctcgacttct tccacagcaa aatttctaaa cagctgtctt gccatttcgt gttccttgct
     3841 taattgaaaa ttcatgattt cactccctaa attttttatt tgttaatttt ttaacattta
     3901 attacgcgct aacgcattaa ttatatataa ttgcttaaaa tatatcaagt gcttatttaa
     3961 atttagcagt acgtttttcc ataaaagcag tcattccttc tttttggtct tcatcagcaa
     4021 agcataggct aaataaattc gcttcaattg caattcctgt ctctatatct gtttcaattc
     4081 ccctgttcat tgcttctttt gcatatttca ttgcaacttt ggaatttgaa ataatttttt
     4141 ttgctaatgt aaatgcttct tccataaggc tgtctttgtt agttacttta ttaacgagtc
     4201 ctatcctgta tgcttcctct gcatctatat gtccgcccgt aagtattaat tcctttgccc
     4261 ttccaagtcc cactaatctt ggaagcctct gagttcctga aaatcccgga gtaattccaa
     4321 gaccgacttc cggctgtcca aacttagctt tttctgatgc aattctaatg tcgcaagcca
     4381 tgcagagttc acatccgccc ccgagagcat acccgtttac agctgcaatt acgacttttt
     4441 caagcaattc tattttcctg aaaattttag agccgtattc cgcaaatttc tttgcctctt
     4501 ctacattaag attgttcata tatgctatgt ctgcacctgc tacaaaagct ttcccttctc
     4561 ctgtaagtat aattgcggaa acttcttcgt ccttacctgc taagtcaaaa gcagaatcaa
     4621 gctcttctaa tacttcctca tttaatgcat tcaaagtttg cggactgtta attttgatag
     4681 tacaaattcc ttcatttttt tcaaataata aatttttgaa ttccatatca ataccatcct
     4741 ttctgtttgc taattgtatt atattaatga attgctatca caatattcaa actattgcaa
     4801 acacaatcaa cattgtcatt tctatgaata gcctgcggag tgtcattgct atgattatgc
     4861 tgcgtgtcat tctgagagct ttagctcgaa gaatctcatc ttataaaacc ggagatcctt
     4921 cgcttgcgct caggatgaca taataattat ttcagtcaga tcccacattt tattgagtac
     4981 gtatttaata ttatattttt ctgccaattc atctgaattt ttatgccatc ctaccagcac
     5041 cggagtaacc tttgcattta tggcacattc catgtcaaat ttagaatcac ctacatataa
     5101 agtattttcc ggattgctgt ttaattcttg gataacctta atcaaaggct ctttatcagg
     5161 cttgtgcttt accacatctt cagcggtaat aacaacttga aaatatttct ccaaattaaa
     5221 aatttttaag gcatgcaagg tggatgattt aagtcttgat gttgcaattc cgatttttat
     5281 atttttatcg tgcaaatact ttatagtttc gcttaccgta tcaaataagc atacttcttt
     5341 gtcaaatttg tcaacctgat actgtctgta gcttcctaca acttcttcat aattgaagtc
     5401 gctaaaatcc ctctttaagg ttaattcaag aggctctcca aatgtgctta aaacataatc
     5461 ttcatcacag tttcggttat gatatttgca gtaaatatgg ttaaatgaat ttaatatcaa
     5521 gttattggta ttaactaagg ttccgtcaaa atcgaatata actgtatcaa tcatttttgc
     5581 tccttagact ttttttcata cttagaccga ttctttgtct ttctttgtct atgctaataa
     5641 ttgttacatt gacggtgtct cctacctgaa caacatccat ggggttttta atatatttgt
     5701 cgctcatttc ggaaagatgc accaaaccgt cctgctttac ccctatgtct acaaatgctc
     5761 caaagtcaac aacgttcctt actgttcccg taagattcat tccaaccttc atatcttcaa
     5821 agctcttaac atcgcttctg aatacaacct taggcatttc ttcgcggggg tctctgccgg
     5881 gttttttaag ctctcctata atgtctgtta atgtaggaac tcccacattt aattcttcag
     5941 ataaaacttt tattctgtca tcaatttttt cgttgaaaga ttttttgttt tccttaaagt
     6001 ttatttgagc caatgcttcc agtcctttta gccttacgtt tttagcaggt gtttttgctt
     6061 tatcttcttt aacgggaatt ttcattattc tttcatttat gtctcttatg gtaccgcttc
     6121 ttaaatcttg aattgtatat cctaattttt tcagaagttt tcctgcaatt tcatatgatt
     6181 cgggatgtac tgcagtcttg tcgagaggat tgtctccgtc ggaaattctt aaaaaacctg
     6241 cacattgctc aaaagcttta tcacctagca tttttacttt cataagctcc tgtctgttta
     6301 cgaatttccc gttttcctcc ctgtatttaa ccaaattttt tgaaattgtc tttgttattc
     6361 ccgacacata ttgtaataat gccggagaag ccgtattcaa atcaacacct acactgttaa
     6421 cgcagtcttc aacaacgccg tcaagctcct gtcccaattt tgtctgattc acatcatgct
     6481 gatattgacc tacaccgata cttttggggt ctattttaac aagttcagcc aatgggtctt
     6541 gaagtcttct tcctattgaa atagctcctc ttatggtaac gtccaggtcg gggtattcct
     6601 cctgagccaa ttttgatgcc gaataaacag aagctccagc ctcacttacg attgtatagg
     6661 aaactttttt agaaaatgta tatttagaca gcatatcaga tacgaccatt tctgtttctc
     6721 ttgaagctgt tccgtttcct attgcaataa catctatatc aaatttattg attaaatcgg
     6781 ccatcagctt ttgtgactct tctacttcat ttcgcggctc attggggtac ataactccct
     6841 gctccaagaa cttgcctgtt tcatccaaaa cagctaattt gcatcctgtt ctgaagctcg
     6901 ggtcaatggc aagaatcctt atatccttaa ccggaggaac catcaataaa ttcttggtat
     6961 ttttgccgaa tacctttatt gcttcttctt ctgctctttc agtaagcatg gttcttattt
     7021 ccctttcaat ggaaggggca ataagccttt tataactgtc caaaattgcg gacaggtaga
     7081 agtctttagt tattgcattt tcatttgtta tttcttttga tatcaaataa ttaattattt
     7141 tttcatcggg tgcatttaac ttaactttta atttcttttc cttttcgcct ctgttaatgg
     7201 caagaaccct atgatttgca attttattta caggctcttc aaatttataa tacatttcat
     7261 agacggtttt ttcttcttcg tcggctgctt ccgatatcag tattgcatcg tttatgtata
     7321 cttctcttat atgttttctg taatctgcat tatcggaaac agtttcagct attatgtcgc
     7381 aggcaccttc ataagcttct ttgacactgt ttacaccttt ttcttcattt acaaaaggtc
     7441 ctgctatttc atcaaggtct ccttcggtaa cattttgcgc caatataatt tctgccaaag
     7501 gctcaagccc tttctctttg gcctttgatg ctcgtgtgga ttttttctgc ttatatggtc
     7561 tgtataagtc ctcgactctt tgcagcacat ctgcatttaa tatttcgttt ttaagttcat
     7621 cggtaagctt tccctgctcc tcgattaacc tgattacttc ttcttttctg gtttctaaat
     7681 ttcgtaagta tgtaagtctt tcaaataaat ctcttataac cacatcgtcc aaggagcctg
     7741 tctgctcctt gcggtacctt gcaatgaagg gaattgtatt cccttcatct attaatttga
     7801 ccgtattttc aacctggtag ggttttaaat taaattccgt acacaatatt ttgttaatat
     7861 ctatcattct tctctcccct gtacaatggt gattttccat attttctttg acattatttt
     7921 cgccgttccg agccgcaagt taacctttct aacgctcagg atgacaattt tatcgttctc
     7981 ttgatttcat tttcaaatat tcattcataa acatatctaa attgccgtcc attaccgact
     8041 gaacatttga tgtttcggca tccgtgcgat ggtccttcac catgctgtaa gggtgaaata
     8101 catatgagcg gatttggctt ccccatgcaa tttgattgta ttttccttga atatcttcga
     8161 ttttttcctt gttttccagc ctctttattt ccaaaagctt tgattttagc atgttcattg
     8221 cggtttcctt gttgctgtgc tgagacctct ggttttggca ggaaaccact atgcctgtag
     8281 gtatatgtgt tatgcgtatt gcggagtctg tagtattaac gtgctgtcct cctgcaccgc
     8341 tcgagcggta agtatcaatt tttatatcat cttcattgat ctcaatatct tcatcatctt
     8401 caatttcagg tgttacatcc actgatgcaa aggatgtgtg cctcctgttt gaagagtcaa
     8461 aaggtgatat tctgacaagt ctgtgtacac ctttttcgga tttcaaatat ccgtaagagt
     8521 tttgcccttc aacaaaaacg gtgacactct taattcccgc ttctgtatcc gtttgtatat
     8581 catatatttc catactatag cccttggatt ctatccacct ggtgtacatt cgaaacagca
     8641 tatccgccca atcttgtgcc tcggtgcctc ctgctcccgc atgaatggat aaaattgcat
     8701 tatttccatc gtattcttcg cttaaaagag caactattct tgcactttcc aattgttctt
     8761 cagcctcatc catattcttc agaagctccg gcacatggct ttcgtcatct tcttctttta
     8821 tcaattcgat gaaaagctca atatcttcta cctgctgttt taatttatta aattcttcta
     8881 ttcggtttgt gttatgcttt aacttctgag atactttttg cgccttttcc tggtcattcc
     8941 aaaatccggg ttcctgcatc tgtgattcca atgtaaaatt ttcttccctc aacttttcca
     9001 agtcaaaggg aaccacctac ctcgtttaat atatcattga actttttgat tctttcagta
     9061 atttcatacc aatcagtcaa tatatcatca tctccttctt aaatattcat aacttgtcat
     9121 cctgagcaaa gcgaaggatc tcatttttac atgcatgaga ttcttcgggc taaagccctc
     9181 agaatgacaa ttcataaaca cacagcaata acactatttc tcatttgctc cgcagcattt
     9241 tttatacttt ttcccgcttc cgcacggaca cgggtcattt ctgcctacct ttttctcttt
     9301 tttaacaaga ggctgaggcc tgcccccaat actgtctgca tctgctccgc tgacacttgt
     9361 aattcttgct acttgttttc gctgcaattt ttcctgaggt cttactctca tgagcatttt
     9421 taccgtatct tcctgaattg cttcagtcat ttcttcaaac atatcaaatc cttctacctg
     9481 atatgctctg acagggtctt catttcctat ggcacgaagt ccgattccct gtttcagttg
     9541 gtccatagcg tctatatggt ccatccattt tgtatcaaca tttctaagaa gtacaattct
     9601 ttccacttct ctgaaagttt cttctccaaa ctctgcttcc tgccttgcat aatgcttgtc
     9661 aacggcaatt ctgactcttt ttcttaagtc ttccttgtct atatcttcgg gttttactcc
     9721 gtcaaagctg attaacagtg agaatgtttc tgctatatac ttgacaaatc cgttcaaatc
     9781 ccaattttca ttattctttt cgtcggtcgt atacatgttg attgccttgt cgattaagga
     9841 atcaatcatg ttggaaatat attcttttaa gtcttctcct aacagtaccc ttcttctttc
     9901 ggaataaatt atttctcttt gcttattcat tacattgtca tattggagaa catgttttct
     9961 tatatcaaag tttcttcctt caactcttgc ttgggctgtt tcaatggtcc tggtcagcat
    10021 tcctgcttct agaggctcgt cttcaggcat ttttaatgtt tcgattattg ctttaacctt
    10081 atcaccggca aacagtctca ttaaatcatc ttccagggat atataaaacc tggatgaacc
    10141 cgggtctccc tgcctgcctg aacgacctct gagctgattg tcgatacgcc ttgattcgtg
    10201 gcgttcagta ccgataatat gaagtccgcc tgcttctatt accttttttt gctcgtctct
    10261 caattcgttt tttgattctt ccaataactg cttatatact cttcttgctt ccaatatttc
    10321 agcatcatct gtttcattga aaccgtcagc cgttgcaatt agctcttcag aaaatccctt
    10381 tgatttcatt ttatgcttag atataaattc aggatttcct ccaagcacaa tatcagtacc
    10441 tctgcctgcc atgttggttg caatggtaac agaaccaa
//
LOCUS       DAOSOW010000011         9741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_6969_length_9741_cov_3.878484, whole genome shotgun sequence.
ACCESSION   DAOSOW010000011 DAOSOW010000000
VERSION     DAOSOW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9741
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6969_length_9741_cov_3.878484"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..961
                     /locus_tag="PLD67_00745"
     CDS             2..961
                     /locus_tag="PLD67_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HOT21086.1"
                     /translation="MLTIEKIKEAQKRIAPFVYKTPLLRLNNLDEYLGCQVYTKMESF
                     QKTNSFKVRGAVNKLMTLSEEELNKGIITVSSGNHGKAVAYAATLLGRKARVVIPDNA
                     PKIKVEGIQSYGAEIIYSIPSERYNVARQLSEENGWSLIPPFDDYEIIAGQGTAGLEI
                     LEQLPDADYIVVPIGGGGLIGGLSAAVKEINPKVKVIGVEPALVPRYSESFKAGHPVS
                     LDPNSKSVADALQTLRPGERNYPIIEKYVDKIVTVHEENILKATKFLLTIGKLLAEIS
                     SSITIGAVLQKAVEFEPEDKVVFLISSGNIGIDQFNKFENIDL"
     gene            complement(1001..1579)
                     /locus_tag="PLD67_00750"
     CDS             complement(1001..1579)
                     /locus_tag="PLD67_00750"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 family protein"
                     /protein_id="HOT21087.1"
                     /translation="MFFEALILGIVIGYIRRGKLSRLSYVNFSFRFLIYISALFYLGI
                     IVINLGLYDYESFLYSTFLIGSIVLTGLFLIANISIKYMFIPLIGLALNLLSFLTNNF
                     KFPLSPDAAAKIYGAEAAELLNAGKLLFYTSSETATLSFLGNIIPIGNWFVVSIGDII
                     ASLGVVLIVQCIMADKFIQTRSKITFSKDIFR"
     gene            1811..3145
                     /locus_tag="PLD67_00755"
     CDS             1811..3145
                     /locus_tag="PLD67_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015712987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOT21088.1"
                     /translation="MLEEGSLWGPLVLQFILLFINAIFASAEIAVISMNDNRIAKMML
                     EGDKRAIRLSKLTDNPSQFLSIIQVAITLAALLGGAFAAENFAGRLTGLLINIGVNIP
                     YSVLNSVSVVLITLLLSYITLVMGELVPKRIAMKNAERLALSLSGLLYFISKLFAPVV
                     WLLTVSTNGILRLLGIDPNETDDEVTEEDIRMMVDVGSEKGSINENEKEMIQNVFEFD
                     NKTAEEVMTHRTEAVILWLDETEEEWDKKISESRHSRYPVCDGSADNVIGVLHVKEYY
                     TIEDKSRENIMKMAVKPAYYVPETVRTDVLFQNMKKSRNHFAVVFDEYGGMSGIITMN
                     DLLEQIVGDIDDDYLTPEEEEIKKIDDRTWRIKGSAYLDDVSKELGVSLPDEDFDTFG
                     GLVFGILGIIPEDGSTLELEEYGLKIVITEIKGRRLETATVYKNVDNDEDTN"
     gene            3172..3780
                     /locus_tag="PLD67_00760"
     CDS             3172..3780
                     /locus_tag="PLD67_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOT21089.1"
                     /translation="MNYNIKDILEIMPGFLLGISIHEFSHGLAADRLGDNTPRLQGRL
                     TLNPAKHLDPIGFLCLLVFGFGWAKPVMINPRNFKNPRRDDAIVSLAGPISNFLIAFL
                     FMGLIKLTINTSLSPTAIDVFYGTVMVNLMLMVFNLIPIPPLDGHHILGSIGGPKVWN
                     FYFKYREHLRFALLLLIIFRGIGRIIGPLIFILYRLLVSIFF"
     gene            3928..4503
                     /locus_tag="PLD67_00765"
     CDS             3928..4503
                     /locus_tag="PLD67_00765"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOT21090.1"
                     /translation="MESKSQLRKRVLNVRNNMSKEDVKKNSNAIMDKITSLDIYKQSK
                     VVFIYMDFKNEVITSNLIKRMLCEKKRVVIPYTDSINTVLIPSEITKESDLKQNSFGY
                     FEPKSILPVNIEEIDLVIVPGVVFDKNLNRIGFGKGYYDKILNRLKPSAKKVALAHDF
                     QVLEDIPAEEHDVKMDMIITEKNIYPFTGLL"
     gene            complement(4487..5206)
                     /locus_tag="PLD67_00770"
     CDS             complement(4487..5206)
                     /locus_tag="PLD67_00770"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOT21091.1"
                     /translation="MKGYVKSFLELLYPEKNTCFICNVYDSSIDDKYICSDCEKKLIK
                     IVPPLCIKCSKPIDYASDCLCPDCNVYEKSFEAAKSPFSYEGIIKECIYNFKYYNKPY
                     FYKFFGNLLLRYMNEINYTNFDFITSVPLHKSKMRTRGYNQSQLLAKYMSDKLSIPYA
                     DVLKRTVKTTKQSQQSKDERKKNLKGAFAIKYPHTNTKIKNRSVLLVDDVYTTGSTAD
                     ECSNTLLNFGVSKVYVITIAR"
     gene            complement(5280..7508)
                     /locus_tag="PLD67_00775"
     CDS             complement(5280..7508)
                     /locus_tag="PLD67_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HOT21092.1"
                     /translation="MERLKGTIVETIFRNNENGYTVALLETKNELITITGVFGTDISG
                     ENIEAFGKRVHHPKYGEQFQVDIYNSILPTGLEQIENYLSSGLIKGIGRHTAKKIVEA
                     FKEATFDIIQNTPEKLTEIEGIGEKKADMIAKSFQEQADVKEIMMFLQSHNISTNYNI
                     KIYKEYGKNTINVIKENPYRLAEDIYGIGFKLADKIAASLGVEKDSPYRISSGVKYIL
                     AEYAARGHTFVPYNLLVKSSANLLETSIETIEDEIRNMAFTDKIHIETIDSETVVYYL
                     PYHTAEVNVCKNLINLAGFKTDISEEDVLMYLESDEDNSINLTEMQKEAVVQSILNGV
                     TVITGGPGTGKTTIILSIIKIFEAMNKKVLLCAPTGRAAKRITESTGREAKTIHRLLE
                     YAYGENDSNLVFNKNENSPLDCHAIIVDEMSMVDILLMNNLLKALKRGTKLIMVGDVD
                     QLPSVGAGNILSDIIKSNTIRTIRLDMIFRQSAGSMIVQNAHAVNKGMMPLLNIKNSD
                     FYFIRENNINNIAKLIVELYKERLPSKYGLNPMKDIQVLSPMKKGDAGVLELNKKIQE
                     AVNPPSKLKKEKVLGKYTFREGDKVMQIKNNYQTEWKLTGNNLVTGEGIYNGDIGNIL
                     FIDEAEQEMYVQFDDEKEVVYSFNQLDELILAYASTVHKSQGSEFPVIIMPIVWAPPM
                     LLTRNLLYTAITRAKRLVVLVGQEKYMEEMIENNKIDKRFSALDYRLKSAVETYKLLT
                     DL"
     gene            7762..9354
                     /locus_tag="PLD67_00780"
     CDS             7762..9354
                     /locus_tag="PLD67_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOT21093.1"
                     /translation="MSKESFIKGAAILSVAGLFVKILGAVYRIPLTNLIGTEGIGHYQ
                     PAYNIYNLLLVVSLSGFPTAIAKMVSERRALNNYKGAYQVYKIAGWGLFLIGLVSSIF
                     VLIFARNLVTFLGFPGSYYSMLALVPALFAVPLLSAYRGFFQGMQNMTPIALSQLIEQ
                     IFRVAVGLYLAYALAGTGLEEAAAGATFGASAGGIAALILIYIMFIFSKKSIKEEISA
                     SKNNEAEPVQYVIKSLLYIAVPITIGASIAPLMGNMDSFIVSNRLAALGYTVEEYTDM
                     FGELSGTAQTLINFPQVFSTAVAMSLVPSITEAFTKKQVIRLNETANAGVKMSLIIGL
                     PCGIGLFMLAEPIIALLYQSIEPAKHASSGALLEILSIGVIFLTLVQAFTAILQSVNK
                     QFHPVKNLALGLIVKVIVSYMLIGIPNINIKGAAISTTFAYLFVALFNWYDINKTKIK
                     IDLIKVSSRALLSTAAMAVTVWISFSVILSAIGSRNLATLGSIAIAGTVYVICLFITG
                     AITKDDLELIPKGEKLKKFVRK"
     gene            9356..>9741
                     /locus_tag="PLD67_00785"
     CDS             9356..>9741
                     /locus_tag="PLD67_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOT21094.1"
                     /translation="MSIIQIIGLGAGGEDDLTVRAQKALSEKIPTFARTDRHPIVNEL
                     RKNIDIVSFDDYFEKYETFNEVYENIVNTLIEKSKQYGKINYCTAGSPYYGDIVTKKL
                     INEYKKQINIIIIDGMSFLDKCLKLSG"
BASE COUNT         3179 a         1587 c         1790 g         3185 t
ORIGIN      
        1 catgcttaca attgaaaaaa tcaaagaagc acaaaaacgc attgcaccat ttgtgtataa
       61 gactcctctc cttcgtttaa ataacttgga tgaatactta ggctgtcaag tatacactaa
      121 aatggaatca tttcaaaaga ccaattcttt taaagttcgc ggtgcggtta ataaattaat
      181 gactttatcg gaggaggaac tcaataaagg aattattact gtttcgtcgg gaaatcacgg
      241 taaagctgta gcatatgcag ctactttgtt aggaagaaaa gccagagtag taattccgga
      301 taatgctccc aagataaaag ttgaaggaat acaatcatat ggagctgaaa taatctacag
      361 tataccatcg gagcgatata atgtggcaag acaactaagt gaagaaaatg gctggagctt
      421 aatacctccg tttgatgatt atgaaattat tgccggacaa ggtactgccg gattagaaat
      481 acttgaacag cttccggatg cagattatat tgttgttccc atcggcggcg gcggtttaat
      541 cggcgggctt tctgcagcag tcaaagaaat taatccaaaa gttaaagtta tcggagttga
      601 accggcattg gttcctcgtt acagtgaaag ctttaaagca ggtcatcccg tttctttgga
      661 cccaaactca aaatcagtgg ctgatgcctt acaaacctta agaccaggag aacgtaatta
      721 ccccataata gaaaaatatg ttgataaaat tgttacggtt catgaagaaa atattcttaa
      781 ggcaactaaa ttcttattaa cgatagggaa attattggct gagatttctt cctctattac
      841 aataggtgca gtcctgcaaa aggctgttga atttgaaccg gaagataaag ttgtcttttt
      901 aatttcaagc ggcaatatcg gaattgacca gttcaataaa tttgagaata tagatttgta
      961 aaaaaataaa aaatgcgcct aagggcgctt ttttaattat ttatctgaat atatcttttg
     1021 aaaaagtaat tttgcttctg gtttgtataa atttatctgc cataatacat tgaacaatca
     1081 atacaacacc caaggaggct ataatatcgc ctatacttac cacgaaccag tttcctatag
     1141 gaattatatt gcctaaaaag ctgagtgtgg ctgtctctga agatgtatag aacaataatt
     1201 tgccggcatt taaaagctcg gctgcttctg caccgtatat ttttgctgca gcatccggag
     1261 acagcggaaa tttaaaattg tttgttaaaa acgataacaa attcaatgca agtcctatta
     1321 atggaataaa catatatttt atgcttatat ttgctattaa aaataatcct gttaaaacta
     1381 ttgagccgat aagaaatgtt gaataaagaa atgattcata atcataaagg cctaaattta
     1441 taactataat gcccaagtaa aacaaagctg aaatgtaaat caagaatcta aaactgaaat
     1501 taacatatga taatctgctt agctttcccc ttcttatata gccgataact attcctaata
     1561 ttaaagcttc aaaaaacata tttgcctcca ttaaatagag ttatactaat tctacaatat
     1621 agattaaatt atagcaactt atttttgaca aaaaatgcta gtttttgtat aaaaaattaa
     1681 taatatttat ttagttatcc tcagtataca ttgattttat aaagtttaca ttaataaatg
     1741 aatattgacg gagcaaaata tctatgttat tattcttgta attatttaac ttaattagga
     1801 ggaattaatt ttgttagaag aaggctcgtt atggggtcca ttggttttac aattcatttt
     1861 attattcatc aatgcaatct ttgcatctgc tgagattgcg gtaatatcaa tgaatgacaa
     1921 cagaatagca aaaatgatgc tggaaggcga taagcgtgct attaggcttt caaaacttac
     1981 cgataacccg tctcaatttc tttctattat ccaagttgcc atcacattag cagcactatt
     2041 agggggtgct tttgctgcag aaaactttgc aggcaggctc accggactat taataaatat
     2101 aggtgtaaat ataccttatt cagtcttaaa ttccgtttct gtagtactga taacattatt
     2161 actgtcttat ataacacttg taatgggtga acttgttcca aaaagaattg caatgaaaaa
     2221 tgctgaaaga ttagcgctgt ctttgtcggg acttttatat tttatatcaa aactgtttgc
     2281 tcccgttgtt tggcttttga cagtatccac caatggtata ttgagattat tggggattga
     2341 ccccaatgaa actgatgatg aagtaacaga agaagatatt cggatgatgg ttgatgtagg
     2401 aagtgaaaaa ggctcaatta atgaaaatga aaaagaaatg attcaaaatg tttttgaatt
     2461 tgacaataaa acagcagaag aagtaatgac tcacagaaca gaagcagtaa tattgtggct
     2521 ggatgaaaca gaggaagaat gggataaaaa aatatctgaa agcaggcact caagatatcc
     2581 tgtatgtgac ggaagcgcag ataatgttat cggagttttg catgttaagg aatattacac
     2641 aattgaggat aaaagcagag aaaatattat gaaaatggct gtaaagccgg cttactatgt
     2701 acctgaaaca gtgaggacag atgttttgtt ccaaaatatg aaaaagagca gaaatcattt
     2761 tgccgttgtt tttgatgaat acggaggaat gagcggtatt attaccatga atgatttatt
     2821 ggagcaaata gtaggggaca tagacgatga ttacttaact cccgaagagg aggaaattaa
     2881 aaaaatagat gatagaacat ggagaattaa aggctcggca tatttggacg atgtgtctaa
     2941 ggaattagga gtaagtttgc ccgatgaaga ttttgataca ttcggaggat tggttttcgg
     3001 tatattggga attattccgg aagacggaag cacattggaa ttagaggaat acggccttaa
     3061 aatcgtcata acggaaatta agggcagaag acttgaaaca gcaacagtat acaagaatgt
     3121 agacaatgat gaagatacga actaatcagt gtatactgat aaggagggac aatgaattat
     3181 aacataaaag atatattaga aataatgccg ggtttcttgc tgggaatttc aatacacgaa
     3241 ttttcgcatg gcttagcagc agacagattg ggcgataaca caccgcgatt acagggacga
     3301 ctaaccttga atccggcaaa gcatttagac ccaataggtt ttttatgttt acttgtgttt
     3361 ggattcggat gggctaagcc tgtaatgatt aatccgagaa atttcaaaaa tccaagacga
     3421 gatgatgcaa tcgtgtcatt ggcaggccct atatcgaatt ttttaattgc atttttgttt
     3481 atggggttaa taaaacttac cattaacaca tcacttagcc caaccgcaat tgatgttttc
     3541 tatggaaccg taatggtaaa tcttatgtta atggtattta acctaatccc catacctccc
     3601 ttagacgggc atcatatatt gggaagcata ggaggaccga aggtttggaa tttttatttc
     3661 aagtatcgtg agcacttaag atttgcattg cttctgttga ttatattcag gggaatagga
     3721 agaataatag gtcctcttat ttttatattg tatagattat tagtttctat atttttttaa
     3781 tgccctaacc ctattttaga ctaaagggag aaaaaaatag cgttagatcg atcatatgca
     3841 gcgcaatccc aatttgcacg accaaaggga gtgcacaagt tggtgatgga gcctgctaat
     3901 gactaaactt aatgggttgg tgatataatg gaaagcaaaa gtcaattaag aaaaagagta
     3961 cttaatgtaa gaaataatat gtcaaaagaa gatgttaaaa aaaacagcaa tgcgataatg
     4021 gacaaaataa caagcctaga tatttataaa caaagtaagg ttgttttcat atacatggat
     4081 tttaaaaatg aagtaataac atcaaatctt attaaacgta tgctttgtga aaaaaagcgt
     4141 gttgttattc catatacgga tagtataaat actgtcttaa taccctctga aataaccaag
     4201 gaatcagatt taaagcaaaa ttctttcggt tattttgagc cgaaaagtat tctaccggtg
     4261 aacatagaag aaattgactt ggttattgta cccggagttg tatttgataa gaatttaaac
     4321 agaatcggat ttggtaaggg ttattatgac aaaattttaa acaggttaaa accgtcagca
     4381 aaaaaggtag ccctagccca tgatttccaa gtgcttgaag atatacctgc agaggagcac
     4441 gatgtgaaaa tggatatgat aataaccgaa aaaaatattt acccttttac cgggctattg
     4501 taattacata gacttttgat acaccgaaat taagaagtgt attagagcat tcatcggcag
     4561 tggagcctgt tgtgtagaca tcatctacca acagcacact gcgattttta atttttgtgt
     4621 ttgtatgagg atattttata gcaaatgcac ccttcaagtt ctttttcctt tcatccttgc
     4681 tttgctggct ttgtttagtg gtttttacag tccttttcaa aacgtctgca taaggaattg
     4741 aaagcttgtc actcatatat ttcgcaagca gctgcgattg attgtagcca cgtgtgcgca
     4801 ttttagactt atgcaggggt accgatgtta taaaatcaaa gttggtgtaa ttaatttcat
     4861 tcatatacct taacaggagg ttgccaaaaa acttataaaa atagggctta ttgtaatatt
     4921 tgaagttgta gatacactct ttaataatac cttcatatga gaagggagac ttagcggctt
     4981 caaagctttt ttcatataca ttgcagtcgg ggcaaagaca atcagatgca tagtcaatgg
     5041 gcttggagca cttaatgcat agaggaggta caatttttat cagcttcttt tcgcagtcac
     5101 tgcaaatata tttgtcatcg atggagctgt cataaacatt acatataaag caagtatttt
     5161 tttcaggata taaaagttct aaaaacgatt taacatatcc tttcatttta ccccccaatt
     5221 ggtatgggta cacacctctt gtaccggtat agtcttattg gttagaggaa tatccccaat
     5281 tataaatctg tcagaagctt ataagtttcc actgcagatt tcagcctgta atcaagggct
     5341 gaaaaacgct tatctatttt attgttttca atcatttctt ccatgtattt ttcttgacct
     5401 acaagaacga ccaatcgttt tgctcttgtt atggcagtat ataataaatt tctcgttagc
     5461 agcattggcg gtgcccatac tatgggcatt ataataacgg ggaattcgct tccttgactt
     5521 ttatgtaccg ttgatgcata ggcaagtatt aattcatcta attggttaaa agaatacacc
     5581 acttcttttt catcatcaaa ttgaacatac atttcttgct ctgcttcatc aataaataat
     5641 atatttccta tatcaccgtt atatatccct tctccggtga caaggttatt acctgtcaat
     5701 ttccattctg tttggtagtt gttttttatc tgcattacct tatcgccttc gcggaatgta
     5761 tatttgccta acaccttttc cttttttaac ttggaaggag gattaaccgc ttcctgaatt
     5821 tttttattta attctaaaac acctgcatcg ccttttttca tgggagaaag cacctgtata
     5881 tctttcatgg gattcaaacc gtactttgaa ggcagacgct ctttatacag ttcaactata
     5941 agcttggcaa tattatttat attgttttct cttataaagt aaaaatctga gttttttatg
     6001 tttaacaaag gcatcattcc cttgtttacg gcatgtgcgt tctgaacgat catacttcct
     6061 gcagactgtc tgaaaatcat atcaagtctg attgttctga tggtattgct tttaataatg
     6121 tcggataaaa tattgcctgc tccaacagac ggcaattggt ctacgtctcc aaccattata
     6181 agctttgttc ccctttttaa tgcctttagt aaattgttca tcagcagtat atcaaccatg
     6241 gacatttcat caactataat agcgtggcaa tccaggggtg aattttcatt tttattaaag
     6301 actaaattgc tgtcattttc gccataagcg tattctaaga ggcgatgaat ggtttttgcc
     6361 tctcttcccg tcgattctgt tatgcgtttt gctgctcttc cggttggagc gcataagagt
     6421 acttttttat tcattgcttc aaatatttta atgattgata atataattgt tgttttaccg
     6481 gttccgggtc ctccggttat taccgtaaca ccgtttaata tggattgcac aactgcttcc
     6541 ttctgcatct cggtgagatt aattgaatta tcttcatcag attccaaata cataagaaca
     6601 tcttcttcag aaatatcagt tttgaagccg gctaaattta ttaaattttt acatacattt
     6661 acttcagcgg tgtgataggg cagataatat accacggttt cagagtcaat tgtttctata
     6721 tgtattttat cggtaaatgc catattcctt atttcatctt ctatagtttc aattgaagtt
     6781 tcaagtaaat tggcagagct ttttaccaat aaattgtaag gtacaaaagt gtgacctctt
     6841 gctgcatatt ctgccagtat atacttaact cccgatgata ttctgtaggg cgagtccttc
     6901 tcaactccta aggatgcagc tattttatcg gctaacttaa aacctatccc ataaatatct
     6961 tcagctaacc tgtatggatt ttcttttatt acatttatag tatttttacc atattcctta
     7021 taaattttta tgttgtagtt agtgctgata ttgtggcttt gcagaaacat cataatttct
     7081 ttgacatctg cctgctcctg aaagcttttt gcaatcatat cggctttttt ctcaccgatt
     7141 ccttctattt cagttaattt ttccggagtg ttttgaataa tgtcaaaggt tgcttcttta
     7201 aatgcttcta caattttttt tgcggtatgt ctgcctatac cttttattaa acctgaggaa
     7261 agatagtttt ctatttgctc taatcctgtg ggaagtatgg aattatatat gtcaacttga
     7321 aattgctcac catattttgg atggtgaact ctttttccaa acgcttcaat attttcgccg
     7381 gatatatcgg taccaaatac tcctgttatg gttattaatt cattttttgt ttccaacaaa
     7441 gcaaccgtat atccgttttc attatttcta aaaatagttt caactattgt acccttcagt
     7501 ctttccatca aattctcctt aaggaattcc cccgaactgt aataataaaa ttataccata
     7561 aaaatagtta caaggtataa aaagttgcta tttaaagaag ctaaaaataa taaaaatatc
     7621 tgctgattcg catttttctc tttaaaagaa gaataaactg tgataaacta aatttcagcg
     7681 aaaaaggtat ggggacacac cgagatagtc tctgcgggaa gaaggaatgt cccaaatata
     7741 tcttgtaaga aagggagaaa catgtctaag gaaagtttta ttaaaggtgc tgctatactg
     7801 agcgtagcgg gattatttgt aaaaatatta ggggcagtgt acaggatacc tctgacaaat
     7861 ttaataggta ctgaaggcat aggtcactat caaccggctt ataacatata taatcttttg
     7921 cttgttgtgt cactgtccgg attccccaca gcaattgcta agatggtttc cgaaagaaga
     7981 gctctaaata attacaaagg agcatatcag gtttataaaa tagcaggttg gggtttattt
     8041 ttaataggac ttgtttcatc tatttttgtg cttatatttg cccgtaatct tgttacgttt
     8101 cttggttttc ccggctcata ctattcaatg ttggcactgg ttcctgcatt gtttgcagta
     8161 cctttgctat ccgcctacag aggattcttc caaggaatgc agaacatgac tcccattgcc
     8221 ttgtcacagc tcattgagca aatttttaga gttgcagttg gtttatacct ggcatatgca
     8281 ttggcaggta caggacttga agaagctgcc gcaggtgcta ccttcggtgc atccgcagga
     8341 ggtattgcag ctcttatatt gatttatatt atgtttatct tcagcaaaaa aagtataaaa
     8401 gaagaaattt ccgcatcaaa aaataatgaa gcagaaccgg tgcaatatgt aattaaaagt
     8461 cttttataca ttgcagttcc cataaccata ggtgcttcaa ttgcaccgct tatgggaaat
     8521 atggattcgt ttatagtttc aaatcgactt gcagctctcg gatacacggt tgaagaatat
     8581 acagatatgt ttggtgaatt aagcggaaca gctcaaaccc taataaattt cccccaggtt
     8641 ttttcaacag ccgtggcaat gagcttagtt ccttcaatta ctgaagcatt tacgaaaaaa
     8701 caagttatta ggttaaatga aactgctaat gccggcgtaa aaatgtcatt aattataggt
     8761 cttccatgcg gtataggctt atttatgctt gcggagccta taattgctct gctttatcag
     8821 tctattgagc ctgcaaagca tgcaagttcc ggagcacttt tggaaatact gtcaatagga
     8881 gtgattttct taactttggt tcaagcattt acagcaatac ttcagtctgt aaacaaacaa
     8941 tttcatcctg taaaaaactt agcattgggc ttgattgtta aagttattgt atcctacatg
     9001 cttatcggca ttccaaatat taatattaaa ggagcagcaa taagcacaac ttttgcatat
     9061 ttatttgttg ctctgtttaa ctggtatgat ataaataaaa ctaaaataaa aatagattta
     9121 attaaagttt cttcaagagc acttttatcc actgcggcaa tggcagtgac tgtatggata
     9181 tccttcagtg ttatattatc agctatagga agtagaaact tagcaacatt aggctccata
     9241 gctatagcgg gaacagtata tgtgatttgt ttgtttatta ccggagcaat tacaaaggat
     9301 gaccttgaat tgataccaaa aggagaaaaa ctaaaaaaat ttgtgaggaa ataatatgag
     9361 cataattcaa ataataggat tgggagcagg tggtgaagac gacttaactg taagagctca
     9421 aaaagcttta tcagaaaaaa tacctacttt tgcaagaact gataggcacc ctatagtgaa
     9481 tgaattaaga aaaaatatag atattgtaag ttttgatgat tatttcgaaa aatatgaaac
     9541 ctttaacgaa gtttacgaga atatagtaaa tacgcttata gagaagtcaa aacaatacgg
     9601 gaaaattaac tactgtactg caggaagccc ttattacggc gatatagtaa caaaaaagct
     9661 gattaatgaa tataaaaagc aaattaatat aataataatt gatggaatga gttttcttga
     9721 caaatgttta aaactttcgg g
//
LOCUS       DAOSOW010000012         9664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_7030_length_9664_cov_3.363513, whole genome shotgun sequence.
ACCESSION   DAOSOW010000012 DAOSOW010000000
VERSION     DAOSOW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9664
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7030_length_9664_cov_3.363513"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..1145
                     /locus_tag="PLD67_00790"
     CDS             111..1145
                     /locus_tag="PLD67_00790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT21095.1"
                     /translation="MVYIFTTAYNAEKTLHKAVNSILNQTYTDFCYYLFDNGSLDDTR
                     KIIKEYANKDNRIIPLFGESNCRRVIPKIIKDIISGDNMLKNSYFATLDADDEYSPDF
                     LEKMIDFVEDNNLEVATCGTDWIDEKTGQTIKKKVLSKSLILEGKEFADKFPLYRNFI
                     VTLWGGIYSLELLKKCDFEWYHHAMNFSDTAFCMEAFRRAKRAGVLSESLHKYYISPS
                     TVSYKYNPDWFYACKYLHKISREYLLDYGEISKQNENYLCVIFLILIKYILPKIQQAD
                     VKLPEKLKSLHNIFTDDMTKYVLEHWDEVGIYSNKLDFLHEIESWLYTQDGWGLLRKT
                     VEEIITAMNI"
     gene            1182..2489
                     /locus_tag="PLD67_00795"
     CDS             1182..2489
                     /locus_tag="PLD67_00795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT21096.1"
                     /translation="MSKIIIYTPAYNAEKTLRRAVDSILNQTHKEFIYYLLDNASTDG
                     TYEIIKEYAKNDKRIIPLHNEINWVGNGILDIIKNHENDCYMCELDADDEYTPNFLEK
                     MLFFIKNENLDIAACGSDFVDMQTNKHLGMRLLEKNLILDEEEFDRYFPKYHQFMRTI
                     WGKVYSISVLRRPEYKRYKQPGYGNDTLFAMNAFYNANRVGILSESLHKYYVSPKSVS
                     YQFDKNRTKSDRVLFDAACDFLISKCGAVSDENLNYLWIVYFKAIKDTLNVLLNAQIS
                     VSDKFEYLLDIFQSRHTQELIKWPGAKAEKNRLFSQAASRMLSSSPYLVGLEVGFLCY
                     FKDIVFSILQNDEENALSQIIELIAEGTEIPDEYVESLMLLALNLSAKLESHDYFIYF
                     KKLQISLLIELSKTNEAIKEFADWDEILSDDIDFKNLKERLGI"
     gene            2486..3601
                     /locus_tag="PLD67_00800"
     CDS             2486..3601
                     /locus_tag="PLD67_00800"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HOT21097.1"
                     /translation="MKKYLLYGHGGAYNHGAEAIVKSTVDVIKKRHPDSKVILSTHFK
                     EQDIEFNMPIDEYCERDMKYVELDKNSSEKGIYDRFIYKSTLEQITEDTVCLSVGGDN
                     YCYDNWRKWKAIHERAIEEGARSILWSCSIEPSMMDNEMLDVLKSHHLITARESITYN
                     ALKDRGLDNIRLCSDVAFLLEPKEYSLPENFFVGNTVGINISPLLIRREKTEGIVIKN
                     VIKLINLVINKTDMNIALIPHVLMPMDNDLEILGKIFNRFKDTGRICFVNKNISAAEY
                     KYVISNCRLGVFARTHASIAAYSTFVPSMVIGYSVKSKGIAKDLGLEDFVLPIEEFHN
                     DCCMVSMFEELMNNEQNIKKILADKIPLYRKRAMPNY"
     gene            3633..4925
                     /locus_tag="PLD67_00805"
     CDS             3633..4925
                     /locus_tag="PLD67_00805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT21098.1"
                     /translation="MYITQCLIAKNEEANIEHCLSHLKTVVDEQIVIDTGSTDRTVEL
                     AEIMGAKVFHFDWINDFSAARNFALDKAKGDWIIFLDCDEYFSEDSVPLIRKCIKKFG
                     RQKEIEGLTTNFINIKSNGEIISAVKNVSARIFKRKSYLRYKNKIHEVLVNVNKTDDN
                     IVRADISDLLKIYHTGYDKTIVNEKNKNERNFQLLERELKNNPKDIKINYYMSRQMNI
                     EGKYEKAIEYGLKAISYIDDFYEFDYYCTIYSGIMINMLSLLKSYDEIKVIFDDAIKK
                     YPGYPDYYWLLGIAAVRGNKYEEAVNLLEKCIKLCEKYNCNAESLALGKIDDVYIELL
                     NAYNHQNNKHKIVKTAVSILKVNKYNIQVLIILLNTFLTQEKEENIVAFLNKIYDYNL
                     FKDKIYLFKASKIVDNIILVEFFRKLFNDEELKKINNI"
     gene            5079..5906
                     /locus_tag="PLD67_00810"
     CDS             5079..5906
                     /locus_tag="PLD67_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017695304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOT21099.1"
                     /translation="MRIQHNLNAMNSLRQLGTNNTNTGKNLEKLSSGFRINRAGDDAA
                     GLAISEKMRAQINGLEMATKNAEDGISLIQTAEGGLNETHAILQRMRELAVQAANGTN
                     QAEDLQSIQDEIDALADEIDRIGDSTKFNGIDLLNGGSMASDGIDFQIGANNADYESV
                     NLTIASMKASDLGVDTIDVTATGDPLGAIDAIDAAIADVSAARAGLGAMQNRLEHTIN
                     NLGVTTENLTAAESRIRDVDMAKEMMAYTKNNILTQAAQAMLAQANMQPQGVLQLLQ"
     gene            6114..6782
                     /locus_tag="PLD67_00815"
     CDS             6114..6782
                     /locus_tag="PLD67_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT21100.1"
                     /translation="MRILIVEDEVKITQALSYLFNKQKIDVDIANDGEEGLILASKDI
                     YDVIVLDIMLPGVDGIQILKTIRKDGIKTPVIMLTAKDTVDDRVFGLESGADDYLVKP
                     FATKELIARVKALARRKSTEFVGEIYEFEDIKYDVKNYLLYVGDNEYKIPLKEAMLME
                     MFIKKPRQVFTREQIIDRIWGLEADILESNIEIYVHHLRKRLENTNVKIETVRGVGYM
                     LKEK"
     gene            6782..8047
                     /locus_tag="PLD67_00820"
     CDS             6782..8047
                     /locus_tag="PLD67_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT21101.1"
                     /translation="MFRKLHLKLTAYMGIILILFMFFVSAGIYQFTKMVFEDGNKELM
                     RAEALRIYNYKKTAYTQYFFNGTLFKRYIQINPSENRRLDACYVVYDQYMIPVLSEEG
                     EIKIADHIKPLAQKAFEEKKDSYETQKIGRFNYRIYTRYFSDYTGQSVVQVYQNTVNE
                     DILWSFLRTVLIMIGITGTVILLVLSYMLTGKALAPIKETWKRQREFVADASHELRTP
                     LTVIQTNLDVVLSDEDGTVDENEIWLDNAYSETRVMARLIDQLLILAKADNNEEELDI
                     SEFSFSEVIENVCQSMEVIAAKKNIELITNIEDNIIIKADYDKMRRLAVILIDNAVKY
                     TEKGRVTVSLKTDKNKKVLIVEDTGIGINEKDLERIFDRFYRADKSRHREGGVGLGLS
                     IAKWIADKHKYSLTVESTVNVGSKFILRM"
     gene            complement(8067..8870)
                     /locus_tag="PLD67_00825"
     CDS             complement(8067..8870)
                     /locus_tag="PLD67_00825"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOT21102.1"
                     /translation="MKEKVINIAANDDVYTADIKKRLIKVLTENGYDYYEGFKEDAQL
                     NITVGGDGAFLRSVRESNFSQIPFIGVNTGTLGFYPEITPENLENFIINYRAGNYKIN
                     PVNLIECEIVSANLSNKIYAVNDIILKRIDMKTLHLNVFIASNHLEKISGDGLIISSP
                     LGSSAYNKSAGGSLVYPSLKTLQITPLSPILSNAYRCLECSIIVPPEFEIAIYPERRE
                     DYCLAILADGEIYEYDDMEHVLFRTSKKVINRLSTGTFNYWNVIKEKFL"
     gene            8999..>9664
                     /locus_tag="PLD67_00830"
     CDS             8999..>9664
                     /locus_tag="PLD67_00830"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT21103.1"
                     /translation="MFNNILKYTIQENDETVKSILTEKLKFSGRLSRKLERNGKILLN
                     GKAVKLNKSIFAGDELSIEFDEEEDRYDAVDMPLDIIYEDDDLLVVNKEPHMVVHPTR
                     SHQNDTLANGVAYYFKKNNIKRKVRFINRLDMNTSGIVLIAKNPYIHNQMVLEMKSSM
                     VEKYYYAIVEGLLEEDEGRIDVPITRLNPEDIIRTVHTSGKECITEYKAEKRYNNMTL
                     VKLK"
BASE COUNT         3555 a         1331 c         1853 g         2925 t
ORIGIN      
        1 aaataatgaa gctttttgat ataaaatatc taagtgcttg tgcttattgt aatggaatgt
       61 gtgatgattc agtaagattt gttccggctg aacaattata gggggaatat atggtatata
      121 tttttacaac ggcatataat gcggaaaaga ctttgcataa agcagttaac agcatactta
      181 atcaaacata taccgatttt tgttattatc tttttgataa tggttctctt gatgatacaa
      241 ggaaaataat caaggaatat gctaataaag ataataggat tataccactg tttggggaat
      301 ctaattgtcg cagagtaatt cctaaaataa tcaaggatat aatttcagga gataatatgc
      361 taaagaactc gtattttgca acccttgacg ctgatgatga atactcaccg gattttctag
      421 aaaagatgat agattttgtg gaggataata atcttgaagt tgctacttgc ggaacagatt
      481 ggatagatga aaaaacagga caaacaatca aaaaaaaagt acttagtaag agtttaattc
      541 ttgaaggtaa agagtttgcc gataaatttc cgctttaccg aaactttata gtcacattat
      601 ggggaggtat ttattccctt gaacttctta aaaaatgtga ttttgaatgg taccaccacg
      661 cgatgaattt ttctgatact gcattttgta tggaggcatt ccgacgagct aaacgggcgg
      721 gtgttcttag cgagagtctg cataaatact atatttcacc aagtacagta tcatataaat
      781 ataatccgga ctggttttat gcttgtaaat atttacataa aatcagtaga gagtatcttc
      841 ttgactacgg tgaaataagt aagcaaaatg agaattattt gtgtgttata tttttaattt
      901 tgataaaata tattctccct aaaatacagc aggcagatgt taaattaccg gaaaaactca
      961 aaagtttgca caatattttt acagacgata tgacgaagta tgttttagag cattgggatg
     1021 aagtcggtat atattctaat aagttagatt tcctgcatga aatagaatca tggctctata
     1081 cacaagatgg ctggggatta ttacgaaaaa ctgtagagga aattataaca gccatgaata
     1141 tctgaattaa agaaagacat aaaatgaaag gtagttgtaa gatgagtaaa attattatat
     1201 atacaccggc atataatgcg gaaaaaacgc tccgtcgtgc agttgacagt attctgaatc
     1261 aaacacataa agaatttata tactaccttc ttgacaatgc ttcaacagat ggcacatatg
     1321 agattataaa agaatatgct aaaaatgata agcgaattat tccattacat aatgaaataa
     1381 attgggtcgg aaatggaatt ttagatatta taaaaaatca tgaaaatgac tgttatatgt
     1441 gcgagcttga tgccgatgat gaatacacac caaattttct tgaaaaaatg ctttttttta
     1501 ttaaaaatga aaacttagat attgcagctt gtggcagtga ttttgttgat atgcaaacaa
     1561 ataaacatct tgggatgaga ttgcttgaaa aaaatctaat tttagacgag gaagaatttg
     1621 atagatattt tccaaaatat catcaattta tgcggacaat ttggggaaaa gtgtattcta
     1681 tatcagtatt acgtaggcct gagtataaaa gatataaaca accgggttat ggcaatgata
     1741 cactttttgc aatgaatgca ttttataacg ccaaccgtgt tggaatttta tctgaatcat
     1801 tacataaata ttatgtttct ccaaaatctg tttcttatca atttgataaa aatcgtacaa
     1861 aatctgatcg tgttcttttt gatgctgctt gtgatttttt gatttcaaaa tgtggagcag
     1921 tcagtgatga aaatcttaat tatctatgga ttgtatattt caaagcaatc aaggatacgt
     1981 taaatgtttt actaaatgct caaataagcg tatctgataa atttgaatat ttgctcgata
     2041 tatttcaaag ccgtcatact caagagctta tcaaatggcc gggagcaaaa gcggagaaaa
     2101 accggttatt tagtcaggca gcatcaagga tgctaagcag ttcaccttat cttgtcggat
     2161 tagaagtagg atttttatgt tattttaagg atattgtttt ttctattctg caaaatgatg
     2221 aagaaaatgc acttagtcaa attatagaat taattgcaga aggaacagaa atccctgatg
     2281 aatatgtaga gtctttgatg ttgctggcct taaacttgtc tgctaaatta gaatcacatg
     2341 attattttat atattttaag aaactacaaa tatccttgct tattgaactt tctaaaacta
     2401 atgaagcaat aaaagagttt gctgattggg atgagatttt atctgacgac atagatttta
     2461 aaaatcttaa agagaggttg ggtatatgaa aaagtattta ttatatgggc atggcggtgc
     2521 atataatcat ggggcggaag caattgttaa atctactgta gacgtgatta aaaagagaca
     2581 tccggacagc aaggttatcc tatcaactca ttttaaagaa caagatattg aatttaatat
     2641 gccaatcgat gaatattgtg aacgtgatat gaagtatgtt gaattggaca aaaattcctc
     2701 agaaaaagga atttatgata gatttatcta taaatccacc cttgagcaaa ttacagaaga
     2761 tacggtttgt ttatctgtag gcggtgataa ttattgctat gacaactgga ggaaatggaa
     2821 agcaattcat gaaagagcaa ttgaagaagg tgcaagaagt attttatgga gctgctctat
     2881 agagccgtca atgatggata atgaaatgct cgatgtccta aaatcgcatc atctaattac
     2941 tgcaagagaa tctattacat ataatgcact aaaagataga ggtcttgaca acatcaggct
     3001 ttgttcagat gtagccttct tattagagcc aaaggaatat tcccttccgg aaaatttttt
     3061 tgtaggtaat actgtaggaa ttaacattag tccactcctc ataagaaggg aaaagacgga
     3121 aggaatagtt ataaagaatg taataaaact gataaatttg gttattaaca aaacagatat
     3181 gaatattgca ttaatcccgc atgttttaat gcctatggat aatgaccttg aaatattagg
     3241 aaaaatattt aacagattta aggatacagg gcgtatttgt tttgtaaata aaaatataag
     3301 tgcagccgaa tataagtatg ttatttcaaa ttgccgtctt ggtgtgtttg ccaggacaca
     3361 tgcttctata gcggcatatt ctacctttgt tccgtctatg gttatcggat attcggtaaa
     3421 atcaaaagga atagctaaag atttagggct tgaggatttt gttttgccaa ttgaggaatt
     3481 tcataatgat tgctgtatgg tatcaatgtt cgaagaatta atgaacaatg aacaaaatat
     3541 taaaaaaata ttagctgata aaataccatt atatagaaaa agagcaatgc caaattatta
     3601 atatacttta ggattaagaa agggagcagg ttatgtatat aacacaatgt ttaatagcaa
     3661 aaaatgaaga agctaacata gaacattgtc taagccatct taaaacggtt gtggatgagc
     3721 aaatagtcat agacacaggt tcaacggata ggaccgtaga gctggcagaa ataatgggtg
     3781 caaaagtgtt tcattttgac tggataaatg attttagcgc cgcgagaaac tttgcattag
     3841 ataaagcgaa aggggactgg ataatatttt tagattgtga cgaatacttt tctgaagatt
     3901 ctgttccgct aataagaaaa tgtataaaaa aatttggcag acaaaaggaa atagaaggat
     3961 taacaactaa ttttattaat ataaaatcca atggtgaaat aatctctgca gtcaaaaatg
     4021 taagtgcaag aatattcaaa agaaaaagct atctcagata taagaataaa atccatgaag
     4081 tattggtaaa tgttaataaa acagacgata atattgtacg tgcagatatt agtgatttgc
     4141 taaaaattta tcacaccggg tatgataaaa cgattgtgaa tgaaaaaaac aagaatgaac
     4201 ggaattttca gttattagaa agggagttaa aaaataatcc caaagatatt aaaattaatt
     4261 actatatgag caggcaaatg aacattgaag gaaaatatga aaaagctatt gaatatggtt
     4321 taaaagctat cagctatata gatgacttct atgaatttga ttattattgt actatttata
     4381 gcggtataat gataaatatg ttatctttat taaaatctta tgatgagatt aaagttattt
     4441 ttgatgacgc tataaaaaaa taccccggat accctgatta ttactggcta ttaggtattg
     4501 cagccgtaag aggaaataaa tacgaagaag ctgtaaactt attggaaaaa tgtattaagc
     4561 tatgcgagaa atataattgt aatgcagaaa gtttagcatt agggaaaatt gatgatgtgt
     4621 atatagaatt gcttaatgca tataatcatc aaaacaataa acataagatt gtaaagactg
     4681 cggtttctat attaaaggtt aataaataca acatacaggt tttaataatt ttactaaata
     4741 cctttttaac acaagagaaa gaagaaaata tagtagcttt tttaaacaag atttatgatt
     4801 ataatttatt taaggataaa atctacttgt tcaaagctag taaaattgtt gataacataa
     4861 tattggttga attttttagg aagcttttta atgatgagga actaaaaaaa attaataaca
     4921 tctaaaaata tttttataaa acactaaata tatttattag gtggacgata tatgttatgt
     4981 aaacataaaa atatttttta tactgaggcg ccggtgtgaa aaatagaata tgtaaaatat
     5041 agggaaggat cccaagaaaa ttacacggag gtaaaaaaat gagaattcaa cacaatttaa
     5101 acgcaatgaa ctctttgcga caattaggaa caaataacac taacacaggt aaaaacttag
     5161 aaaaattatc atcaggcttt agaatcaaca gagccggtga tgatgctgcg ggcttggcta
     5221 tatctgaaaa aatgagagca caaattaatg gtctcgagat ggctaccaaa aatgctgaag
     5281 atggtatttc tttaatccag acagcagaag gcggattaaa cgaaactcac gctattcttc
     5341 aaagaatgag agaactggct gttcaggcag caaacggaac gaaccaagca gaagacttac
     5401 aaagcattca agacgaaata gatgctttag cagatgaaat tgacagaata ggcgattcaa
     5461 caaaattcaa cggcatcgat ttactaaacg gtggcagcat ggcatcagat ggtatagatt
     5521 tccaaattgg tgcaaataac gcggactatg aatctgttaa tttaactatt gccagtatga
     5581 aagcatcaga cttaggtgta gatactattg atgttacagc tacaggtgac ccattaggtg
     5641 caattgatgc tattgacgca gcaatagctg acgtatcagc agcaagagca ggtcttggtg
     5701 ctatgcaaaa cagattagag catacaatca acaacttggg agttactacc gaaaacttaa
     5761 cagctgctga atctcgtatc agagacgttg acatggctaa agaaatgatg gcttacacta
     5821 agaataacat tcttacacaa gctgctcagg ctatgcttgc acaggcaaat atgcagccgc
     5881 agggtgtact gcagttgctg cagtaaccgt taaatatatt caaaaaggat gcagactcat
     5941 gcatcctttt ttcttgacaa catttgattt attcatctat gatatacata atattaatgt
     6001 aattttaagg tttttggtat aatatagtca taactaaaaa tgaaattcga agaattgtcg
     6061 ggtaaccaaa cttggcttag gatgacgagt gtcatattat tggaggtgtt gtattgagga
     6121 tattaatcgt tgaagatgaa gttaaaatca cacaggcact gagttatttg ttcaataagc
     6181 aaaaaattga tgtggacatt gcaaatgacg gtgaagaagg actgattctt gcaagcaagg
     6241 atatttatga tgtaatagtc ttagatataa tgcttccggg agtagatggc attcaaatat
     6301 taaaaacaat cagaaaagat ggaataaaaa cacccgtaat catgcttacc gccaaggata
     6361 cggtggatga cagggtattc ggactggaat ccggagccga tgattatctt gtcaaacctt
     6421 ttgcaacaaa ggagttgatt gccagagtca aggcgctggc aagaagaaag agcacagaat
     6481 ttgttggtga aatatatgaa tttgaggata ttaagtacga tgttaaaaat tatcttctgt
     6541 atgtaggaga caatgaatat aaaatacctc ttaaagaagc aatgcttatg gaaatgttca
     6601 taaaaaaacc tcgacaggtt tttacgagag agcagattat tgatagaata tggggacttg
     6661 aagcagatat acttgaaagc aatattgaaa tttatgttca tcatttaaga aagagacttg
     6721 aaaataccaa tgtaaaaatt gaaactgtaa gaggcgtagg ttatatgctg aaggagaaat
     6781 aatgtttaga aaacttcatt tgaaattaac agcatacatg ggcattatac ttattctttt
     6841 tatgtttttt gtgtccgcag gaatatatca attcacaaaa atggtatttg aagacggcaa
     6901 taaagagctg atgagagcag aagccctgag aatttataac tataaaaaga ctgcctatac
     6961 gcaatacttc tttaacggaa ctttatttaa aaggtatata caaattaatc cttcggaaaa
     7021 caggaggctt gatgcatgtt atgttgttta tgaccaatat atgatacctg ttctttccga
     7081 ggaaggtgaa ataaaaatag cagaccatat taagccgctt gcacaaaaag cttttgaaga
     7141 aaaaaaagat tcatatgaaa cacaaaaaat aggtcgtttt aactatagga tttatacaag
     7201 atattttagt gattataccg ggcaaagcgt agtgcaggtt tatcaaaaca ccgtaaacga
     7261 ggatatatta tggtcgttct taagaacggt actgataatg ataggaatta cgggcaccgt
     7321 aattttactg gtattaagct atatgcttac aggcaaggcc ttagctccca taaaagaaac
     7381 atggaaaaga caaagagaat ttgtggcaga tgcatctcat gaactcagaa ctcctcttac
     7441 cgtaatacaa acaaacttgg atgttgtttt aagcgatgag gacggcactg ttgatgaaaa
     7501 tgaaatttgg cttgacaatg catattccga aacaagggtt atggccagac ttatcgacca
     7561 gcttttaatt cttgccaagg ctgataacaa tgaagaagaa ttagatattt ccgagttttc
     7621 tttttcggaa gttatagaaa atgtatgcca atccatggaa gtgattgctg caaaaaagaa
     7681 tattgagctc ataacaaata ttgaagacaa tataataatc aaggcagatt atgataagat
     7741 gcgtagattg gctgtaattt taattgacaa tgcagttaag tatacagaaa aaggcagagt
     7801 aacggtaagt ttaaaaacag ataaaaacaa aaaagtattg attgttgaag ataccggaat
     7861 cggcatcaac gaaaaagatt tggaaagaat atttgacagg ttctatagag cagacaagtc
     7921 aaggcacagg gaaggcggtg tagggcttgg attgagcatt gctaaatgga ttgccgacaa
     7981 gcacaagtat tcattaacgg tagaaagcac cgttaatgta ggaagcaagt ttatattaag
     8041 gatgtaataa agcaggagaa attcctttaa aggaatttct cctttataac attccagtag
     8101 ttaaatgttc ccgttgacag acggttaatt accttctttg atgtgcggaa aagcacatgt
     8161 tccatatcat cgtattcata aatttcccca tctgctagaa tagctagaca gtaatcctct
     8221 cttctttccg gataaattgc aatttcaaat tcggggggaa caattatgct gcattccaaa
     8281 catctgtatg catttgacaa aattggtgat aagggcgtta tttgcaaagt ttttaatgaa
     8341 ggatacacca aacttcctcc tgctgactta ttatatgcgg agcttcctaa gggggaggaa
     8401 attattaaac cgtcgccgct tattttttcc aggtggtttg aggcaataaa cacatttaga
     8461 tgcagtgttt tcatatctat tcttttaagt atgatatcat taacagcata aattttattg
     8521 gataaatttg cggaaacaat ttcacattca attaaattta cgggatttat tttgtaattg
     8581 cctgctctgt aattaattat aaagttttca aggttttcag gtgtaatttc cggataaaaa
     8641 cccaaggttc cggtatttac tcctataaag ggtatttggg aaaaattact ttctctcacg
     8701 cttcttagaa aagctccgtc ccctcccaca gtaatgttta attgtgcgtc ttccttgaaa
     8761 ccttcgtaat aatcatagcc gttttcggtt aaaactttaa tgagcctttt tttaatatca
     8821 gctgtataaa catcatcgtt agcagcaata tttattactt tttccttcat tattatcccc
     8881 tttactttat taatcttata tattattata agaacacaag atataatcta agaggtcaac
     8941 cctcgtcata gctgtgagag catagtcctc gtcataaaaa aactccaagg agtacataat
     9001 gtttaacaat atacttaaat atacaataca agaaaatgat gaaactgtaa agagcatatt
     9061 gacagagaaa ttaaaattct ccggtagact ctcaagaaaa ttagagcgta acggcaaaat
     9121 tctgttaaac gggaaagctg tgaaattaaa caagagtatc tttgcaggtg atgaattatc
     9181 gattgagttt gacgaggaag aagacaggta tgatgctgtg gatatgcctt tagacattat
     9241 ttatgaggat gatgatttgc ttgttgtcaa taaagagccg catatggttg ttcacccgac
     9301 aagatctcat caaaacgaca ctcttgcaaa cggtgttgca tattatttca aaaagaacaa
     9361 tattaaacga aaggttagat ttataaacag gcttgatatg aacacatcag gtattgtttt
     9421 aatagccaaa aatccatata tccacaatca gatggtgctc gagatgaagt caagcatggt
     9481 tgaaaaatat tactatgcaa tagtggaagg gttgctagaa gaagacgaag gaagaataga
     9541 cgttccaatt accagactta atcctgaaga tataatccgg acagttcata cttcaggaaa
     9601 agaatgcatt actgaataca aagcagaaaa gagatacaat aatatgacat tggttaagct
     9661 gaag
//
LOCUS       DAOSOW010000013         9603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_7082_length_9603_cov_3.695329, whole genome shotgun sequence.
ACCESSION   DAOSOW010000013 DAOSOW010000000
VERSION     DAOSOW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9603
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7082_length_9603_cov_3.695329"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="PLD67_00835"
     CDS             complement(<1..256)
                     /locus_tag="PLD67_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21104.1"
                     /translation="MKVTLEQIDELRNRVNVSYEEAKKTLEKNDGDLIKSIIELEKKK
                     GRRNEKHHGGFTAAVNKLLALRMSVKNKDGNTILNVPVVVI"
     gene            complement(393..1838)
                     /locus_tag="PLD67_00840"
     CDS             complement(393..1838)
                     /locus_tag="PLD67_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006057765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT21105.1"
                     /translation="METTKKSTNYSFALDLVEDGKDIHIKEIKKNQLPPNVTSKMLYK
                     DIVKIAWPSVVELTLTQLASMVDLMMVGGLGAWALTSVGLTIQPKFLLMTTFMALNVG
                     ATAMVARYKGAGDCKRANLILRQALMITFILSLISSLLGYIFAEEFILFMGAAEEKTL
                     AGGTVYFKIQMVGFAFMALTTTITATLRGVGNSRTALTYNLIANVVNVVLNYLLIEGN
                     FGFPRMEVAGASLATIIGQFVAFVFALIYIMRGDQYLHFRFTDGFRPDSRSLKQIITI
                     GTPAMFEQLLMRAGVIIYVRAIAGLGTVAYAVHQICMSIQALSFMNGQGFAVSATALT
                     GQSLGKNRPDMAHAYVHRTRRLGMSVSIILGIIFIFWGGNIVSLYNDAPAIVSQGARI
                     MMFVGFTQPLQSSQFILAGSLRGAGDTRATAIIIFITVLIIRPFLAIYLINVWNMGLE
                     GAWFAFIVDQTVRSALVLLRYNSGKWKSIKV"
     gene            complement(1993..2760)
                     /gene="udp"
                     /locus_tag="PLD67_00845"
     CDS             complement(1993..2760)
                     /gene="udp"
                     /locus_tag="PLD67_00845"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002597210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HOT21106.1"
                     /translation="MGTVYSDAQYHINLKEGDVGRYVLLPGDPKRCGKIAKYFDNPVL
                     VADKREYVTYTGYLDGVKVSVTSTGIGGPSAAIAMEELANIGADTFIRVGTSGGINID
                     VTGGDLVIATGAIRMEGTSREYAPIEFPAVADYGIVNALVKAAENLNKKYHVGVVHCK
                     DSFYGQHAPESKPVSYELTNKWGAWLKMGALASEMESAALFTVAAYRKVRAGSVFLVV
                     ANQERRRLGLDDPQEHDTEVAIATAIEALRILIKEEK"
     gene            complement(2874..4799)
                     /gene="thrS"
                     /locus_tag="PLD67_00850"
     CDS             complement(2874..4799)
                     /gene="thrS"
                     /locus_tag="PLD67_00850"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOT21107.1"
                     /translation="MIKIKFPDKSVREYEKGIKALDVAKSISEGLARNVVGAVYNGET
                     IGLQTALNEDGNIKFLKFEDKEGKDIFWHTSSHIMAHAIKRLWPEAKLAIGPAIENGF
                     YYDIDLEHRFVQEDFEKIEAEMKKIVKAGYELERFELPREEAIKFMEEAKEDYKVELI
                     NDLPEDAVISFYKHGDFVDLCAGPHLESTKKPKAFKLLSIAGAYWRGNENNKMLQRIY
                     GISFEKAKDLEDYLKMLEEAKRRDHRKLGKELNLFMMSEEGPGFPFFLPKGVVVKNEL
                     MKYWRKLHKKAGYVEIETPLILNQHLWEVSGHWYNYRENMYITSIDEQDFAIKPMNCP
                     GGILAYKNSMHSYKDFPMRVAEAGRVHRHELSGALHGLMRVRAFTQDDAHLYILPEQI
                     KDEIKGVIKLIDEIYKQFGFSYKLELSTRPEKFLGEIKDWDLAEASLKAALDELNLDY
                     VINEGDGAFYGPKIDFHLTDCIGRTWQCGTIQLDYQLPQRFEMEYVGADGQKHRPIMI
                     HRTAFGSIERFFGILIEQYAGAFPTWLAPVQVKILPISDKFMDYAKKVEKELDDLDIR
                     VELDSRAEKIGYKIREAQMEKIPYMLIVGEKEAESDTVSVRERQQGDIGSMSVDEISS
                     IILKKIQNRENDSPQLV"
     gene            complement(5159..6181)
                     /locus_tag="PLD67_00855"
     CDS             complement(5159..6181)
                     /locus_tag="PLD67_00855"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT21108.1"
                     /translation="MRIKAILMLLVIIISVAGCKAEDPQTTVEEIDLSQEKKSIEAFG
                     VVKPEEFMDIIIDFNSVVKEVSVNEGQYLKLNEPILLLDLSQYEADISDTVSELNIAE
                     LEYQQISMGFEDLSAEVNKLKNNIKYTEGLHQNTIKELDINKKLFEEGVISEEKYNQS
                     KLKAEEAENNLENLKFELSNAEDAIYKKTQSEKAKYAVQSEYIAQIKNRLNILQNKLN
                     KPYIAGNQIVSMYDNAVVYDIQYQPGHITDVSKKAFSIANLDSLTIEAEVVEEFIKDV
                     REGLKVKIVPAADRTIECEGRVIYVSDIAYSKNGGTVVPIKISLDNNDSFLKPNYNVD
                     VYIEVQ"
     gene            complement(6171..6854)
                     /locus_tag="PLD67_00860"
     CDS             complement(6171..6854)
                     /locus_tag="PLD67_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21109.1"
                     /translation="MEKIIEVENLNKTYMLGNVPVEILQEINFTINRGEFITIMGPSG
                     SGKSTLLYLLGGLDKPTSGKILINNKDISALSDEEQSTLRRREIGFVFQFYNLVPNLT
                     VEDNVMLPVLLDGKKIKDYREKLDEILETVGLSDRINHTPRELSGGQQQRVAIARALI
                     NEPDIILADEPTGNLDSKSSEEVMKLLQRINKVKGKTILQVSHSLETAKYSDRIINVR
                     DGKVWCNEN"
     gene            complement(6864..9386)
                     /locus_tag="PLD67_00865"
     CDS             complement(6864..9386)
                     /locus_tag="PLD67_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT21110.1"
                     /translation="MNIIVKFITKSILEKKFRSFIIIFSVTISAALFFASSALAGTMS
                     SMYEALIRMQTGKADLLIYPGRESPSSTFKILPNQVKGADYIVGTFSVGGIYKLSEEE
                     YQIERKKTELLYIKGFNIDELEKLNPINYGQKATGREFSDNHIILSEIFAKKYDYNTG
                     DSIHIEIGGVDRVLTVWGISRPTGVFQHNPQSNSIAAVMPLDTLTSLYNRRGWVDTAY
                     IVVEEGYDILNVKEEISTLYSRYVVEEPFTAEVLNEYLQFLVVPLYLMTIMVLFISVF
                     IIYSTFKVITTERLPVIGTFRSIGATRKMTDIILIGESLLYGIVGGILGVLTGMGVLY
                     LIARIMASDPYNGQLDVSIKFGLVHMLTTFILALMVATVSSWIPISRASKIPIKELVL
                     NLIDREIIKKNKKVIILTVMFAISIILSFITQNIASMIFNLLSLLIIFALVIIYIPFI
                     TSFLLKFFQQVYGAVFGNEGILAVKNLKDNKNIYNNISLLAIGISVILMINIISYSVG
                     IEVLNAYKDWKFDIMLSLNGDRNTEQVLRSIDGVEGTYGAFESFQGIKVVDTDYSIGY
                     MQGIDIIKYRDYAAFRLEGNEDVDELFKKLDDGRNIMVTLMMKDSLGLKIGDKLTLDM
                     NSGEKTYNIIGFYDSIMQNGSNAIISQKYFKTDMEQPYYNRFFVKTSKEPELVLSEIK
                     EKFMRQGVWGNSISNMEKTNYEQNSQFMIILQAFSLLAMLIGIFGVFNNYMISFLERR
                     RYIAIKRSIGLSKKQTLKMIFVEALTGGCIGATVGIIGAMFMLWIVPELMQTIGVPLA
                     IHYKSDLFAASLFTGIIISVIASVSPALKTTKLDIIESIKYE"
BASE COUNT         2851 a         2004 c         1365 g         3383 t
ORIGIN      
        1 atattactac cacgggcaca tttaaaattg tatttccgtc cttgttttta acagacattc
       61 taagagccaa aagtttgttt acggctgctg taaagcctcc atgatgtttc tcgtttcttc
      121 ttcctttttt cttttcaagc tctatgattg atttaattaa atctccgtca tttttttcga
      181 gggttttttt agcttcctcg tagcttacat taactctgtt tctgagttca tcgatttgtt
      241 ccaatgtaac tttcatagtt cctcctatat ataaatttta tttttttctt atatataagg
      301 taccaccaaa gtattaattg agctttaggt ataaattaaa attagattaa aatataaaaa
      361 ataattttaa gaaatatgaa aagtatgaat tttcagactt ttatactttt ccactttcct
      421 gaattatacc ttaaaagaac aagtgccgag cgtacagttt gatcaactat aaaagcaaac
      481 caagctcctt ccagtcccat gttccataca tttattaaat atattgccaa aaaaggtctt
      541 attatcagta ccgttataaa aataataatt gcagttgctc ttgtatctcc ggcacccctt
      601 aatgagcctg ccaaaataaa ctgggaagac tgcaagggct gtgtaaatcc tacgaacatc
      661 attattcttg caccttgtga aactattgcc ggggcatcat tgtataagga tactatatta
      721 ccgccccaga atataaagat tattcccaat ataattgaaa cagacattcc aagtcttctt
      781 gttctgtgaa cataggcatg tgccatatcg gggcggtttt tacccaggct ttgtcccgta
      841 agtgcagtag ctgacacggc aaaaccctga ccgttcataa aggataatgc ctgaatactc
      901 atacatattt gatggactgc atatgcaaca gtaccaagac ccgcaattgc tcttacgtat
      961 attatgactc cggcgcgcat caacagctgt tcaaacattg ccggagtccc tatggttatt
     1021 atttgcttga gggaacgaga atcaggccta aacccatctg taaagcgaaa gtgcaaatac
     1081 tggtcgcccc tcataatata aattaaagca aaaacaaatg caacaaattg tccgataata
     1141 gttgctaaag aagcacctgc aacttccatg cgcggaaaac caaaattccc ttcaattaaa
     1201 aggtaattta aaactacatt tacaacatta gctataagat tatatgtcaa tgctgttctt
     1261 gagttcccga cacctcttaa tgttgccgtg attgtagtgg tcaatgccat gaatgcaaag
     1321 ccaaccattt gaattttaaa atatactgta ccgcctgcca atgttttttc ttcggctgca
     1381 cccatgaaaa ggataaattc ttccgcaaaa atataaccta acaaagaaga aatcaaagaa
     1441 agtataaaag taatcatcag ggcttgccta agaattaagt ttgctctttt acaatctcct
     1501 gctcctttgt atcttgccac cattgcagtt gcgccgacat tcaaggccat gaaagttgtc
     1561 atgagaagaa atttaggctg aattgtaaga ccgacggaag taagtgccca ggctcccaaa
     1621 cctccaacca tcatcaagtc taccattgat gcaagctgag ttaaggtaag ctcaacaaca
     1681 gatggccatg ctatctttac aatgtcttta taaagcattt tggaagtcac attcggggga
     1741 agctgatttt ttttaatttc ttttatatga atatccttgc cgtcttctac caagtccaag
     1801 gcaaagctat aattagtaga tttttttgtt gtttccattg ttgctccttg tcgcataaat
     1861 aaagtcgcat agatgcgact tttttgcggt ttgagccgct aaaattcgtt ataatttatg
     1921 atatcatgat ttgtttgcaa tgtcaacaaa accggctgct aaagctacca ataaaattca
     1981 ataaatccgg tattattttt cctcttttat taaaattcgc agcgcttcaa ttgcagtggc
     2041 aattgctacc tcagtatcgt gctcctgcgg gtcatcaaga cccagacgtc ttctttcttg
     2101 gtttgccact acaagaaata cagagcctgc cctaaccttt ctgtaagctg caaccgtaaa
     2161 caatgccgct gattccatct cagatgccaa ggcccccatt ttcagccatg ctccccattt
     2221 atttgtcaac tcatagctta caggttttga ctccggtgca tgctgtccgt aaaaagaatc
     2281 cttgcaatgt acaacaccta catgatattt tttatttaaa ttttctgctg cttttaccaa
     2341 tgcattaaca attccataat cagcaactgc cggaaattct atgggggcat attctctgct
     2401 tgtaccttcc atacggattg ctcctgtggc tattaccaag tcgccgccgg taacatcaat
     2461 atttatgcct ccactggtac caactcttat aaatgtatcc gctcctatgt ttgccagttc
     2521 ttccattgca attgcagcag aaggtcctcc gattcctgtt gatgtcacac ttacctttac
     2581 tccatcaagg tagcctgtat atgtaacata ttctcttttg tcagctacta acacgggatt
     2641 atcaaagtat ttagcaattt taccgcatct tttgggatct ccgggcaata atacatacct
     2701 gcccacgtct ccttctttca aatttatatg atattgagca tccgagtata ctgttcccat
     2761 gctgcatacc tccataattt taattatcta cataatatat aaaatattgg taaaattcaa
     2821 tatctttttt attattaaaa aagaggctgc agattgcatg cctcattttt caatcaaacc
     2881 agttgtgggg agtcattttc cctattctga atctttttta aaataatgct gcttatttca
     2941 tcaacactca tgcttccaat atctccttgt tgtctttctc ttacggaaac ggtgtcgctt
     3001 tcagcttctt tttcccctac tatcaacatg tacggaattt tttccatctg agcttctctg
     3061 attttatatc ctatcttttc tgcccttgag tctaattcaa cccttatatc aagatcatca
     3121 agttcttttt caaccttttt tgcatagtcc ataaacttat ccgaaatagg cagtattttt
     3181 acctgcaccg gtgcaagcca tgtcgggaat gctcctgcat attgctcaat aagaattcca
     3241 aagaatcttt caatacttcc aaatgcggtt ctgtgaatca ttattggtct gtgtttttgc
     3301 ccgtctgctc ccacatattc catttcaaaa cgctgaggaa gttggtaatc cagttgaatt
     3361 gttccgcact gccaggttct tcctatgcag tctgttaagt ggaagtcgat ttttggtccg
     3421 tagaaagctc catcaccttc atttataaca taatctaaat tcaactcatc caatgctgct
     3481 tttagagatg cttccgctaa atcccagtcc ttgatttctc ccaaaaactt ttctggtctt
     3541 gttgaaagct ccaatttata tgagaaacca aattgcttat aaatttcatc gattaacttt
     3601 attacgcctt taatttcatc cttaatttgc tcaggaagta tatataaatg agcatcatcc
     3661 tgtgtaaatg ctcttacacg catcaagccg tgcaaagcac ccgataattc atgtctgtga
     3721 actcttcctg cttctgcaac tctcatgggg aaatctttat atgagtgcat ggaattctta
     3781 tatgctaaga ttccacccgg gcagttcata ggcttaatag caaagtcttg ctcatctatt
     3841 gaagtaatat acatgttttc tctataattg taccaatgtc cggatacttc ccataaatgc
     3901 tgatttagaa ttaaaggtgt ttctatttca acataacctg cttttttatg aagttttctc
     3961 caatatttca taagttcatt ttttactaca acgccttttg gcaggaagaa aggaaatccg
     4021 ggaccttctt cggacatcat gaataaattc aattcttttc caagttttct gtggtcacgt
     4081 ctttttgcct cttccaacat tttcaaataa tcttccaagt cttttgcctt ttcaaatgaa
     4141 ataccgtata tacgctggag catcttattg ttttcatttc ctctccagta tgctcctgca
     4201 atgctgagaa gtttaaaagc ctttggtttt tttgtgcttt caagatgagg tcctgcacac
     4261 aaatcaacaa aatcaccatg cttgtaaaat gatattaccg catcttcagg caagtcgtta
     4321 attaactcga ctttgtaatc ttcttttgct tcttccataa acttaattgc ttcttctctg
     4381 ggaagttcaa aacgttctaa ttcatagccg gcttttacaa ttttcttcat ttcagcttcg
     4441 attttttcaa agtcttcctg cacaaatcgg tgttctaaat caatatcata ataaaacccg
     4501 ttttcaattg caggtcctat ggcaagcttt gcctccggcc acagtctttt aatggcatga
     4561 gccataatat gagatgaagt atgccagaaa atatcttttc cttccttgtc ctcaaatttc
     4621 agaaatttaa tatttccatc ttcgtttaat gcggtttgaa gtccgattgt ttctccatta
     4681 tatacggctc ccaccacatt ccttgccaat ccttcactga tacttttggc aacatccaaa
     4741 gctttaattc ctttttcata ttctctgacg cttttgtcag gaaatttaat cttaatcata
     4801 actattcctc ctcttagaaa ttagtgtaat tgctattaat aacccgtcag gatgacagtc
     4861 gttaataaaa taaaatctcg ttcctaaaca tagtttaagg acgagatacc gtggttccac
     4921 cttagttgct tctcaaagga tttaaggcat ccataccaag acattacgcc atttgacttt
     4981 ctgtcttaac tccggattag ttttcaaaca gtcatacatg ggaaacttcc accaacattc
     5041 cctctctgtt aagctgtgtc tgttctactc gatccatcac agttcatttt gaccattata
     5101 ttaccggtaa atttttttgt caacagtaaa tttatatttt ttaatcttta aaatatcctc
     5161 actgtacttc aatatagaca tctacattgt aattaggctt cagaaatgaa tcattattat
     5221 ctaatgatat tttaattggc actactgtcc cgccattttt gctgtaagca atatcagaaa
     5281 catagataac acgaccctca cattctatag tcctgtccgc agccggaact atttttacct
     5341 ttaagccttc ccgtacatct ttaataaact cttcaacaac ttccgcctca attgttaagc
     5401 tgtcaagatt tgctattgaa aaagcctttt ttgaaacatc ggttatatgt cccggttgat
     5461 attgtatatc gtatactact gcattatcat acatggaaac aatttgattt cctgcaatat
     5521 acggtttatt taatttattt tgtaaaatat ttaatctgtt tttaatttga gctatatatt
     5581 ccgattgaac agcatatttt gccttttctg attgagtttt tttataaatt gcatcttctg
     5641 cattgctcaa ctcaaacttg aggttctcta aattattttc tgcttcttca gcttttaact
     5701 ttgattgatt atatttttcc tcagaaatta ctccttcttc aaagagcttt ttatttatgt
     5761 caagttcttt tattgtattt tgatgcaagc cttcggtata ttttatatta tttttaagct
     5821 tgttaacttc agcgcttaaa tcttcaaatc ccatactgat ttgttgatat tccaattcag
     5881 caatatttaa ttcgcttaca gtgtctgata tatctgcttc atattgtgac aaatccaata
     5941 agagtattgg ttcgttcaat ttcagatact gtccttcatt tacagaaact tccttaacca
     6001 cggaattaaa atcaattata atatccatga attcctccgg tttaactact ccaaaggctt
     6061 caattgattt tttttcttgg gataaatcta tttcctcaac agttgtctgc gggtcttcag
     6121 ctttgcagcc ggcaacactt attattataa caagaagcat caatattgct ttaattctca
     6181 ttgcaccaca cctttccatc ccttacatta atgattctat cactatattt agccgtttcc
     6241 agcgaatgac ttacctgtaa tatggttttt cctttcactt tatttattct ttgtaacagt
     6301 ttcataactt cttccgatga tttactgtct aaatttccag taggctcatc ggctaatata
     6361 atatccggct cattaattaa tgcacgagct attgcaacac gctgctgctg tcctccggat
     6421 aactctctag gagtatgatt tattctgtct gacaatccta ctgtttcaag tatttcatcc
     6481 aatttttccc ggtaatcctt aatcttcttc ccgtccaata ataccgggag cataacatta
     6541 tcttctaccg tcaggttagg aactaaattg taaaattgaa atacaaatcc gatttctctt
     6601 cgtcttaatg tactttgctc ctcatcactc aatgcggaaa tatccttatt atttataagt
     6661 attttccctg atgtaggttt atccaatcct cccaacaagt atagcaatgt gcttttacct
     6721 gaacctgaag gacccattat tgttataaac tctcccctgt ttattgtaaa gtttatttcc
     6781 tgcaaaattt ctacgggtac atttcctaac atatacgttt tatttaaatt ttcaacctca
     6841 ataattttct ccataactat cccctattca tatttaattg attcgataat gtctaactta
     6901 gttgttttta atgcaggact tacagaagca ataacagaga ttataattcc tgtaaataat
     6961 gaggctgcaa ataaatctga cttataatgt atggctaagg gaacacctat tgtctgcatc
     7021 aattcaggta ctatccaaag catgaacatt gcacctataa ttccaactgt tgcaccaata
     7081 catccgcctg tcaaagcctc cacaaatatc attttcaatg tttgcttttt gctcagtcct
     7141 atggaacgct taattgcaat ataacgtctg cgctctaaaa aactaatcat ataattgtta
     7201 aagaccccga agatacctat gagcatagct aaaagagaaa atgcctgaag gataatcatg
     7261 aattgactat tttgctcata atttgttttt tccatgtttg aaattgaatt accccatacg
     7321 ccctgtctca taaacttttc cttgatttct gataggacta actccggctc cttagatgtt
     7381 tttacaaaaa atctgttata atatggctgc tccatatctg ttttaaaata tttctgagat
     7441 ataattgcat tactcccgtt ttgcattatt gaatcgtaaa aaccgattat attataggtt
     7501 ttttcgccgg aattcatatc taatgtaagt ttgtcgccta tttttaatcc caggctgtct
     7561 ttcatcataa gagtaaccat tatatttctg ccgtcatcaa gctttttgaa cagctcgtca
     7621 acatcttcat tgccctccag acgaaaagct gcataatccc tgtacttgat gatatcaata
     7681 ccctgcatat atccaatgct gtaatcggta tccacaactt ttattccttg aaaactttca
     7741 aaggctccgt aagttccttc cactccgtca atacttctaa gtacctgttc cgtatttcta
     7801 tcgccgttta aggaaagcat tatatcaaac ttccagtcct tataagcatt taacacttct
     7861 atacctacgc tgtaactgat tatattaatc atgagaataa ccgaaattcc aattgccagc
     7921 aatgatatat tattatatat atttttgtta tcctttaaat ttttaaccgc aagaattcct
     7981 tcatttccaa atacagctcc atagacttgc tgaaaaaact ttaataaaaa tgatgtaata
     8041 aaaggaatat agattattac aagagcaaat attatcaata aagacaaaag attgaatatc
     8101 attgatgcta tattttgtgt tatgaaagat aaaattatcg atatagcaaa cataacagtc
     8161 aatataatga cctttttgtt ttttttgatt atttctctat caatcaaatt caataccaat
     8221 tcttttattg gtatttttga agccctactt attggaatcc aagaactgac agttgcaacc
     8281 ataagggcta aaataaaggt cgtcagcata tggactaagc caaatttaat cgatacatcc
     8341 aactgtccgt tataagggtc tgaagccatt attcgggcaa taagatataa aactcccatt
     8401 cctgttaata ctcctaatat tccccccaca attccataca ataagctttc gcctatgagt
     8461 ataatatccg tcatttttct tgtagcacca atgcttcgaa aagtacctat gacaggaagc
     8521 ctttctgttg ttattacctt aaatgtggaa taaataataa aaacactgat aaataacacc
     8581 ataatggtca ttaaatacaa aggtacaact aaaaattgca gatactcatt caaaacttcc
     8641 gctgtaaaag gctcctctac aacatatctg ctatacaagg tgcttatttc ttcttttaca
     8701 tttaaaatat catatccttc ttcaacaaca atataagcag tatcaaccca acctctccga
     8761 ttatataaag aagtaagagt atctagaggc attactgcgg ctatagaatt tgactgcgga
     8821 ttatgctgaa atacacctgt aggtcttgaa ataccccata cggttaaaac tctgtcaaca
     8881 ccgccgattt ctatgtgtat actgtctccc gtattataat catatttttt tgcaaatatt
     8941 tcgcttaaaa taatatgatt gtctgaaaac tccctgcctg ttgccttttg accataattt
     9001 atgggattaa gtttttccaa ttcatctata ttaaaccctt ttatatatag cagctctgtc
     9061 ttttttcttt caatttgata ttcttcctct gacaatttat aaattccgcc aaccgaaaaa
     9121 gttcctacaa tataatcagc accctttact tggttgggca atattttaaa agtggaagaa
     9181 ggtgattccc tgccgggata aatcaataaa tctgctttgc cggtctgcat tctaataaga
     9241 gcttcataca tggaactcat ggtacctgcc aaagcacttg aagcaaaaaa taaggctgct
     9301 gaaattgtaa ctgagaaaat aattataaaa gacctgaact ttttttctaa aatacttttt
     9361 gttataaatt taacaataat attcattaaa acaccgccaa aaaaatacat tagctttaat
     9421 attttccatt aattgttata ccatatatgc atatttatta caagaatttt tttatttttt
     9481 tgttgctctt aaaagcgctt ttattatgaa ttcattatat gagaagaaaa ataaaataaa
     9541 aagtgactta agtcatcata taagtcacat ttttattact gcataaagat gggttagtta
     9601 atc
//
LOCUS       DAOSOW010000014         9372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_7295_length_9372_cov_3.922078, whole genome shotgun sequence.
ACCESSION   DAOSOW010000014 DAOSOW010000000
VERSION     DAOSOW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7295_length_9372_cov_3.922078"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..1290)
                     /gene="serS"
                     /locus_tag="PLD67_00870"
     CDS             complement(16..1290)
                     /gene="serS"
                     /locus_tag="PLD67_00870"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004831860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOT21111.1"
                     /translation="MLDIKRIRNNPNEVEELLKRRNSELSLDKVLALDKVRREKLVEV
                     ENMKAEQNKVSKQVPLLKKEGKDVTEILQKMKELAEKVKVLDGQVKEIDEKIEEELLN
                     IPNTPNPDITVGASDADNKEIRRWGEPRKFDFELKAHWDLGVDLDILDFERATKITGT
                     RFSMFKGEGARLERAIIAYMLDKHTLDHGFTEISPPFMVNRDSMIGTGQLPKFEEDMF
                     KLNQREYYLIPTAEVPVTNIYRNEVLDEKDLPMYMTAYTPCFRAEAGSAGRDTRGLIR
                     NHQFDKVEMVMYAKPEDSYNQLEILTNFAEEILKGLGLPYRVVELCTGDIGFSSAKTY
                     DLEVWMPSYNRYVEISSCSNFEDFQARRANIKFRPAEKGKLEYIHTLNGSGLAVGRTF
                     AAILENYQNEDGTVTIPNVLRDYMRGLEKITK"
     misc_binding    complement(1323..1583)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1622..2176)
                     /locus_tag="PLD67_00875"
     CDS             complement(1622..2176)
                     /locus_tag="PLD67_00875"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOT21112.1"
                     /translation="MIGKKIRNIRKNKNMTIVELSEKINVTSGYISQIERDLISPSLA
                     VLKRLSDALDVPLSVLFSEESSNDVITIPKEERTKVKLNNINVELEFITPILKNSHKQ
                     SEFEAFLFKMKPKTWISNHSITHESKEYIFVTQGEIECHVGEKIYTISKGDSIIVPEN
                     NNHMYYNKNDDVSEALCIISPSIH"
     gene            2439..4673
                     /locus_tag="PLD67_00880"
     CDS             2439..4673
                     /locus_tag="PLD67_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT21113.1"
                     /translation="MADNRVYLEEIKYLDEIINLLKNKLEYEKNQLENKKSDLIESRR
                     EMWENTTHSSADFDKLTDLNQYLSALQAQTFTYAELAKRILKHEKMLDSPYFARIDFT
                     EEGYDDIEKIYIGLFNLMDDETHEIKIYDWRAPISGLYYRNEVGPVEYKAPAGLIKGY
                     VSLKRQFKITKGKMEYFIDSNINILDEMLMVALSKNMTSKMKTIVETIQKQQDLIIRD
                     LYNDLLVVQGVAGSGKSSVALHRIAYLMYQGLNLKLRANNVIVISPNPLFSKYISNVL
                     PELGEDSINEYTFENIFHKLFGNKLSMKSKSENFENIITAETEEQRNFLRSYDDFKGS
                     FVFSKIIDRFIEYYEHKLINFEDVYYNGKIIEKRELAKAFLLSGKLDMPTAKKLKIIE
                     SRILEKVRENRIERRNKIEKAVNKLNNHEFEIMSFTRLLSAKETLALTERLKKFTEFD
                     VFELYKKLFADIILFKKISKGLELPENIDEIISYTNTTLSDSYNIPYADGIALTYMKI
                     KAEGCDLFSDIKQVVVDEAQDYYPIHYLILKNLYKYARFTIVGDINQSIEKSNDLSLY
                     DDIISIFNFEKSSKVFLNKSYRSSYEIGKFSAKLSGDENYAEFFKRNEEEPVIFKFKD
                     INNLRDEIIKTIEKYKTEGFSSIAIICKDRKEASDLYFKLANKIKIKLIDYLDYDSVL
                     EGTLILPVYLAKGLEFDAVIVYEANDKHYNNEFDKKLLYVACTRALHRLSLFYTGNLT
                     KFLD"
     gene            complement(4725..4795)
                     /locus_tag="PLD67_00885"
     tRNA            complement(4725..4795)
                     /locus_tag="PLD67_00885"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4761..4763),aa:Gly,seq:ccc)
     gene            complement(4802..4874)
                     /locus_tag="PLD67_00890"
     tRNA            complement(4802..4874)
                     /locus_tag="PLD67_00890"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4839..4841),aa:Ala,seq:ggc)
     gene            complement(4965..5037)
                     /locus_tag="PLD67_00895"
     tRNA            complement(4965..5037)
                     /locus_tag="PLD67_00895"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5002..5004),aa:Thr,seq:cgt)
     gene            5176..5454
                     /locus_tag="PLD67_00900"
     CDS             5176..5454
                     /locus_tag="PLD67_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21114.1"
                     /translation="MDDYNQYYDTMNDYMYYDDINKDVYSSQYDMYDRRFDNRFDNRF
                     DNRFDRRFDRRYDRFFYDPMRYYRFPFCDRYGRCENPIWWLFWPFFFQ"
     gene            complement(5486..6046)
                     /gene="pyrE"
                     /locus_tag="PLD67_00905"
     CDS             complement(5486..6046)
                     /gene="pyrE"
                     /locus_tag="PLD67_00905"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOT21115.1"
                     /translation="MIDVIEILKECGALLEGHFLLSSGKHSNKYCQCAKLLQYPDKAE
                     KVISLVYEKVKSLNLDVIVGPAMGGITAAYELGRQLGIKTIFTERENNVMTLRRGFEI
                     QPGDRVLIMEDVVTTGKSSMETAKVLEEAGGIVVGIGCIVDRKVCEIQFPIYSSIELF
                     FETYDDKNCPLCEMGSKPIKPGSRKF"
     gene            complement(6066..6968)
                     /locus_tag="PLD67_00910"
     CDS             complement(6066..6968)
                     /locus_tag="PLD67_00910"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOT21116.1"
                     /translation="MANLKTEAMDLKFKNPIIAASGTFGFGEEFSQIYDLSILGGICS
                     KGLTLTPRSGNKGIRLWETPMGLMNSIGLENPGVDKFITEELPRMKTYNTIIFANLGG
                     HCEEDYLEGIYKLNDTNVDAVELNISCPNVKSGGMAFGVKSSVAYDIVSRVRKICKKP
                     LMVKLSPNAENIPDMAVACESAGADAISLINTIQAMAIDIKNRKAVFENVYAGLSGPC
                     VKPIALRMVHEAAKMVNIPICGLGGIMNAIDVIEFIMAGATLVQIGTANFIKPDIAVD
                     ILNDLEEFMDNENIKSLEEIRGII"
     gene            complement(6978..7706)
                     /locus_tag="PLD67_00915"
     CDS             complement(6978..7706)
                     /locus_tag="PLD67_00915"
                     /inference="COORDINATES: protein motif:HMM:NF000798.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOT21117.1"
                     /translation="MKVLVTAVAENGQISKNIYRLSVKFDSEIKPGQFFMLKTTDNAF
                     LLPRPISVNDVNENLVSFLYRLEGQGTKKISSLRKNDEIQIFGPLGKGFEPDKLLGRV
                     AVIGGGIGVAPLLYLCKVLGKKADVYLGYRDKENMYIIDEFKSSARRCIVVTEDGSFG
                     EKGYVTDYISYDDYNVVVTCGPEIMMSKIATICRDKKIKCYVSLERRMACGMGACLGC
                     TVETKTGNKRACKEGPVFDSEELI"
     gene            complement(7729..8154)
                     /locus_tag="PLD67_00920"
     CDS             complement(7729..8154)
                     /locus_tag="PLD67_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOT21118.1"
                     /translation="MLNIDSVEKGLVIDHIKAGKAMEIYHYLNLDKMDCSVAIIKNAK
                     SKKYGKKDIIKVENNLDMDLKVLGFIDPNITINVIDNSVIIDKINLELPQEVENVVKC
                     KNPRCITSIEQEIVHKFRLADREKGIYRCVYCDTAYDKK"
     gene            complement(8154..9077)
                     /gene="pyrB"
                     /locus_tag="PLD67_00925"
     CDS             complement(8154..9077)
                     /gene="pyrB"
                     /locus_tag="PLD67_00925"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOT21119.1"
                     /translation="MLKGRSLIEPMDLSLAELDDILNLADRIMHDPAAYADKCKGKVL
                     ATLFYEPSTRTRLSFESAMLRLGGSVMGFSSAESSSAAKGESVADTIRTVACYADIAA
                     MRHPKEGAPMVAAMKANIPVINAGDGGHQHPTQTLTDLLTIQTLKGKINNFTIGLCGD
                     LKFGRTVHSLIKALSRYENIKFILISPDELRVPDYIREEILMKNGIEFMEVERLENVI
                     GELDVLYMTRVQKERFFNEADYIRLKDSYIVDNKKMSNAKKDMILMHPLPRVNEISVE
                     VDNDPRAAYFIQAKCGMYVRMALILKLLGVE"
BASE COUNT         2899 a         1808 c         1414 g         3251 t
ORIGIN      
        1 aataacaata ccttgttatt ttgttatttt ttccaaacct ctcatgtaat ccctcaagac
       61 attaggaata gtcactgtac catcttcatt ctgataattt tcaagaatag cggcaaatgt
      121 ccttcccaca gcaagtccgg agccgtttag ggtatgaata tactctaatt tacctttttc
      181 tgcaggtcta aacttgatat tggctcttct agcttgaaaa tcttcaaaat tcgagcaaga
      241 agaaatttca acatatctgt tgtagcttgg catccatacc tccaaatcat aggttttggc
      301 tgagctgaaa ccgatatctc ccgtacatag ctctacaacc ctgtaaggaa gacctaatcc
      361 ctttaaaatt tcttcagcaa aatttgtcag tatttcaagc tggttataag aatcttcagg
      421 tttggcatac ataaccattt caaccttatc aaattgatga tttcggatta gacctcttgt
      481 atctctcccg gcagaacctg cttctgccct gaaacatggc gtataagcag tcatatacat
      541 tggaaggtcc ttttcatcca gcacttcatt tcggtatata tttgttacgg gaacttcagc
      601 agtaggaatt aaataatatt ccctttgatt aagcttaaac atgtcttctt caaatttagg
      661 aagctgtcct gttcctatca tactgtccct atttaccata aagggaggag aaatttccgt
      721 aaagccgtgg tctaaggtgt gcttgtcaag catatatgca ataattgccc tttccaatct
      781 tgctccttct cccttgaaca ttgaaaaacg agtgcccgta atttttgttg ccctttcaaa
      841 atcaagaata tccaagtcta cgcctaaatc ccagtgagcc tttaattcaa aatcaaactt
      901 tctcggctcc ccccatcttc ttatttcctt gttgtctgca tctgaagcac cgacagttat
      961 gtcgggattt ggagtgtttg gaatattcaa cagctcttct tcgattttct catcaatttc
     1021 ttttacctgg ccatccagaa cctttacttt ttccgccaat tctttcattt tttgaagaat
     1081 ttccgtcaca tcttttcctt cttttttgag aagaggaacc tgtttagata ctttattctg
     1141 ctctgccttc atgttctcca cttcaacaag tttttctctc ctcactttgt ctaatgcaag
     1201 aaccttatcc aaactaagtt cggaatttct tcttttcaac agttcttcca cctcattagg
     1261 gttgtttctt attcttttga tatctaacat tttttacctc cataaataaa aactcccgcc
     1321 tctttagaga cgagagatac tcgcggtgcc accctaatta ttatactaat ggggggacat
     1381 tccttcatcc ccggagactt tccctaaaaa aggtgtgccc ccataccata atataatcac
     1441 tttaagaagt taacgccttc aacgtctaag ttttttaccc ttagatactc cggaacggat
     1501 tcaacaacta ttataccggc tctcaccacc cgtcggctct cttaaataag tcgctgttta
     1561 ctgctttcct tcattgttta tatggataat ataataacat tataactttg tcaagcttta
     1621 tttaatggat tgaaggcgat attatgcaca aggcttctga cacatcatca tttttattgt
     1681 aatacatatg attgttgttt tcaggaacaa ttatgctgtc tccctttgat attgtgtata
     1741 ttttttctcc tacatgacac tctatttcgc cctgtgtaac aaaaatatac tctttagatt
     1801 catgtgttat actatgattg cttatccatg tttttggttt cattttaaac agaaaggctt
     1861 caaactccga ttgtttatga ctatttttca atattggcgt aataaactca agttccacat
     1921 taatattgtt caatttaact ttagttcttt cttcttttgg tattgttata acatcattgg
     1981 aagattcctc cgaaaataat actgaaagag gtacatccaa tgcatcagac agtcttttta
     2041 ataccgctag agaaggagat attaaatctc tttctatttg tgatatatat cctgatgtta
     2101 cgttaatttt ctctgacaat tcaacaatcg tcatattttt attctttcta atattcctga
     2161 tttttttccc tatcattata ataccccctg tactgcttat actaatatta tacactaaat
     2221 aaaacttatg tcacgtaaat tttcacattt tataccatta aatggtatat taaaatgcat
     2281 tgcagtttct gctcttgttt taatgatagt cagcaaagac aaatatttta cttttattgt
     2341 agaaagttgg atttttgctt ttcatataat actaattatg atataattat ctattaatac
     2401 tttgtaatta ttcaattata tgcataaggg gatatgaaat ggctgataac agagtgtatt
     2461 tggaagaaat aaaatatttg gatgaaatta taaatttgtt aaaaaataag ttggaatatg
     2521 aaaaaaacca acttgaaaat aaaaaatccg atttaattga gtcaagaaga gaaatgtggg
     2581 aaaatacaac ccattcatct gccgattttg ataaactaac tgaccttaat cagtatttga
     2641 gtgcccttca ggctcaaact ttcacctatg ccgagcttgc aaaacgtatt ttaaagcatg
     2701 aaaagatgct tgacagccct tattttgcaa gaattgattt caccgaagaa ggatatgatg
     2761 atattgaaaa aatatatata ggtcttttta atttaatgga cgatgaaacc catgaaatta
     2821 aaatctatga ttggcgtgca cccatatccg gcctatatta ccgaaatgaa gtaggtcctg
     2881 tagaatacaa agctcctgcc ggattaataa agggctatgt ttcattaaaa cggcaattca
     2941 aaattacaaa aggtaaaatg gaatacttca ttgacagcaa cattaacatt cttgatgaaa
     3001 tgctgatggt tgctctatca aaaaatatga cctccaaaat gaagacaata gtagaaacaa
     3061 tacaaaaaca acaggactta ataataagag acctgtataa cgacttattg gttgtacaag
     3121 gggttgcagg cagcggcaag tcatcggtgg cgctgcacag aattgcatat ttgatgtatc
     3181 aaggtttaaa cttaaaacta agagccaata atgttatagt tatttcacct aatccgttat
     3241 tctcaaaata tatatcaaat gttcttcctg aattaggaga agatagtatc aatgaatata
     3301 cttttgaaaa tatatttcat aaactatttg gaaataaact atccatgaaa tcaaaaagtg
     3361 aaaactttga aaatattatt actgcagaaa ctgaagaaca aaggaatttt ctgaggtcat
     3421 acgatgattt taaaggctca tttgtttttt caaaaattat agatagattt atagaatatt
     3481 atgagcataa gcttataaat tttgaagatg tttattacaa cggaaaaata atagaaaaac
     3541 gcgagcttgc aaaagctttt ctgttaagcg gaaaacttga tatgcctaca gccaaaaaac
     3601 ttaaaataat tgaaagccga atattggaaa aggttcgcga aaataggatt gaaaggcgta
     3661 acaaaattga aaaagcagta aataaattaa ataatcatga atttgaaata atgtctttta
     3721 caagattgct atcagctaaa gaaaccttag ctttaacgga aagattgaag aaatttacgg
     3781 aatttgatgt tttcgagctt tataaaaagc tgtttgcaga tattatatta tttaaaaaaa
     3841 tttcgaaagg tctagagctt ccggagaata ttgatgaaat aataagttat acaaatacaa
     3901 ctttaagcga ttcttacaat attccttatg ctgatggaat agctctcact tatatgaaaa
     3961 ttaaagcaga gggttgtgat ctgtttagcg atataaaaca agttgttgtt gatgaggccc
     4021 aggattatta ccccattcat tatttaattt taaaaaattt atataaatat gcaagattta
     4081 caatagtagg cgatataaat caatcaatag aaaaaagcaa cgatttgtcc ttatatgatg
     4141 atattatttc tattttcaat tttgaaaaga gcagtaaggt ttttctgaat aaaagttaca
     4201 gaagttcata tgaaataggc aagttcagtg caaaattatc gggagatgaa aactatgcgg
     4261 aattttttaa aagaaatgaa gaagagcctg ttattttcaa atttaaagac attaacaacc
     4321 tccgggatga aattataaaa actattgaga aatataaaac tgaaggattt agctcaattg
     4381 caataatatg taaagataga aaggaagcct cagacttgta ttttaaactg gctaataaaa
     4441 taaaaataaa attgatagac tatttagatt atgacagtgt tttggaaggt accttaatac
     4501 ttcctgttta tttggcaaaa ggtctggaat ttgatgctgt tattgtatat gaagcaaatg
     4561 ataaacatta taataacgaa ttcgataaaa aacttttata tgtcgcatgt acaagagcac
     4621 ttcacagatt atcactgttt tatacgggaa atttaacaaa atttttggac taaaaaatcc
     4681 gcttaaccct taagcggatt tgcttatata tggtcatgtc ataaagcggg tgatgggaat
     4741 cgaacccaca cagccggctt gggaagccgg aactctgcca ctaagttaca cccgcaataa
     4801 atggagataa ggggtctcga acccctggcc tcttgaatgc cattcaagca ctctcccaac
     4861 tgagctatat ccccaaaagt gtatgtatct taattttaag aaaaaatact atatataatt
     4921 taatatattt tttttcattt gtaaagttaa tcttttaata aaaaagctac tgaggggatt
     4981 cgaacccctg acctactgat tacgaatcag ttgcgctgcc tgctgcgcca cagtagcatg
     5041 tatataattt aatacttttt tggcaatttg taaacagtaa attttgtaag actaaatttc
     5101 cgggaaataa tcgtaacatg agaggttgta acacatatat tataatatat aaattttata
     5161 tagaggagag tatatatgga cgattacaat cagtattatg ataccatgaa cgattatatg
     5221 tattatgatg atattaataa agatgtatac agcagtcaat acgatatgta tgacaggaga
     5281 ttcgacaaca ggttcgacaa caggttcgac aacaggttcg acagaagatt cgatagaaga
     5341 tatgacagat ttttttatga cccgatgagg tattatcgat tcccattctg tgacaggtac
     5401 ggaagatgtg aaaatcctat ttggtggctg ttctggccct tcttttttca ataatcagta
     5461 tgacaaaggg acgctctgcg tccctttaaa atttgcgact tcccggtttt attggctttg
     5521 agcccatttc acataggggg caatttttat catcataggt ttcaaagaat aattctatcg
     5581 agctgtaaat aggaaattga atttcacata cttttctatc tacaatacat ccgattccta
     5641 ctactatacc gcctgcctct tcaagaacct tggcagtttc catggatgat ttcccggttg
     5701 ttacaacatc ctccattatt aaaactctgt caccgggttg tatttcaaaa ccccttctaa
     5761 gtgtcattac attattttct ctctcagtaa atattgtttt tatacctaat tgtctgccta
     5821 gctcataagc ggctgttatt cctcccatag caggtccgac aataacatcc aaatttaatg
     5881 attttacttt ttcatataca agagaaataa ctttttctgc cttgtccgga tattgcagaa
     5941 gtttagcaca ttgacaatat ttgttgctgt gcttgccgga tgaaagcaag aaatgacctt
     6001 caagcaatgc accgcattct ttcaatattt caattacatc tatcatatta ccgcctcctg
     6061 tggctctaaa taatccctct tatttcttct agggatttaa tattttcatt gtccataaat
     6121 tcttccaaat cattgagaat gtctactgca atatcaggtt taataaagtt cgctgttcct
     6181 atttgcacca atgttgctcc tgccataatg aattcaatta catcgattgc attcattata
     6241 cctccaagac cgcaaatggg aatattaacc atttttgcag cttcatgtac cattcgaaga
     6301 gcaataggtt ttacacatgg tcctgaaagt cctgcataaa cattctcaaa tacggctttt
     6361 ctgtttttta tatcaattgc catagcttga attgtattaa ttaatgaaat tgcatctgct
     6421 cctgccgact cacatgctac agccatatcc ggaatatttt ccgcatttgg tgatagttta
     6481 accataagag gctttttaca tatcttacga actcttgata ctatatcata agcaacggaa
     6541 gatttcactc caaaagccat gcccccgctt ttaacattcg gacaggaaat gttaagctct
     6601 acagcatcca catttgtatc attcagctta tatatgcctt ctaaataatc ttcctcgcaa
     6661 tgtcctccaa gattggcaaa aataatagta ttatatgttt tcatcctggg aagttcttct
     6721 gttataaatt tgtcaacacc cgggttttca agtcctatgc tgttcataag ccccattgga
     6781 gtttcccaga gtctgattcc tttattcccg ctccttggag ttaatgtaag gcctttgctg
     6841 caaattccgc caagaattga taaatcgtaa atttgagaaa attcttctcc gaaaccaaaa
     6901 gttcctgatg ccgctattat ggggttttta aatttaagat ccattgcttc ggtttttaaa
     6961 tttgccatat ttctcctcta tattaattct tcactgtcaa atacaggacc ttccttgcaa
     7021 gctcttttat tgcccgtctt cgtttcgacc gtacacccca aacaagctcc cataccgcat
     7081 gccatccgcc tttccaacga tacatagcat ttaatttttt tgtccctgca tatagttgca
     7141 atcttactca tcattatttc cggcccgcag gttacaacaa cattataatc atcataagaa
     7201 atataatccg ttacgtaacc tttttctcca aagcttccat cctccgtaac aacgatgcat
     7261 ctgcgggctg aagatttaaa ttcatcaatt atatacatat tttctttatc cctgtatcct
     7321 aaataaacat ctgctttttt acctaagact ttacataaat acaatagtgg agcaacacct
     7381 atgcctcctc ctattactgc aactctgcct aacagtttat ccggctcaaa gccctttccc
     7441 aagggaccga atatctgaat ttcgtcattt tttcgcaggc tgcttatttt ttttgttcct
     7501 tgcccttcca gtctatataa aaatgaaaca agattctcat ttacatcatt aacgcttatg
     7561 ggtctcggaa gcaaaaatgc attatcagtg gttttcagca tgaaaaattg tccgggtttt
     7621 atttccgaat caaattttac cgaaagccta taaatatttt tggaaatttg cccgttttcc
     7681 gcaacagcgg ttactaagac cttcattatt ttctccaatt gcttaccttt acttcttatc
     7741 ataagccgta tcgcagtata cgcacctgta tattcctttt tccctgtcag caagtctgaa
     7801 tttatgaact atttcctgct ctattgatgt tatgcatctc gggtttttac atttaactac
     7861 attttcaact tcctgaggca attctaaatt aattttgtct attataacag aattgtctat
     7921 tacattgatt gttatgtttg ggtcaataaa accaagcacc ttaaggtcca tatccagatt
     7981 gttttctact tttattatat ctttttttcc gtatttcttg ctttttgcat ttttaattat
     8041 ggcaacactg caatccatct tatcaagatt caaataatga tatatttcca ttgccttgcc
     8101 tgccttaata tggtcaatta ccaatccttt ttctacactg tctatattaa gcattattcc
     8161 acccccaata gtttcaatat taaagccatt cttacataca taccgcattt agcctgtata
     8221 aaatatgctg ctctcggatc attgtcaact tctaccgata tttcattgac tcttggaagg
     8281 ggatgcataa gaatcatatc tttttttgca tttgacattt ttttgttgtc tacaatatag
     8341 ctgtccttta atcttatata gtccgcttca ttgaaaaatc tttctttttg aactcttgtc
     8401 atataaagaa catccaactc gccaataaca ttttctagtc tttccacttc cataaactca
     8461 ataccgtttt tcattaatat ttcttctctg atataatccg gcactctcaa ttcatccggt
     8521 gatataagaa taaattttat attttcgtat cttgataatg cttttatcaa ggaatgaacg
     8581 gtacgtccga atttcaagtc accacaaaga ccaatcgtaa aattatttat tttacctttt
     8641 aatgtttgaa tggtaagcag gtctgtcaat gtttgagtcg gatgctgatg acccccgtct
     8701 cctgcattta ttacaggaat atttgctttc attgcagcta ccatgggagc accctctttt
     8761 ggatgtcgca ttgctgctat atctgcataa catgcaactg tccttatagt atcagcaaca
     8821 ctttctccct ttgcagcaga gcttgattca gcagatgaaa aacccataac acttcctccg
     8881 agccttaaca ttgccgattc aaagctcagc cttgttcttg tgcttggttc gtaaaataaa
     8941 gtcgccagta cttttccctt acacttatct gcataggctg caggatcgtg cataattcta
     9001 tctgccagat ttaagatatc gtccagctct gcaagtgata aatccattgg ttcgattaag
     9061 cttcttcctt ttaacatttt tcctcctttt tagcctctca ggactaattt aaagtgtaca
     9121 ttaattcttt ctccagtcac tataccttta ataaacgggc tataaaaaat cttccttgcg
     9181 cacagcaaag gaagacctaa ttaataagct catattttcc ttcccagcct ctctgtgcca
     9241 gattaaaggt tattgacttc aactaatatt attatatttt tacctgactg tcaatatttt
     9301 tttactgccg gtcagaagtt tcattttcaa cttgatatat aatttttgtt atccttcttt
     9361 ctacttcgct tc
//
LOCUS       DAOSOW010000015         9323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_7334_length_9323_cov_3.205330, whole genome shotgun sequence.
ACCESSION   DAOSOW010000015 DAOSOW010000000
VERSION     DAOSOW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7334_length_9323_cov_3.205330"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PLD67_00930"
     CDS             complement(<1..453)
                     /locus_tag="PLD67_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOT21120.1"
                     /translation="MEINAYNSSYKVNNINTANDLKIQNNNEGLNMQDFLNLLVAQIT
                     NQDAMNPMDNTEFIAQMAQFSTLQAMTDLTEMALQGQATSLIGKNVVVADYNGNGGLT
                     IDEGIVQRVTLHGGKTKLYVNDMEYEYSNIMEIREAEKAEENSLTEMLG"
     gene            complement(443..1522)
                     /locus_tag="PLD67_00935"
     CDS             complement(443..1522)
                     /locus_tag="PLD67_00935"
                     /inference="COORDINATES: protein motif:HMM:NF021032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HOT21121.1"
                     /translation="MNVNSLTISQLPGKVIRSDYSNSVNKNMFENMLNNAMAENQNMM
                     ELLGKNFGSILAANSIEIEDYSIEHENKDETQKQNFYENIYGIADIFFFRPNAAIEEV
                     TAENKITNVDYNFIDAKLQNGTDITAGSALKNLVSEIEIQRDLGISNDIKSSNEAQSM
                     KTILELGKKQKNEPAYLEMPDCIDLKNKIITISDGSTEIKSQVLSQVKDKIIIMTENS
                     QDSNGIKTITMELRPQALGKVDIKMSYENNKLTVEIKASNEETQKILSSNTDELKELL
                     NKTSETDVKIIVKPYEPKNQNILNYQDNRQGSENFYQDNEQNNQGRQRNKYYYDNDIK
                     TKEEDVFSELIDLNYSRIKEGMHGN"
     gene            complement(1713..2165)
                     /locus_tag="PLD67_00940"
     CDS             complement(1713..2165)
                     /locus_tag="PLD67_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21122.1"
                     /translation="MKKFVFSLQKVLELKNQLLENMKIELTNLNRECEKIEIFIKCLK
                     IKFAEIENEYVEKSYKSISAGEMSYYKMLSESILVQIENKEIEKAIVLKKIADKRQEI
                     ISMNTEISSLEKLKEKELEKHSKECLKKEELFIEEFVSNKTMTNEYAI"
     gene            complement(2209..3516)
                     /gene="fliI"
                     /locus_tag="PLD67_00945"
     CDS             complement(2209..3516)
                     /gene="fliI"
                     /locus_tag="PLD67_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOT21123.1"
                     /translation="MLVNEKFNDIRETLIKEDFYTHIGKVKRISGLMIEAHGGKYKIG
                     EICEIKTASNKTIRAEVVGFNDDRVLLMPYEDIKGIGLGNLVVSTNNKLKIPVGDFLI
                     GRIIDATGQAIDGGKKFISQEYCYVDNNYINPLSRPRISEPLSFGVKAIDGLLTIGKG
                     QRMGIFSGSGVGKSTLLGMVAKNVKADINVIALVGERGREVREFIEKDLGEEGLRRSV
                     LVIATSDQPAMLRVKCALTATTIAEYFKDKGKDVLLMMDSLTRFAMAQREIGLAAGEP
                     PVSRGYTPSIYAELPKLLERSGNFQEGSITGVYTVLVEGDDTNEPISDTVRGILDGHI
                     VLNRKLANSNHYPAIDINASISRLMDDIATEEHNNKAKKLRDILAEYYSNYDLISIGA
                     YKKGSNVKLDEAISKIEEVNNFLKQGVNEKYNFDEIINLMDKI"
     gene            complement(3506..4261)
                     /locus_tag="PLD67_00950"
     CDS             complement(3506..4261)
                     /locus_tag="PLD67_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HOT21124.1"
                     /translation="MSNIIKAEYVICDIDKQKNNHIHRQVSTYREDLFRIYNQREILL
                     KEAEEKAAEIVSTAIENAQLEITECKKNAYEDGYNAGMEAGRNKGYKEGYDTGKSKIS
                     EILLEQNKEKLNEIAEMIKTIESEKLKIISRYEEELIKLSIEIAEKIIRHEIDTKDDV
                     VSGIIKNAIKDYRNVDWIKIYLSDKDDAEIIQADKELISELEKISKDIKIEVMDELQK
                     GGAIIETADGIVEASIDTQLKNLKEMVLNKNAG"
     gene            complement(4254..5258)
                     /gene="fliG"
                     /locus_tag="PLD67_00955"
     CDS             complement(4254..5258)
                     /gene="fliG"
                     /locus_tag="PLD67_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOT21125.1"
                     /translation="MAERLTGKQKAAAIIISLGDEDASRIYKLLKEDEIEQLTYEISR
                     LQHLSPQTMESTLKDFYELCLTQKVITEGGLEYAKNVLEKAFGTQVATSLLERVTKTL
                     RNKSFDFIRQADYKNLLTIIQNEHPQTIALILSYARADQAAAVIAELPKEKRIDVVER
                     IAKMDRTSPEIVKYVEKELLKKFNSMVSIDYTEIGGINYVADIMNNMDRGNEKYIFDE
                     LNKKDAKLSDEIRKKMFIFEDIVTLDSLSIQRFLREVDSKDLVLSIKGSNKDVSDVLF
                     NNMSTKMADTIRSELEYTHNVRLRDVEEAQQRIVSVIRRLEEEGEVIIAKSGKDDIIV
                     "
     gene            complement(5260..6822)
                     /gene="fliF"
                     /locus_tag="PLD67_00960"
     CDS             complement(5260..6822)
                     /gene="fliF"
                     /locus_tag="PLD67_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOT21126.1"
                     /translation="MSEQLKNIWKNVTEFWGKLNKRKKSLIIGGLLVLVIGALIITFI
                     LNNKDYVVLFRNMDEAESVEVMKQLQESKIDYKYQTDGTILIPKEQESLLRMQLAQAG
                     HPRTGINYDIFTENIDFMTTDYEKRQYEIFQLQERLQSSIETIDGVKEAIVTINLPQD
                     KSFAWETDKKESSASVKINLQNGYSLSPSQTNGIMQLLVKSVKGLKEENVAIIDTNGN
                     SLNISNEMLQTNTIKLKLSIEREFERETEEDVTEFLSRIYGPENVKVSAKCTINFDKK
                     ISEILQYLPDEETRLGVPGDTHSEKEITGPGETTGGIAGTETNAEVPTYPGVVVSGDD
                     IYFRDSNTINYLVSQLKEQIEHNPGTIEKLTVAVVINKANMSDKEAGEINQLIAFAAG
                     IGTEDIALSNMVFYDLEEPAVPVAATGIFERIQLKKLLIYGGIALIAISILLIILILV
                     LRKRKKKKALDNAAITSDADSSEYSWADIQDGIKVQETEEQAIKKQLKDFTKTSPEIV
                     SQLIRTWLKGDE"
     gene            complement(6849..7145)
                     /gene="fliE"
                     /locus_tag="PLD67_00965"
     CDS             complement(6849..7145)
                     /gene="fliE"
                     /locus_tag="PLD67_00965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOT21127.1"
                     /translation="MFIENIKPINASQINELNKPKEKISELAFKSIFDTAIENYKNAE
                     AEVDKDIYKLSIGETDDLHNLMINVEKAQISLDLVIQLRNKALEAYNEIMRMGV"
     gene            complement(7148..7579)
                     /gene="flgC"
                     /locus_tag="PLD67_00970"
     CDS             complement(7148..7579)
                     /gene="flgC"
                     /locus_tag="PLD67_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008720497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOT21128.1"
                     /translation="MSFLKTLNISGSGLTAQRARMDVISENIANIDTTRTEEGGPYRR
                     KMVVLKTSGDFKNMMVKNLNQYEPGGVEVAEIMEDNSEFKAVYNPEHPDADENGYVNM
                     PNVDSLKETIDIMEAFRAYQANITALNTVKQMAIKALEIGR"
     gene            complement(7648..8031)
                     /gene="flgB"
                     /locus_tag="PLD67_00975"
     CDS             complement(7648..8031)
                     /gene="flgB"
                     /locus_tag="PLD67_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOT21129.1"
                     /translation="MRVNVNLNLLKKSLDGLWLRQQTTMENIANYSTPGYKSKTVDFE
                     TILSANLKNTYGKTTSEINEQILSGNIIIKENDNLSMRLDGNNVDLEKENLQLAKTQI
                     QYMYTVAEINSYFSKLKSVISEGKK"
     regulatory      complement(8157..8239)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(8537..8773)
                     /locus_tag="PLD67_00980"
     CDS             complement(8537..8773)
                     /locus_tag="PLD67_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HOT21130.1"
                     /translation="MLVIKRKVSESILIGDDIEIIISEISQDKVKIAISAPQDIKITR
                     KELAETCEFNKIASEKINEISLNDIKNKLKAIKN"
     gene            complement(8767..9219)
                     /gene="fliW"
                     /locus_tag="PLD67_00985"
     CDS             complement(8767..9219)
                     /gene="fliW"
                     /locus_tag="PLD67_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HOT21131.1"
                     /translation="MSEKMKIKTRDFGEILVGEEDIINFTTGIYGFDDYKKYIILKDS
                     PEDDIMFLQSLDNMELSFVLIDPYAIFIDYNPILNEDDLCELKSTGTAELKYLVIAII
                     KDDIKESVVNLKSPIAINPNNRTAKQVILQNEYPLRYNIVSTEEGNKC"
BASE COUNT         2428 a         1810 c         1284 g         3801 t
ORIGIN      
        1 tcctaacatt tctgtcaatg aattttcctc tgctttttca gcttctctaa tttccattat
       61 attggaatac tcatattcca tatcatttac atacagcttt gtttttccgc cgtgcagcgt
      121 aactctttga actattcctt catcaattgt taatccgccg ttaccgttat agtcggcaac
      181 cacaacattt ttccctatga gtgatgtagc ttgtccctgc aatgccattt ccgtcaggtc
      241 cgtcatagcc tgaagcgttg aaaactgcgc catctgtgct ataaattccg tgttgtccat
      301 tggattcata gcatcctgat tagtaatctg agctacaagt aaattcaaaa aatcctgcat
      361 atttaaacct tcattattat tttgaatttt taaatcattt gctgtattaa tgttgtttac
      421 tttgtagctt gagttgtaag cgttaatttc catgcatacc ctccttaatc ctggaataat
      481 ttaagtcaat aagctctgaa aatacatctt cttcttttgt cttaatatcg ttatcatagt
      541 agtatttgtt gcgctgcctg ccttgattat tttgttcatt gtcttgatag aaattttcgc
      601 tgccttgccg gttgtcttga taatttaaaa tattctgatt ttttggctca taaggcttca
      661 cgatgatttt tacatctgtt tctgatgttt tatttaacaa ctcctttagc tcatctgtat
      721 ttgaagatag tattttttga gtttcttcat ttgatgcttt gatttctacc gttaacttat
      781 tattttcata gctcatttta atatctacct tgcctagagc ttgaggtctg agctccatgg
      841 taattgtttt tatgccattt gaatcctgac tgttttctgt cataataatt attttgtctt
      901 ttacttgaga tagtacttgt gacttaattt ctgttgagcc gtcactaata gttattattt
      961 tgttttttaa gtcaatacaa tccggcatct ctaaatatgc tggttcattt ttttgttttt
     1021 tgccaagctc aagtatagtc ttcatacttt gtgcttcatt tgagcttttg atgtcattgc
     1081 tgattcctag atctctttgg atttcaatct ctgacaccaa atttttaagt gctgagccgg
     1141 cagtgatatc agtgccgttt tgaagcttag catcaataaa attataatcc acatttgtta
     1201 ttttattttc ggctgtaact tcttcaattg ctgcattggg tctgaaaaag aaaatatctg
     1261 caataccata tatattttca taaaaatttt gtttttgtgt ttcatctttg ttttcatgct
     1321 caatactata atcttcaatt tcaatactgt ttgcagccag aatgcttccg aagttttttc
     1381 ctagcaactc catcatattt tgattttctg ccattgcatt attgagcata ttttcaaaca
     1441 tatttttatt cacgctgttt gaataatcac ttcttataac cttaccgggc aattggctta
     1501 tcgttaaact gtttacattc aaattctcac ctcctttcaa tataattcag cagttgggga
     1561 cacacacgag gagtgtcccc gtacttacat tcatatttcc ctcctttcta ttgatattca
     1621 atggtttcac ctgtagttgt tatcccgggc tcagactttg agatccttcg ctgcgctcag
     1681 gatgacgcgg gctgcgccat caggtctcca aattatatag catattcatt tgtcatggtt
     1741 ttatttgaaa caaattcctc tatgaaaagt tcttcctttt ttaaacattc cttgctgtgt
     1801 ttttctaatt ccttttcttt tagtttttct aaacttgaaa tttctgtatt catactgatt
     1861 atttcttgtc ttttgtctgc tattttcttt aatactattg ctttttcaat ttccttattt
     1921 tcaatctgta ctaatatgct ttcactgagc attttataat aagacatttc accagctgat
     1981 attgatttat atgatttttc tacatattca ttttctatct ctgcaaactt tatttttaag
     2041 catttaataa atatttcaat tttctcacat tctcgattta agtttgtgag ttcgattttc
     2101 atattttcca acagctgatt ttttaattct aaaacttttt gaagtgaaaa aacaaatttc
     2161 ttcatttcct gcctcgctta aaatggtatt tttgtaatat tgaaagtatc aaattttatc
     2221 catcaaatta attatttcat cgaaattata tttttcattt acaccttgtt ttaaaaagtt
     2281 atttacttcc tcaattttac taattgcttc atccaattta acattgcttc cttttttgta
     2341 ggcacctatg gaaattaaat cataattaga gtagtattca gctaatatat ctctgagttt
     2401 tttggcctta ttgttgtgct cttcggttgc aatatcatcc ataagtcttg atatacttgc
     2461 atttatgtcg attgccggat aatgatttga attagccaat tttctgttta gtactatatg
     2521 tccgtcaagt attcctctga cggtatccga aataggctca ttggtgtcat ctccttcaac
     2581 aaggactgta taaacacccg taattgaacc ttcttggaag tttccgctcc tttctaacag
     2641 ctttggcagc tcggcataaa ttgacggggt ataccctctt gatacaggcg gttcccctgc
     2701 agccaggcct atttctcttt gagccattgc aaatcttgtc aaggaatcca tcattaataa
     2761 aacatctttg cctttatctt tgaaatattc tgctatggtt gttgctgtta atgcacattt
     2821 tactctaagc atggcaggtt ggtcggatgt tgctattacc aatacggacc ttcttaatcc
     2881 ttcttctcct aagtcttttt caatgaattc tcgtacttct cttcctcttt ctccaaccaa
     2941 tgcgatgaca tttatatccg cttttacatt ttttgcaacc atcccgagaa gggtgctttt
     3001 cccaacgccg cttcccgaaa atatgcccat acgctgtcct tttccgatgg ttaaaagtcc
     3061 gtcaattgct tttactccaa agcttaaagg ctcgcttatc cgaggtcttg acaaaggatt
     3121 tatataatta ttatcaacgt agcaatattc ctgactaata aattttttgc cgccatcaat
     3181 agcttgtcct gttgcatcta taattctgcc tatcaagaaa tctcccacgg gtatttttag
     3241 cttgttgttt gttgatacaa cgagattccc gagacctatg cccttaatat cctcataggg
     3301 catcagcagc actctgtcat cattgaagcc cactacttct gctctaattg ttttgtttga
     3361 cgcagttttt atttcgcata tttctccgat tttgtacttg ccgccgtgtg cttctatcat
     3421 caatcccgat attcttttga cttttcctat atgtgtatag aaatcttctt ttattaatgt
     3481 ttctcgaata tcattaaatt tttcattaac cagcattttt attaagtacc atttctttaa
     3541 gattttttaa ctgggtatca atacttgctt cgactattcc atctgctgtc tcgattattg
     3601 caccgccttt ttgcaattca tccataactt ctatttttat gtctttagat attttctcaa
     3661 gttcacttat taattcttta tctgcttgaa tgatttctgc atcgtcttta tccgaaagat
     3721 aaatctttat ccaatctaca ttgcggtaat cttttattgc atttttaatt attcccgata
     3781 caacatcatc ttttgtatca atttcatgcc tgataatctt ttctgctatt tctatagaga
     3841 gtttgattaa ttcttcttca tatcttgaaa ttatttttag tttttcggat tctatggttt
     3901 taatcatttc agctatttca tttaattttt ctttgttttg ctctaagagt atttctgata
     3961 tcttagattt tcccgtatca tatccttctt tgtagccttt gtttctgcct gcttccattc
     4021 ctgcattata gccgtcttcg taagcatttt ttttacattc tgttatttcc aattgggcat
     4081 tttctattgc agtactgaca atttcggcag ccttttcttc ggcttctttt agaagaattt
     4141 ctctttgatt ataaattcta aacaaatctt ccctgtaagt actcacttgc ctgtgtatat
     4201 gattgttttt ctgtttgtct atgtcacaaa tcacgtattc ggctttaatt atattagaca
     4261 ataatatcat ccttcccgct cttagcaatt ataacttctc cttcttcctc aaggcgtctg
     4321 attactgata caattctttg ctgagcttct tctacatctc ttaagcgtac attgtgagtg
     4381 tattccagtt ctgaccttat tgtatctgcc atcttagtgg acatattatt gaataatacg
     4441 tccgaaacat ctttatttga acctttaatt gacaatacca agtctttgct gtcaacttca
     4501 cgaaggaatc tttgaatact caatgaatct aaggttacaa tatcctcgaa tatgaacatc
     4561 ttctttctga tttcatcact tagcttagca tcctttttat tgagttcatc aaatatatac
     4621 ttttcgtttc ctctgtccat attattcata atatcagcaa catagtttat gccgccgatt
     4681 tccgtataat caatagatac cattgaattg aatttcttca ataattcttt ttccacatac
     4741 tttacgattt ccggtgatgt acggtccatt tttgctattc tttctaccac atctattctt
     4801 ttttcctttg gcaattctgc aataactgcc gctgcttggt ctgcccttgc ataagatagt
     4861 attaaagcta ttgtttgagg atgctcgttt tgtataatgg ttaataaatt tttgtaatca
     4921 gcttgtctaa taaaatcaaa cgacttattg cgcagtgtct ttgttactct ttctaaaaga
     4981 cttgttgcta cttgagtgcc aaatgctttt tcaagtacat tttttgcata ttctaaaccg
     5041 ccttctgtta ttactttttg cgtaagacat agctcgtaaa aatctttgag cgttgattcc
     5101 attgtttggg gactaaggtg ctgcagcctg gaaatttcat atgttaattg ctcgatttcg
     5161 tcttccttta acaatttata aattcttgat gcatcttcgt caccgagaga tataattatt
     5221 gccgcagctt tttgtttacc tgttagtctt tccgccatat tattcatccc cctttaacca
     5281 ggttctgatt agctgtgata cgatttccgg actggttttc gtaaagtctt taagttgttt
     5341 cttaatcgct tgctcctctg tttcttgaac cttgattcca tcttgtatat cagcccatga
     5401 atactcgctt gaatctgcat ctgatgttat ggctgcatta tctaaggcct ttttcttttt
     5461 tctttttctt aacacaagaa ttaatattat taataatatt gaaattgcaa ttaaggctat
     5521 accgccgtaa ataagcagct tctttaattg aatcctttca aagatacctg ttgcagccac
     5581 cggtactgcc ggttcttcta agtcataaaa caccatattg ctcaatgcaa tatcttcggt
     5641 tcctatacct gcagcaaatg ctatgagttg attaatttct cctgcttctt tatcgctcat
     5701 gtttgctttg ttaattacta ctgctaccgt cagcttctca attgttcccg gattgtgctc
     5761 aatttgctcc tttaactggc ttaccaggta attaattgtg tttgaatccc tgaaatatat
     5821 atcatctccg ctgactacca caccgggata agttggtact tctgcattag tttcagttcc
     5881 tgcaatcccc cctgttgttt ctccgggacc agtaatttct ttttcgctat gagtatcgcc
     5941 gggtacaccc agtcttgttt cctcgtccgg taaatactgc aatatttcgc tgattttttt
     6001 gtcaaaattg atagtacatt ttgccgaaac cttgacattt tcaggtccgt atattcttga
     6061 aagaaattct gttacatctt cttcagtttc tctttcaaat tctctttcta tactaagttt
     6121 taattttatt gtatttgttt ggagcatttc attgctaata ttcaaactat tcccgttggt
     6181 atcaattatt gcaacatttt cttcctttaa tcctttaacg cttttaacca gcaactgcat
     6241 tataccgttt gtctgggaag gagataggga atatccgttt tgcaaattta ttttaacaga
     6301 ggcagaagat tcctttttat cggtttccca tgcaaaactc ttgtcttgcg gtaaatttat
     6361 agttacaatt gcttccttaa caccgtcaat agtttctatt gagctttgca gtctttcttg
     6421 taactgaaaa atttcatatt gcctcttttc ataatctgtt gtcatgaaat caatattttc
     6481 ggtaaagata tcgtaattta taccggttct gggatggccg gcttgagcta attgcattct
     6541 taataagctt tcttgttctt taggtatcaa tatggttcca tctgtttgat atttatagtc
     6601 aattttgctt tcttgcagct gtttcataac ttcaaccgat tcagcttcat ccatatttcg
     6661 aaataaaact acataatctt tgttattcag tataaaggtt attattagtg caccaattac
     6721 caatacaaga agtccgccta ttataaggct ttttttcctt ttatttagtt ttccccaaaa
     6781 ctcagtaaca tttttccata tgttttttaa ttgttctgac atttatagct cctaagatag
     6841 tatgagacct aaacccccat acgcattatt tcattgtaag cttctaaagc tttatttcta
     6901 agttgtatta ccaggtctaa agaaatttgt gctttttcaa catttatcat tagattgtgc
     6961 aaatcatccg tttcacctat tgataatttg taaatgtctt tatctacttc tgcttctgca
     7021 ttcttataat tttcaattgc agtatcaaat attgatttga atgcgagttc gcttattttc
     7081 tctttcggct tatttaattc attaatttga gatgcattta tcggcttaat attttcaata
     7141 aacataatta cctcccgatt tctaatgctt taattgccat ttgcttaacc gtgttaagag
     7201 cagttatgtt agcttgatat gctctgaatg cttccattat atctatcgtt tcttttaatg
     7261 aatcaacatt aggcatatta acatatccgt tttcatccgc atcgggatgt tcgggattgt
     7321 atactgcttt gaactcactg ttatcttcca taatttcagc aacttccact ccacccggct
     7381 catactgatt taaatttttc accatcatat ttttaaagtc gccggaagtt tttaatacaa
     7441 ccatttttct tctgtaaggt cctccttctt cagtccttgt agtatcaatg tttgctatat
     7501 tttccgaaat tacatccatc cttgcccttt gcgcagttaa accagaccca cttatattga
     7561 gcgtttttaa aaaagacata ttaccctcct tgaaatcgtt atcttatgta tattatcatt
     7621 gtgtatctca tgctaagtct attaagttta ttttttccct tcggatatta cagattttaa
     7681 tttggaaaaa taagaattga tttctgcgac agtatacata tactggattt gcgttttagc
     7741 cagctgcaaa ttttcttttt ctaaatccac attgttgccg tctaaacgca tggataggtt
     7801 atcattttcc ttaatgataa tattaccgga taaaatttgc tcgtttattt cagatgtagt
     7861 ttttccgtat gtatttttta gattagccga tagaatggtt tcaaaatcaa ctgtcttgct
     7921 tttgtatccg ggagttgaat agttggctat gttttccatt gttgtttgtt gtctaagcca
     7981 taaaccatcc aaacttttct ttaacaaatt taaatttaca ttgactctca cttcttgccc
     8041 ctccaacaaa tttatgaaat aaagagtgat ttgaaaatat ccaaaaaaga aatgtataat
     8101 tattgacagt taccaaaata actatgaatt atacatttct tttgatataa atcatacatg
     8161 gcaaccggct accatagaca agtctttagg tccaacagct ttgcgtcact acctttcgat
     8221 agttttgccc tttacacata agtattaagt tgtataaatt tgtaaagtaa tcccattata
     8281 aatgaggtta aaacaaaatg caagcctttt ttacaaaatt ttacaaaaat ctttatacat
     8341 tttcttgatt tattattcgt ataatgcctt tatatttata catcctcatg cgttattttc
     8401 tggtgcatta cagcgaataa aaaaatgggt attgcaaaaa cacccataat tgttataaat
     8461 attatttttt ttcgactagc aaaattagca taattataaa ttatgcatca aaatatcaaa
     8521 aaaatattac atttttttaa ttttttattg cttttaattt gttttttata tcatttaagc
     8581 ttatttcatt tattttttca gaagcaattt tattaaattc acaagtttca gccaattctt
     8641 ttctggtaat ttttatgtct tgaggagcac tgatagcaat tttaacttta tcttgactta
     8701 tttctgaaat aattatctca atatcatcac cgataagtat ggattctgat acctttcttt
     8761 ttatcactaa catttattac cctcctcagt tgatactata ttgtatctga gtggatattc
     8821 attttgcagt ataacctgct tagctgttct gttattagga ttaattgcaa tcgggctttt
     8881 taaattaacc acggattctt taatatcgtc ttttataatt gcaattacca aatacttcaa
     8941 ttctgccgtg ccggttgatt tcagctcaca taaatcatct tcgtttaaaa tcggattata
     9001 atctataaaa atagcatacg gatcaatcaa cacaaaagat agttccatat tatcaagtga
     9061 ttgaagaaac attatatcat cctcgggact atcctttaat ataatatatt ttttgtaatc
     9121 atcaaatccg tatatgcctg tggtgaaatt tattatatct tcttcaccaa ccaaaatttc
     9181 tccaaaatct cttgttttaa ttttcatttt ttcagacaag ggtatctatc tccttataat
     9241 taggattcgc actgggcggc acaaaaatcg gggtacctac atattttatt tctacgcttg
     9301 ggtattcact gataataaat tct
//
LOCUS       DAOSOW010000016         9177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_7503_length_9177_cov_3.530037, whole genome shotgun sequence.
ACCESSION   DAOSOW010000016 DAOSOW010000000
VERSION     DAOSOW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9177
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7503_length_9177_cov_3.530037"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2822
                     /locus_tag="PLD67_00990"
     CDS             <1..2822
                     /locus_tag="PLD67_00990"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HOT21132.1"
                     /translation="KVYEIVAGFLNKNAMAIYENIENYISKLRIKDILPPIDLNAGKI
                     LSSEQAQKYIKNKSQNYVKAVLSKQLNELLENNSDIFIKSTADYLKVKFIDNEISIKN
                     FLSKKIKDFKIDNNYLKSKELTNFIRLELYDKYKEEASKLKDFDLSLALDKLNSIENI
                     SKNSSETFRKYIISNTDIILKGSIKGVVTDNLNKLSDDELVKFANDFVGRELKPIMFF
                     GGVLGVIAGLILAAFQNSPLDPANVNIANMATYAFVGFITNVIAINMIFKPYREIKPL
                     SKIPFFRNFSLGYIIKNQKNFAKSTAHYIDTNLLSKKSINELFEKHKDSIKQSFIKNI
                     AENDYSTLSSLLVKNRESTIRGTYNFIKNNILKKIIVFSDYLYGRISKTSLSTVLTGK
                     NINSISNFACECLKTNNIKSRIFSVIGSDNKLNKFFSIGFLVKLQNNRFAKLYGKSIN
                     YLKPDTIKNKILQYNYKYKLHTKRKIEEVFSINEVSRFSGKVNKAVLSEGFKNNVTLA
                     AVSLFNKLFDRNKTFGDLFDGRLKIYIDKNLPRILKNITQKIKNSLSESKKFVSTALR
                     AEFKNQLGFFESGLYAIMDGDTIIDHIVGKIMTEKLPLFMDMKEDEINKIISELINDR
                     FYKAKVEVLYANLNKLQINEVVENYLIANKEKLEAKINKAIVELYNKIKYKDLDGILD
                     YFNMSDLESFISSYDSEINAFANAMHLYLLNNKDDFTNEASSYIGSVAEEFMNLKFND
                     IFNYETQDEIDIIIGNATNILCKKEVIKKTIESFIGTYKKYCNNIHLDFYIDEDEFTN
                     SIQIFAQNLLMKDETEKTVKQILYSVINEAAQSNFSFIDSKSKEHIVNIFVDSSIEGL
                     RRNLDDILMSVEFDVIAAEEIEKMEPEKIHLMFNSFGEKYFRRLMLYGFGGLVFGINM
                     YVGFALTGLKILSETFRMKYFADGE"
     gene            complement(3018..4253)
                     /locus_tag="PLD67_00995"
     CDS             complement(3018..4253)
                     /locus_tag="PLD67_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOT21133.1"
                     /translation="MKRKISLLIVLMLILSTLINVSATEEAVEGEEMAEAAVETLPVN
                     EPLMLSMEDAVRIATENSREMWKIDDGIAQVKDARKDAKDAKNLAEIIMSIPLSQIEA
                     TFGPDFDVTSNQVERILAKNDYYIKFADSQQAQLEKNREMLLAGIEIETKSLYYNVLV
                     AEQTIVINKLNLSKAEEQLRVANLKFNNGSATKAEVLNAEMAVQKAKTELDSAVDDYS
                     IAKLNLLNKLELPFDTEYILTDTNFTYVPTEEINLVEAIEKAKAERPEILKAENNLEL
                     QKIETHAYTAYYTSNLRQHKAAKEKLKDAELNVPQAYKDVELDVRKSYLNLIKAERAL
                     INMEKTFELAKEAARINQLLYDNGMATMLNVLEANTGMYQAEIGYYQLLAAYNINKLM
                     FDNSHLIGSISGAPEGSDF"
     gene            complement(4263..4853)
                     /locus_tag="PLD67_01000"
     CDS             complement(4263..4853)
                     /locus_tag="PLD67_01000"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT21134.1"
                     /translation="MREKNQKRQDILNSAMTLFRDKGFHNTKMEDIAIGAGVGKGTLY
                     EYFESKQDVFDEACIEYVDVIIENIEYVSNIDDTFKNKLLILFNGKLKMESEFADMSI
                     DNVLSSKNIISEKTIKTIIGKITEMYSLICKIIDQGKEEGVVKKNIQSEVLACLIVGT
                     LGEYSRLKLQNKEKEIKEDDVIFDLLYNGFAVKHQQ"
     gene            complement(4873..7950)
                     /locus_tag="PLD67_01005"
     CDS             complement(4873..7950)
                     /locus_tag="PLD67_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOT21135.1"
                     /translation="MLARFSVKKPVTITMMILIVIVIGVVSFSKLQIDLLPQMELPYV
                     MVQTTYQGAGPEEIENMISRPLEQTLSTVENIEDIISISNEGSSLLLMQFAFNTDLDE
                     IMLQIRERIDVIKSFLPDGTSTPLALKMDPNAIPIIQLAVSSKGDLYTTQKIAEDVIS
                     PRFERIEGTASASVSGGLEQEVEIMLKEETVKGYNLNSSYVAQILQAENLNMPGGTVK
                     KGNNELTVRTMGEFKSVEEIRNLIIPLMKGGTVRLKDIADVNLKNKEQSSITKLNGKE
                     VVQISVMKQSDANTVNVANDIEKEIERIKTEYPELDLVTVFNQADYINFAIDNLIGNT
                     TKGGILAILILLIFLRSFKTTLVIAISIPFSIITTFVILFFTDITLNMMSIGALALAV
                     GMLVDNSIVVLENIYRNRSLGMDRFTASVDGTNEVSMAVTASTLTTIAVFIPIVFTGG
                     LAAVLFKEFALTITIALLSSLVIALTLVPMLSSKLVSVKNLESEEEQEKKLGPLVVFY
                     KKILSYSLKHRLITILISIVLFVVSVIMVISVGAEFFPAGDEGMINVSVSLPAGSETQ
                     EIDAVLNEIQETITEIPEIEDVFTSAGGGNMYGMGGSGGSGSMIVLLRDFDERDKSAK
                     EISDEIRIMTKDIPGAEISVSESSTMMLGLTAGAISISIKGDDLDTLKAIGDDFKKII
                     EKVEGTREVTTSYEDGIPQVQIIADRGIASQYGLTTAQIGRAVINTLSGSNVTKYKIN
                     GDEIDVVLKGDNIYGESISYLEMLPVETPMGSNVPLSEVADIRIEKGPISILRENQVR
                     VLTVSGSVMGRDVQSASIEIEELLKEYEMPYGYSYILGGEIEQIQETFTDLFLVMLMA
                     IALVYMIIAAQFESLIQPLSIMFSVPLALSGGFIGLFITGLPLNVIGIIGLIILVGVV
                     VNNAIVLIDYINNRRRRGEDRNVAIMKAGPIRIRPIMMTALTTILGLVPMSLGIGEGA
                     EFTQSMGVVVIGGLSLSTVLTLVIVPVMYTIFDDISEFFKKKIKKDETTELIL"
     gene            complement(7953..9059)
                     /locus_tag="PLD67_01010"
     CDS             complement(7953..9059)
                     /locus_tag="PLD67_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT21136.1"
                     /translation="MKKVLTLLLITVFIFTGCTAGQETKVNEEEAYTAVEVETLMPMD
                     LYIENLMTGKVFADKDVFVIPLMAGKVERVNVKVGDKVEKDDVLFVMDKDDIEKQVNQ
                     AYAAYEAAKAGFEVSAAQIQSAKDNLERIKKLYEEGAVPETQYEQAKLAASDESLEAA
                     RMGVEQARVAYSNAASMLKNAEVKAPISGVISSVNIVEGEYATSSNPPVTIVDSDSVT
                     IEFGISASLVNKVKAGDKTLVEISSAGYKNEAVINSVSSSADIMTNLYTVSIVLENDG
                     AIKPGMFAKVYLKTDNIANALAVRAEAVKEREDKKYVFVAEDNSAIEKEVTTGLDTGA
                     YIEIKTGLSEGDKVIVKGQDYISHGSKIKVVRGE"
BASE COUNT         2911 a         1717 c         1347 g         3202 t
ORIGIN      
        1 ataaggttta tgaaattgtt gcaggctttt taaacaaaaa tgccatggct atttatgaaa
       61 atattgagaa ttatatttca aaacttcgaa taaaagatat tttgccaccg attgatttga
      121 atgcaggtaa aattttatct tcagaacagg cacaaaaata tataaaaaat aaatcacaaa
      181 attatgttaa agctgtgtta tccaagcaat taaatgaatt gcttgaaaat aattcagata
      241 tatttattaa aagtacagct gattatttaa aagtaaaatt tattgataac gaaatttcaa
      301 ttaagaactt tctatccaaa aaaataaagg attttaaaat agacaataat tatttaaaaa
      361 gcaaggagct tacaaatttt atccgtttag aattatatga taaatataag gaagaagcct
      421 ctaaattgaa agattttgat ttatctttag ctttggataa attaaattct atagaaaata
      481 tatcaaaaaa cagctcggaa acctttcgca aatacataat aagcaatacg gatatcattt
      541 taaagggctc tataaagggt gttgtgacag ataatttaaa taagctgagt gatgatgagc
      601 ttgttaaatt tgccaatgat tttgtaggtc gggagttaaa acccataatg ttttttggcg
      661 gcgtattggg agttattgca ggcttaattt tggctgcatt tcagaattca ccccttgacc
      721 ctgcaaatgt gaatatagcc aacatggcaa cctatgcttt tgtaggcttt attacaaatg
      781 taatagcaat aaatatgata ttcaagccct acagagaaat taagcctttg tctaaaattc
      841 cttttttccg caatttctcg ctaggctata taattaaaaa ccagaaaaac tttgcaaaga
      901 gtactgccca ttatattgac acaaacctat taagcaaaaa aagcataaat gaattgtttg
      961 aaaagcataa ggacagcatt aaacaaagct ttataaaaaa cattgccgaa aatgactatt
     1021 caacactaag cagcctgctt gttaaaaata gagaaagtac gataagggga acatataatt
     1081 ttattaaaaa caatatttta aaaaaaataa ttgtattcag cgactatttg tatggaagaa
     1141 tttctaaaac ttctctttca acggtgctta ccggcaagaa tattaacagt ataagcaatt
     1201 ttgcatgtga atgtttaaag acaaataata taaaaagcag aattttttca gttataggtt
     1261 cggataataa attaaacaaa tttttttcaa tcggtttttt agttaaactt caaaataata
     1321 gatttgcgaa attgtatggt aaatcaatta attatttgaa acctgataca attaaaaata
     1381 aaattttaca gtataactat aagtataagt tgcatacgaa aagaaaaatt gaagaagtat
     1441 tcagtataaa tgaagtatca agattttccg gaaaggttaa taaagctgtt ctttctgaag
     1501 gttttaagaa taatgttact ctggctgccg tcagtttatt taataaatta tttgacagga
     1561 ataaaacctt tggagattta tttgacggca gattaaaaat atatatagat aaaaatctgc
     1621 ctcgaattct taaaaatata acacaaaaaa taaaaaatag tctttcagaa agcaaaaaat
     1681 ttgtttcaac agcccttcgg gctgaattca aaaaccagct gggttttttt gaaagcggtc
     1741 tgtatgccat aatggacggc gatacaatta tcgaccatat tgtcggtaaa attatgacag
     1801 aaaaactacc tttgtttatg gatatgaaag aggatgaaat aaacaaaatt atatctgaat
     1861 taataaatga caggttttac aaagctaagg ttgaagtttt atatgcaaac ttaaataagc
     1921 ttcaaataaa cgaagttgtt gaaaactacc taattgcaaa taaagagaag cttgaagcta
     1981 aaataaataa agcaatagtt gaactataca ataaaataaa atacaaagac ttagacggta
     2041 ttttagatta ttttaacatg agtgacttgg aaagttttat aagctcatat gactctgaaa
     2101 ttaatgcatt tgcaaatgca atgcatttat acttattgaa caataaggat gactttacca
     2161 atgaagcttc ttcttatatc ggttctgtgg cagaagaatt tatgaatttg aaattcaatg
     2221 atatttttaa ttatgaaact caagatgaaa tagacataat tattggaaat gcaacaaaca
     2281 ttctttgtaa aaaggaagtt ataaagaaga ctatagaatc tttcatagga acatataaaa
     2341 aatactgtaa taatatccac ttggattttt atattgacga ggacgaattt acaaactcta
     2401 tacaaatttt tgcacaaaat ttattgatga aggatgaaac tgaaaaaaca gtaaaacaaa
     2461 tcctatattc tgttattaat gaagctgcac agtctaattt ttcttttatt gattctaagt
     2521 caaaggaaca tattgttaat atatttgttg actcaagcat tgaaggtctt agaagaaact
     2581 tggatgatat acttatgtcc gttgaatttg acgttattgc agcggaagaa atcgaaaaaa
     2641 tggagccgga gaaaattcat ctcatgttta attcatttgg agaaaaatat ttcagaaggc
     2701 ttatgcttta cggatttggt gggcttgttt ttggaataaa catgtatgtg ggatttgcat
     2761 tgacagggtt aaaaatattg tccgaaacat ttagaatgaa atacttcgct gacggcgaat
     2821 gacgaaaggg tcgctcgtaa gcgaccctat gtgatttaca taattttaaa tatgtaaacc
     2881 tatgactaca accccaaacc tttatattct caaaacttcc gaattttaga tttacataaa
     2941 tttatatatg taaacctttt gacaaaaccc cttttcccaa caatgactga aattttcata
     3001 aaaacgaaag agcgtcccta aaaatcggat ccttccggtg ccccgcttat actgccgatt
     3061 aaatgagagt tatcgaacat caatttgttt atattgtatg cagccaatag ctgataatat
     3121 ccaatttctg cctgatacat gcctgtattt gcctccaata cattcagcat agttgccatt
     3181 ccgttatcat acaaaagctg atttatcctc gctgcctctt ttgccaattc aaaagttttt
     3241 tccatgttaa ttagggctct ttctgcctta atcaagttca aatacgattt tcttacatca
     3301 agttcaacgt ccttgtatgc ttgcggtaca tttaactctg catccttgag tttttctttg
     3361 gcggctttgt gctgtcttaa gtttgaagta tagtatgcag tataagcatg cgtttctatt
     3421 ttttgaagtt ctaaattatt ttcagccttc agtatttcag gtctttctgc ttttgctttc
     3481 tctattgctt caactagatt aatttcttca gtcggtacat atgtaaaatt ggtatccgta
     3541 agtatatatt cagtatcaaa tggtagttcc agtttattta ataaatttaa ctttgcaatg
     3601 ctgtaatcgt ctactgcaga atccaattca gtcttagctt tctgaactgc catttcagca
     3661 tttaatactt ccgcctttgt agcagaaccg ttattaaatt ttaaatttgc aactcttaat
     3721 tgttcctctg ctttacttaa atttaacttg ttaattacaa ttgtttgctc ggcaaccagt
     3781 acattgtagt ataatgactt tgtttctatt tcaatgcctg ccaaaagcat ttctctgttt
     3841 ttctccaatt gtgcctgctg ggagtcggca aatttaatat aataatcatt ttttgccagt
     3901 attctttcaa cctgattact tgttacatca aagtccggac caaatgttgc ttcaatttga
     3961 gataatggaa tgctcattat tatctctgcc aagtttttag catccttagc atcttttctt
     4021 gcgtctttaa cctgagcaat tccatcatcg attttccaca tttctctgct gttttctgtt
     4081 gctattctga cagcatcttc cattgacagc attagaggct cattaaccgg cagtgtctct
     4141 actgcagctt ctgccatttc ttctccttcc actgcttctt ctgttgctga cacatttatc
     4201 aaggtactta atataagcat cagtactatt aataaagata tttttctttt catcctttcc
     4261 tcctattgtt gatgttttac agcaaatccg ttatacaata aatcaaaaat tacatcatct
     4321 tctttgattt ctttttcttt attttgtaat tttaatctcg agtattcacc caaagtccct
     4381 acaatcaaac atgccaaaac ttcagattga atattctttt tgacgacgcc ctcctccttg
     4441 ccttggtcta ttattttgca tattaaacta tacatttcag taatttttcc tattattgtc
     4501 ttgattgttt tttctgaaat aatgtttttg ctggataata cattgtctat gctcatatca
     4561 gcaaactctg attccatctt aagttttcca ttgaataaaa tcaataattt atttttaaat
     4621 gtatcatcta tattactgac atattctata ttttcaataa ttacatcaac atattcaatg
     4681 catgcttcat caaatacatc ttgtttgctt tcaaaatact cgtataaggt tccttttcca
     4741 acacctgcac ctattgcaat atcttccatc ttagtattat gaaacccttt atctctgaat
     4801 aatgtcatgg cggaatttaa tatatcctgc cgcttttgat ttttttcacg cattcctatt
     4861 tgcctcctat acttacagta tcaactcagt agtttcatct ttttttatct ttttcttaaa
     4921 aaattctgaa atatcatcga atatagtata cataacagga actatcacta aggttaagac
     4981 tgttgacaag gacaatcctc ctatcaccac aacacccatc gactgagtaa attcagcacc
     5041 ttcgcctata ccaagcgaca taggtactaa tcctaagatg gttgtcaatg cagtcatcat
     5101 tattggacgt attcttattg gacctgcctt cattatggca acattcctgt cttctcctct
     5161 tctccgcctg ttattgatgt aatcaattaa tactattgca ttattaacaa caacgcctac
     5221 caatattatc aaccctatta tccctattac attaagagga agccctgtaa taaacaggcc
     5281 tataaatcct ccggacaatg caagcggtac cgagaacata attgacaaag gctgaatcaa
     5341 tgattcaaac tgtgctgcaa taatcatata aaccaaggca attgccatca gcatcaccaa
     5401 gaataagtca gtaaaagttt cctgtatctg ctctatttct ccgccaagta tataactgta
     5461 gccgtaaggc atttcatatt ctttaagaag ttcttctatt tcaatggagg cagattgaac
     5521 atctcttccc ataacacttc ccgatacggt tagtactctt acctgatttt ctctaagtat
     5581 gcttattggt cctttttcta ttcttatatc ggcaacttca gataagggca cattgctgcc
     5641 cattggggtt tcaacaggaa gcatttccaa atatgaaata ctttcaccgt aaatattgtc
     5701 accttttaaa accacatcta tttcatcgcc gttaattttg tactttgtaa cattggaacc
     5761 ggataatgta ttaataacag ccctgcctat ctgagcggta gttaaaccgt attgggatgc
     5821 aattcctctg tctgctataa tttgaacttg aggaatcccg tcttcataac ttgtggtcac
     5881 ttctcttgtt ccttcaactt tttctattat ttttttaaag tcgtctccaa tagcttttaa
     5941 agtatctaaa tcatctcctt taatacttat gcttattgct cccgccgtca atcccagcat
     6001 cattgttgat gattcggaaa cacttatttc agctccgggg atatccttag tcataattct
     6061 tatttcatcg ctaatttctt ttgcactttt gtccctttca tcaaaatccc ttaataatac
     6121 aatcatagag ccgctgccgc cggagccgcc cattccatac atatttccac ctcctgcaga
     6181 ggtaaataca tcttcaattt caggaatctc tgtaattgtt tcttgtattt cattcaatac
     6241 agcatctatt tcctgcgttt cagagccggc aggaagacta acgctgacat taatcatacc
     6301 ttcgtcacct gcagggaaaa attcggctcc cacggatatt accattatta cgcttacaac
     6361 aaataatacg atacttatca atattgttat taaacgatgt tttaaactgt aacttaatat
     6421 ttttttatag aaaacaacca aaggaccgag ctttttctcc tgctcttctt ctgattcgag
     6481 gttttttacc gaaaccagtt ttgatgacag catcggtacc aatgtaagag caataaccaa
     6541 agaacttaat aaagcaattg ttatggtaag tgcaaattct ttaaataaaa ccgcggcaag
     6601 cccgccggta aacacaatcg gaataaaaac ggcaatagta gttaaggtgg aagctgtaac
     6661 tgccattgaa acttcgtttg ttccatctac tgatgcggta aatctgtcca tccctaagct
     6721 tctgtttctg tatatgtttt caagaacaac aatggaattg tcaaccagca ttcctaccgc
     6781 taaggctaag gctcctattg acatcatatt tagtgtaata tcggtaaaaa acagaataac
     6841 aaatgtggtt attatggaaa aaggtattga aatagctata accaatgttg ttttaaaact
     6901 tctcaagaaa atcaaaagta tcagtatagc taaaatacca ccctttgtcg tattacctat
     6961 caaattatct attgcaaaat taatataatc agcttggtta aaaacagtaa ctaaatccag
     7021 ctccggatat tctgttttaa ttctttcaat ttctttctct atgtcatttg caacattgac
     7081 tgtattggcg tcagattgct tcataaccga tatctggaca acttccttgc cgttcaattt
     7141 tgttattgat gattgctcct tgtttttcaa attaacatca gcaatatctt tcagcctgac
     7201 agtaccgcct ttcataagag gtattatcaa atttcttatt tcttcgacgg atttaaattc
     7261 gcccattgtc cttactgtta gctcattatt cccttttttg actgtccctc cgggcatatt
     7321 taaattctct gcctgaagta tttgtgcaac atatgaggaa ttcaaattat atcctttaac
     7381 cgtttcttct ttcagcatta tttctacttc ctgctctaat cctccggata cggaagcaga
     7441 agcagtgcct tctatacgct caaaacgtgg agatataaca tcctccgcaa tcttttgagt
     7501 tgtgtataaa tctcctttgc ttgaaacagc caattgaata attggaatag cattggggtc
     7561 catttttaaa gccaagggcg tagatgtgcc atccggcaaa aaactcttta taacatcaat
     7621 tctttccctt atttgaagca ttatttcatc caaatcagta ttaaatgcaa actgcattaa
     7681 taataatgaa cttccttcat tagatatgga aataatatcc tcaatatttt caaccgtgga
     7741 taaagtttgc tccaagggtc tagatatcat attttctatt tcttcaggtc ctgccccttg
     7801 ataggttgtt tgaaccatta cataaggcag ttccatttga ggaagcaagt ctatctgaag
     7861 cttgctaaag gatacaaccc ctataacaat cactattaat atcatcatgg tgattgttac
     7921 gggtttttta accgaaaatc ttgcaagcat tcttattcac cccttacaac ctttatttta
     7981 ctgccatgac ttatgtaatc ctgacctttc acaataactt tatctccctc tgagagacct
     8041 gttttaattt ctatgtatgc tcctgtgtca agtcctgttg taacttcttt ttcaattgcg
     8101 ctgttatcct ctgcaacaaa cacatatttt ttatcctcac gttctttaac ggcttctgct
     8161 cttacggcta aagcatttgc aatattatct gtttttagat aaaccttggc aaacataccg
     8221 ggtttaattg caccgtcatt ttccagcact atgcttacgg tatacagatt tgtcataata
     8281 tctgctgaag agcttacgct gtttattacg gcttcatttt tataacctgc tgaacttatt
     8341 tcaacaagag tcttatcgcc ggctttaacc ttatttacaa ggcttgcgga tattccaaac
     8401 tcaattgtaa cactgtcgga atcaactatt gttacgggag gattggatga tgttgcatat
     8461 tctccttcaa ctatgtttac actcgaaatt acaccggata tgggagcttt gacctcagca
     8521 tttttcaaca tcgatgctgc atttgaataa gctacccttg cttgttcaac acccattctt
     8581 gcagcttcta aagactcatc tgatgcagca agctttgctt gttcatactg agtttcggga
     8641 actgctccct cttcataaag cttttttatt ctctcgagat tatctttagc actttgtatt
     8701 tgagcagcac ttacttcaaa tcctgctttt gctgcttcat aagcagcata agcttgattc
     8761 acctgtttct ctatatcatc tttatccata acaaataaga catcatcttt ttctactttg
     8821 tcgcccactt ttacatttac acgttcaact tttccagcca ttaaaggaat tacaaaaacg
     8881 tccttatctg caaatacttt tccagtcata aggttttcta tgtataaatc cataggcatt
     8941 agtgtttcta cttctacagc agtataagcc tcttcttcat ttacttttgt ttcctggcct
     9001 gctgtgcagc ccgtaaaaat aaatacagtg attaataata aagtcaatac ctttttcaaa
     9061 ttgccctccg aaatttcgac cgaccggtca gttttaatga aattatatca ccttcaaaat
     9121 ttactgtcaa ttaatataat taagagacca aaaatcatta gtggtgccca ttagcgg
//
LOCUS       DAOSOW010000017         8634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_8135_length_8634_cov_3.856860, whole genome shotgun sequence.
ACCESSION   DAOSOW010000017 DAOSOW010000000
VERSION     DAOSOW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8135_length_8634_cov_3.856860"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLD67_01015"
     CDS             complement(<1..260)
                     /locus_tag="PLD67_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21137.1"
                     /translation="MAFLRSAINFIDMTERSIMNTNKNILRLVFFPLLILYYETILKA
                     FTYSKIFNIGYVYMCLFSIPLGLLFYLLSTGFNEKTNKIIFFS"
     gene            complement(314..1225)
                     /locus_tag="PLD67_01020"
     CDS             complement(314..1225)
                     /locus_tag="PLD67_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOT21138.1"
                     /translation="MKIIIHKKYLDIVSEKFPDIEFYTEIDECPEAEALIGEPMDFVP
                     EKLDKMPNIRWLQSFRAGYDTLDLDYMKKRNITFCNGKDIYSIPIAEDVVCKILMHNT
                     NALKFLRSQREHKWEPRQKRTEFHGQTVGIIGTGSIATEIAKRLQGFGVKIIGYKRTP
                     VSAMAYFDEILSGKKGLEYLMSNSDYIIVTVDLNKDTYHMINKDTLKFMKESASIINI
                     ARGSIINQKDLTEALKNKSISYAGLDVFEVEPLPEDDELWDLENVYITPHASGIVKEN
                     KKRLAELIVANIQRFIEHEKLANVVVK"
     gene            complement(1331..2191)
                     /locus_tag="PLD67_01025"
     CDS             complement(1331..2191)
                     /locus_tag="PLD67_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT21139.1"
                     /translation="MKYKEIISEIKSVRLYKKEEVDSKLLKELKQYYEQGKRLVDDIE
                     VGVIRRSRDEVFDKLKDSAGYNNRMIEAPHYLIFLSEEKDHYIENAGYRAQDIMLKAW
                     SLGIASCWITFKDGEEIKKKLGIDSDKKLVALIALGYEDPQNKVLYEKGSKYNTSSNV
                     KLVEDNVSERLGIRDLVFSKEWGEKADPDELVNYGLLEGFFYGILAPSYKNRQPWRFI
                     VDNGTVVLAVKKDIYVTEYEEKIDTAVIMLYFDAIIESTLYDIKWKFGKPEKDYKVPD
                     DYKIVAYCIV"
     gene            complement(2265..3089)
                     /locus_tag="PLD67_01030"
     CDS             complement(2265..3089)
                     /locus_tag="PLD67_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOT21140.1"
                     /translation="MEANLVVDSCVDFNEDTKDIPRVPFRIIIDEEEIVDENTDILEL
                     IRKMKKSRTQIKTACAPPEDFMNKIKVQANNFIVTISSQLSGSYNSAVMAMETIKEKF
                     PESFIHIFDSKTAAAGESLLALKVKQLIEENLNPHEIIEQTEKYISKMNTLFILESLD
                     NLAKNGRISNFKATLANIMNIFPIMGDDGEGRIVLKEKVRGRKKAFSRLVDMLAEYNI
                     DFESTVLGITHVNCLEKAENLRDAIKLKYNFKDIKIFSSAGLSTIYADDGGIVIAF"
     gene            complement(3153..3392)
                     /locus_tag="PLD67_01035"
     CDS             complement(3153..3392)
                     /locus_tag="PLD67_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOT21141.1"
                     /translation="MHYIYIVECRDKTLYTGYTTDLDKRIKTHNAKKGAKYTRGRTPV
                     ILKYYEEFNNKADAVKRESQIKKLKRAKKLILLNK"
     gene            3556..4248
                     /locus_tag="PLD67_01040"
     CDS             3556..4248
                     /locus_tag="PLD67_01040"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOT21142.1"
                     /translation="MRIGIIGAMDIEVEELINSMDDIKKETISRIDFYEGKLQEKDVV
                     VAKCGVGKVHAAICTQTMILKYAPDIIINTGVAGSLSPDLNIADIVISDGVVQHDFDI
                     SPFGKPVGLITGIDLIKIPCNEELVKQLVKAAEVLEDTNIAVGTVASGDQFICSKEKK
                     DYIVNNFNALCTEMEGASIGHVCFINDVNFCIVRAMSDKADGTANMDFDVFVKIAVAK
                     SIKLVNNFLKQL"
     gene            complement(4289..5179)
                     /locus_tag="PLD67_01045"
     CDS             complement(4289..5179)
                     /locus_tag="PLD67_01045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOT21143.1"
                     /translation="MNRYFVIYNPSSGKELAAYKVFGAAETLMNVEDAEFLFYATKKK
                     GDGEEAAKRACQYGYEMIIACGGDGTVNEVVNGMMKCCYRPKLAIMPAGTVNDFGEQL
                     NLPKAQENFASLIMQRKFKTIDVGRVNEDYFVNVVGGGAFTTISHKVTIDAKTILGKY
                     AYYYRAAVKVPDELEVCENINYIIDGKQYDYKTLLFLVLNSAGAGGFRYLCPKAELDD
                     GLLDIVIFEKTSNTELFQIFTKVFNGQHITHPKVHYYQAENIKIIPEKELLIDADGDP
                     AGYAPAEISSVHNAIDILVP"
     gene            complement(5294..6736)
                     /gene="cls"
                     /locus_tag="PLD67_01050"
     CDS             complement(5294..6736)
                     /gene="cls"
                     /locus_tag="PLD67_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOT21144.1"
                     /translation="MELFNSISLIYIVNICMAMIVIFLERKDPTATLAWVLVLLIFPG
                     FGFLLYLLLAQNFSRKQLFIMKVYAKKTFGDYIKVQKELFSTGGLVFNDKNIENYKDL
                     IKMNLFYYGFSYTQNNEIVIYTDGERKFQELFNSIENAKDHIHMEYYIIRNDDLGNKL
                     LNLLSKKAKEGVEVRLLYDSVGGRQISKDKIEKLKGSGVKVAVFFASTVPFINFKINY
                     RNHRKIVVIDGIVGFVGGFNVGNEYMGLNNKIGYWRDTHLKIRGDAVIDLQTRFFLDW
                     SHASKQELMFLPKYFPDNRIQGKVGIQIISSGPDKSDEVIKSNYVKMINSAKKSILIQ
                     TPYFIPDASVFEAIKIAAASGVDVRIMIPCKPDHPFVYWATYWYCGGLLKYGVKVYIY
                     EYGFLHAKTLVIDGTVASVGTANFDVRSFKLNFECNAIIYDTKTSQMLYDIFTDDLNY
                     SMELTRELYLERGILIRFKESVCRLLAPLL"
     gene            6867..7757
                     /locus_tag="PLD67_01055"
     CDS             6867..7757
                     /locus_tag="PLD67_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT21145.1"
                     /translation="MKKPSKYWILAGTFFTSVSSIIIRFSEAPALVISAYRMLFTCMM
                     LFLPVYIKNRNEFKSLNKSNFIMCVISGIFLAFHFASWIKSIQMTTIANSTILVSCSP
                     IFVAAINYYLFKERITYKMIVGISLSLLGTIIIGMGSSQGNESSMMLGNILAFMGAIF
                     VAGYLVIGGFVRRTVSAGVYVFIVYFVSAIVLFIMCFVTGIPVYPYPAKEYMLFFLLG
                     FFSSILGHTAYNYLMKYYSSTLISVSTLMEPIFASLMAMFIFSEIPSLYTVVGGIIII
                     GGIYYYLMTQNTIKDNEKKI"
     gene            complement(7775..>8634)
                     /locus_tag="PLD67_01060"
     CDS             complement(7775..>8634)
                     /locus_tag="PLD67_01060"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOT21146.1"
                     /translation="PLMLEKEGLAKLVCDHFNIKCTEPDLIEWTDMVERSKNPKNRVK
                     IALVGKYVELKDAYLSVAEALKHAGFDNSCDVDISWIHSDHVNENNYKQILKDCDGIL
                     VPGGFGDRGIEGKILAAKYARENRKPYLGIGLGMQTAIIEFARSVLGYADADSFEFNA
                     ESGHKIIDVTDGQENIEKNGVAMRLGSYACKLVDGTKTKEAYEGADMIHERHRHRYEF
                     NNKFRDVCQENGLIISGISPDNKLVEIVELKDHPWFVGCQFHPEFKSRPTRSHPLFKN
                     LIKASIEFK"
BASE COUNT         2588 a         1676 c         1310 g         3060 t
ORIGIN      
        1 gaaaagaaaa taattttatt tgttttctca ttaaaacctg ttgaaagtaa ataaaataac
       61 aaccccaagg gtatagaaaa caaacacata tagacatatc cgatattaaa tattttgcta
      121 tatgtaaatg cttttaaaat tgtttcataa tacagaatta ataagggaaa aaatacaagc
      181 cttaaaatat ttttgttggt attcataatg cttctttctg tcatatctat aaaattaata
      241 gctgacctga ggaaagccat gaggctttcc tcgggttact gacaaaccct atatgtcatc
      301 ctgagcgtaa gcgtcatttt actacaacat ttgctaattt ttcatgttct atgaaccttt
      361 gaatattagc tacaatcagc tcagccagtc tctttttgtt ttcctttaca attcctgatg
      421 catgaggagt gatgtacaca ttttccaaat cccacaattc atcgtcttca ggtaaaggtt
      481 ccacttcaaa aacatccaat cctgcataag agattgattt gttttttaat gcttctgtta
      541 agtctttttg atttattatg ctgccccttg ctatattgat tattgaagca ctttctttca
      601 taaatttaag agtatcctta ttaatcatat gataggtatc cttgtttaaa tcaactgtta
      661 caattatgta atcactgtta gacattaaat attccaagcc ctttttacct gatagtattt
      721 catcaaaata tgccatggca gatacgggag ttctcttgta accaattatt ttaacgccga
      781 atccctgaag tctctttgca atttctgttg caattgagcc tgttcctatt attcccacgg
      841 tttgaccgtg aaattctgtt cttttctgac gtggctccca cttgtgctct ctttgactcc
      901 ttagaaattt caaagcattt gtattatgca taagaatttt acatacaaca tcctctgcta
      961 ttggaatgga ataaatatcc tttccgttgc agaaggtaat atttcttttt ttcatatagt
     1021 ctagatccaa tgtatcgtat cctgctctga aggactgaag ccaccttata ttgggcatct
     1081 tatcaagctt ttccggtaca aaatccatcg gctctccaat tagcgcttcc gcttcggggc
     1141 attcatcaat ttcggtataa aattcaatat caggaaattt ttcagataca atgtctaaat
     1201 attttttgtg aataattatt ttcatatata ctcctttatt atataattta taatgtacgt
     1261 gtctatctct gactatctta atacacaagc agagggcata ttgcccccgg attgataaaa
     1321 agtatatttt ctaaacaatg caatacgcta caattttata atcatccgga actttataat
     1381 ctttttccgg tttcccgaac ttccatttta tgtcatacaa tgtactttct ataattgcat
     1441 caaaataaag cattataact gcagtatcta ttttttcttc atattcggtt acataaatgt
     1501 cttttttcac tgccaaaaca accgttccgt tatcaactat aaatctccaa ggctggcgat
     1561 ttttgtatga tggagccagt ataccgtaaa agaatccttc caacagacca tagttcacca
     1621 gttcatcagg gtcggctttt tctccccatt ccttagaaaa aaccaagtct cttatgccga
     1681 gtctttcgct tacattatcc tcaacaagtt ttacatttga tgaagtattg tattttgaac
     1741 ccttctcata caaaacttta ttttgtgggt cttcataacc aagtgctata agagcaacca
     1801 actttttgtc tgaatcgata cctagctttt tctttatttc ttcgccgtct ttaaaagtta
     1861 tccagcatga agcaattcct aaactccatg ctttcagcat aatatcctgt gctctgtagc
     1921 ctgcattttc tatgtaatga tccttttctt cggataagaa tatcaaataa tgaggagctt
     1981 caatcattct gttgttatat ccggcggaat cttttagctt atcgaaaact tcatctctgc
     2041 ttcttctaat tactccaact tcaatatcat caaccagtct tttgccctgc tcataatatt
     2101 gttttaattc cttaagtaat tttgaatcca cttcttcttt tttgtaaagt cttaccgatt
     2161 taatttcaga aataatctct ttatatttca tattaagttt cctccttatg ggtatattgt
     2221 aattataccc aacttttctg agaactaaca aaaatattat actgttaaaa tgctattaca
     2281 attcctccat catcggcata tatggtactc aaccctgcag aactgaatat tttaatatct
     2341 ttgaaattat attttaattt tatggcatct cttaaattct cagctttttc aaggcaatta
     2401 acatgagtta ttcctaagac tgtactttca aaatcaatat tgtattcggc aagcatatca
     2461 acaagcctgg aaaaagcttt ctttctacct cttacctttt cttttagaac tatccttcct
     2521 tctccatcat cacccatgat gggaaaaata ttcattatgt ttgcaagtgt tgctttaaag
     2581 ttgctgattc taccattctt ggccagattg tccaatgatt ccaatataaa tagagtgttc
     2641 atcttagata tatacttttc tgtttgttca attatttcat ggggatttaa gttttcctca
     2701 atcaattgtt taactttaag agcaagcaaa gattcgccgg cggcagctgt ctttgaatca
     2761 aaaatatgaa taaatgattc aggaaacttt tcttttattg tttccatcgc cattacggcg
     2821 ctgttgtaag agccgctaag ctggcttgat attgttacga taaaattatt ggcttgaact
     2881 ttaattttat tcatgaaatc ttccggagga gcacaagctg tttttatttg agtgcggctt
     2941 tttttcattt ttcttataag ttccaatata tccgtatttt catctacaat ttcttcctca
     3001 tctattataa ttctgaaagg aaccctgggg atatcttttg tatcttcatt aaaatctaca
     3061 caactatcta ctactaaatt tgcttccata ccgaacactc ctctctttat aattattatg
     3121 attataacaa aaacataaat attaaacaat ttttatttgt ttaataagat cagttttttt
     3181 gcacgtttca atttttttat ttgcgattct ctctttactg catcagcttt attgttaaat
     3241 tcctcataat attttaatat aacaggagtt cttcctctgg tgtactttgc tccttttttt
     3301 gcattatgag tttttatcct tttatccaaa tcagtcgtat atcccgtgta caacgttttg
     3361 tctctgcatt ccacaatata tatataatgc atatattctc cttttacata ataatatcca
     3421 ttttaacata tttttacaaa ggttgaaagt aaaatatttt taatatgcca tgcaatcctg
     3481 agcgtaagct gcttgtcacg gtgttgactt ttgttttttg ttatattaaa attaataata
     3541 atgaggaggc caaaaatgag aataggtata atcggagcca tggatattga ggtggaagaa
     3601 cttataaatt ccatggatga tataaaaaag gaaaccataa gccgcataga tttttatgag
     3661 ggaaaactgc aggaaaaaga tgtggtggtg gcaaaatgcg gtgtaggcaa ggtgcatgca
     3721 gctatttgca ctcaaactat gatattgaag tatgctccgg atataattat caacacaggt
     3781 gttgcaggca gtttaagccc tgacctcaac attgcggata ttgtaatatc agacggtgtg
     3841 gtacagcatg attttgatat ttcacccttt ggtaaaccgg taggtttgat taccggaatt
     3901 gatttaatta aaattccatg caatgaagaa ttggtaaaac aattagtaaa agcggctgaa
     3961 gtcttagagg atacaaatat agccgtggga actgtagctt caggcgacca gtttatctgc
     4021 agcaaggaaa agaaggatta catagtaaat aactttaatg ccctttgcac cgaaatggaa
     4081 ggggcatcaa taggtcatgt atgctttata aatgacgtaa atttctgcat agtaagagca
     4141 atgtctgaca aggctgacgg aactgccaac atggactttg acgtttttgt gaaaattgca
     4201 gtagcaaagt cgattaaact ggttaataac ttcttaaagc agctatagat ggggggtcac
     4261 accaaacaag gaatgtcccc cttcgaaatt acggaactaa tatgtctatg gcattatgta
     4321 cggaagaaat ttctgcaggt gcgtatccgg cagggtctcc atctgcatca attaataatt
     4381 ccttttcggg aattattttt atattttctg cctgataata gtgaactttt ggatgagtta
     4441 tatgctgtcc gttaaaaacc ttggtgaaaa tctgaaacaa ctcggtatta cttgtttttt
     4501 caaaaataac tatgtctaac aagccgtcat ctaattcagc cttaggacac aagtacctaa
     4561 acccgcctgc gccggcagaa ttaagtacaa ggaaaagcaa agttttatag tcatattgtt
     4621 ttccgtcaat tatataatta atattttcgc aaacctccaa ttcgtccgga accttaacag
     4681 ctgcccgata gtaataggca tactttccaa gtattgtctt tgcatcgatt gttaccttgt
     4741 gtgaaatagt agtaaaagct cctcctccta ctacatttac aaaataatct tcatttactc
     4801 tccccacatc gattgtttta aatttccttt gcataataag agaggcaaaa ttttcttgag
     4861 cttttggaag gttaagctgc tcgccaaagt cattgactgt tcctgccggc attattgcca
     4921 atttcggtct gtagcagcat ttcatcatcc cattgacaac ttcatttact gttccgtcac
     4981 cgccgcatgc tataatcatc tcatagccgt attgacacgc tcttttggca gcttcttctc
     5041 catctccttt ttttttggtt gcataaaata aaaactctgc atcctcaacg ttcattagtg
     5101 tttcggctgc accaaatact ttatatgctg caagttcttt tcccgaagat ggattgtata
     5161 ttacaaaata tctattcata cttatcacct gtatctttca tgtttcaccg cctaatgggg
     5221 caggatatct ttgtcttggt tatataatta tagcatttca gttaaaaatt tcaacaatta
     5281 atcttctaca aacttataat aaaggggcca atagtctgca tacagattcc ttaaatctta
     5341 ttaaaatgcc cctctccaag tacaactctc ttgtcagctc cattgaataa tttaaatcat
     5401 ccgtgaatat atcatacagc atttgggatg tctttgtatc atatattatt gcattgcact
     5461 cgaaattaag tttaaaactt cttacgtcaa aatttgcagt tcccacagat gcaacagtgc
     5521 cgtcaatcac cagggtcttg gcatgtaaaa atccgtattc ataaatataa accttgactc
     5581 catactttaa caatcctccg caataccagt aagtagccca gtaaacaaaa ggatggtcag
     5641 gtttgcaagg tatcataatc cgaacatcca cccccgatgc agcagcgatt ttgatagctt
     5701 caaatacaga ggcatcagga ataaaataag gagtttgaat taaaatactt tttttagccg
     5761 aatttatcat tttcacataa ttacttttta taacttcatc cgatttgtca ggtccgctgc
     5821 ttattatttg aattcccacc ttgccttgaa tcctgttgtc gggaaaatat ttcggcaaga
     5881 acattaactc ctgtttggaa gcatggctcc agtcaaggaa aaaccttgtc tgcaggtcta
     5941 ttaccgcatc tcctctgatt ttaagatgag tgtctctcca ataaccgatt ttattattta
     6001 agcccatata ctcattccct acattaaatc ctcccacaaa acctactata ccgtcaataa
     6061 caactatttt cctgtgattt ctgtaattga ttttaaagtt aatgaaagga acggtacttg
     6121 caaaaaatac agctacctta accccggaac ctttcaactt ttcaatttta tctttgctta
     6181 tctgcctccc gcccactgaa tcatacaaaa gccttacctc cactccttcc ttcgcctttt
     6241 ttgaaagaag attaagaagt ttgtttccaa ggtcatcgtt tcggattata tagtattcca
     6301 tatgtatgtg gtcctttgca ttttctatgg agttaaaaag ctcttgaaac ttcctttcac
     6361 cgtctgtata aattacaatc tcattgtttt gtgtatagga aaagccataa taaaataaat
     6421 tcatttttat taaatcttta tagttttcta tattcttatc attaaaaaca agtccccctg
     6481 tactgaacaa ttctttttga accttaatat aatctccaaa tgtttttttg gcataaactt
     6541 tcataataaa gagctgcttc ctgctgaagt tttgtgccag caacaaatac aacaaaaatc
     6601 cgaagccggg gaatatcaat agaaccaata cccatgcaag tgttgcggta gggtcttttc
     6661 tttccaaaaa tattactatc attgccatac aaatatttac tatataaatc aatgaaatag
     6721 agttaaataa ttccacaata atcctcccga actacctttt tttattatta tatgaacatc
     6781 tcaattagta taaacattat cccaatgatt gaaaatgtaa aaagtttgtg gtagtatact
     6841 tattatcaac acaaaaggaa gaaattatga aaaaaccatc aaagtattgg attcttgcag
     6901 gtacattttt tacttctgtt tcttcaataa ttataagatt ctctgaagca cctgctctgg
     6961 ttatttcagc ttacagaatg ctctttactt gtatgatgct ctttttgcct gtctacataa
     7021 aaaacagaaa tgaatttaaa agtctgaata aaagcaactt catcatgtgt gttataagcg
     7081 gaatatttct tgcatttcac tttgcttcct ggattaagtc gattcaaatg actacaattg
     7141 caaattcaac cattcttgta tcttgcagcc cgatatttgt agcagcaatc aactattatc
     7201 tgtttaaaga aagaataacc tataagatga tagttggtat ttctttgtcc ctgttaggta
     7261 caataattat aggaatgggt tcatctcaag gaaatgaaag cagcatgatg cttggaaata
     7321 tcctggcatt catgggtgca atatttgttg ctggttattt ggtaataggc ggatttgtaa
     7381 gaaggaccgt aagtgcaggg gtatatgttt ttattgttta ttttgtatcg gcaattgtat
     7441 tgtttattat gtgctttgta acgggaatac ccgtatatcc ataccccgcc aaagagtata
     7501 tgttattttt cctcttaggt tttttcagct caatacttgg ccatacagct tacaattatt
     7561 taatgaaata ttattcatct acgttaattt ccgtatcaac attaatggag ccgattttcg
     7621 ccagccttat ggcaatgttt atttttagtg aaataccatc cttatatacg gtggtcggcg
     7681 ggattataat aattggaggc atatattatt atctgatgac acaaaacaca ataaaagata
     7741 acgaaaaaaa aatataagac agtgagtcac tgtcttattt aaattctatc gaagctttta
     7801 ttaaattctt gaacaaagga tggcttcttg taggtcttga tttgaattcc ggatggaact
     7861 gacaacctac aaaccaagga tggtccttta gctctactat ttcaaccaat ttgttgtccg
     7921 gtgaaattcc tgaaattata agtccgttct cctgacacac gtccctgaat ttattgttaa
     7981 actcatatct gtggcgatgc ctttcatgta tcatgtcagc accttcatat gcttcttttg
     8041 tcttagtgcc gtcaacaagc ttgcacgcgt agcttccaag cctcatcgct acgccatttt
     8101 tttcgatatt ttcctgccca tccgttacat caataatttt atgaccggat tcagcattaa
     8161 attcaaagct gtcggcatca gcatatccta agactgacct ggcaaattca ataattgctg
     8221 tttgcatgcc aagtcctatt cctaaatagg gctttctatt ttctcttgca tattttgccg
     8281 caagaatttt accttctatt cccctgtcgc caaaaccgcc gggaactaat attccgtcgc
     8341 aatccttaag tatttgttta tagttgtttt cattgacatg atctgagtgt atccaagaaa
     8401 tatcaacatc gcaagaatta tcaaatcctg catgtttgag tgcttccgca actgacaaat
     8461 atgcatcctt aagctctaca tacttgccca caagtgctat tttaacacga ttttttggat
     8521 ttttagagcg ctcaaccata tcagtccact ctattaaatc cggctctgta catttaatgt
     8581 tgaaatggtc gcagactaat tttgccaggc cttccttttc caacattaaa ggaa
//
LOCUS       DAOSOW010000018         8633 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_8137_length_8633_cov_3.991373, whole genome shotgun sequence.
ACCESSION   DAOSOW010000018 DAOSOW010000000
VERSION     DAOSOW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8633
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8137_length_8633_cov_3.991373"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(237..989)
                     /locus_tag="PLD67_01065"
     CDS             complement(237..989)
                     /locus_tag="PLD67_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOT21147.1"
                     /translation="MNEFSRTQLLLGKQAMEKLSSSTVAVFGIGGVGTFVVEALARCG
                     VGNFALFDDDKVCITNINRQLIATRKTIGRKKVEVMAERIKEINPKASTATYQVFYMP
                     DNADEYDLTSYDYIVDAVDTVTAKIELIVRAKKNNIPIISSMGAGNKLNPTAFEVADI
                     YKTSVDPLAKVLRHELKRRGIENLKVVYSKEKPLTPSCDEDSCKDSCVCPPGTTRKCT
                     QRRAIPGSVSFVPSAAGLIIASEVVKDIIAGI"
     gene            complement(1168..1635)
                     /locus_tag="PLD67_01070"
     CDS             complement(1168..1635)
                     /locus_tag="PLD67_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HOT21148.1"
                     /translation="MSVEKVREYLKQFNKENDIKEMDESTATVELAALALDTEEARIA
                     KSLSFYNGDGAMVIVVAGDAKIDNRKFKDYFGFKARMLSPEDTKKFTGHEVGGVCPFA
                     LPDNVKVYLDVSMKRFETMFPACGSGNSAIELTLDELEETSKSNTWIDVCKNW"
     gene            complement(1692..2459)
                     /gene="pflA"
                     /locus_tag="PLD67_01075"
     CDS             complement(1692..2459)
                     /gene="pflA"
                     /locus_tag="PLD67_01075"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HOT21149.1"
                     /translation="MTAGFLHSIETLGTLDGPGLRMIVFLQGCKLRCLYCHNPDTWIE
                     NITPGDNKNLTDKYYLKERFTADYIAEKAKRYKPYFKNNGGITFSGGEPLRQPKFLLD
                     CLMLCKAEGIHTVIDTSGVGFGDYDEILKYTDLAILDIKHSNPEKYKKITGCDIEYYL
                     DFKEAVIRNKIQLWIKHVVTPGINDTEGDMKDFENEVKTFPDKLIQKIELLPYHTLGV
                     YKYKELNIKYKLEGVPPLSKDRLQELKDCITLEKLIR"
     gene            complement(2462..4687)
                     /gene="pflB"
                     /locus_tag="PLD67_01080"
     CDS             complement(2462..4687)
                     /gene="pflB"
                     /locus_tag="PLD67_01080"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HOT21150.1"
                     /translation="MKEWNNFIQGRWTKEIDLRDFIQKNYSPYEGNEEFLSGATEKTK
                     NLLKKVSRLMTEELKKDVLDVDTDNISGINNFEPGYLDKDNEIIVGFQTDAPLKRMIN
                     PYGGIRMVEQSLEAYGYSMSDELCNVFHKYRKTHNEGVFDVYTPEILLARSSGLLTGL
                     PDAYGRGRIIGDYRRIALYGIDFLVEKKKEEYNNIKFPMNEDSIRAREEIKEQIKALF
                     DMKSMASKYGIDISKPSSNALEAVQAVYFGYLAGVKENNGAAMSLGRTSTFIDIYIER
                     DIKEGILSEKEAQEIIDQFVLKLRMVRHLRTPEYNELFAGDPNWVTEALGGMGLDGRT
                     LVTKTSFRFLHTLTNLSAAPEPNMTVLWSERLPEGFKKYCTKMSIDTSAVQYENDDIM
                     RPIYGDDYGIACCVSAMAIGKEMQFFGARCNLAKALLLGINGGTDEISKETVITNINK
                     INSKILNYDEVLNNYKKVLEYVAELYVNTMNIIHYMHDKYAYESGLMALHNYDVRRLM
                     AFGVAGLSIAADSLSAIKYAKVKPVYGEDGITEDFEIEGEYPMYGNDDDRVDGIAAEL
                     LEFFSNELKKHKTYRNAKHTLSVLTITSNVVYGKKTGATPDGRKKGEPFAPGANPMNG
                     RDKSGLLASLNSVAKLKYKDICEDGISNTFTVVPDTLGKKYRHRIDNLSNILDGYFSQ
                     GAHHINVNVINRDTLYEAMEAPHKYPNLTIRVSGYAVNFNRLTREQQMDVIGRTFHDR
                     M"
     regulatory      complement(4743..4825)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(4868..5749)
                     /locus_tag="PLD67_01085"
     CDS             complement(4868..5749)
                     /locus_tag="PLD67_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004041390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT21151.1"
                     /translation="MDDIIIEDNLEDEVESWEPNFWERLFSNKMAVFGATVILILFIT
                     AIFAPLIAPYPYDAIDLPQMLKAPNSENILGTDEFGRDIFSRIVYGSRISLQVGFIAV
                     GISLVIGTILGALAGYYGGITDYIISGITDIAYSFPISLLAIAFVAALGRSLTNLIFA
                     VALVSWSGYARLVRGQFISLKNREFIEATKILGMSDARIIFKHMLPNSMAPVIVLTTL
                     EIPKAIIVESSLSFLGLGVPPPAPSWGSIMSSGRAYIMEAPWVSIFPGLVMALAVLGF
                     NLFGDALRDTLDPRLKD"
     gene            complement(5791..6708)
                     /locus_tag="PLD67_01090"
     CDS             complement(5791..6708)
                     /locus_tag="PLD67_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015326155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT21152.1"
                     /translation="MLKYIFKKLLMMIPILLGLTIILFLILHLAPGDPVHLVVGPNVT
                     PEVYENVRRSMGLDRPLIIQYFDYLSNLVKGDLGSSILQHRPVADIVLERFPITIGLG
                     VRALLLSFLIAVPVGIIAAVNRNKSADFIAMTFALVGISMPTFWFGIILLYFFAYKLG
                     LFPISGYGTWKHLVLPVITMGLTDAAVIARMMRSSMLEVIGQDYIRTARAKGLREKVV
                     TNKHALKNALIPVITLLGMRMGWIIGGSVTLEIIFSIPGLGRTMVDSIFSRDYPVVQG
                     SVLILATSVMISNLIADILYALVDPRIRY"
     gene            complement(6769..8370)
                     /locus_tag="PLD67_01095"
     CDS             complement(6769..8370)
                     /locus_tag="PLD67_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT21153.1"
                     /translation="MKKNISKMFALVLILSCTALVFSGCSKNDVPVGETPEGNNGEQP
                     AKETTLVIAYERDAETLNHIKTGWYSDSLIYMYDRLVSRDYDFNYRPSLAISWETSED
                     GMVWTFHLRDDVKFHNGKPLTAHDVKFTFDTILDPETASPSQSDFAAIKGLEVKDEHT
                     VDIMLHQPFPNLLFVLSNTAAGIVNKEAYEEYGDEYGINYVVGTGPYMFKEWKQGDKI
                     IIVKNPDYNWGPEWMTNRGPALIDRIEFRTIPEENTRLMELEAGNVHILRDVPASFLQ
                     RVEEIDDVEVMRRDATKLGYLAYACDKEPFTDIRVRRAINHAINKEDIIKYVFRGYGE
                     PAYGYLPPALKSEYYADSKKDGYEYDVEKAKQLLKEAGYGDGLKLTLSAENSTEYSRL
                     AEVLQDQLKQVGIETEIQLYDSSSYTSMLREGQQELFIRLYSWPNADILDWFLLSSQF
                     PYPNHSRWQDEKTDELINKAATSPTWDERSKGYFEVQKHLIEQAVWAPIYIPENTIAV
                     RKEVKDFKFHPWIPQYNDGTYLEVE"
     gene            complement(8375..>8633)
                     /locus_tag="PLD67_01100"
     CDS             complement(8375..>8633)
                     /locus_tag="PLD67_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT21154.1"
                     /translation="LFKRPAHPYTKALLSSIPVCDPDFRKNIILLEGDLPSPANPPKG
                     CRFHTRCIYAREDCSRKEPVSVNLGNGHMVECHLYSNEEED"
BASE COUNT         2401 a         1917 c         1361 g         2954 t
ORIGIN      
        1 tgagcgacta ccaacttcca tttttagacc tctctttcat tattgtatac aatattttgt
       61 atattttatt tttgttttta ttataaataa taccagtaga cattgtcaag taaattatac
      121 ttaaatcaag tagttctttt tatcgttaag gatgattatt aatttctcat acaaaaaaac
      181 tctacaaatg cataaaacat ttgtagagga tatatatgaa aattattcgt taacttttat
      241 atgccggcta taatatcttt aacaacctcg cttgcaataa tcagccctgc tgcagaaggt
      301 acaaatgata cacttcccgg tattgcacgt ctttgggtgc attttcttgt ggttccgggc
      361 gggcatacgc agctgtcctt gcagctgtct tcatcgcagc ttggagtcag aggtttttct
      421 tttgaatata ctacctttaa attttcaata ccccgccttt ttaattcatg acgcaatacc
      481 ttcgctaagg ggtctaccga tgttttgtaa atatcggcaa cttcaaaggc agtgggattt
      541 aatttgtttc ctgctcccat gctgctgata atgggaatat tgtttttttt tgctcttact
      601 atcaattcta ttttagcggt aacagtatca actgcatcta caatataatc ataagaagtt
      661 aaatcatatt catccgcatt gtcaggcatg taaaaaacct ggtaagttgc tgtactggct
      721 tttggattta tttccttaat cctttctgcc atcacttcaa ccttcttcct gccaatggtt
      781 tttcttgttg caattaactg cctgtttatg tttgttatgc atactttgtc gtcatcaaac
      841 agtgcaaaat ttcctacgcc gcatcttgcc aaagcttcaa caacaaatgt gcctactcct
      901 ccgatgccga atactgcaac tgtggaagag cttaattttt ccattgcttg tttacctaat
      961 agtagctgtg ttcttgaaaa ttcgttcata ttatcctttc tttttatgtc attgttatga
     1021 gtaacccata gagcgtcatt ctgaacccta ggtgaagaat ctcatctttt aaaatctgag
     1081 atccttcgct tacgctcagg atgacacggg ctgcgccctc aaaattacaa ttattaataa
     1141 aagttacaaa tctttgattg ctctttatca ccaattttta catacatcaa tccaggtgtt
     1201 gctttttgat gtttcctcca attcatcaag ggtgagctct attgcagagt taccgcttcc
     1261 acaggcagga aacatagttt caaatctttt cattgataca tccaagtaaa cttttacatt
     1321 atcaggcagt gcaaaaggac aaacccctcc gacttcatgt ccggtgaatt tttttgtatc
     1381 ttcaggtgaa agcattcttg ctttaaaccc gaaataatct ttgaattttc tattatctat
     1441 ctttgcatct cctgctacta ctattaccat tgctccatca ccgttataaa atgataatga
     1501 ttttgcaatt ctcgcttcct ccgtatctaa ggccagagca gccaactcta cggtcgctgt
     1561 tgattcgtcc atttctttta tgtcattttc cttgttaaat tgttttaaat actctcttac
     1621 cttttctact gacatttttc cctcctaata cttctttatg ctatctatcg ctatgtcaaa
     1681 tacttctttt attatcttat cagtttttcc aaagtaatac aatccttaag ttcttgaagc
     1741 ctgtcttttg acagaggagg gaccccttcc aatttatatt ttatatttag ttccttgtat
     1801 ttatacactc ccaatgtgtg ataaggcagc agctcgattt tttgaatcaa tttatcagga
     1861 aaagttttca cttcattctc aaagtctttc atatctcctt ctgtatcatt aattcccggc
     1921 gttacaacat gtttaatcca aagctgtatt ttatttctga ttaccgcttc cttaaagtcc
     1981 agataatatt caatatcgca gccggttatc tttttatatt tttcaggatt tgaatgcttt
     2041 atgtcgagta ttgccaaatc ggtatatttt aatatttcat cataatctcc aaaacctact
     2101 cccgatgtat ctataaccgt atggattcct tcagctttgc acagcataag gcaatccagc
     2161 aaaaattttg gctgcctcaa tggctcacct cctgaaaaag ttatgccccc gttattctta
     2221 aaataaggtt tatacctttt cgctttttca gcaatataat ctgcagtaaa cctttctttg
     2281 agataatatt tatctgtcaa gtttttattg tcgcctggag tgatgttttc tatccatgtg
     2341 tccgggttat gacaataaag acatcttaat ttgcacccct gtaaaaacac tatcattcta
     2401 agccccggtc cgtccaaagt acccaatgtt tctattgaat gaagaaatcc ggcagtcatt
     2461 tttacatcct gtcgtggaat gttcttccta ttacatccat ttgctgctca cgagtcagtc
     2521 tgttaaagtt tactgcgtaa ccggaaactc ttatagttaa gttcggatat ttatggggag
     2581 cttccatggc ttcataaaga gtatccctgt taattacatt aacatttata tgatgagccc
     2641 cctgagaaaa atatccgtcc agtatatttg acaaattatc tattctgtgt ctgtattttt
     2701 ttcccaatgt atcgggaact accgtaaaag tatttgaaat tccatcttcg caaatatctt
     2761 tatactttaa ttttgcaacc gaattcaagg atgcaagcaa tccgctttta tcccttccgt
     2821 tcattggatt tgctcccggt gcaaagggct ctcctttttt tctcccgtca ggtgttgctc
     2881 ctgttttctt gccgtaaaca acattcgatg taatagttaa tactgaaagt gtatgctttg
     2941 cattcctgta ggttttatgt ttttttaatt cattagagaa aaattccaat aattccgcag
     3001 caataccgtc aacacggtca tcgtcattac cgtacattgg atattctccc tctatttcaa
     3061 aatcctcggt tataccgtct tcgccgtaaa caggttttac ctttgcatat tttatggcac
     3121 tcagagagtc tgcagcaatt gaaagccctg caactccaaa ggccatcaac cttctcacat
     3181 catagttgtg aagagccata agtcctgatt cgtaagcata tttatcatgc atgtaatgaa
     3241 ttatgttcat agtattaaca taaagctccg ctacatattc cagtactttt ttgtagttgt
     3301 tgagcacttc gtcataattg agtatttttg aatttatttt atttatattt gttattactg
     3361 tttctttact gatttcatct gtaccgccgt taatccctaa gagcagtgct tttgccaaat
     3421 tgcatcttgc cccgaaaaat tgcatttctt tgcctattgc cattgcggat acgcagcatg
     3481 caataccgta atcatcaccg taaataggtc tcattatgtc atcgttttcg tattgaacag
     3541 cagatgtatc aatggacatc ttagtgcaat attttttgaa accttcaggc agcctttccg
     3601 accacaaaac tgtcatattc ggttccggtg cagccgacaa atttgttaat gtgtgcaaaa
     3661 acctgaaaga tgtcttagtt accaatgttc tcccatcaag tcccattcct cccaatgcct
     3721 cggtaaccca gttcgggtcc cctgcaaaca actcattata ttcgggagtt cttaagtgac
     3781 gaaccattct aagttttaaa acaaactggt caataatttc ttgagcttct ttttcgctga
     3841 gtataccttc ttttatatcc ctttctatat atatgtctat aaatgtgctt gtcctgccta
     3901 atgacattgc agctccgtta ttttctttca cccctgccaa atatccgaaa tagactgcct
     3961 gtacagcttc caatgcattt gaagaaggtt ttgaaatatc aataccatat ttagatgcca
     4021 ttgatttcat gtcaaataat gctttaatct gttctttaat ttcttccctt gctctgatgc
     4081 tatcttcatt catagggaat ttaatattat tatattcttc cttctttttc tcaaccagaa
     4141 aatctatgcc gtacaaagca attcttctat agtcgccgat tattcttcct ctcccgtatg
     4201 catcgggaag acctgtcaaa agtccggagc ttctggccaa tagtatttca ggtgtgtata
     4261 catcaaatac tccttcgttg tgagtttttc tgtatttatg aaaaacatta cataactcat
     4321 cgctcataga gtatccgtag gcttccaaag actgctccac cattctgatt ccgccgtatg
     4381 gattaatcat cctcttcagg ggtgcatctg tttgaaatcc cacgattatt tcattgtcct
     4441 tgtctagata tcctggctca aaattattaa ttcccgaaat attatcggta tccacatcca
     4501 aaacatcttt tttaagttct tctgtcataa gccttgatac tttttttaat aagtttttgg
     4561 ttttttctgt tgcgccgctt aagaattctt catttccttc gtaagggctg taattttttt
     4621 gaataaaatc tcttaggtca atttcttttg tccatcttcc ttgaataaaa ttgttccact
     4681 ctttcatagt tcctcctatg gtttttccgg gaggctatgt tttgaaattt aaaaagccgc
     4741 ccgggattag cccaggcggc cgacttttaa ttttattcac acttcctcgt ggtactccac
     4801 ttttccgcca gtcatgtgat actcttatgt tacaataaat aacatttttt gtcaatactt
     4861 tatgctctta atctttcagt ctcggatcaa gcgtatctct caatgcatct ccgaaaagat
     4921 tgaatcccaa tactgccaat gccatcacta atcccggaaa aatagagacc caaggtgctt
     4981 ccataatata tgcacgcccc gaactcataa tcgagcccca gctgggggcc ggaggcggca
     5041 cacctagacc gaggaaactc aaagaagatt ccactataat cgctttaggt atttcgagtg
     5101 tagtaagaac aataacaggt gccatcgaat taggaagcat atgcttgaaa attatacgcg
     5161 catcactcat tcccagtatc tttgttgctt cgataaattc acggttcttt aatgatataa
     5221 actgtcctcg cacaagtctt gcataaccag accaactaac cagtgctact gcaaaaatca
     5281 aatttgttaa gcttcgacca agtgccgcaa caaaggcaat ggcaaggagg cttataggaa
     5341 atgaataagc aatatcggta attcctgaaa taatatagtc cgtaattccc ccgtaatagc
     5401 ctgccaatgc tcccaaaatt gtgccgatta ccaatgaaat acctaccgca ataaatccta
     5461 cctgtaggga aattctggag ccgtaaacaa tccggctgaa tatatccctc ccgaattcat
     5521 ccgttcctaa aatattttca gagttaggtg ctttcaacat ctggggcagg tcaatggcat
     5581 catagggata tggtgcaata agcggggcaa aaatagcggt aataaacaaa attaatatta
     5641 ctgtagcacc aaaaacagcc attttattag aaaaaagcct ttcccaaaaa ttgggctccc
     5701 aactttccac ttcatcttcc aaattgtctt ctataataat gtcatccata ttatactcca
     5761 tcttttttaa tacttttata taaactagat ttaatacctt atcctggggt caacaagggc
     5821 ataaagaata tctgctatta aattgcttat cattaccgat gttgcaagta ttaatactga
     5881 gccttgaaca acagggtagt ctcttgaaaa aattgaatca accatcgtcc ttcccaaacc
     5941 gggtatgctg aaaataattt ccaaagtgac cgagcctcca ataatccagc ccattcgcat
     6001 gcctaagagg gttatgacag gtatcaatgc atttttaagc gcatgcttgt ttgttacaac
     6061 tttttctctc aacccttttg ctcttgccgt ccttatatag tcctgaccaa taacttccaa
     6121 catgctggac cgcatcatcc tggctattac cgctgcatcc gtgagcccca tggtaataac
     6181 gggcagcact aaatgtttcc aggtaccata acctgatatc ggaaacaacc ctaatttata
     6241 tgcaaagaaa taaagcagta ttattccaaa ccaaaatgtg ggcattgata tgccgaccaa
     6301 agcaaaagtc atagctataa aatctgctga cttgtttctg ttaactgctg caataattcc
     6361 aacaggaacg gcaattaaaa aggaaagtag aagtgccctc actcctaaac cgattgttat
     6421 agggaaacgt tccaaaacaa tatctgccac aggtctatgc tgcaaaattg agctgcctaa
     6481 atctcccttt actaaattac tcaagtaatc aaaatattgt attatgagcg gcctgtctaa
     6541 acccatgctc cttctgacat tctcataaac ttcaggagtc acgttaggtc ctacgactaa
     6601 atgcaccggg tctcccggag ccaaatgtaa aattaaaaat aaaataatag tcaatcctaa
     6661 caaaatcggt atcatcataa ggagcttttt aaatatatat ttaagcatta attcacctca
     6721 atcgtaatta ttgcaaagct tccggatata ccggaagctt tgcctgctct attcaacttc
     6781 caaatatgta ccgtcattat attgaggtat ccaaggatga aacttgaaat ctttaacttc
     6841 ttttcttact gcaatggtat tttcaggtat ataaatcggg gcccatactg cctgttcaat
     6901 caaatgcttt tgaacttcaa aatatccttt agatctttca tcccatgtag gacttgtagc
     6961 tgctttatta attaactcat cggtcttttc atcctgccat cttgaatggt tcgggtatgg
     7021 gaactggctc gataggagga accaatccaa tatatcggca ttcggccagc tgtaaagcct
     7081 tatgaaaagc tcctgttgac cttctcttaa cattgacgta tagcttgaac tgtcatatag
     7141 ttgaatttct gtttcaattc caacctgttt tagctggtct tgaagcactt ccgctaaacg
     7201 gctgtactcc gtagaatttt ctgccgataa tgtaagcttg agcccgtctc catagccggc
     7261 ttcctttaac aattgctttg ctttctccac atcatactca tatccatcct ttttactgtc
     7321 cgcataatat tcgcttttca gggcaggagg caaataaccg taagcaggct ctccatatcc
     7381 tctaaaaacg tatttaataa tatcttcttt attaattgca tggtttattg cccgccttac
     7441 tctgatgtcg gtaaaaggct ccttatcgca ggcataagcc aaatatccaa gttttgtagc
     7501 atctcttctc attacttcta catcgtcaat ttcttcaact ctttgcaaaa atgatgcagg
     7561 gacatccctc aatatatgta catttcctgc ttctaactcc atcagccgcg tattttcttc
     7621 cggtattgtt ctgaattcta ttctgtcgat taaagccggt cccctgttgg tcatccattc
     7681 aggtccccaa ttgtaatcgg gattctttac tattataatt ttatctcctt gtttccattc
     7741 tttgaacata tagggtcctg tgcctactac ataattaatt ccatactcat caccatactc
     7801 ttcataagct tctttgttta ctattcctgc tgccgtattt gacaaaacaa acaagagatt
     7861 tggaaaaggt tgatgcagca ttatatctac cgtgtgttca tcctttactt ccaatccctt
     7921 tatcgctgcg aaatcgcttt gagaaggaga tgcagtttca gggtctaaaa tagtgtcaaa
     7981 agtaaatttt acatcatgtg cagtcaaagg ctttccgtta tgaaatttta catcgtctct
     8041 caaatgaaat gtccacacca ttccatcttc cgaagtttcc caacttatag ccaaactcgg
     8101 tctgtaatta aaatcgtagt ccctggaaac cagcctgtca tacatataaa tcaaagaatc
     8161 tgaataccag cctgttttta tgtgattcag ggtttccgca tctctttcgt aggctataac
     8221 cagggttgtc tcttttgccg gttgctcacc gttgttacct tccggcgttt cgccgacggg
     8281 cacatcattt ttcgaacatc ctgaaaaaac taatgctgta cacgataaaa tcagtaccaa
     8341 tgcaaacatt ttcgaaatat tttttttcat tgaactaatc ctcctcttca tttgaatata
     8401 aatgacattc caccatatgt ccgtttccca ggttgacaga tacaggttcc ttccttgaac
     8461 aatcttccct agcataaata catctggtat gaaatcgaca tccctttgga ggattagccg
     8521 gactaggcaa atcaccctcc aaaagaatta tattcttacg aaagtcaggg tcacatacgg
     8581 ggattgacga taacagtgct tttgtatagg gatgagccgg ccttttaaat aac
//
LOCUS       DAOSOW010000019         8553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_8211_length_8553_cov_3.525888, whole genome shotgun sequence.
ACCESSION   DAOSOW010000019 DAOSOW010000000
VERSION     DAOSOW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8553
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8211_length_8553_cov_3.525888"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..484
                     /locus_tag="PLD67_01105"
     CDS             191..484
                     /locus_tag="PLD67_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21155.1"
                     /translation="MDKESLIFEGIIRKLNIDEADDKIIENKTNTTYKALISCITTLP
                     PKQFVLLSTLIGILLIDDIDPKDQIILGKFINNIGQTILTAAAQEQINIKKEN"
     gene            complement(612..1676)
                     /locus_tag="PLD67_01110"
     CDS             complement(612..1676)
                     /locus_tag="PLD67_01110"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012684330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HOT21156.1"
                     /translation="MNKIKLAILFGGKSDEYSVSLHSAAAIIDNVPKELYEVTLVGIT
                     TEGKWLHYEGSTDKINNDTWHQGNLSPVLLSMGDFKGLIKLNERDKVYEKIEIDCIFP
                     VLHGRNGEDGTIQGMCQMAGIPFVGCDMTSSAVSMDKEYTHMICEMSGIKTAPFMSVV
                     NHKTLNIKNLYKEALEKLSLPLFIKPANNGSSIGISKVRNYDEFEKGIMEAFEYDKKI
                     VIESNIEGFEIGCAVVGNEELLIGEVDEIETKNDFFDYVEKYSQHNSKIHCPARISDD
                     LKAQAKEIAANTYKALGCRGLSRVDMFLTPDNEIYLNEINTIPGLTDLSRYPSMLKKI
                     GIEYKDLIVKLVELAMERDE"
     gene            complement(1669..3060)
                     /gene="murF"
                     /locus_tag="PLD67_01115"
     CDS             complement(1669..3060)
                     /gene="murF"
                     /locus_tag="PLD67_01115"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOT21157.1"
                     /translation="MISINIKEAADILNAQCTGTYDSDKHIKGVSIDSRNVTENSLFI
                     PIKGMTVNGHAFVKDAIKKGAAATLWNKDEPNPPHDIAVILVEDTVKGLQELSRYYRN
                     KLNTKIVGVTGSNGKTSTKDITAAILSQKFKTQKTIGNYNTEIGVPFTLLSLDEDCQA
                     AVIEMGMERKGEIDFLADLVQPDIAILTSVGLVHIDNFPSIEEIAKAKLEIVNGIKDK
                     GLFIYYGDDTLIEETVKNTQIKKSIRKQTFGLNKNNTIWLKEFKQDLEGITIKVSDER
                     FDELHIDILGKHQALNAMAAILAALELGMNPEEIKSGLLRIEKTGLRNEVININKCNI
                     LNDCYKSNPVSIKAALDIFELFNSKKKIAVLGDMLGYREMSHDVHYKVGRDLSRHHID
                     ELVTIGQEAKFIAKAAMENTNIKSIVEFDTKEEAALYLKKYLIEDCTILVKGSRFLKL
                     EYIANKLKEWENE"
     gene            complement(3216..3449)
                     /gene="tlp"
                     /locus_tag="PLD67_01120"
     CDS             complement(3216..3449)
                     /gene="tlp"
                     /locus_tag="PLD67_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small acid-soluble spore protein Tlp"
                     /protein_id="HOT21158.1"
                     /translation="MDKRPKPDDRRDNVERIQFNIDNTIRNYRETKDKIKITEDEKQR
                     QELEEKNERRDQALKSMRKEIRQEATDRKNNYK"
     gene            complement(3625..7191)
                     /gene="nifJ"
                     /locus_tag="PLD67_01125"
     CDS             complement(3625..7191)
                     /gene="nifJ"
                     /locus_tag="PLD67_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOT21159.1"
                     /translation="MSKVMKTMDGNTAAAYISYAFTEVAAIFPITPSSPMPELIDEWA
                     AHGQKNIFGQTVRVTELESEAGAAGAVHGSLQAGALTSTYTASQGLLLMIPNMYKIAG
                     ELLPGVFHVTARAVAGHALSIFGDHSDVMAARQTGFALLASGSVQEVMDISAVAHLTA
                     IKSRVPFLNFFDGFRTSHEIQKIEVIDYEDLAKLVDMDAVDEFRKRALNPEHPVTRGT
                     AQNPDIFFQAKEASNKYYEVVPDIVNDYMKEISKLTGREYKPFVYYGAEDADRVIVAI
                     GSVTETIKEVIDYLSAKGEKVGLVAVHLYRPFSEKYFFNVLPKSVKKIAVLDRTKEPG
                     SVGEPLYLDVKSLFYNKEDAPVIVGGRYGLGSKDTTPAQIFAVYNNLIADEPKDGFTI
                     GIVDDVTFKSLEIPEVVNVSAPGTVRCKFWGLGSDGTVGANKDAIKIIGDHTDMYAQG
                     YFSYDSKKSGGVTVSHLRFGKSPIRSTYLIDEADFVSLSQQSYVDKYDVLEGLKKGGT
                     FLLNTIWSKDELDAYLPAKYKKYMAENDIKFYTINATQIAQDLGLGNRTNMIIQSAFF
                     KLADVIPIDDAVNYLYDAIESSYGKKGTTVVEMNRAAVDKGITELVKIDIPEEWKNAV
                     DDNKANSGLAVPFTEDEKPEFIKNVAEVMTRQQGDKLPVSTFAGREDGTFPHGTAAYE
                     KRGIATSVPRWIPENCIQCNQCSFICPHSVIRPTLLDENEKANAPKDFVTIKAVGKGL
                     EGLDYRIQISTLDCTGCGNCADICPGKKGNKALVMTPIAEEVEREVPNWKYAIDKVTI
                     KDDLMDKVSVKGSQFCKPYFEFSGACAGCGETAYVKTITQLFGDRMMIANATGCSSIW
                     GASAPSTPYTKDACGKGPSWANSLFEDNAEYGYGMATAVRQIRDSIQMSMEELLELDA
                     KPELKEAIQEWIANRELGEESKAASAKVLEILEGLEPCDKCAQAIKNILDKKDYLAKK
                     SQWIIGGDGWAYDIGYGGLDHVLASGEDVNVLVLDTEVYSNTGGQSSKATPTASVAKF
                     AAAGKRVKKKDLGLIATTYGYVYVAQVAIGANMNQFLKAIKEAESYQGPSLIIAYAPC
                     INHGIKAGMGKTVDREKKAVEAGYWHLYRFNPLLKEEGKNPFILDSKEPTASFREFLE
                     GEVRYASLKTSFPELAEELYAKAEKDAKDRYESYVRMAEMKY"
     gene            complement(7310..8221)
                     /gene="hprK"
                     /locus_tag="PLD67_01130"
     CDS             complement(7310..8221)
                     /gene="hprK"
                     /locus_tag="PLD67_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOT21160.1"
                     /translation="MQYVKLEDLVSYTGIEPVYYPEDVQSRIYTPDIVRPGLQMAGYF
                     EWFSFERVQVMGKAEILYIETLDEEVKKERLDKFFSFPIPALIVANEMEVDDLIIYYA
                     KKYKRPVFKSNERTDKLVNIMINFLEEELAEETTLHGVCLEVFGVGVLLRGKSSVGKS
                     ETALELINRGHRLVADDAVIIKKVEGGLKATCPELTRHLMEIRGIGILDIQHLFGVGS
                     IRIEQFVELVVDLEEWDENKEYDRIGKDEEYTEILGVKVSTVIIPVRPGRNTSAIIEI
                     AAKNYRQKLLGFNPLDTYNKKFRNMIR"
     gene            complement(8263..>8553)
                     /locus_tag="PLD67_01135"
     CDS             complement(8263..>8553)
                     /locus_tag="PLD67_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOT21161.1"
                     /translation="IELRKTDIVYKLIYRIQEEAHRFAIEYHRNLRDKTLFKSELDNI
                     KNIGEKRKINLLKKFGSIENIKTKSINDLLSVPGMNTVAAESVYNYFHKEKN"
BASE COUNT         2384 a         1772 c         1306 g         3091 t
ORIGIN      
        1 caagagaatt cggtcgtgaa gtagctacac ctgacgaagc tcgtgtaata ctgggactta
       61 aataaagata cgtaacctat tttttgaaat tttaagagaa cggcaatccg ttctcttttt
      121 cttttaatgt gtgtaacttt aacagatata aaacatatat taatttagta agtatagagg
      181 atgtgtatgt atggacaaag aaagtcttat atttgaaggt ataatcagaa aattaaatat
      241 tgatgaagcc gatgacaaaa taatcgaaaa taaaacaaat actacataca aggcgcttat
      301 ttcatgcatc acaaccttgc cccccaagca atttgtactt ttgtcaacct tgataggcat
      361 tttattgata gatgacatcg atccgaaaga ccaaattata ttaggaaagt ttatcaacaa
      421 cattggtcaa acaatattaa cagcagccgc tcaggagcaa atcaacatta aaaaagaaaa
      481 ctaatgagtg ttgtaatacc acaataaatg aatgcacttt gtcacctgag cataaacgaa
      541 agatctcgtg agattcttca ctgcgttcag aatgacactt acctgtaatt aataacaatg
      601 acgatcaatc tctattcatc tctttccatt gctaattcca caagcttcac aatcaaatcc
      661 ttgtattcaa tgcctatttt tttaagcatt gaaggatatc tgcttaaatc cgtaagaccg
      721 ggaatagtat tgatttcatt taaatatatt tcattgtctg gagttaaaaa catatccact
      781 ctggaaagcc ctctgcagcc taaggctttg taagtattag cagcgatttc ttttgcctga
      841 gcctttaagt catcgctgat tcgtgcggga caatgaatct ttgaattatg ttgagaatac
      901 ttttcaacat aatcaaaaaa gtcattcttg gtttcaattt catcaacttc acctattaac
      961 agctcttcat ttcccactac agcacagccg atttcaaatc cttcaatatt tgactcgata
     1021 acaatttttt tgtcgtattc aaaggcttcc ataattcctt tttcaaattc gtcatagttt
     1081 ctcaccttgc ttataccgat ggaagaaccg ttgttggcag gcttgatgaa caagggaagg
     1141 gaaagctttt ctaaagcctc tttatataaa tttttaatat tcagggtttt atgatttaca
     1201 acggacataa aaggagcggt tttaattcct gacatttcgc atatcatatg tgtgtattcc
     1261 ttgtccatgg atacagctga tgaagtcata tcacaaccta caaaaggaat tcctgccatc
     1321 tggcacattc cctgaatggt gccgtcctcg ccgttcctgc cgtggagaac gggaaaaata
     1381 cagtctattt caattttttc gtaaactttg tctctttcgt taagtttaat caatccttta
     1441 aaatcgccca tactcaacaa tacaggactt aaattgccct gatgccatgt atcgttatta
     1501 attttgtccg tgctcccttc ataatgaagc cacttgcctt ctgttgttat ccctaccaat
     1561 gttacttcat acagctcttt gggaacattg tcgattatgg cagctgccga atgcaatgat
     1621 acggaatact catcagactt tcctccaaat aatatggcta attttatttt attcattttc
     1681 ccactccttt aatttatttg ctatatattc aagttttaaa aaccttgagc ccttaaccaa
     1741 aattgtacag tcttcaatta aatatttttt taaatatagg gcagcttcct ccttcgtatc
     1801 aaattcaact atacttttaa tatttgtatt ttccatggca gccttagcta taaattttgc
     1861 ttcttgtcct atggtaacaa gttcatcaat atgatggcgg gataaatctc tccctacctt
     1921 gtaatgaaca tcatggctca tttctctgta accgagcatg tcccccaata cggcaatttt
     1981 cttttttgaa ttgaataatt caaaaatatc taaggcagct ttaatgctta caggattaga
     2041 tttatagcag tcatttaaaa tattgcattt attaatattt ataacttcgt ttctaaggcc
     2101 tgttttttca attcttaaaa gtcccgactt aatttcttcc gggttcattc ctaattccag
     2161 agcagccaaa attgctgcca tagcatttaa agcttgatgc ttgcctaata tatcaatatg
     2221 cagctcatca aagcgctcat cgcttacctt gattgtaatg ccctctaagt cttgcttaaa
     2281 ttccttgagc catattgtat tgtttttatt taaaccgaat gtttgttttc gtatgctttt
     2341 tttgatttga gtatttttca cggtttcttc aatcaaagta tcgtcaccgt aataaataaa
     2401 taggccctta tctttaatac cattaacaat ttcaagtttt gctttggcta tttcttcaat
     2461 tgaaggaaaa ttatctatgt gtactaaacc gacacttgtt aatattgcaa tatccggttg
     2521 taccaaatca gctaaaaaat caatttcgcc ctttctttcc atgcccattt ctataacagc
     2581 cgcttgacag tcctcatcta aggaaagaag cgtaaacgga actccaattt ctgtgttata
     2641 gtttccaatg gttttttgtg ttttaaactt ttgagacagt attgcagcgg ttatgtcctt
     2701 tgtggatgtc tttccgttgc tgcctgtcac gcccactatt ttagtattta atttatttct
     2761 gtaatatctg gaaagctcct gtaagccttt taccgtatct tcaaccaaaa taacagcaat
     2821 atcatgagga ggatttggct catctttatt ccacaaggtt gccgcagctc ccttttttat
     2881 ggcatcttta acaaaggcgt gcccatttac ggtcatcccc tttatgggta tgaaaagact
     2941 gttttctgta acatttcttg aatcaatact tacacctttt atatgtttgt ccgaatcata
     3001 tgttcctgta cactgagcat ttaaaatgtc agctgcttct tttatattaa tactaatcat
     3061 caatagttcc tttctattta attacagtta aattatattg taaaaccttg aatttcgcaa
     3121 ggtgcaaaaa caattcctaa aaagaaaaaa atgtcatcct gacgaagaat atatgattct
     3181 tcactgtatc agaatgacac aagacatcaa catcattatt tgtaattatt ttttctatct
     3241 gttgcttctt gccttatttc tttacgcata ctttttaatg cttgatcgcg cctttcgttc
     3301 ttttcttcta attcttgtct ttgcttttca tcttcggtta ttttgatttt atcttttgtt
     3361 tccctgtaat ttcttattgt attatcaata ttaaactgaa ttctttcaac attgtctctt
     3421 ctgtcatcag gcttaggtct cttatccata tttgctcctt atgttaattt ttatttttat
     3481 tattgctaaa aataaattaa ttaacctgcc tttttttgtt atttgataat tatagctgtg
     3541 gacaaaccat caacctgatt atattcaaac gttaaaaaga agacaggata cctgtcttct
     3601 tttagtaaaa atgtcttagt cgtcttaata cttcatttca gccattctca catagctttc
     3661 atatctgtcc ttagcatctt tttctgcttt agcatataat tcttcagcaa gttcagggaa
     3721 tgaagttttc agtgatgcat atcttacttc gccttcaagg aattctctga atgacgcagt
     3781 aggttctttg gaatctaata tgaacggatt cttgccttct tctttaagaa gcgggttgaa
     3841 tctgtacaaa tgccagtaac cagcttcaac agctttcttt tctctgtcga cggtcttgcc
     3901 cattcctgct ttaattccat ggttgataca tggtgcgtaa gcaataatca atgatggtcc
     3961 ttggtagctt tctgcttctt ttattgcttt taagaattga ttcatgtttg caccgatagc
     4021 aacctgggcc acatatacat agccatatgt tgttgcaatc aatccaaggt cttttttctt
     4081 tactctctta cctgctgcag caaattttgc cactgatgcc gtaggagttg cttttgaaga
     4141 ctgtccgcct gtatttgaat aaacctctgt atcaagtaca agtacgttca catcttctcc
     4201 tgatgccaac acatggtcta agccgccata tccgatgtcg tatgcccaac cgtcaccacc
     4261 gattatccac tgtgatttct tagctagata gtctttttta tccaatatat ttttaattgc
     4321 ctgagcacat ttatcgcatg gctcaagtcc ttcaagaatt tcaagaactt ttgcgctcgc
     4381 tgcttttgat tcttcgccta attctctgtt tgcaatccat tcttgtattg cttcctttaa
     4441 ttcaggctta gcatccaatt ccaatagttc ttccattgac atttgaatac tgtctcttat
     4501 ttgtcttacg gctgttgcca ttccgtatcc gtattcggca ttgtcttcaa acaatgaatt
     4561 tgcccaagat ggtcctttgc cgcaagcatc ttttgtatat ggagttgatg gtgcggatgc
     4621 tccccaaatt gatgagcagc ctgttgcatt tgcaatcatc attctgtcgc cgaacaattg
     4681 agttatggtt tttacatatg cagtttctcc gcagcctgca catgctccgg aaaactcaaa
     4741 gtatggtttg cagaattgac ttcccttaac tgatacttta tccattaaat catctttgat
     4801 tgtcacttta tcaatagcat atttccagtt tggtacttct ctttctactt cttctgcaat
     4861 tggagtcatt accaaagctt tattgccttt tttacccggg cagatatctg cacagtttcc
     4921 gcaacccgta caatccaatg tgcttatttg aattctgtaa tccaaacctt ctaatccctt
     4981 gccaaccgct ttaatagtta caaaatcttt cggtgcattt gctttttcat tttcatcaag
     5041 caatgtaggt cttattacag aatgcggaca tatgaatgaa cactgattac actgtataca
     5101 gttttcagga atccatcttg gaactgatgt tgcaattcct ctcttttcat atgcagctgt
     5161 tccgtgaggg aaggttccgt cttctctgcc tgcaaatgta ctcactggta atttgtctcc
     5221 ctgctgtcta gtcataactt cagcaacatt ctttataaat tcaggttttt catcttcagt
     5281 gaaaggtact gccaacccgc tgtttgcttt attatcatca actgcatttt tccattcttc
     5341 aggtatatct attttaacaa gctctgtaat tcctttatca acagcagctc tattcatttc
     5401 aactactgtt gtaccttttt tgccatagga actttcaata gcatcataca aataattaac
     5461 tgcatcatct ataggaataa catctgctaa tttaaagaat gcagattgga ttatcatgtt
     5521 tgttctgtta ccgagtccta aatcttgagc tatttgagtt gcattgattg tatagaattt
     5581 aatatcattc tcagccatgt attttttata cttggcaggc aggtatgcat caagctcatc
     5641 ttttgaccaa atagtgttta ataagaatgt tccgcctttt ttcaatcctt caagcacatc
     5701 atatttatct acatatgatt gctgtgaaag cgaaacaaaa tctgcttcat ctattaagta
     5761 agttgatctg atgggtgatt ttccgaatct taagtgagat acagtaacac cgcctgattt
     5821 cttagagtca tatgagaaat aaccctgtgc atacatgtca gtatggtcac cgatgatttt
     5881 tattgcgtct ttgtttgcac caactgttcc gtcagagcca agtccccaga atttacatct
     5941 taccgttccc ggagctgata catttactac ttcaggaatt tccaaagatt taaatgtaac
     6001 gtcatcaaca ataccgattg tgaagccgtc ctttggttcg tcagctatta agttgttata
     6061 tactgcaaat atttgtgccg gagttgtgtc ttttgagcca agtccatatc ttccgcctac
     6121 tattacgggt gcatcttctt tgttgtagaa taatgattta acatccagat acaagggctc
     6181 accaaccgag ccgggttctt tggttctgtc taacactgct atttttttaa cagacttagg
     6241 cagaacatta aagaaatatt tttcagagaa aggtctgtat aagtgtactg caactaaacc
     6301 gactttctct cctttagcac tcaaataatc aataacttct ttaattgttt ctgttaccga
     6361 gccgatggca actattacac ggtctgcatc ttctgcaccg tagtaaacaa acggtttgta
     6421 ttctcttccg gtgagcttgc tgatttcctt catataatca ttaactatat caggaacaac
     6481 ttcgtaatat ttgtttgaag cttcctttgc ctggaagaaa atatccggat tttgagcggt
     6541 tcctcttgta acaggatgct caggatttag agctcttttt ctgaattcat caacagcatc
     6601 catgtccact aatttagcta aatcttcata atcaattact tcaatttttt ggatttcatg
     6661 ggatgttctg aatccatcaa agaaattcaa aaaaggtacc cttgatttaa tagcagtaag
     6721 atgtgctact gcagaaatat ccattacttc ctgtacgctt ccagacgcta agagtgcaaa
     6781 acctgtttgt cttgcagcca taacatctga atggtccccg aatattgata aggcgtgacc
     6841 agctaccgct cttgctgtaa cgtggaatac tccgggaagc aattctcctg caattttata
     6901 catgtttgga atcattaaaa gtagtccttg tgaagcagtg taggttgatg ttaatgctcc
     6961 tgcttgcaat gagccgtgaa cagcacccgc agcgcctgct tctgattcaa gctcggttac
     7021 tcttactgtc tgtccaaaaa tgtttttctg accatgtgcc gcccattcgt caataagctc
     7081 aggcattggt gatgaaggtg ttatggggaa gatggccgcg acttcggtaa atgcatatga
     7141 aatatatgct gcagcggtat ttccgtccat agttttcata acttttgaca tttgtatcct
     7201 cctataaatt tatttttaat tgtaaccaaa atattgtcat tttaccaata ctcttaaatt
     7261 atatcttaaa taattattta atgcaacact aaaaaataaa gttgcatttt taccgaatca
     7321 tatttcgaaa ttttttattg taagtatcaa gaggattaaa accaagaagt ttctgtctgt
     7381 aatttttggc agctatttca ataattgctg atgtatttct tcccggtctg accggtataa
     7441 taaccgtaga taccttaaca cctaatattt ccgtatactc ttcatccttg ccgattctgt
     7501 catattcctt gttttcatcc cactcttcca aatcaaccac caattctaca aactgctcaa
     7561 tcctgatgga gcctacgcca aacaaatgct gaatatcaag tattcctata ccccttattt
     7621 ccattaagtg tctggttaat tcagggcagg ttgctttaag ccctccctca acctttttta
     7681 ttattaccgc atcatcagcc acaagtctgt gccccctgtt tatcagctct aaggctgttt
     7741 cgcttttgcc gacactgctt ttccccctta ataatacccc aaccccgaat acttccaagc
     7801 aaacaccgtg caaagttgtt tcttcagcca attcttcttc aaggaagtta atcattatat
     7861 taactaattt atctgttctc tcattagatt taaaaacagg tcttttatat tttttcgcat
     7921 aataaataat aagatcatca acttccattt catttgccac aattaatgcc ggtataggaa
     7981 agctgaaaaa tttgtccagt ctttcttttt ttacttcttc atccaatgtt tcgatatata
     8041 aaatttctgc ttttcccatt acttgaactc tctcaaagga aaaccattca aaatatcctg
     8101 ccatttgaag acccggcctt actatatcag gtgtatatat tctggattga acatcctcag
     8161 gataataaac cggttctatt cccgtgtaac tgactaaatc ttcaagtttt acatactgca
     8221 tattgtacca tccctctata ttaagtaatc ataatctaat gactagtttt tttccttgtg
     8281 aaaataattg taaaccgatt ctgccgctac agtattcatt ccgggtaccg acaaaagatc
     8341 attaatactt ttagttttaa tattttcaat tgagccgaat tttttgagta aattaatttt
     8401 tcttttttct ccgatatttt taatattatc aagctctgat ttaaacagtg tcttgtccct
     8461 taaattcctg tggtactcaa ttgcaaatct atgagcttct tcctgaatcc tgtaaattaa
     8521 tttataaact atatcggtct ttctgagttc aat
//
LOCUS       DAOSOW010000020         8542 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_8225_length_8542_cov_3.483799, whole genome shotgun sequence.
ACCESSION   DAOSOW010000020 DAOSOW010000000
VERSION     DAOSOW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8542
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8225_length_8542_cov_3.483799"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLD67_01140"
     CDS             complement(<1..381)
                     /locus_tag="PLD67_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT21162.1"
                     /translation="MHYVVERKVNSDIKCGKKIIEEILGSIEGLIDPNTYFNTKLILN
                     ELMINSVVHGNCGDLNKMIYIKVYINESRIIVEVSDEGSGICYEHKNCGEYDYLECGR
                     GLMLVEGLSDKLSIEGNTVTCVQYL"
     gene            complement(480..1073)
                     /gene="yihA"
                     /locus_tag="PLD67_01145"
     CDS             complement(480..1073)
                     /gene="yihA"
                     /locus_tag="PLD67_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOT21163.1"
                     /translation="MIIRKAEFLISAVKKEQYPNTGVPEIAFAGKSNVGKSSMINALV
                     NRRNLAKTSSQPGKTQTLNFYNINDEFNFVDLPGYGYAKVSKAEQAKWADMIDLYLHS
                     RQQLKEVILLVDIRHEPGRNDLLMYEWIKSFGFKGYIIATKADKLSKSQQIKSLNNIK
                     KTLQIKDIGLIYQFSSTSKLGVEEIWILFESILNLHD"
     gene            complement(1124..3451)
                     /gene="lon"
                     /locus_tag="PLD67_01150"
     CDS             complement(1124..3451)
                     /gene="lon"
                     /locus_tag="PLD67_01150"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOT21164.1"
                     /translation="MIKNYKIERRNLPLIPIRGIGIFPNTVIHFDIGREKSINALEEA
                     MLEDSDIFLTVQKVADMESPKEDDFYEVGVICKIKQMIKMPGDNIRVLVEGINRAKIV
                     SVTQDDPYFEVALDEYIYDDSLPIDDNLEAMVRLTLNNFEEYALIYAKIAPDAVISLK
                     EIKNPDKLADVVASYIYLKTNQKQALLEIFDPYKRLESINKMLAKEIKVLQIENEINE
                     LVKKQVSEFQKDYYLKEQMRAIQKELGEDEGTDYEVEEYLQKISKANMPKDVKEKAEK
                     EINRLLKISPSSPDAGVIRTYVDWLLDLPWNKKTKDNTDLSRARKILAEDHYGLEEVK
                     ERILEYLAVKNIKKDMKGPILCFVGPPGVGKTSIAKSIARAMDRKYVRISLGGVRDEA
                     EIRGHRRTYIGAIPGRIISSIAKVKVNNPVFLLDEIDKLNSDFKGDPASALLEALDPE
                     QNNTFTDHYIEAPFSLQNVMFITTANTTSTIPGPLLDRMEVIEISGYTDIEKRNIAEK
                     YLIKKQLSEHGLKENQVKISTNAINEIIERYTRESGVRNLERNIGKLIRKAAVQIVEK
                     NKSTVSINNLNIKKYLGIPKYDYDIAYEEDQVGVVTGLAWTQVGGETLFVEVNTMKGD
                     GKLQLTGQLGDVMKESAMAALSYIKANAENFDIDNKIFKETDIHVHVPEGAIPKDGPS
                     AGITMATAIISALTKKPVRKDVAMTGEITITGRVLPIGGLKEKLLAAKRAGIKKVLLC
                     SKNKADVSKIDPKIIRSMEIVYADKIDDVVKEALR"
     gene            complement(3491..4750)
                     /gene="clpX"
                     /locus_tag="PLD67_01155"
     CDS             complement(3491..4750)
                     /gene="clpX"
                     /locus_tag="PLD67_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOT21165.1"
                     /translation="MSKVNEKHIKCSFCGKSQEQVRRLIAGPDVYICDECIELCYNIV
                     DEDIDLFEEYDFTELPKPKEMKAKLDEYVINQDNAKKALVVAVYNHYKRINYKTKDDG
                     VEIQKSNILLLGPTGSGKTYLAQTLAKLLNVPFAIADATSLTEAGYVGEDVENILLKL
                     LQAADFDVERAEKGIIYIDEVDKISRRSENPSITRDVSGEGVQQALLKILEGTVANVP
                     PQGGRKHPHQEFIQIDTTNILFILGGAFDGIEKIIQRRVGKKSIGFGADISGNNSMDT
                     DELLKQVQPQDLLKYGMIPEFVGRIPVIVALEKLDKNALISILTEPKNSLVKQYKELF
                     KMDNVELEIEKDALEEIAKKALEINTGARGLRGILESTMTEVMYEIPSRSDIKKCVIT
                     KDTIINKSEPKLVLTKKPVSDKKENVS"
     gene            complement(4762..5346)
                     /gene="clpP"
                     /locus_tag="PLD67_01160"
     CDS             complement(4762..5346)
                     /gene="clpP"
                     /locus_tag="PLD67_01160"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOT21166.1"
                     /translation="MTLIPYVIEQTGRGERSYDIFSRLLKDRIVILSDEVNDATASLI
                     VAQMLFLESEDPDKDIQFYINSPGGSITAGMAIYDTMQLIKPDVSTICVGMAASMGAF
                     LLLSGAKGKRFALPNSEIMIHQPLGGMRGQASDIKIHADRIIKIRDTLNKIISEKTGQ
                     PIAKVQQDTDRDNFMTAEEAKIYGIIDDIIAKRK"
     gene            complement(5416..6798)
                     /gene="tig"
                     /locus_tag="PLD67_01165"
     CDS             complement(5416..6798)
                     /gene="tig"
                     /locus_tag="PLD67_01165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOT21167.1"
                     /translation="MSSKLIKKEKNTVTLEFTVSQDEFDKAVNKAYLKVKNNINVQGF
                     RKGKAPRHIIEKKYGKSIFYDDALDIAVQEAYPKAVNELELDIIDSPKINVEKFEDGE
                     EIVFTAEVEVMPEVELPKYKGIEVVKTEIKVTDEDVERELKSIQEKNARIVEVADRPV
                     QKGDFLTIDYAGFVGEEQFEGGTAENQTLEIGSNSFIPGFEDQLIGKSKGDEVTVNVT
                     FPDDYHAENLKGKDAVFNVKIHEIKAKELPEMDDEFAKDVSEFDTLEELIEDTRKVLE
                     KKAADQSKISNDNKVITKIVNDTKLEVPEVLIQREIEHMGRDYEQQFRQQGFTGKEYD
                     ELINNFVNQYKESARGQAEFNVKAELVLEEIIKKEEITVSDEELQEEINKIAESYKVE
                     EDRLEGFKESLMQSSKGYIEETLKKRKVFDMLVENAVFVDAPETVESIEAHETINESE
                     KAEEPEEDKE"
     gene            complement(6948..7514)
                     /locus_tag="PLD67_01170"
     CDS             complement(6948..7514)
                     /locus_tag="PLD67_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT21168.1"
                     /translation="MKKLMLSGTVQIETRRFVLRKFQMSDAQDIYYNWLSDKDSAKYN
                     AWRVHTSIDETKEYLSEWTSLYKKDDYLHWAVVDKETKEVIGSVTVSNIKKWKKYCEV
                     GYTIAKKLWNQGIATEVLISILHYLTQEVGFEKVCAIHDIRNEASGRVMEKAGMKFVK
                     NKTHIFLNSDNIIMKCRLYEYRGMGTHL"
     gene            7759..8394
                     /locus_tag="PLD67_01175"
     CDS             7759..8394
                     /locus_tag="PLD67_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOT21169.1"
                     /translation="MPQEIYNASGIKIFGKRIKSLIYTTDLAIIKNNNADGVIAVYPF
                     TPQLAINQAIIDISPTPVFVGVGGGTTTGQRSIDIALNAELTGAYGVVLNAPTKNELI
                     SEMKERLDIPIIITIVSEKEDFESRIKAGVSIFNVSGGANTTNIVRQIREKHPYFPII
                     ATGGPTPESILETIRAGANIITYTPPPTGKIFSEMMEVYRHKITEELSTNK"
BASE COUNT         2349 a         1745 c         1185 g         3263 t
ORIGIN      
        1 taaatattgt acacaagtaa ccgtatttcc ttcaatgctt aacttgtcgg aaagtccttc
       61 caccagcatt aaacctcttc cgcattccaa ataatcatat tccccgcaat ttttatgctc
      121 atagcaaata cctgaacctt cgtctgaaac ctcaacaata attcttgatt cgttaatata
      181 gacttttata tatatcattt tatttaagtc tccacaatta ccgtggacga cactatttat
      241 catcagttca ttaagaataa gttttgtgtt gaaataggta ttggggtcaa tcagtccctc
      301 aatgcttcca agaatctctt cgattatctt cttgccgcat ttaatgtcgc tattaacttt
      361 tctttcaact acataatgca taataattcc tcctcgtcat aatataccct gttttaatga
      421 gcttaaacag caaaatatat tataacacaa aggtattctt attttttaca ggggtttgtc
      481 taatcatgta aatttaagat gctttcaaat agtatccata tttcttctac tccaagtttg
      541 gatgttgatg aaaattgata tattaaacct atatccttta tttgtaaggt ttttttaata
      601 ttattcaaac ttttaatctg ttgggactta gataatttat ctgcttttgt agctataata
      661 tatcctttga acccaaagct tttaatccat tcgtacatca gcaggtcgtt tctgcccggc
      721 tcatgcctta tatctacaag cagtataact tccttaagct gttgtctgct gtgcaagtat
      781 aaatcaatca tatctgccca tttagcttgt tctgcctttg atacttttgc atagccatat
      841 cccggaaggt ccacaaagtt gaattcatca tttatattgt aaaaattcaa ggtttgtgtt
      901 tttccgggct gtgagctggt tttcgcaaga tttctcctgt tgaccaaagc atttatcata
      961 gaagacttgc cgacatttga tttgcctgca aatgctattt caggaactcc agtattcgga
     1021 tactgctctt ttttaaccgc tgaaatcaaa aattcagctt ttctgataat catatatatc
     1081 ccttcttatt cttggccaaa accgttagcg gattttttat atcttatcgc aacgcttctt
     1141 tcaccacatc atctatttta tctgcatata caatttccat tgagcgaatt atttttgggt
     1201 caattttcga aacatccgcc ttgtttttag agcagagtaa aactttttta attcctgctc
     1261 tctttgcagc caaaagtttt tctttaaggc ctccgatggg aagaactctt cccgtaattg
     1321 taatttcacc ggtcatcgca acatcttttc tgacaggttt ctttgtcaaa gccgatatta
     1381 ttgctgttgc catggtaatt cctgcggaag gaccatcctt tggaattgca ccttcaggca
     1441 catgtacatg gatgtccgtt tctttaaata ttttattgtc aatatcgaaa ttttccgcat
     1501 tggcttttat atagcttaat gcagccattg cagattcctt cataacatcg cctaattgtc
     1561 ctgtaagctg tagtttcccg tcacccttca tggtattcac ttcaacaaac agtgtttctc
     1621 cgcctacctg agtccaagca agtcctgtta caactccaac ctggtcttcc tcataggcaa
     1681 tatcataatc atatttaggg atgcctaaat atttttttat gtttaaatta tttattgaaa
     1741 cagtgctttt gtttttttct actatttgaa ccgcagcttt tcttattaat ttacctatat
     1801 ttctttctaa gtttctgact cctgattctc ttgtatatct ttcgattatt tcatttattg
     1861 catttgttga aatttttacc tggttttctt taagtccatg ctcggaaagt tgttttttaa
     1921 ttaaatattt ttctgcaata tttctttttt caatatctgt gtatcctgat atttcaataa
     1981 cttccatcct gtccaaaaga ggaccgggaa tggttgaggt cgtgtttgcg gttgtaataa
     2041 acattacatt ttgcaaactg aaaggtgcct caatataatg gtcggtaaat gtattgtttt
     2101 gctcagggtc aagtgcttcg agcagtgcag aagccgggtc acctttaaaa tcactgttta
     2161 acttatctat ttcatccaat aaaaatacgg gattattcac ttttactttt gcaattgatg
     2221 atattattct tcccggtatt gcaccgatat atgttcttct gtgacccctg atttctgctt
     2281 cgtcccttac tcctcccaaa gaaattcgca catactttct atccatggcg cgagcaatgg
     2341 attttgcaat tgatgttttc cctactcccg gtggtcccac aaagcacagt attggtccct
     2401 tcatatcctt tttaatattc tttacagcta aatattccaa tattctttcc tttacttctt
     2461 ccaatccgta atggtcttca gcaagtattt ttctggctct gcttaaatcg gtattgtcct
     2521 ttgtcttttt attccaaggt aaatctaaaa gccaatcaac atacgttctt ataaccccgg
     2581 catccggtga agatggagat atttttaaaa gtctgttaat ttccttttct gctttttctt
     2641 ttacatcttt aggcatgttg gcctttgaga ttttttgaag atactcttca acttcataat
     2701 ccgtaccttc atcttcccct aattcttttt gaatagctct catctgctct tttaaataat
     2761 aatctttttg aaattcgctg acctgttttt taacaagctc gtttatttca ttttctatct
     2821 gcagcacctt aatttctttc gctaacattt tatttatgga ttcaagcctc ttataagggt
     2881 caaaaatttc cagaagagct tgtttttgat ttgttttcag ataaatatat gaagcaacaa
     2941 catctgcaag cttatcggga ttttttattt ctttaagtga tataacggca tccggtgcaa
     3001 tttttgcata aattaaagca tattcttcaa aattgtttaa tgtaagacga accattgcct
     3061 ccaaattatc atcaatagga agactgtcgt catatatata ctcatccaaa gcaacttcaa
     3121 aataggggtc atcctgggtt acagatacaa ttttagccct gtttatacct tcaacaagga
     3181 ctctgatatt gtctccgggc attttaatca tttgctttat tttacaaata acgcccactt
     3241 cataaaaatc atcttcctta ggcgactcca tgtccgcaac cttttgtacg gtcaaaaaaa
     3301 tatctgaatc ttcaagcatt gcttcttcca atgcatttat cgatttttcc cttcctatgt
     3361 caaaatgtat tacggtattg gggaaaattc ctatgcctct tatcggaatc agcggaaggt
     3421 ttcttctctc aattttataa ttcttaatca tactttctcc ttaaaaaact gcctgctaat
     3481 ggcaggcagt ctatgaaaca ttctctttct tatcagaaac aggcttcttt gttaatacta
     3541 atttcggctc acttttattt ataattgtgt ctttagtaat aacacatttc ttgatatcac
     3601 ttcttgatgg aatttcatac attacttcag tcattgtgct ttcaagaatt cctcttaagc
     3661 ctcttgctcc cgtattgatt tccaatgcct tcttagctat ttcttccaga gcatctttct
     3721 cgatttccaa ctctacatta tccattttaa ataattcttt gtattgtttg actaaggagt
     3781 tctttggttc cgtcagaatt gatattaaag catttttatc taatttttcc aaagctacaa
     3841 ttaccggtat tcttccgaca aattcaggaa tcattccata cttcaataaa tcctggggtt
     3901 gaacctgttt taacaattca tccgtatcca ttgagttgtt tcctgaaata tctgctccaa
     3961 aaccaatgct tttcttaccc actcttcttt gaattatttt ctcaatacca tcaaatgctc
     4021 ctccaagtat aaacaatata ttggtggtat ctatttgaat gaattcctga tgaggatgtt
     4081 ttcttcctcc ctggggcgga acattggcaa cagttccttc aagtatttta agcaatgcct
     4141 gctgtacacc ttcgccgctt acatcccttg ttattgaagg gttctctgaa cgtcttgaaa
     4201 ttttatcaac ttcatcgata tatattatac ccttttcggc tctttctaca tcaaagtccg
     4261 ctgcctgcaa taattttaaa aggatatttt caacatcttc gcctacatat cctgcttcgg
     4321 ttaaagatgt ggcatcagct atggcaaagg gtacatttaa taattttgcc aatgtttgtg
     4381 ccaaatatgt ttttccgctg cctgtaggtc ccaacaatag tatgttgctt ttttgaattt
     4441 ctactccgtc atctttagtt ttgtagttaa ttcttttata atgattgtat accgctacaa
     4501 ccaaagcttt ttttgcgtta tcctgattta ttacgtactc atccaatttt gccttcattt
     4561 cttttggttt tggcagttcc gtaaaatcat attcttcaaa taaatctata tcttcatcaa
     4621 caatgttata gcataactct atacattcat cacatatata aacatccgga ccggctataa
     4681 gtcttcttac ctgctcctga gacttgccgc aaaacgagca tttaatatgt ttttcattta
     4741 ctttgctcat tgaaacactc cttattttct tttagctata atatcatcta ttatgccata
     4801 gatcttagct tcctccgcag tcataaagtt atctctgtct gtatcctgtt gtacctttgc
     4861 tatcggttga cctgtttttt cgctaataat cttgtttaat gtatctctta tcttgataat
     4921 tctatcggca tgtattttta tgtctgaagc ttgccctctc atgcctccca aaggctggtg
     4981 aatcattatt tctgaatttg gaagagcaaa tctctttcct tttgctccgg acaagagcag
     5041 gaatgctccc atactggctg ccatcccgac gcatattgtt gaaacatcag gttttattaa
     5101 ctgcatggtg tcataaattg ccatgcctgc agtaatggag cctccgggtg aattgatata
     5161 aaattgtata tctttatccg ggtcttcaga ctccaaaaac aacatttgtg caactattaa
     5221 gcttgccgtc gcatcattaa cttcatcact aagtattaca attctatctt ttaaaagtct
     5281 tgaaaatata tcgtaagacc tttcacctct accggtctgt tcaattacat atggtattaa
     5341 tgtcatcttt attcctccta tattatattt atttctacct taattgtact taattttctt
     5401 aaatttatct taatcttatt ctttgtcttc ttccggctct tctgcttttt cgctttcatt
     5461 aattgtttca tgagcttcta ttgattcaac agtctcagga gcatcgacaa aaaccgcatt
     5521 ttcaacaagc atatcaaaga cttttctttt tttaagagtt tcttcaatat agcctttgct
     5581 tgactgcatt aaactttctt taaatccttc aagtctgtct tcttcaacct tgtatgactc
     5641 tgctatttta tttatttcct cttgtaattc ttcatctgaa actgttattt cttctttttt
     5701 aataatttct tctaaaacca attctgcctt tacattgaac tcagcctgac ctctggcact
     5761 ttccttatat tgattaacaa aattattaat caactcgtcg tattctttgc cggtaaatcc
     5821 ttgctgacgg aactgctgct cgtaatctct tcccatatgc tcaatttccc tctggattaa
     5881 aacttcggga acttctaact ttgtatcatt tacaattttt gttataactt tgttgtcgtt
     5941 tgagatttta gattggtcgg cagccttttt ttccaatact tttctggtat cctctatcag
     6001 ctcctccaat gtatcaaatt cacttacatc cttagcaaat tcatcatcca tttcaggaag
     6061 ttcctttgct ttgatttcat gtattttaac attgaaaact gcatccttgc cttttaaatt
     6121 ctctgcatga taatcatcag ggaatgttac attaactgtt acctcatcac cttttgactt
     6181 gcctatcaat tggtcttcaa accccggtat aaaagaattc gagccgattt ccaatgtttg
     6241 attttcggct gtaccgcctt caaattgttc ttctccgaca aaaccggcat aatcaattgt
     6301 taaaaagtcg cctttttgaa ctggtctgtc cgcaacttca actattctcg catttttttc
     6361 ctgtattgat tttaattctc tttctacatc ttcgtcagtt acttttattt ctgtctttac
     6421 aacctcaatt cctttgtact tcggcagttc aacttccggc attacttcaa cttcggccgt
     6481 aaatactatt tcttcaccat cttcaaattt ttcaacattt attttaggac tgtctataat
     6541 gtctaattct aattcattaa cagctttcgg atatgcttcc tgaacagcaa tatccaaagc
     6601 atcatcataa aaaatgctct ttccatattt tttttctata atatgtcttg gagcttttcc
     6661 ctttctgaat ccctgaacat ttatattatt cttaaccttt aaatatgctt tattcacagc
     6721 cttatcaaac tcatcttgag atactgtaaa ttctaatgta actgtatttt tctctttttt
     6781 tattaatttt gaactcattt agccctccgt tatccttaag tttctatgta tcattcaatt
     6841 attgacctta gattcatgaa ttaaccatta tattataaca taaaacatct ttaaagcaac
     6901 ctttattttt ggggacattc ctcgaacttg caaagacttt ccttatacta gaggtgtgtc
     6961 cccatacctc tgtactcata taatctgcat ttcattatta tattatcgct gtttaaaaaa
     7021 atgtgagttt tattttttac aaatttcatt cctgcctttt ccataactct ccctgatgct
     7081 tcatttctaa tatcatgaat tgcacaaact ttttcaaatc caacttcttg tgttaaataa
     7141 tgcagtatac tgattaatac ttccgtagca attccttgat tccatagttt ttttgctata
     7201 gtgtaaccta cttcacaata ttttttccac tttttaatgt ttgatacggt aactgatccg
     7261 attacttctt ttgtttcctt gtccacaaca gcccagtgca aataatcatc ttttttataa
     7321 aggctcgtcc attccgatag atattctttt gtttcatcaa tagacgtatg aaccctccat
     7381 gcattatatt tagctgaatc cttgtcgctt aaccaattat aataaatatc ttgtgcatcg
     7441 gacatttgga atttcctaag tacaaatctt cttgtttcaa tctgaacagt ccctgaaagc
     7501 attaattttt tcaaaatgac ctccgctgat tgtactactt gtattatacc ttttatttat
     7561 aaaattgcat agctttattt ttatttgtgt tgaattttat taataaaaaa agtataataa
     7621 tctaaaaagg agtcgtatat ggataataaa gaaattatta ccgataatta cagtgaagcc
     7681 gtaaaatcaa acactgagat taaggataaa aagtttgtac cgaccattga atccgttcta
     7741 aaaaagaaac ccatacagat gccgcaagaa atatacaatg ccagcggtat aaaaattttt
     7801 gggaaaagaa taaagtcctt aatctataca actgatttag caattataaa gaataataat
     7861 gcagacgggg taattgcagt atatcctttt actcctcaat tggcaattaa ccaggcaatt
     7921 atagatatat ctcctacgcc tgtgtttgta ggtgtcggag gaggtacaac aacaggacag
     7981 agatcaattg atattgcttt aaatgccgaa ttgacgggag cctatggggt agtattgaat
     8041 gcgcctacta agaatgaact gatatccgaa atgaaagaga gattggatat accgattata
     8101 attacaatag tgtccgaaaa agaggatttc gaatcaagaa taaaggcagg agtatccatt
     8161 ttcaacgttt caggaggagc aaatacaaca aatattgtaa gacaaatacg agaaaaacac
     8221 ccatattttc cgattattgc aacgggtggg ccgacacctg aaagtatatt ggaaaccata
     8281 agagcaggtg caaacattat tacttacact ccgcctccaa ccggtaaaat tttctccgaa
     8341 atgatggagg tatacaggca taaaatcaca gaagaattga gcacaaataa ataaaattta
     8401 aagaacctcc taatttaatt atcgggatgg ttctttattt attattacat ctttttaagt
     8461 acttctctat tgtcttctat tatgttgttt atttctttga ttttttcttg aagcttggac
     8521 aatatttcat cgctgtaatt at
//
LOCUS       DAOSOW010000021         8512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_8263_length_8512_cov_3.989949, whole genome shotgun sequence.
ACCESSION   DAOSOW010000021 DAOSOW010000000
VERSION     DAOSOW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8512
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8263_length_8512_cov_3.989949"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..901
                     /locus_tag="PLD67_01180"
     CDS             158..901
                     /locus_tag="PLD67_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOT21170.1"
                     /translation="MWERAELKARAKQVLKENYWKSFLISLVLLLSGADSRGGGSSGR
                     GAGERIGEWIDYSEYLIIIIAVVILVIAFRVLIGYALEIGSRKFFVQSAQYKNDKGCY
                     NFAFDTGNFKGIISTMFLRDIYNFLWTLLLIIPGIIKSYSYRMVPYILADNPNFGADN
                     AITLSRKMMDGNKFALFVLELSFLGWFLLGLLAFGVGILFVNPYVYATEAQLYLVLRD
                     TAIDQGYCTAEDLNLYNDFVKHDGYYWEE"
     gene            953..1504
                     /gene="yyaC"
                     /locus_tag="PLD67_01185"
     CDS             953..1504
                     /gene="yyaC"
                     /locus_tag="PLD67_01185"
                     /inference="COORDINATES: protein motif:HMM:NF018564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HOT21171.1"
                     /translation="MNPIEVHYKNSFAKIKIGNFLAEHMNNAAIVCIGTDKCIIDSLG
                     PLTGTLLMKENIKIDVYGTLEKPVHAMNINEYIKNLRSKNYDCIIAVDACLSNKKSQG
                     IIEVIEGSITPGKGIGKFLPEIGDISIIGIVNSSDKEFHDLVQDTRLSLIYEMAETIC
                     GGILNACSMNISESFDQASISSV"
     gene            complement(1570..2937)
                     /locus_tag="PLD67_01190"
     CDS             complement(1570..2937)
                     /locus_tag="PLD67_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOT21172.1"
                     /translation="MKKRISLLIVILMVILSFTSALAIEIDEDKFDQDIEFMKKVIYF
                     VLDKYQYEVSQEDIINGLYDGFFSVLDDYSTYYTPKEYQALLEDTAGEFSGIGVQIMD
                     INGQVVVVTPLPGSPAIEAGIKAGDIIKTVDHYDITGVTSSQASLLIRGEEGTPVKIG
                     ILRGKENLTFNLIRRAIVINNVEGKILEDNIGYLKVTGFSDNTAELVEKELELFDEKN
                     VKKIIIDMRNNGGGTLQSAVDLLNLFVTEGPVVYVKYAAGKEDIYESKLKDKKYEIAV
                     LINEGSASATEIFAGAVKYKNEGIIIGTNSYGKGVVQSLYHLTDGSGVKFTTAEYFSV
                     DRIPVHKIGITPDIAVENEKTDISEYPKFSKEQKPELGNVSLDVLAAEMILMELGYEI
                     NQPDGVYDIQSFEQVRKFQEDNLLYPYGTIDFATQDALYNALIKHMESNLEDLQLKAA
                     IEALK"
     gene            complement(3023..4288)
                     /locus_tag="PLD67_01195"
     CDS             complement(3023..4288)
                     /locus_tag="PLD67_01195"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015295973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOT21173.1"
                     /translation="MKTNLLTNILKNQVTPALGCTEPGAVAYAVARAKEILGSEVNKL
                     NLEVDKNILKNGFSVGIPGTEEHGIIFAAALSLVIGKSEYVLEVLKDVDKESIDRALE
                     IVDSNIIDLRLNSHVQGLYIKAEAIGEKDKATVIIRDAHTNIVFERKNDLVLFDRGDI
                     SEKKANNIKFEIKSFTIDELIDYVNNVPIENISFIQNGIDMNLKIADAGLKEAPGVGL
                     SNYLYKKAGDVFTMAKAYTAAASEARMSGYPLPVMSSAGSGNHGLVAVIPISYIGREN
                     KISNERILRSVTLSHLITIFVKVYLGALSPVCGCGVAAGVGCAAGLTYMLGGDNEQIK
                     GSINNMIAGISGMLCDGAKLGCAYKLSISVDAAVDAAKMALEKIYIPEDNGILGCTAE
                     KTIQNLAQVSNIGMDHTDEVILDVIINKC"
     gene            4644..6371
                     /locus_tag="PLD67_01200"
     CDS             4644..6371
                     /locus_tag="PLD67_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT21174.1"
                     /translation="MNILMNIKHELQHIIEAMHAVTNVDITIVDESLQRIATTVNEEY
                     SLGAKAPIYSAFHKCIMTGEQYFLENPKENPICFDCENVQSCKELVELCIPIKFNGKI
                     LGVIGMCAYDEKARQNLLMNRESFMNLESQLVNIISTVISEKKIMKMLEYSSSELMTL
                     INSLNQGIIIINNNYEIINTNNYANEKLNINTSKIPDLWNLLSEKLYNNLISKNFIGE
                     VGPVKLNNTEFVINAYPIMVKDEREGTVLVLSDFNKMKESVIESNRKKDIVTFDDIVG
                     ESEALKQARREAIQVADSDASILILGETGTGKEVFARAIHNLSYRKNEVFMAINCGAI
                     PENLIESELFGYEKGSFTGASVTGNPGKFEICKDGTLFLDEIGELPLSMQVKLNRALE
                     EKEILRVGGNNPIKVNPRIISATHCSLASMVENNLFRDDLYYRLNVVPIHLPPLRERG
                     YDVIILSRYFLNRFNNVYKKQLQGFTPEAERLLMGYKFPGNIRELRNLIECAVIFEEN
                     SYIGAENLRKKMGTSTSENLKLSELTRLYEKSVINSKIQELGGDLESKKKAAEHLGIS
                     IATLYRKLE"
     gene            6476..7438
                     /locus_tag="PLD67_01205"
     CDS             6476..7438
                     /locus_tag="PLD67_01205"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HOT21175.1"
                     /translation="MNRNLKTSNITESAMITGILVIIAYLSSFITLLMFFYPTPAIIL
                     GKRKGLKYSLLSLIASDLIISMLLGLQTGLVFLILYTPLALALTYGVCRNEEANKTIL
                     FGSAAYMISFVLYILLLNYIMGINFIERIAEIYEQSFETTRGLFNNIPDQLRTEQIEQ
                     YINDIEKMAPMMNYIVTNVFPAVLIVASVVTSYINYMVASKFAVRFSINVKQHEGIAY
                     FSFPRNFMISMAGLLLLSFLLRLLNINVGIIQMNLFIIVFMAMLLQGVAVLKFFVDKS
                     RMGKFARNLLLVFIVLMSINFSIAYAVIGLIDLTVNLRKINRAV"
     gene            7453..>8512
                     /locus_tag="PLD67_01210"
     CDS             7453..>8512
                     /locus_tag="PLD67_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21176.1"
                     /translation="MDKKTPVFLKDDSFIYFVVIVLLIIIFLIESMFIYAASAGLVLV
                     IITAFTLRKQILRSRELKNYLINYTKNLENLSINSFYYLPLPICIISLNGKVFWYNNK
                     FKELFPENIDIIENVDEFVGGFPLNSMDGNKEGTLSNIEVPETGRSFNVLYYELEEGR
                     FGEGKSYVCYWNENTAFVNLRNKYNDERPITMLIQIDNYEEISEKLELGEKSSMTAQT
                     EKILNSYAAEMNGFVLKYDNYKFLMMIENKYLETLEAKKFSMLDDVKSIKGTNDFNFT
                     LSIGTGAAAKNLTQLMEYSKGALDVALGRGGDQAVVKRVNSVKFYGGKSKAVEKRTKV
                     RARIISYALRQIIDQSSNV"
BASE COUNT         2770 a         1379 c         1550 g         2813 t
ORIGIN      
        1 ctgttgttgg tatataccgc cttgtcattt tatatatagg tgacaagggg acgggggtgc
       61 tgtcatcttt gctagggaat ttatgtatat gacaataccc ccgtcccctt gtcagaaatt
      121 taatataatt aatttaaaaa attaacaagg agggaatatg tgggaaaggg cagagcttaa
      181 ggctagagca aaacaagtat taaaggaaaa ttattggaag tcatttttaa ttagtttagt
      241 tttactatta tcaggtgcag atagccgcgg cggagggtca tccggcagag gtgccgggga
      301 aagaatcggc gaatggattg attattctga atatttgata attattattg ccgttgtaat
      361 tcttgtaatt gctttcagag tgttaatagg ttatgcattg gaaataggct caagaaaatt
      421 ctttgtacag tcggctcaat ataagaacga taagggctgc tataatttcg cttttgatac
      481 agggaatttt aaaggtataa tttcaacaat gtttttaagg gatatatata actttctttg
      541 gacattgtta ttgataatac cgggaataat aaaatcttat tcatatcgaa tggttcccta
      601 cattttggct gacaatccta attttggtgc agataatgcc ataaccttaa gcaggaaaat
      661 gatggacgga aataaatttg cattatttgt cttggaattg tcttttcttg gttggttctt
      721 actcggtttg ttagcttttg gagttggaat tctatttgta aatccttatg tctatgcaac
      781 tgaggctcag ctttatctgg tactcaggga tacagccata gaccaaggat actgcactgc
      841 cgaagattta aatctttata atgattttgt aaaacatgat ggttattatt gggaagaata
      901 aagcataaaa caccccctgt gtgtatatac taacacatag ggggtgtttc ctttgaatcc
      961 aattgaagta cattataaga acagctttgc aaaaattaaa ataggaaatt ttttggcaga
     1021 gcatatgaat aatgcagcaa tagtatgtat aggaacggat aaatgcataa tagacagctt
     1081 aggaccctta acaggtactt tactgatgaa agaaaatata aaaattgatg tttacggaac
     1141 tttggaaaaa cctgttcacg caatgaatat taacgagtat ataaaaaact taagaagcaa
     1201 gaattatgac tgcataatag cggtggatgc ctgcttaagc aataagaaaa gtcaaggaat
     1261 tatagaagtt attgagggct caatcacacc gggaaagggt atagggaaat tccttcctga
     1321 aatcggggat atatctataa tagggattgt aaattccagc gataaggagt ttcacgattt
     1381 ggttcaggat acaagacttt ctcttattta tgagatggca gaaacgatct gcggaggaat
     1441 attaaatgct tgcagcatga atatatcaga aagttttgac caggcttcga taagcagtgt
     1501 ataaggtatg gggacacacc tcttctttag ggaaagtctt tgaagtaaga ggaatgtccc
     1561 cagtttttgt tattttaatg cttcgatggc agccttcagc tgtaaatctt ctaaattgct
     1621 ttccatgtgt tttatcaatg cattgtatag tgcatcctga gtagcaaaat caattgttcc
     1681 gtatgggtat aataaattgt cttcctggaa ttttctaact tgttcaaatg attgtatgtc
     1741 gtaaactcca tccggttggt ttatttcata acctaattcc ataagtatca tttcagcagc
     1801 taatacatca agactgacat ttcccaactc gggtttttgt tccttgctga acttcggata
     1861 ttcagaaata tcggtttttt cgttttctac tgcaatatcc ggtgttattc ctattttatg
     1921 tacgggtatt ctatctactg aaaaatattc cgctgtggta aattttactc cgcttccatc
     1981 ggttaaatga tacagagact gaacaactcc ttttccgtaa gagtttgttc ctataataat
     2041 tccttcattt ttatacttta ctgcccctgc aaatatttct gttgcgctgg cacttccttc
     2101 gtttatcaat acggcaattt catatttttt atctttaagt ttgctttcat aaatatcttc
     2161 cttgccggct gcatatttca catataccac aggtccttcc gtaacaaata aattcaacaa
     2221 gtcaaccgca gactgcaatg ttccaccgcc attgttacgc atatctatta tgattttttt
     2281 tacatttttt tcatcgaata attctaattc tttttctaca agttccgctg tgttgtcgga
     2341 aaaaccggta acttttaaat atcctatatt atcttccaat atttttcctt caacattatt
     2401 tattactata gccctcctta ttaaattaaa ggtcagattt tcctttccgc gtaaaattcc
     2461 aattttaaca ggagtacctt cttctcccct tataagaaga gatgcctgac ttgaagtaac
     2521 tccggtaatg tcataatggt caacagtctt tattatatca cctgctttaa ttcctgcttc
     2581 aattgcaggt gagccgggca gaggagttac taccacaact tgtccgttta tatccattat
     2641 ttgaacgcct atgcctgaaa attctcctgc tgtgtcttca agcagagcct ggtattcctt
     2701 gggtgtatag taagtgctgt agtcatccaa aacactgaaa aacccgtcat aaagaccatt
     2761 tataatatct tcctggctta cttcgtactg atatttgtca agtacaaaat atattacttt
     2821 tttcataaat tcaatatctt ggtcaaattt atcttcatcg atttctattg ccaatgcaga
     2881 agtaaatgat aaaattacca ttaaaattac aattaacaag ctgattcttt ttttcataaa
     2941 gcacctcgtt tctttagatt tatcataggg tggacacacc gcgaacgaat atgtttctta
     3001 actatgagtg gacataccta tgttagcact tattaattat aacatcaagt ataacttcgt
     3061 ctgtatggtc catgcctata tttgaaacct gtgctaaatt ttgaattgtt ttttctgctg
     3121 tacatcctaa gataccgtta tcttccggaa tatatatttt ttccaaggcc atttttgcag
     3181 catcaacagc ggcatcaact gatattgaca gtttgtaggc gcagcctaac tttgccccgt
     3241 cacagagcat gccggaaatt cccgctatca tattgtttat tgagccttta atctgctcat
     3301 tgtctcctcc taacatgtat gtgagacctg ctgcacaacc aactcctgct gcaactccgc
     3361 agccgcaaac cggcgaaagt gctcctaaat atacttttac aaaaattgta atcaaatgac
     3421 ttaaggttac agagcgcaaa attctttcgt ttgaaatttt attttcccga cctatatatg
     3481 atatgggaat tactgctacc aaaccgtgat tgcctgaacc ggcactgctc attaccggta
     3541 aagggtatcc ggacattctt gcttccgaag cggcagcagt atatgcctta gccattgtaa
     3601 aaacatcccc tgctttttta tacaggtaat tgctcaaacc tacaccggga gcttctttta
     3661 atcctgcatc ggcaattttt aaattcatat cgattccgtt ttgaataaaa gaaatatttt
     3721 ctatgggaac attatttaca taatcaataa gttcatcaat tgtaaagctt tttatttcaa
     3781 attttatgtt atttgctttt ttttcggaaa tatcacccct gtcaaacaaa acaagatcat
     3841 tttttctttc aaaaacaata ttcgtatgag catcccgaat gataacagtt gccttatctt
     3901 tctccccgat tgcttctgct tttatataca atccttgaac atgagaattg agtcttaggt
     3961 ctataatatt tgaatcgact atttccaatg ccctatcgat gctttcctta tcgacatctt
     4021 tgagaacttc cagtacgtat tctgatttgc ctattactaa tgagagagca gccgcaaata
     4081 ttataccgtg ctcctctgtc cccgggatac ctacgctgaa tccgtttttt aatatgtttt
     4141 tatcaacctc tagattaagc ttattaactt cagagcctaa tatttctttt gctcttgcaa
     4201 cagcataagc aactgctccg ggctcagtgc atcctaatgc gggagtaacc tgatttttca
     4261 atatatttgt taatagatta gtcttcatat gcatctcctc ttctatttta tttaacctga
     4321 tactgcaagt acaagtgggt ttctcgtctt taataattta tatgcatgaa tcatgccaat
     4381 gcctaatttt aagaattgat tgaaaaatag acctttagtc aaagaatgat ctaaaaaaat
     4441 tatcaaaaat gataaataaa ttataaagga agttattagt ttgcataatt tgaatcttac
     4501 aaacgtttta tcagttttgc gcaagaatta tcaaaaatga taaatatttt gaaaatttca
     4561 aattaaagta tagtattaat aagcatcttg tattataata tattataatg aggcaaatat
     4621 cctttaagcg gggtagaggt taaatgaata ttttaatgaa tattaagcat gaacttcagc
     4681 atattattga agcaatgcat gcagtaacaa atgtagatat caccatagtt gatgaatctc
     4741 ttcagaggat tgcaaccact gttaatgagg aatacagcct tggagcaaag gctcctattt
     4801 attctgcctt ccacaaatgc attatgaccg gtgagcaata ttttctggaa aaccccaagg
     4861 aaaaccccat atgttttgat tgtgaaaatg ttcaaagctg caaggaatta gtcgagctat
     4921 gcattcccat taagtttaac ggaaaaatat taggtgtaat cggcatgtgt gcttatgatg
     4981 agaaagcaag acaaaatcta ttgatgaatc gtgaaagctt catgaattta gaaagccaat
     5041 tagttaatat aatatctacc gttatcagtg aaaagaagat tatgaagatg ctggaatatt
     5101 catcatctga attgatgacg ctgattaatt cattaaatca aggaattatt attataaata
     5161 ataattatga aattataaat accaacaatt atgctaacga aaaattaaat attaacacct
     5221 caaaaattcc cgatttgtgg aacctgcttt ctgaaaaact ttataataat ttaatttcca
     5281 agaattttat tggagaagtg ggtcctgtca agcttaataa cactgaattc gtaataaatg
     5341 cctaccccat catggtaaag gatgaaaggg aaggaacagt attggtattg tctgacttta
     5401 acaaaatgaa ggaatcggta attgaatcca acaggaagaa agacatagtt acatttgatg
     5461 atattgtagg cgaaagcgag gccttgaaac aggcaaggag agaagcaata caagtggccg
     5521 acagcgatgc ttctatttta atactcggtg aaacaggtac aggaaaggaa gtttttgctc
     5581 gagcaataca taatttaagc tacaggaaaa atgaagtatt catggctata aactgcggag
     5641 caattcctga aaatttaatt gaaagcgagc ttttcggata cgaaaagggc tcatttaccg
     5701 gtgccagtgt aaccggcaat ccgggcaaat ttgaaatttg caaggatggt acattgtttt
     5761 tggatgaaat aggtgagctt cctttatcaa tgcaggtaaa attaaacagg gcccttgaag
     5821 aaaaagaaat attaagagtg ggaggtaata atccgataaa ggttaatccc cgcataattt
     5881 ctgctactca ctgctccttg gcatctatgg ttgaaaataa tctattcaga gatgatttat
     5941 actacagact taatgtagtt cccattcatc ttcctccttt gagagaaaga ggatatgacg
     6001 tgattatttt atcaaggtat tttcttaacc gctttaacaa tgtctataaa aagcagctgc
     6061 aagggtttac gcctgaagca gagcggttat tgatgggata caagtttccc ggaaacataa
     6121 gagagcttag aaatttaatt gaatgtgcgg taatatttga ggaaaacagc tatataggag
     6181 cagaaaatct cagaaaaaaa atgggaacca gcacttccga aaacttaaaa ttgtcggaat
     6241 tgacaaggct gtacgaaaaa tctgtcataa acagtaaaat tcaagaatta ggcggtgatt
     6301 tagaatcaaa gaaaaaggct gccgagcatc ttggtataag tattgccact ttgtacagaa
     6361 agttggagta attacgtaaa gcttgaaata agtttttgac tttgattaga ttataggtta
     6421 aaatgttaaa acaacacaat gatatctctc tcgtatatag atatgtgagg taaagatgaa
     6481 cagaaactta aaaacatcaa acattacgga atcggcaatg ataaccggga tattggttat
     6541 aattgcttat ttgtcatctt ttattacatt attaatgttt ttctatccca cccctgcaat
     6601 tattttgggt aaaaggaagg ggcttaaata ttcactttta tcattaatag cttcggattt
     6661 gataatttcc atgcttttag gcttgcaaac gggacttgta tttttaattt tatatacacc
     6721 tttagctctt gcacttacat atggtgtctg cagaaatgaa gaagctaata aaacaatttt
     6781 gttcggttcg gctgcttata tgatttcatt tgttctttac atattacttt tgaattatat
     6841 aatgggcatt aattttatcg aaaggattgc agaaatatat gagcaaagct ttgaaacgac
     6901 aagaggatta tttaacaaca ttcctgacca acttagaact gagcaaattg agcagtatat
     6961 aaatgatatt gaaaaaatgg caccgatgat gaattacatt gtaacaaatg tatttcctgc
     7021 ggtattgata gtagcatcag tagtcacatc atatattaat tatatggttg caagtaaatt
     7081 tgcagtacgg ttttcaataa atgtaaaaca acatgaagga atagcttatt tttcgtttcc
     7141 aagaaatttt atgatttcta tggccggatt gctgctatta tcgtttttat tgcggttatt
     7201 gaatattaac gttggaatta tacagatgaa tctatttatc atagtcttta tggctatgct
     7261 tttacaaggg gttgcagttt taaaattttt tgtagataaa agtagaatgg gtaaatttgc
     7321 aagaaattta ttattggttt tcattgtact tatgagtata aatttcagca ttgcttatgc
     7381 agtaatcgga ttgattgatt taaccgtaaa tttaagaaaa attaacagag ccgtatagtt
     7441 gaaggaggca atatggataa gaaaactccg gtatttttaa aagatgacag ttttatatat
     7501 tttgtagtta tagtattatt gataataatt tttctaattg aaagtatgtt catttatgct
     7561 gcctcagctg gtttagtctt agttataata acagctttca cattaagaaa acaaattctg
     7621 cgcagtaggg aacttaagaa ttatctgatt aattacacga agaatttaga aaacttatca
     7681 ataaattctt tttattattt acccttgccc atatgcataa tcagtttaaa cggcaaagta
     7741 ttctggtata acaataagtt taaagagctg tttcctgaaa atatagatat aattgaaaat
     7801 gtagatgaat ttgtcggagg attcccgtta aactcaatgg atggaaacaa ggaaggcact
     7861 ttaagcaata tagaggttcc tgaaacgggc agaagtttca atgtattgta ttacgaactt
     7921 gaagaagggc gattcggaga aggaaaatcc tatgtttgct attggaatga gaatacggca
     7981 tttgtcaact tgaggaataa atataatgat gaacggccta ttactatgct gattcagata
     8041 gacaattatg aagaaatatc agaaaaatta gagctgggtg aaaaatcttc catgactgca
     8101 cagacagaaa aaattcttaa ctcttatgct gctgaaatga atgggtttgt gctgaaatat
     8161 gacaattata aatttttgat gatgattgaa aacaaatatc ttgaaacatt ggaagcaaag
     8221 aaattttcca tgcttgatga tgttaaatca ataaagggta ccaatgattt taacttcacg
     8281 ctcagcatag gtaccggtgc agcggctaag aatttaactc agcttatgga gtattccaaa
     8341 ggagcgcttg atgtagcttt gggaagagga ggcgaccaag cagttgttaa aagggtgaat
     8401 tccgttaaat tttacggcgg caaaagcaag gccgttgaaa aaaggacaaa agtcagggcc
     8461 aggattatat cttatgcatt gaggcaaata atcgaccaaa gctctaatgt ga
//
LOCUS       DAOSOW010000022         8498 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_8285_length_8498_cov_3.765131, whole genome shotgun sequence.
ACCESSION   DAOSOW010000022 DAOSOW010000000
VERSION     DAOSOW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8498
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8285_length_8498_cov_3.765131"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PLD67_01215"
     CDS             <1..447
                     /locus_tag="PLD67_01215"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOT21177.1"
                     /translation="GILFIIIENKNKGKPAKIKSFKELSYKTALITGFFQVLSLVPGT
                     SRSGATILGAIILGTSRNIAAEYSFFLSIPVMFGASALKLVKFGIDFTGLELAVLLTG
                     MTVAFIVSVIAIRFLLRYIKNNDFKAFGWYRIILGILVVGYFMFIS"
     gene            529..840
                     /locus_tag="PLD67_01220"
     CDS             529..840
                     /locus_tag="PLD67_01220"
                     /inference="COORDINATES: protein motif:HMM:NF041239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CC/Se motif family (seleno)protein"
                     /protein_id="HOT21178.1"
                     /translation="MFDENVIKITERAMKHLKKKGKLQITIEIPENRLSSESVLVPIP
                     EIIAKKPKAPENYQIINIGGIDVYISRTLVLPKNDVVIDLDTFLGIKLLRVSGFKSNE
                     G"
     gene            922..2580
                     /locus_tag="PLD67_01225"
     CDS             922..2580
                     /locus_tag="PLD67_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21179.1"
                     /translation="MRKASEKQRAWNIIWSVSENYSITPDITAYDEKGKADLYWNYII
                     GSVYKYYDYNLLREFFDTLRYDRDYIFYQKVLLLGLEGCIFEKDSKNRPVLAGLRFDY
                     GKKAYEKREEAEVFDEISAAYFSQVFGYDMKVRDNVKSLINELHFDINFNTEQVINKL
                     KQIINKYFEPEYSSEFYIKKIKDNMLLRFFGRKSPSSSLMLIDTDEGRTGTGKIASGW
                     FKFKEKSDNKEREYIKDYFGISAIAESKTKELEKILCDEAHESCHLHFTRGQFDENDD
                     AIFRQKQTSEQRDKNKQHYIKNYIRNTNNITKLTQRIKNAMIVNYTYSMKTESGKLVT
                     EKAWRTVLDDYKIFVRSIKDEIPDMSVDIMLDASASQLNRQEVIAEEGYIIAESLTRC
                     NIPVRIYSFCNMRDFTVMNLFRDYDEKNNNDKIFNYYASGFNRDGMALRTALHMMKDS
                     KYSHRILIVLSDGRPNDMLGVTSKGIFPLQKEYSDETGVGDTANEVRKGWKQGVSILC
                     IFTGRDDDIPSVKKIYGQKFTRILKLDKFAEMVGVLIEKELINK"
     gene            2725..4446
                     /locus_tag="PLD67_01230"
     CDS             2725..4446
                     /locus_tag="PLD67_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015009314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21180.1"
                     /translation="MKKLLPFMKKYIKFAVLSPILMILEVIADIAIPYLMSRIVDVGI
                     ANRDIEYVIKIGLIMIAAALLAMISGVISSHYGARAGFGFASEVRLKLYKKIQGFSFA
                     NLDKFTVASLITRLTNDCNTLGQVTMMSLRMAVRAPFMMIFALLMTIRINASLTKVFA
                     VSIPVLVIALIIILSKARPLFLKFQTRVDRVNAIIRENLTAIRVVKSFNRQSHEEKRF
                     KERNDSLMLTALKAITLIVFLMPVLNIIIYSTIIAVLWFGGKQVVAGSMGSGELISFI
                     TYITQILMSFMMMSFFFMQLLRASASVTRIAEIIDTESEIKEVKNPVKELADGSVTFK
                     NVCFCYPGSMEKTLKNINLHINSGEVLGIIGSTGSSKSTLVQLIPRLYDATEGSVIVG
                     NVDVKHYSIKTLRDNVAFVLQNNTLFSGTIRDNMKWGNENANDEDIIRALKLAQAWEF
                     VSTYEDGLDHKVEQGGDNFSGGQKQRLTIARALLKSPKIIILDDSTSAVDMATDAKIQ
                     RVFKEELNDVTTIIIAQRVSSIQHADRILVMNEGEIESIGKHEELLVSSPVYREIYES
                     QQKGVGV"
     gene            4443..6263
                     /locus_tag="PLD67_01235"
     CDS             4443..6263
                     /locus_tag="PLD67_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21181.1"
                     /translation="MKEKQLKPKDPKKTFLRLFSYFKYNIVLFFGGIFFIIVGSSAEI
                     GINGMISPVIDALIGDNDSGLFVKYLLIMVALVVISSIFQYIGNLSMAKLAQKTVYKI
                     REDMFSHMEKLPVSYFDKHSHGELMSTFTNDVDMLNQSLEQSASQILISFVTVVGTFT
                     VMIILSPILTAVVAAMLVVMLYSIKFVSKRSAKNYRMQQAALGDMNGYTEEMMSGQKV
                     VKVFNYEDRAIDTFSKKNEELRNASTLASTYGVMLMPIMGNLSFVMYAIISMFGAYLV
                     MINQLSIGNIASFLLYTRTVSRPITMVSNQLNSLFAALAGAERIFNVLDEKIETDEGD
                     VTLLKDCTGKKALCWMVPKGDGDYEKVPLKGFIEFKDVNFGYSPEKRVLNKIILYAKP
                     GQKIAFVGSTGAGKTTVTNLINRFYEINEGEILYDGIDIKRINKHDLRSTMSIVLQDV
                     HLFEGTVKENIRYGRLDATDDEVVEAAKLANAHYFIKHLPDGYDTVLSSDGQNLSQGE
                     RQLLSIARAAIADPIILILDEATSSVDTRTEKLIAEGMDKLMAGRTTFVIAHRLSTVR
                     NANAIMVIEHGEIIERGDHDELMSQKGRYYALNAGTAELS"
     gene            6322..7179
                     /locus_tag="PLD67_01240"
     CDS             6322..7179
                     /locus_tag="PLD67_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HOT21182.1"
                     /translation="MYGWMIYPKNTINNKFGNNAFDWMEESALKNGISIDIVFSDDLI
                     ILNNNSKTEFIYGGKSLKFPDFVIMRDYNYTISMQLEILGIRVINSTLSMFNSRNKAV
                     TAQLLVKNNVSTPKFLITKEKNFKFIHNYFDKRKFVMKENEGSQGKGVYLIENEEDYN
                     NVYHKIKDEYFCQEFIESSYGMDIRVYVLGDKVLGCVKRVSDSGFKSNYSLGGRVEKY
                     EMTDSIKEISLKAAKAIGLDFCGIDLLFAKDSFTVCEVNGNAGFRTITQVSDIDIPME
                     LFKWVKESL"
     gene            7358..>8498
                     /locus_tag="PLD67_01245"
     CDS             7358..>8498
                     /locus_tag="PLD67_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009060373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT21183.1"
                     /translation="MGIDIKDKLFNITEQYKEATELLISLGFDSLKDDNLRKVFGSSI
                     SLENALKSKKINSDEFVARLMERIEENKSAQKKFKDTIRITGVLPCPVKVPLMETFEK
                     WLSEQNFDFNLNYDLKAASMGIDWIKDEMKNSVNVPDLFISAGFDLFFDKNLLGKYKS
                     ENLFEDITGIQKYNDDFSEYNLEDPSCQYSMLGIVPAVFLVNKDELKGRKIPQSWEEL
                     MSGEFENNVSLPISDFDLFNALLLNIYRLYGEEGIRKLARIFQKNMHPSEMIKSHMKR
                     ERPAITIMPYFFTWMVREGSPMELVWPKDGAIISPIFMLTKKETKDKTKTIADFFASK
                     EAGEILSHKGKMPSVNPEVDNQIPKENKYIWLGWDFIKENDISNLI"
BASE COUNT         3033 a         1262 c         1731 g         2472 t
ORIGIN      
        1 ggtattttgt ttataattat tgaaaataaa aataaaggta agccggcaaa aataaagagt
       61 tttaaagagt tatcctataa aaccgcatta ataacaggct tctttcaggt attgtccctg
      121 gtacccggaa cctcacgctc cggggctacc atattaggag ctataatact tggcacatcc
      181 agaaatatag cagccgaata ttcatttttc ttatcaattc ctgtaatgtt cggagcaagt
      241 gcattaaaat tggtaaagtt cggaatagat ttcacgggtt tggaattagc agtattactg
      301 accggtatga cagttgcctt tattgtatct gtaatagcaa taagattctt gttaagatat
      361 attaaaaaca atgattttaa agcatttggt tggtatagaa ttattttggg tatattggta
      421 gtgggttatt tcatgtttat aagctaaaaa agttattgat atgaataagc tacgtgtctt
      481 tctgagggct tcagcccgaa gaatctcatc ttttataagg agtatgaaat gtttgatgaa
      541 aatgtaataa aaataaccga aagggcaatg aagcacctaa agaaaaaagg aaagcttcag
      601 attacaattg aaattccgga aaaccgctta agcagcgaaa gcgttcttgt gcccattccg
      661 gaaattattg ccaaaaagcc gaaagcacct gaaaactatc aaattatcaa tattggcgga
      721 atagacgtat atatatctag gacattggta ctgcctaaaa atgatgtagt tatagacttg
      781 gatacattct tgggtataaa acttctcagg gtatcgggat ttaaatcaaa cgaagggtaa
      841 caagcttcgt aataagatga tgacataaat taagtatgtc aaatgtcgga aagtatattg
      901 cataaaggaa gtaatcatca aatgagaaaa gcaagcgaaa aacaaagagc atggaatata
      961 atatggagtg tttcagagaa ttactccata acacctgata ttactgcgta cgatgaaaaa
     1021 ggtaaagctg atttatattg gaattatata attggttctg tttataaata ttatgactat
     1081 aaccttttga gagaattttt cgatactctc aggtatgaca gagactatat tttttatcag
     1141 aaagtattgc tgcttggact ggaaggatgc atctttgaaa aagatagtaa aaacagaccg
     1201 gtattagccg gtcttcgttt tgattacggc aaaaaggcgt atgaaaaaag agaagaggca
     1261 gaagtgtttg atgaaatttc agcagcatac ttcagccaag tattcggata tgatatgaaa
     1321 gtcagagata atgtaaagag cttaataaat gagctacatt ttgacataaa tttcaatacc
     1381 gagcaagtaa taaataaatt gaagcaaata attaataaat attttgagcc tgaatatagt
     1441 agtgaatttt atataaaaaa aataaaagat aatatgttgc tgcgtttctt cggaagaaaa
     1501 tccccttcaa gctccttgat gcttatagat accgatgaag ggagaaccgg aaccggcaaa
     1561 atagcttcag gatggtttaa attcaaagaa aagtcagata acaaggagag ggaatatatt
     1621 aaagattatt tcggaatttc cgcaattgcc gaatccaaaa cgaaagagtt ggaaaaaata
     1681 ctttgcgacg aagctcatga aagttgccat ctccatttta caagggggca atttgatgaa
     1741 aatgatgatg cgattttcag acagaagcaa acttctgagc aaagggataa aaataaacaa
     1801 cattatataa agaattatat caggaacaca aacaacataa caaaacttac acaaagaatt
     1861 aaaaatgcaa tgattgttaa ttacacatat tcaatgaaga ctgaatcagg aaagcttgtt
     1921 acggaaaaag cttggaggac tgtacttgat gactacaaaa ttttcgtcag aagtattaaa
     1981 gatgaaatac ctgatatgtc cgttgatatt atgcttgatg cttcagcatc ccagcttaat
     2041 cgtcaggaag ttatagcaga agagggctac ataattgcag aaagtctgac gcgctgcaat
     2101 attcccgtaa ggatatattc tttctgcaac atgagggatt tcactgttat gaatcttttc
     2161 cgcgattatg atgaaaaaaa caacaatgat aaaattttca actactatgc atcgggcttt
     2221 aaccgggacg gaatggccct tcgaactgct cttcatatga tgaaagattc aaaatacagc
     2281 catagaatac taattgttct ttcagacgga agaccaaatg atatgttggg cgtgacttca
     2341 aaaggtatat ttccgctgca gaaagaatat tcggatgaaa caggtgtagg cgatactgca
     2401 aatgaggtta ggaaaggctg gaaacaagga gtttcaattc tctgtatttt tacgggaaga
     2461 gatgatgata ttccatcagt caaaaaaata tacgggcaaa agttcactcg tatattgaag
     2521 ttggataaat tcgctgagat ggtaggagta ctcattgaaa aagaattaat caataaataa
     2581 agagatcctt cgcttacgct caggatgaca aatcttcgat ttatgtcatt gctatgaata
     2641 agctacatgt cattctgaga gctttagctc gaaatctcat tcgataggtt aacataatat
     2701 aaattatgta cagaggagaa cagattgaaa aagttattgc catttatgaa aaaatatata
     2761 aaatttgcgg tactaagccc catactcatg atattggagg ttattgccga tatagcaata
     2821 ccatatctga tgtcaagaat agtggatgta ggtattgcaa atcgtgacat tgaatatgtt
     2881 attaaaatcg gacttataat gattgcggca gctcttttag ccatgatttc tggtgtaata
     2941 agctcacatt acggtgcaag ggcaggtttt ggttttgcat cggaagtgag gctaaagcta
     3001 tacaagaaga tacagggctt ttcttttgca aatttggaca aatttacggt tgcatcgctt
     3061 atcacaaggc ttacaaatga ttgcaacaca ttggggcagg taacaatgat gagcttgaga
     3121 atggctgtaa gagctccctt tatgatgata tttgcacttt tgatgacaat aagaattaat
     3181 gcatctttga caaaggtatt tgcggtttcc atacctgtct tggttattgc attgattatt
     3241 attttatcaa aagcaagacc gctttttttg aagtttcaga cacgagttga cagggtaaat
     3301 gcaattatac gtgaaaattt aactgctatt cgggttgtaa agtcattcaa caggcagagt
     3361 catgaagaaa aaagatttaa agaaagaaat gactcattga tgcttactgc attaaaagcg
     3421 ataacgctta ttgtgttttt aatgcctgta ttaaatataa ttatttattc taccataata
     3481 gccgtgttgt ggtttggagg aaaacaagtt gttgcaggct caatgggaag cggagaatta
     3541 atatctttca ttacatatat aacacaaatt ttgatgtcct ttatgatgat gtcatttttc
     3601 ttcatgcagc ttctcagagc ttctgcttcc gtaacaagga ttgcagagat aatcgacacg
     3661 gagtcagaaa ttaaggaagt aaaaaatcct gtaaaggaat tggcagatgg ctctgttacc
     3721 ttcaaaaatg tatgtttttg ctatccagga agtatggaaa aaactttaaa gaatattaat
     3781 ttacatataa attcaggtga agtgttgggg attatcggct ccacaggctc atcaaaatca
     3841 accttggtcc agcttattcc aagactttac gatgcaacgg aaggcagcgt aattgtgggc
     3901 aatgtagatg taaagcatta cagtataaaa acattgcgtg ataatgtagc ctttgtgctg
     3961 cagaataaca cattattttc aggaaccatc cgcgacaata tgaaatgggg caatgaaaat
     4021 gcaaatgatg aagatattat tagagcactt aagttagctc aagcttggga atttgtatcc
     4081 acctatgagg acggattaga ccacaaggta gagcagggtg gagataattt ttccggaggt
     4141 cagaaacaaa ggcttacaat agctcgagct cttctcaaat ctcctaaaat tattatatta
     4201 gacgattcaa ccagtgctgt agatatggcg acagatgcaa aaattcagcg agttttcaag
     4261 gaagaattaa atgatgtaac cacaataata attgctcaaa gagtttcttc gattcaacat
     4321 gcagacagga ttttggtaat gaatgaagga gaaatagaat ctatagggaa acatgaagaa
     4381 ttgctggttt cttctcctgt atacagagaa atttatgaat ctcagcaaaa aggggtggga
     4441 gtatgaaaga aaaacagtta aaacctaaag atcctaaaaa aacatttcta agattgttct
     4501 cttattttaa atataatatt gtcttgtttt ttggaggtat tttctttata atagtcggct
     4561 cctcggctga aataggcata aacggtatga ttagtcccgt tattgatgcc ttaatcggag
     4621 acaatgacag cggactcttt gtaaagtacc ttcttattat ggtagcattg gtagtaatta
     4681 gctcaatttt tcaatatata ggtaacctgt caatggcgaa attggcacaa aaaacagttt
     4741 ataaaatacg tgaagacatg ttttctcata tggaaaaact tcctgtttca tattttgaca
     4801 aacactctca tggggaatta atgtccacct ttaccaatga tgtggatatg cttaaccaat
     4861 cacttgaaca aagtgcttca caaattctta tatcatttgt aacagttgtc ggaacattta
     4921 cggtgatgat tattttaagt ccgattttga cagccgtggt tgccgcaatg cttgttgtaa
     4981 tgctctattc aataaaattt gtaagcaaaa gatctgcaaa aaattatcgc atgcagcaag
     5041 ccgcattggg agatatgaac ggttatactg aagaaatgat gtcaggacaa aaggtagtta
     5101 aagtatttaa ctatgaggat agagctatcg acacgttcag taaaaagaat gaagaattaa
     5161 ggaatgcaag caccttggcc tctacatacg gtgttatgct gatgcctatt atgggtaatc
     5221 tgtcctttgt tatgtatgct ataatatcaa tgtttggggc atatttagta atgattaatc
     5281 aattgagtat cggcaatata gcatcattcc ttctatatac cagaacagta tcaagaccca
     5341 taacaatggt ttcaaatcaa cttaactcat tgtttgctgc cttggcagga gcagaaagaa
     5401 tattcaacgt attggatgaa aaaatcgaaa ctgatgaggg agacgtgacc ttgcttaaag
     5461 attgcacagg aaagaaagct ctttgctgga tggttccaaa gggtgacgga gattatgaga
     5521 aagtacctct caaaggtttt attgaattta aggatgtgaa ctttggatat tcgcctgaaa
     5581 aaagagtttt aaacaaaata attctctatg caaagcccgg acaaaagata gcttttgtag
     5641 gctcaaccgg agccggcaaa acaaccgtta caaatcttat aaacaggttt tatgaaataa
     5701 atgagggaga aatactctat gacggaatag atataaaaag gattaacaag catgatttgc
     5761 gaagcaccat gagcatagta ttacaggatg ttcatctgtt tgaaggaaca gtgaaggaaa
     5821 atatcaggta tggaagattg gatgctacgg atgatgaggt agtagaagca gcaaaacttg
     5881 caaatgcaca ttattttatt aagcatcttc ctgacggtta cgatacggta ctaagttcag
     5941 acggacaaaa tttatctcag ggagaaaggc agcttttgtc aattgcaagg gctgcaatag
     6001 ccgacccgat aattcttatt ctcgatgaag caacatcttc cgtagatact agaactgaaa
     6061 aattaatagc tgaaggaatg gataaattaa tggcaggaag aacaacattt gttatagccc
     6121 acagattgtc aacggttcgc aatgccaatg ccataatggt aatagaacat ggagaaatta
     6181 ttgagcgcgg cgaccatgat gaactgatgt cacaaaaagg acgatactat gcattgaatg
     6241 caggaacagc tgagttgagt taatgccaaa tggtgcctgg caccaattca tggcaccaat
     6301 tcatgtatac aggaggtaaa tatgtacgga tggatgattt atcccaagaa tactataaat
     6361 aataaatttg gaaacaatgc atttgattgg atggaagaaa gtgcattaaa aaacggtata
     6421 tctatcgata ttgtttttag cgatgattta atcattttaa ataacaatag caaaacagaa
     6481 ttcatatacg gcggtaaaag cttaaaattt cctgattttg taataatgag agactataat
     6541 tacactataa gcatgcagct tgaaatctta ggcataaggg ttattaattc cactctgtca
     6601 atgtttaact ccagaaacaa agcagttact gctcaacttt tggttaaaaa caatgtaagc
     6661 actcctaaat tcttaatcac aaaagaaaaa aactttaagt tcattcataa ttactttgac
     6721 aagaggaagt ttgtcatgaa agaaaacgaa ggctcccagg gcaagggagt ttatctcatt
     6781 gaaaacgaag aagattataa taatgtatac cataaaataa aggatgaata tttttgtcag
     6841 gaatttattg agtccagcta cggaatggat attcgcgtct atgtgctggg agacaaagta
     6901 ttgggatgtg taaaaagggt ttctgacagc ggctttaagt caaactattc attgggtgga
     6961 agagttgaaa aatatgaaat gacagattct atcaaagaaa tctctcttaa agccgccaaa
     7021 gccataggtc ttgacttttg cggcatagat ttgctttttg caaaggattc attcacagta
     7081 tgcgaagtga acggcaatgc aggatttaga acaattactc aggtatcaga tattgacata
     7141 cccatggaat tatttaaatg ggtgaaggaa agtttgtaac aaatgttaca ataattaagt
     7201 ttaaattgtg ttaaaataac ttatgaataa atcatgtatg aataacctgt gaagtgtcat
     7261 tctgagagct ttagctcgaa gaatctcatg tgatccttcg cttacgctca ggatgacata
     7321 aaggtgttag cttacaagat tgaaaaaagg agataccatg ggtatagata taaaagataa
     7381 attgttcaat ataaccgaac agtataaaga agctacagag cttttaatat ctttgggatt
     7441 tgacagtttg aaagatgaca acctgagaaa ggtgttcgga agctccatct cattggaaaa
     7501 tgcattaaaa tctaagaaaa ttaattcaga tgagtttgtt gcccgattaa tggaaagaat
     7561 tgaagaaaat aaaagtgctc agaaaaaatt taaagacaca attagaataa ccggagtgct
     7621 tccctgccct gttaaagttc ctttgatgga aacttttgaa aaatggctta gtgagcagaa
     7681 ttttgatttc aatttaaatt atgatttaaa agcagcatct atgggaattg actggataaa
     7741 agatgagatg aaaaactcgg taaatgttcc ggatttgttc atatctgcag gttttgactt
     7801 attttttgac aagaacctct taggaaagta caaatcagaa aatttatttg aagatataac
     7861 cggaattcaa aaatataacg atgatttttc ggaatataat ctagaagacc caagttgtca
     7921 gtattcaatg cttggaatag ttcctgccgt ttttctcgta aacaaggatg agttgaaggg
     7981 gagaaaaata ccccaaagct gggaagaatt aatgtcgggt gaatttgaaa acaatgtgag
     8041 ccttcccata agcgactttg atttattcaa tgctctattg ttgaatattt acaggctgta
     8101 cggagaagag ggcataagga aactggcaag aatattccaa aaaaatatgc atccttctga
     8161 aatgattaag tctcacatga aaagagaaag accggcaatt accataatgc cctatttctt
     8221 cacctggatg gtgagagaag gaagccctat ggaattggta tggccaaagg acggtgcaat
     8281 aataagccct attttcatgc ttacaaagaa ggaaacaaaa gataaaacaa agacaatagc
     8341 tgatttcttt gcttcaaagg aagcaggaga aattctgtct cacaagggta agatgccaag
     8401 cgtaaatcct gaagtagaca atcagatacc gaaggagaat aaatatattt ggcttggatg
     8461 ggattttata aaggaaaatg acatttcaaa cctaataa
//
LOCUS       DAOSOW010000023         8451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_8341_length_8451_cov_4.288471, whole genome shotgun sequence.
ACCESSION   DAOSOW010000023 DAOSOW010000000
VERSION     DAOSOW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8341_length_8451_cov_4.288471"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1695)
                     /locus_tag="PLD67_01250"
     CDS             complement(<1..1695)
                     /locus_tag="PLD67_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21184.1"
                     /translation="MKKESELKIIPILSKGFKRFFFISAAATILKVSASFAVPQVIRF
                     TVDHIIGDYPLNYIQLVMNTINNIGGIEFLKENLWLLTFFILVLAVITGVSDYIGKIT
                     LAKGTEGIIKNIRDKLFEHIQKLPYSWHIKNQTGDIIQRSTSDVEVVRNFISTQLVEI
                     FRVIMLVIISLILMFSMNFHLTAAVLVFIPVVVLYSTIFYGILTKKFLVADEAEGKLF
                     TAIQENLTGVRVVRAFGREKHEIEKFTERNNTFSELWVKLGYQLGYYWGIGDLVTGVL
                     LMVIVVYGTIFAINDRITLGEFLAFASYTVMISWPLRSFGRILSELSKAKVSLKRICE
                     ILKEPVEDDSPDSVTPDILKDIEFKNVTFKYDGETEVLKNVSFTIKAGSTFAILGGTG
                     SGKTTLMYLLNRLYDLPDHMGCITIGGVSIKNIKREHLRKNIGMVLQEPFLFSRTIKE
                     NIAIVEPDTSLDEIKKAASIAQIDESIEEFSKGYDTIVGERGVTLSGGQKQRVAIARM
                     LLQKTPIMVFDDSLSAVDADTDAKIRSSLKRRKGSSTTILISHRITTLMESDTILVL"
     gene            complement(1790..2836)
                     /locus_tag="PLD67_01255"
     CDS             complement(1790..2836)
                     /locus_tag="PLD67_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOT21185.1"
                     /translation="MAKKIISVILLILIIISLTGCSDKEPIRYQSEFIMLFDTVTRIV
                     GYSKSKEEFTEYAQIIYDNLKEYHQLYDIYNDYEGINNIKTINDNAGIKPVKVDKRII
                     DLIKFSKDMYEKTNGKTNIAFGAVLRIWHEYRTIGIEDPANAALPPEEELRIASEHTN
                     IEDIIIDEEQSTVFLRDPFMSLDVGAVAKGYATEQVSKIAKESGMNSALLSVGGNVRA
                     INKNVTSNEPWNVGIQNPDTEAEESVIKIVKLDDSSLVTSGDYERFYIVDGEKYNHII
                     NPDTLYPAKYFTAISIICKDSGLADALSTAVFCMPFEEGLEFINSLPEAEAMWIFSDK
                     TIKYSDNFESFIKK"
     gene            complement(2910..3995)
                     /locus_tag="PLD67_01260"
     CDS             complement(2910..3995)
                     /locus_tag="PLD67_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21186.1"
                     /translation="MKKLLAIVLILAMSLFAFVACTQDQPGETPKNEEPKTEEPKTEE
                     PKEEEPLAEGAVKTGLAVISSVEKSKDAGEKDGLAQTDSVIVAVTVDKDGKIVQTIID
                     TAQTKIEFSKEGKIIPDLNTVFKSKQELKEEYGMGKASSIEKEWYEQANALAEYVKGK
                     TVEEVKAIAVDETGVTTEADLVSSVTIKINPYKEAIEKAVANAENLGADASDKLGLGV
                     ITTIAKSKDATADAEGLAQAYSTYTVATFDADGKVTSSIIDASQANVKFDNTGKITTD
                     LAVAPLTKVELGEDYGMKKASAIGKEWYEQADAFADYVVGKTVEEIKGIAVDESGHAT
                     EEDLVSSVTVGIVDLKTIFEKAYNNSI"
     gene            complement(4200..4964)
                     /locus_tag="PLD67_01265"
     CDS             complement(4200..4964)
                     /locus_tag="PLD67_01265"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOT21187.1"
                     /translation="MKDITRVQSIDRAVAILDCFSEEKKELKLSEISERLGLNKSTVH
                     GIISTLKYHGFISQDEETQKYKLGIRFVQFGDLVINSMNIRNAAVPVIDAVCEKIEET
                     VHVAMLDGLDVVWIEKRECTKSIKTSTKIGARLPAYTTADGKIIICYQNKDKIKNYLP
                     KRIPQYTNNTITNKGEFIKKLEEMKKNGYAIDNEEYVEGLKCVAAPIFDHDGKVRFSL
                     STTGPAFRMNEERIKELIVIIKEAANEISHRIGYMK"
     gene            5255..5818
                     /locus_tag="PLD67_01270"
     CDS             5255..5818
                     /locus_tag="PLD67_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HOT21188.1"
                     /translation="MNITKEKAWELLKKYNKDEFHLQHADTVSFVMEYFAKKLGFEDE
                     AEFWGIVGILHDIDFEMYPDEHCVKMQEILSQNGVDEKIIKSAASHGYGLTDVTIKPE
                     HTMEKILFATDELTGLIGASALMRPSKSTLDMELKSLKKKFKSPGFAAGCSREAIKTG
                     AEMLGWELDYLLDETLKAMQEYEKARN"
     gene            complement(5844..6896)
                     /locus_tag="PLD67_01275"
     CDS             complement(5844..6896)
                     /locus_tag="PLD67_01275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOT21189.1"
                     /translation="MPPIAVEQIIKKRDKLYELDYMRFIACFAVMIVHITATGVSEYI
                     HGTFPYILMLTVNRSLKFTTPVFILLSGVTSFYSYSNKNREFKYMEFLKKRLAKVIVA
                     YLIWCIIYYAAYIYFGYYAIDIKFFVKSVLQGTMSYHFYFVIIIIQMYIVGPIFYFIL
                     KNSGKKVTILIFAAVITYLCAEFIRFDLSDRIFLKYIVFYMLGIYIAMEYDNYSLWIN
                     KNKLYIILGYIMMSIIYIVVSYYDSVASTFAWFIFSTMSLFFVYLIGLLMKDKLKNIY
                     GFIEVFGQSSYYVYLMHPLVLTIMILFAEKNFLSVTDKIILNTFTVIPVTVISCLTYT
                     FIKNKFKKKEKTLAVN"
     gene            7138..7380
                     /locus_tag="PLD67_01280"
     CDS             7138..7380
                     /locus_tag="PLD67_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009987400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT21190.1"
                     /translation="MVVREAVINRINEICDDRGIAVNKLANLSGITPSTVYSIFNSGR
                     KDIGIVLIKKICDGFDITLDEFFSSDLFKSLEQELL"
     gene            complement(7449..7694)
                     /locus_tag="PLD67_01285"
     CDS             complement(7449..7694)
                     /locus_tag="PLD67_01285"
                     /inference="COORDINATES: protein motif:HMM:NF022165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaL family protein"
                     /protein_id="HOT21191.1"
                     /translation="MNDVQRKISIKSIIESFKKNKSADEEMFKNIENAKREWEDAKNI
                     FENVSHPDLVDYAIYKVEAAEQKYIYLLKQFKSNNLT"
     gene            complement(7780..8244)
                     /locus_tag="PLD67_01290"
     CDS             complement(7780..8244)
                     /locus_tag="PLD67_01290"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOT21192.1"
                     /translation="MKSVFYYDTDIGRIGIAEDGNFITNVFFENEELEDIKINETPLI
                     KEAHKQIEEYLRGERKSFDLPISASGTEFQQRVWNALKTIPYGKTASYKDIAIAIGNE
                     KSCRAVGMANNRNPISIIIPCHRVIGKNGKMVGYGGGIEIKEYLLKLENNNL"
     gene            complement(8271..>8451)
                     /locus_tag="PLD67_01295"
     CDS             complement(8271..>8451)
                     /locus_tag="PLD67_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HOT21193.1"
                     /translation="TECYTVDELIGKQVLAVVNFPPRQIADFISEVLVLGVYTEKGVV
                     LIKPDQRVEKGAKLG"
BASE COUNT         2673 a         1556 c         1165 g         3057 t
ORIGIN      
        1 caagactaaa atagtatcag attccattaa agtggttatg cgatgggata ttaaaatagt
       61 tgttgaactg ccttttcttc ttttgagaga agaacgaatt tttgcatctg tgtcggcatc
      121 gactgcagac aatgaatcat caaacaccat aatcggtgtt ttttgaagca gcatcctggc
      181 tattgccacc cgttgttttt gacctcccga taatgttact cctctttctc caacaattgt
      241 atcatagcct tttgaaaatt cttctataga ttcatcaatc tgcgctattg atgcagcttt
      301 cttaatttca tccaaggaag tatcaggctc gactattgct atattttcct taatcgttcg
      361 agaaaataaa aagggctcct gaagtaccat tccaatattt tttcttaaat gttccctttt
      421 aatatttttt atactcactc cgcctattgt aatgcagccc atatggtcag gcaaatcata
      481 caagcggttt aaaagataca taagagtagt ttttccggag ccggtacctc ctaatatagc
      541 aaatgtgctt cctgccttta ttgtaaagct gacatttttc aaaacctcag tttctccatc
      601 atatttaaat gtaacatttt taaattcaat atcctttaag atatccggag tgacggaatc
      661 cggactgtca tcctcaacag gttcctttaa tatttcacaa attcttttta atgaaacctt
      721 agctttgcta agctctgata aaatcctccc aaaactgcga agcggccatg aaatcattac
      781 ggtataagaa gcaaatgcca gaaattctcc aagtgtaatt ctatcattta tagcaaatat
      841 agtaccgtat acaacaatga ccataaggag taccccggtt accaaatcgc cgattcccca
      901 ataatatcct aactgatatc ccagcttaac ccatagctct gaaaatgtat tatttctttc
      961 cgtaaatttc tctatttcat gtttttctct gccaaaggcc ctaacaacac gaacacctgt
     1021 taaattttcc tgaattgcag taaaaagctt cccttcggct tcatcagcaa ccaagaattt
     1081 tttggtcaat ataccataaa aaatcgttga atatagcaca actacaggta taaataccaa
     1141 tactgccgca gtaaggtgaa aattcattga aaacattaaa atcaacgata ttataaccag
     1201 catgattacc ctgaatattt cgaccaattg agtactgata aaattacgaa ctacttctac
     1261 atctgatgtt gacctttgta ttatatctcc tgtttgattt ttaatatgcc agctgtatgg
     1321 caatttttgt atgtgctcaa acaacttatc ccttatattt tttataattc cttccgttcc
     1381 cttagcaagg gtaatttttc cgatataatc gcttactccc gttataactg ccaagactaa
     1441 tataaaaaaa gtaaggagcc ataaattttc ttttaaaaat tctatcccgc ctatattatt
     1501 tattgtattc ataaccaatt gaatgtagtt caagggataa tcgcctatga tatggtctac
     1561 ggtaaaccta ataacttgag gcactgcaaa acttgcagaa acctttagta tagttgctgc
     1621 agcagatata aagaaaaacc ttttaaatcc ttttgataat attggtataa tcttaagctc
     1681 tgattccttt ttcatattat cctcaaataa aaaaataaat aaccaatcca tcaggattgc
     1741 ttattaattc tatattatta ttcatttagt gtcaatttaa actcatgctt tattttttaa
     1801 taaaactttc aaaattatcg ctgtatttta ttgttttatc gctgaaaatc cacattgctt
     1861 ccgcttcagg caatgaattt ataaattcaa gaccctcttc aaaaggcatg cagaaaacag
     1921 ctgttgataa tgcatcagcc aatcccgaat ccttgcatat tatggaaatg gcagtaaaat
     1981 attttgcagg atataatgtg tcaggattaa ttatatgatt gtacttctcc ccgtcaacta
     2041 tataaaatct ttcataatca ccgcttgtta ccaaagagct gtcgtctaac ttaactattt
     2101 taataactga ttcttcagct tcggtatcgg gattttggat tcctacattc catggttcat
     2161 tcgaggtaac atttttattt atggctcgta cgtttcctcc tacggacaat aatgcggaat
     2221 tcattccact ttcctttgct attttactta cctgctcggt tgcatatccc ttcgctacgg
     2281 caccaacgtc aagactcata aaaggatctc ttaaaaaaac tgttgattgc tcctcatcaa
     2341 taattatatc ttcaatgtta gtgtgctcag atgcaattct taattcttct tccggtggaa
     2401 gagctgcatt cgcagggtct tcaataccga tggttcgata ttcatgccaa attcttaata
     2461 cggcaccaaa agctatattt gttttaccgt ttgttttctc atacatatcc tttgaaaatt
     2521 ttattaaatc aattatcctt ttgtctactt tgacaggctt tattcctgca ttatcgttga
     2581 tagtcttaat attgtttatg ccttcatagt cattatatat atcataaagc tgatgatatt
     2641 cctttaaatt gtcataaatt atctgtgcgt actcagtgaa ctcttctttg ctctttgaat
     2701 atcccacaat tcttgtaaca gtatcaaaca gcattataaa ttcggattga tagcgaatag
     2761 gttctttatc actgcatccg gttaaactta ttattattaa tattaacaat attacggaaa
     2821 tgattttctt tgccaactta cacctctaat aaaaacaagg ggcagcatct gccccttaga
     2881 taatgattcg ctttgattgt tatactttgt taaatactat tgttataagc cttctcaaaa
     2941 atggttttta agtctacaat tccaactgta acagatgata ctaaatcttc ttcggtagca
     3001 tggccgcttt catcaactgc tataccttta atttcttcaa cagtcttgcc taccacataa
     3061 tctgcaaatg catctgcttg ttcataccat tctttgccta ttgctgaagc ttttttcatt
     3121 ccgtaatctt cgcctaactc aaccttagtt aaaggtgcaa ctgctaggtc tgtagttatt
     3181 ttacctgtat tatcaaattt tacgttagct tgagatgcat ctattattga gcttgttact
     3241 tttccgtctg catcaaatgt tgctacagta taagttgagt aagcttgagc taatccttct
     3301 gcatcagctg ttgcatcttt tgattttgca attgtagtta taactcccaa tcctaattta
     3361 tcagaagcat cagcacctaa attctcagca tttgctacag ctttttcaat agcttcttta
     3421 taaggattaa ttttgattgt tacggatgat actaagtcag cttctgttgt aactccagtt
     3481 tcatcaactg caatagcttt aacttcttca acagtcttgc cttttacata ttcagccaaa
     3541 gcattagctt gttcatacca ttctttttct atgcttgaag ctttacccat tccgtattct
     3601 tctttaagct cttgtttaga cttaaacaca gtatttaaat caggtataat tttaccttct
     3661 ttgctgaatt caattttagt ttgtgcagta tcgattatag tttgcactat ttttccgtct
     3721 ttgtcaactg taactgcaac aattactgaa tctgtttgag ctaatccgtc tttttcgcct
     3781 gcatcctttg atttctcaac agatgatata actgccaaac cagttttaac tgctccttca
     3841 gctaatggtt cttcttcttt aggctcttca gtctttggtt cttcagtttt tggttcttca
     3901 ttttttggtg tttcacccgg ttgatcctgc gtacatgcta cgaaagcaaa tagagacatc
     3961 gctaagatca acactattgc taataacttt ttcattactt tcctccataa attcttaatc
     4021 tatatatata atcagcattt agctgcttat aactttgtga ttaaatcggt acaatattaa
     4081 caaattgtat tacattagtc aacacttttt tgaaaatttt ttaactaatg ttcatcatta
     4141 acaaactgcg ttctgacata agcttcttta aaaggattgg tttcccaaca atcctaaatt
     4201 tatttcatat atccgattct gtgtgatatt tcattcgcag cttctttaat aataacaatt
     4261 aattccttta ttctttcctc attcatcctg aaagcaggac ctgttgtgct taaactgaat
     4321 cttactttcc catcatgatc aaatatcggt gcagctacac attttaatcc ttcaacatat
     4381 tcttcattgt caattgcgta accgtttttc ttcatttctt ctaatttctt aatgaactca
     4441 cctttattcg ttatagtatt attagtatac tgaggaattc ttttcggtaa ataattttta
     4501 attttatctt tattttgata acatataatt attttaccgt ctgcagttgt ataggccggc
     4561 agccttgctc ctattttggt tgaagtttta attgatttgg tgcattctct tttttctatc
     4621 caaacaacat ccagtccgtc aagcattgct acatgaactg tttcttcaat tttttcgcaa
     4681 actgcatcaa taacaggtac cgctgcattt cgtatattca ttgaattaat tactaagtct
     4741 ccaaattgga caaaccgtat acctaatttg tatttttgag tttcttcatc ttgagaaata
     4801 aacccatgat actttaaagt agatataatt ccatgaacag tactcttgtt taaacctaat
     4861 ctttcagaaa tttccgacag cttaagttct tttttttctt cgctgaaaca atctaatatt
     4921 gcaactgccc tgtcaatgga ttgaactctc gttatatctt tcataaacga tactccaaaa
     4981 ttatagcatg tgattttaaa aaaatcaata taaataattt attaacaaac gaattgtgca
     5041 gtatccaatt atttattaat ttttttaata ttatgctata agtattttaa tttcaatgtt
     5101 taaatactac aatacaattt ataatgatac tatatcacta tgtaaattat ttgtcaatct
     5161 gtatgaaaat ttgtcaaata aaaaattttt tgttatagac atgttatgtt aaataagtta
     5221 aaattatgat attaaattat aaggaaggga tataatgaat attactaaag aaaaagcatg
     5281 ggaattgctt aaaaagtaca acaaggatga atttcatctg cagcatgcag atactgtaag
     5341 ctttgtaatg gaatattttg cgaaaaaact gggttttgaa gatgaagcag aattttgggg
     5401 aattgtagga attttgcatg acattgactt tgaaatgtat cctgatgaac attgcgtaaa
     5461 aatgcaggag atactgtccc aaaacggagt agacgaaaaa attattaaat cagctgcaag
     5521 ccatggatac ggacttacag atgttacaat aaaacctgaa catactatgg aaaaaatact
     5581 ttttgcaacc gatgaactga caggattgat aggtgcttct gcattgatgc gtccttcaaa
     5641 gagtacattg gatatggagc ttaagtcatt aaagaaaaaa ttcaaatcac ccggctttgc
     5701 tgcaggctgc tccagagaag caataaaaac cggtgctgaa atgttgggat gggaattaga
     5761 ctatttgttg gatgaaacgt taaaagcaat gcaggaatac gaaaaagcaa gaaattaaaa
     5821 cggatatgcc atgcatatcc tttttaattt acagctagtg ttttttcctt tttcttaaat
     5881 ttgtttttta taaaggtgta tgttaaacaa gaaattacag tgacgggtat aacagtaaaa
     5941 gtattgagaa ttattttatc ggtaacagat aaaaagtttt tttctgcaaa tagtatcata
     6001 atagtcaaaa ccaaaggatg cattaaataa acgtagtaag agctttgtcc gaatacttca
     6061 ataaacccgt aaatattttt taatttatcc ttcattaata gtcctattaa atatacaaaa
     6121 aacaaagaca tagttgaaaa tataaaccaa gcaaaagtag atgccacaga atcataatat
     6181 gaaacaacga tgtaaattat actcatcata atatacccta atataatata taatttattt
     6241 ttattaatcc ataaagaata gttatcgtat tccatagcaa tatatatacc caacatataa
     6301 aacacaatgt atttcaaaaa tatcctgtcc gacaaatcaa atcttatgaa ttcagcacac
     6361 aaatatgtta ttactgcagc aaatataagg attgtcactt tctttccgga atttttcaaa
     6421 ataaaataaa atatgggacc tacaatgtac atttgtataa taattattac aaaataaaag
     6481 tgatatgaca ttgttccctg taaaacactt ttcacaaaaa atttaatatc aatcgcataa
     6541 tatccaaaat atatataagc cgcgtaataa ataatacacc atattaaata tgcaacaatt
     6601 actttggcaa gtcgtttttt gagaaattcc atatacttaa attcacggtt tttattactg
     6661 tagctgtaaa agcttgtcac tccgcttaac aggataaaga cgggtgtagt aaactttaaa
     6721 cttctattta cggtaagcat caaaatatag gggaatgtcc catgaatgta ttcagaaact
     6781 cccgttgctg ttatatggac tatcataact gcaaaacatg caataaatct catataatcc
     6841 aattcataaa gcttgtctct tttttttata atttgctcga ctgcaattgg cggcatatca
     6901 tcactctttc tatattttga ccattttaac atttatacag aagaaaatca attattttag
     6961 aaattcatat atatattcac atatatcata attattcatt attaatgaca aaagatacat
     7021 aatagcgtta ttttataaat ttatttgcaa ttttattaaa aatctacgtt tttgcgtata
     7081 cgtaattgcg aaattgtgct ataattgttt tattaagtat tcattggagg tgacgacatg
     7141 gttgtaagag aagccgtaat aaacagaata aatgaaattt gtgatgacag gggcatagcc
     7201 gtaaataagc ttgcaaactt atcgggaatt acaccatcta ctgtctacag tatatttaac
     7261 agcggcagga aagatatcgg aattgtgctg ataaaaaaga tttgtgatgg atttgatata
     7321 acattggatg aattcttttc ttcagatttg tttaagtcct tagagcagga attattataa
     7381 aggaagatat gaaagataaa gaaagaagag ggacgcttaa aagtgtcccc ccttaatatt
     7441 ccgccttctt atgttaaatt attactcttg aattgtttta aaagatatat atatttttgc
     7501 tctgcagctt caaccttata tattgcatag tccactaaat caggatgtga aacattttca
     7561 aatatgtttt ttgcatcttc ccactctctt tttgcatttt caatattttt aaacatttcc
     7621 tcatctgcgg atttattttt tttaaaactc tctattatac ttttaatact aatctttctc
     7681 tgaacatcat tcatggaaaa aacctcctat ccttattatt atagacagga agttcctttt
     7741 tattcctaat attccaaaaa taatttgcat tatgtataac tataaattat tattttcaag
     7801 ctttaacagg tattctttta tttctatgcc tcccccgtat cctaccatct tgccgttttt
     7861 tcctattact ctatgacagg gaataataat tgaaattgga ttacggttgt ttgccattcc
     7921 tacagccctg cacgattttt catttcctat tgcaattgca atatccttat agcttgcggt
     7981 ctttccgtaa ggaatagttt taagggcatt ccacaccctt tgctgaaatt cggtaccgga
     8041 tgcagatatc ggcaaatcaa aagattttct ttcacctctc agatattctt ctatttgttt
     8101 atgagcttcc tttattaaag gagtttcatt tattttaata tcctcaagct cctcgttttc
     8161 aaaaaaaaca ttcgttatga aattcccatc ctctgcaata cctattctgc cgatatccgt
     8221 atcataataa aatacacttt tcataattcc tcctcttatt atacctctct ttatcctaat
     8281 ttggcacctt tttctactct ttggtcaggc ttaatcaaga ctactccctt ttctgtgtaa
     8341 accccaagca ctagcacttc agatatgaaa tccgcaattt gtctcggagg aaaattcaca
     8401 actgccaaaa cctgtttgcc tataagctca tcaactgtat aacattctgt a
//
LOCUS       DAOSOW010000024         8367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_8450_length_8367_cov_3.424928, whole genome shotgun sequence.
ACCESSION   DAOSOW010000024 DAOSOW010000000
VERSION     DAOSOW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8450_length_8367_cov_3.424928"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..643)
                     /locus_tag="PLD67_01300"
     CDS             complement(44..643)
                     /locus_tag="PLD67_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOT21194.1"
                     /translation="MCGRYYIDIDNEEFKKILEEAQRNIYENFKSGEIFPTNIAPIYI
                     EDDNKIKPLLAKWGFPKWDGKGVIINARAETLNEKLMFKRLSSSNRCIVPASFYYEWK
                     QEEKGKHKYKINKPGSNIYMAGLYNIAANKNKQLSLFDENIDIYYTIITRNSNDSVSH
                     IHSRMPLIFSIEEMNEWLRGQSIDELMKSEHMLTGEITE"
     gene            complement(655..1320)
                     /locus_tag="PLD67_01305"
     CDS             complement(655..1320)
                     /locus_tag="PLD67_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21195.1"
                     /translation="MQIYLKKFIRLIFGLYIYALGLVLCIYANIGLAPWDAFAIGISK
                     VTGISYGNVSILIGITIVIALFFLLKEKIGFGTILNAILIGFFSDLIIDSKIIPYMQN
                     FFTGVLMLVTGQILACIASYLYIGVGLGAGPRDSLMIGLGKKFSNIPIGIVRGSIEAT
                     VLFIGWILGAKVGLGTVIYVFSIGFLLQTTFKLLKFDVKSVVHESVFDTINIFKKIHS
                     ENI"
     gene            complement(1438..2322)
                     /gene="amrB"
                     /locus_tag="PLD67_01310"
     CDS             complement(1438..2322)
                     /gene="amrB"
                     /locus_tag="PLD67_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOT21196.1"
                     /translation="MNKFFKVFTVFLLFITIAGSILAYNTAKNRHEKYFFDGRKNIEC
                     LYYDPSDFINIPEYETSQDIGTIHGCIVPHHLVAKDLIHEVFQKVQKNKYKTVVLIGP
                     DHESTNKGKIFTTLSNWQTPRGVLETDGNLTKELLKYDFVTEDDDKITMEHSTSSIIP
                     FINYYLNDIKIVTLVLTKQVKLEDVEILTDELYKNINLHETLFIASVDFSHYLNLEDA
                     QKKDLISMEAIKNKDINKLMSFTNDNLDSPISVVTLLKMMDKLGAAGDTVLNHSNSEL
                     ILKKKIEETTSYITYLFY"
     gene            complement(2355..7202)
                     /locus_tag="PLD67_01315"
     CDS             complement(2355..7202)
                     /locus_tag="PLD67_01315"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOT21197.1"
                     /translation="MKKLLCLISLMCLILLSCKKTDVLPGLDTSPVEYGVDDKYELIA
                     LKSDKQGIDSTTVFQLTSKEEINNNFIKNNMQIVPEQEYKIEKLSGTTYNIIPAVNLE
                     NDKIYQIVLNDEGYEYSWAFQTKKKFVVESTLPTDRSGYVPSNSGIEIYFSLSGLEEL
                     DDFFEIKPHVDGKFIYKNNTAIFVPDKLEENINYTVTIKKGYGLKDSEQKLEEDYSFS
                     FNTKKEKYAEIYFDRPLTNIYENNAKIIEAYINNENLEFDINIYQYSNADYFAENIND
                     FAETGKFFTGSVDDIEGLSLLNTIKQKPNISYKNIYNTPKGLFEIPGLPKGYYLLEFS
                     TNSGEKNYHFLQINDMLIYYALFENQIVVIASNASDSKGIVDAGVVLNGEHLGFTDND
                     GILIADRDTSKYENKTISLRVEAKGFSDFVYADSFYFFNYYYRSQYEAYKYIRYIDTD
                     RPVYLPTDKINVWGFARHRDDKSVNKVRIELVEANTGLVLDEKTVDLTNIGTYQTEFE
                     IKNITSDILQINVYDNDLKISTKYVSIGEYTKPLYTVKGEFDKKFVYKGESVNYKVKA
                     SFFDGYPVPNLELKFGTFSSNYSGSLEYGGMDATVKTDEKGETVISINTNLTSDSWRP
                     VSIRASLWNAKAEDKPVSLIDGFEVFPHHEMVEIVQNDVEHPENISILLHELDITNYN
                     SQYYGGYEKLRGNPLSGTVQVSVAESYYKKVKKGEYYDFINKVNVVDYIYNYVENVVF
                     NEITEITDGYGNLQIPDFNPERTYKISAEYMSENGPVREWAHVGYYSRYYEKLYYSLE
                     KDKDKDNYRLNDNVSLKLTYNHEDVQNTDYDKLILLIMKNGLIDYKITDNTNLSFEFE
                     DEYIPNVMLNSIYCKNGYMYPVEFTGSIFYDTTEKQIHFDITTDKEEYRPGEEVVLSI
                     KTKDENNKPITADVNISVVDEAYFALFEKRVDTLNSLYARVSSGLVATYLSNIDLSMD
                     NGSGAERGGGGEDYTNFRDEFKDTNVFKTITTDKNGNGELKFKLADNLTSWRITYQGI
                     SDKLFAGSGTKNITASLPFFVDVIMSKEYLKDDKISASLRVFGAEAKESEEVNYEIKI
                     INKETGKELKYKETGIIGDYTNISFDKLDEGSYEIYIYASYKDFEDGIMEEFNVLDSF
                     VYFNNTTYYKLTEDTVLDSVYSNPVITLYNESRSDFYKSLENILANSGKRIDQTVCSM
                     LAAKYINDYFKTDLYFNEDELINEVNKYEIDTWGGYSLFTYSAADVEITAKLINLIDN
                     EYIGYKSKTYFKNILQNPDYNEEIPAALWGLSTYKEPVLLTIYDLLENNTLEIRDKIY
                     LSLALAELGDLNTAKKYYKEFSNSFKESGNYLYFESGADENSSSENYELTALLSLLGC
                     KLKDYETSDKLFKYIYNNPSKFTLSSFEQLIYIMNRDIMKLDEIKDLFGEVTVNSEGK
                     DKTYKLKLFDRESFSVPKDKIKDVKFKNIKGSIACMVEALGNKDDLEKNKTDDFSIKI
                     DYSLKDSTQNQMSYNHSDLVKVTIMPSFSKNVITGNYEITYVVPAGFRFIELDRKNPF
                     WVEENGQKLRFFFHYDRQNFAAIPITFYIQAAQKGEYTVDYVVIKENLEPKLNYLDKT
                     ILSVK"
     gene            7360..8124
                     /locus_tag="PLD67_01320"
     CDS             7360..8124
                     /locus_tag="PLD67_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOT21198.1"
                     /translation="MEYTVKKLSLLAGVSARTLRYYDEIGILKPARINSSGYRIYGKE
                     EVDRLQQILFYRELDIGLDNIKKILSSPDYDDIVALREHKRALLAKREQLNILIDNLD
                     KTIKYREGRIVMNDSEKFKGFKKQLIEENEKKFGQEIREKYGEEAVKKSNEQFMNMSE
                     EQYKEFEELGNSIFEYLKKAMETNDPAGELAQKTAELHKKWITFCWGSYDKEAHAALA
                     QMYVDDERFKENYEKVQKGAAEFLRDAIFIYTGSEK"
BASE COUNT         2650 a         1459 c         1200 g         3058 t
ORIGIN      
        1 tcatatcatt tgagaaatga aagcttagat atagtgaagt caattattcg gtaatttctc
       61 cggttagcat atgctcagat ttcatcaatt catctatact ttggcccctc agccattcat
      121 tcatttcttc aatactaaaa atgagtggca ttctgctgtg tatgtgggaa accgaatcat
      181 tggaatttcg tgttataatg gtatagtaaa tatcaatgtt ttcatcaaac aagctcaatt
      241 gcttgttttt atttgcagct atattgtata atccggccat ataaatattg gagccgggtt
      301 tatttatttt atatttatgc ttgccttttt cttcctgctt ccattcataa taaaaagaag
      361 caggcacaat acacctgttg gatgaagaca gtcttttaaa cattagcttt tcgtttagtg
      421 tttcagctct tgcattaatt attacaccct tgccgtccca ctttggaaat ccccatttag
      481 ctaagagagg ctttatttta ttatcatcct ctatataaat aggggcaatg tttgtaggaa
      541 aaatttcacc gcttttaaaa ttctcataaa tatttctttg tgcctcttct aatattttct
      601 tgaattcttc attatcaata tcaatgtaat atcttccgca catattatcc tcatttaaat
      661 attttcagag tgaatttttt tgaatatatt tattgtatcg aatacacttt catgaacaac
      721 cgatttaacg tcaaatttaa gcaatttaaa tgtagtttga agtaaaaagc ctatgctgaa
      781 aacataaata accgttccaa gcccaacctt agctcccaat atccagccta taaacaaaac
      841 agttgcttca atggagcctc ttactatacc tattggaata ttagaaaatt ttttccctaa
      901 accaatcatt aaagaatccc tgggtcctgc tccaagaccc acaccaatat ataaatatga
      961 agctatgcat gctaatattt ggcctgttac aagcattaat acacctgtga aaaaattttg
     1021 catatatggt attatttttg aatcaataat taaatcagaa aagaatccta tcaatatggc
     1081 gtttaaaatt gtcccgaacc cgattttttc ttttaataga aaaaataatg ctattacaat
     1141 tgtaatacct ataagaatcg atacattacc gtaagatatt cccgttactt tactgatgcc
     1201 tattgcaaag gcatcccagg gagcaagtcc aatattggca taaatacata aaactaaacc
     1261 caaagcatat atataaagac caaatattaa tctgataaat ttttttaagt atatctgcat
     1321 agcatcctcc tgatttcaat aaactaattt tatatgaatt tatattaaat aacaacaaat
     1381 ttttaaatat tgtaaataaa aggtatacaa tatgtgtgtg gatgatgtca aagtatctta
     1441 ataaaacaaa tatgttatat agcttgttgt ttcttcgatt ttctttttta atatgagctc
     1501 tgaattgctg tgatttagta cagtatcacc agctgctccc aatttgtcca tcatttttag
     1561 caaggtgaca acagatatcg gtgagtctaa attatcattg gtaaaagaca tgagtttgtt
     1621 tatgtctttg ttttttatgg cttccatact tatcaaatct tttttttgag catcttccaa
     1681 atttaaataa tgggaaaaat caactgaggc aataaacaat gtttcgtgta agtttatatt
     1741 tttatataat tcatccgtca atatttcaac atcctctaat ttcacctgct ttgtcaaaac
     1801 taaggtaact atcttaatat cattcagata atagttaata aagggaataa tacttgaggt
     1861 ggaatgttcc attgttatct tatcgtcatc ttctgttaca aagtcgtatt ttaataattc
     1921 ttttgttaaa ttaccgtctg tttctaagac acctcttggt gtttgccaat tgcttaatgt
     1981 cgtaaatatt tttcctttat tggtactttc gtggtcggga ccaatcaata ccactgtttt
     2041 atatttattt ttttgcacct tttggaatac ttcatgaatt aaatcttttg caaccaaatg
     2101 atgaggtact atacatccat gtatagtacc gatatcttga cttgtttcat actcaggaat
     2161 attaataaaa tctgatggat cgtaatataa acattcaata ttttttcttc cgtcaaaaaa
     2221 gtatttttca tgcctgtttt tcgctgtatt ataagcaagt atacttccgg caatcgtaat
     2281 aaacaataaa aatacagtaa atactttaaa aaatttattc attatgcacc tgccacttag
     2341 atttatccgg aattttattt cactgataaa attgtcttat caagataatt caatttaggc
     2401 tccaaatttt cttttataac cacataatcc acagtatact ctcccttttg tgcagcttga
     2461 atataaaatg taatagggat tgcagcaaag ttttgcctgt cataatggaa gaaaaatctt
     2521 aatttttgac cgttttcttc aacccaaaac ggattcttcc tatcaagctc aataaacctg
     2581 aaaccggcag gaaccacata cgttatttca taatttcctg ttattacatt tttgctaaag
     2641 gatggcatta tggttacttt aactaagtcg gaatgattgt atgacatttg gttttgtgtg
     2701 gaatctttaa gactatagtc tatttttata gagaaatcat cggttttatt tttttctaaa
     2761 tcatccttat ttccaagtgc ttcaaccatg catgctatac tcccttttat attcttaaat
     2821 ttgacatcct taattttatc cttcgggacg gaaaaacttt ccctgtcaaa taatttcaat
     2881 ttatatgttt tatctttacc ttctgaatta acagtaactt ctccgaacaa atctttaatt
     2941 tcatctaatt tcattatatc tctgttcata atatatatta gctgctcaaa gcttgaaagg
     3001 gtaaacttag aaggattatt ataaatatat ttaaacaatt tgtcgcttgt ttcataatct
     3061 ttaagcttgc atcccaatag cgacaatagt gctgttaatt cataattttc ggaactacta
     3121 ttttcatctg caccactttc aaaatataaa taatttccgg attctttaaa gctgtttgaa
     3181 aattctttat aatatttttt agccgtattt aagtctccca attccgccaa agctaaggaa
     3241 agataaattt tatctcttat ttccaatgta ttgttttcca ataaatcata aattgttaat
     3301 aataccggct ccttataggt gcttaagccc caaagagctg ccggaatttc ttcgttgtaa
     3361 tccggatttt gcaatatatt tttaaagtaa gttttcgact tataccctat atattcatta
     3421 tctatcaagt ttataagttt tgcggttatt tcaacatcag ctgcggaata tgtgaaaagg
     3481 ctgtatccac cccatgtatc gatttcatat ttgttgactt cattgattag ttcgtcctca
     3541 ttaaaatata aatcggtttt aaaataatca tttatatatt tggctgccaa cattgaacat
     3601 acagtttggt caatcctttt tccgctgtta gctaaaatat tttccaggct cttgtaaaag
     3661 tcggagcggc tttcgttata caaggtaatt accgggttag aatatacgct gtccaacaca
     3721 gtatcttccg tcaatttata ataagttgta ttgttaaaat atacgaatga atcaagaaca
     3781 ttgaattctt ccattatccc gtcttcaaaa tccttataag aagcataaat atatatttca
     3841 taagagcctt catctaattt atcaaatgaa atattcgtat agtctcctat aatacctgtt
     3901 tccttatatt tcaactcttt accggtttct ttatttataa ttttaatttc ataatttact
     3961 tcttctgatt cttttgcttc agcgccgaat acccttaggg aggcagatat tttatcatcc
     4021 ttcagatatt ctttgctcat gattacatct acgaagaacg gcaaacttgc ggtaatattt
     4081 tttgtgccgc ttcctgcaaa taatttgtct gaaatccctt gataggttat tctccaggat
     4141 gtcaaattat ccgctaattt aaattttaat tctccattgc cgtttttatc tgttgttatg
     4201 gttttaaaca catttgtatc cttgaattca tctctgaaat tagtataatc ttctcctcct
     4261 ccgccccttt cggcaccact gccattatcc atgcttaaat caatatttga cagataagta
     4321 gcaacaagtc ctgagctaac cctggcatat aaagaattca aagtatccac tcttttttcg
     4381 aacaatgcaa aatatgcctc atctacaacg gatatattta catctgcagt aattggttta
     4441 ttattttcat cctttgtttt tatacttaat acaacttctt caccgggtct gtattcttcc
     4501 ttatccgttg ttatatcaaa gtgtatttgc ttttcagtag tatcatagaa tatagagcct
     4561 gtgaattcaa ccggatacat atacccgttt ttgcaatata tgctgtttag cattacattc
     4621 ggtatatatt catcttcaaa ttcaaagctg agatttgtat tgtcagtgat tttataatct
     4681 ataagcccgt ttttcataat taacagtata agtttatcat aatccgtgtt ttgaacatct
     4741 tcatgattgt atgtgagctt taagcttaca ttgtcattca gtctgtaatt atctttatcc
     4801 ttatcctttt ctaaggagta atacaatttt tcataataac gtgaatagta tcctacatgt
     4861 gcccactctc ttacaggacc attttcactc atatattctg ctgaaatttt atatgttctt
     4921 tcaggattaa aatcaggaat ttgtaaattg ccgtatccgt ctgttatttc cgtaatttca
     4981 ttaaaaacaa cattttcgac atagttatat atataatcaa caacatttac cttatttata
     5041 aaatcgtaat actcaccttt ttttaccttt ttataataac tttccgctac gcttacttgt
     5101 accgtaccgc ttaagggatt acccctcagc ttttcatatc cgccgtaata ttggctgtta
     5161 taattggtaa tgtctaattc atgcaataat atacttatat tttcaggatg ctccacgtca
     5221 ttttgcacaa tttcaaccat ttcatggtga gggaatactt caaatccatc gataaggctc
     5281 acaggtttat cctctgcctt tgcattccaa agacttgctc gtatagaaac gggtctccag
     5341 ctgtcgctcg tcaaattcgt atttatactt attactgttt ctcccttttc atcagttttt
     5401 acggttgcat ccattccgcc gtattctaaa cttccgctat agttactgga aaatgtgccg
     5461 aatttaagct ctaagttcgg taccggatag ccgtcaaaga aagaggcctt tactttatag
     5521 tttacacttt cacccttgta aacaaatttt ttatcaaatt ccccctttac cgtataaaga
     5581 ggttttgtat attcaccgat ggatacatat tttgtggata tttttaaatc attatcgtac
     5641 acatttattt gaagaatatc agaagtaata ttttttattt caaattctgt ttggtaagtt
     5701 cctatattcg ttaagtcgac tgtcttttca tctaacacca agcccgtatt agcttcaacc
     5761 aattcaattc ttaccttatt aacagactta tcatccctat gccttgcaaa tccccataca
     5821 ttgattttat ccgtaggcag gtagacaggc ctgtccgtat caatatacct tatatatttg
     5881 taagcttcat actgacttct gtaataataa ttgaaaaaat aaaagctgtc tgcatataca
     5941 aaatcgctga aacccttagc ctcaactctt aaagaaattg ttttgttttc atatttagaa
     6001 gtatctctat ccgctataag tattccgtca ttgtcggtaa aacctaaatg ctctccgttc
     6061 aataccacac ctgcatccac aatacccttg ctgtcagatg cattcgatgc aatgacaaca
     6121 atttgatttt caaataaagc ataataaatt agcatatcgt taatttgcag gaagtgataa
     6181 tttttttcac ccgaattcgt tgaaaattcc aataaataat atccctttgg aaggccggga
     6241 atttcgaata atccttttgg agtattataa atattcttat aagaaatgtt tggcttttgc
     6301 tttatagtat ttaacaggga taatccttca atatcatcaa cactgccggt aaaaaatttg
     6361 cctgtctctg caaaatcatt gatattttcg gcaaaatagt ctgcatttga atactgataa
     6421 atatttatgt caaattccag attttcatta tttatatatg cttcaattat ttttgcattg
     6481 ttttcgtaaa tatttgtcaa tggcctgtca aaataaattt cagcatactt ttcttttttt
     6541 gtattaaagc tgaaggaata atcttcttct aatttctgct cactgtcctt gagaccataa
     6601 ccttttttta ttgttaccgt ataattgatg ttttcttcta atttatcggg aacgaaaata
     6661 gcagtattat ttttgtagat aaatttacca tcaacatgag gcttaatttc aaaaaaatca
     6721 tccaattctt ctaaaccgct taagctgaaa tatatttcaa tacctgaatt tgaaggtaca
     6781 taaccgcttc tatcggtagg gagggtactt tccactacaa attttttctt agtttgaaat
     6841 gcccaggagt actcatatcc ttcatcattg agtacgattt gatatatctt gtcgttttca
     6901 agatttacag caggaataat attatatgtt gtacctgaaa gtttttcaat tttatactct
     6961 tgctcgggaa ctatttgcat attatttttt atgaaattat tgtttatttc ttcctttgaa
     7021 gtcagctgaa aaacagttgt actgtcaatt ccctgtttat ctgatttaag ggctataagc
     7081 tcgtatttat cgtcaacacc gtattcaacc ggcgaagtat ctaatccggg caacacatca
     7141 gtcttcttgc aagacaacaa aataaggcac attaatgaga ttaagcataa taattttttc
     7201 atttcttccc tcccaaagat tatatgtcaa ttatatcact tatcatatgg tgttacaaga
     7261 cagttcaaga aatccggaat aaataatctt tttatagaat taatatattg acattgacgt
     7321 tacgtaaagg tatacaatat atttgtaagg agtgaaatta tggagtatac agttaagaaa
     7381 cttagtttgc tggcaggtgt gagcgccagg accttaaggt attacgatga gataggaatt
     7441 cttaagccgg caagaattaa ctcatccgga tatcgcatct acggaaaaga ggaagtagac
     7501 aggctgcagc aaatattgtt ttacagggaa ttagatattg gtttagataa tataaagaaa
     7561 atactttctt cacctgatta tgatgatatt gttgctctta gggaacataa aagagccctc
     7621 cttgcaaaga gagaacaatt aaatatttta attgataatc tggataaaac gattaagtat
     7681 agagaaggga gaattgttat gaatgatagc gaaaaattta aaggattcaa gaaacaatta
     7741 attgaagaaa atgaaaagaa attcgggcag gaaataagag aaaaatatgg tgaagaagcc
     7801 gtgaaaaaat ccaatgaaca gtttatgaac atgtctgagg agcaatataa ggaatttgaa
     7861 gaattaggca attcaatttt tgaatatctt aaaaaggcaa tggaaacaaa tgaccctgca
     7921 ggagaacttg cacaaaaaac agctgaactt cataagaagt ggattacctt ttgctggggc
     7981 tcttacgaca aagaagcaca tgctgctctt gctcagatgt atgtggatga cgaaagattt
     8041 aaagaaaatt atgaaaaggt acaaaaagga gctgcagaat ttttgcgtga tgcaatattt
     8101 atttatacgg gaagcgaaaa ataatattgt tataccttgt actttcgcta tttattgaca
     8161 aagcttttac tttgcattat tattgaaagc aaaagcttat caagagatta ggtgaaaata
     8221 tgagtttgat acctatagac gcacacccgt catggaataa ttttttgacg gatagtataa
     8281 tattggaact ggaaactata gaaagaaaaa taggcagcaa tataaatcct gctcctgaaa
     8341 atgttctgcg tttcatgaaa tgtaatc
//
LOCUS       DAOSOW010000025         8248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_8635_length_8248_cov_3.856219, whole genome shotgun sequence.
ACCESSION   DAOSOW010000025 DAOSOW010000000
VERSION     DAOSOW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8635_length_8248_cov_3.856219"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PLD67_01325"
     CDS             complement(<1..705)
                     /locus_tag="PLD67_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOT21199.1"
                     /translation="MAKIKESDYLYSASRVKSIEKHMLTRERAEKMIDAKTMEDAIRV
                     LDDCYYGYLNEITDFDNYESLLADEHKKTYDFIKSIAPDAEHFNIFLYPYDYHNLKVL
                     MKNEYLNKDETDLLVDTGTIDLNVLKYALKERNFSDLTENMGKALNEIIEDFPKTKDP
                     QLIDIILDRYCFDEMLKAAEKTGNKFIIDYVKMQIDSINIKTYVRLKKMNKSWDFFSK
                     VFLHGGTIHEHIFIKNY"
     gene            complement(733..1314)
                     /locus_tag="PLD67_01330"
     CDS             complement(733..1314)
                     /locus_tag="PLD67_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HOT21200.1"
                     /translation="MSIDNITSKILSDAQNNADSTLMNAENTKQEIITKAKSEAETII
                     KTEAERAEKDAVDLKNRRVSAAELQGRKMLLSAKQEAIKKSFDKAIEKLKAMPEDEYL
                     NFIAQEILKIPNCEGSIVLNAKDRENIGERLVKTVNEKLGAEKVVLSKNTVKASGGFV
                     LKRGSVEINSTFETLLDSMKDELTGEIANALFK"
     gene            complement(1352..1828)
                     /locus_tag="PLD67_01335"
     CDS             complement(1352..1828)
                     /locus_tag="PLD67_01335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOT21201.1"
                     /translation="MELTMGGFLAILGAAVASMAGIGSAWGVAIAGMAAGGVIAEDPK
                     KFGPTLMLQALPGTQGIYGLLIAFIIMNKIGVLGGNIAALTTPQGLMFLAAALPAGIT
                     GTVSAIWQGKVAATGVHLIGKRPEEIAKAMVYAAMVETYAVLALLASFLMVNGIQI"
     gene            complement(1829..3811)
                     /locus_tag="PLD67_01340"
     CDS             complement(1829..3811)
                     /locus_tag="PLD67_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HOT21202.1"
                     /translation="MTIVNMNKVNIIGLDSIKTELMKRIMDLGVVEISSQDTKLTDPE
                     WTSYVKKDGNENVVNNLDVKISQINEVLNTLELYDTSKRPLFTTRKTISSDEFRKKLE
                     ENNSVSENVAKVLELNKSLSELCSEGNKIEASILSLKPWSSYDIPLDMTETKYSSIIM
                     GTIPNIVEIDELKSQLLQTTDRCIINVIGSDKDQYYCSFICLTEEKEDILEFLKQYGF
                     SAVIFKDLSGTAAENIIQYENSLKEISAKRETIDKRITELVSCKEEIQLFHDHLIIER
                     DKNKILSNMLKTDTTFYIEGWIPEVSKDKVQKVLDEYECWYDFKKPEEGEEFPILLKN
                     NAFSGPFDSITELYSLPSPSNIDPTTVMAPFYVLFFGMMLGDVGYGFLFSLGCFIILK
                     KFDIEGTFGKMLKVFFWGGISTMFWGVMYGSWFGDGLVVGARHLFNINLPLTYVWVDP
                     LKEPMTVLLVSFIMGLVHLFVGMGMQAYMLIRDGKIKDAITDIGFWYLFIIGPILWLA
                     GDMIIPGSPLPVIGKWMTLFAVIGLVLTQGRAKDNIISKLISGVLSLYGITGYLGDVL
                     SYSRLLALGLATGVISSVLSLIGSMVGGIPGKIMFVLVFVFGHTLNLAINGLGSFVHS
                     ARLQYVEFFGKFYEGGGDAFNPFTKKTKYIKIKEEI"
     gene            complement(3798..4124)
                     /locus_tag="PLD67_01345"
     CDS             complement(3798..4124)
                     /locus_tag="PLD67_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21203.1"
                     /translation="MSIEVIKVIKEAEEKAEAIKKDAAAQARLVITNANAQAQDILDE
                     ARKTAESHRSEVLSKAESEGQLLYDGIINDAAKECDKILTKANDNMDAAASIILERIV
                     KTSDNS"
     gene            complement(4444..4869)
                     /gene="fabZ"
                     /locus_tag="PLD67_01350"
     CDS             complement(4444..4869)
                     /gene="fabZ"
                     /locus_tag="PLD67_01350"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOT21204.1"
                     /translation="MVYNINDIQNVIPHRYPMLLVDKIIELQEGQKAVGIKNLTMNEQ
                     FFQGHFPGNPVMPGVLIIEALAQTGAFAILSLEKFKNKTVYFGGIQSAKFKRKVLPGD
                     TLKLVTEITKMKSTFGIGSAVAYVDDQVAAEATLIFAVE"
     gene            complement(4874..6109)
                     /gene="fabF"
                     /locus_tag="PLD67_01355"
     CDS             complement(4874..6109)
                     /gene="fabF"
                     /locus_tag="PLD67_01355"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOT21205.1"
                     /translation="MKRRVVITGMGVVSPIGSGDIFWENVKKGVCGIDFIESFDTSDF
                     SVKIAAEVKDFDPTLYMEKKEAKRMDRYSQFAIAAADIAVKDSGLNTKEVDSDRFGCI
                     VGSAVGGIHTIEEEHAKLLEKGPGKISTFFIPMMLSNMASGLVAVKYQAKAVNVSTVT
                     ACATGANAIGESYRRIQDGTCDVMIAGGAEAAITPCSIAGFTALTTLSTTRDINRASI
                     PFDKDRHGFVMGEGAGVMVLEDYEHAVKRGAKIYGEIVGYGATCDAYHMTSPDPSSEG
                     ASKSMLNAILDAGIEPKDITYINAHGTSTLYNDKFETQAIKNVFKEESENIYVSSTKS
                     MTGHLLGAAGAVEAIICVKAIEDSFIPATINHKETDPELGLNFVPNVGISKDFEYALS
                     NAFGFGGHNATLVVKKFKG"
     gene            complement(6287..7018)
                     /gene="fabG"
                     /locus_tag="PLD67_01360"
     CDS             complement(6287..7018)
                     /gene="fabG"
                     /locus_tag="PLD67_01360"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004444747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOT21206.1"
                     /translation="MKTAIITGASKGIGAVIAKRLNELNYNLVLNYRSNTALIEELID
                     NFTNKDTNNIIVKCDISLYEDAKKLIDEAYNNFGTVDVLINNAGITKDNILPLMTEEE
                     FDEVIDINLKGTFNCCKHIARRMIKQKYGRIINISSVVGLSGNAGQVNYAASKAGVIG
                     MTKSMARELGKKNILVNAVAPGFIKTEMTDKIPEDIKAVMLKNIPLQRLGQPEDIADC
                     VEFLISDKASYITGQVISVNGGFYM"
     gene            complement(7039..7974)
                     /gene="fabD"
                     /locus_tag="PLD67_01365"
     CDS             complement(7039..7974)
                     /gene="fabD"
                     /locus_tag="PLD67_01365"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010298219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOT21207.1"
                     /translation="MNKTAFVFPGQGAQYAGMGKDFYDNFKESREIFHRANEALGFDI
                     TKLCFEGPNEELSITKITQPALLTVCLAIYEVLKKRADDSVVMGGLSLGEYSALTAAG
                     AMDFETAVQLVYKRGNYMQNTVPLGEGGMLALLGCTDEEAIAFCKDITLKYGLLEPAN
                     FNYPGQIVAGGKSVAIDNAIDKAPRFNIKKAVKLQVSAPFHTSMLKPAGDKLKEDLAK
                     IIFKKPVTNVISNVDSVYYEKSKIAEKLEKQVYNPVRWEACVRKMIADGVNTFVEIGP
                     GKTLSGFIKRIDKNVKSINIENVQGLNTYLQFAGA"
     gene            complement(7967..>8248)
                     /locus_tag="PLD67_01370"
     CDS             complement(7967..>8248)
                     /locus_tag="PLD67_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003366936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOT21208.1"
                     /translation="TGHPVRVLKNRLVTKFKELEKTNATVEEMESLGRGKLYMAAVEG
                     DVDYGSVMSGQIAGMVNKEQSCKEIIDEMFEEAEKIMKNLVFIAGGGNE"
BASE COUNT         2377 a         1766 c         1159 g         2946 t
ORIGIN      
        1 atagttttta ataaatatat gttcatgtat ggtaccgcca tgcaaaaata ctttactgaa
       61 aaaatcccat gacttattca ttttcttaag ccttacatat gtcttaatgt taattgaatc
      121 aatctgcatc tttacataat caataataaa tttatttcct gtcttttctg cggctttaag
      181 catttcatca aaacaatacc tgtctagaat aatatctatg agctgagggt cttttgtctt
      241 tggaaaatct tcaattattt catttaatgc ttttcccata ttttcggtta aatcagaaaa
      301 atttctttct tttaaagcat attttaaaac gtttaagtca atggttccag tatcaacaag
      361 aagatctgtt tcatccttgt tcaaatactc gtttttcatt aatactttca aattatgata
      421 gtcgtatggg tacaaaaata tattgaaatg ttcagcatca ggagcaatgg acttaataaa
      481 gtcatatgtt ttcttatgct catccgcaag gaggctttcg taattatcaa aatccgttat
      541 ttcatttaag taaccataat aacaatcatc caatactcta atggcatctt ccattgtttt
      601 tgcatctatc attttttctg ctctttcacg ggtaagcata tgcttttcta tacttttcac
      661 tcttgaagca gaatataaat aatcagattc cttaatcttc gccaaagtca ttcctccttt
      721 ccgacaggag tcttatttaa aaagcgcatt tgctatttca cctgtaagtt cgtccttcat
      781 ggaatctaaa agggtttcaa aagtactgtt aatttcaacg ctgccccttt taagcacgaa
      841 tccgccgctt gccttaaccg tatttttgct taatacaact ttttctgctc ccagcttttc
      901 gttgacagtc tttactaatc tttcaccgat attttccctg tctttggcat ttaaaactat
      961 acttccttca caattaggaa tttttaaaat ctcctgtgct ataaaattca aatattcatc
     1021 ttccggcatt gctttaagct tctcaattgc tttatcaaaa ctttttttaa tagcttcctg
     1081 ttttgctgat aatagcattt ttctgccttg aagttcagca gcagataccc ttctgttttt
     1141 taaatcaact gcgtcttttt ctgctctttc cgcttcagtc tttattattg tttcagcttc
     1201 acttttagct ttggttatta tttcctgctt tgtattttca gcattcatca aagtagaatc
     1261 agcgttattt tgtgcatcgc ttaaaatctt tgatgttata ttatcaatgc tcatatcatc
     1321 acatccttat aataaatttt tacacactcc tttatatttg aattccattt accataagga
     1381 atgatgcaag aagtgctaaa actgcatatg tttcaaccat agctgcgtat accattgctt
     1441 ttgcgatttc ttcaggtctt tttccaatca agtgaacacc tgtagctgca accttacctt
     1501 gccaaatagc tgatactgtt ccggttatac cggcaggaag tgctgctgcc aaaaacatta
     1561 aaccctgagg tgttgtcaaa gcagctatgt tgcctccaag tacaccgatt ttattcatta
     1621 taataaatgc aataagaagt ccatatatac cttgtgttcc gggcagagcc tgaagcatta
     1681 atgtgggacc gaatttctta ggatcctccg caataacacc accagcagcc attcctgcta
     1741 tagcaactcc ccatgcacta cctattcctg ccatagatgc tacagcggca cctaaaatag
     1801 ctaaaaaacc acccattgtt aattccattt aaatttcctc cttaatttta atatatttag
     1861 tttttttagt gaaaggatta aatgcatctc ctccaccttc ataaaattta ccgaaaaatt
     1921 ctacatattg aagccttgcc gagtgaacga atgaaccaag accgtttatg gcaagattaa
     1981 gtgtatggcc gaatacaaaa accaatacaa acattatttt cccgggtatg ccgccgacca
     2041 tggagcctat taaacttaga acagatgaaa taacacctgt tgcaagtccg agtgcaagaa
     2101 gcctcgaata tgaaagaaca tcacctaagt aacctgttat tccatatagc gacaatacac
     2161 cgcttattaa cttgctgatt atattatctt ttgctcttcc ttgagtcagg acaagaccta
     2221 ttacagcaaa taaagtcatc catttaccta taacaggtaa tggcgaacct ggaattatca
     2281 tatctcctgc taaccacaaa ataggtccta ttataaataa ataccaaaaa cctatatcgg
     2341 ttattgcatc cttaatttta ccatctctta ttaacatata agcctgcatt cccattccta
     2401 caaataagtg aacgagaccc atgatgaatg ataccaataa gacagtcata ggctctttta
     2461 aggggtctac ccacacatat gtcaaaggca agttaatatt gaaaagatgt cttgctccta
     2521 caactaaccc atctccaaac cagcttccat acattacacc ccaaaacata gtggatattc
     2581 cgccccaaaa aaatacctta agcatttttc caaaagtacc ttctatatca aattttttga
     2641 gtataatgaa acatcccaat gaaaaaagaa aaccatagcc tacgtcccca agcatcatgc
     2701 caaagaataa aacgtaaaac ggtgccataa ctgtagtcgg gtctatattc gatggtgaag
     2761 gcagactata cagctctgtt attgaatcaa atggtcctga aaaagcatta ttcttcaata
     2821 atatcgggaa ttcttcccct tcctccggct ttttaaaatc ataccagcac tcatattcgt
     2881 ctaaaacttt ttgaactttg tccttgctta cttcaggtat ccaaccttca atataaaagg
     2941 ttgtatctgt ctttagcata ttacttaata ttttattttt atctctttcg attatcagat
     3001 ggtcatggaa aagttggatt tcttctttgc aggaaactaa ttccgttatt cttttatcga
     3061 ttgtttctct cttagcagat atttctttta agctgttctc atattggata atattttccg
     3121 ctgcagtacc gcttaaatcc ttgaatatca cagcactaaa accatattgt tttagaaatt
     3181 ccagaatatc ctccttttcc tcggtaaggc aaataaatga gcaataatac tggtccttgt
     3241 cgctgcctat tacatttata atacatctgt cagttgtttg aagaagctgg gattttaact
     3301 catctatttc aacgatatta ggtattgtac ccattataat gcttgaatat ttagtctccg
     3361 tcatatctag aggaatatca taactgctcc atggttttaa actcaaaatg gatgcttcta
     3421 ttttatttcc ctcagagcat agctcactta atgacttatt taattcaaga accttggcta
     3481 cattttcaga tacgctgttg ttttcctcta atttttttct aaattcatcc gatgatatcg
     3541 tttttcttgt ggtaaataaa ggtcttttac ttgtgtcata caattcaagg gtgttcagta
     3601 cttcgttaat ctgagaaatt ttaacatcga gattgttaac tacattttca ttcccatctt
     3661 tttttacata agaagtccat tcggggtctg taagttttgt atcttgtgag cttatttcca
     3721 caacgcctaa atccataatt ctcttcataa gctctgtttt tatgctgtct aaacctatta
     3781 tgttgacttt attcatatta actattgtca ctggtcttca cgatcctttc cagtataatt
     3841 gaagctgcgg cgtccatatt atcatttgcc tttgttaaaa ttttgtcaca ttcctttgca
     3901 gcatcattaa taatgccgtc ataaagaagc tgtccctcgg attctgcttt gcttaaaact
     3961 tcagatctgt gagactctgc tgtttttctt gcttcatcca agatatcttg agcttgtgca
     4021 ttagcattgg taataactaa tcttgcttgt gctgcggcat cttttttaat tgcttcagcc
     4081 ttttcctccg cctcctttat taccttaatt acttcaattg acactatacc accaccttta
     4141 tgatatttct agaaattttc tatcattcca cattaactta tattaaagta aaaacaagca
     4201 tttgtcaagt ttttgtctaa atccaaaaat actaaaagaa gagtattttt ggattttagt
     4261 gtttaaagta actttgatta aaccataaat tatgattctt ctttacttaa taagtatttg
     4321 tttacaatag taaaatttat cggtaaatgt caaaaaaaat aacttatatc acaagagtga
     4381 taaaagttat tcttatattc attaacaaac ataggcatac cctttactaa agaaaggcca
     4441 gggctattca accgcaaata tgagcgttgc ttctgccgca acttggtcat caacataagc
     4501 aactgcagaa ccgattccaa aagttgattt catctttgtt atttctgtaa caagctttaa
     4561 tgtatctccc ggaagaacct tccttttaaa ctttgcgctt tgaattcccc cgaaataaac
     4621 cgtcttattt ttgaattttt ctaaggacaa tattgcaaaa gctcctgtct gagctaaagc
     4681 ttcaataatt aaaacgccgg gcattaccgg gtttccggga aaatgacctt gaaaaaactg
     4741 ctcattcatt gttaaatttt taattcccac agccttttga ccttcttgaa gctcgattat
     4801 tttatcgact aaaagcatgg gatatctgtg aggaattaca ttttgtatat catttatatt
     4861 ataaaccata atttcatcct ttaaattttt ttactactaa ggttgcatta tgaccgccaa
     4921 aaccaaatgc atttgacaag gcatactcaa agtcctttga aattcccaca ttaggcacaa
     4981 aatttaaacc taattccgga tctgtctctt tatgatttat ggttgcagga ataaaggaat
     5041 cttctatagc tttaacgcag atgattgctt caactgcccc cgctgctcca agcaaatgtc
     5101 ctgtcattga ttttgtagag cttacatata tattttctga ttcttcctta aaaacattct
     5161 taatagcctg ggtttcaaat ttatcattgt ataaggtact tgttccgtgg gcatttatat
     5221 atgttatatc ctttggctcg atacctgcat ccaaaatagc atttaacatt gacttagatg
     5281 cgccttctga gcttggatca gggctggtca tgtgatatgc atcacaggtt gcaccatacc
     5341 ccactatttc accatatatt tttgctcccc ttttgacagc atgctcataa tcttcaagca
     5401 ccataacacc ggcaccttct cccattacaa atccatgtct atccttatcg aatggaatag
     5461 aagctctgtt tatgtctctt gttgttgata atgtagtaag agccgtaaat cctgcaatag
     5521 agcatggagt tattgctgct tcagcgcctc ctgcaatcat aacatcgcag gttccatcct
     5581 gtattcttct gtatgattct cctattgcgt tagctccggt ggcacaagcc gtaaccgtac
     5641 ttacattgac agccttcgcc tgatacttaa cggcaactaa tccggatgcc atatttgaaa
     5701 gcatcattgg aatgaaaaaa gttgaaatct ttcccggtcc cttttccaac agctttgcat
     5761 gttcttcttc tattgtatgt attccaccga ctgcacttcc gacaatacag ccaaacctgt
     5821 cggaatcaac ctccttagta ttaagacccg aatccttgac tgcaatatct gcagctgcta
     5881 tggcaaactg gctgtatctg tccatccttt tggcttcctt tttttccata tacaaagtag
     5941 ggtcaaaatc cttcacttca gctgctattt ttacgctgaa atccgatgta tcaaaagatt
     6001 ctataaaatc aattccgcat actccctttt ttacattttc ccaaaaaata tctcccgagc
     6061 caatagggct tactaccccc attcctgtta taacaactct tcttttcatt attctctcct
     6121 taattattac cgttatgtca tcctgagcgt agcgaaggat ctaatgagat tcttcactgc
     6181 gttcagaatg acacttcgtg tattcatagc aatgacatga aaattgggga cattccttct
     6241 gtctacacag acttgcccta tgagaaagtg tgtccccata ccttttctac atataaaatc
     6301 cgccgttaac agatataacc tgccctgtaa tataggacgc tttgtctgat attaaaaatt
     6361 caacacaatc cgctatatct tccggttgcc ctaatctttg aagaggaata tttttcaaca
     6421 tcactgcctt aatatcctca ggtattttat ctgtcatttc agttttaata aatcccggtg
     6481 ccacagcatt taccaaaata tttttcttgc ctaattctct tgccatggac tttgtcatac
     6541 ctattactcc tgcttttgaa gctgcatagt ttacctgtcc tgcattgccc gaaagaccaa
     6601 ctaccgatga tatgtttata attctgccgt acttttgctt tatcattctt cttgcaatat
     6661 gtttgcaaca gttaaaggta ccctttaaat ttatatcgat aacttcatca aattcttctt
     6721 ctgtcattaa gggaagtata ttatcctttg taattccggc attattaata agcacatcaa
     6781 ccgtgccaaa attattgtaa gcttcatcta taagtttttt tgcatcttca taaagagata
     6841 tatcgcactt aactattata ttattagtat ctttattagt aaaattatct ataagttctt
     6901 ctattaaagc agtgttgctt ctgtagttca acactaaatt gtaattcaac tcattcaatc
     6961 ttttggcaat tacggctcct atccctttgg aagctcctgt tattatggca gtcttcatgg
     7021 ttttccttcc tttgtatttt aagctcctgc aaattgcaga tatgtattta atccttgaac
     7081 attttcaatg tttattgatt taacattttt gtcaattctt ttaataaatc cggaaagtgt
     7141 cttaccgggt cctatttcta caaatgtatt tactccgtct gcaatcattt ttctgacgca
     7201 cgcctcccac ctgacaggat tatacacttg tttttcaagc ttttctgcaa ttttactttt
     7261 ttcgtaatat acgctatcca catttgatat aacattagtg acaggttttt tgaatattat
     7321 tttagccaaa tcttctttta atttatcacc tgcgggttta agcattgatg tgtgaaatgg
     7381 agcgctcact tgaagcttta cagctttttt gatattaaac cgaggtgctt tatcaatagc
     7441 attatcaatg gcaacacttt ttccacctgc tactatttgt cccggatagt taaaattagc
     7501 aggctccaat aaaccatatt ttaaagttat atccttgcaa aaagcaattg cttcctcatc
     7561 cgtacagcct aaaagagcta acatgcctcc ttctcctaag ggaacagtat tttgcatgta
     7621 atttcctctt ttatatacta actgtacggc tgtttcaaag tccattgcac ctgcagcagt
     7681 taaggcagaa tattcaccaa ggcttaatcc gcccattaca acagaatcat cagccctctt
     7741 ctttaatact tcataaatcg caagacatac tgtcaaaagt gccggctgag ttatttttgt
     7801 aatgctgagt tcttcattag gtccttcaaa gcaaagctta gttatatcga atcctaatgc
     7861 ttcatttgct ctatgaaata tttctctgct ttctttaaaa ttatcataaa aatcttttcc
     7921 catgccggca tattgagcac cctggcccgg gaaaacaaaa gctgttttat tcattgccgc
     7981 ctcccgcgat aaatactaaa ttcttcatga ttttttctgc ttcttcaaac atttcatcga
     8041 taatttcctt gcaactttgc tctttattta ccatgcctgc aatttgacct gacattacgg
     8101 aaccgtaatc tacatcacct tcaacagcag ccatataaag tttgcccctt cccaatgact
     8161 ccatttcttc aacagttgca ttggtttttt ccaattcttt aaatttagtc actaatctgt
     8221 tttttaatac tctcacagga tgacctgt
//
LOCUS       DAOSOW010000026         8131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_8813_length_8131_cov_3.919019, whole genome shotgun sequence.
ACCESSION   DAOSOW010000026 DAOSOW010000000
VERSION     DAOSOW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8131
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8813_length_8131_cov_3.919019"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..299)
                     /locus_tag="PLD67_01375"
     CDS             complement(102..299)
                     /locus_tag="PLD67_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21209.1"
                     /translation="MNYFKHKKKCSPANKPSFFKFTEILGLMIATIGAIIIVQMLPVK
                     VWLFVLGILLIILGCTLFRLL"
     gene            complement(401..1783)
                     /gene="asnS"
                     /locus_tag="PLD67_01380"
     CDS             complement(401..1783)
                     /gene="asnS"
                     /locus_tag="PLD67_01380"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOT21210.1"
                     /translation="MKTTDIKDIFLSPENYKEVRIEGWIRTIRDSKNFGFIELNDGTF
                     LKNVQVVFENNLSNFEEIKKFSTGSAITVRGNIVLTPEAKQPFEIKATEIALEAPSDS
                     SYPLQKKRHSMEYLRTIAHLRPRTNTFSAVFRVRSLAAYAIHKFFTERGFIYAHPPII
                     TASDAEGAGEMFRVTTLDLDNIEKVDGKTDYSKDFFGRSANLTVSGQLEAEIFALAFK
                     KTYTFGPTFRAENSNTARHAAEFWMIEPEIAFADINDNMDLAEDMVKYVVSYVMENAK
                     EELEFFNNFVEKGLLEKLDNVVNSEFGRISYTEAIDILMKNNDNFEYKVKWGTDIQTE
                     HERYLSEKIFKKPVFAVNYPKEIKAFYMRMNEDNKTVAAMDLLVPGIGELIGGSQREE
                     RLEFLEKRMDEMGVPKDNMWWYLELRKYGGVKHSGFGLGFERLIMYLTGMSNIRDVIP
                     FPRTPKNADF"
     gene            complement(1795..2430)
                     /locus_tag="PLD67_01385"
     CDS             complement(1795..2430)
                     /locus_tag="PLD67_01385"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOT21211.1"
                     /translation="MKTALIVGNGDETAKRIIENIKYDYLICADGGLEKVSNYGIIPD
                     IIIGDFDSVASNVLKQYENRIPIEKFPSEKDFTDMELAVELAVSKGYKNIILTGASGS
                     RLDHTLGNILLMEKYFKDGVNISIIDNNNEMKIISDNSELFIEYKEGYFISIIPITEF
                     IQGLCLEGFKYNLDNVDVERGSTLCISNQIRDSKGKITLRKGRAIVIISKD"
     gene            complement(2427..3083)
                     /gene="rpe"
                     /locus_tag="PLD67_01390"
     CDS             complement(2427..3083)
                     /gene="rpe"
                     /locus_tag="PLD67_01390"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOT21212.1"
                     /translation="MKNKLAPSILSADFSNLGEAVKLVEEGGADYIHIDVMDGHYVPN
                     ITIGPAVVKDLRKTTSLIFDVHLMIENPDNFIEHFYKAGADIITVHQEATVHLHRTIQ
                     KIKSYGLKAGVSLNPATPISVLNEIIKDVDMVLLMSVNPGFGGQSLIENVKYKFYDLK
                     QLLNKYNLDIDIEIDGGVNYSNLKEILSWGANVIVAGSAIYHAEDIVLETKKFKEIMS
                     "
     gene            complement(3093..3971)
                     /gene="rsgA"
                     /locus_tag="PLD67_01395"
     CDS             complement(3093..3971)
                     /gene="rsgA"
                     /locus_tag="PLD67_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOT21213.1"
                     /translation="MIEGIITKGVGGNYYVDIGSKIIECRARGLFRLKNIKPLVGDKV
                     LIRITEEDEDMGYIEEIKERTSEIKRPPVANAQQLVIIFSVKNPEPSFILLDKLLIAA
                     KSNNLNPVICINKSDLANEEEKENILNIFKNTGYKIIFTSKYDEDSLQNLKDILKDRL
                     NVFSGPSGVGKSSLMNAIEPGFYLETGEISDKLKRGRHTTRHAEIFKLGFGGYAVDTP
                     GFSSFELEGVDEYSLKSYYPEIVKYDDGCKFLDCLHYKEPGCMIKEAVNSDLISRVRY
                     NNYIKLLEQIKESKPY"
     gene            complement(3984..5945)
                     /gene="pknB"
                     /locus_tag="PLD67_01400"
     CDS             complement(3984..5945)
                     /gene="pknB"
                     /locus_tag="PLD67_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HOT21214.1"
                     /translation="MIGRTLGNRYEIIEKIGGGGMSNVYKAKCNVLNRFVAIKILRDE
                     LTQDSDFVNNFKQESLSAASLAHPNIVNIYDTGFEDDIYYIVMEYVKGETLKNYIKRK
                     GSLSEKEAVKISRQVAEALKHAHSHNIVHRDIKPHNILLTEDGTAKVTDFGIARASTS
                     STINNTSNVIGSVHYFSPEQARGGYVDEKSDIYSLGIVMYEMVTGTLPFDADNHISVA
                     MKQIQEKPVPPSKRVKNVKISKNYEDIIMKCLEKHQSFRFQNTDELLKKLDALNGNNN
                     PVKDETEIIDSPTIEIPALNDLEENVINIDLIDENSDKAFKSFFSGNEEDENVEKSIN
                     NKKITIAAILSALVIAVIGGVFLFKSFLYVPEVPVPSLLGRSEEEAKKLIEDLGLVFK
                     VSKREYSSEYDEGYVMEQSIDEGSKVKENYPIEVVISRGEKEIVVPNLIGKYAIEAGG
                     ILNNAGLSEGETTEENSDTYLAGQIIDQYPLPNTPARENDKVSYVVSIGPKIIYVKMP
                     NVLNLNLETAKLSIVQHGLAVGQVNEEPSEEIEKGLVMRQSITADQEVEQGTSIWLTV
                     SSGKPEEPDEPTPEEGVFPLAISLPADRDSVSIVIQRIASDGREVVYSEEVDTSEGNI
                     LVNVKGKGTGIFEIYIDNELYDRVEITFE"
     gene            complement(5935..6687)
                     /locus_tag="PLD67_01405"
     CDS             complement(5935..6687)
                     /locus_tag="PLD67_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOT21215.1"
                     /translation="MDVFFDTNKGLMRENNEDSLIVEQFDNYCLYAVADGMGGHKAGE
                     VASSIAIEEIRNFFVECMKDENFQPPSFIMESVKLSNDKIREVSLNNEDCCGMGTTVT
                     MAVIDKLNNIIYIGNIGDSRAYLLRNNEIKQITTDHTYVNELIKDGRITEEEAKTHPK
                     RNVITRALGSEEYVHADIFELELLKNDILLLCTDGLTTHVSDDNMLEIIKEYGTSESV
                     QKLIKQANDNGGTDNITIIIVDNIIRGETYDR"
     gene            complement(6696..7724)
                     /gene="rlmN"
                     /locus_tag="PLD67_01410"
     CDS             complement(6696..7724)
                     /gene="rlmN"
                     /locus_tag="PLD67_01410"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003365995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOT21216.1"
                     /translation="MKKIDDLSFEELKDAVLKIGEPKFKANQIFDWIHNKTVESFDDM
                     TNLSQKTKAALKNEYEFTKAIIELKLESKLDETKKYVIKLDDSNIIESVYLKYKFGNT
                     ACISSQVGCKMGCHFCASTKNGFIRNLTAAEMLKQIYLIQNDINEKISNVVIMGSGEP
                     LENFDNIVRFLNIINDERGQNISMRKITLSTSGIVPNIYKLADENIPITLAISLHAST
                     QEKREQIMPIARKYKLPEILKACDYYVKTTGRRLTFEYIMIKGFNDSREDAENLSRLL
                     KNKLANVNLIPCNYVKEANIEPSEDLDIMKFKKILTDNGINATVRRELGSDINAACGQ
                     LRNDLLKM"
     gene            complement(7775..>8131)
                     /locus_tag="PLD67_01415"
     CDS             complement(7775..>8131)
                     /locus_tag="PLD67_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOT21217.1"
                     /translation="DYVIADVPCSGLGIIRRKPEIKYNETDLKDITEIQYKILENASK
                     YLKQSGELVYSTCTTNKEENIEIINRFLKNKNFILADFSDIINEDFKTAKDGYIEIYP
                     HLHGMDGFFIAKLKRL"
BASE COUNT         2287 a         1685 c         1104 g         3055 t
ORIGIN      
        1 aaatagtggc caacgccatt atttttttgt gtccccacca aattggggac attcgtcatt
       61 ccctcggaga ctgtccatct attagaggtg tgtccccata cctataacag cctgaataat
      121 gtgcaaccca aaattatcaa taatattcct aacacaaaca accatacttt aacaggaagc
      181 atttgaacta taataattgc tcctatggtt gcaatcataa ggcctaatat ttcggtgaat
      241 ttaaaaaatg atggtttatt tgcaggactg catttctttt tatgcttaaa gtaattcata
      301 atatccccct tagaatttat gtagagctct actattatta cattctattc aagcataaaa
      361 aaaagataca aagaaatttt ctttgtatct tacctatacc ttaaaaatct gcatttttag
      421 gggttcttgg gaacggaatt acatccctta tgttgctcat gcctgttaaa tacataataa
      481 gtctttcaaa accaagtccg aaacctgagt gtttcacacc gccgtacttc cttaattcca
      541 agtaccacca catattatct tttggaactc ccatttcatc cattcgcttt tcaagaaatt
      601 caagtctttc ttctctttgg ctgccgccta tcaactcacc tattccggga accaataagt
      661 ccattgctgc cactgtttta ttatcttcat tcatccgcat ataaaatgcc tttatttcct
      721 taggataatt aactgcaaat acaggctttt taaatatttt ttccgacaaa tatctttcat
      781 gctcagtctg tatatctgtt ccccatttaa ccttatattc gaaattatca ttgtttttca
      841 tcaatatatc tattgcttct gtgtatgata ttcttccaaa ctctgaattt accacattat
      901 caagtttttc taaaagccct ttttccacaa agttattgaa aaactccagt tcttctttgg
      961 cattttccat gacatagctt acgacatact taaccatatc ttctgccaaa tccatattat
     1021 catttatgtc ggcaaaagca atttcaggct ctatcatcca aaattctgcg gcatgccttg
     1081 cagtatttga attttcggct ctgaatgtag gtccaaaagt ataagttttc ttaaatgcca
     1141 aagcaaaaat ttcagcttct aattgcccgc ttaccgtaag atttgcagac cttccgaaaa
     1201 agtcctttga atagtccgtt tttccgtcaa ccttttcaat attatccaaa tccagtgtgg
     1261 taactctgaa catttcacct gcaccttctg catctgaagc agtaattatc ggcggatgcg
     1321 cataaataaa tcccctttcg gtaaaaaatt tatgtatggc ataagcagct aagctcctta
     1381 cacgaaaaac tgcggaaaaa gtgttggtgc ggggtcttaa atgcgcaata gtccttaaat
     1441 attccattga atgtcttttc ttttgcaaag gatagcttga atctgacggt gcttccaagg
     1501 ctatttctgt tgctttaatt tcaaaaggct gctttgcttc gggagttaaa actatgtttc
     1561 ctcttaccgt aatggcactg cctgttgaaa attttttaat ttcttcaaaa ttactcaagt
     1621 tgttttcaaa aacaacctgc acatttttaa gaaatgttcc gtcatttaat tctatgaatc
     1681 caaaattttt agaatctctt attgttctta tccagccctc tatacggact tctttgtaat
     1741 tttccggcga taagaatata tcttttatat ccgttgtttt catgaaccct cctgttaatc
     1801 tttagatata attactatag ctcttccttt tcttaatgta atttttccct tactgtctct
     1861 tatttgattg ctgatacaaa gagtcgagcc tctttcaaca tcaacattgt caagattata
     1921 tttgaatcct tccaaacata gtccctgaat aaattctgta atggggatta tcgatataaa
     1981 ataaccttct ttgtattcta taaacagctc cgaattatcg gaaataattt tcatttcatt
     2041 attgttatca attattgaaa tattaactcc atctttgaag tatttctcca taagcaaaat
     2101 gttgcccaag gtatggtcta atcttgaccc ggaagctccc gtgagtatta tatttttgta
     2161 tcctttggaa acagccagtt caacagctaa ttccatgtct gtaaaatcct tttcggaagg
     2221 aaatttttca ataggtattc tgttttcata ttgtttcaga acattactag ctaccgaatc
     2281 aaaatcaccg attattatat ctggaattat tccgtaattt gaaacttttt caagccctcc
     2341 atccgcacat atcaaataat catacttaat attttcaatt attcttttgg cggtttcatc
     2401 tccgtttccg acaatcagcg ctgttttcat gacatgattt ccttaaattt ctttgtttcc
     2461 aaaacaatat cctctgcatg gtaaattgca gagcctgcaa ctataacatt tgcaccccag
     2521 cttaaaattt cctttaaatt tgaataattc acgcctccgt caatttcaat atcaatatct
     2581 aaattatatt tatttaataa ttgctttaaa tcataaaatt tatacttgac attttctatc
     2641 agactttgtc ctccaaaacc cgggtttacg ctcatcagca aaaccatatc cacatcttta
     2701 ataatttcat ttaaaacaga tatgggtgtt gccggattta atgatactcc tgcttttaag
     2761 ccgtatgatt ttattttctg gatagttctg tgaagatgta cggttgcttc ctgatgcacg
     2821 gtaattatat ctgctcctgc tttataaaag tgctcgataa aattatccgg attctcaatc
     2881 attagatgca catcaaatat gagagatgtt gtttttctta aatcttttac aactgccggc
     2941 cctattgtaa tgttaggaac gtaatgacca tccattacat ctatatgtat gtaatctgct
     3001 cccccttctt ccacaagttt tacggcttct cctagattag aaaagtcggc ggacaaaatt
     3061 gaaggggcta atttattttt catacttcct ccttaataag gcttactttc ttttatttgc
     3121 tccaaaagct ttatatagtt attatacctt actctgctga ttaaatcact attaacggct
     3181 tcttttatca tacaaccggg ctctttgtaa tgaaggcagt ccaaaaattt acagccatca
     3241 tcatatttta ctatttctgg ataataactt tttagagaat actcatcaac tccttccaat
     3301 tcaaaggaac tgaaacccgg cgtatcaacc gcatacccgc caaagcctaa tttaaatatt
     3361 tccgcatgcc tggtcgtatg ccttcctctt tttaatttat cgctgatttc acctgtttcc
     3421 aaataaaatc cgggctctat ggcattcatt aaggatgatt ttccaactcc tgaaggtccg
     3481 gaaaacacat ttaatctgtc cttaaggata tcttttaaat tctgtaggga atcctcatca
     3541 tacttgcttg taaaaataat tttgtagcct gtgtttttaa atatgttaag tatattttct
     3601 ttttcttctt catttgctaa atctgattta ttaatacaaa taacagggtt taaattattt
     3661 gactttgctg caatcaaaag tttatccaac aatataaagc ttggttccgg gttttttacg
     3721 gagaaaatta ttacaagctg ctgagcattg gcaacgggag gtcttttaat ttcgcttgtt
     3781 ctctctttaa tttcttcgat ataacccata tcctcatctt cctctgtaat cctgataagg
     3841 actttatccc ctaccaaagg tttgatattt ttgagtctga acaagcctct tgctcggcat
     3901 tctattattt tgctgccgat atcaacataa taatttcctc cgacaccctt tgttatgatt
     3961 ccctcaatca ttaaaactcc tttttattca aatgttattt caactctgtc atataattca
     4021 ttgtcaatat atatttcgaa tattcctgtt ccttttccct taacattaac taggatattt
     4081 ccttccgaag tatccacttc ctctgagtac acaacttcac gtccgtcact ggctattctt
     4141 tgaatcacta ttgatacact atccctgtcc gcaggaagag atattgccaa ggggaatact
     4201 ccttcttccg gtgtaggctc gtcaggttct tccggcttgc ccgaacttac cgtaagccat
     4261 attgatgttc cttgttcgac ttcctgatcg gctgttatac tttgtctcat aaccaagccc
     4321 ttttctattt cttcgctggg ctcttcattt acctgcccaa ccgcaagacc atgttgaaca
     4381 atcgaaagct tcgccgtttc taaattcaaa tttaacacat ttggcatttt cacatatata
     4441 atcttcggac ctatactcac cacatagctt actttatcat tttccctagc cggtgtatta
     4501 ggtaaaggat attgatcaat tatctgtcct gccaaatatg tgtcagaatt ttcttctgtc
     4561 gtttctcctt ctgaaagccc tgcattattg agaattcctc ccgcctctat agcatacttg
     4621 cctattaaat taggaacaac tatttctttt tctcccctgc ttattaccac ttctatgggg
     4681 taattttctt taaccttaga gccctcatca atactttgct ccattacata accttcatcg
     4741 tattcagaag aatattctct ttttgaaact ttgaatacca gacccaaatc ttcaatgagc
     4801 ttcttggctt cctcttcact tcttcccaat aaactcggca ccggaacttc cggcacatac
     4861 aaaaatgact taaatagaaa tactccgcca attactgcta taaccaatgc gcttaaaatt
     4921 gccgctatag taattttttt attgtttatg ctcttttcaa cattttcatc ttcttcattt
     4981 ccgctaaaga agcttttaaa tgctttgtcg gaattttcgt caattaaatc tatattaata
     5041 acattttctt ctaaatcatt aagcgcagga atttcaatag tcggtgaatc tattatttcc
     5101 gtttcgtctt ttacgggatt attatttccg ttcaatgcat caagcttttt cagcagttca
     5161 tcagtgtttt ggaatctgaa gctttgatgt ttttcaagac acttcattat aatatcttca
     5221 taattttttg atattttaac atttttcacc ctttttgaag gcggaacggg tttttcctga
     5281 atttgtttca tagcaaccga aatatggtta tctgcatcaa aaggaagagt acctgtcacc
     5341 atttcataca ttacaattcc caatgagtaa atatctgatt tttcatcaac atatcctcct
     5401 cttgcctgtt caggagaaaa gtaatgcact gagccgataa catttgaagt gttgtttatg
     5461 gtagaacttg ttgacgctct tgcaattccg aaatcagtaa cctttgcggt tccgtcctca
     5521 gtaagaagta tgttgtgggg cttaatgtcc ctgtgtacta tattgtgtga gtgagcatgc
     5581 tttaaagctt ctgccacctg tcttgaaatt ttcacggctt ccttctcact cagactgcct
     5641 tttcttttta tatagttttt taaagtttcc ccttttacat attccataac tatataatag
     5701 atatcgtctt caaatcctgt atcgtaaata tttacaatat taggatgagc gagacttgcc
     5761 gccgataaag attcttgttt gaagttatta acaaaatcag aatcctgagt taattcatcc
     5821 cttaaaattt ttatagctac aaagcggttc aaaacgttgc acttagcctt atatacattt
     5881 gacattcctc cgccgccgat tttttctatt atttcatatc tgtttcctag tgttctacct
     5941 atcataagtt tcacctctaa ttatattatc aacaataatt atggtaatat tatcagttcc
     6001 gccattatcg tttgcttgtt taatcaattt ttgcacactt tctgatgtgc cgtattcttt
     6061 tatgatttcc aacatattat catcggatac atgagttgta agaccgtcgg tgcaaagaag
     6121 cagtatgtca ttttttaata attccaattc aaaaatatca gcatgtacat attcttcact
     6181 gcctaaagcc cgagttatta cattcctttt aggatgtgtt ttggcttctt cttccgtaat
     6241 tctgccatcc tttatcaact cattcacata agtatggtca gttgtaattt gtttaatttc
     6301 attgttcctt aacaaataag ctctgctgtc gccaatgttt ccaatgtaaa ttatattatt
     6361 aagcttatct attacagcca tggttacggt tgttcccata ccgcaacaat cttcattatt
     6421 taatgacact tctcttattt tgtcattact cagctttacg ctttccataa tgaatgatgg
     6481 gggctgaaaa ttttcatcct tcatacattc aacgaaaaag tttcttatct cttcaatagc
     6541 aatggagctt gccacttcac cggctttgtg ccctcccatt ccgtcagcca cagcatacag
     6601 acaataatta tcaaattgct cgactattaa actatcttca ttattttctc gcattaaacc
     6661 cttatttgta tcaaagaaca catccataca gtacctcaca tttttaacaa atcatttctg
     6721 agctgaccac aggcagcgtt aatgtcactg cccaattctc ttcttaccgt tgcattaatt
     6781 ccgttatccg tcaatatttt tttaaatttc ataatatcca aatcttcgct tggttcaata
     6841 tttgcttctt ttacataatt gcatggaatt aaattaacat ttgctaattt atttttcaat
     6901 aatctgctta agttttccgc atcttcacgg ctgtcattaa aaccctttat cattatatat
     6961 tcaaaggtaa gtcttcgccc tgttgtttta acatagtaat cacatgcttt taatatttcg
     7021 ggaagcttat attttcttgc aatgggcata atttgctctc ttttttcctg tgtggaagca
     7081 tgaagagaaa ttgccaatgt tatcggaata ttttcatcag ccaacttata aatatttggt
     7141 actataccgc ttgtggaaag agtaattttt cgcatggaaa tattttggcc tctttcatca
     7201 tttataatat ttagaaatct cactatatta tcgaagtttt cgagaggctc tcccgagccc
     7261 ataataacaa catttgatat tttttcattt atatcatttt gaatcaggta tatttgctta
     7321 agcatttctg cagcagtcag atttctgata aaaccgttct tagtggaagc acaaaaatga
     7381 caacccatct tgcatcccac ttgtgatgaa atgcaagcgg tattgccgaa tttatatttt
     7441 aaataaacac tttctattat gttgctatca tctaacttta ttacatattt ttttgtttca
     7501 tccaattttg attcaagctt taattcaatt attgcttttg taaattcata ttcgttttta
     7561 agcgctgcct tggttttttg agataaattg gtcatatcat cgaagctttc gacagtctta
     7621 ttatgtatcc agtcgaatat ttgatttgct ttaaatttag gctcgcctat tttaagcact
     7681 gcatctttta attcctcaaa gcttaaatca tcaatttttt tcatattacc cttctttaac
     7741 agacattatg aattgaattt tgttgtcaat tatatcataa ccttttcaat ttcgcaataa
     7801 aaaacccatc cataccgtgc agatgcggat aaatttcaat atagccgtct ttggctgttt
     7861 taaaatcttc atttataata tcagaaaaat cagctaaaat aaagttcttg ttttttaaaa
     7921 atctattaat tatttcaata ttttcctctt tgtttgtggt acaggtagaa taaaccaatt
     7981 cgcctgattg ttttaaatac tttgatgcat tctcaagtat tttatattgg atttctgtta
     8041 tatcttttaa gtctgtttca ttatatttta tttccggttt tcgtcttatt ataccgagtc
     8101 ccgaacacgg aacatcagct ataacataat c
//
LOCUS       DAOSOW010000027         8007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_8994_length_8007_cov_3.924044, whole genome shotgun sequence.
ACCESSION   DAOSOW010000027 DAOSOW010000000
VERSION     DAOSOW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 8007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8007
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8994_length_8007_cov_3.924044"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="PLD67_01420"
     CDS             <1..1385
                     /locus_tag="PLD67_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOT21218.1"
                     /translation="ITGPNTGGKTVSLKTLGLFTLMGMAGLHLPAEYGTEISVFSAVY
                     ADIGDEQSIEQSLSTFSSHMTNIVKILKEVDEKSFVLFDELGAGTDPTEGAALAIAIL
                     DTLHERKILTAATTHYSELKLYALTTEGVINGSVEFDVETLSPTYKLLIGIPGKSNAF
                     EISRKLGLQQEIIDKAKKNIETEKIEFEDALKQIEENRIYIEEKKQEIDRLESESKKK
                     HSDFLMKERKSLEKSEKIIDEANYEARKIVENAKREAAEIIKELRKLNLEMDKDKNRR
                     VNELRQALNEKTKELTENIYSDIIEEEDTIDTDTPLKIGDSVTVRSLNQKGFIISDVD
                     DSQTVMVQIGLIKTKVKKSDLIKIKSDEEVKQKTNASRKVRLRTSSINPTIDIRGYNL
                     DDALLELDKYLDDAFMSNLNEVQVIHGKGTGVLREGVRQFLKNHKHVKESRLGTFNEG
                     GDGVTIVKFK"
     gene            1449..2531
                     /locus_tag="PLD67_01425"
     CDS             1449..2531
                     /locus_tag="PLD67_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21219.1"
                     /translation="MNVRDKYSNKAVIKYTGAFIAWVIGSGFATGQEALQFFSSYGYL
                     SYGVLLVNLIGFLFLGQVLLVTGFEHKTESSFNHFKYFCGEKIGTIYSWLIPITLILI
                     MPVLISGAGATLSEYYGVNRYVGSFLMSVLVLSVYLIGFEHLIKIVSAIGPAIIIFSL
                     LVGAITLANDYGNFSDVPKYQFKLNELQTAPHWTISAILYLSLNFLSGSVYFTELGKS
                     AYNRNDVKYGAFFGALAIILNIAIMNTAILLNAENISTLAIPVLYLAKKISYMLGAIF
                     SIILILGMFSSCSAMMWSICNRFTRGGKRGNRIFAVFISILTFVLGLFSFSELIGTFY
                     PLVGYVGLFFIGSVIYKGIKYNIIKN"
     gene            2542..2763
                     /locus_tag="PLD67_01430"
     CDS             2542..2763
                     /locus_tag="PLD67_01430"
                     /inference="COORDINATES: protein motif:HMM:NF040441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PF20097 family protein"
                     /protein_id="HOT21220.1"
                     /translation="MERIIKDVNCPKCGQVMEEGYIHSRKQILWSEDDKPRLFDFYDE
                     NLVTMPIRRANKVPAWRCKECKIAIFRYE"
     gene            2885..3664
                     /locus_tag="PLD67_01435"
     CDS             2885..3664
                     /locus_tag="PLD67_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOT21221.1"
                     /translation="MRQYVVDAFAEKVFEGNPAAVCILDEWLPDEVMLNIARENNLAD
                     TAFVVKDGTNFKLRWVTPGGEIDLCGHATLATGYVISRFIAPELSEVVFQTKSSGILK
                     VLKKGELYEMDFPAYKLKKVEVTNAMADAIGVKPIEAYMGRDLLCVLESEDEVRNLNP
                     NLEKVKLLDGLLFHVTAKGNEFDCVSRSFVPKLSGTEDPVCGSGHCHIIPYWAERLGK
                     DNLIAYQASQRGGTLYCKYQGDRVIMGGKVALFSTADINIE"
     gene            3735..4814
                     /locus_tag="PLD67_01440"
     CDS             3735..4814
                     /locus_tag="PLD67_01440"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT21222.1"
                     /translation="MEREIVRLDMDRDFEAFEDLLKISFGEATNSKKEMYEWLFENNP
                     YNKSGNMMYLLKEGDKVIGCDGLLPNELYVNGKTLLTAHSVKSMTHPDYKRQGIFRKM
                     TENSCERGKQDGVDVIIGLANDQSYPAYQKFGWPTLFEKEVYVKPILINNILKRRIKI
                     GFLSEAANSVYTVFMKNKLKNLMDKEIKFEILDRVPEEIQKCWDRYKSKYNVLLVRDY
                     KYLNYRYNERPDINYVTILARHKGEIIGFAILHNAVANGSNMTSAVEFFTDPDNERYI
                     KALANGIAKYCYDKGLEYAVIGTGHYGEYRKVLLNNGFMVTRKPPKNNMMIAHILSDK
                     VTLEEITGHEKWHITQGDGETELDL"
     gene            5104..5973
                     /locus_tag="PLD67_01445"
     CDS             5104..5973
                     /locus_tag="PLD67_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOT21223.1"
                     /translation="MIKLIVDSTCDLNCEILDNHDVEVIPLSITIDDKTYLDGVDITT
                     DRVYEYMKDGKVPKTSQVSFESVSQAVEKCISKESDFIYLAFSSKLSGTYSFAKKIID
                     MYKEKYPERKMAIIDSKGGAGGTGLIAIQVLKMIERNLPFEYILNQAQFMVQHIVYRF
                     TISDLEWLSKGGRISKSAGYVGNVLNIKPYLVLDDGLIKVSKMIRGSRKTIQTLIQDI
                     EDGTDKFKNQIIGISHADDLDTALMVEQKIKDSIKGCKTTIFKIGAVLGVHLGIGGVG
                     VFFFDEKPEFYEL"
     gene            6126..7833
                     /gene="argS"
                     /locus_tag="PLD67_01450"
                     /pseudo
     CDS             6126..7833
                     /gene="argS"
                     /locus_tag="PLD67_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361362.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
BASE COUNT         2872 a         1158 c         1638 g         2339 t
ORIGIN      
        1 taataacggg acctaatacc ggaggaaaaa ccgtttcttt aaaaacctta gggcttttca
       61 ctcttatggg aatggcaggg cttcatctcc ctgccgaata cggcaccgaa atatctgttt
      121 tcagcgcagt ctatgccgat ataggcgatg agcagagcat cgagcaaagc ttgagcacat
      181 tttcctctca tatgacaaat atagtgaaaa tattaaaaga ggttgatgaa aagtcattcg
      241 ttcttttcga cgagctcggt gcgggaacag accctacgga aggtgctgca ttggcaattg
      301 caatacttga cactctccat gaaagaaaga ttctgactgc tgcaaccaca cattactcag
      361 agcttaaatt atacgctctt acaaccgaag gagtaataaa cggaagtgtt gaatttgatg
      421 ttgaaacatt aagtccgacc tacaagcttt taatcggtat acccggtaaa tccaatgcct
      481 ttgaaatatc aaggaaacta gggcttcagc aagaaattat cgataaggcc aaaaagaaca
      541 tagaaaccga gaaaattgaa tttgaagatg ccttaaaaca aattgaagaa aaccgaattt
      601 atatcgaaga gaagaaacaa gaaattgaca gactcgaatc agaaagcaaa aaaaagcaca
      661 gcgacttttt gatgaaggaa agaaaatcac ttgaaaaaag cgaaaaaatt atagatgaag
      721 ctaattatga agcaagaaaa atagttgaga atgccaaaag ggaagctgct gaaattatca
      781 aggaattaag aaaattaaac cttgaaatgg ataaggataa aaaccgccgt gtaaacgaat
      841 taagacaggc actaaatgag aaaacaaagg agcttacaga aaatatttat tcggatatta
      901 ttgaggaaga agatacaatt gacacagaca cacctttaaa aatcggtgac tccgtaactg
      961 taagaagtct aaatcaaaag ggattcatta tttctgatgt ggatgattca cagactgtaa
     1021 tggtccaaat aggactaatt aaaacaaagg ttaaaaaatc cgacctaata aaaataaaat
     1081 ccgatgaaga agtaaaacaa aaaaccaacg catcccgtaa ggtcagacta agaacttcgt
     1141 caatcaatcc tacaattgat atcagaggat acaatcttga tgatgctttg ttggagctgg
     1201 ataaatattt ggatgatgcc tttatgtcaa atttaaacga agtccaggta attcacggta
     1261 aaggtaccgg ggtcctgcgt gaaggagtaa ggcaattctt aaaaaatcac aagcatgtta
     1321 aggaatcaag acttggcacc ttcaacgaag gcggcgatgg tgttacaata gtaaaattca
     1381 agtaaatgac aaggggacgg gggtactgtc ataattcaga ggcggtagct gtcttaaggg
     1441 attaaattat gaatgtacgg gataaataca gtaataaagc tgtaataaag tatacgggag
     1501 cttttatagc ttgggttatc ggttcgggat ttgcaacggg acaagaagct ctgcagttct
     1561 tttcaagtta cggatactta agctatgggg ttcttctggt taatttgata ggttttttat
     1621 ttttggggca agtattatta gttacaggat ttgaacataa aaccgaaagt tcatttaatc
     1681 attttaaata cttttgcggc gagaaaatag gaacaattta ttcttggctg attccaatta
     1741 ccttgatttt aattatgcct gtcttaatct ccggtgccgg tgctaccctt tccgagtatt
     1801 atggagtaaa tcgctatgtc ggttcttttc ttatgtccgt cttggtttta agtgtctatt
     1861 taattggatt tgaacattta ataaaaatag tttcagccat aggacctgct attattattt
     1921 tttccttgct tgtgggagcg ataacactag caaatgatta cggcaatttt tctgatgtac
     1981 caaaatatca atttaaactc aacgagttgc aaacagctcc acattggacg ataagtgcta
     2041 ttttgtactt atcgctgaat ttcttaagcg gcagcgtata ttttactgag cttggcaaat
     2101 cagcatataa tcgaaatgac gtaaaatacg gagcattttt tggggctctt gcaataattt
     2161 taaatattgc aattatgaat actgcaatct tattgaatgc tgaaaacata tcaacattag
     2221 caataccggt cttatattta gcaaaaaaaa tatcttatat gttaggagca attttttcaa
     2281 ttatattaat attaggtatg ttttcttctt gttctgcaat gatgtggtca atatgcaacc
     2341 gatttacaag aggcgggaaa agaggcaaca ggatttttgc agtctttatc tcgatattaa
     2401 cctttgtgtt agggttattc tcgttcagtg aacttatagg taccttttat cccctcgtag
     2461 ggtatgttgg attgtttttt attggttctg taatatataa aggaattaag tataatatta
     2521 ttaagaattg aggtgcataa tatggaaaga ataataaaag atgtaaattg tcccaaatgc
     2581 ggacaagtga tggaagaagg atatattcat tcacgaaagc aaatattatg gtccgaagat
     2641 gataagccaa gactgtttga tttctatgat gaaaatttag tcacaatgcc tataagaaga
     2701 gcaaataaag ttcccgcatg gagatgcaag gaatgtaaaa ttgcaatatt ccggtatgaa
     2761 taattatgat ggtataatat ttagtaaatg ctaccattta tgattaatcc atgatacaat
     2821 atttaataaa aaggtaaatt tattattaat attaaataaa aataaaagaa agggagtgta
     2881 aaagatgaga caatatgtag ttgatgcatt tgcagaaaag gtttttgaag gaaatcctgc
     2941 agcggtatgc attttggatg agtggctgcc ggatgaagtt atgcttaata ttgccagaga
     3001 aaacaactta gccgatactg cttttgtggt taaggatggc acaaatttca aattaagatg
     3061 ggttactccc ggcggtgaaa tagatttatg cgggcatgca acccttgcaa ccggatacgt
     3121 tattagcaga tttattgctc ctgaattatc ggaagttgtt tttcaaacca aaagcagcgg
     3181 aatattgaaa gtccttaaaa aaggtgagct ttatgaaatg gattttcccg cttataaatt
     3241 aaaaaaggta gaagttacaa atgcaatggc ggatgctata ggagttaaac ctatagaagc
     3301 atatatgggg agagatttac tctgcgtact ggagtccgag gatgaagttc gaaatttaaa
     3361 tccgaactta gaaaaggtaa aattactgga cggattgctt tttcatgtta cagccaaagg
     3421 aaatgaattt gattgtgtat caaggtcatt tgtacctaag cttagcggta ccgaagaccc
     3481 ggtatgcggt tcggggcatt gccatataat cccttattgg gccgaaagat taggaaaaga
     3541 caatttaatt gcttatcagg catctcaaag aggcggtacc ttgtattgta aatatcaagg
     3601 ggacagggtg ataatgggag gcaaggtagc attgttttcc accgcggata taaacataga
     3661 atagtttttt atttacataa agaaaatatt attaagatga aacacataat atcgatatta
     3721 aatatggagt acttatggaa agagaaatag taagattgga tatggataga gattttgagg
     3781 catttgaaga tttattgaaa atctctttcg gtgaagcaac caactcaaag aaagaaatgt
     3841 atgaatggct ttttgaaaat aacccttaca ataaatccgg aaatatgatg tatttattaa
     3901 aggaagggga taaggttata ggttgtgacg gattgttgcc aaacgaactt tatgttaacg
     3961 ggaaaacttt gttaaccgct cattcggtta aatccatgac ccatcccgat tacaaaagac
     4021 agggtatttt cagaaaaatg acggaaaact cctgtgagag gggaaagcag gacggtgtgg
     4081 atgtgataat aggtttggca aacgaccaat cttatccggc ataccaaaaa ttcggctggc
     4141 ctacactttt tgaaaaagaa gtatatgtaa agcctatact tattaacaat attcttaaac
     4201 gaagaattaa aattggtttt ttgtcagaag cagccaattc tgtctatact gtatttatga
     4261 agaataaact aaaaaatctg atggacaaag aaattaaatt tgaaatactt gacagggttc
     4321 cggaagaaat tcaaaaatgt tgggacagat acaaatcaaa atataatgtt cttttggtca
     4381 gagattataa atacctgaac tacagatata acgaaagacc ggacattaat tatgtaacca
     4441 ttcttgcaag gcataagggg gagattatcg gctttgcaat acttcataat gcagttgcaa
     4501 acggctctaa tatgacatct gctgtagaat tttttactga ccccgataat gaaagataca
     4561 taaaagcatt ggctaatggc atagcgaagt attgctatga caagggactt gaatatgccg
     4621 ttattggaac ggggcattac ggtgaataca ggaaggttct tttgaataat ggatttatgg
     4681 taacccgcaa gcctccaaag aataatatga tgattgctca tatattatct gacaaagtaa
     4741 ccttagagga aataacaggt catgaaaagt ggcatataac tcaaggagac ggcgaaacag
     4801 agctagactt ataactgtat tgtatccgtt tttttaagag gttttcaaaa tagcagtaaa
     4861 atactcctaa tacgcctcta taattctttt aaagcatctt ggtcaggaaa aaccaagctt
     4921 tggtttttcc tgagttcttt tatatttata agcaccactc cggaatttat gtaggtgctt
     4981 ttctcaggca tatcaagcct aagctgcaaa taatgtttgt ttacagttaa aattcatgtt
     5041 aaaatatagt ttgagattat atatataata caattattca tatgtaatgg ggagaaggta
     5101 ataatgatta aattaatagt ggacagcaca tgtgatttga attgtgaaat tttggacaac
     5161 catgatgtag aggttattcc gctaagtatt actattgatg acaaaaccta tttagatgga
     5221 gtcgatataa ctacggacag agtttatgag tatatgaaag acggtaaagt ccccaagact
     5281 tcacaagtgt catttgagtc tgtatcccaa gccgttgaaa agtgtatttc aaaagaatca
     5341 gattttattt atttggcttt ttcatccaaa ttatcaggca catacagttt tgccaagaaa
     5401 ataatagata tgtataagga aaaatatcct gagagaaaaa tggcaataat tgattctaaa
     5461 ggcggagccg gagggaccgg tttaatagct atacaagtac ttaaaatgat tgaaagaaac
     5521 ttaccctttg aatatatctt aaaccaggca caattcatgg ttcaacatat agtttacaga
     5581 tttacaatat ctgatttaga atggctgtca aaaggaggca gaataagcaa gtctgcagga
     5641 tatgtaggaa atgtattaaa tattaaacca tatctcgttt tagatgatgg acttattaaa
     5701 gtatccaaaa tgattcgcgg cagcagaaaa actattcaaa ctttgattca agatattgaa
     5761 gacggtacag ataaatttaa aaatcagatt atcggcataa gtcatgcgga tgatttagat
     5821 actgctttaa tggtggaaca aaaaattaag gattcaatta aaggatgcaa gacaacaatt
     5881 tttaaaatcg gtgccgtttt aggagttcat ttgggaatag gcggtgtagg agtttttttc
     5941 tttgatgaaa aacctgaatt ctatgaatta taatattatt aatagtatat aaaaatagtt
     6001 attgcattag ttgacacatt aaaaatgctg tgatattatt acaaaaaata gaagttacaa
     6061 cctgccgaac atatcaaatg gtaggtttta gttttaataa ttcgtaatta ccttggagga
     6121 gagagatgga ttttaaggaa aaagtggctg aaatttttac atccatagaa accgagctgt
     6181 caatggatga tgctcttcaa ttaatagaga taccacccag tagcgatatg ggagattatg
     6241 cggtaccgtg ttttaaattt gcaaagaagt acaggaaatc tcctgcagca atagcaaatg
     6301 aaattgtaga aaaaatagga gcagttgaag aattcgaaaa aaattgaaag tgcaggacca
     6361 tatgtaaatt tctttgtaaa taaatctcag tttgcaaaaa aaatacttaa acaagctttt
     6421 gaagaaaaag aaaattacgg cagaacgaat gtgggtgaag gcaagactgt tatagttgag
     6481 ttttcatctc cgaatattgc aaaaccgttt cacatagggc atatacgctc aacattaata
     6541 ggaaatgcca taaataatat ttataaatac cttggttata atactgttgc aataaatcat
     6601 ttgggggatt atggaacaca gttcggaatg cttatttcgg catataaaaa atgggcaact
     6661 cctgaaataa ttgaagcaat cgaaaaggac cccatacctg aacttttaaa gctgtatgta
     6721 agatataatc aggaaattga aacacatccg gaataccagg aagaagcaag gtactggttt
     6781 aatcaattag aaaaaggcaa tcaagaagca tatcaattat gggcatggtt tagggaagtt
     6841 agcttaaaag aatttaaccg cgtatatgat atgttcggta ttaagtttga ttcatatgcc
     6901 ggagaaagct tttattcgga taaaatgcct gcaataatcg atgaattgga agataaagga
     6961 ctgcttaaag attcggaggg agcaaaaatc gtagagcttt ccgagtttgg tctgacggat
     7021 gcattaatcc aaaagagcga cggcgctact ctatatgtaa ccagggatgt ggcagctgca
     7081 aaatacagaa aagaaacata taatttttac aaaaatatat acgtggttgg tgcacagcaa
     7141 aaattacatt ttgaccagtg gagaaaaata atagatttaa tgggatatga ctgggcatat
     7201 gactgtgtac acgtaatgtt tggaaccgta agcctggaag gcggtgctat agctacaaga
     7261 aaaggaagag tagtattttt ggaagatgtt ttgaataaag caattgaaaa aactaccgaa
     7321 attattaatg aaagaaatcc gggcttggaa gacagggaga aagttgcaaa gcaggttggt
     7381 ataggtgctg taatattcca agagcttttc aataacagaa taaaagacta tgtattttct
     7441 tgggataaaa cattaagctt tgaaggtgaa acaggacctt atgttcagta cacatttgca
     7501 agaacctgca gtcttcttag aaaagcagat attgatataa atgatgatgt ggattattct
     7561 gttttgacag acagtgaatc attcaatgta attaaaaaac tggaaggttt taaagaggcg
     7621 gttttaaacg ctcatgataa atacgagccc tttatgatta cccgatatat tgtcggcctt
     7681 gcacaagaat ttaaccgatt ctatcatgaa tgtcagatta ttactgacga tgaaagtgtt
     7741 aaaaaggcaa gactgcttct tactctcaca gtcaatacag ttcttaaaca aggtatgaag
     7801 cttctgggaa tggaagcacc ggagaagatg taactacgta aactgcggtg ccgggcacct
     7861 ttaggtgcct ggcaccgatt ttttttacta aaacgtcaaa ttaactccct attcaacaat
     7921 acatttcaaa tttcaacatt gagcataatt taaatttaca ttataattaa tattggacaa
     7981 ggaggtacga aatgaaaagg gtaaatt
//
LOCUS       DAOSOW010000028         7960 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_9072_length_7960_cov_2.967236, whole genome shotgun sequence.
ACCESSION   DAOSOW010000028 DAOSOW010000000
VERSION     DAOSOW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9072_length_7960_cov_2.967236"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /gene="ybeY"
                     /locus_tag="PLD67_01455"
     CDS             <1..400
                     /gene="ybeY"
                     /locus_tag="PLD67_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOT21224.1"
                     /translation="KDNEDAIKNAVDAVLAEEGLHGDFEVSVSFVTNEEIKELNREYR
                     NVDSETDVLSFPMDEVFDGVTILGDIVISTQKIIEQANDFNHSLEREMCYLTVHSMLH
                     LLGYDHMNKEEKNKMRAKEKEIMKKLKIFK"
     gene            420..1484
                     /locus_tag="PLD67_01460"
     CDS             420..1484
                     /locus_tag="PLD67_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HOT21225.1"
                     /translation="MKTKIAVLIICLLFISACAKETVTPEQAEPEINEEEPEEKPEVD
                     KEEIEEEIDPNKVLSPLDGLRYYPEELSKRPVAVSIDNHHRARWQAGISQAEIVYEVE
                     VEHPFTRYLCIFLAHEPEMIGPVRSARPYMIYYALENDGIFVHVGGSEDAFSEIKRLK
                     VADVDGLYSGAMWRYNDTGKYAPHNVYTTLESIRNEAKAYGYRDEGNFEGYLFNEKDV
                     KLSENFETTPSKEVNIVYNYYNTTDYIFNEENNLYLRFKDDEEHIDELDKKQLTAKNI
                     IAVETSKSILDNEGRLFLGTIGKGKGIYITNGESIPIIWEKTSEKDRLKFYSGDEELM
                     LNPGNTWIQVVNSLENITLR"
     gene            1526..1918
                     /gene="cdd"
                     /locus_tag="PLD67_01465"
     CDS             1526..1918
                     /gene="cdd"
                     /locus_tag="PLD67_01465"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOT21226.1"
                     /translation="MEDKLLIKIAMEAREASYSPYSNYKVGAAVYTKSGKVFTGCNIE
                     SASFTPTICAERVAVSKCISEGYRDIYKIAVVGSEIKTSFPCGVCRQFLAEFGKDIKV
                     ICARNIDSYDVYTLEELLPNSFGPDDLA"
     gene            1931..2818
                     /gene="era"
                     /locus_tag="PLD67_01470"
     CDS             1931..2818
                     /gene="era"
                     /locus_tag="PLD67_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOT21227.1"
                     /translation="MFKSGFVTIIGRPNVGKSTLMNNMLGEKISIMSDKPQTTRNKIR
                     TVYTDDEAQIIFIDTPGIHKPKNQLGEFMNTEVDSALEEMDVLIMITDEFNKVGPGDE
                     FIIEKIKDIKAKKILIINKIDKFDKEAALRIAAEFDKYKVFDDIVPISALNNVGVDAL
                     VKLIIKYLKPGPMYFPADYITDRPERFIVSEIIREKALMYLEDEVPHGIAVEIEEMKE
                     RKDRDLVDIRANILCEKKSHKSIIIGKEGRKIKGIGKSAREEIERLLGSQVNLQLFVK
                     ISENWRDDSYRLKTLGYDK"
     gene            2815..3528
                     /gene="recO"
                     /locus_tag="PLD67_01475"
     CDS             2815..3528
                     /gene="recO"
                     /locus_tag="PLD67_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOT21228.1"
                     /translation="MIQEEILILKEISYKENDKILHALSRTRGKIRIISKGSKKSSSH
                     LINASQIFAYSTCTLNVSKDMYILTSAKLLDNFYNLNKNMAAYYNASYILELISYVAQ
                     ENEVDSRVFDMTVSVISHLSNCTYEHEKLTAAYELKLAAMLGYKPDFLHCLRCGSSIN
                     DESKFSVTEGGVFCSKCVNFGNGINVTYNEILIMDKILKSKFENIGFIEIPKTIMNLI
                     RKFLFYYIGKDNFTTLKLL"
     gene            3545..4147
                     /locus_tag="PLD67_01480"
     CDS             3545..4147
                     /locus_tag="PLD67_01480"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HOT21229.1"
                     /translation="MENDTLNKIDEIIKRTNTDYGTAKEALEAANGDVLEAIILIENQ
                     QKGSTGSFRGEQIMAQLKDILAKGNATKLTIKKDNEVIVNIPITAGLVSAFIAPFLSA
                     AGITVALLAKCSVEITQTDGKIIDLGQKVDEGIDAVKDAMHDIKKGAQNLKDDLRTGS
                     KNIKDDIKTGAENVKDNIKAEAENMKDDLKNSFDRNNVHF"
     misc_binding    4191..4368
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4474..5868
                     /locus_tag="PLD67_01485"
     CDS             4474..5868
                     /locus_tag="PLD67_01485"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOT21230.1"
                     /translation="MAKIEKNSLEKVVSLCKSRGIIFPGSEIYGGLANTWDYGPLGVE
                     IKNNIKRAWWKKFIQESPYNVGLDAAILMNPTTWVASGHVGGFSDPLMDCKECKERFR
                     ADKLIEDFMKEIDDVQVVDGWTNDEMEAYVAEKKVKCPNCGAHNFTTIRKFNLMFKTF
                     QGVTEDSQSELYLRPETAQGIFVNFKNVQRSMRKKIPFGIGQIGKSFRNEITPGNFTF
                     RTREFEQMELEFFCKPGEDMEWFYYWKEFCMNWLLNLGMNKEKLRFRDHEPAELSHYS
                     KGTSDIEYLFHFGWGELWGIANRTDFDLKQHINTSGEDLYYQDPITNEKYIPYCVEPS
                     LGADRVMLAILVDAYNEETLEDGTDRVVLKLHPALSPYKAAIFPLTKKLNEQAIELYQ
                     MLNKHFNVDYDDAGSIGKRYRRHDEIGTPYCITFDFDSLEDNTVTVRDRDTMEQVRIK
                     IDDLVTFIDEKTSF"
     gene            5935..6936
                     /locus_tag="PLD67_01490"
     CDS             5935..6936
                     /locus_tag="PLD67_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOT21231.1"
                     /translation="MNIREKYEQFEIEHLSEYASKAALSKGRMLYEPKCEIRTDFQRD
                     RDRILHSKAFRRLKHKTQVFLSPEGDHYRTRLTHTLEVSQISRTIARSLNLNEDLTEA
                     IALGHDLGHTPFGHTGERILKELCNGFKHNEQSLRVVDFLEVRNERQGLNLTFEVRDG
                     ILNHPSGYKPATLEGQIVSISDRIAYINHDIDDAVRGHIITKIPDKFLKNLGSSHGQR
                     INTMIMDIIQNSENKNKIVMSDEVAELTDELRNFLFENVYYNKVAKSEEDKTMFIVEK
                     IFEYYTNNFEQLPEFYINIYNNNNFSKGEIIKDYIAGMTDRYAMKVFEELYIPKPWI"
     gene            7068..>7960
                     /gene="dnaG"
                     /locus_tag="PLD67_01495"
     CDS             7068..>7960
                     /gene="dnaG"
                     /locus_tag="PLD67_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOT21232.1"
                     /translation="MPYRLDSDVIQRILEQNNIVDIIEEYLPLKKAGANYSTTCPFHK
                     EKTPSFIVSPDKQIFHCFGCGESGDSISFLTKYKNYTFVEAAEYLANRAGIILEEINS
                     SRINNNKQTADMLYKINRDAAAYFYKNLRKSPNAIKYLFSRNIKSDVIKSFGIGYAID
                     EWDNLLNYLKNEGYNEEDILKTGLIIWNDKKNTYFDRFRNRIIFPIFDIKKRIIGFGG
                     RVIDDSMPKYLNSPESLIFNKGYNLYGIHIAKENIRNNSFILVEGYMDVIKMHAHGYK
                     TAVAALGTSFTENQAKLLKRYS"
BASE COUNT         3046 a         1094 c         1602 g         2218 t
ORIGIN      
        1 caaagacaat gaagatgcaa taaaaaatgc tgtagatgca gttttggcgg aggaaggact
       61 gcatggagat tttgaagtaa gcgtttcttt tgttacaaac gaggaaataa aggagttaaa
      121 cagggaatat agaaacgttg acagtgaaac agatgtttta tcttttccca tggatgaagt
      181 atttgatgga gttaccattc ttggagacat tgttatttca acacaaaaaa ttattgaaca
      241 agctaatgat tttaatcaca gcctggaaag agagatgtgt tatctgactg tacacagtat
      301 gctgcatcta ttgggatatg accatatgaa taaagaagaa aagaataaga tgagagcaaa
      361 agaaaaagaa attatgaaaa aattaaaaat attcaaataa tacagccgag ggggaagacg
      421 tgaaaacaaa aatagctgtt ttaattatat gcttgctttt tatcagcgca tgtgcaaaag
      481 aaactgttac acctgaacaa gcggaacctg aaataaatga agaagaaccg gaagaaaaac
      541 cggaagtcga taaagaagaa atagaggaag aaattgatcc aaataaggta ctgtcaccat
      601 tggacgggct aagatactat cctgaagaat tgtcaaaaag accggtagcc gtatctattg
      661 acaaccatca cagggcaaga tggcaggcag gtattagtca agccgaaata gtatatgaag
      721 ttgaagtaga gcatccattt accagatact tatgtatatt tcttgcacat gagccggaaa
      781 tgataggacc tgtacgaagt gcaagaccat atatgatata ttatgcactt gaaaatgacg
      841 gaatatttgt gcatgtggga ggaagcgaag atgctttttc agaaataaaa agacttaaag
      901 ttgcagatgt agacggactt tattcaggag ctatgtggag gtacaacgat accggaaaat
      961 atgcccctca taatgtgtac acaacattgg aatcgattag aaatgaagca aaggcttacg
     1021 gatacagaga tgaaggaaac tttgaaggat atttgttcaa tgaaaaagat gtaaaattat
     1081 cggaaaactt tgaaaccact ccgtcaaaag aagtaaacat agtatataat tattataata
     1141 ctacagatta catatttaat gaggaaaaca atttatattt aagatttaaa gacgatgaag
     1201 aacatattga tgaattggat aaaaagcaat taactgcaaa aaatattatt gcagtagaaa
     1261 cttccaaatc aatattggat aatgaaggaa gattattctt ggggaccatc ggcaaaggaa
     1321 agggcattta tatcacaaac ggagaaagta ttcctataat atgggaaaaa acttcagaga
     1381 aagacagact gaaattttat tcgggtgatg aagaacttat gttgaatccg ggcaacacat
     1441 ggatacaggt tgttaacagc cttgaaaata taactttaag gtgacaggag aagtatcgaa
     1501 ataaagaaat gagaaagggg acaaaatgga ggataaatta ctcataaaaa ttgccatgga
     1561 agcaagggaa gcgtcatata gtccatattc caattataag gtaggtgctg cagtttatac
     1621 aaaaagcggc aaggtattta caggctgcaa tatagaatca gcatctttta ctcccaccat
     1681 atgcgctgaa agagtagctg tttcaaaatg catttctgaa ggttataggg atatttacaa
     1741 gattgccgtg gttggaagcg aaataaaaac atcttttcct tgcggagtat gcaggcagtt
     1801 tttggcggaa ttcggcaagg atataaaggt tatttgtgca agaaacatag attcttatga
     1861 tgtttatacc ttagaggagc ttttgccaaa cagcttcgga cccgatgacc ttgcctaaga
     1921 aaggaaaatt atgtttaaat caggatttgt aactataata ggaagaccaa atgtgggtaa
     1981 atcaacatta atgaataata tgttaggtga aaaaatatcc ataatgtccg ataaaccaca
     2041 aacgaccaga aataaaataa gaacagtata tacggatgat gaagcacaga taatatttat
     2101 agatactccg ggaattcaca aaccaaaaaa tcaacttgga gaatttatga atacggaagt
     2161 ggactcagca ttagaggaaa tggacgtatt aataatgata actgatgagt ttaacaaagt
     2221 gggacccgga gatgaattta taattgaaaa aatcaaagac atcaaagcga aaaaaatact
     2281 cataattaat aaaattgata agtttgataa ggaagctgcc ttgcgtatag cggcagaatt
     2341 tgataagtat aaggtctttg atgatatagt gcctatttct gcccttaata atgttggcgt
     2401 agatgcattg gttaaactga taattaaata tttaaaaccc ggaccgatgt attttcctgc
     2461 agattatata accgacaggc cggagcgttt tattgtttct gaaattataa gagaaaaggc
     2521 cctcatgtat ttagaggatg aggtaccgca cggaattgct gttgaaattg aagagatgaa
     2581 ggaaagaaaa gaccgagacc ttgtagatat aagagccaat attttatgcg agaaaaagtc
     2641 tcacaaaagc attattatcg gcaaggaagg aagaaaaata aagggtatag gcaaaagtgc
     2701 aagggaagaa atcgaaaggc ttttggggag ccaggtaaat ttacagcttt tcgttaaaat
     2761 ttccgaaaac tggcgtgatg attcttatcg tcttaaaact ctagggtatg ataaatgata
     2821 caggaagaaa tcctaattct aaaagaaata agttataaag aaaatgataa aatacttcat
     2881 gccttgtcaa gaacaagagg taaaattcgg attatttcaa aaggctcgaa aaaaagcagc
     2941 tcacatctga taaatgcatc gcagattttt gcatactcaa cgtgcacact caatgtatca
     3001 aaagatatgt acatattaac ttccgccaaa cttttggata atttctacaa cttaaacaag
     3061 aacatggctg catattataa cgcatcttat atattagagc ttataagtta tgtagcacag
     3121 gaaaatgaag ttgattccag agtatttgat atgacggtat ctgtgatttc tcatttaagc
     3181 aattgcacat atgaacatga aaagcttact gctgcctatg aattaaaatt ggcggcaatg
     3241 cttgggtaca agcctgattt tctccattgc cttcgttgcg gctcttcaat aaacgatgaa
     3301 agtaagttct ccgtaacaga aggaggggtt ttttgctcta aatgcgtaaa ttttggaaat
     3361 ggaataaatg taacatataa tgaaatacta attatggata aaattttaaa atctaaattt
     3421 gagaatattg gctttataga aatacctaag actataatga acttaataag aaaatttttg
     3481 ttttattata tagggaagga taattttact acattaaagc tattgtaaaa gaaaggatgt
     3541 tcatatggaa aatgatactt taaacaaaat cgacgaaatt ataaagcgca caaatactga
     3601 ttacggtaca gcaaaagaag ctttggaagc tgcaaacggt gatgtgcttg aagctataat
     3661 actgattgaa aatcagcaaa aaggcagcac gggctccttt agaggagagc agataatggc
     3721 tcaactgaag gatatattag ctaaaggaaa tgctacaaaa ctcacaatta agaaggataa
     3781 tgaagttata gtcaatatac cgataactgc aggcttggta agtgctttta ttgcgccttt
     3841 tctttctgca gcaggaataa ccgtagcact gcttgcaaaa tgctctgtgg aaataacaca
     3901 aacagacggt aaaataatag atctcgggca aaaagttgat gaaggaatcg atgctgtaaa
     3961 agatgctatg catgatataa aaaaaggtgc acagaattta aaagatgatt taagaacagg
     4021 ctcaaaaaat attaaggatg atataaaaac aggtgccgaa aatgtgaagg ataacataaa
     4081 ggccgaagct gaaaatatga aagatgattt aaaaaattct tttgacagaa ataatgtgca
     4141 tttttaaaaa gctcttgaca taaaaagatt attttgttac attatagtaa attaagacta
     4201 tgaaaaagaa gagtattcta tgcttaattt acagagagtc agataaggtg aaaactgata
     4261 aggatgtata ggagaaggga gcttttgagt ctatactttt gagaggatga agctttagtg
     4321 ctttgtcaaa tagggtggaa ccgcggaagt aaatctttcg tccctatatg ggatgagaga
     4381 ttttttatgt tctcttccat ttataaggaa tggtacgcct gcgtactttt aatatattta
     4441 taacttaaaa ttattataca tatggaggaa tttatggcaa agattgaaaa aaacagctta
     4501 gaaaaagtag tatcattatg taaatcaaga ggaattattt ttccgggctc tgaaatttat
     4561 ggcggcttag ctaacacatg ggattacgga cctttgggag tagagattaa gaacaacata
     4621 aaaagagctt ggtggaaaaa atttatacaa gaaagcccat ataatgtagg attagatgca
     4681 gcaatactta tgaatcctac aacttgggtt gcatccggtc acgtgggagg attttcagac
     4741 ccattgatgg actgtaaaga gtgtaaggaa agattcaggg ctgataagct tattgaagat
     4801 tttatgaaag aaatagatga cgtacaagtg gttgatggtt ggacaaacga tgaaatggaa
     4861 gcatatgttg cagaaaaaaa agtaaaatgt cctaattgcg gggcacataa ttttacaact
     4921 ataagaaaat tcaatttaat gtttaaaact tttcaaggtg taaccgaaga ttctcaatcc
     4981 gagctttatt taagacccga aacagctcag ggtatttttg ttaactttaa aaatgttcag
     5041 cgctccatgc gaaagaaaat tcctttcggt ataggacaaa taggaaaatc attcagaaat
     5101 gaaattacac cgggtaactt tacattcagg actagagaat tcgagcaaat ggagttggaa
     5161 tttttctgca aacctggtga agacatggaa tggttctact actggaaaga attctgtatg
     5221 aactggcttc taaaccttgg aatgaataag gaaaaactac gtttcagaga ccatgaaccg
     5281 gctgaattgt cacattacag taaaggtaca tcagacattg aatatttgtt ccactttggc
     5341 tggggagaat tatggggcat tgcaaacagg actgattttg acctgaaaca gcatataaac
     5401 acatccggag aggatttata ttatcaggat cctataacca atgaaaaata cataccatac
     5461 tgtgtagagc catccttagg agcagaccgt gtaatgctgg caattctggt tgatgcatat
     5521 aatgaagaaa cgctagaaga tggaaccgat agggttgtgt taaagcttca ccccgcattg
     5581 tcaccatata aagcggcaat attcccgctt acaaaaaaat taaacgagca ggctatagag
     5641 ctttatcaaa tgcttaacaa gcattttaat gtagattatg atgacgcggg aagtataggg
     5701 aaaagataca gaagacatga tgaaatagga actccttatt gcattacatt cgattttgat
     5761 tcattagaag acaatacagt taccgtaagg gatagagaca caatggagca ggttagaatt
     5821 aaaattgatg atttagtaac atttattgat gaaaaaacat ccttttaaag atgctttccc
     5881 catttttttg agtgtttttt ctgtttacaa tccagaattc tttggaggtg tattttgaat
     5941 atcagagaaa aatacgagca attcgaaata gagcatctgt cagagtatgc ctccaaggca
     6001 gcactctcaa aaggaagaat gctttatgag ccgaaatgtg aaataagaac ggattttcag
     6061 agagacagag acaggatttt gcattcaaaa gctttcagaa gacttaaaca taaaacacaa
     6121 gtttttttat ctcctgaagg tgaccactac agaacaaggc ttacgcacac gcttgaagta
     6181 tcgcagatat caagaacaat agcgagaagc ttgaatttaa acgaggattt aacggaagct
     6241 attgcccttg gacatgattt aggccataca cctttcggtc acacgggaga aagaatactt
     6301 aaagagctat gcaacggatt taaacacaat gagcaaagtc taagagtcgt ggattttttg
     6361 gaagtaagaa atgaaaggca agggcttaat ttgacttttg aagttagaga cggtatattg
     6421 aatcatcctt cggggtataa acctgctacc ctggaggggc aaatagtaag catttcagac
     6481 agaattgcat atataaatca tgatattgat gatgcggtca ggggacatat tattactaaa
     6541 attccggaca aatttctaaa gaatttaggc tcaagtcacg gtcaaagaat taacacaatg
     6601 ataatggaca taattcaaaa ctcggaaaac aaaaataaaa ttgtaatgag cgatgaagtt
     6661 gccgagctga cggatgagct tcgtaatttt ctgtttgaaa atgtatatta taataaagtt
     6721 gccaaaagcg aagaagataa aacaatgttc atcgttgaaa aaatatttga atattacact
     6781 aataattttg aacaacttcc tgaattctat attaatattt ataataataa taacttcagc
     6841 aagggagaaa ttataaaaga ttatatagcg ggtatgacag acagatatgc aatgaaggtt
     6901 tttgaagaac tgtatatacc taaaccctgg atataaaaaa taaaaaaaga agcagaaaat
     6961 actatattac aagaatcaga aaattttagg gcttgtcaaa aaaaatattg acaagcttat
     7021 tttcctatgt tatactttat tttcaatgta aggataatgg tgatattatg ccctataggt
     7081 tggattctga cgttatacaa agaatcttgg aacaaaataa tattgtagat ataatagaag
     7141 aatatttacc tttaaaaaaa gcaggtgcaa attacagcac aacctgtcct tttcataaag
     7201 aaaaaacacc ttcatttata gtttctccgg ataagcaaat tttccattgc tttggctgcg
     7261 gtgaaagcgg agatagtata agttttctga caaaatataa aaattataca tttgttgaag
     7321 cagctgaata tttagcaaac agagcaggta ttatcttgga agaaattaac tcatcaagaa
     7381 ttaacaataa taaacaaaca gcagatatgc tatacaaaat taacagagat gctgctgctt
     7441 atttttacaa gaatttaagg aagagtccta atgcaattaa atatttattt tccagaaata
     7501 ttaaatcaga tgtaataaaa tcgtttggaa ttggatatgc tattgacgaa tgggataatt
     7561 tgttaaatta tctaaaaaat gaaggttata atgaagaaga tattttaaag accggattaa
     7621 ttatttggaa cgataaaaaa aatacatatt ttgatagatt tagaaacaga ataattttcc
     7681 ctatatttga tattaaaaaa agaattatag gttttggggg aagagtaata gatgactcta
     7741 tgccgaaata tcttaattct cctgaaagcc ttatttttaa taagggatat aatttatacg
     7801 gaattcatat agcaaaagaa aatataagaa ataacagctt tattttggtt gaaggatata
     7861 tggatgtaat caagatgcat gcccatggtt ataaaacagc agttgcagca ttaggcacat
     7921 cttttaccga aaaccaagca aaactgttaa aaagatactc
//
LOCUS       DAOSOW010000029         7930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_9116_length_7930_cov_4.786286, whole genome shotgun sequence.
ACCESSION   DAOSOW010000029 DAOSOW010000000
VERSION     DAOSOW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9116_length_7930_cov_4.786286"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PLD67_01500"
     CDS             complement(<1..420)
                     /locus_tag="PLD67_01500"
                     /inference="COORDINATES: protein motif:HMM:NF015644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOT21233.1"
                     /translation="MRNKGRLFVLGIVVIVFLIINSFTNIVEFITNYLWFKEVGYTQT
                     FFTKIKAQIMIGIPIFIILSILLYIFIKKLKKKYDDETEIIVVNKKFNLTIKLISAGA
                     SLLITLNIISTMWFEILQYINGVEFGATDPIFNNDVSF"
     gene            complement(432..1193)
                     /locus_tag="PLD67_01505"
     CDS             complement(432..1193)
                     /locus_tag="PLD67_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004819636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HOT21234.1"
                     /translation="MIEPSKNSKRIKNYDLFLLYHETKDIEIRNQIFEKYKYMAEIIS
                     RKYSNRGIEHDDIYQIACMGLIFAIERFDISKGFEFTSFATPTILGEIKKYFRDKGWA
                     VKVPRKIQEISRKVNEYNNILTARLHRAPTIKEIAEYISSTEEEVLEAFEAGRMFNSQ
                     SLDEKFDLSRDDNDISLMDVVGAEDKHYNLIENLDFLNKSMSKLNELEKNIIIKRFYE
                     NKTQSDIAKELKISQMTVSRIEKKSLEKLRIEYYR"
     gene            complement(1195..1569)
                     /locus_tag="PLD67_01510"
     CDS             complement(1195..1569)
                     /locus_tag="PLD67_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOT21235.1"
                     /translation="MDKILLKIPKKSEYMSTIRLTSSSLFNLNGFNIDEIEDIKVIIS
                     EICTFFIKNLSRSTEDFEISYQVFKNKIIVTVADLNDGELSDESLNNDMSILIIESLS
                     DNFNYDLNNKTITFEKNKIGVD"
     gene            complement(1574..1885)
                     /locus_tag="PLD67_01515"
     CDS             complement(1574..1885)
                     /locus_tag="PLD67_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOT21236.1"
                     /translation="MSLEIESKIQDSIIEIKPIGEVDIYTSPELKNQIFSLIEERNTS
                     IIIDGESLEYIDSTGLGVLMSIYKKMQENSLNLEIINLKPNIYKLFDITGLNKVFNIR
                     E"
     gene            complement(1914..4352)
                     /gene="gyrA"
                     /locus_tag="PLD67_01520"
     CDS             complement(1914..4352)
                     /gene="gyrA"
                     /locus_tag="PLD67_01520"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOT21237.1"
                     /translation="MSENVNKNKIVNINISEEMKRSYLDYAMTVIVSRALPDVRDGLK
                     PVHRRILYSASELGLSPDKPHKKSARIVGDVLGKYHPHGDTAVYDAMVRLAQSFSMRY
                     PLIDGHGNFGSIDGDEAAAMRYTEARMSKIAMEMLRDFNKGTVDFRPNFDETLKEPVV
                     LPSRFPNLLVNGSSGIAVGMASSIPPHNIGEVINGIIAYIENPDIDIPGLMRYIKGPD
                     FPTGAIIEGKDNIKNAYLTGRGILKVKAKAEIEELKNNKSQILVSELPYQVNKARLIE
                     KIADLVKDRIIEGISDIRDESDRDGMRIVIEIKRDYNPNIVLNQLFKHTQMQDSFSVI
                     MIALVNNEPKVLNLKEMIYHYVEHQREVIVRRTKYDLDKAEARAHIVEGLLIAIDNID
                     EVIKIIRASYDDAEKRLMEAFNLTEIQARAIVDMRLRRLQGLEREKLNVEYEDLIKMI
                     NKFKEILSNQNLIDQIIKDELKEIKNSFYDERKTKITADEGEINVEDLIEEENVAITL
                     TNFGYIKRLPEDTYKVQNRGGKGITALTTRDEDFVKDLFITSTHDYLMFVTNKGKIYK
                     IKAYEVPEARRQAKGTAAINLINIEPGEKIKSLIPIREFNDNEFLIFVTKKGIIKKTK
                     LSEFESNRKTGLVAIKIAEDDELISVNKTDGTKDIFIVTKQGKGIRFHESDVRETGRN
                     TMGVKAITLSKNDELVAMSIIRKSDENKHILTVTENGYAKITLSQEYRPQSRGGKGMK
                     AHNINKKTGLIVNSLIVDKEDDIMILSTSGTIIRMHARDISIMGRDTQGVIAMRLKGD
                     DKVASIAKIFSEED"
     gene            complement(4368..6281)
                     /gene="gyrB"
                     /locus_tag="PLD67_01525"
     CDS             complement(4368..6281)
                     /gene="gyrB"
                     /locus_tag="PLD67_01525"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOT21238.1"
                     /translation="MATDKNNNHYSADQIQVLEGLEPVRKRPGMYIGSTGERGLHQLV
                     YEVVDNSIDEAMAGYCDTIKLTINEDNSVVVVDNGRGIPIETNKKTGKSTLETVLTVL
                     HAGGKFDNDAYKVSGGLHGVGVSCVNALSEYLVAEVNWDGKKYRQRYERGIAKTEVEF
                     IGNTNKTGTSITFAPDPTIFDTTVFNYTTIKHRMRELAFLNKGIKIILEDKRQKIKNE
                     FHYEGGIREFVQFLNSKKEPLHNDVIYTEGTKDKVNVEIAIQYTDSYNENIFSFVNNI
                     NTIEGGTHLVGFKTALTRVINDYAKKANLIKENEVGITGEDIREGITAILSIKITNPQ
                     FEGQTKTKLGNSETRGIVDSITGEALSIYCEENPKTAKAIVEKALKSARAREAARKAR
                     DLVRRKGALDSMALPGKLADCSEKDPALCEICIVEGNSAGGSAKEGRDRRTQAILPLR
                     GKILNVEKSRIDRILNSEEIKNMITAFGCGIGEDFDISKLRYHKIIIMTDADVDGAHI
                     RTLLLTFFYRYMKELITGGYVYAAQPPLYRVRKGKFEKYVYSDDELNQVLDEIGRDNK
                     NEIQRYKGLGEMDPEQLWETTMNPENRILLRINEDDAIEADEIFNVLMGDKVAPRREF
                     IEENAKYVQNLDI"
     gene            complement(6293..7408)
                     /gene="recF"
                     /locus_tag="PLD67_01530"
     CDS             complement(6293..7408)
                     /gene="recF"
                     /locus_tag="PLD67_01530"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOT21239.1"
                     /translation="MIVNKLIIKNYRNFKEAEAELNNSLNIFIGDNGQGKTNLMEAIY
                     IASIGRTFRLNSENELINFFENKSSVEIYIEKNNYKIKIEILLEKNKKKQVIINGVKL
                     EKTSEMIGILNNVIFTPDDMKIIKGSPVERRRFINIDISQIKPKYKYLLNKYKKVTTE
                     RNLLLKNYYTKCENKDIISIWNDYLVNTGTEISLYRNEYINKLKKAATDIYSSISGNK
                     EKLDILYLSNIGCITDRDEVKKIFYDKINKNFNNEILKGISLYGPHKDDLIIKINGKE
                     CKYFGSQGQQRSVILALKLAEIEIIKEETGEYPILLLDDVLSELDNKRKSYLIEHIKD
                     IQTIITSTDEHELNILTKNFDKKMFYINEGKISNISN"
     gene            complement(7455..7667)
                     /locus_tag="PLD67_01535"
     CDS             complement(7455..7667)
                     /locus_tag="PLD67_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOT21240.1"
                     /translation="MEKININTEYIKLDQLLKYTNAVEGGGMAKNVILDGLVKVNGEV
                     VLQRGKKLREGDIIEFNNEKYLIERY"
     gene            complement(7756..7920)
                     /locus_tag="PLD67_01540"
     CDS             complement(7756..7920)
                     /locus_tag="PLD67_01540"
                     /inference="COORDINATES: protein motif:HMM:NF014787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21241.1"
                     /translation="MGFNSKYILDYLKVIDSEMISLNLIGKNNPCFIKEENEENKDNY
                     IYMVLPVRIS"
BASE COUNT         2169 a         1562 c          982 g         3217 t
ORIGIN      
        1 aaaactcaca tcattattaa agatagggtc ggttgctcca aattccacac cgtttatata
       61 ttgaagaatt tcaaaccaca tggtggatat tatattcaat gttataagaa gacttgctcc
      121 tgcagaaatt aatttaatag ttaaattaaa ttttttattt acaacgatta tttctgtttc
      181 atcatcatat ttcttcttta gtttcttaat aaatatgtac aataagatgc tcaatataat
      241 aaaaatcgga ataccaatca taatttgcgc tttaattttg gtaaaaaatg tttgggtata
      301 gccgacttct ttaaaccaca aataatttgt aataaattct actatattgg taaaggaatt
      361 gataatcaaa aaaacgatga caacaattcc cagtacaaat aatcttcctt tattcctcat
      421 attacctcct tttatctgta gtattcaatt cttaattttt ccaaagattt cttttctatt
      481 ctggaaacag tcatttgaga tatttttaat tctttggcaa tatcactttg agttttattt
      541 tcataaaacc tctttataat gatatttttt tcaagttcat tcaatttact catcgatttg
      601 ttcaaaaaat ccaaattttc tattagattg taatgtttat cctcagctcc cacaacatcc
      661 ataagcgata tgtcattatc atccctgctt aaatcaaatt tttcatcaag ggattgagaa
      721 ttaaacattc ttcctgcctc aaatgcttcc aatacttctt cctctgtact gcttatatat
      781 tcggcaattt cttttattgt tggtgccctg tgaagccggg ctgtcaaaat attgttgtat
      841 tcattgactt ttcttgatat ttcctgaatt tttctgggca ccttaacagc ccaaccctta
      901 tctcgaaaat atttttttat ttctcccaat atggttggtg ttgcaaaact tgtaaattca
      961 aatccttttg atatatcaaa tctttctatg gcaaaaatta gccccatgca tgcaatttga
     1021 tatatatcgt catgctcgat acctctgttg ctgtattttc tggatataat ttcagccata
     1081 tatttatatt tttcaaaaat ttgatttctt atttctatat ctttggtttc atgataaagt
     1141 aaaaacaaat cataattttt aatacgcttg gagtttttac tcggttcaat catattaatc
     1201 aacgcctatt ttattttttt caaaagttat tgttttatta tttaaatcat aattgaaatt
     1261 atcggacaaa ctttcaataa tcaatatgct catatcattg tttaaacttt catcggataa
     1321 ttcaccatca tttaaatccg ctaccgtcac tataatctta tttttgaaaa cttgataaga
     1381 tatttcaaaa tcttccgtgc tgcggcttaa attctttata aaaaatgtgc aaatttcaga
     1441 tattattacc ttaatatctt caatttcatc aatattgaat ccgtttagat taaacagtga
     1501 cgatgatgtt aatcttattg tgctcatata ttcggatttt tttggaattt tcaacaatat
     1561 tttatccatt tcatcactcc cggatattaa acactttgtt taaacctgtt atatcaaaaa
     1621 gtttataaat atttggcttt aaattgatta tttccagatt taaactgttt tcctgcattt
     1681 ttttataaat gctcatcaaa acaccaagcc ctgtactatc gatatactct aagctttcac
     1741 cgtcaatgat gatgctggta tttctttctt caatcagtga aaatatttga tttttcagct
     1801 caggagaagt gtatatgtct acttctccta tcggttttat ttctataatg ctatcctgaa
     1861 ttttgctttc aatttccaat gacatatttt ttacctccgt tattcttcct ttttcaatct
     1921 tcctctgaaa aaatctttgc tattgaggca actttgtcgt cacccttcaa tctcattgca
     1981 ataacacctt gagtatctct tcccattatg gaaatatctc ttgcatgcat tctgattatc
     2041 gttccgcttg tacttaaaat cataatgtca tcttctttat caactattag tgaattgact
     2101 ataagccctg tttttttatt tatgttgtgg gctttcatac cttttccgcc cctgctctga
     2161 ggtctgtatt cctgagacag ggttattttt gcataaccgt tttctgttac ggttaaaatg
     2221 tgtttatttt catcgctttt cctaataatg ctcatggcaa caagctcatc gtttttagaa
     2281 agtgttattg ctttaactcc catggtgttt ctgccggttt ctcttacgtc gctctcatgg
     2341 aatcttatac cctttccttg tttcgtaaca atgaatatat cctttgttcc gtctgttttg
     2401 ttaacactta tcaattcatc gtcttctgca atttttatag ccaccaaacc tgtctttctg
     2461 tttgattcaa attcgctgag cttagttttt ttgataatac ccttcttggt tacaaaaatc
     2521 aaaaattcat tatcgttaaa ttctcttatc ggaatcaatg attttatttt ttcgcccggt
     2581 tcaatgttaa tcaagtttat tgcagctgtt cctttagcct gacgtcttgc ttccggtact
     2641 tcatatgcct ttattttata tattttccct ttgtttgtaa caaacattaa ataatcatga
     2701 gttgatgtta taaacaaatc ctttacaaag tcttcatctc ttgtggttag tgcggtaatt
     2761 cccttaccgc ctctgttttg aaccttataa gtatcctccg gaagcctttt aatatatccg
     2821 aaatttgtaa gtgttattgc aacattttcc tcctctatta aatcttctac attgatttct
     2881 ccttcatctg ctgtaatttt tgtttttctt tcatcataaa aactattctt tatttctttt
     2941 aattcatcct ttattatttg gtcaatcaaa ttctgattag atagaatttc tttaaacttg
     3001 tttatcattt ttatcaggtc ttcatattca acatttaatt tttctctttc aagaccttgc
     3061 agacgtctaa gtctcatatc tactatggct cttgcctgta tttccgttaa attaaatgct
     3121 tccattaatc ttttctcggc atcgtcatag gatgctctga taattttaat aacttcatct
     3181 atattatcaa ttgcaatcaa taaaccttca acaatatgtg ctctggcctc agctttatct
     3241 aaatcatact ttgtccttct gacaataact tccctttgat gttctacata gtggtatatc
     3301 atttctttta agtttaaaac ctttggctca ttatttacca aagcaatcat tattacgctg
     3361 aagctgtcct gcatctgagt atgtttgaat aattgattaa gtactatgtt gggattataa
     3421 tctcttttaa tttcaataac aatcctcatt ccgtctctgt cgctttcatc cctaatgtcg
     3481 gaaattcctt caattattct atcctttaca agatcagcta ttttttcaat aagtctggcc
     3541 ttattcacct gatatggaag ttctgatact aatatttggc ttttattgtt ttttaattct
     3601 tctatttcgg ctttagcttt aacctttaat attcctcttc ctgtcaagta agcattttta
     3661 atgttgtctt tgccttcaat tatggcgcct gtgggaaaat caggaccttt tatatatctc
     3721 ataagtccgg gaatatctat atcaggattt tcaatataag caattatccc gtttattacc
     3781 tctccgatat tatgaggagg tatggagctt gccattccta ccgctatacc tgatgaaccg
     3841 tttaccagca agttaggaaa acggcttgga agcacaacgg gctccttcaa agtttcgtca
     3901 aaattcggtc tgaaatcaac cgtaccttta ttaaaatcac gaagcatctc cattgctatt
     3961 tttgacatac gtgcttcggt ataacgcatt gctgctgcct catcaccgtc tatggagcca
     4021 aaatttccgt gtccgtcaat taatggatag cgcatggaga aactttgtgc taaccttacc
     4081 attgcatcat aaacggcagt atctccgtga ggatgatact tacccagaac atctcctaca
     4141 attcgggcac tttttttgtg aggtttatcc ggcgaaagcc ccaattcact tgccgaatat
     4201 aatattcttc tgtgaaccgg tttaagcccg tcccttacat caggcaatgc tcttgatact
     4261 attacggtca ttgcatagtc taaataagac cttttcattt cttcagaaat atttatattt
     4321 acaattttgt ttttattcac attttcgctc atttacaacc tctcagctta tatatctaaa
     4381 ttctgtacgt actttgcgtt ttcttcgata aattctcttc tgggagcaac cttatctccc
     4441 attaaaacat tgaatatttc atctgcttca attgcatcat cttcattaat tcttaataga
     4501 attctgtttt caggattcat ggttgtttcc caaagctgct cggggtccat ttctccaaga
     4561 cccttatacc tttgtatttc attcttattg tctcttccga tttcatccaa tacctgatta
     4621 agttcatcat cgctgtatac atatttttca aattttcctt ttcttactct atacaaaggc
     4681 ggctgggcag cataaacata ccctcctgtt attagctctt tcatatatct gtagaaaaat
     4741 gtaagcagca aagttcttat atgtgctccg tctacgtcgg catcggtcat aattattatt
     4801 ttatgatatc ttaattttga aatatcaaaa tcttctccta ttccacagcc gaatgccgta
     4861 atcatatttt taatttcttc ggaatttaat attctgtcta ttcttgattt ttcaacattt
     4921 aatattttac ctcttaaagg aagtattgct tgagttcttc tgtcccttcc ttcttttgca
     4981 gagcctcctg ccgaattacc ttcaacaata caaatttcac aaagagcagg gtccttctcc
     5041 gagcaatctg ccaatttacc cggtagtgcc atactatcaa gagcgccctt tcttcttacc
     5101 aagtctcttg cttttcttgc agcttctctt gcccgtgcgg attttaaagc tttttctact
     5161 attgctttag ctgttttggg gttttcttcg caatatatac ttaaagcctc tcccgtaata
     5221 ctgtcaacta ttcctcttgt ttcgctgttt cccagcttag tctttgtctg accttcaaat
     5281 tggggatttg ttattttaat cgacaatatt gcagtaatcc cctctcttat atcttcaccg
     5341 gtaattccta cttcattttc ttttattaaa tttgcttttt ttgcataatc atttataacg
     5401 cgtgtaaggg cagttttaaa acctactaaa tgagttcccc cttcaattgt attaatattg
     5461 tttacaaatg aaaatatatt ttcattatag gaatcggtat attgaattgc aatctctaca
     5521 tttactttat cctttgttcc ctcggtataa ataacatcgt tgtgaagagg ttccttcttg
     5581 ctgtttaaaa attgtacaaa ctccctgatt cctccttcat aatggaactc attcttaatt
     5641 ttttgtcttt tatcttcaag gattattttt attcccttat ttaaaaatgc caactctctc
     5701 attctatgct taatagttgt ataattaaat actgttgtat caaaaattgt agggtcagga
     5761 gcaaatgtaa tacttgttcc ggttttattt gtattaccta taaattcaac ctctgttttt
     5821 gcaatacctc tttcatacct ttgcctgtac tttttgccgt cccaatttac ttcagcaacc
     5881 aaatattcag atagagcatt tacgcatgaa acaccaactc catgaagtcc tcccgatact
     5941 ttataagcat cattgtcaaa tttaccgcct gcatgaagta ctgtcaaaac agtttccaag
     6001 gttgatttgc ctgtcttttt atttgtttcg atagggatgc ctctcccatt gtctacaacc
     6061 acaactgaat tatcttcatt aattgttaat ttaatggtat cgcaataccc ggccattgct
     6121 tcatctatgc tgttgtctac tacttcataa accagctggt gaagtcctct ttctcccgta
     6181 gagccgatat acattccggg tctttttctt acaggctcca aaccttcaag aacctgaatt
     6241 tgatctgcgc tgtaatgatt gttattttta tctgtcgcca tttatttctc ctctaatttg
     6301 aaatgttact aatttttcct tcgtttatat aaaacatttt tttatcgaaa tttttagtta
     6361 aaatatttag ctcgtgctca tcagtacttg ttattattgt ttgtatatct ttaatatgct
     6421 caataagata gctctttctt ttattgtcaa gttcagataa tacatcatcc aataataaaa
     6481 taggatattc gcctgtttct tcctttatta tttcgatttc ggcaagcttt aatgctaata
     6541 ttacagagcg ctgctgccct tgagagccaa agtatttaca ttccttaccg ttgattttta
     6601 taattaaatc gtccttatga ggaccgtaaa gggatattcc ctttaaaatt tcattgttaa
     6661 aatttttgtt gattttatca taaaatattt tctttacttc atctctgtcc gtaatacatc
     6721 caatgttgct taaatataat atgtccaatt tttccttgtt tcccgatata cttgaatata
     6781 tatccgttgc tgcttttttt aatttattta tgtattcatt tctgtaaagc gatatttccg
     6841 tacccgtatt caccaggtaa tcgttccaaa tactaataat atccttattt tcacattttg
     6901 tatagtagtt ttttaacaat agatttcttt cagtggtaac ttttttatat ttgttaagca
     6961 aatatttata tttaggcttg atttgtgaaa tatcaatatt aatgaatctg cgtctttcaa
     7021 caggacttcc ctttataatt ttcatatcgt cgggcgtaaa aataacatta tttaaaatac
     7081 ctatcatttc agatgttttt tcaagcttta caccatttat aataacttgt ttctttttat
     7141 ttttttccaa taatatttct attttgatct tgtaattatt tttttcaata taaatttcaa
     7201 ctgaactttt gttttcaaaa aagttaatca attcattttc gctgttcaat ctgaaagttc
     7261 tccctatgga agcaatataa attgcctcca taagattagt cttaccttgt ccgttgtctc
     7321 cgataaaaat attaagggaa ttatttaatt ctgcttcagc ttctttaaaa ttcctgtaat
     7381 ttttaattat aagtttattt actatcattc ttcctcatca gtatccccat aacttgcttg
     7441 gtatgtacct atacctaata acgctcaatt aaatactttt cattattgaa ttcaattata
     7501 tctccttctc ttaacttttt tcctctttgc agaacaacct ctccgttaac cttcactaat
     7561 ccgtccaata tgacattttt agccatacct cccccttcaa cggcatttgt atattttaac
     7621 agctggtcta atttaatata ttccgtatta atgtttattt tttccataca ataccctttc
     7681 taattattct gaaggctagc ctgtagaatc tcatgaaatc cctcgctacg ctcaggatga
     7741 caaacaggaa acctgttaac tgattctcac cggcagaacc atgtaaatat aattatcctt
     7801 attttcttca ttttcttctt ttataaaaca tggattattt tttcctatta aattcaatga
     7861 aatcatttca ctgtctatta ctttcaaata atctaaaata tattttgaat taaaacctat
     7921 ttttatatct
//
LOCUS       DAOSOW010000030         7852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_9228_length_7852_cov_4.168655, whole genome shotgun sequence.
ACCESSION   DAOSOW010000030 DAOSOW010000000
VERSION     DAOSOW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7852
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9228_length_7852_cov_4.168655"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="PLD67_01545"
     CDS             complement(<1..124)
                     /locus_tag="PLD67_01545"
                     /inference="COORDINATES: protein motif:HMM:NF019199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HOT21242.1"
                     /translation="MAEIKCTVTNCYFNKKLGCTAPALTVNGRNADESRSTSCHT"
     gene            complement(187..1254)
                     /locus_tag="PLD67_01550"
     CDS             complement(187..1254)
                     /locus_tag="PLD67_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HOT21243.1"
                     /translation="MRKRWFFLWLIPVSVLINLFVKNNANYAEFYAVNIYPFFVKSVG
                     RLSFITRNSAAELIIIALIVTIIILTALVTIGSIKYRTFEYIKKYIFGVASFISVMYF
                     LFVLFCGINYYRYEFTHYSGLEIQMSSKQELIELCELLIGDANECRAKLETHEGTAKL
                     SDKSYYTTAERAGDFMENLSEEYEILKGNFSAPKPVRQSKIMSYFKITGVFFPYTFEA
                     NVNVHIPPYQIPFVMLHELVHLRGFMREDEANFISYLACIKSGYDDFYYSGTMSALTH
                     SMNALYSVDYDEFVRLYNLYSDDVRKDIDFSIRYWKQFDTKVADAANKVNDIYLKAHN
                     QQDGVRSYGRMVDLLLAYYRQ"
     gene            complement(1556..1756)
                     /locus_tag="PLD67_01555"
     CDS             complement(1556..1756)
                     /locus_tag="PLD67_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOT21244.1"
                     /translation="MKKVLKVEGMTCGHCKAAVEKALKSVDGVEDAVVSLEEKTAEVT
                     LIKEVPDDVLEKAVTDAGYEVV"
     gene            2330..3607
                     /locus_tag="PLD67_01560"
     CDS             2330..3607
                     /locus_tag="PLD67_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOT21245.1"
                     /translation="MKNNFLSTLFVGIDVSSKTNVLCALDFQGNKLLNLKALNNHPGA
                     ESILESILDCLNSNSLNFVVIALESTSFYSTHIANFLSSNEVLLAYSPMVYCLNPKTI
                     ANYRKSFVDMDKTDPLDAYIIADFARCGRIKSQPWRGAQFLALQRLTRHRLHLVEAIA
                     KEKTYMVSNVYLKFSQLTVLEKADRPFSNTYGATSEAVLTEFLSLEDIAYSSIEDLVA
                     FIKDKGKNRFSNPVGTAELLQKAARDSYRLDKVLYEPINIAIASSFNVIKALQIEIKE
                     IDKAIEKNIKGINTTEYQSLISIPGIGPVFASGILAEIGSITSFSSNDALAKYAGLTW
                     RIKESGDYSASETNMTKTGNKYLRYYLIEAANSVKNNIPEFNVYYRKKFNEVPKHQHK
                     RALALTSRKLVRLIFGLLTKNQIYSSSKVGEIQ"
     gene            3957..5351
                     /locus_tag="PLD67_01565"
                     /pseudo
     CDS             3957..5351
                     /locus_tag="PLD67_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361508.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            5597..6655
                     /gene="ord"
                     /locus_tag="PLD67_01570"
     CDS             5597..6655
                     /gene="ord"
                     /locus_tag="PLD67_01570"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HOT21246.1"
                     /translation="MQNVKVIIWGLGAMGGGMARMLLEKKGVDIVGAVGRGAKLGKSM
                     YEIIGVERGDRPEVLVGTPEDVIKEKAADVVLCCTDSFTKVAFERLKFCLERKINVVS
                     SAEEMAFPQAQNPELAKQLDEIAKANGVSIIGTGINPGLIMDYLVVVMTGCCQTIDHI
                     TARRVNSLSPFGPAVMHEQGIGITVEEFKKGVETGTLSGHVGFHESIGMISEAIGWKV
                     DKVTTSMEPIVTDVDRKSPHGFAKAGDVAGCAMKGYGYVDGELKIEMDHPQQIEPEQV
                     GVQTGDYVIIKGTPNINLVNSPEVPGGIGTIAMCVNTIPMIINAKPGLHTMLTIPAPR
                     AIMGDMRDLIDPECKIVK"
     gene            6739..7044
                     /gene="ortA"
                     /locus_tag="PLD67_01575"
     CDS             6739..7044
                     /gene="ortA"
                     /locus_tag="PLD67_01575"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HOT21247.1"
                     /translation="MVKKGEWVLIHRNVLEPAERVPHVPDDTKKVPLELWTKGYLQED
                     ANIGDEVTVLTRTKRLEKGTLLEVNPCYKHDFGNFVPELLKISEQVKEILFGGADNE"
     gene            7037..>7852
                     /locus_tag="PLD67_01580"
     CDS             7037..>7852
                     /locus_tag="PLD67_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HOT21248.1"
                     /translation="MSKDMSYAAVMARRPEIMKNSAGLDFSKFESGSIAFDYERMMKE
                     AGFTIEEIQKIQSEHGVGNTPIIELRNLTALARKIAPEGKGARIFIKDEAANASGSFK
                     ARRASTAVYQAKKLGYKGVVTATSGNYGAAVASQAAMYGLKCIVVQECYDSRGVGQPE
                     IIEKARKCEALGAEVVQLSVGPELFYTFLTILEETGYFNASLYTPFAVAGVETLGYEI
                     AMQFREKLGKDPDVVMATNAGGGNLTGTARGLKKAGAQSQIVAASVDLSGLHMA"
BASE COUNT         2651 a         1287 c         1455 g         2459 t
ORIGIN      
        1 aagtatgaca gcttgttgag cggctttcat cggcatttct tccgttaacg gttaaggctg
       61 gagctgtgca gcctaatttt ttattaaagt aacaatttgt taccgtacac ttaatttctg
      121 ccattgtctt tctcctttca tcaatgagtt ttctttttag tttatcccaa agatgtttaa
      181 ttatgcctat tgtctgtaat atgccaaaag caaatccacc attcttccgt aacttcttac
      241 tccatcctgc tgattatgcg cctttaaata aatatcattt actttatttg ctgcatctgc
      301 aacctttgtg tcaaactgtt tccaatacct tatcgagaaa tctatatctt ttctaacatc
      361 atccgaatat aaattataca atcttacaaa ttcatcgtaa tcgaccgagt aaagagcatt
      421 catcgaatga gttaaggctg acatggtacc gctgtaatag aaatcatcat atcctgattt
      481 aatgcatgcc aaatatgata tgaagtttgc ttcatcttcc ctcataaatc cccttagatg
      541 caccaattca tgaagcataa caaaaggaat ctgataggga ggtatatgga catttacatt
      601 agcttcaaat gtataaggaa aaaagactcc tgttatctta aagtaggaca taatctttga
      661 ctgtctcaca ggcttcggtg cagaaaaatt tcctttcaat atttcatatt cttccgacag
      721 attctccatg aaatctccgg ctctctctgc tgttgtataa taactcttat ctgataactt
      781 tgcggttcct tcatgggttt cgagctttgc cctgcactca tttgcatctc caatcagcaa
      841 ttcacaaagc tcaatcaatt cctgctttga agacatttgg atttctaagc cgctgtaatg
      901 agtaaattca tacctgtaat agtttattcc gcagaataaa acaaacaaaa agtacatcac
      961 agaaataaat gaagcaaccc cgaaaatata tttttttata tactcaaatg ttctatattt
     1021 tatacttcct attgtgacaa gggctgtaag tattattatt gtcactatta atgcaataat
     1081 tattaattcg gctgctgagt tgcgggtaat aaatgacaat ctgcctacag atttcacaaa
     1141 aaacgggtaa atattaactg catagaattc cgcataattt gcattgtttt ttacaaataa
     1201 gttaatcaat accgaaacag gaatcagcca aagaaaaaac catctttttc tcattttcta
     1261 ccccatccaa aaaatatact agatttttat cataagtgca agccgcaaaa tcggcttgct
     1321 tcacaccgat acaatgaata catcagtgca aagacacagg tctttgcttc attgattgta
     1381 ttcattttat catataatag tactaattac actgattatt tatatcaata aatgtcaaga
     1441 ccaatgaatc tcaattatat ccttcgctta cgctcaggat gacaaatagt acatacctta
     1501 tgcatgaaaa aacccgaggt ttctcgggtt tgcatcagag taagtcccct gccttttaaa
     1561 ctacttcata tcctgcatct gttacagctt tttctaaaac atcatctgga acttccttta
     1621 taagcgttac ttctgccgtc ttttcttcta aagaaactac tgcatcttct acgccgtcca
     1681 ctgatttaag agctttttct acagctgcct tgcaatgtcc gcaggtcatc ccttcaactt
     1741 ttagtacctt tttcatatta ttatctcctc gtataatatg tttagaacta ctttttaagt
     1801 gttctaaaca ttaatttatt tttagcaaat gcaataacta attgaactaa tttatggcta
     1861 tcgattctgt tgatctaact tgtgtactgc taaaattgaa cctaaggttt tcaggcgcat
     1921 attagaatcg taaggaggcg tagccgactg gagattctaa tatgcgcgct ggcttcgccg
     1981 atcatggttc ataaataacg tccaatttat tttgcaattg cgtaaattta ttttgtttca
     2041 ttttcaattt ttaattgaga tattctaatg ctataatgtt gtaaagaaca ttagcgaagg
     2101 ataattcttt cactccttgc aaagccattt ggctattgct taagaagcat gcttcctgac
     2161 ttagttgcca cattctgctt actcagatgc tgcatctctt taattagacc aacagcaagg
     2221 ttagttttta aagatgaatg ctaataatgc gaggtttcag tcagcttcta agattaggat
     2281 atctctttta ctaattgttc ttcaatgtcc tatgaaaggt ggtgatttca tgaaaaacaa
     2341 ttttctatcg actttatttg ttggtattga tgttagttct aaaactaacg ttttgtgtgc
     2401 tcttgacttc caaggcaata agcttcttaa tctgaaagct ctgaataatc accccggtgc
     2461 tgaatctatt ttagaaagca tcttggattg tttaaattct aatagtttaa attttgttgt
     2521 tattgccttg gaatcaactt ctttttacag tactcatatt gctaatttct tgtcttctaa
     2581 tgaagtactt ttagcctatt cacctatggt ttattgtctt aatccaaaaa ccattgctaa
     2641 ttacagaaag tcttttgttg atatggataa aactgatcct cttgatgctt atattatcgc
     2701 cgactttgcc agatgtggaa gaatcaaatc tcaaccatgg cgtggagctc agtttcttgc
     2761 cttgcaaaga cttaccaggc atagacttca tctagttgaa gcaattgcta aagaaaaaac
     2821 atatatggta tctaatgttt atctcaaatt tagccagtta actgttttag aaaaagcgga
     2881 caggccattt tctaatacat atggtgcaac ttctgaagct gttttaactg aatttctatc
     2941 attagaagat attgcttatt cttctattga agatttagtt gctttcataa aagataaagg
     3001 taaaaatcgg ttcagcaacc cagttggaac agctgagctt ttacaaaagg ctgcaaggga
     3061 ttcatatcgt ttagataaag tcctttatga acctataaat attgcaattg cctcttcatt
     3121 taatgttatc aaagctttac aaattgaaat taaagagatt gataaagcaa ttgaaaagaa
     3181 cattaaaggc ataaatacta ctgaatatca gtcattgatt tctattccag gcattggtcc
     3241 tgtatttgcc agtggaattt tagctgaaat tggttctatt acttcctttt cttctaatga
     3301 tgcattagct aaatatgccg gcttaacctg gagaatcaag gagtctggcg attattctgc
     3361 ttctgaaact aatatgacta aaacaggtaa caagtatctt aggtattatt taattgaggc
     3421 tgctaatagt gtgaaaaaca atatacctga gtttaatgtt tattaccgca agaaatttaa
     3481 tgaagtaccc aagcatcaac ataaacgagc actcgcatta acatctcgta aattagtacg
     3541 cctgattttt ggattgctga ccaagaatca aatctactcc agcagtaaag ttggagagat
     3601 acaataaaat atttttctct ctatcctgtt tttttctttg aaattaagag gttctattaa
     3661 agttaccctt ttttaatcat ttcttttgtt tttatttgtt tttttatctt gacatattac
     3721 cagattgctt taatgtttat ctcatataat cctcgattac gcttaggatt acaaatacat
     3781 atgccttaca tcaaatatta atttcatttt ataccgtaat tatacaaaag taaactatca
     3841 tcattgtaaa aattgaattc ttatgataca attaatcatt agaatgggga caggtctata
     3901 ggcgagtcta tatagataag aatagaggaa ggtccccggt aaaattggag gggtctatga
     3961 aagcaaagga atgtttactg ttgctgcaaa aaattttgca gttagtagat gaaggtgttc
     4021 atgtacttga caagcatggc aataccatca tatataatga agccatgtct gctttggaaa
     4081 agatggactc agctgatgta atcaagaaac cttttacaga ggttttcaag ggatttaaca
     4141 ccgaaaacag cactcttcta aaagccttgc attctaaaac cgtaacgaaa aacttaaagc
     4201 aaacatattt gaacaaggac ggaaaagaga tttctacaat taataccaca atacccgtga
     4261 taattgatga tgaggttgtg gctgctgttg aagtagcaaa aaatataact aaaattgaag
     4321 agatgtccaa tacaatatta aaactccatg atgaaataga aaaaccggaa atggttaaac
     4381 ataagaaagt aaagaaatat aattttgaca atattatcgg acacagtgaa aatttcttgg
     4441 atgttattga aagagcgaaa aaatcttcaa aaaattctgc atcagtattt atttacggtg
     4501 aaacaggaac aggtaaggag ttaattgctc aaagcattca ttatgccagc gctagaaagg
     4561 ataagccttt taaagctcaa aattgtgctg ctcttccgga atctttactt gaaggattat
     4621 tatttggcac aacaaagggg gggttcacag gtgcggttga ccgtcccgga ttatttgaac
     4681 aggcaaatgg aagaacgctt ctattagatg aaataaattc aatgccatat gaattgtaaa
     4741 ataagctgtt aagagtactt caagaaaact atataagacg ggttgggggt tcaactgata
     4801 ttcctattga tgtaaggatt attactacat gtaatgagcc tcccgaaaaa atattaaaag
     4861 aaggcaagct aagaaaggat ttattttata ggcttaatgt aattccaata aatgtacctc
     4921 ctttaagaga aagagttgat gatattattt tgcttgctaa aatgtttgtt gataaacaca
     4981 atgagaggtt taacaaacac gttacaggtt tttcggatga agtatatgaa tatctcttat
     5041 catatgatta tccgggcaat gcaagagaac ttgaaaatat gattatgtca gctgtcagca
     5101 tgtgtgaaga tgatgaatat attttgactg ttaatcattt ttccgtgcat cgttcaattg
     5161 agcctcgaaa tgacgattat gaaaaagtag aagattccgg cattgatgaa tatttaggaa
     5221 atttagaaaa aatgataatt gagaaagcac ttttagaaaa taacaataac attaccagaa
     5281 catcaaatgc attaaaaata aagcgtcaga ccttacagca taaaataaag aaatataaaa
     5341 ttagtgttta aaagcaaata tttttgcacc tagctgcaaa aatacttgct ttttttatat
     5401 tatttctgaa ttgtttgtta tataaagagg gaatgtactg gaataagttg cttattttga
     5461 gataaaataa cttggagttt gaaatattaa agttaaatat caaataacta ataaatttat
     5521 ttattttaat gtttttggca taataattgc ttattaaata ataaatagag tagaaaaata
     5581 ttaggaggat taacaaatgc aaaatgtaaa agtaattatt tggggactcg gagccatggg
     5641 aggcggcatg gcaagaatgc ttctggaaaa gaaaggtgtt gacatagttg gtgcagtcgg
     5701 cagaggtgcc aaattaggaa aaagcatgta cgaaattata ggagtagaaa gaggagacag
     5761 acctgaagta ctcgttggaa ctcctgaaga tgttattaaa gaaaaagctg cagatgtagt
     5821 tctatgctgt acggattcat ttactaaagt agcatttgaa agactaaaat tctgcttgga
     5881 aagaaaaata aacgtagtat caagtgcgga agaaatggca ttcccgcagg ctcagaatcc
     5941 tgaacttgca aaacaattgg atgaaatagc taaagctaac ggtgtttcaa taattggaac
     6001 cggtattaac cccggattaa ttatggacta cttggttgtt gttatgacag gatgctgcca
     6061 aacaattgac cacattacag caagaagagt taacagctta tcgcccttcg gacctgcagt
     6121 tatgcatgag caaggtatag gcatcactgt tgaagaattt aaaaaaggtg ttgaaacagg
     6181 aacactatca ggtcacgtcg gattccatga atcaatcggt atgatttcag aagctatagg
     6241 ctggaaagtt gataaagtaa ctacctcaat ggagcccatt gttacagacg tagataggaa
     6301 atctcctcat ggatttgcta aagcagggga tgttgcaggc tgcgcaatga agggttatgg
     6361 atatgttgac ggcgaattga aaattgaaat ggaccatcca cagcaaatag agcctgaaca
     6421 ggttggtgtt caaacaggtg actacgtaat aatcaaagga actccaaata ttaatttggt
     6481 taactctcct gaagttccgg gaggaatcgg tactattgct atgtgcgtaa atactattcc
     6541 gatgattata aatgctaaac cgggattaca tacaatgctt actatcccgg caccaagagc
     6601 aatcatggga gatatgagag atttaatcga cccggaatgc aaaatagtaa aataaagatg
     6661 acctaatcct attttttcaa gctaaggcga agaaaaaaca aaagcgaaat taataaaata
     6721 aaggtaagga gcgtaataat ggttaaaaaa ggtgaatggg ttttaataca cagaaatgtt
     6781 ttagagccgg ctgaaagagt tccacatgtt cctgatgata ctaaaaaggt tcctttggag
     6841 ttatggacaa aaggatactt acaggaagat gcaaatatcg gagatgaagt aacagtatta
     6901 acaagaacaa aaaggttgga aaaaggcact ttgctggaag taaatccatg ttacaagcat
     6961 gatttcggca actttgttcc tgaattattg aaaatcagcg aacaagtaaa agaaatttta
     7021 tttggaggtg ctgataatga gtaaagatat gagttatgct gccgttatgg caagaagacc
     7081 tgaaataatg aaaaattcgg ccggtttaga tttttctaaa tttgaaagcg gttcaatagc
     7141 ttttgattat gaaagaatga tgaaagaagc cggatttact attgaagaaa tccaaaaaat
     7201 tcagtctgaa cacggagttg gcaatactcc aattatagag cttagaaatt taacagcatt
     7261 agcaagaaaa atagctccgg aaggaaaagg agcaaggata ttcataaaag atgaagcagc
     7321 aaatgcatcg ggaagcttta aagcaagaag agcatcaact gcagtatatc aagctaaaaa
     7381 gcttggatac aaaggtgttg ttacggctac aagcggcaat tacggcgctg cagttgcatc
     7441 acaggcagca atgtatgggc ttaaatgcat tgtagttcag gaatgctatg attcaagggg
     7501 tgtaggtcag cctgaaataa tcgaaaaagc aaggaagtgt gaggctttgg gggctgaagt
     7561 tgttcaactg tcggtaggcc ccgagttatt ctacacattc ctgacaatat tggaagagac
     7621 cggatacttc aatgcatcct tgtatacacc gtttgcagta gccggtgtag aaacattggg
     7681 ttatgaaatt gcaatgcagt tcagagaaaa gcttggcaaa gacccggatg ttgtaatggc
     7741 aacaaatgca ggaggcggaa atcttacggg aactgcaaga ggacttaaaa aggcaggggc
     7801 tcagtctcaa atagtggctg ccagcgtaga tttgtcggga cttcatatgg ca
//
LOCUS       DAOSOW010000031         7797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_9325_length_7797_cov_4.182640, whole genome shotgun sequence.
ACCESSION   DAOSOW010000031 DAOSOW010000000
VERSION     DAOSOW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9325_length_7797_cov_4.182640"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PLD67_01585"
     CDS             complement(<1..670)
                     /locus_tag="PLD67_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21249.1"
                     /translation="MDWNKTNTILITAFIILNVFLFVSSKNIRNAEHKPNYDKEFAQE
                     VAYLLEEKNIKINVEIPNNIYILPVLETEYEIIKIDSNLLTNFLNERIEPFEDVYKYT
                     NSKGETLEIIDGKKLRLTLRNKVDDKINEDSKIQEEINNFLKEKNIDASGYVESFKHT
                     SENECIYAYTLKYNDYSIDNSYMYFFVDNEGIYKFEMQRIISVDEIPEKIRTIYAIEA
                     LPRIL"
     gene            complement(683..1927)
                     /locus_tag="PLD67_01590"
     CDS             complement(683..1927)
                     /locus_tag="PLD67_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21250.1"
                     /translation="MRPVKNVIKYDDNYTVLYSDNKGLWQEALNVIYDAFNATNSTIS
                     ETAAFPAEYLKFDFNINIPVEIFTTHMKTGNNNINNTVKNIKNVTIDLKDNNSIYIYN
                     GENNIKIKSNQINSKKLTDIVLSINFENKTKYSMLEKIGDETIQVPVPLEKTALNPVF
                     VQSELDVFDIDRINEIAKDYFKNDYDYVRKSVEVSGNLVYVYRTEKILKINEDGLLDF
                     YDASIEPENSPNVYKSFAAAVNFIEEFLGFPENGYLSNVEKILLEGNEGYRFTFSYNI
                     LERPILFSRVRENAALQIDVAGNNVISYKRFIRNIDRTQMDKMAEIEVTPAIEVIRNN
                     IDTSAGEENADTVPGEGQEEAVQLKPIKPEMIKEINNIYLGYFDLSRISREQVLRVVW
                     VIETNEKTFIFNAISGLLIEEW"
     gene            complement(2097..3878)
                     /locus_tag="PLD67_01595"
     CDS             complement(2097..3878)
                     /locus_tag="PLD67_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT21251.1"
                     /translation="MFKSIRWRFILIYFLLVFLAMSIVGIYIVNQLEGIQLNMNIKNM
                     DARIRSIIQSYSSLNTGNWDENIEEIQKSISTVQVGYNENIYVILNDVNRTIIAGSVE
                     ESIGISAFNYNKIDNYILTKSVDGTPYHIAPAEQFEDTENQRFYYHMSFPVQTEGNIK
                     GYIYLVNNIDYIYDTVDESKQIMTQATIIALTLTIFLGLILSTSITGPIKELTIKARQ
                     MSLGDFEQKVNVKSDDEIGQLGNMFNYLIDQLKKSMSSLQQEKSKIETTFTYMADGVL
                     TVDLLGNIIHANPVAKNILSLKNADEKFDDVIVNLKDSISIDYIKSKGYRGTDLAEKN
                     GETYNIDYAPFMNNKNEIGGVILVFKNITEQHRIDKLQKEFVANVSHELKTPITTIKS
                     YAETLLDGALEDKQISEEFLNVINSESDRMTRLVSDLLRLSKMDYEQTNWNKERINLL
                     DMVNIVIKKLQIQIKEKNINIHLNIPDDINIMFDRDGFEQILLNIAGNAVKYSYEKGN
                     VWISAKKSNKNIVVRIKDDGQGIPIEDQDRIFERFYRVDKARARDLGGTGLGLSIAKE
                     IAEAHNCTIAVNSRIDEGTEMIITIPE"
     gene            complement(3913..4596)
                     /locus_tag="PLD67_01600"
     CDS             complement(3913..4596)
                     /locus_tag="PLD67_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT21252.1"
                     /translation="MPKILVVDDERPIAEIIKYNLQKEGFEVQTAYDGDEAIKAVHNF
                     QPELIILDIMLPKKSGFDVLKEIRMEYVMPVIMLTAKDEENDKVTGLELGADDYITKP
                     FSNKELIARVKANLRRVKLSKVEEEMKSKIIAVGNLTIDMNTYEVFKDGEIIDLTNRE
                     FDLLKYLFQNSDRVFNREHLLKEVWGYEFGDLRTVDVTVRRLREKIETEDEKYIITKR
                     GTGYYFKSK"
     gene            4824..5996
                     /locus_tag="PLD67_01605"
     CDS             4824..5996
                     /locus_tag="PLD67_01605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOT21253.1"
                     /translation="MRNYIDLNKVCPVIKGRLSSSSNNIIPVIISYKSGKKVKEGQIS
                     SLSNRLRHNLPIINGCACEMSTESILKLTAEPDVEFISYDSKVFAVMEEARKTIGADY
                     SLNTKYTGKDVTVAIIDTGVAPHADLLYPENRIVGFKDFVNNRSKYYDDNGHGTHCAG
                     ILAGSGYSSKGKYRGIAPEANILSIKVLDENGNGNTSDILSTVQWIIETKEVYKTKII
                     NFSLGAIAQYKERRDPLVKAANKAIEENLIVVAAVGNSGPMHNTILSPATSRYVISVG
                     SLNDRGTSHFENETIAEFSSRGPTLDRVRKPDLIAPGVDIISLSNKNLSGYTALSGTS
                     MSAPMVSGAAALLLNKNPNYTHFDIKRILLNSCSKIKASSYEQGAGVLDMRRIFSQ"
     gene            complement(6018..6905)
                     /locus_tag="PLD67_01610"
     CDS             complement(6018..6905)
                     /locus_tag="PLD67_01610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT21254.1"
                     /translation="MKEFADYISDAFVHCRMTYNDGKFDIEALYANKQLEHITNKTMD
                     QLIGKKMTEVFPNLADSIFDWPKILCEAAMTSEHSVIEQYVNAFDKFVKFNIFGFKDD
                     TFYMTIQDLTHKREIRRLILEKDRQIEHLENEIKSRASIEPLTNIYNFQFITDCMKHS
                     IQSYKEEGTAFCVFLIDIDDFKEFNRKYGIETGDMVLQDVALILTLAARKIDVVGRYG
                     NDKFIMILNNVELDIAKIIVEKVKMEIGRYDLNISNNTIHASGAVVEYRGETLEEFVE
                     KAEELLKKAQAMGKGKILS"
     gene            complement(6920..7561)
                     /locus_tag="PLD67_01615"
     CDS             complement(6920..7561)
                     /locus_tag="PLD67_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HOT21255.1"
                     /translation="MKKITKIEYQKKNKERFNIYIDDEYGFAVDISILIKYSLKKGME
                     LDDALIDEILLSEEEISVYNYGISVLSRYFKSEYELRLKMKNKGFNPQLIDNAISILK
                     EHKYLDDERYCEMFINDKINISKHGVRKIKEALYYKGIDKEIIEEKIKCISAESEEER
                     ALLLGEKKLLNIKENDNRKKMSKLSNYLLGKGFEYETVNRALRKLLNADNDFY"
BASE COUNT         2278 a         1487 c         1104 g         2928 t
ORIGIN      
        1 ttaaaattcg cggtaatgct tcaatagcat atattgtacg aatcttttcc ggtatttcat
       61 caactgaaat tattctctgc atttcaaatt tataaattcc ttcattatct acaaaaaaat
      121 acatatagga attgtctatt gaatagtcat tatattttag cgtatatgca taaatacatt
      181 cattttcgga agtgtgtttg aaactttcaa catatccgga tgcatcaata tttttttctt
      241 ttaaaaaatt atttatttct tcttgaattt ttgaatcttc gtttatttta tcgtcaacct
      301 tattgcgcaa tgtgagtctt aatttcttgc catcaattat ttctaatgtt tcgcctttgc
      361 tgttcgtgta tttgtacaca tcttcaaaag gctcaattct ttcatttaag aaatttgtca
      421 aaagatttga atctatctta attatttcat attctgtttc caaaacaggc agtatatata
      481 tattattcgg tatttcaaca ttaattttta tatttttttc ttctaataaa tatgctacct
      541 cttgggcaaa ttccttatca taattaggct tgtgctctgc atttctaata ttcttggaag
      601 acacaaaaag aaaaacattt aaaattataa aagctgttat taatattgtg tttgttttat
      661 tccaatccat ttgcctcacc tgttaccatt cttcaattaa aagacctgat atggcattaa
      721 atataaatgt tttttcattt gtttcaataa cccataccac ccttaaaacc tgttcccttg
      781 aaattcgaga taaatcaaaa tatccaagat aaatattgtt aatctcctta atcatttccg
      841 gctttatggg tttaagctgt acagcttctt cctggccttc cccgggtact gtatctgcat
      901 tttcttcacc ggccgaagtg tcgatattgt ttcttatcac ttcaatagca ggagtaacct
      961 ctatctcagc cattttgtcc atctgtgttc tatcaatatt tctgataaac cttttgtatg
     1021 aaataacatt gtttcctgca acatcaattt gaagagcagc attttctctt accctgctga
     1081 acaatatggg cctttccaat atattgtagg agaatgtaaa cctataacct tcatttcctt
     1141 ccaagagaat cttttctaca ttgctcaaat acccgttttc cggaaacccc agaaattctt
     1201 cgataaaatt tacggctgca gcaaagcttt tataaacgtt cggactgttt tccggctcga
     1261 ttgatgcatc gtagaagtct aagagtccat cctcgttaat ttttaatatc ttttcggtcc
     1321 tgtaaacata taccaaattc ccgcttactt caacagattt tctaacatag tcgtaatcat
     1381 ttttaaaata atctttggca atttcattaa ttctgtcaat atcaaataca tcgagctccg
     1441 actgaacaaa tacagggttc agtgctgtct tctctaaggg aacgggtacc tggattgttt
     1501 catcccctat tttttccagc atggaatatt ttgttttatt ttcaaaattt atagatagca
     1561 caatatctgt taatttcttg gaattaattt gattactctt aatttttata ttgttttcgc
     1621 cattgtaaat ataaattgaa ttgttatctt tcaaatctat cgtaacattc tttatatttt
     1681 ttacagtatt gtttatatta ttgtttccgg ttttcatatg ggttgtaaat atttctacag
     1741 gaatattaat attaaaatca aatttcaaat attcagccgg aaatgccgct gtttccgaaa
     1801 tggtgctatt tgtagcatta aaagcatcat atattacatt taatgcttcc tgccacaacc
     1861 ctttattatc tgaatacaaa acagtatagt tatcatcata tttaattaca ttttttacag
     1921 gtctcaatac tttccatatg tctgcttcgg cttcttccgc acttttatct tcttatgcct
     1981 tagagcttaa aaattcagga agttttagcc atacattgct gcttaaatat atacatatga
     2041 gtaccatgac caataatatt gaatttttta tttttatatt taacatagtg tcctctttat
     2101 tcaggaattg taataatcat ttcagttcct tcatctatcc ggctgttgac ggctattgta
     2161 cagttgtggg cttctgcaat ctctttggca attgaaagtc caagaccggt tcctcctaaa
     2221 tccctggctc ttgctttgtc cacacgatag aatctttcaa aaattctatc ctggtcttct
     2281 atcggaatac cctgcccgtc atctttgatt ctaacgacta tatttttatt gctcttttta
     2341 gcgctaatcc atacatttcc cttttcatat gaatacttaa ccgcatttcc tgcaatattc
     2401 aaaaggattt gctcaaatcc gtccctgtca aacattatat ttatatcatc cggaatattt
     2461 aaatgaatat ttatattttt ttcttttatt tgaatttgca gttttttgat gactatatta
     2521 accatgtcca gcaaattgat tctttcttta ttccaatttg tctgctcata atccatcttt
     2581 gaaaggcgca ataaatcgct gacaagcctt gtcatcctat cgctttcgga atttatgaca
     2641 ttcagaaatt cttcggatat ttgtttatct tccaatgcac cgtccaatag agtttctgca
     2701 taacttttta tggtagtgat cggcgttttt aattcatgcg atacatttgc gacaaattct
     2761 ttttgaagct tatctattcg atgctgctcg gtaatatttt taaacacaag aatcacgccc
     2821 cctatttcat ttttgttgtt catgaaaggt gcgtagtcta tattataagt ttctccgttt
     2881 ttttctgcca agtctgtgcc gcgataacct ttagacttaa tatagtcaat tgatatgctg
     2941 tctttgagat ttactattac atcatcaaat ttctcatcgg cattttttag tgataatata
     3001 ttttttgcaa cggggtttgc gtgtataata ttacctaata aatcaacggt caaaactcca
     3061 tctgccatat atgtgaaggt ggtttctatc ttgctttttt cctgctgaag gcttgacatt
     3121 gactttttca gctggtctat aaggtaattg aacatattgc ccaattgacc tatttcatca
     3181 tctgatttta catttacctt ctgctcaaaa tcgcctaatg acatctgcct tgcctttata
     3241 gtgagctcct tgattggtcc cgttatgctt gttgaaagta tcagtcctaa aaatattgtt
     3301 aaggttaaag caattatggt agcttgtgtc ataatttgtt ttgattcgtc aaccgtgtcg
     3361 taaatataat caatattatt tactaaatat atataacctt tgatgtttcc ttccgtctgc
     3421 accggaaaag acatgtgata ataaaacctt tgattttcgg tatcttcaaa ttgttctgcc
     3481 ggtgcaatgt gataaggagt tccatccact gatttagtca gtatataatt gtctatttta
     3541 ttatagttga aggcacttat gccaatcgat tcttctacac tgcctgcaat aattgttctg
     3601 tttacatcat ttaagattac atatatattt tcgttgtacc ctacctgtac agtgcttatg
     3661 cttttttgaa tttcttcaat attctcatcc cagttaccgg tatttaatga gctgtaggat
     3721 tgaataattg agcgaattcg agcatccatg ttttttatat tcatgttaag ctgaattccc
     3781 tcaagctgat tgactatata gatgccgact attgacatag caagaaaaac caacaaaaaa
     3841 taaattaaaa taaatctcca tcttatgctc ttgaacattt tttctcctta gaatacgcag
     3901 caaaatttgg catcatttgc ttttaaaata atatcctgtc cctcgttttg tgattatata
     3961 tttttcatct tctgtttcta ttttttctct taatcttctc actgtcacat caactgttct
     4021 taaatcacca aactcatatc cccatacttc cttaagaagg tgctctctgt taaatactct
     4081 gtctgaattt tgaaacaaat attttaaaag gtcaaattcc ctgtttgtaa gatctattat
     4141 ttcaccatct ttgaaaactt catatgtgtt catgtcaatg gttagatttc ctactgctat
     4201 tatctttgat ttcatttctt cttcaacctt tgaaagcttc acccttctca aattagcctt
     4261 gactcttgca attaattcct tgttgctgaa aggcttggtt atatagtcgt ctgctcccaa
     4321 ttcaagccct gtaaccttgt cgttttcttc atcttttgca gtaagcataa taacaggcat
     4381 tacgtattcc atgcgtattt cttttaaaac atcaaatccg cttttctttg gcagcataat
     4441 atcaagtatt attaattcag gctggaaatt gtgcactgcc tttatagctt catccccgtc
     4501 ataggctgtc tgcacttcaa agccttcttt ttgaagattg tatttaatta tttcggctat
     4561 aggtctttca tcatccacta ctaatatttt aggcattatt tcacccctat tttaattact
     4621 gtctttaatc atcagaagaa attgccgtgc aatttcaccg tttcaacgag gcgggatata
     4681 cccgcctgaa tttcacatat atccgcctcc taaggaggaa gatatctttc ccattatatt
     4741 attcatgaat attataacat gtttttcaaa aatgaacacc atctttattt aaaagcataa
     4801 attaatttag gtaggtgaat tttatgagaa attatataga tttaaataaa gtgtgtccgg
     4861 ttataaaagg acgcctttct tcaagcagca ataatattat acctgttatc ataagttata
     4921 aatccggcaa aaaagtaaag gaggggcaaa tcagttcgct atcaaacaga ttaaggcaca
     4981 atctgcccat tatcaacggt tgtgcttgtg aaatgagcac tgaatcaatt cttaaattaa
     5041 cagctgagcc cgatgttgaa tttatatcct atgattcaaa agtatttgcg gtaatggagg
     5101 aggcaagaaa aacaatcgga gctgactatt ccctaaatac taaatatacg ggaaaagatg
     5161 taactgttgc aataattgat acaggtgtcg cccctcacgc tgatttgtta tatcccgaaa
     5221 acaggatagt tggctttaag gattttgtaa acaatcgctc caagtattac gatgataacg
     5281 gtcatggaac acattgcgca ggaatacttg caggaagcgg ttattcttcc aagggcaaat
     5341 atagaggaat agccccggaa gccaatatac tatcaatcaa ggttcttgat gaaaacggaa
     5401 acggaaatac atcggatatt ctatctacag ttcaatggat tattgaaacc aaggaggttt
     5461 ataaaactaa aataatcaat ttttctctcg gcgcaatcgc tcagtataag gaaaggagag
     5521 atccattggt aaaggcggca aacaaagcaa ttgaagaaaa tctcatagtg gttgctgccg
     5581 ttggcaacag cgggcctatg cacaatacaa tattatctcc tgcaaccagc aggtatgtaa
     5641 tttccgtagg ctcattaaat gacaggggca cgtctcattt tgaaaatgag acaattgctg
     5701 aattttcaag cagaggccct acattggaca gagtccgcaa accggattta atagctcccg
     5761 gagttgatat aatatcttta tccaataaaa acttaagcgg ctacaccgca ttgagcggta
     5821 catcaatgtc cgccccaatg gtatccggag ctgccgcttt acttttaaac aaaaatccca
     5881 attacaccca tttcgacatt aagagaatac tccttaattc atgctcaaaa ataaaagcat
     5941 cttcatatga gcaaggagca ggagttctag atatgagacg cattttttca cagtaataaa
     6001 gggagcgcaa tgctccctta tgacaatatt tttccctttc ccatagcttg tgctttttta
     6061 agtaattctt cagctttttc aacaaattct tccaatgttt cgcccctgta ttccacaacg
     6121 gctccgcttg catgaatagt attattgctt atattcaaat catatctgcc gatttccatt
     6181 tttacttttt caacaattat ttttgcaata tcaagttcta cattgttcaa tatcataata
     6241 aatttatcat ttccatacct gcccaccaca tctatttttc ttgcagccaa agtgagtatg
     6301 agggctacat cttgtaaaac catatctcct gtttcaatgc cgtatttacg attaaactct
     6361 ttaaagtcat caatatctat caagaatacg caaaatgccg ttccttcttc cttatagctt
     6421 tgtatactgt gtttcataca gtcggtaata aattgaaagt tatatatatt ggtaaggggc
     6481 tcaatgcttg ctcttgattt gatttcattt tccaaatgtt cgatttgtct gtctttttca
     6541 agtattaatc tcctgatttc ccttttatga gttaaatcct gaattgtcat ataaaaagta
     6601 tcatctttaa atccaaatat attgaatttt acgaacttat caaaggcatt gacgtattgt
     6661 tcaattaccg agtgttcact tgtcattgca gcttcgcata aaattttcgg ccagtcaaat
     6721 attgaatcag caaggttagg aaatacttcc gtcatttttt tcccgattaa ctggtccatt
     6781 gttttattgg taatatgctc taactgctta tttgcataga gagcttcaat atcaaatttt
     6841 ccatcattat aggtcatcct gcaatgaaca aatgcatcgg atatataatc tgcaaattcc
     6901 ttcataagta tagccccctt tagtaaaaat cattatctgc atttaataac tttcttagtg
     6961 ccctatttac cgtttcatat tcaaagccct ttcctaacaa ataattcgac aactttgaca
     7021 tttttttgcg gttatcattt tcttttatgt tcaaaagttt tttctctccc aataataaag
     7081 ctctttcttc ttcgctctct gctgaaatac actttatctt ttcttctatt atttctttgt
     7141 caatgccctt gtaatataaa gcttccttaa ttttacgcac accatgtttg gaaatgttga
     7201 ttttatcgtt tatgaacatt tcacaatatc tttcatcatc taagtattta tgctctttaa
     7261 gtatgctgat ggcattgtcg attaactgag ggttaaagcc tttatttttc attttaagcc
     7321 ttaattcata ttcgctttta aaatatcttg aaagcacaga aattccgtaa ttatatacac
     7381 ttatttcttc ttctgataac aatatttcat caatgagagc atcgtctaat tccatacctt
     7441 ttttgagcga atatttaatc aatatgctta tatcaacagc aaacccatat tcatcatcta
     7501 tatatatatt gaatctttct ttatttttct tctggtattc aattttggta atctttttca
     7561 tatttccctc attttctaca tattaacaca gattttgaag aatatcaata tttataaagc
     7621 aatattatat gggcatatct aagataaaaa agtatccgaa ataataccca aaaaaattgt
     7681 cctcttaaaa ggacaatttt tcggataaat tatcttgcct gttatggcac ctttatgtca
     7741 tgctttggtc gtttttagta aatgttgcac aatccgtttc atgtgatgaa gttgcat
//
LOCUS       DAOSOW010000032         7759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_9389_length_7759_cov_4.050623, whole genome shotgun sequence.
ACCESSION   DAOSOW010000032 DAOSOW010000000
VERSION     DAOSOW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9389_length_7759_cov_4.050623"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..106)
                     /locus_tag="PLD67_01620"
     CDS             complement(<1..106)
                     /locus_tag="PLD67_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21256.1"
                     /translation="MKMGFELNLSQTQKLIMTPELRQAIQILQFNNVEL"
     gene            complement(271..1446)
                     /gene="grdD"
                     /locus_tag="PLD67_01625"
     CDS             complement(271..1446)
                     /gene="grdD"
                     /locus_tag="PLD67_01625"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007157941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HOT21257.1"
                     /translation="MSEKLVKQTIGNIFNQLADTLISGEYGQKPKVAVTALGSEHGED
                     EVYKAGINSLKFGIDTTVIGSKPNESVNTEVVNSEDDAHKKMEELLDKGSVDAAVTMH
                     YPFPIGVSTVGRIITPGKGKEMFIATTTGTSSTDKVEGMVKNAVYGIITAKACGIKNP
                     TVGILNVDGARQTEIALNKLKNKGYEINFAESKRSDGGAVMRGNDLLMGSCDVMVMDS
                     LTGNLMIKIFSSYTTGGSYESLGYGYGPGIGENFNKLILIISRASGAPLIEGAIRFAG
                     ELIKGNCLQIAKEEFNNAKKAGLDEILSEIKASKKSTAEEAEIVAPVKEVVTSQISGI
                     EIMDLEDAVKLLWKNGIYAESGMGCTGPIILVNEAKTNDALKILAANDFIAKEQIDC"
     gene            complement(1451..2989)
                     /gene="grdC"
                     /locus_tag="PLD67_01630"
     CDS             complement(1451..2989)
                     /gene="grdC"
                     /locus_tag="PLD67_01630"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HOT21258.1"
                     /translation="MNSVILGSSYIMVFAPDMVINNGTTQTTERTVNPNSEYLKEIRN
                     HLRTYEQVVSYLPNQTYIGNITPDELSKYEMPWFDKPLEGATREGKFGEIMPQDEFIV
                     LMQICDVFDLVKLEKQFVSAVKAKLMNHPLIDDSLIGRIKEGEEFDTIKKYVEAEHAE
                     PIYNNGALVGCVKRAHDIDINLSGHVMFENLVSKASNVLALLHLISKNNINKEEIEYI
                     IECSEEACGDMNQRGGGNFAKSCAEVAGLVNATGCDTRGFCAAPTHAIVEAAALVKAG
                     VFKKVIVSAGGSTAKLGMNGKDHIKKGYPILEDTIGGFAVLIGENDGVNPEINLDLVG
                     RHTVGTGSSPQAVITSLVSSLDKAGMKITDVDKYSVEMQNPEVTKPAGAGDVPLANYK
                     MIGALAVKRGDIDRKELENFTAKHGMTGWAPTQGHIPSGVPYLGFCREDILSGKIKNA
                     MIVGKGSLFLGRMTNLFDGVSFIIEANKGGQKETSAISEEQIKNLIAKALKDFASNLL
                     NEQE"
     gene            complement(3139..3612)
                     /gene="grdA"
                     /locus_tag="PLD67_01635"
     CDS             complement(3139..3612)
                     /gene="grdA"
                     /locus_tag="PLD67_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_145084612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(3481..3483),aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HOT21259.1"
                     /translation="MSLLENKKIIIIGDRDGIPGQAIEECVKTAGGEIVFSSTECFVU
                     TSAGAMDLENQKRVKEFAEKYGPENIVVILGAAEGEAAGLAAETVTNGDPTFAGPLTG
                     VQLGLSVFHICEPEIKELVDESVYDEQVGMMEMVLDVDDIISEMTAIREEFSKYL"
     gene            complement(3640..3957)
                     /locus_tag="PLD67_01640"
     CDS             complement(3640..3957)
                     /locus_tag="PLD67_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOT21260.1"
                     /translation="MLVLDKTNFETEVLQGEGYIFVDFFGDGCAPCEALMPHVEKLAE
                     KYGDKLKFTKLNTTKARRVAIGQKVLGLPVIAIYKDGEKIEELVKDDATPENIEAMIQ
                     KYI"
     gene            complement(3968..4903)
                     /gene="trxB"
                     /locus_tag="PLD67_01645"
     CDS             complement(3968..4903)
                     /gene="trxB"
                     /locus_tag="PLD67_01645"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOT21261.1"
                     /translation="MSKVYDVLIIGAGPAGLSAGIYAGRARLSTLIIEKEKDGGQIVL
                     TSEVENYPGCLEGESGPTLVDRMAEQAKHFGVEKVYDEIVEVKLEGKEKILIGKKGEY
                     KGKAVIIASGATSRPIGCPGEKTYTGKGVSYCATCDAAFFEDLEVFVVGGGDTAVEEA
                     LYLTKFARKVSIIHRRNELRAAKSIREKAEANPKIHFIWDSVVKEIKGDGIVNSIVLE
                     NVKTKELTEIVADEDDMTFGIFVFIGYEPKTKIFEGILDIENGYLVTDDNMKTNIEGV
                     FAAGDVRVKSLRQVVTAAADGAIAAVQAEKYIETL"
     gene            complement(5226..6530)
                     /gene="grdB"
                     /locus_tag="PLD67_01650"
     CDS             complement(5226..6530)
                     /gene="grdB"
                     /locus_tag="PLD67_01650"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5487..5489),aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HOT21262.1"
                     /translation="MLKVVHYINQFFANVGGEEMAHIPAEVREGVVGPGLAFQQAFKD
                     EAEIVATIICGDSYFNENMDEAKNTIIEMVKKYNPDLFIAGPAFNAGRYGVACGAISK
                     TVKDELNIPVITGMYVENPGADMFKNDIYIVSTKNSAAGMRDAVKKMAPLGLKLAKGE
                     KIGASIEEGYIPSGKRVNFFEKERGSTRAVKMLLAKLNNKEFETEFPMPDFDRVPPNP
                     AIKDITKAKIALVTSGGIVPKGNPDHIESSSASKYGKYDIEGVMDLTEETYQTAHGGY
                     DPVYANLDPDRVLPVDIMREFEKEGKIGSLHRYFYTTVGNGTAVKSAKGFAEKYVKEL
                     IADGVDAVILTSTUGTCTRCGATMVKEIERAGIPVVHMCTIVPISLTVGANRIVPTIA
                     IPHPLGNPSLSIEEEKEIRRKLLTRALKALETEVTEQTVFEK"
     gene            complement(6547..>7759)
                     /locus_tag="PLD67_01655"
     CDS             complement(6547..>7759)
                     /locus_tag="PLD67_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HOT21263.1"
                     /translation="GALYVNKEEVKALILEDQNFKTADVELAKPGESVRIMPVKDVIE
                     PRVKVEGPGGIFPGMVSKVDTVGSGKTNVLKGAAVVTTGKIVGFQEGIIDMSGTGAEY
                     TPFSKLNNLVIICEPIDGLKQHEHEKALRFAGYKVALYLGALAKDLTPDEVEVFETPN
                     LVEGIKMYPELPRVAYVFMLQSQGLLHDTYVYGVDAKQTLTTMIYPTEVMDGAIVSGN
                     CVSACDKNTTYHHLNNPIIYDLFKEHGKTLNFVGVIITNENVYLADKERSSNWSAKLA
                     GFLGVDGVIVSEEGFGNPDTDLIMNCKKIEAKGIKTVLVTDEYAGRDGTSQSLADADV
                     SANAVVSAGNANEVIELPPMEKIIGHVEVADVIAGGFDGSLHKDGSITAELQVITGAT
                     NEMGFNKLSAR"
BASE COUNT         2097 a         1734 c         1148 g         2780 t
ORIGIN      
        1 ttaattctac attattaaat tgcaagattt gaatggcttg ccttaattcc ggtgtcatta
       61 taagcttttg agtttgggaa agatttaatt caaatcccat tttcatggta tcactcctca
      121 caattgtatt atatcaaaaa ttcataaatt ttcaatataa ttataaagca ataaaaattc
      181 aatctaataa acataccaac ataaaaaatt cttcatcctt tgatgaagaa tttttgtggg
      241 gtttgcacct aaaatagggt tagggcatct ttaacagtct atctgttctt tagctatgaa
      301 atcattagct gcaagtattt ttagagcatc atttgtttta gcttcattta ccaaaattat
      361 cggtcctgtg catcccattc cgctttcagc ataaataccg tttttccaca acaatttaac
      421 cgcatcttca aggtccataa tttcaattcc ggatatttgg gaagttacaa cttcttttac
      481 aggagcaaca atttctgctt cttccgcggt tgacttttta gatgctttta tttctgataa
      541 tatttcgtcc aaacctgctt ttttggcatt gttgaattct tcttttgcta tctgcaggca
      601 gttgcctttt atcagttcac ctgcaaacct tatggctcct tcaattaatg gagcaccgga
      661 agccctggag ataataagta tcaatttatt gaagttttct ccaataccgg gaccatatcc
      721 ataacccaag gattcataac ttcctcccgt agtataggaa gaaaatattt taatcatcag
      781 attgcctgta agagaatcca taaccataac atcacaagaa cccataagca agtcattgcc
      841 tcgcataaca gctcccccgt ctgatctttt ggattcagca aagtttattt catagccctt
      901 atttttaagc ttgtttagtg ctatttccgt ttgtctggca ccatctacgt ttaaaattcc
      961 aaccgtcggg ttttttattc cgcaagcttt agctgtaatt attccataga cagcattctt
     1021 aaccattcct tcaaccttgt ctgttgaaga agttccggtt gtcgtagcaa taaacatttc
     1081 ttttccttta ccgggagtta tgattcttcc aacggtggat actcctattg gaaacggata
     1141 atgcattgta acggctgcat caacactgcc cttatcaagc aattcttcca tttttttatg
     1201 agcatcgtct tccgaattta caacttctgt attgacactt tcattaggct ttgagccgat
     1261 tactgttgta tcaattccaa actttaatga attaatacca gctttgtaaa cttcatcttc
     1321 tccatgctca cttcccaatg cggtaaccgc aacctttggc ttttgaccat actcaccgct
     1381 tattaaagta tcggctaatt ggttaaaaat attacctatt gtttgtttta ctaatttttc
     1441 agacatatct tcactcctgt tcgtttaaca gatttgaagc aaaatccttt aatgccttgg
     1501 caattaaatt ctttatctgt tcctctgaaa ttgcggacgt ttctttctga cctcctttat
     1561 ttgcttcaat aataaaggat acaccgtcaa ataaattagt cattcttcca aggaataagc
     1621 ttcccttacc aactatcatg gcgtttttaa ttttgccgga aagtatatct tctctgcaaa
     1681 atccaaggta cggaacacct gaaggtatgt gaccctgagt aggagcccag cctgtcatac
     1741 catgtttagc cgtaaaattc tccaattcct ttctgtctat atccccgcgt tttactgcca
     1801 atgcacctat cattttataa tttgccaaag gaacatctcc cgctccggca ggttttgtta
     1861 cttcaggatt ctgcatttct acagaatatt tgtctacatc cgttattttc attcctgctt
     1921 tatctaatga agatactaag gatgttataa ctgcctgagg agatgagcct gttcctacag
     1981 tgtgtcttcc cacaaggtca agattaattt ccggattcac accgtcattt tcgcctatca
     2041 gcacagcaaa cccgccaatg gtatcctcta atatggggta tcctttttta atgtggtcct
     2101 taccgttcat tcctaatttt gcagtgctac cccctgcaga tacaataact tttttgaata
     2161 ctcctgcttt taccaatgct gcagcttcta ctatagcatg agtaggggct gcgcaaaaac
     2221 ctcttgtatc acaaccggta gcattaacga gtcctgctac ttcagcacat gactttgcaa
     2281 aatttcctcc gcctctttgg ttcatatcgc cgcatgcttc ttcgctgcat tcgatgatgt
     2341 attcaatttc ttctttgttt atgttgtttt tgcttataag atgtaataaa gcaagaacat
     2401 ttgaagcttt tgatacaaga ttttcaaaca ttacatgtcc gcttaagttg atatcaatat
     2461 catgcgccct tttaacgcat cctaccagcg ctccattatt atatataggc tcggcatgct
     2521 ctgcttccac atattttttt attgtatcaa actcttctcc ttctttgatt cttcctatta
     2581 aactatcgtc aataagagga tgattcataa gttttgcttt aacagcggaa acgaattgtt
     2641 tttcaagttt aactaaatca aatacatcac agatttgcat aagaactata aattcatcct
     2701 gaggcataat ttcaccgaat ttaccttctc ttgtcgctcc ttccaaaggc ttgtcaaacc
     2761 aaggcatttc atatttgctt aattcatcag gtgtaatatt tcctatatat gtttgattag
     2821 gcaaataact tacgacttgt tcatacgttc tcagatgatt tctaatttcc tttaaatact
     2881 cggaatttgg atttaccgtt ctttcggttg tctgtgtagt accgttatta ataaccatat
     2941 caggtgcaaa aaccattatg taactgcttc ctaaaattac actgttcata aaaacctcct
     3001 ttatataaat tttaatgatg atccacatca ttccaaataa ttgttcgtat tcaaaatttg
     3061 cttcgcaggt tctacgaagc aaattttttc atgttctatt ttgtgttaaa taacgaggtc
     3121 atacctcttc cctcaacctt ataagtactt agaaaattct tctcttattg cagtcatttc
     3181 actaattatg tcatcaacat ccaataccat ttccatcata cccacttgtt catcatatac
     3241 tgattcatca accagctctt tgatttccgg ttcgcaaata tgaaatacgc tgagtcccaa
     3301 ctgaactcct gtcaatggac ctgcaaaagt agggtctccg tttgttacgg tttctgcagc
     3361 aagacctgct gcttcgcctt ctgctgctcc aagtataacc actatatttt ccgggccata
     3421 cttttcagca aattctttaa cccttttttg attttccaaa tccattgcac ctgcgctagt
     3481 tcagacaaag cattctgtgg atgagaatac aatttcgccg ccggcagttt ttacacattc
     3541 ttcaattgcc tgaccgggta ttccatctct gtcgcctatt atgataattt tcttgttctc
     3601 taataagctc ataatttcct cctatattat tttgtatatt taaatgtatt tttgaatcat
     3661 tgcttctata ttttcaggag ttgcatcatc tttaaccaat tcttcaattt tttctccatc
     3721 cttatatatt gcaattacag gtaaacctaa taccttttgt ccaatagcta cccttcttgc
     3781 tttggtagta ttcaatttag taaattttaa tttatcacca tatttttccg caagtttttc
     3841 aacatgaggc attaaggctt cacagggagc acatccgtct ccgaaaaagt caacaaatat
     3901 gtatccttcg ccctgtaata cttctgtttc gaaatttgtt ttatccaaca ctaacatagc
     3961 atacctccta cagtgtttca atatattttt ctgcctgaac tgcagctatt gctccgtcag
     4021 ctgccgcagt aacaacctgc cgtaagcttt taactctgac atcgccggct gcaaaaacac
     4081 cttcgatatt tgttttcata ttatcgtcgg ttacaaggta tccattttcg atatctaata
     4141 ttccttcaaa tattttagtt tttggctcat aaccaatgaa tacaaaaata ccgaatgtca
     4201 tatcatcttc atcggcaact atttccgtca attccttagt tttaacattt tctaaaacta
     4261 ttgaattgac aataccatca ccttttattt cctttactac cgaatcccag ataaaatgta
     4321 ttttgggatt tgcttccgct ttttctcgaa tcgatttggc agctctaagc tcatttcttc
     4381 tgtgaataat tgatactttt cttgcaaact tagttaagta caaagcttct tcaacagcag
     4441 tatcgccgcc gcccactaca aatacttcta aatcttcaaa gaaagcagca tcgcaagttg
     4501 cacagtatga tacccctttg cctgtgtatg ttttttctcc gggacatccg ataggccttg
     4561 atgtagcacc gcttgctatt attactgctt tgcctttgta ttctcccttt ttgccgataa
     4621 gtattttttc tttgccttca agcttcactt ccacaatttc atcatatact ttttctacgc
     4681 cgaaatgctt agcctgttca gccatcctgt caactaatgt aggtcctgat tctccttcta
     4741 aacacccggg ataattttca acttctgatg tgagaacaat ttgccctcca tctttttctt
     4801 tttcaataat taatgtacta agccttgctc ttcccgcata aataccggct gataatcctg
     4861 ccggacctgc tccgataatc aatacatcat aaactttact catattccac ctcttttctg
     4921 tctaatatta aattggtaat tatatacata ctgcttttat tcgcaaattt atttctaatg
     4981 ccattgcaat gaataagcac tgtgattgtc atcctgagca taagcgaagg atatcagttt
     5041 ttataaagat gagattcttc gagctaaagc tctcagaatg acacgtagct tattaatatt
     5101 tttcttggtg tagtaaaaac gtgataatta tgagctaagt gaagaatctc acgcatattg
     5161 ctgagatcct tcactttaga tcaggataag cgactgacta caaatcaaag atttgggtca
     5221 tctgtttact tttcgaatac agtttgctct gtaacttcag tttctaaagc ttttaatgct
     5281 cttgtcagca attttcttct tatttctttt tcttcttcta tagacaatga aggattcccc
     5341 aaggggtgag gtattgctat tgtcggaaca attctgttag ctccaacagt taatgaaata
     5401 ggaactatag tacacatatg taccacagga attcccgctc tttcgatttc ttttaccatc
     5461 gttgcaccgc aacgtgtaca ggtacctcag gtagatgtga gtataactgc atcaactccg
     5521 tcagctataa gttcttttac atatttttcg gcaaaacctt tagcactctt tacggcagtt
     5581 ccgttaccaa ccgtggtata gaaatatctg tgaagacttc ctattttgcc ttccttttca
     5641 aattctctca taatatcaac aggcaatact ctatcagggt ctaaatttgc ataaacaggg
     5701 tcatatccgc cgtgagctgt ctgatatgtt tcttcggtta aatccataac tccttcgata
     5761 tcatatttac cgtatttaga agcacttgat gattcaatat ggtcaggatt tcctttcgga
     5821 acaattcctc cggaagtaac caatgcaatt tttgcttttg taatatcttt tatagccgga
     5881 tttggaggta ctctgtcaaa atcaggcatt ggaaattctg tttcaaattc tttgttgttt
     5941 aattttgcaa gaagcatttt aacagctctt gttgagcctc tttctttttc aaagaaatta
     6001 actcttttac cgcttggtat gtagccttct tctattgatg cgccgatttt ttctcccttt
     6061 gcaagtttaa gtcctaatgg agccattttc ttaacagcat ctctcattcc tgcagcagaa
     6121 ttctttgtgg atactatata gatgtcgttt ttaaacatat ctgctccggg attttccaca
     6181 tacattcccg ttataacagg gatattcaat tcatccttaa ctgtctttga aatagcgccg
     6241 caagctacac cgtatctgcc agcattaaat gccggtcctg caataaataa atccgggttg
     6301 tatttcttga ccatttcaat tatggtattt ttggcttcat ccatattctc gttgaaatat
     6361 gagtctccgc atataattgt tgctacaatt tctgcttcat ccttaaatgc ttgttgaaat
     6421 gcaagacccg gtcctactac accttctctt acttcagcag gaatgtgagc catttcttct
     6481 ccgcctacat tggcaaagaa ttgattaata taatgaacta cttttagcat tattttctcc
     6541 tccttcctat cttgcactaa gtttattgaa gcccatttca ttagtagcac ctgtaataac
     6601 ctgcaattcc gctgttatgc ttccatcttt atgcaagctt ccatcaaatc ctccggctat
     6661 tacatcagca acctcaacat gaccgattat tttttccata ggaggcagct caattacttc
     6721 atttgcattt cctgcggaaa ccactgcatt agctgataca tctgcatctg ccaatgactg
     6781 agaagtaccg tctctgcctg catattcatc tgtaacaaga actgttttaa tacctttagc
     6841 ttcaattttc ttacagttca ttatcaagtc cgtatcagga tttccaaatc cttcttctga
     6901 tacaataaca ccgtcaacac caagaaatcc cgcaagtttt gcactccagt tggatgatct
     6961 ttccttatcg gcaagataaa cattttcgtt agttatgatt actccgacaa aattcaaggt
     7021 cttgccatgt tctttaaaca aatcatatat gataggattg tttaaatgat gatatgtagt
     7081 attcttatcg catgcagaaa cacagttacc gcttactatg gcaccatcca taacttcggt
     7141 aggataaatc atggttgtta atgtttgttt tgcatcaact ccataaacat aagtatcatg
     7201 caaaagacct tgactttgca gcatgaatac ataagctact cttggtagtt cgggatacat
     7261 ttttatccct tcaaccaaat taggtgtttc aaatacttca acttcatcag gagttaaatc
     7321 ctttgcaaga gctccaagat ataatgctac cttgtatcct gcaaatctta gggctttttc
     7381 atgctcgtgc tgctttaatc cgtcaatagg ctcgcaaatt ataactaagt tgttaagctt
     7441 ggaaaaaggt gtatattcgg ctcccgttcc gctcatatca atgatacctt cttggaaacc
     7501 tactattttg cctgtagtta caacagctgc acccttcaaa acatttgttt tgcctgagcc
     7561 tacagtatct actttgctaa ccattcccgg gaatatgcct ccggggcctt caaccttaac
     7621 tcttggctca ataacatctt taacaggcat tatgcgaaca ctttctcccg gctttgccag
     7681 ttctacatct gctgttttga aattttgatc ttctagaatt aatgctttta cttcttcctt
     7741 gttaacgtac aacgcaccg
//
LOCUS       DAOSOW010000033         7749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_9406_length_7749_cov_3.909410, whole genome shotgun sequence.
ACCESSION   DAOSOW010000033 DAOSOW010000000
VERSION     DAOSOW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9406_length_7749_cov_3.909410"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2487)
                     /locus_tag="PLD67_01660"
     CDS             complement(<1..2487)
                     /locus_tag="PLD67_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21264.1"
                     /translation="MNKVKYCRKCGNTINNSVKFCRHCGALQNNQSKSPSVRDSQPSK
                     KNIKGPDVIDNSIKQKQKEKYFSDNETKTAKKKRGIFGKIAALLLIIFIAVNIINFLS
                     DKLNKPGSILTTGSKNENKSISEYVISDVKISDDDLNVPAQSGFVSPDEPEIRIENTY
                     INFTSYNIDVTREVKVKKLNQISDNEKGFTIQAYDFEIEGISEFNVPVYIEMPYYPAI
                     EDNLYEESTIFVQYYNENESKWEMIPSWINAEANTVSFLTNHFSPFGVFQDDNLEDAK
                     KVAHLFKYLGVYKGPTTPVYTTGRNIERYYQFVDEKTFQTYIDYKTVPVNDFATSFLN
                     VMNHGASAGEYSLYALSPEIYKDTINGFSKAGKLFVLSKIAYQWYNDVATQDIIMDNI
                     FDLTEIAVGTVGTLAAAPELTVIAGGVWLTGLTYDVGTTAYEYMSENEPRYLAYRIAS
                     ESGYIKYLPDEEICVYWREIEDPSYSEYDKELAKSRNRSIKLSGEYGWAQAFNAIQKK
                     YKENPIEMTKALERLLESYLDVFWYHFGYAELTDILKSVETGIIFKSTLYDEWEWPVE
                     SEIQEYKANYRQKMKEYLRPIMKAMAQKALLELNELTLKEVVKLWDVVNMEIDFQIVD
                     ENLKEGEFFPSSPLSDMTIKLSKLSDEAIADEWICRERRERSNSVFTCNIYAYIKAGC
                     PTNVEFYEKNTETGVQELVISKEFTLSNPTVISIDKRMEDINGIYTGKAIGGNYTTGE
                     EMDLYYKAIRIESNKEGILIGPCLENGDYDPDYMVQCVLNKETNQYEGKVKRESGNQW
                     MELSFIAEIYEVYKKPRARVGYSQTISTRIG"
     gene            complement(2523..3395)
                     /locus_tag="PLD67_01665"
     CDS             complement(2523..3395)
                     /locus_tag="PLD67_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT21265.1"
                     /translation="MFCEKCGYENKDDALFCENCGEQINNKETEMVLESDDAVAVETA
                     EIQETSVADKIEGIQETEEIQESEEIQEKAEFDETAIEQSTIISKNITKNSTGSYSWM
                     YEFSLWKNPAVLITAFKVSLIALMVPVLLMFFLTLGDGIGEAFKLLLSMLGIGAVLMT
                     VLMVAGYVMLSIAYGGKYYVLFKMDKKGVNHIQLDKQHKKAQALGFLTALIGLSSGNI
                     SAAGAGLLGASRKSLYTNFKKVSSVKFSPRMNTIYLNESLNKNQIYAEKEDYLFIKEF
                     ILENCPKNIKIIEK"
     gene            complement(3432..3782)
                     /locus_tag="PLD67_01670"
     CDS             complement(3432..3782)
                     /locus_tag="PLD67_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21266.1"
                     /translation="MAVNYLPDTEEIHYSSAVISEYSPDSAHMGANTSVLLTHQYTKY
                     DGEKYFYDEEDSTDMVLGSYGEGILQMALADDKVMDVIFWEKEGKQYGQSYLFRDDDN
                     DGFADLVFSMAFVR"
     gene            complement(3890..4345)
                     /locus_tag="PLD67_01675"
     CDS             complement(3890..4345)
                     /locus_tag="PLD67_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT21267.1"
                     /translation="MFCESCGKNIPDESRFCEYCGSEVIPEIFIQSDEDTDNSIHSEQ
                     GNASYNKQERGSRYEQEIEKEYEYPRTPKPKKSGLIKKAAVFLVLAVVLVGGYSLLRQ
                     FIGKNSQKTVENSTKPPMGRESIPQEEQPPPTVPKTMDLRTIYLQPMIL"
     gene            complement(4356..4538)
                     /locus_tag="PLD67_01680"
     CDS             complement(4356..4538)
                     /locus_tag="PLD67_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21268.1"
                     /translation="MIKKNVAGLWLLFMAFTAFGGFYLYYKLSGDAEGIEAGKIVGGM
                     LLALSVISFIKIRPWK"
     gene            complement(4561..5631)
                     /locus_tag="PLD67_01685"
     CDS             complement(4561..5631)
                     /locus_tag="PLD67_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT21269.1"
                     /translation="MNKITYSISKNTVLSAGKHFGIQANRFAEYFGFTESSKSTEIVE
                     GWEDLLPLILKPRNFTGMTALFEDDSLLFTNIVTGEEDAAFCGEEDDILKVMPMSQSE
                     IIELLASYLGEPEDRQKIKQELSGDALICLLSIGDSIKRAKLENILDPSKIEFEITDK
                     ILQNEFLYNSEGRNLRWASAFLADLRWKEKPINFEKSLKELSKAGIIIYRDSRVELSE
                     NGEIFFDELLKRKTILGLRSVFYHEGVINYLAMALMRTENYLWFLDVTDNACIMSLNY
                     EELKGMIGTMISPGEEPPEIEADEEVEQTISKAKMKAETKAETESINKDRENPTTPKF
                     CRNCGEKLSIGSKFCKKCGNKI"
     gene            complement(5628..>7749)
                     /locus_tag="PLD67_01690"
     CDS             complement(5628..>7749)
                     /locus_tag="PLD67_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21270.1"
                     /translation="GAIGDDYADELAKKLKILRSQKKIDGEFNKDDLKKFQKTFNRWT
                     RGEIAGSRGLPKAENDWDIYKQGLALSGEEIARGDSWKAMGLRVAVGLLTGSLAARGI
                     ISLAGAAVVEAGLELGQAGYTMKDYVDKGGDDWTEGAKLAITKTLVGEGIGRSIGLGL
                     RGLGLAVSKTGQALSKTKAGKYIVDGISDAVEKTSRVLNANVGDVPKVIKQISKEASE
                     SAAAAKKAASMAGKLKTSAANKAAQTADDAAKKAAQSTEDAVKKSAQSTEDAVKKAAP
                     PKNVKDTDAYKQASRQVDDSLNKSKAAAAEKIEKYKNSVNKNPKLAARKAAYEEGGKI
                     GLEKVEKLEQARKALAGNPDSPQLKKEFESAVKDVQINKHAQKIMNEKKLTGNDTRQG
                     FNDFKVKQDAIVSLNTSERLALELGLDPKNVSNVQATNSMDTSGVKTIKGVAVKSDFK
                     TPKTTAPRKFTEADIDLNSLEKKSGEKISIDLDQTYRMEIELKDGTVIAKDIPAYKVK
                     SIQNEEVYKVWNDGQLPLNPDGSVNHEAVEKFAEDMDYTVVDARSADAYGTLGDLEQA
                     LKNDGTIRKYNDPEVVSKTISYKSDEWIRRSKGKTKVGEIIDAEADVSEGIRQSTKQF
                     DSQYVYQVKAINADAGKTKIVINEKLAEGMEVLKQIGFGEGKLSPAEAEAILKEMGTN
                     SEEVMKLLADEVEIINRFLAGGKK"
BASE COUNT         1984 a         1695 c         1174 g         2896 t
ORIGIN      
        1 gccaattctt gtggatatgg tttggctata tccgaccctt gctcttggtt ttttatatac
       61 ttcatagatt tcggcgataa aactaagctc catccattga ttgccggatt ctcttttgac
      121 ttttccttca tattgatttg tttctttatt taaaacacat tgaaccatgt aatcagggtc
      181 atagtctccg ttttccagac aggggcctat gagaattcct tctttgtttg attcaattct
      241 gattgctttg tagtagaggt ccatttcttc tccggtagta tagttgcccc ctatggcttt
      301 accggtataa atgccattta tatcttccat tcttttatca atagagataa cagtcggatt
      361 tgatagagtg aattccttgg aaattactag ctcctggacg cctgtttctg tatttttctc
      421 ataaaattct acatttgtcg gacatcccgc tttaatataa gcatatatat tacaggtgaa
      481 aactgaattg cttctttctc ttctttccct gcatatccat tcgtcagcta ttgcttcatc
      541 acttagtttt gataatttaa ttgtcatgtc agacaatgga gaagatggaa aaaattctcc
      601 ttcttttaaa ttttcatcca caatttgaaa atcaatttcc atatttacaa catcccaaag
      661 ttttactacc tcttttaagg taagctcatt taattccaaa agagcctttt gagccatggc
      721 tttcatgatc ggtcttaaat actctttcat tttttgcctg taatttgctt tatactcttg
      781 gatttcagat tctacaggcc attcccattc atcatataaa gtcgacttga aaataatgcc
      841 tgtttcaaca gacttaagaa tgtcagtaag ctcagcgtag ccaaagtggt accagaagac
      901 atctaaatag gactccaaga gtctttctaa tgcttttgtc atttctatgg gattttcttt
      961 atattttttt tgtatggcat taaatgcttg tgcccacccg tattctccac tcaacttaat
     1021 agacctgttt ctgctttttg ccagttcttt atcatattcc gaataagatg gatcttctat
     1081 ttctctccaa tagacgcata tttcttcatc cggcaaatat ttaatgtaac cggattcact
     1141 tgctatgcga taggctaaat atcttggttc attctcagac atatattcat aagctgtcgt
     1201 tccaacatca taggtcaaac ctgtcagcca tactcctcct gcaattactg tgagttcagg
     1261 tgctgctgca agggttccaa ctgtgcctac agctatttct gtaaggtcaa agatattatc
     1321 cattataata tcctgagttg ctacatcgtt gtaccattga tatgcaattt ttgaaagtac
     1381 aaaaagcttc cctgctttgc tgaaaccatt aatagtatct ttgtaaattt caggagataa
     1441 agcatataaa gaatactctc ccgctgatgc tccatgattc ataacattta aaaaacttgt
     1501 tgcaaagtca tttaccggta ctgttttata atcaatatag gtttgaaatg ttttttcatc
     1561 aacaaattga tagtatcttt cgatatttct tcccgtagta tatacaggtg ttgtagggcc
     1621 cttatatact cccaaatact taaataaatg cgctactttt ttagcatctt ccaaattatc
     1681 atcctgaaat acgccgaagg gtgaaaaatg atttgttaga aatgatacag tgttggcttc
     1741 ggcatttatc caggaaggta tcatctccca tttgctttcg ttttcattgt aatactggac
     1801 aaatatggta ctttcttcat aaagattgtc ttcaattgcc ggatagtatg gcatttcaat
     1861 atagacaggc acgttaaatt cagaaatacc ttctatttca aaatcatagg cctggattgt
     1921 aaagcctttt tcattgtcac tgatttgatt taattttttt accttgactt cccttgttac
     1981 atctatatta taagaagtaa aattgatata ggtgttttcg attcttattt ccggctcatc
     2041 agggctcacg aatcctgact gggcagggac atttaaatca tcatcactta tttttacatc
     2101 acttataaca tattcactta tgcttttgtt ttcattcttg cttccggtgg ttagaatact
     2161 gcccggttta ttgagcttat cagataagaa gtttattata ttaactgcaa taaaaattat
     2221 cagcaaaagt gcggctattt tcccaaatat tcctcttttc tttttggctg tttttgtttc
     2281 attatcactg aagtattttt ctttttgctt ttgcttaatg ctattatcaa taacatccgg
     2341 tccttttata tttttcttcg aaggttgtga atctctgaca gatggtgatt ttgactgatt
     2401 gttctgcaag gcaccgcaat gtctgcagaa tttaacgcta ttattgattg tattgccaca
     2461 tttcctgcaa tattttactt tattcaatgc aactccccct tatatttata ttttgagact
     2521 tgctattttt caataatttt aatatttttc ggacaattct ctaaaataaa ttcctttata
     2581 aagagataat cttctttttc cgcataaatc tggtttttat ttaaggattc atttaaataa
     2641 attgtattca tcctcggact gaatttaacg gagctaacct ttttaaaatt tgtgtataag
     2701 ctttttctgg atgctcccaa aaggcctgct cctgctgcag aaatattccc tgaagaaagg
     2761 cctattaaag ccgtaagaaa tcccaaggcc tgtgcttttt tatgttgttt gtccaattgt
     2821 atatgattaa cacccttctt atccatttta aacaatacat agtatttacc gccatatgct
     2881 atgctgagca ttacatatcc cgcaaccata agaacagtca tcaatacggc acctattcca
     2941 agcatggaaa gtaaaagctt aaaggcttcc cctataccat ctcctaatgt caaaaagaac
     3001 attaataaga ccggaaccat caaggctata agcgaaactt taaaagctgt tatcaggact
     3061 gcaggatttt tccacaatga aaattcatac atccagctgt agcttcctgt actgttctta
     3121 gtaatatttt ttgaaataat agttgattgt tctatcgctg tttcatcaaa ctccgctttt
     3181 tcttgaatct cttctgattc ttggatttct tctgtttctt gaatcccttc tattttatct
     3241 gccacgcttg tttcctgaat ttcggctgtt tctacagcca ctgcatcgtc tgattctaat
     3301 accatttctg tttccttatt gtttatctgt tcgccgcagt tctcacaaaa tagagcatca
     3361 tccttatttt cataaccgca tttttcacaa aacatatcta cctcctgctc tcttatataa
     3421 taagcaaatt ttcatcgcac aaatgccatt gaaaaaacca aatctgcaaa tccgtcattg
     3481 tcatcatccc tgaataaata actctgcccg tactgttttc cttccttctc ccagaaaata
     3541 acatccatta ctttgtcatc tgccaatgcc atttgtaaaa ttccttctcc atagctgccc
     3601 aataccatat cggttgaatc ttcttcatca taaaaatact tctctccatc atattttgta
     3661 tattgatgag ttaataaaac cgatgtattt gctcccatat gggccgaatc aggactatat
     3721 tcactaataa cagctgagga ataatgaatt tcttcagtat ccggcaaata gtttacagcc
     3781 ataagtttcc actcccccaa tatgtccatg taagaaagtt caacagctcc ttcaggtatc
     3841 tcatagtaat attcctcaaa atgccaattg aaatcatcct catttattat tacagaatca
     3901 ttggctgcag gtaaattgtt ctcaggtcca ttgttttcgg gaccgttggc ggaggctgtt
     3961 cctcctgagg tatactttcc ctgcccattg gtggtttagt tgaattttca acagtttttt
     4021 gggaattctt gcctatgaat tgtcttaaaa gagaatatcc tccgactaat acaacagcta
     4081 aaacaagaaa tactgctgct ttttttatca agccggattt tttaggcttt ggagttcttg
     4141 gatattcata ttccttttct atctcttgtt cgtagcgcga ccccctttcc tgtttattat
     4201 atgacgcgtt tccttgttcg gaatgtatac tattgtctgt gtcttcatca gattgaataa
     4261 aaatctcagg aatgacttca cttccacaat attcacaaaa cctgctttca tcaggtatat
     4321 ttttaccgca tgattcacaa aacatatttt cacctctact tccacggtct aatttttata
     4381 aagctaataa cagacaatgc taaaagcata ccgcctacaa ttttgccggc ttcaattcct
     4441 tcagcatcac cacttaattt ataatagaga taaaatccgc cgaatgccgt aaaagccata
     4501 aatagcagcc atagccctgc aacatttttt ttaatcataa taattcactc tgaacgatga
     4561 tcaaattttg tttccgcatt ttttacagaa ctttgatcct atgctcaatt tttccccgca
     4621 gtttctacag aatttcggtg ttgtcgggtt ttcccggtct ttattgatgc tctcagtctc
     4681 tgctttagtt tcagctttca ttttggcttt acttattgtc tgttcgactt cttcatctgc
     4741 ctctatctca ggtggttctt ctcccggtga gatcatagtg ccaatcattc ccttaagttc
     4801 ctcatagttt aaagacatga tgcacgcatt gtcagtaaca tccaaaaacc acagatagtt
     4861 ttctgttctc atcaatgcca ttgccaaata atttataaca ccttcgtgat aaaaaacact
     4921 tcttaagcct aatatggttt ttctttttaa aagctcatca aagaaaattt caccgttttc
     4981 tgatagttca accctgctgt ctctataaat tattatacct gctttagaaa gctccttaag
     5041 cgatttttcg aaattaattg gtttttcttt ccagcgaagg tctgccaaaa atgcagaggc
     5101 ccatcttaag tttctacctt ctgaattgta gagaaattcg ttttgcaaaa ttttatctgt
     5161 tatttcaaat tctatttttg aagggtccag gatattttca agctttgccc tcttgatgct
     5221 gtctcctatg gatagcaagc atattaaggc atcacctgat aattcctgct ttattttctg
     5281 cctgtcttct ggttctccaa gatatgatgc taaaagctct ataatctctg actgggacat
     5341 gggcattact tttaaaatgt catcttcctc accgcagaat gcagcatctt cttcaccggt
     5401 aactatattg gtaaaaagaa ggctgtcatc ctcaaatagg gctgtcattc ctgtaaaatt
     5461 acggggttta aggataagag gcagaagatc ctcccatcct tctactatct ccgtgctctt
     5521 ggagctttct gtgaatccaa aatattctgc aaaccggttt gcctgaattc caaaatgttt
     5581 tcctgcagat agtacagtgt tttttgatat agagtatgtt atcttgttca ttttttgcct
     5641 cctgctaaaa atctgttgat aatttcaact tcgtccgcta aaagcttcat gacttcttct
     5701 gagtttgttc ccatttcttt gagtatggct tctgcttcag cagggcttag ttttccttcg
     5761 ccaaatccta tttgctttaa aacttccata ccttcagcca atttttcgtt tataactatc
     5821 tttgttttgc ctgcatcagc atttattgcc tttacttgat acacatactg gctgtcgaac
     5881 tgttttgttg attgtcttat gccttctgaa acgtctgctt cagcatctat tatttctcca
     5941 accttcgttt ttcccttgct tctacgaatc cattcatcag atttatatga aatagtttta
     6001 cttacaacct ctgggtcatt gtattttctt attgtgccgt catttttcaa tgcctgttcc
     6061 agatctccca gggttccgta agcatcagcg cttcttgcat ctaccaccgt ataatccata
     6121 tcttcggcaa acttttcgac agcctcatga tttaccgaac cgtcagggtt caaaggaagc
     6181 tgtccgtcat tccatacctt gtatacttct tcgttttgaa tgcttttaac cttataagca
     6241 ggaatatctt tggcaattac agtgccatct ttaagctcaa tttccattct ataggtctga
     6301 tccaaatcta tgcttatttt ttctccactt ttcttttcaa gagagtttaa atcgatatcg
     6361 gcttccgtaa atttccttgg tgctgtggtt tttggagttt taaaatcact tttaactgca
     6421 acacccttta tagttttaac tcccgatgta tccatggaat ttgtagcctg gacattgctt
     6481 acatttttgg ggtcaagacc taattccaat gctaaacgtt cacttgtatt aagtgatact
     6541 attgcatcct gttttacctt aaagtcattg aatccctggc gtgtatcatt gcctgtcaat
     6601 tttttttcgt tcattatttt ttgtgcatgt ttgtttattt gaacatcttt aactgcactt
     6661 tcaaattctt ttttaagctg tggagaatca gggttgccag ctaatgcctt ccttgcctgc
     6721 tcaagctttt caaccttttc caatccaatt ttaccgcctt cttcataagc tgctttcctg
     6781 gctgctaatt taggattttt atttacacta ttcttgtatt tctctatttt ctcagctgca
     6841 gcagctttgg atttatttaa actatcgtca acctgcctgg atgcctgctt gtatgcatct
     6901 gtatccttca catttttcgg tggtgcagct tttttgactg cgtcctcggt gctttgagca
     6961 gattttttga cagcatcctc ggtgctttgg gctgcttttt tggctgcgtc atcagctgtt
     7021 tgagctgctt tgttagcagc tgatgttttt aattttcctg ccattgaggc tgctttttta
     7081 gctgcagcag ctgattcact tgcttccttg ctgatttgct ttattacctt tggcacatct
     7141 cctacattgg catttagtac tcttgaagtt ttttctactg catctgaaat tccgtcaaca
     7201 atatattttc ctgctttggt ttttgacaaa gcctgccctg ttttagaaac agctagacca
     7261 agccctctta atcccaaacc tatagacctt cctatacctt cgcccaccaa agtctttgtg
     7321 attgcaagct tcgctccttc tgtccaatca tctccaccct tgtcaacata atccttcatt
     7381 gtataacctg cctgaccaag ctccaaaccg gcttccacaa cagcagcacc ggcaagggaa
     7441 attatacccc gggctgcaag actgcctgtt aaaagcccca cagccacgcg aagccccatg
     7501 gccttccagc tgtctcccct tgctatctct tctcctgata atgccaatcc ttgtttgtat
     7561 atatcccaat cattttctgc ttttggcagt cctctgcttc ctgctatttc tcctcttgtc
     7621 catctgttaa atgttttctg gaattttttt aaatcatctt tattaaactc accgtcaatt
     7681 tttttctgac ttcgaagtat tttaagtttc ttcgctaact catctgcata atcgtctccg
     7741 atggctcct
//
LOCUS       DAOSOW010000034         7686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_9512_length_7686_cov_4.424584, whole genome shotgun sequence.
ACCESSION   DAOSOW010000034 DAOSOW010000000
VERSION     DAOSOW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9512_length_7686_cov_4.424584"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="PLD67_01695"
     CDS             <1..239
                     /locus_tag="PLD67_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HOT21271.1"
                     /translation="KIITDFKSYLQEYYQSKSQSCKIRYVVTKEEGPDHDKVFHVNVM
                     VNKNVAGKGVGKNKKIAEQNAAKDALIQEGCLNE"
     gene            232..1329
                     /locus_tag="PLD67_01700"
     CDS             232..1329
                     /locus_tag="PLD67_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT21272.1"
                     /translation="MNKKIKIIPIFVPHVGCPNNCVFCNQRRITGQKNFTVDGEYVHK
                     IVKRYIETIDGNTNTEIAFFGGSFTAIDTKIQEELLKAAKHYKDLKIISSIRCSTRPD
                     AINDEILMLQKKYGMDIIELGIQSLDDEVLKISNRGHNKKQSIDASRLIKEYGFILGH
                     QIMPGLPGSSRIKDIKTCIESIEMKPDIVRIYPTLVIKDTELFDMYEKGLYKPLSLDE
                     AVEISAYIYSLYSINDISVIRIGLQNTESINNDKDVMAGPFHPAFRQLVEERIYYYAL
                     GSKLCKVNTKELGESITIHAPDNLISSIAGQKRSNINKLKERFSIKQISFKKKKDDII
                     EIYNKNKRLFSFKKSEVFQNYLNMQQGDLCI"
     gene            1320..4901
                     /gene="smc"
                     /locus_tag="PLD67_01705"
     CDS             1320..4901
                     /gene="smc"
                     /locus_tag="PLD67_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HOT21273.1"
                     /translation="MYLKKIYVNGFKSFAEKTEIEVEKGITAIVGPNGSGKSNISDAI
                     KWVLGEQSAKSLRGGKMDDVIFAGTAKRLPLGYAEVRLTFNNESGIIPLEYKEVSISR
                     KLYKTGESEYYINKQQCRLKDIKELFMDTGIGREGYSFIGQGRVEDILSPNSDVRRQV
                     FEEASGIVKYKTRKEESERKLEKTKNNLDRVEDIIYELKERIEPLYEQSIRAQKFIEI
                     RDNLKKYELNYLAHEYEKHCEQLKALKNQRDMSLEEKLRLQKRRDYYAEIIESQKEKI
                     NDLQNKINETEALRDSKNKALEKYKSLRQVHKEKKLLYSSNIDNIKNEIGELEKRNLE
                     LEEKINVLIQEKVSCEEIYKRDIEKFESLNSDIKEYKEEIDEIVQSIEQQKNELFELH
                     KDINKNNSNKNTIESLIINNGDRIKHLDQEVNSEEEERQKRIETVNNLQEEQRITREL
                     LIKKKAVLKSILEELNNAEKNKERLNLHISKLNEEYNGTKSKINILTNMEAYYEGYYK
                     SIKTVMNNKDKEPVLNSILGTVADVIKTEKKYEKAVEIALGSAIQNIIVNTDKEAEEI
                     IEYLKKNKIGRVTFLPINMLTERSLNDFEREVLKEECVIDTADNLIQTNSKYEKVIKN
                     LLGRIIIADNLNNGLKISKKLGNTVKIVTLQGDVINAGGSVTGGHISSNQALLGRKRE
                     IDELKAHLDKLSEELKEKNDSLKLLNFKIKELNNSVEEIKSFINEKNNYYEMNNSKIK
                     MLSEQNEKTTAAINKYKEEIHFIQSEKQKYENDLRYLNAETEKLKLLVEQKEKSIEET
                     ILMNNSNKLKYDEYNNTISIQRDKVSNITQNIKLIEEKLNNIHMEIERNKNSIKYKEE
                     SLSSIEQEIEAAREKIIEFTECSDALEEEIKSLNESYIKEKETHKNYQNEIYDYQNKI
                     NESNKLITDILDEEMKLNVKIERESSKIEDISAKLWEDYEINYAMALKYKDDSISQTK
                     LYSEVNSCKKAIKELGNINLDSIEEYKEVRERYDFLTKQEKDLKDAIEQLNEVIKELE
                     IQMKEKFVEEFYNIRAKFSEVFKKLFNGGNGDIYLENEEDALNSNIEITVQPPGKKLS
                     KISLLSGGEKALTAIALLFSILKTKPTPFCILDEIESSLDDLNIFRFSQYLKEFSRET
                     QFIVITHRKGTMEYADTLYGATMEEKGVTKLVSMKLSDEQSIIGTHS"
     gene            4994..5911
                     /gene="ftsY"
                     /locus_tag="PLD67_01710"
     CDS             4994..5911
                     /gene="ftsY"
                     /locus_tag="PLD67_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOT21274.1"
                     /translation="MNNEKGFFAKLKEGLSKTKKNLTEQIEGVIFQYKKIDDNFLDEL
                     EEILITSDMGVGTTMDIINYIKEEAKKRKLEDTLELRDVIKEYLINVLKENERPDVTD
                     KQRVILIVGVNGVGKTTSIGKIAYRLKQSGNKVIVAAADTFRAAAVEQLKEWCQRAGV
                     DIIASEQGSDPGAVVFDAIQAAKARKADILICDTAGRLHNKKNLMNELSKIFKILDKE
                     FSEAHIDVYLVIDATTGQNGIVQAKLFKEACNINGLILTKLDGTAKGGIAFPIVNELK
                     IPIKYIGVGEQIDDLQDFNAQDFVNAIFE"
     gene            6215..6562
                     /locus_tag="PLD67_01715"
     CDS             6215..6562
                     /locus_tag="PLD67_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOT21275.1"
                     /translation="MSEKDFIYSILYDYYGDLLKKNQATIIEMYYNLDYSLSEIAEEM
                     NISRQGVHDALKRAEKNLAEYERKIKLYDKYVKFSEAAENIIQLSSKIDDKKYKDIIE
                     KIKLEALRIINEG"
     gene            6565..>7686
                     /gene="ffh"
                     /locus_tag="PLD67_01720"
     CDS             6565..>7686
                     /gene="ffh"
                     /locus_tag="PLD67_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOT21276.1"
                     /translation="MFENLSDKLQNALQKLRGKGKLTEKDIDLAMKEIKMSLLEADVN
                     FKVVKAFINNVKERSLGVEVMESLTPAQQVVKIVHEELAKIMGQGESKLKFNSNDITY
                     FMMCGLQGAGKTTATGKLARRLKKDGRRPLLVACDIYRPAAIKQLMVVGESVGVPVFE
                     MGDKKSPVTIAQEAINHARKHGNDVVIIDTAGRLHIDDELMEELVEIKKTVKPDEILL
                     LLDAMTGQDAVKVAETFNSYLDITGVILTKVDGDARGGAALSIRTVVDKPIKFLSVGE
                     KMDQLETFHPDRMASRILGMGDVLSIIEKAQGAFDEKQAIELQKKLKTQDFNLEDFLT
                     QLQQMKNMGPLDEILEMIPGLGGKKKLKGLKVDEKELVYT"
BASE COUNT         3215 a         1001 c         1522 g         1948 t
ORIGIN      
        1 gaaaaattat aacggatttt aaatcgtatc ttcaggaata ctatcaaagt aaaagccagt
       61 catgcaaaat cagatatgta gttacaaagg aagaagggcc tgaccacgat aaagtttttc
      121 atgtgaatgt gatggttaac aaaaatgtag cgggaaaagg cgtcggtaaa aacaagaaaa
      181 tagctgagca aaatgccgcc aaggatgctc ttatacaaga aggctgtttg aatgaataag
      241 aaaataaaaa ttattcctat ttttgtacct catgtaggtt gtcccaataa ctgtgtattt
      301 tgcaaccaaa gaagaattac aggacaaaaa aattttactg ttgatggaga atatgttcat
      361 aaaattgtta aaaggtatat agaaacaatt gatggtaata ccaatacgga aattgcattt
      421 ttcggcggct catttacggc aatcgatacg aaaattcagg aagaattatt aaaggctgca
      481 aaacattata aggacttaaa aatcataagc agtataagat gctccacgag acctgatgcc
      541 ataaacgatg aaatactgat gcttcaaaaa aaatacggaa tggatataat tgagttagga
      601 atacaaagtc tcgatgatga ggttttgaag atttcaaacc gagggcataa taaaaaacaa
      661 tcaatagatg caagccgcct cataaaggag tatggattta tattaggaca ccagataatg
      721 cccggacttc ccggaagcag caggataaag gacataaaaa catgtattga gtcaatcgaa
      781 atgaaaccgg atattgtgag aatttatcct acattggtaa taaaggatac tgagcttttt
      841 gacatgtatg aaaaaggctt atataaacct ctatctctgg atgaagcagt agaaatatca
      901 gcatatatat acagcttata cagtatcaac gatataagtg ttataaggat aggacttcaa
      961 aatactgaat ccatcaataa tgataaagat gttatggcag ggccctttca tcccgctttc
     1021 aggcaattgg tagaagaaag aatttactat tatgcattgg ggtcaaaact gtgcaaagtg
     1081 aatactaagg agcttgggga aagtatcaca attcatgcac ctgataatct gataagcagt
     1141 atcgctggcc aaaaaaggtc caatatcaat aaattgaaag aaagattttc aataaaacaa
     1201 atatccttta aaaagaaaaa agatgatata attgaaatat ataataaaaa taaaagatta
     1261 ttcagtttta agaaatcaga agtatttcaa aattatttga atatgcaaca gggggattta
     1321 tgtatttaaa gaagatatat gtaaacgggt ttaaatcctt tgcggaaaaa actgaaatag
     1381 aagtagaaaa aggtattact gccattgtag gaccaaacgg gagcggtaaa agcaacatat
     1441 ccgatgccat taagtgggtt ttgggggagc aaagtgcaaa atccctcaga ggaggcaaaa
     1501 tggatgatgt tatatttgcc ggaactgcaa aaagactgcc cctgggttat gctgaggtaa
     1561 ggctgacttt caacaatgaa tcgggtataa taccacttga atacaaagaa gtaagcatca
     1621 gcaggaagct gtacaagaca ggtgaaagcg aatattacat aaacaaacag caatgcaggc
     1681 ttaaagatat aaaagagctt tttatggata ccggaatagg aagagaaggc tattctttta
     1741 ttggacaagg aagggtggaa gacattttaa gccctaattc tgatgtgaga agacaagttt
     1801 ttgaggaagc ttccggaata gtaaaataca agacaagaaa agaagaatct gaaaggaaac
     1861 ttgaaaaaac aaagaataat ctggatagag tagaagatat aatatatgag cttaaggagc
     1921 gtatcgagcc tctttatgag caaagtatcc gggcgcaaaa atttattgaa ataagggaca
     1981 atctaaaaaa atacgagctt aattatttag ctcatgaata cgaaaaacat tgtgagcagc
     2041 ttaaagccct gaaaaaccaa agagatatgt cattggaaga aaagcttagg ctgcaaaaaa
     2101 gaagagatta ttatgctgaa attattgaat ctcaaaaaga gaaaattaat gacttacaga
     2161 ataagatcaa tgaaactgaa gcgctgagag attcaaaaaa taaagcttta gaaaaatata
     2221 agagcttaag acaagtacac aaagaaaaaa aattgttgta cagctcaaac atagacaata
     2281 ttaaaaatga aataggagaa cttgaaaaaa ggaacttgga attagaagaa aagattaatg
     2341 ttttaattca agaaaaagtt tcttgcgaag agatatacaa gagagatata gaaaaatttg
     2401 aatcattaaa ctcagatatt aaagaatata aagaagaaat tgatgaaatc gtgcaatcga
     2461 ttgagcagca aaaaaatgaa ttgtttgaac tgcacaagga tatcaataaa aacaattcaa
     2521 ataaaaacac aattgaatca ttaataataa acaacggaga cagaattaag catttagacc
     2581 aagaagtgaa ttcagaagaa gaagaaagac aaaagagaat agaaacagta aacaatctgc
     2641 aggaagaaca aaggattaca agagaactgc tgataaaaaa gaaagcagta ctgaaaagca
     2701 tattggaaga attaaataat gcagaaaaaa ataaagaaag gttaaattta catatttcca
     2761 agttaaatga agagtataac ggcacaaaat caaaaataaa tatcctgaca aatatggaag
     2821 catattatga gggatattat aaaagcataa aaaccgtaat gaacaacaag gataaggagc
     2881 ctgtattaaa cagtatattg ggaacggtgg ccgatgtaat aaaaacagaa aaaaaatatg
     2941 aaaaagctgt tgaaatagca ttgggctccg caatacaaaa cataatcgta aacactgata
     3001 aagaagccga agaaataata gagtatttaa agaagaataa aatcggaaga gttacttttc
     3061 ttcctattaa tatgcttaca gaaagaagtt taaatgactt cgaaagagaa gtactaaagg
     3121 aagaatgcgt aattgacacg gcagacaatt taatccaaac aaattcaaaa tatgaaaaag
     3181 tcattaaaaa tttgcttgga agaataatta ttgcagacaa cctgaacaac ggtctaaaaa
     3241 tatccaagaa gcttggaaat acagttaaaa ttgtaacact tcaaggagat gtaataaatg
     3301 cgggcggttc cgttacagga gggcatataa gctcaaacca agctctcttg ggaaggaaaa
     3361 gagaaattga tgaattaaaa gctcatttag acaaactatc ggaagaatta aaagaaaaaa
     3421 atgattcatt gaagcttttg aattttaaaa taaaagaatt aaacaattcc gtagaagaaa
     3481 ttaagagttt tattaatgaa aaaaataatt attatgagat gaataattca aaaattaaaa
     3541 tgctgtcaga gcaaaatgaa aaaactactg ctgctatcaa caaatataaa gaagaaattc
     3601 atttcataca gagtgaaaaa caaaaatatg aaaatgattt aagatatttg aatgctgaaa
     3661 cagaaaaact gaaattattg gtcgagcaaa aagaaaagag tattgaagaa accatattaa
     3721 tgaataattc caataaattg aaatatgatg aatacaataa tacaatttca atacaaaggg
     3781 acaaggtatc taatattact cagaatatta aacttataga agaaaaactg aataatattc
     3841 atatggaaat agaaagaaac aaaaattcca taaaatataa agaagaaagc ttatcctcta
     3901 ttgagcagga aattgaagca gcaagagaaa aaattattga atttacggaa tgctctgatg
     3961 ctttggaaga agaaataaaa tcattaaacg aaagctatat aaaagaaaaa gaaactcata
     4021 aaaattatca aaatgaaata tatgactacc aaaataaaat taatgaatca aataaactga
     4081 taaccgatat acttgatgaa gaaatgaaat tgaatgttaa aattgaaaga gaaagttcaa
     4141 aaattgaaga tatttctgca aaactatggg aagactatga aataaactat gcaatggcac
     4201 taaaatataa ggatgacagc attagtcaaa cgaaacttta cagcgaagtg aactcatgca
     4261 aaaaggcaat taaagaattg gggaatatta atttggactc catagaagag tacaaagaag
     4321 ttagagaaag atatgatttt ttaacaaaac aagagaagga tttaaaagat gccatcgagc
     4381 agttgaatga ggtaataaaa gagcttgaaa ttcaaatgaa agaaaagttt gttgaagaat
     4441 tttacaatat aagagcaaaa ttctctgaag tattcaagaa gcttttcaac ggaggaaatg
     4501 gagatattta tttggaaaat gaggaagatg cgttaaacag caacatagaa attacagtac
     4561 agccgccggg taaaaaattg agcaaaatat cattgctgtc aggcggagaa aaggcgctta
     4621 ctgcaattgc tctcttgttt tcaattttaa aaaccaagcc gacgccattt tgcatattag
     4681 atgaaattga atcatcgctt gacgatttaa atattttcag attttcgcaa tatctaaaag
     4741 aattttcaag ggaaacacaa tttattgtaa ttactcacag gaaaggaact atggagtatg
     4801 ctgacacttt atacggagca acaatggaag aaaagggagt tacaaaatta gtatcgatga
     4861 aactgtcaga tgaacaaagc attataggga cacacagtta agctatgggg ataaaccact
     4921 cggataggga tagtcgctaa agagatagcg gaagagggac agaagaatgt ccccaaataa
     4981 gatggaggca aatatgaaca atgaaaaagg cttttttgcc aaattaaaag aaggactttc
     5041 caaaacaaag aaaaacctta cagagcaaat tgaaggcgtg atttttcaat acaaaaaaat
     5101 agatgataat tttcttgacg aattggagga aatacttatt acttccgaca tgggtgttgg
     5161 aacaacgatg gacattatta attatatcaa agaagaagca aaaaagagaa aattagaaga
     5221 tacgctggag cttagagatg taattaaaga gtatctgata aatgttctta aggaaaatga
     5281 aaggcctgat gtaacagata aacagagggt aatattaata gttggggtaa atggtgtcgg
     5341 taaaacaact tctatcggca aaattgccta tagattaaag cagtccggca acaaggttat
     5401 cgttgctgct gcagatacat ttagagctgc agcggttgag cagcttaaag agtggtgtca
     5461 aagagcagga gttgatataa ttgcttcaga gcaaggctca gaccccggag cagttgtttt
     5521 tgatgcaata caggcagcaa aagcaagaaa agcagatata ttgatatgtg atactgcagg
     5581 aagacttcat aacaagaaga accttatgaa tgagctcagc aagattttta aaattcttga
     5641 taaagaattc agcgaagccc atattgatgt gtatcttgta atagatgcaa caactggtca
     5701 aaatggtatt gttcaagcaa agctgtttaa agaagcatgc aatattaacg gtttgatttt
     5761 aacgaaattg gatggaacag caaagggagg catagcattt cctattgtaa acgaactgaa
     5821 aataccgata aaatatatcg gtgtcggaga gcaaatagat gacctgcagg attttaacgc
     5881 tcaagatttc gttaatgcaa tttttgagta aacacgaaaa ataataaaaa tatccgctca
     5941 cggtttcgga cacgaataat tctatgcgct cactacggct aaaaacccta cagcttgcaa
     6001 actcgctctg ctcaaacagt gcaagctgct taacgggttt taagccttca ttcgctaaga
     6061 attataattg tgtcctgcaa atgttcgctt gatattttta ttattttttt cattataatg
     6121 aataatatgt ctcaatatat caaaaaaact attgacaaaa taatcaggat gcggtaaaat
     6181 tcactgtcaa gtaaaatact ttacaggggt tattatgtca gagaaggatt ttatatatag
     6241 catcttgtat gattattatg gagatctgct taaaaaaaat caagcaacca taattgaaat
     6301 gtattacaac ttggattaca gcttatcaga aattgctgaa gaaatgaaca tcagcaggca
     6361 gggagtacat gatgctttaa aaagggctga aaaaaacctt gcagagtatg agcggaaaat
     6421 aaaactatat gataaatacg ttaaattcag cgaagcagca gaaaacatca tacaattgtc
     6481 atcaaagatt gatgataaaa aatataaaga tattattgaa aaaataaaac ttgaagcatt
     6541 aagaataatt aatgagggtt aaaaatgttt gaaaacttat ccgataaact tcaaaatgca
     6601 ttacaaaagc ttagaggcaa gggaaaactg actgaaaaag atatagactt ggcaatgaaa
     6661 gaaatcaaaa tgtcacttct tgaagcagat gttaatttta aggttgttaa agcctttatt
     6721 aacaatgtga aagaaaggtc attaggcgtt gaagtaatgg aaagtttgac tcccgcacaa
     6781 caggttgtaa aaatagtcca tgaagaatta gccaaaataa tgggtcaggg tgaaagcaag
     6841 cttaaattca attcgaatga tataacatat tttatgatgt gcgggcttca aggggcagga
     6901 aaaacaacag ctaccggaaa gcttgcaaga agactgaaaa aagacggcag gagaccttta
     6961 cttgtagctt gcgacatata cagaccggct gcaataaagc agcttatggt agtgggagaa
     7021 agtgtaggag ttcctgtttt tgaaatggga gataaaaaat ctcctgttac aattgcacag
     7081 gaagcaataa atcatgctag aaaacatgga aatgacgttg ttattataga tactgccgga
     7141 cggcttcata tagatgatga gttgatggaa gaattagttg aaattaaaaa aactgtaaag
     7201 cctgatgaaa tacttttgtt attggatgca atgacaggac aggatgcagt taaagttgca
     7261 gaaacattca attcttattt ggatattaca ggagtaatac ttacaaaggt tgatggtgat
     7321 gcaagaggcg gagcagcgct ttccataaga accgtagtgg ataagcctat aaaatttttg
     7381 tctgtgggag agaaaatgga ccagcttgaa acatttcatc ctgacagaat ggcatcaaga
     7441 atcctcggga tgggagatgt tctatcaatt attgaaaaag cccagggtgc ttttgatgag
     7501 aaacaggcaa ttgaacttca aaagaaatta aaaactcaag atttcaattt agaagatttc
     7561 ttaacacagc ttcagcagat gaaaaatatg ggacctttgg atgagatatt ggaaatgatt
     7621 cccggacttg gaggcaagaa aaaattaaaa ggtcttaaag ttgatgaaaa agaattagtg
     7681 tatacg
//
LOCUS       DAOSOW010000035         7671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_9536_length_7671_cov_3.945378, whole genome shotgun sequence.
ACCESSION   DAOSOW010000035 DAOSOW010000000
VERSION     DAOSOW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9536_length_7671_cov_3.945378"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1833
                     /locus_tag="PLD67_01725"
     CDS             <1..1833
                     /locus_tag="PLD67_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT21277.1"
                     /translation="VSHTIGALNVGNNYEIIEKKGSKGTFLIPKNYLDKNKEMFNIGS
                     DGILKIPEGYFFFQEKGIVQPQSAAVIIDYKTGKIKAMIGGRQIEGSKTFNRAVDATR
                     QPGSTIKPLSVYLAALDLGYSAAYPIDDLPKYNQSGERWPKNWYEHRNIKYWGIQTLR
                     RSIEQSINTNAVTMLETIGLDAAINSLSRLRLIDQNNPDKDSFVSPQEDPYYNDVNLA
                     SLALGGLTKGFSPLAMTAAYGAIANDGVYVEPIVYTRVVNTKGETILENIPETHVAYS
                     PEVASLMKDILRTTVNPGLSYRARLPQELGIEVAGKTGTTQANGDFWYVGFSPYYVGG
                     IWVGNDNVQMKLSGDSGNNARLWSAIMTPIHQGLPPATFTRNPNLIPISVCKVSGKLP
                     GDLCALDQRGSQIITEYFIPGTQPTETCDVHVKAEVCTSSNMKKSIYCPGNLVEERVF
                     FTRDPLFDPEEKTDNYEAKKLYQQILEDRIIFTMEELQQIYAGQMSVDENYQITHVLG
                     IEVEKLGPSGFITEDYQYQVPTRTCYYHTKWHYEQWLNEGDGNGNNGNGNGNNGNGHN
                     GNGNGNNGNGDTLEDIINQIIDEFENSEEDPDKPIDEILESIEN"
     assembly_gap    2123..2222
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2224..2580)
                     /gene="spoVAE"
                     /locus_tag="PLD67_01730"
     CDS             complement(2224..2580)
                     /gene="spoVAE"
                     /locus_tag="PLD67_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HOT21278.1"
                     /translation="MNYLMSFVIGGLICVVGQIIIDTFKKNNAYLLVFFVVTGAILGF
                     FGVYDKLVEIGHSGATVPLLGFGNSLAKGAMEEAANKGFMGALSGGVIKTAPGVAAAL
                     LFGYIVAVIFNPHGDK"
     gene            complement(2589..3581)
                     /gene="spoVAD"
                     /locus_tag="PLD67_01735"
     CDS             complement(2589..3581)
                     /gene="spoVAD"
                     /locus_tag="PLD67_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HOT21279.1"
                     /translation="MKKTGKTYIIKDDVVIRDTYTIVGKKEGQGPLKDKFDMVIEDDL
                     WGEDTWEKCELKMQKIAVENLKNKNSLINENIDLLISGDLLNQITSSSFAARALELPY
                     IGVYGACSTMTLTMGLGAMIIDGGFAENVICVTSSHFCSAERQYRLPLEMGGQRHMSA
                     QWTVTGAAAVWLSKSGKGPKIKHVTFGKIVDLGVKDANNMGAAMAPAAYDSLNQHLQD
                     TGIDYDLIVTGDLGTVGKGIVNKMFNEDNKSIESKYDDCGTIIFDINKQDVHAGGSGC
                     GCSAAVFGSFLYKKLLNNEIKKLLFTATGALHSPTANLQGESIPGIAHCLGIEV"
     gene            complement(3591..4031)
                     /gene="spoVAC"
                     /locus_tag="PLD67_01740"
     CDS             complement(3591..4031)
                     /gene="spoVAC"
                     /locus_tag="PLD67_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008640130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HOT21280.1"
                     /translation="MKYNKDNYKQVVDEFTPKNKMLKNCVMAFIFGGAICLVGEIIND
                     SFVAWLGISDEEASPMTAVVLIGISALLTGLGWYDKLGKIGGAGTVVPITGFANSVVS
                     PALEFKREGLILGTAANIFKLAGPVLVFGYFSSFIAGLLSMLFK"
     gene            complement(4343..4819)
                     /gene="spoIIAB"
                     /locus_tag="PLD67_01745"
     CDS             complement(4343..4819)
                     /gene="spoIIAB"
                     /locus_tag="PLD67_01745"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020154489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HOT21281.1"
                     /translation="MHNNYVYIKIPSLSVNESFARAAVAAFCSSLNPTMSEISDIKTA
                     VSEAVTNAVVHAYEDEIGEIEIQCRIKGQVVEIIVEDFGCGIADVEKAREPLFTTRPE
                     LERSGMGFSVMESFMDELVVLSEKDVGTKVILRKKISEKKQKTLNEKFDDEKSEDD"
     gene            complement(4829..5134)
                     /locus_tag="PLD67_01750"
     CDS             complement(4829..5134)
                     /locus_tag="PLD67_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451156.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HOT21282.1"
                     /translation="MNIQFIETGQVLTIKLKGELDHHSADEARVLIDEKIKNEKYNKL
                     VIDLKGLDFIDSSGIGFVIGRYKVIRKRNGVIEIINAGKKVRKILDMSGIGKIIKIK"
     gene            complement(5237..6649)
                     /locus_tag="PLD67_01755"
     CDS             complement(5237..6649)
                     /locus_tag="PLD67_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT21283.1"
                     /translation="MVKTILRKLLNLILYPFRITVKLLISLLKRLKFSIAFKISTTYL
                     ILYVIIIMTVALTTSVGYLIFELQKFEDTTAAIDSENIINSYPNFNTINVNERSYNSF
                     EIFDNNLNTYFTTGRPGRYADSIIVITEKIIFDNEYVHSESFTKNNSIYYMNINYPME
                     DMLRDAWRISFMVLISGAIGLMFFAPIVSSTSYKLIGPIKNMTEITKTITVNNINTRL
                     DVKGTQDELKELSQTFNEMMDRIESGYKTQQQFVSDASHELRTPIAVIKGYVNMLDRW
                     GKDDKAVLEESIVAIKNETENMQDLIEKLLFIARSDISTLNFTKEDFRIRALLEEIEK
                     ETKMIDTAHKLYFKFYDDATIYGDKMRIKQAVRILLDNAMKYTPPGEYIMISGFIQDE
                     YYVIKVEDTGVGIKKDDLNKIFDRLYRAEQSRSKQVSGHGLGLSIAKIIVLGHKGKIK
                     VKSTPGKGSEFKLLFPYIDS"
     gene            complement(6660..7340)
                     /locus_tag="PLD67_01760"
     CDS             complement(6660..7340)
                     /locus_tag="PLD67_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT21284.1"
                     /translation="MGKKKILIVEDEQKIARFLELELKYEGYDVDIANNGREGFEKGK
                     SPDVDLIILDLMLPGLSGIEVCRRLRQTSDVPIIMLTAKDDISDKVTGLDIGADDYMT
                     KPFAVEELLARIRVLLKRKYHKTNEQDILKIGKLTLCKNNYKVEFDNENIELTKKEFE
                     LLEYLMKNKNIVISREKILDNVWGYDYYGDTNIIDVYIRYLRSKIDQKYNVSLIETVR
                     GVGYIIRD"
     gene            complement(7478..>7671)
                     /locus_tag="PLD67_01765"
     CDS             complement(7478..>7671)
                     /locus_tag="PLD67_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21285.1"
                     /translation="VLAILTSCKITVYRDKAINVNNIKKNMQETVEKIKDKSEEIFKD
                     DEEEPTDEQDESENEIIIE"
BASE COUNT         2280 a         1494 c         1187 g         2610 t
ORIGIN      
        1 gtttctcaca ccatcggagc tctaaatgtg ggaaataatt atgaaataat tgagaaaaaa
       61 ggctcaaaag ggaccttttt gatacctaaa aactatttgg ataaaaataa ggaaatgttc
      121 aatataggaa gtgacggtat tttaaaaata ccggaaggat atttcttctt ccaggaaaaa
      181 ggtattgtac agcctcaatc agcagcagta ataatcgatt acaaaacagg aaaaattaaa
      241 gcaatgattg gcggaaggca gattgaagga agcaaaacct ttaatcgtgc tgtagatgcg
      301 acaagacagc cgggctcgac aatcaaaccg ttatcggtat atttggcggc tcttgattta
      361 ggatattcag ctgcttaccc aattgatgac ctcccaaaat acaatcaaag cggtgaaaga
      421 tggcctaaaa actggtatga acacagaaat ataaaatatt ggggtataca aacccttaga
      481 aggtcgatag agcagtcaat aaatacaaat gctgttacca tgcttgaaac aatcggactt
      541 gatgctgcta taaactcatt atcaagatta agactaatcg accaaaataa ccctgacaaa
      601 gattcctttg tatctccgca ggaagatcct tattacaatg acgtgaactt ggcatctctt
      661 gctttgggcg gacttaccaa aggattttct ccattggcaa tgactgctgc atacggcgcg
      721 attgccaatg atggagtata tgtagagccg atagtatata cgcgagttgt taatactaag
      781 ggtgaaacaa tactggaaaa tatccctgaa acacatgtgg catacagtcc tgaagtcgct
      841 tcgctgatga aggatatttt aagaacaaca gtaaatcccg gactttcata tagggcaaga
      901 cttccccagg aattaggtat cgaagttgca ggaaaaacag gtacaacaca ggcaaacgga
      961 gatttctggt atgtcggttt ttcaccatac tatgtaggag gaatttgggt tggtaatgat
     1021 aatgttcaaa tgaagctttc cggagacagc ggcaacaacg caagactttg gagtgcaatt
     1081 atgactccga tacatcaagg tctgcctcct gcaacattta cacgaaatcc aaacctaata
     1141 ccaattagtg tttgtaaagt atcaggaaag ctgccgggtg acctttgtgc acttgaccag
     1201 agaggaagtc agattattac ggaatatttt atacccggaa ctcaacctac tgaaacctgc
     1261 gacgtacatg ttaaagctga agtatgtacc agctcaaata tgaaaaaatc catatactgt
     1321 ccgggtaatt tggtggaaga aagagtgttc tttacgagag acccgctttt tgaccctgaa
     1381 gaaaagacag ataactatga agcaaaaaaa ttatatcagc aaattttaga agacagaatt
     1441 attttcacta tggaagaact tcagcaaata tatgccggtc agatgtcagt tgatgaaaat
     1501 tatcaaataa ctcatgtttt aggaattgaa gttgaaaagt taggtccttc cggatttata
     1561 accgaagact atcagtatca ggttccaaca aggacttgtt attatcacac caaatggcat
     1621 tatgaacagt ggttgaatga aggcgacgga aacggtaata atggtaatgg aaacggtaat
     1681 aacggcaatg gccataatgg aaacggcaac ggcaataacg gcaatggtga tacattggaa
     1741 gatattataa atcagataat cgatgaattt gagaattcgg aagaagaccc tgataaaccg
     1801 atagatgaaa tattggaatc catagaaaat taagcagacg acccaaatct ttgatttggt
     1861 gtcggtcgct tatcctgagc aaagcgaagg atctcatacg tcatcctgag caaagcgaag
     1921 gatctcatga gattcttcgg gctaaagccc tcagaatgac aaaaatgtta tttatagtga
     1981 taacgagggc tatgccttct tattgaaagt aattgataat aatttgtaag aaatataaaa
     2041 gagttcagaa tttcgattct gaactctttt atttatcacc atgaggattg aatataacag
     2101 caacgatata tccgaataac aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nntttattta tcaccatgag gattgaatat aacagcaacg atatatccga ataacaatgc
     2281 tgcagcaact ccgggtgctg tttttatcac tcctccgctc aatgctccca taaacccttt
     2341 atttgcggct tcttccatag ctcccttggc taaggagttg ccaaagccga gaagaggaac
     2401 ggtagctccc gaatgcccta tttctacaag cttatcataa actccgaaga atcccaatat
     2461 tgcacctgtc acaacgaaaa acacaagcag ataagcatta ttctttttga atgtatcgat
     2521 tattatctgg ccgactacac atatcaaacc gcctattaca aaactcatca aataattcat
     2581 atgccctcct aaacttcaat acccaagcag tgagcgattc cgggtatact ttcaccctga
     2641 agatttgcag taggcgaatg aagtgctccc gtagctgtaa acagaagttt ttttatttca
     2701 ttattcaata atttcttgta taaaaaggag ccgaatactg ctgctgaaca tccgcagccg
     2761 cttcctcccg catgtacatc ctgtttgtta atatcaaaaa taattgtacc gcagtcatcg
     2821 tattttgatt cgatactctt attatcttca ttgaacatct tattaactat gcctttaccc
     2881 actgttccca aatctccggt aacaattaaa tcataatcaa tgcctgtatc ctgaagatgc
     2941 tgatttaatg agtcgtaagc agccggtgcc atggcagctc ccatattatt tgcatctttt
     3001 acacctaaat caacgatttt tccaaaggta acatgtttta tttttggtcc ctttccgctt
     3061 tttgagagcc ataccgcagc tgcgccggtt actgtccact gtgctgacat atggcgctgt
     3121 cctcccattt caagcggaag tctgtactgc ctttctgcac tgcaaaaatg gcttgatgta
     3181 acacaaatta cattttcagc aaatcctccg tctattatca ttgcgcctaa acccattgtc
     3241 aatgtcatgg tggagcaggc gccgtatact cctatgtatg gcagttccaa ggctcttgca
     3301 gcaaatgaag aagatgtgat ttgatttaac aaatctcctg aaattaaaag gtcaatgttt
     3361 tcatttatta aactattttt gtttttcaaa ttttctactg caattttctg catttttaat
     3421 tcacattttt cccatgtgtc ttctccccat aaatcatctt ctattaccat atcaaattta
     3481 tcttttaaag gtccctgtcc ttcctttttg ccgactatgg tgtaagtatc tcttattact
     3541 acatcgtctt taattatata agtttttcct gtctttttca tttttcctcc ttacttaaat
     3601 aacatactta acaaacctgc tataaatgaa gaaaagtacc cgaataccaa gacaggtcct
     3661 gctaacttaa aaatatttgc agccgtacct aaaatcaatc cttctctctt aaattccaag
     3721 gcaggagaaa ctaccgaatt tgcaaatccg gttattggaa cgactgtacc ggctcctcct
     3781 attttgccta gtttatcata ccagcccaaa cccgtcaaaa gtgcactaat acctattagt
     3841 accacagctg tcatgggtga tgcttcttca tctgatatac ctaaccaggc aacaaaagag
     3901 tcattgataa tttctcccac caaacatatg gctcccccaa aaataaatgc catcacacag
     3961 tttttaagca ttttgttttt aggtgtaaac tcatcaacta cttgtttata gttgtcttta
     4021 ttatatttca ttttacctcc taatgtataa ttaaaggtat tacagtaaga ctgcgctttc
     4081 aggtattctt ttcagggatc tgtcaattcc gctgccgaaa taaacagctg ttaagccgat
     4141 aattaaaagt attactaata aaaatatctt ttttttcata taaatcacct cataagtatt
     4201 atttacaaaa atagtttttt gaatactaaa aaaaagcact aacagtgcct tttttttaaa
     4261 gaaatgttaa atagtacctt ttgagtcaca tagatattta tatttgttca agataaaaat
     4321 ttctatttcg cttaattatt tattaatcat cttctgattt ttcatcatca aatttttcat
     4381 ttaatgtctt ttgttttttt tcagatattt tttttcttaa tataactttt gttccaacat
     4441 ccttttctga caaaacaacc aattcatcca taaatgattc cataacggaa aaacccattc
     4501 cggaacgttc aagctccggt cttgtggtaa ataacggctc tcttgccttt tctacatctg
     4561 caattccgca gccgaaatcc tcaactatta tttcaactac ctgcccctta attctgcatt
     4621 gaatttcaat ttctccgatt tcatcttcat aggcatgtac cactgcattg gttactgctt
     4681 cggacactgc tgtcttaata tctgatattt cgctcattgt tggattcaag cttgagcaaa
     4741 atgctgcgac cgcagccctt gcaaaggatt cattaacaga tagacttggt attttaatat
     4801 atacgtagtt attatgcatt ttgtccccct attttatttt tattattttt ccaatgccgc
     4861 tcatgtccaa aatctttcgt acctttttgc ctgcatttat tatttctatt acaccattac
     4921 gctttcttat gactttgtat cgaccgatta caaagccgat acctgagctg tcgataaaat
     4981 caagaccttt taaatctatt accaatttat tgtacttttc atttttgatt ttttcatcaa
     5041 ttaataccct agcttcgtcg gcgctgtgat ggtctaactc gccctttaat ttgattgtca
     5101 atacttgtcc cgtttcaata aactgaatat tcacaaaatc atcctttctg cataatgtac
     5161 aagctattat actactaggg aaaggatatt tccttgactt attttaatgt tttttgtcga
     5221 ttatggtaaa tttaaatcaa ctgtcaatat aaggaaaaag taatttaaat tcggaacctt
     5281 ttccgggtgt gcttttaacc tttattttac ccttatgacc taatacgatt attttagcaa
     5341 ttgacaaccc cagtccatga ccgcttactt gcttgcttct tgattgctct gccctataaa
     5401 gtctgtcaaa tattttattt aaatcatctt ttttaattcc tacaccggtg tcctctactt
     5461 ttataacata gtattcatcc tggatgaagc ctgaaatcat aatatattcg ccgggaggag
     5521 tatatttcat ggcattatcg agaagtattc tcacagcctg tttaatcctc attttatcgc
     5581 cgtaaatggt tgcatcatca taaaatttaa aatacagttt gtgcgctgtg tcaatcatct
     5641 ttgtttcttt ttcaatttct tcaagaaggg cacgaattct aaaatcttcc tttgtaaaat
     5701 tcaaagtgct tatatcgctt cttgcaataa ataataattt ttcaattaaa tcttgcatat
     5761 tttcggtttc attttttatt gctacgatag attcttcaag cactgcttta tcatctttgc
     5821 cccatctgtc aagcatatta acatagccct ttataacagc tatcggtgtc ctgagttcat
     5881 gggatgcatc tgaaacaaac tgctgctgtg ttttatatcc gctttctatc ctatccatca
     5941 tttcattgaa ggtttgcgac aattccttca attcatcctg tgttcctttc acgtcgagcc
     6001 tggtgtttat gttgtttact gtgatagtct tggtaatttc agtcatgttt tttatgggac
     6061 cgatgagttt gtaacttgtg cttgatacta ttggtgcgaa aaacataaga ccaatggctc
     6121 cggatattaa aaccataaaa cttattctcc acgcatccct gagcatatct tccatcgggt
     6181 aatttatatt catataataa atagaattgt tttttgtaaa gctttccgaa tgaacatatt
     6241 cattgtcaaa aattattttt tccgtaatga caataatgct gtctgcatat ctacccggtc
     6301 ttcctgtcgt aaagtatgtg ttaagattat tgtcaaatat ttcaaaagaa ttatagcttc
     6361 tttcatttac atttatagtg ttaaaattcg ggtaactgtt tattatattt tctgaatcga
     6421 ttgctgctgt tgtatcttca aatttctgca gttcaaatat taaatagcct acagaggttg
     6481 taagggcaac tgtcataatt attataacgt acaagattaa atatgttgta gaaattttaa
     6541 atgcaattga aaatttaagc ctttttaaca gacttattag caattttacg gttattctaa
     6601 atgggtaaag tattaagttt aacagttttc taagaattgt ctttaccatt tgtcttcctt
     6661 caatctctga ttatgtagcc tactcccctg acagtttcaa taagacttac attatatttc
     6721 tggtctatct tgcttcttaa atatcttata tatacatcaa taatatttgt gtctccataa
     6781 taatcatatc cccaaacatt atccaaaatc ttttctcttg atattactat gtttttgttt
     6841 ttcataaggt attccaataa ttcaaattct tttttggtaa gctcaatatt ttcattgtcg
     6901 aattcaacct tataattatt tttgcataat gtaagtttcc cgattttcaa tatatcttgt
     6961 tcattggttt tatggtattt tctttttaac aatactctga tgcgcgccaa taattcttct
     7021 acggcaaagg gctttgtcat ataatcgtcg gcacctatgt ccagccctgt tactttatca
     7081 gaaatatcat cctttgcagt cagcattatt atgggtacat cagatgtttg tcttaatctt
     7141 ctgcacactt caatgccgct taatccgggc agcattaaat caagtattat caaatcaaca
     7201 tcaggggatt tccctttttc aaatccttct cttccgttgt ttgcaatatc tacatcatag
     7261 ccctcgtatt ttaattcaag ctccaaaaac cgcgcaatct tttgctcatc ctccacaatt
     7321 aaaatctttt ttttacccat aataacctcg tttttgattt attgtactat ttttttcctt
     7381 ttttatcaat cagtttttac tattcttata tcttctctat cgcgagtaca ttatgtcatt
     7441 aatgaataat atcaggccta agacctgata ttatttttta ttcaatgatt atttcatttt
     7501 ctgattcatc ttgttcgtct gttggctctt cttcgtcatc cttaaagatt tcttctgact
     7561 tgtcttttat tttttccacc gtttcctgca tatttttctt tatattgttt acatttattg
     7621 ctttatccct gtaaacagtt attttgcagg atgtcaatat tgccagtacc a
//
LOCUS       DAOSOW010000036         7660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_9559_length_7660_cov_3.416042, whole genome shotgun sequence.
ACCESSION   DAOSOW010000036 DAOSOW010000000
VERSION     DAOSOW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7660
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9559_length_7660_cov_3.416042"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /gene="truB"
                     /locus_tag="PLD67_01770"
     CDS             complement(<1..619)
                     /gene="truB"
                     /locus_tag="PLD67_01770"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOT21286.1"
                     /translation="MIGILNILKPTGMTSHDVVSNVRKILNIKKVGHAGTLDPNVAGV
                     LVICVGKATKFSEYLMSGDKEYVCELILGQSTDTQDSYGMVIDKSDKVSVTREELNEV
                     LKKFTGELIQIPPAYSAVKLHGKKLYEYARQNIQVEKQGRPINIKSIELLKFDGNKAL
                     LKVRCSKGTYIRTLCNDIGNSLGTFGHMGILIRTESKGLHINNSVT"
     gene            complement(619..1581)
                     /locus_tag="PLD67_01775"
     CDS             complement(619..1581)
                     /locus_tag="PLD67_01775"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOT21287.1"
                     /translation="MLKNDVKEIFNLINNSDKICIAGHKAPDGDCIGSVMALYNFLKP
                     LNKNITVLMDGNIPYNYKAFIDEKIISNSYNNEEYDLLIVLDTSDRKRLGKFENVLNN
                     SKKTVVIDHHKTNEGFGNINIIDTEMSSTGELVYDILKAADADISLEVAALIYLAILT
                     DTGKFSYRNTTSNTHRKAAELIDIGVNVSEIDNIVYNSKPSNIVRAFMECVSGVELYY
                     DNKLGITAITKNILNKHNADMADIDGVVEYIREIKEVEVSCVLKENDEGTKVSLRSKN
                     NIDVAAVSVKYKGGGHAKAAGFDINENIESTKKIIIEEFKKYFA"
     gene            complement(1568..1945)
                     /gene="rbfA"
                     /locus_tag="PLD67_01780"
     CDS             complement(1568..1945)
                     /gene="rbfA"
                     /locus_tag="PLD67_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOT21288.1"
                     /translation="MGNRRNNRLSGEVRKAISEIIINDVKDPRISELMSITDVHVTED
                     LKYAKVFVSDYNNIETTLTALESAKGFIRKELGKKVKMRIIPELIFIKDDSIERGLHM
                     SSLIDKVIEEDKTRKVNKDNAEE"
     gene            complement(1959..4157)
                     /gene="infB"
                     /locus_tag="PLD67_01785"
     CDS             complement(1959..4157)
                     /gene="infB"
                     /locus_tag="PLD67_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOT21289.1"
                     /translation="MRIYELAKELEMNSKDLTQLVEQELGITVKNHMSSLTDLEVLRF
                     KKALSLLNNSKKTKLVKSSKNVIVDEEPLVDEKIDLNQAKLKKVDKKLLSPVKKHTPT
                     KTGPAKLHHDEEEKKEIRKDIKKDKQKQKPQQQQQSRPEPKQHKVKNISIEDSIVVRD
                     FADKLGVPVNVVISKLIMLGVMANINQEIDYDTASLIGEELGAKVNKIEVVDELKNLE
                     DSLFKDDKDDESNLQPRPPIVTVMGHVDHGKTSLLDAIRNTSVTEHEAGGITQHIGAS
                     QVEINGKKITFLDTPGHEAFTAMRARGAKVTDIAIIVVAADDGVMPQTIEAISHSKAA
                     GVPIIVAVNKIDKPTANPDKVKQELSEHGVISEDWGGDTIFVHVSAKKGTGIEELLEM
                     ILLVAEVQELKANPNRNAKGTIIESKLDKGRGPVATVLIEKGTLKKGDYVLVGSTHGK
                     VRAMFNSKGKRINQAGPSVPVEILGLSETPQAGDILVVMENEKDAKNIAEKRKDKKHL
                     ESINVSSKVSLDDLYERIQKGEVKDLNIIIKADVSGSIEAVKQSLQKLSVDEVKVNPI
                     HGGVGGINENDILLASASNAIVIGFNVRPSVAALELAKQENVDVRTYNIIYNAIEDIE
                     SAVKGMLKPVFKEVVMGRAEVRQTFKVPNVGIVAGVYVTSGKITRKSRIRLLRNDIVI
                     HDGQIASLKRFKDDVSELSSGYEGGIGLDKYNDIKIGDSMEAYIMEEIKR"
     gene            complement(4173..4466)
                     /locus_tag="PLD67_01790"
     CDS             complement(4173..4466)
                     /locus_tag="PLD67_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOT21290.1"
                     /translation="MHKIYSMLGIARKGGNVSIGFDSTKADIEKNNSFLVIIAEDASD
                     KTKKNIKFVCDKYNCEYIETGEKELLGKALGKKVVSVLSIRDRNIASYLLNNV"
     gene            complement(4459..4728)
                     /locus_tag="PLD67_01795"
     CDS             complement(4459..4728)
                     /locus_tag="PLD67_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HOT21291.1"
                     /translation="MKVRKIPMRRCIGCMESFPKKELCRIVKTKENEIFFDETGKKNG
                     RGTYICKNIECFNKAIKSKRLAKSLETEIPEEVIEAIKEAIEKNA"
     gene            complement(4725..5783)
                     /gene="nusA"
                     /locus_tag="PLD67_01800"
     CDS             complement(4725..5783)
                     /gene="nusA"
                     /locus_tag="PLD67_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOT21292.1"
                     /translation="MNKDILNALSELEREKGILKDVVLEAIRSALETGYKKNYGKATN
                     FSIEIDKTTGEYKLFQDKTVVEKVENKNLEISLEEAKSIDSKYELGDIVKIQIEPKED
                     FGRIAAQTARQVILQKIREAERESIFSEFYGRESDILTGIVQRENKGNIFVDLGKIEG
                     ILSVNEQIPGEVYNPGDRIKTVILEVKNTGKGANIMLSRSHPNLVKRLFELEVPEIHD
                     GIIEIYSIAREAGSRTKIAVFSHDPNVEPIGSCVGNKGTRVKNIIDELNQEKIDIIIY
                     EKDPEKFIANSLGPAKILKVTANEKEKTAVTVVPDNQLSLAIGKEGQNVRLAAKLTGW
                     KIDIISESQYNDSLREEV"
     gene            complement(5797..6261)
                     /gene="rimP"
                     /locus_tag="PLD67_01805"
     CDS             complement(5797..6261)
                     /gene="rimP"
                     /locus_tag="PLD67_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOT21293.1"
                     /translation="MNKKDIVSSVRKITEEILKSTDIELFDIEYVKEGPFKYLKVYID
                     KPGGITVDDTADISRVLNKKLDEVDLIKEQYFLEVSSPGVERPFKTEKDYLKNLNKLV
                     EARLYKPIDDKKTVKGILTEKNENNIVIKSGEDIFTIELKDLAKINRVIEDF"
     gene            complement(6544..>7660)
                     /gene="polC"
                     /locus_tag="PLD67_01810"
     CDS             complement(6544..>7660)
                     /gene="polC"
                     /locus_tag="PLD67_01810"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582699.1"
                     /note="catalyzes DNA-template-directed extension of the
                     3'- end of a DNA strand by one nucleotide at a time;
                     required for leading strand synthesis; PolC exhibits 3' to
                     5' exonuclease activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HOT21294.1"
                     /translation="EKSGTITTHFDYHSISGRILKLDLLGHDTPSIIRMLEDLTGLES
                     KDIPMDDPSTMAIFNSPEILGISLDEINCSTGTLAIPEFGTSFVRQMIKDTKPKSFSD
                     LVRISGLSHGTDVWINNAQDIIKKGYAKIGGVISTRDDIMTYLIQIGVEKKTAFDIME
                     RVRKGKGLRKEDEEAMKAQNVPQWYIDSCNTIKYMFPKAHAVAYVTMSVKIAYYKVHY
                     PEAFYATYFTTKVDDFDADIVIKGQNHILNTIKSLEEKGNDKTAKEKNLITVLEVAYE
                     MYKRGFKFTKVDLYKSDDRKFLLTEDGILPPLMGLQGLGANAAANIKAEREISEFISI
                     EDIINRAKVSKTVIEVLEKHGCLKNMDETNQISIFNV"
BASE COUNT         2004 a         1637 c         1040 g         2979 t
ORIGIN      
        1 gggtcacaga attattaata tgtaaacctt ttgactctgt cctgattaaa attcccatat
       61 gtccgaatgt acccaggctg tttccaatat cattgcataa agttctgata tatgttccct
      121 tgctgcatct gacttttaaa agagctttat tcccgtcaaa tttaagaagt tcaatacttt
      181 taatatttat cggtcttcct tgtttctcta cttgtatatt ttgccttgca tattcataca
      241 gttttttacc atgtagttta actgctgaat atgcaggagg aatttgaatt agctctcccg
      301 taaatttttt taaaacctca tttaactctt ctcttgttac tgatacttta tcagatttat
      361 caataaccat cccgtagctg tcctgagtat cggtgctttg tcccaatatc agctcacata
      421 catattcttt gtcgccgctc attaagtatt cgctgaattt tgttgcttta ccgacacaaa
      481 taaccaatac tcctgccaca ttagggtcaa gagttcctgc atggcctacc ttttttatat
      541 ttaatatttt tctgacatta cttacaacat catgggatgt catgcctgtt ggttttaata
      601 tatttaatat tcctatcatt acgcaaaata ctttttaaat tcctcaatta ttattttttt
      661 tgtactttct atgttttcat taatatcaaa tcctgcggcc tttgcatgac cgccgccttt
      721 gtatttaact gaaacagcag caacatctat attgtttttt gaccttagac tcacctttgt
      781 accttcatcg ttttctttaa gtacgcagga tacttcaact tcttttattt ctctgatata
      841 ttcaacaact ccgtcaatat cagccatatc ggcattgtgt ttattaagta tattttttgt
      901 tatagccgtt atgcctaatt tgttgtcata ataaagctca accccgctta cgcactccat
      961 aaatgctctt actatattgg agggtttgga attataaaca atattgtcta tttccgatac
     1021 attaacaccg atatcaataa gctctgcagc tttcctgtgt gtattgctgg tagtgtttct
     1081 ataagaaaac tttcctgtgt cggttaatat ggcaaggtag attaaagcag caacttcaag
     1141 agaaatatca gcatctgcag cttttaatat atcatatacc aattctcccg tggagctcat
     1201 ttctgtatct attatattta tattaccgaa gccttcattg gttttgtggt ggtcaataac
     1261 tactgttttc tttgagttgt ttaacacatt ttcaaatttg cccagccttt ttctgtcact
     1321 tgtgtccaat acgatcaaaa ggtcatattc ttcattgtta tagctgtttg aaattatttt
     1381 ttcatctata aaggctttat aattatatgg aatattgccg tccatcaata ccgtgatgtt
     1441 tttatttaat ggtttaagaa agttataaag ggccataact gaccctatgc agtctccatc
     1501 gggcgcctta tgtcctgcta tacaaatttt gtcagaatta tttattaaat taaatatttc
     1561 cttcacatca ttcttcagca ttatctttat taacctttct tgttttatct tcttcaataa
     1621 ctttgtcgat aagactgctc atgtgcaagc ctctttcaat tgaatcatct ttgataaata
     1681 tcagctcggg aataattctc attttaactt tcttacccag ttcttttctt ataaaaccct
     1741 tggcactttc caaagcagtt aaagtagttt caatattgtt atagtctgaa acgaatactt
     1801 ttgcatattt caaatcttct gttacatgta catccgttat actcataagc tctgaaattc
     1861 tcgggtcctt tacatcattt atgattattt cggatattgc ttttctaact tcgcccgata
     1921 acctgttgtt tcttctatta cccatttctt acctcttttt atctttttat ttcttccatt
     1981 atataggctt ccattgaatc acctatttta atgtcattat atttgtcaag acctattccg
     2041 ccttcataac cgctgctaag ttcagacaca tcatctttaa atcttttcag cgatgcaatt
     2101 tgtccgtcat gtattactat atcattcctc agcaatctta ttctcgattt tcttgtaatc
     2161 tttccgcttg ttacataaac cccggcaaca atccccacat ttggcacctt gaatgtctgt
     2221 cttacttcag ctcttcccat aactacttcc ttgaatacag gtttaagcat tcccttaaca
     2281 gcagactcaa tatcttcaat tgcattgtaa ataatattgt aagtacgtac atccacattt
     2341 tcctgttttg caagctcaag agctgcaacc gaaggtctta cgttaaatcc tattactatt
     2401 gcatttgaag cagaagcaag caaaatatca ttttcgttta ttccacccac accaccgtgt
     2461 ataggattaa cttttacttc gtccacgctc aatttttgca atgattgttt aactgcttct
     2521 attgagccgc ttacatccgc ttttataatg atatttaaat cttttacttc acccttttga
     2581 attctttcat ataagtcatc gagtgacacc tttgatgaaa cattgatact ttcgaggtgc
     2641 tttttatcct tgcgtttttc tgcaatattt ttagcatcct tttcattttc cattactacc
     2701 aatatatctc ccgcttgagg cgtttcagaa agtcctaata tttcaaccgg aacagacggt
     2761 cccgcctgat taattctttt gccttttgaa ttgaacatgg ctctgacctt gccatgagtg
     2821 cttcctacca atacataatc gccttttttc aatgttccct tttcaatcaa tacggtagca
     2881 acaggacctc ttcctttatc aagctttgat tcaataattg taccctttgc gtttctgttt
     2941 ggattagctt ttaattcctg aacttctgca accaatagta tcatttccaa cagttcttct
     3001 ataccggtgc ctttttttgc cgaaacatgc acaaatatag tatctccgcc ccaatcctcg
     3061 gatataacac cgtgctctga cagctcctgc tttactttat ccgggtttgc agtaggttta
     3121 tcgattttat ttacggctac aattatagga actcctgcag cttttgaatg gcttattgct
     3181 tcaattgttt gaggcattac accgtcatct gcagctacta caattatcgc tatatcagtg
     3241 acttttgctc cccttgccct cattgcggta aatgcttcat gtccgggagt atcaaggaat
     3301 gttatttttt tgccgttaat ttcaacctgt gacgctccta tatgctgagt tattcctccg
     3361 gcttcatgct ctgtaacaga tgtattcctg attgcatcaa gaagcgaagt ttttccatgg
     3421 tcaacatgac ccattacagt aactataggt ggtctcggct gcaagttact ttcatcatct
     3481 ttatcatctt taaataatga atcctctaaa tttttaagct catcaacaac ttctatttta
     3541 tttacttttg cgcctaattc ttctccaatc aatgatgcgg tgtcatagtc gatttcctga
     3601 tttatatttg ccattacacc gagcataatc aacttggata ttacaacatt tacgggaaca
     3661 cccaacttgt ctgcaaaatc tcttactaca atagaatcct caatgctgat atttttaacc
     3721 ttgtgttgtt tcggctcagg tcggctttgc tgctgctgct gtggtttctg tttttgctta
     3781 tcctttttta tatccttcct aatctctttt ttctcttctt catcatgatg taattttgca
     3841 ggtcccgtct tagtcggagt atgcttttta accggtgata acaatttttt atcaaccttt
     3901 ttaagctttg cttgattcaa atctattttt tcgtcaacca agggctcttc atctacgata
     3961 acatttttgc tgcttttgac aagctttgtt tttttactgt tattaagaag gcttaatgcc
     4021 tttttaaatc ttaaaacctc taaatcagtc agggagctca tgtgattttt cactgtaatt
     4081 cctaattcct gttcaactaa ttgtgtaaga tctttgctgt tcatttctaa ttcttttgca
     4141 agttcatata ttctcatgca accacctccg gtttatacat tatttaataa atacgatgct
     4201 atgttcctgt ctcttattga cagaacactc accacctttt ttccaagagc ctttcccaat
     4261 agttcttttt cacccgtctc aatatattcg caattatact tatcacatac aaatttaatg
     4321 tttttcttag ttttatctga tgcatcttct gcaattataa ccaaaaaact attgtttttt
     4381 tctatatccg ccttcgttga atcaaaacct atggatacat ttcctccttt tcttgcaata
     4441 cctaacattg agtatatttt atgcattttt ttcaatcgcc tcttttattg cttctatcac
     4501 ttcttcagga atttcggttt ccaatgattt tgccaacctc tttgatttaa ttgccttatt
     4561 gaagcattct atatttttgc atatataagt tcctcgtccg ttcttctttc ctgtttcatc
     4621 gaaaaaaatt tcattttctt ttgtttttac aattctgcat aattcttttt tggggaaact
     4681 ttccatgcag ccgatacatc ttctcattgg tattttcctg actttcatac ttcctcccgt
     4741 aagctgtcgt tatactgaga ttcgcttatt atatcaattt tccaccctgt aagctttgca
     4801 gcaagcctta cattttgtcc ttcctttcca attgccaaag aaagttgatt gtcgggaaca
     4861 acagtcactg ccgttttttc cttttcattt gctgtaactt taagaatttt ggcaggtccc
     4921 agactattgg caataaattt ttcgggatct ttttcataaa taataatatc aattttttcc
     4981 tgatttaatt catcaataat atttttaacc cttgtacctt tatttcctac acaggagcct
     5041 atgggctcaa cattggggtc atgggaaaaa actgctattt ttgttcttga gccggcttct
     5101 cttgcaattg agtatatttc aataatgcca tcatgaattt caggaacttc aagctcaaat
     5161 aacctcttga caagatttgg atggcttctt gacagcataa tgtttgcacc ttttcccgta
     5221 ttttttacct ccagaataac agtctttatc ctgtcacccg gattatatac ttcgcccggt
     5281 atttgctcat ttacggatag tattccttca attttgccta aatccacaaa tatgttaccc
     5341 ttgttttctc tttgtactat tcctgtcaaa atatctgatt ccctgccgta aaattcactg
     5401 aaaatagatt ctctttcagc ttcgcggatt ttttgcaaaa ttacctgtct tgctgtttga
     5461 gctgcaattc gaccgaaatc ctctttgggc tcaatttgta ttttgactat atctcccaat
     5521 tcatattttg aatcgattga ttttgcctct tccaatgaaa tttccaaatt tttattttct
     5581 actttttcta caacggtttt gtcctgaaaa agtttatatt cacctgttgt tttatctatt
     5641 tcaatgctga agtttgttgc cttaccgtaa ttcttcttat atcctgtttc caaagcagac
     5701 cttatagctt ccaaaacaac gtctttcaaa ataccctttt ctctttcaag ctcgctcaac
     5761 gcatttaaaa tatccttgtt cataacaacg cctccattaa aaatcctcta tcactctatt
     5821 aatttttgcc aaatctttca gctcaatcgt aaaaatatct tctcctgatt tgattacaat
     5881 attattctcg ttcttttccg ttaatatgcc ttttacagtc ttcttgtcat caattggttt
     5941 atataatctt gcttccacta atttatttaa gtttttcaaa tagtcttttt ctgttttgaa
     6001 aggtctttcc actcccggcg aagaaacttc caaaaaatat tgctccttga ttaaatcaac
     6061 ctcatcaagc ttcttattga gtactctgct tatatcagcg gtatcatcca cagtaattcc
     6121 cccgggctta tctatgtaga cttttaaata cttaaagggc ccctctttaa catactcgat
     6181 atcaaataat tcaatatccg tagattttag tatttcttct gtaatttttc tgactgaaga
     6241 tacgatatct ttcttgttca tatttactcc tttaccctgc aatcagaaag agtggggtca
     6301 cccactcttt ctgtcaaaat tcttcttttt aatagtatat cacaaatatt ttataaaatc
     6361 aaacattatt ttataaatag cacaatgaaa aatagtgctt ttttgctcgc tccgctcaaa
     6421 atttcaagta ttgtttgaaa atatctatga aaaaggtgca ccttatgtat ggaaatacat
     6481 acaacgaaaa acacacatta cttgaattat tgagaactca gttataaaga caggacaaat
     6541 caatcacaca ttaaaaatag aaatctggtt cgtttcatcc atatttttca ggcagccgtg
     6601 tttttccaaa acctctatta cagtctttga tacttttgct ctatttataa tatcttcaat
     6661 tgatataaat tcggaaattt ctctctcagc ctttatgttt gccgccgcat ttgccccaag
     6721 cccttgaaga cccatcaatg gtggcagtat accgtcctcg gtcagaagaa attttctgtc
     6781 gtcggattta tataaatcaa cttttgtaaa tttgaaacct cgcttgtaca tttcatatgc
     6841 tacttcaaga acagtaatta aatttttttc ctttgctgtc ttatcatttc ctttttcttc
     6901 aagagattta attgtattca atatatggtt ttgacctttg attacaatat ctgcatcaaa
     6961 gtcatcaacc ttggtggtga aatatgttgc ataaaaagct tccggataat gaactttata
     7021 gtaagcaatt tttactgaca tggttacata agcaaccgca tgtgccttgg gaaacatata
     7081 tttaattgta ttgcaggagt ctatgtacca ctgaggaaca ttctgtgcct tcatcgcctc
     7141 ctcatcttct tttcgaagtc cttttccttt tcgaactctt tccattatgt caaaagccgt
     7201 ctttttttct actcctattt gaattaaata tgtcattata tcgtcacgag tagatataac
     7261 tcccccgatt ttcgcataac ctttttttat tatatcctgc gcattgttaa tccagacatc
     7321 cgtcccgtgt gaaagaccgc tgattctgac caagtcagaa aatgattttg gctttgtatc
     7381 cttaatcatc tgccttacga aagaagtacc gaattcaggt attgccaatg ttcccgtgct
     7441 gcaatttatt tcatccaatg aaattccaag tatttcagga ctgttaaaaa tggccattgt
     7501 gcttgggtca tccataggaa tatctttgct ttccagtcct gttaaatcct ccagcatgcg
     7561 tataatactt ggagtatcat gaccaagtaa atcaagcttt aaaattcgtc ccgaaatgga
     7621 atggtagtca aaatgtgttg ttatcgttcc cgacttttca
//
LOCUS       DAOSOW010000037         7644 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_9584_length_7644_cov_3.981552, whole genome shotgun sequence.
ACCESSION   DAOSOW010000037 DAOSOW010000000
VERSION     DAOSOW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9584_length_7644_cov_3.981552"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /gene="tyrS"
                     /locus_tag="PLD67_01815"
     CDS             complement(<1..1023)
                     /gene="tyrS"
                     /locus_tag="PLD67_01815"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005982668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOT21295.1"
                     /translation="MEKGNVFDVLVERGYVEQCTHEEEIRELLGKESVTFYIGFDPTA
                     DSLHIGHFIQIMVMSIMQKYGHRPIALLGDGTTMIGDPSDRTGMRSVMSFETITKNAE
                     NFKKVFEKFLDFTDGKALMPRNSEWLLPLNYLEFMREVGVHFTISKMIAADCYKNRMD
                     QGLTFFEFGYMLMQAYDFYILNEKYDCKMQLGGNDQWSNIIAGVELTRKKAGKQVYGM
                     TFALLTNSEGKKMGKTEKGALWLDRNKTTPYEFYQYWRNIDDADVEKCLALLTFLPMD
                     EVRRLGALKGAEINKAKEILAFEITKMIHTEEDAVNAQEAARALFGGGADSENIPTTE
                     LTRAELG"
     misc_binding    complement(1060..1256)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1601..2401
                     /locus_tag="PLD67_01820"
     CDS             1601..2401
                     /locus_tag="PLD67_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015374629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOT21296.1"
                     /translation="MKRRIIALTLALVIALSSTSAFAATTNCTSVNYKYNLKDFIKTN
                     TSNYANKNTQYKITVNGKTVDLKSIDWKTVLKQYAPAQKPAAPVEKPSPAPAPVEKPA
                     PKPAPVEKPAPAPAPVEKPAPAPAPAPVENNTGVPSSNLTYEQKVVELVNIERQKAGL
                     PALKMDSAISNVARTKSKDMAVNNYFAHQSPTYGSAGDMLRQFGISWRAWGENIAAGQ
                     RTPEIVVDAWMNSPGHRANILSSNFSKIGVGYATNSSGRPYWTQIFTN"
     gene            complement(2470..3486)
                     /locus_tag="PLD67_01825"
     CDS             complement(2470..3486)
                     /locus_tag="PLD67_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HOT21297.1"
                     /translation="MDYNELLNKAREKMAPRCRVCKECNGVVCKGEVPGVGGKGSGNA
                     FKVCIDFLASVKINMDTIYKNEGQDTSISLFGKNFKYPFFAAPIGGMVLNYNGSMTEA
                     EYAEAIVNGTLKAGCAGFTGDGPMDNLFLDSLPIIKKANGIAVPTIKPWKNDKALEKI
                     KMIEEVNAMAFAMDIDSAGLINLALAGKPVSAKSVEELKELTDATNLPFIIKGVMTAK
                     GAEKAYQSGAYGIVVSSHGGRVLADAPATCAMLPEIREAVGDKLKIFVDGGIRSGADV
                     FKAIALGADAVLIGRPYAIAAFGGGIEGVEMYTEKIGAELRETMIMTGCKNLSDITRD
                     KIIL"
     gene            complement(3711..6362)
                     /locus_tag="PLD67_01830"
     CDS             complement(3711..6362)
                     /locus_tag="PLD67_01830"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOT21298.1"
                     /translation="MAINIPSKYNPKEFENKIYKIWMDENCFRAEINKDKKPFTIVMP
                     PPNITGQLHMGHALDQTLQDVLIRWKRLMGYEALWLPGSDHASIATEVKVVERLKKEE
                     GKSKEDIGREEFLKRAWKWKEEFGGRIVEQVKRLGNSCDWSRERFTMDDGCNEAVTEF
                     FVRLYERGLIYKGKRIINWCPDCLTTLSDIEVEHEDTAGKYYYVKYPFADNKDEYFII
                     ATTRPETIFGDVAIAAHPEDERYKHIIGRKVIVPLVGRIIPIIADEYPDMEKGTGALK
                     ITPAHDPNDFEIGLRHNLEQISCMNEDGTMNEIAGKYKGMNRFECREAFVKDLEENGY
                     LIKTENTDHNVGTCYRCHNIIEPRISDQWFVKMKPLAGPALKVVENKEMQLVPDRFTK
                     IYNHWLENIRDWCISRQLWWGHRIPAYYCQDCDEMMVQKVAPEKCNKCGSTNIIQDED
                     VLDTWFSSGLWPFSTLGWPHETEDLKYFYPTDVLVTGYDIIFFWVIRMVFSALEVTGQ
                     SPFKYTFIHGLVRDAEGRKMSKSLGNGIDPLDIIDNYGADALRFMLMTGISPGNDTRF
                     NYDRLESSRNFANKLWNASRFVLMNIDGDLTDEERARKNYKTEDKWIISRVNKAAKEA
                     TEYLEKFELGMAAQRVYDFIWNEYCDWYIEIVKPRLYGDDGDDKDTARFVLIRVLKDM
                     LKLLHPFMPFITEEIWSYLPDTSSRLVRANWPEYTEEEISEEAEENIEFIMEAVRSIR
                     NTRAEMNVLPSRKARAIFIPSNTKARLFIESGEKYFSTLANITEVKIVNDKKEIGEDT
                     ASSVIDGTEIYLPLADLIDYEKEIERLEKEKTRLEGELKRVNGKLSNERFMSKAPENI
                     VNEEKEKLQKYQLMMEKVLERLEQMKK"
     misc_binding    complement(6465..6671)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6823..>7644
                     /locus_tag="PLD67_01835"
     CDS             6823..>7644
                     /locus_tag="PLD67_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOT21299.1"
                     /translation="MDLREEALKLHKDNKGKIEVISKVRVENAKDLSLAYTPGVAEPC
                     KEIHANPELIYEYTNKGNMVAVVTDGSAVLGLGNIGSRAGMPVMEGKAVLFKSFANVD
                     AFPICIESQDVDIVVQTVKQIAPSFGGINLEDIAAPRCFEIEERLKKELDIPVFHDDQ
                     HGTAVVVLAGIINALKAVGKKIENVTAVVSGAGAAGIAISKLLLSSGINDVIICNSRG
                     ILDKEDERLDPIRKEMALKTNKANKKGALSDALKGADIFIGVSAPNIVTKKMIESM"
BASE COUNT         2264 a         1569 c         1221 g         2590 t
ORIGIN      
        1 accaagttca gctcttgtta attctgtagt cgggatattt tctgaatcag ctccgcctcc
       61 aaataaagct cttgcagctt cctgtgcatt aactgcatct tcttccgtat gaatcatctt
      121 agttatttca aatgcaagaa tctctttagc tttatttatt tcagcgcctt ttaatgctcc
      181 caatcttctt acttcatcca taggtaagaa tgtaagaagt gccaagcatt tctccacatc
      241 ggcatcatca atgtttctcc agtactgata gaattcataa ggtgtggttt tattcctgtc
      301 aagccacaat gctccttttt cggttttccc catttttttg ccttcgctgt ttgtcaataa
      361 ggcaaatgtc atgccataaa cctgtttgcc tgcttttttt cttgtcagtt caacaccggc
      421 tattatattt gaccattggt cgtttccgcc caattgcatc ttgcagtcgt acttttcatt
      481 taaaatatag aagtcgtaag cctgcataag catatagccg aattcaaaga atgtaagacc
      541 ctggtccatt cgatttttat agcaatctgc tgcaatcatt ttgctgattg taaaatgtac
      601 acccacttcc ctcatgaatt caaggtaatt caaaggtaaa agccattcgg aatttcttgg
      661 cataagtgct tttccatccg taaaatcaag aaatttctca aatacctttt taaagttttc
      721 tgcatttttg gttatggttt caaatgacat aaccgagcgc attccggttc tgtcactggg
      781 gtctcctatc attgttgtcc cgtcacctag taatgctata ggtctgtgcc cgtatttctg
      841 cattattgac attaccatta tttgtataaa gtggcctatg tgaagactgt ccgctgtcgg
      901 gtcaaatcct atataaaatg ttacggattc ttttcccaat aattctctta tttcttcttc
      961 atgagtgcat tgctccacat accctctttc aactaataca tcaaaaacat ttcctttttc
     1021 catctattgc cctcctaact aaataagggt tatttcatcc gaaaggacga gataacccgt
     1081 ggtaccacct taatttgcct aagcctcttg ctgatttaac ggacagataa gccgctgtgc
     1141 ttttcacaca gaactccgga atcgtatttc acactttgtg ctgtgtatct ttcaccaccc
     1201 gatacactct ctaaattgta accaaaatgt tacttaagtt ccttcacagt catgataaag
     1261 tattattgga ataataatat aaaatttaag tgttgtcaag aagtaaattt gttaattaaa
     1321 tacaaccctt acaaataatg ccctaataat taacctttta ttaagtattt ttgaataaac
     1381 tatatatatt tgtcataatt atgtatttat ttcttttgtt tgtaaaatag tgtaatatta
     1441 ttgacacatt tggcattata agaagttatt gattgttttt ttaggggaat tgcagtgcta
     1501 taattctgat atggcgaaaa ctcattaaca agttctagct tttcaaagga acaggcctta
     1561 cctgtttcga aaaaaatctt agactatata ggagtaaatt atgaaaagaa gaattatagc
     1621 attaacatta gcgttagtaa tagcgttatc atcaacatca gcatttgctg caacaacaaa
     1681 ttgcacatcg gtaaattata agtataatct taaagatttt atcaaaacta acacaagcaa
     1741 ctatgctaac aaaaacacac agtataaaat tactgttaac ggaaaaacag tagatttaaa
     1801 atcaattgac tggaaaacgg ttttaaaaca atatgcaccg gcacaaaaac cggcagcacc
     1861 tgttgaaaaa ccatctccgg ctccggcacc tgttgaaaaa ccggctccaa aaccggctcc
     1921 ggttgaaaag ccagctccag ctccagcacc ggttgaaaaa ccagctccag ctccggctcc
     1981 ggcacctgtt gaaaataata caggagttcc ttcgtcaaat ttaacatacg aacaaaaagt
     2041 agtagaactt gtaaatattg aaagacaaaa agcaggctta ccggcattaa aaatggattc
     2101 agcaatttca aacgtggcaa gaactaaatc taaagatatg gcagtaaaca attattttgc
     2161 ccaccagtca cctacttatg gcagtgcggg cgatatgtta agacagtttg gaatcagctg
     2221 gcgtgcatgg ggtgaaaaca ttgcagccgg tcaaagaact cctgagatag tggtagatgc
     2281 atggatgaat tcacccggac acagagcaaa catcttatca tctaacttct caaaaattgg
     2341 agttggatat gcaacaaact caagcggaag accatattgg acacaaatat tcactaatta
     2401 aaattaatta caatattaaa aatgtgcaag acttgactaa gttttgcaca tttatttttt
     2461 tattttggtt tataaaataa ttttatctct tgttatatcg cttaagtttt tgcaaccggt
     2521 cattatcatt gtttctctta attcagcacc gattttttcg gtatacattt caacaccttc
     2581 tataccgcca ccaaaagcag caatggcata tggtcttcct attaatactg catctgctcc
     2641 caatgctatt gctttaaata catcagctcc cgagcgaatt cctccgtcaa cgaaaatctt
     2701 aagcttatct cctactgctt ctcttatttc agggagcatg gcacatgtag ccggagcatc
     2761 agctaaaacc cttcctccgt gagaagatac cacaatacca taggcgcctg actgataagc
     2821 cttttccgct cctttagctg tcattactcc tttaatgatg aaaggaagat tggttgcatc
     2881 tgttaattct ttaagctctt caacactctt tgcagaaaca ggcttgcctg ccaatgctaa
     2941 atttataagg cctgcagaat caatgtccat tgcaaatgcc atagcattta cttcttcaat
     3001 catttttatt ttttccaaag ccttgtcatt tttccatggt ttaatagtag gtactgcaat
     3061 tccatttgct ttctttatta ttggcaggct gtccaagaat aaattatcca taggtccatc
     3121 tcctgtaaac ccggcacatc ctgcctttag tgtaccgtta acaattgctt cggcatattc
     3181 tgcttctgtc atggaaccgt tgtaatttaa aaccatgcct ccgatgggag ctgcaaagaa
     3241 tggatattta aaattctttc caaacaatga aatagaagtg tcttgtcctt catttttgta
     3301 aatagtgtcc atattaattt taacagaagc cagaaaatca atacaaacct taaatgcatt
     3361 accgctgcct ttacctccaa caccgggcac ttcacctttg catactaccc cgttgcattc
     3421 cttgcaaacc ctgcatcttg gtgccatttt ttctcttgct ttgtttaata attcgttata
     3481 atccatgttc ttgtctcctt ttatcataca ttattgttat aaataatctt gtcattcttt
     3541 catcttttta gtaattagat tcttgactct cagaattatc aaatgtttac cacaccacga
     3601 tattaatacg ttttgtcatc ctgagcgtaa gcgaaggatc tcatgagatt cttcgggcta
     3661 aagccctcag aatgacaaag atattattaa taacatgaag tatctcatct ttattttttc
     3721 atttgttcta atctttcaag tactttttcc atcatcagtt gatatttttg aagtttttct
     3781 ttttcttcat taacaatatt ttccggagcc ttgctcataa atctttcatt gcttaactta
     3841 ccgttaactc tttttagttc gccttcaagc cttgtttttt ctttttcaag tctttcgatt
     3901 tctttttcat aatctattaa atcagctaag ggcagataaa tttctgttcc gtcaataact
     3961 gaagaagccg tatcttcgcc tatttctttc ttatcattta caatttttac ttccgtaata
     4021 tttgctaagg tcgaaaaata tttttctcct gattcaataa atagtcttgc ttttgtattt
     4081 gaaggaataa atattgccct tgctttcctt gaaggcagta cattcatttc tgcccttgtg
     4141 ttacggatac ttcgaactgc ttccattata aactcaatat tttcttctgc ctcttcagaa
     4201 atctcttctt cagtatattc aggccagttt gctctcacca atcgtgaaga tgtatccggc
     4261 aggtatgacc atatttcttc tgtgataaat ggcataaagg gatgcaaaag ctttaacata
     4321 tcctttaaaa ccctaatcaa tacaaacctt gccgtatcct tgtcatcacc atcatcgcca
     4381 taaagtctcg gtttaacaat ttctatatac cagtcgcagt attcattcca tataaaatca
     4441 taaaccctct gtgcagccat tcccagttca aatttttcaa gatattcggt agcttcctta
     4501 gctgctttgt taactcttga aattatccat ttatcttctg ttttatagtt tttccttgcc
     4561 ctttcttcat ctgtcaaatc tccgtctata ttcattaata caaatcttga agcattccac
     4621 agtttatttg caaaattacg gcttgattcc aatctgtcat aattaaatcg tgtgtcgttt
     4681 ccgggagaaa tccctgtcat aagcatgaaa cgaagagcat ctgcaccgta gttgtcgata
     4741 atatcaagag ggtctatgcc gtttcctaat gatttgctca tttttctgcc ttccgcatct
     4801 ctcaccaaac catgaataaa tgtatactta aagggtgatt gacctgtaac ttccaaagct
     4861 gaaaatacca ttcttataac ccagaagaat ataatatcgt atcctgtaac caaaacatcc
     4921 gtaggataga agtattttaa atcttctgtt tcatggggcc agcctaaggt tgaaaacggc
     4981 caaagtccgg atgaaaacca tgtatcgaga acatcttcat cctgtataat attggtgctg
     5041 ccgcatttat tgcatttttc gggagcaact ttttgaacca tcatttcatc acagtcctgg
     5101 cagtaatatg caggaatcct gtggccccac cacaattgtc ttgaaataca ccagtctctt
     5161 atattttcaa gccagtgatt gtaaattttc gtaaagcggt ccggaaccag ctgcatttct
     5221 ttattttcaa ctacctttaa tgcaggtcct gccaatggtt tcatttttac aaaccactgg
     5281 tcggaaattc gaggctcaat aatattgtgg cacctgtaac atgttcctac attgtggtcg
     5341 gtattctctg tcttaatcaa atacccattt tcttctaggt cctttacaaa tgcttctctg
     5401 cattcaaaac gattcatgcc tttgtattta ccggcaattt cattcatggt tccgtcttca
     5461 ttcatacagc ttatttgctc taggttatga cgaagtccaa tttcaaagtc gttagggtca
     5521 tgagccggag ttattttcaa tgctcctgta cccttttcca tatcagggta ttcatccgct
     5581 ataattggaa ttattcttcc aactagtgga acaattactt tccttccgat tatatgttta
     5641 tatctttcat cttcaggatg tgcagctatg gcaacatctc cgaaaattgt ttccggtctg
     5701 gttgtagcaa tgataaaata ttcatcctta ttatcagcaa atggatattt tacgtaataa
     5761 tacttgcctg cagtgtcctc atgctcaact tctatatcgg aaagagttgt taggcagtca
     5821 ggacaccaat taataattct ttttccttta tatataagtc ctctttcgta cagccttaca
     5881 aaaaactcag taacggcttc attacagcca tcatccatgg tgaatctttc tcttgaccaa
     5941 tcgcaggaat tacctaatct tttaacctgc tcaacaatcc tgccgccaaa ttcttctttc
     6001 catttccaag ctcttttaag aaattcttcc ctgcctatat cttccttgga ttttccttct
     6061 tcctttttaa gtctttcaac tacttttact tcggttgcaa tgcttgcatg gtcagagccg
     6121 ggaagccaca aagcttcata acccatcaat ctcttccacc tgatgagaac atcctgcaga
     6181 gtttggtcca aagcatgccc catatgaagc tgccctgtaa tattcggagg cggcatcaca
     6241 atcgtaaatg gttttttatc tttgtttatc tctgctctaa agcaattttc atccatccag
     6301 attttataaa ttttattttc aaattccttc ggattgtatt tgcttggtat gttaattgcc
     6361 ataataccct tcctttcttt ttattatcat tgtcggaata acaagtagta tcattccggg
     6421 gtagagcctg tatgggttat aaataaaaaa ccaccggatt tcatcaaaag gacgagaaac
     6481 cccgcggtac cacctaattt ccattacggc tcttaaaaaa tataacggtt actccgttta
     6541 atcttacttt tacttcagat taaatgctca aaagctacct tcggcacaca ttgacctatg
     6601 aagactttca tccggtggtc ttcactctct gacaagttta tgcacttact cctctttttc
     6661 atcgcaaata tttaattaca tatcatatta ttaaaattta atgtttttgt caacaacttt
     6721 ttcttaattt tgttttttaa atgaatataa aattcaaata atgtataaga cgtaaagtta
     6781 tggtataata attaataatt aaggacgaag gagaaagtaa atatggattt aagagaagaa
     6841 gctttaaaac tacacaaaga taacaaaggt aaaattgaag ttataagtaa ggtcagagta
     6901 gaaaatgcaa aggatttaag cttagcctat acacccggag tagctgagcc gtgcaaggaa
     6961 atccatgcga atcctgaact aatatatgaa tatactaata agggtaatat ggttgcggtt
     7021 gttactgacg gctctgcagt attaggtttg ggtaatatag gttcaagagc cggaatgcct
     7081 gttatggaag gtaaagcagt attatttaaa tcatttgcca atgtagatgc ttttcctatt
     7141 tgcatagaaa gtcaagatgt tgatatagta gttcaaactg ttaaacaaat tgcgccgtcc
     7201 tttggcggta taaatctgga agatattgct gcaccaaggt gctttgaaat agaagaaaga
     7261 ctaaagaaag aattggatat tcccgtattt catgatgatc aacatggtac tgccgtcgtt
     7321 gtattagcag gaataatcaa tgcgttaaaa gcagtaggta aaaaaatcga aaatgtaaca
     7381 gctgttgtaa gtggtgccgg tgcagccggt atagctatat ctaaattgct tctgagttca
     7441 ggaatcaatg atgtaattat atgtaacagc agaggtatat tagataaaga agatgaaagg
     7501 cttgacccga ttagaaaaga gatggcctta aagacaaata aggcaaacaa aaaaggagct
     7561 ctttcagatg ctttgaaagg agcggatata tttataggtg tttctgcacc taatatagtt
     7621 acaaaaaaaa tgattgaaag catg
//
LOCUS       DAOSOW010000038         7563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_9721_length_7563_cov_2.976825, whole genome shotgun sequence.
ACCESSION   DAOSOW010000038 DAOSOW010000000
VERSION     DAOSOW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9721_length_7563_cov_2.976825"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..694)
                     /locus_tag="PLD67_01840"
     CDS             complement(146..694)
                     /locus_tag="PLD67_01840"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOT21300.1"
                     /translation="MKIYTKTGDKGQTSLYDGTRVDKDSLRVESYGTVDELNSYIGFA
                     MKFVNDESINESLKKIQMRLFFVAGELATVEEGKYKYKVKEEDIVALEKIIDDYLPKI
                     SGVDKFIVPGSSEASAALHVARTICRRAERRIISLKRHEEVSEILLKFVNRLSDVLYT
                     YARFLESNLTIMDFEKAYNEKL"
     gene            complement(703..1572)
                     /gene="rsmA"
                     /locus_tag="PLD67_01845"
     CDS             complement(703..1572)
                     /gene="rsmA"
                     /locus_tag="PLD67_01845"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOT21301.1"
                     /translation="MDEIKLYSPKVIKDILDRYGFKFSKSLGQNFLIDGNIIKRIVEI
                     AGLDEKSGVLEIGPGFGTLTQLLCKKANKVIAIEVDKSLTEVHKGTLDYPNLKIIYDD
                     FLKVDVNKLIEEEFKGLDVKIVANLPYYITTPIIMKILEEKYKVSKIVVMVQKEMAQR
                     LNSKPGTKDYGAITLAVQYRADTNIAMIVPNSVFMPKPKVDSAVIEFRILPKPRIDVC
                     DEKMLFKVIKASFAQRRKTILNGLSNSLNFSKDIINESLVSAGMNPGIRGEKLTLEEF
                     GRISDEISKRVKD"
     gene            complement(1565..2113)
                     /gene="rnmV"
                     /locus_tag="PLD67_01850"
     CDS             complement(1565..2113)
                     /gene="rnmV"
                     /locus_tag="PLD67_01850"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HOT21302.1"
                     /translation="MIKEIIVVEGKADVSAVKRAVDAQVISTNGLGINDKIINVIKKA
                     SKNKGIIILTDPDYPGKKIRNILASQIENCKHAYIPRDKANKDGDIGVENASKEAIIE
                     ALKNARAEVAENRQEFSYQDMVRYGLVGNDNASKRRSAIGDELGIGYCSAKQFLKRLN
                     SFGISREELEDAIKKTIEDING"
     gene            complement(2106..2765)
                     /gene="udk"
                     /locus_tag="PLD67_01855"
     CDS             complement(2106..2765)
                     /gene="udk"
                     /locus_tag="PLD67_01855"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014970864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOT21303.1"
                     /translation="MKKPIFIGVSGGTGSGKTTIVNRICSEVPEKSVAIMEHDSYYKD
                     QSSMTYEERCQTNYDHPFAFDTDLFVEHIKELKKGNSIQKPIYDYSIHNRKKETIIVE
                     PKEIIIVEGLLVFYEERIRELLDIKIYVDTDADIRILRRIIRDMNERSRSLDSVITQY
                     LNTVRPAHEQFIEPSKKYADIIVTEGGNNLVAVDLMVTKIKSLLNMSNSDTITHGANF
                     D"
     gene            complement(3103..3744)
                     /locus_tag="PLD67_01860"
     CDS             complement(3103..3744)
                     /locus_tag="PLD67_01860"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HOT21304.1"
                     /translation="MKIKIIYPILILTLAMSFIITYGISEGKFETYAFAEIFEKSEEA
                     EESEAYDEIKDIDEITARNMEAKLTLKELKYHIERDSLEQETSLEYKNDQYKKETTSR
                     GGFRFNNAMDMVATAYDLSYESCGKYPDDPEYGITASGTKAQPGTVAVDPDVIPLGTK
                     LYIASTDGSPDYGFATALDTGSAIKGYRIDLFMENNQDAMDFGRRQVKVYILD"
     gene            complement(4091..4960)
                     /locus_tag="PLD67_01865"
     CDS             complement(4091..4960)
                     /locus_tag="PLD67_01865"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF005491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HOT21305.1"
                     /translation="MKEIPKIMAVQDMSGVGRCSLTVIIPIMSALGCQVCPLPTALLS
                     NHSEYKEFYFFDFTDHMEEYYSYWEKNDFWFDCVYSGFIGSEKQINIIVDIIEKVKNK
                     NNKALIVIDPVMGDCGCTYATYNSEMITKMSQLVKKADIITPNLTEACILLGRKYYSD
                     KITLPQTKECLKDLCSLGPKISLITGIITDEEEHINVCYDSEQDKYWLAPFSYIDKRY
                     PGTGDLFTSLFLGYYMKGKSLPEAMEEAARFVSLSVRISSEAEAPSSEGVIFEKIMKE
                     LYNEISEYKYTSI"
     gene            complement(5387..6877)
                     /locus_tag="PLD67_01870"
     CDS             complement(5387..6877)
                     /locus_tag="PLD67_01870"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOT21306.1"
                     /translation="MAFIINEPSRTFGEYLLLPNLTTKDCIVENVDLSTPLTRFKRGK
                     APEIKLNIPFSSAIMQSVSDNNMAVALAKNGGISFIYCSQPIESEAEMVRKVKKYKAG
                     FVISDSNLTPEHTLADVLNLKAITGHSTMAITEDGSPTGKLLGIVTGRDYRTSRDSLD
                     KKVKDFMTSREDLIVGREGITLSEANDIIWEHKLNSLPIVDKDFNLVYMVFRKDYSED
                     KENPNSLLDAKKRYVVGAGINSRDYKERVPALVEAGADILCIDSSEGFSEWQHETINW
                     IKQRYDGKVKVGAGNVVERDGFKYLADAGADFVKVGIGGGSICITREQKGIGRGQASA
                     VMDVAAARDEYFEKTGEYVPICSDGGIVLDYHITLALAMGADFVMLGRYFAGFDESPT
                     NKIKYGSGYVKEYWGEGSNRARNWARYDLGGNAGLHFEEGVDSYVPYAGKLDDGLKTT
                     LHKIKSTMCNVGTKTIKELQQNARLTVVSATSLTEGGAHDVILKNQ"
     gene            complement(7094..>7563)
                     /locus_tag="PLD67_01875"
     CDS             complement(7094..>7563)
                     /locus_tag="PLD67_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOT21307.1"
                     /translation="ADEMSKKLNRSITPIKSLEEYCKKSDIIITATVSKEPIVEADWI
                     MPGTTCINVGGYEYTFDAVRKAQKVVVDTWEDVKHRMASTIAIMANEGLFADEEIYAE
                     IGEIVIGDKIGRENDQEIIYYNSVGMGIEDVAIATRVYRTAQEKGIGTKLIYW"
BASE COUNT         2183 a         1596 c         1042 g         2742 t
ORIGIN      
        1 gagcgaagcg aaggatctca tgagattctt cgagctaaag ctctcagaat gacattccat
       61 gggctactaa tatcaatgac accctgcaat atatgaatac gctttgtcat cctgagcgaa
      121 gcgaaggatc tcataatagt gtattctaaa gtttttcatt ataagccttt tcaaaatcca
      181 ttatggtcaa attactttct aaaaatcttg catatgtgta taatacatcc gacagcctgt
      241 ttacaaactt caaaagtatt tcacttactt cttcgtggcg ttttaaagat ataattctcc
      301 tttcagccct gcggcaaatc gttcttgcaa catgaagtgc agctgaagct tccgaagagc
      361 cgggtactat aaatttatcg actccgctta ttttaggaag ataatcatct attatttttt
      421 ccaatgcgac tatatcttct tctttaacct tatacttgta tttcccttct tcaacggtag
      481 ccaattctcc cgccacaaaa aataagcgca tttgaatttt cttaaggctc tcgtttatac
      541 tttcatcatt aacaaatttc atagcaaatc cgatataaga atttaattcg tcaaccgttc
      601 cgtatgattc cactcttaat gaatccttat ctactcttgt gccgtcatat aaagatgttt
      661 gacctttatc accggtcttg gtataaattt tcataattcc tcctaatctt ttactctttt
      721 acttatttca tccgatattc ttccgaattc ctccagtgtt agtttttctc ctcttatacc
      781 cggattcatg cctgcactaa ccagagattc atttattatg tctttagaaa aatttaaact
      841 attgcttaat ccgttaagta ttgtctttcg tctttgagca aatgaagctt taatcacttt
      901 aaaaagcatc ttttcatcac atacatcgat tctgggtttc ggtaaaattc taaactcaat
      961 aacagcagaa tcaacttttg gttttggcat aaataccgaa tttggaacta tcattgcaat
     1021 atttgtgtct gccctgtact gaacggcaag agtaatagca ccataatcct tggttccggg
     1081 ttttgaatta agcctttgag ccatttcctt ttgtaccatt acaacaattt ttgaaacttt
     1141 atatttttcc tccaatattt tcataatgat gggtgtagta atgtaataag gtaaatttgc
     1201 aactattttt acatcaagcc ccttaaattc ttcttcaatt aatttattta catcaacctt
     1261 caaaaagtcg tcataaataa tttttaagtt ggggtaatca agtgtaccct tatgaacttc
     1321 tgttaagctt ttatctacct ctatggcaat taccttgttt gctttcttgc acaaaagctg
     1381 ggtcaaggtg ccaaagcccg gtcctatttc caatactccc gacttttcat ccaaaccggc
     1441 tatttctaca attcttttta ttatattccc gtcaataagg aaattctgtc ccagactctt
     1501 gctgaattta aatccgtacc tgtcaagtat gtccttaata acttttggtg aatacagctt
     1561 tatttcatcc attaatatcc tcgattgttt ttttgattgc atcttctaac tcttccctgc
     1621 ttataccgaa ggaattaagc ctctttaaaa attgttttgc gctgcaatat cctataccta
     1681 attcatcacc tatggcactt cttcttttag atgcattatc attcccaacc aatccatagc
     1741 gcaccatgtc ttggtaagaa aactcctgtc tgttttctgc gacttcagcc cttgcatttt
     1801 tcagcgcttc aattattgct tcttttgaag cattttcaac tcctatgtcc ccgtctttat
     1861 ttgccttatc cctgggtatg tacgcatgct tgcagttctc tatttgggaa gcaagaatat
     1921 ttctaatttt ctttcccgga tagtccgggt cggtcagtat aataattccc ttgtttttcg
     1981 atgccttttt aataacatta ataattttat catttatacc taatccattg gttgaaatca
     2041 cctgtgcatc aacagccctc ttgactgccg aaacatctgc cttgccttca accacaataa
     2101 tttccttaat caaaatttgc tccatgtgta atagtatccg aattgctcat atttaacaaa
     2161 gactttattt ttgtaaccat caaatcaact gccactaaat tattgcctcc ttcggttaca
     2221 ataatatcag catatttttt gcttggctct ataaactgtt catgagcggg tcttaccgta
     2281 tttaaatact gggtaataac ggaatcaaga cttctgctcc tttcattcat atctcttata
     2341 attcttctaa gaattcttat atctgcatct gtatcgacat aaattttaat atcgagaagt
     2401 tctcttattc tttcttcata aaataccaaa agtccctcca caattattat ttcctttggc
     2461 tcaacaataa ttgtttcctt tttcctgtta tgtatgctgt aatcataaat aggtttttga
     2521 attgaatttc cttttttcaa ttccttaata tgctcaacaa acaaatccgt atcaaatgca
     2581 aatggatggt cgtaatttgt ttgacatctt tcttcataag tcatcgaact ttggtcttta
     2641 taataagaat catgctccat aattgctacg cttttttcgg gaacttctga gcaaattcga
     2701 tttacaattg ttgttttacc ggaaccggtt ccacccgata cccctataaa aattggtttc
     2761 ttcataattt cctctcaaat gtttattata ataataatat acaacatttt ttcctatttt
     2821 accatataca aataaatcat aacaaaaaaa taatgttcaa tcattaaata atatgctaag
     2881 tgtcattctg agagctttag ctcgaagaat ctcatcttta taaaacctga gatccttcgc
     2941 atacgctcag gatgacaatc aaagagcata tttgtttatc gtggcttggt aaaaacatga
     3001 tatttctgaa tctcttttta tacatgaaaa aattgctgtc tccagcaatt tttccttcgg
     3061 ggacatttct cgcatgtccc cataactttt aacttaatga tattagtcta atatatagac
     3121 ttttacctgt ctcctgccaa aatccattgc atcttggttg ttttccataa acaagtctat
     3181 tctgtaccct tttatcgctg agcctgtatc aagggctgtt gcgaaaccat agtcaggact
     3241 gccatcagta gaagcgatat acagcttcgt acctaaaggt attacatcag ggtctaccgc
     3301 aaccgtaccc ggctgagctt ttgtgccgga tgctgttatt ccgtattcag ggtcgtccgg
     3361 atatttccca cagctttcat atgataaatc ataagctgta gctaccatat ccattgcatt
     3421 attaaatctg aaaccaccac gagaggttgt ttctttttta tattgatcgt ttttatattc
     3481 taaagatgtc tcttgttcta aactgtctct ctcaatatga tactttaatt cttttaatgt
     3541 aagttttgct tccatattcc ttgcagttat ttcatcaatg tctttaattt catcatatgc
     3601 ttctgattct tcagcctctt ccgacttttc aaaaatctct gcaaaagcat aggtttcaaa
     3661 cttaccttca ctgataccat aagttataat aaagctcatt gccaatgtta aaatcaaaat
     3721 agggtatatt attttaattt tcatattacc accaacctta tatttgaatt gactaagacg
     3781 tctattaagg tataaatttc ttccggaaat ttatgttttg cataagtatg agcttatgca
     3841 ttattggcag tcccttttta aaaaactcat aatttttggc ataaggagaa ttatacttta
     3901 aaaaaaaagc ccggtcaagt ctttttttga aaaatctgcc ttctaatgcc tatattgttg
     3961 caattgccta ctttttatcc aatcctttat tttaactttt ctgctataat aaggcagaat
     4021 ttttagaaca ttttccaaaa atggcataca aaaacagcct gtaaaaggct gtttttcact
     4081 gcaatatatt tcatatactc gtatatttat actccgaaat ttcattgtac aattctttca
     4141 taatcttttc aaaaattact ccttctgagc ttggtgcttc agcctcggaa cttatacgaa
     4201 ccgaaagcga aacaaatctt gctgcttctt ccattgcttc aggaaggctc ttccctttca
     4261 tataataacc caagaataag gaggtaaata aatcccccgt acccggatat cttttatcta
     4321 tatagctgaa aggtgccagc caatacttgt cctgctcgct gtcgtaacaa acattgatat
     4381 gttcctcctc atctgttata attccggtta ttaatgatat tttaggtccc agactgcata
     4441 aatcctttag gcattccttt gtttgaggaa gagtaatttt gtcgctgtaa tattttcttc
     4501 caagcaatat acaagcttct gtcaaattcg gtgttataat atcagctttt tttaccaatt
     4561 gtgacatttt tgtaatcatt tcactgttat atgtggcata agtgcaaccg cagtcaccca
     4621 ttacagggtc tatcacaatc aatgctttat tatttttatt cttaaccttt tcaatgatat
     4681 cgacaataat gtttatttgt ttttcagagc ctataaaccc gctgtaaaca caatcaaacc
     4741 aaaaatcatt cttttcccag tatgaataat actcttccat atggtctgtg aaatcaaaaa
     4801 aataaaattc cttgtattcg gagtgattgc ttaataaagc tgttggtaag gggcacacct
     4861 gacatcccaa agcagacata atcgggataa tcacagtcaa tgagcatctc cctactcccg
     4921 acatatcttg aacagccata attttaggaa tttctttcat aatttatcct ttcattatag
     4981 ggattttacc tgatacgggg acattccttc agtccccata catattttaa cttattcttg
     5041 tactttcaaa aggtacagat ttatcaaatt ttaagaggac agttttcata tcatacgcat
     5101 tgaatgcatc aaatttacta aaaaaaatga tttctatata aatagaaatc attttttaca
     5161 gggtaaagat ttattaaata actctttaca aggggggcta atttatttca taatatatat
     5221 tcgagcttgt acaatgtttt tttggtgtta ttctcaaaaa atattttacc agttttttac
     5281 accatattct acaaaattct ctacatatag tgtagcaaat ccattgcaca tttgcaagaa
     5341 aaattatttg aaaattagaa atttctgtaa agttttacta atctgcctat tgattcttca
     5401 aaataacatc atgagctccg ccttcggtaa gacttgttgc agaaaccaca gtaagccttg
     5461 cgttttgctg aagttccttt attgtttttg taccaacgtt gcacatggtc gattttattt
     5521 tgtgaagtgt tgttttaaga ccgtcatcaa gcttgcctgc ataaggaaca tatgaatcaa
     5581 cgccttcttc aaaatgaagt cccgcatttc cgcctaaatc atatcttgcc cagtttcttg
     5641 ccctgttgga gccttctccc caatattcct ttacgtatcc gctcccgtat tttattttat
     5701 ttgtagggct ttcatcaaat cctgcaaaat accttccaag catcacgaaa tcagctccca
     5761 ttgccaaagc aagggttata tgataatcca ggacaattcc tccgtcagag catattggaa
     5821 catattctcc ggttttttca aaatattcat cacgggcagc agcaacatcc attacagccg
     5881 aagcctggcc tcttcctatt cccttctgct cacgcgttat gcatatcgag cctccaccta
     5941 tacctacctt aacaaagtca gcacctgcat cggccaaata tttaaaacca tccctctcaa
     6001 ctacattgcc ggcacctacc ttaaccttgc cgtcatatct ttgctttatc caattaattg
     6061 tttcatgctg ccattcacta aaaccttcgg atgagtctat acaaagaata tccgctcctg
     6121 cttcaacaag ggcaggaacc ctttccttgt aatctcttga attgatacct gctcccacaa
     6181 catatctttt cttagcatcc aacaaggaat tagggttttc cttgtcttcg gaataatcct
     6241 ttctaaatac catgtatacc aaattaaaat ctttgtctac gatgggaaga gaattcaact
     6301 tgtgctccca aattatatca ttggcttccg aaagagtaat accttctctt cctacgatta
     6361 gatcttctct gcttgtcata aagtccttaa cctttttatc cagggaatcg cgagatgtcc
     6421 tgtaatccct gccggttaca attccaagaa gctttcctgt aggagagccg tcttctgtta
     6481 tggccattgt cgaatgaccg gttatagctt tcaaatttaa aacatccgct aaggtatgct
     6541 ccggtgttaa gtttgaatcg cttattacga atcctgcttt atatttttta actttcctta
     6601 ccatttctgc ttcactttct atcggctgtg agcaatatat aaaagatata cctccgtttt
     6661 tagccaaagc tactgccata ttgttatcgg aaacagattg catgatagct gaagaaaaag
     6721 gtatattaag ctttatttca ggagctttgc ctcttttaaa ccgagttaac ggtgtactta
     6781 aatcaacatt ctcaacaata cagtcttttg tggtcaaatt aggtaaaagt aaatattctc
     6841 caaacgttct tgaaggctcg tttataataa atgccatctt aatctccttt tcttattgtt
     6901 aatcgttttt taataattgc tatcatacca tattactaat gtcaactcaa gataaacaag
     6961 aaaatattca taaattttat accatattta taacaaacta aaagaattga attccgcatg
     7021 atacggaatt caattctaat agtatagaat atttaattat tgaagggact tattaaaaat
     7081 aattaatata tttttaccag taaatcagtt ttgtacctat tcctttttcc tgagctgttc
     7141 tgtaaactct tgttgctatt gcaacgtctt caattcccat tccgacagaa ttataatata
     7201 tgatttcctg atcattctct ctgccaattt tatcgccaat tacaatttca ccaatctcag
     7261 catatatctc ttcatctgca aataaacctt catttgccat tattgcaatt gtgcttgcca
     7321 ttctgtgctt tacatcctcc caggtatcga caacaacctt ctgcgctttt ctcacggcat
     7381 caaatgtata ttcatagccg cccacattta tacaagttgt tcccggcata atccaatctg
     7441 cttctacaat aggctcttta gaaactgttg ctgtaataat gatatctgat ttcttgcagt
     7501 attcttccaa agattttatc ggtgtaatgc tgcgattaag tttcttgctc atttcatcag
     7561 caa
//
LOCUS       DAOSOW010000039         7446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_9944_length_7446_cov_3.038696, whole genome shotgun sequence.
ACCESSION   DAOSOW010000039 DAOSOW010000000
VERSION     DAOSOW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7446
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9944_length_7446_cov_3.038696"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLD67_01880"
     CDS             complement(<1..224)
                     /locus_tag="PLD67_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21308.1"
                     /translation="MVNNKQDKKTPVKLYTYIFFLALTAILLMIYLFNNYTISDYVLL
                     IVFSILTAIAETFWILLPKIGAVSVSFALT"
     gene            complement(447..1070)
                     /locus_tag="PLD67_01885"
     CDS             complement(447..1070)
                     /locus_tag="PLD67_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21309.1"
                     /translation="MSFEEMEHFLNNIKSVLSSKVIIDSNNEIREIHVLADNSRHSKQ
                     IARDIRTALLSKFNEDVDYKIISVAQIDKNMSFNSDFRLLYEGYINETTPEKIKIKTK
                     LTWDGKEFIGEAEGFKSDKQSLIVAARSTLDAIRQAIEFDCFILEDIQYTKIAGEKAL
                     IVAINQINNGKENISVGASIIEDNQIDSAIKATLNAINRKICLLIKD"
     gene            complement(1234..1956)
                     /locus_tag="PLD67_01890"
     CDS             complement(1234..1956)
                     /locus_tag="PLD67_01890"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOT21310.1"
                     /translation="MENILKKINLVFAAAIIIIMLVSIFMNLTRTAETIDAAGENKIK
                     KIKILIDPGHGGVDQGASGDMKIGEAPINLAISEKLMSFLEGSGFEVEMTRYDDSGLY
                     TELSGTIRDKKNEDLKNRVELINNSNADLVISIHLNSFPQKQYYGAHVFYQKSNEATT
                     KLAADIIQESMKSILDKSNQRVPQIKKGIKIMDDTKVPVILIECGFLSNNKEERKLIS
                     EDYQEKTAWAIYTGLLKYLNEL"
     gene            complement(2069..3514)
                     /locus_tag="PLD67_01895"
     CDS             complement(2069..3514)
                     /locus_tag="PLD67_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002580016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOT21311.1"
                     /translation="MKIKIVLKVLGIVLFWEAALMVPSLLISIIDNSYEIKAFIIAIS
                     ATALAGFLLSRFKFVKPEMRKREGYAAVALSWLVMSLFGALPFYLSGSIPSYIDALFE
                     TVSGFTTTGSSIVRNVEIMPRGLLFWRSFTHWIGGMGVLVFTMALVPSIGGHPVFLMR
                     AESPGPSPGKLVPKIGESSKILYIIYTVMTAILVISLILAGMPVFNSFIYAFGTAGTG
                     GFSMDNLSVGAYNNPWIEWIITIGMFLFGVNFALYYLLIRKEFKHIFKNEEFKLYCFY
                     VVLGIALVVINVMPKFDYNFFEALRHSAFTVSTLVTTTGYSAVDFNLWPMFSKSVLLT
                     LMIFGACAGSTGGGVKSVRILVLIKTLYYEINHTIHPKSVQRIKIDGKALDDDTLKSI
                     LIFFFAYIFIIILSVIIVSLDNFDFMTSFSAVIATVSNIGPGFEVVGPMGNYADFSYL
                     SKLIFIADMILGRLEIMPVIALFSPVIWRRN"
     gene            complement(3544..4899)
                     /gene="trkA"
                     /locus_tag="PLD67_01900"
     CDS             complement(3544..4899)
                     /gene="trkA"
                     /locus_tag="PLD67_01900"
                     /inference="COORDINATES: protein motif:HMM:NF007033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOT21312.1"
                     /translation="MKIIIVGGGKVGYTLAQQLSTEDHDITLVDNDADVLTHADETLD
                     IMCIKGNGASAEILKSAGIYEADILLAVTDSDELNMICCVIGKKLGAKHTVARVRNTD
                     YSDEYKMLREELELDMVINPEMEAAAEIAQMIQYPFAADVETFTNNLIELVEFRVLET
                     DPIINKNLLELNNHLPSKVLFCSVMREEEVFIPKGNFVFLPDDLVQVIGERSSISSFF
                     KYLGRNSNKAKNVTIIGGSRTAIYLTWLLEEMGLNVKIIELDKERCLILNDVLTQALI
                     ICGDGTDQEVLDSENIEAADVIVALTDRDEENLISSLYAHQCGVPKVILKINRQNYFP
                     IVQKLGLDSIISPKFITATNILRYIRALNNSQGIAVEKLYRILDNKAEVAEFTVKYSP
                     DLTDIKLQDLNLKEDVLIAAILRNKKIIIPSGSDVIKEHDKVIVVTKTGFITDLNEIL
                     V"
     gene            5303..5608
                     /locus_tag="PLD67_01905"
     CDS             5303..5608
                     /locus_tag="PLD67_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOT21313.1"
                     /translation="MRIEEHPILKFNKVKKIRFIFNGQEMEGYEGETIAAALHAAGVK
                     VLGKSLFLHRPRGFYCAIGNCSSCLMTVNGVANVKTCITDLEEGMIVETQEGKGVIV"
     gene            5605..6696
                     /locus_tag="PLD67_01910"
     CDS             5605..6696
                     /locus_tag="PLD67_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT21314.1"
                     /translation="MKQAEVVIIGGGPAGLCAALSAAKSGSKVLIIDKNKKLGGQLIK
                     QTHMFFGSEKQYASKRGIDIPEILIDELFKYKENVEISTEATVVGMYEDGIITVLEKE
                     DNYYKIEPKAVVVATGAYEKFLSFPNNDLPGIYGAGAVQTLMNVYGIKPGTRVLMVGA
                     GNIGLIVSYQLMQAGVEVKAILDAAPKIGGYLVHASKIRRMGVPILTSHTVKEAIGNE
                     FLEKAVICKVDEKFNPIEGTEITYDVDVMCISVGLTPLNELLAMRGCEMKYIPQLSGL
                     VPVRNENYETTVENVFVAGDATGVEEASAAMVEGFLAGLCAAAKIGYKPDDFMERKND
                     YEEQLNNLRSGPAGKHILAGIEQIRRSYV"
     gene            6689..7177
                     /locus_tag="PLD67_01915"
     CDS             6689..7177
                     /locus_tag="PLD67_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HOT21315.1"
                     /translation="MFEKTGIPSTEMIIKKFPPIDRINKGAVAVIECYKEIPCNPCET
                     ACKFDAIKVGEDINNIPEARFENCTGCAICLSKCPGLAIMIVDGSKSESTVEVKIPYE
                     FLPLPKEGQIVKGLNREGNYITDVKVLKILNPKSFDRTPVITIEVDRKFLYEIRNIRV
                     EG"
     gene            7179..>7446
                     /locus_tag="PLD67_01920"
     CDS             7179..>7446
                     /locus_tag="PLD67_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOT21316.1"
                     /translation="MDENTIICRCSDVTLKEIRELIKDGYVTFDEIKRITRAGMGPCQ
                     GKTCGQLIMREIANITGQNMKDIKFQTNRPPVVGVKLGLIAEEAR"
BASE COUNT         2426 a         1269 c         1312 g         2439 t
ORIGIN      
        1 aaggtgagtg caaagctaac tgatacagca cctatttttg gtaatagtat ccaaaatgtt
       61 tcagctatag ctgttaatat tgaaaaaaca ataagcaaaa catagtcgct tattgtgtag
      121 ttgttaaata aataaatcat tagcaatatt gcagtaagcg caagaaaaaa tatatatgta
      181 taaagtttaa cgggagtttt cttatcttgc ttattgttaa ccatttttca tcctttatat
      241 gaagggctga cctcctaatt tactcttaag cccagctaaa tccagcgccg gcagctaata
      301 ccattgctac tattgttgct aatataggaa ataacttttt catctgaaac ctccacagat
      361 attctctgct cagttcgcta gtagaacagg aaggtttcgc tacgctaatt ctttaaataa
      421 ttatttgatg ttaagggtca gccctatcaa tcttttatca ataaacagat ttttctgtta
      481 attgcattta atgttgcttt tattgcagaa tcaatttggt tatcttcaat aattgatgca
      541 ccgactgaaa tattttcttt gccgttatta atctgattaa ttgcaacaat cagagctttt
      601 tctccggcta ttttggtata ttgaatgtct tctaaaatga aacaatcaaa ttcaatagct
      661 tgtctgattg catccaaggt tgatcttgca gctactatca gggattgctt atctgattta
      721 aagccctcgg cttctcctat aaattctttg ccatcccagg tgagtttagt ttttatttta
      781 attttttccg gagttgtttc atttatataa ccttcgtata gaagtctaaa atccgagttg
      841 aaggacatat ttttgtctat ttgtgctacg cttattattt tataatcaac atcttcattg
      901 aacttcgaaa gcagtgcagt gcgtatatct cttgcaattt gcttggaatg cctgctattg
      961 tcagccagta catgtatttc gcggatttca ttgtttgaat cgattataac tttagatgaa
     1021 agaacagatt taatattatt taaaaaatgc tccatttctt cgaaactcat atttatcctc
     1081 caagtattgt taattaatta taatatattg ctattagaat tacaacattt atttagaaaa
     1141 tctctaaaat tttcacattt ttttcaataa tattacaaaa aaaatacact attccatccc
     1201 gcaacggaaa gtgtatttaa ttttattgat taattataat tcatttaaat atttaagtaa
     1261 acctgtatat atagcccaag cagttttttc ttgataatct tctgatatta gttttctttc
     1321 ttccttgttg ttcgataaaa atccgcattc gattaaaatc accggaactt tagtatcatc
     1381 cattattttt atgccctttt taatttgagg cacccgttga ttactcttgt caagaatact
     1441 tttcatgctt tcctgtatta tatcagctgc caattttgta gttgcctcat tgcttttttg
     1501 ataaaaaaca tgagccccgt agtattgttt ctgcggaaaa gaatttaaat gtattgatat
     1561 tactaaatct gcatttgagt tattaatcag ttcaactctg ttttttaaat cttcattttt
     1621 tttatctctg attgtgccgg aaagttctgt gtatagaccg ctgtcatcat atcttgtcat
     1681 ttcaacttca aatccgcttc cttccaaaaa actcataagt ttttcggata tggctaaatt
     1741 aatgggggct tctccgattt tcatatcacc gcttgctcct tggtctactc cgccatgccc
     1801 cgggtctata agtattttta tttttttaat cttattttca cctgcagcat caattgtttc
     1861 tgcagtcctg gttaagttca taaaaatact taccagcatt atgattatta ttgctgcagc
     1921 aaataccaaa ttaatctttt ttaaaatatt ttccataata ctacctctca taatttgtct
     1981 atatttaaat ttatgagaag agaatattta tatgacaaaa aaggaagaag ggtaccttgc
     2041 agtgggtgaa gggtataggg taatttattt agtttcttct ccaaattaca ggtgagaata
     2101 aagcgataac cggcattatt tccagccttc caagtatcat atcagcaata aaaatgagtt
     2161 tgctcagata agaaaagtct gcataatttc ccattgggcc tacaacttca aaaccgggtc
     2221 ctatattgct gacagttgct attactgccg aaaatgatgt cataaaatca aaattatcca
     2281 gagaaacgat aataacggat agtattatta taaaaatata tgcaaaaaag aatattaaga
     2341 tactttttaa agtatcatca tctaaggcct tgccgtcaat tttaattctt tgtacagact
     2401 ttggatgtat tgtatgattg atttcataat ataaagtttt aataagtact aatattctta
     2461 ctgatttaac cccgcctccc gttgagcctg cgcaggcacc aaaaatcatt aaggttaaaa
     2521 gcactgactt tgaaaacata ggccacaaat taaaatccac cgctgagtag cctgtagttg
     2581 taaccaaggt agaaaccgtg aaagcagaat gtcttaatgc ttcaaaaaag ttgtaatcaa
     2641 atttaggcat tacattaatt acaacgagtg ctattcccag gactacgtaa aagcagtaga
     2701 gcttgaattc ttcattctta aaaatatgtt taaattcttt tctgattaga aggtaatata
     2761 gagcgaagtt aactccgaat aaaaacatac ctattgtaat aatccactca atccaaggat
     2821 tgttgtatgc tcccacactc aagttatcca tggaaaagcc tcccgttccc gccgttccaa
     2881 aagcataaat aaatgaattg aatacaggca taccggcaag aatcaatgat ataacaagaa
     2941 tggcagtcat aactgtataa attatatata gaattttaga cgattctcct attttaggaa
     3001 ccaattttcc gggtgagggg ccggggcttt cggcacgcat taaaaatacc ggatgccctc
     3061 ctatagacgg gactagagcc attgtaaata ccaaaacacc cataccgcct atccagtgag
     3121 taaagcttct ccaaaataat aaccctctgg gcataatttc aacattacgc acaatgcttg
     3181 agcctgttgt tgtaaaccct gaaactgttt cgaacagggc atcaatataa gacggtatgg
     3241 agccgcttaa ataaaaggga agagcaccaa agagggacat tacaagccag cttagtgcaa
     3301 ctgctgcata accttctctt tttctcattt ccggtttaac gaatttaaat cttgacaaga
     3361 gaaaacctgc taaggcagta gctgaaattg ctataatgaa agccttgatt tcatatgagt
     3421 tgtcaattat tgatatcagt aaagaaggaa ccattaatgc agcttcccaa aataaaacta
     3481 ttccaagtac cttaagtact atcttaattt tcacatttgc ctccaattca tgagactata
     3541 tcgctatacc aatatttcgt ttaggtcggt tataaatccg gtctttgtaa ccactataac
     3601 cttgtcgtgc tctttaatta catcacttcc gctaggaatg ataatttttt tattgcgtaa
     3661 aatcgcagct attaaaacat cttctttcag gtttaaatcc tgcaatttaa tatcagtcaa
     3721 atcaggagag tatttgactg taaattcagc aacttcagcc ttattgtcaa gtatcctgta
     3781 aagtttttca actgcgatac cctgtgaatt attcagtgct ctaatatacc ttaatatatt
     3841 ggtggcggta ataaacttag ggcttattat gctgtcaagt cctaattttt gtactattgg
     3901 aaaataattc tgtctgttta tttttaaaat tactttaggc acaccgcatt gatgcgcata
     3961 aagagagctt attaaatttt cttcgtccct gtcagttaat gcaacaataa catcagctgc
     4021 ttcgatattt tcactatcca aaacttcctg gtcagtacca tctccacaga ttatcaatgc
     4081 ctgtgttagt acatcattta gtattaggca tctttccttg tcaagctcaa ttatttttac
     4141 attcaaaccc atttcttcaa gaagccaggt taaataaatt gctgttctgc taccgcctat
     4201 aattgtaaca tttttagctt tatttgagtt tctgcctaaa tatttgaaaa aacttgagat
     4261 gctgcttctt tcccctataa cctgaactaa atcgtccggt aaaaatacaa aattcccctt
     4321 tggtataaag acctcttctt ctctcattac tgaacaaaac aatacctttg aaggcaaatg
     4381 attgtttagc tctaataggt ttttatttat tatggggtcc gtttccaaaa ctctgaattc
     4441 aaccaattca atcaagttat tggtaaatgt ttcaacatct gcagcaaaag ggtactgaat
     4501 catttgagct atttcagctg ctgcttccat ttcaggatta attaccatat ctaattcaag
     4561 ttcttctctt aacattttat actcatcgga ataatcagtg tttctaaccc ttgcaacagt
     4621 atgctttgct cctaattttt ttccaataac gcagcaaatc atatttaatt catcactgtc
     4681 tgttactgcc aataaaatgt cagcttcata tattcctgca ctttttaata tctctgcact
     4741 tgctccgtta cctttgatac acataatatc aagtgtttcg tctgcatgtg tcaacacgtc
     4801 ggcatcattg tctactaatg ttatatcgtg atcttcggtt gataattgtt gagccaatgt
     4861 atagccaact tttcctccgc cgactattat aattttcatt ttttcctcca ctactaagtt
     4921 ttgatttatt attaacagat ttattatatg tctaatttaa aaaatgtcaa atattttaat
     4981 tatttttacc ctggggagaa aatttttacc ttagtatttt tcttggataa gtataagcat
     5041 tcttgtatct gatatctttt ttgctgtgtt ttatatagca gtatcagcaa tattatatcc
     5101 ataatgccaa aagctaatca taatgtcggg aattagacaa agtgtcggaa tgttgacgca
     5161 caaagagatt ttgtgataaa ataaaatact gcaatttaaa agtttattga atatattcaa
     5221 tttcagatat tggcatgact attgcttact atattggagg agtgtcccca taaccaatat
     5281 acatacttta caggaggtat ctatgagaat tgaagaacat cccattctaa aatttaataa
     5341 agtaaaaaaa ataaggttta tttttaacgg gcaggaaatg gaaggctatg aaggagaaac
     5401 aattgctgct gctctgcatg ctgcgggagt gaaggttttg gggaaaagtt tgtttttgca
     5461 cagaccaaga ggcttttatt gcgcaattgg aaattgctct tcatgtttaa tgaccgtaaa
     5521 tggagtggca aatgtaaaaa cttgtattac tgatttggag gaaggaatga tagtagaaac
     5581 ccaggaaggc aagggagtga tagtatgaaa caagcggaag ttgttattat aggcggagga
     5641 ccggcaggtc tttgtgctgc cctaagtgct gcaaaaagtg gctccaaggt attgattata
     5701 gacaaaaata aaaaattagg cggacagctt ataaagcaaa ctcatatgtt tttcggctcc
     5761 gaaaaacaat atgcctccaa aaggggaatc gatatacctg aaatattgat tgatgaacta
     5821 tttaaatata aggaaaatgt cgaaatctca actgaagcaa cagtagttgg aatgtatgaa
     5881 gacggaatta taactgtttt ggaaaaggaa gataattatt ataaaataga gccaaaagct
     5941 gttgttgttg caacaggagc ttatgaaaaa tttttaagtt ttcctaacaa tgatttaccg
     6001 ggaatttacg gagcaggagc tgttcagact ctgatgaatg tttacggcat aaagccggga
     6061 acaagagtat tgatggtagg cgcaggaaat atagggctta tagtaagcta tcagcttatg
     6121 caggcaggag ttgaggtaaa agcaatactt gatgcagcac caaagatagg tggttatttg
     6181 gtacatgctt ccaaaataag aaggatgggt gttccaatat tgacttccca caccgtaaaa
     6241 gaagcaattg gcaatgagtt tttagaaaag gctgtaatat gtaaggttga cgaaaagttt
     6301 aatcccatag agggaacaga aattacttat gatgttgatg taatgtgtat ctccgtaggt
     6361 ttgactccgc taaacgaact tcttgcaatg agaggttgcg agatgaagta tatccctcag
     6421 ttaagcggtc tggttccggt aagaaatgaa aattatgaaa ctactgttga aaatgtattt
     6481 gttgcaggag atgctaccgg agtggaagaa gcaagtgctg caatggttga aggatttctg
     6541 gcaggtttgt gcgctgcagc aaaaatagga tataagcctg atgatttcat ggaaagaaaa
     6601 aatgattatg aagaacaatt aaataattta cgctcaggtc cggcaggaaa gcatatatta
     6661 gcaggcattg agcagataag gaggtcatat gtttgaaaaa acaggaattc caagcacaga
     6721 aatgattatt aaaaagtttc ctccaataga caggattaac aaaggtgcag ttgcagtaat
     6781 agagtgctac aaggaaatac cctgcaatcc ttgtgaaact gcatgtaaat tcgatgcaat
     6841 aaaagtaggg gaagatataa acaatattcc cgaagctaga tttgaaaact gcacgggctg
     6901 cgcaatatgc ttaagcaaat gtccgggact ggcaattatg atagtggacg gctcgaagtc
     6961 agagagtacg gttgaagtaa aaatacccta tgaatttttg cctctgccga aagaaggaca
     7021 aattgtaaaa ggacttaaca gggaaggaaa ttatattacg gatgttaagg ttttaaaaat
     7081 attaaacccg aaatcttttg acagaacacc ggtaattaca atagaagttg acagaaagtt
     7141 tttatacgaa ataagaaata tcagggtgga ggggtaagat ggacgaaaat actataatct
     7201 gcagatgctc ggatgttaca ttaaaggaaa tcagagagct tatcaaagat ggttatgtta
     7261 cgtttgatga aataaaaaga attaccagag cgggcatggg accctgccag ggcaagacat
     7321 gcggtcagtt gataatgagg gaaattgcaa atattacagg tcaaaatatg aaagatataa
     7381 aattccaaac caacaggcct cctgttgtgg gagttaaatt aggactaata gctgaggagg
     7441 caagaa
//
LOCUS       DAOSOW010000040         7395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_10059_length_7395_cov_3.888965, whole genome shotgun sequence.
ACCESSION   DAOSOW010000040 DAOSOW010000000
VERSION     DAOSOW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10059_length_7395_cov_3.888965"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="PLD67_01925"
     CDS             <1..1410
                     /locus_tag="PLD67_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOT21317.1"
                     /translation="TGRSIPGVQAGDIIGTVQETIVVEHRIMVPYGISGEVVEIKEGS
                     FTVEETVCVVKKDNGEDVNLTMMQKWPVRKGRPYKEKLVPEAPLLTGQRVIDTLFPIS
                     KGGVAAVPGPFGSGKTVVQHQLAKWADAEIVVYIGCGERGNEMTDVLMEFPELKDPRT
                     GETLMKRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRWAEALR
                     EMSGRLEEMPGEEGYPAYLASRLAQFYERAGRVISMGTDGREGALSAIGAVSPPGGDI
                     SEPVSQATLRIVKVFWALDAQLAYSRHFPAINWLRSYSLYVDRLGPWFDENVAKNWTE
                     MRNEVMKLLQEEAELNEIVQLVGVDSLSVYDRLKLEATRSIREDYLHQNAFHEIDTYS
                     SLEKQHKLLSLILRYYHKGIEALDNGADFKALSELEVRERIGRFKYTEEKDIKEVYED
                     IEKQIDKEVLALSASKEVE"
     gene            1412..2788
                     /locus_tag="PLD67_01930"
     CDS             1412..2788
                     /locus_tag="PLD67_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOT21318.1"
                     /translation="MIKEYRTISEVAGPLMLVKDVENVAYDELGVIELPSGETRSCRV
                     LEINGSNALVQLFESAAGINLAESKVKFLGRGMEFAVAEDMLGRVFDGLGRPIDNGPE
                     IIPEKRLDINGLPMNPSARDYPNEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AAQIARQSRVLGTESKFAVVFAAIGITYEEADFFISDFRRTGAIDRTVLFMNLADDPA
                     IERISTPRVALTAAEYLAFEKDMHVLVILTDITNYAEALREVSAARKEVPGRRGYPGY
                     LYTDLATIYERAGRKRDHKGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRDLY
                     RKNIKPPIDVLPSLSRLKDKGIGRGKTREDHSDVLNQLFAAYARGKEAKELMAILGEA
                     ALSDADKFFAKFADEFERRYVSQGYETNRTIEETLEIGWNLLTILPKGELKRIRDEYL
                     EKYYPEKE"
     gene            2804..3499
                     /locus_tag="PLD67_01935"
     CDS             2804..3499
                     /locus_tag="PLD67_01935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOT21319.1"
                     /translation="MARLNVNPTRMVLTSLKNRLRVAVKGHKMLKDKRDELMKRFLEL
                     ARENKILREQVEALIGNVYSNFVIASAVMSSEVLEEALMYPKQGVTIEVGRKNVMSVD
                     VPVFNFKTTTDDTANIYPYGFANTSAELDNAIENLSNIFPELLKLAAVEKEVQLLAAE
                     IEKTRRRVNALEYIMIPQLEETIKYIQMKLDENERGNQTRLMKVKDMMVKEAIEEKQQ
                     FTKNAIDEYTNNK"
     gene            3669..4703
                     /locus_tag="PLD67_01940"
     CDS             3669..4703
                     /locus_tag="PLD67_01940"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008423626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOT21320.1"
                     /translation="MILNELKIGNLTARIPIVQGGMGVGVSLANLAAAVANEGGIGTI
                     SGVQIGFREPDFFTDPIGANLRAIKKEIKKAREKTKGIIAMNFMAAMNNYELYVKKAV
                     EEKIDLIVSGAGLPMALPRLVKGSNTKIVPIVSSEKAAKIIIRSYLKSDRLPDAVVVE
                     GPLAGGHLGFKMEELLENKCMALIDIVKEVKLLLSTFEEKYKVKIPLIAAGGIRTKED
                     IIELKEAGADGFQIASLFVPTVECDAHQNFKSAYINARDDKINIIKSPVGMPGRAIET
                     NFLTQTERIIKKCHKCMPNCNPNEIPYCISEGLINSVEGRDGLIFSGAKLNNVNKMTT
                     VKEVINNLIG"
     gene            5324..6877
                     /locus_tag="PLD67_01945"
     CDS             5324..6877
                     /locus_tag="PLD67_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009139327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HOT21321.1"
                     /translation="MSGDTLQIFVSMIIYMAVVIGIGLYFAKRANESSENYFLGGRTL
                     SPLVTAMSAEASDMSGWLLMGLPGVAYWYGLSDAAWTAIGLAAGTYLNWLFVAKRLHN
                     YSIAAGNSITLPDFLSNRFKEKNKIIMSISAVFILVFFIVYASSCFVTVGKLFNALFG
                     FKYQYMMILGAAFVLFYTFIGGFLAESASDTLQAFVMIIALVTIFFVGTLAAGGVAQV
                     IENAKEIPGFFEFFGIASPNVTDGVQEVVNGVPQYNEAGEYGLYTILSTLAWGLGYFG
                     MPQVLLRFMAIRDSNELKTSRRIATVWVVISLGAATAIGIIGRAVFPTTLLTYSDSES
                     IFIVLSSNLLPAFLAGFVMAGILAATISSSDSYLLIAASAFSKNIYQHLIKKDATDKQ
                     VMNVSRIILVLICIIGIFIALDENSVIFTIVSFAWAGFGATFGPIIIFSLFWKRTTRE
                     GAITGMLAGGIMVFVWKLVLRPMGGLFGVYELLPAFIVSCLFIYAVSKMTAAPSEEIQ
                     KEFEAVMKM"
     gene            complement(6924..>7395)
                     /locus_tag="PLD67_01950"
     CDS             complement(6924..>7395)
                     /locus_tag="PLD67_01950"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOT21322.1"
                     /translation="SGKTTVCKMLSKKLNMEFFDMDDNIVSMENKSINEMFEISEEYF
                     RNAETRCAKFLSNKTSLIIAAGGGIIKRKENIDYLKQNSIIVFLNRPPEKIIEDIDIK
                     TRPLLREGRDKVFTLYNERLHLYKKYCDIEVLNDKTLDDAVNEIIKRVIPYISS"
BASE COUNT         2426 a         1155 c         1633 g         2181 t
ORIGIN      
        1 acgggaagaa gtatacccgg tgtacaggca ggagatataa taggtactgt tcaggaaaca
       61 atagttgtag agcatagaat tatggttcct tacggaattt caggggaagt tgttgaaata
      121 aaggaaggaa gctttactgt tgaagaaaca gtatgtgtag taaaaaaaga taacggtgaa
      181 gatgttaacc taaccatgat gcaaaaatgg cctgtaagaa aaggacgtcc atataaggaa
      241 aaattagttc ctgaagcacc tttgctaaca ggtcagaggg ttatagatac tttattccca
      301 atttcaaagg gtggtgttgc agctgttccg ggacctttcg gaagcggaaa gacggtagtg
      361 cagcaccagc tggcaaaatg ggcagatgct gaaatagttg tatatatagg ctgtggagag
      421 cgtgggaatg aaatgaccga cgttctaatg gagttccctg agttaaagga cccaagaaca
      481 ggggaaacat tgatgaaacg taccgttctt atcgcaaata catccgacat gcctgttgca
      541 gcacgtgaag catcaattta tacaggaatt acaatagccg agtatttcag agacatgggt
      601 tattccgtag cactgatggc agactctaca tctcgttggg cagaagcact tcgtgaaatg
      661 tccggtcgtc ttgaagaaat gcccggtgaa gaaggttatc ccgcatactt ggcatcacgt
      721 cttgcacagt tctatgaaag agcaggaaga gtaataagta tgggtacaga tggaagagaa
      781 ggagcattgt ctgcaatcgg agcagtatca cctccgggcg gagatatttc agaaccggta
      841 tctcaggcta cccttcgtat agtaaaagtt ttctgggcac tcgatgcaca acttgcatac
      901 agcagacact tcccggcaat caactggtta agaagctatt cattgtatgt tgacagatta
      961 ggcccatggt ttgatgaaaa tgtagcaaaa aattggactg aaatgcgtaa tgaagttatg
     1021 aagcttcttc aggaagaagc agaattaaat gaaatagtac agcttgtcgg tgttgactca
     1081 ctgtcagtct atgacagatt aaagttagag gcaacacgct ccataagaga agactatctg
     1141 catcaaaatg cattccatga aattgatact tattcatccc ttgaaaaaca gcataaactt
     1201 ctgtcactga tacttcgata ttatcacaag ggaatagaag cattagataa tggagcagat
     1261 ttcaaagcat tatcagaact tgaagttaga gaaagaatag gaagatttaa atatacggaa
     1321 gaaaaggaca taaaggaagt ttacgaagac atcgaaaaac aaatagataa agaagtattg
     1381 gctttatcag caagcaagga ggttgagtag tatgattaaa gaatatagga ctatatctga
     1441 agtagcagga cctttgatgc ttgttaagga tgttgaaaat gttgcatacg atgagttggg
     1501 agtaattgag cttcccagcg gtgaaaccag aagctgcagg gttcttgaaa tcaatggaag
     1561 caatgcattg gttcaattgt ttgaaagcgc cgccggaatc aacttagctg agtccaaggt
     1621 aaagttcctt ggaagaggaa tggaatttgc agttgctgaa gatatgctgg gacgtgtatt
     1681 tgacggactt ggaagaccta tagacaacgg tcccgaaata ataccggaaa agagattgga
     1741 tattaacggc cttccgatga atccttcagc aagagactat cctaatgagt ttattcaaac
     1801 aggtgtttct gccatagacg gtttgaacac attggtaagg ggacagaagc tgcctatttt
     1861 ctcaggctcc ggacttcctc atgctaactt ggcagcacaa attgcccgtc agtcaagagt
     1921 tttgggtact gaatctaaat ttgctgttgt atttgctgca atcggtataa cttatgaaga
     1981 agcagatttc tttatttctg acttcagacg tacgggagca attgacagaa cagtattgtt
     2041 catgaacttg gcagatgacc cggcaattga gcgtatatcc acacctcgtg tggctttgac
     2101 tgcagctgag tatctagcct ttgaaaaaga catgcacgta ttggtaatat tgacggatat
     2161 aacaaactat gcggaagctc ttcgtgaagt atctgctgct cgtaaggaag ttcccggcag
     2221 acgtggttat ccggggtatt tgtataccga cttagctaca atttatgaaa gagcaggaag
     2281 aaaaagagac cataaaggct ccataacaat gataccgatt ctcactatgc ctgaggatga
     2341 caaaacacat ccgattccgg accttacagg ttatattact gaaggacaga ttattctttc
     2401 acgtgatctg tacagaaaga atattaagcc tcctatagat gttttgcctt cactgtcacg
     2461 tttgaaagac aagggtatag gcagaggaaa aaccagagag gatcactctg acgtattgaa
     2521 ccagctgttc gcagcatacg caagaggtaa ggaagcaaaa gaactcatgg ccatacttgg
     2581 agaagcagcc ctatccgatg cagataaatt ctttgcaaaa tttgcggatg aatttgaaag
     2641 gagatatgtg tctcaaggtt atgaaacaaa cagaacaatt gaagaaacac tggaaatagg
     2701 ctggaattta ctgactatac ttcctaaggg agaattaaaa cgtataaggg atgagtatct
     2761 tgagaaatat tatcctgaaa aagaatagca taggggtggt tatgtggcaa gattaaatgt
     2821 taatcctacc agaatggttt taacctcttt aaaaaatcgt ttaagggtgg ctgtaaaggg
     2881 tcataaaatg cttaaggaca aacgtgatga gctcatgaaa agatttttgg agcttgcacg
     2941 agaaaataaa attttgcgtg agcaggttga agcactcatt ggcaatgttt attcgaattt
     3001 tgttattgcc agtgctgtaa tgtcctcgga ggttttggaa gaagccctta tgtatcctaa
     3061 acagggagta accatagagg taggcagaaa gaatgtaatg agtgtagatg ttccggtgtt
     3121 taattttaaa actacaactg atgacactgc aaatatttac ccttacggtt ttgccaatac
     3181 ttcagctgaa cttgacaatg caatcgaaaa tttgtcaaat attttccctg agctgttaaa
     3241 attggcagct gttgaaaaag aagttcaact tcttgcagct gaaatcgaaa aaaccagaag
     3301 aagagttaat gcacttgaat atataatgat tcctcagctt gaagaaacta tcaagtacat
     3361 tcaaatgaag cttgacgaaa atgaaagagg aaatcaaacc aggctcatga aagttaaaga
     3421 catgatggtt aaagaagcaa tcgaagaaaa acaacagttt accaagaatg ctattgacga
     3481 atatacaaat aataaataaa agttaattaa ttattgtcat tctgagtgca ctgtacgaag
     3541 aatctcaaag caggggacac gcctttaaaa ttgaaggaat gtccctgttt tttttacttg
     3601 acaaaagaaa catatgcata tatactttga atatcaaatt aattgatttc taaaataatg
     3661 gtggaaatat gatattaaat gaattgaaaa taggaaactt aactgctaga attcccattg
     3721 ttcagggagg catgggcgtt ggtgtatctt tggcaaattt agcggcagca gtcgcaaatg
     3781 aaggtggtat aggcactata tccggtgttc agataggctt cagagagcct gattttttta
     3841 ctgaccccat aggggcaaat ttaagggcaa tcaaaaaaga aataaaaaag gcaagagaaa
     3901 agacaaaggg tattattgcc atgaatttta tggcagcaat gaacaattat gaattgtatg
     3961 ttaaaaaggc tgttgaagaa aaaatagatt taattgtatc aggcgcagga cttccgatgg
     4021 cacttcctag attagtgaag gggagcaata cgaagatagt tccaatagta tcttctgaaa
     4081 aagctgcaaa aataataatc agaagttatc tcaaatcaga tagactgccg gatgcggtag
     4141 tagttgaagg gcccttggca ggtggacatc tgggttttaa gatggaagaa ttattggaaa
     4201 ataaatgcat ggcattaata gatattgtta aagaggtgaa acttctatta agtacatttg
     4261 aagagaaata taaagtaaaa atccctttaa ttgcagccgg tggaataagg acaaaggaag
     4321 atatcattga gcttaaagaa gcaggagcag atggttttca aatagcaagc ttatttgttc
     4381 ccacggttga atgtgatgct caccaaaatt ttaagtcagc atatatcaat gcaagagatg
     4441 ataagattaa tattatcaaa agtccggtcg gaatgccggg aagagctata gaaacaaatt
     4501 ttctcactca aacggaaaga attataaaaa aatgccacaa atgtatgcct aactgcaatc
     4561 ctaatgaaat tccatattgc atatcggaag ggttgataaa ttccgtagaa ggaagagacg
     4621 gtctcatttt ttcaggtgca aaattaaaca atgtaaataa aatgactaca gtaaaagaag
     4681 ttattaataa tttaattggg taataagatt aagataatat tttaattcct ctaatgcaac
     4741 aaggggacag ttgctataat gataacaact tgtctccttg tcgcattatg acaaggggac
     4801 gggggtgttt tcatgtatga caagggaaag agaattttgt aacatagcag gggatggaag
     4861 catattatga attaaaatac atatttgttt ttattgtggg gacaagacaa tatgtaaaaa
     4921 caattgaatt aaatttatat gaatttttag gtaaaataat tcaaaaaata tcttgacaaa
     4981 gtatttgtat tggtgtagta tgttttttaa tattaatatt tgctgtgaag aggaatagta
     5041 atgaaacatg gacttagaga gaaggttggc cggtgaaata cctttgtccc aatgttgatt
     5101 gaacccgcct cagagcgttt gggagtaaca acccaaaggg aacacccctt atttaaatga
     5161 gatgctgtat ataatgcagt tagagtggta ccgcggacat ccgcctcttt ttgaggcgga
     5221 ttttttaata ataaccctgt atttctaggg tacggggaca caaattctac tgtagggaga
     5281 aagaaaatca taataacaaa atattaaaga aggaggtttc attatgagtg gagatacact
     5341 acagattttt gtatcaatga ttatttacat ggctgttgtt ataggcatcg gattatattt
     5401 tgcaaaacgt gccaatgaga gcagtgaaaa ttatttttta ggaggtagaa cacttagccc
     5461 attggttaca gctatgagtg ccgaagcatc agatatgagc ggttggcttt taatgggact
     5521 tcccggagtt gcatactggt atggcttaag tgatgctgca tggactgcaa taggattagc
     5581 ggcaggaacg tatttaaact ggttgtttgt tgcaaaaagg cttcacaact attcgattgc
     5641 agcaggtaat tcaataacac ttcctgattt tttaagcaat cgtttcaaag aaaagaataa
     5701 aataattatg tctatttctg cggtgtttat tttagtattc ttcattgttt atgcttccag
     5761 ctgttttgta acggtgggta aactttttaa tgcactgttt ggatttaaat atcagtatat
     5821 gatgatatta ggggctgcct ttgtattatt ttacacattt ataggaggtt ttctggcaga
     5881 aagcgcctcc gatacattgc aggcatttgt tatgattatt gctttagtca caatcttttt
     5941 tgtgggaact cttgcagcgg gaggagtagc acaagttata gaaaatgcaa aagaaatccc
     6001 cgggtttttt gaatttttcg gtatagcctc acctaatgta acggatggtg tgcaagaggt
     6061 tgttaatgga gtacctcaat ataacgaagc aggagagtat ggcctttaca cgattttatc
     6121 aactcttgca tggggattgg gatacttcgg aatgccacag gtattattga gattcatggc
     6181 tatcagagat tctaatgagt taaaaacatc aagaagaatt gcaacggtat gggtagttat
     6241 ttccttaggt gctgccacag ccataggaat aataggaaga gcagtatttc ctactacact
     6301 tttgacatac agtgattcag aaagtatttt cattgtactt tcatcaaatt tacttcctgc
     6361 atttctagcc ggttttgtaa tggcagggat tttagcggca acaataagtt catctgattc
     6421 atatttattg attgcagctt cggcattttc caaaaatata taccagcatt taattaaaaa
     6481 ggatgcaacc gacaaacagg ttatgaatgt ttcaaggatt atcttggttt tgatttgtat
     6541 tataggtata tttatagccc ttgatgaaaa cagtgttatt ttcacaatag tatcctttgc
     6601 ttgggcaggg ttcggggcta ccttcggacc gattatcata ttctcattgt tctggaagag
     6661 aacaaccagg gaaggtgcta ttacaggaat gctggcaggg ggtataatgg tatttgtttg
     6721 gaagcttgtt ttaaggccta tgggcgggct ctttggagta tatgaacttc ttcccgcatt
     6781 tatagtatcc tgtctgttta tttatgcagt atctaaaatg actgcggcgc cttcggaaga
     6841 aatacagaaa gaatttgaag cggtaatgaa aatgtaataa ttttggtgcc aaataatttt
     6901 ggtgccaggc acctttggca ccattaggat gatatataag ggataaccct ttttataatt
     6961 tcatttacgg catcatccag tgttttatca tttaaaactt caatgtcgca atatttctta
     7021 tacagatgaa gtctttcatt gtatagggta aatactttat ctcgcccttc tcttaggaga
     7081 gggcgggttt ttatgtcgat atcttctatg attttttccg gaggtctgtt taagaatact
     7141 ataattgaat tttgtttcaa atagtctata ttttctttcc tttttattat tcctccgccg
     7201 gcagctatta taagcgatgt tttattgctt aaaaattttg cgcatcgggt ttctgcattc
     7261 ctgaaatatt cttcgcttat ttcaaacatt tcattaatac ttttattttc catggatact
     7321 atattgtcat ccatatcaaa aaattccata tttaattttt ttgaaagcat cttgcaaacc
     7381 gtagtttttc cgctt
//
LOCUS       DAOSOW010000041         7224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_10366_length_7224_cov_4.512763, whole genome shotgun sequence.
ACCESSION   DAOSOW010000041 DAOSOW010000000
VERSION     DAOSOW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7224
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10366_length_7224_cov_4.512763"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(39..1103)
                     /locus_tag="PLD67_01955"
     CDS             complement(39..1103)
                     /locus_tag="PLD67_01955"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HOT21323.1"
                     /translation="MAHNKIISLILIIFIAVCLTACGTLDMMVGLKDENNPDIADLSD
                     LTDFEIVEDTIGEEALEEPYEEVIETVTISTEEAKMTNIMAYYEDEYGFVVPVNTKIP
                     WEEGIAKATLRSMVRGSETEKRIAQSGLRGVLPEGTEIRGMSIKDGVCRVDFSKNILN
                     TSTLKQEENLISAITYALTEFPTIDKVELLVDGQALSSLTNGYAINKGFERENINLYG
                     SADGVNYTVYYKAPDTEVMGHYVPITFSAAKVGNPVEFVMEKLFEGPPADTTLSNNIP
                     VGINLRDVKVIGDSAVVNLGVEAVNLSEEDYKDMNSIVVLCLEQYGISDVEFNIEGLS
                     FEQAGLYFEDSNVTPVFNQY"
     gene            complement(1174..1974)
                     /gene="pgeF"
                     /locus_tag="PLD67_01960"
     CDS             complement(1174..1974)
                     /gene="pgeF"
                     /locus_tag="PLD67_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOT21324.1"
                     /translation="MYKLNKIKDFEYLTFPIFDGYDELFHCFTTRKGGVSKGDFESMN
                     LGFNTGDDEEDVRENYYILSECLNINLEDIVKTFQSHTDNIRYVTEEDKGRVMKKAKY
                     TDIDGLYTDRKNIALMSFHADCNAVFFYDPVKKIIGLSHAGWKGTMTNITGKMIKALK
                     DLGGNPSDLKAAISPSLGQCCFEVDKDVADMFINTDIKYKDFMIKKGEKYHFDLWGIN
                     KYNMLREGMKEENIEVAGMCTKCRNDLFFSHRGQKGKRGLMSGIIMMK"
     gene            2355..3719
                     /locus_tag="PLD67_01965"
     CDS             2355..3719
                     /locus_tag="PLD67_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOT21325.1"
                     /translation="MIISPVFDKESSKPLYIQLYEYIKTEITSGLLKPDTKLPSIREA
                     SSTLKLSKTTVENAYSQLVAEGYIDNYPKKGYFVCDLSEYNFDIEATKNTVEYYDEII
                     YLNDGVDKESFDISTWKRLYNKVISDSKTNIYNSCSTQGEEFLREEIANFVNTMRGGH
                     TDSRQVIVGAGVQYLLGMLIGIIKEDYNTVAIEKPGFKKAEFVFEDYMFNIRHIEITD
                     DGFPINELRKSDAEVIYISPSHQYPLGTIMPVNKRMELLDWAYKKNALIIEDDYDSLI
                     RYGGAPIPSLQGLDKNDCVVYLGSFSKMLLPSLRISFMVIPKKLLPKYNEIKDRYTQS
                     TSKIDQLVLANFMKDGYMVKHLRKIKRIYKKKNSYLTSNFRNLFGDRAEVISSDSGLH
                     IVCEANTKKDAQQIIKDAQNSRILINIIRIKKNKVMFSLNYSGVDLNKIHEFTILLGK
                     VLFE"
     gene            complement(3757..6492)
                     /locus_tag="PLD67_01970"
     CDS             complement(3757..6492)
                     /locus_tag="PLD67_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004820973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOT21326.1"
                     /translation="MEWYNKGKNEVLKELDTNLSVGLSEKEVKRRIEKYGKNEFEEQA
                     KKSILNKFAAQFADFLIIILLIAAGVSAFVGEREDAVIILAIVVLNAALGIYQEGKAE
                     KSVEALQRLSAPNAKVIREGNLTVVPAADIVPGDVVVLEAGDIIPADIRLFESSNLKV
                     EEASLTGESVPVEKDAKAKIVGTVGIGDRHNMGFSSTIVTYGRGKGVVTSTGHNTEIG
                     NIAVKIQTYEEEETPLQIKLNQLGKVLGTLTIVICIIVFIVGMLQGRQPLNMLLTSIS
                     LAVAAIPEGLPAIVTIVLAIGMNRMAEKNAIVKKLLAVETLGATSVICSDKTGTLTQN
                     EMTVVKAYVDDRILYVEGIGYEPKGDVKLNGRSIGISSLPNLRNLISVGVLANDAKLD
                     NSSGFYKVMGDPTEGAIVAFAGKLGQTSEQLNMIYPRIAELPFDSGRKMMTTFHSNYI
                     EGKIVSFTKGAPDIVINKCSKIALNGRIVDFNKELKDKVRSVNTKFARSALRVLSAAY
                     KVWDRLPEHLTFEQVENDMIFVGLVGMIDPPRPEVKDSIKHCRQAGIETVMITGDYKE
                     TAYAIAKELGMANNEGQAIMGEELDKYSDEELREVVKKTKVYARVSPEHKVKIVTALK
                     ENGHITSMTGDGVNDALALKKADIGVAMGITGTDVAKNTAEVILTDDNFATIVNAVEE
                     GRIIFSNIKKFVFFLLSCNIGEILLVFISILLGWNVPLLPIQLLWLNLVTDSLPAMAL
                     GVENAEPGIMKQPPRSTKEGILDGEMLGGIVFQAIAISFASLFAYYWAMRMYGTGSGL
                     IHVRSVVFTTLILSELLRAFSSRSQNYTLFKIGLFTNIRMIQAVFVSFMLTFVVLYIP
                     QLNEIFDVVPLTLRDWQIVTAFSFIPLLVGELYKDLFKNEMPLSNSERKRRISTL"
     gene            complement(6704..>7224)
                     /locus_tag="PLD67_01975"
     CDS             complement(6704..>7224)
                     /locus_tag="PLD67_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004061932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT21327.1"
                     /translation="SYKDRKTNNTNLAILSSHNFRNPFNEPIKYGAKVAELLNMLGDG
                     KILVQRYGDILDGKRTWDHELSRSNVRPTLPDAVAGDITSAMPYRAMTNILNFIEALN
                     VVVPGFAGTETLIYAPEVKFYSNKVEISTEFETNIKNLYCLGDASGWTRGLMMSSLMG
                     VRMGQILGKKTN"
BASE COUNT         2182 a         1524 c         1094 g         2424 t
ORIGIN      
        1 cattatatga actgtcatcc tgattgtcag cgtttgtgct aatattggtt aaacacgggt
       61 gttacgttgc tgtcttcaaa atataatcct gcttgctcga aagacaatcc ttcaatattg
      121 aactcaacat cgcttatgcc atactgctct aaacaaagca ctactattga attcatatcc
      181 ttgtagtctt cttccgacaa attaacagct tcaacaccta gattaactac tgcactatcc
      241 ccgataactt ttacatccct taaattaata cccacaggta tattgttgct gagagttgta
      301 tctgcaggag gtccttcaaa caatttttcc ataacaaatt ctacaggatt gcccacctta
      361 gctgctgaaa atgttattgg tacataatgc cccattactt cagtatcggg agccttgtaa
      421 taaacggtat aatttacacc atctgccgag ccgtataaat taatattttc tctttcaaat
      481 cccttattga ttgcataccc gttggtaagg gaagacaatg cctgcccgtc taccaataat
      541 tcaaccttat caatggttgg gaattcagta agcgcatatg taattgcaga aatcaaattt
      601 tcttcctgtt ttaaggtaga agtatttaaa atatttttgc tgaagtccac tctgcatacg
      661 ccgtccttga tagacatgcc ccttatttcg gtaccttcgg gaagtacgcc gcgtagtcct
      721 gattgtgcaa ttcttttttc agtttcactt cctctaacca tacttctaag tgttgctttt
      781 gcaattcctt cttcccacgg aatttttgtg tttacaggaa ccacaaagcc gtattcatct
      841 tcataatatg ccattatgtt tgtcatttta gcttcttcgg tagaaattgt aacagtttct
      901 atgacttctt catatggttc ttctaatgct tcttctccaa ttgtgtcttc aacgatttca
      961 aaatctgtca aatcagacaa atcggctata tccggattat tttcgtcctt cagacccacc
     1021 atcatatcta aggtgccgca tgcagttaag catactgcaa taaaaataat cagtatcaga
     1081 gatattattt tattatgagc caatttcata ccctcctcac gccttatata tattattata
     1141 tttagcatgt accgaaagta tgactttaaa aaattatttc atcattatta ttcctgacat
     1201 gagacccctt tttccttttt gacctctgtg ggaaaagaac aaatcattgc ggcatttggt
     1261 acacatacct gcaacctcta tgttttcttc tttcattcct tctcttagca tattatattt
     1321 attgattccc cacaaatcaa aatgatactt ttctcctttt tttatcataa aatctttgta
     1381 tttaatatct gtgtttataa acatatctgc aacatcctta tcaacttcaa aacaacactg
     1441 ccccaaagaa ggacttatgg ctgcctttaa gtctgaagga ttgcccccaa gatcttttaa
     1501 tgccttaatc attttccccg taatatttgt cattgttcct ttccatcctg catgacttag
     1561 tccgattatt tttttaaccg ggtcgtaaaa gaatactgca ttgcaatctg catgaaaaga
     1621 catcaatgca atatttttcc tgtctgtata aagaccgtct atatcagtgt atttagcttt
     1681 cttcataact ctgcccttat cttcttccgt tacatatctg atattatcgg tatgagattg
     1741 aaatgttttg acaatatcct ctaaattaat atttaggcat tcagacaata tataataatt
     1801 ttcccttaca tcttcttcat catcgcctgt attaaaacct aaattcatgg attcaaaatc
     1861 acctttgctg actccgcctt ttcttgtggt aaagcagtga aaaagttcat catatccatc
     1921 aaaaatcgga aaagtcaaat attcaaaatc ttttatttta tttaatttat acataataat
     1981 tcctctcttc aagttcatta tggaattcat atactatgta aaataataaa atatccgctc
     2041 tcggtttcgg ccacgaataa ttctaaagct cattccggct aaaaatccta cagcttgcaa
     2101 actcgctccg ctcaaacagt gcaagctgct taacgaattt ttaacctgca ttcgctaaga
     2161 attataatcg tgtcctgcaa atgttcgcta catattttat tatttttctc tttattcaag
     2221 aaaacatttc atttattata ccatacctgt ataaaatttt tatatgattt tatataaaca
     2281 tatgttaaaa taattgtgta cctttatatt tagctaaaat aataaaaatc aaccaagaca
     2341 gccaaggagg ccatatgatt atttcgcctg tttttgataa ggagagcagc aaaccgttat
     2401 acatacaatt atatgaatat attaagacag aaataacttc cggtttatta aagcctgata
     2461 cgaagctgcc ttcaatcagg gaagcttcct ccacattaaa attgagcaaa acaactgtag
     2521 aaaatgcata cagccaatta gtggcagaag gttatataga taattatccg aaaaagggat
     2581 actttgtatg cgatttgagc gaatataatt ttgatattga agcaaccaaa aatacagtgg
     2641 aatactatga cgaaataata tatttaaatg atggcgttga taaagaaagc tttgacattt
     2701 caacttggaa aagactttac aacaaggtga tttctgatag taaaacaaat atttacaaca
     2761 gctgttcaac tcagggtgag gaatttttaa gagaagaaat tgcaaatttc gtaaatacga
     2821 tgcgcggcgg tcatacagat tcgcgtcagg tgattgtggg tgcgggtgtt caatatttat
     2881 tgggaatgtt gataggaatt ataaaagagg attacaatac tgttgcaatt gaaaaaccgg
     2941 gatttaaaaa agcagaattt gtttttgaag actacatgtt caacataaga catattgaaa
     3001 ttactgatga cggctttcca attaacgagc taaggaaaag tgatgctgaa gtaatataca
     3061 tcagtccctc tcaccaatat cctcttggaa ccataatgcc tgtcaataaa cgaatggaat
     3121 tgctggattg ggcttacaag aaaaacgcac tcattatcga ggatgattat gattccttga
     3181 ttaggtatgg aggtgcgccc ataccaagtc ttcaaggact tgacaaaaat gattgtgtag
     3241 tctacttagg ctcattttca aaaatgctgc tcccatctct aagaatcagc ttcatggtaa
     3301 taccaaaaaa acttctgcct aagtataatg aaataaagga ccgctataca caatcaactt
     3361 caaaaatcga ccagctagtt cttgcaaact ttatgaaaga cggatatatg gttaaacacc
     3421 tccgtaaaat taaacgtata tataaaaaga agaattctta tctaacatca aattttagaa
     3481 acctttttgg agatagggca gaagttataa gttctgattc cggtcttcat attgtttgtg
     3541 aagccaatac aaagaaggat gcgcagcaaa taataaaaga tgcgcaaaac tcacgcatcc
     3601 ttataaacat tatccgaatc aagaaaaaca aggttatgtt ttccttaaac tacagcggtg
     3661 tggacttaaa taaaatacat gaattcacca tcctattagg aaaagtgttg ttcgaatgag
     3721 aaattcgctg tcgtcaggat aatcttgtcc taatatctag agtgttgaga ttcttcgttt
     3781 acgttcagaa ttacttaggg gcatctcgtt cttaaacaaa tccttgtaca attcgcctac
     3841 caacaagggt atgaatgaaa atgccgttac aatctgccaa tccctaagag taagagggac
     3901 tacatcaaat atttcattca attgaggtat gtataaaacc acaaacgtaa gcatgaagga
     3961 tacaaagacc gcctgaatca ttcttatgtt agtaaaaagt cctattttaa acaaggtata
     4021 attctgagac cttgaagaaa aagctctcaa caattctgac aaaatcaagg ttgtaaaaac
     4081 taccgagcgt acgtgtatta agccgcttcc ggtaccgtac atcctcattg cccaataata
     4141 cgcaaagagc gaagcgaatg atatggcaat tgcctggaat acaattcctc caagcatttc
     4201 tccatcaagt attccctcct ttgtacttct gggaggctgt ttcataatac ccggttctgc
     4261 attttcaacc cctaatgcca tggcaggaag gctgtctgta acaagattca gccacaaaag
     4321 ctgtatcgga agaagtggaa cattccaacc caatagaata cttataaaca cgagaaggat
     4381 ttcacctata ttgcagctta ataaaaagaa tacaaatttc ttaatattag aaaaaattat
     4441 tcttccttct tccacggcat ttactatggt tgcaaaatta tcatccgtaa gtataacttc
     4501 tgcagtgttt tttgccacat ccgtaccggt aattcccata gcaacgccga tgtccgcctt
     4561 tttaagagca agagcgtcat tgactccatc ccccgtcata gatgtaatat gtccgttttc
     4621 ctttaaagca gttactattt taactttgtg ctcaggtgat actctggcat aaactttggt
     4681 tttctttaca acttccctta attcttcatc tgaatattta tctaactcct cacccattat
     4741 tgcctgtcct tcattattgg ccattcccaa ttcctttgca atggcatatg ctgtttcctt
     4801 gtaatcccct gtaatcatta ctgtttcaat tcccgcttgt ctgcaatgct taatggaatc
     4861 ttttacctca ggtctgggag ggtcaatcat tccgactaaa cccacaaata tcatgtcatt
     4921 ttcaacctgt tcaaatgtga gatgctcagg cagtctgtcc cataccttgt atgcggctga
     4981 caaaactctt agggctgacc gtgcaaactt agtatttaca gatcttacct tatcttttaa
     5041 ctctttgttg aaatcaacga ttctgccgtt aagtgcaatt ttactgcatt tatttataac
     5101 tatatcggga gcaccctttg tgaatgaaac aatttttcct tcaatatagt ttgagtggaa
     5161 agttgtcatc atcttccttc ccgaatcaaa gggaagctcg gcaattctcg gataaatcat
     5221 attaagctgc tctgatgttt gtcctaactt tcccgcaaaa gctactattg ctccttcagt
     5281 agggtcaccc attactttat aaaagcctga tgaattatca agttttgcat catttgctaa
     5341 aacacctacg gatattaaat ttcttaaatt aggaagcgaa cttataccta tactccttcc
     5401 gttgagcttc acatctcctt ttggctcgta tccgatacct tcaacataca aaattctgtc
     5461 atcaacatat gctttcacaa ctgtcatttc attttgtgtc aaggtaccgg tcttgtctga
     5521 acatattaca gatgtagctc ccagtgtttc aaccgccaga agttttttta caattgcatt
     5581 tttttctgcc attctgttca ttccaatggc taatacaatt gtaacaattg caggcaaccc
     5641 ctccggtatt gctgctaccg caagacttat tgatgtcaaa agcatattta agggttgtct
     5701 tccttgaagc atgcccacta taaatactat tatgcaaata acaattgtca gtgttccaag
     5761 aaccttgcca agctgattta actttatttg caggggagtt tcttcttctt cgtaagtttg
     5821 tatttttaca gcaatattac cgatttccgt attgtgtccc gtagatgtta ctacaccctt
     5881 gcccctaccg taggttacga ttgtgctgga gaagcccata ttatgcctgt cccctattcc
     5941 gacggtacca actatttttg cttttgcatc cttctccaca ggaactgatt ctcctgttaa
     6001 agaagcttct tcaaccttta aatttgagct ttcaaagagt cttatgtcag caggaattat
     6061 atctcctgcc tccaacacta ctacatctcc cggaacaatg tctgcagcag gaaccacggt
     6121 taaattgcct tcccttataa cctttgcatt gggtgcgcta agcctttgca aagcttctac
     6181 cgacttttcc gcctttccct cctgatagat tccaagagca gcatttaaaa ctacaattgc
     6241 caaaattatt acagcgtctt ctctttcccc tacaaacgca gatactcctg ctgctatgag
     6301 caggataatg attaagaaat ctgcaaactg agctgcaaac ttatttaata tacttttttt
     6361 cgcctgttct tcaaattcat ttttgccata cttttcaatt cttcttttaa cttccttttc
     6421 acttaaccca acacttaaat ttgtgtctag ttcttttaaa acttcatttt tgcctttgtt
     6481 ataccattcc aaattatttt tcctcctgta ataatttgtg ttgtcaacac tagtataata
     6541 tattggcaaa tttataattt attccaaatt tatggacaat ccttaatgat tgcccaatga
     6601 attttttcaa aaaaataagt tggggacact cctcttactt caaagattta ccctatagta
     6661 gaagtgtgtc cccatacctg ttttaatttg tccccatatg ctattaattt gttttcttcc
     6721 ctaatatctg tcccattcgt actcccatca atgatgacat catcaaacct ctcgtccagc
     6781 cgcttgcatc acccaaacaa tataaatttt tgatatttgt ttcaaattca gtacttattt
     6841 caaccttgtt actgtaaaat tttacttcag gcgcgtaaat aagagtttct gttccggcaa
     6901 atcccggaac cacaacattt aatgcttcta taaaattcaa aatattcgtc atggctctat
     6961 atggcatagc tgaagtaata tctcccgcta ccgcgtccgg caatgtcggt ctgacatttg
     7021 accttgacag ttcatgatcc catgttcttt tgccgtccag tatatcaccg tatctttgaa
     7081 ccaaaatttt tccgtctcca agcatgttta aaagctctgc aacctttgca ccatacttaa
     7141 tcggctcatt aaaaggattc ctgaaattat gcgagctcaa aattgctaaa tttgtattat
     7201 ttgttttcct atccttatac gaat
//
LOCUS       DAOSOW010000042         7164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_10478_length_7164_cov_4.155155, whole genome shotgun sequence.
ACCESSION   DAOSOW010000042 DAOSOW010000000
VERSION     DAOSOW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10478_length_7164_cov_4.155155"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..2694
                     /locus_tag="PLD67_01980"
     CDS             91..2694
                     /locus_tag="PLD67_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HOT21328.1"
                     /translation="MQSYKNLLAHREIKNGFVKEQPLWEHLIATARKCSEVGALIEIK
                     AVCALIGLLHDFGKYIDLFQLYIKGEYKGNVSHSSAGAKVLEYIEEKVMHEYNISALL
                     KGRGLNDKVWRLYREIMQYPILAHHGLYDIIDLDFNYHTGLRLDYDKDNKYNFNMEGL
                     KFLEFLNDEYEALTDKSIYGIYYEGFIEFLALYDKLMIIARLTSEDLKIRRKARNFYY
                     GALVRLLLSILKDADIYDSTNCFNEHKDKLYSNEEVAVMWESMSRNVEALYKNFKGKS
                     NKSQLDILRTELADEIYEFSKKHEKGAYKLSLPVGSGKTLASLRYSVANADEFHKLRI
                     FYFTAFLTVLEQNAYAIKKALGEDNKEHILEHHSNVIDEKHVDSKNESDKDQEEYLIT
                     EYFKESWEPPFILSTMVQLSNTLFKDRASNIRRFSKLINSVIVIDEIQSLPTKAVYNF
                     NLMTNFLTHIMNCNILHCTATQPNFDSKSALKYPCIYGDNLDNPESAIIESVNILEVF
                     DRVEYFSLLGDDLIKTLDAKELSDHIKDQLKSEKSALVVLNTKNAVLEFYNTLIEDED
                     IIKLDCEVIYLTTNQCPKHRLNIIDGMKKRLKALRDFKDDHKLICISTKLVEAGVDID
                     FDLVYRSVTGLDSVVQCGGRCNREGRKSGKGKLFIFEYEGENLSNLPEFEEQRRAAKT
                     ALRVLKSKITCGSKINIEDACDYYFEKFFSNHEFQGSHLEFIIKKFNRNMDVEIDTII
                     NLLTTNPNESENYKTKNGDKPDFILKQSFKTAAQEFNLINEEGVSVVVEYENKQILEE
                     LYKAIYEKKYDVAKRVIRELQPYTIHVRRIDDIKSYVRIELDGKLFILDKNYYDAKIG
                     LKVKELQLLMF"
     gene            2706..3482
                     /gene="cas5c"
                     /locus_tag="PLD67_01985"
     CDS             2706..3482
                     /gene="cas5c"
                     /locus_tag="PLD67_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007892704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HOT21329.1"
                     /translation="MSDFRSEPFYMKVCGDYALFTDPMTKGGGEKFSYQVPTYQALKG
                     ISEACYWKPTFYYIIDSVKVMKKIRTETKSILTLLKNGEKDLSYYTYLRDVEYLVKFH
                     FAWNENRCDLEYDRNEKKHEQILLRSMARGGRRDVFLGTRECIGYIMRIKERDYEEAK
                     SCYYGEKISMGIMFHSFSYPDETYRGSNENVLVSNFCSIVMEDGIIQFKEPKDCEIKH
                     MLGNYSIKKFNKENFTTADEVLLEYERIGVSDNELDVRSS"
     gene            3457..5481
                     /locus_tag="PLD67_01990"
     CDS             3457..5481
                     /locus_tag="PLD67_01990"
                     /inference="COORDINATES: protein motif:HMM:NF021243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOT21330.1"
                     /translation="MSLMSALLETYDFALERGLVDCTMIDNKCPVLLPVYHSSKKSGG
                     EDIFEITIDKNSVAVDGKFLGKDERVVFPITEDSIIRSGSKIAPHAVCDELSYLSREI
                     DPLKNEEYIKGIEELLKYEKANNCESFKIIGNYIIKNTILVDFLKFCLKSKKYTIDDK
                     FKVIIELENTGEKAVSKTLDLNKIFITFKIERENEGDISLTNDKDIHKFYIGYVKSKN
                     SSRELSYCDITGKLDYCVERHRGIIGNAKLISISNNDETYYGRFKSGGDIYHISYETS
                     QKVHNMLKYLVDDTRYSSFIGEGAYVINWLSNELEKGGIKLLSEFKEGDASVKDGSWD
                     EGIDEDEDGITDEDEATMTELGGGISRRIGAYFLGKGGEIDFEGDFYVLIIEKISNGR
                     ISIKHFRRLSRSDAYERLKYWYKTIEWKLGKHLKTPSVYQIVDFLYGYENDKGFLKCE
                     KNALRRSQIERLIPCLTDSKRIPRDMVERACSRLAKNQSYKKSWDIALYIGCSLIKKY
                     KNDYCDYFIDVENIGEVNQLKESISFNYGRLLAIYDKVELDAVWSRNKGFNEDGLQIK
                     EDKNSKDKKEYIRVTNASRMYNSMTRMPEKTLSILSKKLNPYFDILRKKNGGAYAFYD
                     KEITKINAAIKELKESNGYKESPVDKDFYVGFYYQKNLLYNKGMSSGDKQ"
     gene            5534..6391
                     /gene="cas7c"
                     /locus_tag="PLD67_01995"
     CDS             5534..6391
                     /gene="cas7c"
                     /locus_tag="PLD67_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HOT21331.1"
                     /translation="MSTLDKKIDFTVFFTVRNANPNGDPLMDNMPRTDYEGYGEVSDV
                     CIKRKIRNRLQEMGNDIFVQSQDRIQDGHKSLEARFDAEFNRNDGDEEVAKRACMKWI
                     DVRSFGQVITYQKRSLGIRGPVSISLAKSLSEVEAISMQITRSTNGMEAEEGKSRSKD
                     TMGLKNYIDFGVYKFTGSINCYFAEKTGFTDEDAEQIKEALRTLFINDMSSARPEGSM
                     EVKKIFWFTHPNKLGVASSAKINSIVKNILPDKQKITSYEEYSIGIDKEKYEEYKKAG
                     LIIEELEGI"
     gene            6410..7084
                     /gene="cas4"
                     /locus_tag="PLD67_02000"
     CDS             6410..7084
                     /gene="cas4"
                     /locus_tag="PLD67_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOT21332.1"
                     /translation="MAVKDNKEEDYLLISGIQHFVFCKRQWALIHIEQQWQENLRTIE
                     GEILHEKTHDNTVKESRGDIIISRGMGIFSTTLGLTGACDAVEFHRSLEGINIFGRDG
                     TFKPIPIEYKKGKPKEDESDVLQLCAQAICLEEMLLCEIPEAFLFYGETRRRLKIVLD
                     EVLRDRVKAITKEMHELYDRRYTPKVKPSKSCKACSLTEICIPKLCKNPSTAYYINKN
                     LSEVEE"
BASE COUNT         2620 a         1015 c         1577 g         1952 t
ORIGIN      
        1 ttcttataaa tacacaatat aatttaaata cacactttat taagtgacat aagcataata
       61 gagatacgaa aagaaagaag ggattataaa ttgcagtcat ataaaaattt attggctcac
      121 cgtgagatta aaaacggttt tgttaaagag cagcccttgt gggagcattt aattgccaca
      181 gcccgtaaat gcagtgaggt gggtgcttta atagaaataa aagcagtttg tgcactaata
      241 ggccttttac atgattttgg taagtacata gatttattcc aattatatat caagggtgaa
      301 tataaaggta atgtaagtca ttcttcagca ggagcaaagg tgctagagta tattgaggag
      361 aaagtaatgc atgaatataa tatatcagca cttttgaaag gcagggggct taatgataaa
      421 gtctggcgct tatataggga aataatgcag tatcccatat tagcccacca tggattatat
      481 gacataatag atctggattt taactaccat acgggactga ggcttgatta tgacaaagac
      541 aataaatata actttaatat ggaaggcctt aagttcctag agtttctaaa tgacgaatat
      601 gaggcattga cagacaaaag tatatatggg atttactatg aaggcttcat cgaattttta
      661 gctctgtatg ataaattaat gattatagcc cggctaacat cagaagactt aaagataagg
      721 agaaaggctc ggaactttta ttacggagcg ttagtaaggc ttttgctttc tatcttgaag
      781 gacgcagaca tatatgattc gaccaattgc tttaacgagc acaaggataa gctttattcc
      841 aatgaggaag tggccgtaat gtgggaaagc atgagcagga atgtagaagc cttatataaa
      901 aatttcaagg gcaagtctaa taaatcccaa ttggatattt taagaacaga gctagcggat
      961 gaaatatatg agttttcaaa aaaacatgaa aagggagctt acaagcttag ccttcctgtg
     1021 ggctccggca agacattggc ttctctgaga tattctgttg caaatgcgga cgaatttcac
     1081 aaattaagga ttttttattt tacagccttt ttaacagtac ttgagcagaa tgcttatgcg
     1141 ataaaaaagg ctctaggaga ggataacaag gagcatatat tagagcatca tagcaatgtt
     1201 attgatgaga agcatgtcga tagtaaaaat gaaagcgata aagaccaaga ggaatacctt
     1261 attaccgagt attttaagga aagctgggag ccgccattta tactatctac catggtgcag
     1321 ctttcaaata ccttgtttaa ggacagagct tctaacatta gaaggttttc caagctgata
     1381 aattcagtta ttgtaatcga tgagatacaa agcctaccca caaaagccgt ttataatttc
     1441 aacctgatga ctaatttttt aactcatatc atgaactgca acattctaca ttgcacagcg
     1501 acacaaccta attttgatag taagagcgca ctaaaatatc catgcatcta tggagataat
     1561 ttagacaatc ctgaatcagc aataatagaa tcagtaaata ttttagaggt ttttgataga
     1621 gtggagtatt ttagcctgct aggtgatgat ttaatcaaga ctttagatgc aaaagaactc
     1681 tcagatcata taaaagacca acttaaaagt gaaaagagcg cattggttgt gcttaacaca
     1741 aaaaatgctg tcttagagtt ttacaatacc ttaattgagg atgaggatat aataaaatta
     1801 gactgtgagg ttatatatct taccacaaat caatgtccta aacacaggct taatataata
     1861 gatggcatga aaaaaaggct taaggcttta agagacttta aggatgacca caagctcata
     1921 tgcataagca caaagctagt ggaagccggc gttgacatag attttgacct ggtttacaga
     1981 tctgtcacag gtcttgatag cgtcgtacaa tgcggaggac gctgtaatag ggaaggcaga
     2041 aaatctggaa agggaaagct ttttatattt gagtatgagg gggaaaacct tagcaacctt
     2101 cctgagtttg aagagcagag aagagcggct aagacggctt tgagggtttt aaagagcaaa
     2161 ataacctgtg gatcaaaaat aaatattgaa gatgcctgtg actattattt tgaaaagttt
     2221 ttttccaatc atgaatttca agggagccat ttagagttta ttattaagaa attcaataga
     2281 aatatggatg ttgagataga caccataata aacttgctta caacaaatcc caatgagtcg
     2341 gaaaattata aaacaaaaaa tggagataag cctgatttta ttcttaaaca aagctttaaa
     2401 acagctgctc aggaatttaa tttaattaat gaggagggag ttagtgttgt tgtagaatat
     2461 gagaataaac aaattctaga agaattgtat aaagctatat atgaaaaaaa gtatgatgtc
     2521 gctaaaagag ttataagaga gcttcagccc tatacgatac atgtaaggag gattgatgat
     2581 attaaatctt atgtaagaat agaacttgat ggcaagctgt ttattttgga taaaaattat
     2641 tatgacgcaa aaattggcct gaaagttaag gaactacaat tactaatgtt ttgaaaggag
     2701 gagccatgag tgattttaga tctgaaccgt tttatatgaa agtttgcggc gattatgcat
     2761 tgtttacgga tcctatgaca aagggaggcg gtgagaagtt tagttatcag gtgcccacat
     2821 accaggcgct taaaggtatt tcagaagctt gttattggaa gcctacattt tattacatta
     2881 ttgattcagt aaaagtcatg aagaaaataa ggacagaaac aaagagcata cttactctat
     2941 tgaaaaacgg tgaaaaggac cttagttatt acacatattt aagggatgtg gagtacctgg
     3001 tcaaattcca tttcgcgtgg aacgaaaatc gttgtgactt agaatacgac cgtaatgaaa
     3061 aaaagcatga gcagatactg ttaagaagca tggcaagggg ggggagaaga gacgtttttc
     3121 tgggaaccag agaatgcatc ggttacataa tgcgaattaa ggaaagagac tatgaggaag
     3181 caaaaagctg ttactacggg gaaaaaatat caatgggcat tatgtttcac tctttttcct
     3241 atccagacga gacctataga ggcagtaatg aaaatgtatt agtatcaaat ttctgctcca
     3301 tagttatgga agatgggatt atacagttta aggagccgaa ggactgtgaa atcaagcata
     3361 tgttaggaaa ttactctatc aaaaaattta acaaggaaaa tttcacgact gctgacgagg
     3421 tccttttaga atacgaaagg ataggggtga gcgataatga gcttgatgtc cgctcttctt
     3481 gaaacatatg attttgccct tgaaagagga ttggtggact gcactatgat tgacaacaaa
     3541 tgcccggttc tacttcctgt ataccattca agcaagaaaa gcggcggcga ggatatattt
     3601 gagataacaa tagataaaaa tagcgttgct gtagacggta aatttttggg taaggatgaa
     3661 agggtggtat tccctataac agaggattcc ataataagat ctggctctaa aatagctccc
     3721 catgctgtgt gtgacgaact ttcgtatttg tctagggaaa ttgacccttt aaagaatgag
     3781 gaatatataa aaggcattga agaattgctt aaatacgaaa aagcaaataa ctgtgaaagc
     3841 tttaagataa taggcaatta cataataaaa aatacaatac ttgtggactt tttaaaattt
     3901 tgcttaaaga gcaaaaaata cactatagac gataaattta aggttatcat cgagcttgag
     3961 aataccggag aaaaagcagt cagtaagact ctggacttaa ataagatatt tataacgttc
     4021 aagatcgaaa gagaaaatga aggggatata tctttaacaa atgataagga tatccacaag
     4081 ttttatatag gttatgtcaa aagtaaaaac agttccagag agctatccta ctgtgatatt
     4141 acaggtaagt tagactattg tgtggaaagg cataggggaa ttatagggaa cgcaaagctt
     4201 ataagcatca gcaacaatga tgaaacttat tatggaaggt ttaaaagcgg aggtgatata
     4261 taccatataa gctatgaaac ctcccagaag gttcataaca tgcttaaata ccttgtggat
     4321 gatacaagat actcaagctt cataggggaa ggggcttatg taataaactg gctttctaat
     4381 gagttggaaa agggtggaat caagcttttg tcggagttta aggagggtga tgcttcagtt
     4441 aaggatggat cttgggatga aggaattgac gaagacgaag acgggattac cgatgaagac
     4501 gaagcaacaa tgacggaatt aggaggcgga atttctagaa gaataggtgc ttacttttta
     4561 ggtaagggag gggaaattga ctttgaggga gacttctatg tattgataat agaaaaaata
     4621 agcaacggga ggatttccat aaagcatttt agaaggcttt caaggtctga tgcgtatgag
     4681 aggcttaaat attggtacaa aaccattgaa tggaaattgg gtaagcattt aaaaacaccg
     4741 tcggtgtatc agatagtaga ctttttatat ggttatgaaa acgacaaagg gttcttaaaa
     4801 tgcgaaaaaa atgccctaag acgaagccag atagaaaggc ttattccatg cctgacagac
     4861 tcgaaaagaa ttccaaggga tatggtcgag cgtgcttgca gcagacttgc aaaaaatcag
     4921 tcatacaaga aaagctggga tattgccctg tatataggct gctctctgat aaagaaatac
     4981 aaaaatgatt actgtgacta ttttatagat gtggaaaata taggggaggt gaatcaattg
     5041 aaagaaagta taagctttaa ttatggcagg cttttggcta tatatgacaa agtagagctg
     5101 gatgctgtct ggagccggaa taaaggtttt aatgaagacg gattacaaat aaaagaagat
     5161 aaaaatagca aagacaagaa ggagtatata agagttacca acgccagcag gatgtataac
     5221 tccatgacaa gaatgccgga gaagacgctt tcaattttga gcaagaaatt aaatccctac
     5281 tttgatattc ttagaaaaaa gaatggagga gcatatgctt tttatgacaa agaaataaca
     5341 aaaattaatg cagcaattaa ggagcttaaa gaatcaaatg gttataagga aagccctgtt
     5401 gacaaagact tctatgtagg cttctattac caaaaaaatt tactttacaa caaaggcatg
     5461 tcatcaggag ataaacagta aaaaagatag caagaagtaa ttcaatataa tgattataag
     5521 gaggatataa agaatgagta ctttagataa gaagattgat tttacagtat tttttactgt
     5581 gagaaatgcg aatcccaatg gagatccttt aatggacaat atgccaagaa cagattatga
     5641 aggctatgga gaagtatcgg acgtttgcat taagcgtaaa attaggaaca gacttcaaga
     5701 gatgggaaac gatatatttg tccaatctca ggacaggatt caggacggac acaaatcatt
     5761 ggaagcaagg tttgacgcag aatttaatag aaatgacggt gatgaggaag tagccaaaag
     5821 ggcctgtatg aaatggatag atgtaaggag ttttggccag gtaattacat atcagaaaag
     5881 gtcgcttggc ataagaggcc ctgtatccat aagccttgct aaaagcctca gcgaggtaga
     5941 ggctataagc atgcagataa ccagaagcac aaacggcatg gaggcagaag aggggaagtc
     6001 taggtcaaag gacacaatgg ggcttaaaaa ttatattgat tttggagtat ataaatttac
     6061 aggaagcata aactgctatt ttgcagaaaa aacaggtttt acagatgagg atgcggaaca
     6121 aatcaaggag gcattaagaa cactatttat aaatgatatg tcctctgcga gacccgaagg
     6181 aagcatggag gttaaaaaga ttttctggtt tactcatcct aataaactag gagttgcatc
     6241 atcagctaag attaattcca ttgttaaaaa tattcttcct gataaacaaa agatcacgtc
     6301 ttatgaagaa tatagtattg gaatagacaa agaaaaatat gaggagtata aaaaagcagg
     6361 cttaattata gaagaattgg aaggtatcta atagatggat ctctctggaa tggctgttaa
     6421 agataataaa gaggaagatt atttattaat atcaggaata cagcactttg tattctgtaa
     6481 acgccagtgg gcattgatac atattgagca gcagtggcag gaaaacctgc gtaccattga
     6541 gggagaaatt ttgcatgaga aaacccatga caatactgta aaggaatccc gtggggatat
     6601 tattatttct cggggtatgg gaattttctc aactacttta ggcttgaccg gagcttgtga
     6661 tgctgttgaa tttcataggt cattggaagg gataaacata tttggcagag atgggacatt
     6721 taagccaatt cctatagagt ataaaaaggg aaaaccaaaa gaagacgagt ccgatgtact
     6781 gcagctatgt gcccaggcta tatgtctcga agaaatgctt ttatgcgaaa tcccggaagc
     6841 ctttttattc tatggagaaa ccagacgcag gttaaaaatt gtactcgatg aagtgctgcg
     6901 tgatcgagtt aaagcaatta ccaaagaaat gcatgaatta tatgatagga ggtacacacc
     6961 taaagtaaag ccgtctaaaa gctgcaaagc gtgttctctt actgaaattt gcataccgaa
     7021 gctatgcaaa aatccttcca ctgcctatta tatcaataaa aacctatcgg aggtggagga
     7081 atgaggaaac tgttgaatac actctatgtc acatctccgg atacttattt atcacttgac
     7141 ggagaaaaca ttgtgattct gaaa
//
LOCUS       DAOSOW010000043         7108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_10584_length_7108_cov_4.615766, whole genome shotgun sequence.
ACCESSION   DAOSOW010000043 DAOSOW010000000
VERSION     DAOSOW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10584_length_7108_cov_4.615766"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..439)
                     /locus_tag="PLD67_02005"
     CDS             complement(62..439)
                     /locus_tag="PLD67_02005"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOT21333.1"
                     /translation="MKNGISKELAAYLIAGILTTIVNFIVYYAMLFIGIDYKISNTAA
                     FIISVIFAFIINKKYVFLSDKSYFKEFIKFSLGRLFTYALDIGAMIILIEVFSVSEYM
                     AKLWTNILVMISNYLISKFWTFK"
     gene            complement(439..1392)
                     /locus_tag="PLD67_02010"
     CDS             complement(439..1392)
                     /locus_tag="PLD67_02010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017153413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT21334.1"
                     /translation="MKKVSLLIPAYNEEETIPLLYNELNKVIDKISGYEFEILFVNDG
                     SSDNTLNILRDLRKRDSRVNYISFSRNFGKETAMAAGFDYVTGDATVILDADLQDPPE
                     LIEEMIFYWEKGYDDVYAKRRSREGETWLKKFTSAAFYKLLRKMTKIPIQEDTGDFRL
                     LDRRAIESLKKLREKQRYTKGMFSWIGFNKKEILFDRKPRAAGKTKWNYLKLFNLALE
                     GVTSFTTSPLRISTILGILVSIFSIVYMFIVLIKSLIWKDPVQGYPSMMVTILFLGGV
                     QLVCLGIIGEYVGRIFNETKYRPLYIVDEHNNEKIEDGGIL"
     gene            complement(1394..3619)
                     /locus_tag="PLD67_02015"
     CDS             complement(1394..3619)
                     /locus_tag="PLD67_02015"
                     /inference="COORDINATES: protein motif:HMM:NF040368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6273 domain-containing protein"
                     /protein_id="HOT21335.1"
                     /translation="MESIKRLGIFILIFAFSLVLLLKEPFIGIADNSDYYRVIQPLGF
                     KPEISNRYFYAYNFYTVNDMSSEDIKGSLSNIISPKVENDNEYFSTQFIFIKVSMIIN
                     YLLKIVLGKSPEIFNIKVLGILYAAIYSYGLCLFLTNINFKYKYINFLFLIIALVILC
                     DMGYLLYFNSFFGEAAIIASLMITLGLLTAIIKTESKIKSLFYVILFYIFALALTGAK
                     VANTPIGILIGIFSLALFIVKADWLSRAVILIGSILIICFSIFYYTNAPRWMSQVNNY
                     QSIFFGITKDSNEPEKDLEKLSIPLKYLPLTNTHGFLDHGEFDIYSDEFQKEVYDNAT
                     FLDILKFYFLNPSRAVEKLKLSADSSVIIRPSYLGNCSKEDEPERLSFTERFSLWSNI
                     RKNALGYAFYIIVSYSVLFFIINIYEIINNIKQYDYENTAFAFAALLLFLTTMSQFVL
                     PIIGNGEADLQKHMLLFNLCFDIMILVGICWLINNFYTKTVSAVVLTAFVVFCIAIFI
                     QTANEEIKETGTLKIGQYIYLGSYKNEPLKWVVLNKDENGYLLWCDNTVEYMEFDYSD
                     ETNSDNIYGSNNWIESDVRRWLFEFKSNFNDEEKLLIKDVKLKNILSYNNIEKSIGGN
                     KPFYWNSITSYVSQNYNTDAYYNYSAESVFLLDVYQLQKYVYENKISLKKQERYWLRT
                     PYYSSESMVRIVDKDGFVYHKDANVKAGVIPAVYIDENVSAIEGDGTYTSPIAIEKSR
                     R"
     gene            complement(3782..4849)
                     /gene="orr"
                     /locus_tag="PLD67_02020"
     CDS             complement(3782..4849)
                     /gene="orr"
                     /locus_tag="PLD67_02020"
                     /EC_number="5.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine racemase Orr"
                     /protein_id="HOT21336.1"
                     /translation="MYPRLVIDLKKVKNNLDRITEMVKGSGSSLMIVTKGYSADMEIF
                     KILDGSDIDYLADSRIQNLKKYEGAKKERVLLRLPMNSETDEVVKYADISLNSELKTI
                     KNLNESAKRQNKIHKILLMIDLGDLREGIFFKNEDEIYHTVEEVLKLNNIELFGLGVN
                     LTCYGAVIPKSDNLSILVEIARKIEKKFDIKLQMISGGNSSSIYLIGRKELPEGINNL
                     RVGEAFLLGGETAYSQKLEGFYDDAFTLEAEIVELKEKQSVPIGETGVDAFGNKPVYE
                     DRGIIKRAIIAVGRQDVDPDALHPIDSKIDILGASSDHLILDISKSDMKYKVGDIVNF
                     KLSYSSLLRATTSGYVDRVYK"
     gene            complement(4858..6210)
                     /locus_tag="PLD67_02025"
     CDS             complement(4858..6210)
                     /locus_tag="PLD67_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HOT21337.1"
                     /translation="MKVNVLVAEIGSTTTVVNAFNGIGTGSPAFVGQGQAPTTVSEGD
                     VTIGLKGAIEDLKGNLQADTLTWDDMLATSSAAGGLKMTVHGLVYDMTVRAAKEAALG
                     AGGIIKMITSGRLRKTDLKKIQDIKPNIILVAGGVDYGERDTALYNMEMILSMGLKIP
                     IIYAGNIENQEEVRLMCEEADNQIYIVENVYPKIDTLVVEPTRKIIQDAFEEHIIHAP
                     GMAKVRDLVKGPIIPTPGAVMEAAKVLREEIGDLIIFDVGGATTDLHSVTEGSSEINS
                     ILISPEPVAKRTVEGDLGVYVNVSHIVEKIGMDNLKKEFPDAEELLENYKPIPKTDRE
                     KQFVERLTKEAVHTSLERHAGKLRYFYTASGKKTVAEGKDLTAIQYIIGTGGSLTRLP
                     DKKDILESVKTLAKEHELYPTEAARVLIDEDYILSSLGVLSKSYLSDALYLMKNSLRM
                     "
     gene            complement(6210..>7108)
                     /locus_tag="PLD67_02030"
     CDS             complement(6210..>7108)
                     /locus_tag="PLD67_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOT21338.1"
                     /translation="RDEDYFAPVSVHYGYNNIPAEFKKASDAIGGDTFEDRSKIQYID
                     ELDEHDNVNVRLKEVEKYYDTNLVKPEVEWRADGIVLLTIFLPLDERTAEFAAIKCGE
                     KMGLEDVTVVHKQCIHPSEGTYCEVKGRVNFDIDINELVIPEKPDLLSEAEIREDIQN
                     NPMFVVAATVGEDEHSVGLKETLDIKHGGIEGFGIKYEYLGTSCPVEKLVDAAIETNA
                     DAILLSTIITHDDVHIKNMKKVHDLAIEKGIRDKVMIICGGTQVTNEIALEAGLDAGF
                     GRGTHGIDVASFLVKKRREKKA"
BASE COUNT         2122 a         1455 c         1028 g         2503 t
ORIGIN      
        1 tatgcgaccg gagggagctg atattttggt gaacgagact gcgagaattc tggagaattc
       61 cttatttaaa tgtccaaaat ttgcttatta aataattgct tatcattacc agtatatttg
      121 tccacaattt tgccatatat tcgcttacgg aaaaaacttc tatcaaaata atcatagctc
      181 ctatgtctaa ggcatatgta aacaatcttc ccaatgaaaa ttttataaat tctttaaaat
      241 atgatttgtc gcttagaaaa acatattttt tatttattat aaatgcaaag attactgaaa
      301 ttataaatgc agctgtatta gatattttgt agtcgatacc gataaaaagc attgcataat
      361 aaacaatgaa gtttacgatt gtggttagta ttcccgctat gaggtaagca gccaattcct
      421 ttgaaatccc attcttcatt acaatattcc gccgtcttca attttttcgt tgttgtgctc
      481 atctacaata taaaggggtc gatactttgt ttcattaaaa attctgccta catattcgcc
      541 gataattccc aaacacacta actgaactcc tcccaagaat aaaattgtaa ccatcattga
      601 aggatatcct tgtaccggat ctttccaaat taaactctta ataagaacaa taaacatgta
      661 aacaatgctg aaaattgata ccagaatacc taagatggtg gatattctta aaggtgatgt
      721 tgtaaatgaa gtaaccccct ccaaggccaa attaaaaagc ttcagataat tccatttagt
      781 cttgcctgct gctcttggtt tcctgtcaaa taatatttct tttttgttaa agcctatcca
      841 gctgaacatt cccttggtat acctttgttt ttcccgaagt ttctttaaag attcaattgc
      901 ccttctgtcc aaaagcctga aatcgcctgt atcttcttga ataggtattt tggtcatctt
      961 ccggagcagt ttataaaacg cggcagaagt aaatttttta agccacgttt ccccttccct
     1021 gcttcttctt tttgcatata catcatcata tcctttttcc caataaaaaa tcatttcctc
     1081 aataagctcg ggagggtcct gtaaatctgc atccagtatc accgttgcgt cacctgttac
     1141 ataatcaaat cctgcagcca tagccgtttc tttgccgaaa tttcttgaaa agctaatgta
     1201 atttaccctt gaatctcgtt tccgcaaatc tctgagaata tttaaagtgt tatcgctgct
     1261 gccgtcatta acaaaaagta tttcgaattc atagccggaa attttatcaa tcaccttatt
     1321 taattcatta tataataagg gtattgtttc ctcttcattg tatgcaggta tcaataagct
     1381 gacttttttc atattacctc cttgattttt ctatggcaat agggcttgta taagttccgt
     1441 caccctctat agcactgaca ttttcatcta tgtatactgc gggaatcacg cctgctttaa
     1501 catttgcatc cttgtggtaa acaaatccgt ccttatcgac tattctcacc atgctttcac
     1561 tgctgtaata cggagttcta agccaatacc tttcttgctt ttttaaactt atcttatttt
     1621 catatacata tttttgtagc tgatatacat caagtagaaa aacactttct gcggaataat
     1681 tgtaatatgc atcagtatta taattttgac tgacatatga agtaatagaa ttccagtaaa
     1741 aaggtttgtt gcctcctata cttttttcaa tattgttata gcttaatata ttttttagtt
     1801 taacatcctt tataagaagt ttttcttcat cgttaaaatt actcttaaat tcaaagagcc
     1861 atcttctcac atctgattca atccagttat ttgagccgta aatattatct gaatttgttt
     1921 catctgaata atcaaattcc atatattcaa ccgtattatc acaccataaa agatagccgt
     1981 tttcatcttt attcaaaact acccacttta gaggttcatt tttatagctg cctaaataaa
     2041 tatattgtcc tatttttaat gtccctgttt cttttatttc ttcgttagca gtctgaataa
     2101 atattgctat gcaaaacaca acaaaagccg ttagaacaac cgctgaaacc gtctttgtgt
     2161 agaaattgtt tataagccaa catatgccca ctaaaatcat tatatcaaag caaaggttga
     2221 acaaaagcat atgcttttgc aaatcagctt caccatttcc aataatagga agcacgaatt
     2281 ggctcatggt tgtcaaaaac aatagtagtg ctgcaaaagc aaatgcagtg ttttcataat
     2341 cgtactgttt tatattattg ataatttcgt atatatttat tataaaaaat aagactgaat
     2401 aagaaactat tatataaaag gcatagccca atgcattttt ccgaatattg ctccacaaag
     2461 aaaatctctc ggtaaatgac agccgttccg gttcatcctc ctttgaacag tttcctaaat
     2521 aagaaggtct tattattacc gagctgtcag ctgacagctt aagcttttca acggctcttg
     2581 aaggatttaa aaagtaaaat ttcagtatgt ccaaaaatgt tgcattatca tatacttcct
     2641 tttgaaattc atcgctgtaa atatcaaact ccccatggtc caaaaatcca tgtgtgtttg
     2701 taagaggaag gtattttaaa ggaattgaaa gcttttccaa atccttttcc ggctcatttg
     2761 aatcttttgt aataccgaag aaaatacttt gataattgtt aacctgactc atccatcgcg
     2821 gtgcattagt ataataaaaa atcgaaaaac aaattatcaa aatagagcct attaaaatta
     2881 ccgctctgct aagccaatct gcttttacaa taaacagggc aagtgaaaat atacctatca
     2941 gtatccctat tggagtgttt gcaaccttag caccggttag tgctaaggca aatatataaa
     3001 ataaaattac ataaaaaaga cttttaattt tactttcagt ctttataatt gcagtaagca
     3061 agcccaatgt tatcattaaa gaagcaataa tagctgcttc tccgaaaaaa gaattaaaat
     3121 atagcaaata tcccatatcg cacaaaatta caagagctat tatcaaaaat aaaaaattta
     3181 tgtatttata tttaaaattt atatttgtaa gaaataaaca tagtccgtaa gaataaattg
     3241 cagcataaag aataccaagt acttttatat taaatatttc cggcgacttt cctaatacaa
     3301 tttttaataa ataatttata atcattgata ctttaataaa aataaattgg gttgaaaaat
     3361 actcattgtc attttcaacc ttcggactga taatatttga caaagagcct tttatatctt
     3421 cacttgacat atcattgaca gtataaaaat tatatgcata aaaatatctg ttgcttattt
     3481 cgggcttaaa tcccaagggc tgaataactc tgtagtaatc gctgttgtcg gcaattccta
     3541 tgaaaggctc ttttaataac aagactaagg agaaagcaaa tattaatatg aaaatgccca
     3601 atctttttat actttccatt ttaactccaa cctaacgata tagtaatatt atggcataga
     3661 taattttttt tgtaaatata tatcgacata aaaaaagagc cttattgggg tgccaggcag
     3721 caaacttggt tgcctggcac cacgaaaatg gcaccgcgaa aagaaatcgc cctaaaattt
     3781 tctatttata tactctatct acatatccgc ttgttgttgc tcttaacaag cttgaatagc
     3841 tgagtttaaa gttaacaata tctcctactt tatatttcat gtccgacttt gaaatatcga
     3901 gtatcaagtg gtcagagctt gctcccagaa tatctatttt agagtctatt gggtgcaagg
     3961 catcagggtc aacatcctgc ctccccaccg ctataattgc tcttttgatt atacctctgt
     4021 cttcatatac aggtttattg ccaaaagcat ctacgccggt ttcacctatg ggaacagatt
     4081 gtttttcttt taattcaact atttctgctt ccaaagtaaa tgcatcgtca taaaatcctt
     4141 caagcttctg gctgtaagca gtttcaccgc ctaaaaggaa agcctctcca actcttaaat
     4201 tattaattcc ttcgggtagt tctttccttc ctatcaaata tatggagctt gaatttcccc
     4261 cggatatcat ttgtaatttt atatcgaatt tcttttcaat ctttcttgca atttcaacga
     4321 gtattgaaag attatcactt tttggaatta ctgctccata acaggttagg tttacaccta
     4381 atccaaacaa ctcaatattg tttagcttta atacttcttc aacagtatga tatatttcgt
     4441 cttcgttttt aaagaatata ccttctctta agtctcccag gtcaatcatc agaagaattt
     4501 tatgaatttt gttttgtcgt tttgctgatt cgttcaagtt tttgattgtc tttaattccg
     4561 agtttagaga aatatctgca tatttcacta cctcatcagt ttcggaattc atgggcaatc
     4621 ttaataaaac tctttccttc ttagctcctt cgtatttttt gaggttttgg atgcgtgaat
     4681 ctgccaaata atctatatcc gagccatcta atattttgaa tatttccata tcggcggaat
     4741 aaccttttgt tacaatcatc agcgagctgc ctgagccttt caccatttcg gttattctat
     4801 caagattgtt ttttactttt tttaaatcga ttactaatct tggatacata ttatcaccta
     4861 cattcttaaa ctatttttca taaggtacaa agcatcactc aagtaacttt tcgacaatac
     4921 tccaagagaa gacaatatat aatcttcatc tattaaaact cttgcagctt ccgtcggata
     4981 caattcatgc tccttagcta aagtttttac tgattccaga atatctttct tgtccggtaa
     5041 tcttgtaagc gagccgccgg ttcctatgat gtattgaatt gcggttaaat cctttccttc
     5101 agcaacggtt tttttgcctg atgcagtata gaaatatctc aatttacccg catgcctctc
     5161 cagagacgta tgaactgctt cctttgtaag cctttcaaca aattgttttt ccctgtcggt
     5221 ttttggaatc ggcttataat tttccaggag ttcttctgca tcgggaaatt cttttttcaa
     5281 attatccatg cctatttttt caacaatgtg agaaacattc acatatactc caaggtctcc
     5341 ttcaaccgtt ctttttgcca caggctccgg actgattaat attgaattta tttcgctgga
     5401 gccctccgtt acggaatgaa gatctgtcgt tgctcctccc acatcaaata ttatcaagtc
     5461 tcctatttct tctcttaata cttttgcagc ttccattact gctcccggag taggaataat
     5521 aggtccctta actaggtctc ttaccttggc cataccgggc gcatgaataa tatgctcttc
     5581 aaaagcatcc tgaattattt ttcttgtagg ctctacaacc aatgtatcaa ttttgggata
     5641 tacattttct acaatataaa tctgattgtc cgcttcttcg cacatcaggc ggacctcttc
     5701 ctggttttca atgtttcccg cgtaaattat cggtattttt aaacccattg acaaaatcat
     5761 ttccatattg taaagagctg tatctctttc tccgtaatcc acaccgcctg caactaagat
     5821 tatgttgggt tttatatcct gtatcttttt taaatctgtt tttctgagcc tgcctgaagt
     5881 aatcatcttt atgattcctc cggctcctaa cgcagcttct ttagctgctc gaaccgtcat
     5941 atcatataca agcccgtgaa cggtcatttt caaaccgcct gcagcactac ttgtagccag
     6001 catatcatcc catgtcaatg tatccgcctg caggtttccc tttaagtctt caatagcgcc
     6061 cttaagcccg attgttacat caccctctga tacggttgta ggtgcctgac cctgccctac
     6121 aaaggcaggg ctaccggtac ctattccatt gaatgcatta actactgtag tggtgctgcc
     6181 aatttcagct accaatacat ttaccttcat tatgccttct tttcccttct tttctttact
     6241 aagaaactgg caacgtcaat accgtgggtt ccccttccaa aaccggcatc aagccctgct
     6301 tccaaagcta tttcatttgt aacctgtgtg ccgccgcaga taatcattac tttatctctg
     6361 ataccttttt ctattgctaa atcatgaact tttttcatat ttttaatatg aacatcgtca
     6421 tgagtaatta ttgtgcttag aagaatagca tcagcatttg tttctatggc tgcatcaact
     6481 aatttttcaa caggacatga agttccgaga tattcatatt taataccgaa tccttcaata
     6541 ccgccgtgtt ttatatccaa tgtttcttta agccctaccg agtgttcatc ctcgcctaca
     6601 gtagccgcaa caacaaacat cgggttattt tgaatatctt ctcttatttc tgcctctgac
     6661 aataagtcag gtttttcagg gataaccagc tcgtttatat caatatcaaa gtttactctg
     6721 ccttttactt cacaataagt tccttcggat ggatggatgc attgtttatg aacaaccgtc
     6781 acgtcttcca aacccatttt ttcaccgcat ttaatagcag caaattctgc cgttctctca
     6841 tctaatggaa ggaatatagt caacaatacg atgccgtctg ctctccattc cacctcaggt
     6901 ttaacaaggt ttgtatcata gtatttttca acttctttaa gtcttacatt tacattatca
     6961 tgctcatcca attcatcgat atactgaatt ttggagcggt cttcaaatgt atctccgcct
     7021 atggcatcac ttgccttttt aaattcagcg ggtatgttgt tgtaaccgta atgtacggat
     7081 acgggagcaa aataatcctc atctcttt
//
LOCUS       DAOSOW010000044         7082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_10654_length_7082_cov_4.436602, whole genome shotgun sequence.
ACCESSION   DAOSOW010000044 DAOSOW010000000
VERSION     DAOSOW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10654_length_7082_cov_4.436602"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PLD67_02035"
     CDS             <1..429
                     /locus_tag="PLD67_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-NAD kinase"
                     /protein_id="HOT21339.1"
                     /translation="DVFIGSKAIWNIDTIEKIIATRSHPASIGFSSLVGCKKIIYPED
                     DFGAYVDINSGSVRIKAPVAAGVVESVSVSNPVILRLEDEYEFTAKDRGTIALDGERE
                     IEFKKDQKLIFKITREGPYHVDVIKALETAQENNFFIIKN"
     gene            445..1743
                     /locus_tag="PLD67_02040"
     CDS             445..1743
                     /locus_tag="PLD67_02040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011949164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOT21340.1"
                     /translation="MAEFIVMPKMGLTMTKGMLTNWIKKEGDLVNKGDILFEVETDKI
                     TNKVEAKISGILRKILVDKETVDVFVPVGIIGRADEDISELLEQASRELKTDKPQKEE
                     GPKETRELSPAVQAGDRVKISPRAKKIAKDLKVDYNLVQGTGPGGAITEEDIRKFAEG
                     TKAKVSPTAAIVAEQLGVDIFNIEKDTRIMKADVIKYKLDESLAKYADPQEHRQSMTN
                     MRKIIAKRMLHSVQTSPSVSFNIKVDTTGMKELRDELKDTFKVSYTDILVKIVSEVLL
                     EFPLLNSTLDGDEIITRNYVNMGVAVALPAGLLVPVVKFTNIKGLKEISNEIKDLSER
                     AKTNDLLPDELTGGTFTISNLGMYGIESFTPIINQPEVAILGVNAIHKEPVVVNDEVV
                     IKPMMNLSLTADHRVVDGAVAAEFLAKLKKYIENPCLLLL"
     gene            1853..2758
                     /locus_tag="PLD67_02045"
     CDS             1853..2758
                     /locus_tag="PLD67_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HOT21341.1"
                     /translation="MELKNYQDLIDRIRKTDSKKRVAVAAAHDEHTLEAVFRAADDKL
                     VEPVLIGNKAKIIEILKELNVEYDLNSIISTDSDKESAEKTVELINSNMADFIMKGKL
                     QTADLLRAVVDKEKGLRTGRVMSHVAFLEIPAYHKLIAVTDGGMMMYPNAEEKKQILE
                     NVVDVFRAMGYECPKVAVLAAVETVNPKMPETVDADILKKMNQSGEIKDCLVEGPISV
                     DLTFNRESAEIKGFKSPVTGEADILLVPSITTGNIMCKALIEMAGATMAGMIVGAKVP
                     VVLASRGASSQEKYLSIVLSASSVK"
     gene            complement(2889..3317)
                     /locus_tag="PLD67_02050"
     CDS             complement(2889..3317)
                     /locus_tag="PLD67_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOT21342.1"
                     /translation="MKLRDIMSREVACLSAEDTVEKAAQLMKTYNVGSIPICAQNSVI
                     GIITDRDIALRAVAVGDDGYLQVKKVMTANPVVGTPDMDVEDAVRIMSENQIRRLPVV
                     ENNRLVGIVSLGDISVEPRLQDEAEIALKNISKSATNRFS"
     gene            3459..3950
                     /locus_tag="PLD67_02055"
     CDS             3459..3950
                     /locus_tag="PLD67_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOT21343.1"
                     /translation="MSIVKWNGRNLLKSIAENEKEATKLYRMFASEARIGEKFFELLA
                     KDEERHEKIYNALLEKYSDKLELEMEESDAEYMDLLVESNIGFDDELVEKAKKIFTKS
                     QIFDLAERAERDAVLFVTELQRLYPDLAKDEMAIILKEEKSHLKKVLERKKESQPMFG
                     RGM"
     gene            4060..4203
                     /locus_tag="PLD67_02060"
     CDS             4060..4203
                     /locus_tag="PLD67_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21344.1"
                     /translation="MADKNIKKENKKKKKKETKQANVSSVSYEQPVMSQPELVPKKKK
                     KDE"
     gene            4391..5563
                     /locus_tag="PLD67_02065"
     CDS             4391..5563
                     /locus_tag="PLD67_02065"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOT21345.1"
                     /translation="MNILILNCGSSSIKFQIAEMPDYNVIIQGSVERIGYEDAEFKFF
                     LVKQKIIQPVLSHEEGINLIINTLFKSNFGIETKDINAIGHRVVQGGDKQNISVLIDD
                     KVIDYIESIKDLAPLHNPNHLAGIYACRKLLPNVPQVAVFDNGFHMSLPRSTYIYGIP
                     YEYYEKYNIRRYGFHGIAFRSMAEEVDKILNETHKNYKIVNLMLGSGTTANALKYGKS
                     VEVSTGFTPHEGLIQSTRAGDIDAAAILYLMKKENLSIEQADELINKKSGWKGLSGIS
                     NDMREIYQASQSGNERAKLTIDAVCHRFKKYVGAYSAIMEGIDILVFSGGVGQNAPYI
                     REKVCKGLEYMGIELDEYKNQNTAGIISKDSSKVKVIVVNANEEKIIAQDTYEICK"
     gene            5710..6360
                     /locus_tag="PLD67_02070"
     CDS             5710..6360
                     /locus_tag="PLD67_02070"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HOT21346.1"
                     /translation="MNKNCFVSWSGGKDSCLALYKAIEMGYKPVKLFTMFGTEKISSS
                     HRLDENIIKLQADSIGIEYLIGKSDFDEYEKVFVSNLKSFKDQGIDYGIFGDIDLDEH
                     RQWEERVCKSAGMEAILPLWKRDRKEIVKEFISLGFKAKIVVINTSMTDIKFLGKDLS
                     LSLIEEIEKAGADPCGENGEYHTLVYDGPIFKKPVDIKYDYEIIPVGETWAQIKVY"
     gene            6426..6794
                     /locus_tag="PLD67_02075"
     CDS             6426..6794
                     /locus_tag="PLD67_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lin0512 family protein"
                     /protein_id="HOT21347.1"
                     /translation="MVDRFIIEFGMGVDFHGQDVTKAAVKAAKDAISKSCLIGLNEIC
                     GFNQDNVDDKVLIDVTIGVTRPDEVKIDEVVKCFPVGKVTVKAVYGGLKTEGLYFSKF
                     GDKDNSIEVAVASVSVKIKK"
     gene            6806..>7082
                     /locus_tag="PLD67_02080"
     CDS             6806..>7082
                     /locus_tag="PLD67_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012344568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT21348.1"
                     /translation="MKIAIIGGGPGGYVAAIRAAQLNAEVTLIEKEHIGGTCLNKGCI
                     PTKVLLQTSGEFEAVNKSFKDLGIKISGAELDWKQLQKRKTKIIRKLV"
BASE COUNT         2568 a         1053 c         1523 g         1938 t
ORIGIN      
        1 gatgttttca taggctcaaa ggcaatatgg aatatagata caatcgaaaa aatcattgca
       61 accagatctc acccggcttc catcggtttt tcctccctgg tggggtgcaa aaaaattatt
      121 tatccggaag atgatttcgg agcctatgtg gatatcaact caggaagcgt aaggattaag
      181 gcccccgttg cagcgggagt ggtggaatcc gtatctgtaa gcaatccggt aattttgaga
      241 ttagaggacg agtatgaatt cacggccaag gacaggggca ccatagcttt agatggagaa
      301 agggaaattg aattcaaaaa ggatcagaag ttgattttta aaattacaag agagggtcct
      361 tatcacgttg atgttataaa ggctttagaa actgcacaag aaaacaattt tttcattata
      421 aaaaactgat actaaggagg taatatggct gaattcattg taatgccaaa aatggggctt
      481 accatgacaa agggcatgct gacgaattgg ataaagaaag aaggggactt agtaaataaa
      541 ggcgatatcc tgtttgaagt ggaaacggat aaaataacaa acaaagttga agccaagata
      601 tccggaattt taagaaaaat cttggttgac aaggaaaccg tagatgtttt tgtacctgtt
      661 ggaattatag gtagggcgga tgaagatatt tctgaattgt tggagcaggc atcaagagag
      721 cttaaaacgg ataagccgca aaaggaagaa ggtcccaaag aaacaaggga actatcgccg
      781 gctgtacaag ccggagatag agtgaagatt tctccgagag caaagaagat agcaaaggat
      841 ttaaaggtgg attataattt ggtgcaggga acgggaccgg gcggcgccat tacggaagaa
      901 gacataagaa aatttgccga aggtacaaaa gcaaaggtat ctccgacagc ggctatagtt
      961 gccgaacaat tgggagtaga catcttcaat attgaaaaag acaccaggat aatgaaggca
     1021 gatgtaatta aatataaatt ggatgaaagc ttggcaaaat atgcagatcc ccaagaacac
     1081 cgccaatcca tgaccaatat gcgaaagatt attgcaaaga gaatgcttca cagcgttcaa
     1141 acatctccat ccgtatcatt taacataaaa gttgacacaa ccgggatgaa agagctgaga
     1201 gatgaattaa aggatacatt caaggtatct tacacggata ttcttgtaaa aatagtatcc
     1261 gaagtattgc ttgagtttcc tcttttaaac agcacccttg acggagatga aataataacc
     1321 cggaattatg taaacatggg tgttgccgtt gcccttccgg caggactcct ggttcctgtg
     1381 gtaaaattca caaacataaa aggcttaaaa gaaatttcca atgagattaa agacctaagc
     1441 gagagagcaa aaaccaatga tttattgccg gatgaactga ccggcggaac attcaccatc
     1501 agtaatctgg gaatgtatgg aatcgaatcc ttcaccccca tcataaatca gcctgaagtt
     1561 gcaatattgg gagtaaacgc aattcataag gagccggtcg tagtaaatga tgaagttgtt
     1621 attaagccaa tgatgaattt aagccttacg gcagaccaca gggtggttga cggggcggtt
     1681 gcagccgaat tcttagcaaa attaaaaaaa tacattgaaa atccgtgctt gcttttatta
     1741 tagatactgc cttgagatgt ctcaaatccc ggttgaagta tttgcaaatg atgatataat
     1801 ataacaaata atgttttgta cataagcgga caaacaacaa taggaggaaa caatggaatt
     1861 aaaaaattac caggatttga ttgacagaat tcgcaaaact gattcgaaaa aacgtgtagc
     1921 cgttgctgca gcccatgatg agcatacatt ggaagctgtt ttccgagcag ctgatgacaa
     1981 attggttgaa cctgtattaa taggaaataa agcaaaaatc atagaaatat taaaagagct
     2041 taatgtagaa tacgatttaa acagcattat tagtacagat agcgacaagg aatctgcaga
     2101 aaaaaccgtt gaattaataa attcaaacat ggcagacttc attatgaaag gaaagcttca
     2161 gactgcagat ttattgaggg cggtagttga caaagaaaaa ggcttgagaa caggcagggt
     2221 aatgtcccat gttgcatttt tggaaattcc ggcataccat aagctgattg ccgttacaga
     2281 cggcgggatg atgatgtatc caaatgcaga ggaaaagaag cagatactcg aaaatgttgt
     2341 cgatgttttc cgtgccatgg gatacgagtg tcccaaggtt gcagtccttg cagcggttga
     2401 aaccgtgaat ccaaaaatgc ctgaaacagt tgatgcggat attcttaaaa aaatgaatca
     2461 atcgggagaa ataaaagatt gtttagttga aggacccatt tccgtggatt tgactttcaa
     2521 cagggaatca gcagaaatca aaggttttaa aagtcctgtt accggagaag cggatatatt
     2581 gttggttccg agcataacga caggaaatat aatgtgtaaa gcattgatag aaatggcagg
     2641 cgcaactatg gcaggaatga tagtgggagc aaaagtcccg gtagtattgg catcaagagg
     2701 cgcatcatca caggaaaaat atttatcgat agttttgtct gcttcatcag taaaataaat
     2761 attttggaat aaaatacggg gacattcctt taacgcactc tcaaagattt tcctgcgtta
     2821 aaggtgtgtc cccatacttc taatgaatgg gtcttgggca tctcgcattg aaatttgttt
     2881 agtccatctt aactgaatct gtttgtagca gattttgata tatttttaag tgctatttca
     2941 gcctcatcct gcagtcttgg ttcaacagaa atatcaccta atgatacgat tcctactaat
     3001 ctgttatttt ctacgacagg aagtcttcta atctggttct cgctcattat tctcacagca
     3061 tcctcaacgt ccatatcggg agttcctacc acaggatttg cagtcattac tttttttacc
     3121 tgcaaataac catcatcgcc cacagctact gctcttaaag caatgtccct atcggtaatt
     3181 atgccgatta cgctgttttg cgcacatatc ggaattgagc cgacattgta agttttcata
     3241 agctgcgcag ctttttcaac agtatcttct gcacttaaac atgcaacttc tcttgacata
     3301 atgtctctta atttcaaaat atcacctcca aatatatatt tacctttatt ctttgttgta
     3361 ttcagattta tgaataaaaa atgaattttc ggtatttatt gtttaaagca gtccctgatg
     3421 ggtaaaagta tttatatatt aaaaggaggg tattttatat gagtattgta aaatggaacg
     3481 gaagaaattt acttaaatca atagctgaaa atgaaaaaga agcaaccaag ttatacagaa
     3541 tgtttgcaag tgaagcaaga ataggagaga agttttttga actgttggca aaggatgaag
     3601 aaagacacga aaaaatctac aatgcactgc ttgaaaaata ttcagataaa ctggaactgg
     3661 aaatggaaga atccgatgcg gaatacatgg atttattagt tgaaagcaat atcggtttcg
     3721 atgatgaact agttgaaaag gcaaaaaaaa tatttactaa atcacaaata tttgatttgg
     3781 cagagagagc tgagagagat gcagtattat tcgttaccga attgcaaaga ttgtatcccg
     3841 atttggcaaa ggatgaaatg gcaattatct taaaggaaga aaaatctcac ttgaagaaag
     3901 ttctggaaag aaaaaaagaa agccagccaa tgtttggaag aggcatgtaa aatattatct
     3961 tctttaaaaa aataatgcat tattattcca tttgtattat aatagtgtca caaaatcaat
     4021 ttgacacaaa tgaaaaatgc aaataaagga gttaataaaa tggcagataa aaacattaag
     4081 aaagaaaaca agaaaaaaaa gaaaaaagaa acaaaacaag caaatgtatc atcagtatct
     4141 tatgaacaac ctgttatgag tcagcctgaa ttagttccta aaaagaagaa aaaagatgaa
     4201 tagtatctaa aaatgtgtct cggatatacc ggacacattt tctttattgc agtaattgat
     4261 tgtttttttt atatgttgta taattaaata agttttttgt cacaagagag agttcgaata
     4321 atctcaacat attgttttaa cagcaaagga taaagggata tatcatggtc tcggtaaatc
     4381 aggagggaat atgaatattt tgatattaaa ctgcggcagc tcatcaatca aatttcagat
     4441 agcagaaatg cctgattaca atgtaattat acagggcagc gtagaaagaa taggatatga
     4501 agatgcagaa tttaaatttt tcttagtaaa gcaaaaaata attcagcctg tgctaagtca
     4561 cgaagaaggc attaatttaa tcattaatac tcttttcaaa agtaattttg gaattgaaac
     4621 aaaagacatt aatgcaatcg gtcatagagt tgtgcaggga ggagataagc agaatatttc
     4681 ggtccttatt gatgataaag ttatagacta tatagaaagt ataaaggact tggcccctct
     4741 tcataaccct aaccatttgg caggaattta tgcatgcagg aagcttcttc ccaatgtacc
     4801 gcaggtggct gtatttgata acggatttca tatgtcgctt ccaaggtcta catatatcta
     4861 tggtattcct tatgaatatt atgaaaaata taatatcaga agatacggct ttcatggtat
     4921 tgcattcaga agcatggcgg aagaagttga taaaatatta aatgagactc ataagaatta
     4981 taaaatagta aatttaatgc ttggaagcgg aacaacggct aatgctttaa aatacggcaa
     5041 atcagttgaa gtgagcacag gtttcactcc tcacgaagga ctgatacagt ctacaagagc
     5101 cggagatatt gatgctgctg ccattttata tttaatgaaa aaagaaaatt tatctattga
     5161 acaagcggat gaattaataa acaaaaaaag cggatggaaa ggtctttccg gtatcagtaa
     5221 cgatatgcgt gaaatctacc aagcatcaca aagcggaaac gaaagagcga aactcacaat
     5281 tgatgctgta tgccacaggt ttaaaaagta tgtgggtgct tacagtgcca taatggaagg
     5341 tattgatatt cttgtgtttt ccggtggagt aggtcagaat gccccttata taagagaaaa
     5401 agtatgcaag ggattggaat acatgggaat tgagctggac gagtataaga atcaaaacac
     5461 tgcaggtata atcagcaaag attcatcaaa agtaaaagtt attgtagtta atgcaaatga
     5521 agaaaaaata attgcccaag atacatatga aatatgcaag taagtctgag cgataggaaa
     5581 ggattatcca taaacaatcg gaatactcat tgctatagta ttttatgcat tattgggtat
     5641 aattaataaa aatatatact aatgctgtta taagctgtca actaataact aagtatagag
     5701 aggtagaatt tgaataaaaa ttgttttgtt tcatggagcg ggggcaagga ttcctgtctt
     5761 gcattatata aggcaataga gatgggatat aagcccgtca agctttttac catgtttggc
     5821 acggaaaaaa tcagttcctc tcacagattg gatgagaata ttataaaatt acaggcggat
     5881 tcaataggaa ttgaatacct gatagggaaa tcagactttg atgaatatga gaaggtattt
     5941 gtttcaaatc taaaaagttt taaagaccag ggaattgact atggaatatt cggagacata
     6001 gatttagatg aacatagaca gtgggaagaa agagtatgca aatcagcagg tatggaagct
     6061 atcctgcccc tttggaagag ggatagaaaa gaaatagtaa aagaatttat ttcgcttgga
     6121 tttaaggcta aaatagttgt tatcaacacc agtatgacag atataaagtt tttgggaaaa
     6181 gatttaagtc tgtctttgat agaagaaatt gaaaaggcag gagctgaccc atgcggcgaa
     6241 aatggagaat atcataccct tgtgtatgat ggtcccattt ttaaaaaacc ggtggatata
     6301 aaatatgatt atgaaattat tcccgtagga gaaacatggg cccagattaa agtatattaa
     6361 agggatacaa ttattgttga ataaacatta ttttataaac tatactaata atataaaggg
     6421 gtgatatcgt ggacagattt attattgaat ttggtatggg agtggatttc catgggcaag
     6481 atgtaacgaa agctgcagta aaagcagcaa aagatgccat atccaaaagt tgccttatag
     6541 gtcttaatga aatatgcgga tttaaccagg ataatgttga tgacaaggtg ctgattgatg
     6601 ttacgattgg agttactcgt cctgacgagg tgaagattga cgaggttgta aagtgttttc
     6661 ctgtaggaaa ggtaaccgta aaagcggttt atggcggttt aaaaaccgaa ggtctttatt
     6721 tttcaaaatt cggagataag gataattcaa ttgaagttgc agttgcaagt gtatctgtta
     6781 aaattaaaaa gtaaggggag gaataatgaa aatagcaata ataggcggag gccccggcgg
     6841 ctatgtagca gctattaggg cggctcagct taatgcggaa gtaacgctaa ttgaaaaaga
     6901 gcatatcggg ggtacatgcc tcaataaagg atgtatacct actaaggttc tgcttcaaac
     6961 ttcaggagag tttgaagcag tcaataaaag ttttaaggac ttgggcataa aaatatccgg
     7021 agcagaatta gattggaaac agttacaaaa aagaaaaact aaaataataa gaaaattagt
     7081 ag
//
LOCUS       DAOSOW010000045         7065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_10686_length_7065_cov_3.609700, whole genome shotgun sequence.
ACCESSION   DAOSOW010000045 DAOSOW010000000
VERSION     DAOSOW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10686_length_7065_cov_3.609700"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="PLD67_02085"
     CDS             <1..722
                     /locus_tag="PLD67_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOT21349.1"
                     /translation="LMSHIGSFVPAKKAEICIIDKIFTRVGASDDLSQGQSTFMVEMS
                     EVSNILKNATSDSLLILDEIGRGTSTYDGLSIAWSVVEYITKKLKAKTLFATHYHELS
                     ELESKLKSVKNYKILIKEEHDEITFLRKITEGSIDRSYGIQVANLANLPEEVIERAKE
                     ILIQLEESDINKPFARKKNRVNDNFQVSMFQANPAENEINKKYKELAENIKNIDIENI
                     TPMQSFKLLNDIIEKTKRIDT"
     gene            740..2581
                     /gene="mutL"
                     /locus_tag="PLD67_02090"
     CDS             740..2581
                     /gene="mutL"
                     /locus_tag="PLD67_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOT21350.1"
                     /translation="MAIINLLDNETINKIAAGEVIENPKSIVKELVENSIDAGADEIT
                     IEIKNGGKSFIRVTDNGKGIEKDQVENAFKRHCTSKISSSDDLNSLFTLGFRGEALAS
                     IAAVSKVEVITRTNDDIYGIKMTIEGGKIISKEDIGCPVGTTITVTDLFYNVPARKKF
                     LKSDISESSNISEIVVSLALSKENISFKYINNNNVAFRTPKTNNLLNTISSLYEKDLY
                     NSLLKIDYKSDIISITGYTTNLNYYRGNRKQQIVFVNGRVIKHRRMSYFIEEAYNTLL
                     PKNKYPACFIKIEIAPALVDVNVHPAKTEVRFQNEEMVLNEIKNAVYSALHSKSIMKE
                     VKGETLLHDSKDINNLRIFDRSSGKSVILGGLENKFTEETEETKNIRITEVEVSNKDI
                     AVMEPLKPYDKFEFKQQTFIKAENDEDKLPELYVIGIVLDTYIICENPEKTCIFMIDQ
                     HAAHERINYEKFLNQYNSREILIQEIVPEVINLSYDDYETAMSNIEIFEYLGISVENF
                     GNNAILINNIPTIFKDSNLKDVFYAILDNLKDITKSNLDFVMEKIIKEACVKSVKSGD
                     RLHMSEVRALLNDLRMTDNPYTCPHGRPVIIKMTRYEIEKMFERIQS"
     gene            2591..3541
                     /gene="miaA"
                     /locus_tag="PLD67_02095"
     CDS             2591..3541
                     /gene="miaA"
                     /locus_tag="PLD67_02095"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010282246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOT21351.1"
                     /translation="MNKIIVIVGPTAVGKTYISVELAKKLNTEIISADSMQIYKGMNI
                     GTAKITEEEKQGIIHHMIDIINPDEEYSVSEFKYDAEKIIDRLLSENRIPIIVGGSGL
                     YVNSLIYDLDFGNTKSNKKLREYYTYYYKEHGEDALYDKLLKIDPVAAEKIHKNNIKR
                     IIRALEVYDITGVKFSELNTDIRKKSNKYDCILIGLSMERKVLYERINQRVDEMLSKG
                     LVDEVSSLIKKGYGKNLVSMRGIGYKEIIEYLEGNTDYEEAVNTLKQNTRRFAKRQYT
                     WFLKDENVKWFSMDNPSEIDKTLDSIVEYISTRIDSISNQ"
     gene            3633..3869
                     /gene="hfq"
                     /locus_tag="PLD67_02100"
     CDS             3633..3869
                     /gene="hfq"
                     /locus_tag="PLD67_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOT21352.1"
                     /translation="MNIINLQDTFLNKVRKEKITITLYLNNGFKLTGTVLGFDNYVIF
                     IETDKGQQLVYKHAITSILPFKQVRLFFDEPEEQ"
     gene            3869..5167
                     /locus_tag="PLD67_02105"
     CDS             3869..5167
                     /locus_tag="PLD67_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HOT21353.1"
                     /translation="MLTENKLCKQFGISEAVYNYVTKIEKEIKKNHFEKIDKIRELNQ
                     YKVIHAMQENKLDYTNFYWNTGYGYGDVGREKVEKIYASVFKTEDALVRPSIASGTHA
                     LYLTLSGILQHGDEVISISGRPYDTLLTVLGVQGKEPNNLSEAGIIYKEIPLYKNDID
                     WESAIKEVSMKTKLLMIQRSTGYSFRPALTIEKIKNAIERIRQVYPNIYIMVDNCYGE
                     FVDEMEPSDVGADVVVGSLIKNPGGGVALSGGYVVGKKVLIDRIANRLTAPGVGKEIG
                     LTFGTTRNTLQGLFFAPHIVSESLKGALLFGGVYSSLGFEITPTVEEDRSDIIQAIKF
                     NDKNMVIKICEAVQAASPVDGYVSPIPWDMPGYTNQVIMASGAFVSGSSIELSADAPM
                     RPPYIAYIQGGLFYDHVKLGVMLSLEKMMEFNEIKNKIFN"
     gene            complement(5238..5861)
                     /gene="lexA"
                     /locus_tag="PLD67_02110"
     CDS             complement(5238..5861)
                     /gene="lexA"
                     /locus_tag="PLD67_02110"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOT21354.1"
                     /translation="MSYEGLSDKQVKILQYIKDELTLRGYPPSIREICKAVGLNSTSS
                     VHAHLNTLEEKGYIKKGTNKRRALELIDLDDICCDMPKKEIINLPIIGTVTAGSPILA
                     VENIDDTLPISIDFVGNKESYVLKVKGDSMIEAGILHGDYVIVNSQRNAKNGDIVVAL
                     IGDEATVKTFYKEKDHIRLQPQNSSMDPILIKEPYILGVVKAVVRKY"
     gene            6091..6393
                     /locus_tag="PLD67_02115"
     CDS             6091..6393
                     /locus_tag="PLD67_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT21355.1"
                     /translation="MIILNKYKVSDAKKFKRFMFLSILLISIITITSITTIKAYSKDI
                     PQYDYVRVQHGDTLWSIAKDYAGSKDIRQVVYEISELNKIHNNLIHIGDIIKIPLN"
     gene            complement(6396..>7065)
                     /locus_tag="PLD67_02120"
     CDS             complement(6396..>7065)
                     /locus_tag="PLD67_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT21356.1"
                     /translation="RSYFKYLHTIVKLIPENPALELETPKNKSRLPVALTLDESRSLL
                     SSISGSNEKRDYAIIMLFLNCGLRLSELISIDIDKIKGDTLTVIGKGNKERTIYLNDV
                     CVKAVEDYLEVRQEPKKGHEKALFISNRKTRFSQKGIQHMLDKYLTEAGLSGKGYSPH
                     KLRHTAATLMYQYGHVDIRALQEILGHESISTTQIYTHINKELLRDAVKQNPLNDMND
                     DESL"
BASE COUNT         2567 a         1082 c         1323 g         2093 t
ORIGIN      
        1 cactaatgtc tcatataggc agttttgttc ccgctaaaaa ggcggaaatc tgcataattg
       61 ataaaatatt tactcgtgta ggagcatcgg atgacctctc acagggacaa agtaccttca
      121 tggtagaaat gagcgaggtt tcaaatattt tgaagaatgc tacttctgac agccttttga
      181 tattggatga aatcggaaga ggtacaagca cctatgacgg gttaagcatc gcttggagtg
      241 tagtagaata tattacgaag aagctcaagg caaaaacatt gtttgcaact cattatcacg
      301 aattgtccga gcttgagagc aagcttaaga gcgttaaaaa ctataaaata ttaattaaag
      361 aagaacatga tgaaataaca ttcttaagaa aaataactga aggcagcatt gacagaagtt
      421 atggaataca agttgcaaat ttggcaaatc ttcctgaaga agtaattgaa agagcaaaag
      481 aaatattaat tcagcttgaa gaaagcgata taaacaagcc ttttgctagg aaaaaaaaca
      541 gggtgaacga taatttccaa gtttccatgt tccaggctaa ccctgcagag aatgaaataa
      601 acaagaaata caaagagctg gcagaaaata ttaagaatat agatattgaa aatataacac
      661 ctatgcaatc atttaaattg cttaatgata taatagaaaa aactaaacgg atagatacat
      721 aacgaaagga atgtcttaca tggctataat aaatttattg gacaatgaaa ccattaataa
      781 aatagctgcc ggagaggtta ttgaaaaccc aaagtctata gtaaaggaat tagtcgaaaa
      841 ttcaattgat gccggtgccg atgaaataac gattgaaatt aaaaacggcg gcaaaagctt
      901 tatacgagta accgataacg ggaagggaat tgaaaaagac caggttgaaa atgcctttaa
      961 aaggcattgt accagcaaga tttcttcttc agacgatttg aattcgcttt ttactctggg
     1021 atttagaggt gaagccttgg caagcattgc tgccgtgtcc aaggttgaag ttataacaag
     1081 gacaaatgat gatatctatg gcattaaaat gactattgaa ggcggaaaaa tcatttctaa
     1141 ggaagatatc ggctgtcctg tcggaacaac aataaccgta acagaccttt tttacaatgt
     1201 gcctgccagg aagaaattct taaaaagcga tatttccgaa agctcaaata tcagtgaaat
     1261 tgttgtgtcc ctggctttaa gcaaggagaa tatttcattt aagtatataa acaataacaa
     1321 tgttgccttc agaactccaa agacaaataa tctgctaaat actatttcaa gtctttatga
     1381 aaaagatctt tacaattcgc ttttaaagat agattataaa agcgatatta taagtattac
     1441 gggatataca acaaatttaa actattacag gggtaataga aagcagcaga ttgtttttgt
     1501 taacggaagg gttataaagc atagaaggat gagttatttc attgaagaag catataacac
     1561 tcttcttccc aaaaataaat atccggcatg ctttattaaa attgaaatag ctcctgcatt
     1621 ggttgatgta aatgttcatc ctgcaaagac ggaagtgcgc tttcaaaatg aagaaatggt
     1681 attaaatgaa ataaaaaatg ctgtttattc tgccttgcac tccaagagta ttatgaagga
     1741 agtaaaaggg gaaaccctct tacatgattc aaaagatata aataacttaa gaatatttga
     1801 tagaagttca ggtaaatctg tcatcttagg cggtttagaa aacaaattca cggaagaaac
     1861 agaagaaaca aagaatatta ggattacgga agttgaagtt tcaaataaag atattgccgt
     1921 aatggagccg cttaaacctt atgataaatt tgaatttaag caacaaacat tcataaaggc
     1981 agaaaatgat gaagataagc tgcctgagtt atacgttata ggcatagtgc tggatacata
     2041 tataatttgc gaaaacccgg aaaaaacatg tatattcatg atagaccagc atgcggcaca
     2101 tgaaagaata aactacgaga aatttttaaa tcaatataat tcaagagaaa tcttaataca
     2161 ggaaatagta ccggaagtga ttaatttatc ctatgatgat tacgagacag caatgtcgaa
     2221 tatagaaata tttgaatatt tgggaatttc cgttgaaaac tttggaaata atgctatact
     2281 gataaataac ataccaacta tatttaagga ttcaaatttg aaggatgtat tttatgcaat
     2341 cctggataac ctaaaagata taactaaatc aaatttagat tttgttatgg aaaaaataat
     2401 aaaagaggca tgcgtcaagt ccgtgaagtc cggagacagg cttcatatgt ctgaagtaag
     2461 agcattgtta aatgacctta gaatgacaga taacccttac acttgtcctc acggaaggcc
     2521 tgtaattatt aaaatgacta ggtacgaaat tgaaaaaatg tttgaaagaa ttcaaagtta
     2581 ggaaagcaaa atgaataaaa taattgtaat tgtaggtcct acggctgtcg gcaaaacata
     2641 tatatccgta gagcttgcaa aaaagttaaa tactgagatt atatcagccg attcaatgca
     2701 gatatataaa gggatgaata ttggaactgc aaaaatcacc gaggaagaaa aacaaggtat
     2761 cattcatcat atgatagata ttataaatcc tgatgaagag tattcggtgt cagaatttaa
     2821 atatgatgcc gaaaaaatta ttgacaggct tttgagtgaa aacagaattc ccataattgt
     2881 aggggggagc ggcctgtatg taaattcttt gatatatgat ttagactttg gcaataccaa
     2941 gtccaataaa aaattaagag aatactacac ttattattat aaggaacatg gggaggatgc
     3001 tctgtatgac aaattactga aaattgaccc tgttgctgct gaaaaaattc ataaaaataa
     3061 tataaagaga attattagag ccctggaagt gtatgatatt acaggagtta agttttcaga
     3121 gctaaacacg gacataagaa agaaaagcaa taaatatgac tgtatattga ttggtctttc
     3181 aatggaaaga aaagtccttt atgaaagaat taatcaaaga gttgatgaaa tgctttctaa
     3241 gggtcttgtt gatgaggtta gctcacttat taaaaaggga tacggtaaga atttagtatc
     3301 catgagggga attggatata aagaaattat tgaatatctt gaaggtaata ctgattatga
     3361 ggaagcagtc aatactttaa aacaaaatac aagaagattt gcaaaaaggc agtatacttg
     3421 gttcctgaaa gatgaaaacg tcaaatggtt cagcatggat aacccaagcg aaattgacaa
     3481 aactttggat agtatagttg aatatataag cacgagaatt gatagtatta gtaaccaatg
     3541 agataaataa taataaaaat atacgctttc gcttaatttt attattatag taatgaagta
     3601 ataacatata tgcaaattta taggagaagg gaatgaatat tattaattta caagatactt
     3661 ttttaaacaa agtaagaaaa gaaaaaataa caataacgct ttatttaaac aacggtttca
     3721 agctgacggg aactgtctta ggttttgata attatgtaat ttttatcgaa acagataaag
     3781 gtcagcaact ggtttacaag catgcaataa catcaatttt gccgtttaaa caggtacgac
     3841 tgttttttga cgagccagag gagcaataat gttaacagaa aataaattat gtaaacagtt
     3901 tggcataagc gaagctgttt ataattatgt aacaaaaatt gaaaaagaaa ttaagaagaa
     3961 tcattttgaa aaaatagata aaattagaga gcttaatcaa tataaagtta tccatgcaat
     4021 gcaggagaac aagcttgatt atacaaactt ttactggaat acaggctacg gttatggaga
     4081 tgtcggaaga gaaaaggttg aaaaaatata tgcatccgtg tttaagaccg aagatgcatt
     4141 ggtaagacct tccatagctt caggaaccca tgccctgtac ttgacattgt caggcatatt
     4201 gcagcacggt gatgaagtaa tttccatatc cggacgacca tacgatactt tgcttacagt
     4261 attgggcgtg caaggaaagg aacctaataa tctcagtgaa gcaggaataa tttataagga
     4321 aattcctctt tataaaaatg atatcgactg ggaaagtgca ataaaagaag tttcaatgaa
     4381 aacaaagctt ttaatgattc agcgctcaac aggctacagc ttccgccctg cgcttacaat
     4441 cgaaaaaata aaaaatgcaa tagaaagaat tcgccaagta tatccaaata tatatattat
     4501 ggtagataat tgctacggag aatttgtaga cgaaatggag ccttcggatg taggggcaga
     4561 tgtagttgta ggctcgctta taaaaaatcc cggaggggga gttgcattat cgggaggcta
     4621 tgtggttgga aaaaaagtat taattgacag aatagccaac cgtcttacag ctccgggagt
     4681 cggaaaagag ataggactta cattcggaac cacaagaaac acgcttcaag gtttgttttt
     4741 cgcaccacac attgtgagcg aatcattaaa aggcgcactc ttgttcggcg gggtttactc
     4801 ttctctcgga tttgaaataa ctcctacagt agaagaggac agaagcgata ttatacaggc
     4861 aattaaattt aacgacaaaa acatggtaat taaaatttgc gaggctgttc aagctgcatc
     4921 tccggtggac ggttatgtgt ctccaatacc atgggatatg cccggctata caaatcaggt
     4981 aataatggct tcgggagcat ttgtttccgg ctcatccata gagcttagcg cagatgcacc
     5041 aatgagacct ccttatatag catatattca gggcggcttg ttttacgacc atgtcaagtt
     5101 gggagtaatg ctatcacttg aaaagatgat ggaatttaat gagataaaaa ataaaatatt
     5161 caattaaaaa tatggggaca ttccttatac tacaaagact atctttatag taaaggcgtg
     5221 tccccatacc tatgacatta atattttctg acaacagctt taacaacacc caatatatag
     5281 ggctccttta ttaaaatcgg gtccatggaa ctgttttgag gctgcaatcg aatgtggtct
     5341 ttttctttat agaaggtttt aactgttgct tcatctccga ttaatgcaac aactatatct
     5401 ccatttttag cattgcgctg ggaattaact attacataat caccgtgcaa aattcctgcc
     5461 tcaatcatac tgtctccctt tacttttaaa acatatgatt ccttattacc tacaaaatca
     5521 atcgaaatag gcagggtgtc atcaatgttt tcaacagcta atataggaga gccggcagtt
     5581 acggttccta taatcggtaa gtttataatt tctttttttg gcatgtcgca gcatatatca
     5641 tcaagatcaa ttaattccaa agcccttctt ttgtttgttc ctttttttat gtatcctttt
     5701 tcttctagtg tatttaaatg ggcatgaacg gaagaagtag agtttaaacc tacagctttg
     5761 caaatttcac gaatagacgg gggataaccc cttaaagtta actcatcttt tatatattgc
     5821 aatattttaa cttgtttatc gcttaagcct tcataactca taattccacc tctttataaa
     5881 attattttag actcccggga gcactttttc gcttcgtccg gatagaacct atctgaagca
     5941 tcgatttaag ctgtctgatt gagtactttt ctatatttta acatagtttg acaagtaaag
     6001 caaacatttg tttgaaaaaa tgtaaaaaat tgttgacatc gaactaatgt tctgttagaa
     6061 tgaaaacaga aaatatgttc gaggtgcctt atgataatat taaataaata taaagtaagt
     6121 gatgcgaaaa aattcaaaag atttatgttc ctgtccattt tgcttataag tattattact
     6181 attacctcaa ttactacaat aaaagcatat tcaaaagata tacctcaata tgattacgta
     6241 agagttcaac acggagatac cttatggtca atagcaaaag attatgccgg cagtaaagat
     6301 ataagacaag tagtatacga aatatccgag cttaacaaga tacataataa cctgattcat
     6361 atcggcgaca taattaaaat tcccctaaat taaacttaaa ggctctcatc atcattcata
     6421 tcatttaagg gattttgttt taccgcatca cggagcaatt ccttgtttat atgagtgtaa
     6481 atttgcgttg ttgaaatact ttcgtgtccc agtatttctt gcagcgctct aatatctaca
     6541 tggccatatt gatacataag ggttgctgcc gtatgtcgaa gcttgtgagg ggagtatcct
     6601 tttcctgata aacctgcttc agttaagtat ttatccaaca tatgctgaat gcctttttga
     6661 gaaaaacggg tttttcgatt acttataaaa agagcttttt catgaccttt tttcggctct
     6721 tgtcttacct ccaagtaatc ttctacagct tttacacata catcattcag gtaaattgtt
     6781 ctttccttgt tgccttttcc gattacagtc aaggtgtctc ccttaatttt atcgatatcg
     6841 atgcttataa gctctgataa tcgcaggccg caattcaaaa ataacataat aatggcatag
     6901 tcgcgttttt cattggagcc tgatattgat gataataaac ttcttgattc atccagcgtt
     6961 aatgctaccg gcagacggct tttgtttttt ggagtttcta attcaagagc cggattttca
     7021 ggtataagct ttactatggt atgtaaatat ttaaaatatg accta
//
LOCUS       DAOSOW010000046         6998 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_10816_length_6998_cov_3.763215, whole genome shotgun sequence.
ACCESSION   DAOSOW010000046 DAOSOW010000000
VERSION     DAOSOW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6998
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10816_length_6998_cov_3.763215"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1395
                     /locus_tag="PLD67_02125"
     CDS             169..1395
                     /locus_tag="PLD67_02125"
                     /inference="COORDINATES: protein motif:HMM:NF016083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF401 family protein"
                     /protein_id="HOT21357.1"
                     /translation="MTQLFIVFIVFLMIPLLSRFKIQLSYILLIACCILALLSGMGAE
                     AALQSVKNTFTELSSVNTILSVMMVSVLGGLMKHYKILDKIVESIYKIIRNKKIILMV
                     LPALMGVLIVPGGALLSAPFIDEIGEEMKIPAPRRAAINLVFRHIAMFILPFSTGLLV
                     ISAALPEINISKLIFLNLFYVIPTTFVGYFLFIKDIKYENTQKVKLSGRNFLTLLLYT
                     SPIYICVIINSVTGLPFFVALIFSVLAVYLLCDKKQFFSILIKSVNYHTVLTVAAILI
                     MKELILNMEELIILFKSMFNAESSMLSILLVFLVSSVFFGIITGNQTATIAIIVPMIS
                     QLGISTDMIYIYTYFSFACSYAGYYFSPIHLCQAFTIQYMNVTTMELYKEYRLYVIVS
                     FIVPFVSVYLMKFLFV"
     gene            complement(1384..2505)
                     /gene="dprA"
                     /locus_tag="PLD67_02130"
     CDS             complement(1384..2505)
                     /gene="dprA"
                     /locus_tag="PLD67_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOT21358.1"
                     /translation="MDNLNINQKTLLWLSSGNVSNYNISKLLDYFGSVQNLWENFENE
                     QLNLNFLKVETLNKLSKIKIDFEERILEKLNSEKAQIVTIFDNDYPSKLKQIEGAPYL
                     LYYKGSLEDINNISIAVIGSRKATSYGKWAAEKLTKELSDIGVNIVSGLAVGIDTIAH
                     KTALKYNAKTYGIIGCGINIVYPKNNRELFKQIAENKGAVITEYTFDIQPVAFNFHDR
                     NRIISGLSEGVLVIEAQEKSGTLITAGHAANQGREIFAVPGNIDSLYSKGTNALIRDG
                     AKITVSIDDIAEEILELKERVSLKKTFIDLGTLTDDELKIIDCLKSGKNTIYVLEQET
                     KMETGELLGNLTFLEMKGAIKVSADKISLNLSFPSISNK"
     gene            complement(2507..4030)
                     /locus_tag="PLD67_02135"
     CDS             complement(2507..4030)
                     /locus_tag="PLD67_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOT21359.1"
                     /translation="MLSKIKTCVLYGLEGYAVDVETDISGGLPNFNIVGLPDISIKES
                     KERVRSAIKNSRFDFPVSRITVNLAPANLKKEGSQIDLPIAVGILTASKIIKPVDEKI
                     CIIGELSLDGRITAIDGALPMVISMRNKSFNKAIVPEDNKEECGAIEGIEIIPVDSIT
                     QLVDYLNGNLYIEPYKSKYNFQDKPDYYNEDFSEIKGQPAMKRAVEIAAAGMHNILLL
                     GNPGSGKTMIARRIPTILPDLTFEESLEVTKIYSISGLLDNKGLITKRPFRSPHHTSS
                     RTSIAGGGTKPRPGEVSLSHYGVLFLDEIPEFPKSVIEVLRQPMEDGKISISRASGSL
                     TFPAKFMMVASMNPCPCGYLGDPTHECSCSQGQIDKYLGKISGPLLNRIDIQIEVLPV
                     KYDDLKDKRAEESSAVIKKRIVKARNIQRERYRNIGILTNSELSTKGISKFCKVNKES
                     EILLKNAFETLGLSVRAYNKILKVARTIADLENYENIETKHIAEAIRYRSLDRKYWR"
     gene            complement(4174..4671)
                     /locus_tag="PLD67_02140"
     CDS             complement(4174..4671)
                     /locus_tag="PLD67_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOT21360.1"
                     /translation="MYKEELAVISQAVLNEMEGYEFYKLAGEQAKTQGTKEAFMSLAN
                     DELKHAEYLQRLWKILSAGGEVKIEDILASGIEIPSPEIFRWDKVDKAYATLEMSIFR
                     IGMQMEKDSMDFYKEAKEKAKSESGKELFDLLIRWEKVHLDQFTNQYNVLKEEWWAEQ
                     RYFPF"
     gene            complement(4733..5365)
                     /locus_tag="PLD67_02145"
     CDS             complement(4733..5365)
                     /locus_tag="PLD67_02145"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOT21361.1"
                     /translation="MKSLSILIIILIISIVGCSLDKEIDKEIKEEALEIKEDSISDDK
                     QIIENSEEIHEENQQIPEEEEPLPELSAKEPLYDEESENDMTSAPADEPLAELEQTVP
                     TEEPLIEPEQTVPFEEAEEIISQPEEYIGEDRGIGGPIKVLVKMKDDKITDIEVVYHN
                     ETKGVGTNAIDTIISSIIENQTTDVDTVSGATVTSKALLNAVKNAIDKKE"
     gene            complement(5373..5993)
                     /locus_tag="PLD67_02150"
     CDS             complement(5373..5993)
                     /locus_tag="PLD67_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HOT21362.1"
                     /translation="MHKEIKLKSKRNNVFRIVEDEGTYILKKFENHENYIREKEVLSI
                     LKKAGVNVPSIIKAEENYLYLEDLGEVNFLEWYEEQEKNNALNISMVYELCSWFKDFY
                     SAVFEFYKKQFILYDVNFKNFIICDNKVYGIDFEQVKPGHIEEDAGRLVAFGLTYNPS
                     MTEWKMDFRNILINILSNELNIEKEKIISEENKELAAIKKRRGAFS"
     gene            complement(5978..6724)
                     /locus_tag="PLD67_02155"
     CDS             complement(5978..6724)
                     /locus_tag="PLD67_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HOT21363.1"
                     /translation="MKVFSVIGLTKSGKTTTIENIIKELRKRNYTVGSVKEIHFEKFK
                     MDVDGTNTHRHKMAGSQLVTARGAYETDVLFQRKLSMNEILCFYNHDFVILEGVRDTC
                     APKIVTAHDVEGIIERLDETTFAISGQISNSLTEYEGIPVINSLTEIEKLVDLIEKKV
                     FDRLPDMKDECCKKCGYTCTQLCSKILKCEAERRDCVLTENSVTLKINGQEIAMNPFV
                     QNILKNTVEAIAKELYGYTESGDIEVCIKR"
     gene            complement(6721..>6998)
                     /locus_tag="PLD67_02160"
     CDS             complement(6721..>6998)
                     /locus_tag="PLD67_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOT21364.1"
                     /translation="IITAIQLRGIDLKKVKLSKKFQVYKYILTPITVNSIMKAKELAA
                     AMEMRGFRAYSERTSYLVLKMQKFDYILLGLSISAALLFIIITRGAL"
BASE COUNT         1975 a         1358 c         1009 g         2656 t
ORIGIN      
        1 atgctatcca ccatcttttt tactaaaaaa gttgcactta ctttgtcaat tcaccttttt
       61 gattgttaag atttattatc atgcggttag gaatgctcat gtctaataat atattgattt
      121 attctcaata gttttatata attatgttaa tttgcacgga ggggacgtat gactcaatta
      181 tttatcgtat tcattgtttt cttaatgata cctttattaa gtagatttaa aattcagtta
      241 agttatattc ttttgatagc atgctgtatt ttagctttgc tgtccggaat gggagcagaa
      301 gctgcattgc agtcggtaaa aaatactttt acggaacttt catcagtaaa cacaatacta
      361 tcagttatga tggtaagcgt tttaggcggc ttaatgaagc attataaaat tcttgataaa
      421 atagtggaat ctatttataa gataatacga aacaaaaaaa ttatattgat ggttcttcct
      481 gctttaatgg gtgtattaat agttcccggt ggtgcccttt tatccgcacc tttcatagat
      541 gaaatcggcg aagaaatgaa aattcctgct cccagacgag cagctatcaa tttggtattc
      601 agacatattg caatgtttat actgcccttc tcaaccggac tattggttat ttccgctgct
      661 cttccggaaa taaatatatc aaaacttatt tttttaaacc tgttttacgt tataccaaca
      721 acatttgttg gttattttct gtttattaaa gatattaaat acgaaaatac acaaaaagta
      781 aaattatcag gaagaaattt tttgactctt ttactttata catctccaat atatatttgt
      841 gtaattatta attccgtaac aggtcttccc ttttttgttg ctctgatttt cagtgtatta
      901 gctgtctatt tattatgtga taaaaaacaa ttcttctcta tattgattaa atctgtaaat
      961 taccatactg tattaacggt tgcagcgatt ctaatcatga aagaacttat tttaaatatg
     1021 gaagaactta taatattatt caaaagtatg tttaatgcag aaagcagcat gttgtcgatt
     1081 ttattggtat tcttagtatc gtccgtattt tttggaatta taacgggtaa tcaaacagct
     1141 accattgcaa ttattgtccc gatgatttct cagcttggta tatctactga tatgatttac
     1201 atctatacat atttttcttt tgcatgctca tatgcaggtt attatttctc accgattcac
     1261 ttgtgtcagg ccttcacaat tcaatatatg aatgttacaa caatggagct ctataaggaa
     1321 tacaggctgt atgtaattgt ttctttcata gtgcccttcg tttcagtata tttgatgaaa
     1381 tttttatttg tttgatatcg aagggaaact taaattcaga gaaattttat cggcactcac
     1441 ctttattgca cctttcattt ccaagaatgt taaattgcca agcaattccc cggtttccat
     1501 ttttgtttcc tgctctaata catatattgt atttttacct gacttaagac agtctataat
     1561 ttttagttcg tcatcggtta atgtgcctaa atcaataaat gtttttttaa gacttactct
     1621 ttccttcaat tctaaaattt cttccgcaat atcatcgatt gatactgtta ttttagctcc
     1681 atctctgata agagcattgg tgcctttgct gtacaggctg tctatatttc cgggaaccgc
     1741 aaatatttct cttccctgat tagctgcatg acccgctgta atcaatgtac cgcttttttc
     1801 ctgagcttca atgaccaata ctccctcgga aagtccgctg ataattctat tcctgtcatg
     1861 aaaattaaaa gcaaccggtt gtatatcaaa agtatattcg gtaattacag ctcccttatt
     1921 ttcggctatt tgtttaaaaa gctccctgtt attcttcggg tatacaatat ttatgccaca
     1981 accgattata ccatatgttt tggcattgta tttcaaagct gttttatgag caattgtatc
     2041 aattccgact gccagaccgc ttactatgtt tacaccaata tccgacaatt cctttgtgag
     2101 tttctctgca gcccattttc catagcttgt agcttttctg gagcctatga ctgcaattga
     2161 tatattgtta atatcttcta aactgccttt gtagtataac aaataaggag caccttcaat
     2221 ttgtttgagt ttagaaggat aatcattatc aaaaatagtt actatttgag ccttttcact
     2281 atttaacttc tccaatattc tttcttcaaa atcaatttta attttggaaa gcttatttaa
     2341 agtttcaact ttaagaaaat tcaaatttaa ttgctcgttt tcaaagtttt cccataaatt
     2401 ttgtaccgat ccgaaataat cgaggagctt tgaaatattg taatttgaca cgttgccgga
     2461 tgaaagccat aataatgttt tttgattaat atttaaatta tccattttac ctccagtatt
     2521 tgcggtctaa actcctgtac cgaattgctt ccgctatatg ctttgtttca atattttcat
     2581 agttttccaa gtcagcaatt gtccttgcaa cctttagaat tttattgtat gctcttacac
     2641 ttaagcctaa tgtttcgaat gcattcttaa gcaaaatttc agattcctta ttaactttgc
     2701 aaaacttgga tattcctttg gttgaaagct cagaattagt caaaatccca atatttctgt
     2761 atctttctct ctgaatattt ctcgccttta ctattctctt tttaattacc gccgaacttt
     2821 cttcagccct cttatctttt aaatcatcat atttaacggg cagcacttca atttgaatat
     2881 caatacggtt caacaaggga cctgaaattt ttcctaaata tttatcgatt tgtccttggc
     2941 tgcaggagca ctcatgtgta gggtcgccta aatacccgca cggacatggg ttcatggaag
     3001 caaccatcat gaactttgca ggaaatgtaa gggaaccgct tgctcttgaa attgaaatct
     3061 ttccgtcttc cataggctga cgcagaactt caataacact ttttggaaac tccggaattt
     3121 catccaaaaa taaaacacca tagtgagaca gggatacttc tcccggcctc ggctttgtac
     3181 cgcctcctgc tatggatgta cgtgaagaag tatgatgagg tgagcgaaag ggtctttttg
     3241 ttattaaacc tttattgtcc aacaatcctg atatgctgta gattttggtt acttccaaag
     3301 attcttcaaa tgtcagatcc ggtaaaatgg taggaatcct tctggcaatc attgttttgc
     3361 cggagccggg gttgcctaaa aggagtatat tgtgcatccc ggctgcagct atttcaactg
     3421 cccttttcat ggcaggctgt ccttttattt cagaaaaatc ttcattgtaa taatccggtt
     3481 tatcttgaaa attgtatttt gatttatatg gctcaatata taaattaccg tttaaataat
     3541 ctaccaattg agttatacta tctacgggaa ttatttctat accttctatt gctccgcatt
     3601 cttccttatt gtcttcaggc actattgctt tattaaagct cttgttccgc atggaaataa
     3661 ccattggtaa agcaccgtca atggcagtta tcctaccgtc caaggataat tctcctataa
     3721 tgcatatttt ttcatcaacg ggttttataa ttttggacgc agtaagtatg cccactgcaa
     3781 taggaaggtc tatttgactg ccttcttttt ttaagttggc aggtgcaaga tttaccgtaa
     3841 tacggcttac gggaaaatcg aatctgctgt ttttaattgc agagcgtact ctttcttttg
     3901 actctttaat tgaaatatca ggcaagccaa ctatattaaa gttaggaaga cctcccgata
     3961 tgtcagtttc cacatcgact gcataacctt caagtccgta aagaacacat gttttaattt
     4021 ttgaaagcat aatacctccg ataattgaag ttattaaatt atactataat tttccaatta
     4081 tttcaattaa tataaatctt taattatagt tttgcaataa aaatctcccc tttaacacct
     4141 attgcattaa agaggagaat acataaactt aggttaaaac gggaaataac gttgttccgc
     4201 ccaccattct tccttcaata cgttgtattg attggtgaat tggtctaaat ggactttttc
     4261 ccatctaatc agcaaatcga acagctcctt gcctgattcc gattttgctt tttccttggc
     4321 ttccttgtaa aaatccatgg agtccttctc catttgcatt cctatcctaa atatagacat
     4381 ttctaatgtt gcatatgctt tatcgacctt atcccagcga aaaatttccg gagaaggtat
     4441 ttcaattccg gaagccaata tatcttcaat ttttacttct ccgcctgcac tgagtatctt
     4501 ccataatctt tgtaaatatt ctgcatgctt caattcatcg tttgcaagag acataaatgc
     4561 ttcttttgta ccctgcgttt tagcttgctc tcctgccagc ttataaaatt cgtaaccttc
     4621 catttcattt aaaacagcct gtgaaataac ggccaattct tctttataca taaaattcct
     4681 cctttttatt attactatta taccattgga ggaatttctt aaacattatt ctttattctt
     4741 tcttatcgat agcatttttt actgcattca aaagtgcctt gctcgtaacc gtagctcccg
     4801 aaacagtatc gacatcggtt gtttgatttt ctattatgga agaaattatt gtgtctatag
     4861 catttgtacc aacaccttta gtttcattat ggtaaacaac ttcaatatcg gttattttat
     4921 catctttcat tttgactaag actttaatgg gtccgccgat acctctatcc tcgcctatgt
     4981 attcttccgg ctgtgaaatt atttcttcgg cctcctcgaa aggcactgtc tgttccggct
     5041 ctattaacgg ttcctcggta ggcactgtct gttccaactc cgctaatggc tcatcggcag
     5101 gtgcagaagt catatcattt tcggattctt catcgtataa gggctctttt gcagataatt
     5161 caggcaaagg ctcttcttct tcaggtattt gttgattttc ttcatgaatt tcttcagagt
     5221 tttcaattat ttgtttatca tccgatatac tgtcttcttt gatttctaat gcttcttcct
     5281 ttatttcttt atctatttct ttatccaagg agcatccgac aatagaaatt attaaaatta
     5341 ttattaaaat tgataggctt ttcatagtca cctcaagaaa aagcacctct tcttttttta
     5401 atggctgcca attccttgtt ttcttccgaa ataatttttt ctttttcaat atttaattca
     5461 ttagataaaa tgttaattaa aatattccta aaatccattt tccattctgt catagaaggg
     5521 ttgtaagtca atccaaaagc tacaagtctg cctgcatctt cttctatatg acccggcttt
     5581 acctgttcaa aatcaattcc ataaacttta ttatcgcaaa taataaaatt tttaaaattt
     5641 acatcatata atataaattg ttttttgtag aactcaaaaa ctgcagaata aaaatcttta
     5701 aaccaactgc aaagctcgta aaccatgctt atgttcaaag cattattttt ttcttgttcc
     5761 tcataccatt caagaaaatt aacttctccc aaatcttcca ggtatagata gttctcttct
     5821 gctttaatta ttgaaggaac attaactcct gcttttttta aaatacttag cacttctttt
     5881 tcccttatgt aattttcgtg gttctcaaat tttttcagaa tatatgttcc ttcatcttcc
     5941 actattcgaa atacattatt tcttttgctt tttagtttta tctctttatg catacttcga
     6001 tatctccgct ttccgtataa ccgtatagct cctttgcaat tgcttccact gtatttttca
     6061 aaatattctg aacaaacgga ttcatagcaa tttcctgtcc gttaatttta agggttacac
     6121 tgttttcagt caaaacgcaa tccctccttt ccgcctcgca ttttaaaatc tttgagcata
     6181 gttgagtgca tgtatatccg cattttttac agcattcatc cttcatgtcc ggaagtctgt
     6241 caaatacttt tttttcaatt aaatctacca gcttttcaat ttccgttaac gaatttatta
     6301 cgggtatacc ttcatattcc gttaaactgt ttgatatttg tcctgatatt gcaaatgttg
     6361 tttcatcaag cctttctatt attccttcca catcatgggc tgttactatt ttgggagcac
     6421 atgtatccct tactccttcc aagataacaa aatcatgatt gtaaaaacaa agaatttcat
     6481 tcatacttaa ttttctttga aacaaaacgt ctgtttcata ggcccctctt gccgtaacca
     6541 attgagagcc tgccatttta tgcctgtgag tgttagtccc gtcaacatcc attttaaatt
     6601 tctcaaaatg tatttctttt acactcccga cagtgtaatt acgtttccta agttccttta
     6661 ttatattttc tatggtagta gtcttgccgg attttgtcag tccgattact gaaaaaactt
     6721 tcataatgca cctctcgtta taataataaa caataatgct gctgaaatgc tcagccctaa
     6781 tagaatataa tcaaatttct gcatttttaa aaccaaatag cttgttcttt cggagtaagc
     6841 tctaaaacct ctcatctcca ttgctgcagc caattctttt gccttcataa tcgaattaac
     6901 ggttattgga gtaagtatat atttgtaaac ttgaaatttc ttactaagtt taaccttctt
     6961 caaatcaatt cctcttagtt gtattgccgt tattatat
//
LOCUS       DAOSOW010000047         6978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_10871_length_6978_cov_3.171024, whole genome shotgun sequence.
ACCESSION   DAOSOW010000047 DAOSOW010000000
VERSION     DAOSOW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10871_length_6978_cov_3.171024"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(125..643)
                     /locus_tag="PLD67_02165"
     CDS             complement(125..643)
                     /locus_tag="PLD67_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOT21365.1"
                     /translation="MARTKKEYLFIDGYNIINQWQYFRDISRNIENARNKLIELLVEY
                     QAYKGLKVVVVFDAHLVKGSREKKEYVSGVEVVYTKEHETADSYIEKQLDKIGRYEKG
                     RVATSDRAIQQIVLSRGGTRLTAQELILELENTKKDIRIKTENPSQKGANIDRILDTE
                     TLNKLDKIRKKI"
     gene            complement(646..1374)
                     /gene="rlmB"
                     /locus_tag="PLD67_02170"
     CDS             complement(646..1374)
                     /gene="rlmB"
                     /locus_tag="PLD67_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOT21366.1"
                     /translation="MRIIEGRNPVIEALRSDAEIDTILINKDAAQGSLNKIFELAKKK
                     NILIKNVDRALLDRLSENKKHQGVIAEAMEYKYKDIDDIFNYAKEKNEKPFIVILDEI
                     TDVHNLGSIIRSAECLGAHGVIIPKRRAAQINSVVAKSSAGAIEYLPVCRVTNISDTL
                     ETLKEKGLWIYGADMEGEKYIYEENFDVPVGLVIGSEGSGLGRLVKEKCDILIKIPMK
                     GNINSLNASNAASIIIYEIMKQRT"
     gene            complement(1371..1766)
                     /locus_tag="PLD67_02175"
     CDS             complement(1371..1766)
                     /locus_tag="PLD67_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HOT21367.1"
                     /translation="MKRTYMYSPAQLAYAGDAVYELLVRSYVLNNHDISVNKMHRLTV
                     RFVKADAQAYIISKLNDELTDEEKKIVKRGRNAKVTSSPKNAEFMDYRYATGFEALLG
                     YLFLNNDIERLMYIFEKVVEIIIEKDDSN"
     gene            complement(1805..>3208)
                     /gene="cysS"
                     /locus_tag="PLD67_02180"
     CDS             complement(1805..>3208)
                     /gene="cysS"
                     /locus_tag="PLD67_02180"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOT21368.1"
                     /translation="YNTLSRTIEDFKPIDENLVKMYTCGPTVYNYAHLGNLRMYIHED
                     VFEKTLRYLGYNVKRVMNITDVGHLESDADEGEDKMLKGAKRENKTVWEIAQYYTDAF
                     FDDIDRLNIKRPDVVAKATDYIDEYIEFIKVLEEKGYTYFANGNVYFDITKVKDYTKL
                     SGMDLDSLKSATREGVDLDVNKKNPHDFVLWFTKSKFENQAMKWDSPWGVGYPGWHIE
                     CSVISLCNLGEEMDIHCGGVDHIPVHHTNEIAQTESYTGKDWVKYWWHGEFLIDNEGK
                     MSKSSGEFLTLELLIKKGYNPLAYRYFVLNSHYRKQLAFSFDSIASAENAYNKLKSRI
                     SSLKNNADGIKENHEAYNKFKNDFENCLRDDLNTANAITVLYEMLKTDTLNNYEKLSL
                     IEDFDKVLSLDLLKEEEKDEVHDELSAYIEEMIQKRQEAKKNKDYQLADQIRAELLEK
                     GIMLEDTRQGVNWKKIN"
     assembly_gap    3209..3308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3309..3579)
                     /locus_tag="PLD67_02185"
     CDS             complement(<3309..3579)
                     /locus_tag="PLD67_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21369.1"
                     /translation="MKVPGTVASTVNGGAVTKDDSTPVPLSLCYFSTIFALKLLYLNK
                     KYWQIMLDTVSKVIYNKLSEEERIYMKIYNTLSRTIEDFKPIDENL"
     gene            complement(3613..4821)
                     /locus_tag="PLD67_02190"
     CDS             complement(3613..4821)
                     /locus_tag="PLD67_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOT21370.1"
                     /translation="MFGLVLEGGGARGAYHVGAYKAIRELNIEIGGIAGTSIGAINGA
                     MMVQGDYELLEKIWLSVDSYELFDLDEKTVEDLRHFNLRNINFSYLLHQSKEILSNRG
                     LDTSRIRELFDYYIDEEKIRSSNIDFGIVTVNLTDKKPMELMKEDIPEGKLIDFLIAS
                     ANLPAFRIEEMDGKKYLEGGFYNNLPIDILANKGYKNIIAVRTLAIGIIRKAKIKDLN
                     IIFIQPVESLGSMLGALDFNRERAEEYINLGYYDAMKVFKKLKGFKYYCIPFEGNFIN
                     ILTDFYNKYKERLCNIGRFLGYEDVCEDRMFFEKIIPRLESILDMKGKNDYQDICIRF
                     FERIAEKYGVERFKIYKAEEFVELTIEKFRENPTAFIKNVPNFIKQNRILSLAVKDDL
                     IVEIFAELFL"
     gene            complement(4831..5265)
                     /locus_tag="PLD67_02195"
     CDS             complement(4831..5265)
                     /locus_tag="PLD67_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003633855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOT21371.1"
                     /translation="MKKYLIVNKKILPEVYEKVIEARNLINTGSVKGISEAVKVVGIS
                     RSTYYKYKDYVFSPDENQIGRKAVISMMLKHEKGILSNVLNYLSQENANILTINQSIP
                     INNKASVNVSLDISDLSKSIDEVIADIKKIRGATQVKLLSVE"
     gene            complement(5353..6243)
                     /gene="thrB"
                     /locus_tag="PLD67_02200"
     CDS             complement(5353..6243)
                     /gene="thrB"
                     /locus_tag="PLD67_02200"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOT21372.1"
                     /translation="MVRVTVPATTANIGPGFDVLGLAFKLYNRITFERIEKGLIIKGC
                     PEVYNNNENLVYKSFVVTAENLGKKVDGLKISIDAKIPVSRGLGSSSACIVGGVYGAN
                     TFYKGGLTKDEMFKIAAAIEGHPDNISPCVYGGLTASMIEKERPYAVGYNISSTLKFY
                     ALIPDFETSTSEARKILPKTIDFKDAVFNVSRVAVLLKALETGDMDMINISLDDRLHQ
                     IYRGTLIHEYDNIREICAEAGSPALFISGAGPTLMNITKDEKFPEKIKDSISKLKHKW
                     RIEMLEPDKSGATVEVDSEK"
     gene            complement(6255..>6978)
                     /locus_tag="PLD67_02205"
     CDS             complement(6255..>6978)
                     /locus_tag="PLD67_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOT21373.1"
                     /translation="GDKVNFAVPTGNFGNILAGYYAKILGLPVNKLICASNENNVLYD
                     FIRRGVYDKNRDFHKTISPSMDILVSSNLERLLYYLSGKNNQIVKEYMKKLNTSGIYE
                     ISDEMAEKLREDFCGGCVFKEDAQKTIKDTFKKYGYLLDTHTAVAYKALEDYKTETED
                     DTVSVVLSTASPFKFAKSVYSSLFGETDDDEFVLMEELSEKTKIKIPENLRVLQYKKL
                     RHFEVCSVDEMEDFVISALNEY"
BASE COUNT         1964 a         1463 c          939 g         2512 t
ORIGIN      
        1 cgtatatagc gcgagaatta atcataatgt cctccaattc ataacatata aaataataat
       61 aaattatata ctgttataat caaaggatat acatattgag aaatcatgaa ttgttgtcaa
      121 agtgctaaat tttttttcta atcttatcaa gtttattcaa agtttcggta tccagtatcc
      181 tgtcgatatt agctcctttt tgactcgggt tttcggtttt tatccgaata tcctttttag
      241 tattttccaa ttccagaatc agctcttgag cagtgagcct tgtccctcct cttgacaaaa
      301 caatttgttg tattgctctg tctgacgtgg caacccggcc tttttcatac cttcctatct
      361 tatcaagctg cttttcaata tagctgtctg ctgtttcatg ctccttagta tagacaactt
      421 ccactcctga aacatactct ttcttttcgc ggctgccttt taccaagtga gcatcaaata
      481 caacaactac cttcagtcct ttgtatgctt gatattcaac tagaagctca ataagcttat
      541 tccttgcgtt ttcgatattt cttgaaatat ccctgaaata ctgccactga tttataatat
      601 tgtatccatc tataaaaaga tattccttct ttgttctagc catttttatg tcctttgctt
      661 cattatttca tatataataa ttgaagctgc attggaagca tttaaagaat ttatatttcc
      721 cttcatgggg attttaatca aaatatcaca tttttcttta accagtcttc ctaagcctga
      781 accctcgctt cctataacca accctaccgg aacatcaaaa ttctcttcat agatgtattt
      841 ttcgccttcc atgtctgctc cgtatatcca aagtcccttt tcctttaatg tttccaatgt
      901 atcacttata ttggtaaccc tgcagacagg aagatattca atagcaccgg cggaagattt
      961 tgcaacaaca ctatttattt gagcagctct tcttttggga attataacac cgtgggcgcc
     1021 taagcattcg gccgagcgaa taatagagcc taaattatgg acatcggtaa tttcatcaag
     1081 aatcactata aaaggctttt cgtttttttc ttttgcataa ttaaatatat cgtcaatatc
     1141 cttatatttg tattccatag cttctgctat gactccctga tgctttttgt tctcgcttaa
     1201 tctgtccaaa agtgccctat caacattttt aatcaatata ttcttttttt tagccagctc
     1261 gaaaatttta tttaaagagc cttgagccgc atccttattt atcagtatgg tatcaatttc
     1321 ggcatcgctt ctcaatgctt caattaccgg attccttcct tcgattatcc tcaattactg
     1381 tcatcctttt ctattattat ctcaacaact ttttcaaata tatacataag tctttcaata
     1441 tcattattta aaaacagata cccaagcaaa gcttcaaatc ccgtagcata tctgtaatcc
     1501 ataaattcag catttttagg agaagaagta accttggcat ttcttcctct ctttacgatt
     1561 ttcttctctt catcggtcag ttcatcattt aatttgctta taatataagc ctgggcatca
     1621 gcctttacaa acctgacggt aagcctgtgc attttattaa cgcttatatc atgattgttt
     1681 aaaacatagc ttcttaccag caattcataa acagcatcac ctgcatatgc cagctgtgca
     1741 ggtgaataca tatatgtcct tttcatttta tcgcttattt tattaagaac ttcaaattct
     1801 ttggttaatt tattttcttc caatttacac cctgtctggt atcttcaagc ataataccct
     1861 tttccaatag ttctgccctg atttggtcag ccagctgata gtctttattt ttctttgctt
     1921 cctgcctttt ttgaatcatt tcctcgatat atgcagacag ctcatcatga acctcatctt
     1981 tttcttcttc ttttaacaaa tccaatgaca agaccttatc aaaatcttct ataaggctta
     2041 acttttcata attgtttaat gtatccgttt taagcatttc atataaaacg gttattgcat
     2101 ttgcggtatt taaatcatcc cttaaacaat tttcaaaatc atttttgaat ttattataag
     2161 cttcatgatt ttcttttatt ccatccgcat tatttttaag ggagctgatt ctgcttttca
     2221 gtttattata agcattttca gcagaagcaa tagaatcaaa cgaaaaagca agctgctttc
     2281 tgtaatggga atttaaaaca aaatacctgt atgccaaagg attgtaacct ttcttaataa
     2341 ggagttcaag ggtcaaaaat tcaccgcttg acttactcat ctttccttca ttgtcgatga
     2401 ggaattcacc gtgccaccag tatttaaccc aatccttgcc ggtatagctc tctgtctgag
     2461 ctatttcatt ggtatgatga accggaatat ggtctacccc tccgcagtga atatccattt
     2521 cttcccctaa attgcagagg ctgataacgg aacactctat atgccatccg ggataaccga
     2581 ctccccatgg agaatcccac ttcattgcct gattttcaaa tttggatttt gtaaaccaca
     2641 aaacaaagtc atgagggttt ttcttgttaa catctaagtc aaccccttcc cttgtggcgc
     2701 tttttaaact gtccaaatcc attccggaaa gctttgtgta atccttaacc ttggttatgt
     2761 caaaatacac gttgccgttt gcgaaatacg tgtagccctt ttcttccaat actttgataa
     2821 actcaatata ttcgtctatg tagtcagtag cttttgcaac aacatcaggt cttttaatat
     2881 taagtctgtc tatatcgtca aaaaaggcat cagtataata ttgtgcaatt tcccaaacgg
     2941 ttttattttc tctttttgca cccttcagca ttttgtcttc gccttcatcg gcatctgatt
     3001 ccaagtgacc tacatcggta atgttcatta cccttttaac attgtaacct aagtatctga
     3061 gggttttctc aaaaacatcc tcgtgaatat acattcttaa attgcccaaa tgggcatagt
     3121 tgtatacagt aggtccgcaa gtgtacattt ttactaaatt ttcgtcaatt ggtttgaaat
     3181 cttctattgt tctggataat gtgttatann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnct aaattttcgt caattggttt gaaatcttct attgttctgg ataatgtgtt
     3361 ataaattttc atgtaaatcc gctcctcttc acttaactta ttatatatta cctttgaaac
     3421 agtatcaagc ataatttgcc aatatttttt gttaagataa agtaacttta atgcaaaaat
     3481 tgtcgaaaaa tagcacaatg acaaggggac gggggtgctg tcatcttttg tgacagcacc
     3541 cccgttaacc gttgatgcga cggtgccagg caccttcatg aggtgcctgg caccaagtgt
     3601 ttccccatac cactaaagga ataactcagc aaaaatttcc acaatcaaat catccttaac
     3661 tgctaatgat aaaattctgt tttgcttaat aaaattcggt acatttttaa taaaggcagt
     3721 aggattttct ctgaattttt cgattgtcaa ttcaacaaat tcttcagcct tatatatttt
     3781 aaatctttca acaccatact tttctgcaat tctttcaaaa aaccgaatgc atatgtcctg
     3841 atagtcattt ttacccttca tatccaatat tgattctaat ctgggtataa ttttttcaaa
     3901 aaacattctg tcctcgcaca catcttcgta gcccaaaaaa cgtccgatat tacataatct
     3961 ttctttgtat ttattataaa aatccgtcaa tatatttata aaattaccct caaaaggaat
     4021 acagtaatat ttaaagcctt ttagcttttt aaaaaccttc atggcgtcat aatagcctaa
     4081 attaatgtat tcctcagctc tttccctgtt aaaatctaaa gcgccgagca ttgagcctaa
     4141 gctttcaacc ggttgaataa agataatatt taagtctttt atttttgcct ttctgataat
     4201 accaattgcc agtgttctta cggcgataat atttttatat cctttgttgg ctagaatatc
     4261 tataggaaga ttgttataaa accctccttc taaatatttt ttcccgtcca tttcctctat
     4321 tctaaaagca ggaaggtttg cgcttgctat taaaaagtca attaattttc cctccggtat
     4381 atcctccttc ataagctcca tcggcttctt gtcggtcaga ttaacagtaa ctattccaaa
     4441 atcaatatta gaactgcgga ttttttcctc atcaatataa tagtcaaaca attctcttat
     4501 tcttgaagtg tccaaccctc tattgcttag tatttcttta gattgatgca aaagatagga
     4561 aaaattaata tttcgcaaat taaagtgcct caaatcttca actgtcttct cgtcaagatc
     4621 aaacaattca taggaatcaa cgcttaacca tatcttttcc agcaattcat agtccccttg
     4681 gaccatcatt gccccattaa tagcccctat ggatgttccg gcgattcctc cgatttctat
     4741 atttaattct cttatagctt tataggctcc tacatggtag gctcccctcg cgcctcctcc
     4801 ttctaaaacc aatccgaaca taatatcacc ctattcaact gataataatt ttacttgagt
     4861 tgcgcccctt atttttttta tatcggctat tacttcgtca atagacttgc ttaaatctga
     4921 aatatccaat gatacattta cagatgcctt gttgttaatt ggaatgcttt gatttatggt
     4981 aagaatattt gcattttcct gagacaaata attcaaaaca tttgagagta ttcccttttc
     5041 atgctttagc atcatcgaaa ttactgcttt tctgcctatt tgattttcgt ccggagaaaa
     5101 aacataatcc ttgtacttat aatatgtact cctgcttata ccaacaacct taactgcctc
     5161 gctgatacct ttcacacttc cggtgttgat taaatttctt gcttcaatta ccttttcata
     5221 tacttccgga agaatttttt tattgacaat caaatacttt ttcattatat ccacctccat
     5281 tcattattga ttattgcgca gttactattt taatgtcatt ctgagagtct tagctcgaaa
     5341 gatgccctaa ccctattttt cagagtcgac ttctactgtt gctccgcttt tatcgggttc
     5401 cagcatttca attctccatt tatgttttaa tttgctaatg ctgtctttaa ttttttccgg
     5461 aaatttctca tcttttgtaa tattcatcaa agtcggtcct gcgccgctta taaacaatgc
     5521 cggcgagcca gcttcagcac aaatttctct tatattatcg tattcatgaa ttaaagtgcc
     5581 tctgtaaatc tgatgcaatc tatcgtccaa tgaaatatta atcatatcca tatcgcctgt
     5641 ttccaatgcc tttagtaata ctgcaaccct tgatacatta aataccgcat ccttgaagtc
     5701 aattgtttta gggagaattt ttcttgcttc acttgtcgaa gtttcaaaat caggaattaa
     5761 ggcataaaac tttagtgtac tgcttatatt gtatccgacg gcgtaaggtc tttctttttc
     5821 aatcatggat gctgtcagtc ctccataaac acagggagaa atattatcgg gatgtccttc
     5881 aatcgcagcg gcaattttaa acatttcatc ttttgtcaat cctcccttgt aaaaagtgtt
     5941 agcaccataa acccctccca caatacatgc cgagctgctt cctaaacccc ttgaaacagg
     6001 aattttggca tctatggaaa ttttaagtcc atcaaccttt ttccctaaat tttcagcagt
     6061 aacaacaaac gatttataaa ctaaattttc attattatta taaacttccg ggcaaccttt
     6121 aattatcagt cccttttcaa ttctctcaaa agtaattctg ttatacagtt taaatgcaag
     6181 tcccaagaca tcaaagccgg gtcctatatt ggcagtagtt gccggaacag ttactctaac
     6241 catttttcac ctcattaata ttcatttaaa gcagaaatca caaagtcttc catttcatca
     6301 acactgcaaa cctcaaaatg ccttaatttt ttatattgaa gtactcttag gttttcaggt
     6361 atttttattt tagttttttc ggacaattct tccattaata caaattcatc atcatccgtc
     6421 tctccaaaaa gtgagctgta aacacttttg gcaaatttaa atgggcttgc agttgaaaga
     6481 actacgctta cagtatcatc ctcagtttct gttttataat cctcaagcgc cttatatgcc
     6541 accgctgtat gggtatcaag gagatatccg tattttttaa aggtatcctt gatggttttt
     6601 tgagcatctt ccttaaaaac acatcctccg cagaaatcct ctctcagttt ttcagccatt
     6661 tcatcggata tttcataaat acctgaagta tttagctttt tcatgtattc tttgactatt
     6721 tgattgtttt tgccgctcaa ataatacaag agcctttcaa gattgcttga taccaaaata
     6781 tccatggatg gtgaaatggt tttatgaaaa tctctgtttt tgtcataaac tccccttctg
     6841 ataaaatcat acagcacatt gttttcattt gaagcacaaa tgagcttatt aacgggaagc
     6901 cccagtatct tagcataata tcccgccaaa atatttccaa aatttccggt aggtactgca
     6961 aaattaacct tgtctcct
//
LOCUS       DAOSOW010000048         6841 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_11162_length_6841_cov_4.258473, whole genome shotgun sequence.
ACCESSION   DAOSOW010000048 DAOSOW010000000
VERSION     DAOSOW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6841
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11162_length_6841_cov_4.258473"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2450
                     /locus_tag="PLD67_02210"
     CDS             <1..2450
                     /locus_tag="PLD67_02210"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BpuSI family type II restriction endonuclease"
                     /protein_id="HOT21374.1"
                     /translation="YRVLHHQYTGALEMDYVVQNIVTGKYLCVVEVKRTPADVHSARY
                     QFQAMSYVQMNADQSEKPFYILTNLEYAFSFRYDANRPRVFQQMLEPGLSHIGRFDVD
                     EEDEFVEKLARYFSERLADYTNNTYEYLVTLEEFAAHMEQIKEEPKRWKTHLAVLLYE
                     YIRGAFTFINRNELRDIRLFRNDVSRICDEAARVNFKDIFNYSDESFESTVNIPNDTL
                     VDLYDFGNQNVNGDSVAGILHSIVSAGHEHDGEVPTDLELARIVAELAKYSSGELSDR
                     DLVCDPAAGSGNLISAAIPTYNLAPTQIVVNDVNSKLLELLSLRIGLNYANTISLQNS
                     PAIYNRNISEVEPLFFNDVKVVVMNPPFSAGINCVARKQPLYRSIRRLTGNEPRTNVG
                     QMPLEAVFLELLIELVQPGTTISCVFPKTHLMGRGPEAKAIRRLLLNQFGLHLVFTYP
                     GDEIFDDVTKDTCILVGKAKTSAEYVKVVSSYDKIPNIDIHRFTQTLSNDVADDFSPM
                     MPGVVAKKVSMQELVDEVEDGWRMLNSEMVDAITFVKEVFQSSEQFDELVELDYPTKR
                     GQAGNSGGSDIMFFDSRADLYEQFSDAGVVLSAGMRNAKLDSFDIGSGDSDFLDVSDN
                     SEDIVDAIIDAYNTLPDREGRQQRRRKTNQEWKKILNKESRGRFPANSVLIPRAIRTT
                     GRSYLSRNPVFVSTNFVVCSLPSLEMAIMMSTWMSTIFYQLICEVSSKDQEGMRKMEV
                     SDIEKTYVPKFDSISRETIDALSEECDAIEFLNLGRPQIREVDRIWARELFGDNADGL
                     LNKAQRMLEYLANRRNP"
     gene            2470..4356
                     /locus_tag="PLD67_02215"
     CDS             2470..4356
                     /locus_tag="PLD67_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT21375.1"
                     /translation="MAAAHRSFAEYVKKRFDNNFWAAAESYLEANLDSLGVELRRIHR
                     AGETEISDVKVEYVWVEDKPEMEIHFDVAVSIWFETHEGDYHYDDYDENIVWMMAHCR
                     GDLDKKLDDFEILRVSKYNGKSRVKDPMDDSLVPIIPYEKLDDVATAFLQEYYPAALR
                     IPMRGQDPVWVDPTELAKNMGLTVRNQRIREDGSVFGQIYFDDTDTVMYDANEDADVP
                     THIKGKTIVVDPQIYLLRNLGSFNNTIVHECVHWTKHRKAFKLEQLFNTDASYISCEV
                     VGDADSRISIKSTKYMEKQANQLAPRIQMPKEPFTIKAKEYIAYFMRQTDARHTVDVM
                     EMVIGALERDYGVSRQAVKIRLVELGFEEAIGTFNYVDGHYVKPHGFRKGAIKVNQTF
                     TLSAQDAAVQRFMNLELREKTANGDYLFVDNHFVYNAPLYVGYNEDGKLELTDYARAH
                     MDECCLAFDMEITSKVESTYHTACFLNREPSDVTFDITYHNGYQNAPQSRQVEMRKKQ
                     QAEWLEIRRQMTDDPEQCMDLLLDWRGMKYTDLGDAIDRDPKTISRTVKGTTNPSVET
                     AVLICFGMNLPPVISEKLLDVLNCKLKPMNPDHQWISEALHMKYPEPLWAVREYLEQY
                     GVNI"
     gene            4410..5438
                     /locus_tag="PLD67_02220"
     CDS             4410..5438
                     /locus_tag="PLD67_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21376.1"
                     /translation="MMGLTMLEMIDNRLAHFFPQPELYDVSPFSIENLRDCIIMFILK
                     DAYYLTEPKQSLRENRGTDADDVRMRDSRNIAYAQHYRDLQYNHVKNDLGIEIPELLS
                     ADVETMRGKLQGHNITPMQYFELNTLADHPLLKAIVNKRICDVKKISNATFLEYMQDY
                     DKLVSLLLKKLDGSDEDVIFGTIALFTLEWKYNVELFYSCAVNAEKVGVQDVPVHRLA
                     GLCAELSMPLAPDFTQRLHTESRFVLHRLKLVPTVYTASESDWDEVKDKICHYQTARY
                     YIEKEIVRKWDMAGFFARYTTREQWAKFFREHYDLRQIYVPKEWNNKCIRYMRSIYSA
                     MIKNQPTP"
     gene            5890..6378
                     /locus_tag="PLD67_02225"
     CDS             5890..6378
                     /locus_tag="PLD67_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT21377.1"
                     /translation="MRKFKTAEDNRTNYIYYFNDGTSYTITPGENGENATIISQLHGM
                     DDDELDAQRREAYHCPVHLDGYHDGDGDDADDRNPYLEDDTYNSLQQILTSIANEERS
                     LQMEKLKVALSELTDKQKDTIIKKFYRNMTNVQIAAEEGVSEAAIRNRLTKIYATLKK
                     KI"
BASE COUNT         2096 a         1234 c         1633 g         1878 t
ORIGIN      
        1 agtatagagt gttgcaccat cagtatacag gcgcacttga gatggactat gtggtgcaga
       61 atatagtcac aggtaaatac ttgtgtgttg ttgaagtgaa aagaactccg gctgatgtgc
      121 atagtgcaag atatcaattt caggcaatgt cttatgtgca gatgaatgca gaccagtcag
      181 aaaagccgtt ttatattttg actaatttag agtatgcttt ttcatttaga tatgacgcca
      241 acagacctcg tgtatttcag cagatgcttg agccaggtct ctctcacata ggacgatttg
      301 acgtagatga agaagatgag tttgtagaaa aacttgcaag atattttagt gagcgccttg
      361 ctgactatac gaacaatacg tatgaatatt tggttaccct ggaagagttt gcagctcata
      421 tggagcaaat aaaagaggag cccaagagat ggaaaacgca ccttgctgtt cttctttatg
      481 agtatataag aggtgcattt acattcatta acagaaatga gttaagggat attagacttt
      541 ttaggaacga tgtgtcaaga atctgtgatg aggctgcacg tgtaaatttt aaggatattt
      601 tcaactattc cgatgagtct tttgaaagca ccgtgaatat cccaaatgac acgttggttg
      661 atttgtatga ttttggaaat cagaatgtta atggtgattc tgttgcaggt attcttcatt
      721 ctatcgtgtc tgctggacat gaacatgatg gagaagttcc gacagatttg gaattggcaa
      781 gaattgtggc tgaattagca aagtatagca gtggcgaatt atcagacaga gatttagtat
      841 gtgaccccgc agcaggaagt ggaaacctga taagtgctgc aattcccaca tataatttgg
      901 caccaacaca gattgttgtt aatgatgtaa acagcaagtt gcttgaattg ctatctttga
      961 gaattggtct gaattacgca aacaccataa gtctacaaaa ttctccggcc atttataatc
     1021 gtaatatttc ggaagtggaa ccattgtttt ttaatgatgt taaggtggta gtaatgaatc
     1081 cacctttctc tgcgggaata aattgtgttg ctcgaaagca accgttgtat agaagtatta
     1141 gaagattaac aggaaacgag ccaagaacca atgttggaca gatgcccttg gaggcggtat
     1201 ttcttgagtt gcttatcgaa cttgtacaac ctggaacaac aatttcctgt gttttcccga
     1261 agacacattt aatggggcgt ggtccggaag ccaaggctat aagacggtta ctacttaatc
     1321 aatttggttt acatttggtt ttcacatatc caggagacga aatatttgat gatgtaacaa
     1381 aggatacgtg catccttgtt ggtaaggcta agacatctgc tgaatatgtg aaagttgtat
     1441 ccagctacga taaaattcct aatattgata ttcatagatt tacacagact ttgtcaaatg
     1501 atgttgcaga tgatttttca ccgatgatgc ctggagtggt agcaaagaaa gtttctatgc
     1561 aggagcttgt ggatgaagtg gaagatggtt ggagaatgct caacagcgaa atggttgatg
     1621 caatcacttt tgttaaagag gttttccaaa gttctgagca atttgatgaa ttggtagaac
     1681 tggattaccc tacaaagaga ggtcaggccg ggaacagtgg tggtagtgat atcatgttct
     1741 ttgattcaag ggctgacttg tatgagcaat ttagtgatgc tggtgttgta ctttctgctg
     1801 gtatgcgtaa tgctaaattg gatagttttg atattgggtc tggagatagt gattttttag
     1861 atgtttcgga taactctgaa gatatagtgg atgccattat tgatgcgtat aatacccttc
     1921 cagacagaga aggcaggcag cagcgtagga gaaaaaccaa tcaggagtgg aagaaaatct
     1981 taaataaaga aagtcggggt agatttcctg caaattctgt attgattccg agagcaattc
     2041 gcacaacagg aaggagttat ttatctcgta accctgtttt tgtttctact aattttgtcg
     2101 tttgttcatt gccgtcttta gaaatggcaa taatgatgtc tacatggatg agtactattt
     2161 tttatcagct aatatgcgaa gtttcctcta aagaccaaga aggtatgagg aaaatggaag
     2221 tttccgatat tgaaaagacg tatgttccta agtttgatag cataagcaga gaaaccattg
     2281 atgctctgtc ggaagagtgt gatgcaattg aatttttaaa cctcggaaga cctcaaatta
     2341 gagaagtcga cagaatttgg gcaagagagc tgtttggtga taatgcggat ggcttgctaa
     2401 acaaggcaca aaggatgttg gaatatcttg ctaacagaag aaatccgtaa taccaaggga
     2461 gggtagatta tggcagcagc acatcgttca tttgctgaat atgtaaaaaa gagatttgat
     2521 aataatttct gggcggctgc cgaaagctac ctggaggcaa accttgattc cttgggtgtt
     2581 gaattgagaa gaattcatcg tgccggagaa acggagattt cagatgtcaa ggtagaatac
     2641 gtgtgggttg aggacaagcc ggaaatggag atacatttcg atgtggccgt ttctatttgg
     2701 tttgaaaccc atgagggaga ttaccattac gacgattatg atgaaaatat cgtgtggatg
     2761 atggctcatt gccgtggtga tctggataag aaactggatg attttgaaat cctgcgggta
     2821 tcaaaataca acggcaaaag ccgtgtgaaa gacccgatgg atgattctct tgtgccgatt
     2881 atcccatatg aaaaattgga tgatgtggca acagcattct tgcaggaata ttatccggct
     2941 gcacttcgca ttccaatgcg tggtcaagac cctgtatggg ttgacccgac agaacttgca
     3001 aagaacatgg ggttaaccgt cagaaatcag cgtatccgtg aagatggttc tgtatttgga
     3061 cagatttatt ttgatgatac cgatacagta atgtacgatg caaacgagga tgcagatgtt
     3121 cctacgcata tcaaaggaaa aacaattgtt gtggacccgc agatttatct gctccgaaat
     3181 cttggttcat ttaataatac cattgttcat gagtgcgttc attggacgaa gcatcgcaag
     3241 gcattcaaac tggaacagct attcaatacg gatgcttctt acattagttg cgaggttgtt
     3301 ggcgacgcag attcccgcat atccataaaa tctacaaagt atatggaaaa gcaggctaat
     3361 cagcttgcac cgagaatcca gatgccgaag gaacctttta caataaaggc taaagaatac
     3421 atagcctatt tcatgagaca gaccgatgca aggcatacgg ttgatgtcat ggaaatggta
     3481 atcggggcat tggagcggga ttatggtgtt tcgagacaag ccgtaaaaat tcgattggta
     3541 gaacttggct ttgaagaagc catcggcaca tttaactatg ttgatggaca ctatgtaaag
     3601 ccacatggtt tccgcaaagg tgctatcaaa gttaatcaga catttacttt gagtgcccag
     3661 gacgctgcag ttcagcgttt tatgaatttg gaactccgtg aaaagaccgc aaacggcgat
     3721 tatcttttcg tggataacca ttttgtttat aacgctcccc tatatgtcgg ttataacgaa
     3781 gatggcaaac ttgaactgac cgattatgcc cgagcgcata tggatgaatg ctgtcttgct
     3841 tttgatatgg aaatcacaag caaggtagaa tccacatacc atactgcctg tttccttaat
     3901 cgtgagccga gcgatgtaac ctttgatatt acatatcaca acggatatca aaacgcgccg
     3961 caatcgcgcc aggtagagat gaggaaaaag caacaggctg aatggttgga aattcgcagg
     4021 cagatgacgg atgaccctga acagtgtatg gatttattgc ttgattggcg tggaatgaag
     4081 tacacggacc ttggtgatgc gattgaccgt gaccctaaaa caatcagccg aacagtgaaa
     4141 ggaacaacaa atccatctgt tgaaacagcg gttcttattt gctttggcat gaaccttcct
     4201 cctgttatca gtgaaaagct gttggatgtt ttgaattgca agttaaagcc tatgaatcca
     4261 gatcatcagt ggattagcga ggctttgcac atgaagtatc cggagccatt gtgggcagtc
     4321 agagagtatc tggaacagta cggtgtaaat atttaaaaat ttttttcata aaaaaacgga
     4381 catggcgtgt ccggtgaaat gggggtgaca tgatgggatt aaccatgctt gagatgatag
     4441 ataacaggtt ggcgcacttt tttccgcagc cggaattata cgatgtttcc ccgtttagca
     4501 ttgagaatct gcgtgattgt atcatcatgt tcattcttaa agatgcgtac tatttgactg
     4561 agccgaaaca atcgttaaga gaaaatcgag gaacggatgc tgacgatgta agaatgcgtg
     4621 acagcagaaa tattgcatat gctcagcatt atcgagacct tcagtataat catgtaaaaa
     4681 atgacttagg tattgaaata ccggagttgc tttctgcgga tgttgaaacc atgcgtggaa
     4741 aactgcaggg gcataatatt accccgatgc agtattttga attaaatacg cttgcagacc
     4801 atccactgct taaagctatt gtaaataagc gaatctgtga cgtaaagaaa atatccaatg
     4861 caaccttcct tgaatatatg caggattatg ataaattggt aagcctgctt cttaaaaaac
     4921 tggatggctc ggatgaggat gttattttcg gtacaatcgc cttgtttaca ttagaatgga
     4981 aatataatgt agaactgttt tattcctgtg cagtcaatgc ggaaaaagta ggggttcagg
     5041 atgttccggt gcatcgcctg gcgggattat gcgccgaact ttcaatgccg ttagcaccgg
     5101 attttacaca gaggcttcat acagaaagtc gatttgtcct acacaggctg aaactggtac
     5161 caaccgtata taccgcatcg gaatcagatt gggatgaagt aaaagataaa atatgccatt
     5221 atcagacggc cagatattat attgagaaag agattgtacg caaatgggat atggcaggtt
     5281 tctttgcccg atacaccaca agagaacagt gggcaaaatt cttccgggaa cactatgatt
     5341 taagacaaat ctacgttccg aaggaatgga ataataaatg cattcgctat atgagaagta
     5401 tttatagtgc gatgattaag aaccaaccta caccataatc ccgtttttcc gatttttcgt
     5461 tcttaaaagc cttcataaaa tttagataaa attaagccag aacatgagag taaatactct
     5521 tcgtgttctg gcttttttgc gttttacggc aatttttagc ggacacggcg tgtccatcgc
     5581 agaaaaaaat tccagtgtaa actataaata aaaacgcctg acattgagac gggtacacag
     5641 aaatgcgtat ccatctgagt gccagacact caaataaaaa atattccaat gcccgaagtg
     5701 gtcgacctta aggcggcggg atacatcaag agtcaaattc acggtatagc cgtggactga
     5761 ccaaagatgt acccaccgtg ctttgtcatg cccattttcg gtatcagagt cggtgtgtag
     5821 catcacatcg gctctttttg tgtccctgcc gccagccttt cgggcggaaa agaaaggcag
     5881 ggactttaaa tgagaaagtt caaaacagcg gaagacaacc gtacaaatta catctattac
     5941 ttcaacgatg gtacaagcta caccatcact cccggcgaaa acggagagaa cgcaaccatt
     6001 atctcacagc ttcacggcat ggacgatgac gaactggatg cacagcgtag agaagcatat
     6061 cactgtccgg ttcacttaga cggctatcac gatggggatg gcgatgatgc ggatgaccgc
     6121 aacccatacc ttgaggatga tacttacaat tctttgcagc agattttgac atccatcgca
     6181 aatgaggagc gttctcttca gatggaaaag ctgaaggtgg ctctttcgga actgacggac
     6241 aagcagaaag ataccattat taagaagttc tatcgcaata tgaccaacgt gcagattgca
     6301 gcagaagaag gtgtttccga ggcagccatc cgcaaccgct tgacgaagat ttacgcaacg
     6361 ctgaaaaaga aaatttaaaa aagtttgatt tgagggggtt cgattccccc tcatttttcg
     6421 cttatggaca gaggggtgag aaaaactcct cggaaaggag ccaagtgcaa tgggaattaa
     6481 acacaaggta tgtatcaaca ttgcaaaacc cggcggcaca ccttctccgg tagtccgaag
     6541 cggaacgatg cagattcgta agagaatact tgattttctc ttcggtcagc aggtaaacgt
     6601 cctggttctg tcacccggcg actcggtgca gactgtcgag attcacgaac tgaaaggagg
     6661 cgagaaccat gacaaacgca aacaagttta acctgctcct tgatgtggtg aagctgatgc
     6721 actcgttggc agacggactg gaggctgtgg cttatgcatt cgcagacagc caggaaatct
     6781 tcgttgaagc caaggaagtg tcgaagcctg tcgaaacagg acaacccaca aaacagatag
     6841 c
//
LOCUS       DAOSOW010000049         6805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_11244_length_6805_cov_4.308148, whole genome shotgun sequence.
ACCESSION   DAOSOW010000049 DAOSOW010000000
VERSION     DAOSOW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11244_length_6805_cov_4.308148"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1839)
                     /locus_tag="PLD67_02230"
     CDS             complement(<1..1839)
                     /locus_tag="PLD67_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014312026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOT21378.1"
                     /translation="MPHPVKIGNRVRMSYSKIKEVLELPDLIEVQKKSYEWFLNEGLK
                     EVFDDISPIEDYTGNLILEFVDHSLYSEPKFSQIECKERDATYSVPLKLKVRLINKET
                     GEIKEQEAFMGEFPLMTEKGTFIINGAERVVVSQLVRSPGVYYNMTRDKVGKELYDAT
                     VIPNRGAWLEFETDSNDIVYVRVDRTRKLPATVLVRAMDVSSNSELIDILGETEQLLK
                     TLEKDNTTNSEEALIEIYKRLRPGEPPTYDSAKSLIKSLFFDFKRYDLAKVGRYKLNK
                     KLSLENRIIGKRAAEDIINTETGEVLVTKDSIFDDESILKLEAAGIFSVKIVNSDDKV
                     TKVLSNKFVDPRVFELDKLGIDLEKIGIKEKVYYPVMKKILEENHSAEDLEKALIESL
                     KQVPAAENNIFEKDKEENYSISPKHIVKDDMYAIVNYHFGLFRGIGKTDDIDHLGNRR
                     LRSVGELLQNQVRVGLSRMERVVRERMTIQDVDVVTPQVLINIRPVSAAIKEFFGSSQ
                     LSQFMDQTNPLAELTHKRRMSALGPGGLSRDRASFEVRDVHHSHYGRMCPIETPEGPN
                     IGLITSLATYARINDYGFLESPYRRVDKKTGKVTDIIDYLTADVEDT"
     gene            complement(2235..2618)
                     /gene="rplL"
                     /locus_tag="PLD67_02235"
     CDS             complement(2235..2618)
                     /gene="rplL"
                     /locus_tag="PLD67_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOT21379.1"
                     /translation="MASEKITNFIEEIKKLTVLELNELVKAIEEEFGVSAAAPVAVAG
                     GAQAGAAAPAEEEQTEFDVVLTSAGAGKIKVIKVVRELTGLGLKEAKDAVDGAPTTIK
                     TGVSKEEAEKAKEALEAEGASVEIK"
     gene            complement(2767..3270)
                     /gene="rplJ"
                     /locus_tag="PLD67_02240"
     CDS             complement(2767..3270)
                     /gene="rplJ"
                     /locus_tag="PLD67_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOT21380.1"
                     /translation="MNQTVLEQKKQVVQEIAEKIRNAKSIVLVEYNGLTVDMANELRN
                     KCREAGVEYKVYKNTLMRFAFEELGYDGITPNLEGPNAIAFSYEDEVSAAKVTNDFAK
                     TSNDTIVIKAGIAEGRVMNASEVTALASVPTREILIAQLAGVLQGNIRNLAYMLDQIS
                     KKEGEAA"
     misc_feature    complement(3283..3399)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3427..4125)
                     /gene="rplA"
                     /locus_tag="PLD67_02245"
     CDS             complement(3427..4125)
                     /gene="rplA"
                     /locus_tag="PLD67_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOT21381.1"
                     /translation="MPKRGKKYQDSIKSIDRLKLYDMQEAVEAMLSTAKANFDETVEV
                     HLRLGVDSRHADQQVRGAVVLPHGTGKTVRVLVFAKGDKAKEAQEAGADFVGEAELAD
                     KIQKENWFDFDVVIATPDMMGVVGKLGRVLGPKGLMPNPKSGTVTFDVGKAIKEIKAG
                     KVEYRLDKTNIIHVPVGKKSFGAEKLIDNLKVILEAVIKAKPAAAKGKYLKSVTIAST
                     MGPGIKLNTTKLMD"
     gene            complement(4181..4606)
                     /gene="rplK"
                     /locus_tag="PLD67_02250"
     CDS             complement(4181..4606)
                     /gene="rplK"
                     /locus_tag="PLD67_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005956246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOT21382.1"
                     /translation="MAKKVTAVVKLQIPAGKATPAPPVGTALGPHGVNIMGFCKEFNA
                     KTADQAGMIIPVVLTVYQDRSFTFITKTPPVAVLIKKAVGIESGSGEPNKKKVATLSK
                     AKCEEIARLKMPDLNANTIEAAMSLVAGTARSMGVTVEK"
     gene            complement(4652..5179)
                     /gene="nusG"
                     /locus_tag="PLD67_02255"
     CDS             complement(4652..5179)
                     /gene="nusG"
                     /locus_tag="PLD67_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOT21383.1"
                     /translation="MKQESQGSRWYVVHTYSGHENKVKVNIEKLVENRGMQDTIFEVR
                     VPVEEVPEVKNGKKRIKERKMFPSYVIIKMIMTDESWYLVRNTRGVTGFVGPGSKPVP
                     LTDEEVKALGVIDRLPPIELEVGDTIKVKEGPFEDFIGTVESISQEKRKVKAFVSMFG
                     RETIIELDYDQIEKV"
     gene            complement(5192..5401)
                     /gene="secE"
                     /locus_tag="PLD67_02260"
     CDS             complement(5192..5401)
                     /gene="secE"
                     /locus_tag="PLD67_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009351089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOT21384.1"
                     /translation="MSNREEKEKFASKVKNYFKGVRSELKKVNWPTKKELTNYTIVVF
                     TTTLALTIVIWGLDLVFKGLLSVIV"
     gene            complement(5412..5561)
                     /gene="rpmG"
                     /locus_tag="PLD67_02265"
     CDS             complement(5412..5561)
                     /gene="rpmG"
                     /locus_tag="PLD67_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008538901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOT21385.1"
                     /translation="MRVAVKLACTECKQRNYDTEKNKKENPERIEMNKYCKFCKKHTL
                     HKETK"
     gene            complement(5862..>6805)
                     /gene="tuf"
                     /locus_tag="PLD67_02270"
     CDS             complement(5862..>6805)
                     /gene="tuf"
                     /locus_tag="PLD67_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOT21386.1"
                     /translation="GHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLARQVG
                     VPQLVVFLNKADMVDDPELIELVEMEVRELLTEYEFDGDNIPIIVGSALEALENPDSE
                     WGDKVIELMNAVDETVPTPARAIDKPFLMPVEDVFSITGRGTVATGRVERGVLKVQDK
                     IQIVELSDEPRDVVCTGVEMFRKLLDQAQAGDNIGALLRGVQRNEIERGQVLAKPGSI
                     TPHTKFKAEVYVLTKEEGGRHTPFFNGYRPQFYFRTTDITGQIDLEEGVEMCMPGDNS
                     RFIVELIHPIAIEEGLRFAIREGGRTVGSGVVTAIIE"
BASE COUNT         1799 a         1544 c          962 g         2500 t
ORIGIN      
        1 tgtatcttcc acgtctgcag taagataatc aataatatcg gttacctttc ctgttttttt
       61 atcaaccctt ctgtaaggag attctaaaaa tccgtaatca tttattcttg catatgttgc
      121 aagagaagtt ataagaccta tattaggacc ttcaggtgtt tcaatgggac acattctgcc
      181 gtaatgagaa tggtgaacgt ctcttacctc aaaactggct ctatctctgc ttaatccacc
      241 gggtcccaga gctgacattc ttcttttatg agtaagctca gccaagggat tcgtctggtc
      301 cataaactgt gacaactggc tggagccgaa aaattctttt attgctgcag acacaggtct
      361 gatatttata agaacttgtg gagttacaac atcgacatcc tgaatagtca ttctttctct
      421 gactactctt tccattcttg acaatcctac tctaacctgg ttttgaagaa gctcacccac
      481 ggagcgcagt cttctgtttc caagatggtc gatatcatca gtcttgccta tgcctctaaa
      541 taatccaaaa tgataattca ctattgcgta catatcatcc ttaacaatat gtttcggaga
      601 tattgaataa ttttcttcct tatccttttc aaatatattg ttttcagcag caggaacctg
      661 ctttaaggat tcaattaaag ctttttctaa atcctctgca gagtggtttt cttccagtat
      721 ctttttcatt accggatagt aaactttctc ttttattcct atcttttcta aatctatacc
      781 taatttatct aattcaaata cccttgggtc aacaaattta ttgcttaata cctttgtaac
      841 cttatcatcg gaattcacaa ttttaacgga aaaaatacct gccgcttcca attttaaaat
      901 tgattcatcg tcaaatatac tgtctttagt aactaatacc tctcctgttt ccgtattaat
      961 tatgtcttca gctgcacgct ttccaataat tctgttttcc aaactaagct tcttatttaa
     1021 tttgtatctg cctacttttg caagatcata ccttttgaaa tcaaaaaata aagatttaat
     1081 caatgacttc gcactatcat atgtaggtgg ctcgccgggt ctcaatcttt tatatatttc
     1141 tattaatgct tcttcggagt tggtagtgtt gtccttttcc aaagttttta aaagttgctc
     1201 tgtttccccc aatatatcaa tgagctctga attagaagaa acatccatag ctctcacaag
     1261 cacagtggca ggtaattttc ttgttctgtc aaccctaacg tatacaatat cattagagtc
     1321 tgtttcaaat tccaaccatg ctcctctgtt gggaataact gttgcatcat ataattcctt
     1381 accaacctta tccctcgtca tgttgtaata aacaccgggt gagcgaacta actgactgac
     1441 aacaacacgt tctgcaccat ttataataaa tgttcctttt tcggtcatca aaggaaattc
     1501 acccataaac gcttcttgct ccttaatttc tcccgtctcc ttgtttatca atcgcacttt
     1561 aagttttaga ggtacagaat atgttgcatc tctttcttta cactcaatct ggctaaactt
     1621 cggctcactg tataaagaat gatcaacaaa ctccagaatt aaattgccgg tataatcctc
     1681 tatcggagaa atgtcatcaa aaacctcttt cagcccttca tttaagaacc actcgtacga
     1741 ttttttctga acttcgatta agtccggcaa ttctaaaact tctttaattt tagagtaaga
     1801 cattcttacc ctattaccta ttttcacagg atgcggcatc ttttcacccc ttagtaaata
     1861 attttgactt acaaattcta tgccaacatt gcacactcat ggcaatatac aatactatca
     1921 ctaataaatt tttatgtcaa cacatttttt gcattattaa taaatatttt ttaacaattc
     1981 tctacaaatt ccgagtgatt tctttgtttg tatccatata aactttaaaa ccgccttctt
     2041 attatttgta aattttggta tattatgcat attataataa gctgagttcc tataccttaa
     2101 cataggtcgt aattattatg aataactttc atattgagag catagccctc atcattgcta
     2161 ttaaaataaa agaaactcta cttatgtaaa gtttctttct cgtttcgggt atactataag
     2221 tgtccctgca cttattattt aatttctact gaagcacctt ctgcttccaa tgcttcttta
     2281 gctttttcag cttcttcttt tgaaacgcct gtcttaattg tagtaggagc tccatcaact
     2341 gcatcctttg cttccttcaa tccaagacct gttaattctc ttactacctt tataactttg
     2401 attttaccgg caccggctga tgtaagaaca acgtcaaact ctgtctgttc ttcttctgcg
     2461 ggagctgctg cacctgcttg agctcctcct gctacagcta ccggagctgc tgccgatacg
     2521 ccaaattctt cttctattgc ttttactaat tcatttaatt ccaatactgt taattttttt
     2581 atttcttcaa taaaatttgt gattttttca ctagccattt tatattttcc tcctggtata
     2641 ataataatat atatttgtta tttttgtttt taaacgtcat cctgaacgtt agtgaaggat
     2701 ctcatcagtc agacttggta tatgagattc ttcgggctga agccctcaga atgacaaact
     2761 ttaggtttaa gctgcttccc cttctttttt gcttatctgg tcaagcatgt aagcaagatt
     2821 tctgatattt ccttgtaata cgcctgccag ctgtgcgata agaatttctc ttgtcggcac
     2881 gcttgccaat gctgtaactt cgctggcatt cataacccta ccttcagcta ttccggcctt
     2941 tattactatt gtatcattgc ttgtttttgc aaaatcattg gttacctttg ctgcagatac
     3001 ttcatcctca tatgaaaaag caattgcatt aggtccttcc aaattaggtg taatgccatc
     3061 atatcctaat tcttcaaatg caaaacgcat taaagtattt ttataaactt tatattcaac
     3121 acctgcttct ctgcacttgt ttctcaattc attagccata tctaccgtta agccattata
     3181 ctcaaccaaa acgatagatt tagcgtttct tatcttttca gcaatttctt gtactacctg
     3241 tttcttttgc tccaatactg tttgattcaa cttgttgcac ctcctttaaa caaaaaattc
     3301 tctccgcaga cgaagagaaa gaatctatta tctcacctcg gccggatatt taaactctca
     3361 tggagtaccg gctgtctgcg gtattatatt caatttttgc tttaatctgt ttgctttcgc
     3421 ttcagattaa tccattaatt tagtagtgtt aagcttgatt cccggtccca ttgtgcttgc
     3481 aattgtaaca ctctttaagt atttaccttt ggcagcagcc ggttttgctt taattactgc
     3541 ttcaagaata acctttaagt tatctattaa tttttctgca ccgaatgatt tctttcctac
     3601 aggaacgtga ataatgtttg ttttgtcaag tctgtattca actttacctg ccttaatttc
     3661 cttaatagcc ttacctacat caaaggtaac tgttcctgat tttgggtttg gcattaaacc
     3721 tttaggtcct aaaacccttc ccagttttcc gactacaccc atcatatcag gtgttgctat
     3781 aacaacatcg aaatcaaacc agttttcttt ttgaattttg tcagccaatt cagcttcgcc
     3841 cacaaaatct gctccggctt cctgagcttc cttagctttg tcgcccttag cgaaaaccaa
     3901 aacacgtact gtttttccag ttccgtgagg cagtactaca gcacctctta cttgttggtc
     3961 tgcatgtctt gaatctacac ctaatcttaa atgaacttca actgtttcat caaaattagc
     4021 ttttgctgtt gaaagcattg cttctacagc ttcttgcata tcgtataatt tcaatctgtc
     4081 aattgatttt atactgtctt gatacttctt tcctcttttt ggcattttat ttacctcctt
     4141 gtggtcaagc agccttctat cggcctccca cctgccataa ctatttttct actgttactc
     4201 ccatacttct tgcagtacct gccaccaggc tcatagctgc ttcgatagta ttagcattta
     4261 aatcaggcat ttttaatctc gcgatttctt cgcattttgc ctttgaaagt gttgcaactt
     4321 tctttttgtt tggttctccc gaaccgcttt caataccaac tgcctttttg attagaactg
     4381 caaccggcgg agtttttgtg ataaatgtaa atgatctatc ctgataaaca gtcaatacaa
     4441 ccggtataat catacctgcc tgatcggctg tttttgcatt aaactcttta caaaatccca
     4501 taatattcac accatgaggt cctaatgccg taccaaccgg tggagccgga gtagctttgc
     4561 cagcaggtat ttgcagttta acaacagctg ttactttttt tgccatattt gcacctcctt
     4621 catcgcataa cgatgttata ttgaaaagta attatacttt ctcaatctga tcataatcaa
     4681 gctcaataat agtttctctt ccgaacattg atacaaaagc tttgactttt cttttttcct
     4741 ggctgatact ttctacagtg ccaatgaaat cttcaaatgg tccttctttt actttgattg
     4801 tatctcctac ctcaagttca atcggcggaa gtctgtctat tacaccaaga gcctttactt
     4861 cttcgtcagt cagcggaaca ggttttgagc ccggacctac gaagcctgtt actccccttg
     4921 tgtttctgac aagataccaa gattcatcgg tcattatcat tttaataata acataactcg
     4981 ggaacatttt tctttctttt attcttttct tgccgttttt tacttcaggt acttcttcca
     5041 ccggcactct aacttcaaat atcgtatctt gcatccctct attttctaca agcttttcga
     5101 tatttacctt tactttgttt tcatgtcccg aatatgtatg aaccacatac catcttgacc
     5161 cttgtgactc ttgtttcata ccctactcct tttaaacaat aactgaaagc aagcctttaa
     5221 atactaaatc caatccccaa attactatag tcaatgctaa tgtagtagta aaaacaacaa
     5281 ttgtataatt tgttaattct tttttagtag gccaatttac tttttttaac tcagacctta
     5341 cgcccttaaa ataatttttt accttgcttg caaatttttc tttttcttct ctatttgaca
     5401 ttgccacacc tctattttgt ttccttatga agagtatgtt ttttacagaa cttgcaatac
     5461 ttgttcattt caattctctc aggattttct ttcttatttt tttctgtatc gtaatttctt
     5521 tgtttgcact cagtacaagc taatttcact gccactctca ttatttacac ctccaagaat
     5581 cttatacgca taagaaccca ttatcttact caagataaca caaatcaaat cgactgtcaa
     5641 taattttctc gatgaaatct acaaggaaat taacagttaa tttgtttcat ttcacttaaa
     5701 aaaagctcct gcccaagcaa aagcttttct tatgtagcaa ttaattttac aacagtaatt
     5761 ttaatttgtc aacacttaat tttcatttat gtcattcctg ttcgttgcca ggcaccaata
     5821 cctggtgcct ggcactgcga aaagaaccgt tcccttgtta tctactcgat tatagcggtt
     5881 acaactcccg aacctactgt tcttccgcct tccctgatag caaatctcaa accttcttca
     5941 atagctatcg gatggattaa ctcaactata aatcttgagt tgtctccggg catgcacatt
     6001 tcaacgcctt cttcaaggtc gatttgtcct gtgatatctg ttgttctgaa atagaactgt
     6061 ggtctgtatc cgttaaagaa tggagtgtgc cttccacctt cttctttagt caatacgtat
     6121 acttctgctt taaattttgt atgcggtgtt attgagcccg gttttgcaag aacttgacct
     6181 ctttcgattt catttctttg aactcctcta agaagggctc cgatattatc tcctgcttgt
     6241 gcctggtcaa gtaatttcct gaacatttca actccggtac atacaacatc tctcggttca
     6301 tcagacaatt ctactatttg aatcttgtcc tgaaccttca atactcctct ttcaactctt
     6361 cctgttgcaa ctgttcctct tcctgttatt gagaacacgt cttctacagg cattaagaac
     6421 ggcttgtcta ttgctcttgc aggtgttgga actgtttcat ccacagcatt cattaattca
     6481 ataactttat cgccccattc gctgtccggg ttttctaatg cttccagagc cgagccgact
     6541 attataggaa tattgtcacc gtcaaattca tattcggtta atagttctct tacttccatt
     6601 tcaaccaatt caattaattc agggtcatca accatatcag ctttgtttaa aaatactacc
     6661 aactgaggta ctccaacctg tcttgcaaga agaatatgct ctcttgtctg aggcatagga
     6721 ccgtcagctg ctgaaacaac caaaattgcc ccgtccatct gagctgctcc ggtaatcatg
     6781 ttcttaacat agtcagcgtg acccg
//
LOCUS       DAOSOW010000050         6789 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_11280_length_6789_cov_3.911197, whole genome shotgun sequence.
ACCESSION   DAOSOW010000050 DAOSOW010000000
VERSION     DAOSOW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11280_length_6789_cov_3.911197"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..1266
                     /locus_tag="PLD67_02275"
     CDS             235..1266
                     /locus_tag="PLD67_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOT21387.1"
                     /translation="MGLRMDIGIDLGTASVLVYIKGKGIVINEPSVVAIDINTNKILE
                     VGEEARKMLGRTPGNIVAIRPLRDGVISDFDITEKMLKYFIKKAVGNSFIKPRVIICV
                     PSGVTEVEKRAVLEASNNAGAKKTYLIEEPVAAAIGAGLDITKPSGHMVIDMGGGTTD
                     IAVISLGGIVVSRSIKVAGDKCDDAIVRYVRKKYNVMIGLRSAEELKIKIGTVFPVEE
                     ERFMEVRGRNLVTGLPVNLTISSSDSMEAMEETITTIADAVHSVLEKTPPELAADISE
                     KGIVMTGGGCLIHGMDKLLEKRTGLKVTIAEDAVSCVAKGTGQSLEHMDVLKDALITE
                     NIKMNYSIQ"
     gene            1284..2150
                     /gene="galU"
                     /locus_tag="PLD67_02280"
     CDS             1284..2150
                     /gene="galU"
                     /locus_tag="PLD67_02280"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HOT21388.1"
                     /translation="MKVRKAVIPAAGLGTRFLPATKAIPKEMLPIVDKPTIQYIVEEV
                     VASGIEDVLIITGRNKSSIEEHFDRAAELEYYLEKNNKEELLNEIKSISKMINIHTVR
                     QKDPLGLGHAIYCAKSFVGDEPFAVLLGDDVVDSQKPCLKQMLEVFEEYRSTILGVQP
                     VEWEKVHKYGIVSGSKINDRIYAVNGLVEKPGIENAPTNIAILGRYIITPKIFEILEN
                     TKAGVGGEIQLTDGLKELCNTEEIYAYIFQGRRYDVGSKIGFLEATVDYALKSDDLKE
                     GFKKYLRSINID"
     gene            2163..3875
                     /locus_tag="PLD67_02285"
     CDS             2163..3875
                     /locus_tag="PLD67_02285"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOT21389.1"
                     /translation="MYKEKFEMWLNSPHVDDVLKKELKEFEKIEIEIEDRFYRDLEFG
                     TGGMRGKIGAGTNRINLVTVSKATQGIANYLNEKYKGERISAAVAYDSRNMSKEFAEK
                     TACVFAANNIKVYIFDSLRPTPELSYAVRYFNCKAGVVITASHNPPEYNGYKVYDEFG
                     GQVVQDADKIIEKINEVKLEHIKSEEFIGNTKIQIVGNDVDTSYINDIKKLSLRNDVY
                     KNIKIIYTPLHGTGNIPVRRILDEMGYKNVFVVEEQEKPDSQFSTVSSPNPEELSSFE
                     IAIKKAKEMGGEIIIGTDPDCDRVGAIVKHKGEFIPLNGNQTGALLLNYLLASLDEQG
                     KIPDSPAVVKTVVTSEIANCIADKYNVTTFDTLTGFKYIAELMQKFEETKEYNFLFGY
                     EESFGYLAGTFVRDKDAVIASMLICEMAAYYKKQGKTLIDVLNEIYDEHGYYIEETVS
                     LNFSGVTGQSKMKAIMEHFRENKLSSLAGKKIPTIIDYKLSISLNLIKGTAERINYPK
                     SDVLKFSFEDGSWLVLRPSGTEPKLKVYFSINGHSRDESIESLEKIKSEILNIIDRIQ
                     INIG"
     gene            3906..5414
                     /locus_tag="PLD67_02290"
     CDS             3906..5414
                     /locus_tag="PLD67_02290"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT21390.1"
                     /translation="MKILKTTARKVILIIFVFVFTINTFVFAGVNPSREELELMIERV
                     AEKRAIPAILLKAIARVESCYEHYRADGSPKINGTSIGLMQINNKNGGYDSERLKYDI
                     MYNIEAGADVLLNKWAMSSYKEVASVGDMDPNVLENWYFALWAYNGWSQSNNPNIYQS
                     YTKKYTYQQLIYDVIEKEYGGKIHNIDFSYLPATGKPSRSLVVPTPMYTNGGNIIFYE
                     KGDYVRTDGIRTKFHLRDAPAGRYIHDLSLNQLGIIEDGPVLKNGYYWYKVYIDDNTE
                     GWIERNFLLRTGDAEYGRYVFEDISFHWARKIIMKLYGKNIVGEAEYFNPDKYVSKEE
                     FCILLNRALDYANVLNDSLGDDESIDDKDKEKSSRETETTSEEDASAILENIENINPW
                     AVEYVENVYESGLIDDEDLVNLLDNLNRKEAALIIANLFEISEEFSSLDIETVFTDIG
                     NLNEDEILAIKTVYTNGIMNGKGSGIFSPDANLTRAEAAVVMDKISERLSLQ"
     gene            5616..>6789
                     /locus_tag="PLD67_02295"
     CDS             5616..>6789
                     /locus_tag="PLD67_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOT21391.1"
                     /translation="MKTITRKIIMIFILLLIFNTTVYAGVNPSREEIEAIIDEVATKR
                     GIPAILLKGIARVESVFKHFNSDGSPKICGTSIGLMQINNVNGGYDNYRLKYDLLYNI
                     EAGADMLLNKWSMSSYKQVSSVGNMDPNILENWYFALWAYNGWSQSNNPVSPYAKKYT
                     YQQLVYDVIEKEYGKKINNIDFSYLPRESKPSRSLVVPTPNNYSSGNIIFYEKGDYVR
                     TDGIRKAYYLRDAPAGNYIYQLNVNQLGIITEGPLLKNGYYWYKVYIDETKEGWIERN
                     WLLRTGDTQYGRYIFEDIPFHWARKIIMDMYGRGIVSQAALYNPNNPVTYEEFCIFLS
                     RTLNYLENVNKQSKIQTSNKILYYDFTNVDLWALDFVKDIYERGLLKDIQPLQRLSR"
BASE COUNT         2508 a          937 c         1453 g         1891 t
ORIGIN      
        1 tactggatcc ggttcaaaaa atcaaaaaat tgcttaaaaa atagaatata caatgtctgc
       61 tttgcaggca tttttattgt agggcataac tcttatttat taatttattt tagtttgtgt
      121 attcctgtgc gatttccggg ataaaataaa ttgaaataaa ttaagttgta tgttataatc
      181 tttatcatta atattttaaa gataagataa taagaagaaa cgtggaggat gaagatgggc
      241 ctgagaatgg atatcggtat tgacctgggc actgccagtg tattggttta tattaagggt
      301 aagggaatag ttataaatga accttctgtt gtggctattg atattaatac taacaaaatc
      361 cttgaggtgg gagaagaagc aagaaaaatg cttggacgta ctccgggcaa tattgtagca
      421 attagaccct taagagatgg tgttatatct gatttcgata ttacggaaaa aatgttgaaa
      481 tattttataa aaaaagcagt aggaaattca tttataaaac cgcgagttat aatttgtgtt
      541 ccaagcggtg ttacagaagt tgaaaaaaga gcagttcttg aagcaagtaa taacgcaggt
      601 gcaaaaaaga catatcttat tgaagagcct gttgcagctg ccataggagc aggtctagac
      661 ataacaaagc catccgggca catggttata gacatgggcg gaggtacaac ggatattgct
      721 gttatatctc ttggaggcat agttgtaagc aggtctatca aggttgccgg tgataagtgt
      781 gacgatgcga ttgtaagata tgtacgaaag aaatacaatg tcatgatagg tctaagaagt
      841 gcagaagagc ttaaaataaa aataggaacg gtatttcctg tagaagaaga aagatttatg
      901 gaagtgagag gcaggaattt ggttaccggt cttccggtta atttgactat ttcatcttca
      961 gattctatgg aggctatgga agaaacaatt acaacaattg ctgatgcggt acattcagtt
     1021 cttgaaaaaa ctccgccgga gcttgcagct gatatcagcg aaaagggtat tgtaatgaca
     1081 ggcggaggat gcctcattca tggtatggat aaactgcttg aaaagagaac cggattaaaa
     1141 gtaaccatag cagaggatgc cgtatcttgt gtagccaaag gaacaggaca gtctttggag
     1201 catatggatg ttctaaaaga tgcattgatt actgaaaata taaaaatgaa ttacagcatt
     1261 caatagctga agcagaggta aacatgaaag ttagaaaggc agtaatacct gcagcagggt
     1321 tgggcactag atttttgcct gcaacgaaag caattcctaa ggaaatgctg cccattgtgg
     1381 ataaaccgac aatacaatac atagtagaag aagttgttgc ttccggaatt gaagatgtat
     1441 taataataac aggaagaaac aagagttcaa ttgaagagca ttttgacagg gcagcagagc
     1501 ttgaatacta cttagagaaa aataataagg aagagctttt aaatgaaatt aaaagtattt
     1561 ccaaaatgat aaatattcac acagtaaggc aaaaagaccc tttggggtta ggtcatgcaa
     1621 tttactgtgc gaaatccttc gttggcgatg agccctttgc agtgctctta ggtgacgatg
     1681 ttgttgattc acaaaagccg tgcctaaagc aaatgttgga agtttttgag gaatatcgct
     1741 ctacaatact tggtgtgcaa ccggtagagt gggaaaaagt ccataaatac ggcatagtgt
     1801 caggcagtaa aatcaatgac aggatttatg ccgtgaatgg tttggttgaa aaacccggta
     1861 tagaaaatgc acctacaaat attgcaatat taggaaggta tataataact ccgaaaatat
     1921 ttgaaatact tgaaaatacg aaagccggtg tggggggaga aattcagctt acagacgggt
     1981 taaaggaatt atgtaatacc gaagaaatat atgcttatat attccagggc agaagatatg
     2041 atgtaggcag taaaatcggc tttcttgaag caactgttga ttatgctctc aaaagtgatg
     2101 acttgaaaga aggctttaag aagtatttaa ggtcaataaa catagattaa ggggtggggg
     2161 aaatgtataa agaaaaattt gaaatgtggc ttaacagtcc acatgttgac gatgtgctaa
     2221 aaaaagaatt aaaagaattt gaaaaaattg aaatagaaat agaagacaga ttttataggg
     2281 acttggaatt cggtaccgga ggtatgagag gaaagattgg tgcaggaact aatcgcataa
     2341 atttggttac cgtttctaaa gcaactcagg gaattgcaaa ttatttgaat gagaaataca
     2401 aaggtgaaag gatttcagca gctgttgcat atgactcaag aaatatgtca aaggagtttg
     2461 cagagaaaac agcatgtgta tttgctgcaa ataacataaa agtttatatt tttgacagcc
     2521 ttagaccaac tcctgaatta tcttatgctg tgaggtattt taattgcaag gcaggcgtag
     2581 taataactgc aagccataat cctccggaat ataacggtta caaagtatac gacgaatttg
     2641 gaggacaggt ggttcaagat gctgataaaa ttattgagaa aattaatgaa gtaaaattag
     2701 agcatataaa atcagaagaa tttattggaa atacaaagat tcaaatagta ggaaatgatg
     2761 ttgatacatc ttatataaat gatataaaga aactaagctt aagaaatgat gtttataaaa
     2821 atataaaaat catatacact ccccttcacg gtacaggaaa tataccggtg agaagaattc
     2881 tggatgaaat gggatacaaa aatgtatttg ttgttgagga gcaggaaaaa ccggattctc
     2941 aattttcaac ggttagttca ccaaaccctg aagaactgag ctcttttgaa atagctataa
     3001 aaaaagctaa agaaatgggc ggtgaaataa ttataggtac tgaccctgac tgtgataggg
     3061 taggagcaat agtaaagcat aagggagaat ttattccttt aaacggaaat caaaccggag
     3121 cattgctttt aaattacttg cttgcttcat tggacgagca agggaaaatt cctgatagtc
     3181 cggcagttgt taaaactgtg gtaaccagcg aaattgcaaa ctgcattgca gataaatata
     3241 atgtaacaac ttttgacaca ttgaccggtt ttaaatatat tgccgaattg atgcagaagt
     3301 ttgaagaaac aaaagaatat aattttttgt ttggatatga agaaagtttc ggctatttgg
     3361 caggaacatt tgtaagagat aaggatgcag tgattgcttc tatgctcatt tgtgaaatgg
     3421 ctgcatatta taagaaacaa ggtaagacat taatagatgt attaaatgaa atatacgatg
     3481 aacacggtta ctatatagaa gaaactgttt cactgaattt ttccggtgtt accggacaaa
     3541 gcaaaatgaa agcaattatg gagcatttca gagaaaacaa actgagttcc ttagcgggta
     3601 aaaagattcc gacaattatt gactataagt tgagtatttc attaaacctt ataaaaggta
     3661 ctgctgaaag aataaattat ccgaaatcgg atgtcctgaa gttttctttt gaagacggct
     3721 cttggcttgt attgaggccc tcaggaacag agcctaaatt aaaggtgtat ttttctatta
     3781 acggacatag ccgggatgaa tcaattgaaa gcttagaaaa aataaaaagc gagatattaa
     3841 atataattga ccgcattcaa ataaatatcg gataagacta aaatctaaat agggaggctg
     3901 taaatatgaa aatattgaaa accactgccc gaaaagttat tttaataatt ttcgttttcg
     3961 tatttacaat aaatacattt gtatttgcgg gagtaaatcc atccagggaa gaactggagt
     4021 taatgattga gagagttgca gaaaagagag caattccggc aattctttta aaagcaatag
     4081 caagagttga atcttgttat gagcattaca gagcagacgg cagtccgaaa ataaatggta
     4141 caagcatagg ccttatgcag attaacaaca aaaatggagg ctatgacagc gaaaggttga
     4201 aatatgacat aatgtataat attgaagctg gagctgatgt actcctgaat aaatgggcta
     4261 tgagttcata caaagaagtt gcaagtgtag gcgatatgga ccctaatgta ttggaaaact
     4321 ggtatttcgc tttatgggct tacaatggct ggtcacaaag caacaatcca aatatatatc
     4381 aatcatatac aaaaaaatac acttatcagc agttgattta tgatgttatt gaaaaagaat
     4441 atggcgggaa aatacacaat attgattttt catatcttcc tgcaacaggg aaaccgagca
     4501 ggtccttggt tgttcccaca cctatgtata caaacggcgg taacataata ttttatgaaa
     4561 agggagatta tgttagaact gacggtataa ggacaaaatt tcatctgagg gatgctcctg
     4621 caggtaggta tatacatgat ttatccttaa atcaattagg tattatagaa gacggaccag
     4681 tgttaaaaaa cggatattat tggtacaagg tatatataga tgacaatact gaaggctgga
     4741 tagaaagaaa ctttctactt cgaaccggtg atgctgaata tggcagatat gtatttgaag
     4801 atatatcctt tcactgggca agaaaaataa taatgaagct atacggaaaa aatattgtcg
     4861 gagaagcaga atatttcaat cctgacaaat atgtttcaaa agaagaattc tgcatacttt
     4921 taaacagagc gttggactat gctaatgttc tgaatgacag cttaggagat gatgaatcta
     4981 tagacgacaa agacaaggaa aaaagctcac gggaaacaga aacgacatct gaagaagatg
     5041 catccgctat attagaaaat attgaaaata ttaatccttg ggctgttgaa tatgtagaaa
     5101 atgtttatga gtcgggatta atcgatgatg aagatttggt taatcttttg gacaatttaa
     5161 atagaaaaga agcagcttta attattgcaa atttattcga aatcagcgaa gagttttctt
     5221 cattagatat agaaactgta tttaccgata taggcaattt gaatgaagat gaaattctgg
     5281 caataaaaac ggtctacacc aacggcatta tgaacggtaa aggttcaggg atattcagtc
     5341 ctgatgccaa tttaaccaga gctgaggctg ccgttgtaat ggataaaata tccgaaagat
     5401 tgtcattgca ataaaaaaac cgtagattgt cattctgaac gtagtgaaga atctcaaatt
     5461 ggggatagta cttcttaaaa gaggtactat ccccttcgtt tttatattgg tacgaataaa
     5521 tgcataccta aaaggtataa ttttcaataa tatgggaata tttacattat aaacaagatt
     5581 attgtgaatt tcacaaaaac agagaggtgt aaatattgaa aaccataacc cgcaagatta
     5641 taatgatttt tatactttta ttgatattta acacgacagt atatgcaggt gtaaacccat
     5701 ctagggaaga aatcgaagca attattgatg aggttgcaac aaaaaggggt ataccggcaa
     5761 tattattaaa aggaatagca agagtcgagt ccgtgtttaa gcattttaac agcgacggaa
     5821 gtcctaaaat atgcggaaca agcattgggc tcatgcagat aaacaatgta aacggaggct
     5881 atgacaacta cagacttaag tatgatttat tgtacaatat agaagcaggt gcagatatgc
     5941 ttcttaacaa atggagcatg agttcataca aacaagtgtc aagtgtagga aatatggacc
     6001 ccaatatttt ggaaaactgg tattttgcat tatgggcata caacggttgg tcacaaagca
     6061 acaatcccgt ttcaccttac gccaagaaat atacatatca gcagcttgtt tacgatgtta
     6121 tagaaaaaga atacggtaaa aaaataaata atattgattt ttcttatctt ccgagggaaa
     6181 gtaagccaag caggtcattg gtagtcccga cacctaataa ctattcaagc ggtaatatta
     6241 tattttacga aaaaggagat tacgtaagga cagacggcat caggaaggct tattatttaa
     6301 gggatgctcc tgcaggaaat tatatatatc agctaaatgt taaccagttg ggaataatca
     6361 ccgaaggtcc cctactaaag aacggatact actggtataa agtatacata gatgaaacaa
     6421 aggaaggctg gattgaaaga aattggcttc tgcgtacagg ggatacacaa tacggcagat
     6481 atatatttga ggatatacca ttccactggg caagaaaaat tattatggat atgtatggaa
     6541 ggggaatagt aagccaagcg gcactatata acccgaataa tcctgtaaca tatgaagaat
     6601 tttgtatatt tttgagcagg actttaaatt atttggaaaa tgtcaacaag caaagcaaaa
     6661 tacaaacatc aaacaaaatt ctctattatg attttacgaa tgtagactta tgggcattgg
     6721 attttgtaaa agacatttac gagcggggtt tattgaaaga tatacaacct ttacaaagac
     6781 tcagtagaa
//
LOCUS       DAOSOW010000051         6682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_11527_length_6682_cov_3.733213, whole genome shotgun sequence.
ACCESSION   DAOSOW010000051 DAOSOW010000000
VERSION     DAOSOW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11527_length_6682_cov_3.733213"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..942
                     /gene="ftsE"
                     /locus_tag="PLD67_02300"
     CDS             247..942
                     /gene="ftsE"
                     /locus_tag="PLD67_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HOT21392.1"
                     /translation="MIEFNNVTKIYKKTFTAVKNVNLKIDKGEFIFIVGKSGAGKSTL
                     IKLLLREISPSEGIITYNNQDINKIRKRSVAEYRRRIGFIFQDYRLLPKMTVYENIAF
                     AMEMICCQAKNIRREVPLVLSMVGLSDKAKYYPDELSGGEQQRVAIARAIINKPETVI
                     ADEPTGNLDMETSKEIMKILTEINRRGTTVIMATHDREIVNQYQRRVVELKNGIVIRD
                     IKAGGYSYESEED"
     gene            923..1828
                     /gene="ftsX"
                     /locus_tag="PLD67_02305"
     CDS             923..1828
                     /gene="ftsX"
                     /locus_tag="PLD67_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOT21393.1"
                     /translation="MNPKKISYIFRQAFKSMWRNRMMGFASIGSVSAVLIILGFVLIL
                     VLNINNMAMVAKETFDQIAVYIMEGVDDEQIEEMGNSFRKIDGVMGVAFQTKDYALEK
                     VKEDWGEDAILLQDLRHNPFPNTYIVQLSDVSKADKVIAQLQTFEGVDKVRYYQDAVN
                     TLIKLADFVKKFGAGIIIILLLISVFIISNTIKITVLSRQREIELMQYIGATNGYVRG
                     PFILEGVMLGLLGSVLAILLIMLGYNYTIDYLAGRYVALISGMSGYLVGVEMVLYDLI
                     IIFMTIGIGIGVLGSLVSLKKFLSI"
     gene            1839..2963
                     /locus_tag="PLD67_02310"
     CDS             1839..2963
                     /locus_tag="PLD67_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOT21394.1"
                     /translation="MFRKCVAVILLIITIIGIFPANAGGSIADLRYKQKQIEQQIKDI
                     RKKAEELKGEKKSIEAEIQILDSELRALTLETEKQEVQKQELNMRIAESEQKIAELTD
                     EIDKNNMLLEQRLRAMYMNGTGGYLEVILNAENLIDALTRMDLIQLIVKSDVELLKTI
                     NEQKKEVEELKAAQEIRRHELTAVINDLNIKQNEVLIASRSKENYMSSLQNNIEEILR
                     QEAAMEEQSKQIEKDIIALQRAVEYAGGELLWPLPGHYRITSEYGGRIHPITGVWSTH
                     GGTDIAAPYGTNILSSNDGVVIYAGYHYSYGNYIIVDHGGGIATLYAHCSKLLASEGQ
                     AVTKGEPIAKVGSTGESTGNHLHYEVRLNGVRKNPMEYLK"
     gene            3035..4072
                     /locus_tag="PLD67_02315"
     CDS             3035..4072
                     /locus_tag="PLD67_02315"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOT21395.1"
                     /translation="MRIGIVGFGGVGRAFLNLIYNKKNKLESEGIYFQVNYIIGRTGG
                     VYNKHGIDLHKFINFLFDEKDITKYCDGGKAEDINFELMLNNKDIDLMVEMTPTNKET
                     GEPGMTHITRCLENNINVVTSNKGPILLAYHKLYNLAKANKVQLGIGCTTGGALPSIN
                     GGFIDLAGADIISIEGVLNGTTNFILKEMEDTGCNYDDALKEAQRLGIAETNPALDVE
                     GFDTASKLLILTNVLMNTEKTMDDIFIEGITKLTPHDINRAKSMNKKYKLVGKTQILD
                     NKIEMEVKLQLLDPSNPLYGVEGKNKAVRYISDTLGELTIMGGASGVTPAAASILRDI
                     ININRGYKFVK"
     gene            complement(4193..5743)
                     /locus_tag="PLD67_02320"
     CDS             complement(4193..5743)
                     /locus_tag="PLD67_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT21396.1"
                     /translation="MKIKYFPLIIFFIVYILLFATLPQNIKLPLLYFTPLLLYTMFIS
                     KAMFYYMKNKKQDDDMEVNEIASTVTYFDKKKIDEQKIQISFNDVAGLEEIKEDLMDI
                     IDFLNNEDKYRLMDAKVPRGILLYGPPGTGKTLIAKAIAGEAKATFIYSSGSEFVEKY
                     VGVGAKRVRTIFEKARKEAPSIIFIDEVDALGAKRNADSNNEKDQTLNQLLIELDGFN
                     NTSNVIVIAATNRLDLLDEALLRPGRFDRKIYVGNPNFHSRLKILEVHTKNKPLDKKV
                     QLKDIAIKTHGFSGAQLANIANEAALKAIKEKSKKITNENFEFAIEKIAAGLEIKHST
                     VTDKEKRIVAYHEAGHAVAAKILNIDQVQKISIIPRDKALGYVLKFPTEDKFLYTKNE
                     LCNKVIVLLAGRASEEIFIGEISTGASNDIKESTNIIYEIICNYGMGKNTQNTCIDER
                     FIKYYLDNIKDEAQEMTKEAYESAMAIIRENEAVVDKIAQYLLKYETMNAEKLDELMD
                     ETKKTALI"
     misc_feature    5874..5997
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            6097..6606
                     /gene="infC"
                     /locus_tag="PLD67_02325"
     CDS             6097..6606
                     /gene="infC"
                     /locus_tag="PLD67_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOT21397.1"
                     /translation="MIKELQINEQIREKEVRVVDSDGSQLGIISTKEALKIAESKKLD
                     LVNVSPNSDPPVCRIMNYGKYKYAQAKKEKESKKKQKVISVKEIRMTPNIEEHDLAVK
                     AKNASKFLIDGDRVKVVVRFRGRELGKMDRGREVLMQFAEMTSEYSTIDKHPVVEGKN
                     MSMFLTPKK"
BASE COUNT         2287 a         1009 c         1331 g         2055 t
ORIGIN      
        1 ttttatgaga tttcatgcaa atctcgtaaa acagtctttt ttttatagcc cgaaccctaa
       61 ttatcatatg aaacctgcaa tgaataaccg tatttttata gtgaacacat ttaggattac
      121 atatattttt atatatgtta aaaataaaca tttcattttt tttaaaaata aagtaaaata
      181 ttataataat agttgaaatt ttgtcatttt gtgttatatt aatattatac gctatgaggt
      241 gaaacagtga ttgagtttaa taatgttaca aagatatata agaaaacttt taccgctgta
      301 aaaaatgtca atttaaaaat tgataaaggc gaatttatct tcattgtagg aaaaagcgga
      361 gcaggtaaaa gtaccttaat aaaattattg cttagggaaa taagtccttc ggaaggaatt
      421 attacatata ataatcagga tattaataaa atcagaaaaa gaagcgtggc ggaatacaga
      481 aggcgtatcg gctttatttt tcaagactac aggctgcttc ctaagatgac tgtatatgaa
      541 aatattgcat ttgccatgga aatgatttgt tgtcaggcaa aaaacattcg aagagaggtt
      601 ccactggttt tgagcatggt gggattaagc gataaggcca aatattatcc cgatgaattg
      661 tcaggaggag agcagcagcg tgttgctatt gcaagagcta ttattaataa acctgaaacc
      721 gtaattgcag acgagcctac aggtaatttg gatatggaaa catctaagga aataatgaaa
      781 atattgacgg aaataaacag gcgaggaact acggttatta tggcaacaca cgacagagaa
      841 atagtaaatc agtatcaaag aagagtagtt gagcttaaaa acggtattgt tatcagagac
      901 atcaaggcag ggggatacag ctatgaatcc gaagaagatt agctatatat tcagacaggc
      961 atttaaaagt atgtggcgaa atcgaatgat gggttttgct tccataggct cagtctcggc
     1021 agtactgata attctcggat ttgttttaat actggtatta aatataaata atatggcaat
     1081 ggttgccaag gaaactttcg accaaattgc agtatatata atggaaggcg tggatgatga
     1141 gcaaatagag gaaatgggta actccttcag aaaaatcgac ggagtaatgg gagttgcatt
     1201 ccaaacaaag gactatgctc ttgaaaaggt taaggaagat tggggcgaag atgctatttt
     1261 actgcaggat ttaagacaca atccttttcc aaacacatat attgtacagc tttccgatgt
     1321 aagcaaggca gataaagtaa ttgcacaact gcagactttt gaaggtgttg ataaggtaag
     1381 gtattatcag gatgccgtaa acacccttat aaagttagct gattttgtta aaaaatttgg
     1441 agcaggcata ataataatac ttttattaat aagtgtattt attatatcca atacaatcaa
     1501 gattacggtt ttaagcagac aaagggaaat tgaacttatg caatatatcg gagctaccaa
     1561 cggatatgtc agaggacctt ttatactgga aggtgtcatg ttgggactat tgggctctgt
     1621 attggcaatt ttacttatta tgcttggtta taattatacc atcgattatc tcgccggtcg
     1681 ttatgttgca cttatatcgg gaatgtcagg atacttggtc ggcgttgaaa tggtactgta
     1741 tgaccttatc ataattttta tgacaatagg aataggtatc ggagtattag gaagcttggt
     1801 ttcattgaaa aaattcttga gcatttaagg agtgtattat gtttagaaaa tgtgtcgctg
     1861 ttattctgtt aataataaca ataataggta tttttccggc aaatgcagga ggaagtattg
     1921 ctgatctaag gtataagcag aaacaaatag agcagcaaat caaagacata cgtaaaaagg
     1981 cagaagaact taaaggcgaa aagaaaagta ttgaagctga aattcaaatt cttgacagtg
     2041 agctcagagc attaactctg gaaaccgaaa aacaagaagt tcaaaagcaa gagctaaata
     2101 tgcgaatagc agaatcagag caaaaaattg ctgagctgac tgatgaaata gataagaata
     2161 atatgctttt ggagcaacgc ttaagagcta tgtatatgaa cggaacaggc ggatatctgg
     2221 aagtaattct caatgccgaa aaccttatcg atgcattaac aagaatggat ttgattcaat
     2281 taatcgttaa aagcgatgtt gaattactaa aaactataaa tgaacaaaag aaagaagttg
     2341 aggaattaaa agctgcccag gaaatcagaa ggcatgagct gactgcagta attaatgatt
     2401 taaatattaa gcaaaatgaa gtattaatag cttctcgttc aaaagaaaac tacatgtcca
     2461 gcctgcaaaa taatattgaa gaaattctaa gacaggaagc tgccatggaa gagcagtcaa
     2521 agcaaatcga aaaagacatt atagcattac agcgtgctgt tgaatatgca ggaggcgaat
     2581 tactgtggcc tctccccgga cattacagga ttacatccga atatggagga aggattcatc
     2641 cgataacagg ggtttggtca actcatggag gtacggatat tgcagctcca tacggaacga
     2701 atattctatc atcaaatgac ggggttgtaa tatatgcagg ataccattat tcttacggca
     2761 attacataat tgttgaccat gggggaggaa ttgcaacact ctatgcccat tgctcaaagt
     2821 tgttggcttc agagggacaa gcagtaacta aaggcgagcc cattgcaaag gttggctcaa
     2881 caggagaatc tacgggaaat caccttcatt acgaagtaag acttaacgga gtcaggaaaa
     2941 acccgatgga atatttaaag taatgatagt tagggacggt tcttttcaat cacacgaaaa
     3001 gagccgtcct tgatattcta aataaggagt aattatgaga attggaatag tcggttttgg
     3061 aggagtaggc agagcatttt taaatttaat ttataacaaa aaaaataaat tggaaagtga
     3121 aggaatttat tttcaggtta attatataat aggcagaacc ggtggagttt ataataagca
     3181 tggcatagat ttacataaat ttattaactt tcttttcgat gaaaaagaca taaccaagta
     3241 ttgtgacggg ggaaaagcag aagatataaa ttttgagctt atgctcaata ataaggatat
     3301 agacttaatg gttgaaatga cacctactaa taaagagacc ggagagcccg gcatgactca
     3361 tatcacaagg tgtcttgaaa acaatatcaa tgtagtgaca tctaacaaag gacccatact
     3421 gctggcttat cataagttat ataatttggc taaagctaat aaggtacaac tgggtatcgg
     3481 ttgtacaacg ggaggggcac tgccttctat taacggagga tttatcgatt tggccggagc
     3541 cgatattatt tctattgaag gagttttaaa cggaactacg aacttcattt taaaagagat
     3601 ggaggataca ggctgtaatt atgacgatgc cctgaaggaa gctcaaaggc tgggtatagc
     3661 tgaaacaaat ccggctctcg atgttgaagg atttgatact gcttcaaaac tgttaatatt
     3721 aacaaatgta ttgatgaata cggaaaaaac tatggatgat atatttatag aaggcattac
     3781 aaaattaaca ccccatgata taaatagagc aaaatccatg aacaaaaaat ataagctggt
     3841 aggtaaaaca caaattcttg ataataaaat agaaatggaa gtaaaactgc aattgctgga
     3901 cccctccaat cctttatacg gagttgaggg taagaataaa gcagtaagat atatatccga
     3961 cacattaggg gaattaacga ttatgggcgg agcttcggga gttactcctg cagcagcttc
     4021 aatattgcga gacataataa atattaacag aggttacaag tttgtaaagt agatttttag
     4081 ttggctgggg acggttcttt ttgttcttga aacaaaaaga accgtcccta ctttcacgcc
     4141 acgataatcg agtatactct gtgtttgtca tcctgagtgt aagcgaagga tatcaaatca
     4201 gagctgtttt tttggtttca tccatcaatt cgtctaattt ttctgcgttc atggtttcat
     4261 atttcaacag gtattgagca attttatcaa caactgcttc attttctcta attatcgcca
     4321 tggcgctttc atatgcttct tttgtcattt cttgagcttc atccttgata ttgtctaaat
     4381 aatatttaat aaatctttca tcaatgcatg tgttttgagt atttttgccc atgccgtaat
     4441 tgcagataat ttcataaata atatttgtgg actctttaat atcatttgaa gcacccgtcg
     4501 atatttcacc gataaatatt tcttcggaag ctctgcctgc aagaagtaca attactttgt
     4561 tgcaaagctc gtttttcgtg tagaggaatt tatcttccgt agggaatttt aaaacatatc
     4621 ccagggcttt gtctcttgga attattgaaa ttttctgaac ttggtctata ttaagaattt
     4681 tagcagcaac tgcatgacct gcctcatgat atgcaacaat tcgtttttcc ttgtctgtta
     4741 ccgtggaatg ttttatttcc aaacccgcgg caattttttc gatagcaaat tcaaaatttt
     4801 cattggtaat tttttttgat ttttccttta ttgctttaag tgctgcttca tttgctatgt
     4861 tggcaagctg agctccggaa aacccgtgag ttttaatggc aatatcctta agctgaacct
     4921 ttttgtcaag aggtttgttt ttagtgtgaa cttccaagat tttaagtctg gaatgaaaat
     4981 tagggtttcc gacataaatt tttctgtcaa atctgccggg cctcagcaag gcttcatcca
     5041 ataagtccag cctgttggtt gcagcaataa caattacatt gctggtgttg ttaaatccgt
     5101 caagctcaat taatagctgg tttagggttt gatccttttc attgttgctg tctgcatttc
     5161 ttttggcacc aagagcatcc acttcatcta taaaaattat gctgggtgct tctttgcggg
     5221 ctttttcaaa tatggttctc acccttttag cacctacacc tacatatttt tctacaaatt
     5281 ccgagccgct cgaataaatg aatgttgctt ttgcttctcc tgctattgct ttagcaataa
     5341 gtgttttgcc ggttcccgga ggtccgtata agagtatgcc tcttggaacc tttgcatcca
     5401 ttaacctgta tttatcttca ttattcaaaa aatctataat atccattaaa tcttctttaa
     5461 tctcctcaag tcctgccacg tcattaaagc ttatttgaat tttttgctca tcaatttttt
     5521 tcttatcaaa atatgttacg gtggaagcta tctcatttac ttccatatca tcatcttgtt
     5581 tcttattttt catataataa aacattgctt tagaaataaa cattgtatat aatagtaacg
     5641 gagtaaaata tagtagtggt agtttaatgt tttgaggtag tgtagcaaat aaaagaatat
     5701 atactataaa gaaaattatt aatgggaaat atttaatttt catattacct ccttatgtcg
     5761 aaaactctaa atatttttat tgatatcttt atctttacca ttacaaaaaa agttatcacg
     5821 taattctata aataattgtt gacatttatt gttatgttgt atatactgtt aaagtgttca
     5881 agaagaagta accgcttctc accttgcggc gttttatgaa ctgccggcaa ggttaaaaaa
     5941 taaatgcaat tttgtatttt tttagatggc ggtgaaaatc gctgtttttt ttatagcgct
     6001 gacccatttt tagaggtatg gggacacact ttgaatagaa atataatata atatattatt
     6061 atatataaga aacatctgaa attttaggag ggatggatta ttaaggaact tcaaatcaac
     6121 gagcaaattc gtgagaagga agtaagagtt gttgactctg atggtagcca gcttggcatt
     6181 ataagtacaa aagaggcttt aaagattgcg gaaagcaaaa agcttgactt ggtgaatgta
     6241 tcgcctaatt cagatccgcc ggtgtgcagg attatgaatt acgggaaata taaatatgct
     6301 caggcaaaaa aggaaaaaga gtctaaaaag aagcaaaaag taataagtgt caaagaaatc
     6361 agaatgacac caaatattga agagcatgac ctggcagtca aagctaagaa tgcatcaaaa
     6421 ttccttatag acggtgacag agttaaagtt gttgtgcgtt tcagaggaag agaacttgga
     6481 aagatggata ggggcagaga agtattgatg caatttgctg aaatgacatc agaatattct
     6541 acaatagata aacaccctgt tgtagaaggc aaaaatatgt ctatgttttt gaccccgaaa
     6601 aaataactaa aggaggaacc actatgccaa aaattaaaac aaacagatca gctgcaaaga
     6661 ggtttaaaaa aaccggaagc gg
//
LOCUS       DAOSOW010000052         6675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_11543_length_6675_cov_2.798792, whole genome shotgun sequence.
ACCESSION   DAOSOW010000052 DAOSOW010000000
VERSION     DAOSOW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6675
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11543_length_6675_cov_2.798792"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLD67_02330"
     CDS             complement(<1..265)
                     /locus_tag="PLD67_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21398.1"
                     /translation="MADYFARHCVSEYQSKALLDALKVSMDSYDIDVLGKLIAKWLID
                     EKNDEIEAVGGFLDDLSKLNLTSKIPVNMAEAKKPPKKQYCNIK"
     gene            594..899
                     /locus_tag="PLD67_02335"
     CDS             594..899
                     /locus_tag="PLD67_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21399.1"
                     /translation="MGYEFRMKTGFFETKTYALLVHKGELVLSPKEIDDGLITIPEES
                     ILSITIKKSHKSLEMEIQTDEKLYHGQLDNKTDYEKLINQIKESINKKILCEYEGGN"
     gene            901..5226
                     /locus_tag="PLD67_02340"
     CDS             901..5226
                     /locus_tag="PLD67_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HOT21400.1"
                     /translation="MRKKLVSWLLTVMMILSLIISVPMTAYGADEPQATNIIVANTNN
                     SSIDFFDISANGNIAPARTLKGASTNLNRPVDIALYDGELYVLNHEAHAIEIFDPLAS
                     GDTTPVRRIIGGMSYPSGFDFDPVTGEIYVANYSGNITVYNITDSGTVTPKRTIAKGN
                     TMTLAVSGNELFVTTYGESAVYVYNNVTGELLRTISGTSTGFSGNLYGVAISDGELYI
                     ADSGNNAIKVFNTTDSGNVAPKRIIAGGNTLLGTPREFHIADNKLYVANNNSVTAFNL
                     TDGGDVAPVQVLSGAATTFNAVQGVTVAEMPEIPQLIQTSETKMTDFFPKSAQGENGI
                     YLQSRDENSTYYNLDYFGDYTFKTTGTPWNLPLVMIGDKANEIIKADPSALNQVGFER
                     DSVIRTTLNGDFGWVRVTGSTQMDTKGSARFYIYKGEDGYSSPLWEATPGVTPVSFDL
                     IIPYTEGDELFFSVDAGANDIDDWAIWKNIHFAPQSGPFSGGVGTEEDPYLISTEDDL
                     FELAQKTTADNSFIGKYFIQTNDIEITTVWTPIGWRGNYYDTNLPFEGYYNGQSYSIS
                     ELNISSSISWAGLFAVIGANGTLEDINLEGVSIIAGYHSGALVGENRGLIKGCNTSGS
                     ISGAGQLGGLVGMSHNGLLEDCHSSMTVIGTQESVGGLVGYSTNNSGNAANSIVRCSS
                     TGDVTGIQRTGGLVGTLQGGLLSESFALGSVSSSNNNTGGLVGVIESANGFVAEVKNS
                     YARLSISSPNDKSVGGMFGANYWGTVSNCYAASSIAASGLNVGGLTGMIYSGTITSNS
                     YYDADLSGTSDIGKGTPKSTDEMKQISTYNNWSFPDVWTINAIDNDGYPALAWQGFPH
                     VSLSDDADLSGLSASGMTITPVFDASVTSYSANVTNNISSTTITAISASESATVKING
                     TTGNSREVPLNVGSNLFTVVVTAEDGETSKTYTININRESYVPVENQKPVLTSDWNYT
                     TVYFGQNYPFTINVTDYESTPNTLLYSYIDNAAPNQSYNFSVVPGTVNITLSPLKGVL
                     SSGSHTLNYYAVDAGGAVSSVLVLPFTIVEKTSSEASLSSISLTDINLTPAFNSGTLN
                     YTSSVSNSISSTTVTAIAVSETATVKINGVSGNSKTIPLNVGSNLITLLVTAEDGETL
                     KTYTIDITREDSGYGGGGGSSYRYYTITAKAEKGGKISPSGNISVGEGKDKEFTVTAN
                     EGYIISDVIVDGKSLGAVGNYIFKEISKAHTITARFIEEVKEELEKPESYDKEIPFTD
                     VSQNDWFANDVMWAFEKGLMNGTSSTTFDPHGITTRGMIVTILYRLEGSPSSEDNTFI
                     DVESGKYYANAVAWAAKNEIVKGYGNGKFGPEDPITREQLAVILVNYAKYKGYDVASR
                     ADLSKFTDFGEVSIWADDAMSWASFEKLIQGDGNKLMPAGNAERAQAAAIFHRFMECI
                     TSSSN"
     gene            complement(5516..6106)
                     /locus_tag="PLD67_02345"
                     /pseudo
     CDS             complement(5516..6106)
                     /locus_tag="PLD67_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005355618.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(6149..6361)
                     /locus_tag="PLD67_02350"
     CDS             complement(6149..6361)
                     /locus_tag="PLD67_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT21401.1"
                     /translation="MAISYKKLWKLLIDRDMKKKDLQKLAGISSATITKLGKNENVST
                     EIIQKICIALKCDVCDIMEMDDTKRS"
BASE COUNT         2129 a         1243 c         1271 g         2032 t
ORIGIN      
        1 tctttatatt gcaatactgc ttcttcggcg gcttttttgc ttctgccata tttaccggta
       61 tcttcgaagt taaatttaac ttactcaagt catcaagaaa acctccaacg gcttctattt
      121 catcattttt ttcatctatg agccattttg ctatcaattt gccaagaacg tctatgtcat
      181 agctgtccat acttactttc aatgcatcca acaaagcttt gctctgatat tcagatacac
      241 aatgccgtgc aaaataatca gccattttag ttatgaccgc ctcatccttt atggctttgc
      301 gttgtcccct tctgagtctg ctgatgtgag acggatcaat ctttaccttc tgccctaaag
      361 cactgttact tgtatttgtt atattcatta gaaaatctaa tttttcagaa aattgcaatc
      421 ttatcacctc tgcttgatta tacgtccacg gtaatatata atcaatgtta tttgacatga
      481 ttcatgtcaa aaaatttgac acggtcagtt ttttaggagc tcttccataa taagatttat
      541 tgaagtataa ttcttccaag tagtctaaaa ccaattgaaa cgaggtggtt aggttgggtt
      601 atgaatttcg tatgaaaaca ggtttctttg aaactaaaac atacgctttg cttgttcata
      661 aaggagaact ggttctttct cccaaagaaa ttgacgacgg attaataaca ataccggaag
      721 agagtatttt gagcatcaca ataaaaaaaa gtcataaatc tttagaaatg gaaatccaaa
      781 cagatgagaa gctttatcat ggtcaattag acaataagac ggactacgaa aaactcatta
      841 atcaaattaa agaaagtatt aacaaaaaaa tcttatgtga atatgaagga ggaaattaaa
      901 atgcgtaaga aattagtttc atggttattg acagtaatga tgatattatc gcttatcata
      961 tctgtaccaa tgactgctta cggtgctgat gaacctcaag caaccaacat tattgtcgca
     1021 aacaccaata acagcagtat tgattttttt gatatttcag ctaatggcaa tattgcacca
     1081 gccagaacac tcaagggagc aagcactaac ttaaaccgtc cggttgacat tgctctatat
     1141 gatggggagc tatatgtatt aaatcacgaa gcacatgcta tagaaatatt tgatccgctg
     1201 gcttcaggcg atactacacc tgtaagaaga atcattggcg gtatgtctta tccttccggc
     1261 tttgatttcg acccggtcac aggtgagata tatgtagcca attatagcgg taatattacg
     1321 gtttataaca ttacagacag cggcaccgtc acacccaagc gcacaatcgc aaaaggcaac
     1381 accatgacac tggctgtgtc cggaaatgag ctgtttgtaa ctacatatgg agaatctgca
     1441 gtatacgttt ataacaatgt caccggtgaa cttctccgta ccatatcagg tactagtact
     1501 ggattttcag gaaatctata tggggtcgct atatccgatg gggaattata cattgccgac
     1561 tccggcaaca atgcaataaa ggtgttcaac acgaccgaca gcggcaacgt tgcgcccaaa
     1621 cggataattg ctggtggcaa cacccttctg ggcacgccgc gagagtttca cattgccgat
     1681 aacaagcttt atgttgcaaa taataactca gtcaccgcct ttaatttgac agacggcgga
     1741 gatgttgcac cggttcaagt cctgtctggc gcagctacta cctttaacgc cgttcaggga
     1801 gtcacagtgg ctgaaatgcc ggagattcca caacttattc aaacaagtga aacaaaaatg
     1861 accgactttt ttccaaaatc tgctcaggga gaaaacggaa tttatctgca atcacgggat
     1921 gaaaacagta cttattataa tcttgattac ttcggagact atacgttcaa aacaactgga
     1981 acaccttgga atcttcctct tgtaatgatt ggcgataaag caaatgaaat cattaaagct
     2041 gatccttcag ctttaaatca ggtcggattt gaaagagaca gtgtcatccg cactactcta
     2101 aacggtgatt tcggctgggt gagggtaaca ggcagcactc aaatggacac caaaggatct
     2161 gccagatttt atatctacaa aggtgaagac ggttattctt ctcctttatg ggaagcaact
     2221 cccggagtaa cgcctgtaag ttttgacttg ataattccgt atactgaagg tgatgaattg
     2281 ttcttttctg tggacgccgg tgccaatgac attgatgact gggctatatg gaagaatatc
     2341 cactttgccc cgcagagtgg accattctca ggtggtgtag gaacagaaga agatccctac
     2401 ttaatttcaa cagaggatga tttatttgaa ttagctcaaa aaactacagc tgataactcc
     2461 tttataggta aatatttcat tcagacaaat gatattgaga taacaacagt atggactcca
     2521 ataggatgga gaggcaatta ctatgatact aatctgccat tcgaaggtta ttataatgga
     2581 caaagctaca gtatttctga gttaaatatt tcctcttcca taagctgggc aggacttttc
     2641 gcagttatag gtgcaaatgg aacattagaa gatattaatc tggagggtgt cagcataata
     2701 gcaggatatc attctggtgc tttagttgga gaaaacagag gcctaattaa aggatgtaac
     2761 acatctgggt caatcagtgg agccgggcag ttgggtggac tggttggcat gagtcacaat
     2821 ggtttattag aagactgcca ttcctcaatg actgtaattg gaacacaaga gtctgttggt
     2881 ggtttggtgg gttactcaac aaataattcc ggaaatgcag ctaattcaat tgtaaggtgc
     2941 agttccaccg gtgatgttac cggtattcag cgtacgggag gacttgttgg tacattgcaa
     3001 ggtggattat tatcggaaag ttttgctctt ggtagtgtaa gcagttcaaa taataatact
     3061 ggtggactcg tgggagttat tgagtctgcc aatggttttg ttgctgaagt taaaaatagc
     3121 tacgcaagat tatctatctc atctccaaat gataaatctg taggaggaat gtttggagca
     3181 aattactggg gtactgtttc taattgttat gcagcaagta gtattgcagc atccggttta
     3241 aatgtgggtg gtctaacagg tatgatttat tcaggtacaa ttacatcaaa cagttattat
     3301 gatgcagatt tatctggaac gtctgatatt ggtaagggaa caccaaaatc aactgatgaa
     3361 atgaaacaaa tttcaaccta taataactgg tcattccccg atgtatggac tattaacgca
     3421 attgataatg atggttatcc ggctcttgca tggcagggtt tccctcatgt atcactttcc
     3481 gatgatgcag atttatcagg tctgtcagct tcaggcatga caataacacc ggtttttgat
     3541 gcttctgtaa caagctattc agctaatgta acaaacaaca ttagcagtac tacaattaca
     3601 gcaatttctg cttcagaatc tgcaacagtg aagattaacg gtactactgg caatagcaga
     3661 gaagtaccac tgaatgtagg aagcaattta tttacagtag tggttactgc tgaagacggt
     3721 gaaacttcta agacatatac aattaacatt aatcgagaaa gctatgttcc tgttgaaaat
     3781 cagaaaccag ttttaacttc tgactggaat tacaccacag tttattttgg ccagaactat
     3841 ccttttacta taaatgttac agattatgaa agtacaccaa atacgctgtt gtatagctat
     3901 attgataatg ctgcaccaaa tcagtcatat aatttttctg tagtaccggg tacagtcaac
     3961 ataaccctat caccattaaa gggagtttta tcttctggta gtcatactct aaactattat
     4021 gcagttgatg caggtggtgc tgtatcatcg gtgttggttc taccttttac gattgtagaa
     4081 aaaacgtcaa gtgaagcctc attgtcaagc atttcattaa cagacataaa cttaacaccg
     4141 gcttttaact cagggacttt gaattatact tcaagtgtca gcaacagcat tagcagcact
     4201 acagtcacag caattgctgt ttcagaaact gcaacagtaa aaataaacgg tgtttctgga
     4261 aatagcaaaa caataccgct gaatgtaggc agcaatttaa taactctatt agtcactgct
     4321 gaagacggcg aaactttaaa aacttataca attgatatta caagagaaga cagcggttat
     4381 ggtggaggcg gtggcagttc atacagatac tacaccatta cagctaaagc agaaaaaggt
     4441 ggaaaaattt caccatccgg aaatatttct gttggtgaag gtaaagataa agaatttaca
     4501 gtaacagcaa atgaaggtta tataatttct gatgtaatag tagacggaaa aagcctaggt
     4561 gctgtaggca actatatatt taaagaaata tccaaagctc atacaatcac agctaggttt
     4621 attgaggaag ttaaggaaga attagaaaaa ccggaaagct atgataaaga aattcctttt
     4681 acagatgttt cacaaaatga ctggttcgca aatgatgtta tgtgggcatt tgaaaaaggg
     4741 ctgatgaatg gaacaagcag taccactttc gatcctcacg gaattactac aagaggtatg
     4801 attgtaacca ttctttatag attagaaggg tcaccgtcat ctgaagataa tacattcatc
     4861 gatgtggaat caggaaaata ttatgcaaat gctgtggctt gggcagcaaa gaatgaaatc
     4921 gtgaaaggct atggcaacgg aaaatttggt cccgaagacc caattaccag ggagcagctg
     4981 gctgtaattt tagtgaatta tgctaaatat aaaggttatg acgtagcatc aagagctgat
     5041 ttatccaaat ttacggattt tggggaagtt tcaatttggg cagatgatgc tatgtcttgg
     5101 gcaagttttg aaaagctgat tcagggcgat ggcaacaaac taatgccggc aggcaatgca
     5161 gagcgagctc aggcagcagc aatttttcat cgttttatgg agtgtatcac aagtagcagt
     5221 aattagaaca atgttttata aacctggtaa tataggttgt gaaaattgtg aaggcaaggc
     5281 aatatgggta ttgtcaatat ttctgattat gctataggca ctgcaattca taatgagtaa
     5341 tcaaaataag tgaatcaaaa atgcctgact cttttttcgg ctaggcattt ttgtgcttat
     5401 ttaccctgct attttctcga acaaataaaa tatgcacacc cctttttgag tttgcacacc
     5461 cctaaacata acacttttta ggataattaa attgtatcaa tttctgcact attatatcca
     5521 acactatccc atgagtcggc tcaattacat tcacaatcat ctttaccaaa gatttaggtg
     5581 taaagaaaac tccatcatcc gatgcgatag ctgaagcaaa cttatttaag aaatactcat
     5641 aaatacgtcc aaggatatca tcgttgattt cgttcaatgc actattatta aaaatacgca
     5701 acaattcttt caacaaatca tttctgaaag aagtatatgt tttgggtagt attcctttca
     5761 attgagtact ctctgcctca ataagctcca tagcttcatt gactgctttc ccacaatcag
     5821 catcgtctgg caaattgagc agataatcat atcgagcctt ttcaggtaaa aaaatagcgc
     5881 ttttttgctt aaaatcactt gactcaacgg gaagtacacg accattacgt acaggacggt
     5941 cttttagtat ttctgcctct acatatttaa accggctata tgcataacgc aaaaagatta
     6001 aaccaagaac cggcatgcaa tattcttgag aacttaattt agaaccttga cgtaagagat
     6061 ccgcagattc ccataattca gattctaatt ttcttatatt tatcattaaa caagcctcca
     6121 tttgtttgat atttaacaat aataagtatt aactccgttt ggtatcatcc atttccatga
     6181 tatcgcagac atcacatttc agagcaatgc atattttttg tattatttct gtactaacat
     6241 tttcgttttt accaagcttt gttatcgtag cagaactaat acccgcaagc ttctgcaagt
     6301 ctttcttttt catatctcta tcaataagaa gcttccatag ttttttataa ctaattgcca
     6361 ttctaatcac ctctatgtat gtataattac atgatatcat tagcatcatc atattgcaaa
     6421 cattttcttt atgttcgcat gatttttgat ttgtattcaa taattcttac tccaaatcct
     6481 tctaaattct atgctataat ctcaggtgta tagaatgtta catccatcct gtctcctcaa
     6541 ccctgcttat ttatcaattc tgtcaactca acccaaccta atttcagact catttttagc
     6601 aaaaaaacac cctcgatatg tttccacata cacatatcga gggcttaggt cgagtggata
     6661 gattcacttt cattt
//
LOCUS       DAOSOW010000053         6674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_11546_length_6674_cov_3.146699, whole genome shotgun sequence.
ACCESSION   DAOSOW010000053 DAOSOW010000000
VERSION     DAOSOW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6674
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11546_length_6674_cov_3.146699"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /locus_tag="PLD67_02355"
     CDS             complement(<1..1024)
                     /locus_tag="PLD67_02355"
                     /inference="COORDINATES: protein motif:HMM:NF028468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HOT21402.1"
                     /translation="MTDIKKKNNIYIEIILIIISSLLLFITSAYYNDVFTEIMSVATY
                     LTWHNLFEFLSIIASFSIFIVTYYIYEESGNLRMIILACSFFTMAFIDTFHTLSFKGM
                     ADFFVANLTANRATTFWILSRLLGSTGFMISSFIPEYKRSELNKWVFIIPTSILVISL
                     LIVVTYIPDFIPAMYVENYGLTETKIILEYLVVIIMIIAFLKIYREYKRNNKRLDYLI
                     MIALLLSIFSELAFISYGSVYDAYNYLGHIYKVIAYFFLFKAIYAESVSIPYRELKKA
                     KNELKDYADNLNLLVKERTKELENMNEILMNDIESARLMQRSLLPEKLPESQTVTFYA
                     AYIPAER"
     gene            complement(1168..1788)
                     /locus_tag="PLD67_02360"
     CDS             complement(1168..1788)
                     /locus_tag="PLD67_02360"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOT21403.1"
                     /translation="MKKVLTIVLVFALSFGFLTIVNNMGNTDTASEQQTLTGTAEGFG
                     GDINVTVVVNGEDIISVEVTADGETQGIGSMAIDELPALIVEADSAEVEGVSGATYTS
                     DGIKAAVRNALSSKQGSEEEVTLTGTAEGFGGDVTVNVVVKGDDIISVEAIGEDETEG
                     IGSVALVELPALIAEADSTDIDGVSGATYTSDGIKAAVRNALESRQ"
     gene            complement(2004..2690)
                     /locus_tag="PLD67_02365"
     CDS             complement(2004..2690)
                     /locus_tag="PLD67_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOT21404.1"
                     /translation="MKTLYEGKTKKVLKDEKDNSVYIFFKDSATGENGVFDPGSNTVG
                     GIVEGKGKIGLIISKYFFELMEKNGIPTHYLGADIENGLMKVRNLTVPKLEFVLRYFT
                     AGSMCRRFSLDEGIPFDPPYAEVTLKDDEQGDPLISERLCIMKGLLSEGQYDEALRLL
                     EKVGEVLKKELAQMALTLIDFKIEIGYDESGKMYVVDEITPDIWRVRDKNGNVPNQIE
                     CSKLILNKIN"
     gene            complement(2717..3166)
                     /gene="smpB"
                     /locus_tag="PLD67_02370"
     CDS             complement(2717..3166)
                     /gene="smpB"
                     /locus_tag="PLD67_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOT21405.1"
                     /translation="MADKIVANNKKAFHEYFIEDKYEAGLVLVGTEVKSIRQGKVNLK
                     DSYVSIKNGEAFVYNMHISPYEKGNIYNVDPLRPRKLLLNKRELRKLAGLTTLKGYSL
                     IPLSLYLKNGLVKMELSVAKGKKTYDKRQDIAKKDAERRMQRQDDYR"
     gene            complement(3249..3950)
                     /gene="pyrF"
                     /locus_tag="PLD67_02375"
     CDS             complement(3249..3950)
                     /gene="pyrF"
                     /locus_tag="PLD67_02375"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006058830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOT21406.1"
                     /translation="MGKDVIIACDFSNREEVVNFLSKFTEEKPYVKVGMELFYAEGPE
                     ILKLIKAMGHKIFLDLKLHDIPNTVRKAMKVLSEYNVDMVNIHAAGTISMMEAAIEGL
                     TRKDGSRPILVAVTQLTSTDEERMKHEILIDKPMEEVIMHYAKCAKKAGLDGVVCSPL
                     EAGKVHDICGKDFLTVTPGVRFADNDSGDQVRITTPAKAKELGSDYIVVGRPVTQAKD
                     PVEAYRRFVKEFIEG"
     gene            complement(4371..5819)
                     /locus_tag="PLD67_02380"
                     /pseudo
     CDS             complement(4371..5819)
                     /locus_tag="PLD67_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006907390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
     gene            complement(5816..6376)
                     /locus_tag="PLD67_02385"
     CDS             complement(5816..6376)
                     /locus_tag="PLD67_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOT21407.1"
                     /translation="MGQGTVQSNKLLDILGLNEAQKEILMIATSEESCSELQDKLSEH
                     FKFSKKNKGIAFSIPFTRWKLKSNEEEKREIHREFNPSHYCIMTIVDKGRSKDCIKAA
                     KAAGARGGTIMHGHGAGVPIDYYFPLIIEPQKDIVMILTPKDNVTAIMDKIIADLELY
                     KEGKGVIFALPVVKISGLIENKGVRV"
BASE COUNT         2066 a         1365 c          955 g         2288 t
ORIGIN      
        1 gtctttcagc aggtatatag gctgcataaa acgtaactgt ttggctttca ggcagttttt
       61 caggaagaag cgagcgctgc attaaccttg cactttctat gtcattcatt aatatttcat
      121 tcatattctc aagctctttc gttctttctt taacaaggag attaagattg tctgcataat
      181 cctttagttc atttttagct ttttttaatt ctctatatgg aattgacacg ctttcagcat
      241 aaatagcctt gaataggaaa aaatatgcta ttactttgta tatatggcca agatagttat
      301 atgcgtcata tacgcttcca tagcttataa aagcaagctc actgaatata gacaatagaa
      361 gtgctatcat aataagatag tctaaccttt tattatttct tttgtactcc cgataaatct
      421 ttaaaaaagc aataatcatt attataacaa ctagatactc caaaattatt ttggtttctg
      481 taagtccgta attctcaaca tacatggcag gaataaaatc cggtatatat gttacaacaa
      541 ttaacaagct aatgactaat atagatgttg gaattataaa aacccattta tttagctcac
      601 ttcttttata ttcaggaatg aatgaagaaa tcataaagcc tgtacttcct aataatcttg
      661 aaagtatcca aaaggtagta gctctgttgg cagttaaatt tgccacgaaa aaatctgcca
      721 ttcctttaaa gcttaatgta tgaaatgtat caatgaaagc cattgtaaaa aaagagcagg
      781 caagtattat catacgaaga ttgccggatt cttcatagat gtaatatgta actataaata
      841 ttgaaaacga tgcaattata ctcaaaaatt caaataaatt atgccacgtt aaataagtcg
      901 ctaccgacat aatctcagta aatacatcgt tataatatgc tgaagttata aataacagaa
      961 gtgaagatat gataataagg ataatttcta tatatatatt attcttcttc ttaatatccg
     1021 tcatctcctt tcaatacagc taatggagat ttttgtagat ttttgtagat attttaatta
     1081 tagtatacaa tcaatattta taccagtatt ttttaatata aaaaagctat accaagatag
     1141 tatagcttct tacccgattc ttaaggacta ttgcctggat tctaaagcat tccttacggc
     1201 agcttttata ccgtcacttg tataagtagc acctgatact ccgtcaatgt ctgttgaatc
     1261 tgcttctgct attaaggcag gaagttctac aagtgcaaca cttcctatac cttctgtttc
     1321 gtcttcgcct attgcttcaa cagatattat atcatcgcct tttacaacca cgtttacagt
     1381 aacatctccg ccaaaacctt cagctgtacc tgttaatgtt acttcttctt cagaaccttg
     1441 cttagatgat aaggcatttc taacagcagc ttttataccg tcgcttgtat aagtagctcc
     1501 cgatactcct tcaacctctg ccgaatccgc ttctactatc aaagcaggaa gctcatcaat
     1561 tgccatagac cctattccct gtgtttcacc gtcagctgtt acttctactg aaattatatc
     1621 ttcaccgttg acaactaccg taacatttat gtcaccgcca aaaccttcgg ctgttcctgt
     1681 tagtgtttgt tgttctgatg cagtatctgt attacccata ttgttaacta ttgttaaaaa
     1741 tccaaaagac aatgcaaata ctaagacgat cgttaatact tttttcatta tttcctccaa
     1801 ttactataca tcgataaata tttatttttt cataacatta aaaattatac aacaattgta
     1861 tggccgtgta aagaagaata atattcctaa atacatgtaa tttataccca taaaaatttt
     1921 tatgaaacat atatcttgat tttctttata ttaaaaaccg cttatttgat aagcggtctt
     1981 atttatcatt taaaaaatct ctattaattt attttgttta aaataagttt gctgcattca
     2041 atttgatttg gtacattacc attcttgtca cggacacgcc agatatcggg tgttatttcg
     2101 tccactacgt acattttgcc gctttcatcg tatcctattt ctattttaaa gtcaattaat
     2161 gtaagagcca tctgtgccaa ctctttctta agaacttctc caaccttttc taataatctt
     2221 aatgcttcat catattgtcc ttctgataaa agacctttca taatacacaa tctttcgctg
     2281 attaaggggt ctccctgctc atcgtctttc aatgtaacct cagcataagg aggatcaaag
     2341 ggaattcctt catctaagct gaatcttcgg cacatactgc cggcagtaaa atagcggagt
     2401 acaaattcca actttggtac agtaagattt cttactttca tcagcccatt ttctatatcc
     2461 gctccaaggt aatgggtagg tatgccgttt ttctccatga gctcaaaaaa atacttggaa
     2521 attataaggc cgatttttcc cttgccttca acgattccac caacagtatt ggagccgggg
     2581 tcaaaaactc cgttttctcc ggttgcgctg tctttaaaaa agatatatac cgaattatcc
     2641 ttctcatctt ttaaaacttt ctttgttttt ccctcataca gtgttttcat tgctgttctc
     2701 ctcttcaaag taaaatttat ctgtaatcat cttgtctttg cattcttctt tcggcatctt
     2761 ttttggcaat atcttgacgt ttgtcataag tctttttacc ttttgctacc gagagttcca
     2821 ttttaacaag accatttttc aaatataaac ttaaaggtat aagtgaatag ccttttaaag
     2881 ttgttagtcc tgcaagtttt cttaattccc ttttgtttaa aagcagtttt ctcggtctta
     2941 aggggtctac attatatatg tttccctttt cataaggact tatgtgcata ttataaacaa
     3001 atgcttcacc attttttata cttacatagc tgtccttcaa attgaccttc ccctgtctta
     3061 ttgatttaac ttcagttcct acaaggacta atcctgcttc atatttatct tctataaaat
     3121 attcatgaaa tgccttttta ttgttggcta caattttatc cgccattcta ccacctaccg
     3181 ttaacgaaaa tgattatagc acttaataat tatagtgtca aaccattaat ttccacatta
     3241 actgataatt atccttcaat aaactctttt acaaaccttc tgtatgcttc tacagggtcc
     3301 ttagcttgtg tgacaggtct tcctacaact atataatcag agcctaattc ttttgccttg
     3361 gcgggagtcg taattctgac ctggtctcct gaatcattat ctgcaaacct tactcccgga
     3421 gttactgtaa gaaagtcttt tccgcatata tcatgtactt ttcctgcttc aagaggagag
     3481 cagacaaccc cgtccaagcc cgccttttta gcgcattttg catagtgcat tataacttct
     3541 tccatgggct tatctattaa tatctcatgt ttcattcttt cttcatcggt tgaagtaagc
     3601 tgagttactg caactaatat cggtcttgaa ccatccttgc gggttaaccc ttcaatagct
     3661 gcctccatca ttgaaatggt tcctgctgca tgaatgttaa ccatatcaac attgtattcc
     3721 gacaaaactt tcatggcttt tctaactgta ttgggaatat catggagctt aaggtctaaa
     3781 aatattttat gccccatggc tttaattagt tttaaaattt caggcccttc tgcgtagaat
     3841 aattccatac ccacctttac atagggcttt tcttcagtaa attttgaaag aaaatttaca
     3901 acttcttctc tattgctgaa atcacaagca ataattacat ccttgcccat atcgtctcct
     3961 tattcatatt aattttccca ttttttgttt tattaatact taatcattgc atctgcgacg
     4021 ggacatatat agataacaat atccccttct tctttaagtt ttttatcgct taataaattg
     4081 tcagcaactt tctcagtgga atttgatttt gtaataaata taataatttc tttttcttgc
     4141 tgcaactcta catttataat aggcaacata actttatgat gtatgtctgt ccctcttcca
     4201 tgcataatgg tagctccgct tgcacctgac tgacgcgcta ggtctactac attttcagca
     4261 aagcctcttt ctacatttac cattatgcag tcgtagagca atctgttttc ttcaggtata
     4321 gacggagtaa taacaaattc ttcctcagtc ggagtatatt cttcaacttt cttataccta
     4381 aaaacggttc ccaacaacat gattgaaaat acgggagcca ttgctaccat cgcaataacg
     4441 ccaaaaccat ccactaagac attagctgtc tctatagaag atgcagctcc ttgagcaaag
     4501 gcaagaacaa aggttgcagt catgggacct gaagcaactc ctcccgcatc atatgcaatt
     4561 cccacaaaga caggctctgc aataaatgat agcaaaatgg caaatgcaaa tccgggaaaa
     4621 agaaaatacc ataactttac ctcaggaatt aaaattcgta ccattgatag agaaattgct
     4681 aatgctacac ccattgaaag tgtcatcctt attaaattta atggtatatg accaccggta
     4741 acttcttcaa tttgctcgcc caagacgtga acggcaggtt caacaagaac aactataaag
     4801 cctaccacaa aaccaactcc aaccaatatc catggattca ttttagcaat ttccatgcct
     4861 aatatcctcc ccatatccat aaatccagag ttaaccgcgg taaggaataa taccaaacca
     4921 agcagtacta acacaagtcc tcttattatt ccaaagagct catccttgga taatttaaat
     4981 ttcttaaaat tataaatgaa aaataaaatt gctatgggta agagtgctac taatgattca
     5041 ataaatattg caggtaatat atttataatg ggtcctaata caccttttga cgctacaaat
     5101 tcagcagctt caccttgtat gtttttctga cccgtaatta ttgatataag cataattgcc
     5161 aaaatgggtc ctgcactcat gataccaacc agtccgaaag aattctcttc agattttttg
     5221 ccgcctttta cttgagacaa tcctaaggac aatgccaata caaaaggtgt tgtaagagcg
     5281 ccggtagtcg caccggaagc gtcaaaagaa atagcaagaa attcctcaga aacaaacaat
     5341 gccaatacaa aaattacagt ataggttatt gccataaata tattaaggga catatctttt
     5401 aataatctat aaactccaag tgaaattaat actcctacac caattgatac tatgtaaact
     5461 gtaaatgtag cattaagact tcctccggag gcctcctgaa tctgggaccc taatatcaat
     5521 aaatcaggtt ctgcaacagt aattaaaaat cctaaaagaa aactaagaat tgcaatttta
     5581 agaaatgatt ttgaagtagc tacttcccga gacatatgct ctccaattga attcatggat
     5641 aaatctactc cccacagaaa gattgaaagc cctattaaga gcataacact tccaactata
     5701 aagcgtatta aaacatcatt tccaacatct actatggtaa aacaaagcag caatacaagt
     5761 ataacaaccg gaagcaaggt ttgagtaact tctttggttt tttcaaaaag taaattcata
     5821 cccttacccc cttattttca ataagtccgc ttattttgac aaccggaaga gcaaaaataa
     5881 caccctttcc ttctttataa agttctaaat ccgctattat cttgtccatg attgcagtaa
     5941 cattatcttt aggggttaaa atcatcacta tatccttttg aggttcaata atcagcggga
     6001 aataatagtc tattggtact cctgctccat gtccgtgcat tatggttccg cctcttgctc
     6061 ctgcagcttt tgcggccttt atgcagtctt tgcttctgcc tttatctacg attgtcatta
     6121 tacagtagtg tgagggattg aattctctat gtatttctct tttttcctct tcattcgatt
     6181 ttaatttcca acgagtaaag gggatagaga atgcaattcc tttatttttt ttagaaaact
     6241 taaaatgctc gctaagttta tcctgtaatt cgctgcatga ttcttctgaa gttgcaatca
     6301 taaggatttc tttttgagct tcattaagac caagtatatc caataatttg tttgattgca
     6361 cagtaccttg acccaaaaag attgttcctc ccgttgcgcc gcattcctgt gctttgctca
     6421 gcacctcgtt ggccttgcct ctgtttacaa cagtaaataa taacgtattt atatcatcac
     6481 ttcctttata ttattatcta tgtaagatga ctacataatt atagcacatt taagaagcta
     6541 taatcaacag aaataggata caacttcatt tgacttcccc aattattttg gaatgaatca
     6601 atcacaaaaa aactccccta tttgaagagt ttttaacatt ttaattactt tgatggaggc
     6661 ggtgggagtc gaac
//
LOCUS       DAOSOW010000054         6627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_11674_length_6627_cov_3.526172, whole genome shotgun sequence.
ACCESSION   DAOSOW010000054 DAOSOW010000000
VERSION     DAOSOW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11674_length_6627_cov_3.526172"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /gene="flgN"
                     /locus_tag="PLD67_02390"
     CDS             complement(<1..265)
                     /gene="flgN"
                     /locus_tag="PLD67_02390"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HOT21408.1"
                     /translation="MDSLYQLLKEYTEIYKMFLELEYDKYEAVIEDDVIALDNIVSQE
                     EVYYLQMKGIEHKREKLLNSLELSGKTLKEIIDAAENESKIKLQ"
     gene            complement(275..553)
                     /locus_tag="PLD67_02395"
     CDS             complement(275..553)
                     /locus_tag="PLD67_02395"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOT21409.1"
                     /translation="MKINNINNNNYINYKGNSNLIKKEEINKENKYDVIDFNNKNKEN
                     ISLQSIKKKVVSQINEETNADKINRIKDSINNKTYSIDVEEIVRRLLR"
     gene            complement(624..1103)
                     /locus_tag="PLD67_02400"
     CDS             complement(624..1103)
                     /locus_tag="PLD67_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOT21410.1"
                     /translation="MNNENIMQMREVIPWLIFKLNETLYTVNSKYITSIVIKPDDVTF
                     VPNVAEHIIGLIHLRGNVIPLVDLKLLLNTEAESGKNSANNSKENKAMVVVLEKGNSF
                     IGLVVDEVLSVENIFAYEETEEIKQMCNNGFITGVAKGHKSEEVLLVIDEEKIMSNA"
     gene            complement(1096..1620)
                     /locus_tag="PLD67_02405"
     CDS             complement(1096..1620)
                     /locus_tag="PLD67_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009062629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOT21411.1"
                     /translation="MDKFNSLHTYIEEEDAQVNKYLTFYIENQVFGIPIAHVVQITGM
                     QEIEEVPEFPSYAKGIINLRGIIVPIIDVRIRLKKQEIEYNERTCIIVTNIEGLYIGF
                     IVDSVNEVINIYYENISEPPQVGNNYVITYITGIAKLDKKIVILIDLNKLLNEKEIGS
                     LELIKDEIMGDYNE"
     gene            complement(1773..3398)
                     /gene="groL"
                     /locus_tag="PLD67_02410"
     CDS             complement(1773..3398)
                     /gene="groL"
                     /locus_tag="PLD67_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOT21412.1"
                     /translation="MAKIIKYDEDARRAMERGVNALADTVKVTLGPKGRNVVLNKSFG
                     SPQITNDGVTIAREIELSDPYENMGAQLVKEVATKTNDVAGDGTTTATLLAQAIIREG
                     LKNVAAGANPMILKKGIEKAVAAAVEELKNITKKVETSEEIAQIASISAGDEEIGKLI
                     SQAMDQVGKDGVITVEESKTMDTELVVVEGMQFDRGYLSAYMVTNTEKMEAVLEDPYI
                     LITDKKITNIQDVLPVLEQIVQTGKSLLIIAEDVEGEALATLVVNKLRGTFNCVAVKA
                     PGFGDRRKEMLRDIAILTGGQVISEELGLELKETTLDMLGNAGTVKIDKENTVIVNGA
                     GSKEEIEERARQIRAQHDESTSEFDREKLMERLAKLTGGVAVINVGAATETEMKEKKL
                     RIEDALNATRAAVEEGLVSGGGVALLTTIDKVNKVAEGLDGDAKTGAKIIEKALEEPL
                     RQIAINAGLEGSVIVEKIKNSEKGVGFDALNERYVNMIEAGIVDPTKVVRSALQNAAS
                     VAAMLLTTEAAVTDEPKKEEPAMPPMGGGMGMM"
     gene            complement(3414..3695)
                     /locus_tag="PLD67_02415"
     CDS             complement(3414..3695)
                     /locus_tag="PLD67_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOT21413.1"
                     /translation="MTVKPLGERVVIKMIEAEEKTKSGIVLPGSAKEKPQVAEVLAVG
                     AGITNDEKKKDEIKVGDKVIYSKFAGTEVKIDGEELIIIKLADVLAVIE"
     gene            4050..4979
                     /gene="fba"
                     /locus_tag="PLD67_02420"
     CDS             4050..4979
                     /gene="fba"
                     /locus_tag="PLD67_02420"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOT21414.1"
                     /translation="MALVTSQEMFKKAYEGKYAVGAFNVNNMEIIQGIVEAAKQENAP
                     LILQVSAGARKYANPIYLRKLVEAAIEDTGLDICLHLDHGEDFEICKQCVDDGFTSVM
                     IDGSKYPFEENIALTKRVVEYAHSKGVVVEAELGKLAGVEDAVKVRTKDATYTDPDEA
                     VEFVERTGVDSLAIAIGTSHGAYKFKGDPTLDFERLETISNLLPGFPLVLHGSSSVPK
                     EFVDLCNKYGGQIPGAAGVPEEMLRRAAQLGVCKINIDTDLRLALTACIRQFFVEHPS
                     EFDPRKYLGPGRTAIKDMVAHKMRNVLGCSNKR"
     gene            complement(5044..6234)
                     /locus_tag="PLD67_02425"
     CDS             complement(5044..6234)
                     /locus_tag="PLD67_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOT21415.1"
                     /translation="MTDFIIKKFIKDYENVNEQRVRESYGITASITGIILNTLLSIGK
                     ILTGIIFNSISVTADGVNNLSDGASSVITLIGFKISSKPADKDHPFGHARMEYLTGLI
                     LGIAVILVGVELIKSSFDKIMNPTKTIFSIEMIVVLIVSVLIKLWISLFYKKLGDKIS
                     SATLKASSTDSRNDVISTIVVILSLLVSKATGYEVDGYVGIIVALFILYSGYDILRDI
                     LNPLLGEMPDKEFIESIENKILSYDGIVNIHDLVVHNYGPNRYFASVHAEVDAKEDIM
                     KSHDLIDNIERDFAREFDISLVIHLDPVITDDEEINGLRSMTDKIVKSIDERLTMHDF
                     RVVKGETHTNLIFDVVVPVDYDIKSSKLVSMIEKEIQNKDETYFAVVTVDKNYVSTYM
                     NDLK"
BASE COUNT         1858 a         1295 c          976 g         2498 t
ORIGIN      
        1 cttgtaattt tattttagat tcattttccg ccgcatcaat tatttctttc aaagtcttgc
       61 cgcttaattc caatgaattt agaagttttt ctctcttatg ttcaattcct ttcatttgca
      121 gataatatac ttcttcttgt gacactatat tatctaatgc aattacatca tcttctatta
      181 ctgcttcata tttatcatat tctagttcca aaaacatttt gtaaatttcg gtgtattctt
      241 ttaataattg gtataaagaa tccataatag ccccctacct taataacctt cttactattt
      301 cttcaacatc tatgctataa gttttattgt ttatgctgtc cttaattctg ttaattttat
      361 cagcatttgt ttcttcgtta atttgtgata ctactttctt cttaatagat tgaaggctga
      421 tattttcctt atttttatta ttaaaatcta ttacatcata tttattttct ttattgattt
      481 cttctttttt gattagatta gaattaccct tatagtttat ataattatta ttgttgatat
      541 tattaatttt cataacgtcc ctcttctatt tattttcata taatattaat cgacagaaat
      601 acaaaaaaat ttagctaatt tcatcatgca ttgctcatta ttttttcttc atctataact
      661 aaaagaacct cttcactttt atgtcctttg gcaacacccg ttataaagcc gttattacac
      721 atttgtttaa tttcttcggt ctcttcataa gcaaaaatat tctctacaga taaaacttca
      781 tctacaacga ggcctataaa cgaattgccc ttttccaaga ctacaaccat agctttgttt
      841 tcttttgaat tattggccga attttttccg gattcagctt cggtattcaa taataatttt
      901 aagtctacca agggaattac gtttcctctc agatgtatca atccgataat atgctccgct
      961 acattaggca caaatgtaac atcatccggt ttgataacaa tcgatgttat atacttactg
     1021 tttacggtgt ataaggtttc attaagctta aatatcagcc agggaatgac ctctcgcatt
     1081 tgcataatat tttcattatt cattgtaatc tcccattatt tcatctttaa ttaattccaa
     1141 ggatccaatt tctttttcgt taagtaactt attaaggtca attaatataa ctattttttt
     1201 atctaatttg gcaattcccg ttatataagt aataacataa ttattgccca cctgcggcgg
     1261 ctcggatata ttttcataat atatatttat aacttcattg acggaatcaa caataaagcc
     1321 gatgtataag ccttcgatgt ttgtaacaat tatacatgtc ctttcattgt attcaatttc
     1381 ctgttttttt agccttatcc tgacatcaat tatgggaacg ataatccctc ttaaattgat
     1441 aattcctttt gcatagcttg gaaattcagg gacttcttca atctcttgca tccccgttat
     1501 ttgtacaaca tgagcaatgg gaattccgaa tacttgattt tcaatataaa aagtaagata
     1561 tttattaact tgagcatctt cttcttcaat atatgtatgc aagctattaa atttgtccat
     1621 aacttcacct cgtgatatca ttctatataa gttatcgtca tgttttataa aaacattaga
     1681 aaaatttata taataccggt taagctatgg ggcataaaaa aacagccgat agatttctct
     1741 attgactgtt tagtatttct ctttttacga gattacatca ttcccattcc gccgcccatt
     1801 ggtggcattg caggttcttc ttttttaggt tcgtctgtta ctgccgcttc tgttgtcaat
     1861 aacattgcag ctacgcttgc tgcattttga agggctgatc ttactacctt tgttgggtct
     1921 acgatacctg cttcaatcat atttacatat ctttcgttta atgcatcaaa tccgacacct
     1981 ttttcgctat ttttgatttt ttcaactatt actgagcctt caagacctgc attgattgca
     2041 atttgtctga gtggttcttc caatgctttt tcgattatct tagcacctgt ctttgcatct
     2101 ccgtccaatc cttctgctac cttgttaact ttatcgattg ttgttaaaag tgcaacaccg
     2161 cctcctgata ccaagccttc ctcaactgct gctcttgtag catttaaagc atcttcaatt
     2221 cttaatttct tttctttcat ttcggtttct gtagctgctc caacattgat aactgccact
     2281 ccgcctgtta atttagctaa tctttccatc agcttttctc tgtcgaattc agaagttgat
     2341 tcatcgtgct gagctctaat ttgtctagct ctttcttcga tttcttcctt agaacctgca
     2401 ccgtttacta ttacggtatt ttctttgtct atcttaacag ttccggcatt accgagcata
     2461 tctaaggttg tttcttttaa ttctaatcct aactcttcgg atattacctg gcctcctgtc
     2521 agaattgcga tgtctcttaa catttctttt cttctgtcgc caaagcccgg tgcttttacc
     2581 gctacacaat taaaggttcc tcttaattta tttactacca atgtagctaa tgcttcccct
     2641 tctacatctt ctgctataat aagtaatgat ttgcctgttt gaacgatttg ttccaaaacg
     2701 ggaagaacat cctgaatgtt tgtaattttc ttgtctgtta ttaaaatata cggatcttct
     2761 aaaacagctt ccatcttttc tgtattagtt accatatatg ccgataagta ccctctgtca
     2821 aactgcatac cttcaacaac gaccagctct gtatccatag tctttgattc ttcaacagtt
     2881 ataacgccgt ccttgccaac ctgatccatt gcttgtgata ttaattttcc tatttcttca
     2941 tctcctgcgg aaatagaagc aatttgagca atttcttctg atgtttcaac tttttttgtt
     3001 atgtttttaa gttcttccac tgctgctgca actgcttttt cgataccttt tttaagtatc
     3061 atgggattag cgcctgctgc aacatttttg agtccttctc ttattattgc ctgtgccaat
     3121 aatgttgctg tagtagttcc gtcacctgct acgtcatttg tttttgttgc aacttctttt
     3181 accagttgtg ctcccatgtt ttcatacggg tcagaaagtt caatttctct tgctattgta
     3241 actccgtcat tagtaatctg cggtgagccg aatgatttgt ttaaaacaac atttcttccc
     3301 tttggcccta aggtaacttt taccgtatct gctaatgcat taacgcctct ctccattgcg
     3361 cggcgtgcat cttcatcata tttaattatt tttgccatat ttatctctcc tttttattca
     3421 ataactgcca atacatcagc taattttatt ataattaatt cttcaccgtc aattttcact
     3481 tcagttcctg caaatttaga gtaaataacc ttgtctccta ctttaatttc atcttttttc
     3541 ttttcatcgt ttgtaattcc cgcaccaaca gcaagaactt cagcaacctg cggtttttct
     3601 tttgcagagc cgggcaatac aatgccgctt ttggtttttt cttcggcttc aatcatttta
     3661 attactactc tttcgcctaa tggttttact gtcattatca attcctccta tatttaatgt
     3721 taatatatac gattttttgt tagcactcaa ctccaaagag tgctaacacc tattctttat
     3781 attactcatt tgcctttgtg ttttcaaccc ctatttaccc caattaacac ccataaacat
     3841 attaatatat aattattcct accgttttct ttcctttgct ttaaattgct ccatatttca
     3901 taacaaaaga ccgtgacaag gggacggggg tattgtcata tgacaagggg acggggcgtt
     3961 gtcataaaat atattttaaa taatagatgt tttttatgcc gatacgttgt ataatgaatt
     4021 taccagtata atacatagga gggtttaaaa tggctcttgt aacatcacaa gaaatgttta
     4081 agaaagcata tgagggcaaa tatgctgtag gtgccttcaa tgtaaacaac atggaaatca
     4141 tacaaggaat agtggaagca gcaaaacagg aaaatgcacc tcttatactc caagtttcgg
     4201 cgggcgcaag gaaatatgca aatcctattt atttaagaaa gcttgtggaa gcagcaattg
     4261 aagacacagg attagatatt tgtcttcatc tagaccatgg tgaagatttt gaaatttgta
     4321 agcaatgtgt tgatgatggt ttcacctcag ttatgattga cggctctaaa tatccttttg
     4381 aagaaaacat agctcttacg aaaagagtgg ttgaatatgc tcactccaaa ggggttgtag
     4441 tagaagctga gctgggtaaa ttggcagggg tagaggatgc agttaaagta agaaccaaag
     4501 atgctacata cacagaccct gatgaagctg tggaattcgt ggaaagaact ggagtagact
     4561 cattggcaat tgctatcgga acaagccatg gtgcttataa attcaagggc gacccaactt
     4621 tggactttga aagattggaa accatatcaa atcttctccc gggttttccg ttggtactgc
     4681 atggttcatc ctcggtacca aaggagtttg tggatttatg caataaatac ggaggtcaaa
     4741 tcccgggagc tgcaggagtg cctgaagaaa tgctaagacg tgccgctcaa ttaggggtat
     4801 gcaaaataaa tatagataca gatttaagac ttgctcttac cgcatgtatc agacaatttt
     4861 ttgttgaaca tccttctgaa ttcgacccta gaaaatattt aggtcccgga agaacagcga
     4921 ttaaagatat ggttgctcac aaaatgagaa atgttttggg atgcagcaat aaaagataga
     4981 taactgacaa ggggacgggg gtattgtcat cgttgagatg acaatacccc cgtccccttg
     5041 tcattatttt aaatcattca tatatgttga aacataattt ttgtcaactg tgacaacggc
     5101 gaaatatgtt tcatctttat tttgtatttc cttttctatc atactcacta gttttgatga
     5161 ttttatatca tagtccaccg gtacaaccac atcaaatatt aaattggtat gggtttctcc
     5221 cttcactact ctaaaatcat gcatggtaag tctttcatca atggatttta ctattttatc
     5281 cgtcatgctt ctcaatccat ttatttcctc atcatccgta ataacggggt ctaaatgaat
     5341 taccaggctg atatcaaatt ctcttgcaaa atccctttca atgttatcga ttaaatcatg
     5401 agatttcatt atatcttcct tcgcatcaac ctctgcatga actgatgcaa agtatctgtt
     5461 aggtccgtaa ttgtggacta caaggtcatg aatatttact attccatcat aggataaaat
     5521 cttattctca attgattcaa taaattcttt atcaggcatt tctccaagaa gcggattcaa
     5581 tatatctctt aatatgtcat aacccgaata taatatgaat aaagccacta ttattcccac
     5641 ataaccatca acttcatagc ctgttgcttt ggataccaat aaggataaaa ttacaactat
     5701 tgttgaaatt acatcattcc tcgaatccgt ggaagaagcc ttgagggttg ctgatgaaat
     5761 tttatcgcca agttttttat agaataaact aatccataac tttattaaaa cagaaacaat
     5821 aagtacgact atcatctcaa tggaaaatat ggtttttgtc gggttcatga ttttatcaaa
     5881 ggatgattta ataagctcta caccgactaa aatcacggcg attcctaata tgagccctgt
     5941 caggtattcc attcttgcat gaccgaaagg atggtccttg tcggcaggtt tgcttgaaat
     6001 tttaaaacct ataagggtaa ttacagaaga tgcaccgtcg gataaattat tgactccgtc
     6061 agcagtaaca gaaatactgt taaatattat acctgtcagt attttcccta tacttaaaag
     6121 agtatttaaa ataatacccg taatgcttgc cgttatgccg taggattctc ttaccctttg
     6181 ctcattaaca ttttcatagt ctttaataaa ttttttaata atgaaatcag tcatcgcatt
     6241 tctccaactt taataagctc tctaattagc cttaaataaa ttatatgcat tatataatta
     6301 tattcttcta aatttaattc atacataatc aatatgaacc aactgttttc ttcttgcgcc
     6361 gcagaaattt agcattcttg tcatatgcaa atacctgtgg gagtattccc acaggtataa
     6421 attactataa tatagtattt ttgtcaaatt aatgtttcca tctttctttt gagtcattgc
     6481 tgttatgact gtcttcatca tccttatttt cgtttttgta cgatttatat gatgtgtctt
     6541 ttttattgtg atttttattt tctgtttctt tttgcttttg tcttaattct tccctgtatt
     6601 ctcttatctt agcatttatc ttctgga
//
LOCUS       DAOSOW010000055         6584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_11778_length_6584_cov_3.273089, whole genome shotgun sequence.
ACCESSION   DAOSOW010000055 DAOSOW010000000
VERSION     DAOSOW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11778_length_6584_cov_3.273089"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..773)
                     /locus_tag="PLD67_02430"
     CDS             complement(57..773)
                     /locus_tag="PLD67_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21416.1"
                     /translation="MAKKIIGYGNFFCWNCGNRIEKRKKTCPNCGSIYSGDGKYGNVQ
                     ALGAGGIGWSNNVNHYSLKKYFKNDRKYSFIWLIGISIIVPAIMLLSGEIDFDSEGIM
                     VIGGILAVFWGTGLLFIFKKGANEPDWDGIVKDKKVFQKTRRKKDSEGKAYTEEYKEF
                     IVYIRKQNNDIFELKDEDSARYDYFNIGDYLHYHGVKYLNYFEKYDKSLDTIIFCASC
                     RNICDIRDNYCERCGCILLN"
     gene            complement(800..1339)
                     /locus_tag="PLD67_02435"
     CDS             complement(800..1339)
                     /locus_tag="PLD67_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21417.1"
                     /translation="MNTIKRKIIIYILIIFFLTAALLQLFSLQSEKLVLHIMVLIIFI
                     IAADGIFIYLLIDKINNLKIARLITENFILKVKTAVISEISVKEAPNKLKCLEDTEIF
                     VSYFGILFNGKIAKFNQDGIRLKEMAIGSDFISFTYGNEKQMQNLRIVRPDIEPEALN
                     EMINKFRFETGITPTIIEG"
     gene            complement(1477..2901)
                     /locus_tag="PLD67_02440"
     CDS             complement(1477..2901)
                     /locus_tag="PLD67_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013779938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HOT21418.1"
                     /translation="MTNNDKQRNILFFDLNERNLAVMVHSLFSSWMLSFLFEGQIFQQ
                     LAKEYNFASESIIIWSSAAMFSGLLLGGFTVKSKLHAKRLFLFSYPFFIALSLIFFFS
                     PSILWTMGIITGSIVSGCCIAAWGFYFKSVSEKNERIKTAADMLIISNILMILLNVVA
                     INISPNVGLTLSLVMLIFAFILGLRLPTDYNEDYGNKKDGSLSISNLLLFFYIFIVIL
                     TINSGLMYHVVNPAFEHHATLTSWYWAVPYVAVILILRIFSQKIMSSYILYVAIAMIG
                     LSFIAFIALDRSAISYFIINTLMLGSYGVYDLFFWSIFGEMLDYHKNPAKILGTGLSA
                     NVLGIVAGGLIGNSLMANNLYSINPSLFAFAVVCVSLVLLPLLNKYLSTLLKGYTFLT
                     AFVVMPEFKQNSQIDKISEYGNLSEREKQVALLLLQGKTYKTIAGELFISENTVKYYV
                     KNIYSKFNIQSRAELIDMVAEKQN"
     gene            complement(3065..4072)
                     /locus_tag="PLD67_02445"
     CDS             complement(3065..4072)
                     /locus_tag="PLD67_02445"
                     /inference="COORDINATES: protein motif:HMM:NF016983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HOT21419.1"
                     /translation="MDKRPYLNFFVTVLVLLLISFVIIMINQAAQLVGIAANLHPYLG
                     TGLLIFFVIFGLAAAVYVVLLLSRFEKPLEIPSEENSEEYDQYILKLKNRLKKNKFLN
                     KSQYVWDDSKPDIESVNDALIKIDQEAQKIIKGSAAGVFTTTAVSQNGSLDGIFVFVV
                     SMKLIWKISTLYNQRPAMRDLLKLYTNVFATVLAARQIDDLDIVAEQLGQILPAVMTG
                     ALGSVVPGVSFITSFVVDSILEGTINTLLVLRIGLLTQLYSRSITRTEPKRLSKAATV
                     QACKMLGEIISKDLISIIGIWSKSAFKAVTKVPRNTKDTFMDFFKIKSSDEEAAITDI
                     D"
     assembly_gap    4358..4457
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4458..6129
                     /locus_tag="PLD67_02450"
     CDS             <4458..6129
                     /locus_tag="PLD67_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT21420.1"
                     /translation="LFYNIILTQALAYIILWLVVVPQRNGEECDFMVTNDYQKEYDAV
                     VIGAGNGGLTAAATLAGKGMKTLLIEQHNLPGGFATSFVRGRFEFEPSLHEISDFGPP
                     EDKGATRQLLEDKIGLDLEWLRVPEAYRLIIPNEKGGRLDVVMPFGKEEYISKLEEYV
                     PGSRPYVTDFISLCEEVVNAFTYLAESKGKADKNVLMDKYPNFLKTASYSLQDVLDAM
                     KIPYLAQKILTAYWCYLGLGTKQVNFTIFAAMFYKYVMRAAYVPKYRSHGFTVALDKR
                     IRELGGEILYNTKVEKILVEDGKVVGVETSKGDKIKTQFVISNASPHLVYNQLIYPKE
                     EIPDIALKTCNARKVSFSAFVVYLGLNKSPQELGINEYSYFVYSTPDTNDIYKQFHEL
                     RRPTGQATVCLNNAIPDCSPEGTSIVYLTTLYSGDCWSKVEPKDYFELKNKIAEEMID
                     EFEKALGINIRDSIEEIEVATPATFASYTGAFNGSVYGYEPVPWDSVLPRMMCMNDEK
                     YIKGLEFAGGFAFRCHGYSSSLQSGETAALLTYKDFMEVKNNEKTVEH"
     gene            6107..>6584
                     /locus_tag="PLD67_02455"
     CDS             6107..>6584
                     /locus_tag="PLD67_02455"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOT21421.1"
                     /translation="MRKQLNIKIKPLGFLDLLAFKNLIPTRKKNINNATADLIEAAPP
                     VNVLAQRLHPKAQHFVIEDVTFETSSAKTFRLVPDEESETGELAFFRPGQYLTFSFKI
                     NGSTVKRPYSISSSPNETLKGYYDITVKRDEGGFVSSYIFDNWDKGTKVTSSGPEGH"
BASE COUNT         2076 a         1237 c         1098 g         2073 t
ORIGIN      
        1 cagagtcatc gggaagctgt gtcccgcaat tttcacaaaa cattgttttc ctccttttaa
       61 tttaataata tacatccgca cctttcacaa taattatctc gtatgtcaca aatattgcga
      121 cagcttgcgc aaaaaattat tgtatccaag gacttatcat acttttcaaa gtaatttaaa
      181 tatttgaccc catgatagtg cagataatca cctatattga aataatcata ccgagcacta
      241 tcctcatcct ttaattcaaa aatatcattg ttctgcttgc gtatataaac aataaattct
      301 ttgtattctt cggtataagc cttcccttcg ctgtcttttt ttcttctcgt tttctgaaat
      361 acttttttgt ctttaacaat tccatcccag tccggctcat tagctccttt cttaaatata
      421 aataaaagac ctgttcccca gaataccgcc aaaataccgc cgataaccat aataccctca
      481 ctgtcaaaat ctatctcccc tgaaagaagc attattgcgg gaactattat actgatgcct
      541 atgagccaaa taaagctgta ctttctgtca tttttaaaat atttttttaa actgtaatga
      601 tttacattgt ttgaccagcc tatacctccc gctcctaatg cttgaacatt cccgtacttg
      661 ccatctccgc tgtatatgga gccgcagttt ggacacgttt ttttcctttt ttcaattcta
      721 ttgccgcaat tccaacaaaa aaagtttccg taaccaataa tttttttagc catattctac
      781 ctcattgcaa tatatttctt tatccctcga ttatcgtcgg tgtaatcccc gtttcaaaac
      841 ggaatttatt aatcatttca ttcaaagcct caggctcaat atccggccgt acaatacgca
      901 aattttgcat ttgtttttca ttgccgtagg taaatgatat aaaatcactc ccaatggcca
      961 tttctttcag ccggatgcca tcttgattaa attttgcaat tttaccgtta aagagaattc
     1021 caaaatatga tacaaatatt tctgtatctt ctaagcattt aagcttattt ggcgcttctt
     1081 tgactgaaat ttcgctaata actgcagtct tgaccttcaa tatgaagttt tcggttatca
     1141 gtcttgcaat tttcaaatta ttaattttat ctatcaagag gtatataaat ataccatcag
     1201 cagcaattat aaatattatc aataccatga tatgaagaac aagtttttca ctttgaagag
     1261 aaaaaagctg gagtaaagct gccgtcaaaa aaaatattat taaaatgtaa ataatgattt
     1321 ttctttttat tgtattcatc ccaaccaccc gaaactgcat tgttattgtt acagaattct
     1381 tacttgatta gagattataa cagttaaact gcaaaaaaaa atcacccgaa aagggtaatt
     1441 ctttaaaaaa caaccctacc tttttcgggt aggtgtttaa ttttgttttt ctgccaccat
     1501 gtcaatgagt tctgcccggc tttgaatatt aaatttggaa taaatatttt ttacgtaata
     1561 ttttacggta ttttcgctta taaaaagctc acccgcaata gttttgtagg tttttccctg
     1621 tagaagaagt aatgccacct gtttttctct ttccgacaaa ttgccgtatt ctgatatttt
     1681 gtctatttga ctgttttgtt taaattccgg cattacgaca aatgcagtaa gaaatgtata
     1741 tcctttaagc aaagttgaca ggtatttgtt cagaagaggc aatagtacca aagatacaca
     1801 aactaccgca aaggcaaaca gtgatggatt tatgctgtat aaattatttg ccattaagct
     1861 attgcctata agtccgccgg ctactatacc caatacattg gcagaaagac ctgtgccaag
     1921 aattttggca gggtttttat gataatcaag catctcccca aatatgctcc aaaaaaacaa
     1981 atcataaaca ccataggaac caagcataag tgtatttatt ataaaataac ttattgcaga
     2041 gcggtcaagg gcgataaaag caataaatga aagaccaatc attgcaattg ctacatatag
     2101 tatatatgat gacattatct tttgagaaaa aattctaaga atcaatatta cggcaacata
     2161 gggaactgcc caataccagc tagtgagtgt tgcatggtgt tcaaaggcgg gatttaccac
     2221 atggtacatt aatccggaat ttattgtcaa tattactata aagatataaa agaagagtaa
     2281 aagatttgaa atgcttaagc tgccatcctt tttattaccg tagtcctcat tgtaatccgt
     2341 gggtagtctt aagcccagta taaatgcaaa tattaacata acaagagaca aggtaagccc
     2401 tacattggga gatatattta ttgcaactac atttaataat atcatcaata tattggaaat
     2461 aatcagcatg tctgctgctg tttttattcg ttcattcttt tctgatacac ttttaaaata
     2521 aaatccccat gcagcaatac agcatcccga aacaatggaa ccggttatta tgcccatagt
     2581 ccatagtatt gaaggcgaaa agaaaaatat taaggacaag gcgataaaaa agggataaga
     2641 aaagaggaaa agccgttttg catgaagttt actttttaca gtaaatccgc caagcagtaa
     2701 tcccgaaaac atagcggcag aactccaaat aattattgac tccgatgcga aattatactc
     2761 tttggcaagt tgttgaaaaa tttgcccttc aaaaagaaat gacagcatcc atgaagagaa
     2821 caaggaatga accattacag ccaaattgcg ttcattcaaa tcaaagaaga gaatatttcg
     2881 ttgtttgtca ttatttgtca tcttttctgc ccccctgaac attcacagat acttatcata
     2941 caaacaaata tttcaattga atttcgcatg tataaaatat attataatag attatttaat
     3001 aattgacaat ccccctactt aaaaaaggta gggggatttg agttgcaaac ctatctttgt
     3061 tatactaatc aatatcagta attgccgctt cttcatcaga gctttttatt ttgaaaaaat
     3121 ccatgaaagt atcctttgta tttcttggaa ccttggttac tgccttaaaa gcggactttg
     3181 accatatacc gattattgat attaaatcct ttgatattat ttcaccaagc attttacaag
     3241 cttgtacagt tgcagcctta ctcagtctct taggctctgt tcttgtgatg ctcctgctgt
     3301 ataactgtgt taacagtccg attctcagta ccaacaaggt gttgattgtt ccttccaata
     3361 ttgaatcgac tacaaaggat gtgataaagc tcaccccggg cactacactt ccaagtgctc
     3421 cagtcataac agcaggaagt atttgaccaa gctgttcagc aacaatatcc aaatcgtcta
     3481 tctgtcttgc agcaagcacc gtggcaaata catttgtata aagcttcagt aaatctctca
     3541 tcgccggcct ctgattatat aatgttgata ttttccaaat cagtttcatg gatactacaa
     3601 atacaaaaat cccgtccaat gaaccgtttt gtgaaactgc cgttgtggtg aatactcctg
     3661 ccgcactgcc ttttattatt ttttgtgctt cttggtcaat ttttatcaat gcgtcattta
     3721 ccgattcgat atccggctta ctgtcatccc atacatattg tgacttattt aagaatttat
     3781 tcttctttag tctatttttt aattttaaaa tatattggtc gtattcttct gagttttctt
     3841 cacttggtat ttccaaaggc ttttcaaacc tggatagcag taatacaaca tatactgctg
     3901 cagctaatcc aaaaataaca aaaaatatca ataggcctgt acctaaatag gggtgtagat
     3961 ttgcggcaat gcccactaat tgagcagcct ggtttatcat tataataaca aaggaaatca
     4021 acaataatac taatactgta acaaaaaaat tcaaatatgg tcgtttgtcc ataatatctc
     4081 ctaactatag tgtaataaat attatttttt ttgtattaat gtaaaatcat aatagcacca
     4141 atgtaatttt ttgtcaaaaa ttataatcaa cgtttttcat aaggaatcat tttttaggca
     4201 ctgcttattt aaacaacaat tccctgttac caacagtaat atccgattgt ttttttggtt
     4261 gaagttttta ttgtgattct gacgtatgtt taatgctttt ccattatttt acaatatcat
     4321 attgacacaa gctctggcat atattatact gtggttgnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnatt attttacaat atcatattga cacaagctct ggcatatatt
     4501 atactgtggt tggtggttgt accacaaaga aatggagagg agtgtgattt tatggttacg
     4561 aatgactacc aaaaggaata tgatgctgtg gtcataggag ccggcaatgg cggcctcact
     4621 gctgcagcaa ccttggcagg caaagggatg aagaccttac tcatagagca gcacaacctg
     4681 cccggtggtt ttgcaacaag ttttgttaga gggagatttg aatttgaacc ttcacttcat
     4741 gaaatctctg acttcggacc tcctgaagac aaaggcgcta caagacagct cttggaagac
     4801 aaaataggct tggaccttga gtggctaagg gttccggaag cctacaggct tattataccc
     4861 aacgagaaag gcggcaggct tgatgtagtt atgccttttg gcaaagaaga gtatatatca
     4921 aagttggagg aatatgttcc gggttcaaga ccctatgtaa ctgattttat aagtttgtgc
     4981 gaagaagtgg taaacgcttt cacctatctt gccgaatcaa aaggaaaggc cgataaaaat
     5041 gtcttgatgg ataagtatcc taatttcctt aaaactgcct catattcatt gcaagatgtt
     5101 ttagatgcaa tgaaaatacc atatttggca caaaaaatcc taacagctta ctggtgttat
     5161 ttgggtttgg ggacaaagca ggtgaacttc accatctttg cagccatgtt ttacaagtat
     5221 gtaatgcggg ctgcttatgt tcccaaatac cgttcacacg gatttacggt tgcccttgat
     5281 aaaagaatca gggaattagg cggagaaatc ctgtataata ccaaggttga aaaaatcctg
     5341 gtcgaagatg gtaaagtggt cggtgttgaa acctcaaaag gagataaaat caaaacgcaa
     5401 tttgtaattt ccaatgcttc accccatttg gtttacaatc agctaatata tccgaaagag
     5461 gaaattcctg atatcgccct gaaaacctgc aatgcaagaa aggtaagttt ttctgccttt
     5521 gttgtatatt taggtctcaa caaaagccct caggaattag gtatcaatga atacagctat
     5581 tttgtttact ccactcccga taccaatgat atctacaagc agtttcatga attgagacgg
     5641 cctacgggac aagcaaccgt ctgcttgaat aatgccattc ccgattgctc cccggaagga
     5701 acaagcattg tgtatctgac caccctttat tccggagatt gttggagtaa ggtagagcca
     5761 aaagattatt ttgaactgaa aaataaaata gctgaagaaa tgatagatga atttgaaaaa
     5821 gcgcttggaa tcaacataag agacagcatt gaagaaatag aagttgctac tccggcaacc
     5881 tttgcctctt acactggagc ctttaacgga agcgtctacg gttatgagcc cgtaccctgg
     5941 gattctgttc tgcccagaat gatgtgcatg aatgatgaaa aatacataaa aggccttgaa
     6001 tttgccggag gctttgcttt caggtgccat ggttactcaa gttctcttca gtccggtgaa
     6061 acagcagctt tattaactta taaggatttt atggaggtga aaaataatga gaaaacagtt
     6121 gaacattaaa attaagccct taggtttttt ggatttattg gcatttaaaa acttaattcc
     6181 aaccagaaag aaaaatatca acaatgctac agctgattta atcgaagccg cacctcccgt
     6241 aaatgtatta gctcaaaggc ttcatccaaa agctcaacat tttgttattg aggatgtaac
     6301 atttgaaact tcatcagcta aaacttttag gctggtgccg gatgaagaat ccgaaaccgg
     6361 cgagttagcc ttcttcagac ccggtcaata cttaactttt tcttttaaaa ttaacgggtc
     6421 tacagttaaa aggccctact ccatatcctc ttctcccaat gaaaccttaa aaggttacta
     6481 tgatataacc gttaaaaggg atgaaggcgg atttgtttcc tcctatattt ttgacaattg
     6541 ggataagggt acaaaagtaa cctcgagcgg tcccgaaggc cact
//
LOCUS       DAOSOW010000056         6561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_11832_length_6561_cov_3.374885, whole genome shotgun sequence.
ACCESSION   DAOSOW010000056 DAOSOW010000000
VERSION     DAOSOW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11832_length_6561_cov_3.374885"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1922)
                     /locus_tag="PLD67_02460"
     CDS             complement(<1..1922)
                     /locus_tag="PLD67_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HOT21422.1"
                     /translation="MTASNDDAIRMLDEEIRYLRLLSKQYPTIASACTEIINLQAILN
                     LPKGTEHFLSDIHGEYESFNHVLRNGSGNIKRKINEVYGDIMSEKEIKSLATLIYYPE
                     QKLEIIRKEEEDIEDWYRITIYRLVVICRKAAFKYTRMKVRKALPKEFAYIIEELMHK
                     GPEEDKEEYYSEIINTIIRIGRADEFIIAMAKLIQRLVIDRLHIVGDIFDRGPGADKV
                     MDTIMNYHTVDIQWGNHDVLWMGAAAGSETCIATVLRICARYANLDIIEDGYGINVLP
                     LATFALEYYGDDECIQFKPKVESDIVYSDKDLKLIAKMHKAIAIIQFKLEGHIIKRRP
                     YFKMDDRLLLEKINYEEGTIDLYGKTYHLNDRNFPTIDPKNPYALTKEEEELVEKLKS
                     SFLNSEKLQKHIRFMLSNGSMYLKFNSNLLYHGCIPVNEDGTFKKVTVGRTGKAYSGK
                     EYFDRLEILVREGYFHKTNPEAKQYGMDITWYLWTGPDSPLFGKDRMTTFERYFIDDK
                     KTHKETKSPYFKLEDSEEMCRRIFEEFGLNPECSHIINGHVPVKSKSGESPIKANGKL
                     IVIDGGFSRAYQSTTGIAGYTLIYNSYGLLLVSHDPFESTQKAIEEEKDIHSTTMVLE
                     KEVERKRVKDTDAGEV"
     gene            2155..3231
                     /gene="buk"
                     /locus_tag="PLD67_02465"
     CDS             2155..3231
                     /gene="buk"
                     /locus_tag="PLD67_02465"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOT21423.1"
                     /translation="MKNSYKILAINPGSTSTKIAVYENEKLIFKETISHSNSDLEKFD
                     KITDQFNYRKDIILDTIKKQNFDLSELDAIVGRGGNFKPVEGGTYKVNKEMINDLIIG
                     VMGHHASNLGAIIADAIANDLGLSAYIVDPVVVDELEDYARISGMPEIERKAKAHPLN
                     QKAVARLAAAELGGKYSDYNFVVAHMGGGISIGAHKNGRIVDINNCLDGEGTFTPERA
                     GGLPVGSLVEMCFSGKYTIQEIKKKITGKGGLVAYLGTNDAMEVKKRIAEGDKYAKLI
                     YETMAYQIAKDIGAAATVLKGKIDNIILTGGLAHDDVLVQWIKERVEFIAPVKVYPGE
                     FEMQAMVQGVIRVLTGEEELKIYR"
     gene            complement(3260..3421)
                     /locus_tag="PLD67_02470"
     CDS             complement(3260..3421)
                     /locus_tag="PLD67_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21424.1"
                     /translation="MINLKRFLKYRDNAPEFKEIKDEGRVGLPCIVIENGEEILFDYN
                     LLDIESLKE"
     gene            complement(3677..4142)
                     /locus_tag="PLD67_02475"
                     /pseudo
     CDS             complement(3677..4142)
                     /locus_tag="PLD67_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019910707.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
     gene            complement(4324..4599)
                     /gene="spoIIID"
                     /locus_tag="PLD67_02480"
     CDS             complement(4324..4599)
                     /gene="spoIIID"
                     /locus_tag="PLD67_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HOT21425.1"
                     /translation="MKDYIERRAMEIAEYIISTQSTVRQTAKKFGVSKSTVHKDVTER
                     LPKLNPPAANEVKKVLLKNKSERHIRGGKATRLKYKEAQSQEKVSNM"
     gene            complement(4676..5395)
                     /locus_tag="PLD67_02485"
     CDS             complement(4676..5395)
                     /locus_tag="PLD67_02485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT21426.1"
                     /translation="MKNNRFARAKEKMKDFFIRLRHKDTSFLIIALCIMLLATAVVWR
                     YTSNPENGQSDLAENNEPNGDIKVHEDPYEDYVQDIIDEFQNAEDDNDEDDGVKPEGT
                     DLKSMKSPLAGEIIREFTLDELVYYEAIGEWRVHKGIDIKPKDTLLIESALAGKVEAV
                     NNSEITATEIIIDHGNNVKTLYSNLVSANVKAGDTVEKGQVIGNVGKTVSIEASDGAH
                     LHFEVIVDGQHINPLDYITIE"
     gene            5532..>6561
                     /gene="spoIID"
                     /locus_tag="PLD67_02490"
     CDS             5532..>6561
                     /gene="spoIID"
                     /locus_tag="PLD67_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HOT21427.1"
                     /translation="MLNRIIYAFVVFTMLLTIPNLSLNFFEKKMNLTSAAVQQKDSSK
                     YADEKHEEIYTADVYEPELIKVYNNKTNEVMIIDFEEYLKGVVASEMPAEFNIEALKA
                     QAVSARTYLLYRLKKYPDGQPEHSEAPVCTGVHCQVWNSKDELIRSHEEGWYDESWGK
                     IEEAVNSTKGQILVYDNKIIEPLYHSTSGGRTENSEDVFSTAVPYLRSVESPYEGDSP
                     KLNGSIKITTSEFIDKIESAYGDMDITESNLADKIVLGEVSEGGKIKTLIIDNTEISG
                     RDMRALFNLNSTNFSFIQSGNEIEILTTGYGHGVGMSQWGAEGMANRGYNYKGILKHY
                     YTGVEIVSM"
BASE COUNT         2023 a         1195 c         1097 g         2246 t
ORIGIN      
        1 ataacttctc ccgcatcggt atccttgact ctttttcttt ctacctcttt ttccaaaacc
       61 atagttgttg aatgaatatc cttttcttct tctatggctt tttgcgttga ttcaaagggg
      121 tcatgagata ccaagagcag tccgtaggaa ttatatatga gagtgtatcc ggcaatacct
      181 gtagtacttt gatatgctct tgaaaagccc ccgtctataa ctattaattt tccgtttgcc
      241 tttataggac tttctccgct tttgctttta acaggcacat gaccgttaat aatgtgagag
      301 cattccggat tcaagccgaa ttcctcgaaa attcttctgc acatttcttc actgtcttct
      361 aatttaaaat acggactttt agtttcttta tgtgttttct tatcatctat gaaatatctt
      421 tcgaaggtag tcattctatc ttttccgaac aagggagaat caggaccggt ccacaaatac
      481 catgtaatat ccataccgta ttgtttagct tccggattgg ttttatgaaa atatccttct
      541 ctgactaata tttcaagtct gtcaaagtat tcttttccgc tgtatgcctt ccctgtacgg
      601 cctaccgtta cctttttaaa ggtaccgtct tcattaacgg gaatacaccc gtgatataaa
      661 aggttcgaat tgaatttcag atacatgctt ccgtttgaaa gcataaatct tatatgtttt
      721 tgtaattttt cgctgtttaa aaaagatgac tttaactttt caaccaattc ttcttcttct
      781 ttagtgagcg catacgggtt tttagggtca attgtcggaa agtttctatc attcaagtga
      841 tatgtcttgc catataaatc aattgttcct tcttcataat ttattttttc aagcagcagc
      901 ctgtcatcca tcttaaaata cggacgtctt tttattatgt gaccttccag cttaaactgt
      961 ataatggcaa tggctttatg catttttgca attagtttta aatccttatc tgaataaacg
     1021 atatcacttt ctaccttagg tttaaactga atgcactcat catcaccgta atattccagt
     1081 gcaaatgttg ctaagggaag aacatttatg ccgtatccgt cttctatgat gtctaaattt
     1141 gcatatctgg cacatattct taatactgta gcaatacatg tttcacttcc ggcagcagca
     1201 cccatccaca atacatcatg gttgccccat tgtatatcca cagtatgata attcataata
     1261 gtatccatta ctttatccgc accgggacct ctgtcaaaaa tatctcctac aatatgaagc
     1321 ctgtctataa ccaatctttg aataagcttt gccattgcaa taataaattc atcagctctg
     1381 cctattctta ttatcgtatt tattatttcg ctgtaatatt cttctttatc ttcttcaggc
     1441 cctttatgca tcagttcttc aattatgtat gcaaactctt tcggaagggc ttttcttacc
     1501 ttcatgcgag tatacttgaa agctgctttc ctgcaaatca ccacaagcct gtaaatagta
     1561 attctatacc agtcttcaat gtcttcttct tccttgcgaa ttatttcaag tttttgttcg
     1621 ggataataaa tcaatgtggc aaggctttta atttcctttt cactcataat atcaccatag
     1681 acctcattga ttttacgttt aatattgcct gagccgtttc ttaaaacatg attgaatgat
     1741 tcgtattccc catgtatatc ggatagaaaa tgctccgtac ctttgggtag atttaaaata
     1801 gcttgtaagt ttataatttc tgtacatgca gaagctattg tcgggtattg ttttgataaa
     1861 agccttaaat atctaatttc ctcatctaac atacgaattg catcatcatt agaagcagtc
     1921 atgatatttc tccttttatt atatttccac aacggatatt acctatatat agtttataac
     1981 ataaatggaa taaaagccat aaatttttaa atttttatta taaacttgag ttcttttctt
     2041 gtgcggctta ctttaacctt cttaacagaa tttgtataat aggttttgta aattccaaaa
     2101 attttgatat aatcaaagaa aaagaatata gttgtaccta tatcgggagg acttatgaaa
     2161 aactcgtata aaattttagc tatcaatccg gggtctacct ctacaaaaat cgcagtatat
     2221 gaaaatgaga aattaatatt taaagaaaca atatctcata gcaattcaga tttggagaaa
     2281 ttcgataaaa ttactgacca gtttaattat cgaaaagata taatactgga cacaataaaa
     2341 aaacaaaact tcgaccttag tgaactggat gcaattgtag gaagaggcgg taactttaag
     2401 cccgttgaag gcggtactta caaagttaat aaagaaatga ttaatgattt aataattggt
     2461 gttatgggac accatgcttc aaatctgggt gccataatag ctgatgcaat agccaatgat
     2521 ttgggattat cagcatatat cgttgacccg gtagtcgtag atgaacttga agattatgcg
     2581 agaatttcag ggatgcctga aatagaaagg aaagccaaag ctcatccttt aaatcagaag
     2641 gcagtagcca ggctggcagc cgccgaatta ggaggaaagt attccgatta taattttgtt
     2701 gttgcacaca tgggtggagg tatttcgatt ggagcacata aaaacggaag aatagtagat
     2761 ataaataatt gtttggatgg cgaaggaaca tttactcctg aaagagccgg cggattaccg
     2821 gtaggctcat tggttgaaat gtgcttttcg ggaaagtaca caattcaaga aataaagaaa
     2881 aaaataaccg gcaaaggcgg cttggtagca tacttaggca caaatgatgc tatggaagta
     2941 aaaaagagaa tagcagaagg agataaatat gcaaaactta tatatgaaac tatggcatat
     3001 cagattgcta aagatatcgg agcagcagca acggttctaa aggggaaaat tgataatata
     3061 atattgaccg gcggattagc acatgatgat gtcctggttc aatggataaa agaaagagtt
     3121 gaatttatag cgcctgtaaa ggtatatccc ggagaatttg aaatgcaggc aatggtacaa
     3181 ggtgtaataa gagtattaac cggagaggaa gagttaaaaa tatacagatg atttaaaaac
     3241 ccaccatgat gtgggttaat tattctttta aactctctat atccaacaaa ttataatcaa
     3301 acaggatttc ttctccgttt tcgattacga tgcaaggtaa cccaaccctg ccttcatctt
     3361 taatttcctt gaattcaggc gcattgtccc tgtatttcaa aaatcgttta agattaatca
     3421 tattttctga tatatccaga tatatgtaat taacttcatt ttccgaaaga aactctttcg
     3481 caggttcaca atccggtcaa tgtttgcttc caaacacaat tactttcttc attcgcttct
     3541 ccttatatta aatttaccat aaaaatagaa tgttattagt aattaacacc tctctaacaa
     3601 atttctattt ttgtattatt tatcattata agacaattga taaaattata caataataaa
     3661 tgcttcaatt ataaacttaa cacgaatgct ggcaatcagg gcaatagaag catttacgag
     3721 aattgagttc tattttaaca atagtacctc cacatcgact acatttcttg ccctctcttc
     3781 cataaacaag agtatgagca ttgtatcccc ctgtatgttg atcccctaaa aataaaggat
     3841 tatccatata cccgccaaga gaaatagcat tctttaaaac agtttgaatg gaattataaa
     3901 ccgagaacag accatcttta ccaagagaat ctcctgtcac agtcggctta agctttgcct
     3961 caaagcaaat ttcatcggcg ttctatcttt gttatctgtt ttccctttac ttcctgaaca
     4021 aattccaaac ttggtatatt aattgacttc tccctgttaa tttcaacatc agtaatagtc
     4081 tttcctaaaa tctttttatc aagtagtttt cgatatgttt ccatttccgg aagctctggc
     4141 atagtgttat tctccttcat gttaaactgt aagtagtatt ggcatttaat gatttaatat
     4201 ttctaaaaga ttaaatcaaa tattaattac aaatgttttt acatattgcc ttattgtttg
     4261 gttacatgtc ggaaaacaaa aaagacacca accctccggt tggtgtccct cgtcaagtgg
     4321 ttcttacata ttgctgactt tttcctggct ctgtgcttct ttatatttta agcgagtggc
     4381 tttacctccc ctaatgtgtc tttcagactt atttttaagc aaaactttct ttacttcgtt
     4441 tgcagctgga gggttcagtt tgggaagacg ctctgttaca tccttatgaa ctgtgctttt
     4501 gctgacgccg aattttttag ccgtctgtct tacggtggat tgagtactta taatatattc
     4561 cgcaatctcc atggcccttc gttctatgta atctttcatt tttaaccccc tttggcatta
     4621 taccgtctta tttacatttt tatgcgtttc tttgtcctat tagaacctga ttttgttatt
     4681 caatggtaat atagtccaaa ggatttatat gttgcccgtc aacaattact tcaaaatgaa
     4741 gatgtgcacc atctgatgct tcaatgctga ctgtttttcc cacatttcca ataacctgac
     4801 ctttttcaac ggtatcaccg gctttgacat ttgccgacac taaattactg tacaatgttt
     4861 ttacattgtt tccatgatca ataataattt ccgttgctgt aatttctgaa ttatttacag
     4921 cttctacttt ccctgctaat gcggattcaa tcaataaggt atcttttggt ttgatatcaa
     4981 ttcccttatg tactctccat tcaccaatag cctcataata tactaattca tcaagcgtaa
     5041 attctcttat tatttctccc gctaatggtg acttcatgga ctttaaatct gttccttcag
     5101 gcttaactcc atcatcctca tcattgtcat cttctgcatt ttgaaattca tcaatgatat
     5161 cttgtacata atcttcataa ggatcttcat gaaccttaat atctccgttt ggctcattat
     5221 tttcagctaa atcactttga ccgttttcag gatttgaagt atatctccat actactgctg
     5281 ttgccaacag catgatacac aaagcaatta ttaagaagct tgtatcctta tgtcttagtc
     5341 taataaagaa atccttcatt ttttcttttg ctctcgcaaa tctattgttt ttcatataaa
     5401 cctcccattt gatacttctt tattatctgg aaatagtgtg tcccattttt tttatttaat
     5461 acaattttgt tctaaaaatt tctatttttc atagaatatt aaagaaactt atgataaagg
     5521 ggttggtttt tatgttaaac cgtataattt atgcttttgt tgtgttcaca atgcttttaa
     5581 ctattcctaa tctatctttg aatttctttg agaaaaaaat gaatttaacc agtgctgctg
     5641 tgcaacaaaa ggacagctca aagtatgcag atgaaaagca tgaagaaata tacactgcag
     5701 atgtatatga acctgaatta attaaggtgt acaacaacaa aacaaatgaa gtgatgatta
     5761 ttgattttga agaatactta aaaggagtag ttgcatcaga aatgccggca gaattcaata
     5821 tcgaagctct taaggctcag gcagtttctg caaggacata tttattgtat cgattaaaaa
     5881 aatatccgga tggacagccg gaacattcag aagcgcctgt ttgcacaggt gttcattgcc
     5941 aggtgtggaa ctcgaaggat gaattgatca ggtctcatga agaaggatgg tatgacgaaa
     6001 gctggggtaa aattgaagaa gcagttaatt caaccaaggg gcaaattttg gtttatgata
     6061 acaaaataat tgagccgctg tatcattcca ctagcggcgg cagaacagaa aactcggaag
     6121 atgtattttc tacggcagtt ccttatttaa gaagtgttga aagtccatat gaaggagatt
     6181 cgcccaaatt aaacggctcg attaaaatta caaccagtga attcattgac aaaattgaat
     6241 ctgcctacgg tgatatggat ataacagaaa gtaatttggc tgataaaatc gtgctcggcg
     6301 aagtcagcga agggggtaaa atcaagacat taataattga taacaccgaa atttcggggc
     6361 gtgatatgag ggcattattt aacttaaatt caacaaattt cagctttata caatcgggaa
     6421 atgaaattga aattttaact accgggtatg gtcacggcgt agggatgagc cagtggggtg
     6481 ccgaaggtat ggctaataga ggctataact acaagggaat attgaaacac tactatacgg
     6541 gtgttgaaat agtgagtatg a
//
LOCUS       DAOSOW010000057         6537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_11889_length_6537_cov_4.142857, whole genome shotgun sequence.
ACCESSION   DAOSOW010000057 DAOSOW010000000
VERSION     DAOSOW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6537
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11889_length_6537_cov_4.142857"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PLD67_02495"
     CDS             <1..315
                     /locus_tag="PLD67_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOT21428.1"
                     /translation="FWYTGCQFCVVEMPDLQKLQDTYQDDLLLLAINVGESKEKVEEF
                     LDENNLSFTVLLDEDMNVAYDYGIRSFPTTIAVNKKGEVIGGYIGMLTYEQMEQLYGF
                     FE"
     gene            complement(376..1401)
                     /gene="ltaE"
                     /locus_tag="PLD67_02500"
     CDS             complement(376..1401)
                     /gene="ltaE"
                     /locus_tag="PLD67_02500"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HOT21429.1"
                     /translation="MKFIDLRSDTVTMPTDEMRQAIANAEVGDDVYQDDPTVNKLEKI
                     AAEKVGKEAALFVPSGTFGNQLAIMTHTRRGNEVIVGKNNHIVVHEVGASAVISGVQL
                     RTLETVNGVMSPKDVLKAIRSDDIHEPETGLICVENAHGCGAVVPLSILKEIKEIAEN
                     HSIPVHMDGARIFNAAISSGVNAKEIAACCDSVMFCLSKGLAAPAGSMVAGSKEFIDR
                     ARKNRKLMGGGMRQIGILAAAGIIALEKMTDRLVEDHENAKYLAEELSKLPGVKVKRD
                     RLDIDMVFFEMGEDVIKESVLVKKFYEKNIKINGTEDGEYRFVTHIGVSKEDIDYVIN
                     CMKELIK"
     gene            complement(1452..2303)
                     /locus_tag="PLD67_02505"
     CDS             complement(1452..2303)
                     /locus_tag="PLD67_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOT21430.1"
                     /translation="MGILLKGKPVADSIVARVKDEAEELRRNNIIPKLKIIRVGERED
                     DLAYERAAVKRMKSANIDCEVLALPLNIDKETFEDEIKNVVNDNSVHGILVFRPLPKQ
                     LNEKDIRFIINPEKDIDCLNPINAAKILEGDESGFPPCTARAAMEILKYYNIDISGKE
                     ATVIGRSMVVGKPLSMMLLKENSTVTICHSKTENLPQVAKRADILIAAIGKSKMITKD
                     YIKENSTVIDVGINADDEGNITGDVNTEDCIEKAAFITPVPAGVGSVTTAVLADHVVK
                     ACRLLNK"
     gene            complement(2313..2942)
                     /locus_tag="PLD67_02510"
     CDS             complement(2313..2942)
                     /locus_tag="PLD67_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HOT21431.1"
                     /translation="MNLTELSCKDFSFELSSKKPVPGGGGAAALAGSLGVALNMMVAN
                     FSKGKKKFIQYEKQHEDILKRGEKLRIDLLNLVEKDAEYFEPLSKAYSMPATTAEEKA
                     KKENQMQKCLKDACRAPLETMELVYEGIKLHEEILDISSLTIISDIGVGVQMLKAALN
                     SAYLSVLINLKYINDDQYVNNLKKRTEVLLTDGNKKADEIFEKVLITLR"
     gene            complement(2971..3579)
                     /locus_tag="PLD67_02515"
     CDS             complement(2971..3579)
                     /locus_tag="PLD67_02515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016080339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT21432.1"
                     /translation="MFRQTIYETDRLYLRILKPNYFRQVLEYYKRNHNFLKEWEPKRP
                     RDFYTAAYQKSQLKYEYNMFKRNKLVRFWIFKKEDNKLIGNICLSNIVMGNFRSCYMG
                     YKLDNDEINKGYMTEAIRKVIRIMFEDFGLHRIEVNILPRNVRSLRVMEKLNFEKEGY
                     SKRYLEINGKWEDHVHFAIYQDSFEEQKCAETARKLIFESNI"
     gene            complement(3817..4479)
                     /locus_tag="PLD67_02520"
     CDS             complement(3817..4479)
                     /locus_tag="PLD67_02520"
                     /inference="COORDINATES: protein motif:HMM:NF039407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6320 domain-containing protein"
                     /protein_id="HOT21433.1"
                     /translation="MRYCNNCNVSVVGKRKLCPLCQDRLIGDKPQEEVFPKISFVYRE
                     YSSFFKIMLLVSIIIATVSVAVNILIPESGAWSFLILGGLGSVWASLINLINQRKNIP
                     KNIVYQVMTISVISLIWDLLTGWKGWSINYVIPSVCVFAMISMAIISKIRKLYIEDYI
                     LYIIIDALFGIVPIIFVIFGLLDVLYPSIICISTSIISLSTIIIFEEKSLIAEIKRRL
                     HV"
     gene            complement(4479..5765)
                     /locus_tag="PLD67_02525"
     CDS             complement(4479..5765)
                     /locus_tag="PLD67_02525"
                     /inference="COORDINATES: protein motif:HMM:NF018902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21434.1"
                     /translation="MKNKRISEWSKLDNAAKIFPANSKKSDTKVFRFACELFDTVEPK
                     VLQQALDITLEAFPLYLSVIKSGLFWHYFEKSHFKALVTEETLPPCSTLYDKNNKTLL
                     FRVMYYRKRISFEVYHALADGAGALQFFRRLIFHYITIKYKYALRDKSPVLNIESSKT
                     QRLDDSFKRYYKEEKILTKKKRIKAYKIKGAKIPEYRIRIIEGVIPVDEILPKVKENK
                     TSLTIFLAAIFMCAINEEIPSRLKRKPVVLSIPVNLRNFYSSQTARNFFGVINVDYNF
                     AEGSGDLEDVIEVLKKKFKENLKPEIMARRINKLSSLEHNYVLRVIPLAIKNLILRIA
                     YAIADRSYTSTLSNVGIINMPDDIKPFIYSFDIFVSTDKIQACVNSFENILRISFTSA
                     FVSTEIQRRFFKTLTDMGINVTIESNIIDEEQEVLL"
     gene            complement(5766..>6537)
                     /locus_tag="PLD67_02530"
     CDS             complement(5766..>6537)
                     /locus_tag="PLD67_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004611006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT21435.1"
                     /translation="QISSKNHNIPVRVYSPEANFDCPLLLFFHGGGWVAGSIESYNKI
                     CTNMARLTNHKVVSVDYRLAPENPFPAGLEDCYETAKAFIANDEINDKEVTLIGDSAG
                     GNLAAALSLLARDRKEFKVDKQILIYPSTYNDYTENSPFKSVIENGTDYLLTSKRIRD
                     YMDLYKTKEDDIYSPYFAPLLSRDFTKQPKTLIITAEYDPLRDEGEEYGRKLREAGNY
                     VEIYRIKDALHGFFALPPKFPQVKLCYDIINRFLCEVN"
BASE COUNT         1878 a         1317 c          964 g         2378 t
ORIGIN      
        1 ttctggtata caggatgtca attttgcgta gtggaaatgc cagatttgca aaaacttcag
       61 gatacctacc aagatgacct tttgttattg gcaataaatg taggtgagag caaagaaaaa
      121 gttgaagagt ttttggatga aaacaacctg agctttactg ttttattgga tgaggatatg
      181 aatgtagctt atgattacgg catccgctcc ttcccaacaa ctattgcagt aaacaaaaaa
      241 ggtgaagtta taggcggtta tatagggatg ctcacttatg agcaaatgga acagctgtat
      301 gggttttttg aataggaaca tctgtgccat actggtgcca ggcacctttt aaggcgcctg
      361 gcaccagtgg tttaatcatt taatcaattc tttcatacaa tttataacat aatcaatatc
      421 ttcctttgat actcctatat gtgttacaaa gcggtattca ccatcctcgg ttccatttat
      481 tttaatattt ttttcataaa actttttaac tagaacactt tcttttatta catcttctcc
      541 catttcaaaa aataccatat caatgtcaag cctatcccgt tttactttaa ctccgggcag
      601 ttttgaaagt tcttccgcta aatatttagc attttcatgg tcttctacaa gtctgtcagt
      661 cattttttcc aatgcgataa ttcctgctgc agccaatatt cctatttgcc tcataccgcc
      721 gcccataagt tttctgttct ttcttgccct gtctataaat tccttgcttc ctgcaaccat
      781 tgaacctgca ggtgcagcta atccctttga taagcagaac attacggaat cacagcatgc
      841 cgcaatttct tttgcattta ctcctgatga tattgcagca ttaaaaattc ttgccccatc
      901 catatgaacc gggattgaat gattttccgc aatttctttt atttctttca atattgaaag
      961 gggtactact gctccgcacc catgagcatt ttcaacacat atgagacctg tttccggctc
     1021 gtggatatca tcggacctta tagcttttaa aacatctttg ggagacataa caccgtttac
     1081 tgtttccaaa gttctaagct gcactcctga aataacagca gacgctccaa cttcatgcac
     1141 aacgatatga ttattttttc ctactattac ctcattgcct cgtctggtat gcgtcataat
     1201 tgccagttga tttccgaatg taccgcttgg tacgaacaag gctgcttctt tacccacctt
     1261 ttcagcggca atcttctcca atttattaac cgtcgggtca tcttggtaaa catcatctcc
     1321 aacttctgca tttgcaatag cctgtctcat ttcatcagta ggcattgtaa cagtatcgct
     1381 tcttaagtct ataaatttca tttttccacc ctctattttt atttttaata aggtatgtta
     1441 attaaccgtt attacttatt taataacctg catgctttta caacatggtc tgccagcacg
     1501 gcagttgtaa ccgagcccac ccctgcaggt acgggagtta taaatgctgc tttttcaatg
     1561 cagtcctccg tgttgacatc tccggttata ttcccttcat catcggcatt gattccaaca
     1621 tctattacgg ttgaattttc ctttatatag tcttttgtaa tcattttaga ctttccaatg
     1681 gctgctatca gaatatctgc tcttttggca acttgtggca ggttttctgt ttttgaatgg
     1741 catatggtta cggttgaatt ctctttaaga agcatcattg acaaaggttt ccctaccacc
     1801 attgacctcc ctattactgt tgcctccttg ccgctgatat ctatattata atatttgaga
     1861 atttccattg ctgcccttgc tgtgcatgga ggaaatcccg attcatctcc ttccaatatt
     1921 ttagcagcat ttataggatt aaggcaatca atatcttttt ccgggtttat tataaatctg
     1981 atatcttttt catttagctg cttgggaaga ggtctaaata ccaaaatacc gtgaacactg
     2041 ttatcattta caacattctt tatctcatcc tcaaaagttt ccttgtctat atttaagggc
     2101 aatgccaaaa cttcacagtc aatgtttgca cttttcattc tttttacagc tgctctttca
     2161 taagccaaat catcttctct ttcaccgaca cgaattattt tcaattttgg aataatatta
     2221 tttcttctaa gttcttccgc ttcatctttt actctggcta caatactgtc tgccaccggt
     2281 ttgcccttta aaagtattcc cattttgcct ccttaacgca aagtaattaa aactttttca
     2341 aatatttcat cagccttttt atttccgtcc gttaaaagaa cttctgttct tttttttaaa
     2401 ttatttacat attggtcatc atttatgtat ttcaaattaa ttaatacact taaatatgca
     2461 ctgttaagtg ctgcctttaa catctgaact ccaacaccta tatcactaat aattgttagg
     2521 gatgaaatat ccagtatttc ttcatgaagt ttaattcctt cataaactaa ctccatcgtt
     2581 tctaaaggag ctctgcatgc atccttaaga catttttgca tttggttttc ttttttagct
     2641 ttttcttcgg ctgtagttgc aggcatactg tatgcttttg aaagaggctc gaaatattca
     2701 gcgtcttttt ctaccaaatt caataagtct atgcgaagtt tttctcccct tttcaaaata
     2761 tcttcatgtt gtttttcata ttgaataaat ttttttttac cttttgaaaa atttgcaacc
     2821 atcatattga gggcaactcc caaagagccg gcaagtgctg ctgcccctcc gccaccgggc
     2881 accggctttt ttgatgacag ttcaaaagaa aaatctttgc atgataattc cgtcaaattc
     2941 ataaaatcac ctctcatata ttataaccaa ttatatatta ctttcaaaga ttaattttcg
     3001 cgcagtttct gcacattttt gttcttcgaa ggaatcttga tatattgcaa aatgaacatg
     3061 atcttcccat ttgccgttga tttccaaata tctctttgaa taaccttcct tctcaaagtt
     3121 cagtttttcc ataactctca atgaccttac attccgaggc agtatattta cttcaattct
     3181 gtgcaggcca aagtcctcaa acataattcg tataactttc ctgattgctt ctgtcatata
     3241 tcccttatta atttcatcat tgtccaactt gtagcccata taacaggacc taaagtttcc
     3301 cataactata ttgcttaaac aaatattgcc tattaatttg ttatcttcct ttttgaaaat
     3361 ccaaaatctt accagtttgt ttcttttaaa catattgtat tcatatttca gttggctttt
     3421 ttgatatgct gcggtataaa aatcccttgg tctttttggc tcccattcct ttaaaaaatt
     3481 atgattcctt ttatagtatt ccaagacttg tctgaaataa ttcggtttta aaattcttaa
     3541 atataatctg tctgtttcat atattgtttg tctaaacatc ttattttcct ttcatatgtg
     3601 tcatttaatt tcataatcct tcttatatag gcagcagatc actttatcat gagatccttc
     3661 gctatcgctc aggatgacaa agcgtattaa ggcgcaaccc ttgttattgc tattaataaa
     3721 ctacgtgtca ttctgatagc tttagctcga agaatctcat ttttttaacc atgagatact
     3781 tcgctactct catgatggaa aaaatattta gaagtattac acatgcagcc ttctttttat
     3841 ttccgctatc aaactttttt cttcaaaaat tataattgtt gacagtgaaa tgatgcttgt
     3901 tgatatacaa ataattgacg ggtacaaaac gtccagtaaa ccaaaaatta caaagattat
     3961 ggggactatc ccaaacaatg catctattat tatatataaa atataatctt caatatagag
     4021 ttttctgatt tttgaaatta tagccattga tatcatagca aacacgcata cggaaggtat
     4081 tacataatta attgaccaac ctttccaacc cgttaataaa tcccatatta aagatattac
     4141 agatatggtc ataacctggt atacaatgtt tttgggaata tttttgcgtt ggtttatgag
     4201 gttaataaga cttgcccaaa ctgagcccaa tcctcccaat attaaaaatg accacgctcc
     4261 gctttccggt atcaatatat taaccgcaac tgaaacagtg gcaattatta ttgaaactaa
     4321 gagcataatt ttgaaaaatg aagagtattc tctataaaca aatgagattt tcggaaaaac
     4381 ttcttcctgg ggtttgtcgc ctatcagccg gtcctgacac aaaggacaga gctttctttt
     4441 tccgactacg cttacattac agttgttaca atatctcatt aaagcaacac ctcctgttct
     4501 tcatcaatta tattggactc aatagtaacg tttattccca tatcagttaa tgttttaaaa
     4561 aaccttcttt gtatttcggt acttacaaat gccgaagtaa aactgattct tagtatattt
     4621 tcaaaagagt ttacgcaagc ttgtattttg tcagtactta caaatatatc aaatgaatat
     4681 atgaaaggtt taatatcatc aggcatattg ataataccaa cattggacaa tgttgaagta
     4741 tagcttctgt ctgctattgc atatgcaatc cttaaaatca gatttttaat tgccagcgga
     4801 atcactctta aaacataatt atgctctaag gatgataact tgttaattct tcttgccata
     4861 atttcgggct ttaaattttc tttaaatttt ttctttaaaa cttctattac atcttctaaa
     4921 tcaccgcttc cctcagcaaa attatagtca acgtttatca ctccaaaaaa attacgggct
     4981 gtttgtgagg aataaaagtt tctaagattt acgggaatgc ttaatactac gggctttctt
     5041 tttaatcttg atgggatttc ttcatttatg gcacacataa aaattgcagc cagaaaaatt
     5101 gtcaaagaag ttttattttc cttaacctta ggcaatattt catcaacagg aataactcct
     5161 tcaattattc tgatacggta ttcgggtatt ttcgcaccct ttattttata tgctttaatt
     5221 ctcttttttt tagttaaaat tttttcttct ttatagtatc ttttaaaact gtcatccagt
     5281 ctctgtgttt ttgaagattc tatatttaaa acaggcgatt tatctcttaa ggcatattta
     5341 tatttaatag ttatgtagtg aaaaattaac cttctgaaaa actgcaaggc tcctgctccg
     5401 tccgctagtg catgatatac ttcaaaactt atgcgcttcc tgtaatacat aaccctaaac
     5461 agcaatgttt tattgttttt atcatataat gtggagcagg gaggtaaagt ttcttctgta
     5521 acaagggcct taaaatgact tttttcaaaa taatgccaaa aaagcccgct ctttattact
     5581 gacagataaa gagggaatgc ttccaaagta atatcaagtg cctgttgaag caccttgggc
     5641 tctaccgtat caaataattc acatgcaaac ctgaatactt ttgtatcgct ttttttgctg
     5701 tttgcaggga atattttagc tgcattatct aatttgctcc attcggatat tcttttattt
     5761 ttcaatcaat tcacctcgca aagaaaccgg ttgattatat catagcataa tttaacctga
     5821 ggaaatttag gaggaagagc aaaaaatccg tgaagggcat cttttattct gtatatttca
     5881 acataatttc ctgcttccct aagttttctg ccgtactctt caccttcatc acgcaagggg
     5941 tcatattctg cagttataat taatgtcttc ggctgctttg taaagtccct ggaaagcaaa
     6001 ggtgcaaaat aggggctata tatatcatct tccttggttt tatacaaatc catatagtct
     6061 cttattctct tggaagtaag caaataatcg gttccgtttt ctataacgga cttgaaggga
     6121 gaattttccg tgtagtcatt gtatgttgac ggatatatca atatttgttt atcaacctta
     6181 aattcttttc tatcccttgc aagcaaggat aaagctgcag ccaaattgcc tcctgcacta
     6241 tctcctatca aagttacttc tttatcatta atttcatcat ttgcaataaa tgcttttgct
     6301 gtttcataac aatcctctaa tcctgcggga aagggattct ccggtgccag cctgtaatcg
     6361 acggacacaa ccttatggtt tgtcaacctt gccatatttg tacaaatttt gttgtaactt
     6421 tctatactgc ctgccaccca tccgccgcca tgaaaaaata aaagcaaagg acagtcgaaa
     6481 ttcgcttcgg gagaatatac ccgaacagga atattgtgat tttttgagct tatttga
//
LOCUS       DAOSOW010000058         6420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_12183_length_6420_cov_3.758995, whole genome shotgun sequence.
ACCESSION   DAOSOW010000058 DAOSOW010000000
VERSION     DAOSOW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6420
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12183_length_6420_cov_3.758995"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PLD67_02535"
     CDS             <1..330
                     /locus_tag="PLD67_02535"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOT21436.1"
                     /translation="PIALMTLGGEIEINNIWKNIKPLVFVVAGKLIIFPAIATLIAIS
                     YGYRGMELGVILSMTAPPTAVTSYALAQQHNCNYELAGQIVFLTTLISPFTIFLFVYI
                     FKAAGLF"
     gene            343..1281
                     /locus_tag="PLD67_02540"
     CDS             343..1281
                     /locus_tag="PLD67_02540"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOT21437.1"
                     /translation="MDNFIFSLNVVLPLVFLMFIGSFLRRINIFDEDFLRKLNKITYC
                     VLLPVLLFNNIYTSKVSGEIDLEFIIFFVILALAVIGILLIVIPKYEKDNRNRGVLIQ
                     GLFRGQFVFFGIPLMANIFGDEGLVVLSTLIAIIVPIYNFLAVIILDLFTDERHNYKA
                     TIISILTNPLVIGSLLGIAASVLQIKLPSALNKTIADISKTVTPISLMILGGVIEINN
                     IWKNRKKLILIAAVKLFIIPSFAALITIFFGYRGIELGAVLSMIAPPTAVASYTLAQQ
                     HDCNDELAGQIVFFTTLISPFSIFLFIFVFKTFGLF"
     gene            1423..1779
                     /locus_tag="PLD67_02545"
     CDS             1423..1779
                     /locus_tag="PLD67_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21438.1"
                     /translation="MMVIKKFSFSNKNNDEKLLNWEDSVKLDSQQKLREQMKQMNNEN
                     KNRNKNDNRFDNRNDNKFDNRNENKFDNRNDNRNDNNKFDSRNDDDWKSLNKNNQDNK
                     NNNKNKNKNSQNNNLK"
     gene            2004..3104
                     /gene="gcvT"
                     /locus_tag="PLD67_02550"
     CDS             2004..3104
                     /gene="gcvT"
                     /locus_tag="PLD67_02550"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOT21439.1"
                     /translation="MKKTPLYEKHIQLKGKMIDFGGWSLPVEYTGIIPEHEAVRTKAG
                     IFDVSHMGEITVKGEDSEKYLQMLLTNDISLLNNNQIAYTAMCYPHGGVVDDLLVYKY
                     SNTDYLLVVNASNTEKDYQWMKENIFGNTEILNVSENYAQLALQGPLAETILQKLTSK
                     NLNEIEFYYFSDNVKVGNIEALVSRTGYTGEDGFELYFAYDKAEEMWDLILEAGKDEG
                     LIPAGLGARDTLRFEASLPLYGHELNENITPLEAGLGFFVKLNKENFIGKEALARQKS
                     EGLKRRIVGFEMIDRGIPRNNYEVYKEGEKIGYVTTGSFSPTLKKNIGLALIDSAYSK
                     EGTEIEILIRNKNSKAKVIKKPFYSKKYKKMA"
     gene            3189..3563
                     /gene="gcvH"
                     /locus_tag="PLD67_02555"
     CDS             3189..3563
                     /gene="gcvH"
                     /locus_tag="PLD67_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOT21440.1"
                     /translation="MKLLPELKYVESHEWVRVEENKAYVGITDYAQEQLGDIVYVEVP
                     EVGTVVEAGDQFVVLESVKAASDVYAPVSGTVVEVNEELEDNPALINESAYDAWIVAI
                     DMSMPTEVDSLLSAEEYEELCE"
     gene            3574..4911
                     /gene="gcvPA"
                     /locus_tag="PLD67_02560"
     CDS             3574..4911
                     /gene="gcvPA"
                     /locus_tag="PLD67_02560"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOT21441.1"
                     /translation="MTRYIGNTTDDRQQMLNEIGYKDIDSLFRAVPDSVRLKEKLKIP
                     EALSEMELIKKIKSLSDKNFNSDEYVCFMGAGAYDHYIPSAIDQLIMRQEFYTAYTPY
                     QPEISQGTLQAIFEYQTMISELTGLPVANASMYDGATALAEASAMSCEATRRKEVLVA
                     SSVHPESRRTLNTYAKFRNITVKEISYENGQISLEELNTKINDNTAAVIIQNPNFFGI
                     IEDVEKIAEITHKNKSLLVLSADPISLSILKSPGEMGADIAVGEGQPLGNSLNFGGPY
                     LGFMAVTEKLMRKMPGRIVGQTTDKEGRRGFVLTLQTREQHIRREKATSNICSNQALN
                     ALQATMYLTFMGKEGLKKVAKLCLNKSHYAYSELIKTNKFKPLFTAPFFKEFAVISDK
                     PVSDLNEKLLKSNIIGGYNIEIDYPELRNAYLLAVTEKRTKEEIDALVRKAGE"
     gene            4913..6361
                     /gene="gcvPB"
                     /locus_tag="PLD67_02565"
     CDS             4913..6361
                     /gene="gcvPB"
                     /locus_tag="PLD67_02565"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOT21442.1"
                     /translation="MQLIFEKSKKGRKAYSLPECDVPDVSLDVIDKKYLRAEAPELPE
                     VSELDAVRHFTQISQRNHGVDSGFYPLGSCTMKYNPKVNELAARFDGFTKIHPYQQEK
                     TVQGALKLMYDLGRMLSEITAMEEFTLQPAAGAHGELTGLMIMKAYHHNRNNEKRTKI
                     IVPDSAHGTNPASAAVAGFDVVEIKSNDKGLVDLEELKAAVGEDTAGFMLTNPNTLGL
                     FDENILEISKIVHDAGGLMYYDGANANAILGISRPGDMGFDVVHLNLHKTFSTPHGGG
                     GPGSGPVGVKKHLAEFLPVPVISKKEEEYYMDYERPNSIGKVKSFYGNFGVLVRAYAY
                     ILSMGPDGLKEVAENAVLNANYIANKLKDKYFLPIDTECMHECVFSADWQKEKNGVSA
                     LDIAKRLLDFGFHPPTIYFPLIVHEALMVEPTETESKETLDEFISVMRQIAEEAENTP
                     EMVKTAPHTTVISRLDEATAARKPILRYTRLT"
BASE COUNT         2363 a          974 c         1267 g         1816 t
ORIGIN      
        1 cccatagcat tgatgaccct tggcggagaa atagagataa acaatatatg gaagaatatt
       61 aaacccttag tttttgttgt agcgggaaaa ctaattattt ttcctgcaat tgcaaccttg
      121 attgcaattt cctacggata cagagggatg gaattaggtg taatattaag catgacagca
      181 cctcctacgg ctgtcacaag ctatgccttg gcacagcagc acaactgcaa ttatgaattg
      241 gcaggacaaa tagtattttt aactacatta atcagcccat tcactatttt cttgtttgta
      301 tacattttta aagcagcagg attattttaa agaggtcaaa agatggataa ttttatattt
      361 tctttaaatg ttgttttacc attggttttt ttgatgttta taggttcctt tcttcgaaga
      421 attaatatat ttgatgagga ctttttaagg aaattaaata aaattacata ttgtgtacta
      481 ttaccggttc ttctttttaa caacatatat acaagcaagg tatcagggga aattgactta
      541 gaatttatta ttttttttgt tattttagct ttggctgtta ttggaatatt attgatagta
      601 atccccaagt acgaaaaaga taacagaaac agaggagttt taattcaggg tctgtttaga
      661 gggcaatttg tgttttttgg aatacccctt atggccaata tttttggaga tgaaggattg
      721 gttgttctat caaccctcat cgcaataata gtgccaattt ataatttctt ggctgttatt
      781 attttggatt tatttacgga tgaaaggcat aattataaag ctaccataat aagcatttta
      841 accaatcctt tagtaatcgg ctccttgctt ggaattgcag catctgttct tcaaataaaa
      901 cttccctccg cccttaataa aacaattgct gatatctcaa aaacggtaac acccatatca
      961 ttaatgattc tcggcggagt gatagaaata aacaatatat ggaaaaacag aaaaaagtta
     1021 attcttatag cagcagtaaa attatttatt atcccttcat ttgcagcatt gattaccata
     1081 ttcttcggat acagaggaat tgaattagga gcagtattaa gcatgatagc tccccctacg
     1141 gctgtggcaa gctatacttt ggcacagcag catgattgca atgatgaatt ggcaggacaa
     1201 atagtatttt ttacaacatt gatcagcccc ttcagtattt tcttgtttat ttttgttttt
     1261 aaaacttttg gtttgtttta gtaattttat tattgcagtt aaaaaattga atttatggat
     1321 accaacccca cattaagtat ttattggaat aataattaat atgaaaaagc taataatatt
     1381 catgtagatg acaaacatct tcaaatacat gcatatgaag ggatgatggt aataaagaaa
     1441 ttcagtttta gcaataagaa taatgacgaa aagttattaa attgggaaga cagtgtcaaa
     1501 ttagacagcc aacaaaaatt aagggagcag atgaaacaaa tgaataacga aaacaaaaac
     1561 agaaacaaaa atgacaacag atttgacaat cgaaacgaca acaaattcga caatcgaaat
     1621 gagaacaaat tcgacaatcg aaacgacaat cgaaacgaca acaacaaatt tgacagtaga
     1681 aatgatgacg actggaaaag tctgaacaaa aacaaccagg ataataaaaa caacaataaa
     1741 aacaaaaata aaaacagtca gaacaataat ttaaaatagt tgggtatttg aaacggtcat
     1801 tctcaatgac cgttttttaa taccggtaga tgaaagtaat atgcatataa aagtattttt
     1861 tttcacaaac aatatatatt gacacggata tttattggtt ttataatatt gacaaataat
     1921 tttaataaag tgataatgaa taaaaaataa tatcaaaaac aaagacagat attttctgtc
     1981 tttaatttta agaggagtgt gtaatgaaaa aaacgccttt atatgaaaaa catattcagc
     2041 ttaagggaaa gatgattgat tttggcgggt ggagcctgcc ggttgaatat acaggaataa
     2101 ttcccgagca tgaagctgta agaacaaaag caggaatttt tgatgtatct cacatgggtg
     2161 aaataacagt taaaggagaa gattccgaaa aatatttgca aatgttatta actaatgata
     2221 tttcattgct taataacaat caaattgcct ataccgcaat gtgttatcct cacggcggag
     2281 tagtagacga tttgttagtc tataaataca gcaatacaga ttatctattg gttgttaatg
     2341 catccaacac tgaaaaggat tatcagtgga tgaaagaaaa catatttgga aatactgaaa
     2401 tattaaatgt ttcagaaaat tatgcccagc ttgctctgca aggacctctg gctgaaacaa
     2461 ttttgcaaaa gttgacaagt aaaaatctaa atgaaataga attctattat ttcagcgata
     2521 atgtaaaagt aggcaatatt gaagcattgg tttccaggac cggttatacg ggagaagatg
     2581 gttttgagtt atattttgct tatgataagg ctgaagaaat gtgggatttg attttagaag
     2641 caggaaaaga cgaaggcttg ataccggcag gattaggcgc aagagataca ttaagatttg
     2701 aagcatcact gcccctttat ggccacgaat taaatgaaaa tattacgccc ttagaagcag
     2761 gacttggttt ttttgtaaag ctgaacaaag aaaatttcat aggaaaggaa gccttagcta
     2821 gacaaaaatc agaaggtctt aaaagaagaa tagtcggttt tgaaatgatt gacagaggaa
     2881 taccgagaaa caactatgaa gtatataaag aaggggaaaa aataggatat gtaacaacag
     2941 gaagtttttc acctacctta aagaaaaaca taggattagc cctcatagat tctgcatatt
     3001 caaaggaagg aacagaaata gaaattttaa taagaaataa aaattctaag gcaaaagtta
     3061 ttaaaaagcc tttttattcc aaaaaatata aaaagatggc ctaaccctat tctatacata
     3121 cgaaaaaatt taattaaaaa tatatttatt gggtatctat aataatatct aaatacacgg
     3181 aggaaattat gaaattacta ccagaattaa aatacgttga atctcatgaa tgggtgagag
     3241 tagaagaaaa taaggcatat gtaggaatta ctgactatgc acaagaacag ttaggggata
     3301 ttgtttatgt ggaagtgcct gaggttggaa ctgtagttga agcaggcgac cagtttgttg
     3361 tacttgaatc agttaaggcg gcttcagatg tatatgcccc cgtttcagga acagttgtag
     3421 aagtaaatga agagttggaa gacaatccgg ccttaatcaa tgaatctgca tatgatgcat
     3481 ggattgtagc aatagatatg agcatgccta ctgaagttga cagcttgtta tcagctgaag
     3541 aatacgaaga attatgtgaa taagggagca gttatgacaa ggtatattgg aaacaccact
     3601 gatgacaggc agcaaatgct aaatgaaatc ggatataagg atatagattc tctgttcaga
     3661 gctgttcctg attcggtacg attaaaagaa aagttaaaaa tacctgaagc tttgtcagaa
     3721 atggaactga taaaaaaaat aaaatcatta tccgataaaa attttaatag cgatgaatat
     3781 gtatgtttta tgggtgccgg cgcatatgac cattacatac cttcagcaat agatcagtta
     3841 ataatgaggc aggaattcta tacagcatat actccctacc agcctgaaat aagtcaggga
     3901 acattgcagg caatatttga ataccaaaca atgataagtg aattaaccgg acttcccgtt
     3961 gctaatgcat caatgtatga cggtgcgaca gctttggcgg aagctagtgc aatgtcttgt
     4021 gaagcaacac gcaggaagga agtattagta gcatcttctg tccatcccga aagtaggcga
     4081 actttaaata catatgctaa attcagaaat ataacggtaa aagaaatatc atatgaaaac
     4141 ggtcaaataa gcttagaaga gctaaataca aagattaatg ataatactgc agccgtaatt
     4201 atacaaaacc cgaatttttt cggcataatt gaagatgtag aaaaaattgc cgaaataact
     4261 cataaaaaca aaagtctttt ggtcttaagc gcagatccta tttcattgtc aattttaaaa
     4321 agtccgggag aaatgggagc ggatattgct gtgggtgaag ggcagccgct tggaaattca
     4381 cttaatttcg gaggacctta tttaggcttc atggctgtta cagaaaagtt gatgcgaaaa
     4441 atgcccggaa gaattgtagg acagacgacc gataaagaag gcaggagagg attcgtactg
     4501 actcttcaaa caagggaaca gcatataaga agagaaaaag caacctcaaa tatatgctct
     4561 aaccaagctt taaatgcact gcaggcaacc atgtatttaa catttatggg aaaggaagga
     4621 ctaaaaaaag ttgcaaagct ttgtcttaac aaatcccact atgcttatag cgagttaatt
     4681 aaaaccaata aatttaagcc cttgtttaca gcaccattct ttaaagaatt tgctgtaata
     4741 agcgataaac ctgtaagcga cttaaatgaa aaactcttaa aaagcaatat aataggcggc
     4801 tataatattg agattgatta tcctgaatta agaaatgctt acctgcttgc ggtaaccgaa
     4861 aagagaacca aagaagaaat agatgcatta gtaagaaagg caggtgagta atatgcaatt
     4921 aatatttgaa aaaagtaaaa aaggcagaaa ggcatattca cttcctgaat gcgatgtgcc
     4981 ggatgtatcc ttagatgtga tagataaaaa atacttacga gctgaggctc ctgagcttcc
     5041 tgaagtcagc gagcttgatg cagttcgtca ctttacacaa atttctcaaa gaaatcatgg
     5101 tgtagattcc ggattctatc cgctcggaag ctgtaccatg aaatataatc ccaaagtaaa
     5161 cgaattggcg gctagatttg acgggtttac aaaaattcat ccgtatcagc aggaaaaaac
     5221 agtacagggt gcattaaaat taatgtatga cctgggcaga atgctttctg aaataacggc
     5281 tatggaagaa tttactctcc agccggcggc aggagctcat ggagagctga cgggattaat
     5341 gataatgaaa gcttatcatc ataacagaaa taatgaaaaa agaaccaaaa taatagttcc
     5401 ggattcagca cacggtacaa atcccgcttc tgcggcagtt gccggatttg atgtagttga
     5461 aataaaatct aatgacaagg gcttggtaga tttagaagaa ttgaaagctg cagtcggaga
     5521 agacacagca ggattcatgc tgactaatcc caatacatta ggtttatttg acgaaaatat
     5581 attggaaata tctaaaatag tccacgatgc cggcggcttg atgtattatg acggagccaa
     5641 tgcaaatgca attttaggca tatcaaggcc gggagatatg ggatttgatg tggtgcactt
     5701 aaacctccat aaaacattca gtacacctca tggaggcggc ggtccgggtt caggtcctgt
     5761 aggagtgaaa aaacatcttg cggaatttct gccggtaccg gttatttcta aaaaagaaga
     5821 agagtattac atggattatg aaagaccaaa ttcaatcggc aaagtaaaat cattttacgg
     5881 taattttggt gtattggtaa gagcatatgc atatatttta agcatgggtc cggatggact
     5941 taaggaagta gctgaaaatg cagttttaaa tgcaaattat attgcaaata agcttaagga
     6001 caaatacttc ctgccaattg acacggaatg catgcatgaa tgtgtgtttt cagcggattg
     6061 gcagaaggag aagaatggag tttcagctct cgatatagca aaaagattgc tggatttcgg
     6121 atttcatcct ccaacgattt acttcccctt aattgttcat gaagcattga tggttgaacc
     6181 cacggaaacc gagtcaaaag aaacactgga tgaattcatt tctgtcatga gacagatagc
     6241 agaagaagca gaaaatactc cggagatggt aaaaacagct ccccacacaa cagttataag
     6301 caggttagat gaagcaacag ctgcaagaaa acccatactt cgatatacga ggttaacata
     6361 attaccaatt atgtaaactg ggaaggacaa tattgtctct tcctttttat tgacaccata
//
LOCUS       DAOSOW010000059         6364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_12325_length_6364_cov_3.570455, whole genome shotgun sequence.
ACCESSION   DAOSOW010000059 DAOSOW010000000
VERSION     DAOSOW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6364
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12325_length_6364_cov_3.570455"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="PLD67_02570"
     CDS             <1..1467
                     /locus_tag="PLD67_02570"
                     /inference="COORDINATES: protein motif:HMM:NF040368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6273 domain-containing protein"
                     /protein_id="HOT21443.1"
                     /translation="RGITVSESTDGNITIALSRDVNTDAIEFIAIKGPVTLITQKLIQ
                     ATIKEGTTIIVKSSIDGKEIEFTGTGEEVEILDGYPIVPVTINYLDEKTKEVIASVIE
                     YSIDNGENWIAGPGSELDLIPGKDVIFRIAEMPSRELQTLIVPERPEGISFSYADSVS
                     DYLMGLPAGATNIEARILAGERVYQDWTDITVDSDGKASLIHGGGDLIQVRTKAVTGS
                     AFAGDIVQHYSKKSISELDIGDLVVDNSWQWEYKTGSGYSGTGELKPVTWIVAAKEHY
                     GMDSGVTLLSKDLIGQFTFDNRTDVHHNGVNLWGPSNHIRPWLNSVGIYEENGFYNAF
                     SIEFMNSVLMTDVPNEGYWEGKYTTQDKVFIPSITELGAPEVALEYYDEELDETFEIG
                     ITYVIGTVYPYFENASNSDRIAKLGASNFSYWTRSPEDWLKDIVHCILNDGSRGIYWG
                     ENLQAKDNNGVRPAVNIAPDTLISGIPNENGAYEIFYE"
     gene            complement(1591..2994)
                     /locus_tag="PLD67_02575"
     CDS             complement(1591..2994)
                     /locus_tag="PLD67_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016114456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOT21444.1"
                     /translation="MKKVNVSLRPIVSNINLPTVIKTAILPGDTMESIFVATQVGEIF
                     YIRNRIARLFLDIRQRIIELGTNGGYDERGLLGLAFHPNFYYNGLFYLHYSKAGTQGQ
                     GALSGSFHPNPCEPETLNLRWVNRNTQYDHIDTVEEWILQPSGQSQRRRTLLNLRRPF
                     LNHNGVNNLNFSPETGRLVLTTGDGGSGYDPFNLSQNIMEIAGKIIEIDVNTDILINN
                     LPAVTRFNELPEAVQRTLTVIAKGTRNIPGIAFQRYNNQYIKYVGNVGQDLVESVFSF
                     IYYKPIPVTEIISASTQNRSLNEDGLINLGWRGWEGDLPTPIINPCLSNPSLVEETIA
                     YYNEAISLATKRILPLTCYYHMDSRPDKFSGTALTGIQPYMGNEIPDLTGCIVFIDFV
                     RRGSTPARGVLAYTKVRTECKLNDYSIIEPNYNFGTQSAYYVSLGANLTQSRLYLGVY
                     GSSNVTDFNQGTVFEIV"
     gene            complement(3104..3283)
                     /locus_tag="PLD67_02580"
     CDS             complement(3104..3283)
                     /locus_tag="PLD67_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21445.1"
                     /translation="MLNGRTYINRKVNKLESSGNTGFLEVRVFSTTTSFNVVLTPVME
                     GESYVRFIIIPEKHW"
     gene            3426..3953
                     /locus_tag="PLD67_02585"
     CDS             3426..3953
                     /locus_tag="PLD67_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT21446.1"
                     /translation="MVAEELLILNLKRGREEAYRQLIEKYGNRLYRTCYLILNDREEA
                     EDVVQETFIKVFKKIDTFKGQSGLYTWIYAIALNLSRDRIRMKQDMLELKDEWIGSYD
                     VESLVEINIDRELLKKEIFEMNSLYREVLVLFYFEELSIKEISNLLNEKEGTIKSKLS
                     RGRNILKESLLKGGR"
     gene            3953..4264
                     /locus_tag="PLD67_02590"
     CDS             3953..4264
                     /locus_tag="PLD67_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21447.1"
                     /translation="MDKKDKLGIEIKSIMEEETLDLTLSQKAFDNIIQHRKKSLREKI
                     QEFINKEIEIPVAPAIIGFAALLAVTLIPMDIFKSQNERIIDMGGSYVIIREGFEVSK
                     K"
     gene            4261..>6364
                     /locus_tag="PLD67_02595"
     CDS             4261..>6364
                     /locus_tag="PLD67_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21448.1"
                     /translation="MKIKIKIKTLIFAVFILTLTFIWAIPTATLMIAGYLETKDSEKA
                     SLFYEKYASYPTTPSIESKYLHANSLIRSFNKFTIFLTGWGGGENTSPEDIEKAKQIL
                     METLAEKPVKNREKDYYIKSYKMLLDILIASGDAETLQEWIAFGQKGTDERLVYTADV
                     YDGFLHHVNGEGEAAKSIIEKYDETELKDTIFYVLKAEIALFDGKYEESEKIYDNIYK
                     NNWRELGKSFGSGGYYRNDWFDRFMEDFKGDNVITGTVTCEGKPMPFVEIYVQEAKEG
                     FSFYASGENYVAITDVNGEFKTLGLKDGLYSVGIGVDGSVLANKAFQGSTMQYVELNG
                     EDGEFHLSFRNTLKIYSPEPGEKLSGEEFTVSWEEIEDAAYYKVEPVVFYEPFGERGI
                     STRIYAKDKNGEYKFTENQAVFNINMIRNNPYITVSDSDNGYLGAQAVLGIFIPGVEY
                     PIVVNAYDENNNLITGSLPLRSYYEHIPSITAEGSLNEGENFIYRKNYPAAIEYYENI
                     LKENPDDIDALRYLTRIYGIGWKEGEQNFERAFTLGQRYTEISGNRELLTNTLGGMNI
                     SEIKENRENVYLTLKEAMKEPDFDNYYFLSRYYIAVENYEGAREALQMAENVTDNLIF
                     LNMYFGDYKEAVESIKSERFYRSRLSSNKVKDAIEALDESPPRGDELKVFNDFLLKLI
                     NGIPYGETRNIYNKTVKQI"
BASE COUNT         2219 a          964 c         1303 g         1878 t
ORIGIN      
        1 aggggtatta cagtaagcga atcaacagat ggaaatataa caattgcttt aagcagagat
       61 gtcaatacgg atgctataga atttatagca atcaagggac ctgttacatt aataacgcaa
      121 aagcttatac aagcaactat taaggaagga acaacaatca ttgtcaaatc cagtattgat
      181 ggaaaagaaa ttgaatttac aggtaccggc gaggaagttg aaatattaga cggttatccc
      241 atagttccgg ttacaattaa ttatttagat gaaaagacta aggaagttat agcttcagtt
      301 attgagtata gcattgataa tggagaaaac tggatagccg gacccggcag tgaattggat
      361 ttgatacccg gaaaggatgt tatttttaga attgctgaga tgccgtcgag ggaattacaa
      421 acacttatag taccggaacg gccagaaggt atatcatttt cctatgctga tagtgtcagt
      481 gattatttaa tggggttgcc tgcaggcgca actaatatag aagcaagaat tttagccggc
      541 gaaagggttt atcaggattg gactgatata acagttgact ctgatggaaa ggcaagttta
      601 attcacggtg ggggagattt aattcaagtc aggacaaagg ctgtaaccgg gtccgctttt
      661 gccggagata ttgttcagca ttattccaaa aaaagtatca gtgaattaga tattggagat
      721 ttggttgtag ataattcttg gcagtgggag tataaaaccg gcagcggata ttcgggaacc
      781 ggtgaactaa aaccggttac ttggattgta gcagcaaaag aacattacgg catggattcc
      841 ggagtgacct tgttgtctaa agatttaata ggtcaattta cctttgacaa taggactgat
      901 gtacatcata acggagttaa tctttgggga cccagcaacc atatccgccc atggctgaat
      961 agtgtgggga tatatgagga aaatggattt tacaatgctt tttcaataga atttatgaat
     1021 tcggtattaa tgactgatgt accaaatgaa ggttattggg aaggcaaata cacaacacaa
     1081 gataaagtgt ttattccatc aattacagaa ttgggtgctc cagaggtggc tttggaatat
     1141 tatgatgaag aacttgatga aacttttgaa ataggaatta cttacgtaat aggtacagtt
     1201 tatccatatt ttgaaaatgc atccaattca gatagaattg ctaaattagg agcaagcaat
     1261 tttagctact ggacccggtc tccggaagat tggctgaaag atatagtgca ttgcatctta
     1321 aatgacggaa gtcgtggaat ttattggggt gaaaatctac aagcaaaaga taataatggt
     1381 gttcgacctg ctgtaaatat agcacctgat acactgatat cgggtatccc gaatgaaaat
     1441 ggggcttacg agattttcta tgaataggtt gactgcagca agataaatta agattataaa
     1501 tagaaaaaaa ggccagttgg attatttatc tatactggtc ttttattatt atatgaatac
     1561 tgattaacat ttaatcaatt catgattagg ttatacaatt tcaaaaacag ttccctgatt
     1621 aaaatcagtt acattggatg agccgtatac acctaaatat agtcttgatt gagtgaggtt
     1681 tgctcccaaa cttacatagt aagctgattg agttccaaaa ttataattgg gttcaataat
     1741 gctgtaatca ttcagtttgc attccgtccg cacttttgtg taagccaata ctcctcttgc
     1801 aggtgtgctt cctctcctga caaaatctat aaaaacaatg cagcctgtca aatcgggaat
     1861 ttcattaccc atatagggct gaatacctgt aagagccgta ccgctgaatt tatcgggacg
     1921 cgaatccatg tgataataac aggtcaaagg taatatgcgt ttggttgcta aactgattgc
     1981 ttcattgtaa taagctatgg tttcttcaac caaagaagga tttgataaac aggggtttat
     2041 aataggtgtc ggcaaatcac cttcccatcc tctccaaccg agattgatta aaccatcctc
     2101 attaagacta cgattttgag tagaagctga aataatttct gttaccggaa taggtttata
     2161 ataaataaaa gaaaaaaccg attctaccaa atcctgaccg acatttccga catatttaat
     2221 atattgatta ttataccttt gaaatgcaat gcccggaata ttgcgggtac cttttgcgat
     2281 tacggtaagc gttctttgaa cagcttcggg aagctcatta aaacgtgtaa cagccggaag
     2341 attgtttatc agtatatcag tgtttacatc aatttcaata atttttcctg caatttccat
     2401 tatattttgg cttaaattaa aagggtcata acctgagccg ccatccccgg ttgtaagtac
     2461 taaccttccc gtttcaggtg aaaagtttaa gttgttgaca ccattatggt tcaaaaaggg
     2521 tcttcttaaa ttcagcagtg tacgccttct ttgagattga ccgcttggtt gtaaaatcca
     2581 ctcttcaacc gtatcaatat ggtcatattg cgtatttctg tttacccatc ttaagttcaa
     2641 tgtttccggc tcacagggat ttggatgaaa tgagcctgaa agagcaccct gtccttgtgt
     2701 tcctgctttt gaataatgaa gataaaaaag cccgttgtaa taaaagttag ggtgaaaggc
     2761 taagcctaaa agcccgcgct catcatatcc gccattggtc cccaactcaa taattctctg
     2821 gcgaatatcc aaaaaaagcc ttgctattct gtttctgatg tagaaaatct cacctacctg
     2881 agtagcaaca aatatgctct ccattgtgtc acccggcagt atggcggtct ttataacggt
     2941 tggtaaattt atattgctga caattggtct taaactaaca ttaacctttt tcaattattt
     3001 tcactccttt atattttttg gagggggtat ctgcctcgtt taaatatatg tttacatact
     3061 ttttattatg caggcatatc cctgatgtaa ttaaaaatgt taattaccaa tgtttttcag
     3121 gaattataat aaatcttacg taagattctc cttccataac aggagttaaa acaacattaa
     3181 aagaggttgt tgtagagaat accctcacct ctaaaaaacc tgtatttcca cttgattcaa
     3241 gtttattgac ttttctattt atatatgtcc tcccattaag catttataac atcctttcat
     3301 tatttttcaa tcattagtat aatatattca ataagaaatc tttgtaaaca gaatacatta
     3361 tttatttcaa ataaatgttt gcaacctttt gttcatttca tcgtcaaaaa tgttgggaga
     3421 gtgttatggt agcagaagaa ttattgatat tgaacttaaa aagaggtcgt gaagaggctt
     3481 acagacaatt aatagaaaaa tatggcaata gattgtatag aacctgctac ctgatactaa
     3541 acgacagaga ggaagcagaa gatgttgttc aagaaacatt tataaaggta tttaagaaaa
     3601 ttgatacatt taagggacag tcagggcttt atacatggat ttatgcaatt gctctcaacc
     3661 tctcaagaga cagaataagg atgaaacagg atatgctcga gttgaaagat gagtggattg
     3721 gaagttatga tgttgaatct ctggtagaaa taaatatcga cagagagcta ttgaaaaaag
     3781 aaatatttga aatgaattca ctttatagag aggtacttgt gctattctac tttgaagagc
     3841 tttctataaa agaaatttca aatttattga atgaaaagga aggaacaatt aaaagcaagt
     3901 tatcaagagg gagaaatata ttgaaggaaa gccttttgaa aggaggcagg taatggataa
     3961 gaaagataaa ttaggaatag aaataaaaag tattatggaa gaagaaacgc ttgaccttac
     4021 tctgtctcaa aaagcctttg ataatattat tcaacacaga aagaagtctt taagagaaaa
     4081 aattcaggag tttataaaca aagagattga aatacccgta gcacccgcaa taatagggtt
     4141 tgcagccttg ttagctgtta cattgattcc gatggatata ttcaaatcac aaaatgaaag
     4201 aattatcgac atgggcgggt cttatgtaat aattagggaa ggttttgagg tgagcaagaa
     4261 atgaagataa aaattaaaat taaaacatta atttttgcag tatttatact tacgttgact
     4321 tttatatggg caataccgac agcaacctta atgattgcag gatatcttga gactaaagat
     4381 tccgagaaag cttctctttt ttacgaaaaa tatgcatcct atccgaccac gccaagtata
     4441 gaaagcaagt atcttcatgc aaacagcctg ataaggagtt ttaacaagtt tacgattttt
     4501 ctgacaggct ggggcggagg tgaaaataca agccctgaag acatagaaaa agctaaacaa
     4561 attcttatgg aaacattagc ggaaaaaccc gttaaaaaca gagaaaaaga ttattatata
     4621 aaatcatata aaatgcttct tgatattttg attgcatcag gagatgcaga aacccttcaa
     4681 gaatggatag cctttggaca aaaaggtaca gatgaaagat tagtatatac tgctgatgta
     4741 tatgatggat ttcttcatca cgtaaatggg gaaggtgaag cggcaaagtc aattattgaa
     4801 aaatatgatg agacagaatt aaaagacact atcttttatg tattaaaagc agaaattgct
     4861 ttatttgatg gaaaatatga agaatcggaa aaaatatatg ataatatata taaaaataat
     4921 tggagagaat taggaaaaag cttcggttcc ggaggatatt acagaaatga ttggtttgac
     4981 agatttatgg aagacttcaa aggagataat gtcataacag ggactgtaac ctgtgaagga
     5041 aagcccatgc cctttgttga aatatatgtg caggaagcca aagaaggatt tagtttttac
     5101 gccagcggag aaaactatgt tgctataacc gatgtaaacg gagaatttaa aacgcttggt
     5161 ttaaaagacg gattatacag tgtgggaatc ggagttgacg gctctgtcct ggcaaacaag
     5221 gcatttcaag gttcaacgat gcagtatgtt gagctgaatg gagaagatgg cgaatttcac
     5281 ctttcattca gaaataccct aaaaatatat tcacctgagc cgggagaaaa gctatcagga
     5341 gaagaattta cagtatcatg ggaagagatt gaagacgcag cctactacaa ggttgaaccg
     5401 gtggtttttt atgagccgtt cggggagaga ggaataagca cacgtatata tgcaaaagac
     5461 aagaatggag aatacaaatt tactgaaaat caagctgtat ttaacataaa tatgattaga
     5521 aacaacccat atattactgt aagtgattcg gataacggat accttggagc tcaggcggtt
     5581 cttggcatat tcattccggg agttgaatat cccattgttg taaatgcata tgatgagaat
     5641 aacaatctca taaccggcag ccttccttta aggtcttatt acgagcatat tccaagtatt
     5701 acggcagaag gaagtttgaa tgaaggagaa aattttatat atcgcaaaaa ctatcctgca
     5761 gctattgaat actacgaaaa tattctaaaa gaaaatcctg atgatataga tgcgttaaga
     5821 taccttacaa ggatatacgg aattggctgg aaggagggag agcaaaattt tgaaagagct
     5881 tttactttgg ggcagagata tactgaaatt tcaggaaaca gagaattgct gaccaatacc
     5941 cttggtggga tgaacatcag tgaaataaag gaaaacagag agaatgttta tttgactcta
     6001 aaagaagcta tgaaggaacc ggattttgat aactattatt ttttaagcag atattatatt
     6061 gctgttgaaa attatgaagg tgcaagagaa gctttgcaaa tggcagaaaa cgtaacggat
     6121 aatttgattt tcttaaacat gtattttgga gactataagg aggctgttga aagtataaaa
     6181 tcggaaagat tctacaggtc aaggttgtca tcaaataaag taaaagatgc cattgaggct
     6241 cttgatgaaa gtcctccaag gggagatgaa ttaaaggtat ttaatgactt cttgctaaaa
     6301 ctgataaacg gcattccata tggtgaaacc cggaatattt ataataaaac cgtaaagcaa
     6361 attt
//
LOCUS       DAOSOW010000060         6319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_12439_length_6319_cov_3.608876, whole genome shotgun sequence.
ACCESSION   DAOSOW010000060 DAOSOW010000000
VERSION     DAOSOW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6319
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12439_length_6319_cov_3.608876"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..535)
                     /gene="rimI"
                     /locus_tag="PLD67_02600"
     CDS             complement(89..535)
                     /gene="rimI"
                     /locus_tag="PLD67_02600"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOT21449.1"
                     /translation="MGEVIIRGMLPKDIDQVMNIERICFSLPWSRASFENELKNELAY
                     YQVAEVSGKIAAYMGMWRIIDECHITNVAVLPEYRNKGIGGRLIEKMIEICRCSEISF
                     MTLEVRVSNTPAINLYTKYGFFAVGKRPNYYVKPVEDALIMWKQID"
     gene            complement(545..1234)
                     /gene="tsaB"
                     /locus_tag="PLD67_02605"
     CDS             complement(545..1234)
                     /gene="tsaB"
                     /locus_tag="PLD67_02605"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOT21450.1"
                     /translation="MKILSIESASVTASCAVSDDEKILGEYTLRHKKTHSEKLMPLIE
                     ELMGELNLKINDIDVISISKGPGSYTGLRIGAAIAKSLAYAADIKIASIPTMKSLAAN
                     VYNEHKLIVPIMDAKAGRIYTGIYKWDNGECKEVLEQFPCNIDELIQILNEYEEPIIF
                     NGDGSVNYRKRIEDGLNKKAYFAPEVFNYLKASTLAFIGYKMAVQGDIVSANEFNPQY
                     LRLSQAERNRK"
     gene            complement(1215..1682)
                     /gene="tsaE"
                     /locus_tag="PLD67_02610"
     CDS             complement(1215..1682)
                     /gene="tsaE"
                     /locus_tag="PLD67_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOT21451.1"
                     /translation="MKIIVNNLKDTEKLGRIIAKCAEKGTVICLDGELGAGKTSLARF
                     IAKELGVKDYIVSPTFTIIKEYEGRLPFYHMDVYRIDSEDDMYDLGYDEYIYSEGVTV
                     IEWSKLIEGILPEERINIEIKRINDTKREINIDGKGFIFEKLVSELSNENTFN"
     gene            2126..3478
                     /gene="murC"
                     /locus_tag="PLD67_02615"
     CDS             2126..3478
                     /gene="murC"
                     /locus_tag="PLD67_02615"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOT21452.1"
                     /translation="MNYNFNHIYFIGIGGISMSALAEIMLEEGIKVSGSDRSDSKIIR
                     KLQTSGAKIYLNHDSKHISIDIDLVVYTSAISDDNPELIRAKELNIEIMDRAEFLGFV
                     MKNYKNAICVSGTHGKTTTTGMLSSVLIETDLAPTIFLGGEMDSLGGNLLHGSYELLL
                     TESCEYKRNFLKFNPTMEVVLNIEEDHLDYYKDLNDIESAFVEYADKLPPDGYLVVNE
                     SYRHLFDNVKCNIVTFGLDKGAYYARNIKLVPEPSYTLMHGDEEIAEIKLKVYGEHNI
                     LNSLAAAAASLTLGIEPETVVKGLFNFKGTHRRFEYKGKLKGANVFDDYAHHPSEMKA
                     TLKTARSYNDGRIITVFQPHTYTRTIKLLDGFAEALSMSDEVILVDIYAAREINTGVI
                     HSKDIINKMKNYSKDAIYAGSFREAADIVRSMAKKGDIIITMGAGNVVDIADMLIADR
                     "
     gene            complement(3637..4521)
                     /locus_tag="PLD67_02620"
     CDS             complement(3637..4521)
                     /locus_tag="PLD67_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOT21453.1"
                     /translation="MEFKEWFKEKVSIVDEYLGQIIEAKENPQKIIYQAVNYSLLSGG
                     KRLRPVLFLGAYELFRDDLKKALPFACAMEMIHTYSLIHDDLPAMDNDDYRRGKLSNH
                     KKFGEARAILAGDALLNKAFETGLDAAINLKDINAIKALKLIADSSGTEGMIGGQVID
                     IDGENKISSIEDLKYMYSLKTGAIIKSSVTAGATLAGATENEIKLLENYAEKVGIAFQ
                     IVDDILDVTSTQEKLGKLIGSDAANNKTTYLTFKSIDEARKDVEKFTDEAIKSLSLFG
                     NRAIYLIELARYLTSREN"
     gene            4881..>6319
                     /locus_tag="PLD67_02625"
     CDS             4881..>6319
                     /locus_tag="PLD67_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT21454.1"
                     /translation="MKMRKIFALFLALLMVALVFAGCTQTSQPSAPQADTQPPSEEEQ
                     QADEAKSEQILRYGASGFNGIFNPIMFDNVYDGYISNIIFEKLITHTPEGEYIPGIAE
                     WKLSEDKLTYTFTLKNGIKFSDGTDLTAEDVAFTYMTIAHPEYNGPMAYAVSNFEGYD
                     EYHEGVTDEFEGIKVIDEKTISFTFKDGRAAPANIECFDYGIMSSDYYAFENWEDFLA
                     LIDKPMGSGIMTLDSWEPKQFILLKKNTNYWDPENGAKIDSLLVSEVPEESILSALQT
                     NQIDFAQISPSAENLEAAKAMDNINIVNYIGNGYTFMCFNTTRPALEDVRVRQALMHA
                     LDRESFIKVQYGEGLAAVGMAPISPASWAFPDLSELNPYSFDMEKAAELMDEAGWLME
                     SDGYRYKDGQKLAVNWLVFTEADWPKTLYEMAADTWKQLGVDLSMELMDGNSVIARTI
                     GPEPGEKDFDIYTLAFTLTIDPDPTGFLF"
BASE COUNT         1961 a         1173 c         1114 g         2071 t
ORIGIN      
        1 atctcatgag attcttcgag ctaaagctct cagaatgaca aaaatgttat tcatataaat
       61 gacaggcttc tgcttagaat gtttgtcatc agtctatttg tttccacatt atcaaggcat
      121 cctcaacagg ttttacataa tagtttggtc ttttccctac agcaaaaaat ccatactttg
      181 tatacaaatt aattgccggt gtattagata ctctaacttc aagtgtcata aaagaaattt
      241 ctgagcatct gcatatttca atcattttct caatgagcct gcctccaatt cccttgtttc
      301 tgtattcagg taagacagca acattggtta tatgacattc atcaataatt ctccacatcc
      361 ccatataagc agcaatcttt cctgatactt cggcgacctg ataatatgcc aattcatttt
      421 ttaattcatt ttcaaaggat gccctagacc atggcaatga aaaacaaatt ctttcaatat
      481 tcattacttg gtctatatcc ttaggaagca ttcccctaat aataacttcc cccatataca
      541 cctcttactt tctgtttctt tctgcttgag ataatcgcaa atattgcgga ttaaattcat
      601 ttgccgagac gatatccccc tgaaccgcca ttttatagcc aataaaggct agtgttgatg
      661 ctttaagata attaaaaact tcaggtgcaa aatatgcttt tttgtttaaa ccatcctcaa
      721 ttctttttct gtaatttacg gaaccgtcac cgttaaaaat tataggctct tcatattcat
      781 tcagtatttg aatcaattca tcgatattgc atggaaattg ttctaatact tccttgcatt
      841 ctccattatc ccatttatat atgccggtat atatccttcc tgctttggca tccattatag
      901 gtacaataag tttgtgctca ttgtagacat tggctgctag tgatttcatg gttggaatgc
      961 ttgcaatttt aatatctgca gcatatgcca aactttttgc aattgcagca cctattctta
     1021 accctgtata ggagccggga cctttagaaa ttgaaataac atcaatatcg ttaattttaa
     1081 ggtttaattc tcccatcaat tcttcgatta agggcataag tttttccgaa tgagtttttt
     1141 tatgtcttaa cgtgtattct cccagaattt tttcatcatc agatactgca caagaagctg
     1201 ttacagatgc tgattcaatt gaaagtattt tcattgctta actccgatac cagtttctca
     1261 aaaataaatc cttttccatc aatgtttatt tctctttttg tatcattaat tcttttaatt
     1321 tcaatattta ttctttcttc gggcagtatc ccttcgatta gcttagacca ttctattacg
     1381 gtaactccct cagaatatat atattcatcg tagcccaaat catacatatc atcttccgag
     1441 tcaatgcggt acacatccat atgatagaaa ggaagccgcc cttcatattc tttaataatt
     1501 gtaaatgtag ggctgacaat atagtcttta actcctaatt cctttgcaat aaaacgtgca
     1561 agagaagttt tgcctgctcc taattctcca tccaggcaaa ttactgttcc tttttcagcg
     1621 cattttgcta ttattcttcc cagcttttct gtatctttta aattgtttac tattattttc
     1681 atatgaatcc taactttata attttactac taaatatttt tcattacaat aaaaaactta
     1741 tagaacgtca tcgctatgaa taagctacgt gtcattctga gagctagccc gaagaatctc
     1801 atgagatcct tcgctttcgc tcaggatgtc attgccctag aatcctatac cacactcggg
     1861 atgacaattt tgttctaatc ataacattgc tattatacat ttatatattt taaaaatcaa
     1921 gaaataaaga tgcccttacc ctaaatagag attgccatct atggtgatcg aagaaaattt
     1981 tattgaacaa tgtttttaca catgctataa tactatgagt atttatccat ataataatca
     2041 aaatcaacaa attttattac agctgtgtta attgcaaaat aattgaataa tattaaacaa
     2101 aataatgaaa ctcggggaga ctgcaatgaa ttataacttc aatcatatat attttatcgg
     2161 aataggcgga atcagcatga gtgccttagc tgaaataatg cttgaggaag gaatcaaggt
     2221 atcgggctct gacagaagtg attccaaaat aatcagaaag cttcaaactt caggagcaaa
     2281 aatttactta aaccatgaca gcaaacacat aagtattgat attgacttgg ttgtatatac
     2341 atctgcaatt tcggatgaca atcctgagct tatacgagct aaagaattaa atatagaaat
     2401 aatggacaga gctgaatttc tcggatttgt gatgaaaaat tacaaaaatg caatatgcgt
     2461 ttccggaact cacggcaaaa caactaccac cggaatgctt tcatcggttt tgattgaaac
     2521 tgatttagcc cccaccattt ttttgggcgg tgaaatggat tccttgggag gaaacttgct
     2581 tcacggctca tatgaactcc tgctgactga atcttgtgaa tataaaagaa actttctgaa
     2641 attcaacccc actatggagg tagtgctgaa tattgaagaa gaccatcttg attattacaa
     2701 agatttaaat gatattgaaa gtgcatttgt tgaatatgca gataaactgc ctcctgacgg
     2761 gtacttggtt gtcaatgaat catacaggca tttgttcgat aatgtaaaat gcaatatagt
     2821 aaccttcggt cttgataaag gtgcttatta tgcacgaaat ataaagcttg tcccggagcc
     2881 ttcatatact cttatgcacg gtgatgagga aatagctgaa attaaattga aagtatatgg
     2941 cgagcataat attttaaatt ccctggcagc ggctgctgca agcctgacct taggaattga
     3001 acctgaaact gtcgttaagg gtttgtttaa ttttaaagga acccacagaa gatttgaata
     3061 taagggaaaa ctcaagggag caaatgtttt tgacgattat gcacatcatc cttcagaaat
     3121 gaaagcaact ttaaaaacag cccgctccta taacgatggt agaataatca ctgttttcca
     3181 gccccatacc tacacgagaa ccataaagct tctagacgga tttgccgaag ctttgtcaat
     3241 gtccgatgag gtaattcttg tagatattta tgctgcaaga gaaattaaca cgggtgtaat
     3301 acattcaaag gatattataa acaaaatgaa gaattattca aaagatgcaa tctatgcggg
     3361 aagcttccgg gaagctgcgg atatagtaag gtcaatggca aaaaaaggtg atataattat
     3421 caccatgggt gccggaaatg tggttgatat agctgatatg ctgattgcag atagataagt
     3481 gtttcatcct gagcgtaagc gaaggatctc gggttttata agatgagatt cttcgagcta
     3541 aagctctcag aatgacacgt agcttattca tagcaatgac gagagctgtg ccctcggttg
     3601 acagggcgtc atcctgagcg taagcgaagg atctcattaa ttttctctgc ttgtaagata
     3661 tcttgctaat tcaatgagat agattgctct attgccgaat aggctcaagc ttttaattgc
     3721 ttcatccgtg aatttttcca cgtcttttct tgcttcatct atacttttaa atgtaagata
     3781 tgttgtttta ttgtttgctg catcacttcc gattaacttt cccagttttt cctgagtact
     3841 tgtaacatcc aaaatatcat ctactatttg aaatgcaatg cctacttttt cggcgtaatt
     3901 ttccaatagt tttatttcgt tttctgttgc tcccgctaat gttgcgcctg ccgttacgct
     3961 gcttttaatt atagctcccg tttttaatga atacatatat ttcaggtctt cgatggagct
     4021 tattttattt tccccatcta tgtctattac ctgccctcct atcattcctt ccgtacctga
     4081 ggagtcggca attaatttca gagcctttat tgcatttatg tcctttaaat taattgcagc
     4141 atctaaacct gtttcaaagg ctttgttaag gagtgcatca ccggcaagta ttgctcttgc
     4201 ctcaccaaac tttttatgat ttgataattt gcctcttcta taatcatcat tgtccatggc
     4261 aggcaaatca tcgtgaatca atgaatatgt atgaatcatt tccattgcgc aggcaaatgg
     4321 aagggctttt tttaaatcat ctctgaaaag ctcataggct cctagaaaaa gcacgggtct
     4381 gagtctcttc cctcccgaca gaaggctgta attaactgct tggtatatta ttttttgagg
     4441 gttttccttt gcttcaataa tttgccccag atattcatct acaatactta ctttttcttt
     4501 aaaccactct ttaaattcca tattaacatt tcctttgctc tgaattcttt tatgcaatct
     4561 tatgcttcac ccgggatgcc gcaagctgtg acctcggatg agctgtcata caggtgtaga
     4621 atcttaggtt tttttaatta tatcataaat aagtaaattg taaaacaatt ctgaaagcgc
     4681 agctttaaag atacaaaccg taaatgaacg aaacatgggt aaagcctgcc ttctccaatc
     4741 aatattaaat tgactatggt ttgtgcgtcg tatataatat atctaggtta tggtaacgta
     4801 tccataaact tgtaaatttt ttatatctgc actaggctga tataatagat tcaattcaaa
     4861 aataatctag aggagctttt atgaaaatga gaaaaatatt tgcattgttt ttagcattat
     4921 tgatggttgc ccttgttttt gcaggctgta ctcagacaag ccaaccttcg gcgccgcagg
     4981 ctgatacaca accgccgtct gaagaagagc aacaggctga tgaagcaaag agtgaacaaa
     5041 tattaaggta tggcgcatcg gggtttaacg gtatatttaa tcccattatg tttgacaatg
     5101 tatatgatgg ttatatttca aacataatat ttgaaaagtt aataacgcac actcctgaag
     5161 gtgaatatat ccccggaatt gctgaatgga aattatcgga ggataaatta acctacacat
     5221 ttacattaaa aaatggaatc aaattttctg atggcacgga tttaactgca gaagacgtgg
     5281 cttttacata tatgactatt gcccaccctg aatacaacgg accaatggca tatgcagtaa
     5341 gcaattttga aggttatgac gaatatcacg agggagtgac agatgaattt gaaggtatta
     5401 aagttattga cgaaaaaacc atatcattta cttttaaaga tggtagggct gcacctgcaa
     5461 atattgaatg cttcgactat ggtattatgt cttcagacta ttatgcattt gaaaattggg
     5521 aagatttctt agcattaatt gataaaccaa tgggctcagg cataatgaca ctggacagct
     5581 gggaaccgaa acaatttatt ttattaaaga aaaatacaaa ttattgggac cctgaaaacg
     5641 gggctaaaat tgacagtcta ttggtaagtg aagttcctga ggaaagcata ttatcggctt
     5701 tacagacaaa tcaaatagat ttcgctcaaa tttcaccgag tgccgaaaac ttagaagcag
     5761 caaaggctat ggataatatc aacatcgtaa actatattgg aaacggctat acgtttatgt
     5821 gctttaacac aacaagacct gccttagagg atgtacgagt cagacaagct cttatgcatg
     5881 cacttgacag agaatcattt ataaaggttc aatatggtga aggcttggca gcagtaggta
     5941 tggctcctat atctccggca tcatgggcat tccctgattt atcagagttg aatccatatt
     6001 cattcgatat ggagaaagct gcagagctta tggatgaagc gggatggtta atggaatcag
     6061 acggatacag atataaggat gggcagaaac ttgctgttaa ttggcttgtt ttcacagagg
     6121 ctgattggcc gaaaacatta tacgaaatgg ctgctgatac atggaaacaa ttaggtgttg
     6181 acctttctat ggaattaatg gacggcaata gcgtcatagc tcgtacaata ggtcccgagc
     6241 cgggtgaaaa agactttgat atttacacat tggcctttac attgacaata gacccggacc
     6301 ccacaggatt tttgtttga
//
LOCUS       DAOSOW010000061         6076 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_13113_length_6076_cov_3.658196, whole genome shotgun sequence.
ACCESSION   DAOSOW010000061 DAOSOW010000000
VERSION     DAOSOW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13113_length_6076_cov_3.658196"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="PLD67_02630"
     CDS             complement(<1..998)
                     /locus_tag="PLD67_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="MutS2; MutS-II; involved in blocking homologous and
                     homeologous recombination; has ATPase activity stimulated
                     by recombination intermediates; inhibits DNA strand
                     exchange; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOT21455.1"
                     /translation="MKEKSKSVLEFDKIIEKVAGFAETKMGKEEVYKLDTSHNLEGVK
                     FKQEQTAQALSIIIEKGSPPLGGISDIKEYVKRGAVGGIISLRGLLNCADTLRAARLL
                     KNYVLLNNDDRNYDLLEDLCNSIYVNKDIEERIYSVIISEEEIADDASPELKRIRREI
                     QIKNNSIKNKINSIVSSSSMQKYLQEAIVTMRNDRYVIPVKKEYRSMVKGIIHDQSAT
                     GSTLFIEPMALVEMTNDIANLKIEEKKEIERILLELSMMIGDISEEMLNNQEILKELD
                     FIFAKGKYAISINGIEPKINDRGYIRIKKGRHPLISPELVVPIDAVLGDEYTTLIIT"
     gene            complement(1213..3384)
                     /locus_tag="PLD67_02635"
     CDS             complement(1213..3384)
                     /locus_tag="PLD67_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HOT21456.1"
                     /translation="MSVELLAPAGSIESFYAAVNSGADSVYLGGKFFNARYNSRNFDN
                     DEMKHIVKYAHNKGVKVYVTMNTVLKDNEITDALNYAIFLYENDVDALIVQDLGLFCL
                     VNKYLPDFPVNISTQASVYDEYGVKFFEEFNVKKVIMARELSLTELKETVKNTDADLE
                     VFIHGALCTCYSGQCYMSSFLGGRSGNRGKCAQPCRLNYRFYDKETKEEIESFKEVPL
                     LSLKDFIAGETIYELIEAGIKTFKIEGRMKGPAYTSSVVEYYRQIIDNYYSGKNIDST
                     HLKEKAMETFSRGYTNGYLKPSKDDEMFAKLSSGIKGENIDEIEEDVKDKAQEYSRFR
                     RNKISFDIELVIGEYALLKVFDGKNTVEVKSKEVCESSIKNNVTDEIIYEQLSKLGNT
                     IYELHDLKIVKDEGVFIRKSSLNQLRRDATEILYEKCSKFYDRRKVNTLTYNDIFSNI
                     NKESHSAKISLKINSIDELKYADDVKVKRLYVPFYLNIEEVKNTKVVEKYLWVPNIVS
                     KENYKILKENLNLYDEIFDGVCVNNVGSFYFFKKNSRLKLHCGPFFNIINTFSAELLK
                     EKGAHSFTFSVETNIRDMESIVKHTSLNSEIVAHEYVQLMVMKNCPMSVIKNCKNLQD
                     CEKCSFKSKYQLKDRKGIHFNIERQNKLTHIYNSVPLSIIGIANNFVEKNIDYFLVDT
                     KFLEDAEEIIDTLYCEIKGVKTHYILEENKFTRGHYLKNVL"
     gene            complement(3420..4499)
                     /locus_tag="PLD67_02640"
     CDS             complement(3420..4499)
                     /locus_tag="PLD67_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21457.1"
                     /translation="MNKIEIVVGDKKYSVRTDESPEYVRKIESVLNDQINIIANQNKR
                     FNEIDKLILSSFVVIDRYLKLSDEINEYKKDICEEILSLKEAKELSEKEKEEAVNKAA
                     DAIIEKERFKEKLMARDNDREYLNSQITKLQEKVSEQEQQLLKSEMLINELKLKNEEL
                     IEVNEELIKERENFTKEISFMNNTKASLNGRISKLQLKLNEKEQEIIKLEKTIRDLKS
                     AVDDKSQKIYNFSDEQQKMNLLVDSKEKDINSLINKINLLQNKLNEKDESIASKDKLI
                     NDLKSTEEVLKQKYESINDEKEKYLEELLMINSDKESLINSINQLQEKLNRKEAENFQ
                     NQLEINKLKKDNMELMQLLDEETAK"
     gene            complement(4624..>6076)
                     /gene="dxs"
                     /locus_tag="PLD67_02645"
     CDS             complement(4624..>6076)
                     /gene="dxs"
                     /locus_tag="PLD67_02645"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOT21458.1"
                     /translation="GMAFEAINDAGRSNTKLLIILNDNEMSISKNVGGLSRYLSRIRT
                     TKGYLSTKRDVEKILNKIPVGKQIKSFLKRAKDGIKQIVVPGMLFEELGLTYMGPIDG
                     HNIHKIIESLERAKNIEGPLLMHVITKKGKGYKFAEERPNEFHGVSAFNIETGEALKK
                     SSQSYSDYFGMKMCEIAEKNENVVAITAAMTDGTGLRDFAKKFPNRIYDAGIAEQHAV
                     TMAAGLAVSGVVPVVALYSSFLQRAYDQIIHDVALQNLHVVFAVDRAGLVGNDGETHQ
                     GVFDEGFLSLIPNMTVLSPADYRELGNMMEFAVSYNGPVAIRYPRGSMSEYIKNSEQS
                     IVLGKGYIVEEGKYITIAAAGKMVKTAIETREILKTKNIDAEVLNLRFIKPFDKELII
                     NSVNKTKSLVIIDEATVFASYAVNIYNILPKNLDVMVKTLPDEFIKQGSIEELLKENQ
                     LDADSIAAEIIKWKADNYDIDISNNYTCLKQGR"
BASE COUNT         1626 a         1219 c          833 g         2398 t
ORIGIN      
        1 gttattatta gggttgtata ttcgtcacca agcactgcat cgataggtac caccaattca
       61 ggtgaaatga gaggatgtct tccctttttt atccttatgt atcctctgtc atttatcttg
      121 ggctcaattc cgtttatgct tatggcgtat ttgccctttg caaaaataaa gtcaagctct
      181 tttagtattt cttgattgtt tagcatttct tcacttatat cacctatcat catgctaagc
      241 tctaacagta tacgctcgat ttcctttttt tcttcgattt ttaaattggc aatatcattg
      301 gtcatttcca caagggccat aggctctata aaaagcgtgg agcctgttgc agactggtcg
      361 tgaattattc ctttgaccat acttctgtat tctttcttca cggggattac atatctgtca
      421 ttgcgcatgg ttactattgc ttcctgcaga tatttctgca tggaagatga gcttacgatt
      481 gaattaattt tattctttat tgaattgttt tttatttgaa tttctcttct tatgcgctta
      541 agctccgggc tagcatcatc ggcaatttct tcctcgctga taataaccga atatattctt
      601 tcttcaatat ccttgtttac atatatactg ttacacaagt cttccagcaa atcataattt
      661 ctgtcgtcat tattcagaag gacataattt tttagaagcc ttgcagctct tagggtatcg
      721 gcacagttca atagccctct tagagatata atacccccta cggctcctct tttcacgtat
      781 tcctttatat ctgaaatacc tcccaaggga ggtgaacctt tttcaatgat tatggaaagt
      841 gcctgtgctg tttgctcctg cttgaatttt acaccttcca ggttgtgaga tgtatctaat
      901 ttgtacacct cttcttttcc catcttggtt tcagcaaatc cggcaacctt ttcaattatt
      961 ttatcaaatt ccaaaactga tttagatttt tctttcatcg tttttccttt cctgtttgtc
     1021 attgctatga ttagcatttt gtcattctga gagctttatc tcgaagaatc tcatgagatc
     1081 cttcgctttg ctcaggatga caatcacaga gaatattcgt ttatcgtggt gtgataaaca
     1141 cgtgataatt ctgagagctt tagctcgaag aatctcatga gatccttcgc tttgctcagg
     1201 atgacaagtg tgttacaaca cattcttcaa atagtgccct ctcgtaaatt tgttttcctc
     1261 caaaatataa tgagttttaa ctccctttat ttcgcagtac aatgtatcta ttatttcttc
     1321 cgcatcctcc aaaaatttcg tatcgactaa aaaataatca atattttttt caacgaaatt
     1381 atttgcaatt cctataatcg acaggggcac gctgttatat atatgagtaa gtttattttg
     1441 tctttcgata ttaaaatgaa ttccttttct gtcttttaat tgatatttgc ttttgaagct
     1501 gcatttttca cagtcctgca ggtttttaca attctttatc accgacatgg gacaattttt
     1561 cataaccata agctgtacat attcatgggc gacaatttcc gagtttaatg atgtgtgttt
     1621 cacaatactt tccatgtccc tgatatttgt ttctacagaa aatgtaaagc tgtgagctcc
     1681 cttttccttt aaaagttctg ctgaaaaagt attgataata ttaaaaaacg gtccgcagtg
     1741 aagcttcagc ctgctatttt tcttaaagaa ataaaaactt ccaacattgt ttacacatac
     1801 accgtcaaat atttcatcat acaagttcaa attttctttt aaaattttat aattttcttt
     1861 agatactata tttggaaccc ataaatattt ttctacaact ttagtatttt ttacttcttc
     1921 aatatttaaa taaaaaggca catataagcg cttaacctta acatcgtcag catactttaa
     1981 ttcatcgatg ctgtttattt taaggcttat ttttgcagag tgactttctt tattgatatt
     2041 tgaaaatata tcattgtatg taagagtatt aacttttctt ctgtcataaa atttgctgca
     2101 tttttcgtaa agaatttccg tcgcatcacg ccttaattga ttcaaagaac tttttctgat
     2161 aaaaacacct tcatctttta caatctttaa atcatgcagt tcatatatag tattacctaa
     2221 ctttgagagc tgctcataaa ttatttcatc tgttacattg tttttaatgc tgctctcgca
     2281 gacctcttta ctttttactt caaccgtgtt tttcccgtca aaaactttta acagggcata
     2341 ttcgcctatc acaagctcta tgtcaaagct tattttattt ctcctgaacc ttgaatattc
     2401 ctgtgcctta tcctttacgt cttcttctat ttcatcgata ttttctccct ttatgccgga
     2461 acttaatttc gcaaacattt catcgtcttt tgaaggcttt aaatatccgt ttgtataacc
     2521 tctgctgaat gtttccatag ccttttcttt tagatgagta ctgtcaatat ttttgccgct
     2581 gtaataatta tctattattt gtctgtaata ttccacaact gatgaagtat atgcaggacc
     2641 tttcatgcgc ccttctattt tgaaggtttt aattcctgct tcaataagct cataaattgt
     2701 ttcccctgct ataaagtctt ttaaactaag cagaggcacc tctttaaaac tttcaatttc
     2761 ttcctttgtt tctttgtcat aaaatctgta gtttaatctg cacggctgtg cacatttccc
     2821 tcggtttccg cttcttccac ccaaaaagct tgacatgtag cattgtcccg aatagcatgt
     2881 gcaaagggca ccgtgaatga atacttccaa atcggcatct gtatttttaa ctgtctcttt
     2941 tagctctgtt aaggacagtt ctcttgccat aataactttt ttgacattaa attcttcaaa
     3001 aaatttaaca ccgtattcat cgtatacact tgcctgtgtg ctaatgttta cgggaaaatc
     3061 gggtaaatat ttatttacca ggcagaacaa acctaaatct tgaactatta aggcatctac
     3121 atcattttca tacaaaaata ttgcatagtt caatgcatcc gtaatttcat tatcctttaa
     3181 aacagtattc attgtaacat atacttttac acccttgttg tgagcatatt ttacaatgtg
     3241 cttcatttca tcattatcaa aattccggga attatatctt gcattaaaaa actttccgcc
     3301 taaatatact gaatctgctc cgctgtttac agctgcataa aatgattcaa tactgcctgc
     3361 cggcgctaat aattctacgc tcatttcttc tcctgtttaa aaaagaacgc cgacgcgttc
     3421 tattttgctg tttcttcgtc taaaagctgc ataagctcca tattatcctt tttcagctta
     3481 ttaatttcca actggttctg gaagttttcc gcttccttgc ggtttaattt ttcctgcagc
     3541 tgatttatgc tgttgataag gctttcttta tcgctgttta tcattaaaag ctcttctaaa
     3601 tatttttctt tttcatcatt aatcgattca tatttttgtt ttaatacttc ttcagtgctc
     3661 tttaaatcat ttattaattt atccttggaa gcaattgatt catctttttc attaagctta
     3721 ttttgcagca aattgatttt atttattaat gaattgatat ctttttcttt cgaatcaacc
     3781 aataagttca ttttttgctg ctcatcgctg aaattatata tcttttgtga tttatcatct
     3841 actgcgcttt ttaaatccct gatagtcttt tcaagtttga ttatttcctg ttctttctcg
     3901 ttaagtttta actgcagctt tgaaattctc ccgtttaagc ttgcttttgt attgttcata
     3961 aaacttattt cttttgtgaa attttctctt tctttgatta gctcttcatt aacttcaatc
     4021 agctcttcgt tttttaattt taattcattt atgagcattt cgctcttcaa gagctgctgt
     4081 tcctgctcac tgactttttc ctgaagcttt gttatttggg aatttaaata ttcacggtca
     4141 ttgtctcttg ccattagctt ttctttgaat ctttcttttt caataatggc atccgctgcc
     4201 ttatttacag cttcttcctt ttccttttcg gataattctt ttgcttcctt taaagataga
     4261 atttcttcac atatatcttt tttgtattcg ttaatttcat cggaaagttt taaatacctg
     4321 tcaataacca caaaagatga cagaattaat ttgtctattt cattaaaacg cttattctga
     4381 ttagctatta tattaatttg atcgttcaaa acagattcta tttttcttac atattcggga
     4441 ctttcatcag ttcttacact atatttttta tctccaacaa caatttctat tttattcata
     4501 ttcaaatccc ctttattttt tatcgtgagt aaaatatatc cgtaactata atataaatga
     4561 aaacccttaa aatttcaata gtaaataata cattttcact aatttaataa ttctacttcc
     4621 gctctaacgt ccttgtttta agcaagtata attattgctt atatcaatat cataattatc
     4681 tgccttccac ttaattattt cagcagctat cgaatcagca tctaattgat tttcttttaa
     4741 gagctcttca atcgagcctt gtttaataaa ttcatcgggc aaagttttaa ccattacatc
     4801 aagattttta ggaagtatat tgtaaatatt aactgcatag gaagcaaaaa cagttgcttc
     4861 gtcgattatt accaatgatt tcgttttatt tacggagttt attataagct ctttgtcaaa
     4921 tggttttata aatcttaaat taagaacctc tgcatctata ttttttgttt ttaaaatttc
     4981 ccttgtttct attgccgtct ttaccatttt accggctgca gctattgtta tgtacttccc
     5041 ttcctcgact atgtatccct tgccgagaac tatactttgc tcgctgtttt tgatatattc
     5101 actcatgctg cccctcggat atcttattgc tacaggtccg ttatatgaaa cggcaaattc
     5161 catcatattt cctaattccc tgtaatcagc gggtgataat actgtcatat tgggaattaa
     5221 actcaaaaaa ccttcatcaa acacaccttg atgcgtttcg ccgtcatttc ccaccaaacc
     5281 tgccctgtca acagcaaaaa caacatgaag gttttgtaag gcaacatcat ggataatttg
     5341 gtcataagct ctttgtagaa atgacgaata aagtgcaaca accgggacaa ccccgcttac
     5401 ggcaagccct gcagccattg tcaccgcatg ttgctctgca atgcctgcat cataaattct
     5461 attggggaat ttttttgcaa aatccctaag ccccgtacca tcagtcatag ctgcggttat
     5521 tgcgaccaca ttttcatttt tttccgcaat ttcacacatt ttcattccaa aataatcgga
     5581 ataagattga gaggattttt ttagagcttc tcctgtttct atattaaaag cagaaacacc
     5641 atggaattca ttcggtcttt cttcagcaaa cttatagccc ttgccttttt tcgtaattac
     5701 atgcattaat aaggggcctt caatattttt tgccctttcc agggattcaa taattttatg
     5761 tatattgtga ccgtcgatag gtcccatata ggtaagcccc aattcttcaa aaagcatccc
     5821 cggaacaact atttgcttaa ttccgtcttt tgctcttttt aaaaatgatt taatctgttt
     5881 accgaccgga attttattta atattttttc gacatccctt ttagtggaca aatatccttt
     5941 ggtagttctt attctgctta aatagcggga caagcctcct acgtttttgg atatggacat
     6001 ttcattgtcg ttaagaatta tcaataattt tgtgttgctc cttcccgcgt cgtttattgc
     6061 ttcaaaggcc attcct
//
LOCUS       DAOSOW010000062         6049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_13205_length_6049_cov_4.011512, whole genome shotgun sequence.
ACCESSION   DAOSOW010000062 DAOSOW010000000
VERSION     DAOSOW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13205_length_6049_cov_4.011512"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="PLD67_02650"
     CDS             complement(<1..594)
                     /locus_tag="PLD67_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOT21459.1"
                     /translation="MKNKLILVLCLLLIATIVLAGCASPSGDLMKGIISKNKDRVPES
                     MDIELNQAILNFTWNLFKESSENPGNIMISAPSAYFALAMTLNGAANETKNEMLKALS
                     ADNISLEDLNTGLNDWMILISKNRTAKFSITNSIWLREGFNANKDFLQTNADYYSAYI
                     RSLDFGDASAPDEINKWVKEATNDTIDKIVDEISDDLM"
     gene            complement(678..2348)
                     /locus_tag="PLD67_02655"
     CDS             complement(678..2348)
                     /locus_tag="PLD67_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT21460.1"
                     /translation="MANINNILKGIEKDIEDILFAYVKTESFTNTINEKKAEEFFLDY
                     FSKIPYFKNTPGSYGIYEIENDALNRNVSYAFLKGRGQDTIVFIHHNDIVGVEDFKLL
                     KKYAFSPKELKAQLLRIKNTLSEDAKKDLEDDTFIFGRGVCDMEGGGSIQIALLKRYS
                     ELENFNGNIVLIAVPDEENLSAGMRSAVRLLSELQDKYNLKYKLMINTEPHQRKDDET
                     GVFSEGSVGKIMPFVYVRGYLAHAGKVFEGLNPIHIMSKVVSRTELNMDFSDVVGKEA
                     APPPTWLYLKDSKEQYDVSMPLSVKGCFGILTLNQTPQSILEKVKNICEESFDEVIDD
                     MNNSFRRFAKATGLPLKYLQWESKVVNFQELFKEAEAAYGDEFIREYNKELEILGKKL
                     KGNEISIIHCNFELIEFIYGYIDDIKPRIVYGLIPPYYPNVSNLFIDDLDENVSSLCD
                     NLIKFTQEEFNQNYNTEYFYTGISDLSYSSIEKSGEIYKALEESMPLLGQFYNVPVEL
                     IEKISMPCINIGPWGKDFHKMTERVNKEDLYIRTPRIINKAISLILGN"
     gene            complement(2402..3442)
                     /locus_tag="PLD67_02660"
     CDS             complement(2402..3442)
                     /locus_tag="PLD67_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21461.1"
                     /translation="MNTENIKIAILGFGNVGRAFAKLLTEKQEEILNKFNLNIDVVAI
                     STRTKGCLVNENGIDLKEVLNDIEKLNHFDNKRSDYRDISSMEIAENVDYDILFELTP
                     LEIFSGQPAIDHIKAALSRKKHAVSANKGPIAWAYESLRDLAEKNKVLFYHETTVMDG
                     TPLFNLVDETLKFCKIKEVKGILNSTTNFVLEEIAKGKPYKEVIEEGKKRGFVEEDPA
                     MDIEGWDAAAKTAALLNVLMGANITPFDVDRKGIEDIDYEQIKEAEKRGNMIKLVCYG
                     GIEDGKVVARVKPEEVSKNSLLATIRGTTSIVSITTDLMGTISVVEHEPEIEQTAYGV
                     FSDLVRVLSNRI"
     gene            complement(3439..4584)
                     /locus_tag="PLD67_02665"
     CDS             complement(3439..4584)
                     /locus_tag="PLD67_02665"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002589596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOT21462.1"
                     /translation="MNKAVLIPDSFKGTLSSIEICTIISDKIYKHFPHCHVVSIPVAD
                     GGEGSVDCFLSALGGEKIVETVSSPYFEDMESFYGLIDDTAVIEMAACAGLPLVEDRK
                     NPSLTTTYGVGQLIMSAAKKGCKKIIVGLGGSSTNDGGCGAAAAVGVKFYDKKGNEFI
                     PIGKTLIDIDRIDLSHKAKELDNIEIITMCDIDNPMYGTTGAAYIFGPQKGADEKMIL
                     ELDKGLKNLCDVIERETGKDLKDVPGSGAAGAMGAGMIAFFNSVLQMGIETVLDTVKF
                     DEIIEDADMIFTGEGRIDSQSLRGKVVIGVGKRAKNKNIPVTVIAGGAANNIDDAYDM
                     GVTSIFTINRLPEDFEISRHKSKENLEATIDNILRLIKSVYIERGVQ"
     gene            complement(4604..5863)
                     /locus_tag="PLD67_02670"
     CDS             complement(4604..5863)
                     /locus_tag="PLD67_02670"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOT21463.1"
                     /translation="MSKFVIEGGTKLKGKVNIGGFKNAAVAIIPATLLCPERCIIENV
                     PKIQDVNVFLEILRELGAEADYINDTSIYVDASNVKNCYLENGLSKKMRASYYLLGAG
                     LGRFNGASCLMPGGCDIGERPIDQHIKGFEALGGKVTLTPDGRISVQADRLVGAKIYL
                     DVVSVGATINIMLAAVYAEGRTIIENAAKEPHIVDTANFLNSMGADIKGAGTDVIRIN
                     GVKELKGCTYKVIPDQIEAGTYMIAAAATKGDIIIDDIIPKHMEPITAKLKEMGMGIE
                     EYGDSMRVYYEKELVPVNIQTLPYPGFPTDLQQPMTVLLASLCGESTVVEGVSQDRFR
                     YVEELKKMGACIEFTKKQARIKGTKNLRGTVVQATDLRAGAALIIAGLIADGLTEVTN
                     IQHIDRGYEHIENKFMNLGAKIKRVED"
BASE COUNT         1652 a         1339 c          827 g         2231 t
ORIGIN      
        1 cattaaatca tcgcttattt cgtcaactat tttatctatg gtgtcatttg tagcttcctt
       61 cacccatttg tttatctcat ccggtgccga agcatcgcca aagtccaagc tccttatata
      121 ggctgaataa taatcagcat ttgtttgaag gaaatcttta tttgcattga aaccttctct
      181 cagccaaatt gaatttgtta ttgaaaattt agccgttcta ttctttgata tcaatatcat
      241 ccaatcatta agacctgtat ttaaatcttc caaagatata ttatctgcac tcagtgcttt
      301 aagcatttca tttttggttt catttgctgc accgtttaat gtcatagcta atgcaaagta
      361 tgcagatggt gctgatatca taatgtttcc cggattttca ctgctttctt taaacaggtt
      421 ccaggtaaaa ttcaaaatag cttgatttaa ttcaatatcc attgactcag gtaccctatc
      481 cttatttttt gatattattc ctttcatcaa atctcccgat ggagatgcgc agcctgctaa
      541 aacaatggtt gctataagca gtaagcataa tactaaaatt agtttattct tcatgtaaaa
      601 tacctccttt gtaaattaga cggatttaac cgtgataatg ttcctttgga ggtcacagag
      661 ttatatgatg gtgaatatta attgcctaat attaaggaaa ttgctttgtt aattatcctt
      721 ggagttctta tgtataaatc ttctttattg actctttcag tcatcttgtg aaaatccttg
      781 ccccatggac cgatatttat gcatggcatg gatatctttt ctattagttc aaccggaaca
      841 ttataaaatt gtcccaacaa cggcattgat tcctccagtg ccttatatat ttccccactt
      901 ttttcaatgc ttgaataact taaatcggat attcctgtat aaaaatattc cgtattataa
      961 ttctgattga attcctcttg tgtaaacttt attaaattgt cacataaaga actgacattt
     1021 tcatccaaat catcaataaa taaatttgat acattcggat aatacggagg tataagtcca
     1081 tagactattc tcggctttat atcatctata tatccgtaaa taaattcaat caactcaaaa
     1141 ttgcagtgaa ttatggatat ttcatttcct ttcaactttt tacctaatat ttctaattct
     1201 ttattatatt ctcttataaa ttcatcaccg taagctgctt ctgcttcttt gaacaattcc
     1261 tgaaaattta caacctttga ttcccactgc aagtatttta aaggaagacc ggttgctttt
     1321 gcaaatcttc taaaactgtt attcatatca tcaattactt catcaaatga ttcttcacat
     1381 atatttttta ctttttccaa aatgctttga ggtgtttggt ttaatgtcaa aataccaaaa
     1441 catcctttta cgcttaatgg cattgatacg tcgtattgct ccttgctgtc ttttaaatac
     1501 aaccatgtag gaggcggtgc cgcctcttta cctaccacat cgctgaaatc catgtttaat
     1561 tcagtcctgc ttacaacctt gctcattata tgaataggat ttaatccttc aaaaaccttt
     1621 cccgcatgag ccaagtatcc tcgaacatat acaaaaggca ttatttttcc cacggagcct
     1681 tccgaaaaaa ctcccgtttc atcatctttt ctttgatggg gctctgtatt aatcataagc
     1741 ttatatttta aattatactt atcctgcaat tcactcaata gtcttactgc cgaacgcata
     1801 cctgcagaaa gattttcctc atcaggcact gcaatcaaaa caatatttcc gttgaagttt
     1861 tccaattcgc tgtatctctt caacaatgca atttgtattg agccgccgcc ctccatatcg
     1921 catacacccc tgccgaatat gaatgtatca tcctccaaat cctttttcgc atcttcgctg
     1981 agagtattct ttattctaag caattgagcc tttaactctt tcggtgaaaa tgcatatttt
     2041 tttaagagtt taaaatcttc cacacccaca atatcattat gatgaataaa tacaatcgta
     2101 tcctgacccc tgccctttaa aaatgcatag cttacattcc tgttaagtgc atcattttct
     2161 atttcataga taccatagga gcccggcgta tttttaaaat atggaatctt tgaaaaataa
     2221 tccaggaaaa attcttctgc ctttttttca tttattgtat tggtgaaact ttcagtcttt
     2281 acatatgcaa ataggatatc ttcgatatcc ttttcaatgc cctttagaat attatttata
     2341 tttgccatat tatacacctc ttgttaagat aaagaaattg ccctcaattt taactggctt
     2401 ttcaaattct attgcttaat accctgacta aatcgctaaa cactccataa gctgtctgtt
     2461 ctatttcagg ttcgtgttcc actacggaga ttgtacccat caaatcagtt gtgatactta
     2521 ctattgatgt tgtgcctctt atagttgcca agagtgaatt tttacttact tcttccggtt
     2581 ttactcttgc aactacttta ccatcttcaa ttcctccgta gcaaacaagt ttaatcatat
     2641 ttcctctttt ttcagcttct ttaatttgct cgtagtctat atcttcaatc ccttttctgt
     2701 caacatcaaa aggagtaata tttgctccca tgagaacatt tagcaaggcg gctgtcttgg
     2761 cagcggcatc ccacccttct atatccattg cagggtcttc ctctacaaat ccccttttct
     2821 ttccttcttc tataacttcc ttgtatggct tgccttttgc aatttcttcc aaaacaaaat
     2881 ttgtagtgga atttaatatt ccttttacct cttttatttt acagaattta agtgtttcat
     2941 caactaagtt aaataagggg gtgccatcca ttacagtcgt ttcatggtaa aaaagaacct
     3001 tgtttttctc tgctaaatca cgcaatgatt catatgccca agcaataggc cccttgtttg
     3061 cgctaactgc gtgttttttt ctgctaagtg ccgcttttat atggtctata gccggctgac
     3121 ctgaaaatat ttcaagagga gttaattcaa aaagaatatc ataatctaca ttttcggcaa
     3181 tttccattga gctgatatct ctataatcac ttcttttatt atcaaaatga ttgagctttt
     3241 caatatcatt taatacttcc tttaaatcaa taccattttc atttactaag catccttttg
     3301 ttctggttga aatggcaacc acatctatat tcaagttaaa tttatttaat atttcttctt
     3361 gtttttcagt cagcagcttt gcaaatgccc ttcctacatt accgaatcca agaattgcaa
     3421 ttttaatatt ttcggtattc attgcactcc cctttctata tatacgcttt ttatgagtct
     3481 gagaatatta tctattgttg cttccaagtt ttctttggat ttatgcctgc ttatttcaaa
     3541 gtcctcaggc agtctgttga ttgtaaatat tgaagtaact cccatatcat aggcatcatc
     3601 aatattattt gctgctcctc ctgcaatgac tgttacggga atattcttat tttttgctcg
     3661 ttttcccact cctataacaa ccttgcctct taagctttga gagtcaattc tgccttcgcc
     3721 ggtaaaaatc atatcagcgt cctcgattat ttcatcaaat tttaccgtat ctaagactgt
     3781 ttcaattccc atttgaagta cagagttaaa aaacgcaatc atacccgcac ccatagctcc
     3841 cgctgctccg ctgcccggaa cgtcttttaa atctttgcct gtttctcttt cgattacatc
     3901 acaaagattt tttaaccctt tgtcaagctc caaaatcatt ttttcatccg ctcctttttg
     3961 aggaccgaat atatatgcag ctcccgtagt tccgtacatt gggttgtcta tatcacacat
     4021 tgttattatt tcaatattat ccaattcttt tgccttatgg gacaagtcaa ttctatctat
     4081 atctattagt gttttcccta ttggtataaa ttcattccct tttttatcgt aaaatttaac
     4141 accaacggct gcagctgcac cacatccacc gtcattggtg cttgaacctc ctaagccgac
     4201 aataattttt ttacatcctt tttttgctgc tgacattata agctgcccca ctccataagt
     4261 tgtcgtaagg cttggattct tcctgtcttc caccaggggt aaccctgcac atgcagccat
     4321 ttcaattacc gcagtgtcat caatcaagcc gtaaaatgac tccatatctt caaaataggg
     4381 tgagcttaca gtttcaacta ttttttcacc gcctaatgcc gacaaaaaac aatccacact
     4441 cccttcgcct ccgtcagcaa ccggaatcga taccacatgg cagtgcggaa aatgcttata
     4501 tattttatca gaaataatag tgcatatttc aatggacgac aatgttcctt taaatgaatc
     4561 aggaattaat actgctttat tcaaatgttt cctccccctg ttattaatct tctactcttt
     4621 taattttggc tcctaaattc ataaatttat tttcaatgtg ttcatatcct ctgtctatat
     4681 gctgtatatt tgtcacttca gtcaatccgt cagcaataag acctgcgata atcaatgcag
     4741 ctccggctct taaatccgta gcttgtacaa ctgttcccct taaatttttc gtacctttaa
     4801 tcctagcttg cttctttgta aattctatgc atgcgcccat ttttttcaac tcttccacgt
     4861 atctgaatct gtcctgggat accccttcaa ctactgtact ttctccgcat aaggaagcta
     4921 aaagcacagt cattggctgc tgcaaatctg ttgggaatcc gggatacgga agtgtctgga
     4981 tatttaccgg aaccaattcc ttttcataat aaacacgcat ggaatccccg tactcttcta
     5041 tgcccattcc catctcttta agctttgcgg taataggctc catatgtttg ggaattatat
     5101 catcaattat tatatctcct tttgtggcag cagcagcaat catgtatgta cctgcttcaa
     5161 tttggtcagg tataaccttg taagtgcagc cttttaattc ctttacaccg tttatacgta
     5221 ttacatccgt tcctgcgccc ttaatatctg cacccattga atttaaaaag tttgccgtat
     5281 caacaatatg aggctcttta gccgcatttt caattattgt ccttccttcg gcatatactg
     5341 ctgccaacat aatgtttatg gttgctccaa cgcttacaac gtcaagataa atttttgctc
     5401 ctactaatct gtctgcttgt acacttattc ttccatccgg agtaagagta acttttcctc
     5461 ctaatgcctc aaacccctta atgtgctggt caattggtct ttcgcctata tcacagccgc
     5521 cgggcatcaa gcacgatgct ccattaaatc tccccaatcc ggctccaagc aagtaatatg
     5581 aggctctcat tttttttgat aatccgtttt ccaaataaca attttttaca tttgatgcgt
     5641 ccacatatat tgacgtatca ttgatataat ccgcttctgc acctaattct ctcaatattt
     5701 ccaaaaatac atttacatcc tgtatttttg gtacattttc aataatgcac ctttcgggac
     5761 aaagtaacgt agccggtata attgcaacag ctgcattttt aaatccccca atatttactt
     5821 ttcctttaag ctttgtgccg ccttctatta caaattttga catactcttc ctcgcaatat
     5881 atattattca attcccggca gatgtatttg tgttccatcg ctaaaaataa ctttccacgt
     5941 tgggtcggaa tttatccttt caatattatg ccaattttca tcttcagcgc tataatatgt
     6001 catttcaatt ttaataattt ctttgttttt tatttccgga taagttaat
//
LOCUS       DAOSOW010000063         6044 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_13218_length_6044_cov_3.812156, whole genome shotgun sequence.
ACCESSION   DAOSOW010000063 DAOSOW010000000
VERSION     DAOSOW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13218_length_6044_cov_3.812156"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLD67_02675"
     CDS             <1..262
                     /locus_tag="PLD67_02675"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOT21464.1"
                     /translation="WPGWEAEHAALTETALMKYLYPELVRDIKVDSCEYEKLPYRILP
                     WNKRVQPSSGSYANPDGATMEIGRHLTEAVISGFTELIKKEF"
     gene            388..1551
                     /locus_tag="PLD67_02680"
     CDS             388..1551
                     /locus_tag="PLD67_02680"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT21465.1"
                     /translation="MKHKFIAKRYWKDKSTAMGQSDVIAKSFEDIINLSLGDPDLITH
                     DIIIDNSYADAKAGHTKYTDFRGDPELRQEIINYYKEEYDMDIKDEEVFVCASGCLGM
                     YLALEAIVDDGDEIILQAPCFTPYPQQVELARGVPVELPTYEEEDFQINVDRLECLIT
                     EKTKALVINSPSNPTGSCLTVEVMEKIAQIAEKYDLIVVSDDIYTSFSYQYPFVPFAS
                     LPGMKDRTIIINSFSKNFTMTGWRIGNIIAPDYIIKVIQQINENVVFTAPSISQRAAI
                     YALKNRHIIQPPMIEEYKKRMFYAAERINKIPKLSVIYPPKGSFYLFMNIKKTGLSSV
                     EAADLILKEAHVLMLPGDAFGACGEGYIRIACTVDVETLKEAFDRIEKIALLN"
     gene            1708..2922
                     /gene="dpaL"
                     /locus_tag="PLD67_02685"
     CDS             1708..2922
                     /gene="dpaL"
                     /locus_tag="PLD67_02685"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HOT21466.1"
                     /translation="MEIYEACGIKAVINNKSKGVRPYFLSEEEIKGVRKFHATIDGYE
                     ITPLVSLECLAEKLGVSKIYVKDESKRFGLNAFKGLGATYAIARLLCEKLGVDIDSID
                     YNYFKLPVVKEKIKDMVFVTATDGNHGRAVAWAAAQLGCNSIVYMPKGSSLRRLQHIK
                     DTGAEAFITDVNYDDTVRMAKKKADEIGGLLVQDTAWEGYEKVPNWIAQGYTTMAAEA
                     IDQMKSDGSMKPTHVFLQAGVGSFAGGILGYFTNIYKDEPPVTVIAEPENAACIYKSA
                     LADDGKPHSVSGDLNTIMAGLACGEPNTVTWPILRDFAKVYVSCPDYVAAKGMRILAA
                     PSGNDKKVISGESGAVGLGLLTLFLEKEELKPLKEKLKLNENSVIICFSTEGDTDPEQ
                     YRAIISQPEIFN"
     gene            2936..4327
                     /gene="argH"
                     /locus_tag="PLD67_02690"
     CDS             2936..4327
                     /gene="argH"
                     /locus_tag="PLD67_02690"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013403365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOT21467.1"
                     /translation="MKNLWGGRFSKSMEDYTADYNESISFDNILYKHSITGSKAHVKM
                     LAKQQIISNEICEEILRGLSIVEKKIENNEVEFNILDEDIMMIIEKELTKEVGEAGKY
                     LHTARSRNDQNALDETLFLRDAVKNSIKHLIDLLDVLINKAEEESHKIMPGFTHLQHA
                     QPITVGFYYMAHFQGLRRNVERFRELLKRLDKNPLGSCALAGTTLPIDRFATTELMGF
                     SEPTENALDTVASRDNIAEYLFNAALCMTHLSGFAEEIIVFNSQEFNFITIDDSFCTG
                     SSIMPQKKNPDIAELARGKSGRAIGNLITLLTVLKGTFLTLNKDYQEDKEALFDSIHT
                     LDRTVFIFARMLENITFNEDVLRAHLKKGFIEATDIAEFLVKHNIPFREAHEIVGKLV
                     KYCELKSKTFQDINKEELKELDFPVEIEDLSQFTVENCIKNRNSYGGTSYDDVKRQIN
                     NAKNFVAELKSLF"
     gene            4355..5098
                     /locus_tag="PLD67_02695"
     CDS             4355..5098
                     /locus_tag="PLD67_02695"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOT21468.1"
                     /translation="MEAIFRKKYIVQPSETDFRRQLKLSSLFLYLQDMATEHAYNIGV
                     GRDVLQKKYGIIWVLNRVKGEVIRYPMFKEEITIETWPEPPEKVQFNRNFLVYDKDGN
                     IIVRAFSQWVVIDFITRKLRRSSIIEEEFPKVDRERAIDCTLNKIKPRGKLNLGYKKT
                     VGYSDIDINEHLNNAKYIDYIMDCFSLENHKKYSVKAIDIDYIHEALPGEIIQLYTDT
                     EDAKDNMVYIEGINENTKLTTFKAQILRK"
     gene            5224..5994
                     /locus_tag="PLD67_02700"
     CDS             5224..5994
                     /locus_tag="PLD67_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HOT21469.1"
                     /translation="MISRKSVITFGMVAIPIAMYTATQDNDIHFNQLHAEDNSRIRYK
                     KTCAHCGKEITSNEIVKGYQYDKDKYVVITDEEIEKIKTEKDKSIQILHFAELNQISP
                     VYYEKTYQAVPEPGGEKAFELLRSALMAEQKIAIGKTVMGTKDTLMAIIPREEGILIS
                     TMLFADEVKEIQKQYTKPEVSKQELDMAKLLINSMDTPFDPSKYKDEYQVKLRELIEA
                     KISGREVVAAEPESAGKVIDLMEALKASVEKAKNKETA"
BASE COUNT         2158 a          913 c         1316 g         1657 t
ORIGIN      
        1 atggccgggc tgggaagcgg aacatgctgc tttaaccgag acagcactga tgaaatattt
       61 ataccctgag ttggttaggg atataaaagt agattcgtgt gagtacgaga aattgcctta
      121 tcgcatttta ccttggaata agcgtgttca accttcatcc ggctcatatg caaatcctga
      181 cggtgctact atggaaattg ggagacatct gactgaagca gttatttcag gttttacaga
      241 actaattaaa aaagagtttt aatgagttac ctataataag aatgtttata tatgctgaat
      301 actatgttaa aatatgggta aaagtgtaga tgaataacaa atctgccaat cagttaaaat
      361 aagaggcata gtaaatggag gatagtaatg aaacataagt ttattgcaaa gaggtactgg
      421 aaagataaaa gtacagccat gggtcagtct gatgtgatag cgaagtcttt tgaagatatt
      481 ataaatttaa gtctgggtga cccggattta attacacacg atattataat agataattct
      541 tatgctgatg ctaaggcagg acatacaaaa tataccgact ttcgcgggga ccctgaattg
      601 cggcaagaaa taattaatta ttacaaagaa gaatatgata tggatattaa agatgaggaa
      661 gtctttgttt gtgcatcggg atgtcttgga atgtacttag ccttagaagc tattgttgat
      721 gatggagatg aaataatact gcaggcacca tgctttacgc catatcccca gcaggtggaa
      781 ttagcaaggg gagtacccgt tgaactgcct acttatgaag aagaagactt tcaaataaat
      841 gtagatagac ttgaatgctt aattacagaa aagacaaagg cattggttat taactcacca
      901 agcaatccga ccggctcttg tcttactgtt gaagttatgg aaaaaattgc acaaatcgct
      961 gaaaaatacg acttgattgt agtttcagat gatatttata catctttcag ttatcaatat
     1021 ccatttgtgc cgtttgcaag tcttccgggc atgaaagaca gaacaattat aattaactct
     1081 ttttcaaaaa attttactat gaccggttgg agaataggaa atattattgc accggactat
     1141 ataataaaag ttatccagca gattaacgaa aatgtagttt tcacagcacc gtcgatttcg
     1201 caaagggctg caatctatgc tttaaaaaat cgccatataa ttcaaccgcc tatgattgaa
     1261 gaatataaga agagaatgtt ttatgcagct gagcgaataa ataagattcc aaaactttct
     1321 gttatttatc cgccgaaggg aagcttctac ttgtttatga atattaaaaa aacaggactg
     1381 tcaagcgttg aagcggcaga tttaattttg aaagaagctc atgtattgat gctgccgggg
     1441 gatgcctttg gagcttgcgg agaaggttat attcgcattg cctgcacagt tgatgttgaa
     1501 acactgaaag aagcattcga tagaattgaa aagatagcat tattaaatta atataaagta
     1561 tttataggga atataaaaac ttcccataat aatatttggg ataaccaaaa ggttatccaa
     1621 tattttttcg gcaggaaaat attgacctta atatacactt attatatagt aatgttagcg
     1681 ttctactact aattatgggg gtatgtaatg gaaatatatg aagcatgcgg aatcaaagca
     1741 gtaataaata ataaaagtaa aggtgtgagg ccttactttt taagtgaaga agaaataaaa
     1801 ggagtaagga agttccatgc tacaatcgac ggctatgaaa ttacgccttt ggtaagcttg
     1861 gaatgtttag ctgaaaagtt aggtgtttcc aagatatatg taaaggatga atcaaaaaga
     1921 tttggactta atgcatttaa aggcttgggt gcaacatatg ccatagcaag actgttgtgc
     1981 gagaaattag gtgtggatat agattcaata gattataact actttaagct gcctgtggtg
     2041 aaagaaaaaa tcaaggacat ggtctttgtc accgcaacag acggcaatca tggaagagcg
     2101 gtagcttggg cggcagctca attgggatgc aactcaattg tatatatgcc aaaggggtct
     2161 tccctgagga gattgcagca tattaaggat actggtgcgg aagcttttat tacggatgta
     2221 aattatgacg ataccgtaag gatggctaag aagaaagctg atgaaatagg tggattatta
     2281 gttcaggata cagcttggga aggatatgaa aaagttccca actggattgc tcagggctat
     2341 actacaatgg cagccgaagc catagaccag atgaagtcgg atggaagcat gaagccgacg
     2401 catgtctttt tacaggcggg agtgggctcc tttgcgggag gtatattggg atactttaca
     2461 aatatttata aggatgagcc gcccgtaact gtaattgcgg agccggaaaa tgccgcatgt
     2521 atttataagt ctgccttggc ggacgacgga aaacctcatt ccgtttcggg agaccttaat
     2581 accataatgg cgggattagc ttgcggagag cctaatacgg ttacctggcc tattttaaga
     2641 gattttgcaa aagtctacgt gtcttgtcct gattatgtgg cagctaaagg aatgagaata
     2701 ttggcagctc cttcaggtaa tgataagaaa gtcatatcag gagaatcagg tgcagtgggg
     2761 ttaggactgt taaccctgtt tcttgaaaaa gaagagttga aaccattaaa agaaaagtta
     2821 aaattaaatg aaaactctgt aataatatgt ttcagtacgg aaggggatac tgacccggaa
     2881 cagtaccgag cgattatttc acagccggag atttttaact gaaaggatga aaagtatgaa
     2941 aaatttatgg ggcggtcgtt tttccaagag tatggaagac tataccgctg attacaatga
     3001 atctataagc tttgataata ttttgtataa acacagtatt acgggcagca aagctcatgt
     3061 aaaaatgctt gcaaaacagc aaatcataag caatgaaata tgcgaagaaa tattgagggg
     3121 attatcaatt gtagaaaaga aaattgaaaa taatgaagtt gaatttaata tactcgatga
     3181 agatataatg atgattattg aaaaagagct caccaaggaa gtcggagaag caggaaaata
     3241 tcttcacaca gcgagaagca gaaacgacca gaatgcattg gatgaaacgc tgtttttgcg
     3301 ggatgcagta aagaattcaa tcaaacattt aatagattta cttgatgtcc tgataaataa
     3361 ggcagaagaa gaatcacata aaataatgcc cggttttact catttacagc acgctcagcc
     3421 gataacggtc ggattctact atatggcaca ttttcaaggc ttgcgaagaa atgttgagag
     3481 attccgagaa ttattaaaac gtctggacaa aaatccctta ggatcgtgtg cattggcggg
     3541 aaccacattg cccatagacc gttttgcgac aacagaatta atgggttttt cggaacccac
     3601 ggaaaatgca ttggatacag ttgcttcaag agataacatt gcagaatatt tatttaatgc
     3661 tgctctatgt atgactcatt taagcggttt tgcagaagaa attattgttt ttaattctca
     3721 agaatttaat ttcatcacta ttgacgacag cttttgtacg ggtagcagta ttatgcctca
     3781 gaagaaaaac ccggatattg ccgaattggc tcgaggcaaa tcaggtagag ccatagggaa
     3841 tctcataaca cttttgaccg ttttaaaagg taccttttta accttaaaca aggattatca
     3901 agaagataag gaagcactct ttgacagtat acatacattg gacagaacag tgtttatatt
     3961 tgccaggatg cttgaaaata taacttttaa tgaggatgta ttaagagcgc atttaaaaaa
     4021 aggatttata gaagcaacgg atatagcgga attcttggta aaacataata ttccatttag
     4081 ggaagctcac gaaatcgtgg gtaagttagt taaatattgc gagctcaaat caaagacttt
     4141 tcaggatata aacaaagaag aattgaagga gttagacttt cccgttgaaa ttgaagactt
     4201 gagtcaattt accgtagaaa actgtataaa gaacagaaac agctatggag gcacatccta
     4261 cgatgatgtt aaaagacaaa tcaataatgc caaaaatttc gtcgctgagt taaaaagtct
     4321 gttctaatca tcctgagtat gatagaggtg taagatggaa gcaattttta gaaagaaata
     4381 catagtacag ccgagtgaaa cagattttag aaggcagctt aagttaagct cgttattttt
     4441 atacctgcag gacatggcaa ccgagcatgc ctataatatc ggtgtgggaa gagatgtatt
     4501 acaaaaaaag tacggcatca tatgggtatt aaatcgtgta aagggtgaag taataagata
     4561 tcctatgttc aaggaagaaa taacaattga aacttggccg gagccgccgg aaaaagttca
     4621 gtttaacagg aattttttag tttatgacaa ggatggaaat attatagtca gggcttttag
     4681 ccaatgggtt gtaatagatt tcattactag gaaactgaga agaagcagta taatagaaga
     4741 agaatttcca aaggttgaca gagaaagagc tatcgactgt acactgaaca aaataaaacc
     4801 aagaggcaaa ctaaatttgg gttataaaaa aacagtcggt tacagtgaca ttgatataaa
     4861 cgagcatttg aacaatgcaa aatacatcga ttatataatg gactgtttca gcttggaaaa
     4921 tcacaaaaaa tactctgtca aagcaatcga tatagattat atacatgaag cattgccggg
     4981 tgaaatcata cagctttata ccgatacaga agatgcaaaa gataatatgg tatacataga
     5041 aggaattaac gaaaatacga aacttacaac cttcaaggca caaatattaa gaaaataatt
     5101 acaaaagaca cgggacgttt gttttgcttc cttgtctttt ttatatacga ttgatagcca
     5161 atacttgtca gtatgtcgaa tacataaaat ataattggca acaataacct tggaggtgat
     5221 tatgtgatat cacgtaaatc agttataact tttggcatgg ttgctattcc aattgcaatg
     5281 tatacggcaa cgcaagacaa tgatattcat tttaatcagc tgcatgcgga agacaacagc
     5341 cgaataagat acaagaaaac atgtgcacat tgcggcaaag aaataacttc taatgaaatt
     5401 gtaaaaggat accaatatga taaggacaag tatgttgtaa tcacagatga ggaaattgaa
     5461 aaaataaaga cggagaaaga taaatcaatt caaattcttc attttgcaga attgaaccaa
     5521 atttcacccg tttattatga aaagacctat caggcagtgc cggagccggg cggggagaag
     5581 gcttttgaac tgttgcgctc tgcactgatg gccgagcaaa aaatagccat aggaaagact
     5641 gtcatgggca ccaaggacac attgatggct attattcctc gagaggaagg tatattgatt
     5701 tctaccatgc tttttgccga tgaagtcaaa gaaattcaaa aacaatatac caagcctgaa
     5761 gtatccaagc aggagctcga tatggcaaag cttcttataa attcaatgga tactcctttt
     5821 gacccttcca aatacaagga tgaataccaa gtgaagcttc gtgagcttat tgaagcaaaa
     5881 atttcaggaa gagaggttgt tgctgccgag cctgaaagtg caggcaaggt aatcgacctg
     5941 atggaggctt tgaaagccag tgtagagaaa gctaagaata aggaaacggc ataatatggc
     6001 tgtaggatta aatgaatata agcaaaaaag aaattttgaa aaaa
//
LOCUS       DAOSOW010000064         6023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_13296_length_6023_cov_2.808814, whole genome shotgun sequence.
ACCESSION   DAOSOW010000064 DAOSOW010000000
VERSION     DAOSOW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13296_length_6023_cov_2.808814"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..783
                     /locus_tag="PLD67_02705"
     CDS             61..783
                     /locus_tag="PLD67_02705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT21470.1"
                     /translation="MENSRSDTIMVVSYATKNKTGDVFSIPRDTYVDRDGFINNGNNK
                     INSVYSAKGIEGLYEIILNITGIKIDKYVTIDYDGVRAAVDAIGGVEVDVPFHMRYTD
                     PYSDPPLDINIPPGKQLIEGDRAMEFLRFRKTNYAGYSGYTNGDIGRVETQQGFVKAA
                     IKKALTFRLPLVVKEVYPYVNTDFSLMEATSLAMGSIGLSAENINFHTLPGTAQTRNG
                     LSFYMLDYLATKDLIYSVLGMP"
     gene            complement(869..1084)
                     /locus_tag="PLD67_02710"
     CDS             complement(869..1084)
                     /locus_tag="PLD67_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HOT21471.1"
                     /translation="MSKNKLLVPEAQQAINDLKTEIAQELGMPVINGTTSNMYPAIVN
                     GLSVREMVRRGEQMLINKKAPSDTDIY"
     gene            complement(1307..1816)
                     /locus_tag="PLD67_02715"
     CDS             complement(1307..1816)
                     /locus_tag="PLD67_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21472.1"
                     /translation="MMSSCRNRCRRRDRDDVAGIERIALRGPGCIDGTGRCRGVLGIG
                     DRRRCEDLLDAAEDFCDEVFGDRGRRCCSNVLGTGGERFRCCNRDLLGIRDREFRCDN
                     RDLLGIRDRDFDCDNRRVMGAGDRERCGQVFGTGGGFQCNNVAGIGIRRRICRPTCSD
                     LETPTTVPR"
     gene            complement(1883..2641)
                     /locus_tag="PLD67_02720"
     CDS             complement(1883..2641)
                     /locus_tag="PLD67_02720"
                     /inference="COORDINATES: protein motif:HMM:NF026048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOT21473.1"
                     /translation="MKVIINNVEYEGLPGETLMELAKRNKVYIPNLCHKEGFEGQGRC
                     RLCMVEAKEGNRTKIVSACVYPVQDGLEVITDSDRIRAMRKNIILLLLLKTPNNEYIK
                     QLAEEYEIKAPAGYVDLSSNENCILCGLCVKACEKLGTSAISLVNRGTGKKVSTPFDD
                     PSKDCIGCGACAEVCPTNAITMTEINGIRTIWNKEFKMVKCSICKKEYTTEEALKFIE
                     THLGDEEEKICDTCRKKIISERFKESYKNIYGLP"
     gene            complement(2622..4436)
                     /locus_tag="PLD67_02725"
     CDS             complement(2622..4436)
                     /locus_tag="PLD67_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT21474.1"
                     /translation="MTSRKILVCCGTGCLANNSFEIYTSFLEKLKDTNTEVSPEIKAT
                     GCNGLCEKGPVVKILPDEISYFKVKSKDVDEIISKTISKGEIIDRLLYFDTNSKKKIV
                     SHKDANFYKHQQKIALRNIGEIDPKNIEDYKERDGFKALKKVLFEMTPDEVITEVEKS
                     GLRGRGGAGFPTGSKWRTCSKYDNYPKYVVCNGDEGDPGAFMDRSIMEGDPNTVIEGM
                     IICGYAINSNRGFLYIRDEYQLARKNMQIAIDNAGKHGFLGKNILDSGFDFDLEIVRG
                     GGAFVCGESSALMASIEGNVGEPRAKYIRSVEKGLWGMPTVLNNVETWANIPPIILKG
                     AQWFNQIGIEKNSGTKVFSLVGKVKNTGLVEIPMGTTLRELVFDIGGGIIKDRPFKAA
                     QTGGPSGGCIPAEYLDISLDFDSLSKIGSMMGSGGVIVMDDTTCMVELARYYMEFLSE
                     ESCGKCTTCREGINTMLNILNKITLGQGREEDIDLLISLSDTIKEASLCGLGKTAPNP
                     VMTTLKYFRDEYMEHIVDNKCPAGVCKNLTSYSIDRDTCIGCSACARKCPVNAIEGEL
                     KKPHIIDSDKCIKCGTCFTVCPSGAVKIGGGLIESYNK"
     gene            complement(4429..4905)
                     /gene="nuoE"
                     /locus_tag="PLD67_02730"
     CDS             complement(4429..4905)
                     /gene="nuoE"
                     /locus_tag="PLD67_02730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOT21475.1"
                     /translation="MTIETIDINTIIHNYRPEKRFTLAILQDIQNKFNFIPKEAIFII
                     SDYLKVPASEIYSMATFYKALSLKPKGRHIIKVCNGTACHIRGSNTLIGEITDLLNIK
                     PGETTADGLFSIEIVNCLGACALAPVMVVDDKYFGAMTRDKVIEVINEYKEAVEND"
     gene            complement(4967..>6023)
                     /locus_tag="PLD67_02735"
     CDS             complement(4967..>6023)
                     /locus_tag="PLD67_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOT21476.1"
                     /translation="PTRNWQESYFETADKISGETLKENHIVKQGFCHRCPIGCGRVID
                     FNGKIIGGPEYEPIWAFGANCGVDDLPAINEANYMCNEYGLDAISTPCTIAAAMELYQ
                     KGYISDEDCDGVPLKWGSGKAIVEWTKRMGQGYNELAKLMAEGSYRLCEHYGVPEYSM
                     SVKKQEMPAYDARGIQGIGITYATSNRGGCHVRGYVTSTEVLGIPMKTDPLSTEGKAA
                     LVKLFQDLTALVDSSDICLFTTFAIGLPEVSAMLRGTTGFEHTDEEMLRAGERIWNLE
                     KLFNLEAGLTREDDMLPPRLLNEPVTSGPSKGKVTELITMLDEYYQVRGWDEYGVPTE
                     EKLNELSISSRGLAEAF"
BASE COUNT         1623 a         1337 c         1076 g         1987 t
ORIGIN      
        1 gatatattta aatttataag caatgactaa gatgacagaa taaatgttct tttgcttgga
       61 ttggagaata gcaggtctga tactattatg gttgtaagct atgccactaa aaataagacc
      121 ggcgatgttt tttccatacc aagagataca tatgtggata gagacggatt tataaacaac
      181 ggcaacaata aaataaattc ggtatattca gcgaagggta tcgaaggatt atatgaaata
      241 attttgaata taacggggat taaaatcgac aaatatgtta caattgatta tgacggcgtg
      301 agagctgcgg tggatgctat tggcggagta gaagtggacg tgccctttca tatgagatat
      361 accgacccgt attcagaccc gcctctcgat attaatatac ctccggggaa acagcttata
      421 gaaggcgaca gagccatgga gtttttaagg ttcaggaaaa ctaattatgc aggttattca
      481 ggttatacta acggagatat aggtcgtgtc gaaactcagc agggatttgt aaaagcagca
      541 attaaaaaag cattgacttt tagactgccc ttagtggtaa aagaagtata cccatatgtt
      601 aataccgact tttccttgat ggaagcaaca tccctggcaa tgggaagcat aggattatcg
      661 gctgaaaata ttaattttca cacccttccc ggaacagctc aaacccgaaa cggtctgtca
      721 ttttatatgt tagattattt agcaacaaaa gatttgattt attcggtatt aggtatgcct
      781 taatatcaaa tgaacatcaa atggtgccag gtaccacatt aatgtggtgc ctggcacctt
      841 cttgtgtggc atggttttgt gctactggtt aataaatatc cgtgtcagat ggagcctttt
      901 tatttatcag catttgctcg cctctacgca ccatttctct gacgctgaga ccattaacaa
      961 tagcaggata catatttgat gtggtaccgt taataactgg cattcccagt tcttgggcta
     1021 tttccgtttt taaatcattt atggcctgtt gtgcttcagg aactaataat ttatttttgc
     1081 tcatactatc gctccttcct ttaaatgaag ctaaaaatac aggcagaaat tgccatgaac
     1141 gaagcaaaaa tattaatatc caaagcattt gaagaatcgg taccataaat taaatagcag
     1201 gatttttttg cttcgttata gttagtatta tcattgaata taaaaataaa ctgtaaaaaa
     1261 tttggaaaaa aaatagggga caccttttaa gaatgtcccc tgttgtttat cttggtacgg
     1321 ttgttggagt ttccaaatca gaacaggtgg gtctgcatat acgtcttctt atgccgatgc
     1381 cagcaacatt attacattgg aatcctccgc ctgtgccgaa tacttggccg cagcgctctc
     1441 tatcgcctgc ccccattact cgtctgttat cgcaatcaaa atctctgtct cgtattccca
     1501 aaaggtctct attgtcgcag cggaattctc tgtcgcgtat tcctaaaagg tctctgttgc
     1561 agcagcggaa tctttctccg cctgtaccta atacattgct gcagcacctt ctgcctctgt
     1621 caccaaaaac ctcatcgcag aaatcttctg ctgcatccaa taaatcttca cacctgcgtc
     1681 tgtcgccaat gcctaataca cctctgcagc gtcccgttcc gtcaatacac ccgggacctc
     1741 tcaaagcaat tctctcgatt cccgcaacat cgtctctgtc tcttcttcta catctatttc
     1801 tacaagaact catcataaca ccttattcgt tttatatttt gagccaagct cattattact
     1861 atattcaaaa ttgtacaaag tgttacggca gtccataaat atttttatat gattctttaa
     1921 atctctccga aataattttt ttcctgcatg tgtcacagat tttttcttct tcatctccta
     1981 aatgggtttc aatgaattta agagcttctt cggttgtata ttctttcttg catatggagc
     2041 atttaaccat tttgaattct ttgttccata ttgttctgat accattgatt tctgtcatgg
     2101 ttattgcatt tgtaggacat acctcggcac atgcaccgca accgatacaa tcctttgaag
     2161 ggtcatcaaa gggagtggaa acttttttgc ctgtacctct gtttaccagg gatatggctg
     2221 aagtacccag tttttcacag gctttgacgc aaagaccgca aaggatacag ttttcattgc
     2281 tgcttaggtc tacatatcct gccggagctt ttatctcata ttcttcagct agttgcttaa
     2341 tatattcatt gttgggagtc ttaagcagga ggagaaggat aatgtttttt ctcatggctc
     2401 ttattctatc agagtcggtg ataacctcca ggccatcttg tacgggataa acacatgctg
     2461 aaacaatctt tgttctgttt ccttcctttg cttcaaccat gcaaagcctg cacctgccct
     2521 gaccttcaaa tccttcctta tgacaaagat tgggaatata gactttgttt cttttggcca
     2581 gttccatcag ggtctctccg gggaggcctt catattcaac attatttatt ataactttca
     2641 attaatccac ctcctatctt aactgctcct gaaggacata cggtgaaaca ggtaccgcac
     2701 ttaatacact tgtcactgtc tataatatga ggctttttta attctccttc aatagcattt
     2761 acaggacatt ttctggcaca agcgctgcaa ccgatgcatg tatcccggtc tattgaatag
     2821 gaggtaagat ttttgcatac tcctgcagga catttattgt caacaatgtg ttccatatat
     2881 tcatctctga aatactttaa agtggtcata acaggattag gcgcagtctt gcctaaaccg
     2941 cagagggaag cttctttaat tgtgtcgctt aaacttataa gcaaatcgat atcttcctcc
     3001 cggccttgac ccagggtaat cttgttaagg atattgagca tggtattaat tccttccctg
     3061 caggtggtac atttgccgca ggattcttca gataaaaatt ccatataata cctggcaagt
     3121 tcaaccatac aggtggtatc atccattaca ataactcctc ctgagcccat catggaacct
     3181 attttgctta aactgtcaaa atcaagggat atatccaggt attccgcagg gatacaccct
     3241 cctgaaggac ctcccgtctg tgccgcttta aagggtctgt ctttaataat tcctccgcct
     3301 atatcaaata ccagttccct taatgtagtc cccatgggaa tttcaaccaa accggtattt
     3361 ttaactttac ctacaagaga aaaaactttt gttccagaat ttttttctat acctatttga
     3421 ttaaaccatt gggctccctt taaaataatc ggcggtatgt ttgcccaggt ttcaacgttg
     3481 tttaaaaccg taggcatgcc ccaaagtcct ttttccacgc ttcttatata tttagccctc
     3541 ggttctccca cgtttccttc aatggatgcc atcagggcgc ttgattctcc gcatacaaat
     3601 gcacccccgc cccttactat ctccaaatca aaatcaaagc cgctgtctag aatatttttc
     3661 cccagaaagc cgtgttttcc ggcattgtcg attgctatct gcatattttt tctggctaac
     3721 tgatattcgt ccctgatata aagaaatccc cgatttgaat tgattgcata accgcaaata
     3781 atcattcctt caataaccgt attggggtct ccttccatga tacttctgtc catgaaagcg
     3841 ccggggtctc cttcgtctcc gttacatacc acatatttgg gataattatc gtatttagag
     3901 caggttctcc atttgcttcc cgtagggaaa cctgcaccac ccctgcctct taagcccgat
     3961 ttttccactt cggttattac ttcatcagga gtcatttcaa acaaaacctt ctttaaagcc
     4021 ttaaaaccgt ctctttcctt gtaatcttca atatttttag ggtcaatctc accaatgttt
     4081 ctaagggcaa ttttttgctg gtgtttgtaa aagttagcat ccttatgaga tacaattttt
     4141 ttcttggaat tggtatcaaa ataaagaagc ctgtctatta tttcaccctt tgaaattgtt
     4201 tttgaaataa tttcatcaac atcttttgac ttgactttaa aataagagat ttcatcaggc
     4261 agaattttta ccacgggtcc cttctcgcaa agaccgttgc atccggtggc ttttatttcc
     4321 gggcttactt ctgtatttgt atcttttaat ttttccagga agctggtata tatttcaaaa
     4381 ctgttgtttg caaggcagcc tgtaccgcaa cataccaata tttttctact agtcattttc
     4441 aactgcctcc ttgtattcat taatgacttc aataacttta tccctggtca tggccccgaa
     4501 gtatttgtcg tcaacaacca tcacgggtgc aagggcgcag gctcccaggc agttgactat
     4561 ttcaattgaa aataaaccgt cggctgttgt ttcccccggt tttatattta acaaatcggt
     4621 tatttcacct ataagggtat tggaacctct gatgtggcag gctgtgccgt tgcatacctt
     4681 gataatgtgc ctgccctttg gcttcagact cagggcctta taaaaggtag ccatggaata
     4741 tatttcactg gcgggaacct ttaaataatc tgaaataatg aagattgctt cttttggaat
     4801 aaaattaaac ttgttttgaa tatcctggag aatggccaaa gtgaatcttt tttccggcct
     4861 gtaattatgg attatggtgt ttatatcaat tgtttctatt gtcatgaaat caccttcgtt
     4921 ttttgttatg aaacccggag tatgaagata ctccgggcta ttagaattaa aatgcttctg
     4981 ccaaacctct gctgcttatt gagagttcat ttaatttttc ttctgtggga actccatatt
     5041 cgtcccagcc tctaacctgg tagtattcat caagcatggt tataagttct gttaccttac
     5101 cctttgatgg gccgcttgtt acaggttcat ttaacagcct tggaggaagc atgtcatctt
     5161 ctctagttaa gcctgcttcc aggttaaaga gcttttcaag attccatatt ctttctccgg
     5221 ctcggagcat ttcttcatcc gtatgttcaa atcccgtggt gccccgaagc attgccgaca
     5281 cctccggaag tcctattgca aaggttgtga ataaacaaat atccgacgaa tcaaccaatg
     5341 ccgttaaatc ctggaataat ttaaccaggg cagccttacc ttcggttgac aatgggtctg
     5401 ttttcattgg aatacccaat acttctgttg aggtgacata tcctcttaca tggcaaccgc
     5461 ccctgttgct ggttgcatat gtgataccta ttccttgaat tcctcttgca tcataagcag
     5521 gcatttcttg ctttttaaca ctcattgaat attccggtac accataatgc tcgcaaagac
     5581 gatatgagcc ttctgccatc aattttgcca attcattata gccttgaccc attcgtttcg
     5641 tccattccac tattgctttg ccgcttcccc attttaaagg tactccatcg caatcttcat
     5701 cggaaatata tcctttttga tataactcca ttgcagcggc tatggtgcaa ggagtagaaa
     5761 tagcatccaa cccatattca ttacacatat aattggcttc atttattgca ggcaaatcat
     5821 ctacaccgca atttgctcca aaagcccaaa taggttcata ttcaggtccg ccgataattt
     5881 ttccgttaaa atcgattacc cggccgcagc ctattggaca cctatgacaa aagccttgtt
     5941 tcactatatg attttccttc aatgtttctc cggaaatttt atcagccgtt tcaaaatacg
     6001 attcttgcca gttcctggta gga
//
LOCUS       DAOSOW010000065         5896 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_13673_length_5896_cov_3.162643, whole genome shotgun sequence.
ACCESSION   DAOSOW010000065 DAOSOW010000000
VERSION     DAOSOW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13673_length_5896_cov_3.162643"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="PLD67_02740"
     CDS             <1..865
                     /locus_tag="PLD67_02740"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOT21477.1"
                     /translation="VAMTYLFVFLMLSIVVKTITPNIINSISELSQDIPRLYKKANSM
                     LSILSDELIVKIYLAEIAQKLSSILTAIINNALTKVINIFMAFANTLLSIIISVYILI
                     DKESIINWFKEFASLFIGRKAANDIVRISHILYRNVSSYISGKATASLIIAFLIYIGS
                     KYIIKCPYPAIDGMVIGITNMIPYFGTFIGSVPIVLINVLYTPQKGALLFILILILQQ
                     LESLIIDPKILGSQLSIKPLLVIISIIIGGGLFGPLGLFFAVPVAALIKSIVDVYMLK
                     SLKNVHIIDKS"
     gene            1095..3419
                     /locus_tag="PLD67_02745"
     CDS             1095..3419
                     /locus_tag="PLD67_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT21478.1"
                     /translation="MKKTLLVIIILIFVASFINIYAAVFPDIKKDPYLEEAIGVLSSY
                     KVVQGYPDNSFKPDRTVTRAEMAKVVTVAAGFNEYSKNMTSSYEDMHGHWAESYVELA
                     DVLGIVKGISPSVYGPDNLIKFEEAYTMILRLLGYTDESLMGSWPSNYREKAIELNLF
                     ENINSNTEFASRRDITIMLFNALSKNLVSVKENNTYNVTNKTLISKLGKMETRVVSIK
                     DLNIDNFDYTDYLFNKWDIYYDNYGNTVYVSNPQYNEFSGTVTSILSNRVIFVTDDYG
                     NVRVFQVPDIPIIINGERGSFNNLNESSVKIIYEEDSFKGEVIGIIAYKATDVIVVDR
                     DDLYKEGSRQFAGKNLPLSNSEVNFNKIDIKGDAKSLEDIKVNDVVYFYETQNDKNSS
                     LTLNVLRSKVEGVVTSVDNVNGATFYTINNISYMTGKDYIFTEKANVNDSVTLILDKN
                     NNIIKLYINKYCKRPSTYGIVINSSSGTNGNAEARILDEYGRIRTYSLADNSSVVRVH
                     ENDSSVLKQSLIGSNDLVMFDPVSNGTLKIINLMQTRNIASPYNNQTQTLENGGYKIS
                     SDTFIVYRNNGQYQLLVPSQLGEYLEGRAVVNYMGHIDAIYLTKGIKESNVITITPDT
                     PQNYNGTIYGIIKGVTKLDDTTSHIQFFNDSNVFSVSNTSVAGKSISSMMNSYVRAVI
                     VNGMVSSIEKVTPETSRIKISQIYTNQMLIDNITYMEYAQDVKVYVCTLDSSGNISDF
                     KEGTKSDIKAGSTAQLYNLYGGFDGVIDVVFVFK"
     gene            3624..4754
                     /gene="alr"
                     /locus_tag="PLD67_02750"
     CDS             3624..4754
                     /gene="alr"
                     /locus_tag="PLD67_02750"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT21479.1"
                     /translation="MNEKLRPAWVEINREKAIHNFLEVRRAVGPDRKICAVVKADSYG
                     MGALELSKMYLENGVDMFAVAVIGEAFELRKEIKDKDILVLGFTSEEFYDDAIENNIT
                     LTIYDYNHALRLNETAKKLNKQAKIHIKVETGMNRLGFLPTAENADKIADIAKFENIK
                     IEGAFSHQAKADEKDKTTAHMQADRFIKFIGMLEERNVQIPIKHIANSATIIELPEYY
                     FDMVRPGIILSGFYPSDEVDTEKYKFKICVTLKAKVANVKTIEAGEGVGYGHLYRADK
                     PVVVGTIPLGYADGYSRLLSNKGYVVVKGVKCPILGKVCMDQFMVDLSKVENPQIGDV
                     AIVYGDGTDGAMTAEDVANMRGTISYEVLTNLAVRRLPKIYV"
     gene            4765..>5896
                     /gene="glyA"
                     /locus_tag="PLD67_02755"
     CDS             4765..>5896
                     /gene="glyA"
                     /locus_tag="PLD67_02755"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOT21480.1"
                     /translation="MNFNLIEENDNELYRFIMEEKERQRINIELIASENFVSDTVLQA
                     MGSHLTNKYAEGYPGKRYYGGCEFVDKVENLAIERAKKIFGADHVNVQPHSGSNANFG
                     VYFAVLKPGDKILGMDLSHGGHLTHGSPVNLSGSYFNVVFYGVNRETETIDYDQIREI
                     AKKERPKLIVAGASAYPRIIDFKAFRDIADEIDSYFMVDMAHIAGLVAAGLHPNPVDY
                     ADFVTTTTHKTLRGPRGGMILCRQEFAQKIDKAIFPGTQGGPLMHIIAAKAACFKEVL
                     DDEFVLYQQQVIKNAKKLSEELMNKGFRLVSGGTDNHLMLVDVRNKNLTGKEAEKILD
                     EIHITTNKNTIPFDTQSPFITSGIRIGTPAVTTRGMKEAEMIE"
BASE COUNT         2142 a          846 c         1149 g         1759 t
ORIGIN      
        1 ggtcgctatg acttatcttt ttgttttttt gatgttatca attgttgtca agactattac
       61 accaaatata ataaacagta tttcagaatt atcccaagat ataccaagac tatacaaaaa
      121 ggcaaacagc atgttatcta ttttgagtga tgagctaatc gtaaaaattt atcttgccga
      181 aattgcacaa aaattatctt caattctgac agcaataata aataatgctc tcactaaagt
      241 catcaacata ttcatggcat ttgcaaatac attgttgtca ataattattt ctgtatatat
      301 tttaattgat aaagaaagta ttataaattg gtttaaagaa tttgccagcc tgtttatagg
      361 cagaaaagca gcaaatgata ttgttaggat aagccacatc ctgtatcgca atgtttcaag
      421 ctacatatcg ggaaaagcaa ctgcttctct cattattgca tttttaattt atataggctc
      481 aaaatatatt ataaaatgtc cttatcctgc aattgatgga atggttatag gaattaccaa
      541 tatgattcct tattttggca catttatagg gagtgttccg attgtgttga taaatgtttt
      601 atatactccg caaaaagggg ctcttttgtt tattttaatc ctaatattac agcaactgga
      661 gagtcttata atagacccta aaattttagg cagtcagttg tcaataaagc ccttattagt
      721 aattatttcg ataataatcg gaggaggatt gtttggtccc ttaggtcttt tttttgcagt
      781 tcctgttgca gcattgatta aatcaatagt tgatgtctat atgttgaaat ccctaaaaaa
      841 tgttcacatt attgataaat cttgatataa tttgtgagtt tgtggataaa actgttgaca
      901 agtcgcccaa aagccataaa acatgtgtat aagtattatt aataaagttg aaaaacagtc
      961 tggttttaaa gcagaaaaaa gcattgtaat gttaatgtaa cactaattaa ataaatataa
     1021 tgtataataa tattaaagaa atactattac tatgcatagt atttttttaa ttgaatgctt
     1081 taaggattga ttatatgaaa aaaacactat tggtgataat aattttaata tttgttgcat
     1141 cttttataaa tatatatgct gctgtctttc ctgatataaa aaaagatcct tatttagaag
     1201 aagcaatagg cgtattgtca agttataagg ttgtacaagg ctatccggat aacagcttta
     1261 agcctgatag aacagttact cgtgcagaaa tggcaaaggt tgtaactgta gcggcaggat
     1321 ttaatgaata ctccaaaaat atgacttcca gttatgaaga tatgcatggg cattgggctg
     1381 aaagttatgt tgagctggct gatgtattag gtattgttaa aggaatttct ccatcagtct
     1441 acggacctga taacctgata aagtttgaag aagcctatac aatgatttta aggctattag
     1501 gatataccga tgaatcactg atgggaagct ggccttctaa ttatcgtgaa aaagcaatag
     1561 aactgaattt atttgaaaat attaattcaa ataccgaatt tgcatctcga agagatatta
     1621 caataatgtt atttaatgca cttagcaaaa acttagtttc ggtaaaggaa aacaatactt
     1681 acaacgttac aaacaaaaca cttatttcaa agttaggcaa aatggaaaca agagtagtat
     1741 caatcaaaga cttaaatatt gataattttg actatacgga ttatttgttc aataagtggg
     1801 atatttatta tgataactat ggaaataccg tctatgtaag caacccccaa tataatgaat
     1861 tttcgggaac tgtaacaagc atactttcaa acagagtaat ctttgtaact gatgattacg
     1921 gcaatgtgag agtttttcag gttcctgata ttcccattat aattaacgga gaaagaggaa
     1981 gctttaataa tttaaatgaa tccagcgtaa aaatcatcta tgaagaagat tctttcaaag
     2041 gagaagttat aggtatcata gcttataaag caactgatgt tattgttgtc gatagagatg
     2101 acttgtataa ggaaggaagc agacagtttg ccggtaaaaa cctgcccttg agcaattcgg
     2161 aagttaactt taataaaatt gatattaaag gcgacgcaaa atctttggag gatataaaag
     2221 taaacgacgt ggtgtacttt tatgaaacac aaaatgataa aaactcctca ttgacactta
     2281 atgttttaag gtctaaggtt gaaggcgttg ttacaagtgt agacaatgta aatggtgcta
     2341 cattttatac cataaacaat ataagctata tgacaggaaa agattatatt tttacagaga
     2401 aggcaaatgt aaatgattca gttacattaa tattggataa aaacaataat attataaagc
     2461 tgtatataaa taaatattgc aaaaggccat cgacttacgg cattgtaatc aactcgtcaa
     2521 gcggcacaaa cggtaatgcc gaagcgcgaa tacttgatga atacggaagg attagaacat
     2581 attccttagc ggacaactca agtgttgtaa gagtgcatga aaatgattca agtgttttaa
     2641 agcaatcatt aataggaagc aatgatttag ttatgtttga ccctgtgtca aacgggacat
     2701 taaaaataat aaatttaatg caaaccagaa atattgcaag tccatacaat aatcaaacgc
     2761 aaaccttaga aaacggcggt tataaaatat catcagatac atttattgtt tacagaaaca
     2821 acggtcaata ccaattgtta gtaccttccc agctgggaga atacctggaa ggaagagcag
     2881 ttgttaatta tatgggacat attgatgcca tttatcttac taagggaata aaagaatcaa
     2941 atgtaataac aataacaccg gatacaccac aaaactacaa cggaaccata tacggcataa
     3001 taaagggtgt tacaaaatta gatgatacta caagccatat acagtttttt aacgacagca
     3061 acgtattttc tgtttcaaat acatctgttg cgggcaaaag tatttcatca atgatgaact
     3121 cctatgtaag agcagtaatt gttaacggta tggtttcaag cattgagaaa gtaactcctg
     3181 aaacatcaag aataaaaata tctcaaattt ataccaatca aatgctgatt gacaatataa
     3241 cgtatatgga atatgcacaa gacgtaaagg tctatgtttg taccttggat agttcgggaa
     3301 atataagcga ttttaaagaa ggtactaaat cagatattaa agccggctca acagcccagc
     3361 tttataacct gtacggcgga tttgacggag tgattgacgt tgtatttgtg tttaaataaa
     3421 aagtttcttc aggctgcgcc ttcagaatga caagattgta tactttgtca tcaaactggg
     3481 ggcgtatgct ttttttattg attaattgac tttggtttgc tataatttta ttaataatac
     3541 aagttgtgga atattcctct tagcttacct gcaaaggtta tcataggggg tgttctccat
     3601 accactaaag gaggagcaat taaatgaacg aaaagctgag acctgcctgg gtcgaaataa
     3661 acagagaaaa agccattcat aactttttgg aggtaagaag agcagtagga cctgacagga
     3721 aaatatgtgc tgttgttaaa gcagacagct atggaatggg agctttggaa ttatcaaaaa
     3781 tgtatcttga aaatggcgta gatatgtttg ccgtggcagt aatcggagaa gcttttgaac
     3841 tgagaaaaga aataaaagat aaagacattc tggtacttgg ttttacatca gaggaatttt
     3901 acgatgatgc aatagaaaat aatattacat tgactattta tgactataat catgcattaa
     3961 gattaaatga aacagcaaaa aaattaaata aacaagcaaa aattcacata aaagtagaaa
     4021 caggcatgaa cagattaggt tttttgccta cggcagagaa tgcggataag atagctgata
     4081 ttgctaaatt tgaaaatatt aaaattgaag gtgcattttc acatcaagct aaggccgatg
     4141 aaaaagacaa aacaaccgct catatgcagg ctgacagatt cattaaattt ataggtatgc
     4201 tcgaagaaag aaatgtgcaa atacctataa aacatattgc aaacagtgct actataatag
     4261 aattgcctga gtattatttt gatatggtaa gaccgggcat tattttatcg ggattttatc
     4321 cttctgacga agtggatacc gaaaaatata aatttaaaat ttgcgttaca ttgaaagcaa
     4381 aggttgcaaa cgtaaaaaca attgaagccg gcgaaggtgt cggatatgga catttgtacc
     4441 gcgcggataa gccggtggta gttggcacaa ttccgttagg atatgctgac ggatattcaa
     4501 gattgttgtc aaacaaggga tatgtggtag taaaaggagt aaaatgccct attttgggaa
     4561 aagtatgtat ggaccaattt atggtggatt tgtctaaggt ggaaaaccca cagataggag
     4621 atgtggctat agtatacggt gacggtactg atggagcaat gacagcggag gatgtagcca
     4681 acatgagagg tactatttct tatgaagtac tgacaaattt agccgtaaga agacttccga
     4741 aaatctacgt ttaaggagta ctatatgaac tttaatttga ttgaagaaaa tgataatgag
     4801 ttgtatcgtt ttataatgga agaaaaggaa agacaaagaa ttaacattga attaatagca
     4861 tcggaaaatt ttgtttctga tacggttttg caggcaatgg gaagtcattt aaccaataaa
     4921 tatgctgaag ggtatccggg caaaaggtat tacggaggat gcgaatttgt tgataaggta
     4981 gaaaaccttg caatagaaag ggctaagaaa atatttggcg cagaccatgt aaatgttcaa
     5041 ccccattcag gctcaaatgc aaactttgga gtatactttg cagtactgaa gccgggagat
     5101 aaaatattag gtatggactt atctcacgga ggtcatctta ctcacggaag tcccgtaaat
     5161 ttatccggct catacttcaa tgtagtgttt tatggagtaa acagggaaac tgaaaccatt
     5221 gattatgacc agataagaga aatagcaaaa aaagaaaggc ctaaattaat agtggcagga
     5281 gcaagtgctt atccgagaat tattgacttt aaagcattca gggatatagc agatgaaatc
     5341 gactcttact ttatggttga tatggctcat atagcaggac ttgtagctgc aggactgcat
     5401 cctaatcccg ttgactatgc agactttgtt acaacaacca ctcacaaaac cttgagaggt
     5461 ccaagaggcg gaatgatttt gtgcaggcaa gaattcgcac aaaaaattga taaggcaata
     5521 tttccgggaa cacagggagg tcctttaatg cacattattg ccgctaaagc cgcatgtttt
     5581 aaagaagtct tggatgatga attcgtattg tatcagcagc aagttataaa aaatgcgaaa
     5641 aagttatccg aagaacttat gaataaggga tttagattag tatccggcgg tactgacaat
     5701 catctgatgt tggttgatgt caggaacaag aacttaacag gcaaagaggc agaaaagata
     5761 cttgatgaaa ttcatattac tacaaataaa aatactattc catttgatac tcaaagcccc
     5821 tttattacaa gcggaataag aataggaact cccgcagtta ccacaagggg tatgaaagaa
     5881 gctgaaatga ttgaat
//
LOCUS       DAOSOW010000066         5851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_13799_length_5851_cov_4.024500, whole genome shotgun sequence.
ACCESSION   DAOSOW010000066 DAOSOW010000000
VERSION     DAOSOW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13799_length_5851_cov_4.024500"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..556)
                     /locus_tag="PLD67_02760"
     CDS             complement(32..556)
                     /locus_tag="PLD67_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT21481.1"
                     /translation="MVPWLHIFPSHLIDRGLNELFASKILGGVLFIMALSKILVGAVY
                     DRIGIKFAIGICLVSFVISSVLLLIVDSELIAWFFAALFGISLSSQTVLLPLFISAFA
                     GDEYLSDIIGIASALAGAGIAIGTPIINLTYDLTGSNDMILIAFTILGIFNMLLTIIA
                     LKKAKNSEIPRTDI"
     gene            complement(683..1246)
                     /locus_tag="PLD67_02765"
     CDS             complement(683..1246)
                     /locus_tag="PLD67_02765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT21482.1"
                     /translation="MDKKFNYGYVVAVLCFIIAFFYVGSAYNTGSLYVVPVTNHYGFA
                     RAEFSLIFSIISIFMIFASLSFVRLYRRLGIKNLVILGAILCVLAFFVYYKSTNLIQF
                     YIGAVLFGIGITYTNLMTFALLINSWFTENRGTILGLISAGSGFGGSVMSPVLGYLIL
                     TYGFKQAYLLTSIILFVLIIPIALCMK"
     gene            complement(1287..1988)
                     /locus_tag="PLD67_02770"
     CDS             complement(1287..1988)
                     /locus_tag="PLD67_02770"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOT21483.1"
                     /translation="MKYTIEKSVFELNPNIKFGILIGNNIRNSETRPEDEERLRRAEA
                     KFRNKVKPDEIRDLINVKLYREVMKNSGINPNKYLPSVEAMFKRIAKGGQLPVINALV
                     DLCNAVSIERGISLGGHDLKDIHDDLEVRYSRKGDVFLPFGAENYEDVEEKELIFTSG
                     NIVQTYKWLWRQSELGKLTLESTDVFFQLVGFDYEEGSSLYDAMSDIEYLVINRFHGT
                     CRKYLVDISNPSIEF"
     gene            complement(2059..2928)
                     /locus_tag="PLD67_02775"
     CDS             complement(2059..2928)
                     /locus_tag="PLD67_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HOT21484.1"
                     /translation="MKRSDGYLLKDLKPFEKIIPYVMEKRSDAQNFSKQIFPAESIDK
                     YILEKKQSGYSFSYMHVFIAAYVRVIAERPQLNRFVMNNQIYQREKIYVSMAVKRSLR
                     DDGEETTVKFEFTGKETIFEIAEIIDKDIARALSYSEDNNTDKLAERVMSMPGFVKKL
                     LVRTLKAMDKYNLLPKSIIDASPFHTTMFFTYLKSIDTQYIYHHLYDFGTTGLFAALG
                     KTVKIPIVENDQVVVKKCCEIGYTMDERICDGVYYARSFKLFEKYISNPRLLESELIQ
                     KVPKDQKVKKAFA"
     gene            3153..4487
                     /locus_tag="PLD67_02780"
     CDS             3153..4487
                     /locus_tag="PLD67_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT21485.1"
                     /translation="MDYINPLGTEKISKLLFKFSIPAITGMMINALYNVVDRIYIGNS
                     PDIGTDGIAGITVGFPIMIVFLALGVLFGVGGATNFSINLGRKNYTEAENYLGNTFSL
                     LILAGVLFAIIVRINLETILLFFGASEAILPYAMEYMKIIAFGAPFHIVSIGMNHFIR
                     ADGNPKLAMYTMFLGAGTNIILDPIFIYGFKMGMAGAALATIISQFFSFLWVTSYFIG
                     KHSRNKLKIEYLRLKSNLTGKITTLGLPGFSLQLATSALNAILNKNLVFYGGDIAVSG
                     MGVINSIQTLLIMPIIGLNQGVQPIISYNYGAKNYERVKDAAKLAIKAATIIVSFGFI
                     ITRLFPVQLISLFNRNTELIKFGKSAIFAWFLCFPVVGFQIVGSNFFQAIGRPKSAML
                     LTLTRQLILLTPAIIIFPRLWGVEGILYAAPFADFLSFLLTATFFYLGIRKL"
     gene            complement(4561..5745)
                     /locus_tag="PLD67_02785"
     CDS             complement(4561..5745)
                     /locus_tag="PLD67_02785"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOT21486.1"
                     /translation="MISQEMIKLGTQRSVIRELFSYGLQRAEVVGAENVLDFSIGNPS
                     VPAPKEVQEAIIDIVKNNDPMVYHGYSTSQGHAGCRKAVAGNLNKRFNKSYTLNDIFM
                     TCGAAASLCITFRALTASADDEIIVLAPYFPEYKVFVEGQGAKMKLVQSKNDMRLNLE
                     GIKEAVNKNTKALLINSPNNPSGVVYSKEEIKELSDYLKKMQNELGQTIYLISDEPYR
                     ELVFNDEPVPFIPDYYDNTIICYSWSKSLSLPGERIGYVLVNDKCEDNKTVMAAVGGA
                     ARSLGYVCAPTLFQLVIEKCIDVKPNLEVYRKNRDILYDALTEMGYNVAKPDGAFYLF
                     IEAPNGDSKEFSDKAKEHDVLVVPGTGFGTDTHIRLSYCVDTEKCEKALIVFEKLIKE
                     IK"
BASE COUNT         1888 a         1115 c          889 g         1959 t
ORIGIN      
        1 aagaatagtg gtaaggaatt ttattctgaa ttcaaatgtc cgtcctcgga atctcggaat
       61 tctttgcttt ttttaatgca ataatagtaa gcagcatgtt aaaaatacca aggattgtga
      121 atgcaattaa aatcatatcg tttgaaccag ttaaatcata tgtaagatta attataggag
      181 ttccaatagc tatacctgct ccagccaaag cagatgctat cccgataata tcacttaaat
      241 attcatcccc tgcaaaagct gaaatgaaca agggtaataa cactgtttga gaacttaatg
      301 aaatgccaaa taatgcagca aaaaaccacg ctattaactc actgtcaaca ataagcaaaa
      361 gaaccgagct tattacaaaa gatactaagc aaataccgat agcaaactta attccaattc
      421 tgtcataaac agcaccgact aaaatctttg acaatgccat aataaataaa actccgccta
      481 atattttaga agcaaataat tcatttaatc ccctgtctat taaatgagac ggaaaaatat
      541 gaagccatgg caccattgag aaacccatta aaaatataac ggcaagacct attattacac
      601 tttgctcttt taataactgc ccggttgttt ttttcttaat atttaatttt tcttcactat
      661 ttgttgcata tccgacaggg ttttatttca tacataaagc aatcggaata atcaaaacaa
      721 acaaaataat cgaagttaat aaataagctt gtttaaagcc gtaagtcaat ataaggtaac
      781 ctagtacagg actcataaca gacccgccaa atccgcttcc tgcagaaatc aaacctaaga
      841 tcgtccccct gttttcagtg aaccagctat ttataagcag ggcaaaggtc atcaaatttg
      901 tatacgttat tcctattcca aacaagacgg cgcctatata aaattgaata aggttagtcg
      961 atttgtaata aacaaagaaa gccaaaacac ataaaatggc tcccaagata actaagtttt
     1021 tgatacctaa gcgtctgtat aatctcacaa aagaaagact ggcaaaaatc atgaaaattg
     1081 atataatact gaaaatcaac gagaattcag ctctggcaaa accatagtga ttggttaccg
     1141 gaacaacata cagactgcct gtattataag cactcccaac ataaaaaaat gcaataataa
     1201 agcataatac tgctacaaca tatccatagt taaatttttt gtccataaaa actcctttgc
     1261 aaaaaaatgc tgcctaaatt gaagatttaa aattctatgg atggattgct tatatctaca
     1321 agatatttcc tgcaggtacc atgaaacctg ttaattacta aatattctat atcgctcata
     1381 gcgtcataaa gggatgagcc ttcttcataa tcaaacccta caagctggaa aaatacgtca
     1441 gtgctttcaa gagtaagctt tcctaattcg ctttgcctcc aaagccattt atatgtctgg
     1501 actatgttgc cgcttgtgaa tatcagctcc ttttcttcaa catcctcata gttttcagcc
     1561 ccgaaaggta aaaacacatc tccctttctg ctgtaacgca cctctaaatc gtcatgaata
     1621 tcttttaaat catgcccgcc taaggatatt cctctttcta tggatactgc attgcataaa
     1681 tcgactaagg catttataac cggcagctgt ccgccttttg ctattctttt gaacattgct
     1741 tctacggaag gaagatattt atttggatta atacctgaat ttttcataac ttctctgtac
     1801 agcttcacat ttatcaaatc cctaatttca tcaggcttaa ctttatttct gaatttggct
     1861 tctgctcttc tcagcctttc ttcatcctca ggccttgtct ctgaatttct aatgttattg
     1921 cctattaata ttccgaattt tatattagga tttaattcaa acacagactt ttcaattgta
     1981 tatttcattt tttaactcct cttagttatt ctgcctctct accatagaga ctgctatttt
     2041 tccacgtccg gattcttatt aagcaaatgc ctttttaact ttttggtctt ttggaacttt
     2101 ttgaataagc tctgattcca ataatctagg attgctgata tatttctcaa agagcttaaa
     2161 ggaccttgca taatacacac cgtcacatat tctttcatcc attgtatagc ctatttcgca
     2221 gcatttttta acaacaacct ggtcattctc tactatcgga atcttcactg tcttgcctaa
     2281 tgcagcaaat aagcctgttg ttccaaaatc ataaagatga tgatagatat actgagtatc
     2341 tattgacttt aagtaagtaa aaaacattgt ggtatgaaag gggctggcat caataatact
     2401 ttttggaagt aagttatact tatccatcgc ttttagagtt ctaaccaata atttcttcac
     2461 aaacccgggc atcgacatta ctctttcagc aagcttgtcg gtattattat cttcactgta
     2521 ggataatgcc ctggcaatat ctttatctat aatttcagca atttcaaaaa tagtctcttt
     2581 tccggtaaat tcaaatttta cggttgtttc ttccccgtca tctctaagtg aacgttttac
     2641 agccattgaa acataaattt tttctctctg ataaatttga ttgttcatta caaaacggtt
     2701 aagctgcggt ctttctgcaa tcactcttac gtaagcggct atgaaaacat gcatgtaact
     2761 aaaactataa ccgctttgct ttttttctaa aatatattta tcaatacttt ccgccggaaa
     2821 aatctgctta gaaaaatttt gagcatcgct tcttttttcc atgacataag gtattatttt
     2881 ctcgaaaggt ttcaagtctt ttaataaata tccgtcactt cttttcatac atcctccata
     2941 tactaatcta aattctgcaa ttcaaggagc tcatataata catagcggtg cgtaccggaa
     3001 aaaaacattt tccggaacac cgcttaatcg aagaataaca caaatttgca ttattatcaa
     3061 ttgttttatt ttttattgct caaatcttct tgtttcaata tataaaaatt tatagtataa
     3121 tatcactatt aataacataa acggaggcaa aattggacta cataaatcca ttaggcacag
     3181 aaaaaatatc aaagctttta tttaagtttt caatacctgc tattacgggc atgatgataa
     3241 acgcattata caatgttgtt gacaggatat atataggcaa cagccctgat ataggtactg
     3301 acggaattgc aggaattacg gtaggatttc ccataatgat agtattcttg gctctgggag
     3361 ttttatttgg ggttggaggt gccacaaact tttcgataaa tttaggaaga aaaaattata
     3421 cggaagccga aaattattta ggcaatacat tttcactgct tattttggca ggagtactct
     3481 tcgcaataat agtgcgtatt aacttagaaa ctatattgct tttctttggt gccagcgaag
     3541 caatccttcc ttatgctatg gaatacatga aaataattgc attcggagca ccctttcaca
     3601 tagtaagtat tggtatgaac cattttatac gagctgacgg aaatccgaaa ttagccatgt
     3661 atactatgtt tttaggggca ggaactaata ttattttaga tccaatattt atttatggtt
     3721 ttaaaatggg aatggcagga gcagctttgg caactattat ttctcaattt ttctcattct
     3781 tatgggttac ttcatatttt atcggaaaac atagcagaaa taaattaaaa attgaatatt
     3841 tgaggcttaa atcaaatttg acaggtaaaa ttacaacctt aggtctgccc gggttttctc
     3901 ttcaattggc aacaagtgct ttaaatgcaa tacttaataa aaatttagta ttttacggcg
     3961 gtgatattgc tgtttccgga atgggagtta taaacagcat acaaactctg ctgataatgc
     4021 cgattattgg cttaaaccaa ggtgtccagc caattatcag ctataactac ggtgccaaaa
     4081 attatgaaag agtaaaggat gcagcaaagt tagcaataaa ggcagcgaca ataattgttt
     4141 cctttggatt tattattaca aggttgtttc ctgtacaatt gatatcatta tttaacagga
     4201 atacggaatt aataaaattt gggaaatctg caatatttgc atggttttta tgtttccctg
     4261 tagtaggctt tcaaatagta ggttcaaact tctttcaagc tataggcaga cctaaatctg
     4321 caatgttatt aacccttaca aggcagttga ttttgttaac gcctgctatc ataatattcc
     4381 ctagactctg gggtgtggaa gggatactat acgcagcacc tttcgcagat ttcttatcat
     4441 ttttgttaac agcaacattc ttttatttag gaataagaaa actataatta aaattgtaaa
     4501 tgacgatgga agaagtagaa atgaagaatg gcgcatagcg ccattcttaa ttatattata
     4561 ttatttaatc tctttaatta acttttcaaa tacaatcaat gctttttcac atttttcggt
     4621 atccacacaa tacgaaagtc ttatatgtgt atctgttcca aagccggttc ccggtactac
     4681 caagacatca tgttcttttg ctttatccga gaattctttt gaatctccat ttggggcttc
     4741 tatgaataaa tagaacgcac catcaggctt ggcaacattg tatcccattt ctgtcaatgc
     4801 atcataaaga atgtctctgt ttttcctgta tacttccaaa tttggtttga catcaataca
     4861 tttttcaata acaagctgga aaagtgtagg agcacataca taaccaagag accttgcagc
     4921 tcctcccaca gcagccatta cagttttgtt atcctcacat ttatcgttta ctaagacata
     4981 ccctatacgc tctccgggca atgataatga ttttgaccat gagtaacaaa taattgtatt
     5041 gtcataatag tccggaataa aaggaaccgg ctcgtcatta aacaccaatt ctctgtaggg
     5101 ctcgtcggaa atcaaataga ttgtctgccc cagttcattt tgcattttct ttaaataatc
     5161 tgacaattcc tttatttctt cttttgaata cactactcct gaaggattgt ttggtgaatt
     5221 tataagaagg gccttagtat ttttattaac agcttcttta attccttcta aattaagtct
     5281 catgtcattt ttgctttgaa ccaatttcat ctttgctccc tgtccttcaa caaaaacttt
     5341 atattcagga aaataagggg ccaagacaat aatttcatca tcagcacttg ctgtcaaagc
     5401 tctgaaagta atacataatg aagctgcagc gccgcaagtc ataaatatat cattcaatgt
     5461 ataggatttg ttaaatctct tgtttaaatt tccggccact gctttcctgc agccggcatg
     5521 tccttgcgat gtgctgtatc cgtgataaac cattgggtcg ttattcttca caatgtcaat
     5581 gatggcttct tgcacttctt tgggagccgg tacacttggg tttcctatac tgaaatccaa
     5641 aacgttttct gctccgacaa cttctgctct ctgcaaacca tacgaaaata attctctgat
     5701 aacagatctt tgcgtgccta atttaatcat ttcttgtgat atcatattat cctccaataa
     5761 tttgtaatat ttcctttcca ctatattacc atattaatag ataaaagtaa atgttataaa
     5821 atatcagtcc ttcaggaaaa atatagtcaa a
//
LOCUS       DAOSOW010000067         5846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_13815_length_5846_cov_4.229321, whole genome shotgun sequence.
ACCESSION   DAOSOW010000067 DAOSOW010000000
VERSION     DAOSOW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5846
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13815_length_5846_cov_4.229321"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..590
                     /locus_tag="PLD67_02790"
     CDS             81..590
                     /locus_tag="PLD67_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012635045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HOT21487.1"
                     /translation="MLCNECGKNEANVHLTHIINGKKTESHLCEECAKKNQTIFNPNF
                     SMENLFSAMLNNSFNNKTYLPAKGCSNCGMTYENFRTTGKFGCSQCIDSFKGRLKPVI
                     KSIQGYDRHTGKIPKRTGGAYKVQMDIERLKNELRCAVEREEYELAAELRDKIYELEK
                     KKGDEQFEK"
     gene            580..1566
                     /locus_tag="PLD67_02795"
     CDS             580..1566
                     /locus_tag="PLD67_02795"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015310871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HOT21488.1"
                     /translation="MKNNDIVITSRIRLARNLKNYKFPIYMTESESKSVINEIYNAIS
                     KHENYKLTYMKDLSDVKKNSLIENHIISPALANKRNEGAFLLSPDGKTSVMINEEDHI
                     RIQTIEDGLSLKECWKLSNEVDDIIEESVDYAFDRELGYITACPTNIGTGMRASVMLH
                     LPALSITNQIDKLLYGVSQLGVAVRGVYGEGTKSIGHLYQISNQGTLGASEETLIDKI
                     NQIVMQIIDKEKNTREHLMKNNYDDLEDDFYRAYGLLTNARKMTVDEAMKLLSLIKLG
                     SEMGITPMVKGKNIYQLMIWIQPNNISTIDTELSPKDRDKKRAEIIRRELLR"
     gene            1597..4035
                     /locus_tag="PLD67_02800"
     CDS             1597..4035
                     /locus_tag="PLD67_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOT21489.1"
                     /translation="MMGGLNNSARNALALAQEEVKNFKQNVLGTEHILLGLMIEEESI
                     AGKILRDKLDIDKVREIIKNSTGMGDSVPEYITISPRSKFIIELARQYAAQLKMPYVG
                     CEHILLGLIEEGEGIGAQIIKSVISLKDLKNEVLMAMQGAGGAEPGNQDIGGSMAPQT
                     SNTKTIDKYGTDLNQRAKEGRLDPVIGREDVIERVIQILSRRTKNNPCLIGEPGVGKT
                     AIAEGLAQEIIKGSVPETLKDKRVITLDMAGMVAGAKYRGEFEERLKNAIDEIKAAGN
                     IILFIDEMHTIIGAGAAEGAIDASNILKPVLARGELQVVGATTLDEYKKHIEKDAALE
                     RRFQPIIVEEPTVEDTIRILKGLRDKYEAHHKVAITDEAIEAAASLSHRYISDRFLPD
                     KAIDLIDEAASRIRLKSSTAPKELKELEDKIKEVDTEKRTAINNQDFEKAASLRDEEK
                     KLTDELNKQRKLWAAKNIKDIKINYEDIADVVSQWTGIPVKKLQGDESERLLNMEEIL
                     HKRVIGQEPAVEALSKAIRRSRVGLKDPKKPIGSFIFLGPTGVGKTELSKALAEAMFG
                     DEDAMIRIDMSEYMEKHSVSKMIGSPPGYVGFDDGGQLTERVRRKPYSVILFDEIEKA
                     HPDVFNILLQILDDGRLTDAKGRTVDFKNTVVIMTSNVGASTIKKQKTLGFATNASDE
                     EKDEYEKMKENVLNELKKTFRPEFLNRIDEIIVFHSLNKEHIKQIVSLMINNLSKRLD
                     EMNIHIKVDEKAMDILADAGFDPVYGARPLQREIRRRIEDKLSEELLKGTIKKSDTIL
                     ISADDKELIFSNDK"
     misc_binding    4090..4339
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4381..>5846
                     /gene="aspS"
                     /locus_tag="PLD67_02805"
     CDS             4381..>5846
                     /gene="aspS"
                     /locus_tag="PLD67_02805"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005352405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOT21490.1"
                     /translation="MLRKNEYRTHTCGELRESDIGKHVTVSGWLENVRDHGGVKFADL
                     RDQYGIVQVVVEDETMLDGIPKESAIRVTGFVAERDEDTINKKLDTGYIEIEAESITV
                     LGKVKEFLPFEVTSSKETKEDLRLKYRFLDLRNVEVQKNIILRSKVISFMRSKMTEMG
                     FLEIQTPILTSSSPEGARDYIIPSRKHPGKFYALPQAPQVFKQLLMVSGFDKYFQIAP
                     CFRDEDARADRSPGEFYQLDFEMSFATQEDVFSVAEEVLYDTFTKFSDKQVSKPPFKR
                     IPYEESMLIYGTDKPDLRNPLTIIDISDMFEETDFAPFRKKTVRAINVPECSSQPKRF
                     FEEMLEFATKIGMKGLGYIKVNDEMEYQGPIDKYLSHEDRKELAERARLKPGDVLFFI
                     ADTREEAPKLAGQIRAELAKKLKLIDENRYEMCYIVDFPMYEIDEKTGGIAFTHNPFS
                     MPQGGLKSLMDKDPLDIKAFQYDIVCNGIELSSGAVRN"
BASE COUNT         2205 a          875 c         1279 g         1487 t
ORIGIN      
        1 agctgcgggc cgacctttta aaggaaatga tattggtatc cgtagagaat aatgattatt
       61 gatttatagg aggggtataa atgctttgta acgagtgcgg taaaaatgaa gccaatgttc
      121 atttaactca cataattaac gggaaaaaga cagaaagcca tttgtgtgaa gaatgtgcaa
      181 aaaagaacca gacgattttt aatcccaatt tttcaatgga gaatttgttc tctgcaatgc
      241 tgaacaattc ttttaacaat aaaacatact tacctgcaaa gggatgctca aactgcggca
      301 tgacatacga aaacttcaga actacgggaa aattcggatg cagccaatgc attgattcat
      361 tcaagggcag gttgaaacct gttataaaaa gtatacaggg atatgacaga catacaggga
      421 aaattcctaa acgaacaggc ggagcttaca aggttcaaat ggatattgaa agattgaaaa
      481 atgagttaag atgtgccgtt gagagagagg aatatgaact tgcggcggaa cttagagata
      541 aaatctacga attagaaaag aaaaaggggg atgagcagtt tgaaaaataa tgatatcgta
      601 ataacgagca gaatcagact tgcaagaaat ttaaaaaatt ataaattccc tatatatatg
      661 accgagtcag aatcaaaaag tgtaattaat gaaatttata atgcaataag caaacatgaa
      721 aattacaaat taacatatat gaaagactta tcagatgtaa agaaaaattc attgatcgaa
      781 aaccatatta tctctccggc attggcaaat aaaaggaatg aaggtgcatt tcttttaagc
      841 cccgatggaa aaacttcagt tatgataaat gaggaagatc acataagaat tcaaactata
      901 gaagacggat tatccttgaa ggaatgctgg aaattaagca atgaagtgga tgatataatt
      961 gaggaaagtg ttgattatgc atttgacagg gaattaggat atataactgc ctgccccact
     1021 aacataggaa caggtatgag agcatctgta atgctgcatt tgcctgcact ttcaataaca
     1081 aatcaaattg acaaactcct gtacggggtt tcccagttag gtgttgcagt gagaggagta
     1141 tatggtgaag gaacaaaatc gataggacat ttgtatcaaa tttcaaacca gggaacattg
     1201 ggtgcttccg aagaaacatt aattgataaa atcaatcaaa ttgtaatgca aattatcgat
     1261 aaggaaaaaa acacaagaga gcacctgatg aagaacaatt atgacgattt ggaagatgac
     1321 ttttacaggg catacggatt gcttacaaat gcaagaaaaa tgacagtgga tgaagcaatg
     1381 aagttattgt cgctcataaa gcttggaagt gaaatgggaa taactccaat ggttaaaggc
     1441 aagaatatat atcagcttat gatttggatt cagccaaaca acatttcaac tattgatact
     1501 gaactttcac caaaggatag agacaaaaaa agagcagaaa taataagacg tgaattattg
     1561 cgttgaaata tataatacaa aaaggagatg aaagatatga tgggaggact aaataattct
     1621 gcaagaaatg ccttagccct tgctcaggaa gaagtaaaaa atttcaaaca aaatgtactt
     1681 ggaaccgagc atattctatt agggttaatg attgaagagg aaagtattgc aggaaaaatt
     1741 cttagagata aattagatat tgataaagtt agagaaataa ttaaaaattc aacaggcatg
     1801 ggtgattctg tacctgaata cattactatt agccccagaa gcaaatttat aatagagctg
     1861 gcacgtcagt acgcagccca gcttaaaatg ccttatgtag gttgtgagca tattttattg
     1921 ggacttattg aggaaggcga aggtatagga gcacaaatta taaaatcagt gataagttta
     1981 aaggatttaa aaaatgaagt gctgatggca atgcaaggcg caggcggagc ggagcccggc
     2041 aatcaggata taggcggcag tatggcaccg caaacatcaa atactaagac tattgataaa
     2101 tacggcactg atttaaatca gcgtgcgaaa gaaggaaggc ttgacccagt tatcggaaga
     2161 gaagatgtta ttgaaagggt aattcaaatc ttatcgagaa gaactaaaaa caacccctgc
     2221 cttattggtg agcccggggt cggaaagacg gcaattgcag aaggtctggc tcaggaaatt
     2281 atcaaaggaa gcgttcctga aacactgaag gataagcgtg taatcacctt ggatatggca
     2341 gggatggtgg caggagcaaa atatagagga gaatttgagg aaagactaaa aaatgccatc
     2401 gatgaaataa aggcagcagg aaatattata ttatttatag atgaaatgca tacaataatc
     2461 ggtgccggag ctgccgaagg ggcaatagat gcatccaata ttttaaaacc cgtattggcg
     2521 agaggggaac ttcaggttgt aggtgcaact accttagatg aatataaaaa acatattgaa
     2581 aaagacgctg ctcttgaaag gagattccaa cctataattg ttgaggagcc gacagttgaa
     2641 gacaccataa gaattcttaa gggattaaga gataaatacg aagctcatca caaggtggca
     2701 atcactgatg aagcaattga agcggcggca agtctttctc acagatatat ttccgacaga
     2761 ttcctgcctg acaaggcaat agacttaata gatgaagcag cttcaaggat aagattaaaa
     2821 tcatcgacag ctcccaagga attaaaagaa ttggaagata aaattaaaga agttgacact
     2881 gaaaaaagaa ctgcaataaa caatcaggat tttgaaaagg cagcatcgct gagagatgaa
     2941 gaaaagaagc tgacggatga acttaataaa cagagaaaac tttgggctgc aaaaaacata
     3001 aaggatataa aaatcaatta tgaggatatt gccgacgttg tatcccaatg gacaggtatc
     3061 cccgttaaaa aacttcaggg agatgaatcc gaaagactcc tgaatatgga ggaaattctt
     3121 cataaaagag taataggtca ggagccggct gttgaagcat tgtcaaaagc aatcagacgc
     3181 tcgagggtag gacttaaaga cccaaaaaaa ccaatcggtt catttatatt cttaggacct
     3241 acgggagttg gaaaaacaga gctctcgaaa gccttggcag aagccatgtt tggtgatgaa
     3301 gatgcaatga taagaattga catgtccgaa tatatggaaa agcattcggt atcaaaaatg
     3361 ataggctctc ctcccggata tgtaggcttt gatgacggag gtcaattaac cgaaagagta
     3421 agaagaaaac catattcggt aattttgttt gatgaaattg aaaaagcaca tccggatgta
     3481 tttaatatct tgcttcaaat acttgatgac ggccgtttga ccgatgcaaa gggaagaact
     3541 gtagacttta aaaacacagt tgttattatg acatccaatg ttggagcaag caccattaaa
     3601 aagcaaaaaa cactcgggtt tgcaacaaat gcttcagatg aagaaaaaga tgaatatgaa
     3661 aaaatgaagg aaaatgtatt gaatgaatta aagaaaacat tcagacctga gtttttaaac
     3721 cgtattgacg agataatagt gttccattcg ttgaacaagg agcatataaa acaaatagta
     3781 tcacttatga taaacaatct gtcaaaacgt cttgatgaaa tgaatattca tattaaagtc
     3841 gatgaaaagg caatggatat actagcagat gcaggatttg acccggtata cggcgcaaga
     3901 cctcttcaaa gagaaatcag aagacgaata gaagacaaat tatctgagga gcttctaaaa
     3961 ggaacaatca agaagtcaga tacgatttta atttcagcag atgataaaga attaattttc
     4021 agcaacgata aataaaaaaa ctcttgacaa aagttgtagt ttgtatataa taattaaaaa
     4081 tgtagaaaat actcagcaat gatgggaaga gtaatttata attagagtca cagagagata
     4141 gcgccggtga gagtctatta caagattata aacgaagaac atccctgagc ttttgaccca
     4201 aattgcatat gcaagagtag gctcaaacgc atccgagcga taaagcggca aagacatgtc
     4261 agtgtctttt gagagagcct atatgtaagt atatgctaat tagggtggca ccgcgaaatt
     4321 caactccttc gtcccttgtg gatggaggag tttttatttt atatgaaagg attaataatt
     4381 atgttaagaa aaaatgagta ccgtacacac acctgcggag aattaagaga aagcgatata
     4441 ggaaagcatg tcacagtatc aggatggctt gaaaatgtga gggaccatgg aggtgttaag
     4501 tttgcagact tacgagacca atatggcatt gtacaagttg ttgttgaaga tgaaacaatg
     4561 ttggatggaa ttccgaagga aagtgcaatt cgtgttaccg gttttgttgc tgaaagagat
     4621 gaagacacaa taaataaaaa gcttgatacg ggatatattg aaatcgaagc agaatctata
     4681 accgtactgg gtaaggtcaa agaatttctt ccttttgaag ttacatcatc aaaagagaca
     4741 aaagaagatt tgagattaaa atacaggttt ttggacttaa gaaacgtgga agttcaaaaa
     4801 aatatcattc tacgctcaaa ggtaatatct tttatgagga gcaaaatgac agaaatgggt
     4861 tttttggaaa ttcaaacacc gattttaaca tcttcttctc ctgaaggagc aagagactat
     4921 attataccaa gcagaaagca tcccggaaaa ttttatgcat tgcctcaagc tcctcaagtg
     4981 tttaagcagc ttttgatggt ttcggggttt gataagtatt tccagatagc tccttgtttc
     5041 agagatgagg atgccagggc tgaccgctct cccggtgagt tttatcagct agattttgaa
     5101 atgtcatttg caactcaaga ggatgtattt tcggttgctg aagaagtatt gtatgatacc
     5161 tttactaagt tttccgataa gcaagtatca aaacctcctt ttaaaaggat tccttacgaa
     5221 gaatcaatgc ttatatacgg cactgacaaa ccggatttga gaaacccgct gacaataatt
     5281 gatataagcg atatgtttga agaaacagac tttgcaccat tcagaaagaa gacggtaaga
     5341 gcaatcaatg tgcctgaatg ctcatcccag cccaagcgtt tttttgaaga gatgcttgaa
     5401 tttgcaacta aaatcggaat gaagggactt ggctacatca aggttaatga tgaaatggaa
     5461 tatcagggtc ccatagataa atatctttct catgaagaca gaaaggaact tgctgaaaga
     5521 gcaagactga aaccgggaga cgtattgttt tttattgcag atacaagaga agaagcgcct
     5581 aaacttgcgg gacaaatacg agctgagctt gcaaagaaac tgaaattaat tgatgaaaac
     5641 aggtatgaaa tgtgttatat tgttgatttt ccaatgtatg aaatagatga aaaaacagga
     5701 ggaatagcat ttacacacaa cccgttttca atgcctcagg gaggattgaa atcattaatg
     5761 gataaagacc ctcttgacat taaagctttt cagtatgata ttgtatgtaa cggtattgaa
     5821 ctttcatccg gagctgtaag aaatca
//
LOCUS       DAOSOW010000068         5821 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_13885_length_5821_cov_4.275408, whole genome shotgun sequence.
ACCESSION   DAOSOW010000068 DAOSOW010000000
VERSION     DAOSOW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5821
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13885_length_5821_cov_4.275408"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            382..645
                     /gene="rpsT"
                     /locus_tag="PLD67_02810"
     CDS             382..645
                     /gene="rpsT"
                     /locus_tag="PLD67_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOT21491.1"
                     /translation="MANIKSAKKRILIINKKTQANKSRKSEIKTYIKKFETALSEGNY
                     EKAKEYLKICEKKLYQAAAKNTIHKNAAARKVSRLTKKFNKVA"
     gene            complement(669..1709)
                     /gene="holA"
                     /locus_tag="PLD67_02815"
     CDS             complement(669..1709)
                     /gene="holA"
                     /locus_tag="PLD67_02815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOT21492.1"
                     /translation="MSFNLIKNMADKNKLPNCLLLYGNEEYFIDYSIKYIKYKYIDKN
                     YEDMNYAEFEKPASLKDYFEFAYTFPFMSEKKLCVIKEAGFFTSTGSLDKKEEDRLLN
                     FIDENETCISIFVIKDGKADSRKKIVKKLKDNKAVFEFNRLTEGELTKYITEEFKKNN
                     FKISMSDANYMANNTGYLEYESTVSLYHVNNEINKIMSYNKEKKNITTSDLDLLMVKS
                     IESNIFRLVDCICENDKKSSFEILNEMLLNNTPEQYIFHMITRQYRMLYQYVMLQKKG
                     YSYNEIMSKMKIKNFVASKLAKQSKNLNMETIEYYMKRFLEIDSKIKTGEIDSRVGLE
                     LISNGIIGTGTN"
     gene            complement(1699..4014)
                     /locus_tag="PLD67_02820"
     CDS             complement(1699..4014)
                     /locus_tag="PLD67_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOT21493.1"
                     /translation="MYIIISIFTAYAFGIISYELNLYGYYFTALLAILIYNSLINKKY
                     MYNAVIIAFVILSYINCNYNSSSVLSQYKNESIVVEAKVKKQKKSGSESYYASVIGIN
                     NTPILNEENIILYTGKYKNFQENSIIKARVNVAGSQLNKNKMLFNYENYLRSKKINAT
                     VFADEDAYVIDENYSFLNNISSKFRKYTEKTFYYNLNKKNAEIILSIILGDVNYLEEE
                     LYDNIKVMGLAHIFAVSGTHIVLMYIFLLNVFKFVLSRRFSWLLSWCIIWFYGFLIGF
                     PLSIMRALVMFTLLFGSEVLYRKYNSLNSIGLAALVLTIYNPFWIFDAGFLLSFSAAL
                     SFIIYGKYIKTKTTVLRTVYMYLFLQIFTLPVIVYYFNFVPVMGVLYNLLLLPVFTFI
                     MIYGFILLILNSFAHIILIIPFNIYDYILHSLRYIIDISDKSAFNGLIMPTLSLCQTI
                     FFYITLFYMIYIHNNKKCNCKKIGIFAIVSLYSITYIAFPMMDDSLYLNIADVGQGLF
                     TTIKYKGLNMICDCGSTTNKQMGEYIAVPYLTKRGIAQVDGIFISHWDEDHYSGLNDI
                     LNSHIKVNNIFSSNSNENYTNVQKLNAGCQMKYDDNLSMEILWPYKDLSLNNTNNSSL
                     VISLNYKGRRILLPGDIEEEAEYAIYNGLNAYDIVLVPHHGSNTSSTVQFVDAVRPKI
                     AVLSYGRNFYGIPHEEVISRYRNTGSKIFSTFEDGEINIVLKDNNLYYNTYMNEKSDN
                     YYKLYLIGIIYKLSIFCFLLFWMLKGESYEL"
     gene            4081..5073
                     /locus_tag="PLD67_02825"
     CDS             4081..5073
                     /locus_tag="PLD67_02825"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOT21494.1"
                     /translation="MFINEYEVNNITDNPYLNEIYKEAVFIDIETTGLSSIFSDIISI
                     TFLLYKDDKYRIYQIFCQYKQDQPDALKYLNELIKLKKYMVTFNGNSFDIPFLAEKAK
                     QLNITLVFDSFNKIDLYNCIKKFRYKINTVDLKLKTVEKYFSIDRNDTLDGKDVITLY
                     EAYRLEQRKEFSYLILKHNYEDVYNLPFVMNNIFSLYDFVIYTKNLIITINNQDITIK
                     KNSIKCKFNILSLIAKDYINHSINYDMVLSVKPQTLKIDIPLNIYKDNNIREFYYLDN
                     DEYNIDNYTSIKGLKRNLIPIKLNNKMYYDNINRIVKEIVEEGFGCSEKQVPSF"
     gene            complement(5169..5241)
                     /locus_tag="PLD67_02830"
     tRNA            complement(5169..5241)
                     /locus_tag="PLD67_02830"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5206..5208),aa:Phe,seq:gaa)
     gene            complement(5262..5335)
                     /locus_tag="PLD67_02835"
     tRNA            complement(5262..5335)
                     /locus_tag="PLD67_02835"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5299..5301),aa:Ile,seq:cat)
     gene            complement(5354..5430)
                     /locus_tag="PLD67_02840"
     tRNA            complement(5354..5430)
                     /locus_tag="PLD67_02840"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5394..5396),aa:Ile,seq:gat)
     gene            complement(5469..5544)
                     /locus_tag="PLD67_02845"
     tRNA            complement(5469..5544)
                     /locus_tag="PLD67_02845"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5509..5511),aa:Trp,seq:cca)
     gene            complement(5561..5650)
                     /locus_tag="PLD67_02850"
     tRNA            complement(5561..5650)
                     /locus_tag="PLD67_02850"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5614..5616),aa:Ser,seq:gct)
     gene            complement(5654..5742)
                     /locus_tag="PLD67_02855"
     tRNA            complement(5654..5742)
                     /locus_tag="PLD67_02855"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5705..5707),aa:Ser,seq:tga)
     gene            complement(5749..5821)
                     /locus_tag="PLD67_02860"
     tRNA            complement(5749..5821)
                     /locus_tag="PLD67_02860"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5786..5788),aa:Lys,seq:ttt)
BASE COUNT         2000 a          987 c          836 g         1998 t
ORIGIN      
        1 aatatatttt cccatcttct ttcctactgc cttttctgct tccttatctt ttatttcaag
       61 ttctacaact tcaccatggc tataactgtg agtatttaat gttataccct ctattttttt
      121 tgcaagtgac tcattagctt catacgccaa atccgttctg tacataattc ctcctatcat
      181 agttcctccg ctgccgctca ggatatgcaa aattctatat aaaatttaca tctgattttt
      241 attctgagtt tttttgtatt ttttattctt tttacttgat ttttgcatta atagtctgta
      301 taattgattt gctgtcaaag atgccctagc gataatattg gtgaatgaga ctgcgaaatt
      361 ctgaaatagg aggtggatat tttggcaaat attaaatctg caaagaaaag aatcttaatt
      421 ataaacaaaa aaactcaggc aaataagagc agaaaatctg aaattaaaac atatattaag
      481 aaatttgaaa cagccttaag tgaaggcaac tacgaaaaag ctaaagaata tttaaaaata
      541 tgcgaaaaga aattatatca ggctgctgca aagaatacta ttcataagaa cgctgcagca
      601 agaaaagtaa gtagattaac aaagaaattt aataaagtag cataattata aagacctgat
      661 tggtcttttt aatttgtccc cgtacctatt atcccgtttg atatcaactc aagacctact
      721 ctgctgtcga tttcaccggt ttttatttta ctgtctattt ccaaaaacct cttcatatag
      781 tattcgatag tttccatatt taaatttttg gattgtttag caagcttgga agctacaaaa
      841 ttctttattt tcattttgct cattatttca ttgtaactat atcctttttt ttgtagcatt
      901 acatattgat acagcattct gtactgcctt gtaatcatat gaaatatata ttgctcgggg
      961 gtattgttta aaagcatctc attcaatatc tcaaatgaac ttttcttatc attttcgcat
     1021 atgcagtcaa ctaatctgaa tatattgctt tcaatggatt taaccattaa caaatctaaa
     1081 tcggaagttg ttatattctt tttttcttta ttataagaca taattttatt aatttcatta
     1141 ttgacatgat acaggcttac tgtgctttca tattccaaat aaccggtgtt atttgccata
     1201 taatttgcat cagacattga aattttaaag ttgtttttct tgaattcttc tgtaatatat
     1261 tttgtcagct cgccttccgt cagcctgtta aactcaaata ctgccttatt atccttcagc
     1321 ttctttacaa ttttcttcct tgaatccgct ttcccgtcct taattacaaa aattgatatg
     1381 caagtttcat tctcatcaat gaaatttaat agtctatctt cttctttttt atctaaggat
     1441 cccgtcgatg taaaaaaacc ggcttcctta attacgcata attttttttc tgacataaaa
     1501 ggaaatgtgt aggcaaactc aaaatagtct ttcaagcttg ccggcttttc aaattcggca
     1561 taattcatat cctcatagtt tttatcaata tatttgtatt ttatatactt tatagaataa
     1621 tcaataaaat attcttcgtt cccgtaaagc agcaggcaat tgggaagctt atttttatct
     1681 gccatgtttt taatcaaatt aaagctcata actttcacct ttcaacatcc aaaataataa
     1741 aaaacaaaat atgctcagct tgtatattat tccgattaaa tacaatttat aataattgtc
     1801 ggatttttca ttcatataag tattataata taaattgtta tcttttaaaa ctatatttat
     1861 ttctccatcc tcaaatgtgg aaaaaatctt gctgcctgta tttctgtacc tcgatataac
     1921 ttcttcatga ggaatcccgt aaaaatttct tccatagctt aaaacagcaa ttttcggcct
     1981 tacggcatca acaaactgca ctgttgaaga tgtgttgctt ccgtgatgag gaaccaagac
     2041 tatatcataa gcatttaaac cattataaat agcgtattcc gcttcctctt ctatatctcc
     2101 gggaagcaaa atccttctgc ccttataatt caaagaaatc accaaggatg aattattagt
     2161 attgtttaaa gataaatctt tataaggcca cagtatttcc atacttaaat tatcatcata
     2221 cttcatttga cagcctgcat ttaatttctg cacatttgta taattttcat tgctgtttga
     2281 tgagaatata ttattaacct ttatatggct gtttaaaatg tcatttaagc cggaataatg
     2341 gtcttcgtcc caatggctta taaatattcc atcaacttgt gctattcctc tttttgtcaa
     2401 gtaagggaca gcaatgtatt ctcccatttg cttattagtt gtactgccgc agtcgcaaat
     2461 catatttaat cctttatact tgattgttgt aaataatcct tgtcccacat ctgcaatatt
     2521 taaataaaga ctgtcatcca tcattggaaa tgcaatatag gtaatggaat aaagactgac
     2581 aattgcaaaa ataccgattt ttttgcaatt gcatttttta ttattatgta tgtatatcat
     2641 ataaaataat gtaatataga aaaatatagt ttgacataaa gataaagtag gcattattaa
     2701 gccgttaaaa gcagacttat ctgaaatatc aataatatat ctaaggctgt gcaaaatata
     2761 atcatatata ttaaaaggaa taattaatat aatatgagca aatgaattta atatcaaaag
     2821 aataaatcca taaatcataa taaatgtaaa tacgggcaaa agcagcaagt tataaagaac
     2881 acccatgaca gggacaaaat taaaataata tactattaca ggcaaggtaa atatttgaag
     2941 aaataaatac atatatacgg ttcttaaaac agtggttttt gtctttatat atttgccgta
     3001 tattatgaaa cttagtgctg ccgaaaagga tagtagaaat cctgcatcaa aaatccaaaa
     3061 agggttgtaa atagtcaaca caagagctgc aaggccgatg gaattcagtg aattgtattt
     3121 tctgtacaaa acctcggagc cgaaaagcag agtaaacata accaaagccc tcataattga
     3181 caagggaaac ccgattaaaa acccgtaaaa ccatataata caccaagaca ataaccagct
     3241 gaatcttctg cttaaaacaa atttaaagac atttaataag aaaatataca tgagaacaat
     3301 atgtgttccc gaaacagcaa atatatgggc aagacccatt acctttatat tatcgtaaag
     3361 ctcttcctcc aaatagttta catcacctaa tattattgac aatattattt ccgcattctt
     3421 tttatttaaa ttataataaa atgttttttc ggtatatttt ctgaatttgc ttgatatatt
     3481 gtttaaaaac gaataatttt catcgataac ataagcatct tcgtcggcaa acaccgttgc
     3541 attgattttc ttgctcctca aataattttc ataattaaaa agcattttat ttttgtttaa
     3601 ttgactgccg gcaacattga cccttgcttt tattatgctg ttttcctgaa aatttttata
     3661 tttacctgta tagagaataa tattttcttc atttaaaata ggagtgttgt ttatgcctat
     3721 aactgaagca tagtagcttt cgctgccgga ctttttttgc ttttttacct ttgcttcaac
     3781 tacaatactt tcatttttgt attgagaaag aaccgagctt gaattataat tacagttaat
     3841 gtatgataat atcacaaatg caataattac ggcattgtac atatattttt tatttattaa
     3901 tgaattataa ataagtattg caagaagagc tgtaaaataa tagccgtaca gatttaactc
     3961 ataactgatt attccaaatg catatgctgt aaatattgaa attattatat acattcacaa
     4021 caccctctaa tattttacca aatattagga aaaatttaaa gttattatta aaggaaaact
     4081 atgtttatta atgagtatga agtaaataat ataacggata acccttattt aaatgaaatt
     4141 tataaggaag cagtattcat agatattgaa actaccggtt tgtcgagtat attttcagat
     4201 attatatcga taactttttt gctttataag gatgacaaat atagaatata tcaaatattt
     4261 tgtcaatata agcaagacca gccggatgcc cttaaatatt taaacgagct gataaaattg
     4321 aagaaatata tggtaacttt caacggcaat agctttgata tccctttcct agcagaaaaa
     4381 gcaaaacaat taaacattac tcttgttttt gattcattca ataagattga tttatataat
     4441 tgtataaaaa aatttagata taaaatcaat accgtagatt taaaactaaa aaccgtagaa
     4501 aagtatttct ccattgatag aaatgatact ctggatggca aagatgtgat tacattgtat
     4561 gaagcttaca gattagagca gcgtaaggaa ttttcatatt tgatattaaa gcataattac
     4621 gaggatgtat ataatttgcc atttgttatg aataatattt ttagtttgta tgattttgtt
     4681 atatatacaa aaaaccttat tatcacaatt aataatcagg atattacaat caaaaagaat
     4741 tctattaaat gcaaattcaa tattttatct cttatagcaa aagattatat taatcatagc
     4801 ataaattatg acatggtatt gtcagttaaa cctcaaacat tgaaaattga tattcctctt
     4861 aatatttata aggataataa tataagagag ttttactatt tagacaatga tgaatataat
     4921 attgacaact atacttctat aaagggatta aaaagaaacc tgataccgat taaactaaat
     4981 aataagatgt attatgacaa tataaatcgc attgttaaag aaatcgtaga agaaggattt
     5041 ggttgttccg aaaagcaggt accgtcattc tgacaaccaa aaaagggaac attccttcaa
     5101 caatgagcta tctagaaggt gtgtccccat aacttataaa taaaaaaaga cctaagggtc
     5161 ttttttgatg cccaaacgcg gaatcgaacc acggacacgg agattttcag tctcctgctc
     5221 taccgactga gctatctggg cataatattt agaaataaaa gtgggccttc aaggactcga
     5281 accttggacc taccggttat gagccgggcg ctctaaccaa ctgagctaaa ggcccaaaat
     5341 caattctata atgtggtggg cttggatgga ctcgaaccat cgacctcacg cttatcaggc
     5401 gtgcgctcta accacctgag ctacaagccc aaatcataac atatactaac ccctgccaga
     5461 cctaaaattg gcaggagtgg caggactcga acccacgaca ttcggttttg gagaccgacg
     5521 ttctaccaac tgaactacac tcctagttta tgctatgtaa tggcggagaa ggagggattt
     5581 gaaccctcgc atccttgtca gatctactcc cttagcaggg gagcctcttc ggccacttga
     5641 gtacttctcc acttggcgga gagggtggga ttcgaaccca cgcgggcttt cacccaaacg
     5701 gttttcaaga ccgcctcgtt atgaccactt cgatacctct ccatattatg atccatcggc
     5761 gattcgaacg ccggacacct tgattaaaag tcaagtgctc tgcctgctga gctaatggat
     5821 c
//
LOCUS       DAOSOW010000069         5775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_14049_length_5775_cov_3.740734, whole genome shotgun sequence.
ACCESSION   DAOSOW010000069 DAOSOW010000000
VERSION     DAOSOW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14049_length_5775_cov_3.740734"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="PLD67_02865"
     CDS             complement(<1..562)
                     /locus_tag="PLD67_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016423968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOT21495.1"
                     /translation="MKRTRLKYLIISLTLILLFYIGQAASFLTEGSEKTKSINENTEK
                     AQQIIIGYYPAWKSYSGYTPESVDISKLTHINYAFANIGPDYRIEMGYPDKDPENFEK
                     FRDLKKINPDLKVLISVGGWNWSGRFSDMAATDENRNKFTDSCVEFISKYGLDGVDLD
                     WEYPVGGGLKTNSKRPEDKQNFTLLLK"
     gene            813..2165
                     /locus_tag="PLD67_02870"
     CDS             813..2165
                     /locus_tag="PLD67_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOT21496.1"
                     /translation="MRNNIILTEGKIRSALVKLALPIMGTSFINMAYNLTDIMWLGRL
                     STDAVAAAGAAGYFLWFGSSLVMIAQVGVGVMVAQLFGKGDSEGVKNYINNGLQLNLF
                     IAILYSLFLYIFRHQIIGFFNIENENVSKMAVEYLTIISAGITFHFLNPVFSAILNST
                     GNSITPFKINTVGLIANIVIDPLLIFGFGPLPALGVKGAALATIMAQFFVTMIFLIIG
                     KRYDTIYSHAKLFKKPRLPIIQNIVKIGFPPFTQMGIHAGIAIILTRILAVFGPVAIA
                     VQSIGAQIESITWMTTEGFSAAISAFVGQNYGAGKIKRVKDGYVQGIQIVGSIGIFST
                     LLLIFGAEQLFSLFTPNDPIAIKEGIIYLHILGFSQFLMAIEIGTTGAFNGLGKTIFP
                     TINGIILNVLRIPAALILSRSLGLPGVWWAIAISSNLKGLILFILFYRYSNNFERTYI
                     "
     gene            2444..3367
                     /locus_tag="PLD67_02875"
     CDS             2444..3367
                     /locus_tag="PLD67_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOT21497.1"
                     /translation="MAIRPPILRAGDTVGVVTLGSPRDAESVDSRIQTLESMGFNVVV
                     GEFVYSDAGIVAASPMERAADLMNMFVNPEVRAIIPTRGGTGVSDIIPYLDFDVIRRN
                     PKIITGYSDITILLNVLYQLSDLITFHSLLLIDFRPTTPAYNFDQFFTATSTITAPRP
                     LENPPGIPLISRIEGNVTGTIVGGNLTSFVDTLGTPFEIDTTGRILFIEETHEAASRV
                     YRMIAHLIHAGKFRDCAGVIMGECAGCYANYGKTYEDIINEVIVPLGKPLMTNLASGH
                     GYYKMAIPIGAVTNLNTYNNTLTVLEPTVSL"
     gene            complement(3384..>5775)
                     /gene="ligD"
                     /locus_tag="PLD67_02880"
     CDS             complement(3384..>5775)
                     /gene="ligD"
                     /locus_tag="PLD67_02880"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HOT21498.1"
                     /translation="RNFEKTFEPEGKTQSSEESLRFVVQHHLASRDHYDLRLEWNGVL
                     LSWAVPKGPSYNTKDKRLAVQVEDHPLEYRNFEGTIPKGEYGGGVVMIWDEGYWEPHM
                     NVDEGLSEGMLKFSLKGSRLRGRWALIRLKGKAGETQDNWLLLKEKDEYSKDEEGMSE
                     FTTSIRTGRTMAEIEGGQDEKFIKNPFSKADVQLAKLVKAIPEGDWLYEMKYDGYRIL
                     AFVEGNSVRLITRKGNDITKRFFTIASSLIDLAKGRAMVLDGEIVITDESGKTDFQAL
                     QNYLKTQNSQNLTYIVFDLLAIDGEDIRGQSLILRKEKLESLLQDAPKNLYYSRHVKG
                     YGKESFAAACEAGMEGIVGKKPDSIYSGKRNDDWIKIKCDKRQEFVIGGYTLSDKRSS
                     GVSSLLLGVYEGEDLIYAGRAGTGISENDMKILESKFEGLKCSKPPFKEVPKTKPNEK
                     ITWLEPKLAAEIKFAEWTKDNLLRQASYKGLREDKDTKDIKREKAEGILPQSNDEEGR
                     YMEKDLIIEGVKISSPDKIIYNELDITKEEVIRYYSKVADKMLPYVRHRILSIVRCPK
                     GVSQACFYKKHPGPGSKGIITMPVSTGDDETGEYFYIENATGLISEAQMGTLEFHIWG
                     SSVDNLEMPDMMVFDLDPDEGMDLDKVRQGVIDIRSILDELNLKSYLKISGGKGYHVV
                     IPLKPTADWDKFHDFARQVAEVMENKWPERYTSNVRKAKRKGKIFIDWIRNARGATSI
                     APYSLRARKGAKVSMPIFWEELYTVAPDGINMEDALLRIEKKDPWEDFFQNSQSII"
BASE COUNT         1659 a         1178 c          953 g         1985 t
ORIGIN      
        1 ccttaagaag caaagtgaag ttttgtttgt cttcaggtct ttttgaatta gttttaagac
       61 ctcctccgac cgggtattcc cagtctaaat ccacaccgtc aagtccatac tttgaaataa
      121 actcaacaca gctgtccgta aatttattcc tgttttcgtc tgtggccgcc atgtccgaaa
      181 atctccccga ccaattccat ccgcctactg aaattaatac ttttaaatca gggttaattt
      241 tcttaagatc ccgaaacttt tcaaaatttt cggggtcctt atccggatat cccatttcta
      301 ttctgtaatc cggacctata tttgcaaacg cataatttat atgggttaat ttgcttatat
      361 ctacactttc cggagtatat cccgaataag acttccatgc aggataatac cctataatta
      421 tttgttgagc tttttcagta ttttcattta ttgactttgt tttttcactt ccttcggtta
      481 aaaaagatgc agcttgtcct atgtaaaaca aaagaattaa ggttaatgat atgattaaat
      541 actttaatct tgtcctcttc atatatcctg tatgatgtat agacaatata catcatactc
      601 ctcctataaa acgttttttt aattataaca taataatatt gttataaatc ttgaaatatt
      661 aggcaattcc ttatactctt agcttatttt ccattataag taaccaatcg tgaagtaatt
      721 ttgttttgcc ttattggcaa gtatatgttt tgcttgtaca aagcaaaatt atcagttaaa
      781 ataatacaat aataattact aaggacaaaa gaatgagaaa caatattatt ttgacagaag
      841 gaaaaatacg aagtgcatta gtaaagttag cacttcccat aatgggcact tcatttataa
      901 atatggcata caacttgaca gacattatgt ggcttggcag gctcagtacc gatgccgttg
      961 cagccgccgg tgctgcaggc tattttttat ggtttggctc ttcattggtg atgattgcac
     1021 aggtgggagt tggagttatg gttgcccaat tatttgggaa gggagattcg gaaggtgtaa
     1081 aaaactatat caacaacgga cttcagctaa atcttttcat agctatatta tactctttat
     1141 ttttgtatat tttcagacac caaataattg gtttttttaa tattgaaaat gaaaatgtaa
     1201 gtaaaatggc agttgagtat ttgacaatta tttcagcagg aatcacattt cactttttaa
     1261 atcctgtttt ctcagcaatt ttaaacagca ccggaaacag tattactcct tttaaaatta
     1321 atacagtggg tcttattgca aatattgtta ttgacccttt gttgatattc ggtttcggtc
     1381 ccttgccggc attaggtgtt aaaggtgctg ccttagctac aataatggct caattcttcg
     1441 tgacgatgat tttcttaatt atagggaaaa gatatgatac tatttattca catgcaaaat
     1501 tatttaagaa accaagacta cccattattc aaaatatagt caaaatagga tttcctccct
     1561 ttactcagat gggtatccat gcgggcatag caataattct aaccagaata ctggcagttt
     1621 tcggacccgt tgcaattgcc gttcaaagta taggcgctca aatcgagtcc attacatgga
     1681 tgacaactga aggtttttct gcagctatat ctgcatttgt agggcaaaat tacggtgccg
     1741 gaaaaataaa acgagtcaaa gacggatatg tacaggggat tcaaattgta gggagtatcg
     1801 gaatattttc aaccttgcta ttgatttttg gtgctgaaca actattttca ttatttactc
     1861 ccaatgaccc gattgcaata aaggaaggaa taatttattt gcatatatta ggtttttctc
     1921 aattcctcat ggcaattgaa attggtacta ccggtgcatt taacggatta ggaaaaacaa
     1981 ttttccccac gataaacgga attatactga atgtacttag aataccggcc gcattgatac
     2041 taagcaggtc attaggattg ccgggtgttt ggtgggcaat agctatatct tcaaatttaa
     2101 agggattaat attatttatt ttattttata gatactcgaa taatttcgag cgaacctata
     2161 tttagtattt ttttcatctg agtgttcaaa atttcaatta atgtgtgaat taaaaaatta
     2221 atttattcca ttttagacac attaattaga atttcgagca tatatgacct gtagaagtat
     2281 cttttatatc aagataacca ttaattgaat ttttgagcta ttcaatttac aatttcatat
     2341 atgctttgat tttttcttct gccgatgaac ttcttattta ttaaccaaca tagctttttc
     2401 tcatataata actaaataga atggatatta ttaggaggaa agaatggcta tcagaccacc
     2461 tattttaaga gcaggagata ccgtaggcgt agttaccttg ggtagtcctc gtgatgccga
     2521 atctgttgac tctagaattc aaacattaga atctatgggt tttaatgttg tagtaggcga
     2581 atttgtatat tccgatgcag gtattgttgc tgcttcgccc atggaacgtg ctgcggattt
     2641 aatgaatatg tttgtgaatc ccgaagttag agctattata cctacaagag gtggtacggg
     2701 agtttcggat atcattcctt atcttgattt tgatgtaata aggcgcaacc ctaagattat
     2761 aaccggatac agcgatataa caattttatt gaatgtttta taccaattgt cagacttaat
     2821 tacctttcac agcttattgt taatagattt tagacccact acccctgcat ataattttga
     2881 ccaatttttt actgccacat caacaataac tgcaccaaga cctttggaga atccccccgg
     2941 tattccgctg ataagcagaa ttgagggaaa tgtaacaggt accatagtag gaggcaatct
     3001 aacgtctttt gttgatacac ttggaacacc ctttgaaatt gatactaccg gaaggatttt
     3061 atttattgaa gaaactcacg aagcagcaag ccgtgtttac agaatgattg ctcatttgat
     3121 tcatgcaggt aaattcagag attgcgccgg agttataatg ggagaatgtg caggttgtta
     3181 tgcaaattac ggaaaaacct atgaagacat aataaatgaa gtaattgttc ccttaggcaa
     3241 acctcttatg accaatttag cttcagggca cggctattat aaaatggcca tacccatagg
     3301 ggcagttact aatcttaata cttataataa tacattgaca gtattggaac ctacagtgag
     3361 cctgtaggtt cttgcaggtt attctatata atcgattggc tgttttggaa gaaatcctcc
     3421 caagggtcct ttttttcaat ccttaaaaga gcatcctcca tattgatgcc atccggcgca
     3481 accgtataaa gctcttccca aaatataggc atagacacct tagctccttt tcttgccctc
     3541 agtgaataag gggcaatact tgtagctccc cttgcattcc gaatccagtc aataaatatt
     3601 ttgccttttc ttttagcttt tcttacattg cttgtatatc tttccggcca cttattttcc
     3661 attacctctg caacctgtct tgcaaaatca tgaaatttat cccaatcggc ggtaggtttt
     3721 aacggaatta ccacatgata tcccttacca ccgcttatct taagatatga ttttaaattt
     3781 agttcatcaa gtatacttct aatgtcaata accccttggc gaactttgtc taaatccatt
     3841 ccttcatccg ggtccaaatc aaataccatc atgtccggca tttcaagatt atcaacactg
     3901 cttccccaaa tatgaaattc caaagtaccc atctgggctt cagatattaa ccctgttgca
     3961 ttctctatat aaaagtattc acctgtttca tcatcgcctg ttgaaacggg cattgtaata
     4021 attcctttgc ttccgggacc ggggtgcttt ttataaaagc acgcttggga tactcccttg
     4081 gggcaacgta caatacttaa aattctatgc ctcacataag gaagcatttt atctgcaacc
     4141 tttgaatagt aacgaataac ttcttccttt gttatatcga gttcattgta tatgattttg
     4201 tcaggacttg aaattttaac tccttcaata ataagatctt tttccatgta tcttccctct
     4261 tcatcatttg attggggcag aatcccctct gccttttccc ttttaatatc cttagtgtcc
     4321 ttatcttccc ttaatccttt gtagcttgct tgccttagca gattatcctt ggtccattct
     4381 gcaaacttta tttctgcagc caatttgggc tcaagccagg taatcttttc attaggcttt
     4441 gtctttggca cctccttgaa aggaggcttc gagcacttta aaccctcaaa tttactctca
     4501 agaattttca tatcattttc actgattccc gtgcctgcac gtcctgcata tattaaatcc
     4561 tcaccctcgt aaactccgag aagaagggag cttactccgc tgcttctttt atctgaaagt
     4621 gtatatcctc cgataacgaa ctcttgtctt ttatcgcatt taatttttat ccagtcatca
     4681 ttcctttttc cgctgtaaat ggaatcaggt tttttcccca ctattccttc cattccagct
     4741 tcacaagctg cagcaaagct ttcctttccg tatcctttca catggcggct gtagtaaaga
     4801 ttttttggcg catcctgcaa taaactttca agcttttctt ttcttagaat taaagattgt
     4861 cctcgaatat cctctccgtc aatggcgagc aaatcaaaga caatataagt aagattttga
     4921 gagttctgcg tttttaaata gttttgtaaa gcttgaaaat ccgtcttgcc cgattcatcc
     4981 gtaataacta tttcaccgtc taataccatt gctcttccct tagccaagtc tataagcgaa
     5041 gaggctatcg taaaaaaccg tttggtaata tcattgccct ttcttgttat aagacggaca
     5101 ctgttgcctt ccacaaatgc caaaattcta tagccgtcat atttcatttc ataaagccaa
     5161 tcaccttccg ggattgcctt aactaatttg gcaagctgaa catcagcttt actgaaagga
     5221 tttttgatga acttttcatc ttgacctcct tcaatttcag ccattgttcg tccggtcctg
     5281 atactggtgg taaattcgga cattccttct tcatccttag aatactcatc cttttctttt
     5341 aataaaagcc agttatcctg tgtttcacct gccttacctt ttaaccttat caaagcccac
     5401 ctgcctctaa gccggcttcc tttcagactg aatttgagca taccttcact gagaccttca
     5461 tccacattca tatgaggctc ccagtatcct tcatcccaaa tcattacaac cccgccgcca
     5521 tattccccct taggaatggt accttcaaaa ttcctgtatt ccaaagggtg gtcctctacc
     5581 tgtacggcaa gccttttatc cttagtattg taagaaggcc ccttgggtac tgcccagctt
     5641 agaagaacac cattccattc caaacgcaaa tcatagtggt ctctgctggc taaatgatgc
     5701 tggacaacaa atcttaaact ttcttctgag ctttgtgtct ttccttccgg ctcaaaagtt
     5761 ttttcaaaat ttctt
//
LOCUS       DAOSOW010000070         5744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_14150_length_5744_cov_4.781508, whole genome shotgun sequence.
ACCESSION   DAOSOW010000070 DAOSOW010000000
VERSION     DAOSOW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14150_length_5744_cov_4.781508"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..1742
                     /gene="nhaC"
                     /locus_tag="PLD67_02885"
     CDS             300..1742
                     /gene="nhaC"
                     /locus_tag="PLD67_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005902038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HOT21499.1"
                     /translation="MENDTKKPSLGVALLIVAFMFVVIIAQLIAAGSPDIHLTMVFSI
                     TFATILLMFGGTKWSVIEEGIMHGCKIAMIPMLILMFIGMLIPTLIAAGTIPALIYYG
                     LKIINPSAFLLTAAMVCSVASLATGSSYTTGGTFGVAFMGIGIGLGIPPAMTAGAVIS
                     GAVIGDKLSPISDSTNLAAGVTETNLFKHVKSMMYTTIPAFIISCTLYGFLGMNYSAA
                     SIDTTTVDVILNGISSVFNVSFGSTLISMIPLAVVLFMAVKQYSALATMVAASLVAMV
                     IAMITQGFSIIEMMSFMNYGFSIDVGVEAVNKLLNKGGIQSMMWTVSLGFLGLSYGGI
                     LEKSGVMETLLGSMKNLTKNAGNLITTHVFSTIIVNMLSASQYVSIIIPGRMYLPAYR
                     KLGIRSDVASRTCEDAGTVTSPLVPWGLCGVFFTGVLGVNTLEYLPYSFLAYITPVIA
                     IIYAYTGKFIWYEKDNMIKSNVEKASEING"
     gene            1772..2962
                     /locus_tag="PLD67_02890"
     CDS             1772..2962
                     /locus_tag="PLD67_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005947502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT21500.1"
                     /translation="MYEIEKGQLTELLTNLVSIESPYLEEDEIMEYVYSWFRKNDIKA
                     FVHHYNEAKVTGFKGKNIVANIEGNKNGPVICLNGHLDTVKLCNGWTHNPKGEIKDDR
                     LYGVGALDMKSGCAATMIAIKAFAENHKDFRGEIIATYVSVEEGPYGMGTNALIDEGY
                     LKDVDFSISTEPSAGFSGNKFPDVCLGARGGYGLDIEFFGVSSHAATPELGVSAAEDA
                     STVISELKKIKYIKDPHLGIGNACVIGIECDGGACSVPDYAKVKLFWHIVPGENEETI
                     TGEIEKAIRRANIKCKHKINFRESPSEGSKGFMPYTVSEEDPIVKKFLETVEEVCKAK
                     PVISYFQSIGDFNYLGSRLGAPVVIFGASGKNFHGKDEYVELDSVVKTAETVYRFLER
                     MLCL"
     gene            complement(2996..4195)
                     /locus_tag="PLD67_02895"
     CDS             complement(2996..4195)
                     /locus_tag="PLD67_02895"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HOT21501.1"
                     /translation="MLSINFLGKPRVEYDGKKLEEQLGSKAVVLICLLVLNEKRYMSR
                     EKLEGYLWPDSSTDAARYNLRYNLWLIKKNIGPDHKGHQFLHVDSDCCSINKEYEFEC
                     DTFEIMSFKHSQNDSLESILKLKQLFRGDLLEGCFFKNCDELNDLIIFERMKYEQRKV
                     KILKSLVDYYEKEESFDYCLEVINEILEIEPYDEEMILKILDIYVKCGKPVAAITYYN
                     NFSKLLTSSLGVSPSNRLRKKYTEIRSALSDSFGINNLKVNMDTFIKGSGNQEYVSYT
                     KIISDCIKNIEYFWIADVVDKIIAALGPDYMNQLSEKELLDLAYVQSDILNYYGDKFE
                     LVKDYGRDTMSVCIINAFIKLLRIICSKQELTIIIQNSIDMDDISLNVLKYLKRLKIE
                     GLKLIEE"
     gene            complement(4215..>5744)
                     /locus_tag="PLD67_02900"
     CDS             complement(4215..>5744)
                     /locus_tag="PLD67_02900"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011016557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOT21502.1"
                     /translation="HASILIKNGRCMTMEGKEEKKWIAVEQNKIVAMGDNTEYESLID
                     GDTIVFDAGGNTVLPGFIDSHFHVIQTALDSISVDLSCAKTYEELGEQLREAASSFTG
                     GYIRGIRISEEQFTEGRFPGRTLLDKYCSDVPVVLNSMEYQVSILNTYAMLYFKIPFT
                     AEGIELDRKQMPTGIFRKQANAILRTNILKNISDNIRKDAVEGIMGTLLMNGITTINA
                     MEGGFMYSDKDADFLYEHGKEFPIDIELFYQTMDIEKIKAMGLKRLGGCLYVDGTFGA
                     RTAALSFQYADCPGTMGSLLLTQQEINDFVLNCYKNNLQLALYTIGDRAIEMAINAHE
                     NAFYQTGIIGLRHRLEHAELINEKQIQRAKELGILLSMQPSYETYWGGPGKMYEQRLG
                     DKYILTNPFKEIIEKGVIICGGSDSDVTEPRPLLGIHSAVNHPVVKHRVDIYEALKMF
                     TCNGAYGIFQEKNKGTLSVGKTADILILDRDILLVPSDQIEKARVRVTIKSGETLYSN
                     L"
BASE COUNT         1743 a         1035 c         1072 g         1894 t
ORIGIN      
        1 cgggattcat atggagatta ataaggaatt aattttggaa atctaagagc gaagcgtagc
       61 tgctgcagca aagggactgt ttcatattgt gatttgatac agtcccttct atattcccat
      121 ttattactcc ggagccattt gccggagttt actccgatat tatactgtta aaaaatgttt
      181 ataaagtgtt taattgtaat ctggttcaaa aaaactaaat aataattaaa catataaata
      241 taaataaaaa caacacctct gatatataat tgatataaaa ttagtgagga ggagatattt
      301 tggaaaatga tactaaaaaa ccatcattag gtgtagcact gttaattgtc gcttttatgt
      361 ttgttgttat cattgctcag ctcatagctg cagggagtcc tgatattcac ttgacaatgg
      421 tattctcaat tacatttgcg acaatattgc tgatgttcgg cggaacaaag tggtcagtaa
      481 ttgaagaagg tataatgcat ggatgtaaaa tagcaatgat tccgatgctt atactgatgt
      541 ttatcggtat gcttatccca acacttattg cagcaggaac cataccggca cttatttatt
      601 atggtttgaa aatcatcaac ccttcagctt tcttgcttac tgctgcaatg gtatgcagtg
      661 tagcatcatt ggctacagga agcagttata ctacaggagg tacttttggg gttgccttca
      721 tgggtatagg aataggtctt ggaataccgc cggcaatgac agcaggtgca gttatttcag
      781 gcgctgtaat cggagataaa ttatcaccta tatcagattc gacgaattta gcagcaggtg
      841 ttactgaaac gaacttgttt aagcatgtca aaagcatgat gtacacaact attcccgcat
      901 ttataatttc atgcactctt tatgggtttc taggaatgaa ttacagtgct gcctcaatag
      961 atacgacaac agttgatgtt atattgaacg gcatttcatc agtatttaac gtatctttcg
     1021 gctcaacgct tatcagcatg attccgttag ctgtagtttt gtttatggct gtcaaacagt
     1081 atagtgctct tgccacaatg gtggcagcgt ccttagtagc tatggtaata gcaatgatta
     1141 ctcagggttt tagtattatt gaaatgatgt catttatgaa ttatggattt agtattgatg
     1201 tgggagttga agcagttaat aagcttctta acaagggcgg tatacagtct atgatgtgga
     1261 cagtatcatt aggtttctta ggcctttctt acggcggtat tcttgaaaaa tccggtgtta
     1321 tggaaacact gttaggcagc atgaagaacc ttacaaaaaa tgcaggaaat cttattacca
     1381 cacatgtgtt ttctacaata atcgtaaata tgttatctgc aagtcaatat gtttcaatta
     1441 taatacccgg aagaatgtac ctgcctgcat ataggaaatt aggtataaga agcgatgttg
     1501 cttcaagaac ctgtgaagat gcgggtacag taacatcacc tttggtacct tggggattgt
     1561 gcggtgtatt ctttacggga gtacttggcg taaacacctt agaatatctt ccatattcat
     1621 tcttggcata tataactccc gttattgcaa ttatttatgc atataccggt aaatttattt
     1681 ggtatgaaaa ggataatatg ataaagagta acgtggaaaa ggcttcagaa ataaacggat
     1741 aaagaaaatc ggctttagaa agggaataaa gatgtatgaa atcgaaaaag gccaattaac
     1801 agaattatta acaaatttgg tttctattga aagcccgtat ttagaagaag atgagataat
     1861 ggagtatgta tacagttggt tccggaagaa tgatataaag gcctttgttc atcattataa
     1921 tgaagcaaaa gttacaggct ttaagggaaa gaatattgtt gcaaatattg aaggtaataa
     1981 gaacggaccg gtaatttgtc tgaatggtca tttagatacc gttaagttgt gtaacggatg
     2041 gactcataac cccaaaggag aaataaaaga tgataggctc tatggagtag gagctttaga
     2101 tatgaaatcc ggatgcgcag caacaatgat tgcaataaaa gcttttgctg aaaatcacaa
     2161 ggattttaga ggtgaaataa ttgccacata tgtttcagta gaagagggac cctatgggat
     2221 gggaacaaac gctttgattg atgaaggata tttaaaggat gttgattttt caatcagcac
     2281 ggaaccaagt gctggatttt caggcaacaa atttcccgat gtttgtttag gtgctagagg
     2341 tggatacggt ttggatatag aattcttcgg tgtatcttct catgcagcga ctcctgaact
     2401 gggagtaagt gcggcggagg atgcttcaac ggtaatcagc gaattgaaaa aaattaaata
     2461 tattaaagac cctcatcttg gaataggtaa tgcttgtgtt attggtatcg agtgtgacgg
     2521 aggtgcatgc agtgttcctg attatgctaa agtgaagcta ttctggcaca ttgttccggg
     2581 agaaaatgaa gagacgataa caggagagat tgaaaaagca ataaggagag ctaatattaa
     2641 atgcaaacac aaaattaatt ttagagagtc accttccgaa gggtctaaag gttttatgcc
     2701 ttacacagtt tctgaagaag accccatagt taaaaaattt ttggaaactg ttgaagaggt
     2761 atgtaaagcc aagcccgtga tttcttactt tcaaagtatc ggagatttta attatttagg
     2821 ttcaagactg ggtgctccgg ttgttatctt cggtgcttca ggcaaaaact ttcacgggaa
     2881 ggatgaatat gttgaactgg actcagttgt aaaaactgct gaaactgtct acagatttct
     2941 tgaaaggatg ctgtgtttat agggcaataa ttgataatgc gcacgttagt gcgcattatt
     3001 cttctattag ctttagccct tctattttta atcgtttaag atattttagt acattaagag
     3061 agatatcatc catatcaata ctgttttgaa taatgatcgt aagctcctgt ttactgcaga
     3121 taatccttaa taattttata aaagcattta ttatacatac actcattgtg tcccgcccat
     3181 aatcctttac aagttcaaac ttatcgccgt aataattcaa aatgtcactt tgtacatatg
     3241 ccaagtccag caattctttt tcgcttaatt gattcatgta atcaggacct aaagctgcaa
     3301 ttattttatc aactacatcg gcaatccaaa aatactctat gtttttaata caatcggaaa
     3361 taatctttgt atacgagaca tactcttgat taccagagcc ttttatgaat gtatccatat
     3421 ttactttcaa attattaata ccaaaagaat ccgataatgc ggatcttatt tctgtatact
     3481 ttttcctaag ccgatttgaa ggagataccc caagactgct tgtcagcagt ttgctgaaat
     3541 tgttgtaata tgttattgcg gcaaccggtt ttccgcactt tacataaata tcaagtattt
     3601 ttaggatcat ttcctcatca tagggttcga tttcaaggat ttcatttatt acttcaaggc
     3661 agtaatcaaa gctctcctct ttttcatagt agtccaccaa actcttcaat attttaactt
     3721 ttcgctgttc gtatttcatt ctttcaaata tgataagatc gttgagttca tcacaattct
     3781 taaaaaaaca gccttccagc aaatctcccc tgaataattg tttgagcttt agaatactct
     3841 ccaaagagtc attttggctg tgtttaaagc tcatgatttc aaaggtatca cattcaaatt
     3901 catattcttt atttatggaa cagcaatcac tatctacatg caaaaattga tgccccttat
     3961 gatcaggtcc tatatttttt tttataagcc acaaattata tcttaaatta taccttgctg
     4021 cgtccgtact gctgtcaggc cagagataac cttcaagctt ctctctgctc atatatcttt
     4081 tttcatttaa cacgagaagg cagattaaaa caacagcctt actgcccaac tgttcttcca
     4141 gttttttacc gtcatattca actcttggct tacccaaaaa attaatactc agcatactgt
     4201 ttcaccgatc cctatcaaag gttactatat aatgtttcac ctgattttat ggttacgcga
     4261 acccttgctt tttctatttg atctgacgga actaaaagta tatctctatc caatattaaa
     4321 atatctgctg ttttccctac acttaaagtg cccttgtttt tctcttgaaa aatgccgtat
     4381 gcgccattgc atgtaaacat tttcaatgct tcataaatat caactctatg ctttacaacc
     4441 gggtgattta ctgccgagtg aatacccagt aagggccgtg gctccgtaac atcactatcg
     4501 gagcctccgc atattattac acctttttca ataatttctt taaatgggtt tgtaagaata
     4561 tatttgtctc ccagcctttg ttcatacatc tttcctggac ctccccaata ggtttcatag
     4621 gaaggttgca tagaaagaag gattcctaat tctttagctc tttgtatttg tttttcattt
     4681 atcaattcag cgtgttccaa acgatggcga agtccaataa ttccggtctg ataaaatgcg
     4741 ttttcatggg catttatagc catttctata gctctgtcac cgattgtgta taaagcaagc
     4801 tgaaggttgt ttttataaca gttcaaaaca aaatcgttga tttcctgctg agttagaagg
     4861 aggcttccca tggtacccgg acagtcagcg tattgaaaag aaagtgctgc ggttctggcg
     4921 ccaaaagtgc catcgacata aaggcatcct ccaagtcgct ttaatcccat ggctttaatt
     4981 ttttcaatgt ccatagtttg gtaaaatagt tcaatatcta taggaaactc tttgccatgc
     5041 tcatatagga aatctgcatc tttatcactg tacataaatc cgccttccat ggcgttaatg
     5101 gtagttatgc cattcatgag aagagttccc attattccct caactgcatc tttcctgatg
     5161 ttatctgaaa tattttttag tatatttgta cgcaaaatag cattggcttg tttcctaaaa
     5221 atacctgtgg gcatttgttt tctatccagt tcaatacctt cagctgtaaa aggtatttta
     5281 aaatatagca tagcataggt gtttaatatg cttacctgat attccatgga attaagtaca
     5341 acaggaacat cactgcaata cttgtcaagg agcgttctcc cggggaatct cccctctgta
     5401 aactgttctt ccgatatacg gattcccctg atatagcctc cggtaaaaga ggatgctgct
     5461 tctcttagct gttcccccaa ctcttcatag gtttttgcac agcttaaatc tacacttata
     5521 gagtcaagtg cagtttgaat cacatgaaaa tgactgtcga taaatccggg aagcacagta
     5581 tttccccctg cgtcaaacac aatcgtatcc ccgtcaataa gagactcata ttctgtgtta
     5641 tcccccatag caacgatttt gttttgttca acagcaatcc atttcttttc ttcttttcct
     5701 tccatagtca tacatcttcc gtttttaatc agaatacttg catg
//
LOCUS       DAOSOW010000071         5623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_14551_length_5623_cov_3.906968, whole genome shotgun sequence.
ACCESSION   DAOSOW010000071 DAOSOW010000000
VERSION     DAOSOW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14551_length_5623_cov_3.906968"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1565)
                     /gene="glnA"
                     /locus_tag="PLD67_02905"
     CDS             complement(132..1565)
                     /gene="glnA"
                     /locus_tag="PLD67_02905"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOT21503.1"
                     /translation="MFSTVNEVQEYCVSNKIKMIDFKMIDINGRWRHLTIPAERLNED
                     TMKYGIGFDGSNYGYATVEKSDMIFIPDLTSAHIDPFVDIPTLSMIGNVFVLHPDGNK
                     PFNQYPRNVALRAEEYLRESKIADSMIIGPEFEFHVLDHISYECKPNKVAFEMDTSQA
                     EWNSGNNESRNLGYTVPLKSGYHITAPHDITYGLRSRMCLLLEDWGVEVKYHHHEVGG
                     PGQVEIEVELGELTEMADKTMIIKYVVKNSAIAENKTATFMPKPFFAEAGNGMHVHML
                     LMKDGKPVFYDENGYSGLSREAHWFIGGLLKHVRSLCGLTNPSTNSYRRLVPGYEAPV
                     TIGYATSNRSAVVRIPAYCKSPEAKRFEIRNPDGTCNPYYAYAAILMAGIDGIINKID
                     PHENGWGPYDFNLYNLSPEEQKKLSALPQSLDEALKALEEDHDYLLRGGVFPEELLNT
                     WIANKRMEMNEINKIPHPAEFAYYYDL"
     gene            1806..4451
                     /gene="ppdK"
                     /locus_tag="PLD67_02910"
     CDS             1806..4451
                     /gene="ppdK"
                     /locus_tag="PLD67_02910"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOT21504.1"
                     /translation="MSKKWVYLFKEGSASQKSLLGGKGSNLAEMTNIGMPVPQGFIVT
                     TEACTEFYNQGEKLSEEIISQIRTNLSILEKQTEKVFGDTNNPLLVSVRSGAKFSMPG
                     MMDTILNLGLNEDTVEGIARKTGNTRFAYDSYRRFIQMFSDVVLGVPKSLFENALEAK
                     KEKNGYKFDTELTADDLKELVQEFKQIYKNEVGEDFPDNPEKQLLMAVEAVFKSWNNQ
                     RAITYRNLYNIPHDIGTAVNVQSMVYGNMGDTSGTGVAFTRNPSTGEDKVFGEFLINA
                     QGEDVVAGIRTPLTIDKLKDIMPEIYDEFINICEKLEQHYKDMQDIEFTIEEGKLYIL
                     QTRSGKRTADAALRAAVEMVDEGLISEEEAVLRVEPKSLDQLLHPKFDPVELKKSSPI
                     AKGLPASPGAASGKIYFTAEDAVEASLKGEDAILVRKETSPEDIEGMSKAKGILTSRG
                     GMTSHAAVVARGMGKCCVAGCESVSVDEKNKTMTVNKAVYHEGDYISLDGSTGSVYAG
                     IIKTVPANISGNFEKLMKWADEFRRLKVRTNADTPHDSEAAVKFGAQGIGLCRTEHMF
                     FEESRIFSVRKMIISDTVEQREKALAEIMPMQKNDFKGIFKAMGVRPVTIRLLDPPLH
                     EFLPTEEDDLIELAEAMNVSLQEVKAKVNSLHEFNPMLGHRGCRLAISYPEIAKMQTR
                     AIIEAAIEVSREENLEIIPEIMVPLVGKKEELLILRKLIIETAEETKKEFNSDLNYLV
                     GTMIEIPRACLTADEVAEYADFFSFGTNDLTQMTFGYSRDDAGKFLEDYKEARILEQD
                     PFETIDQEGVGILVDMAVKLGRKAKEHLHLGVCGEHGGDPASVEFFHRVGLDYVSCSP
                     FRVPVARLAAAQAALKDKMNVHIDK"
     gene            4525..4875
                     /locus_tag="PLD67_02915"
     CDS             4525..4875
                     /locus_tag="PLD67_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019258623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HOT21505.1"
                     /translation="MTNPSIYKMSVAKVYPFYVAKAEKKGRTKAEVDEIIRWLTGYSQ
                     EEFQEQLNKQTDFETFFLEAPLPNPARTLIKGVVCGVRVENVEEPIMREIRYLDKLID
                     ELAKGKAMDKILRK"
     gene            5044..5571
                     /locus_tag="PLD67_02920"
     CDS             5044..5571
                     /locus_tag="PLD67_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT21506.1"
                     /translation="MDEKIIELVKSSKDKGLTLMQEQYSGLMHYIVRGILQNESDIEE
                     CISDICIKVWNSIESYSPEKGKFSTWLTVISRNTALNYMKSKYTEHDELKEETADKHS
                     VEDDVLRRERAEELKCAIRTLSPEEQHIFYRKYYYLQRTSQIAAELGMTERSVEGRLY
                     RLRKKLQKKLGGDFI"
BASE COUNT         1929 a          996 c         1133 g         1565 t
ORIGIN      
        1 atatactaag acaatactga tttctgtata caaaaaattt acctcctgat agattttgta
       61 aaataattaa ttacattatc tactttggag gtaataatgt gcattcggaa aggcgctaat
      121 tcatagaaca actacaaatc ataataataa gcaaattcag cagggtgagg tattttattg
      181 atttcgttca tttccattct tttatttgca atccatgtat tcagtaattc ttcagggaat
      241 actcccccgc gaagcaaata atcatggtct tcttccagag ctttaagagc ttcgtccaat
      301 gattgaggaa gggctgaaag ttttttctgc tcctcaggtg aaagattgta tagattaaaa
      361 tcatatggac cccagccgtt ttcatgaggg tctattttat tgattatacc gtctatacct
      421 gccataagta tagctgcata agcataataa ggattgcatg ttccatccgg atttcttatt
      481 tcaaaacgtt ttgcttcagg cgatttgcaa tacgcaggta ttcgtacaac tgcacttctg
      541 tttgaagttg cataacctat cgtaaccgga gcctcatatc ccggcacaag gcgtctgtat
      601 gaatttgtgg aaggatttgt caaaccgcaa agggacctga catgtttcaa taaacctcct
      661 ataaaccaat gagcctcccg gctgagtccg gaatatccgt tctcatcata gaaaacaggt
      721 tttccatcct tcatcaagag catatgtacg tgcatgccgt tcccggcttc cgcaaaaaac
      781 ggtttaggca taaaagttgc agttttgttt tctgctatgg cggaattctt aacaacatat
      841 tttattatca tcgttttgtc ggccatttca gttaattctc caagctcaac ctcaatttca
      901 acctgtccgg ggccgcctac ttcatggtgg tgatatttaa cttctacgcc ccaatcctcc
      961 aaaagaagac acatcctgct ccttagtccg taagttatat catgaggcgc agttatatga
     1021 taaccgcttt ttaaaggtac ggtataacct aaatttctgc tttcattatt accgctgttc
     1081 cactcagctt gtgatgtatc catctcaaaa gctaccttgt ttggtttaca ttcataacta
     1141 atatgatcca gaacatgaaa ttcaaattcg ggaccaataa tcatggaatc agcaatttta
     1201 ctttctcgta aatattcttc ggctcttaat gcaacattcc tggggtattg attaaagggt
     1261 ttattcccgt caggatgcaa aacaaataca tttccaatca tagaaagtgt aggtatatca
     1321 acaaaaggat cgatatgtgc gctggttaga tcaggaataa atatcatatc gcttttctca
     1381 acggtagcat aaccgtaatt tgacccgtca aaaccgatac catacttcat tgtatcttcg
     1441 ttaagtcttt cggcgggaat agttaaatga cgccagcgtc cgtttatgtc aatcatttta
     1501 aagtcaatca ttttaatttt gttactgaca caatactctt gcacttcatt tactgttgaa
     1561 aacataaaaa cctccaaaaa tttttttagg tagcttatgg taatattata caacatataa
     1621 aaattaaaac aagccttatt taaccttgcc gttatatgtc agtccggtat attattttat
     1681 attttttgaa ataatactgt actttttatt aaatttataa tattattggt ctatataatt
     1741 tattttagga ataatactca attaatgagt ttgcaataat aataggaata tagaaggagg
     1801 ttcatatgag taaaaaatgg gtttacttat tcaaagaagg aagtgcttct caaaaaagct
     1861 tattgggagg aaagggctca aatttagcag aaatgactaa tataggaatg cccgtacctc
     1921 aaggatttat agttacaacg gaggcttgca cggaatttta caatcaagga gaaaaattat
     1981 ctgaggaaat tatcagccaa atcaggacta atttatctat tctggaaaaa cagacagaaa
     2041 aagtatttgg tgatactaat aaccctcttt tagtatctgt ccgctcggga gctaaatttt
     2101 caatgccggg aatgatggac acaattttaa accttggttt aaatgaagat actgttgaag
     2161 gtattgcaag aaaaacagga aatacacgct ttgcatacga cagttacaga agattcattc
     2221 aaatgttcag cgatgtagta ttaggagttc ctaaatcttt gtttgaaaat gctctggaag
     2281 ctaaaaaaga aaaaaacggt tacaaatttg atactgaatt gactgcagat gatttaaaag
     2341 aacttgtaca ggaattcaaa caaatttaca agaatgaagt aggtgaagat tttcccgata
     2401 atccggaaaa acaactttta atggcagtag aagcagtatt taaatcatgg aataaccaaa
     2461 gagctattac atacaggaat ttatacaata tacctcacga tataggaaca gcagtaaatg
     2521 ttcagtcaat ggtatacggc aacatgggag acacatcagg aacgggagtt gcattcacaa
     2581 gaaacccgtc caccggagag gacaaagtat tcggtgaatt cctgataaat gcccaaggcg
     2641 aagatgttgt tgccggaata cgtacacctc taacaattga taagctaaag gatattatgc
     2701 ctgaaatata tgatgaattc ataaatattt gcgaaaaact cgagcagcat tacaaagaca
     2761 tgcaggatat agagtttaca atagaggaag gcaaactcta catacttcaa acacgttcag
     2821 gcaaaagaac agctgatgca gcattaagag ctgcggttga aatggttgat gaaggattga
     2881 tatctgaaga agaagcagtt ttaagagttg aaccgaaatc attagaccag cttttgcatc
     2941 ctaagtttga ccctgtagag cttaaaaaat catcacctat tgcaaaaggg ctgccggctt
     3001 caccgggagc agcctccggg aaaatatact ttacagccga agatgcggtt gaagcttcat
     3061 taaaaggtga agatgcaatt cttgtaagaa aagaaacatc tcccgaagat attgaaggaa
     3121 tgagcaaagc taaaggcata ctgacttcaa ggggaggaat gacctctcat gctgcagttg
     3181 ttgcaagagg tatgggtaaa tgctgtgttg ccggctgtga atcggtatca gtagatgaaa
     3241 agaataaaac aatgactgta aacaaagcag tttaccatga aggcgattat atttcactcg
     3301 acggctcaac aggaagcgta tatgcgggaa taataaaaac agtacctgcc aacatatcag
     3361 gaaattttga aaaattaatg aaatgggcag atgaattcag aagacttaaa gttcgaacaa
     3421 atgcggatac accccatgat tcagaagcag ccgttaaatt cggagcacaa ggtataggac
     3481 tttgcaggac tgagcacatg ttcttcgagg aaagccgtat attctccgtt agaaaaatga
     3541 ttatttctga tacagttgag caaagagaaa aagcattggc agaaattatg cctatgcaga
     3601 aaaacgactt taaaggaata tttaaagcaa tgggcgttcg tcctgttaca atcaggcttc
     3661 ttgaccctcc gcttcatgag tttttaccta cagaggaaga tgatttaata gaattggctg
     3721 aagctatgaa tgtttcttta caggaagtaa aagccaaagt caacagtctc cacgaattca
     3781 acccgatgct tggtcacagg ggctgccgcc tggcaatttc atatcctgaa atagcaaaaa
     3841 tgcagacaag agcaataatt gaagctgcaa ttgaagtttc aagggaagaa aatttagaaa
     3901 ttataccgga aataatggtt ccgcttgtag gcaaaaagga agaactattg atactaagaa
     3961 aattaataat cgaaacagct gaagaaacta aaaaggaatt caattccgat ttgaactatc
     4021 ttgtaggcac aatgattgaa ataccgcgtg catgcttaac agctgacgaa gtagcagaat
     4081 atgccgattt cttctccttt ggaacaaatg acctcacaca gatgacattt ggttattcaa
     4141 gggatgatgc aggcaaattc ctagaggact acaaggaagc aagaatcctc gagcaagacc
     4201 cctttgaaac tatcgaccag gaaggtgtag gcatactagt agacatggct gtaaaattag
     4261 gaagaaaagc aaaggaacat ttgcacctgg gagtatgcgg ggagcatggc ggagaccctg
     4321 cctcagttga atttttccac agagtagggc ttgactatgt atcatgctca ccattcagag
     4381 ttcctgttgc aagacttgct gccgcacaag ctgcattaaa agataaaatg aatgttcata
     4441 tcgataaata aaagatgaca aggggacggg ggtagtgtca taaatgacat ccccgtcccg
     4501 atcatattaa tacaggaggc ttacatgacc aatccctcta tctataaaat gagcgtggca
     4561 aaggtctatc ccttttatgt tgccaaggcg gagaaaaaag gacgcacaaa agcagaagtc
     4621 gatgaaatta tccgttggtt aacaggatac agccaagaag aatttcaaga gcagcttaat
     4681 aaacaaacag actttgaaac tttctttttg gaagctcccc tgccaaatcc tgcaagaacc
     4741 ctgattaaag gtgtagtttg cggtgttcga gtagagaacg ttgaagaacc aatcatgcgt
     4801 gaaatccgtt atttggataa actgattgat gaattggcaa aaggaaaagc aatggataag
     4861 attttacgca aataaattca aaataggaag attaggtttg taaataatct gaggtactgt
     4921 ttcttaaaaa aataagaaac agtaccatat ttatatcttg cgattttcag gaactttata
     4981 atatttcgtg ggattctaca aatttacttg tataatataa tggaagatgg aggaggtggg
     5041 catgtggatg aaaagattat tgagcttgta aaatcatcaa aggacaaagg cttaacgctc
     5101 atgcaagaac agtattcagg gctcatgcat tatatagtta gagggatttt acaaaatgag
     5161 agcgacatag aagaatgtat aagcgatatc tgcattaaag tttggaattc catcgagagc
     5221 tattctccgg aaaagggcaa attctccaca tggcttaccg taatatcacg aaatacagcg
     5281 cttaattata tgaaaagcaa gtatactgaa catgacgagc taaaagagga gacagcggat
     5341 aaacactctg tagaagatga tgtccttcgc cgggaacggg cggaggaatt aaaatgtgct
     5401 atacgcacac tttctccgga agaacagcat attttctacc gtaaatatta ttacctgcag
     5461 cgcacatctc aaattgctgc agaacttgga atgacagaac gctctgtcga aggaagacta
     5521 taccgactgc gaaaaaagct gcagaagaaa ttaggaggtg atttcatatg agcgaaaaag
     5581 attataactt tgataaacag ataattgagc agctcagcta cgt
//
LOCUS       DAOSOW010000072         5603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_14605_length_5603_cov_3.736842, whole genome shotgun sequence.
ACCESSION   DAOSOW010000072 DAOSOW010000000
VERSION     DAOSOW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5603
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14605_length_5603_cov_3.736842"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="PLD67_02925"
     CDS             <1..1306
                     /locus_tag="PLD67_02925"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOT21507.1"
                     /translation="FEYNEDAQNKKEDETIISQTACSADLNNSISSTNSKQCRQSLIT
                     VNLSKLDKLVDIVGELVIAESMVESDADIKRLNSESFKKSTRQVRKLTDELQDIVMSL
                     RMVPISGTFNKMHRVVRDMGKELNKDVELVLNGEDTEIDKTIADCISDPIMHLVRNAM
                     DHGIEPEEKRISKGKNKKGKITLSAFNTGGEIHITVEDDGRGFDSEKILEKAEAKGLL
                     TKPASEYTQKEAFQFLMLPGFSTNDDITEYSGRGVGMDVVKSNVEKVGGTVVLESNEG
                     LGSKITFKIPLTLTIVSGMQVVVGNSEFIIPIKSIHQSFKTKKSEIIHNPEQGEMILI
                     REKCYRIIRIHEYFNINTEITNIEDGILIFVESGDNSACLFVDKIIGEQQVVVKPLPI
                     ILNKYNLKESGIYGCSILGDGSINLILDVGNLMESNRYGENN"
     gene            1290..2093
                     /locus_tag="PLD67_02930"
     CDS             1290..2093
                     /locus_tag="PLD67_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOT21508.1"
                     /translation="MVRITDNEFLEYVKYINKNYGIDLSKKKILIEGRLSNLIEKKGM
                     NSFYQYLRSVKNNDVEETMFVNKLTTNYTYFYREEKHFQYLTETILPYEEKNNKAKIL
                     NIWSAGCSSGEEPYTIAMILDDYFKFRINQWKIRIYATDISENVLLKAREGIYSKDSI
                     CKLPDSFIKRYFIKLDDNKYQVVPEIKNYVRFKASNLMAPILEKNRYHVIFMRNVMIY
                     FDLETKNKIINKLYNATRSGGYLMIGHAEIIQREQSKYLYIKPAIYKKE"
     gene            2095..2670
                     /locus_tag="PLD67_02935"
     CDS             2095..2670
                     /locus_tag="PLD67_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HOT21509.1"
                     /translation="MLINKIIVIGASTGGTEAIIKILKELPEDFPPILIAQHMPIGFT
                     KMYADRLNKICKMEVREALNGDRIKQGIVLIAPGDKHMVLKKDDKGYYVSCKKGDKIN
                     GYCPSIDLLFNSAAKIAGNKSVGIILTGMGKDGAEGLLNMKKAGAYTIGQDESTSAVY
                     GMPMAAFNIGAVTVQAKLEDIFEILTKHLTI"
     gene            2737..3111
                     /gene="fliS"
                     /locus_tag="PLD67_02940"
     CDS             2737..3111
                     /gene="fliS"
                     /locus_tag="PLD67_02940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HOT21510.1"
                     /translation="MLNSLEQYKKTSVNTMTKGELLILLFDEAIKKLNQSKLLMEHED
                     YANANIFLTKVRHIFYYLTATLDGKYQISQELSDIYEFFNREIMKASTLKSTKHIDDI
                     LPIIKDLRDTWAEADILSRKKY"
     gene            3121..3480
                     /locus_tag="PLD67_02945"
     CDS             3121..3480
                     /locus_tag="PLD67_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21511.1"
                     /translation="MEELLRNKLEAAKELKKLTSFVNELSLIIDYNRVNSLLDERQQY
                     IDKINVINEKISEVKSKENYVETNEIKKLNKDIGRVFTEIYEIDKVIRKNINTELKSV
                     KEKLNYSETNIVVNIKI"
     gene            3522..5516
                     /locus_tag="PLD67_02950"
     CDS             3522..5516
                     /locus_tag="PLD67_02950"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOT21512.1"
                     /translation="MKTNKSISKKITTTILIVCFSALLITGIIIVKTVSTRFKDNKRQ
                     ILNETALSISNKAEVFFEKYITVVEQMAHDKNLQEFMINAEGYQTLPVTRGFDIATAT
                     TDDIQKADSEIILTAYIAEDDYYLASPTIYTQSEYDVTSKEYYNAVTENKVCITDPYI
                     DAITGGIVITISAPVNVDGEIVGLTAVDIAIDKLINMVDEYELGDTGYFTLLSKNNVI
                     IAHKDKDNLLKKVDEINLSDNIVQSVINKDRSVVEFTYNYDRFIGNSVEIGDTGWQIM
                     SSLPREEFMLNTNQLVLIIIAVFVILLVVLIGVLYVLINKLTKPITDITNITNKLAEG
                     ELDVNIDINSDDEIGVLANSIRKLTERLKLYIIYIDESVGVIDKMAEGHLNMELKNDY
                     AGEFAKLKKSLLNMSNTLKDAIGKIQQSSIAINMNAEQVSTGAQTLAQGTTEQASAIE
                     ELSAEINEIYTTIVNNAEYAENAGSKAMQASKEVERGNMQMREMLSAMDEISNSSSEI
                     GKIIKVIDDIAFQTNILALNAAVEAARAGAAGKGFAVVADEVRNLAGKSAEAAKQTTA
                     LIENSINAINKGTMLAGEAGKSLAGIVDKTNETNELINEIVKASSQQTISVNQIRRGI
                     EQISSVVQENAATAEASAANSEELSGQVLILNDLVKQFTL"
BASE COUNT         2241 a          785 c         1093 g         1484 t
ORIGIN      
        1 atttgaatac aatgaggatg cacaaaataa aaaagaagat gaaacaatta tcagccagac
       61 agcatgttcc gctgatttaa acaattcaat ttcatcaaca aattcaaaac aatgcaggca
      121 aagcttaata acagttaatc tttcaaagct tgacaagctt gtagatattg ttggggagct
      181 tgttattgcg gaatcaatgg ttgagtcgga tgcagatata aaaagattaa attctgaaag
      241 ctttaaaaaa tcaacaagac aagtgagaaa acttaccgat gagctgcagg atattgtaat
      301 gtccttgagg atggttccaa tttcgggcac atttaataaa atgcacaggg tagtaagaga
      361 tatgggaaag gaattaaaca aggatgttga gcttgtatta aacggtgagg atactgaaat
      421 cgacaaaaca attgcagatt gtatttcaga ccccattatg caccttgtca ggaatgcaat
      481 ggaccacggt atagaaccgg aagaaaaaag gataagtaaa ggaaagaaca aaaaaggaaa
      541 aataacctta tctgccttta atacaggggg agaaatacac attaccgtag aagatgacgg
      601 aagaggcttt gattcggaaa aaatattgga aaaggcggaa gccaaaggtt tattaaccaa
      661 accagcaagc gaatatacac aaaaagaagc attccaattt ttgatgcttc ccggattttc
      721 caccaatgat gatataaccg agtattcagg caggggtgtc ggaatggatg ttgttaaatc
      781 aaatgtggaa aaggtgggag gaacggttgt tttagagagc aatgaaggat tgggcagtaa
      841 aataacattt aaaattcctc ttactttaac catagtatca ggcatgcagg ttgtagtggg
      901 aaactctgaa tttataatac ctattaagag tattcatcaa tcatttaaaa ccaaaaaaag
      961 tgaaattatt cataatcccg agcaaggcga aatgatattg ataagggaaa aatgctacag
     1021 aataataaga atacatgaat atttcaacat taatacagaa attacaaata tcgaagacgg
     1081 tatattaatt tttgtggaat caggtgacaa cagtgcctgc ttgtttgttg ataaaataat
     1141 aggggagcag caagttgtcg ttaaacccct tccaataata ttaaacaagt acaaccttaa
     1201 ggaatctgga atttacggct gctcaatatt aggtgacgga agtatcaatt taatattaga
     1261 tgtaggaaat ttaatggaaa gtaatagata tggtgagaat aactgataac gaatttttgg
     1321 aatatgtaaa atacataaat aaaaactacg ggatagacct ttcaaagaaa aagatattaa
     1381 ttgaggggag actatctaat ttaattgaaa aaaagggaat gaacagtttt taccagtatt
     1441 tgagaagtgt aaaaaacaac gatgtagaag aaacaatgtt tgtaaacaag ctgacaacaa
     1501 actatacata tttttacagg gaagaaaagc actttcaata tttaacggaa acaatacttc
     1561 catatgaaga aaaaaataat aaggctaaga tattgaatat atggagtgca ggctgttcat
     1621 caggagaaga accctatact atagcaatga tattggatga ttatttcaag tttcgaatta
     1681 atcaatggaa aatacgtata tatgccactg atatatccga aaatgttctt ttgaaagcac
     1741 gtgaaggtat atattccaaa gacagcatct gtaaacttcc cgattcattt ataaaaagat
     1801 atttcataaa gctcgacgat aataaatacc aggtggtacc cgaaatcaag aattatgtta
     1861 gatttaaagc atctaattta atggctccga tattggaaaa aaacaggtat cacgtaatat
     1921 ttatgagaaa tgtaatgatt tatttcgatt tagaaacaaa aaacaaaatt ataaacaaat
     1981 tatacaatgc aaccaggtca gggggatatt tgatgattgg acatgcggaa ataattcaaa
     2041 gagagcagtc aaaatatctc tatattaaac cggccatata caaaaaggaa taatatgcta
     2101 attaataaaa taatcgtaat aggggcttca acgggaggta ccgaggcaat tatcaaaata
     2161 cttaaagaat tgccggagga ttttcctcca attcttatag ctcagcacat gcctatcgga
     2221 tttacaaaaa tgtatgcgga caggcttaat aagatatgca aaatggaagt aagagaagcc
     2281 cttaacggtg acaggataaa acaagggatt gtattaattg ctcccgggga caagcatatg
     2341 gtgcttaaaa aagacgataa aggttactat gtatcctgca aaaaaggcga caaaataaac
     2401 ggatattgcc cttcaataga tttgcttttt aattcggcgg ccaaaattgc cggtaataaa
     2461 tcagtaggaa ttattcttac cggaatgggc aaagacggag ctgaaggtct tttaaatatg
     2521 aaaaaagcgg gagcgtatac tatcggacaa gatgaaagta cttctgccgt ttacggaatg
     2581 ccgatggctg cattcaatat tggagctgtt acggtgcagg ccaagttaga agatattttt
     2641 gaaattttaa caaaacattt aacaatataa aatttctgac gataatataa gtagcacgaa
     2701 gataaagcaa agattattga gtaaaaagga gaaattatgt taaattcatt ggagcaatac
     2761 aaaaaaacct cagtaaatac aatgacaaaa ggtgagcttc tgattctttt attcgatgaa
     2821 gcaattaaaa aactaaatca aagcaaatta ttaatggagc atgaagacta tgctaatgca
     2881 aacattttct taacaaaagt cagacatata ttttattatt taacggctac attggatggc
     2941 aagtatcaga tatcacagga gttatccgat atatacgaat tttttaacag agaaattatg
     3001 aaagcatcaa cattaaaatc tacaaaacat atagatgaca tattgccgat tattaaggat
     3061 ctaagagaca cttgggctga agcagatata ctttcgagaa aaaagtatta gaggtatgct
     3121 atggaagagt tattaagaaa caaactggag gcagccaaag aattaaaaaa gttaacatcg
     3181 tttgtcaatg aactatcctt aataatcgat tataaccggg ttaattcatt gcttgatgaa
     3241 aggcagcagt atatagataa aataaatgta attaacgaaa aaataagcga agtaaaaagc
     3301 aaggaaaact atgttgaaac aaatgaaatt aaaaaattaa acaaagatat cggcagggtt
     3361 tttacggaaa tttatgaaat cgataaagta attcgtaaaa atataaatac cgagctaaaa
     3421 tcagttaagg aaaagttaaa ttactcagaa acaaacatag ttgtaaatat taagatataa
     3481 acattggaca accataacaa tatatcatat gtggagggat tatgaaaacg aataagagta
     3541 tttccaaaaa gataacaact actattttaa ttgtatgttt ttcggcactg ctaataacag
     3601 gaataattat tgttaaaaca gtcagtacaa ggtttaagga taacaaaaga caaatactga
     3661 atgaaacggc gttaagcata agcaataagg cagaagtttt ttttgaaaaa tatattaccg
     3721 ttgtagagca aatggctcat gacaagaact tacaagaatt tatgattaat gcagaaggat
     3781 accaaacatt acctgtaaca agagggttcg acatagctac ggcaacaact gatgatattc
     3841 aaaaagcaga ttcagaaata attcttactg cttatattgc agaagatgat tattatttgg
     3901 catctccaac catctataca caatccgaat atgatgttac ttccaaagaa tattacaatg
     3961 cagttacaga aaataaagta tgcattaccg acccttatat tgatgctatt acaggaggaa
     4021 tagttataac catatctgca ccggttaatg tagacggtga aattgtaggc ttgacggcag
     4081 tggatatagc cattgataaa ttaataaaca tggttgatga atatgagctg ggagacacag
     4141 gatatttcac attgttatca aaaaataatg ttataattgc ccataaagat aaggataact
     4201 tattaaaaaa agttgatgaa ataaacttaa gcgataatat cgttcaatct gttatcaata
     4261 aagacaggtc cgtagtagaa ttcacatata attatgacag gttcatcgga aacagtgtgg
     4321 aaatcggaga tacggggtgg cagattatgt catcgcttcc gagagaagaa ttcatgttga
     4381 atacaaatca gttggtatta attattatag ctgtattcgt aatattattg gttgtactga
     4441 ttggtgtttt atacgtatta ataaataaat tgacaaaacc gataacggat ataacaaaca
     4501 ttaccaacaa attggctgaa ggcgaattag atgtaaatat tgatataaac agcgacgatg
     4561 aaatcggtgt acttgcgaac tctataagaa aactgacaga gcggttaaaa ttatatatta
     4621 tatatattga tgaaagcgtc ggagttatag ataaaatggc tgaaggacat ttaaacatgg
     4681 agcttaaaaa tgattatgca ggtgaatttg caaaattaaa aaaatctttg ttaaatatgt
     4741 ccaatacgct aaaagatgca ataggaaaaa tacaacaatc ctcaattgca atcaacatga
     4801 atgccgagca ggtttcaacg ggagcgcaaa cattggcgca aggaacaacg gagcaggcaa
     4861 gtgcaataga ggaattatca gcagaaatca acgaaattta cacaactatc gtaaacaatg
     4921 cagagtatgc agaaaatgca gggagcaagg caatgcaagc ttccaaggaa gttgagcgcg
     4981 gcaacatgca aatgcgcgaa atgctttcag caatggatga aataagcaat tcatcaagcg
     5041 aaataggaaa gataataaaa gttatagatg atatagcatt ccaaactaac atacttgcat
     5101 taaatgcagc tgtagaagct gcaagagctg gtgcagcagg caagggattt gcggttgtag
     5161 cggacgaagt aagaaatttg gccggtaaat cggcggaagc cgccaaacag acaaccgcac
     5221 tgattgagaa ttcaataaat gcaatcaaca aaggaacaat gctggcaggc gaagcaggca
     5281 aatcattggc aggtattgtc gataaaacaa acgaaacaaa tgagctgata aatgaaattg
     5341 taaaagcatc atcacagcag actatttcag taaatcaaat aagaagaggc attgagcaaa
     5401 tatcatcggt agttcaggag aatgcggcaa cagcagaggc aagtgcagcc aacagcgaag
     5461 aattatcagg acaagtattg attctcaacg atttagtaaa acaattcact ctataagctt
     5521 actttctgta caatataaaa tacaaccata aagctatggt tgtattttaa tttatttcat
     5581 aatttgttta atttattgga att
//
LOCUS       DAOSOW010000073         5593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_14635_length_5593_cov_3.454135, whole genome shotgun sequence.
ACCESSION   DAOSOW010000073 DAOSOW010000000
VERSION     DAOSOW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14635_length_5593_cov_3.454135"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            444..1370
                     /locus_tag="PLD67_02955"
     CDS             444..1370
                     /locus_tag="PLD67_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HOT21513.1"
                     /translation="MDSITQDMKFRLSLIKYAEKYGVTRAAIKYKTNRQYIYRWKRRF
                     DGSIESLRNKSRRPHHHPNQHTEQEIKLINDMRKRNMHEGLVVFWVKLRQRGYTRSIP
                     GLYRFLKKQQLMAVKPPNPKYVPKPYEQMSYPGQRVQVDVKHVPAVCLVGDAKGEKFY
                     QYTAIDEFSRFRYVEAFNEASTYTSALFVKNLTKAFKFNIECIQTDNGFEFTKRFKSD
                     KPSLFEKTLSELGIKHKLIRPFTPRHNGKVERSHRKDNERFYAVRSFYSFDDFSKQLS
                     IYNKRDYNNFPMRPLLWRSPKQVLDDFFSSSV"
     gene            complement(1558..1704)
                     /locus_tag="PLD67_02960"
     CDS             complement(1558..1704)
                     /locus_tag="PLD67_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LDCC motif putative metal-binding protein"
                     /protein_id="HOT21514.1"
                     /translation="MFKFIKNWLDRLAKENEKNFGTDRLDCCGLNKPKNTNTQSSNSN
                     TDRK"
     gene            complement(1866..3233)
                     /locus_tag="PLD67_02965"
     CDS             complement(1866..3233)
                     /locus_tag="PLD67_02965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT21515.1"
                     /translation="MVKISKQLRKYFITISILSIAFITITSNISINFFFSGYIRDSRS
                     RDDLKVLKYVESVFSEYEEINDNSSMNLMHYAFSEDVVIQIKDTNSSIMFNSSAFGTI
                     IGITDEYSKNEEKFSFRSYPFIIDDEQVGTIDIGRPKSIIANIEDEKFLITINSIFAA
                     ASLLTLIIALRSSAHLSKKFLNPIYRIQENAKLIEQGKYKNINEVETNTSELHELSVS
                     VKELAERLHHQETLKRRMTTDIAHELRTPLAAVQSHIEAFMDGVWEPDYERLSVIHGE
                     VIRLTKLISELSNLSIVEGDEIKLKLSTVDLSEILNDIVDSYEPMFSDKNISVNKNIQ
                     NDVNMMGDIDYLKRIFINILSNAFKYTNENGTVSVSLEQIKNQIRITVKDTGIGIPKE
                     DLKHIFERFYRSDLSRNRQTGGTGIGLTITKALVEAHGGTIKIDSEVGKGTNVIVEFV
                     NLNHH"
     gene            complement(3235..3918)
                     /locus_tag="PLD67_02970"
     CDS             complement(3235..3918)
                     /locus_tag="PLD67_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT21516.1"
                     /translation="MKEKILVVDDEENLLNVIKDYLLRESYEVYTANRGNKAIELFRK
                     IEPDFIILDLMLPDISGEEICKLIRKESNVPILMLTAKSSEDDKVTGLYIGADDYLTK
                     PFSPRELVGRVRAILRRTKGTAADVLEFNDKDLYIDIPKHIVKKAGESIKLTPNEFKI
                     LSVLAQNPHKVFTRSHLVSLAFGYDFEGFDRTVDTHIKNLRQKIEDNVKEPKYIVTVY
                     GVGYKFEGV"
     gene            4351..>5593
                     /locus_tag="PLD67_02975"
     CDS             4351..>5593
                     /locus_tag="PLD67_02975"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HOT21517.1"
                     /translation="MNEIQLNYIVQAILGGASGYITNDYAINMLFKEYTPLKLGGVIK
                     KTRNEFIENLSSLVENDIINKEKLLEIFESPEFKFKFEKLTCDFYENCLYETVGNGKF
                     SDIDGFDSSLKGLDNFVIEILNNNLESIIGLIADNFDAEYFLTNSQSEKIADSIYNSI
                     KDVIENTDAVENILKNIYENNKELNLSHIFGSNMQTTDNLINKLTEIVADNAEHSELF
                     AGFNAALNEALKVFYDKQIKDVIKFDKEIINSFISYIEKNDIILKLCRSLFSYGRSID
                     KSLYSIIDPAFESSLKAYIENNLPFVTEKLVSYVQKNSILIDRIIEDTLDEVVSESEG
                     FRKRMLHVIKNTYFNNLSKKYSIVDKIISYIRQFTEPEKLSIDISKNIIERLDNITVS
                     DIISETENNFNLYKVYEIVAGF"
BASE COUNT         1875 a          938 c          841 g         1939 t
ORIGIN      
        1 gattaaatga gctgctgtaa atcgtaatga aatcgtaagc ggcggcgcgg tcagcggtca
       61 ccgcatcgtt ggatacgcca aacagcagag tcattcgcag agatatttca aatccagatt
      121 tattacaata gagatataaa aagactcata gcaagcgcca gtaaaagcgc tcacaatgag
      181 ccacttttat gtattatacc acaggcattc gcaaaattca atatactttg gcgaacgcga
      241 aataattttg ttgtaggatt acaatacatg tgttacatta aaacatttta tcctatggat
      301 ttgtggaaaa ctctgcgagt ttaccacaaa cccacaggat ctgctgctgc tactactaaa
      361 atctaataaa taaatagcga gaattactct ctttatgtta tacttttttt accacaaaaa
      421 actaacaaaa ggagcttctc gctatggata gtataacaca agatatgaaa tttcgtctat
      481 cattaattaa atatgctgaa aaatatggtg taaccagggc cgccattaag tataagacaa
      541 acaggcaata tatttatcgc tggaaacgtc gttttgacgg ctctattgag tctttacgca
      601 ataaatcaag aagaccacat catcatccaa atcagcatac cgaacaagaa attaagctta
      661 tcaatgatat gcgtaagcga aacatgcacg aaggtcttgt tgtattttgg gttaagctta
      721 ggcaacgtgg ctacacgaga tctatccctg ggttgtatcg cttcttgaaa aagcaacagc
      781 ttatggctgt taagccacct aatcctaaat atgttcctaa accttatgag cagatgtctt
      841 atcctggtca acgcgttcaa gttgatgtaa agcatgtgcc ggctgtctgt ttggtcggtg
      901 atgctaaggg tgaaaagttt taccagtata ccgctattga tgagttttct cgttttagat
      961 atgttgaggc gtttaatgaa gccagtactt atacttccgc attatttgta aaaaacctta
     1021 ctaaagcttt taagttcaat attgaatgca tccagaccga taatggcttt gaatttacca
     1081 aacgctttaa aagtgataag ccttcattat ttgaaaaaac tctttctgaa cttggtatta
     1141 aacataagct cattagacct tttacaccaa gacataatgg taaagttgaa cgcagtcatc
     1201 gcaaggataa cgaaaggttc tatgccgtta gatctttcta ctcttttgat gatttctcta
     1261 aacaattaag tatatacaat aaaagagact acaataattt tcctatgaga cctttattat
     1321 ggcgctcacc taaacaagtt ttagatgatt tttttagctc cagtgtgtaa catatgtttg
     1381 acaaacctac atcaataaga aaaagcagcc aataatctaa atgttgactg caacgtatag
     1441 aaccttattt ttatatcatt caagttgtaa aatctgtcat aattttaaag aaatccatgg
     1501 cgcttaaaat ctgcatagca gataatacct accaaactct ttcccaatca ttgcatttca
     1561 tttcctatca gtattgctat tagaagattg agtatttgtg tttttaggtt tatttaaacc
     1621 acagcaatcg agcctgtcgg ttccaaaatt cttttcattc tccttagcta atctgtctaa
     1681 ccaatttttt atgaatttaa acataaatat tacctccttt tttagctgtc tatatagata
     1741 taaagcataa aaatgccata ccccggtggg gggtgtatga tgaatattaa caatactttg
     1801 tgaagaagtt atgaagatat atatattcta ttatgtcatc ctgagcacaa gcgaaggatc
     1861 taatattaat gatgattcag attaacaaat tcaacaatta catttgttcc ttttccaacc
     1921 tcgctgtcta tttttatggt accaccgtga gcttcaacca atgcctttgt aattgtaagc
     1981 ccaatgccgg ttcctccggt ttgacgattc cttgataaat cgctcctgta aaacctttca
     2041 aaaatatgtt ttaaatcttc cttagggata ccaatacccg tgtcttttac tgttattctt
     2101 atctgatttt ttatttgctc taatgaaaca ctgacagttc cattttcatt agtatactta
     2161 aatgcatttg aaaggatatt aataaaaatt cgttttaaat aatctatatc tcccatcata
     2221 tttacatcat tttgaatgtt tttatttaca gaaatatttt tatctgaaaa cataggctca
     2281 taactgtcta caatatcatt taaaatttcc gataaatcaa cagtgcttaa ttttaatttt
     2341 atttcatcgc cctctactat tgataaattg gataattcac tgattaattt agttaatctt
     2401 attacctcgc cgtgaataac agaaagccgc tcataatcag gctcccatac cccgtccata
     2461 aaggcctcga tatggctttg aacggcagct aagggtgttc ttaactcatg ggctatatcc
     2521 gtagtcatcc ttcgctttaa tgtttcctga tggtgcaacc tttcggcaag ctcttttaca
     2581 gatacggaca attcatgcaa ttcggatgta tttgtttcca cttcatttat atttttatat
     2641 tttccttgtt ctatcagctt agcattttct tgtattctat atattggatt taaaaacttc
     2701 tttgataaat gagcacttga acgcaatgct attattaatg tcagcaatga agccgctgcg
     2761 aatatactgt ttatggtaat taaaaatttt tcatcctcaa tattggctat tatgctttta
     2821 ggtcgtccaa tatcaatagt tccaacttgt tcatcatcaa taataaacgg atagcttcta
     2881 aacgaaaatt tttcttcatt tttgctgtat tcgtctgtaa ttcctatgat tgtaccaaat
     2941 gcgctgctgt taaacattat actgctattc gtatccttaa tctgtataac aacatcctcg
     3001 ctgaaagcat aatgcattaa attcattgaa gaattatcat ttatttcttc gtattcactg
     3061 aatacacttt ccacatactt aagaactttt aaatcatctc tgcttcttga atcccttata
     3121 tagccggaga agaaaaagtt tatgcttata ttagaagtaa ttgtaataaa tgcgattgat
     3181 aatattgaaa ttgtgataaa gtacttcctt aattgcttgc ttattttaac catcttatac
     3241 accttcaaac ttatatccga caccgtagac agttacaata tatttaggtt cctttacgtt
     3301 atcttctatt ttttgacgca aattttttat gtgtgtatca acagtgcgat caaatccttc
     3361 aaaatcatat ccgaaagcca gactgactaa atggctcctg gtaaatactt tatgaggatt
     3421 ttgtgctaat actgataata ttttaaattc attaggtgtc agttttattg attcgcctgc
     3481 tttcttgact atatgtttag gtatatcaat atacaaatct ttatcattaa attctaaaac
     3541 atcagctgca gtgccctttg ttcttctaag aattgccctt accctgccga ctaattccct
     3601 tggactgaaa ggtttggtta aataatcatc tgcaccgatg tataaaccgg taaccttatc
     3661 atcttcgctg cttttagcag taagcatcaa aataggaaca tttgattcct ttcttataag
     3721 tttacatatt tcctctcccg aaatatcagg aagcatcaaa tcaagtataa taaagtccgg
     3781 ttctatttta cgaaataatt cgatagcttt attcccgcga ttagccgtat aaacttcata
     3841 ggactctctt aaaaggtaat cctttattac atttagtaaa ttttcctcat catcaacaac
     3901 taagattttt tctttcattt ttcaccttta attatcaatt atcaatgtta acaatacctt
     3961 aaatttagta tattgctcat attatactct ttaaatccaa tattaaaaca tgtggtttat
     4021 gaagaaaatt aattaatttg tttttcttca ttatttctac ataatttact tgtataatct
     4081 gtatagtttt caaacaaaag tgattgttat aatacttgtc ccccctaaac agtgattaac
     4141 aatcgaataa taagaccatt aaggtcttat ttttttatgt aactctatat aataacaatg
     4201 ataaagcagg aatgtgaaga aattatgaag tttactttct catataacat aataaatgtg
     4261 caaaactagt caaaggaaaa aagttttttg caatttaaaa tattaaatgc tataatccct
     4321 aaaaaggtat ggaatgaaag gtagacttca atgaatgaaa tacagctgaa ttacatagta
     4381 caggcaatat taggaggagc ctccggttat attacaaatg actatgcaat taatatgctt
     4441 tttaaagaat atactccctt gaaattaggc ggcgttatta aaaaaacgcg taatgaattt
     4501 atagaaaatt tgagctcact ggttgaaaat gacattataa ataaagaaaa gcttcttgaa
     4561 atttttgaaa gccctgagtt taagttcaaa tttgaaaaac ttacctgtga tttctatgaa
     4621 aactgtcttt atgaaactgt gggcaatggc aaattttcgg atattgacgg gtttgactcc
     4681 tcattgaagg gtttagataa ttttgttata gaaattctta ataataattt ggaaagtata
     4741 attggattaa ttgcagataa ttttgatgca gaatactttc tgacaaattc tcagtcggaa
     4801 aaaatagcag attccattta taattctatt aaagatgtta ttgaaaatac tgatgcagtt
     4861 gaaaatattt taaaaaatat ttatgaaaac aataaagaat tgaatctttc tcatatattt
     4921 ggctcaaata tgcagacgac agataattta ataaataagc ttacagaaat tgtagcagat
     4981 aatgctgaac attctgaatt atttgcagga tttaatgctg ctttaaatga agcattgaag
     5041 gttttttatg acaagcaaat taaagatgtg attaagtttg ataaggaaat tataaattcc
     5101 tttatttcat acatagaaaa aaatgatatt attcttaaat tatgccgctc tttattttct
     5161 tacgggagaa gcatagacaa aagcttgtat tctataatag accctgcatt tgaaagcagt
     5221 cttaaagcat atatcgaaaa taatcttccc tttgtaactg aaaaattagt cagctacgtt
     5281 caaaaaaaca gtattcttat tgacaggatt atagaagata ctttagacga agtagttagt
     5341 gagtcggaag gctttagaaa aagaatgctg catgtgatta aaaacaccta ttttaataat
     5401 ttaagcaaaa aatacagcat tgttgacaaa attatttcat atataaggca gtttacagag
     5461 cctgaaaagt tgagcataga tataagcaaa aatataattg agcgtttgga taatataacc
     5521 gtaagcgaca ttatctcaga aacagaaaac aactttaacc tttataaggt ttatgaaatt
     5581 gttgcaggct ttt
//
LOCUS       DAOSOW010000074         5587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_14661_length_5587_cov_3.526211, whole genome shotgun sequence.
ACCESSION   DAOSOW010000074 DAOSOW010000000
VERSION     DAOSOW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5587
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14661_length_5587_cov_3.526211"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1899)
                     /gene="uvrB"
                     /locus_tag="PLD67_02980"
     CDS             complement(<1..1899)
                     /gene="uvrB"
                     /locus_tag="PLD67_02980"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOT21518.1"
                     /translation="MGEFKIKSEYTPTGDQPEAIEKITEGLKKNYKYQTLLGVTGSGK
                     TFTMANIIEKIQKPTLVLAHNKTLAAQLCSEFKEFFPDNAVEYFVSYYDYYQPEAYVP
                     SSDTFIEKDASINDEIDKLRHSATAALFERKDVIIVASVSCIYGLGSPIDYENLVISL
                     RPGMVKDRDEIIRKLVEIQYKRNDIAFTRGTFRVRGDTVEIFPASSSENAIRVEFFGD
                     EIDRITEINTITGEILGRRNHISIFPATHYATTEENVSRAVESIKEELQERLKQLESE
                     NKLLEMQRLEQRTNYDIEMLQEMGYCNGIENYSRHINNLKPGARPYTLLDYFPKDFLM
                     IIDESHMSIPQVRGMYNGDRARKTTLVEYGFRLPSALDNRPLKFDEFESLINQVIFLS
                     ATPGPYEIEHSQNVVEQIIRPTGLIDPPIYVRPVENQIDDLINEIRKSTDKNQRVLVT
                     TLTKKMAEDLTAYLKSTGIKVRYLHSDVDTLERMEIIRDLRIGEFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSETSLIQTVGRAARNIDGRVIMYADRITGSMERAIKET
                     NRRREIQDKYNKLHNITPKSVIKGVRDVIEATKAAEEESQYITKGKDISISEYIIELE
                     KEMRIAAEN"
     gene            complement(2129..2614)
                     /locus_tag="PLD67_02985"
     CDS             complement(2129..2614)
                     /locus_tag="PLD67_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfcE family phosphodiesterase"
                     /protein_id="HOT21519.1"
                     /translation="MKILVMSDSHNVILNSQIELIKKEGVFDLLIHCGDKFNDAQRFA
                     EELHIENVLNVPGNCDFSLIGQNTTIIKEIEGKKFIITHGHIHNVKLNLNKLKDFAKK
                     NKADIVVYGHTHKSQNEFIDNILYFNPGSTIFPKDGRASFGIIEITEETINGRIVPLE
                     N"
     gene            complement(2592..3209)
                     /locus_tag="PLD67_02990"
     CDS             complement(2592..3209)
                     /locus_tag="PLD67_02990"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HOT21520.1"
                     /translation="MKRRIILSTGNTHKINEIKGILKDMPFEVISKNDLGYEDFDVVE
                     DGNTLEENALKKAGELHKLTKGIVIADDTGLFVDALNGEPGIYSSRYAGEHASYAENN
                     KLLLNKLKDVPKEKRTAYFKTIIAVILEDGSRLTAEGICRGYIAFQERGKRGFGYDPL
                     FVVEGTDKTFSEMSEEEKNKISHRAKALKNLKDKLEEIIEDFGDE"
     gene            complement(3525..>5587)
                     /locus_tag="PLD67_02995"
     CDS             complement(3525..>5587)
                     /locus_tag="PLD67_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOT21521.1"
                     /translation="LLLILITLVQHSAPDAYAEDSIKIIIDGKRIKPDVDPYISNDRT
                     LVPIRVIAEELDSVVEWDNDNRAVHISKEDIHILLRIDSYLVEYTNDNETTYTIIDVV
                     PQITGDRTFVPLRLISNALGVGIEWDNDKRTVYIDSSESSEVTKFFDVEISSVKPGQT
                     ITGTTELYTKINNEIPKGAKEIKYLLLDRDTAKGFVIAGGSNINNPYEWVPAMEDNGQ
                     KVLVSAFYDAQGNFLAGDSIPVTVKINPQIELKGIREGQLITAHSVPLSTKLNFSASY
                     VKYEMINPDNGAVYLSPGVDPEQSFTMIPVMEDNGNMSVRVIAYDTKGNAYKGEYVNI
                     GIDVDRYLYLGGVKHGQTIDGSVTLLAQRNFNVTDTEYIMVDNSTGEETLLFKSGYGS
                     YSWFPGPEDAGSKDLYVRVKDTAGNTHVSSRVTVNVVGTPKLMLQGVGPGQVLTQAAD
                     LNIKTNVNLDSIKYILTNVRTGNEIIISEKSTGVIIPEKGDDGTWTIRAQGTYGGRTI
                     KSEEVKFTIYTGQLYSAKPVIEKDKYLDLVSGLAVNTRKTTGMSAALQVAQAILETGW
                     GQSVPVDKYDGKFSYNLFGIKGEGTKGSVTSNTWEEYNGVAFRIDAEFRAYNNEKESW
                     QDHKDLLLLRERYAPFREVMYDSTKGAWALKRCGYATDSQYAIKLIDIIERYDLKELD
                     EVTI"
BASE COUNT         1476 a         1200 c          883 g         2028 t
ORIGIN      
        1 gttttctgca gctattcgca tttccttttc taattctata atatattcag atatactgat
       61 atccttaccc tttgtgatat attggctttc ttcctctgcc gccttggttg cttctattac
      121 gtcacgcaca cctttaataa cggatttcgg agtaatgttg tgtagcttat tgtacttatc
      181 ctgaatttcc cttcttctgt ttgtctcctt tattgctctt tccatggagc cggttattct
      241 gtctgcatac ataataaccc ttccgtcaat atttcgagct gcccttccca ctgtctgtat
      301 aagagatgtt tccgagcgaa ggaatccttc cttgtctgca tcaagtattg caaccaagga
      361 tacttcaggt aaatccaatc cttcccttaa aaggtttatt cccacaagca catcaaactc
      421 accgatgcgc aggtctctta ttatttccat tctttccaat gtatctacat cagaatgcaa
      481 atatcttacc ttaataccgg tactttttaa ataggctgtc aaatcttcag ccattttctt
      541 tgtaagagtt gtaaccaata ccctttgatt tttatcggtt gattttctga tttcatttat
      601 aaggtcatct atttgatttt ccaccggtct tacatatatg ggcgggtcta tcagtcccgt
      661 aggacgaata atctgctcaa caacattttg agagtgttct atttcgtagg gacccggtgt
      721 tgcgcttaaa aatataacct gattaattaa actttcaaac tcatcaaact tcagcggtct
      781 gttgtcaaga gccgaaggaa gtctgaaccc gtattccacc aatgttgttt tccttgccct
      841 gtcaccgttg tacatgcctc ttacctgagg aattgacatg tgagactcat ctattatcat
      901 caaaaaatcc ttgggaaaat aatcaagcaa tgtataaggt cttgctcccg gcttaaggtt
      961 atttatatgt cttgaataat tttcaattcc gttgcagtaa cccatttcct gcagcatttc
     1021 aatgtcatag tttgttcttt gctccaatcg ctgcatttcg agcagcttgt tttcgctttc
     1081 aagctgcttc aatctttcct gaagttcttc ctttatactt tcaacagctc tgctcacatt
     1141 ttcttcagta gtggcatagt gggttgcagg gaatattgaa atatgatttc ttctgcctaa
     1201 tatttcgcct gttatcgtat taatttccgt aatcctgtct atttcatctc caaagaactc
     1261 cacccttatg gcattttcag aagacgaagc ggggaatatc tccacggtat cccctcttac
     1321 cctgaatgta cctctggtaa atgctatatc atttctttta tattgaattt caacaagctt
     1381 tcttattatt tcatctctgt ccttaaccat cccgggtctt agagatataa ctagattttc
     1441 ataatctata gggctaccca gtccgtaaat gcacgaaaca ctggcaacaa ttataacatc
     1501 ttttctttca aaaagtgcag cggttgcgga gtgcctcaat ttgtctattt catcatttat
     1561 ggatgcgtcc ttctcaatga atgtatcact gcttggcaca tatgcctccg gctgataata
     1621 atcataataa gatacaaaat attcaaccgc attatcagga aagaattcct tgaattccga
     1681 gcatagctga gccgccaatg ttttgttatg agctaataca agagttggtt tttgtatctt
     1741 ctcaattata tttgccatag tgaaagtttt ccctgagccg gtaactccaa gaagtgtttg
     1801 atatttataa tttttcttca acccttcagt aattttttct attgcttcgg gctggtcacc
     1861 ggttggagta tattcggatt taattttaaa ttcacccatg aagtgctcct tttgtctaaa
     1921 cgtatgttcg ctccattata taatttttgt attgttaagt caacaaaatc tgcaagcgaa
     1981 aataagcata aaaaatatgc aaaaagattg taccagtaga ggaacgggga cacataaatg
     2041 tccccgtgtt atgagattct tcgctgcgct cagaatgaca aagcataatc atgagattct
     2101 tcgctgcgct cagaatgaca ctcaagtcct agttttccaa tggtactatt ctgccgttta
     2161 tagtttcttc agttatttct attataccaa agcttgcacg tccgtccttt gggaagattg
     2221 tactgcccgg attaaaatat aatatattgt cgataaattc gttttgtgac ttatgagtat
     2281 gtccgtatac tacaatatct gctttgtttt tcttggcaaa gtccttcagc ttatttaaat
     2341 ttaatttcac attatgtata tgtccatgag taataataaa ttttttgcct tcaatttctt
     2401 tgattattgt tgtgttctgg ccaataagac tgaaatcaca gtttcccgga acgtttaaaa
     2461 cattttcaat gtgcagttct tcggcaaatc tttgtgcatc attgaattta tcgccgcaat
     2521 gaattaaaag gtcaaaaacc ccttcctttt ttatgagttc tatttgagaa tttaatatga
     2581 cattatggct gtcactcatc accaaaatct tcaataattt cctccaactt atcttttaaa
     2641 ttttttagtg ccttagccct gtgactgatt ttattttttt cttcttccga catttccgaa
     2701 aaagttttat ctgttccttc cacaacaaat aacgggtcat atccaaaacc ccttttgcct
     2761 ctttcctgaa atgctatgta acctctgcaa atgccttctg cagttaaccg gctgccatct
     2821 tctaaaataa cagcaataat tgttttaaaa taagcggttc ttttttcttt cggaacatct
     2881 tttaacttat tcaggagcag tttattgttt tcagcatagc ttgcatgttc tcccgcatac
     2941 ctggaagaat atattccggg ttctccgttt aaagcatcta caaataatcc cgtatcatcg
     3001 gctatgacta tcccctttgt gagtttgtga agctctccag ccttttttag agcattttcc
     3061 tctaaggtgt tgccatcctc cactacatcg aaatcttcat atcccaaatc atttttagat
     3121 atcacttcaa agggcatatc ctttaaaata ccttttattt cattaatctt atgtgtatta
     3181 ccggttgata aaattattct tctcttcatt catccttccc cgcttaattt ctttagtttg
     3241 tcgtacccta tatttctctg tgactgccct aagtatctta aaatatcgtc attgctatga
     3301 ataagatacg tgtcattctg agagctttag ctcgaagaat ctcattttta ccatgagatt
     3361 ctttgcttac tctcaggatg atatatctta tatttattat atcctaattg gatttattta
     3421 acaattatat tgtaacccga aggaagggga caaaccttca aaatcagttt taaataataa
     3481 tttttgaagg atgtccccaa ctttttattg ctataataat aactctatat tgttacttca
     3541 tccagctctt ttaaatcata tctctcaata atgtctatca gtttgattgc atactgtgaa
     3601 tctgttgcat atccgcatct ttttagcgcc caagctcctt ttgtgctatc atacataact
     3661 tctctgaaag gtgcatacct ttctcttagc agcaataagt ccttgtggtc ctgccagctt
     3721 tctttttcat tgttgtatgc tctgaactca gcatctattc tgaatgcaac gccgttatat
     3781 tcttcccagg tattggaggt tacggagccc tttgtccctt cacctttaat tccaaaaaga
     3841 ttgtatgaaa atttaccatc atatttatcc acgggtacac tttgtcccca gcctgtttcc
     3901 aatatagcct gtgcaacctg caatgcggcg gacatgcctg tggtttttct tgtatttacg
     3961 gctaaaccgg aaaccaagtc caaatatttg tctttttcaa ttacgggttt tgctgaatat
     4021 aactgacccg tgtaaattgt aaatttaacc tcttctgatt ttattgtcct accgccgtat
     4081 gtcccttggg ctcttatggt ccatgtgccg tcatcacctt tttcgggaat aattactccg
     4141 gtgcttttct ccgagatgat aatttcatta ccagtcctaa catttgtaag aatatatttg
     4201 atactgtcta agtttacatt agtttttata tttaggtctg cagcttgagt taatacttgt
     4261 ccgggtccaa cgccttgaag catgagcttt ggtgtgccga ctacattaac cgtcactctg
     4321 ctgcttacgt gagtgttgcc tgccgtatct tttaccctta catacaaatc cttgcttcct
     4381 gcatcttcgg gtccagggaa ccaggaatat gaaccataac ctgatttaaa caatagagtt
     4441 tcttcaccgg ttgaattgtc aaccattata tattcggtat cagttacatt gaaattcctt
     4501 tgtgccagta aagttacaga gccgtcaata gtttgtccat gtttaactcc gcctaaataa
     4561 agatacctgt caacgtcaat tccaatgttt acatattcgc ccttatatgc atttccctta
     4621 gtatcgtaag caataaccct tacggacata ttgccgttgt cttccataac gggaatcata
     4681 gtgaatgatt gctccgggtc aacaccggga gacagataga cagctccgtt atcaggattt
     4741 atcatttcat attttacata tgatgccgag aaatttaact ttgtgcttaa aggtaccgaa
     4801 tgagctgtaa ttaattgacc ttcccttatt cctttcagct ctatctgagg atttattttt
     4861 accgtaacag gtatagaatc gcctgccaga aagttaccct gtgcatcata aaatgctgaa
     4921 acaagtactt tctgcccatt gtcttccatg gcaggaaccc attcataagg gttatttatg
     4981 ttgctgcctc ctgcaataac aaatcctttt gcagtatctc tatccaaaag aagatattta
     5041 atttcttttg ctcctttcgg tatttcatta ttaatttttg tatacagctc agttgttccg
     5101 gtaatggttt gtcccggttt taccgatgaa atttccacat caaaaaactt cgtaacttcg
     5161 gaactttccg atgaatctat atataccgtt cttttatcat tgtcccattc tattcccaca
     5221 cctaaggcat tacttattaa acgcagggga acaaaggtac ggtctcccgt tatttgagga
     5281 accacatcaa taattgtata agtagtttca ttgtcatttg tatattcaac taagtagctg
     5341 tcaattctaa gaagtatgtg tatatcttct ttagatatat ggactgccct gttatcatta
     5401 tcccattcaa cgacagaatc caattcttca gctataaccc ttatgggtac caatgtcctg
     5461 tcattgctta tataaggatc cacatcgggt tttattctct tgccatctat tattatttta
     5521 attgaatctt cagcataagc gtcgggtgcc gaatgttgaa ccaaagtaat caatattaac
     5581 aagagaa
//
LOCUS       DAOSOW010000075         5491 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_14968_length_5491_cov_3.596026, whole genome shotgun sequence.
ACCESSION   DAOSOW010000075 DAOSOW010000000
VERSION     DAOSOW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14968_length_5491_cov_3.596026"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1773)
                     /locus_tag="PLD67_03000"
     CDS             complement(58..1773)
                     /locus_tag="PLD67_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21522.1"
                     /translation="MLKRFIAYYKPVKIIFITDMICAFAVAVCDLFYPMITRNIINIY
                     VPNQEFQLMIKWLVVLGFIYILKFGLNYYITYYGHIMGVKMQAHMRKDIFEHLQDLPF
                     VFFDENKTGTLSSRIINDLMDISELAHHGPEDLFISLVMLIGSFIMLSTINLPLSLIV
                     FSAVPLLLIVAMKLRLKMEDAFMETRVTIGEVNATIENSISGIRVSKAFSNKENEIEK
                     FEKNNSRFQEARRKAYKVMAEFHVGSSFIIDILNIVVLSSGAIFFFIGIINFGDFAAF
                     LLYTGNFLNPIRKLVNFVEQYQSGSSGFKRFIEIMDKDVEKDDPGAADIYDVKGEISF
                     DDVSFAYDDNKEILKDITFDIEAGKTVAIVGPSGGGKTTLCHLIPRFYEIEEGSISID
                     NIDIRKFTRKSLRKNIGIVQQDVFLFTGTILENIQCGKLDATKEEVYKAAKNANIHDF
                     IMSLPEGYDTYVGERGVKLSGGQKQRISIARVFLKNPPIIILDEATSALDNATEFLIQ
                     KALDELSKGRTTIVVAHRLSTIKNADEIIVLTDQGIQEKGRHQELLDKKGMYAGLYNS
                     QFINQ"
     gene            complement(1777..2049)
                     /locus_tag="PLD67_03005"
     CDS             complement(1777..2049)
                     /locus_tag="PLD67_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21523.1"
                     /translation="MSKRPGYAATAAILGIIMHNASVHKIFERIFKYSAHKNAKAAMI
                     ISLILIAIISYFIRLGFILFVLLDFASIILFTAAALIYSKFENRQE"
     gene            2435..3931
                     /locus_tag="PLD67_03010"
     CDS             2435..3931
                     /locus_tag="PLD67_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT21524.1"
                     /translation="MKKIIAMLLIAMLISACTAQENTPEKADVKTELTMGYTTEPEGL
                     DPHRTAAASTFTVTNNIYDTLVGVTPQWEIVPRLAKEWVISDDGMEITFSLRDDVYFH
                     NGRQMTAKDVEYSFTRLKDAESPRARDYENIVKIEVLNDYEIKFITAELDVELLKSFV
                     YPWAAVVPEEEVANLKTEPVGTGAFTLEEWVPQQHLILKRNENYYGDEIKLETVKMVL
                     IPDATGMMAGFQVGDLDIIPLTGDQVTMVEGNENYKIISEPMNAVQIMSLNTKNEYLS
                     NEKVRQAMAMAINKDEVIEASMFGYGAKIGSHLPPTSPDYYDTNNIIEYNPEKAKELL
                     KEAGYENGFEVDMSLPKNYQLHVDAGQVIADQLGRIGIKVNIQLVEWGTWLSEVYGAK
                     NFDTTVVGHTGRLDSYAFLSRYKSDSNDYISLTTGEVDALLEEALKELDENKRKEIYK
                     DIQVILANKLPAIYLQTPHTMLALQKNVEGMKIFPIDFYDYKDVYFTE"
     gene            3956..4915
                     /locus_tag="PLD67_03015"
     CDS             3956..4915
                     /locus_tag="PLD67_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT21525.1"
                     /translation="MFNYVLDRLKSSIVVLFVVSIITFFVLMIIPGNPAQLILGADAT
                     PEAIQELHVAMGLDRPLYQQYFSWLLDLLKLDMGKSYVYGESVTVLIAKSIPVTFSLS
                     IFAMIMAAFIAFLFGMISAIKKDSIADYFSRSIMQLGAAIPSFWIGMVFIVYFGVKLK
                     LFPVSGFIPFEVNFFRFIKSITLPSLVLAIGEIGTLLRIIRSSMLDSLKQDYMDMARV
                     KGLSSFKIYIKYALRGALTAPLTIIGMQFAKLAGGTVVVETIFALPGLGRLVLTAVEH
                     RDIVLLQGLVMFITSTVIIITLIVDISVMFFNPRVKSFQQGDL"
     gene            4912..>5491
                     /locus_tag="PLD67_03020"
     CDS             4912..>5491
                     /locus_tag="PLD67_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT21526.1"
                     /translation="MRKNISLILGIILVLIIIAVWVLSFVYLPYPPNEMNIPNKFLKP
                     GIDSEHILGTDNFGRDILSRIIYGSRNVLLVAISSVTLGALLGIFLGALAAMERFADT
                     IIMRIMDGLMAFPGILLAMMLVTVMERGIKGSIAAISIFMVPVFSRLVYSMILETEAL
                     LYIKAAKSYGSSRKQIFIRHYIPAMLPRLIFVF"
BASE COUNT         1750 a          933 c         1006 g         1802 t
ORIGIN      
        1 cattagcctt cggaatccac cccaatgacc tattattgtt attttcatta tctctcctta
       61 ttgatttatg aattgtgaat tgtataatcc ggcatacata ccctttttat ccaaaagctc
      121 ctgatgtctt cctttttcct gtatgccttg gtctgttaat actataattt catcggcatt
      181 tttaattgtt gataatctat gagctacaac tatcgtggtt cgtccctttg acaattcatc
      241 caaagctttt tgtatcaaaa actcggttgc attatcaaga gcggaggttg cctcatccaa
      301 tattattata ggcggatttt taagaaatac ccgagctatg gaaattcttt gtttttgtcc
      361 tcccgacaat tttactcctc tttcacccac ataagtgtca tacccttcag gcaaagacat
      421 tataaaatca tggatgttgg catttttggc tgctttgtat acttcttcct tggtggcatc
      481 gagtttaccg cattgaatat tttcaagaat agttccggta aacaagaata catcctgctg
      541 aacaattcct atgttctttc tgagagattt tcgtgtaaac tttcttatat caatattatc
      601 aatgctgata cttccctctt ctatctcata aaaacgcggt attaaatgac aaagggttgt
      661 cttgcctcct cccgatgggc caacaatggc aaccgtcttg cctgcttcaa tatcaaatgt
      721 tatatccttt aatatttctt tattatcatc ataagcgaaa gagacgtcat caaaacttat
      781 ttcacccttt acatcataaa tgtctgcagc gccagggtca tctttttcga catccttgtc
      841 cattatttcg ataaatcgtt tgaatcctga gctgcccgat tgatactgct caacaaagtt
      901 gactaatttt cgtatagggt ttaaaaagtt ccccgtatac aataagaacg ctgcaaaatc
      961 tccaaagttg attatcccta taaaaaagaa tatggctccc gagcttaaaa caacaatatt
     1021 taaaatgtca attataaatg aagagccgac atgaaattct gccattacct tgtaggcttt
     1081 tcttcttgct tcctgaaatc tgctgttgtt tttttcgaat ttttcaattt cattttcctt
     1141 attgctgaat gctttggaaa ctcttattcc tgatatgctg ttttcaatgg ttgcattaac
     1201 ttcacctatc gtaacccttg tttccataaa ggcatcttcc attttgagtc taagcttcat
     1261 tgcaacaatt aacaaaagag gaactgctga aaatacaatc aatgaaagag gcaggttgat
     1321 tgtgcttaac ataataaaag agcctataag catcaccaaa gatatgaata aatcctcagg
     1381 tccgtggtga gcaagttctg aaatatccat taaatcattt attattcggg atgataatgt
     1441 tcccgttttg ttttcgtcaa aaaatacaaa gggcaggtct tgcagatgct caaatatgtc
     1501 ttttctcata tgagcctgca tttttactcc cataatatga ccgtaatatg taatataata
     1561 attaagtccg aatttaagaa tgtatatgaa tcctaataca acaagccatt ttatcataag
     1621 ctgaaattct tgattcggta catagatgtt tattatgttt cttgttatca tgggataaaa
     1681 caaatcacat actgctactg caaatgcaca aatcatgtcg gtaataaaaa ttattttcac
     1741 cggtttataa taagctataa atcttttaag cattttttac tcctgtcgat tttcaaattt
     1801 tgaatatatt aatgctgcag ccgtaaataa aattatagaa gcaaaatcca ataatacgaa
     1861 aagtatgaat cctaatctta taaaataact tataatggca attaaaatta aagaaattat
     1921 cattgcagct tttgcatttt tgtgagcact atatttgaaa attctttcaa atattttatg
     1981 gacacttgca ttgtgcataa taattcccaa gattgctgcc gttgctgcat atcccggcct
     2041 tttagacatt aatgagcaac cttgcgaaaa tacaaatatt ccaattccca atataaagtg
     2101 gtaaaaaaac ctgtgttcac gcatttgctt tcgctgttgt tgacgtaatt cgtacataat
     2161 atcacctcta tttgtttaga taataatatc atttattgct aattttttca acgaacaatt
     2221 tatgcatatc ctctattgac tttataaaga aaatatgtta aacctagaaa aggaagaggg
     2281 acataccggt ccctgccaaa taataatatg ttttcaagga tttattacag atacgtatga
     2341 tttgattata aagatttgaa aacgggtata ttattattgg cattcagtaa aagatattct
     2401 aaatgaaatt ttattaactg aaaggaaaag aacaatgaaa aaaattatag caatgctttt
     2461 aattgcaatg ctgatttcag catgcacagc tcaagaaaac acacctgaaa aagcagatgt
     2521 taagactgaa cttaccatgg gatatactac ggagcccgaa ggtcttgacc ctcacagaac
     2581 tgctgcagct tccacattta ctgtaaccaa caatatatat gacactttgg taggcgtaac
     2641 gccgcaatgg gaaattgtgc ccagactagc aaaggaatgg gttatttctg atgacggaat
     2701 ggaaattaca ttctcattga gggatgacgt ttattttcat aacggaagac agatgactgc
     2761 caaagatgtg gagtattcat ttaccaggct taaggatgct gaaagcccca gggcaagaga
     2821 ttatgaaaat atagtaaaaa ttgaagttct aaacgactat gaaataaagt ttataactgc
     2881 agaattggat gttgagcttt taaagagctt tgtatatcca tgggcagccg ttgttccgga
     2941 agaagaagtt gcaaacttaa aaaccgaacc tgtcggaacg ggagctttta ctctggaaga
     3001 atgggtacct cagcaacatt tgattttaaa aagaaatgaa aattattacg gtgatgaaat
     3061 aaaattagaa accgttaaga tggttttaat acctgatgct accggcatga tggcgggatt
     3121 tcaggtagga gatcttgaca ttattccatt aacgggagat caggtaacaa tggttgaagg
     3181 aaatgaaaat tataaaataa tttctgagcc tatgaatgcc gttcaaataa tgtctcttaa
     3241 tacaaaaaat gaatatcttt caaatgaaaa agtcagacag gcaatggcaa tggctataaa
     3301 taaagatgag gttattgaag cttccatgtt tggatatggt gctaaaatcg gctcacacct
     3361 gcctcctaca tcacctgatt attatgacac aaataatata atagagtata atcccgaaaa
     3421 ggctaaagag cttttgaagg aagcaggata tgaaaacgga tttgaggttg atatgtcact
     3481 tcccaagaat tatcagcttc atgttgatgc ggggcaggta attgcagacc aattgggaag
     3541 gataggaata aaagtgaata ttcagcttgt tgagtgggga acatggttaa gcgaagtata
     3601 tggagctaag aactttgata ccacggttgt cgggcatact ggaagacttg attcatatgc
     3661 atttctttcg agatataagt cagacagcaa cgattatata agcttgacta caggagaagt
     3721 tgatgcgctt ttggaagaag ctcttaaaga attggatgaa aataaaagaa aagaaattta
     3781 caaagatata caggtaattt tagcaaataa gcttcctgca atttatttgc aaactcctca
     3841 cacaatgttg gcacttcaaa aaaatgttga aggaatgaag atatttccta tagactttta
     3901 tgattataag gatgtgtatt ttacagaata gctaaattgc cctctgaggt gtcttatgtt
     3961 taattacgtt ttagatagac ttaaaagctc aatagttgtt ttgtttgtag tgtcgattat
     4021 tacgtttttt gtgttaatga taatacccgg aaaccctgca cagttaatat tgggagcgga
     4081 tgcaactcct gaagcgattc aggagctgca tgttgctatg ggacttgaca gaccgctcta
     4141 tcagcaatat ttcagctggc ttttggattt gctaaaactt gacatgggta aatcctacgt
     4201 ctatggtgaa tcggttactg tcttaatagc aaaaagtata cccgtaactt tttcattatc
     4261 aatttttgct atgattatgg cagcatttat agcattcctt tttggaatga tttcggcaat
     4321 caaaaaggac agtattgcag attatttttc aagaagcatt atgcagcttg gagcagccat
     4381 tccttccttc tggataggaa tggtttttat agtttacttt ggtgtgaagc taaaactatt
     4441 tcctgtttca ggctttattc catttgaagt caactttttt agatttataa aaagcataac
     4501 tttaccctct cttgttctgg caatcggaga aataggaaca cttttaagaa ttataagaag
     4561 ctccatgctc gattctctta aacaggatta tatggacatg gcaagagtaa aagggcttag
     4621 cagctttaaa atatatataa aatatgcctt aaggggtgca ttgactgccc cattgacaat
     4681 aataggaatg cagtttgcca agcttgcggg aggaacggtt gtggttgaaa caatatttgc
     4741 ccttccggga cttggaagac tggttcttac tgccgtagag cacagggata ttgtgctttt
     4801 gcagggtctt gttatgttta tcacatcaac ggtaataatt atcactttaa ttgttgatat
     4861 atcggtaatg ttttttaatc caagggttaa gtcctttcaa cagggggacc tatgagaaaa
     4921 aatataagct taattttagg aattatatta gtgttgatta ttatagctgt ttgggtacta
     4981 tcatttgttt atttacccta tcctccaaat gaaatgaaca ttccgaacaa gtttttgaag
     5041 ccgggcattg attcagagca tattctgggt accgataatt tcggcaggga catactaagc
     5101 cgtataatat acggttcaag aaatgtcttg ttagttgcaa tatcatctgt aacactgggt
     5161 gccttgctcg gaatattttt gggagctctt gctgccatgg agcgctttgc agatacaata
     5221 attatgcgta ttatggacgg gcttatggct tttccgggaa tacttctcgc aatgatgctg
     5281 gtgactgtta tggaaagggg aattaaaggc tcaatagcag cgataagtat ttttatggta
     5341 cctgtatttt caaggcttgt gtattctatg atattagaaa ctgaagcact tttgtatata
     5401 aaagctgcaa aaagctacgg gagttcaaga aaacaaatat tcatcagaca ctacatacct
     5461 gcaatgcttc caagattaat atttgttttc t
//
LOCUS       DAOSOW010000076         5361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_15397_length_5361_cov_3.715228, whole genome shotgun sequence.
ACCESSION   DAOSOW010000076 DAOSOW010000000
VERSION     DAOSOW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15397_length_5361_cov_3.715228"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PLD67_03025"
     CDS             complement(<1..553)
                     /locus_tag="PLD67_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT21527.1"
                     /translation="MFKAREYYSDILDEIKKSGLWKEERIITTPQRSIISTTKAGGVL
                     NMCANNYLGLADNEEIIEAAKNAYDKWGYGLSSVRFICGTQEIHKELEKKISEFLKTE
                     DTILYSSCFDANGGLFETITTENDAIISDELNHASIIDGVRLSKAKRYRYNNNDMESL
                     EEQLKQADKDGANIKLIATDGVFS"
     gene            complement(717..1406)
                     /locus_tag="PLD67_03030"
     CDS             complement(717..1406)
                     /locus_tag="PLD67_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT21528.1"
                     /translation="MRIFAIADLHFDFKKEKPMDVFGDNWENHEEKIIENWKNTVDEE
                     DLVLIPGDISWALKLNDALEDLIRIENLPGMKIIGKGNHDYWWSTISQLDRLNLKTIK
                     FLKNNFHKYDDVVICGTRGWDTMEEYCAEVDNEKIFLRELNRFKISLQAAKKVSGKKI
                     IMLHYPPFNNNGLPNEFFSLMKDYNADICVYGHLHGEEGHRNVIEGLIDNIMVHCVSS
                     DYTGFKLKRIL"
     gene            complement(1415..4090)
                     /locus_tag="PLD67_03035"
     CDS             complement(1415..4090)
                     /locus_tag="PLD67_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT21529.1"
                     /translation="MYINRLNLRAFGKFINRKIHFSNKFNIIFGENETGKSTIHNFIE
                     LVMYGFDEDTSRYNKYKPWNSPVYKGSIEIADEEKKHIISRDFLLDTIQVFKKTNETK
                     ENEYSVEGIGVPGEHFFNINRVSFNNTVSISQLGNKTEKELADELKNKITNLSNTKDE
                     DISIDRIMQSLYRIKEEAGSENNDKTLLGQYSLRLEELRNAKESSMNSNRQVMFLAME
                     KKKLQSKIHELNMKIEGKQKEISDYELFLEKQKFLRAQPVKKEIDNINDELKLIEDDV
                     TNVSAEDYKEASETESSLASMKNDKQRLIEEKEECISELELIESDLSNYIPEEFDIDK
                     LNIDYDIYKANNEKIDTLKMKMKTGRESINSINIDEINNFINDYREVEELNHKIEINN
                     VLTDSKNYELMKKFGKKQRGISLFTGLLGTAFISAAAVSCYFAYFYNNFNYYYGGIGF
                     LIGILFYAFSWRKRNIAINAKKEIESMECEFADYTLSNNELNKQKDEILKRNNCQDFN
                     SMADAFQEKIKEKSIYEEKIKLLNYDDITFNEISAENKKIEEKLNNNLKTLNLSLSEN
                     TIIEVNEAYKRKDSVKLQTEELNKKIEKINRDITRLDREINFEQRRLEMILQANGADD
                     VEVFKKAVELNEKYKELLNRREYYEKILENIIGSTDYYELKKRTLNVSDEVKEIDKQD
                     IQLSIFKLNEEKTKLLQNINDIHHEIRDIEDNTRSLAEIEEEISFYEEKTAFFKNKIK
                     VAEIAAEKIRKISDSIKGDFMPLLRSSISDNFSYLTGGKYKSVDIDENMNITVMSEEE
                     KDRKIELENLSGGTLDQLYFSLRIALSNILSGNQNIPLILDDSFIQYDSKRLRKSLEM
                     LSRESERRQVILFTCQEREAELSKQMNIKFNYFKL"
     gene            complement(4092..5186)
                     /locus_tag="PLD67_03040"
     CDS             complement(4092..5186)
                     /locus_tag="PLD67_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT21530.1"
                     /translation="MKLKFIHTSDLHLGKKFDIKNFSLKERKKRRQELWDSFDEIIRT
                     AKIEEVQYLFIAGDLTDGEYISYKSLNKISSKFSSIPDTNVIITCGQSDPYNVNNMYE
                     YIEWPDNVYIIKNTETVEKLNFPENNLCIYSISWDNHAENKRTQPIYDISVDENKLNI
                     LMLHCDIDSSDRLPVNIDIIKNKFDYCPLGGRHNFRQITDNVVYCGTPEPLSFDEEGE
                     HGIIRGTLEKNNTQYSFLPIAKRKFINRQIELNISYSFNKILDLIKFSGDTFSNIKDY
                     VRIELTGEVNADISMDEIENEAKQFFYYIEFEDNYIYDTENRNYEGNEFNIIESYKLQ
                     FDKNDDNDDKLQQQAFKLGLEVLRKEKVVK"
BASE COUNT         1448 a         1028 c          708 g         2177 t
ORIGIN      
        1 tagagaatac gccgtctgtt gctatcaatt taatattggc tccatccttg tcagcctgtt
       61 tcagctgttc ttccaatgat tccatatcat tgttattata tctgtatctt ttagctttcg
      121 ataatcttac tccgtcaata atgcttgcat ggttcagctc gtcgcttatt atggcatcat
      181 tttcggtggt tattgtttca aacaagccgc cgtttgcatc aaaacatgat gaatacaaaa
      241 tggtatcttc ggttttaaga aattcgctta tctttttttc aagctcttta tgtatttctt
      301 gggtgccgca aatgaatcta actgaagaaa gtccgtaacc ccatttgtca tatgcattct
      361 tcgctgcctc gattatttcc tcattgtccg ccaatcctaa atagttattg gcacacatat
      421 tcagtactcc gcctgctttc gttgtgctga taatacttct ttgaggtgtg gttataattc
      481 tttcttcctt ccaaagaccg ctttttttaa tttcgtccaa tatgtcggaa tagtattccc
      541 ttgctttaaa cattctgttt cctcccatct ttattctttg ttcagtacaa ttttccgctt
      601 caatcttatt actttatcca aataatactc ttgttatgtt tttatgtcaa gagccgatta
      661 gatagtttgt ccatctattg tatacaatat ttgatgacag cacccccgtc cccttgtcat
      721 aatattcttt tcagcttaaa tccggtgtag tcgcttgaaa cacaatggac cataatattg
      781 tctatcaaac cctctattac atttctatgc ccttcttccc cgtgaagatg gccgtaaacg
      841 caaatatctg cattatagtc tttcatcagt gaaaaaaatt cgttaggaag tccgttattg
      901 ttaaagggag gataatgaag cataattatt tttttgccgc tgaccttttt tgctgcttga
      961 agggaaattt taaatctgtt caattctctt aaaaatattt tttcattgtc aacttccgca
     1021 caatactctt ccattgtatc ccaacctctg gtaccgcaaa taacaacatc atcgtattta
     1081 tgaaaattgt tttttaaaaa ctttatagtt ttcaaattca gtctgtcaag ttgggagata
     1141 gttgaccacc aataatcatg gtttcccttg cctataattt tcatgccggg taaattctcg
     1201 attctgatta aatcctctaa ggcatcattt aactttaacg cccatgatat atcaccggga
     1261 attagaacca aatcttcttc atctacggta tttttccagt tttcaattat cttttcctca
     1321 tggttttccc aattgtcgcc aaatacatcc attggctttt cttttttaaa gtcaaaatgt
     1381 aaatcagcga tagcaaaaat tctcataatt atccttatag tttaaaatag ttgaatttta
     1441 tattcatttg ctttgaaagc tctgcttctc tttcctggca tgtaaagaga attacctgtc
     1501 ttctctcact ttctcttgaa agcatttcta atgactttct aagccttttt gaatcatatt
     1561 gaataaagct gtcatccaaa atcagcggga tattctggtt gccgcttaaa atattgctta
     1621 gggctatacg cagtgaaaaa taaagctggt ccaaggtacc tccgcttaaa ttttcaagct
     1681 ctattttcct gtccttttct tcctcagaca taaccgtaat attcatattt tcatcaatgt
     1741 caacactttt atatttcccg cctgtcaagt atgaaaaatt atcgcttatt gagcttctaa
     1801 gaagaggcat aaaatcacct tttattgaat ccgatatctt ccttattttt tctgctgcaa
     1861 tttcagctac tttgatttta tttttaaaga aagcggtttt ttcttcataa aagctgattt
     1921 cctcttcaat ttccgctaaa cttcttgtgt tgtcttcaat atctcgtatt tcatgatgta
     1981 tatcgtttat attttgtaac agctttgttt tttcttcatt taacttgaaa atactaagtt
     2041 gaatatcttg cttgtcaatt tctttgactt catcggaaac attcaaagtc ctttttttaa
     2101 gctcgtaata gtcggtgctg ccaattatat tttccaaaat tttctcgtaa tattccctgc
     2161 ggtttaaaag ctccttgtat ttttcattca gctcaactgc tttcttaaat acttctacat
     2221 catccgcacc gtttgcttga agtatcattt ccaagcgtct ttgctcaaag tttatttccc
     2281 tatcgagtct tgttatatca cggttgattt tttcaatttt tttgtttaat tcttctgttt
     2341 ggagctttac tgagtcttta cgcttataag cttcatttac ttcaattata gtattttcgg
     2401 acaaggataa gtttaaagtc tttaaattat tattgagctt ttcctctatt tttttgtttt
     2461 ctgctgaaat ttcattgaat gtaatatcat cataattaag aagcttaatt ttttcttcgt
     2521 aaatgctttt ttccttgatt ttctcttgaa atgcatcagc catactatta aagtcttggc
     2581 aattgtttct ttttaatatt tcatcttttt gtttatttaa ttcattgttt gataatgtat
     2641 aatcagcaaa ctcgcattcc atactttcaa tttctttttt tgcgtttatt gcaatatttc
     2701 tttttctcca tgaaaaagca tagaataaga tccctatcaa aaaacctatt ccgccgtaat
     2761 aatagttgaa attattgtaa aagtaagcaa agtagcatga tactgccgca gccgatatga
     2821 atgccgttcc caaaagtccc gtaaaaaggc ttatccctct ttgttttttg ccgaatttct
     2881 tcatcaattc ataatttttg ctatccgtta agacattatt aatttctatt ttatgattga
     2941 gctcttcaac ttccctgtag tcattaataa aattgttaat ttcgtctata ttaattgaat
     3001 ttatgctctc cctgccagtt ttcatcttca ttttaagagt gtctatcttt tcgttatttg
     3061 ccttatatat gtcataatcg atatttaact tatctatatc aaactcttcg ggaatataat
     3121 tagacaaatc agattctatt aattcaagct ctgaaatgca ttcttctttt tcttcaatca
     3181 atctttgctt atcatttttc attgaagcca gactgctttc tgtttcagac gcttctttgt
     3241 aatcctccgc tgatacattc gtaacatcat cttcaattaa tttaagttca tcgttgatat
     3301 tgtctatttc cttctttaca ggctgagctc tcaggaattt ttgcttctcc aaaaaaagtt
     3361 cataatctga aatttccttt tgttttcctt caattttcat attcagctca tgaattttac
     3421 tttgaagctt tttcttttcc attgccaaaa acattacctg tctgtttgaa ttcattgaac
     3481 tttctttggc atttctgagc tcttccaacc taagcgaata ctgacccagc agtgttttat
     3541 cattattttc gcttcccgct tcttctttga ttctatacag gctttgcata attctgtcga
     3601 ttgaaatatc ttcatctttt gtattgctta aatttgttat tttatttttc agctcatcag
     3661 ccaattcctt ttctgtttta tttccaagct gactgatact tactgtattg ttaaatgata
     3721 ctctgtttat attaaagaaa tgctcacccg gaacaccgat accctcaacc gaatattcat
     3781 tttcttttgt ttcatttgtt tttttgaaaa cctgtatagt atctaataga aaatctcttg
     3841 aaattatatg ttttttctct tcatccgcta tttcaatgct tcccttgtac acggggctgt
     3901 tccaaggttt atatttatta taccgagatg tatcttcatc aaatccatac ataaccaact
     3961 caataaaatt atgtatggta gattttcctg tttcattttc accgaagata atattaaatt
     4021 tattgctaaa atgtattttc ctgttgataa actttccaaa ggctcttaaa ttcagtctgt
     4081 ttatgtacat tttattttac caccttttct tttctgagaa cttcaagtcc cagtttaaat
     4141 gcctgctgct gtaatttatc gtcattatcg tcatttttgt caaattgaag tttatagctt
     4201 tcaataatat tgaattcatt gccttcataa tttctgtttt ctgtatcata tatataattg
     4261 tcttcaaatt ctatataata aaaaaactgt tttgcctcat tttcaatttc atccattgaa
     4321 atatcggcat ttacttcacc cgtaagctct atgcgcacat aatccttaat gttggaaaaa
     4381 gtatcgccgg aaaatttaat caaatccaat attttattaa agctgtatga aatatttaat
     4441 tcaatttgtc tatttataaa ttttcttttt gcaatcggta aaaatgagta ttgtgtatta
     4501 tttttttcca aggttcccct tataatccca tgttcgcctt cctcatcaaa agacaaaggc
     4561 tcaggagtac cgcaatacac aacattgtcc gttatttgac ggaagttatg tcttcctcca
     4621 agaggacaat aatcaaactt attctttatt atgtctatat taaccggcaa tctgtcgcta
     4681 gaatcgatat cgcaatgaag catgagaatg ttgagcttgt tttcatcaac tgatatgtcg
     4741 taaatgggct gggttctttt attttctgca tggttgtccc aacttattga atagatgcac
     4801 aaattgtttt cgggaaaatt cagtttctca actgtttcgg tatttttaat tatgtatacg
     4861 ttatccggcc attctatata ttcatacata ttgttaacat tataagggtc gctttgaccg
     4921 caggtaatta ttacatttgt gtccggtatg gagctgaatt tcgaagaaat tttatttaaa
     4981 cttttatagc ttatatattc tccgtccgtt aaatcccctg caataaacaa atattgtact
     5041 tcctcaattt tggctgtcct tataatttca tcaaaagaat cccataattc ctgtcttctt
     5101 tttttgcgct cttttaaact gaaattttta atatcgaatt ttttccctaa atgcaggtca
     5161 gatgtatgta taaatttaag tttcatagca gctccttcga tttatgtgaa tatataatat
     5221 tttaccacag atagtataca tcttcaatat gatagataaa tttaatcatt gattagtctg
     5281 ccaattcaca gagtttatcc atgtccaata ttttaacgga tgagcggtaa acttcaataa
     5341 ttttttcctt ttgcatattg g
//
LOCUS       DAOSOW010000077         5357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_15411_length_5357_cov_3.700490, whole genome shotgun sequence.
ACCESSION   DAOSOW010000077 DAOSOW010000000
VERSION     DAOSOW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15411_length_5357_cov_3.700490"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLD67_03045"
     CDS             complement(<1..313)
                     /locus_tag="PLD67_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc chelation protein SecC"
                     /protein_id="HOT21531.1"
                     /translation="MKKELNKETARIILKALEDAKKHMQYLQERREEKLFSEIEIPCN
                     LNDVICRLTKDEMSHICKNYKLQGLSSLKKSELASELIKIIPHKFEGIIRILDQNTYD
                     LI"
     gene            complement(409..1137)
                     /locus_tag="PLD67_03050"
     CDS             complement(409..1137)
                     /locus_tag="PLD67_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOT21532.1"
                     /translation="MSETGIYHVIFRGVNRFNIFEEDKDYEKILEIISDLKEKISFDI
                     YAYVLMNNHGHLIIKELELGEISLIMKRILTRYAGWFNRKYDRSGILIANRFKSEPVE
                     TEDYLLTLARYIHQNPVKGGLTSKIDSYKWSSFKEYLGKSEICNTDFIMSIIDRDSFI
                     KFNFEINEEEYEISDKIQKFDDEFVKKRIKEILKGKEPTKLGEMPIDYRNRIIKQLIT
                     TEKFSIRQIERATGISRGVISRCK"
     gene            complement(1274..3007)
                     /locus_tag="PLD67_03055"
     CDS             complement(1274..3007)
                     /locus_tag="PLD67_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21533.1"
                     /translation="MNNTAQDDKRALNIIWNASDDYSIEPQFKAYDKNGEADIYWNYI
                     IGAVHKYYDYTLLRGFFNYLKTDREHEFYEKLFWIGLEKSAFDKGKNERPVIVSLRKN
                     YARKVLAGETEVFDDNLLNVLTNAHFKRALGKAPGITGNALNILNDLEFGDASTEEII
                     LKMNQIIKKYFRFNYGQYEVNAQINKKRVQRQIQYGEEERLSDSDLETMKELYLDSAE
                     TSREVFYEEKKGKIKKSAKKVKIEKKDDSERQYIRKYFGASIFPDKKTYLIERKLCIG
                     NHKNTHLHFTRGEFDDEFSNDINALYFNKALKEQREINLEHFNSNYIKYKNSILKLTN
                     KIRNTMLAYFESYTGKSEAGRLNAPKIWRNVYLNDNRVFTKVYNNDIGNLSVDILLDA
                     SASQHERQETIAAEAYIIAESFTQCQIPVRVYSFSSLRNYTVINLYRDYDETDKNNKI
                     FNYKTTGCNRDGLAIRTALYMMEGSNYLNKILIVLSDCKPNDTQCIPDTGIKQEQTKY
                     AGATGIIDTAFEVKRGINNGNSILCVFTGEDEDIPTAKKIYGHNFARIYYPERFAEIV
                     GVLMQNQLKNL"
     gene            complement(3010..3921)
                     /locus_tag="PLD67_03060"
     CDS             complement(3010..3921)
                     /locus_tag="PLD67_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOT21534.1"
                     /translation="MLEFLRKEGISENLINDVEKFRKFYKVESKMEYRIPTPKYNYYG
                     NDVWEEAITALLCGENLLLVGSKATGKNVLAENLSAAFGRPKWDISFHVNTDSTSLIG
                     TDTFEGGQVVFRNGPIYECAKWGGFGVLDEINMAKNDSVAVLHATLDYRRIIDVPGYD
                     KITIDDASRFIATMNYGYAGTRELNEALTSRFMVINMPNISSKNLRKLLQREYPNLRE
                     EYVEQFAGLFKDLQLKSENAEISTKSVDLRGLLASINLMDKGLSPFKALQMGITNKSF
                     DDFERTLVEDVIRLRIAENLGRNEIFR"
     gene            complement(3930..5287)
                     /gene="radA"
                     /locus_tag="PLD67_03065"
                     /pseudo
     CDS             complement(3930..5287)
                     /gene="radA"
                     /locus_tag="PLD67_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002598926.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
BASE COUNT         1406 a         1105 c          788 g         2058 t
ORIGIN      
        1 ttattaaatc ataagtgttt tgatctaata tacgtattat tccttcaaat ttatgaggta
       61 ttatttttat tagctcagat gctaattctg actttttcaa agagcttaac ccttgtaatt
      121 tatagttttt acaaatatgt gacatttcat ccttagttaa cctacaaatt acatcattta
      181 agttgcatgg aatttctatt tcactaaaaa gcttttcctc tcttctttct tgcagatatt
      241 gcatatgttt tttagcgtct tccaaagcct ttagtatgat tctagctgtt tctttattta
      301 attctttttt cattttcaac cccttgggga cgtaccttgg tgtcccactt ttttaagtat
      361 ctcatatttt ccttatttat tttaagtgtt tcatttatta taattgcgtt atttgcacct
      421 tgatattaca cctctggata ttcccgtagc cctctctatt tgtctgattg aaaacttttc
      481 agtggttatt aattgcttaa ttattctatt cctataatca attggcattt ctcctaactt
      541 agtaggttcc ttacctttta atatttcctt tattctcttt tttacaaatt catcatcaaa
      601 tttttgtatt ttatctgata tttcatattc ttcttcatta atttcaaaat taaattttat
      661 gaaactgtct ctatcaatta ttgacattat aaaatctgta ttgcatattt cactctttcc
      721 caaatattct ttgaagctgc tccacttata actgtctatt ttagatgtta gtccgccctt
      781 gacaggattt tggtgtatat accttgctaa ggttaataag taatcttctg tttcaacagg
      841 ctcgctttta aatctgtttg caattagtat tccacttcta tcatattttc gattaaacca
      901 gcctgcatat cttgtaagta ttcttttcat tatcaatgat atttcaccca attccaattc
      961 ctttattatt aggtgtccat gattgttcat taaaacatat gcataaatat caaagctaat
     1021 tttttctttt aaatcagaaa taatttctaa aattttctca taatctttat cttcctcgaa
     1081 aatattgaac ctgttaactc ctctaaaaat tacatgatat attccagtct cactcaattt
     1141 tctggcaacc cttggcatga agatcacctg cctttttgct tttaattata tgatattttc
     1201 catataatgt caaaatgtta aaatgatttc aaatatatgc agtgggacag caaggtacgt
     1261 ccccactgtc ccgctagagg ttttttagtt ggttttgcat tagaacaccg actatttctg
     1321 caaatctttc gggataatat atacgggcaa agttatgtcc gtatattttt tttgctgtcg
     1381 gtatatcctc atcttcgccg gtaaagacgc aaagaattga attgccgtta tttatgcctc
     1441 ttttcacttc aaaggcagta tctattatac cggtagcccc cgcatacttg gtctgctcct
     1501 gctttattcc cgtgtcggga atgcactggg tgtcattagg cttgcagtct gaaagaacaa
     1561 tgagaatttt atttaaataa ttgctgcctt ccatcatgta caatgccgtt cttattgcca
     1621 agccgtcacg gttgcagccc gttgttttat aattgaatat tttattgttt ttatctgttt
     1681 catcataatc acggtaaagg tttataacgg tgtaatttct taggcttgag aatgaatata
     1741 ctctgacggg aatttggcat tgagtaaagc tttcggcaat aatatacgct tccgctgcta
     1801 ttgtttcctg tctttcatgc tgcgatgcgg atgcatccag caaaatatct accgataaat
     1861 ttccgatatc gttgttatat actttcgtga aaactctgtt atcatttaaa tagacatttc
     1921 tccaaatttt tggtgcatta agtcttcctg cttcggattt tccggtatat gattcaaaat
     1981 atgcaagcat agtgtttctg attttatttg taagctttaa aatactgttt ttgtatttta
     2041 tataattgga attgaaatgc tctaaattaa tttccctttg ctctttcaat gctttattga
     2101 aatacaatgc attaatatca ttgctgaatt catcatcaaa ttctcctctg gtgaaatgaa
     2161 gatgagtatt tttatggtta ccgatacaaa gttttcgctc tattaaatat gtttttttat
     2221 cgggaaaaat tgaagcaccg aaatatttac gaatatactg tctttccgaa tcatctttct
     2281 tttcaatttt aactttctta gctgattttt taatttttcc ctttttttct tcatagaaaa
     2341 cttctcttga cgtttcagct gaatccaaat acagctcttt catagtttcc aagtcggaat
     2401 cgctaagtct ttcttcttcg ccgtattgga tttgtctttg cactcttttt ttgtttattt
     2461 gggcgtttac ttcatactga ccgtaattga acctgaaata tttcttgatt atttggttca
     2521 tttttaaaat tatttcttct gttgaagcat caccgaattc aaggtcgttt aaaatattta
     2581 aagcattgcc tgttattccc ggagctttac ctaatgctct tttaaagtga gcatttgtca
     2641 gtacatttaa taaattatca tcaaaaactt cagtttcacc tgctaatact tttcttgcat
     2701 aattttttct aaggcttact attacaggcc tttcgttttt tcctttatca aatgcactct
     2761 tttccaagcc tatccagaaa agtttttcat aaaattcatg ctctctgtct gttttaagat
     2821 aattaaaaaa gcctctcagc aaagtgtagt catagtattt gtgaacagcc cctattatat
     2881 aattccaata gatatccgct tcaccgtttt tatcataagc cttgaattga ggctcgatgg
     2941 aatagtcgtc cgatgcattc caaataatat ttagagctct tttatcgtct tgtgctgtgt
     3001 tgttcatgct cacctaaaaa tttcattcct tcctaaattt tcagctattc gaagacgtat
     3061 aacatcctct acaagcgttc gctcgaaatc atcaaatgat ttgtttgtaa ttcccatctg
     3121 caatgctttg aaaggagata gtcctttatc cattaaatta attgaagcta aaagtcccct
     3181 caaatcaacc gacttagttg aaatctcggc attttcgctt tttaactgca aatccttgaa
     3241 aagccctgca aactgctcaa catattcttc tcttaaattt ggatattctc tttgcaataa
     3301 ttttcttaaa ttctttgatg aaatattagg catatttatt accatgaaac gggatgtcaa
     3361 tgcttcgttt aactctcttg tgccggcata accgtaattc atggttgcaa taaaacggct
     3421 tgcgtcatca attgtaattt tgtcatatcc gggcacgtca attattcttc tatagtctaa
     3481 ggttgcatga agtactgcaa cggaatcatt tttggccatg tttatttcat ccaatacgcc
     3541 aaagccgccc cactttgcac attcgtagat gggaccgttt ctgaatacaa cctgaccgcc
     3601 ctcgaatgtg tcagttccga tcaatgatgt tgaatctgtg ttaacatgga acgatatatc
     3661 ccattttggt ctgccgaatg ctgcagataa gttttccgcc agcacatttt tgcctgtagc
     3721 tttggagccc accaacaaca ggttttcccc gcaaagaaga gcagtaatcg cttcttccca
     3781 aacatcattg ccgtaataat tgtactttgg cgtaggaatt ctgtattcca tctttgattc
     3841 taccttataa aactttctga acttctcaac gtcatttatc aggttttcgc ttattccttc
     3901 ttttctcaaa aattctaaca tttttcctcc taaaatataa tgtccataac ttgtcttatg
     3961 ttgtttaccg ggattatttc catgttttta acttcttttg aaatttctgc attggcttct
     4021 gctatgaccg ctcttttaaa tcccatcttt tttgcttcca aaagtctttt ttcaattgaa
     4081 ttaactcctc ttatttctcc ggttagccct acctcgccta taagaacggt cttaaagtcg
     4141 acgggtattt ctttgaagct tgatgccaaa gcgcatagta ttgccaaatc gcatgcaggc
     4201 tctttgattt gcagccctcc ggttacatta ataaatatat cggagctttg aattgacaga
     4261 cctgctttct tttccaatac agcaatcagc atggatgccc tgtttttatc aattcccgtg
     4321 gttaccctgt tggcaaatcc aacaggagaa tatgttacca aggcctgaat ttctatcaga
     4381 acgggtctcg taccttccat tgctgcagta actacggtgc cgtaagcatc aagaggtctt
     4441 cccttcaaaa atacttccga aggattttca acctctataa gtcctctgtc ccgcatttca
     4501 aaaataccta tttcattggt tgagccaaat cgatttttaa cagctcttaa tattctatat
     4561 gaaaaatgcc tctctccttc aaagtataaa acagtatcaa ccatgtgctc aagtactctt
     4621 ggtcctgcaa ttgcacctga ttttgtaacg tgtcccacta tgaaggaagc catatttctt
     4681 gttttcgtaa ttctcattac cattgaagtc acttccctga cctgggtaac gctgcccggg
     4741 gcactgggca catcgggtga atatatagtc tgaattgaat caagtacaag caaatccggc
     4801 tcttctttat ctacgtgctc gattattatg tcaatattcg tttcagcaag aacatacagc
     4861 tcaccgtctc ctatccccaa cctatcggct cttattttta tctgctctcc cgattcttct
     4921 ccggatacat aaagtacttt taatttttgc tttgcagcgt ggtctgcaac ctgcagaaga
     4981 agtgttgact tgccgatacc gggatctcct ccaactagta tcagggagct ttttattatt
     5041 cctccgccca ataccctgtc catttcaacg cttcctgttg agattctttc ttttttggtg
     5101 gatgtgatct tgtttatttt tcaattatac ccttgctgat acctctttga cttttggagt
     5161 cacttttttt agtttcaaat tcctccacaa aggtattcca ttcttcgcag gagggacatt
     5221 tgccaagcca tttggccgtt tcataaccgc attcctggca aacaaatttg gattttatct
     5281 tagccatgat tcacctgctt tcactgcttt catacgctaa ttaactataa cgcaaacctg
     5341 tataatatgt caatagt
//
LOCUS       DAOSOW010000078         5352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_15430_length_5352_cov_3.998301, whole genome shotgun sequence.
ACCESSION   DAOSOW010000078 DAOSOW010000000
VERSION     DAOSOW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15430_length_5352_cov_3.998301"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..1011
                     /gene="spo0A"
                     /locus_tag="PLD67_03070"
     CDS             217..1011
                     /gene="spo0A"
                     /locus_tag="PLD67_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HOT21535.1"
                     /translation="MQKKVKVVIADDNKEFRLILKDFLLSKKVFDVVDMAEDGIKTLE
                     SVEKHEPDILILDIIMPQLDGLGVLERLHKKELKKFPKVIVLSAVGHDKVTQRAINMG
                     VDYYIVKPFNFESFADRLLQISELETSKVQNKNKEIVYNEKINSELSLEVKITEILHE
                     VGVPAHIKGYQYLRTSIIDVVKNVELLGAITKELYPMIAAKYSTTPSRVERAIRHAIE
                     VAWTRGKIETINNIFGYTIHNNKGKPTNSEFVAMIADKLRLEQRVS"
     gene            1122..1646
                     /locus_tag="PLD67_03075"
     CDS             1122..1646
                     /locus_tag="PLD67_03075"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOT21536.1"
                     /translation="MVNKEITVKSEKIFEGKIVNLRVDTVELQNQKYAKREIVEHKGA
                     ACILAVNNDKVIFVRQYRKAIEENILELPAGILNIAEEPPEGALRELKEETGYKAGKI
                     ELIYEFYTSPGFSNEKVYLFKASELVYEGTDLDEDECLETIEVDRDEAKKMLETGKIN
                     DSKTIIGLLHWLNS"
     gene            1777..2358
                     /locus_tag="PLD67_03080"
     CDS             1777..2358
                     /locus_tag="PLD67_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21537.1"
                     /translation="MNNTLKKLVKSENKKFIALILIFIGALILSALIYSLTGKGSLSE
                     INYESISKMNFLQIFGSSIKRNIIYFLAVIFLTYFGQGYLTMILFGFISVYYGLSVIY
                     IIRTVGMDLKYFMITFTDYFIFFPILLYFTFISSSIAKYTKKAKNIETISRKFDIIIS
                     GYLRISLFYLLIVTAYSFVYSLYVLILSRLMVR"
     gene            2358..3224
                     /locus_tag="PLD67_03085"
     CDS             2358..3224
                     /locus_tag="PLD67_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOT21538.1"
                     /translation="MEITMYKEYLYQRNLSENTIDSYIYDVESFRNYLREDYELELNE
                     TKKAHILTYLVTLQKNGKSSSTLSRTISALKNFFDFLKNEKLIKDNPAVSIHSPKQIK
                     KKPSILTEEEVAQLMLLPDKNTFKGIRDLAMLELMYSSGIKVSELISIKLSQINLSAG
                     IISIEGEKERIIPLSKYAISAIENYLNNFRDKNTDENDFLFINITGEPLTRQGIWKSL
                     KSYKKKMNLNKELSPQILRNSFAVHMLSHGADLGTIQELMGLNSLGAAQNYINNLEFK
                     SLEVFNKTFPRG"
     gene            3338..4516
                     /locus_tag="PLD67_03090"
     CDS             3338..4516
                     /locus_tag="PLD67_03090"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HOT21539.1"
                     /translation="MKRTVLIVLDSVGAGELPDAAAYGDEGSNTLKNIYKNAKNFSLP
                     HLENLGILNIDGFEDLKSPEYFLGSVAKCSQKSKGKDTTTGHWEISGLILDKPFPTYP
                     DGFPEELISKFEKKIGRKVIGNYAASGTEIIKELGKEHVETGNIIVYTSADSVFQIAA
                     HEEVVPLDELYEICIIARKMLQGNNGVGRVIARPFIGEEGNYTRTGNRKDFSLEPYKD
                     TMLDFVKNSGREVYAIGKIEDIFANKGITYSNHTKNNEEGIEALIQAVKLDFEGLIFA
                     NLVDFDMIYGHRNNVQGYGDALMYFDGRLPEIMDNLKDDDILIITADHGCDPTTASTD
                     HSREYIPLIFYGKNIKENNNLGILETFASIGKTVLDIFDIKNDLKGTSVKNQIIKTGK
                     "
     gene            4646..>5352
                     /locus_tag="PLD67_03095"
     CDS             4646..>5352
                     /locus_tag="PLD67_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOT21540.1"
                     /translation="MKKILTIFFIFIILNMMVVTAYGIEPEDLQSRSAILINADDGRI
                     LFEKNANDKMQPASITKIMLMVLISERMADGQITLDQEMTISENAAGMGGSQIYLEAY
                     EVQTVENMLKAISMRSANDASVAMAEFMYGSEESCVKAMNEKAKELGMKDTVFTNVTG
                     LPDPEHLTTANDIAIMTRELLKYNYMDEYMLTWMDSVFVGKEKDSEQVLVNTNRLINN
                     YNGLLAGKTGYTTEAKY"
BASE COUNT         2012 a          753 c         1006 g         1581 t
ORIGIN      
        1 aaattcatta gtaaaatata acaaaaattt aaaaacaaat attttaattt atcgacaaaa
       61 ttttagtata aattaggaaa agctgtatat aatagacata tatacttttt atattacaaa
      121 attagaagac aaaaaatttt ttttataaaa aaaaagggtt ttttgattct tttgcgtatt
      181 atattagtat agaatatttt gaatggggga gtacttatgc aaaaaaaagt aaaagttgtt
      241 attgctgacg acaataaaga atttaggtta attctaaaag acttcttgct cagcaaaaaa
      301 gtcttcgatg tagtagacat ggcagaggat ggaataaaaa cattggaatc tgtcgaaaaa
      361 catgaacccg acattcttat attggacata ataatgcctc agcttgatgg gttgggggta
      421 cttgagagac ttcataaaaa agaattaaaa aagtttccta aagtaatagt tctatctgcc
      481 gtaggtcatg ataaggttac ccagagagct attaatatgg gtgttgacta ttatattgtt
      541 aagcctttca attttgaatc ttttgctgac agattacttc aaatatcaga attagaaact
      601 tctaaggtgc aaaataagaa taaggaaatc gtttataacg aaaaaattaa ctcggagctc
      661 agcttggaag tcaaaataac cgaaatttta catgaggtag gtgtacctgc acatattaaa
      721 ggttatcagt atttacgcac ttctataata gatgtagtaa agaatgttga attattggga
      781 gcaattacaa aggaacttta tcccatgata gcagctaaat attcaaccac gccaagcaga
      841 gttgaaagag ccatacgaca tgcaatagaa gttgcttgga ctcgggggaa aattgaaact
      901 atcaacaata ttttcggtta tactatacat aataataagg gtaaacctac caattcggaa
      961 tttgtagcaa tgattgcaga taaacttcgc ctggagcaaa gagtatcata gcaatagaaa
     1021 atggtgcacc tgtaaattaa cttgacaaaa atctgtattg ttattagaat aagtccaact
     1081 attaatcttg cttcagatgc aatgatttaa tataggaggc tatagtgaat aaggaaatta
     1141 ctgtaaaaag cgaaaaaata tttgaaggca aaatagttaa tttaagagta gatactgttg
     1201 aattgcaaaa ccaaaaatat gcaaaaagag aaattgttga gcataagggt gcagcctgta
     1261 tattagctgt taataatgat aaagtaattt ttgtgagaca atacagaaag gccattgagg
     1321 agaatatatt ggagctgccg gcagggattc ttaatattgc agaagagcca ccggaaggag
     1381 ctctgcgtga attaaaagaa gaaaccggtt ataaggcagg taaaatagag ctaatttatg
     1441 aattttatac ctcaccgggt ttttccaatg aaaaggtata tttgtttaag gcttcggagc
     1501 tcgtatatga aggtactgat ttagatgaag atgaatgcct tgaaacaatt gaagttgata
     1561 gagacgaagc aaaaaaaatg cttgaaaccg gtaaaataaa cgatagtaaa acaataatag
     1621 gacttctcca ttggctaaat tcttaaaata aaaacataaa aagtattaaa cctcttgtac
     1681 aagcatatat tatacaaagc ttaacaagag gttttattgt gcaaaaagga cgtcttatgt
     1741 cctgcttggc atacagacag taggtgggag gtttatatga acaatacact aaagaagctg
     1801 gtgaaatctg aaaacaaaaa gtttatagcc ttaatattaa tttttatcgg agctttgatt
     1861 ctatcggcat taatttattc attaacggga aaggggtctc taagtgagat aaactatgaa
     1921 agtataagta aaatgaattt tctgcaaatt ttcgggagtt caataaaaag aaatattatt
     1981 tattttttgg cggtcatctt tttaacatat tttggacaag gatatttaac aatgattttg
     2041 tttggtttta tttccgtata ttatggtttg tccgttattt atataatcag gactgttggt
     2101 atggatttaa aatattttat gatcaccttt actgattatt ttatattctt tcctattcta
     2161 ctttatttta cttttatttc aagttcaata gcaaaatata caaaaaaagc aaaaaatatc
     2221 gagactattt cccgaaaatt tgatataatt atatccgggt atcttagaat ttctttattt
     2281 tatttactga ttgtaacagc ttattcgttt gtatacagct tatatgtatt aatattaagc
     2341 agattgatgg tgagataatg gaaatcacaa tgtataaaga gtatttgtat cagagaaact
     2401 taagtgaaaa tacaatcgac tcctatattt acgatgtgga aagctttaga aattatcttc
     2461 gtgaagatta tgagcttgag ctaaatgaaa caaaaaaagc acatatttta acatatcttg
     2521 tgactctgca aaaaaacggc aagagttcat caacattatc gagaactatt tctgcattaa
     2581 aaaacttttt tgatttcttg aagaatgaaa aactgattaa ggacaaccct gctgtcagca
     2641 tacacagccc aaaacaaata aaaaagaaac cttcaatttt aactgaagaa gaagtagctc
     2701 agcttatgct tttgcctgat aaaaatactt tcaaaggaat cagagacttg gcaatgttgg
     2761 aactgatgta ttcatcaggt ataaaagtat ccgagctcat cagcataaaa ttatctcaaa
     2821 taaacctaag cgccggaata atttctattg aaggagaaaa agaaagaata attccattaa
     2881 gcaagtatgc aataagcgca attgaaaact atttaaataa ttttagggat aaaaacactg
     2941 atgaaaatga ctttttattt atcaatatta ccggtgagcc cttaacaagg cagggaatat
     3001 ggaaatctct aaaatcttat aagaaaaaaa tgaacttaaa taaggaatta tctcctcaaa
     3061 ttctaagaaa ttcattcgct gtacatatgt tgtctcacgg ggctgacctc ggaactatac
     3121 aggagttaat gggacttaat agtctgggtg ccgcacaaaa ttatataaat aatttagaat
     3181 tcaaatcctt ggaagttttt aataaaacat ttccacgcgg ttgatatttt catacataat
     3241 tgatgtttca gactgcacta ttaatggtat aataagacaa aaggaagaga gatataaatt
     3301 atctcttctt ttaatataaa taaaggagca accaatgatt aaaagaacag tattgatagt
     3361 cttagacagc gtgggagcag gtgaacttcc ggatgctgcc gcctatggcg atgaaggcag
     3421 caatacacta aaaaatatat acaagaatgc aaagaatttt tcattaccac atctggagaa
     3481 tttaggtatt ttaaatattg acggatttga agacttgaaa agcccggaat actttttagg
     3541 ctctgtggca aaatgcagcc aaaaatcaaa ggggaaggat acaacaacag gtcactggga
     3601 aatcagcggg ctgatactgg ataaaccatt ccctacctat ccggacgggt ttcctgaaga
     3661 gcttatttca aaatttgaga aaaaaatcgg gagaaaagtt ataggcaact atgctgcttc
     3721 aggaaccgaa ataatcaaag aattaggaaa agagcatgtg gaaaccggca atataatagt
     3781 ctacacatct gcagacagcg tatttcaaat agcagcccat gaagaagtgg ttcccttgga
     3841 cgagctctat gaaatttgca taattgccag aaaaatgttg caaggaaata atggagtagg
     3901 aagagtaatt gcaagacctt ttatagggga agaaggtaat tatacaagaa ccggcaatag
     3961 gaaagatttc tccttagagc cttacaaaga tacaatgctg gacttcgtaa aaaacagcgg
     4021 cagagaagtt tatgccattg ggaaaataga agatatattt gctaacaaag gtattacata
     4081 ttcaaatcat accaaaaaca atgaagaagg aattgaagca ttaattcaag ctgtaaaact
     4141 agatttcgaa ggcttgatat ttgcaaatct tgtagatttc gacatgattt acggtcacag
     4201 gaacaatgta cagggatatg gggatgccct tatgtatttt gacggcaggc taccggaaat
     4261 aatggataat ctcaaggatg acgatatttt gattattacg gctgaccatg gctgcgaccc
     4321 tacaactgcc agtacagacc attcaagaga atatataccc ctgattttct atggaaagaa
     4381 tataaaagaa aataacaatc ttggcatact tgaaactttt gcatccatag gcaagaccgt
     4441 attggatatt tttgatatta aaaatgacct taaagggaca agtgttaaaa accaaataat
     4501 caaaaccggg aaatagacta aattagacat tttttttaaa caactaactt agatatttca
     4561 ttatccgcag ataggaattt gctcatccaa ttaaatgtat gattcaacaa aatatggtta
     4621 ttctacataa aaaggaggta attttatgaa gaaaatttta accatatttt ttatatttat
     4681 tatcttaaat atgatggttg ttaccgcata tggaattgaa cccgaggact tgcaatcccg
     4741 ctctgcaatt ttgattaatg cagatgacgg aaggattttg tttgaaaaaa acgctaacga
     4801 taaaatgcag ccggcaagca ttactaaaat aatgcttatg gttttgattt cagaaagaat
     4861 ggctgatggt caaataacat tagaccagga gatgacaatt tcggaaaatg ctgcaggtat
     4921 gggaggaagt caaatatatc tcgaagcata tgaagttcaa acagtagaga atatgcttaa
     4981 agctatatcc atgcgctcag ccaacgatgc atcagttgca atggctgaat ttatgtacgg
     5041 ctcagaagaa agctgtgtta aggctatgaa tgaaaaagcc aaggaattgg gtatgaagga
     5101 taccgtattt acaaatgtta cgggccttcc ggaccccgag catcttacta ctgcaaatga
     5161 tattgctata atgacgcgtg agctgttaaa atataattat atggatgaat atatgcttac
     5221 ctggatggat tccgtatttg taggcaagga aaaggattca gagcaagtgt tggtaaacac
     5281 aaacagactg ataaataatt ataacggctt attggcaggg aagacaggtt atacaactga
     5341 agcaaaatat tg
//
LOCUS       DAOSOW010000079         5352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_15433_length_5352_cov_3.312441, whole genome shotgun sequence.
ACCESSION   DAOSOW010000079 DAOSOW010000000
VERSION     DAOSOW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5352
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15433_length_5352_cov_3.312441"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLD67_03100"
     CDS             <1..216
                     /locus_tag="PLD67_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HOT21541.1"
                     /translation="LNSIEKYIELNSREAIRTHEKYSGIKNEHFRFYKIASVILVLAQ
                     KTTAGVMVKFFYDMQRLKAIMMVKLQK"
     gene            complement(414..1226)
                     /locus_tag="PLD67_03105"
     CDS             complement(414..1226)
                     /locus_tag="PLD67_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="HOT21542.1"
                     /translation="MDFLSREGSPISAELWSKIDEQVISMAKKVLVGRRFLHIYGPLG
                     AGVQSINIDDSTQLSETEGDIVQIKGRKFEQIPLLNQDFSLLWRDLELSEKTGMPIDL
                     SSALQASAEISKKEDELIFNGNKELGYEGLLTAKGITKLELSNWEEGENPVKDITAGI
                     TNLFDKGLVGRKALIISSDLLIKLQRIQPGTGITEYERLSKLVDGNIYYTKVIGSKKA
                     VLVCAEPQYMDLVVGQDMATSYLETKDLNHYFRIIETILLRIKNKNAVVVFE"
     gene            complement(1236..1604)
                     /locus_tag="PLD67_03110"
     CDS             complement(1236..1604)
                     /locus_tag="PLD67_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein COQ7"
                     /protein_id="HOT21543.1"
                     /translation="MAGFGNPFGANVPRKLTKDELIQAVRLNIAGELEAIYVYDAHIQ
                     ATDDIVAKKIIGDIRDEEKAHVGELLTLLKYLDPKEAEFLNEGEEEVREMLEELNINM
                     ETAPSTDTNSSSTVGSLLKE"
     gene            1933..2889
                     /locus_tag="PLD67_03115"
     CDS             1933..2889
                     /locus_tag="PLD67_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOT21544.1"
                     /translation="MNVLNLDFRKQKMMRTVMLSLIPIIIFSVYNYGLRVLALLTVIT
                     ITGTAVEYLWEKHYNNKPSEAIFVTCILYTMTLPSSIPYWIAVIGIIFGVIFGKMSFG
                     GFGRNVFNPALVGRAFIYINFPNPMTIVWNEAASGFPGGFSTYLTAGIDAVSEATPMI
                     TYNYTGEIMDLNSLILGNIPGAIGESYKILIILAAIYLIYTKTASLEIMLGCLIGFFT
                     VGTFMYYIYDGINPIYGMMMGGFLFGTVFMATDPISAAKTKKGKWIFGIIIGVVTVLI
                     RALSLFNGGMMFAILMGNTFAPIIDHFIKEAENRKKSKAVAA"
     gene            2886..3479
                     /locus_tag="PLD67_03120"
     CDS             2886..3479
                     /locus_tag="PLD67_03120"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOT21545.1"
                     /translation="MKNSFIYPIIFMMAVTAVFISVLAGLNFVTADTISFNQESQLQQ
                     KILYIFDILPEGGTEKDIERVFNDSVIEKEWGELRGYALIQGGKETAYAIPVNGAGLW
                     GSIIGYIGLNADYTEIIGIEFVTQNETPGLGGRISEDSYKEQFRGIDISGKSENFIIS
                     SPEPGSNVDAIAGATQTSNAVVKLINDDIKIFLREVE"
     gene            3481..4104
                     /locus_tag="PLD67_03125"
     CDS             3481..4104
                     /locus_tag="PLD67_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOT21546.1"
                     /translation="MTKTEVKKIFKKGIYTDNPIFVQVIGICSALAVTNLMINSLIMG
                     IALALVTAFSSLTVSLLKDHTPKHIRMMIQVLIISAYVIMVDIYLKAYMPEISKALGP
                     YVGLIITNCIIMGRAEGFARSNPPIPSFFDGLASGIGYTFVLMSISFIREILGFGSIF
                     GISLIPETFTRWTLMVMPPSAFFILALIMWVFNNNRLKKQREGVERK"
     gene            4101..4691
                     /locus_tag="PLD67_03130"
     CDS             4101..4691
                     /locus_tag="PLD67_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOT21547.1"
                     /translation="MIQVHPFIIFIASIFTSNMIFSNFLGMCSFIAVSKEIPTALGLG
                     IAVTMVLSATTVLNYLVYWYIIMPLGLEYLMYILFILVIAAFVQLLEMTMDRYLPTLY
                     YALGIFLPLITVNCAILGVSLFMVTRQYNFFQTFGFAVGSGIGWALAITAMAGIRSKL
                     NENSIPEGLRGVPITLIITGIMALAFIGFSGMVQIQ"
     gene            4709..>5352
                     /locus_tag="PLD67_03135"
     CDS             4709..>5352
                     /locus_tag="PLD67_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002834993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOT21548.1"
                     /translation="MDTIIMTIVIFSGISGVLAAILTISDRTIGNYGEVTLTINEDKI
                     YTVKGGSSLLDTLRNEQIFIPSACGGKGTCGYCKVVVLEGGGPVLATEKPLLTKEELE
                     NNTRLSCQCKVKQNIKAKIPEELFNVKEYAVIVEKMEQLTPTIKKLRFNLGEEEISFK
                     PGQYVQLKAPAYEGNDEEVYRAYSLASSVNDKHAIELLIGFTGGIATTYVHQHL"
BASE COUNT         1619 a          878 c         1044 g         1811 t
ORIGIN      
        1 ttaaattcca tagaaaaata tattgaacta aacagcagag aagctataag aacgcatgaa
       61 aaatattccg gtattaaaaa tgaacacttt cgattttata aaatagcttc ggttattctg
      121 gttttagctc aaaaaacaac cgccggagta atggtgaagt ttttttatga catgcagcga
      181 ttaaaggcaa taatgatggt taaattgcag aaataaaaga gaaggtcctt aagctttgag
      241 attcttcggg ctaaagccct cagaatgaca aaaagtgaaa gatgaatcct tcgcttgcgc
      301 tcaggatgac atgcacaaaa gccaaagaaa ttttctttgg cttttgtgat tcttcgggct
      361 ggggccctca gaatgaaaaa aattatttat agcaatgata gaggtatcac atgttattca
      421 aatactacaa cggcattttt atttttaatt cttaagagaa ttgtttctat tattctgaaa
      481 tagtggttta aatcttttgt ttcaagataa gatgttgcca tgtcctgacc aacaaccaaa
      541 tccatatatt gcggctcggc acaaactagg actgcctttt tgcttcctat tacttttgtg
      601 taataaatat tgccgtctac taatttgctt agtctttcgt attccgtaat tccggttccc
      661 ggttgaattc tttgaagttt gattaatagg tccgagctta taattaatgc ttttcttccc
      721 actaatccct tatcaaataa atttgttatg cctgctgtta tgtcttttac ggggttttct
      781 ccttcttccc agttgctaag ctctaatttt gtaattcctt tagccgtaag aagtccttca
      841 tatcccaatt ctttattgcc gttaaaaatt aattcatctt ctttttttga aatttctgcg
      901 cttgcctgta atgcagaaga caaatcaata ggcattcctg ttttttcgct aagttcaagg
      961 tctcgccata aaaggctgaa atcttgattt aataaaggta tttgctcgaa ttttcttcct
     1021 tttatttgaa ctatatcgcc ttcagtttct gataattgtg tactgtcatc tatatttatg
     1081 ctctgtactc ctgcccctaa gggaccgtaa atatgcagga atcttcttcc cacaagtact
     1141 tttttagcca ttgatattac ttgttcatct atttttgacc aaagctctgc ggaaatcgga
     1201 gagccttctc ttgataaaaa atccatacta tcctcctatt cttttaataa gcttcctacg
     1261 gtactactgc tatttgtatc cgttgatggt gctgtctcca tgttaatatt aagctcttca
     1321 agcatttctc ttacttcttc ttctccttca ttaaggaatt ctgcttcttt agggtctaaa
     1381 tacttaagta gtgttaatag ttcacctaca tgagcttttt cttcatctct tatgtctcca
     1441 atgatttttt ttgcaacaat gtcatcggtg gcttgaatat gtgcatcata tacgtagatt
     1501 gcctccagct cacctgcaat gtttagtctt actgcttgaa taagttcatc cttagttaat
     1561 tttcgtggta cgttagcacc aaatgggttt ccaaaacctg ccatagtttc ctcctctaaa
     1621 ataaatgttt aacattaatt atataccaca aaaaagcatt tataaacatt aaataaattt
     1681 ttatttgttg aaatgggagt aatgaaaagt attaaaagat gagatccttc gctaacgctc
     1741 aggatgacaa ctaagctcag gatgacaatt aactcaggat gacaactaag ctcaggatga
     1801 caattaagtt cagaatggat aacccagctt tttacaaatt tttatataat gactgttata
     1861 accaagatta ttcgggtata agactattat gtagcttaag tatattttat atatgctgaa
     1921 tggagggata gattgaatgt tttaaattta gatttcagaa aacaaaagat gatgaggacg
     1981 gttatgttgt ctcttatccc gataattata ttttccgttt ataattatgg cttgagagtc
     2041 ttagccttgc ttactgtgat aacgattaca ggtacggctg tcgagtattt gtgggaaaag
     2101 cattataaca ataagccgtc tgaagccata tttgtaacct gcatattgta tactatgacg
     2161 cttccttcgt ccatacctta ttggattgct gttataggga ttatttttgg tgtaattttc
     2221 ggcaagatgt cttttggagg gtttggcaga aatgttttta atcctgcttt ggttgggcgg
     2281 gcttttattt atattaactt tccaaatccc atgacaatag tatggaatga agcggcaagc
     2341 ggttttccgg gcggtttttc tacatattta acagcaggta ttgatgctgt atcagaagca
     2401 actccaatga ttacatataa ttataccgga gaaattatgg atttaaattc tttgatatta
     2461 ggaaacattc cgggtgccat tggagaaagc tataaaatat tgattatatt ggctgcaatc
     2521 tacttaatat atacaaagac tgcatccctt gagattatgc tgggctgctt aatcgggttt
     2581 tttacagtcg gaacttttat gtattatata tatgatggta taaatcccat atatggaatg
     2641 atgatgggtg gatttttgtt tggtacggtt tttatggcaa ccgaccccat cagtgctgcc
     2701 aaaacaaaaa agggcaagtg gatttttgga ataataatag gtgttgtaac tgttcttatt
     2761 cgggctctgt cgctttttaa cggcggtatg atgtttgcaa tattgatggg taatacattt
     2821 gcacccataa ttgatcattt tattaaggaa gccgaaaata gaaagaaaag taaggcggtg
     2881 gcagcatgaa aaattctttt atttatccaa taatatttat gatggcggtt accgcagtat
     2941 ttatttcagt cttggcaggt ttgaatttcg ttaccgcaga tacaatttcc tttaatcaag
     3001 aaagccagct gcagcaaaaa attctttata tttttgatat attgccggaa ggcgggacgg
     3061 agaaggatat agaaagagta tttaatgata gtgtaattga aaaagagtgg ggagagctaa
     3121 gaggttatgc attgattcaa ggcggtaaag aaacagcata tgcaattcct gttaacggtg
     3181 caggactttg gggctccata ataggatata ttggattaaa tgcagattac acagaaatta
     3241 taggtattga gtttgtaaca caaaatgaaa ccccgggact tggcggcaga atatcagagg
     3301 attcatacaa ggagcagttt aggggtatag atatatccgg taagtcagaa aattttatta
     3361 tttcatctcc tgagccgggc agcaatgttg atgctattgc cggtgcaacc caaacgtcca
     3421 atgccgttgt aaagctcata aatgatgata taaagatatt tttacgggag gtggaataaa
     3481 ttgaccaaga cagaagtaaa aaagatattt aaaaagggaa tatacaccga caatccgata
     3541 tttgtgcagg taataggaat ttgctctgca ttagctgtaa ctaatttaat gataaattcg
     3601 ctgattatgg gaattgctct tgctctggta actgcatttt cttcattgac ggtatctctg
     3661 cttaaagacc acactcccaa acatattcgt atgatgatac aggttttaat aatttcggca
     3721 tatgtaatta tggttgatat ttatttgaaa gcttacatgc ctgaaataag taaagcatta
     3781 ggaccttatg taggacttat aattacaaat tgtatcataa tgggaagggc tgaaggattt
     3841 gcccggtcca atccgccaat accgtcattt tttgacggtc ttgcatccgg tataggttat
     3901 acctttgttt taatgtctat ttctttcata agagaaattt taggttttgg aagtattttt
     3961 ggaatcagtc ttattcctga aacttttaca aggtggacat taatggttat gcctccttcg
     4021 gcatttttca tacttgcatt aataatgtgg gtttttaaca acaatcgtct aaaaaaacag
     4081 agagaaggag ttgagagaaa atgattcagg tccatccttt tataatattt attgcttcta
     4141 tttttacaag caacatgatt ttttcaaatt ttttggggat gtgctcattc attgccgttt
     4201 caaaggaaat accaactgcc ttgggcttgg gaattgctgt tacaatggtg ctttcggcta
     4261 caaccgtgtt aaattatctt gtatattggt acattataat gcccttagga ttggaatact
     4321 taatgtatat attgttcatt ttggttattg cagcatttgt acagcttttg gaaatgacaa
     4381 tggacagata tttaccgact ctatactatg ccttgggaat ttttctgcct cttataaccg
     4441 ttaattgtgc catacttgga gtttcattgt tcatggttac caggcagtat aattttttcc
     4501 agacttttgg ttttgcagta ggctcaggta tcggttgggc tcttgccata actgcgatgg
     4561 cgggaataag gtcaaagctt aatgaaaact ccataccgga ggggttaagg ggagttccga
     4621 ttactttaat aattaccgga attatggcct tagcattcat cggtttcagc ggtatggttc
     4681 aaatacagta ggaggcgtag gaggcattat ggatacaata attatgacaa ttgtaatatt
     4741 ctccggaatt tcaggagttc tagcagcaat actgactatt tccgacagaa ccataggcaa
     4801 ttacggagag gttactctta cgataaatga agataaaatt tataccgtta agggcggcag
     4861 cagtttgtta gatacattaa gaaatgaaca aatttttata ccttctgcat gcggtggaaa
     4921 gggaacctgc gggtattgca aggttgttgt ccttgaagga ggaggacctg ttcttgcaac
     4981 cgaaaaacct cttcttacaa aagaggaatt agaaaataat acaaggctgt cctgccaatg
     5041 caaggttaag caaaatataa aagcaaaaat tcccgaagaa ttgttcaatg ttaaagaata
     5101 tgccgttatt gtagaaaaaa tggagcaatt aacaccgacc ataaaaaaac ttcgttttaa
     5161 tttgggagag gaagaaataa gttttaaacc gggacagtat gtccagctta aagctcccgc
     5221 ctatgaagga aatgatgaag aagtgtacag ggcatattct cttgcatctt ctgtaaatga
     5281 taaacatgct attgaattat tgataggttt cacgggagga attgccacca cctatgtgca
     5341 tcagcattta aa
//
LOCUS       DAOSOW010000080         5312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_15601_length_5312_cov_3.531672, whole genome shotgun sequence.
ACCESSION   DAOSOW010000080 DAOSOW010000000
VERSION     DAOSOW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15601_length_5312_cov_3.531672"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..1045)
                     /locus_tag="PLD67_03140"
     CDS             complement(179..1045)
                     /locus_tag="PLD67_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOT21549.1"
                     /translation="MLNELNRVIDYIEEHLTDDELSLEIISEYAGVSDYHFRKIFFYL
                     SGMTLSDYIKNRKLSEASMDLLHGEKVTDVAFKYGYQSMDGFTRAFKKWCGLLPSEVI
                     KNGISKSFPKLSFIITVKGGINMEFRIENKPAFNLVGVCKRVPMQFEGINNEIVKLAE
                     SITDEQREEMHSLQNIEPYEIVNASYEADADFLKEEGYLTHLIGVLTTKNEISDLLEK
                     VPVEAHTWAVFPNEGPFPSTLQETYAKIYSEWLPSSGYEVINAPTFSFTKMDKNKENY
                     AYSEVWTPIRKK"
     gene            complement(1134..2216)
                     /gene="rsgA"
                     /locus_tag="PLD67_03145"
     CDS             complement(1134..2216)
                     /gene="rsgA"
                     /locus_tag="PLD67_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007960505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOT21550.1"
                     /translation="MSKINMENIGLSQRFKNESTLYEGLYIGRIASQYKGLYKVITED
                     GILTAEVSGKFRFDAKTTSDYPAVGDFVMIDRNDDKDGNAIIHHVLTRKSAFVRKAAG
                     TSNEEQIVASNIDTVFICMSLNNDFNIRRIERYLGIAWDSGASPVIVLTKSDLCDNIS
                     EKLAELDTVAIGADVIVTSSMSEDGYLPIKRYLGSGKTIAFIGSSGVGKSTLINKLIG
                     EDVLETNGLRNDDKGRHTTTRREMLLIPDGGVVIDTPGMREIGIESADLTKTFADIDE
                     LSAKCKFSDCTHINEPKCAVQEAISNGLLSRDRFESYLKLKKEAKYDGLNSRQIENKK
                     LNEMFKDFGGMKNVRKMLKEKKKLER"
     gene            complement(2348..2533)
                     /locus_tag="PLD67_03150"
     CDS             complement(2348..2533)
                     /locus_tag="PLD67_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOT21551.1"
                     /translation="MKKITAIIGSARKGATYQAVREFEQNLKEYEEIDFEYIFLSDYN
                     LEFCRGCKICFDKGEAY"
     gene            complement(2627..3136)
                     /locus_tag="PLD67_03155"
     CDS             complement(2627..3136)
                     /locus_tag="PLD67_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21552.1"
                     /translation="MKSINKMRNYYLVFFVIAALLTLWFALKFIYEAAFIFGNISIVL
                     LLYVIKQNRLLYDTRLIYDNCIFKIPSATVSTSNSEIKLAVEEIVVSAFGLIIGNKIY
                     KWGYHGAYGVKLKAIEINPERVYLTFGDASKTARVELLHGITNKQEVLDIKQKFWRET
                     GIDTVVNGW"
     gene            complement(3181..5163)
                     /locus_tag="PLD67_03160"
     CDS             complement(3181..5163)
                     /locus_tag="PLD67_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21553.1"
                     /translation="MKNKIISLLLISSIIFNMVGFSGCSKAEEKLVDTSVQLPEHITG
                     TVSDENLTVSQDGVSFTIEEGSVLMDTDVEITLKKDLPPIIVGDEEIMLTAYDFSVAG
                     EEPLGVMTLEIPFTASDDEVFAAYYNLEKGEWEPVEFIYDAERKCAVIYTTHLSSYGL
                     FTVENENTRNALIAYTYNLELAEVNINEAIRMLENAMEDPDTFYEDCFDLISNSVSIH
                     SSITTNLVDVVSGLSSYVPTFSEGLKDNVTKFGYLLTGIEVVNAFMNDDKPAAVWKMF
                     DTAVGFNVDKIASLSGTASMAAGLFSVGLVSYALNKFISTALQGRTDMYERAYNLYYK
                     ENGRKTAEWVYLIRAMVENGKDPEKTGEAISDEIKRYVNEFWEDDLTIAEYGKDANPN
                     AWSSGSLGGLNDGIKKEISNYYQMTIERVVGNILNRIQIENKINMQSEMRKELFNFQK
                     KMNSIVTISVKDSKAENEPSHFAGAVIRFSELPSSIEDPEKWESKISEKGAARIQFRV
                     LAHLLAGSPRKMEIVKKSGDKEDIMVRFNAKFSLPESKLDLADLYPRSDYPKNIGGSY
                     TVSMYNTTTGKTIDDLMLKVTVTQEVSDGCYADFYLENSGSVYLNGNYFYRYATGKVN
                     YALGDMSFSGDDNNVSFKFDAYDNQKKVWGAFTGHK"
BASE COUNT         1574 a         1129 c          765 g         1844 t
ORIGIN      
        1 tgggagttac ggaaatgact aaaagcttaa attcgtacaa agatgcagac cctgatattc
       61 aggcgctggc agacaggttt ataaaattgg aacaagataa tattgacagg ttaatgaaat
      121 tcttataata aacccagaca aaaaaccccc acttttaaat agtgggggtt ttaaatgctt
      181 atttctttct tatgggagtc caaacttcgc tgtaggcgta attttcttta tttttatcca
      241 ttttggtaaa agaaaaagta ggagcgttta ttacttcata cccggaagat ggaagccatt
      301 ccgaatatat ttttgcatat gtttcttgta acgtagacgg aaatggtcct tcattcggaa
      361 acaccgccca tgtgtgagct tcaacaggaa ccttttccaa taaatcacta atttcatttt
      421 ttgttgttaa cacacctatc aagtgggtta aatatccttc ttcttttaag aaatcagcat
      481 ctgcttcata agaagcattg actatttcat aaggttcaat attctgaaga gaatgcattt
      541 cttctctttg ctcgtcagtt atactttccg caagctttac aatctcgtta tttattcctt
      601 caaattgcat ggggacacgc ttgcaaactc cgactaaatt aaacgccggt ttattttcaa
      661 ttctaaattc catattaatt cctcctttga ctgttattat aaatgaaagt ttgggaaagg
      721 acttgcttat tccatttttt attacttctg aaggcaataa tccgcaccac tttttgaatg
      781 ctcgagtaaa accgtccatt gactgataac catacttaaa agcaacatcc gttacttttt
      841 ctccatgcag taaatccatg ctcgcttccg acaattttct gtttttgata tagtcactaa
      901 gggtcattcc ggaaagataa aagaatatct ttctaaaatg gtagtcggaa accccggcat
      961 attcagaaat tatttcaaga gatagttcat catcggttaa atgttcttca atataatcaa
     1021 ttacacggtt taattcgttt aacattcatt ccccatcctt cgtcttcata ataccaaaga
     1081 tatttttata atgctcgatt tttttattat aaaaaatatc gaatcaaagg atatcaacgt
     1141 tctaattttt tcttctcttt caacatcttt cggacattct tcatgccgcc aaaatccttg
     1201 aacatttcat taagcttttt attctcgatt tgcctggaat ttaatccatc gtatttagct
     1261 tcttttttta gttttaaata actttcaaat ctatctcttg acagtaagcc gttacttatg
     1321 gcttcttgca cagcacattt tggctcatta atatgcgtac aatcgctaaa cttgcactta
     1381 gcggacaact catcaatatc ggcaaaagtc tttgttaaat cagcactttc aatacctatt
     1441 tccctcattc ccggagtatc aataacaaca ccgccgtcag gtataagaag catctctctt
     1501 ctggtcgttg tatgtctgcc tttatcatca tttcttagcc cgttagtttc aaggacatct
     1561 tcaccgataa gtttgttaat caaggttgac ttaccgacac ctgaagaacc tataaaggca
     1621 atggtttttc cgctgccaag atatctttta atcggtaaat aaccatcctc gctcatgctt
     1681 gaagtaacta ttacatctgc tccgatagca acagtatcaa gctctgccaa tttttcagag
     1741 atattatcgc ataaatcgga tttggtaagc actataaccg gacttgctcc gctgtcccat
     1801 gcaatcccta agtagcgctc aattctacga atattgaaat cattattaag cgacatacaa
     1861 ataaaaacag tatcaatatt ggaagcaact atttgctctt catttgatgt tcctgcagct
     1921 tttctgacaa aggcactttt tcttgtaagg acatgatgaa ttattgcatt tccatcttta
     1981 tcgtcatttc tgtcaatcat aacgaaatcc cctacagccg gataatcaga tgttgttttt
     2041 gcatcaaaac gaaatttccc tgaaacctct gctgttaata tgccatcttc tgttataacc
     2101 ttgtataaac ctttgtactg agaagctatt ctcccaatat aaagtccttc atataaagta
     2161 gactcatttt tgaacctttg gctgagtcct atattctcca tatttatttt actcaatttt
     2221 attcctccta atattcatat tatgttatga ctttttggag gtttatactt tattattttg
     2281 tatgcacctc cccatttact gcaatatccg ttactatctt attaaacatg aataccaact
     2341 cctttcatca atacgcttct cccttgtcaa aacaaatctt acatcctcgg caaaattcaa
     2401 gattgtagtc acttaagaaa atgtattcaa aatcaatttc ttcatactct tttaaattct
     2461 gttcaaattc cctcactgcc tgataagttg ctccttttct tgcgctgcct ataattgctg
     2521 ttattttttt cacaatattc cccctgacaa caaatacttt agattaaaac actttcttat
     2581 acctatatgc attataacat attcttccat aatcaagaag ttttttttac caaccgttaa
     2641 caactgtatc tatacctgtt tctctccaaa acttctgctt tatatccagc acttcctgct
     2701 tatttgtgat gccgtgcaat aattcgaccc tcgcagtttt tgatgcatca ccaaaggtta
     2761 aataaactcg ctccggatta atctcaatgg ctttcaattt tacgccatat gctccgtgat
     2821 aaccccattt gtaaattttg ttgcctataa taagtccaaa agcagacacc acaatttctt
     2881 cgaccgctaa ttttatttca ctgtttgatg tggagacggt tgctgatggt attttaaaaa
     2941 tgcagttatc ataaatgagc ctggtatcgt acagcagtcg gttttgttta attacataaa
     3001 gaagaagcac aatacttata ttcccaaata taaacgctgc ctcataaata aacttcaaag
     3061 caaaccatag agtcaacaaa gcagcaatta caaaaaatac aagataataa ttacgcattt
     3121 tattaataga cttcacttgt atcacacccc ttttatgtct attctgccct gtaaagagtg
     3181 ttatttatgt ccggtaaagg ccccccatac ctttttttga ttgtcatatg catcaaattt
     3241 aaagcttaca ttgttatcgt cacctgagaa actcatatct ccaagggcat aattgacctt
     3301 acctgttgca tagcgataaa agtagtttcc attcaaatat accgagcctg agttttcaag
     3361 atagaaatca gcataacaac cgtcggaaac ctcttgtgta acggtaacct tcagcatcaa
     3421 atcatcaatt gtttttcctg tagttgtatt gtacattgaa acagtatagc tcccccctat
     3481 atttttagga taatctgatc tcggataaag gtcagctaaa tcaagtttgg attcaggcaa
     3541 actgaacttt gcattgaatc tcaccataat atcttctttg tcacccgact tcttcactat
     3601 ttccatcttt ctcggactgc cggctaaaag atgagccaat actcgaaatt ggattcttgc
     3661 tgcacccttt tctgaaattt tactttccca tttctccggg tcttcaatac ttgaaggaag
     3721 ctcagaaaat cttatgacag cccctgcaaa gtgcgacggc tcattttctg ccttgctgtc
     3781 ttttactgat atagtaacta tggaattcat ttttttctga aaattaaata attctttacg
     3841 catttctgat tgcatgttga ttttgttttc tatttgaata cgatttagta tattccctac
     3901 aactctttca attgtcattt gatagtagtt acttatttct ttcttaatac catcgttaag
     3961 tcctcctaat gagcctgatg accatgcatt agggttggca tctttgccat attcggcaat
     4021 agtaagatca tcctcccaaa attcattaac atatcgctta atctcatcag aaattgcctc
     4081 gccggttttc tcggggtcct tgccgttctc taccattgct ctaattaaat aaacccattc
     4141 tgcagttttt ctgccatttt ccttataata aagattatac gccctttcat acatatcggt
     4201 tcttccttgt aatgcggtag aaataaattt atttaatgca tagcttacaa gtccgaccga
     4261 gaataaacct gccgccatag aggcagtacc gctcaaggat gcgattttat ccacgttgaa
     4321 tcctactgct gtatcaaaca tcttccatac tgcggccggt ttatcatcat tcataaatgc
     4381 attcacaacc tcaataccgg tcaataaata tccaaatttt gtaacattat ccttcaatcc
     4441 ttcactaaaa gtgggaacat atgaagacaa tccggacaca acatccacca gattagttgt
     4501 aatactgcta tgaatagaaa cactgtttga aattaaatca aaacaatctt catagaaggt
     4561 atcagggtct tccatagcat tttcaagcat tcttattgcc tcgtttatat tgacttccgc
     4621 caattccaaa ttgtaggtgt aagcaatcag cgcatttcgt gtattctcgt tttcaacagt
     4681 aaatagacca taggaagata aatgagtggt atatataaca gcgcactttc tttccgcatc
     4741 atatataaac tctaccggct cccattctcc cttttccaaa ttgtaatagg cggcaaaaac
     4801 ctcatcatct gaagcagtaa aaggaatttc caacgtcatg acacctaagg gttcctcacc
     4861 ggcaacgctg aaatcataag cagtgagcat aatttcctcg tctccgacta taatcggagg
     4921 caaatccttt tttaaagtta tttctacatc agtatccatt agcactgatc cttcctctat
     4981 ggtaaatgaa actccatcct gagaaacagt caagttttca tctgatacgg tacctgttat
     5041 atgttcaggc agctgaacag atgtatcaac aagcttttct tcggctttac tgcaaccgga
     5101 aaagccgacc atattaaata ttattgaact aattaatagt agagatatta ttttattttt
     5161 cattttgtcc tcctttttgt tatccttggc atatagtttt tcctccttta taatgcataa
     5221 gatttttgaa tatgagagtt ttgatatatc ctcttatatt ggatatttta agagagaaac
     5281 cttaataaat catcgttcga aaggatgaat tt
//
LOCUS       DAOSOW010000081         5275 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_15744_length_5275_cov_2.751724, whole genome shotgun sequence.
ACCESSION   DAOSOW010000081 DAOSOW010000000
VERSION     DAOSOW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15744_length_5275_cov_2.751724"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>961)
                     /gene="rseP"
                     /locus_tag="PLD67_03165"
     CDS             complement(<1..>961)
                     /gene="rseP"
                     /locus_tag="PLD67_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOT21554.1"
                     /translation="VFSAIVLVHEYGHYKAAKSVGIKIYEFAIGMGPTVFKKEKKGIV
                     YSIRAVPFGGFVQMEGEEEDSRTPTSFSTKTIAQRAKVIAAGPVMNFVLALVLFLIIA
                     FSYGVYGNHIDYIDENSNEYKAGLRTGDKIISVNGERTYIWEDIFYELVSPEESSYTL
                     EYERNGQIDKVNIDKNYRKIIGVTSTIVNGKTTTTVTPSDMTSPAYKAGIMDGDIVIK
                     INDIPVNTWDELTTQVENSNKTVKVTVDRKGEIIDYNIDLVEQISVRFNTQLEKSFMT
                     AISSSMYKTVYYIELMFKTIRLLVTGKLGSDALGGPVMVISMVG"
     assembly_gap    962..971
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1006..1773)
                     /locus_tag="PLD67_03170"
     CDS             complement(1006..1773)
                     /locus_tag="PLD67_03170"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOT21555.1"
                     /translation="MGRRVLTGFAGGAFIALITYLGGSLYDFVFLGITCIAIYELSKV
                     FFNDGFDYGAVMNYMFAIALFIEKITENRHFFDFFLFLYISIIFLIYIFNKKVTVKKV
                     SEIIFMGTYVVFFMYHMMLMNNSKFVWLVYIIAFGTDTFAYFTGKFFGKNKLYPEVSP
                     NKTVEGAIGGIIGSTILSLIYFNYLSINKYFYIIIFSVSASVFSMAGDLTASKIKREY
                     KIKDFGNLLPGHGGIMDRFDSVLFVAPTVYYFILHFI"
     gene            complement(1784..2506)
                     /locus_tag="PLD67_03175"
     CDS             complement(1784..2506)
                     /locus_tag="PLD67_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOT21556.1"
                     /translation="MNIEEKALSGKIPEHIAVILDGNRRWAKQKNLPVQAGHREGALR
                     VTDLVRNCGDLGVKYLSVYAFSTENWSREKKEIDYLMNLLVEFVFKELENLHKNDVKI
                     TMMGNMEDLPSKTRKEVERAIELTKNNKTLHLNIALSYGSRNEIVNAVKNIIKDYENN
                     KIDIEEINEESFKKYLFTNDMPDPDLLIRTSGELRLSNFLLYQLAYTEFYFTEVLWPD
                     FNKNELIKAIENYQQRKRRYGN"
     gene            complement(2559..2741)
                     /locus_tag="PLD67_03180"
     CDS             complement(2559..2741)
                     /locus_tag="PLD67_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21557.1"
                     /translation="MDSLIGYPLKEALQKLESLNVIINIVKVKGSNRRFNNLEKPYVI
                     KENYSDDTVTLYVSYF"
     gene            complement(2743..3264)
                     /gene="frr"
                     /locus_tag="PLD67_03185"
     CDS             complement(2743..3264)
                     /gene="frr"
                     /locus_tag="PLD67_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOT21558.1"
                     /translation="MKKSINFLKEELATIRAGKANPKLIDKIQVSYYGTMTPLNQIAN
                     ISVPEPRMLLVQPWDANSLKDIERALLTSDLGINPTNDGKVIRLIIPVLTEERRKELL
                     KLVKKEIENSKVAVRNIRRDTNEEIKRMEKQSEISKDDQKRAEDEMQKLTDKYINLID
                     EIYKDKEKEVLEV"
     gene            3582..4592
                     /locus_tag="PLD67_03190"
     CDS             3582..4592
                     /locus_tag="PLD67_03190"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT21559.1"
                     /translation="MERIKAAVIGAGIFGDTHCRVYSESPIVDLAWVCDQNQERAKQA
                     AKKYNCNFTTDLQEITNDPSISIVSVATPDFAHRDISLKVIEAGKNLIVEKPLATNVE
                     DAQAITDAVKRKGVKFMTDFQNRWNAPFIQAKQNLESGKYGEPVSAYIRLANSIMITK
                     WLSWSAKSGPQWFLGPHIVDLVCWLYNQKPIKVFATGKRKVLKSIGYDTYDALQAQII
                     FKDSFATIDTSWIVPKNWPSLDFRMDILTTNGKMELEPTFNGISMCADEGYQIPFIGG
                     RQDGFDRMFGFFKEPILHFIDHVVKDIPCLVGVEDGLINTKIVVAIEKSIESGKIIEL
                     NL"
     gene            4744..>5275
                     /locus_tag="PLD67_03195"
     CDS             4744..>5275
                     /locus_tag="PLD67_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT21560.1"
                     /translation="MKKLTLPLVILLILVCVSAVVAQEKVRLTVEMSVYVEAPHKKAF
                     DLLKEAYELQNPNVEIVYYGPAYDEYWDKLAVEIVSGTEADIVQLQDAAARYATYASL
                     REGETGAFINLDQYIKGTELEDKLIGQKELVFNGHYIGIANYATGMRGVYYRKSLFEE
                     AGIDADTIITNEDFLEV"
BASE COUNT         1650 a          923 c          848 g         1844 t
ORIGIN      
        1 tccgaccata gatattacca ttacaggtcc gcctaaggca tcactgccca atttgcccgt
       61 tacaagcagt cttatggttt taaacataag ttcaatataa tatacagtct tatacattga
      121 agaagatatg gccgtcataa atgatttttc aagttgagta ttaaatctta cagatatttg
      181 ctccaccaaa tctatattgt agtctataat ttctcctttt ctgtctacgg taacctttac
      241 tgttttattt gaattttcaa cctgggttgt cagctcatcc caagtgttta caggtatgtc
      301 attaattttt atgacaatat ctccgtccat aattcctgcc ttgtatgcag gagaagtcat
      361 atctgaagga gttacggttg tagtggtttt tccgtttacg atagttgagg taactcctat
      421 tattttccga tagtttttgt cgatattaac tttatcgatt tgaccatttc tttcatattc
      481 caatgtataa gaactttcct cgggagatac taattcataa aatatatctt cccatatata
      541 ggttctttca ccgttaacgg aaataatttt atctccggtc cttaagcctg ctttgtattc
      601 attggagttt tcatctatgt aatctatatg attaccataa actccatatg aaaaagctat
      661 tattaaaaac aatacaagag ccaacacaaa gttcatcacg ggacctgctg caattacttt
      721 ggctctttga gctatggttt ttgtactaaa gcttgtaggt gttcttgaat cttcttcttc
      781 accttccatt tgaacaaacc cgccgaacgg aacagctcta attgaatata ctattccttt
      841 tttttctttc ttaaatacgg ttggacccat tcctatggca aattcatata tctttatacc
      901 aacgcttttt gcagctttat aatgaccata ttcatggaca agaacaatag cagaaaaaac
      961 annnnnnnnn nacgctatta atgttaacaa tagaataatc tccgtttata taaaatgcag
     1021 aataaaatag tacactgtag gtgcaacaaa taaaacactg tcaaatctgt ccattatgcc
     1081 tccgtgaccg ggcagaaggt tcccaaaatc ctttattttg tattctcttt taattttaga
     1141 tgctgtcaaa tcgccggcca ttgaaaatac agatgcactt acactaaata ttataatgta
     1201 aaaatattta tttatgctta aataattaaa atatatcaat gataaaatcg tgcttccgat
     1261 aataccgccg attgcaccct cgacagtttt attcgggctt acctcaggat ataatttatt
     1321 tttgccgaaa aatttaccgg taaaatatgc aaatgtgtca gtgccgaaag ctataatata
     1381 taccaaccat acaaattttg aattattcat aagcatcata tgatacatga agaataccac
     1441 atatgtaccc atgaaaatta tttctgaaac tttttttaca gttacttttt tattgaatat
     1501 atatattaaa aatattatag atatgtataa aaacaagaaa aaatcaaaaa aatgtctgtt
     1561 ttcagtaatt ttctcaataa ataaagcaat tgcaaacatg taattcataa cggctccata
     1621 atcaaagccg tcattaaaaa aaacttttga taattcataa atagctatgc atgttatgcc
     1681 caaaaaaaca aagtcatata aggagccgcc taaatatgtt ataagtgcta taaatgctcc
     1741 gcctgcaaaa ccggttaaca ctcttctacc catagtacac cgcctaattt ccgtatcttc
     1801 tttttctttg ctgatagttt tcgattgctt taattaattc atttttatta aaatcaggcc
     1861 ataaaacttc cgtgaaataa aattccgtat atgcaagctg atacaagaga aagttgctta
     1921 accttaattc tccgctggtc ctgattaata aatcagggtc tggcatatca ttagtaaata
     1981 agtatttttt aaagctttcc tcattaattt cttctatgtc aattttattg ttttcgtaat
     2041 ctttaattat attttttact gcattcacta tctcatttct tgagccgtag ctcaatgcaa
     2101 tattcagatg aagtgttttg ttgtttttag taagctcaat tgcgcgctct acctcttttc
     2161 tcgtcttgct tggcaaatcc tccatattcc ccatcattgt tatttttaca tcgtttttat
     2221 gaaggttttc aagctcctta aatacgaatt caaccaagag attcattaaa taatctattt
     2281 cttttttttc tctgctccaa ttttcagttg aaaaagcata aacagataga tattttacac
     2341 caaggtcacc gcaatttctt actaaatcag taactcttaa agctccttct ctatgtcctg
     2401 cctgtacggg caggtttttt tgttttgccc atcttctgtt tccatctaat ataactgcaa
     2461 tatgttcagg tattttccct gacaacgctt tttcttcaat attcataata aacctcacaa
     2521 ttccaaaagt acggggtaca tagaggtgtc cccgtacctt aaaagtaaga tacgtacaat
     2581 gttacggtat catctgaata gttttcttta attacatatg gtttttctaa gttattgaat
     2641 cttctattac tgccttttac tttcactata ttaataataa catttaaact ttctaatttt
     2701 tgaagtgctt cttttaatgg ataaccgatt aaactatcca acttatacct ctaatacttc
     2761 tttttcttta tctttatata tttcgtctat aaggtttatg tatttgtcag taagcttctg
     2821 catttcatct tctgcccttt tttggtcatc tttggatatt tcgctttgtt tttccatcct
     2881 tttaatttct tcgttagtat ctcttctgat atttcgtact gcaacttttg aattctcaat
     2941 ttctttttta accagtttta aaagttcttt tcttctttct tccgtaagca ccggaattat
     3001 cagccttata acctttccgt catttgtcgg gttaattccc aagtcggaag ttaacaaagc
     3061 tctttcaata tcttttaatg aatttgcatc ccaaggctga acaagaagca ttctcggttc
     3121 cggtacggaa atattagcta tttgatttaa tggtgtcatt gtaccgtaat aacttacttg
     3181 aattttatca attaatttcg gattagcttt gcctgctctt atggttgcaa gctcttcctt
     3241 aagaaaattt attgattttt tcattataat tcccaatgaa aaatttcaat ttaaaaataa
     3301 gcgatgaaaa tcctttggtt cggatcattt ttttgaattt aaaaaatacg gtattaagta
     3361 ataaaatgga ggtaaaaaca aattcatgaa gaaaatatta tgttgttcct taccgttaat
     3421 attatcgcaa aataaaatag tttatgttag actaattaac aatagaaaga taaaaatgga
     3481 tattggagtg ctgataaata tgatcatata gggtaaattc ttatacctga ttgacatggc
     3541 tgcaattatt tttaacgtta aagaaaaagg aggaagtctt tatggaaaga attaaggcag
     3601 cggttatagg agctggtata tttggagata ctcattgtcg ggtatattca gaaagcccca
     3661 tcgtagattt agcttgggtt tgcgatcaaa accaggaacg agcaaaacaa gcagcaaaaa
     3721 aatataattg caattttact actgatcttc aagaaataac caatgatcct tcaattagta
     3781 ttgttagcgt agcaactcct gattttgccc atcgagatat tagcttaaaa gttatcgaag
     3841 ccgggaaaaa ccttattgtt gaaaaaccac tggcaaccaa tgttgaggat gctcaggcca
     3901 tcaccgatgc agtaaaacga aaaggcgtca aattcatgac cgattttcaa aaccgctgga
     3961 atgccccttt tatacaagcg aaacaaaacc ttgaatcagg aaaatatggt gaaccagtga
     4021 gtgcctacat tcgcttggct aacagtataa tgattaccaa atggttatct tggagtgcaa
     4081 aatccggacc tcagtggttt ttgggacctc acatagttga cctggtttgt tggttgtata
     4141 accagaaacc aattaaagtt ttcgctaccg ggaaacgcaa ggttttaaaa tcaattggct
     4201 atgatactta tgatgccctt caagctcaaa ttatttttaa ggattctttt gctactatcg
     4261 atacttcatg gattgtccca aaaaactggc cttcacttga ctttcgaatg gatatactta
     4321 caaccaatgg aaaaatggaa ttagaaccta cctttaatgg gatttcgatg tgtgctgatg
     4381 aaggatacca aatacctttt attggaggtc ggcaagacgg tttcgatcgc atgttcggtt
     4441 tctttaaaga acctattttg cattttattg atcatgttgt taaagatata ccctgtttag
     4501 tgggagttga agatggactc attaatacaa aaattgtggt agccattgaa aaatctattg
     4561 agtcaggtaa gattatcgag cttaatctat agcttttttt agttattttg taagaaataa
     4621 atctgcgaat atggaggtgg taatgtaata taaaaaaata aagtgataga tgagggtttg
     4681 gtattttttc aaaaagtgtt tttaatcatg gagttagaat ttttgcaata taaggaggca
     4741 gaaatgaaga agttaacctt accattagta atattgttga ttctggtttg tgtttctgca
     4801 gttgtagccc aagaaaaagt ccgcctgacg gttgagatgt cggtgtatgt tgaagccccc
     4861 cacaaaaaag cttttgattt attgaaagaa gcgtatgaat tacaaaatcc aaatgtagaa
     4921 attgtgtact atggcccagc ctatgatgaa tattgggata agcttgctgt cgaaatcgtt
     4981 tccgggactg aagcagatat tgtccaatta caggacgctg cggcacgata tgccacctat
     5041 gcatcattac gcgaggggga aactggagct tttattaact tagatcaata catcaaggga
     5101 acggaattag aagataagct gattggtcaa aaagaattgg tttttaatgg tcattatatt
     5161 ggtatcgcta actatgcaac gggaatgcgc ggagtatatt accgcaagtc attattcgaa
     5221 gaagctggaa tcgatgcgga taccatcatt accaatgaag attttctcga ggtcg
//
LOCUS       DAOSOW010000082         5272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_15760_length_5272_cov_3.596320, whole genome shotgun sequence.
ACCESSION   DAOSOW010000082 DAOSOW010000000
VERSION     DAOSOW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5272
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15760_length_5272_cov_3.596320"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="PLD67_03200"
     CDS             complement(<1..450)
                     /locus_tag="PLD67_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006532610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOT21561.1"
                     /translation="MAGLVPYNRRNRGLVDRGFSDFRDFYNMLDDFFYDAWPSKKYFA
                     RDTFKIDVQENDNEYLVEAELPGVNRDEVNIEMNEGRLNISIQREESINEENKNYVHK
                     ERRFSSMSRSLYLDDAKSTGIKAKLDNGVLSIIVPKENKPNKSVKIDI"
     gene            complement(656..1147)
                     /locus_tag="PLD67_03205"
     CDS             complement(656..1147)
                     /locus_tag="PLD67_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOT21562.1"
                     /translation="MFIKKNQFYIIIAVIVFLMAVYMIYSIRKDNRLNNNEENVIKLV
                     EDFGSTLKNVPLLAPRDEVIQAIKDNYSPYVTDELMQNWIAHPRRAPGRTTSSPWPDR
                     IEINEIERLSEDEYSIKGRIVEVTSTEAEGGGAAAFRPVELILKRAGNKWLIDKIIIK
                     NYE"
     gene            complement(1203..2102)
                     /locus_tag="PLD67_03210"
     CDS             complement(1203..2102)
                     /locus_tag="PLD67_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT21563.1"
                     /translation="MESVNKYKYMGFILLQSVIYGFGNPLTKIAFKSITPFWSLSVRF
                     TIAFIVFMLFFGRRIIGSLKSVEPKVYLPAGICMATAYITCNLALSWTSATNVGFLMS
                     LPVIFAPILALLIYKVKYRLIHIPVQIVVVIGLYLLCFDGEKLVFGKGEITALITAIS
                     IAGALVFGEESLKQLDAVAVSAVQAGITALISLPCALIIENTEILVHVTIPAWLVVLY
                     LAIFCTNIAYMLQNSALKYISASTVSMLQCTQPILTAIISYLLLREKLSTLGIIGAAI
                     IILCIIAENIITTKEQSASKNLV"
     gene            2505..3380
                     /locus_tag="PLD67_03215"
     CDS             2505..3380
                     /locus_tag="PLD67_03215"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOT21564.1"
                     /translation="MTLQQLHYFRVLAKVENYTKAAGILHIAQPSLSYSISQLESELN
                     IKLFERHGKKIKLSNPGELFLKYVEKSLDILDEGTIMLKTVADSSMGEVSLGYIYSIS
                     SKFIPEMIKKFYENNSNRLIKFSLVQNLGEQLIRDLKSNKIQLAFCPTPDKSLKCVKI
                     FSQQLHLIVPKKHPFSQRKCIEISDIIDEPFVALNKSSSLRSTVDEIFARYNFSPKIV
                     FEAEECNAVISFVSLNFGISIVPEIPSMNKHVNTVKICNMPLKREIYLCWIDDKYLTP
                     AAIKTRDFIIENFTV"
     gene            complement(3525..4604)
                     /locus_tag="PLD67_03220"
     CDS             complement(3525..4604)
                     /locus_tag="PLD67_03220"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOT21565.1"
                     /translation="MKKLLILLLIATMALLPVACTQPSTPTPPEQPEQPEQPEQPEQP
                     GGFDALEPVVLIGADNAGVGAAAQLYGELITEKVKEITGGKLTIDYFPNSQLGNDQEL
                     QGQMLAGDIDFVICQTAQTVSFVPEVAIFDLPMVFAKYDAETIDYALNKSEFTDKINQ
                     AYKAKNMYCLHFLQGGTFRETTSNKKIETIDDFNGLKIRTMENKNHMAFWQAMGAAPT
                     PLPWPEVYISLQQGLVDAQENATDTCVGANLQEVQDYLILTHHILYCNQFLINASKFD
                     SLDPLYQEALQQAIDEAAVEIEAQLTEIDVTNRQTLVDGGMELVEFDTSFIDQVLEKA
                     KGVYESIGNDIGQEYVDSLLNALEK"
     gene            complement(4644..>5272)
                     /locus_tag="PLD67_03225"
     CDS             complement(4644..>5272)
                     /locus_tag="PLD67_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014127999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOT21566.1"
                     /translation="KESFWALLSPIIVLGGIYSGYVTPTEAACISVFYSLFVSLIVYK
                     SFTVKEIIPFLAKAVKTYAPLCFVLAFAIAFGRVLALAKAPAIIEGFILDNFTTSITV
                     LTGIVIVFLILGMVMDTGPAIVILSPILLPAVQALGVHPVHFGVIMVCCLSIGLATPP
                     FGLDLFVAGTLSDTPPMTVAKKAIPFIIAFGIVLFVITYVPWLSLALL"
BASE COUNT         1621 a          997 c          866 g         1788 t
ORIGIN      
        1 aatatcaatt tttactgatt tgtttggttt gttttcctta ggaacaataa tgcttaatac
       61 tccgttgtca agttttgctt taatgccggt agactttgca tcatctaaat atagacttct
      121 actcatagag ctgaaacgtc tttccttatg aacgtaattt ttgttttctt cattgatgct
      181 ttcttctctt tgtattgaaa tattcagtct tccttcgttc atttcaatat tgacttcatc
      241 cctgttaaca ccgggaagtt ctgcttctac taagtattca ttgtcatttt cctgtacgtc
      301 gattttaaat gtatctcttg caaagtattt tttagatggc caggcatcgt agaaaaagtc
      361 atcaagcatg ttgtaaaagt ccctaaaatc gctgaaacct ctgtcaacca aacctctgtt
      421 ccttctgtta taaggtacta atccagccat gttcaacact cctttatctc tattgttgtt
      481 agcacttaaa ctcgttgagt gctaatcctt aaatgtaata taacattgtt tctaacattt
      541 gtcaactacc tttagaaaaa atattttatt attttttacc tttttcttac gcttgtctaa
      601 ttttgtttac tattagtcgg acaaacataa tgatttttcc aagattttct gtttgtcatt
      661 cataattttt tattattatt ttatctatta gccacttgtt tcctgccctt tttaaaatca
      721 attcaacagg tctgaaagca gcagccccac cgccttcagc ctcagtgctt gtaacttcaa
      781 caattcttcc ttttattgaa tattcatcct cagacagcct ttcaatttca ttgatttcaa
      841 tcctgtcagg ccatgggctc gatgtagttc ttcccggagc cctccttgga tgagcaatcc
      901 agttttgcat aagctcatcc gtaacatatg gactataatt atctttaatt gcttgtataa
      961 cttcatctct tggtgccaaa agaggcacat tcttcagagt gcttccaaaa tcctctacca
     1021 atttaattac attttcttcg ttattgttta aacgattgtc cttcctaatg gaataaatca
     1081 tatatacagc cattaaaaaa actataactg caatgataat ataaaactga ttttttttaa
     1141 taaacataaa tatcccccct atttatgttt atacagaaag ggattagaaa attactattt
     1201 ttttagacaa gatttttgga agcagactgt tcttttgtag taattatatt ttctgcaatt
     1261 atacataaga ttattattgc agctcctata attcctaaag tgctaagctt ttctctaaga
     1321 agcaaataag aaattattgc cgttagaatg ggctgtgtgc attgaagcat tgacacagtt
     1381 gatgctgata tgtattttag agctgaattt tgaagcatgt aggcaatatt ggtacaaaat
     1441 atcgccaaat acaaaaccac taaccaggct ggtatagtaa catggactaa aatttcagta
     1501 ttttcaatta tcaatgcaca gggcaggctt attaaagcag ttatgccggc ttggacagca
     1561 gatacggcta cggcatccag ctgcttaaga gattcttctc caaataccag tgctcctgct
     1621 atggatatgg ctgtgattaa tgcggtaatc tctccttttc caaaaactaa tttttctcca
     1681 tcaaagcaga gaagatataa tccgataacg actacaattt gtacaggtat gtgtatcagc
     1741 ctgtatttaa ccttatatat caagagtgca agtattggtg caaatattac aggcagactc
     1801 attaaaaatc cgacatttgt cgctgaggtc cagctcaatg caagattaca cgtaatgtaa
     1861 gctgttgcca tacatatgcc ggcaggtaga taaacctttg gctctacgct ttttaaagag
     1921 ccaattatac gccttccaaa aaataacatg aaaactataa aagcaattgt gaatcgaact
     1981 gaaagactcc aaaagggagt aatgctttta aaagcaattt tagtaagtgg atttccaaac
     2041 ccgtaaatta cactttgaag caatatgaaa cccatatatt tgtatttgtt cacactttcc
     2101 atttcattcc tgctttccat agaaattcta aattctcagg attttgaaat tctgccaccc
     2161 tgttattcaa taaccgtata tctatttgag acaaagacaa gatgtatcat cttaatgcaa
     2221 acgttttaaa tattatatta taaaaataaa aatcattcaa tattttgttt tacactaaga
     2281 tttacctaca gtactctcaa cttgtcactg ctcaaaccct gtcaatctgt catccttgag
     2341 gttcttaccc ttcggttcat gatgatagta tcatagcaat gacaaaattt ttttaaaatt
     2401 atagagacat tctataaata atctggtata atagctataa ttattaagcc gcacttagta
     2461 ttaacatctt accgcttatt ttgatattag gaggtttggt tgagatgaca ttacaacaat
     2521 tgcattattt tcgtgtttta gcaaaagtag agaattatac gaaagctgcc ggaatacttc
     2581 atattgccca accaagctta agttattcaa tttctcaatt agaaagcgaa ttaaatatta
     2641 agttgtttga aagacatggg aaaaagataa aactaagtaa tcccggtgag ctgtttctta
     2701 aatatgttga gaaatcattg gatatattgg atgaaggcac tataatgcta aaaaccgttg
     2761 ccgattcttc aatgggagag gtgtctcttg gatatattta tagtataagt tcaaaattta
     2821 tccctgagat gattaaaaaa ttctatgaaa acaattcaaa ccgcttaata aagttttcat
     2881 tggtccagaa tttgggtgaa cagcttataa gggacttaaa aagcaataaa atccaactgg
     2941 cattttgccc gacacctgat aaaagcctta aatgcgtgaa aatcttcagt caacagcttc
     3001 atttgattgt gccgaaaaag catccctttt ctcaaagaaa atgcattgaa atttctgata
     3061 taatcgacga accgtttgtt gctttaaata aaagcagctc tcttaggagc actgttgatg
     3121 aaatatttgc acggtataat ttttctccta aaatagtttt tgaagctgaa gaatgcaatg
     3181 ctgtcatttc atttgtttct cttaatttcg gaatttcgat tgtaccggaa ataccatcca
     3241 tgaacaaaca tgtaaacacg gtaaagattt gtaatatgcc cttaaaaaga gagatctact
     3301 tgtgctggat tgatgataag tatttaacac ctgccgcaat taaaacaaga gattttatta
     3361 ttgaaaattt cactgtctaa atgaagaaga ttcctatttt atcaaagtta tattcattca
     3421 ataaaattaa atttttgtag tgaatagagg gtactaaaac cgccggctgt tgccggcggt
     3481 cttaatctac ctgttcagtt tatttgctta gtcagataag ttttttattt ttcaagtgca
     3541 tttagcaaag aatccacata ttcctgtccg atatcattgc ctatagattc atatacaccc
     3601 tttgcctttt ctagaacttg gtctatgaaa ctcgtatcaa attctactaa ttccataccg
     3661 ccgtctacta atgtttgtct gtttgttacg tctatttcgg ttaattgagc ttcaatttct
     3721 acagcagctt catcaatagc ttgttgcagt gcttcttggt acaaggggtc aaggctgtca
     3781 aatttgctgg cattgattaa gaactgatta caatacagta tatggtgggt cagaatcaga
     3841 taatcctgta cttcctggag gtttgctccc acacaagtgt ctgtggcatt ttcctgtgca
     3901 tcaaccaatc cctgctgcaa tgaaatatat acctccggcc atggaagcgg tgtaggtgca
     3961 gcacccattg cctgccaaaa tgccatgtgg ttcttgtttt ccattgttct tatctttaag
     4021 ccgttgaaat catcaattgt ctcaattttc ttgttagatg ttgtttctct gaatgttcct
     4081 ccctggagga agtgcagaca gtacatattt tttgccttgt aagcttggtt tattttatct
     4141 gtaaactcgc ttttgtttaa tgcataatca atggtttcag cgtcgtattt tgcaaatacc
     4201 atcggaaggt caaaaatcgc tacttcagga acgaaagata cggtttgcgc tgtttgacaa
     4261 attacgaagt caatatctcc ggcaagcatc tgaccttgga gttcctggtc atttccaagc
     4321 tggctgtttg ggaaataatc aattgtaagc ttacctccgg ttatttcttt aaccttctcg
     4381 gtaataagtt ctccatagag ctgagctgca gcaccgactc ctgcattatc agcacctatc
     4441 aagactacag gttcaagtgc atcaaagcct ccaggttgtt caggttgttc aggttgttca
     4501 ggctgttcag gttgttcggg gggagtaggt gtactcggtt gagtacaagc taccggcagc
     4561 aacgccattg ttgctatcaa caataatatc aataattttt tcatttttct ccttcctttc
     4621 tttttaaata tatacttgat cctttacagg agtgcaagac tgagccatgg tacatatgtt
     4681 ataacaaaca acactatacc gaatgcaatt ataaaaggta tagctttttt tgcaacggtc
     4741 attggagggg tatctgataa agtccccgcc acgaataaat ccaatccaaa tggtggtgtt
     4801 gccaaaccta tcgataagca gcaaaccatt ataacaccaa agtgtacggg atgcactcct
     4861 aaagcttgaa ctgccggcag taatataggt gataatatta ctatggcagg tcccgtatcc
     4921 ataaccattc caagtattaa aaatactata actatacccg tcaatacggt aatacttgtt
     4981 gtaaaattgt ctaaaatgaa tccttcaata atagccggtg ccttagctaa tgccaagact
     5041 cttccaaatg caattgcaaa cgcgagtaca aagcaaagcg gtgcatatgt ttttactgct
     5101 tttgccaaaa aaggaattat ttctttaacc gtaaatgact tatatacaat caatgaaaca
     5161 aacaaagaat aaaacactga aatacaagca gcttcagtag gtgttacata acctgaataa
     5221 ataccgccta gtacaataat cggtgatagt aatgcccaga aactttcttt aa
//
LOCUS       DAOSOW010000083         5189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_16119_length_5189_cov_3.618621, whole genome shotgun sequence.
ACCESSION   DAOSOW010000083 DAOSOW010000000
VERSION     DAOSOW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16119_length_5189_cov_3.618621"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PLD67_03230"
     CDS             <1..764
                     /locus_tag="PLD67_03230"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOT21567.1"
                     /translation="AMIFNNSINNGMNQIAQPERRFGPITIGRLAPDFIALSTQGYIR
                     LSDYRGKRVILTSHPSAFGAVSTTEIISAAQDYRQLSANNIEIIGLTTDNISAILAWI
                     NDIYQNTGISIPFPIISDTDLSLSELYGMISPDRAFGSTVRSTFIIDPSGHVRAILTL
                     PISTGGSREEFYRVLQSLLIYDNYNLYTPANWKPGDPLVVPNPLSYEEMVARAENSES
                     LGYYCPFWYLCYTNINTENTNTSAPSQVDNNAAPS"
     gene            complement(914..1810)
                     /locus_tag="PLD67_03235"
     CDS             complement(914..1810)
                     /locus_tag="PLD67_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOT21568.1"
                     /translation="MKNKIKITTIFLIIIMLLTTAAGCTGSNEKNLEIRVGGKNFTEQ
                     YIMAEMFSILIEENTDLNTTLQTNLASTVLFEAIKADEVDLYLEYTGTGLINLGMEPL
                     TDPDEVYEIVKTEYDKQFNIKWMEPYGFNNTYAMMVTRETANKYGLETVSDLAEVAHE
                     MTLGGSYVFTERADGYPGLSKHYDFEFDVVIGLDPSLMYQALIEGSVDVISGFATDGR
                     IVAFDLVSLIDDKQFFPPYDATVIIRGEVLEKHPELEEILNRLANRIDDNKMAELNAA
                     VDLDKREPADVAREFLEKEGLI"
     gene            complement(1830..2462)
                     /locus_tag="PLD67_03240"
     CDS             complement(1830..2462)
                     /locus_tag="PLD67_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT21569.1"
                     /translation="MQLLDYVQNNMNNIIRLTIEHLQITGISIITAIVIGVPLGIIVT
                     RYKRLADPILASANIFQTIPSIAFFGILIPFMGIGKETAILVLFLYALLPIIKNTYTG
                     INEVSPSLIDAGRGMGMTNFQILRMVELPQSLAVIMAGVRIATVINIGSATIAAYIGA
                     GGLGELIFKGIQMYRNDMILVGAILAALLAVVADKLLGYVEHKLTPRGMK"
     gene            complement(2489..3592)
                     /locus_tag="PLD67_03245"
     CDS             complement(2489..3592)
                     /locus_tag="PLD67_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21570.1"
                     /translation="MISFKNVTKKYGDSVAVDNLTVNFKSGETVVLIGPSGCGKTTTL
                     RMINRLIEPTEGSIYINDTDISKINPVELRRNMGYVIQQIGLFPHMNIAQNVGLVPYL
                     KDWSESKRKQRVEELLDFVGMPPSKFYHRYPNELSGGQQQRIGVARALAADPEIILMD
                     EPFGALDPITRSALQDELLQMQDKLGKTIVFVTHDMDEALKLADKIVIMKDGKVLQFD
                     SPEHILKNPANEFVEDFIGKDRLLKRPEYIKVKDIMIKKPVTIRPERTLTQALEKMRL
                     HKVDSLMVVDEFGNFIGIATSNDILDNFGNGGTVIEITRTEFNHVSEDANVSQVLSIM
                     AQGQVGYVPVIDDEKHLLGLVTRSSLVNVLGRA"
     regulatory      complement(3726..3860)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            complement(3936..4932)
                     /gene="guaA"
                     /locus_tag="PLD67_03250"
                     /pseudo
     CDS             complement(3936..4932)
                     /gene="guaA"
                     /locus_tag="PLD67_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965987.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
BASE COUNT         1558 a         1097 c          812 g         1722 t
ORIGIN      
        1 atgccatgat atttaataac agtataaata acggtatgaa tcaaattgct caacctgaaa
       61 gaagatttgg gcctattact ataggcagat tagcacctga ttttatagca ctgtcaacgc
      121 agggttatat cagattgtcc gattatagag gtaaaagggt aatattaacc agtcacccgt
      181 ctgctttcgg tgcggtcagc actacagaaa taattagtgc tgcacaggac tatcggcagt
      241 tgtcagcgaa taatatagaa attatcggtt taacaacgga taatattagt gcaattttag
      301 cctggataaa tgatatatat caaaatacgg gaatatcaat tccttttcca ataatttcag
      361 atactgattt atctttatcg gaattgtatg gcatgataag tcctgacaga gcatttggat
      421 caactgtaag aagtactttt ataatagacc cgtcaggcca tgtaagagca attcttacct
      481 tgcctatatc taccggagga agcagagaag aattttatag agtacttcaa tcgttactga
      541 tatatgataa ctataacctc tatactcctg caaattggaa accgggagac ccgcttgtag
      601 tacctaatcc tctttcatat gaagaaatgg ttgcaagagc tgagaatagt gaaagtctgg
      661 gatattattg tccgttttgg tacttatgtt atacaaacat aaatactgaa aatacaaata
      721 cttccgctcc ttcgcaggtt gataataatg ctgctccttc ataaaataac aatacaatct
      781 gactttattt taaaatgcaa aaaatagatg gttagatatt aatcatacca tctattacat
      841 ttcatactgc ataaattaca tatttacaag atagttatta cattaaaatg tttttacgca
      901 tccaggtaga atctcagatt aaaccttctt tttcaagaaa ttctcgtgct acatcggcag
      961 gttctcgctt atccaaatct actgcagcat tgagctcagc cattttatta tcatcgatac
     1021 gattagctaa tcgatttagg atttcttcta actcaggatg cttttccaaa acctctcctc
     1081 taatgataac cgttgcatca taaggcggga aaaattgctt atcgtctata agagatacca
     1141 aatcgaatgc tacaatcctt ccatcagtag caaatccgct tattacatct acgcttcctt
     1201 caataagtgc ctgatacatc aatgacgggt ccagtcctat aacaacatcg aattcaaaat
     1261 cgtagtgttt gctcaaaccc ggatatccat cagccctttc cgtaaaaaca tagctgcctc
     1321 caagtgtcat ttcatgtgca acttccgcca aatcggatac agtctccaag ccgtatttat
     1381 ttgcagtttc acgagtcacc atcattgcat atgtattgtt gaatccatac ggctccatcc
     1441 atttaatatt aaattgctta tcatattccg tttttacaat ttcatatact tcatccgggt
     1501 cagtcaacgg ttccatccct aaatttatca gacctgttcc cgtatattcc aaatataaat
     1561 ctacttcatc agcttttatt gcttcaaaaa gtacggttga tgccaaattt gtctgaagag
     1621 tggtatttaa atcagtattt tcttcaataa gaatactgaa catttcagcc ataatgtatt
     1681 gttccgtaaa gttttttcca ccgactctta tttccaaatt tttttcattg ctgccggtac
     1741 aacctgctgc agttgtcaat aacataataa ttattaaaaa tattgttgtt atttttattt
     1801 tgttcttcat cgaaatacct cctttaatat tatttcattc ctcttggagt aagcttatgt
     1861 tctacatatc cgagcaactt gtctgctacg acagctaaca aggctgcaag gattgctcca
     1921 accaatatca tatcattacg atacatttga attcctttaa aaattaattc ccctaatcct
     1981 ccggctccaa tatatgctgc aatggtagcc gagccaatat ttattacggt agcaattctc
     2041 acccctgcca taattacggc taatgactgg ggcagttcaa ccatgcgcag tatttgaaaa
     2101 ttggtcatac ccattcctct gcctgcatca atcaaagatg gcgatacttc attgatgccg
     2161 gtataggtgt tttttattat aggaagcaag gcataaagga aaagcaccaa aatagcagtt
     2221 tctttgccaa tacccataaa gggtattaga atcccgaaaa atgcaatgct tggaatggtt
     2281 tgaaaaatat tagcacttgc caatatcggg tcagccagtc gtttataacg ggttactata
     2341 atacctagcg gtactccgat aacaatagct gtaattattg aaattcctgt tatttgtaaa
     2401 tgctcaatcg ttaatcgtat tatattgttc atattatttt gtacataatc caacagttgc
     2461 atatttatca ctcctttcca tcaaacagct atgctcttcc taatacatta actaagcttg
     2521 acctggtaac aagccccaac agatgctttt catcatcaat taccggcaca tatccgactt
     2581 gaccttgagc cataatggat aatacttgac tgacatttgc atcttcactt acatgattaa
     2641 attcagttct tgttatttca ataactgttc ccccattacc aaagttatca agtatatcat
     2701 ttgatgtggc tattccaata aagttgccaa attcatcaac aaccatcaaa ctgtcaactt
     2761 tatgcaggcg catcttttcc aatgcctggg taagagtacg ctccggtcgg atggttacag
     2821 gcttctttat cataatatct ttaactttaa tatattcggg acgttttaac agcctgtctt
     2881 ttccaataaa atcctccaca aattcattgg ccggattttt taaaatatgc tcagggctgt
     2941 caaactgaag aactttacca tctttcatta ttacaatttt atcggcaagc ttcaatgctt
     3001 catccatatc atgggtcaca aatacaattg tctttccgag cttatcctgc atttgcagca
     3061 gttcatcctg gagagcagac ctggtaatag ggtcaagggc tccaaaaggc tcatccatca
     3121 atataatttc agggtcggca gccaaagccc ttgctacgcc gatacgttgc tgctgccccc
     3181 cgcttagctc attaggatag cgatggtaaa atttagatgg cggcataccc acaaaatcaa
     3241 gcagctcttc aacgcgttgt ttgcgttttg attcactcca gtctttaaga tatggaacga
     3301 gccccacatt ttgagcaata ttcatatgag gaaaaagacc gatttgttgt attacatagc
     3361 ccatattcct cctaagctcc actggattaa ttttgctgat atcagtatca ttgatataaa
     3421 tacttccttc cgtaggctct attaaacgat taatcatacg caaggttgtt gttttgccac
     3481 aaccgctggg tccgattaat actacggttt ctcctgattt aaaatttacc gtcaaattgt
     3541 caacagctac agaatcacca tattttttgg ttacattttt gaaacttatc aaaataaacc
     3601 cttccttctc cattattatg ttcattgatt tgaactatct caaaatcaat ttcgaaatga
     3661 taaaataaaa ataaagccat ggagattcat agctttatcg aataatcaat aagcttttat
     3721 actatcaatc ccccttcata cgcttacgag gttagctgac ggattcggac tgaagagtag
     3781 ccctacttca taaagaagat tcaccccaaa aagttggttc ccccgctcca tccacatgtt
     3841 ggattcagcg ttgtataaaa aagaaaaatt atttaattag tttaataatt ataatctaaa
     3901 agtatattaa agtcaagaaa taataatcga ctaatttatt caaattcaat cgttgcaggc
     3961 ggcttagcag tgatatcata cacaactctg tttacattct caacctcgtt aactattctg
     4021 ctgcttactt tttccaagac ttcatagggt attcttgccc actcggaagt catgccgttt
     4081 gtagaatgaa ctgctcttag ggcaattgtg taacaatatg ttctttcatc tccggttacg
     4141 cctacgcttt tgatgtcggg aagggctgca aagtattgcc atattttatt ttgaaggcct
     4201 gcttttttta tttcatctct gaaaataaag tcagcttctc ttactatttt taatttttct
     4261 tcggttactt ctcccaatac acggattcca agacccggtc ccgggaatgg ctgtctttca
     4321 actatctctt caggcagacc caactctctt ccaactagcc gaacttcatc tttatacaat
     4381 tgtctcagag gctcaataag ttcaaagtca acatcatcag gaagccctcc cacattgtga
     4441 tggcttttga ttactgaagc cgcagatgtt ccgctttcta taacatccgg ataaatcgtg
     4501 ccctgaacaa gatagtcgat gtgtccgaat gtgttcttaa gtttctcttt ttcttcttca
     4561 aacaccctta taaactcttc gcctattatt tttctcttct tttcaggctc agttacacct
     4621 gccaattttc tgaggaatct gtcctgagca ttcacacgaa tcagattcat gtctaattta
     4681 tctttaaata ctttttccac ctgatcagct tcgtcttttc ttaaaagtcc atggtcaaca
     4741 aatacacata ttaaattttt acctattgct ttgtgtacca ttgttgcagc taccgatgaa
     4801 tcgacacctc ccgacaaggc gcaaagagct tttttgtctc caacttgttt ttttatttgt
     4861 aaaattgaat cttcaacaaa atttctgaca ttccagtctt ttctcatttg agtaccccta
     4921 atttttaaag tttattaagc tttgccatca tgcaaagtat ctcagacttt gagattcttc
     4981 attgtgttca gaatgaaaag ctatacctga catcaataag attatcaaat aaaatataag
     5041 gtgtcaataa aaaggacatg agatccttcg ctatagctca ggatgacaac ctatggttga
     5101 gtgtaacaat aactcaggat aacaatgccc ctcttgttta cataattggt aattatgtta
     5161 acctcgtata tcgaagtatg ggttttctt
//
LOCUS       DAOSOW010000084         5165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_16211_length_5165_cov_3.060665, whole genome shotgun sequence.
ACCESSION   DAOSOW010000084 DAOSOW010000000
VERSION     DAOSOW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16211_length_5165_cov_3.060665"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="PLD67_03255"
     CDS             <1..760
                     /locus_tag="PLD67_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21571.1"
                     /translation="PLKAKAQIIGLPISVSQRGYYGDLNKTIEIIKKRKGLKIILNAD
                     EEIIKNARTLSTFVFENNFETFDDYLNKLRSSYRRRINKALEYRSNLIINKIQNNEFS
                     EKHYNLYLSIMKRTDNPLETLPIEFFRNYESEIYEFLDKGTLNVIGFIQLKEFDGRLC
                     FLFGGFDKDDVKKYDLYYNMLLKIIEAGIEKKTHIIEFGQTAEESKLKIGCKEEYKYL
                     YVHHSNSILNFIIQKLLPFMSYRPYRTVHHVFKE"
     gene            765..>1436
                     /locus_tag="PLD67_03260"
     CDS             765..>1436
                     /locus_tag="PLD67_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21572.1"
                     /translation="MIVFLEKVRVKTIFSIIDPVRVEPLELCYLKSVLNDMNIDSYIV
                     DELFDFSPPKDITPDIVVLTGYNTAENEIIRMSEVYKNKYPMLKIIAGGVHVQENAEN
                     FRVNTIDYVFHSPSLNTFRLLINKIIDNDGKPLNRGVDTNYKRGWHLGEKESIYEIEN
                     IKPDRDFFYKAGTKLKYLDKKNVALIKGSIGCPYNCSFCYCKLLNNKHFIAADYEKMI
                     EEMESI"
     assembly_gap    1437..1446
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1447..2036
                     /locus_tag="PLD67_03265"
     CDS             <1447..2036
                     /locus_tag="PLD67_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21573.1"
                     /translation="YYWVVDDVLFSTRNDALKFIEIIEKRNSNLNIIGYLRADFICRE
                     KDLLKRLRNAGIREVITGFEATGNEELNEYKKTTDAADYPLAISLLKENNIELTALFM
                     VQPSYGLKEFKNLKKFIKTNKIDLYTISIMTPLKKTKLYEETEELLIDKRPDKYDFLH
                     LVIKPKLPKFIFYTLFYSLHIRLLKSKRVRKFLFN"
     gene            2048..2665
                     /locus_tag="PLD67_03270"
     CDS             2048..2665
                     /locus_tag="PLD67_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT21574.1"
                     /translation="MFNIWNFWADKYDKLWVQKYSLKPTRNYIVKALSKYIKNDGIKV
                     LDLGCGTGELIFELTNKFNNLEVTGIDFSEKMIEISKKRNPSAKHLQMDASELYILDS
                     KYDIIVCTHSMPYYKEPKNVFKHLNNLLSDEGKLLIGFASGDSIYDKFILGFVKLTTG
                     KANYPSDNEFRAMIFPFFEVENLKIIRKKFFMPRIAVYTLKKVKL"
     gene            2662..4023
                     /locus_tag="PLD67_03275"
     CDS             2662..4023
                     /locus_tag="PLD67_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT21575.1"
                     /translation="MKVLLVRPRPHKETIGLQHVMICEPLELEYLISNIPDDIKGETD
                     AEIIDMILEKGSFEEVVYKKKPAFIVFTGYITHVGIIKQMAETAKKIIPSVKTGVGGV
                     HAEVVPEDFESEYIDFVYYKNGIDNFNITLRGLINKKSINEIKEEINIEDKKVIFDYK
                     YPDRNSVKKYRKKYYYMFHNPCALIKTSFGCPYNCSFCFCKEITQGQYFTREMDDIIK
                     ELSSIEEEEVYIVDDDFLYDRNKLESFIAGLKLNNIKKKFLVYGRADFIAQNKDIIAK
                     LKEVGLQAVIVGIESVRAGDLKDYKKGTTVEINEKCIQILQELDIELYATLIIPLDFK
                     KEDFSELVSWLRKLNIKFVNLQPLTPLPGTEIFESYKGSLLADRQRFEVWDMAHVVLK
                     PRYMSIRAFYAEILKAYYKVVMRPKHLGYLIKKYGLKANFKMLAGSTFVSFQYLQKII
                     RSN"
     gene            4023..>5165
                     /locus_tag="PLD67_03280"
     CDS             4023..>5165
                     /locus_tag="PLD67_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT21576.1"
                     /translation="MKKILLIQPSPYDKNHKPIKKNKLYFVGLAMPLLAAMMPEDWEA
                     EIVLEVIEDIPFDTDAELIGISTMGHGVIRSIDIARKFREMGKTVILGGYMASIMADE
                     ALKHCDSVVVGDAELVWNELLKDYDEGRLKKIYKDNLESGFFSTPMPRFDLIINKRIG
                     NFLPVQAGRGCPNTCSFCSVACLYKGHYIKKPLEEVVRDIKQVKELGFKKFLLLDDNI
                     FSDREYLDKLLIEIKKLNMQWMSQCDIRIGRENELLKKLADSGCTTLSFGLESISKES
                     LKGMNKSWVNPLEYTELIQNIHSHGIDVSTEMVVGGEGDTLQSIRDTKRFIEDNKITV
                     PRFYILTPIPGTKFFEDMMKAGRIIKEDIYSFDGTEAVHMPKNMSPD"
BASE COUNT         1957 a          689 c          976 g         1533 t
ORIGIN      
        1 gccattaaaa gcaaaagctc aaattattgg gcttcccata tcggtatctc aaagaggata
       61 ctatggtgat ttaaacaaaa caattgaaat tatcaaaaaa aggaaaggtt taaaaataat
      121 tttaaatgct gatgaagaaa ttattaaaaa tgcaaggact ttatcaacct ttgtgtttga
      181 aaataacttt gaaacatttg acgattattt aaataagctc agaagttctt acagaagacg
      241 tataaataaa gctcttgaat acaggagcaa tttaataatc aataaaattc aaaacaatga
      301 attcagtgaa aaacattaca atttatattt aagcattatg aaaagaactg ataatccatt
      361 ggaaactctt ccgattgaat tttttagaaa ttatgaatct gaaatttatg agtttttgga
      421 taaaggcacc ctaaatgtta ttggatttat acaactgaag gaatttgacg gcaggctgtg
      481 ctttttattt ggaggatttg ataaagacga tgtaaaaaaa tatgatttat attacaatat
      541 gcttttaaag attatcgaag caggtattga aaagaaaact cacattattg aatttgggca
      601 gacagcggaa gaaagtaaat taaaaatcgg ctgcaaggaa gaatataaat atctttatgt
      661 tcatcacagc aattctattt taaattttat aatacaaaaa ttgcttcctt ttatgtcata
      721 cagaccatac aggacagtcc atcatgtttt taaggaatga aattatgata gtatttttag
      781 aaaaagtaag ggttaaaacc atattttcaa tcatagaccc tgtaagggtt gagcccttgg
      841 aattatgcta tttgaagtcg gttttaaatg atatgaatat tgatagctat attgtagacg
      901 agctgtttga ttttagccct ccaaaagaca ttactcctga tattgtagtg cttacgggtt
      961 acaatacggc agaaaatgaa attataagga tgtctgaagt atacaagaat aaatatccca
     1021 tgctaaaaat aatagcaggg ggagttcatg tgcaggaaaa tgcagaaaat ttcagggtaa
     1081 atacaataga ctatgttttt cattcaccaa gcttaaatac atttaggctg ttgattaata
     1141 aaatcatcga taatgacggt aaacctttaa accgtggtgt tgatacaaat tataagagag
     1201 gctggcatct gggagaaaaa gagagtattt acgaaattga gaatattaaa ccggacaggg
     1261 atttttttta taaagccgga acaaaactaa aatatttaga taaaaaaaat gttgctctta
     1321 taaaaggcag cataggatgt ccgtacaatt gctcattttg ttactgcaaa cttcttaaca
     1381 acaagcattt catagctgca gattatgaaa aaatgattga agaaatggaa agtattnnnn
     1441 nnnnnnatta ctattgggta gttgatgatg tattgttttc tacaagaaat gatgctttaa
     1501 aatttattga aattattgaa aaaagaaatt ccaacttaaa tatcatagga tatttaaggg
     1561 cagatttcat atgcagggaa aaggacttgt taaagaggct tagaaatgca ggcattcgag
     1621 aggttattac cggctttgaa gcaacaggaa acgaggagct taatgaatac aaaaaaacaa
     1681 ccgatgctgc ggactatccc ttggcaattt ccttattaaa agaaaacaat attgaattaa
     1741 cagccctttt tatggtgcag cccagctatg gcctaaaaga atttaagaat ttaaaaaagt
     1801 ttataaaaac aaataaaata gatttgtaca ccatttcaat tatgactcct ctaaagaaaa
     1861 caaagcttta tgaagaaacg gaagagttac tgattgataa gagacctgat aagtatgatt
     1921 ttttgcactt ggtaataaaa ccgaaactgc cgaaatttat tttttataca ttgttttaca
     1981 gccttcacat aagacttcta aaatcaaaaa gagtaagaaa atttctgttc aattaagagg
     2041 agagcatatg tttaatatat ggaatttttg ggctgataaa tatgataagc tgtgggtgca
     2101 aaaatattct ttaaaaccca cgagaaatta tatagttaaa gctctttcaa aatatataaa
     2161 aaatgatgga ataaaagtac ttgatttagg gtgcggcact ggagaattaa tttttgaatt
     2221 aacaaataaa tttaataatc ttgaagttac gggaattgat ttttcggaaa aaatgattga
     2281 aatatcaaaa aaaagaaatc cctcagccaa acatttacaa atggatgcat ctgagttata
     2341 tattttggat agcaagtatg acataatagt gtgcacccat tcaatgcctt actacaaaga
     2401 gccaaagaat gttttcaaac acctgaataa tttattaagc gatgaaggaa aattacttat
     2461 tgggtttgca agcggtgaca gcatatatga caaattcata ttaggctttg ttaagcttac
     2521 caccggcaag gccaattatc cctcggataa tgaattcaga gcaatgatat ttcccttttt
     2581 cgaagtggaa aatttaaaaa taatcaggaa gaaatttttt atgcctcgaa tagcagtcta
     2641 cactcttaag aaggtgaaat tatgaaagta ttactcgtaa gaccaagacc tcataaagaa
     2701 acgataggtt tgcagcatgt aatgatatgc gagcctttag agcttgaata tcttatttcg
     2761 aatattcctg atgatatcaa aggtgaaaca gatgctgaaa ttatagatat gatacttgaa
     2821 aaaggaagtt ttgaagaggt tgtatataag aagaaacctg cattcatagt tttcaccggc
     2881 tatataaccc atgtcgggat tataaagcaa atggcagaaa ctgcgaaaaa aattatacct
     2941 tcggttaaga cgggggtagg gggagttcat gcagaagttg tacctgagga ctttgaatcg
     3001 gaatatatag attttgttta ttataaaaac ggaattgata attttaatat aacattaaga
     3061 ggtttaataa ataaaaaaag tattaatgaa ataaaagaag aaataaatat tgaagataaa
     3121 aaagtcatat ttgattacaa atatcctgac agaaattcag taaaaaaata tagaaaaaaa
     3181 tactactata tgtttcacaa cccttgcgcc ttgattaaaa cttcctttgg ctgcccatac
     3241 aactgctcgt tttgtttttg taaagaaata acccaaggtc aatattttac ccgtgaaatg
     3301 gacgatatta taaaagaatt atcctcaatt gaagaagagg aagtatatat agtagatgat
     3361 gattttttat atgatagaaa caaacttgaa agtttcattg caggactaaa gttaaataat
     3421 ataaaaaaga aatttttggt ttacggaaga gctgatttta ttgcacaaaa caaagatatt
     3481 atagcaaaac ttaaggaagt cggcttgcag gccgtaatag tcggaataga atcagtaaga
     3541 gcaggtgatt taaaggatta caagaaggga acaacggttg aaataaacga aaaatgcatt
     3601 caaatattac aggaattaga tattgaacta tatgcaacat tgataattcc ccttgatttt
     3661 aaaaaagaag atttcagtga gttggtaagt tggctcagga aattaaatat aaaatttgtt
     3721 aaccttcagc cgcttacacc tcttcccgga actgaaatat ttgagtctta taaaggctca
     3781 ctcttggcag acagacagag atttgaagtg tgggatatgg cacatgttgt tttaaaacca
     3841 agatatatga gtatcagagc tttttatgca gaaattctaa aagcatatta caaggttgta
     3901 atgagaccga aacatttagg atatttaatc aagaaatacg gcttgaaagc aaattttaaa
     3961 atgcttgcag gctcaacctt tgtaagtttt cagtatttac aaaaaattat caggagtaat
     4021 taatgaaaaa aatattattg atacaaccaa gcccctatga caaaaaccat aaacctatca
     4081 agaaaaacaa attgtatttt gttggacttg ccatgccttt actggcagca atgatgcctg
     4141 aagactggga agctgaaatt gtattagagg taattgaaga tattcctttt gatacggatg
     4201 ccgaattaat cggaatcagc accatgggac acggggttat ccgctcaatt gatatcgcaa
     4261 ggaaatttcg cgaaatggga aagaccgtaa tactgggagg ctacatggca agcatcatgg
     4321 ctgatgaagc attgaagcac tgcgacagtg ttgtagtggg agatgcagaa ttggtttgga
     4381 atgagctatt aaaagattac gatgaaggaa gactcaagaa aatatataag gataatttgg
     4441 aaagcggctt tttttcaact ccaatgcctc gatttgacct tataataaat aaaagaatcg
     4501 gtaattttct gcctgttcag gcaggaaggg gctgtcccaa cacatgcagc ttctgttctg
     4561 ttgcatgcct gtataaaggt cattatataa aaaagcctct tgaagaggta gttagagata
     4621 ttaagcaggt aaaagagtta ggatttaaaa agtttctttt gttggatgac aatatttttt
     4681 ctgacagaga atatttggac aaactgttga ttgaaattaa aaaattaaat atgcagtgga
     4741 tgagccagtg tgatattcgt ataggcagag agaatgaact gttaaaaaaa ctggctgaca
     4801 gcggatgtac aacattaagt tttggacttg aaagtatttc aaaggaaagt ttaaagggga
     4861 tgaacaaatc ctgggtaaat cctttggaat atactgagct tatacaaaac atacattctc
     4921 acggtataga cgtttcaaca gaaatggttg taggtggcga gggagacaca ctgcaatcca
     4981 taagggatac aaagagattt attgaagata acaaaatcac tgtgccgcgg ttctatatat
     5041 taactcctat tccaggaact aaattttttg aggatatgat gaaagccgga aggattatca
     5101 aagaagacat ttattccttt gatggtactg aagcagtgca tatgcctaag aatatgagtc
     5161 ccgac
//
LOCUS       DAOSOW010000085         5160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_16230_length_5160_cov_3.925171, whole genome shotgun sequence.
ACCESSION   DAOSOW010000085 DAOSOW010000000
VERSION     DAOSOW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5160
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16230_length_5160_cov_3.925171"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2356)
                     /locus_tag="PLD67_03285"
     CDS             complement(<1..2356)
                     /locus_tag="PLD67_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOT21577.1"
                     /translation="MYYRKSITETCEELNTAQSTGLTQEEAKKRLLSDGPNALVEKKA
                     KTKLQMFLAQLKDTLIYILFAAAAISIALNEITDAIIILLVVLLNAVIGMVQESKAEA
                     ALEALKNLSSPYAMVRREGRVMEIPASELVVGDLVILEAGRIIPADLRLIQSVNLKIE
                     ESALTGESVPVDKNADMVADKEVGIGDRLNMAFSSTSVSYGRGEGVVVYTGMDTEIGK
                     IAAMISESTEELTPLQKRLNDLGKVLGIVAVVIVVAMFGIAVLQGRDIIEMFITAIAL
                     AVAAVPEGLTAVVTIVLALGVTKMVRVNTIVRKLPAVETLGAVSIVCSDKTGTLTQNK
                     MTVTKVYLDGEIKNTDDLSYENNKIFLDGFILCNDASIANNTRIGDPTELALLDMGDK
                     INLSREGLEKTNPRINEQSFDSARKLMTTVHRDQDGKVMSYTKGAMDILLNRCNKIYI
                     KGETKDITNEHIEEINRAATEMAKGALRVLALGIKEDDDSASEEDLTFVGLVGMIDPP
                     RPEAKDSVKTLRSAGIRTIMITGDHKDTALAIAKELGIAEDESQCITGSELNEMTQEQ
                     LNEKVCDIRVFARVSPEHKVMIVKAFKSNGSIVSMTGDGVNDAPSLKSADIGVAMGIT
                     GTDVAKGASDMVLTDDNFATIEKAVAEGRGIYNNIKKTVLFLLSSNFGEVISMFSSIA
                     AGLIAPLQSIHILWVNLITDSLPALALGVDPKSKEIMNEKPRDPNESLFAKGGLIFTV
                     YNGIMIASLTLAAFLWSPVIHLNEAGIPVTLGNIRDLFNGVLSIP"
     gene            2627..2995
                     /locus_tag="PLD67_03290"
     CDS             2627..2995
                     /locus_tag="PLD67_03290"
                     /inference="COORDINATES: protein motif:HMM:NF024082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HOT21578.1"
                     /translation="MKLGKLFKYSLYLFILSLIIYFILTVYIYYFSNNPGFEIVYSFI
                     VPICIFAVSMLYSRSVHEKGLLRGIEIWIVYFAMVLLIKVLFRYPSEIRILYNGVILI
                     MSILGGIIGVNMKNKSIKNK"
     gene            3073..3213
                     /gene="scfA"
                     /locus_tag="PLD67_03295"
     CDS             3073..3213
                     /gene="scfA"
                     /locus_tag="PLD67_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004625848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HOT21579.1"
                     /translation="MKYIKTLNNYKLTDNLSAKGCGECQTSCQSACKTSCTVGNQKCE
                     KE"
     gene            3354..4688
                     /gene="scfB"
                     /locus_tag="PLD67_03300"
     CDS             3354..4688
                     /gene="scfB"
                     /locus_tag="PLD67_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HOT21580.1"
                     /translation="MIHVFNIKDLYFATDTSTGLVHAVDKIVFDLLMNEKFKDENKYS
                     ELYNEYGESTVREALSEIQYLIDNKMLYTEDIKYVNTIKPVIKAMCLNMTHDCNLRCE
                     YCFASQGTYNGEKAFLSFETGKKAFDYLVKNSGNRKNLEVDFFGGEPLMNFDVIKKLV
                     DYGRSLEKEYNKHFRFTVTTNGVLLDEEKMDYINENMDNVVLSIDGRKETNDRMRKTI
                     NKKGSYDLIVDNYKKFISKRGSKDYFARGTYTSNNLDFSEDVKHMRELGFDKISVEPV
                     VAKDEKKYALKKEHVDILKKEYEKLAEYYIESYKSKDRRFQFFHFNIELEGGPCIYKR
                     SIGCGAGTEYVAVTPSGDLYPCHQFVGNEEFIIGNVEEGITNRALADKFKNVSVNDKP
                     ACRDCWAKYFCSGGCHANAYNFNKDFTVPYDVGCELEKKRIECAIYLKAKIN"
     gene            4806..>5160
                     /locus_tag="PLD67_03305"
     CDS             4806..>5160
                     /locus_tag="PLD67_03305"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HOT21581.1"
                     /translation="MQIGFIVILIIAIFVAIFAIQNGTPVPVDLFFARYEMPLAVIMM
                     ICLILGAVIVLILGTTRQFKKRSEHNELKNKLKTLEGEKAKHEIDMKALESEKAQFQT
                     NIEALETEKQRLQNEI"
BASE COUNT         1668 a          889 c          931 g         1672 t
ORIGIN      
        1 cgggtatact gagaacaccg ttaaataaat cacgtatatt tcccaaggtt acaggtattc
       61 ctgcttcatt taaatgaatg acgggtgacc ataaaaaggc tgcaagagtc aatgatgcaa
      121 tcattattcc gttgtaaacg gtaaatatca aacctccctt agcaaacaag ctttcatttg
      181 ggtctcttgg tttttcattc attatttctt tgctcttcgg gtcaactcct aatgctaatg
      241 caggaagcga gtcggttatc aggttaaccc aaagaatgtg tatggactgc agcggtgcta
      301 tcagtcctgc cgcaatagat gaaaacattg atataacttc accaaaattg gaggataaca
      361 agaatagaac cgtcttttta atattattgt aaatacctct tccttccgca actgcctttt
      421 caatggttgc aaaattatca tccgtaagaa ccatatcaga agcacctttt gctacgtcag
      481 tacctgttat gcccatggca acaccaatat cagctgattt taatgatgga gcgtcgttaa
      541 caccgtcacc ggtcatagaa actatgcttc cgtttgattt aaatgctttt actatcatta
      601 ccttgtgttc cggtgaaact cttgcaaaaa ctcttatatc acaaactttt tcatttaatt
      661 gctcctgagt catctcattc aattcactgc cggttatgca ttgtgattca tcttctgcaa
      721 taccgagctc cttggcaata gcaagagcag tatccttgtg gtctcctgta atcattatag
      781 tcctgatacc tgcacttctt aaagttttta ccgaatcctt tgcttcgggt cgaggagggt
      841 ctatcattcc taccaagccc acaaatgtga gatcttcttc gcttgctgaa tcgtcatctt
      901 ctttgattcc aagagccaat actctaagag ctccttttgc catttctgtg gcagccctgt
      961 ttatttcttc aatgtgttca tttgttatat cttttgtttc gccctttata taaattttat
     1021 tgcatctgtt caaaagtatg tccatggcac ccttagtgta gctcattacc ttgccgtctt
     1081 ggtccctatg aactgttgtc atcaattttc ttgcagaatc aaaggattgc tcattgattc
     1141 tcggattagt tttttctaat ccttcccttg acaaattaat tttgtctccc atatccaaga
     1201 gagctaactc tgtagggtct cctatacgag tgttattagc tatggaagca tcattgcaaa
     1261 gaataaagcc gtccaaaaat attttgttgt tttcataaga taagtcatcg gtatttttaa
     1321 tttcaccgtc taaatatact tttgttacgg tcattttatt ttgagtgaga gtaccggttt
     1381 tgtctgagca tacaatgctg actgctccta acgtttcaac tgcgggaagc ttgcggacta
     1441 tggtatttac ccttaccatt tttgtaacac caagagccaa tacgattgtt acaactgcag
     1501 taagcccttc aggcacggca gctacggcta aggcaatggc tgttataaac atttcgatta
     1561 tgtctcttcc ctgcagaaca gctattccaa acatggctac aactataact acggcaacta
     1621 tacctaatac ttttcccaag tcattgagtc ttttttgaag aggagttaat tcttcggtgc
     1681 tttcacttat cattgcagct atttttccta tttcggtatc cattccggta taaactacaa
     1741 caccttcacc acggccgtat gaaacacttg tggaagagaa tgccatattt aggcggtctc
     1801 cgattcctac ttccttgtca gccaccatat cggcattttt gtctacgggt acggattctc
     1861 cggttaaagc agattcttcg atttttaagt ttaccgactg aataagtctt aaatcggcag
     1921 gaataattcg tccggcttcg agtattacta aatctcccac aaccagctca cttgcaggta
     1981 tttccattac cctgccctca cgccttacca ttgcataggg gcttgaaaga ttcttcaagg
     2041 cttcaagtgc agcttctgct tttgattctt gaaccatacc tattactgca ttcaataaaa
     2101 caactaatag aattattatt gcatctgtta tttcgtttag agctatggat attgctgcag
     2161 ctgcaaacaa tatgtaaatc agtgtatctt ttagttgggc caagaacatc tgcagctttg
     2221 tttttgcctt tttctcaact aatgcattgg gaccgtctga caacagcctc tttttcgctt
     2281 cttcttgggt taaaccggta ctttgagcgg tgtttaattc ttcgcatgtt tctgttatgc
     2341 tttttcggta atacattgtc tactccttta agattattca caactgttta ataaaaaaga
     2401 cttttagcat acctactcca gatataccaa aagtctcgtt tcctaagttg cccgaggatt
     2461 ttcagccaga atacatatat tccgtgttgt tgactgaaaa tttcaaacta ctccctttta
     2521 gcaagataac tttatcaaat atagaaatct ttgtcaagta atatattaat gttctttttt
     2581 attatcggat gaatattatt tagtaagtca tgcttaagga ggtattatga agttaggtaa
     2641 attgtttaag tattctttgt atttatttat tttatcttta ataatttatt ttatactgac
     2701 cgtttatata tattattttt caaataatcc cggttttgaa atagtctaca gcttcattgt
     2761 tccaatatgc atatttgcgg tttcaatgtt gtattcaaga agtgttcatg aaaaagggct
     2821 gttaagggga attgaaattt ggatagtata ttttgcaatg gtcttattga taaaagtctt
     2881 attcagatac ccgtccgaaa taagaatact atacaacggg gtgattctta taatgtcaat
     2941 actgggagga atcatcggtg ttaacatgaa aaacaagagt ataaaaaaca aatagtaata
     3001 aaaaaataaa aaaaatactt taaaataacc tcaactaatg ctataatcta ttaagtttat
     3061 aaggaggttg tcatgaaata cataaaaaca cttaataatt ataagttaac ggataattta
     3121 agcgcaaagg gatgcggaga atgccagaca tcatgtcagt cagcttgcaa aacatcttgt
     3181 actgtcggaa atcaaaaatg tgaaaaagaa taattgtagt atagtttatt gtttagacag
     3241 agtttgtcat tctgaaggcg cagcttgaga ggtgctctaa caattagagg cagcacacta
     3301 catagcttcg ttcaggatga cttattttta attagcaaaa tttcggagga agtatgatac
     3361 atgtatttaa tataaaagat ttatatttcg caacagatac aagcaccgga ctggtacatg
     3421 ccgtcgataa gatagtattt gatttattaa tgaatgaaaa attcaaggat gaaaataaat
     3481 acagtgagtt atacaatgaa tacggagaaa gcacagttag agaagcttta tcggaaatac
     3541 aatatttaat agacaataaa atgctctata ccgaagatat taaatatgta aatacaataa
     3601 agccggttat taaagcaatg tgtttgaata tgacccacga ctgcaattta agatgcgaat
     3661 actgctttgc ttcacaaggg acatataacg gagaaaaggc atttctaagt tttgaaacag
     3721 ggaaaaaggc atttgattac ttggtgaaaa actccggtaa cagaaaaaac ttagaagtag
     3781 attttttcgg cggagaacct ctcatgaatt ttgacgtaat caaaaaactg gttgattacg
     3841 gaagaagctt ggagaaagaa tataacaaac atttcagatt taccgttaca accaacggtg
     3901 ttcttttaga tgaagaaaaa atggactata ttaatgaaaa tatggacaat gtggttttaa
     3961 gcatcgacgg aagaaaagaa acaaatgaca gaatgcgtaa aacaataaat aaaaaaggaa
     4021 gctatgactt aatagttgat aattataaga aatttatcag caaaagaggc agtaaggatt
     4081 attttgccag gggaacatac acttccaata atttagactt ctcggaagat gtcaagcata
     4141 tgagagaatt aggttttgat aaaatttctg tagagcccgt tgttgctaag gatgagaaaa
     4201 aatatgcact aaaaaaagag catgtggata ttttaaaaaa agaatatgaa aaattagctg
     4261 aatattatat tgaatcatat aaaagtaaag atagaaggtt tcagtttttt cattttaata
     4321 tcgaactgga gggaggtccc tgcatttaca aaagatccat tggctgcgga gcaggaactg
     4381 aatatgttgc cgtaaccccg tcaggtgatt tgtatccttg tcatcaattt gtaggtaatg
     4441 aagaattcat tatcggtaat gttgaggaag gtattacaaa tcgggctctt gccgataaat
     4501 ttaaaaatgt atcggtaaat gataaacctg cgtgcagaga ttgttgggca aaatactttt
     4561 gcagcggtgg ttgtcatgcc aatgcatata attttaacaa ggattttact gtcccatatg
     4621 atgttggatg cgagttagaa aaaaaacgaa tagagtgcgc aatttattta aaagctaaaa
     4681 ttaattaaga ctctccttca attaaaggtt gtccccatac ctgtaaaaaa aacagtggta
     4741 attgtatatg gtaatatagt ataatacaga tataataaaa ataaagacaa gagaggagta
     4801 ttaatatgca aattggtttt atagtaattc ttataattgc gatttttgtt gcgatttttg
     4861 caatacaaaa tggaacaccg gttcctgttg atttattttt tgcaagatat gaaatgccct
     4921 tagctgtaat catgatgatt tgtctgattc ttggtgccgt gatagtctta atattaggta
     4981 cgaccaggca atttaaaaaa agatccgagc ataatgaatt aaagaacaag ctcaaaactc
     5041 ttgaaggcga aaaagctaag catgaaatag atatgaaagc attagaatca gaaaaagctc
     5101 aatttcaaac aaatatagag gctcttgaaa cagaaaaaca aaggcttcag aatgaaatag
//
LOCUS       DAOSOW010000086         5129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_16385_length_5129_cov_4.624557, whole genome shotgun sequence.
ACCESSION   DAOSOW010000086 DAOSOW010000000
VERSION     DAOSOW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16385_length_5129_cov_4.624557"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1147
                     /locus_tag="PLD67_03310"
     CDS             158..1147
                     /locus_tag="PLD67_03310"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007058915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOT21582.1"
                     /translation="MKYIENNSTDPHYNLAFEEYVFKNLDNNEDFVLLWRNGPSIIVG
                     KNQNTIEEINMEFVKNNNINVVRRVTGGGAVYHDLGNLNFSFITKAQSNLIDFKTYNI
                     PIVKSLEKLGVDCELSGRNDIVIDGKKFSGIAQSIIKGRVLNHGTLLFDSKLDILSKA
                     LNVKRDKIESKGVKSVSSRVTNIKPYVKDDIDVLQFKQILLKNIFEYFNQPVEIFELS
                     DEDRNKIQKMVDERYGTWEWNFGRSPEFNYKGYDRFEGGGVEVRLHVCNGIIESCKIY
                     GDFFGKGDVKNLEDKLLGVKYDINEINKAIEDISIDEYLGRITKEEFLRCLFS"
     gene            complement(1191..1643)
                     /locus_tag="PLD67_03315"
     CDS             complement(1191..1643)
                     /locus_tag="PLD67_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT21583.1"
                     /translation="MKKILIPIDGSPLSVKIGEKAVELGKLIDAELIFITVINMPSED
                     KYAYFGMTVENQFSANRKEMLNKLMQQESKMLNIIVRNLDTGNLKVTQKLLVGRPAAE
                     ILKFAEEENINYIFMGRRGFSPVQRFFVGSVTQKVISAAPCPVIVING"
     gene            complement(1660..2100)
                     /locus_tag="PLD67_03320"
     CDS             complement(1660..2100)
                     /locus_tag="PLD67_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT21584.1"
                     /translation="MKKILVPVDGSAASQKAVAQAFDIGRKYNSDVVVFHVFIEHDID
                     MYNKFGVVIDPDMNKLKKRLKENESKMLDSIINQYDNTGINLKKKIVSGVAYEEILKE
                     AKDGNYDLIVIGQRGYSKIKRFFVGSQTVRVISDATCPVLVVHE"
     gene            2269..4476
                     /locus_tag="PLD67_03325"
     CDS             2269..4476
                     /locus_tag="PLD67_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOT21585.1"
                     /translation="MEILNSLNDKQREAASHDKGPILVIAGAGTGKTRVLTHRTAYLI
                     ESGKAMPWEVLAITFTNKAASEMKERIGRLIDYSIDRMWIGTFHSVCVRILRSFIDRI
                     GYDKNFIIYDDDDQKIVIKDCIKELELDPKLYNYNSIKALISNEKNRRNTPDNYIKDN
                     YLNYANRNKGEVYALYEKKLKAANALDFDDLILKTLELFEKNEDILKHYQERFKYILV
                     DEYQDTNNAQYNLIRILGKKKNGESNVFVVGDEDQSIYGWRGADISNILNFENDFKGA
                     KIVKLEKNYRSTDVILNAANGVIKNNYQRKGKNLYTEQKKGNLIRIIETDNERDEAFT
                     TAALIRKEHRENNINYSEIAILYRTNAQSRALEEGLIREGIPYKIVGGVKFYDRKEIK
                     DIMAYLRLILNQKDDVSFERIINVPRRKLGQKSTDTISNYAEANGVSKFEACFDFDKM
                     GLIPSAAKSIESFVSMMEMLMIKKDAMPLSEFIISVIEDTGLKDMLLQDETVEGRSRV
                     ENIDEFLSAAKDFEERYEENTLEDFLAHISLLADIDKTDSIKDVVTLMTVHSAKGLEF
                     DTVFITGLEDGMFPSIHFDGEDNVEEERRLFYVAITRAKRMLYITHARDRIRFGSHEM
                     RMKSRFLEEIPEECIEESDPYSYANKKLEQLESQIARKTSFFEYRAEITEKEKPKIKS
                     DKFEPGDKIMHKSWGKGTVVQIKDSGDDKIIVAAFENKGIRNLSLKLAPIEKI"
     gene            4491..>5129
                     /locus_tag="PLD67_03330"
     CDS             4491..>5129
                     /locus_tag="PLD67_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOT21586.1"
                     /translation="MDKKLLLMKEKIRLLNEANKAYYQEDREIMSNYEYDKLYDELKK
                     MEEETGIVLSNSPTIRVGYELLSNLPEERHEKVMLSLDKTKEVTALKDWLGEKTGALS
                     WKLDGLTIVMTYEGGRLVKAVTRGNGEVGEVVTNNAKVFTNIPLSIAYKGSLVLRGEA
                     VISYSDFYKINETIPEAAGKYKNPRNLCSGSVRQLNNRITAERNVGFYAFSVV"
BASE COUNT         1908 a          756 c          983 g         1482 t
ORIGIN      
        1 tcattatgtc accaccaaaa ttagtataat acaaaattag acaaaatcca actctgaaaa
       61 tgtaccttaa actaaatatt gaattatcat aatctttatt atataatttg caaaataaga
      121 tttacataat ttcaattatg ttaacaagga gtgcagcatg aagtatatcg aaaataattc
      181 gacagaccca cattataatt tagcatttga agagtatgtt tttaaaaatt tagataacaa
      241 tgaagatttc gttttgttat ggagaaacgg accttccata attgtaggta aaaatcaaaa
      301 tactatagaa gaaattaata tggaatttgt taagaacaac aatataaatg ttgtcagaag
      361 agtaaccggc ggcggtgctg tttatcatga tttggggaat ttaaattttt cttttataac
      421 taaggcacaa agcaatttaa tagactttaa aacatacaat attcccatag tcaagtcatt
      481 ggaaaagttg ggagttgact gtgaattatc gggaagaaat gatattgtta ttgacggaaa
      541 gaaattttca ggcatagcac aaagcattat aaaaggaaga gtattgaatc acggaaccct
      601 gctttttgat tcaaaactgg acatattgtc taaagcatta aatgtaaaaa gagataagat
      661 agaatctaag ggagtaaagt ccgtatccag cagagtgaca aatattaaac catatgtcaa
      721 ggatgatatt gatgtgctgc aattcaaaca gattcttcta aagaatatct ttgaatactt
      781 caatcaacct gttgaaatat ttgaattatc cgatgaagac agaaataaga tacaaaaaat
      841 ggttgatgaa agatacggaa cttgggaatg gaactttgga aggtccccgg aattcaacta
      901 taaaggttac gacagatttg aaggaggagg agtagaagtt agacttcatg tgtgcaatgg
      961 aattattgaa agctgtaaaa tctatggtga ttttttcggc aagggagatg ttaagaattt
     1021 agaagataag ctattaggtg ttaagtatga tatcaatgaa ataaataaag ctatagaaga
     1081 tatttcaatt gatgaatatc ttggcaggat cacaaaggaa gaattcctgc gatgtctatt
     1141 cagctaattt taaagtacgg ggacaaggta tgtcccccaa cccttcctaa ttatccgttt
     1201 attactatta ccggacatgg tgccgctgaa ataacttttt gagttacaga gcctacaaaa
     1261 aatctttgaa ccggagagaa ccctcttctt cccataaaaa tataattaat attttcttct
     1321 tctgcaaatt ttaaaatttc agccgcaggc ctgcctacca ataatttttg agttaccttt
     1381 aaattaccgg tatctaaatt tcttacaata atattgagca ttttagattc ctgctgcatt
     1441 aatttattaa gcatttcctt gcgatttgct gaaaattgat tctcaacggt cataccgaag
     1501 tatgcatatt tgtcttctga gggcatgtta ataacagtta tgaaaattaa ttcggcatca
     1561 attaatttgc ctaattctac ggctttttca ccaattttta cagataaggg cgagccgtcg
     1621 ataggtatta aaattttttt cataataacc tccaattatt tattcatgaa ccactaagac
     1681 agggcaagtt gcatcagata tgacacgaac tgtttgagag ccgacaaaga agcgtttaat
     1741 ctttgaatat cctctttgac cgataactat caaatcataa ttaccatctt ttgcttcttt
     1801 taaaatttct tcataagcca caccggaaac gatttttttc tttaggttaa tacctgtgtt
     1861 gtcatactga ttaattattg aatccagcat tttactttca ttttctttca atctcttttt
     1921 tagtttgttc atatcaggat ctattacaac accaaactta ttgtacatat caatgtcatg
     1981 ttcaatgaaa acatgaaaaa ccacaacatc actgttatat tttcttccaa tatcaaaagc
     2041 ttgggcaaca gctttctgag aagcggctga gccatccacg ggtaccaata tttttttcat
     2101 aaagtccctc cttagccaat aattagaata ttaactaata tacttatacc taaatttatt
     2161 taccataaac aaatatattc ctaaaacaat aatcagaatc ttgagagtaa tggttgactg
     2221 tgatataatt aaaaaaaatg tccccgtact ttttcagata ggagaaaaat ggaaatacta
     2281 aattcattaa atgacaaaca aagagaagca gcttctcatg acaaaggtcc catattggtt
     2341 attgcagggg caggcaccgg gaagacaaga gtcctgactc accggacggc atatttgatt
     2401 gagtccggaa aagcaatgcc ttgggaagtt cttgcaataa catttaccaa taaagcagca
     2461 agtgaaatga aggaaagaat aggtcgtctt attgattatt ccatagacag aatgtggatt
     2521 ggaacttttc actctgtttg tgtacgcata ttaagaagtt ttattgacag aataggttat
     2581 gataaaaact ttattattta cgatgatgat gaccaaaaaa tagtaataaa ggactgcatt
     2641 aaggaattgg aattagaccc caagttatat aattataaca gtattaaggc acttataagc
     2701 aatgaaaaaa acaggcgcaa tactcccgac aactatatta aagataatta cttaaattat
     2761 gcaaacagga ataagggaga agtatatgct ctttacgaaa aaaagcttaa agctgccaat
     2821 gcattggatt tcgatgattt aattctaaaa acacttgaac tgtttgaaaa aaatgaagat
     2881 attttaaagc actaccaaga aaggtttaag tatatacttg tggatgaata ccaggatacc
     2941 aataatgctc agtataatct cataagaata ttaggtaaaa agaaaaacgg tgaaagcaat
     3001 gtatttgtgg taggagatga ggaccagtcc atttacggct ggaggggtgc cgatataagc
     3061 aatattctta attttgaaaa tgacttcaaa ggagccaaaa tagtcaagct tgaaaaaaat
     3121 taccgctcta cggatgtaat tttaaatgct gcaaacggag ttattaaaaa caattatcaa
     3181 cgaaagggta aaaacctgta tacagagcaa aaaaagggaa acttaatacg aataatagaa
     3241 acagacaatg aaagagatga ggcttttacc acggcagcat taattcgcaa agagcatagg
     3301 gaaaataata tcaattactc tgaaattgca attttataca ggacgaatgc acagtcaaga
     3361 gccttggaag aaggtttaat tagagaaggg ataccctaca aaattgtagg aggagtaaaa
     3421 ttttacgata gaaaagaaat taaggatata atggcttatc taaggcttat cctaaatcaa
     3481 aaggatgacg tgagttttga aaggataatc aatgtaccga gaagaaaatt aggacaaaaa
     3541 tcaaccgata ccatttcaaa ttatgctgaa gcaaatggag tttcgaaatt tgaagcatgt
     3601 tttgactttg ataagatggg gcttatacct tcagcagcaa agagtattga aagctttgtg
     3661 tccatgatgg aaatgctgat gattaaaaag gatgcaatgc ctttaagcga attcataata
     3721 agcgtaatcg aagatacagg gctgaaagat atgctacttc aagacgaaac ggttgaagga
     3781 agaagtcgtg ttgaaaatat agatgaattc ttgtccgcag caaaggattt tgaagaaaga
     3841 tatgaggaaa atacattaga agattttttg gctcatattt cattgcttgc cgatatagat
     3901 aagactgatt ctataaaaga cgttgtaaca ttgatgactg tacacagtgc taagggtctt
     3961 gaatttgaca cggttttcat aacaggtctt gaagacggca tgtttccgag cattcatttc
     4021 gatggtgaag acaatgttga agaagagaga agactcttct atgttgctat aacaagagcc
     4081 aaaagaatgc tctatattac tcatgcaagg gacagaattc gttttggaag ccatgaaatg
     4141 aggatgaaat caaggttttt ggaagagatt cccgaagaat gcatagaaga atccgaccct
     4201 tattcttatg ccaataaaaa acttgagcaa ttggagtccc aaatcgcaag aaaaaccagt
     4261 ttttttgaat atagagctga aatcacggaa aaagaaaaac caaagattaa atcagataag
     4321 tttgagcccg gtgataaaat aatgcataag tcttggggga agggaacagt agtacaaata
     4381 aaggacagcg gagacgataa aataatcgtg gcagcttttg aaaataaggg gataaggaat
     4441 ctttcactca aacttgctcc aatcgaaaaa atttaaacgg aaggtaaagt atggataaga
     4501 aactcctttt aatgaaagaa aaaataaggc ttttaaatga agcaaacaaa gcatattatc
     4561 aagaagacag agaaataatg tcaaattatg aatatgacaa gctttacgat gagcttaaaa
     4621 aaatggaaga agaaacaggg atagtactgt ctaacagccc caccatccgt gttggatatg
     4681 agctcttatc aaatttgcct gaggaaaggc atgagaaggt tatgctttca ttggataaaa
     4741 caaaggaagt tactgcttta aaggattggc tcggagaaaa aacaggagcc ctttcgtgga
     4801 agctggatgg ccttacaatt gtgatgactt atgaaggagg aaggcttgta aaggctgtaa
     4861 ccaggggtaa tggagaggtt ggagaagtag ttaccaacaa tgcaaaggta tttactaata
     4921 ttcctcttag tattgcctat aaaggaagtt tggttttacg aggagaagca gttatcagtt
     4981 attcagactt ttataaaata aatgagacaa ttccggaagc tgcgggtaaa tataaaaatc
     5041 ccagaaatct ttgcagcggc tccgtaaggc agctaaacaa tagaataacc gcagaaagaa
     5101 atgtggggtt ttatgcattt tcggtagtt
//
LOCUS       DAOSOW010000087         5121 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_16435_length_5121_cov_3.669957, whole genome shotgun sequence.
ACCESSION   DAOSOW010000087 DAOSOW010000000
VERSION     DAOSOW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16435_length_5121_cov_3.669957"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLD67_03335"
     CDS             <1..194
                     /locus_tag="PLD67_03335"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOT21587.1"
                     /translation="IAFFIIAVYYGVIYMMKQGAQSSPALGIRMNLMYMAIPLGCGVM
                     LLHALNAIFEILVTGEVAE"
     gene            194..1456
                     /locus_tag="PLD67_03340"
     CDS             194..1456
                     /locus_tag="PLD67_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015553024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOT21588.1"
                     /translation="MGSILLLTFLGCLALGVPIAFSVAVAGVVVLIVGDVNLLIVVQR
                     LFASSDSFSLVAIPFFILAGDLLARGEMSKMLVAFAEACIGKVKGGLSAVSVLASMFF
                     AAISGSGAATTAAVGTSMLPELEKRGYEVDRAAALIASGGCIGVVIPPSVPMVLYAVI
                     AEQSVTRLFKNGFFPGFLMGGILIAISLYEAKKFNYPDGRAFEFKYMVETFRKAVWAL
                     IMPLIILGGIFSGYFTPSEAAGVAVVYSMFASMFIYKDLTLKELGQIMLGSGKTSAII
                     MTIIACSGVFGWALANWQIPEAVAQAVLSVSDNKYIILLLMAVIVLIAGVFMETSSAI
                     ILITPVFLPLVRMLNIDLIHFGLIFTIGIAIGMITPPVAIDLFVASSITGMPIERIAK
                     KVVPYLIGLIIVFFIYIYLPLFIPSLIL"
     assembly_gap    1457..1556
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1631..2089
                     /locus_tag="PLD67_03345"
     CDS             1631..2089
                     /locus_tag="PLD67_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HOT21589.1"
                     /translation="MRIIIDGDACPQKVREICEKAAKDFGVGLIIVSDIDHYIVSDFE
                     VIVVEQGRDSVDYKIVQIFQQGDILITQDYGLASLLLGKASAIIHTAGFFINKNNIDS
                     LLQSRYISDKIRKQGGKSKGPSKRRKEQDENFKKCLYKVLREYMSNKKGR"
     gene            complement(2147..3244)
                     /locus_tag="PLD67_03350"
     CDS             complement(2147..3244)
                     /locus_tag="PLD67_03350"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT21590.1"
                     /translation="MDKNILITTAPFGNGHKMVATAIKNAFIKKGYNNVVVVDLFTET
                     HPKITEAIKKAYIRSYDFGKAYSKLYYGSEKLTDKKVVELYRNFGFSKLKDISEVFKP
                     DVIINTFPILASLKLKNEQGENIPVFNIVTDYYAHKLWISKEIDKFYIATYELQEEFK
                     KMNIPPEKIVVSGIPIRDAFEEIDNISKLHAKYKFNRRKRIILINSGAFGVLKDIKKV
                     CTELCKNKNIQVAVVCGNNIYQKTKLESLRLRNLKVFGFVEDIHELYKIACCMITKPG
                     GITLTEALSVQLPLIIIKPVPGQEKENARYFEKKGAAIIANNTYQIINSAIDLINNPE
                     LLSAMRKNMKKMYNKSSSDRIVDDVLGSLEE"
     gene            3349..3900
                     /locus_tag="PLD67_03355"
     CDS             3349..3900
                     /locus_tag="PLD67_03355"
                     /inference="COORDINATES: protein motif:HMM:NF013346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOT21591.1"
                     /translation="MYKEFLETFILVFVAEMGDKTQLMLMTLAAKYKIIDVLLGIFLG
                     VLLNHGTAVFIGSCISNLIDEYLLHVFAGVVFIIFGIITIVFEDEDENKKQYGLGPVI
                     TTALTFFLGEMGDKTQLTCMTLSMDAYYPSAVLAGSVTAMLAIGLAGIIIGTSLTKFI
                     PSYIIKTISGLIFIIFGVVRMII"
     gene            3901..4545
                     /locus_tag="PLD67_03360"
     CDS             3901..4545
                     /locus_tag="PLD67_03360"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019107878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOT21592.1"
                     /translation="MNLKNIKDKLKNIKPKPIDFDVSFAVLVPIIEIDGELNLIFEVR
                     SNSITQPGEISFPGGRIEEGESPRKAAIRETSEELLLNKENIEIISELNYASSKSGAF
                     VFTFLGLIKDVDPDEIEYSIDEVSEVFFVPLSFFLENRPEKYYMNYYPKADKDFPYHM
                     VNNGEDYNWGNIRYPVYFYKYYNYIIWGLTAKITYSLIKELTNSDVKKRNNNES"
     gene            4535..>5121
                     /locus_tag="PLD67_03365"
     CDS             4535..>5121
                     /locus_tag="PLD67_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOT21593.1"
                     /translation="MKASIFNIQKFSIHDGPGIRTTVFFKGCPLQCIWCHNPESQNLG
                     KEILYDKNKCTLCGSCIKICQNNAIELKDNDLEINMDKCTFCGDCTVCCINSAKQIAG
                     KEYTVDEVMKEVLKDRVFYKNSKGGVTLSGGEPLIYAAFVEELLMELKKENIHTAVDT
                     CGAVDFKVLERISKYTDLFLYDIKSMDEEKHILYTG"
BASE COUNT         1580 a          809 c          976 g         1656 t
ORIGIN      
        1 gcattgcatt tttcattatt gcggtttatt atggcgtaat atacatgatg aaacaaggag
       61 cgcaaagctc acctgcctta ggcataagaa tgaatttaat gtatatggca attccactcg
      121 gatgcggagt aatgctgctt cacgccctaa atgcaatttt tgaaatttta gttacagggg
      181 aggtggcaga ataatgggta gtatactact tttaaccttt ttaggatgtc ttgccttagg
      241 tgttcccatt gcattttctg ttgctgttgc cggtgtagtc gtactgatag taggtgatgt
      301 aaacctattg atagtagtac agcgcttatt tgcttcctca gattcatttt cattggttgc
      361 aattcccttc tttattttgg caggggattt gcttgccaga ggagaaatgt caaaaatgct
      421 ggttgcattt gcagaagcat gtataggcaa agttaaagga ggactgtcgg ctgtttctgt
      481 attggcatca atgttctttg ctgctatatc cggctcaggc gctgctacca ctgcagcagt
      541 tggaacatct atgctgcctg aacttgagaa aagagggtat gaggtagatc gtgcggccgc
      601 tttaatagct tcgggaggat gtatcggagt agtcattcct ccgtctgttc ccatggttct
      661 atatgctgta atagccgagc aaagtgtaac cagattattc aaaaatggtt tttttcccgg
      721 cttcctgatg gggggtatat taatagcaat ttctctttat gaggctaaaa aattcaacta
      781 tccggacgga agggcatttg aattcaaata tatggttgaa actttcagga aagccgtttg
      841 ggcattgata atgccgctta ttatactagg cgggatattt tcgggatatt ttactccatc
      901 tgaagccgcc ggtgttgcag ttgtatattc catgtttgcg tcaatgttta tttacaagga
      961 tttgactctt aaagagcttg gacagattat gcttggtagc ggcaagacct ctgcaatcat
     1021 tatgaccata atagcatgca gcggtgtttt cggatgggct cttgccaact ggcagatacc
     1081 cgaggcagtt gctcaagcag tgctttcagt ctcagacaac aaatatatta tactgctctt
     1141 gatggctgtg atagtattga ttgccggtgt tttcatggaa acatcctcgg ctatcatact
     1201 tattactccg gtatttcttc cacttgtaag aatgctaaat atcgacctta ttcactttgg
     1261 tttgatattc acaataggca ttgcaatcgg aatgattacg ccgcctgttg ccatcgacct
     1321 ttttgtggca tcttcaataa caggaatgcc aattgaaaga atagctaaaa aggttgttcc
     1381 atatcttata ggcttgataa tagtattttt catttacatt tatctgccgc ttttcatacc
     1441 gtcattaata ctataannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnggat
     1561 ttataaaatt atttgcaact gttaacaaaa aagatataat atcataactt aattaaaaaa
     1621 ggagtttttc atgcgaatta taattgatgg agatgcatgc ccgcagaagg taagagaaat
     1681 atgtgaaaaa gcagccaagg attttggagt gggcttgata attgtttcag acattgacca
     1741 ttatattgtc agtgattttg aagttattgt tgttgagcag ggaagagatt ccgttgatta
     1801 taaaatagtt caaatatttc agcaaggaga tatattaata actcaagact acggacttgc
     1861 aagccttctt ttggggaaag catcagcaat cattcataca gcagggtttt ttataaataa
     1921 gaataatatt gacagcctgc ttcaatccag atatataagt gataaaataa gaaaacaagg
     1981 cggaaaatca aagggaccgt caaaaagaag aaaagaacag gatgaaaact ttaaaaaatg
     2041 cctgtataaa gttttaagag aatatatgtc aaataaaaag ggcaggtaat tctaaacgaa
     2101 aaaaatcata tcataggaag ggggacataa agtaatgtcc cttactttat tcctctaagc
     2161 tccctaacac atcatcaaca attctgtctg atgaagattt attgtacatt ttcttcatgt
     2221 ttttgcgcat tgcggacaga agttcaggat tatttattaa atcaatagcg ctgtttataa
     2281 tttgataagt attgttggct attattgctg cacctttttt ttcaaaatat cttgcatttt
     2341 ctttttcctg accgggtaca ggcttgatta taataagagg cagctgtacc gataatgctt
     2401 ccgtcaatgt aattccaccc ggtttggtaa tcatacagca tgcaattttg tatagttcat
     2461 gaatatcttc aacaaacccg aacactttta gatttctgag cctcaaggat tccaattttg
     2521 ttttctgata tatattgttg ccgcaaacta ctgctacctg aatattttta tttttgcaca
     2581 actccgtaca tactttttta atatccttta atacaccaaa tgcgcctgag tttataagaa
     2641 ttattctttt tcgtctgtta aatttatatt tggcatggag tttcgaaata ttgtcaattt
     2701 cttcaaaagc atccctgatg ggtattccgg atacgactat tttttccgga ggtatattca
     2761 tttttttgaa ttcttcctgc aattcgtatg tcgcaatata aaacttgtca atttccttgc
     2821 ttatccaaag tttatgagca taatagtcgg ttactatatt gaaaacaggt atattttctc
     2881 cttgttcatt cttcagcttt aaagatgcca aaatggggaa agtattaatt ataacatcag
     2941 gtttaaatac ttcagatata tcctttaatt ttgaaaatcc gaaatttctg tataattcaa
     3001 ctaccttctt atccgttagt ttttctgagc cataataaag ttttgagtaa gcttttccaa
     3061 aatcatagct tctgatatat gcctttttta tagcttcggt gattttggga tgtgtttcag
     3121 taaacaaatc tacaacaacc acattgttat accccttttt aataaatgca ttttttattg
     3181 cagttgcaac cattttatgg ccgtttccaa agggggcggt ggttatcaat atatttttat
     3241 ccataatacc tcccgaaatg attaatgttg ttctgtttgt acgtcgcttt taatattata
     3301 atatataact cggataaaag aaagagagta tattgtcagg gagggaaaat gtataaagaa
     3361 tttctggaga ccttcatctt ggtctttgtt gctgaaatgg gagataaaac ccagctgatg
     3421 cttatgacct tggcagccaa gtataaaata attgatgtgc ttttaggaat ttttctcggt
     3481 gttttattaa atcatggaac tgcggtcttt ataggcagct gtatctctaa tttaatagat
     3541 gaatatcttc ttcatgtatt tgcaggtgtt gttttcataa tttttggaat tattactatt
     3601 gtatttgaag acgaggacga aaacaaaaag caatacggcc tgggtcctgt tattaccact
     3661 gctcttactt tttttctcgg agaaatggga gacaaaactc agcttacatg catgacctta
     3721 tccatggatg catactaccc ttcagcagta ttggcaggct ccgtaacagc aatgcttgca
     3781 ataggtttgg caggaattat tatcggaact tcattgacta aatttattcc ttcctatata
     3841 ataaaaacaa tatccggact gatatttata atattcggag tagtaaggat gataatataa
     3901 atgaatttaa aaaacataaa agataagctt aaaaatataa aaccgaaacc catagatttt
     3961 gatgtaagct ttgcagtatt ggttcccata attgaaattg acggagaatt aaacttaata
     4021 ttcgaggtta gatcaaattc cattactcaa ccgggagaaa tttcgtttcc gggaggaaga
     4081 attgaagaag gtgaaagccc aaggaaagct gcaataagag aaacttcaga agagctattg
     4141 ttaaataagg aaaatattga aataatttca gagctgaatt atgcttcaag caaaagcggg
     4201 gcttttgtat ttacattttt agggctgatt aaggatgtcg acccggatga aatagaatac
     4261 agcattgatg aagtttcgga agttttcttt gtacccttgt catttttttt agaaaatcgg
     4321 cctgaaaaat attatatgaa ttattatcca aaggccgaca aggattttcc ttatcatatg
     4381 gttaacaacg gggaagatta caattggggg aatattcgat atcctgttta tttttacaag
     4441 tactacaatt atataatatg ggggttgaca gctaaaataa cttacagcct tattaaagag
     4501 cttacaaaca gtgacgtcaa aaagaggaat aataatgaaa gctagtatat tcaacattca
     4561 gaaattttct atacatgacg gacccggaat aagaacgacg gtattcttta aaggatgccc
     4621 cttacagtgc atatggtgcc ataatccgga aagccaaaat ttaggtaaag aaattttata
     4681 cgacaaaaat aaatgcacat tatgcggatc ttgtattaag atatgtcaaa acaatgctat
     4741 agaattaaaa gacaatgacc ttgaaataaa tatggacaag tgtacttttt gcggggattg
     4801 cactgtttgc tgcataaact ccgcaaaaca gattgccgga aaagaatata ccgttgatga
     4861 agtaatgaaa gaagtactaa aagacagagt attttataaa aactcaaagg gcggtgtaac
     4921 attgtccgga ggagagcctc ttatatatgc agcctttgtc gaggaattgt taatggagtt
     4981 aaaaaaagaa aatattcata cagcagttga tacttgcgga gcggttgact ttaaagtgtt
     5041 ggaaaggata tctaaatata cggacttatt tctatatgac ataaaatcca tggatgaaga
     5101 gaagcatata ctatatacgg g
//
LOCUS       DAOSOW010000088         5118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_16442_length_5118_cov_3.259530, whole genome shotgun sequence.
ACCESSION   DAOSOW010000088 DAOSOW010000000
VERSION     DAOSOW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5118
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16442_length_5118_cov_3.259530"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="PLD67_03370"
     CDS             complement(<1..1244)
                     /locus_tag="PLD67_03370"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOT21594.1"
                     /translation="MQNSLEQITIKLGGLTCTACEYKIEKALKKLDGVDSVKVSFASS
                     MAEIIYNSDKRDLNTIYDTIEKTGYEVVYDYDEIEEDSPNTSTFLILLIIAGLYIIIK
                     NTVGFNFLPQITQSMGYGMLFIAGLFTSIHCITMCGGISLSQCAGCGDCENNVSSKLK
                     PGFLYNLGRVISYTILGGIVGALGSVFSLSLKGKSIISLIAGAFMIVMGINMLNITPA
                     LRKLMPRLPKSITDKIDSKKNGNGAFIVGLLNGFMPCGPLQAMQIYALGTGSFLTGAL
                     SMFIFSLGTVPLMFAFGAFSSLISLKFTKQMMKITSVLVIILGVVMGNRGLLLSGINV
                     SEAILSPMGISLSKDNKNADAVIKDGYQTVTTNLEPGRYQPINVYAGVPLKWTIVVEE
                     GKLNGCNNELIIPAYGKQQKLE"
     gene            complement(1253..1780)
                     /locus_tag="PLD67_03375"
     CDS             complement(1253..1780)
                     /locus_tag="PLD67_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2318 domain-containing protein"
                     /protein_id="HOT21595.1"
                     /translation="MKANKTKKVKKNNNLVLGVVAVIIIGIAAFTFLNSGKDDEIAKN
                     SKPTAISATDENGDMVIQTSDITEIASFYGYDELDTYIEVLAVKASDGTIRTAFNTCQ
                     VCYSSGRGYYVQQGDYLICQNCGNRFKLDDVEVTRGGCNPVPILDDMKEVTDTTITIP
                     KDLFAEAEVIFANWR"
     gene            1961..3982
                     /gene="fusA"
                     /locus_tag="PLD67_03380"
     CDS             1961..3982
                     /gene="fusA"
                     /locus_tag="PLD67_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT21596.1"
                     /translation="MKTYNADKIRNVALLGHSSCGKTTITEALLLNTKVTNRMGKVED
                     GNTVSDFDKEEKERQVSIGTSVIPIEYKDTKINFLDTPGYFDFVGEVYGALRVADSAV
                     LVIDASSGIEVGTEKAWKYLEQRNMPRIIFINKLDKENIKFDELLDNLRDTFGKKVIP
                     FTLPIGKGENIDGYMDILKGQSYIDSKPVETFPDTAAKAEELKAILMESVAETDEELL
                     EKYFEGEEFTEEEIQTGLKKAVLEGELVPVIVGSAIKGLGIDMLMNTVVEYLPNALDA
                     KSLKGSPSDSLSAFIFKTIVDPFVGKISLYKVMSGKMKKDTDVYNANTGDTERIGSVF
                     SLRGKEQLEVSEVEAGDIGATSKLQYFKTGDTISLKTAPVSYDKIDFPKPCYFMAVKG
                     KTKDSDEKIGTGLQRLNEEDPTFSIERNIETKELIVGGQGNIQLDVLASKLKNNYKVD
                     VELSPPKVAYRETIKGKSDVQGKHKKQSGGAGQYGDVRIRFEPSQEEFEFTEEIFGGS
                     VPKNYIPAVEKGLRESLEKGVLAGCKVVNVKATLYDGSYHEVDSNEMAFKIAASLAFK
                     KGMLEAKPILLEPIAKVEINIPDEYLGDIMGDMNKRRGRILGMEQQADGSQLVKAEAP
                     MAEMYTYAIELKSMTQARGTFEVEFLRYEEMPNNLAEKVIAEYKAANEN"
     gene            complement(4148..4762)
                     /locus_tag="PLD67_03385"
     CDS             complement(4148..4762)
                     /locus_tag="PLD67_03385"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT21597.1"
                     /translation="MNNPWEDINLEDYEKHMSLDSVFQLQAMNQMMKEQFYTYPIKSI
                     MIFGIAGGNGLEHINNQVINKVYGIDINEKYLNTCKKRYPELEGILETLCKDLTKDIE
                     LPYADLLVANLLIEYIGYEYFQKAVKKVNPKYVSCIIQVNTNDSFVSDSPYLHVFDRL
                     DEILHPVEESGLVEAMRNINYKKDRLQERKLPNGKKFIRIDFTR"
     gene            complement(4849..>5118)
                     /locus_tag="PLD67_03390"
     CDS             complement(4849..>5118)
                     /locus_tag="PLD67_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HOT21598.1"
                     /translation="SEISSILDLLINDLNKRLSDKQLTVSVTERAKNYIVDAGYDPVY
                     GARPLKRFVQSSVETLIARLMISEDIEPNTTLEVDYDGRQLIINH"
BASE COUNT         1745 a          883 c          887 g         1603 t
ORIGIN      
        1 atttcaagtt tttgctgctt cccatatgcc ggaataatta attcgttgtt acagccattt
       61 agttttcctt cttcaacaac tatagtccat ttcaaaggaa cacctgcata gacgttaata
      121 ggttgatatc ttccgggttc aagatttgtt gttacagtct gataaccatc ttttataaca
      181 gcatcagcgt tcttattgtc tttagataaa cttatcccca taggtgacaa tattgcttca
      241 gatacattaa tacctgacag caataagcct ctgtttccca taactacacc caatattata
      301 actaaaaccg atgtgatttt catcatctgt ttagtaaatt tcaagcttat taatgagctg
      361 aatgcaccaa atgcaaacat taatgggaca gtacccagac taaaaataaa catggataaa
      421 gctcccgtta aaaagcttcc tgttccaaga gcatatattt gcatggcctg caaaggaccg
      481 caaggcataa atccattaag taatcctact ataaatgctc cattaccgtt tttcttgctg
      541 tcaattttat cggttatact cttaggcagg cggggcatga gttttcttaa cgcaggcgta
      601 atatttagca tattaatacc catgacgatc atgaaagcac ctgcaataag cgaaattatt
      661 gatttacctt taagtgataa actaaaaaca gaaccaaggg caccgactat tcctccaaga
      721 attgtatagg atataaccct tccaagatta tataaaaaac ccggctttag tttacttgat
      781 acattgtttt cgcaatcacc gcatccggca cattgtgaca aactaatacc cccgcacatt
      841 gtaatacagt gtattgacgt aaacagtccg gctataaaaa gcattccgta acccatgctt
      901 tgagtaattt gcggtaaaaa gttaaatccg acagtatttt taataattat atacaatccg
      961 gcaattatta ataaaattaa aaaggttgat gtattaggtg aatcttcttc aatttcatca
     1021 taatcatata caacttcata tcctgttttt tcaatggtgt cataaatagt atttaaatct
     1081 cttttatcgc tattgtatat aatttctgcc atggatgaag caaagcttac tttaacggaa
     1141 tcaacaccat ctaatttttt taatgccttt tctattttat actcacaagc cgtgcaggtt
     1201 aatccgccta attttattgt tatttgctct aatgagtttt gcataatata ccttatctcc
     1261 aatttgcaaa aataacttca gcttcggcaa ataagtcttt aggaattgtt attgttgtat
     1321 cggttacttc tttcatatca tcaagtatgg gaaccgggtt acatccaccc cttgtcactt
     1381 caacatcatc caatttaaac ctgttgccgc agttttgaca aatcaggtaa tctccttgct
     1441 gaacataata tcctctgcct gatgaatagc aaacctggca agtattaaat gcagttctta
     1501 ttgtcccgtc tgatgctttt accgccaata cttcaatata agtgtcaagc tcatcataac
     1561 catagaatga tgcgatttca gttatatctg aagtttgaat aaccatatct ccattttcat
     1621 cggttgctga tatggcggtt ggttttgagt ttttcgctat ttcatcatct tttccggaat
     1681 ttaaaaaggt gaatgctgct ataccaataa ttataacagc cacaacacct aatactaaat
     1741 tattgttttt cttaactttt tttgttttat tagctttcat atcgggttcc tttccatatt
     1801 aatttgaaaa attataaaaa aactttatga agattttgtg aagatatgtt actctattct
     1861 ttatgcatct aatatcaaca atgtaaaatt gtattgatta tttaataaat ttaatgtaaa
     1921 atgaaataaa taagcacaac ccataggagg aataaagtta atgaagacat ataacgcaga
     1981 caaaatcaga aacgtagccc tgcttggcca cagtagctgt ggcaaaacta caattacaga
     2041 agctcttttg ttaaatacaa aagtaacaaa cagaatgggt aaagtagaag acggaaatac
     2101 tgtatccgat tttgataaag aagaaaagga acgtcaagtt tcaattggaa catcagtaat
     2161 accgattgaa tacaaagata ctaaaattaa tttcttagat actcccggat attttgactt
     2221 cgttggtgaa gtttatggag cacttagggt tgcagacagt gcagtattag ttatcgatgc
     2281 cagctccgga atagaagtag gaacagaaaa agcatggaaa tacttagagc aaagaaatat
     2341 gcctcgaata atattcatta ataaattaga caaagaaaat atcaagtttg atgaactgct
     2401 tgataattta agagatacat ttggcaagaa agtaattccg tttactctac ccataggcaa
     2461 gggagaaaat attgacggat atatggatat attgaaagga caaagctata tagattcaaa
     2521 accggttgaa acattcccgg acacagcggc aaaggcggaa gaattaaaag caattctaat
     2581 ggaatccgta gccgaaacag atgaagaatt acttgaaaaa tactttgaag gcgaagaatt
     2641 tactgaagaa gaaattcaaa ccggattaaa aaaagcagta ttagaaggcg aattggttcc
     2701 tgtaatagtt ggttccgcaa taaaaggatt aggcattgac atgctgatga atacagtagt
     2761 agagtatttg cctaacgcct tggatgcaaa atcacttaaa ggaagtcctt cagattcatt
     2821 atctgcattt atattcaaga ctatagttga cccctttgta ggaaaaattt ccttatataa
     2881 ggtaatgtca ggaaagatga aaaaagatac ggatgtttat aatgcaaaca ccggtgatac
     2941 tgagagaata ggcagcgtat tttccttaag aggaaaagag caattagaag tttctgaagt
     3001 tgaagcaggt gatataggtg caacttccaa gcttcagtat tttaaaaccg gtgatactat
     3061 ttcattaaag actgctccgg tatcttatga taaaattgat tttccgaaac cttgctactt
     3121 tatggcagtt aaagggaaga caaaagacag tgacgaaaaa atcggcacgg gacttcaaag
     3181 acttaacgaa gaagacccga cgttctcaat agaaagaaat attgaaacta aagagcttat
     3241 agtaggcgga cagggaaaca ttcagttgga tgtacttgcc agcaagctta agaataacta
     3301 caaggttgat gttgaactca gtccaccgaa ggttgcatac agagaaacaa ttaaaggcaa
     3361 atcagatgtt cagggaaaac acaaaaaaca atcaggcggc gcaggccagt atggagatgt
     3421 tcgtatcaga tttgagccat ctcaggaaga atttgaattt actgaagaaa ttttcggagg
     3481 atcagtaccg aaaaactata tcccggcagt agaaaaggga cttagagaat cacttgaaaa
     3541 aggtgtattg gcaggatgta aagttgttaa tgttaaagca acattgtatg acggctctta
     3601 ccacgaagtt gactcaaacg aaatggcttt taaaattgca gcatcattag cattcaaaaa
     3661 aggtatgctg gaagcaaaac ctatattgct tgagcccatt gcaaaggttg aaatcaatat
     3721 tcccgatgag tacttgggag atataatggg tgatatgaac aagagaagag gaagaattct
     3781 cggtatggaa cagcaagcgg acggaagcca gctggtaaag gcagaagctc caatggcaga
     3841 aatgtacaca tatgcaatcg agcttaagtc catgactcaa gcaagaggta cttttgaagt
     3901 cgaattctta agatatgaag aaatgccgaa caacttagct gaaaaagtta tagctgaata
     3961 caaggcagcg aatgaaaact aaagacatgt acgactataa taaagttatt aaataataaa
     4021 aagttgttgt aaaaaatcac ttaaaagatt tattattatc ttttaagtga tttgtttttc
     4081 ttactaatta gcttaatttg aagattttat aataaattat gttcataata tttgcaataa
     4141 gccaaattta acgagtaaaa tctattctta taaatttttt cccgtttggt aattttcttt
     4201 cttgaagcct atcttttttg tagttgatat tacgcatagc ttcaaccaac ccactttctt
     4261 ccacagggtg gaggatttca tctaaacggt caaatacatg gagatatgga gaatcggata
     4321 caaaagaatc atttgtattg acttgaataa tacacgacac atattttggg tttactttct
     4381 taaccgcttt ttggaagtat tcatatccaa tatactcaat taagagattt gccacaagta
     4441 aatctgcata tggcaattct atgtctttag ttaaatcttt acaaagtgtt tcaagtatgc
     4501 cttccagttc cgggtatcgt tttttgcacg tattcaaata tttttcatta atgtcgattc
     4561 cataaacttt attgataacc tggttattaa tatgttctaa cccgtttccg cccgctattc
     4621 caaaaatcat aatggatttt atcggatatg tataaaattg ttctttcatc atttgattca
     4681 ttgcttgaag ctgaaacaca ctgtctaaac tcatatgttt ttcataatct tccaagttaa
     4741 tatcttccca aggattattc atttaaagta cctcacaaat acctatttat tggtttttaa
     4801 aaacaagtat gtaaacctat ctgctgaatc aagtcagtta tttataactc aatgattaat
     4861 aatcagctgc cttccgtcat aatccacttc aagggtggtg tttggttcta tatcttctga
     4921 aatcatcaat cttgctatca atgtttccac gctgctttga acgaaacgtt tcaatggtct
     4981 tgcaccgtaa acagggtcat atcccgcatc aacgatataa ttttttgctc tttcggttac
     5041 tgaaacggta agctgcttat cgctgaggcg cttgttaagg tcattaatca ataaatccag
     5101 tatagatgat atctccga
//
LOCUS       DAOSOW010000089         5068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_16655_length_5068_cov_4.399561, whole genome shotgun sequence.
ACCESSION   DAOSOW010000089 DAOSOW010000000
VERSION     DAOSOW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16655_length_5068_cov_4.399561"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1959
                     /locus_tag="PLD67_03395"
     CDS             <1..1959
                     /locus_tag="PLD67_03395"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOT21599.1"
                     /translation="VVEDTGITFDEIKREFGEEIANMVDGVTKLSKVKYRNKEERQAE
                     SLRKMIVAMSKDIRVVIIKLADRLHNIRTLQYMPKEKQYQKAMETIEIYAPIANRLGI
                     ASIKWELEDLSLKYIDPEGYEDLLKKVQEKSEKRKDYISHIIDIINKKLQESGMEADI
                     KGRPKSLYSIYKKMYYKNRLFEQIYDLIAIRIIVENIKDCYGALGIVHTLWKPVPGRF
                     KDYIAMPKPNMYQSLHTTVIGPEGEPFEIQIRTLEMHRTAEYGIAAHWKYKEGIDDKT
                     KYEEKLNWLRQMLEWQQETNDPQEFMEALKIDLYTDEVFVFTPKGEVINLPTGSTPID
                     FAYRVHTAVGNKCVGAKVNGRIVPLDAKLKTGDQVEILTSQSSVGPSRDWLKIAASSQ
                     AKSKIRKFFKDRDKDFNVEKGKELVEREIKKQGYQVADLLKDDWIKTVADKYNMSAAS
                     NLFAAVGSGGITESQIVNRLKLIYLEKNKDKLELDRIENLEKSLAKSPQRERHTSGVI
                     IKGIDNIKVRFSKCCNPVPGDAIVGYITRGRGVSIHRADCTNIHDLSDSEDQRFIEVQ
                     WDNKEKATFISELQIKALDRPKLLQDITSLYVDAKLNAVSLNLRINKEKVAIVDIGFE
                     INDIKQIEDLIKKMKKLDGVLEVYRNKK"
     gene            1982..2431
                     /gene="dtd"
                     /locus_tag="PLD67_03400"
     CDS             1982..2431
                     /gene="dtd"
                     /locus_tag="PLD67_03400"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOT21600.1"
                     /translation="MRAVVQRVKSAHVKVKDKIIGSIGQGILLFLGVEETDQETDLEY
                     MCEKIPNLRIFEDENGKMNKSLLDVEGSILVISQFTLLGDARKGRRPSFTDAAKPEKA
                     IPMYEKFIACMKEKNITTQSGEFGAEMEVELINDGPVTILLDSKKVF"
     gene            2441..3061
                     /locus_tag="PLD67_03405"
     CDS             2441..3061
                     /locus_tag="PLD67_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT21601.1"
                     /translation="MIFEALEVGIYCANCYIVGSEETKEAAIIDPGADFNRIEGKLHE
                     LGLTPKIIILTHHHGDHIGAVKEFTDKYGLKVYIHKEDAEALNNSSMNFTKSMFGKNI
                     LIKADVELEDGDVIYLGELKFEIIHTPGHTKGGICIKVGNIMMTGDTLFNSSIGRTDL
                     PGGSYEEIISSIKKKIFKFDDDTVIYPGHMSPSTIKKEKKFNPFVK"
     gene            3082..4548
                     /gene="hemZ"
                     /locus_tag="PLD67_03410"
     CDS             3082..4548
                     /gene="hemZ"
                     /locus_tag="PLD67_03410"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HOT21602.1"
                     /translation="MFKFYFFGHDYEYEARNALRVFDLNIDYEIMHGSFDGRSLSVLS
                     YLEEGEIISAKAQLYNDCELLYESCISENDIVLEKYSLKKLKKTLVIKTIHNALKSYY
                     KVYPDYGILTGVRVVKILITAKKYSKSNEEINKILRDTYEVKEEKIKLLWDILNIEEK
                     YIDENINKKNYNLYIGIPFCPSKCSYCSFTSYVNCTDKKIFTYLDTLIYEIEKTIELA
                     KKKELNLHTIYIGGGTPSVLNEEQIETIFNAIKKYYDLSQVKEITFEAGRPDTINSEK
                     LICLKNNFVNRISINPQTMNDNTLLSIGRKHLGFEVVEKYLLAREIGFQTINMDIILG
                     LPGEDESDVKNTIEIIAGLNPENITVHSLAYKKHSELTRESAKLFKDYELIKKMHHVI
                     KEVCEVKCYKPYYMYRQKNIKGNSENIGYTKKDRECIYNIVMIEELETILGCGLGASS
                     KIMTGENKHETVRNFKSLEEYSKRIDEIINKKQTLLGG"
     gene            4549..4809
                     /locus_tag="PLD67_03415"
     CDS             4549..4809
                     /locus_tag="PLD67_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT21603.1"
                     /translation="MKKIKLFYIEGCPHCRKAFRILDELKAKNPEYSKIDVEYIDENK
                     DVVSANLYDYYYVPTFFVDDVKMHEGVPTEEKIERVLKEAIK"
BASE COUNT         1998 a          688 c         1019 g         1363 t
ORIGIN      
        1 gtagtggaag atacgggaat aactttcgat gaaattaaaa gggaattcgg cgaggaaatt
       61 gccaacatgg tggatggcgt cacaaaactg agcaaggtta agtacaggaa caaagaagag
      121 cgccaagcag aatctttaag aaaaatgata gtggcaatgt caaaggacat aagggttgtt
      181 ataatcaaat tagccgaccg acttcacaat atcaggacat tacaatatat gcccaaggaa
      241 aaacaatacc aaaaggcaat ggaaactatt gaaatatatg cacccatagc aaaccgcctg
      301 ggtattgcgt ccattaaatg ggaattggaa gatttgagct taaagtatat agatcctgaa
      361 ggatatgaag atttgcttaa aaaagttcag gaaaaaagcg aaaaacgaaa agattatatt
      421 tcgcacatta tcgatattat aaataaaaag cttcaggaat cgggaatgga agccgacatt
      481 aagggcagac ctaaaagttt atacagtatt tacaaaaaaa tgtattataa aaacagattg
      541 tttgaacaaa tctatgattt aattgccata agaattattg tggaaaatat aaaggattgt
      601 tacggagcat tgggtattgt tcatactctt tggaaaccgg tgccgggaag atttaaagat
      661 tatatagcca tgcctaaacc aaatatgtat caatctttgc atacaacagt aataggtcct
      721 gaaggcgagc ctttcgaaat tcaaatcagg accttggaaa tgcatagaac tgctgaatat
      781 ggtattgcag cccactggaa atacaaagaa ggtatagatg ataaaaccaa gtatgaagaa
      841 aagttgaatt ggttgagaca aatgcttgaa tggcagcagg agactaacga ccctcaggaa
      901 tttatggaag ctttgaaaat tgacttatat actgacgaag tatttgtatt tactccaaag
      961 ggagaagtga taaatttgcc tacaggatcc acacccatag attttgctta cagagttcat
     1021 actgcagtag gaaacaagtg tgtgggagca aaagttaacg ggcgaattgt tccgctcgat
     1081 gcgaagctta aaacaggtga ccaagtagaa atattaacat cccagtcaag tgtgggacca
     1141 agcagggatt ggctgaaaat tgccgcgtca agtcaggcaa aatctaaaat cagaaaattt
     1201 ttcaaagata gagataaaga ttttaatgtt gaaaaaggaa aagaattagt agaaagagaa
     1261 attaaaaagc agggatacca agtagcagat ttgcttaagg atgactggat taaaactgtt
     1321 gccgacaaat acaatatgtc tgctgcaagc aacctctttg cggcagtagg cagcggaggt
     1381 ataacagaaa gccagatagt aaacaggctt aaattaatat atcttgaaaa gaacaaagat
     1441 aaactcgaac ttgatagaat tgaaaattta gaaaagagtt tggcaaaatc accccagagg
     1501 gaaagacata catccggcgt tataataaaa ggtattgata atataaaagt tcgtttttcc
     1561 aaatgctgca atcccgttcc cggagatgcc atagtgggat atatcacaag aggcagagga
     1621 gtgtccattc acagggctga ctgtacaaat attcatgatt taagcgattc tgaagaccag
     1681 agatttattg aggttcagtg ggacaataaa gaaaaggcaa cattcatcag cgaacttcag
     1741 attaaagcat tggacagacc gaaacttttg caggatataa cttctttgta tgtagatgca
     1801 aagcttaatg ccgtatcgct taatttaaga ataaataagg aaaaggtagc aatcgttgat
     1861 atcggatttg aaataaatga tattaaacaa atagaagatt tgattaaaaa gatgaaaaag
     1921 ttagacggag tattggaagt ctacagaaat aaaaagtgac gaaaaaatga aaggatgaaa
     1981 aatgagagca gttgtgcagc gcgtaaaaag cgcccacgtt aaagtaaagg ataaaataat
     2041 aggaagtata ggacagggaa ttttgctatt tctcggtgtt gaagaaacag accaagaaac
     2101 ggatttagaa tatatgtgtg aaaagattcc aaatcttaga atatttgaag atgaaaacgg
     2161 aaaaatgaat aaaagcttat tggatgttga aggaagtata ttggttattt cacagtttac
     2221 attgctgggt gatgcaagaa aaggaagaag gccaagtttt acggatgcag ccaaaccgga
     2281 aaaggcaatt cctatgtatg aaaaatttat tgcctgtatg aaggagaaaa atattacaac
     2341 acaatcagga gagtttggtg ctgaaatgga agtagaatta attaatgacg gtcctgttac
     2401 catacttctt gacagcaaaa aagtttttta ggaggttctt atgatattcg aagcattgga
     2461 agtcggtatt tattgtgcaa actgttatat agttggaagt gaagaaacta aggaagcagc
     2521 cataatagac cccggtgctg actttaatag aattgaaggg aaactgcatg aattgggatt
     2581 aacccctaaa ataataattt tgactcatca tcatggtgat catattggag cggtgaaaga
     2641 atttaccgat aaatacggat tgaaagttta tattcataag gaagatgcgg aagcactcaa
     2701 taacagcagc atgaatttca ctaaatcaat gttcgggaag aatattttaa taaaggcaga
     2761 tgttgagtta gaggatgggg acgtaatata cttaggtgaa ttaaaatttg aaataattca
     2821 tactcccgga catacaaagg gaggtatctg catcaaggtt ggaaatataa tgatgacggg
     2881 agatacttta ttcaactctt ccattggaag aaccgacctt cccggaggct cctatgaaga
     2941 aattataagc tcaattaaaa agaaaatatt taaattcgat gatgatacgg taatttatcc
     3001 gggtcacatg tcaccttcaa caataaaaaa agaaaagaaa tttaatccgt ttgtaaaata
     3061 agatgctatc aggtagaaaa aatgtttaag ttttattttt ttggtcatga ttacgaatat
     3121 gaagctagaa atgcgctcag agtattcgat ttaaacatag attatgaaat aatgcatggc
     3181 tcctttgacg gaaggagctt atctgtttta tcatatcttg aagaaggcga aattataagt
     3241 gcaaaggcac agctttacaa tgactgtgaa ttgttgtatg aatcatgtat cagtgaaaat
     3301 gacatcgttt tggagaaata cagcttaaaa aagctaaaaa aaacattggt aattaaaaca
     3361 atacacaatg ctttgaaatc atattacaag gtctatcccg attatggaat attaaccgga
     3421 gtcagagtag ttaaaatttt aatcacagca aaaaaatact ctaaaagcaa cgaagaaata
     3481 aataagatac tgagagatac ctatgaagtg aaagaggaaa aaataaagct tctatgggat
     3541 atattaaata tagaagaaaa atatattgat gaaaatataa ataaaaaaaa ttacaactta
     3601 tacataggta ttcctttttg cccttctaaa tgcagctatt gctcctttac ctcatatgta
     3661 aattgtactg ataaaaaaat atttacctac ttggatactt tgatatatga aattgaaaaa
     3721 acaattgaac ttgcaaaaaa gaaagaatta aacttacata cgatttatat aggcggcggg
     3781 actccttcag tattaaatga agagcagata gaaacaattt ttaatgcgat aaaaaaatat
     3841 tacgatttgt ctcaagtaaa ggaaattact tttgaagcag gtagaccgga taccataaat
     3901 agtgaaaaac ttatttgtct taaaaataat tttgtaaaca gaatatccat aaatccccaa
     3961 acgatgaatg ataatacatt attatcaatc ggaagaaaac acttgggctt tgaagttgtt
     4021 gaaaagtact tattagctag agaaatcgga tttcaaacaa taaatatgga tattattttg
     4081 ggactgccgg gagaagatga atcagatgta aaaaatacaa ttgaaataat tgcggggtta
     4141 aatccggaaa atattacagt ccactcattg gcatataaaa aacactctga gcttaccaga
     4201 gaaagtgcaa aactttttaa ggattatgaa cttataaaaa aaatgcacca tgttataaag
     4261 gaggtttgcg aagttaaatg ttacaaacca tattacatgt atagacaaaa gaacatcaag
     4321 ggcaactccg aaaacatcgg ctatacaaag aaagacaggg agtgtattta caacattgta
     4381 atgatagaag aattagaaac aatacttggg tgcggtcttg gtgcttcaag taaaataatg
     4441 acaggtgaaa acaagcatga aactgtacgc aattttaaaa gcctggaaga atattcaaag
     4501 agaattgatg aaataataaa taaaaagcaa actctattag gaggataaat gaaaaaaata
     4561 aaactttttt atatagaagg ctgtcctcat tgcaggaaag catttagaat acttgatgaa
     4621 ttgaaagcta aaaatcccga atactcaaaa attgatgtgg aatacataga tgaaaacaag
     4681 gatgtagtat cagcaaattt atatgattat tactatgttc caacattttt tgtggacgat
     4741 gtgaagatgc atgaaggggt accgacggaa gaaaaaattg aaagagtatt gaaagaggca
     4801 ataaaataaa tcaaaaaatc aggacagctc gtcctgattt tttttcaata aaattatttt
     4861 tcacagtgct ttgcatctgc ttctgtttca aaataaaatg taccctatag aatagacagc
     4921 agcagttttc aaataccctt agtttcggac aagtgtgttt taaaaatcct tagttttgga
     4981 caatcaagtt tttgaaaccc ttcgtttcgg acataagagt tttttgactc caattcagat
     5041 aggtaataga ataaacatta gatgaatt
//
LOCUS       DAOSOW010000090         5056 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_16699_length_5056_cov_3.846231, whole genome shotgun sequence.
ACCESSION   DAOSOW010000090 DAOSOW010000000
VERSION     DAOSOW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5056
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16699_length_5056_cov_3.846231"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLD67_03420"
     CDS             complement(<1..180)
                     /locus_tag="PLD67_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOT21604.1"
                     /translation="MKQIIKEAAYKLGIETVGITGISDYSYLEELLTNRKINNQCCEF
                     EEQDIKKRINAKNLFP"
     gene            complement(296..1318)
                     /locus_tag="PLD67_03425"
     CDS             complement(296..1318)
                     /locus_tag="PLD67_03425"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005541591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOT21605.1"
                     /translation="MEKKDIKWSELGFGYIKTDKRYYSYWKDGKWDDGQLSEDNKVHI
                     SEGSTCLHYGQECFEGLKAYSTKDGGIQLFRPDENAKRMQKSCERLLMPIVPVEKFID
                     ACVQVVKANAAWVPPYGSGATLYLRPYVIGIGDNVGVKPAEEYLFGIFCTPVGPYFKG
                     GMNPVNFITTDQDRAAPNGTGKIKVGGNYAASLYSHFEAVKKGFADCIYLDPSTHTKI
                     DEVGAANFFGITKDNKFVTPRSSSILPSITRISLMQVAKDYLNMEALETDIYVDELDN
                     LKEAGACGTAAVITPIGGIMHKDKFHVFYSETEVGPVTKKLYDTLYGIQMGDIEAPEG
                     WIYKVM"
     gene            complement(1525..2067)
                     /locus_tag="PLD67_03430"
     CDS             complement(1525..2067)
                     /locus_tag="PLD67_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOT21606.1"
                     /translation="MEQKVIMAGFGGQGIMAIGRLLAYAGMLEDKNVTWMPSYGPEMR
                     GGTANCAVVVSEEEIGSPLISKDGTAAIVMNLPSMIKFEKELVPGGKLIVNKSLIDVE
                     PTRNDLDVYYIPANELALELGNGKVANMVMLGAYLEITKAVEIESVLKAFVKVFGENK
                     ADLIPLNKEALLKGAQAAKK"
     gene            complement(2069..2815)
                     /locus_tag="PLD67_03435"
     CDS             complement(2069..2815)
                     /locus_tag="PLD67_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOT21607.1"
                     /translation="MTIVYEKSKGLTDTPFHYCPGCTHGVIHKLVAESLVELGVLDDA
                     IGVAPVGCSVLAYNYFNCDMQEAAHGRAPAVATGIKRVHPDKVVFSYQGDGDLASIGT
                     AEIVHAAHRGEKITTIFVNNAIYGMTGGQMAPTTLVGQKTTTSPFGRDENHCGKPIRI
                     AEMLATIDGAKFVERVTVDTPANIRKAKKAIKRAFECQIKGEGFAIVEVLSTCPTNWG
                     FTPLKALEWLRDNMIPYYPIGNFRDFGEGK"
     gene            complement(2815..3876)
                     /locus_tag="PLD67_03440"
     CDS             complement(2815..3876)
                     /locus_tag="PLD67_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOT21608.1"
                     /translation="MSKVLMKGNEAVGAAAIKAGCKCFFGYPITPSSEIPEYISRKLP
                     EAGGVFLQAESEIAAINMVYGAGGAGARCMTASSSPGISLKQEGITYAAGAEIPCVIV
                     NMMRGGPGLGSIQPSQADYYQSTRGGGNGDYRTLCYAPSSIQELVDYVMLAFDKADYY
                     RNPVLIAADGMIGQIMEAVEFKEPEPIELPPKDWATTGTGGKRKPNVVNSLYLQAEEL
                     QEHNKRLEKKYKIMEENEVRYEAFGLEDAEVVCVAYGTTSRIVKSAIKQAQAKGIKVG
                     MIRPITIWPYPYDVFKEIGPNAKGILGVEMSTGQLLDDIKIGIAGRLPVYFNGTQGGM
                     VPTPGSILADIEKIAGGTK"
     gene            complement(3889..4104)
                     /locus_tag="PLD67_03445"
     CDS             complement(3889..4104)
                     /locus_tag="PLD67_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013386489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT21609.1"
                     /translation="MAKGKVTFNDDACKGCELCVAVCPVKIIELNKEKINNKGYSPAH
                     VTDQEKCIACGNCAIMCPDSVITVERL"
     gene            complement(4118..4795)
                     /locus_tag="PLD67_03450"
     CDS             complement(4118..4795)
                     /locus_tag="PLD67_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT21610.1"
                     /translation="MVHDKRLVIVIGHYGSGKTEFAVNYAVKMKEIYENVSIADLDIV
                     NPYFRSREKREFFEEIGIKLFDSSIRNTAVDLPALPAELMGVITNPNIKSIIDVGGDP
                     VGARVLARFAEQIKNVDYDMFYVINGNRPDTSTVDGVINYLKMIEATSRLKITGLVNN
                     THMLKATTVEDVEFGHELTKKVSWETDIPIRYEAVIKETADKIKNHEIIEKLFPINLY
                     MREEWMS"
     gene            complement(4825..>5056)
                     /locus_tag="PLD67_03455"
     CDS             complement(4825..>5056)
                     /locus_tag="PLD67_03455"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867445.1"
                     /note="catalyzes the phosphorylation of 2-butanoate to
                     butanoyl phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOT21611.1"
                     /translation="QVAKAIGEYAVVLDGDVDAIIITGGIAYDKDFVKMIEDKVKFIA
                     KVIVYPGEDELLALAEGGLRVLKGVEEAKTYN"
BASE COUNT         1358 a         1149 c          760 g         1789 t
ORIGIN      
        1 gggaaagaga tttttggcat ttattctttt ttttatatcc tgctcttcaa attcacagca
       61 ctgattattg atttttctgt ttgttaaaag ctcttctaaa taggaatagt ctgaaatgcc
      121 ggttatacct actgtttcga ttcctaattt atatgctgct tctttaatta tttgtttcat
      181 aatatcctca tctctattgt ttccatacca tagcatatca taaatatata aaatattgaa
      241 gaaaaaaaaa ttggacatta aatatgtaaa tttaatgtcc atttggagaa aagtattaca
      301 ttactttgta tatccatcct tcaggcgctt ctatatcacc catctgaatt ccatataatg
      361 tgtcatataa ttttttagta acaggtccta cctctgtttc gctgtagaat acgtggaatt
      421 tgtctttatg cataattccg cctatcggtg taataaccgc tgccgtaccg caagcccctg
      481 cttctttcaa attatctaat tcatcaacat atatatctgt ttcaagtgct tccatattta
      541 agtaatcttt agcaacctgc attaaagaaa ttcttgttat actcggaagt attgaagatg
      601 aacgaggtgt tacaaacttg ttgtccttgg taatgccaaa gaagtttgct gctccaactt
      661 catcaatttt tgtatgggtt gagggatcta aataaataca atcggcaaaa ccctttttaa
      721 cagcttcgaa atgtgaatac aagcttgctg catagtttcc gcctacttta atttttcctg
      781 ttccgttcgg tgctgctctg tcttggtcgg tagtaatgaa attaacggga ttcattccgc
      841 ctttaaagta tgggccgaca ggtgtgcaga atattccaaa taagtattct tctgcaggtt
      901 ttacgcctac gttgtctcct atacctatta catacggtct caaatacaat gtagcacctg
      961 acccgtatgg aggaacccat gctgcgttag ctttaacgac ctgcacacag gcatcgataa
     1021 atttttccac gggaacaatg ggcatcagta atctctcaca gcttttttgc atacgctttg
     1081 cattttcatc aggtctgaaa agttgaatac ctccatcctt agtagaataa gctttcaatc
     1141 cttcaaaaca ttcctgtccg taatgcagac atgtggagcc ttcgcttata tgaaccttat
     1201 tatcctctga aagctgtccg tcatcccatt ttccgtcttt ccaataagaa tagtatcttt
     1261 tgtctgtctt aatataacca aatcccaatt ctgaccattt gatgtccttt ttttccatta
     1321 ataagcctcc tttattttta tatttattat ttcgggacat tcctctgccc tcacagactt
     1381 taaatttggg gacatttctc tgacttcata ggtttaccct acagaggaga ggtgtgtccc
     1441 cataccttgt tgccccaata catataatat ttttatatca taacagcttt gattaaaatt
     1501 tccaacactt attttctaaa cgctttattt ttttgctgcc tgagcaccct tcagtaaggc
     1561 ttctttgttc aaaggtatca gatcggcctt attctctccg aaaactttta caaaagcttt
     1621 caatactgat tcaatttcaa ccgctttagt aatctctaaa tatgctccaa gcataaccat
     1681 atttgctact ttaccgttac ctaattccaa tgctaactcg ttagccggaa tataataaac
     1741 atctaaatcg tttcttgtcg gttcaacatc aatcaatgat ttgtttacta ttagttttcc
     1801 gcccggaaca agctcctttt caaatttaat cattgaaggt aagttcataa ctatcgctgc
     1861 agttccatcc ttagaaataa gaggggagcc tatttcttcc tctgatacaa caactgcaca
     1921 atttgctgta cctcctctca tttcgggacc gtaagaaggc atccaagtta cgtttttatc
     1981 ttcaagcatt cctgcatatg ccaataacct tccgatagcc ataattcctt gaccgccaaa
     2041 tccggccata attacttttt gttccatatt atttcccctc cccaaaatct ctgaaatttc
     2101 ctatcggata atatggaatc atattatctc tcagccactc caatgctttt aaaggagtaa
     2161 atccccagtt tgtagggcat gttgacaaca cttctacaat tgcaaagccc tcgcctttga
     2221 tttgacattc aaaagctctc ttaattgctt ttttagcctt cctgatattt gccggggtat
     2281 ctaccgtaac tctttcaaca aattttgctc catcaatagt tgccaacatt tctgctattc
     2341 ttatcggctt tccgcaatga ttttcatctc ttccaaatgg agatgtggta gttttttgtc
     2401 ccaccagtgt ggtaggagcc atctgtccgc ctgtcattcc gtatattgca ttgtttacaa
     2461 atatagttgt aattttctcg cctctgtgtg ctgcatgaac aatttctgca gttccgatag
     2521 aggctaaatc tccatcacct tggtaagaga atacaacctt gtctggatgt actcttttaa
     2581 tacctgtagc aacggcaggt gcccttccgt gagcagcttc ttgcatgtcg cagttaaaat
     2641 aattgtaagc taatacggaa catccaacag gagctactcc gattgcatca tccaaaactc
     2701 ctaattctac caatgattct gcaactaatt tatgaataac accgtgtgta caaccggggc
     2761 aataatgaaa aggtgtatct gttaagccct ttgatttttc atatacaatt gtcattattt
     2821 tgtacctcct gctattttct caatatctgc caagatactg cccggtgttg gaaccatacc
     2881 gccttgagta ccattgaaat atacaggcag tctgccggca atacctattt ttatgtcatc
     2941 cagtaattgt cccgtgctca tttctacgcc aagtattcct tttgcattag gaccaatctc
     3001 tttaaataca tcatacggat agggccatat agtaatcggt cttatcatac caacctttat
     3061 gccctttgcc tgtgcttgct taattgcaga ctttactatt cttgatgtag taccgtatgc
     3121 tacgcataca acctctgcat cttctaagcc aaatgcttca taacgaactt cgttttcttc
     3181 cattatttta tatttttttt ctaatctttt attgtgttct tgaagttctt ctgcctgcaa
     3241 gtaaagtgag tttacaacat ttggttttct ttttccgccg gttcctgttg tagcccaatc
     3301 tttaggaggc aattctatcg gttccggttc cttaaattca accgcttcca ttatttgacc
     3361 aatcattccg tctgcagcta ttaaaacggg atttctgtaa taatcagctt tatcaaaagc
     3421 taacataaca taatcaacta attcctgtat gcttgaaggt gcataacata atgttctgta
     3481 atcaccgttt ccgccgcctc ttgtagattg ataataatct gcttgtgaag gctgtatgct
     3541 tccaagtccg gggccgcctc tcatcatatt aactataaca catggaatct cagctcctgc
     3601 agcgtaagta ataccttctt gttttaaaga tattccgggg cttgaagatg cagtcataca
     3661 tcttgctccc gctcctcctg ctccataaac catattaatt gcagctattt cactttctgc
     3721 ctgcagaaat actccccctg cttcaggtaa ctttcttgaa atatattcag ggatttcact
     3781 tgatggtgtt atgggataac cgaagaaaca tttgcagccc gccttaatgg cagcagcacc
     3841 tacagcttca ttgcctttca ttaatacttt tgacataatt aacctccatt ataatctttc
     3901 tactgttatt accgaatcgg gacacataat tgcacagtta ccgcaagcta tacatttttc
     3961 ttgatcagtt acgtgtgcag gactgtaacc cttattattt attttttcct tatttaattc
     4021 aataattttt accggacaaa ccgcaacaca aagctcacaa cccttgcaag catcgtcatt
     4081 aaatgtaacc ttacctttcg ccattataaa acctccttta actcatccat tcttccctca
     4141 tataaagatt tatggggaat aatttttcta ttatttcatg gttctttatt ttatcagctg
     4201 tctcttttat aacagcttca tatcttatgg gaatatctgt ttcccatgat accttttttg
     4261 taagctcatg accgaattcc acatcttcta ctgtagttgc tttaagcatg tgcgtattgt
     4321 tgactaatcc ggtaattttt aaccttgatg ttgcttcaat cattttaaga taattgatta
     4381 ctccatcaac agtgcttgta tcaggtctgt ttccgttaat cacataaaac atatcataat
     4441 cgacattttt tatttgttct gcgaatctgg caagaactct tgctcccaca gggtcgcctc
     4501 caacatcgat tatggattta atattcgggt ttgttataac tcccatcagt tcagccggca
     4561 atgcaggtaa atcgactgcc gtgtttctaa tacttgaatc gaataatttt atcccgattt
     4621 cttcaaaaaa ctctcttttt tctcttgatc ggaaataagg attaactata tctaaatcgg
     4681 caatactgac attctcgtat atttctttca ttttaacggc ataattaacc gcaaactctg
     4741 tcttgccgct gccgtagtgg cctataacaa taactaatct tttgtcatgc acaatatccc
     4801 tcctttcgga atatttatta tgtcttaatt ataggtttta gcctcctcaa caccctttaa
     4861 aactctcaat ccaccttcgg ccagagccaa aagctcgtcc tctcccggat agactataac
     4921 cttagctatg aattttactt tatcttcaat catcttgaca aaatccttgt cgtaagcaat
     4981 accgcctgtt attataatcg catcaacatc tccgtcaaga actactgcat attcgccgat
     5041 tgcctttgca acttgg
//
LOCUS       DAOSOW010000091         5000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_16934_length_5000_cov_4.019211, whole genome shotgun sequence.
ACCESSION   DAOSOW010000091 DAOSOW010000000
VERSION     DAOSOW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16934_length_5000_cov_4.019211"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..724)
                     /locus_tag="PLD67_03460"
     CDS             complement(113..724)
                     /locus_tag="PLD67_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOT21612.1"
                     /translation="MKSDVTMNQRINTKTITKVGILGAVATVLMLLEFPLWFAPSFYE
                     LDFSEVPVLLGAFALGPAAGAMIELVKILINFVLNGTDTGGVGEFANFIIGCAFIVPA
                     GFIYKRNKSFKTAILGLVVGTLTLALAGAFLNYYLLLPLYAKIYGYPIQAFIDMGNAL
                     NPAITDLKTFVLYAVVPFNLLKGTIVSAMVILIYKKLSPILHR"
     regulatory      complement(829..942)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1212..2135
                     /gene="spoIIIAA"
                     /locus_tag="PLD67_03465"
     CDS             1212..2135
                     /gene="spoIIIAA"
                     /locus_tag="PLD67_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HOT21613.1"
                     /translation="MLNLLNHLETRIKNIIIRDCGILDEIIEIRLRINSPVLIKTLKR
                     EFFLDESIVITKKDIDDILGNLTKNSIHAFENEINKGYITTEGGHRVGISGDCIYDKN
                     GLKGFKNITSLNIRIAKEFPGCSEKALKHFITPDKNIYNTLIIGPPLSGKTTLIRDVA
                     RNLSDGFKNPYFEGCDVTLIDERGEISAVYNGIPQMNTGRRTDVLSYCKKSDGFFMSI
                     RALSPKVIVSDELGSLEDFEIIQYALKSGIKVVATAHGFSLDDVKRNLYLKNIIDNNF
                     FERAFVLDKRPLKVNEVFDFQKNRVIYNDLD"
     gene            2122..2625
                     /locus_tag="PLD67_03470"
     CDS             2122..2625
                     /locus_tag="PLD67_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21614.1"
                     /translation="MIWIRIALFAGTVVTVKYIFSSINHDGDEKIKKMKELINFTEYL
                     RVYSCDMKMSIEEICLKYNYKSIETEVIIKSMINILKNKKSSKELLVNINKIMSTPET
                     FNAYFTEIIDYYGYTYSEVLDRKLKFTIEEMERAMKEFSIKHNEKKTLNNRISFLAGC
                     LAAIIMS"
     gene            2634..2831
                     /gene="spoIIIAC"
                     /locus_tag="PLD67_03475"
     CDS             2634..2831
                     /gene="spoIIIAC"
                     /locus_tag="PLD67_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HOT21615.1"
                     /translation="MDIDLIFKIGAIGIVVAVFNQILTKTGRDEQAMFVTLAGVIVVM
                     ALIVGMMNELFTEVKSVFNLY"
     gene            2852..3232
                     /locus_tag="PLD67_03480"
     CDS             2852..3232
                     /locus_tag="PLD67_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAC/SpoIIIAD family protein"
                     /protein_id="HOT21616.1"
                     /translation="MKIILIAVITLIMGITLSKFNSEFKVYITVIFGIVVIFLLFKEL
                     RVYLEEIVSLFVKYDIKTEYFGTILKIVGIAYICDFISLLCKDLDYESVGKKVEIAGK
                     LIILIYSIDVIKIFLDQILILVNG"
     gene            3237..4370
                     /locus_tag="PLD67_03485"
     CDS             3237..4370
                     /locus_tag="PLD67_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21617.1"
                     /translation="MKKITLFIIVFTVLFTFTCKASTMDEAMDEIPTKGIEDYINEKN
                     AYFRENNIRIRDLIRNAFTGNLDVSLKDYLYYELKKEQSFFKDILKTGINVFIICIAL
                     TIINYFTDEDTGKSVSDIVVLFSVIIIFTIILKDVLSIKSILKSDFTDFKTITEEINA
                     IFMAAMLTFGKLSLLQFFQTSLNYTIGITTQFIYSFIDIMTVIMIAVILTNNMNELIN
                     AKLLYKALKKGTLLILTGFMIIVVINFSVQGYILYKTDNIFISSLKAMSPASLPVVGN
                     AVTSFFGVFLKSLLMIKDVLGIVVIVFILSSFGGSIIKISIALIIYKVLGVVTEPFNE
                     NISNLIYEMADIFYIYLICLITPLVIVTVYYSIIINYMNNILG"
     gene            4529..4816
                     /locus_tag="PLD67_03490"
     CDS             4529..4816
                     /locus_tag="PLD67_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21618.1"
                     /translation="MNVKLPVTTEVPAFQREVNVKSISPEIIESINKENFQGEVVSNI
                     ILRFQEDMQISAIVNLRKMIGKDELDRLKSNIAEIFQLSSDKIEINGGAYE"
BASE COUNT         1808 a          701 c          849 g         1642 t
ORIGIN      
        1 ctctgcggtc aaaaaataat cagcttttat tagagaaata caaaaaaaga cgcattttat
       61 atgcgtctgt ggcaattgat taattacgac attttcctgc atcgatcttc aattatctgt
      121 gcagtattgg agaaagcttc ttataaatca atattaccat agccgaaact attgtgcctt
      181 ttagtaggtt aaagggtact acggcataca gtacaaatgt ttttaaatct gttattgccg
      241 gatttaaagc attacccata tctataaatg cctggattgg atagccgtag atttttgcat
      301 atagcggaag caacagataa taattcaaaa atgctcctgc taatgctaag gtcaatgttc
      361 cgacaactaa tcccaaaata gcggttttaa aacttttatt tcttttataa atgaagcctg
      421 caggtacaat gaatgcgcat cctattataa agttagcgaa ttctcctact ccaccggtat
      481 ctgttccgtt taaaacaaag ttaattaaaa tcttaaccaa ttctatcatg gcgcctgctg
      541 ccggacctaa ggcaaatgct cctaataata ccggtacttc actgaagtct aattcataaa
      601 agcttggtgc gaaccatagc ggaaactcta aaagcattaa tactgtcgct acggctccta
      661 atattcctac ctttgtaata gtttttgtgt taattctttg attcattgtt acgtcacttt
      721 tcatattttc ctccttgtgc tcagcacgat tttaagattg tcagaggcaa ataaaaatcc
      781 tctgaaatct tcagaggcat tttaaagaat ttgcttacaa gatgcaagta atctcttcta
      841 ccatccagac tttactgtcg gtattggaat ctcaccaatt caacctttcg gctcgcggac
      901 tttaccgccg gtcgggaatt tcaccctgcc ctgaagatat ttacatgact attataccat
      961 taattatata tttgtaaacc tcttttttaa aaaaatttga tatttttttg gcgaatctta
     1021 taaaaacatc ctctttcgct caggatgaca aagtgattca tttaacgtgt tatggtaaac
     1081 atgtggtgat tctgaacgaa gctataaatc taaaaatcag ttttaaagga atgtcagcag
     1141 aagaaacaaa ataagtcata aatttttaaa ttaataatat tatagattaa gaggacaagg
     1201 taggagagaa catgttaaat ttattgaacc atttagaaac aagaataaaa aatattatta
     1261 ttagagattg cggtattttg gatgaaatca ttgaaatcag gcttagaatt aacagtccgg
     1321 tacttattaa aactctaaaa agagagtttt ttttagatga aagtattgtt attactaaaa
     1381 aagatatcga tgatattttg ggaaacttaa ccaaaaattc aatacatgca tttgaaaacg
     1441 aaataaacaa gggctacata accacagaag gaggacatag ggttggtata agcggagact
     1501 gcatctatga caaaaatggg cttaagggat ttaaaaacat tacttctctt aacattagaa
     1561 ttgcaaaaga atttccggga tgctccgaaa aagcattaaa gcatttcata actccggata
     1621 aaaatattta caatacattg attataggac cgccgctttc agggaagaca acattaataa
     1681 gagacgtagc aagaaattta agcgacggct ttaaaaaccc gtattttgaa ggttgtgacg
     1741 taactttaat cgatgaaaga ggagaaataa gcgccgttta taacggtatt ccacaaatga
     1801 atacaggcag aaggacagat gttttgtcct actgcaaaaa aagtgacgga tttttcatga
     1861 gtataagggc actttctccc aaggtaatag tttctgatga attaggctct ctagaggatt
     1921 ttgaaattat acaatatgca ctgaaaagcg gaataaaagt tgtggcaact gcacacgggt
     1981 ttagtttgga tgatgtgaaa agaaatttat acctaaaaaa tataatagat aacaatttct
     2041 ttgaaagagc atttgtttta gacaaacgac ccttaaaagt aaatgaagta tttgattttc
     2101 aaaagaacag ggtgatttac aatgatttgg attaggattg cactatttgc aggcacagta
     2161 gttaccgtta agtatatatt cagctcaata aaccatgatg gtgatgaaaa aataaaaaaa
     2221 atgaaggaat taattaattt cacggaatat ctcagggtat attcgtgtga tatgaaaatg
     2281 tctatagaag aaatatgctt aaaatataat tataaaagca tagaaacaga agtgataata
     2341 aagtctatga taaatatttt gaaaaacaaa aaaagctcaa aagagctttt ggttaacata
     2401 aataaaatta tgtcaactcc ggagaccttt aatgcatatt ttacggaaat aattgattac
     2461 tatggttata catattcgga agtattggac agaaagctta aatttaccat cgaagaaatg
     2521 gaaagggcta tgaaagaatt ttctattaag cacaatgaga aaaaaacatt aaacaacaga
     2581 atttcatttc ttgctggttg tctggcagct atcataatgt cttaggaggg aatatggata
     2641 tagatttaat ttttaaaata ggcgcaatag gaattgtagt tgcggtattt aatcagattt
     2701 taacaaagac gggaagagat gaacaagcca tgtttgttac cttagcggga gttattgttg
     2761 taatggcttt gattgtggga atgatgaatg aactgtttac ggaggtaaaa tctgtattta
     2821 acttatattg aagagggata tcatgctttt gatgaaaata attcttatag ctgtaattac
     2881 attaattatg ggaataactt tatccaaatt caactccgaa ttcaaagtct acataacagt
     2941 tattttcggt attgttgtaa ttttcctgct ttttaaagag cttagagttt atttggaaga
     3001 aatagtaagt ttatttgtaa aatatgatat aaagactgaa tatttcggta ccatattaaa
     3061 aattgtcggt atagcttaca tttgtgactt catatcactt ttatgcaagg atttagatta
     3121 tgaatctgtg gggaaaaagg ttgaaattgc cggaaagctt ataatactca tctattccat
     3181 cgatgttata aaaatatttt tagatcaaat tttgatctta gttaacgggt gataaaatga
     3241 aaaaaataac cttatttata attgtgttta ccgtattatt tacctttacg tgcaaagcct
     3301 caaccatgga cgaagcaatg gatgaaattc ctactaaggg tatagaagat tatataaacg
     3361 aaaaaaatgc gtatttcaga gaaaataaca tacgtatacg agacttgata agaaacgctt
     3421 ttacaggaaa cttagatgtg agcttgaagg attatttgta ctatgagtta aaaaaagaac
     3481 aaagtttttt caaagatatt ttgaaaaccg gtatcaatgt gtttatcatt tgcatagctc
     3541 taaccataat aaactatttt accgatgaag ataccggaaa aagtgtttcg gacatagttg
     3601 tattattttc ggtaattatt atttttacta tcatacttaa ggatgtactc agcattaaaa
     3661 gtatacttaa aagtgatttt actgatttta aaactattac ggaagaaatc aatgcaatat
     3721 ttatggcagc aatgcttaca ttcggtaagt tgagtttgct gcagttcttt caaacttccc
     3781 ttaactatac aatagggatt acaactcagt ttatttacag cttcatcgat attatgacgg
     3841 taataatgat agctgttata ttaaccaaca acatgaatga gcttattaat gccaagcttt
     3901 tgtacaaggc attaaaaaaa ggaacccttt taattttaac cggatttatg attattgtag
     3961 tcataaattt ttctgtacaa ggatatatat tatataaaac agataatatt tttataagtt
     4021 cattaaaggc aatgtctccg gcttcattgc ctgttgtggg aaatgcagta acatctttct
     4081 tcggtgtttt tttaaaaagc ttattgatga taaaagatgt acttggaatt gtagtaatag
     4141 tttttattct gtcatcattt ggcggctcaa taataaagat tagtatagca cttattatat
     4201 acaaagtatt aggggtagtg actgaacctt ttaatgaaaa tatatccaat ctgatttatg
     4261 aaatggcgga tatattctat atttacctta tatgccttat tactccctta gtaattgtca
     4321 ctgtttatta ctcaataata ataaattata tgaacaacat attaggataa ctatgaaaac
     4381 aacgattgtt aataccttga tattaatact tatttttaat ttaataatga taattttccc
     4441 ggagggaaaa acacagaaat tttgcagaat atctattaaa cttttaatca ttattttcat
     4501 tctggataat atatttctta atgggagcat gaatgtaaaa ttgccggtaa caaccgaagt
     4561 tcctgccttt caaagagagg taaacgtaaa aagcatttct cccgaaatta ttgaatcaat
     4621 taataaagag aatttccaag gagaagttgt ttcaaatatt atcttaagat ttcaagagga
     4681 catgcaaata tcagccatag tcaacctaag aaagatgatt ggaaaagatg agctcgatag
     4741 attgaaatca aacatagctg aaatttttca acttagctca gataagatag aaataaacgg
     4801 aggggcttat gaatgaattt tttaaaatgc taaaagaaaa caaaaaatta agctatgcag
     4861 ttaatgtttt catattgctg ttgctttaag caaacctgtc agtttttgaa acctaacagg
     4921 tttatcagag taactaattt cttagtcaaa cataaaacta attatttagt tgtacaacta
     4981 tttttttagt taaacaattg
//
LOCUS       DAOSOW010000092         4976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_17037_length_4976_cov_4.515342, whole genome shotgun sequence.
ACCESSION   DAOSOW010000092 DAOSOW010000000
VERSION     DAOSOW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17037_length_4976_cov_4.515342"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="PLD67_03495"
     CDS             complement(<1..347)
                     /locus_tag="PLD67_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOT21619.1"
                     /translation="MPYKPRRPCAYPGCSRLATSEQYCAEHKKLVNKHYNQYERDPDS
                     NKRYGRAWKRIRDRYIKAHPLCEECEKEGMLTPAEEVHHILPLSKGGSNNQDNLMSLC
                     KSCHSSITARDGDR"
     gene            complement(433..696)
                     /locus_tag="PLD67_03500"
     CDS             complement(433..696)
                     /locus_tag="PLD67_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21620.1"
                     /translation="MSEWQALYFTKDPLIITNFIKVYVGDEDITSRIDKLQVTKVDDE
                     YFVSAYKENNLVEIIPEGVKVKNIMLDKGYIGFSGGITYKIEI"
     gene            complement(800..931)
                     /locus_tag="PLD67_03505"
                     /pseudo
     CDS             complement(800..931)
                     /locus_tag="PLD67_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(1052..1468)
                     /locus_tag="PLD67_03510"
     CDS             complement(1052..1468)
                     /locus_tag="PLD67_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HOT21621.1"
                     /translation="MTAKEYLGQAYRLDQRINSKLEQVASLSDLATKVTTTISDVPKN
                     PNHSTSTMADVIVKIVDLQAEINHDIDCLVDLKREIVKVIKTVDNIEYQTLLELRYLC
                     FKTWEQIAVDMGYNVRHVYRVHDLAVSTIKITKRSQ"
     gene            complement(1465..1686)
                     /locus_tag="PLD67_03515"
     CDS             complement(1465..1686)
                     /locus_tag="PLD67_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21622.1"
                     /translation="MNDPYESLANAIILQAAKDYRNALKRLKKHPNREIDLYMKQEVE
                     QFFRSDWYSCLTTVDPEILIRKLNEEVIS"
     gene            complement(1679..3048)
                     /locus_tag="PLD67_03520"
                     /pseudo
     CDS             complement(1679..3048)
                     /locus_tag="PLD67_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237026.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(3045..3335)
                     /locus_tag="PLD67_03525"
     CDS             complement(3045..3335)
                     /locus_tag="PLD67_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HOT21623.1"
                     /translation="MMEKSIEQKLVAKVKKLGGICPKFISPGYDGMPDRLVLLPKGKI
                     GFVEVKKKGMKPRPLQLARHKLLKGLGFKVFVLDDEKDIDEIVENILGGDAR"
     gene            complement(3504..>4976)
                     /locus_tag="PLD67_03530"
     CDS             complement(3504..>4976)
                     /locus_tag="PLD67_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HOT21624.1"
                     /translation="EYGAELKLKPEDFSDVGQAIVLSSEYKVVLRYSPATDYIVYNGS
                     FWEESKPKAQGIAQELTTNQLEEAELEIEHALDEMTSNGTLDIVMENGAKKAVGHFND
                     EQEKSFKRYENAMKYKKYVIGRRDSKKISSSLKEAQPMLEIEPMDLDANGFLLNTPTA
                     TFDLKKGLSHSTKPNSSHLITKQTSVSPSNEGMDKWIDAVNVFFCKDKDLIEYVQRIV
                     GLAAIGKVYVEALIIAYGEGRNGKSTFWNAISRVLGSYSGNISADILTVGCRRNVKPE
                     LAEAKGKRLLIASELEEGMRLNTSNIKQLCSTDEIYAEKKYKAPFSYVPTHTLVLYTN
                     HLPKVGAIDEGTWRRLIVIPFEAKIEGSKDIKNYTDYLVKNAGGAILTWIIEGAEKVI
                     NENYNLKSPKKVKDAIKNYRENNNWLSHFLSECCETDKSYTAKSGEIYDEYRAFCLRV
                     GEFTRSTTDFYTALESHEFERKKTSKGIIVKGLRLKSDFL"
BASE COUNT         1392 a          952 c          791 g         1841 t
ORIGIN      
        1 cggtcaccat ctcttgcagt tatagatgag tgacaggact tacaaagaga cattaagtta
       61 tcttggttgt tgctaccacc ttttgagagg gggagtatat ggtgtacttc ttctgcaggg
      121 gtaagcattc cttctttttc gcactcctca catagagggt gggctttaat gtacctatct
      181 cttattcgtt tccaagcccg accgtatcgt ttgttggaat cagggtcacg ttcatattgg
      241 ttataatgtt tattcactaa cttcttatgt tcggcacagt attgctcact tgtagcaagc
      301 cgactacatc cggggtaagc acagggacgt cttggtttat atggcattaa gtcacctcct
      361 tttgggcata gaaaaacccc aaagaattgt tccttggggt aaaccatttt attatgaaac
      421 agatagattt aattaaattt caattttata tgttattccg cctgaaaagc ctatatatcc
      481 tttatctaac atgatgttct taactttaac tccttcagga attatttcca ccaaattgtt
      541 ttctttatat gctgacacaa aatattcatc atcaactttt gttacttgta acttatctat
      601 tcttgatgta atatcttcat caccaacata tactttaata aaatttgtaa ttattaaagg
      661 gtctttagta aaatataatg cttgccattc agacattaat taccaccttc tataattttt
      721 tatattgata gttgacacac tttcattaat gccattatat cacatataaa gatgtattca
      781 agtgaactaa tgtgcattat ttggaagccg ggtcacgctc atacttattg tagtttttat
      841 ttactatact cttatgtctt tcacagtaca tattatccgt tagttcagga cagccagggt
      901 agaaacaggg gcgtttaggt ttcttaggca tagaacacct ctttccagac ataacaaaag
      961 ccaccgcaga tttgtttctc tgcaatggct ttttaagtct atttttctat tctaataata
     1021 tcacacttga catagtgaca aacagtgaca tttactgact tcttttagta attttaattg
     1081 ttgagacagc taaatcgtga acacgatata catgacgcac attatatccc atatctacag
     1141 ctatctgctc ccaagtttta aagcataggt atcgcagctc taaaagtgtt tgatattcta
     1201 tattgtctac ggtctttata acttttacaa tttcacgttt caaatcaaca agacagtcga
     1261 tgtcatgatt tatttctgcc tgcagatcaa ctatttttac aattacatct gccatggttg
     1321 atgttgaatg gtttggattc tttggaacat ctgagattgt agttgttact ttggttgcga
     1381 ggtcgcttag tgaagccact tgctcaagtt tactattgat gcgttggtca aggcgataag
     1441 cctgaccgag atattctttt gctgtcatga tataacctcc tcgttaagtt tgcggataag
     1501 tatctccgga tcaactgtag taaggcatga gtaccaatcg gaacggaaaa attgctcgac
     1561 ttcctgtttc atatataaat caatttctct attagggtgt tttttaagtc tttttaatgc
     1621 attacggtaa tcttttgctg cctgcaaaat aatggcattc gccaagcttt cataagggtc
     1681 attcatagat tttacctcct gtttgtaggt ttacttttac tgcatcaatt aatgcagctt
     1741 gtgtgttatc tttattttta agtgccttca ttacacgttc atcaatcgtg cctttagaaa
     1801 ttatgtgatg aataacaacc gtgtcttttt gtccttgtct ccaaagtctt gcattagttt
     1861 gttggtatag ttccaaactc caagtaagac caaaccagat aagagtagag ccaccagttt
     1921 gtagattaag tccatgtccg gcagaggcgg ggtggatgat ggcaacagga atttcaccct
     1981 tattccatct tttaattgag tcagcactat ctaaggtttc aactgaaaac ctttcagaaa
     2041 tacgcttcat atcatgttta aaccagtaag atattaaaac aggtttgccg ttagcagatt
     2101 ctattaagtc ctctaaagca tcaagcttac gttcatgtat attaatcact tcaccagcct
     2161 caccataaac tgcgccgttt gccatttgta aaagtttagt tgataaagca gcagcattgg
     2221 atgcagtaat ttcatcatct tttattttag ttactaattc ttttttcata tcatcaataa
     2281 ttttcatttc tttttcagac agattaactt gtacttcatt tattacgagt tcaggaagtt
     2341 taatataatc agaacctttc atactgatgg ttatatctga aatcttttta tagatttctt
     2401 cttcagctcc tggcttaggc ttgtatgaaa aaataacttg ctggtttctt ttatcgggta
     2461 caaagtagtc gtctctgtat ttaccaataa atcttccaat cctttgcccc atatctaaaa
     2521 gtctaaattc agcccatagg tcaattaaac cgttgcttgc aggtgttccg gttaaaccaa
     2581 caattctttt aatctttggt cggactttca tcaaagaacg gaagcgttta gcctgatgat
     2641 ttttaaatga tgaaagctcg tctatgatta atgtgtcaaa gtcgaagggg agagagctgt
     2701 cttccacaag ccatttaaca ttttcacggt ttataatata aatatcagct tttatcataa
     2761 atgcagcttt tctttccttt tcacttccaa gagcaacaga atattttaaa tcttttaaat
     2821 gatcccattt ttcaatttct gaaggccatg tatctctggc aactcttaag ggtgcaatta
     2881 ttaagatttt gtgtatttca aaactatcaa acaagagatt gtttaaggct gttaaggtca
     2941 agacagtttt tcctaaccta agcccatatc caagaatatt gctgatattg agtgcttttc
     3001 tatatatgca cttgcatatc gctgataatc gtggggtatg aacttcatct ggcatcacct
     3061 cccaatatat tttcaactat ttcatctata tctttttcat catctaaaac aaatacttta
     3121 aatccaagcc ctttaagaag tttatgtctt gctaattgaa ggggacgggg tttcattcct
     3181 ttttttttaa cctccacaaa tcctattttt ccttttggca gtaatacaag gcggtcaggc
     3241 atgccatcat agccggggga gataaacttc ggacaaatgc cacctaattt tttaactttt
     3301 gctacaagtt tttgctctat tgatttttcc atcatcgtaa aaaacctcca tttttcaaca
     3361 ttctcttaaa aaagtgcagg tctgtgcatg tcatgtatat aactatatat atatttattt
     3421 tttatataat ttttttatat gtaaaagttt tatatatgac cttcatagac ctgcactatg
     3481 tgtaaatttc aacgtttctt tgattataga aagtcggatt ttaatcgtaa tcctttaaca
     3541 atgatgcctt ttgatgtttt ctttctttca aactcatgag actccaatgc tgtataaaaa
     3601 tcagtcgtgc ttcttgtaaa ctctccaact cttaagcaaa atgcacggta ttcatcgtaa
     3661 atttcacctg atttggctgt atagctttta tctgtttcac agcattctga aagaaaatgc
     3721 gagagccagt tgttattttc tctataattt ttgatggcgt ctttgacttt tttaggagat
     3781 tttaaattgt aattttcatt aattactttt tctgctcctt cgattatcca tgtaagtatt
     3841 gcaccgcctg catttttaac gaggtaatct gtataatttt taatatcttt acttccttcg
     3901 atttttgctt caaaggggat tacgataagc cttctccaag ttccttcatc aattgcacca
     3961 acctttggca ggtgattagt gtataaaact aaggtatgag taggtacata gctaaagggc
     4021 gccttatatt ttttctctgc gtatatttca tcagtggagc agagctgctt aatatttgaa
     4081 gtattaagtc tcataccttc ttctaactct gaagcaataa ggagtctttt tcctttagct
     4141 tcagcaagtt caggttttac atttctcctg caaccaacag taaggatgtc tgcagatata
     4201 ttgccgctgt atgaacctaa aactcttgat atggcattcc aaaatgttga tttaccatta
     4261 cgcccttcac cataagcaat tattaaagct tcaacataaa ccttacctat tgcagcaagc
     4321 cctacaattc tttgcacata ttcgatcaag tctttatctt tacagaaaaa cacattaact
     4381 gcatctatcc atttatccat tccctcatta cttggactta ccgatgtttg ttttgttatt
     4441 aaatgagaag aattcggttt tgtactatga cttagccctt tttttaaatc gaaggtggct
     4501 gttggtgtgt ttaataaaaa tccattggca tcaaggtcca taggttcaat ttcaagcata
     4561 ggttgtgctt cttttaagga tgaagatatc tttttggaat ctcttctgcc tatcacatat
     4621 ttcttatatt tcattgcatt ctcatatctt ttaaatgatt tttcctgctc atcattgaaa
     4681 tgacctactg cctttttagc accattttcc ataacgatat ctaaagtacc attactggtc
     4741 atttcatcta aagcatgttc tatctcaagt tcagcttctt caagttgatt agttgttagc
     4801 tcctgcgcta tcccttgtgc ttttggtttt gattcctccc aaaagctgcc gttatataca
     4861 atgtaatctg tagcagggga gtaccttagc actactttat attcacttga aagaactatt
     4921 gcttgcccta catcagaaaa atcttcaggc tttaatttaa gttctgcacc atattc
//
LOCUS       DAOSOW010000093         4951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_17146_length_4951_cov_4.070670, whole genome shotgun sequence.
ACCESSION   DAOSOW010000093 DAOSOW010000000
VERSION     DAOSOW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17146_length_4951_cov_4.070670"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..182
                     /locus_tag="PLD67_03535"
                     /pseudo
     CDS             30..182
                     /locus_tag="PLD67_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009794012.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
     gene            complement(361..690)
                     /gene="trxA"
                     /locus_tag="PLD67_03540"
     CDS             complement(361..690)
                     /gene="trxA"
                     /locus_tag="PLD67_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT21625.1"
                     /translation="MAENIKIINSTQEFDDMIKQNRYVLAEFWAEWCTPCRMVAPVIE
                     KVAEQYSGKINVAKVDIDEQQELAIRYGIQTIPTIIFFKEGKFASKEIGVKPLASFAR
                     MIDSNIA"
     gene            complement(674..1549)
                     /locus_tag="PLD67_03545"
     CDS             complement(674..1549)
                     /locus_tag="PLD67_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003532471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT21626.1"
                     /translation="MGDIVDIIIIGAGPAGLSAAVNAVTRGKTVRIFSNKENNLSKAE
                     RVDNHLGFYHASGKELMDKFKEHAKAMDIKIEEGMAVNILPLGESFMVNVNGEIFESK
                     KVILAMGITKVKELPGEGRLLGNGVSYCATCDGMLYRGKNALVWDQSKDAWEEANFLQ
                     SIGVNVTFISKKPKQENLSNDIKFIEGTITEIIGENTVEAVNTGNDLIKTDAVFMLRD
                     AVAPAALIDGLELDGNFINTNKHMETNIPGLYAAGDITGKPLQLSKAIGEGLIAAQHA
                     ALQIDKKENLNGRKY"
     gene            complement(1700..2425)
                     /locus_tag="PLD67_03550"
     CDS             complement(1700..2425)
                     /locus_tag="PLD67_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HOT21627.1"
                     /translation="MGGAGLSTNVSIILVFAEGFLSFFSPCVLPLIPVYISYLAGNGR
                     KISSDGTITFERKKVLFNTLFFILGISSVFFILGLSFSTLGIFFNKNRELFSKIGGII
                     IVIMGFVQLNIIKLNFLKVEKRINLNIGGKINPILAYIMGFTFSFAWTPCVGPALASV
                     LILASNATNAFAGNMLVFVYALGFIIPFIILGVFTTEALNFFKEKRQLVKYSIKIGGI
                     ILIIIGIMTFTGIFTAISRNLSF"
     gene            complement(2595..>4951)
                     /locus_tag="PLD67_03555"
     CDS             complement(2595..>4951)
                     /locus_tag="PLD67_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HOT21628.1"
                     /translation="FLLTVIIILFNGFLALKDSIKNVSEKFEDIQQFPRKQIDLNNIL
                     NKKFVERIINQLSIIGVFLFLLLAVRYVLRSYDLLFSRLGRVFGAGYTDINVTLNLYR
                     ILAVGCVLAAITFFIGARKRNLKTALAVPAALIIISIAGTGVAGAVEKFVVEPDQLSK
                     ETKYMQYSIKSTQNAYSLNNVKTIQFPANNNLTIEDLQNNPEVIDNIRINDQEPLIQV
                     YNQLQGIRPYYVFNDVDVDRYVIDGDYKQVFLSARELDQDRLNEQARTWVNQYLKYTH
                     GFGITVSTVNNVTPQGQPEILVKNIPPTTETDFNIQRPEIYFGEKTNNYVIVNTDEME
                     FDYPSGADNVETIYEGKAGINLSFFNRLMFSIRKGSYRMMISNNIDSNSKILINRNIM
                     QRVTEIAPFMYYDPDAYIVVNQDDGKLYWIIEGFTVSDRFPYSQPTDIFIKGMDVNYI
                     RNSVKVVIDAYDGTIDFYVADENDPIIKTYDKIFTDLLKPIDEMPEGIRKHIRYSRAF
                     FDVQSDMYRLYHIENVTVFFGREDYWDLANEKYMGGGEVPAGSSYLMFKLPGEENVEF
                     LLTNQYTPQNKDNMIALLAARNDGENYGELVLYEFPKTKVIPGPNMIETKIDQDTNIS
                     SQLTLWSQLGSDVLRGNTLVIPIEESLLYVEPIYLKSDTDSNFPEMKMVVVSYGDKIL
                     MEPTLDSAINRLFGISEQEPGRPQVPDEEYDNTNINDLIIKANEVFNDANEASQNGNW
                     AEYGRKINELERLLNQLNALINGQQEQEARDENVQEESSGMPSE"
BASE COUNT         1459 a          976 c          764 g         1752 t
ORIGIN      
        1 accgttaggc ggcatgctcc gacacataga aaacaattgc cttatatgaa aaatgcggtt
       61 tcaaaaaaga aggtattttc agagaatttg tattaagaga cggtaaaaga tataatttgc
      121 ttttgtatgg tttattaaga agtgaatggg aaaaaactga gatccttcgc ttacgctcgt
      181 gatgacaatc acagaccata ttcctttatc gcggtaagat aaactcgtga caattataag
      241 ggatagacta aagaatctca gattttcagg gtatgaggac acacctccac tcttaggata
      301 gtttccgaag taagaggaat gtcctcatac atgagaatct tcgctatgct aaggatgata
      361 ttatgcaata ttgctgtcaa tcattcttgc aaaagaagct aagggtttta ctccgatttc
      421 ttttgaggca aactttcctt ccttaaaaaa tattatagtc ggtatagttt gtattccgta
      481 gcgaatagct aattcctgct gctcatcaat atctaccttt gcaacattaa ttttcccgct
      541 atattgttca gctacttttt ctattaccgg tgcaaccatt ctgcaaggag tacaccattc
      601 tgcccagaat tccgccaata catatctgtt ttgtttaatc atgtcgtcga attcctgagt
      661 actgttgata attttaatat tttctgccat ttaaattctc ctttttatct atttgtagtg
      721 ctgcatgctg agcagctatc aaaccttcac ctattgcttt tgaaagctga aggggttttc
      781 ctgtaatgtc tcctgccgca tataatcccg gaatattggt ttccatgtgt ttattggtat
      841 ttataaaatt accgtcaagt tccaaaccat caatcaatgc agcaggtgca accgcatctc
      901 ttagcataaa tacggcatcg gtttttataa gatcatttcc agtattaaca gcttcaacag
      961 tgttttcgcc tattatttcg gtaatggttc cttcaatgaa ttttatatca ttgcttaaat
     1021 tttcttgttt tggttttttt gatataaagg ttacattcac tccgatactt tgcaaaaaat
     1081 ttgcttcttc ccatgcatct tttgattggt cccatacaag agcattttta cctctgtaaa
     1141 gcattccgtc acatgtagcg cagtaactca ctccgttgcc taagagtctt ccttctcccg
     1201 gcaattcttt gacttttgtt attcccattg ccaatattac ttttttggat tcaaaaattt
     1261 caccgtttac attgaccata aaactttcac ctaatggaag tatgtttacg gccatacctt
     1321 cctctatttt tatatccata gcctttgcat gttctttaaa cttatccatc aattcttttc
     1381 cgctggcatg ataaaaacct aaatgattat caacacgctc ggcttttgac agattatttt
     1441 ctttatttga aaaaattcgt acagtcttgc ccctggtaac agcatttact gctgcggata
     1501 aaccggcagg accggctcct ataatgataa tatcaactat atctcccatt ctaacctctc
     1561 tttcttcaaa atattacagt ctcacgcagt ctcattcacc aatttattag ctcctttgtc
     1621 gcataaattg ggaattcgtt gcgaatgacc taccgctgtt ttttcttcgc tttggctcga
     1681 aaaaacaggg ttaggacatc taaaagctta ggtttctgct tattgcagtg aatattcccg
     1741 taaatgtcat aattcctatt attattaaaa taataccgcc aatctttatt gaatatttga
     1801 ccagttgtct tttttcttta aaaaaattca atgcttcggt ggtaaatact cccaatatta
     1861 taaagggtat tataaatcct aaggcataaa caaataccaa catgtttccg gcaaatgcat
     1921 tggtggcatt tgatgccaga attaagactg aagccagtgc aggacctacg cagggagtcc
     1981 atgcaaaact gaatgtaaat cccattatgt atgcaagtat tggatttatt ttcccaccga
     2041 tatttagatt tatccttttt tcaactttca aaaaatttaa ctttattatg ttaagttgaa
     2101 caaaacccat tattactatt attatgccgc caattttact aaataactct ctgttcttat
     2161 taaagaatat acccaaagtt gaaaatgata aacccaaaat aaaaaatacg ctggaaatac
     2221 ctaatataaa aaataaagtg ttaaatagta ccttttttct ttcaaatgta atagtaccat
     2281 cgctgcttat ttttcttcca ttgcctgcca agtagctgat ataaacagga attaaaggaa
     2341 gaacgcatgg tgaaaaaaaa gataaaaacc cttcggcaaa aaccaatatt atacttacat
     2401 tagttgataa acctgcacct cccattttga cctcccgaaa taatattatt aaaatatagc
     2461 agggcaactt tactatgtca agtaaccttg tcacatatgt ttcaacagcc acggaccttg
     2521 cccgtggctg ttatttatct tattgatatt acttctatcc atttaaccaa ccgagtcagt
     2581 aatgaagtat catatcattc tgagggcatg cccgaagatt cttcctgtac attctcatct
     2641 cgagcttctt gttcttgctg gccgtttatt aaagcattaa gctggtttaa gagcctttcc
     2701 aattcattaa tttttctgcc gtattctgcc caatttccgt tttgtgaagc ttcatttgca
     2761 tcattgaaca cttcattggc tttaataatt aaatcattaa tatttgtatt atcatattct
     2821 tcgtcaggca cctgaggtct tccgggttct tgctcagata tgccgaaaag tctgtttatg
     2881 gctgaatcca gtgtaggctc catcaatatt ttatcgccat aggatacaac aaccattttc
     2941 atttcgggaa aattgctgtc tgtatctgat ttcaaatata tgggctctac atacagcaat
     3001 gattcttcta ttggaattac caacgtgttt cctctcaaaa catctgaacc caattgactc
     3061 cacaaggtaa gctgtgatga tatattcgta tcttggtcaa tttttgtttc tatcatatta
     3121 ggtccgggaa ttacttttgt ttttggaaat tcgtacagca caagttcacc gtaattttcg
     3181 ccgtcatttc gtgcagccaa aagtgctatc atattatctt tgttctgggg agtatattga
     3241 ttggtcaata aaaactccac attttcttct cctggaagtt taaacatcag ataacttgaa
     3301 cctgcaggta cttctcctcc acccatatat ttctcgtttg ctaaatccca ataatcctct
     3361 cgcccaaaga aaaccgttac attttcaatg tggtacaatc tgtacatatc tgattgtaca
     3421 tcgaaaaatg cacgtgaata tctaatatgc tttctaatac cttccggcat ttcatctatc
     3481 ggtttcaata aatccgtaaa aattttgtca taggttttta taattgggtc attttcatct
     3541 gccacataaa aatcaattgt gccgtcataa gcatctatta caacctttac ggaatttctt
     3601 atataattaa catccatgcc cttaataaat atgtctgtag gttgtgaata agggaatctg
     3661 tcgcttacgg taaatccctc aataatccaa tataattttc catcatcttg atttactaca
     3721 atatatgcat ccgggtcata gtacataaaa ggagctattt cagttaccct ttgcattata
     3781 tttctattta ttaaaatttt ggaattgctg tcaatattgt tggaaatcat cattctgtag
     3841 ctgccttttc ttatgctgaa cataagtctg ttgaaaaaag aaaggttgat tcctgcttta
     3901 ccttcgtata tggtttcaac attatcggca cctgaaggat aatcaaattc catttcgtct
     3961 gtgtttacaa ttacataatt attggttttt tcgccaaaat atatttcagg tctctgtata
     4021 ttaaagtctg tttccgttgt gggaggaata tttttaacca gtatttcagg ctgcccctgt
     4081 ggtgtaacat tattcacggt agataccgtt attccaaatc cgtgagtata ttttaaatat
     4141 tgattgaccc aggttcttgc ctgttcattt aatctgtctt ggtcaagttc tctcgctgac
     4201 aaaaaaacct gcttgtaatc gccgtcaatt acatatctgt ccacatctac atcattaaag
     4261 acatagtagg gtctgatacc ttgcagctgg ttataaacct gaattaacgg ctcctggtca
     4321 tttattctga tattgtctat tacttccggg ttgttttgta aatcttcaat ggttaaatta
     4381 ttattggcag gaaactgtat tgtttttaca ttgtttaaag aataagcatt ttgggtgctt
     4441 ttgatgctgt attgcatata ttttgtttct tttgacaatt ggtcaggctc aactacaaat
     4501 ttttctacgg cacctgccac gccggttcca gcaattgata tgataataag agctgcggga
     4561 actgccaatg ccgtttttaa atttcttttt cttgcgccga taaaaaatgt tatagctgca
     4621 agtacacatc cgacggctaa tattctgtat agattcaaag taacgttaat atcagtatat
     4681 cctgcgccga atacacggcc taaacgcgaa aaaagcaaat catagcttct taatacatat
     4741 ctcacagcta acagtaaaaa taaaaaaaca cctattattg aaagttgatt tattattctt
     4801 tcaacaaact ttttattcaa aatattattc aagtctatct gcttacgtgg aaattgctgt
     4861 atatcttcaa atttttccga aacatttttt atgctgtcct taagtgcaag aaagccgtta
     4921 aacaatatta taattactgt cagcaaaaat a
//
LOCUS       DAOSOW010000094         4887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_17424_length_4887_cov_3.456126, whole genome shotgun sequence.
ACCESSION   DAOSOW010000094 DAOSOW010000000
VERSION     DAOSOW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4887
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17424_length_4887_cov_3.456126"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3261)
                     /gene="addA"
                     /locus_tag="PLD67_03560"
     CDS             complement(<1..3261)
                     /gene="addA"
                     /locus_tag="PLD67_03560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HOT21629.1"
                     /translation="MNWTKEQKRIIDLKNSNILVSAAAGSGKTAVMVERIISLIKSGE
                     DIDRLLVVTFTKAAAAGMKQKIQKSLLNAYQNKDGNIKHLRKQLSLLNRSMITTIDSF
                     CLDVVKKNFHLIDIDPNFRVGDKSELSILLQDSIDEVLEEEYEKINGNENFRKLVEGF
                     TGNRGDDELSEIIQSTYRFILSFPDPFVWLSQSVDKLNIKGEELKNSSWLKEIKSYIM
                     LLLDGAKGYINTALEICNEPKGPRLYLDTLKKDMAIVDNLVNLLNNNMEEFMEAVKGF
                     KAPKAPPFKEKDNPDVDIEKQIEVGGSKNNNSLRAGYKSIIASIKELLPYDLDYDKYS
                     LEINEMYGSIAALKDLIIKTDKIYKEKKNEKSIADFNDLEHFALNILRSKNDKGVFEP
                     SEAAEYYRKKYNYIFIDEYQDSNSLQEAIIEQIKRENNLFMVGDVKQSIYRFRLADPS
                     IFNNKYTTYTMDREDLSNNIVDRTIDLNKNFRSRDEILKAVNFIFKNIMTKELGEVEY
                     NEKAALNTGSEFLTHNPVELNIIYKNTSLGNQEGEIEAGEVIKSEAEIEIESMETAEL
                     EALFAAKRIKELIKEETCIPGKTKGKKIEYKDIVILLRSVVNWAGIFEERFNKEDIPF
                     YYDGGKGYYDALEVRILVNLLRLIDNIRQDIPLLSVMRSPIGNFSTEELLEIRLKHPN
                     EKHFIDALNKYILCAKEEEESFPLADKLKNFKEKIYDWSYRSRYSHLHDLIWDILMES
                     DYYNFAGALPNGRMRQANLRMLADLAYDFEKTSMKGLFKFLRYIEKIDKGYDDRGQAK
                     TLGENDNVVRLMSIHKSKGLEFPVVILCGLNKQFNLQDTKNKILLHKDYGIAPMYVNV
                     NERFERETLARTAIKMRIKKENISEEMRVLYVAMTRAIDRLIMAGTVSNLEQKEKNWR
                     RGHSQYFVYKGLSYLDWICSCLFEKINFDYFNEIMAQGQWKNWILNLITRADLSLSIR
                     DEINFKESNIRAMNEYKDMKNSPYFEEIGRRLGFKYPYKASVNVPTKLSVTELKNLKG
                     KEYAKLRYKIPTLVDIFEYNEVTDKLIFNKEITGAEIGTLLHFVMEHLDLKGNLKKS"
     gene            complement(3276..>4887)
                     /locus_tag="PLD67_03565"
     CDS             complement(3276..>4887)
                     /locus_tag="PLD67_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOT21630.1"
                     /translation="RDILLENDVRLTKGADYDTFKEKHMLYKMFSSPSHSLSVSYPLG
                     LESGESLQPSLYIDILKKIFVSVKESSDLSISDELDLVSNYSGTYDYFVEKMRNFIDG
                     YETDNIWKDVYAWYEREEKEIFDIMVKGLNYKNAMNSLEPDIVKRIYENNMSMTVSKL
                     ESYAECQFKYFMENVLKPRERAYLKIEYYDLGNIYHSIVEKFINKISEDKEDIIILDR
                     TKAEDEAVKITDDVLLEQADKLTAIDANHRNKYMKEKIKRVMKRTCRTIVRQLNSSDF
                     RPKFTELQIDLVDENEEKAKKGVYLPPIEIPVETDKIKEVLKLRGKIDRVDVFENNDK
                     LYISIIDYKSSPNDIDLEDAQEGIQVQLIMYLKALLDKGEELFGKKPCIGGVFYYYIS
                     DPVYKDKNKEKEPEEEIFKSLKLKGYVLKDKEVIKYMDKNLGSTSSIIPAALKSDGEI
                     RDDRTKALTEESFNNLIDTVYNKCKDMTKSIISGNININPYKKIDGKTPCKYCEYISI
                     CQFDKTLGNNFRLIGNQFKNLSNLYTEH"
BASE COUNT         1412 a          981 c          578 g         1916 t
ORIGIN      
        1 ggattttttc aaattgcctt ttaaatcaag atgctccatt acaaaatgaa ggagtgttcc
       61 tatttctgca cctgtaattt ctttattgaa tattaattta tctgtaactt cattgtactc
      121 gaatatatca accaaggttg gaattttata tcttaatttc gcatattctt tacccttcaa
      181 atttttaagc tcggttacgg aaagctttgt aggcacattt accgaagcct tatatggata
      241 tttaaatccc aaacgccttc ctatttcttc aaaataagga ctgtttttca tatctttata
      301 ctcattcata gccctaatat tactttcctt aaaattgatt tcatccctta tacttaaact
      361 taaatctgcc cgtgtaataa gatttaaaat ccagtttttc cactgaccct gtgccataat
      421 ttcattaaaa taatcaaaat ttattttttc aaataaacag ctgcaaatcc aatccagata
      481 ggaaaggcct ttataaacaa aatattgtga gtggcctctt ctccaatttt tttctttttg
      541 ctccaaattg ctgaccgtcc ctgccattat taacctatct atagcccttg tcattgcaac
      601 atacagaact ctcatttctt ctgaaatatt ttcctttttt attctcattt taatggcagt
      661 tcttgccaat gtttctcttt caaatctttc attgacattt acatacattg gagcaattcc
      721 gtaatcctta tgcaacaata ttttattttt tgtatcctgc agattaaact gcttgttaag
      781 cccgcaaaga attactaccg ggaattccaa tcctttagat ttgtgaattg acataagcct
      841 aaccacattg tcgttttctc ccaatgtttt agcctgtccc ctgtcatcgt agcccttatc
      901 aattttttca atatatctaa ggaatttaaa caaacctttc attgatgttt tttcaaaatc
      961 atatgctaaa tcagccagca ttctcaaatt tgcctgcctc attcttccat ttggaagagc
     1021 tcccgcaaaa ttataataat cagattccat caaaatatcc catattaaat catgaagatg
     1081 agaatacctg cttctgtaac tccaatcata tattttttct ttaaaatttt ttaatttatc
     1141 agctaaggga aaagactcct cttcttcttt tgcgcataga atatatttat taagagcatc
     1201 tatgaaatgt ttttcattag gatgtttaag tcttatttcc aataattcct cagtagaaaa
     1261 attcccgata ggtgacctca taacgcttaa cagcgggata tcctgtctta tattatcaat
     1321 taatctaagt aaatttacaa gaattctaac ctctaaagca tcataatatc cctttcctcc
     1381 atcatagtaa aagggaatat cttctttgtt aaacctttct tcaaatatgc ctgcccaatt
     1441 tactacagac cttaataaaa ttacaatatc cttgtattct atctttttac cctttgtttt
     1501 tcccggaata catgtttctt cttttattaa ttcctttatt ctctttgctg caaacaaagc
     1561 ttccaattca gctgtttcca tagattcaat ttcgatttct gcttcacttt ttatcacttc
     1621 accggcttca atttctcctt cttggtttcc caaagaagta ttcttataaa taatattaag
     1681 ttcaacagga ttatgggtta aaaactcact tcccgtgttt aaagctgcct tctcattata
     1741 ttctacttcc cctaattcct ttgtcataat atttttgaaa atgaaattaa cagcttttaa
     1801 aatttcatct ctgcttctga aatttttgtt taaatcgatt gttctgtcta caatattatt
     1861 gcttaaatct tccctgtcca tggtgtaggt tgtatattta ttattgaata tactcgggtc
     1921 tgccaatctg aatctgtata tactctgttt aacatcacct accatgaaca aattattttc
     1981 tcttttgatt tgctcgatga ttgcttcctg caaactgttt gaatcctgat attcatcaat
     2041 aaatatataa ttatactttt tcctgtaata ttcggctgct tccgaaggct caaatactcc
     2101 cttgtcattt ttacttctta atatatttaa tgcaaaatgc tccaaatcat tgaaatctgc
     2161 tatggatttt tcattctttt tttccttgta aattttatca gtttttatta ttaaatcttt
     2221 caaagcggca attgagccgt acatttcatt aatttccaag gaatacttgt cataatccaa
     2281 atcataaggc aacaattcct ttattgaagc aataatactt ttatacccgg ctctcagact
     2341 gttattgttt ttagaaccgc ccacttcaat ttgcttttca atatctacgt caggattatc
     2401 tttttcttta aaaggcggag cttttggtgc tttaaatcct tttacggctt ccatgaactc
     2461 ttccatatta ttattaagaa gatttacaag gttatcaact atagccatat ccttttttaa
     2521 tgtatccaaa taaagtcttg gacccttagg ctcattgcat atttcaagag ctgtattgat
     2581 ataacccttt gcaccgtcca ataaaagcat aatataactt ttaatttctt taagccaact
     2641 tgagtttttc agttcctctc ctttaatatt aagcttatca acgctttgtg aaagccatac
     2701 aaaggggtct gggaagctta gaataaatct gtatgtactc tgaattattt cactaagctc
     2761 atcatcaccc ctgtttccgg taaatccttc aactaatttt ctgaaatttt cgtttccatt
     2821 aattttttca tattcttctt ccaaaacttc atctattgaa tcctgcaaaa gtatacttaa
     2881 ttcgcttttg tcgccaaccc tgaagtttgg gtcaatatca attaaatgga agtttttctt
     2941 cacaacatcc aggcaaaaag aatcgattgt agtaatcata gatctgttta agagggataa
     3001 ctgttttctt agatgcttta tattcccatc cttgttttga taagcattca ataatgattt
     3061 ttgaattttt tgcttcatcc ctgcagcagc cgctttagta aaggtaacta ccaaaagcct
     3121 gtctatatct tctccgcttt ttataagcga aataattctt tcaaccatta ctgcagtctt
     3181 ccctgaaccg gctgcggcag aaactaatat attagaattt tttaaatcaa ttatacgctt
     3241 ttgctctttt gtccaattca tctcttagcc cctccttagt gctcagtata aagattgctc
     3301 aaatttttga attgattacc gattaaacgg aagttattcc ctaatgtttt gtcaaactgg
     3361 catatactta tatattcaca gtatttacac ggagttttac cgtcaatttt tttgtaagga
     3421 tttatattta tatttcctga tataatactc ttagtcatat ctttacattt gttataaaca
     3481 gtatcgatta gattattaaa ggactcttcc gtcaaagctt ttgtccggtc atcgcgaatt
     3541 tctccatcgc tttttaaagc cgccggtatg atacttgaag tactgcctaa atttttatcc
     3601 atatacttga taacttcttt gtcttttaaa acatatcctt taagttttaa gctcttgaat
     3661 atttcttctt caggctcttt ttctttattc ttatccttgt atacagggtc gctgatataa
     3721 taataaaaca cacctcctat acaaggcttt ttaccgaaca gttcttcgcc tttatcaagc
     3781 aatgctttta aatacataat taattgtact tgaattcctt cttgagcatc ttctaagtct
     3841 atatcattgg gagaagattt gtaatcaata atactaatat ataatttgtc gttattttca
     3901 aaaacatcta ctctgtctat tttaccccta agttttaaca cttccttaat tttatccgtt
     3961 tccacaggta tttctatagg aggtaaataa actccctttt ttgctttttc ttcattctca
     4021 tctaccaaat caatttgaag ctctgtaaat tttggcctga agtcactcga attcaattgc
     4081 cgcacaattg tccggcatgt ccttttcatc actcttttta ttttttcctt catatacttg
     4141 tttctgtgat ttgcatcaat tgctgttaac ttatcagctt gctccaacaa aacatcatcg
     4201 gttattttta cggcttcatc ctctgctttt gtcctgtcaa gtataataat atcctcttta
     4261 tcttcactta ttttgttaat aaacttctcc actattgaat gataaatatt gcctaaatca
     4321 taatattcta tttttagata agctctttct ctcggtttta gcacattttc cataaagtat
     4381 ttaaattgac attcagcata gctttctaat ttagaaaccg tcatagacat attattttca
     4441 taaattcttt ttacaatatc aggctccaaa gaattcatgg catttttata atttaaaccc
     4501 ttaaccataa tatcgaaaat ttctttttct tccctttcat accatgcata aacatccttc
     4561 caaatattat ccgtttcata accatctata aaattcctca ttttttctac aaaataatca
     4621 taggttccac tgtagtttga caccagatcc aattcatcgg aaatgcttaa atccgagctt
     4681 tctttgacag aaacaaagat tttcttcaat atatcgatat ataatgatgg ctgcaaactt
     4741 tctccgcttt ctaagccgag aggataactg acggataaac tatgggaagg acttgaaaac
     4801 atcttgtaaa gcatatgttt ttctttaaag gtgtcataat ctgctccttt tgtaagtctt
     4861 acatcatttt caagaaggat atctctt
//
LOCUS       DAOSOW010000095         4863 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_17518_length_4863_cov_3.636647, whole genome shotgun sequence.
ACCESSION   DAOSOW010000095 DAOSOW010000000
VERSION     DAOSOW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17518_length_4863_cov_3.636647"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLD67_03570"
     CDS             <1..180
                     /locus_tag="PLD67_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOT21631.1"
                     /translation="DSLEDLIKKAMANGVKMIACTMSMDVMGITKEELIDGVEFAGVG
                     TYLGDAEESNVNLFI"
     gene            265..2331
                     /locus_tag="PLD67_03575"
     CDS             265..2331
                     /locus_tag="PLD67_03575"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT21632.1"
                     /translation="MVSSSLNKGLSIITYSKKHMENVIAQLTNLLGDEISIKGYSIQE
                     GINEEICDSLVLICHKYLKEEALKHITPGANVLVARRSLNHSKLEDILQIPEGEKVLF
                     VDVNKYLAESSISLLVELGINHISMYPYYPGQEFPEYADYSITTGETHLVPDYVKNII
                     DIGYSKIDLTTITEIMIKLGIMDERANLLSSRYLQDFAYSSKKIVETLSDNKHITEQL
                     NTILNVINDGIVGIDGSGEIMFSNNNANKLFNNTEINLNDINNFDIKSFQGKTFDVYR
                     EIHEIKGKKVLLTMMPIIEDNANNGIIITFKDLSEIMEMENIVRVNLFKKGHTAKYRF
                     SDIVGKSKNMSETIEIAKKLSRSNSTLLLTGESGTGKELFAHSIHNASKQQYGPFVAA
                     NFAALTESLLESELFGYEEGAFTGARKGGHAGLFEQAHKGTIFLDEIGDASPRIQARL
                     LRVLQEKEVLRVGGTKIIPIDVRVIASTNKDLHKLVQKGDFREDLYYRLNVLPINIPP
                     LRERKSDIPLLIKMFLKKFPNGSEMVISNEVMAKFLEYDWPGNVRELENVIEYLYNLA
                     GSKIELKDLPLDLAGGKKQEDIQTQNQQILEKTLKDLEMEIPLQDVLTILNAIKRSKD
                     EGYAYIGRKKLAELVKDEFTEQMIRSRLEILSQAGLIITERGPKGSRLSLKGEKFLDL
                     INNTLE"
     gene            complement(2417..3043)
                     /locus_tag="PLD67_03580"
     CDS             complement(2417..3043)
                     /locus_tag="PLD67_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21633.1"
                     /translation="MNEINDLRLRDWTRDFLVQRIFGRGRNLMTAENAIIENINTADR
                     TVYVTISYGVLGDFNVINMELVRLVVDRDTVILDQFGRPMSSRELREGMRVDAVFSAA
                     MTRSIPPQARAYSIIVIDDGMQSSFTEDTVIEVDLANRFLYTGIPFDIMSQMRYVVTD
                     STTIRDRRGRRIRLRDLRPGDFVRIEHANFMTMSIPPQTTAYDIQVIR"
     gene            complement(3121..3279)
                     /locus_tag="PLD67_03585"
     CDS             complement(3121..3279)
                     /locus_tag="PLD67_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOT21634.1"
                     /translation="MNFADGVILFIIAAIVAAVIKVIKKQNKNNGGCSGCSGCSKANS
                     CPSAKKLS"
     gene            complement(3282..>4863)
                     /gene="feoB"
                     /locus_tag="PLD67_03590"
     CDS             complement(3282..>4863)
                     /gene="feoB"
                     /locus_tag="PLD67_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009271004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOT21635.1"
                     /translation="ADGEKIDQLANVIRSIKEKPIPKRHDFCVGEVHKAIHSISHLIA
                     DQAEKAGYPPRFAATKLVEGDEPTFKSLNITENQMHIIEHVVKDMEEALKTDREAALA
                     DMRYCYIEKICSESVIKKQETKEQQRSEKIDEILTHKYFGIPIFFGIMLFIFWMTFNV
                     IGAPLQEFLEVVIALLTETISNALINIGVSDWVYSLVINGIFVGVGSVLSFLPIIVVL
                     FFFLSLLEDSGYMARVAFLMDKLLRKIGLSGRSFVPMLIGFGCSVPAIMATRTLASER
                     DRKMTIVLTPFMSCSAKLPIYGMITAAFFPDNAALVMIGLYVMGIAVAVLSGLLLKNT
                     IFKGEPVPFVMELPAYRIPSRKSVLIHMWEKAKDFIQRAFTIIFLASIVIWFLQGFNW
                     SFEMVEDSSKSILAYLGSMISPIFRPLGFNDWRASTALITGLTAKEAVVSTLSVLTGA
                     ATDAQLLEALFIIFTPISAFAFLTFVLLYMPCVAAFAATKRELGSLKYAVLTALYQTV
                     TAYTAGAIVYQIGSFIGG"
BASE COUNT         1636 a          903 c          842 g         1482 t
ORIGIN      
        1 gattcattgg aagatttgat taaaaaggca atggctaacg gtgtaaaaat gattgcctgc
       61 accatgtcaa tggatgtaat gggcattaca aaagaagaat taatagatgg tgttgaattt
      121 gcaggagtag gcacttattt gggtgatgcc gaagaatcca atgtaaacct gtttatctga
      181 tactcatgta atgacgactt aaatgttgaa gaagatatac agttatgata taatttattt
      241 aatgactcat tgatgaggag gttcatggtg tcatcttctt taaacaaagg attatctatt
      301 ataacttatt cgaaaaaaca catggagaat gtaattgccc aattaactaa tcttcttgga
      361 gatgaaatca gcatcaaagg atattccata caagaaggaa tcaatgaaga aatttgcgat
      421 tccctggttc tgatatgtca taagtatctg aaggaagaag cgttaaagca cattactccc
      481 ggtgcaaatg tattggtagc cagaagatca cttaatcatt ctaagttaga ggatatttta
      541 caaatacctg aaggggaaaa agtgcttttt gttgacgtca acaaatattt ggcggaaagc
      601 tccatatctc tgcttgtaga actgggtatt aaccatatat cgatgtatcc ctattatccg
      661 ggtcaagaat ttccggaata tgccgactat tctataacaa ccggtgaaac tcacttagtg
      721 cccgattatg tcaaaaatat aattgatatc ggttatagca aaatagacct taccacaatc
      781 acagaaataa tgattaagct cggaattatg gatgaaagag caaatctatt gtcttcaaga
      841 tatttgcagg actttgcgta ttccagcaaa aaaatcgtag aaacactcag tgataataaa
      901 catattacgg agcagttaaa cacgatatta aatgtaataa atgacggtat agtaggtatt
      961 gacggtagtg gtgaaataat gttcagcaat aataatgcaa acaaactctt caacaatacc
     1021 gaaattaatc taaacgatat aaataacttt gatattaagt catttcaagg taagactttt
     1081 gatgtgtacc gtgaaattca tgagataaaa ggtaaaaaag ttcttctcac aatgatgccg
     1141 attattgaag ataatgctaa taatggtata ataatcactt ttaaggactt gtctgaaatt
     1201 atggagatgg aaaatatagt cagagtcaac ctcttcaaaa aaggacatac tgcaaaatac
     1261 cgttttagcg atattgtggg caagagtaaa aatatgagcg agacaataga aattgctaaa
     1321 aagctttcaa gaagcaattc aaccctcctc ttgaccggag aaagcggtac aggcaaggaa
     1381 ttatttgccc attcaattca taatgcttct aaacagcagt atggtccttt tgttgcagct
     1441 aactttgctg cacttaccga aagcttgctt gaaagtgaac ttttcggtta tgaagaagga
     1501 gcctttaccg gtgcaagaaa gggaggccat gcaggacttt ttgaacaggc acataaagga
     1561 acgatatttc tggatgaaat cggtgatgcc tcacccagaa tacaagcacg attgcttaga
     1621 gtacttcagg aaaaagaggt tcttcgtgtc ggaggaacca agattattcc aatagatgtg
     1681 agggttatcg cttcaactaa taaagacctt cataaacttg tacaaaaagg tgatttcaga
     1741 gaagacttat attatcggct taatgttctt ccaataaata ttcctccttt aagagagaga
     1801 aaaagcgata ttcccttgct gataaaaatg tttcttaaaa agtttccaaa tggaagtgag
     1861 atggtaataa gcaatgaggt tatggcgaaa tttttggaat atgactggcc gggtaatgtt
     1921 cgtgaacttg aaaatgtaat tgaatatctt tacaatctgg cgggaagcaa aattgaactt
     1981 aaagatttac ctttggattt agcaggcggg aaaaagcaag aagatattca aactcaaaat
     2041 caacaaatac ttgaaaaaac tcttaaagat ttagagatgg agataccgct tcaagatgtc
     2101 cttaccatac ttaatgctat aaaaaggtca aaagacgagg gatatgctta tataggacgt
     2161 aagaaactgg cagagctagt gaaagatgaa tttactgagc agatgattag aagccgtctt
     2221 gaaatactat cacaggcagg attaataatt accgaaagag gacctaaagg ttcaagatta
     2281 tcgttaaaag gagagaaatt cttagatttg ataaacaaca cgttagaata atccggaatt
     2341 tttgaaaaag ggagcagaac ttctgctccc tttaatctta taattgttag ttttcttcat
     2401 cacttatgat agttatttat ctaataactt gtatatcgta cgcggttgtt tgaggcggta
     2461 tgctcattgt cataaaattt gcatgttcaa ttcttacaaa atcgcccggt cttaaatctc
     2521 ttaatcttat tcttcttcct ctcctatctc tgatggttgt tgaatccgtt actacatacc
     2581 tcatttggct cattatatca aatggaatcc ctgtatacaa aaatctattt gctaaatcaa
     2641 cttctatgac tgtatcttca gtaaaacttg attgcatacc atcatcaata acaattatgc
     2701 tgtatgctct cgcctgagga ggtatacttc tagtcatagc agccgaaaat acagcatcca
     2761 ccctcattcc ttctcttaat tctctgcttg acataggacg accaaattga tccaatataa
     2821 cagtatctct gtcaacaacc aatctgacta actccatgtt tattacatta aaatctccca
     2881 atacaccata tgatatcgtt acatagactg ttctgtcagc tgtatttata ttttcaataa
     2941 tagcgttttc tgctgtcatt aaatttctgc ctcttccgaa aattctttgt actaaaaaat
     3001 ctctggtcca atctcttaat cttagatcat ttatttcatt catattttca cctcgcatat
     3061 atatattatt atatatattc agaagatata aatatgttat tgataaaaaa actgtctcaa
     3121 tcaggacagt ttttttgcag aaggacagct gtttgctttg ctgcatccgg agcagcccga
     3181 gcagccgcca ttatttttat tttgtttttt tataactttg attactgctg caacaattgc
     3241 tgcaataata aaaagaatta ctccatcggc aaaattcatt atcagcctcc tataaagctt
     3301 cctatttgat aaacaatagc tcctgctgta tatgcagtaa ccgtctgata taaggcagtc
     3361 aatacagcat actttaacga gcccaactcc ctttttgttg ccgcaaaagc agcaacacag
     3421 ggcatgtaca ataaaacaaa tgttaaaaat gcaaatgcgc taataggcgt aaatattata
     3481 aacaaagctt ctaaaagctg tgcgtctgtt gcggctcccg tcaatacgct caaagtactt
     3541 acaacagctt ccttggctgt taatcctgta ataagtgccg ttgaagctct ccagtcatta
     3601 aatcccaatg gtctaaaaat cggtgaaatc atggagccta aataagcaag tatgcttttt
     3661 gagctgtctt caaccatttc aaaggaccag ttaaatcctt gcaaaaacca tatcactatg
     3721 gaagccaaga atattattgt aaatgctctt tgaataaagt ccttagcctt ttcccacata
     3781 tgaatcagaa cactttttct tgaaggtatt ctatatgcag gcagttccat tacaaatggc
     3841 accggctctc ccttaaatat agtatttttc aataacaaac ccgataatac ggcaactgca
     3901 atgcccatta catacaagcc aatcataacc aatgcagcat tatcaggaaa gaatgctgca
     3961 gtaatcattc cataaatagg tagcttggca ctgcaagaca taaaaggcgt taaaacaatt
     4021 gtcatttttc tgtccctttc gcttgccaag gtccttgttg ccataattgc aggaacactg
     4081 catccaaagc cgataagcat tgggacaaaa gaccttcccg acaacccgat ttttcttaga
     4141 agcttatcca tcaaaaatgc tactcttgcc atatagccgc tgtcttccaa aagggacaaa
     4201 aaaaagaata agactacaat aatgggaagg aaggacaata cacttccaac gccgacaaat
     4261 ataccgttaa ttacaagtga atatacccaa tcgctaaccc ctatattaat caatgcattt
     4321 gatattgttt cagttaataa agcaattaca acttccaaaa attcctgaag aggagcccct
     4381 ataacattaa atgtcatcca aaaaataaaa agcattatcc caaagaatat aggtatacca
     4441 aaatatttat gtgtcaatat ttcatcgatt ttttcggagc gctgctgctc ttttgtttcc
     4501 tgctttttaa taactgactc cgagcaaatc ttttctatat agcaatatct catgtctgct
     4561 aaggctgctt ctctgtcggt ttttaaagct tcctccatat ctttaaccac atgctctata
     4621 atatgcattt gattttcagt aatattcaag ctcttaaaag taggctcatc tccttcaaca
     4681 agcttagttg cggcaaacct cggaggataa cctgcttttt ctgcctggtc agctattaag
     4741 tgtgaaatgg aatgtattgc tttatgcact tcacctacac aaaaatcatg ccttttagga
     4801 attggtttct ccttaattga ccttatgaca tttgccagct ggtcgatctt ttcaccgtcg
     4861 gcc
//
LOCUS       DAOSOW010000096         4830 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_17647_length_4830_cov_4.105131, whole genome shotgun sequence.
ACCESSION   DAOSOW010000096 DAOSOW010000000
VERSION     DAOSOW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4830)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17647_length_4830_cov_4.105131"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..729)
                     /locus_tag="PLD67_03595"
     CDS             complement(91..729)
                     /locus_tag="PLD67_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21636.1"
                     /translation="MLQWYERDGYYKKRLLREIKFIQEYRSKVPNSFIEYSLLGKHFV
                     VEYLFEYKGKRFIIRCIYPFLYPKVRIKVVVFEIKSKEKKVELFDEGYHNSNGVLCLL
                     LHHPNQWQEEYGIEYIIKRVKEWFENGQYDKNNIVPIDYNRDNELFIFPEPLTGDVDK
                     DYGIFEYCNIREKICVATAIFTRKQKIYVDNLPKSISVDNAKKVRISATSGH"
     gene            complement(731..1702)
                     /locus_tag="PLD67_03600"
     CDS             complement(731..1702)
                     /locus_tag="PLD67_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT21637.1"
                     /translation="MKIHFLNVGHGDTTLVDFDGVYMLIDCNISSTNDAAYKYIDKTI
                     PKPQDKNAKKKLDYLVITHPDKDHITGLDIIDENFDIGEIWESGFRRSDDAEECAEYD
                     TFLELVDKITTKQLSAGSNKLSFSVSNVEAYCLCSKSNDNDDVHYNSLVVKFVEGSKS
                     IIFAGDSNCEAWKNKVVKYYKSLLDADILHASHHGSRTFFFEEGQDKDKDEPYTDGLE
                     AISPDYTVISGCDCDDKVKEDWPPHDDAVALYEEYTSSDGGVYITGKDGNLVFELTDD
                     EIDFNESASRNYDVKKGKFQKYNKLKAPFIAGTGSPQGRLTKDSFGE"
     gene            complement(1722..2444)
                     /locus_tag="PLD67_03605"
     CDS             complement(1722..2444)
                     /locus_tag="PLD67_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21638.1"
                     /translation="MKSLFDKLGIYDFLGFIIPGLLGILSAQVAYKDIFHLPFPLKLD
                     DGFINSFIFIAISYFIGIIMHEISEFVQEKLLKRIWGGFPSERFLLEDDKTCPEIEKE
                     SYYALAKEAFQIEEGKYDKEKSQLVFDRFRTALQLAGKDERSQLFNTYYGMYRNFSTG
                     SLICFLTYFIYSIVFVFNLVVYKVKITNDYSFIVTDIVFLIFTLILFRRTRRFGETYV
                     KYVFRGFAEHYRSAQINQNIKN"
     gene            complement(2552..3379)
                     /locus_tag="PLD67_03610"
     CDS             complement(2552..3379)
                     /locus_tag="PLD67_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21639.1"
                     /translation="MMEIHFIDVGCGDMTLILMPNGTTFVIDCNITNDNKDSVFKYLK
                     KAMGGRSEINVFINTHRDADHMRGIKLLHELYPIAIIWDSGVPGTTTDSSEYKEYMDL
                     RRKIGYKEISARTFWKYGDVKIRVFNSRWKDYSDANSQSIVIKAEYKTKSCSAFITGD
                     TDYRPWKEKILTFYSTDDLKCAIFQAPHHGSITFFDDPSDIRNYYTEHIKKIKPDMTI
                     ISVGSNPHGLPDNKAVELYEKYSKGSSKGNKVYTTKNEGNIKVVFKDDGGWSLSKNL"
     gene            complement(3527..3706)
                     /locus_tag="PLD67_03615"
     CDS             complement(3527..3706)
                     /locus_tag="PLD67_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT21640.1"
                     /translation="MDYITAKEAAEKWGISQRRVQLLCEQGRVIGAVRLGWAWAIPKE
                     ADKPVDARTKAKDKL"
     gene            complement(3866..>4830)
                     /locus_tag="PLD67_03620"
     CDS             complement(3866..>4830)
                     /locus_tag="PLD67_03620"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOT21641.1"
                     /translation="KYLEENGIKTVTGKNQWSTTTINNMLSNEKYMGDALLQKSYTVD
                     YLTKKRVKNNGIVPQYYVEDSHPAIISKDLFHRIQEEKARRANIKKSAEKRTKTDSGK
                     YSSIYALTELLICGECGKPYRRVSWTAYSKKRIVWRCLNRLEYGKKHCQKSPTIDEDV
                     LHRAIMDAFNSLIQDKGDFVDTLQSNIQLVMGNRAKQMDIAKIEERIAELKKEMIGFV
                     EENARCGADNTDFDEHYAKISAELKELHEIKTQYTEQQARQDSFQRRIEDMKKFLNTA
                     DCKLSGFDNQLVRQLIQSIKVMSKDKILIKFKSGLEMEQELATK"
BASE COUNT         1417 a          993 c          696 g         1724 t
ORIGIN      
        1 agcacttaat ttgttagctc gacaaggtgt ccggatgttt ccgatacggt gtccgaataa
       61 ttccgatatg ctgtccggat aattccggta tcagtgtccg gatgtcgccg aaatacgcac
      121 cttttttgca ttatctacac taatactttt tggaagattg tctacatata tcttttgttt
      181 ccttgtaaag atagctgtgg ctacacatat tttttcacga atgttacaat attcaaaaat
      241 cccataatct ttgtctacat ctcctgttaa tggttcaggg aaaataaaaa gttcattgtc
      301 tctgttgtaa tctattggaa caatgttatt tttatcatat tggccgtttt caaaccattc
      361 ttttaccctt tttatgatat actcaatccc atactcttct tgccactggt taggatgatg
      421 taaaagaaga caaaggacac cgtttgaatt atggtatcct tcatcaaaaa gttcaacttt
      481 cttttcttta ctttttattt caaataccac gaccttaatt ctaactttag gataaagaaa
      541 tggataaata caccttatga tgaatctttt gcccttatat tcaaataagt attctacaac
      601 aaagtgtttt cccaataagg aatattcaat aaagctatta ggaactttac ttcgatattc
      661 ttgaataaat tttatttcgc ggagcaatct cttcttataa tatccatctc tttcatacca
      721 ctgcagcata tcactcaccg aaagaatcct ttgtcagtct accttgagga ctacctgttc
      781 cagcaataaa aggagccttc agtttattgt atttttggaa ttttcctttc ttaacatcat
      841 aatttctgga ggctgattca ttgaagtcaa tttcatcatc tgttaactcg aatacaaggt
      901 ttccatcttt acctgtaata taaacgcctc cgtcactgct ggtatattct tcatataatg
      961 caacagcatc atcatggggc ggccaatcct cttttacttt atcgtcacag tcacatccag
     1021 aaatgactgt ataatctggt gagatagctt ctaatccatc tgtatatggt tcatccttat
     1081 ccttgtcttg tccttcctca aaaaagaaag tccttgagcc atggtgactt gcatgaagta
     1141 tatcagcatc aagcaaagat ttataatact tgaccacttt atttttccat gcttcacaat
     1201 tggaatcacc agcaaatata atagatttgc ttccttcgac aaacttaacc accaaagaat
     1261 tataatgtac atcatcattg tcattgcttt tcgaacataa acagtatgcc tcgacattac
     1321 taacagagaa agacagctta ttactgcctg cacttaattg ctttgttgtt attttatcca
     1381 ccaattctaa aaaggtgtca tattcggcac actcttccgc atcatccgat cttctaaaac
     1441 cactttccca tatttctcca atgtcaaagt tttcgtcaat aatgtccagt ccagttatat
     1501 ggtctttgtc tgggtgggta atgacgagat aatccagctt tttctttgca ttcttatctt
     1561 gaggtttggg aatagttttg tctatatatt tatatgcagc atcgtttgta cttgaaatgt
     1621 tacaatcaat taacatatag accccatcaa agtccacaag agttgtatct ccatgtccca
     1681 catttaagaa atgaattttc ataggtatac ctcctcacaa actaattttt tatattttga
     1741 tttatttgag cacttctata atgttctgca aaacctctaa aaacatattt tacataggtt
     1801 tctccaaaac gccttgtcct tctaaaaagt ataagtgtaa atataaggaa aacaatgtct
     1861 gtcacaataa aagaatagtc gttagtaatt tttactttat aaactaccag attgaacaca
     1921 aaaacaatag agtatataaa atatgttaaa aaacatatta aagaacctgt agaaaagttt
     1981 ctatacattc cgtaatatgt gttaaataat tgagaccttt catctttgcc tgctaattga
     2041 agagcagtac gaaatctatc aaatactaac tggctttttt ctttatcata tttaccttct
     2101 tcaatttgaa aggcttcttt tgctaaagca taataggatt ccttctcaat ttccgggcaa
     2161 gtcttatcat cttcaagcaa aaatctttca gatggaaatc ctccccaaat ccttttaagt
     2221 aatttttctt ggacaaattc gctaatttcg tgcattatta tgcctataaa atagctaatt
     2281 gctataaata taaaagaatt tataaatcca tcatcaagtt tcaggggaaa aggcaaatga
     2341 aatatatctt tatatgcgac ttgggctgat aatattccta aaagccctgg aataataaaa
     2401 cccagaaaat catatatacc caatttatca aataatgact tcataattta aacctcaatt
     2461 tcctcttatt gttttaagta cccacaaatc tttttaaagc agtaccttat atttcattaa
     2521 aaaccaattt aaatcttttc cttaattcag cttataaatt tttagacagt gaccagccac
     2581 catcgtcttt aaaaacaact ttaatattac cttcattttt tgttgtgtat actttgttac
     2641 ctttacttga acctttagaa tatttctcat atagttcaac agccttatta tctggtaaac
     2701 catgtggatt tgatccaacg gaaataatag tcatatcagg tttaattttt tttatatgtt
     2761 cagtataata atttctaata tctgaaggat catcaaaaaa agttattgaa ccatggtgtg
     2821 gggcctgaaa tatagcacat tttaaatcat cggtagaata aaaagttaga atcttctctt
     2881 tccaagggcg ataatctgta tctcctgtta taaaagctga acaactcttt gtcttatatt
     2941 ctgctttaat aacaatactt tgtgaattag catcagaata atctttccat ctcgaattga
     3001 atactcttat ctttacatct ccatatttcc aaaacgtcct tgcactaatt tctttatatc
     3061 ctatttttcg ccttaaatcc atatactctt tatattcact tgaatcagta gtagtcccag
     3121 gtacgccaga gtcccaaata attgcaattg gatataattc atgtaataat ttaatcccac
     3181 gcatatggtc agcatctctg tgcgtattta taaaaacatt tatttcagac cttccaccca
     3241 tggctttttt taaatattta aaaactgaat ctttattatc atttgtaata ttacagtcaa
     3301 taacaaatgt tgttccgttt ggcattaaga ttaatgtcat atcgccacag cctacatcaa
     3361 taaaatggat ttccatcaat taattctcct cttaaaatat tcattttgtt attaatgaac
     3421 acattaatag taacatgttc gtgcaactca gatattcctt caggttcgca ttatagacat
     3481 aattccatct acaatcacgt cataaccaac acaaaaaacc aaaagattac aacttgtctt
     3541 tagcttttgt acgggcatct acaggcttgt ccgcttcttt tggaattgcc catgcccatc
     3601 ctagccgaac cgcccctata acccgtcctt gttcacatag aagctgaact ctgcgctggg
     3661 atattcccca tttttctgct gcttcttttg ccgtaatata atccattcct tatacctcgc
     3721 agaaatattt tccatataaa tattgtatac gaagaaacga atgaaaacaa tgaattttgt
     3781 gaatatattt ctgtgatgca ctattcaagc aagtacaata catgaataaa aacagctgcc
     3841 atcctgcttt ttcacagttt gaatgctatt ttgtagcaag ctcctgctcc atctccaaac
     3901 ctgacttgaa ttttatgaga atcttatcct ttgacatgac cttgatgctt tgtataagct
     3961 gcctgacaag ctggttgtca aatccagata atttgcagtc tgcggtgttc agaaacttct
     4021 tcatatcctc gattcttcgc tggaagctgt cttgcctggc ttgctgctcg gtatactgcg
     4081 tttttatctc atgaagctct ttcagctcgg cagagatttt ggcatagtgc tcgtcaaagt
     4141 ccgtattatc tgctccacat cttgcatttt cctctacgaa gcctatcatc tctttcttta
     4201 attccgcgat cctctcctca atttttgcta tatccatctg cttggctcga ttacccatca
     4261 ccagctgaat attggactgg agcgtgtcca caaaatcccc cttatcctgt atgagggaat
     4321 tgaatgcatc cataatggct ctatgcaaaa catcttcatc aatggtcgga gacttttggc
     4381 aatgtttttt tccatactcc agcctgttta agcaccgcca gacaattctt ttcttgctgt
     4441 atgctgtcca ggatacccgt ctgtaaggct tgccgcactc cccgcatatc aataattcgg
     4501 tgagagcata tattgagctg tatttcccgc tgtcggtttt ggttcttttt tcagcactct
     4561 ttttaatgtt agcccggcgt gctttttctt cctgtatccg gtgaaacaag tctttggaaa
     4621 tgatggccgg atggctgtcc tccacataat actgcgggac aattccatta tttttaactc
     4681 ttttctttgt gaggtagtct acggtgtagc ttttttgcag cagggcatct cccatgtact
     4741 tctcattgga gagcatgttg ttgatggtag ttgtagacca ttggtttttt ccggtaacgg
     4801 ttttaatgcc gttttcctct aagtactttt
//
LOCUS       DAOSOW010000097         4802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_17762_length_4802_cov_4.373920, whole genome shotgun sequence.
ACCESSION   DAOSOW010000097 DAOSOW010000000
VERSION     DAOSOW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17762_length_4802_cov_4.373920"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PLD67_03625"
     CDS             <1..508
                     /locus_tag="PLD67_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOT21642.1"
                     /translation="KENINVYSMHTSYDMADKGVTYSLAKRLNILNYQVLHPINIDNS
                     GYGGIGDVAPVNIIEYTKLVKEALGAEYVKLYCNGEKTVRRVAFCGGSGGEFIEDAIR
                     QKADLYITGDIKYHQAQEAVKNNLCIIDAGHYNTEIYSMETLMEVLEKTGLEIINFKK
                     NITAEKII"
     gene            517..2442
                     /locus_tag="PLD67_03630"
     CDS             517..2442
                     /locus_tag="PLD67_03630"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOT21643.1"
                     /translation="MDKNFEYIRVASAVPSIRVADPNYNAIKIKEIILKAEKEKVRVL
                     VFPELCLTGYTCQDLFSQNTLINKCEEALSLLLEETKYLNMLIAVGMPVKADTKLFNC
                     AVIFKEGKILGVVPKTYIPNYKEFYEMRWFVPASYRLSDIVTLCEQEVIFSENLLFKD
                     DISSLCVGVDICEDLWATIPPSSYHALQGANLILNLSASSETSGKADYRRDLVKMQSS
                     KCITSYVYTSSGEEESTTDVVFSGHCIIAEDGKILKEDNLCDGFIYSDIDIEKINNEK
                     RSLNVYNSNIERKNYKYVNFNLGYSENLKLERFVEPRPFIPARPEDRDKSFSNILKIQ
                     AAGLSQRMKKIGSKKAILGISGGLDSTLALIVAVEAIKKLKLPLTNIITVIMPGFGTS
                     ERTYKNAHILIDELGVTLREISIKDACMQHYKDIGHDINNHDVTYENSQARERTQILM
                     DIANQEGGLVVGTGDLSELALGWCTYNGDHMSMYSVNSGVPKTLKNMVKWYADSKTSG
                     EMQKTLLDICNTPVSPELLPPDKEGNIRQLTENSVGSYELNDFFLYNMMKNGYGPEKM
                     YYLAKIAFKEEYDEQVILETLKNFYKRFFTQQFKRSCMPDGVKVGSVSLSPRGDWKMP
                     SDASYELWMKICENIKK"
     gene            2498..2915
                     /gene="rnpB"
                     /locus_tag="PLD67_03635"
     ncRNA           2498..2915
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLD67_03635"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(3247..4470)
                     /locus_tag="PLD67_03640"
     CDS             complement(3247..4470)
                     /locus_tag="PLD67_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT21644.1"
                     /translation="MKLHIYSLIMFLNSYLTGLLTPVLSLALIDKGASLSNLSIILGL
                     YALSVILLELPSGIMADVFGRKKTFVLSLVLFTLSFSLLLISKVFIVLCIVMILYGLG
                     RALGSGSFDALFIDYYIDNFGKDKLHNITTRLSVLDALGLSAGALSGGFFPDISNNLL
                     PSIGTYDLNIIVRIVLTIAVIIISVLFISEKNHHDKKEHKSIKQHVKNSSDFVRKNTT
                     ILFIFISVFSTGIFLSALETYWQPHFITLLPDEGSSGLLGLMAFLYLGAALVGSILSN
                     KIMSKYKFNIRKMYLIMRSLLALFLILTALQTNIPLFIAYYASIYLMFGMANIPEDVI
                     LNSETPNQIRASVLSVKSFTIQIGGLSGSLMYSILIRFISIPAIWVIAASIILLTIAL
                     IAKKFIAAEKARNKI"
BASE COUNT         1752 a          729 c          950 g         1371 t
ORIGIN      
        1 taaagaaaat ataaatgttt acagcatgca cacaagttat gacatggctg ataagggagt
       61 tacatatagc ttggcaaaaa gactgaatat attaaattat caggttcttc atccaataaa
      121 tattgataac agcggatatg ggggaatagg agatgtagct cccgttaata taattgagta
      181 taccaaatta gtaaaagaag ctcttggagc tgaatatgtc aaattgtatt gcaatggaga
      241 aaaaacagtt agaagggttg cattttgcgg cggcagcggc ggtgaattca ttgaggatgc
      301 tataaggcaa aaagctgatc tttacataac cggagatata aaatatcacc aggcacagga
      361 agctgtaaaa aataatttat gcattattga tgcaggtcat tacaacacgg aaatatactc
      421 aatggaaaca ctaatggaag tattagaaaa aacaggattg gaaataatta attttaagaa
      481 aaatataaca gctgaaaaaa taatttagag gtggatatgg ataagaattt tgaatatata
      541 agagtcgctt cggcagtacc atccataaga gttgcagacc caaattataa tgcaattaaa
      601 attaaggaaa taatattaaa agcggaaaaa gaaaaagtca gagtcttggt atttcctgaa
      661 ctttgtttaa cgggatacac ttgtcaagat ttgtttagcc agaatacatt gataaataaa
      721 tgcgaagaag cattaagcct tttattggaa gaaacaaagt atttgaatat gcttatagct
      781 gtgggaatgc ccgtaaaagc cgacaccaag ctgtttaatt gcgccgtaat atttaaggaa
      841 ggaaaaatac ttggtgttgt tcctaaaaca tatataccta attacaaaga gttttatgaa
      901 atgagatggt ttgttcctgc atcttacagg ttatcagaca tagttacact ttgcgagcag
      961 gaagtaattt ttagcgaaaa ccttttgttt aaggatgata tatcttcgct atgtgttgga
     1021 gtggatatat gtgaagattt atgggcaact attcctccaa gctcatatca tgctttgcag
     1081 ggtgccaact taatacttaa tttgtccgcg agcagcgaaa catcaggtaa ggcagattac
     1141 cgaagagatt tggtaaagat gcaatcttcc aaatgcataa catcatatgt ttatacttct
     1201 tccggcgaag aagaaagcac aactgatgta gtattttccg gacactgcat aattgcggaa
     1261 gacggcaaaa tattaaagga agataattta tgcgacggtt tcatatattc agatattgat
     1321 atagaaaaaa taaataatga aaaaagaagc ttaaatgttt ataattcaaa tatagaaaga
     1381 aaaaattaca aatatgtcaa ttttaattta ggctacagtg aaaatttgaa gcttgaaagg
     1441 tttgttgagc ccagaccttt tatacctgca agacccgaag acagagataa aagtttttct
     1501 aatatcctaa aaattcaggc tgcaggtctt tctcagcgca tgaaaaagat tggttcaaaa
     1561 aaagccattc tggggatatc aggaggtcta gacagcacat tagcattgat tgtggcggtt
     1621 gaagcaatta agaaattaaa attaccgctt acaaatataa ttactgttat tatgccggga
     1681 tttggaacat cagagagaac ttacaaaaat gcccatatac tgattgatga attaggagta
     1741 accttacgag aaatatccat aaaggatgca tgcatgcaac attataaaga tataggacat
     1801 gatataaaca atcatgatgt tacttatgaa aattcacaag caagggaaag aacacaaatt
     1861 ttaatggata ttgccaatca agaaggagga ctcgtggtag gaacaggtga tttatccgag
     1921 cttgccttag gatggtgcac atacaacggg gaccatatgt caatgtacag cgttaattca
     1981 ggagttccca agaccttaaa aaatatggtt aagtggtatg cggattctaa aacatcagga
     2041 gaaatgcaaa aaacacttct tgatatatgc aacactcccg taagccctga actgcttcca
     2101 ccggacaagg aaggaaatat tcgtcagctt acggaaaact cggtaggctc atatgagctg
     2161 aacgactttt tcctgtacaa tatgatgaaa aacggttacg gaccggaaaa aatgtactat
     2221 ttggcaaaaa tagcttttaa agaagaatac gacgaacaag ttatccttga aacattaaaa
     2281 aatttctata aaagattttt tactcagcaa tttaagcgct cctgcatgcc tgatggggtt
     2341 aaggtaggct ccgtatcatt gtcgcccaga ggagattgga aaatgccaag cgacgcatct
     2401 tatgaattat ggatgaaaat atgcgaaaat atcaaaaaat aaaattcttg gataaatctg
     2461 catttgacat aaaattataa atattataaa ataacaaaga gtaaattata caatcgcatg
     2521 catagcatga ggaaagtccg agctccatag agcagggtgc cgggtaaccc ccggtgaggg
     2581 tgaccttaag gatagtgcaa cagaaagaaa ccgccccgca ctcaatctgt aagagattca
     2641 gtttattatt taagatatgt aaactataaa ttaatttagt atagagtctt ttacagattg
     2701 agtgcggggt aaggatggaa aggtgaggta agagctcacc ggcaatcagg cgactgattg
     2761 gccatgtaaa ccccacccgg agcaagacca aaagaggacc ttggagtggc tgctcgtcac
     2821 ggtccaaatg gtaggtcgct tgagtttgcc ggcaacggca agcctagata gatgattgtt
     2881 taatacagaa ctcggcttat atatttactc actacatata taatagaaaa taaaatcatt
     2941 agcctttgct aagacgaagg tttttttgtt ataatttgta acaatatcat cttcattgaa
     3001 taaaataagt acaggcaata taaaaaggga tgatgatatg ttttgtagag tagtgccatg
     3061 gtttttactt ggtgttggtt tcggaagatg cagcgagaga cgtgatgtct taggagctag
     3121 gggaagattt gacccatgca tcagaagaag aagataataa aataagggac ataacttcgg
     3181 tattatctgc agtgggcagc cgcaaggtgt gtcccattct cttatatata gattcaggct
     3241 gtatatttag attttattgc gggctttttc ggctgcaata aatttttttg caattaatgc
     3301 aattgttaat aaaattatgg aagctgcaat tacccaaatt gcgggtattg atataaatct
     3361 tattaaaata ctgtacatta atgagccgga taagcctccg atttgtattg taaatgattt
     3421 taccgacagc acggaagccc ttatttggtt gggagtttca gaattaagta ttacatcttc
     3481 cggaatatta gccatgccga acattaaata aattgaggca taataggcaa taaacaaggg
     3541 aatatttgtt tgtaatgcgg ttagtatcaa aaatagagca agtaagcttc tcattattag
     3601 atacatcttt cttatgttaa atttatattt gctcataatt ttgtttgaaa gaatacttcc
     3661 taccaaagct gcgcccaaat ataaaaatgc catcaatcct aagagacccg aggagccttc
     3721 atcgggtaaa agtgttataa agtgaggctg ccaataggtt tctaatgcgc ttagaaagat
     3781 acctgttgaa aaaacagaaa tgaatataaa aagtatggtg gtgtttttgc gaacaaaatc
     3841 agaactgttt tttacatgtt gtttaatgga tttatgttct tttttatcat gatggttttt
     3901 ttccgaaata aaaagaacag atataattat gacagcaatt gttaaaacaa ttctaacaat
     3961 aatatttaaa tcataagttc ctatggaagg aagaagatta ttagaaatat ccgggaagaa
     4021 tcctccggat aaagcacccg ctgaaagtcc caatgcatct aagactgaaa gccttgttgt
     4081 aatgttatgt aatttgtcct taccgaaatt atctatatag tagtcaatga atagcgcatc
     4141 aaaagatcct gagccgagag ctcgtcctaa accgtaaaga atcatcacaa tacataatac
     4201 tataaagact ttgctaatca ataataatga aaaacttaaa gtaaataata caagggataa
     4261 tacaaaggtt ttttttctac cgaagacatc tgccattatt ccggaaggca gttcaagcaa
     4321 aataaccgac aaggcatata atcctaatat aattgataag ttactcaagg aggcgccttt
     4381 atcaattaaa gccagactaa gtaccggggt taacagaccg gtcaaatatg aattaagaaa
     4441 cattattaaa gaatagatgt gcagcttcaa atttctacct ccaaaggtaa ctattgcacc
     4501 gtataataat tataatctaa aagagtatta gagtcaagcg caattaaagc atatattgcg
     4561 cgctctgcgg tcaaaaaata atcagctttt attagagaaa tacaaaaaaa gacgcacggg
     4621 tctttgacac ttgaagaata agaaaagcga taaagtcatt ggaaagactt tatttttttg
     4681 tcaaattttg aaaataattg ttgcacaatg tttcacaatg tgttataata taagcaaaaa
     4741 ccgcggaggt tagatatgag gttacagatt tctaaatcca aaaatgcttc atcattttat
     4801 gt
//
LOCUS       DAOSOW010000098         4765 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_17935_length_4765_cov_3.165605, whole genome shotgun sequence.
ACCESSION   DAOSOW010000098 DAOSOW010000000
VERSION     DAOSOW010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17935_length_4765_cov_3.165605"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PLD67_03645"
     CDS             <1..726
                     /locus_tag="PLD67_03645"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF015446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOT21645.1"
                     /translation="ILSDKITAGLNTAAIRLPENKIALALIRESKKPIAAPSANTSGS
                     PSPTEASHVIEDLMGKVDMIIDGGSTYIGLESTVVDMTSSVPMILRPGGVTAEDIISV
                     LGECSYDPAIIKSEEIVIPKSPGQKYRHYSPKAKVILYKGKIERIVEKINEDYDKFTS
                     LGLKTGIMSTAQTEEFYKGKINICIGDRTKLLTISSNLFKGLRDFDHMGVNVILAEAV
                     DEKGLGKAIMNRLGKSASETVEV"
     gene            728..1216
                     /locus_tag="PLD67_03650"
     CDS             728..1216
                     /locus_tag="PLD67_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HOT21646.1"
                     /translation="MNILFVCTGNTCRSPMAEGIFKDMLMKNNINNISVSSAGISVFP
                     GEHASEKAIKALKEKGIDITGHRARQLRDEINNVDLILTMTLSHKMIISDYLKNETDE
                     KSPRLFTLKEFAAKLSGEKLTDTDIVDPYGKNYNFYKNSRDEIEKELIKILKNLDKLE
                     GI"
     gene            1213..1668
                     /gene="rpiB"
                     /locus_tag="PLD67_03655"
     CDS             1213..1668
                     /gene="rpiB"
                     /locus_tag="PLD67_03655"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019595527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOT21647.1"
                     /translation="MKIVLASDHGGFELKEAIKKHLIKKGYDINDIGVYDTKSVDYPD
                     YGKRAALMVADKEADRGIIVCGTGIGISIAANKIKGIRCALCTNEYMARMSRMHNNAN
                     MLALGGRVTGIGLATDMVDVWLSTEFEGGRHENRVNKIMDIEISKEEEK"
     gene            1668..2696
                     /locus_tag="PLD67_03660"
     CDS             1668..2696
                     /locus_tag="PLD67_03660"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOT21648.1"
                     /translation="MTANLIAFISAVVISFVMTPWARRLAIKVGALDIPKEARKVHNK
                     PMPYFGGLAIYVSIIACMFVYMPHTVTNYYIMAGATVIVLAGVVDDMYGMPAKIKLLI
                     QIIAALIAIRGGVQIHFITNPLSETGMSYLFDWLSFLITLFWLVGITNTINLIDGLDG
                     LASGVASIAATTLLFTAARLGHDFIVMQCAIIAGASLGFLPFNFNPAKIFMGDTGSLL
                     LGYMLAVTSVSGMVKSVAVVALAVPVFALGLPIFDTTFAIIRRYINKKPIMEADKEHL
                     HHKLMGVGLNQRQTVLIMYFISMLLGIVSVIIADADPFTGLTVATAVVIIAFIFAKKA
                     GLFVRKEQ"
     gene            2705..3826
                     /gene="wecB"
                     /locus_tag="PLD67_03665"
     CDS             2705..3826
                     /gene="wecB"
                     /locus_tag="PLD67_03665"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOT21649.1"
                     /translation="MDKIKVLVVFGTRPEAVKMAPIVKALKKDEDNFISKICVTAQHR
                     DMLDQVLRIFDIEPDYDLNIFENGQTLTQITCRALTGLEEVIEDFKPEIILVQGDTTT
                     VFAGALAAFYHQVKIGHVEAGLRSGNLYSPYPEEANRMMTGVLTDFHFAPTETSKKNL
                     IREGYDENKIFITGNTSIDALRWVIDDNYKFEEEIINNIDFENKRVILLTAHRRENIG
                     KPMENIFSALKDIAEENQDVEVIYPMHLNPKVREIAHRIFGNLENVHLIEPLDYLPFT
                     NLMNKCYLVVTDSGGVQEEAPSLGKPVLVVRRETERPEGIEAGTAKLAGTDRENIYNE
                     LHLLLNNEEEYKKMANAVNPYGDGKAAEYIIQAIKRNYK"
     gene            4002..4223
                     /locus_tag="PLD67_03670"
     CDS             4002..4223
                     /locus_tag="PLD67_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOT21650.1"
                     /translation="MLEKIKEILVEELDVEEDSITLDSKIKEDLGADSLDLFELINRL
                     EDELDVVIEEDDYGKLITVGDIVNYLTDK"
     gene            4256..>4765
                     /locus_tag="PLD67_03675"
     CDS             4256..>4765
                     /locus_tag="PLD67_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOT21651.1"
                     /translation="MLRRVMIYTKICEMLNIKYPIFQGGMAQISDAGLAAAVSEAGGL
                     GIIASGNNPAEKIKAEIHRIRELTDKPFGVNVMLLSPYAKEVSELLIEEKVPAIITGA
                     GNPGKYMKIWKEAGIKVIPVVPSVAFAKHFEKAGVDAVICEGTEAGGHVGELTTMCIT
                     PQVVDAVNIP"
BASE COUNT         1681 a          686 c         1067 g         1331 t
ORIGIN      
        1 atattatcag ataaaattac tgcaggatta aatacagctg ccatacggct gcctgaaaat
       61 aaaattgcac ttgctttaat aagagaaagc aaaaaaccta ttgcagcgcc cagcgcaaat
      121 acatcgggca gtccaagtcc tacggaagct tctcacgtaa tagaagattt aatgggaaag
      181 gtggacatga taattgatgg aggaagtact tatatcggtc ttgaatcaac tgttgtggat
      241 atgacaagct ccgtgcccat gatattaaga ccgggcggag tgacagcaga ggatataatt
      301 agtgttcttg gtgaatgcag ttatgaccct gcaattataa aaagcgaaga aatagttatt
      361 ccaaaatcac cgggtcagaa atacaggcat tattctccca aggcaaaagt tattttgtat
      421 aagggtaaaa tagaaagaat tgtagaaaaa atcaatgaag attatgataa attcacgtct
      481 ttgggattaa agacggggat tatgtcaact gctcaaacag aagagtttta taaggggaaa
      541 ataaatattt gcattggaga cagaacaaaa ctgcttacaa tttcgtcaaa cttatttaag
      601 ggtttaaggg attttgacca tatgggagta aatgtaatac ttgcggaagc agttgacgaa
      661 aaaggtctcg ggaaggctat aatgaacagg ctcggtaagt cagccagcga aacagtcgag
      721 gtatagaatg aatattttgt ttgtttgtac gggcaataca tgcagaagcc ctatggcaga
      781 aggtattttt aaggatatgc ttatgaagaa taatattaat aatattagcg tatcttctgc
      841 cgggataagt gtttttcccg gagagcatgc cagtgaaaag gcaataaagg ctttaaaaga
      901 aaagggaatt gatataacag ggcacagagc aagacagctg cgtgatgaga ttaataatgt
      961 tgacctgata ttaacaatga ccttatctca taaaatgatt atttctgatt atttaaaaaa
     1021 cgagacagat gaaaagtctc ctcgtctttt taccttgaaa gaatttgctg caaaattaag
     1081 cggtgaaaaa ttgacagata ctgatattgt cgacccttat ggcaaaaatt ataattttta
     1141 caaaaatagc agagatgaaa tcgaaaaaga actaataaag atactcaaaa atttagataa
     1201 gctggaggga atatgaaaat agtattggca agcgaccatg gaggatttga attaaaggaa
     1261 gcaattaaga aacatttaat aaaaaagggc tatgatataa atgatatagg tgtttatgat
     1321 acaaaatccg ttgattaccc tgattacggt aagagggctg ccttgatggt agctgataaa
     1381 gaagccgata gaggtattat tgtttgcgga accggaatag gtatttctat tgcagcaaac
     1441 aaaattaaag gaataagatg tgccttgtgc acaaatgaat atatggccag aatgtcaaga
     1501 atgcacaaca atgcaaacat gcttgcatta ggcggcagag ttaccggtat aggtcttgca
     1561 acggatatgg ttgatgtttg gctttctact gaatttgaag gcggaagaca tgaaaacagg
     1621 gtaaacaaaa ttatggatat agaaatttct aaagaagagg aaaaataatg actgcaaatt
     1681 taatagcttt catatcagct gttgttatat cattcgtaat gactccatgg gcaagaagac
     1741 tagcaataaa ggtgggagca ttggatatac ccaaggaagc aagaaaagtc cacaacaagc
     1801 cgatgcctta ctttggagga cttgcaatat atgtatcaat aattgcatgt atgtttgttt
     1861 atatgcctca cactgtcacc aactattata ttatggcagg ggcgacagta attgtgcttg
     1921 caggtgttgt tgatgatatg tacggcatgc ctgcaaaaat taaactcttg atacagataa
     1981 tagcagcatt aatagcaata agaggcggcg tacagataca ttttataaca aatccgcttt
     2041 cggaaacagg tatgagctac ttgttcgatt ggttatcttt tcttattacc ttattctggt
     2101 tagtgggtat tacaaataca ataaatttaa ttgacgggct tgacgggctt gcttcagggg
     2161 ttgccagcat tgcagccaca actcttttat ttaccgcagc aagattgggg catgacttca
     2221 ttgtgatgca gtgtgcaatt attgcaggtg cttcactggg gtttttgcct tttaatttta
     2281 atccggcaaa aattttcatg ggagatacag gctccttgtt gctggggtat atgcttgcgg
     2341 ttacatctgt ttcgggtatg gtaaagagcg ttgcagttgt tgcattggct gtcccggtat
     2401 ttgcattagg tctgcctata tttgatacta cgtttgcaat tataagaaga tatataaata
     2461 aaaaacctat aatggaagca gacaaggaac atcttcatca taagcttatg ggagtcggcc
     2521 ttaatcagcg gcagacagtc ttaataatgt attttataag tatgcttttg ggaatagttt
     2581 cggtaataat agcagatgca gaccccttca ccggtttgac ggttgctact gcagttgtta
     2641 taattgcatt tatatttgca aaaaaagcag gactgtttgt aaggaaagag caataggagg
     2701 aaacatggat aaaattaagg ttttggtagt gttcggaacc cgtccggagg ctgtaaaaat
     2761 ggctccaatt gtgaaggctc ttaaaaagga tgaggataat ttcatatcta aaatttgcgt
     2821 aactgcccag cacagggata tgcttgacca agtactgagg atttttgata ttgagcccga
     2881 ttatgattta aatatttttg aaaacggtca aacacttaca caaattacat gcagagcatt
     2941 aacagggtta gaagaagtaa tagaagattt taaacctgaa ataatactgg tacagggaga
     3001 tacaacaacg gtatttgcag gagctcttgc tgctttttat catcaggtta aaataggtca
     3061 tgtggaagca ggacttagaa gcggcaacct gtattcgcct tatcctgagg aagcaaatag
     3121 aatgatgaca ggtgtattga ccgattttca ttttgctccg acagaaacat ctaagaaaaa
     3181 tctcataaga gaaggctacg acgaaaataa aatatttata accggaaata cttcaataga
     3241 tgctttaaga tgggttatag atgataatta taaatttgag gaagaaataa tcaataatat
     3301 agattttgaa aataaaaggg taatactctt gactgctcac agaagagaaa atatcggcaa
     3361 accgatggaa aatatatttt ctgctctgaa ggatattgca gaagaaaatc aggatgtaga
     3421 ggttatatat ccaatgcact taaatcctaa ggtaagagaa attgcccata gaatatttgg
     3481 caatttggaa aatgttcatt tgattgaacc cttagattat cttcctttta caaatttaat
     3541 gaacaaatgt taccttgttg tcacagattc gggaggggtt caggaagaag ctccatcatt
     3601 gggaaaacct gtcttggttg tgagaagaga aacggaaaga ccggaaggta ttgaagcagg
     3661 aactgcaaaa ttagcaggta cagacagaga aaatatttac aatgaactgc atttattgct
     3721 caataacgaa gaagaatata aaaagatggc taatgccgta aatccatacg gtgacggaaa
     3781 ggctgcagaa tatataattc aagcgataaa gagaaattat aaatagtgac aacatccccg
     3841 tttttttgac actcaaaaaa acagggccat gatgtaggca aaaaatatat tgacaagggt
     3901 gttttgttgt attatacttt gaatatcaaa atacttgaat atgaataaat ctaataatca
     3961 aagtaataga taatattagt atattaggag gaaatattat gatcttagaa aagattaagg
     4021 agattttggt tgaagaatta gatgtggagg aagatagtat taccttagac agtaaaatca
     4081 aggaggactt aggagcagac tcattggacc tatttgaact gataaacaga cttgaggatg
     4141 agctggatgt agtaattgaa gaagacgatt atggtaagct aataacagtg ggtgatatag
     4201 taaattacct tacagataaa taataataaa taaaagagac gccgcaaggc gttttgtgct
     4261 aaggagagtc atgatttata ctaagatatg tgaaatgcta aatataaaat atcccatttt
     4321 ccaaggagga atggcgcaaa tatcggatgc aggattagca gcagccgtat ccgaagcagg
     4381 agggcttggc ataatagcgt ccggtaataa tccggctgaa aaaataaaag ctgaaataca
     4441 tagaatcaga gagttgacgg ataaaccgtt tggagtaaac gtaatgcttt taagccctta
     4501 tgccaaagag gtcagcgaat tattgataga agaaaaagtt cctgcaatta taacgggggc
     4561 gggcaatccc ggaaagtata tgaaaatatg gaaggaagca gggattaagg taatacctgt
     4621 tgttccgtct gtagcttttg ctaagcattt tgaaaaagca ggggttgatg ccgtaatttg
     4681 tgaaggaact gaagcaggag gacatgtggg agaattaacc accatgtgta ttactcctca
     4741 ggttgtggat gctgttaata ttccc
//
LOCUS       DAOSOW010000099         4705 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_18227_length_4705_cov_3.376344, whole genome shotgun sequence.
ACCESSION   DAOSOW010000099 DAOSOW010000000
VERSION     DAOSOW010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4705
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18227_length_4705_cov_3.376344"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="PLD67_03680"
     CDS             <1..282
                     /locus_tag="PLD67_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOT21652.1"
                     /translation="KKLLKAYLSTYDHSDDQSQWFEKIRVIASENGYAAKPKDYKKNP
                     DMYKGHVGDVSTAIRIAIIGRASSPDLWELQQIMGEEKVRRRIENAINS"
     gene            complement(408..2327)
                     /locus_tag="PLD67_03685"
     CDS             complement(408..2327)
                     /locus_tag="PLD67_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOT21653.1"
                     /translation="MGLTAQSVKLESLRDIYEIEKNDKQYVVALAGNPNTGKSTVFNY
                     LTGLNQHTGNWPGKTVVSARGQFKYKNNEYILIDLPGSYSLFTASKDEEVTRDFLCYG
                     NYDTVVVVADATCLERNLNLVFQVSELTNKIILCINLIDEAKKKNIKIDKEGLEKELK
                     IPIVLTSARNGQGMKELKDLIEKTSQIIEEETEKSLYFNQDESIREKISEDNYNYANK
                     LKQKYVKEDVNKLNRDKKIDDIVTSKKFGIPIMLALLGLIFWITIQGANIPSQILADL
                     LFGLQYKLDKILLILGVNDFIRGVFIDGMYKSLAWVISVMLPPMAIFFPLFTLFEDLG
                     YLPRVAFNLDHLFKRAGAHGKMCLSMCMGFGCNAAGVIGCRIIDSPRERLIAVITNNL
                     VPCNGRFPLIIVLSAAFFAFTGSFVDSIIPALSITLIVLIGVFATLSVSYLLSNTLLK
                     GIPSTFTLELPPYRAPQVGRIIYTSLIDRTIFVLSRAAVVAAPAGAIIWLLANIYVGE
                     LSILIHMANFFEPLGRAIGLDGFILIAFIAGFPANEIVIPVLLMAYMSTGTLTDFESI
                     DSLRQILISNGWTQLTAINVMLFSLLHWPCSTTLLTIKKETGSLKWTLAAFIIPTGIA
                     FLVCFITANLYRIIW"
     gene            complement(2339..2557)
                     /locus_tag="PLD67_03690"
     CDS             complement(2339..2557)
                     /locus_tag="PLD67_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOT21654.1"
                     /translation="MKLADVGVGKTVKVKQNSAHDYLKERFLALGLTKGADVEVLRKG
                     PKNNLTVYRIRGAMIALRNEESSLIEVL"
     gene            2682..3113
                     /locus_tag="PLD67_03695"
     CDS             2682..3113
                     /locus_tag="PLD67_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HOT21655.1"
                     /translation="MKEEFYTLKGYKIKNEEILSSSMEDYIEMICRLSEKSDEVRVSE
                     LSSALNVQPPSTTKMIRRLAKENYVTYERYGFIKLTEKGREVGSKLLERHRIIFEFLE
                     CIGVKENILEQTEIIEHAVNDEVLNKIDELTQFLKKCDKYK"
     gene            complement(3222..3350)
                     /locus_tag="PLD67_03700"
     CDS             complement(3222..3350)
                     /locus_tag="PLD67_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HOT21656.1"
                     /translation="MFKKLTNKLKMRYRRSANPGNVNIPGEFEVQERKKDVDNNKK"
     gene            3489..4262
                     /locus_tag="PLD67_03705"
     CDS             3489..4262
                     /locus_tag="PLD67_03705"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21657.1"
                     /translation="MTEVDMVYICELRNVSKRFHTINNETKAIDDISFKVKKGEIVSI
                     VGPSGSGKSTVLSLIAGLEKPSEGEVIVNTDSIGFMFQKDQLYEWRNVYKNCLLGLEV
                     KKMDNEENKIKVKRMLEKYGLTNFKNHYPSQLSGGMRQRAALVRTLAIEPELLLLDEP
                     FSALDYQTRLSIGDEVGTILRNEKISTILVTHDIPEAVSISDRVIVFTERPAKIKKEY
                     EIKFEECPGERTPMKCRNTLAFGEYFRNIWRDLDVGKEV"
     gene            4243..>4705
                     /locus_tag="PLD67_03710"
     CDS             4243..>4705
                     /locus_tag="PLD67_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT21658.1"
                     /translation="MWEKKFEPVANISEEHKEYLKLINREKNFIRIAQILIFVFGIIF
                     WEVAARKNLIDTFLTSSPGAIFGLIMKLVDDGSLFKHLFISTMETIGGFFVGTAGGLL
                     IAIILWWFRKLAKVMDPYIVILNSLPKTALAPIIILWVGMGYSGIIATAASI"
BASE COUNT         1587 a          778 c          861 g         1479 t
ORIGIN      
        1 aaaaaacttc ttaaagctta cttaagtacc tacgaccaca gtgacgacca aagccagtgg
       61 tttgaaaaga taagagtaat tgcatctgaa aacggatatg ctgcaaagcc aaaggattac
      121 aagaaaaacc cggatatgta caaaggacat gtgggagatg taagtaccgc tataagaatt
      181 gcaatcatag gccgcgcatc atcaccggac ttatgggaat tgcagcagat aatgggagaa
      241 gaaaaggtta gaagaagaat tgaaaatgca ataaacagtt aagggacagt ggggacgtac
      301 actactgtcc cgctgttata gtttagggtt tgagttgaaa tactcaaacc cttttgattt
      361 tgttatttga ttgtagtgaa tgggacagtt agtgtacgtc cccagcgtca ccaaattatt
      421 ctgtataaat tggctgttat aaaacatacc aaaaatgcga taccggtagg gattataaat
      481 gctgccaaag tccatttcaa acttccggtt tcttttttta ttgtcaataa ggttgtggag
      541 cagggccagt gaagcaggga aaacaacata acatttatgg cggttaattg agtccaacca
      601 tttgatatca atatttgtct tagagaatca atactttcaa aatctgtcaa agtacctgtc
      661 gacatatatg ccatcaatag aaccggaatt actatttcat ttgcaggaaa accggctata
      721 aaagcaataa gtatgaatcc gtcaagtcct attgctcttc ctaagggttc aaaaaaatta
      781 gccatatgaa tcagaatgct taactcaccc acataaatat ttgctaaaag ccatataatt
      841 gctcctgcag gtgctgcaac aactgcagct ctagacaaca caaaaattgt tctgtcgata
      901 agtgatgtat aaattattct gcctacctgg ggagccctgt agggaggcag ctccagagta
      961 aaggttgagg gaatcccttt taataaagta ttagacagta aatatgacac tgacaaagtt
     1021 gcaaatacac ctattagaac tatcaaggtt attgataaag caggtataat gctgtcaaca
     1081 aatgagccgg taaaagcaaa aaatgctgct gacaatacta taatcagcgg gaatctgccg
     1141 ttgcagggaa caagattatt ggtaatgacg gcaattaatc tttcccttgg cgagtcaata
     1201 attctgcatc ctataactcc tgctgcattg catccgaacc ccatgcacat tgaaaggcac
     1261 attttaccgt gagcgcctgc tcttttaaac agatggtcca aattaaatgc taccctcggc
     1321 aaataaccca agtcttcaaa taaagtgaac aaggggaaaa atattgccat aggaggaagc
     1381 attacagaaa taacccatgc aaggctttta tacataccat cgataaaaac acctcttata
     1441 aaatcattta cacctaagat tagaagaatt ttgtctaatt tatattgtaa accgaacaat
     1501 aaatcagcta aaatttgtga agggatattt gctccttgaa tagtaatcca gaaaatcagc
     1561 cccaatagag caagcataat gggaattccg aatttttttg aagttacaat atcatcaatt
     1621 tttttgtctc tgttaagttt gttaacatcc tcctttacat atttttgttt taatttattg
     1681 gcataattat agttgtcttc tgaaattttt tctcttatac tttcatcctg attaaaatac
     1741 aatgattttt cagtttcctc ttcaatgatt tgtgatgttt tttcaattaa atctttaagc
     1801 tccttcatac cctgaccatt ccttgcggaa gttagtacaa tcggtatttt taattctttt
     1861 tcaagacctt ctttgtcaat tttaatgttt tttttctttg cttcatctat taaattaatg
     1921 caaagtatta ttttattcgt aagttccgac acctgaaaaa ccaagtttaa atttctttca
     1981 agacacgtgg catcagcaac taccacaacc gtatcataat tgccgtaaca tagaaaatct
     2041 cttgtaactt cttcatcttt tgaagcggtg aacaatgaat aagagccggg caggtcaatt
     2101 agtatatatt cattattttt gtatttaaac tggcctcttg cagaaacaac agtcttaccc
     2161 ggccagtttc ccgtatgttg attaagtccc gttaaataat taaaaacagt gcttttgcct
     2221 gtgtttgggt ttcctgccaa ggctactaca tactgtttat catttttttc tatttcataa
     2281 atatctctta atgattccaa ttttacggat tgtgctgtaa gtcccatgct gacaccccct
     2341 atagaacttc aattaaactt gattcttcat ttctaagggc aatcattgct cctcttattc
     2401 tgtaaacggt taaattgttt tttggacctt ttcttaatac ttcaacatct gcaccttttg
     2461 taagtcccaa agcaagaaat ctttccttta aataatcatg agcagagttt tgtttaacct
     2521 ttactgtttt tccaacacca acatcagcta atttcatatt taaactcctg taatatttaa
     2581 aatataactg cataatattt tttagaacat gttagccaaa gctaacattc tataaattat
     2641 aatatgacat tgctcgaatt ttgacacaat taggaggcat tatgaaagaa gagttttata
     2701 cactaaaagg ttataaaata aaaaatgaag aaatcctctc ttcctcaatg gaggattata
     2761 tagaaatgat atgcagattg tctgaaaaat cagatgaagt cagagtaagc gagctgtctt
     2821 cagccttgaa tgtgcagcct ccgtcaacca caaaaatgat aagaaggctg gcaaaggaaa
     2881 attatgtaac ctatgaaagg tacggattta ttaaacttac tgaaaaagga agggaagtag
     2941 gcagcaaatt attggaaagg caccggataa tttttgaatt tttagaatgc ataggagtaa
     3001 aggaaaatat tttggagcaa accgaaataa ttgaacatgc ggtaaacgat gaggttctaa
     3061 ataaaataga tgaattgaca caattcttaa aaaaatgtga taaatataaa taagatgctg
     3121 ctataaaaaa tgctcaaatc gtcattctga acgcagtgaa gaatctcata acttaagatc
     3181 cttcgctgcc tatcaggatg acaaaatttt gttagggcat cttatttttt attgttatca
     3241 acgtcttttt ttcgctcttg cacttcaaat tcacccggaa tatttacatt cccgggattg
     3301 gcagatcttc tatatctcat tttcaatttg tttgttaact tcttaaacat aatttcacca
     3361 cctttattat agttttactt aattttagaa atttattact gtttataaaa ctttgaaact
     3421 aaggtttttt aattattatc attaacttat taatcagttt tgaataagat gatgtaagga
     3481 gtcacagtat gacggaggta gatatggtat atatttgtga actgagaaat gtctctaaaa
     3541 gatttcatac aataaataac gagacaaagg caattgatga tatttcattt aaggttaaaa
     3601 aaggggagat agtttctata gtgggacctt ccggctccgg aaagtcaacg gttttatcgc
     3661 ttatagccgg attggagaag ccgtctgagg gggaagttat tgttaatacc gacagtattg
     3721 gttttatgtt tcaaaaagac cagttatatg aatggagaaa tgtatataaa aactgtcttc
     3781 ttggtttaga agtaaaaaaa atggataatg aagaaaacaa gataaaagta aaaagaatgc
     3841 ttgaaaaata tgggttgacc aattttaaga accattaccc cagccaattg tccggaggaa
     3901 tgagacagag ggctgctctg gtgcgcactt tggcaattga gccggagctg cttttattgg
     3961 atgaaccctt ttcggcattg gattaccaga caagactttc aataggtgac gaagtgggga
     4021 caattttaag aaatgaaaaa ataagcacca tacttgtaac tcacgacatt cctgaagcag
     4081 taagcatttc ggacagagtt atagtcttca ctgaaaggcc ggctaaaatt aaaaaggaat
     4141 atgaaataaa atttgaggag tgtccaggtg aaagaacacc tatgaaatgc agaaatacct
     4201 tggcttttgg tgaatatttc agaaatatat ggagggattt agatgtggga aaagaagttt
     4261 gagccggttg caaacatttc agaagagcac aaagaatatt tgaagttaat aaatagggaa
     4321 aaaaacttta ttaggattgc acaaatcttg atttttgtgt ttggaataat attttgggaa
     4381 gtagcagcca ggaaaaattt aatagataca tttctaacaa gcagtccggg agcaattttt
     4441 ggtctgatta tgaagcttgt tgatgatggc agtctgttta aacatttatt tatttcgaca
     4501 atggaaacaa taggcggatt ttttgtaggt actgctggcg gcttattaat tgcaataatc
     4561 ttatggtggt ttagaaaatt ggctaaggta atggaccctt acattgttat tttaaacagc
     4621 cttcctaaaa cggccttagc tcccataata atcttgtggg taggaatggg atattcagga
     4681 ataatagcta cggctgcatc cattt
//
LOCUS       DAOSOW010000100         4703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_18239_length_4703_cov_2.977840, whole genome shotgun sequence.
ACCESSION   DAOSOW010000100 DAOSOW010000000
VERSION     DAOSOW010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18239_length_4703_cov_2.977840"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1553
                     /locus_tag="PLD67_03715"
     CDS             <1..1553
                     /locus_tag="PLD67_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HOT21659.1"
                     /translation="LVIVSVGSVFLLYAFFGNLLPRTSFLFHAPFTFKRIVCTEWLGS
                     EGILGTPIYVSSTFIFLFLVFATFLKKSGVGDWMTNLATAAVGGQVGGPAKTAVIASA
                     LQGTVSGSSVGNVVATGSITIPLMKDTGYEPNFAGAVEAAASTGGQIMPPIMGAAAFI
                     MTEYVGVTYGTVAISAAIPAILYFSGIFLNVHYEALKKGLLGLPKEKRPDGRKLAKEG
                     WFMILPVILIFGLLIGGSSAMKAAMYGIIGCIIIWIINIIKEEKRFDIVKFVKMFIDS
                     LDESARSAITVAITCGTAGVIVGVVTMTGLGLKMADGVVTLAGGNLLLTMIFTMFSSI
                     ILGMGVPTTANYIIQATISAPALVALDVPPIAAHLFVFYFGIVADITPPVALAAFAGA
                     GISGGNPMKTGFTATRLGIAAYIGPYMLVYNPILVLVNTGNYSTPIFILMVIKSAITA
                     IIGMGGLATGFTRYFKTNCKIWESIVLIVAGLLLVDPGTVTDITGGAMMVAIYLIQKR
                     RVAKAAIS"
     gene            1745..2113
                     /locus_tag="PLD67_03720"
     CDS             1745..2113
                     /locus_tag="PLD67_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21660.1"
                     /translation="MSPGEIIYTISLLIALIAVYLMVLIITMNRNIKKVVQIFEEKNA
                     LSAKTAISYTELNIQEQGIIERAIKKRDNRSQALKFLLDGGVVIITGDERYYLNKKKM
                     IEFKKRLNFIGRMMVPDIDN"
     gene            complement(2233..2805)
                     /locus_tag="PLD67_03725"
     CDS             complement(2233..2805)
                     /locus_tag="PLD67_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HOT21661.1"
                     /translation="MFMYEKKLQYPVRVTNKDVRMAKAVLNQYGGPDGELSASMNYLT
                     QRFSMPLNETKAILTDIGTEELGHMEMVATLFKKLIKGATKEELIAAGMGGYFADHET
                     CPFLVDAEGEHWDSKFTQAKGDPIADLYNDMAAEQKARATYENLILLTDDPLVKDTLR
                     WLREREIVHFQRFGEGLRLVEEYTNSKRYY"
     gene            complement(2836..3111)
                     /locus_tag="PLD67_03730"
     CDS             complement(2836..3111)
                     /locus_tag="PLD67_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HOT21662.1"
                     /translation="MADYNRTNQRYNQQQEMLLEVQKLQFAALDTALYLDTHPNDPVA
                     LYRHKMYTDRLKEVKDAYEMQFGPMTIFNHEVGDYWRYINSPWPWEM"
     gene            complement(3114..3317)
                     /locus_tag="PLD67_03735"
     CDS             complement(3114..3317)
                     /locus_tag="PLD67_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HOT21663.1"
                     /translation="MKILIRRDDKNYIPMVPYMPENPKPGYGYVPYQINPRYFNNLTE
                     AFVQGTIFPDLVTPYSEFFQRGV"
     gene            complement(3498..3782)
                     /locus_tag="PLD67_03740"
     CDS             complement(3498..3782)
                     /locus_tag="PLD67_03740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HOT21664.1"
                     /translation="MIKVVAKGYFLEGKAEEAIKLYEELVEKTRKEEGCISYSLFRDI
                     EDDSILTMIEEWESKDALDKHFKTEHFTKLVPMIGKLRKSSEVNIYKLVI"
     gene            3858..3930
                     /locus_tag="PLD67_03745"
     tRNA            3858..3930
                     /locus_tag="PLD67_03745"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3891..3893,aa:Val,seq:tac)
     misc_binding    4117..4326
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4456..>4703
                     /locus_tag="PLD67_03750"
     CDS             4456..>4703
                     /locus_tag="PLD67_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOT21665.1"
                     /translation="MKKEKVVLAYSGGLDTSIIIPWLKENYNDLDIIACCVNVGQNDD
                     MEFVKNKALSSGASKVYIENAVNEFVEEYVYKGIKANAA"
BASE COUNT         1412 a          800 c          895 g         1596 t
ORIGIN      
        1 gcctggtaat tgtatctgta ggaagtgtgt ttctgctata tgcatttttt ggcaacctac
       61 tgcctagaac ttcatttctc ttccatgccc cgtttacttt taaacgcata gtctgtacgg
      121 aatggctggg aagcgagggt attctgggga caccgatata tgtatcatca acttttattt
      181 tcttattttt ggtgtttgca accttcttga agaagagcgg tgtaggtgat tggatgacca
      241 accttgctac agcagctgtt ggcggtcaag taggcggtcc tgcgaaaact gccgtaatag
      301 caagtgctct gcagggaact gttagtggtt cttccgttgg aaacgttgtt gcaaccggct
      361 ctataacaat ccctttgatg aaagatacgg ggtatgagcc aaattttgcc ggagcggttg
      421 aagcagctgc ttcgacaggc gggcagatta tgccgccaat tatgggtgct gctgcgttta
      481 ttatgacaga atacgtaggt gtaacatatg gtacagttgc catatctgct gctataccgg
      541 caatattgta tttctccggt atatttttga acgttcacta tgaagctctt aaaaaaggtt
      601 tacttggtct tcctaaggag aagcgccctg atggcagaaa gttggcaaaa gaaggttggt
      661 tcatgattct gcctgtcatt ctgatatttg ggttgcttat tggtggaagt tcagcgatga
      721 aagcagctat gtatggtatc ataggatgca taattatatg gattattaat attattaaag
      781 aagaaaaaag atttgatatt gtcaagtttg tgaaaatgtt catagattct cttgatgaga
      841 gtgcccgtag tgccatcacc gtagctatta cctgcggtac cgcaggtgtt atcgttggtg
      901 tcgttactat gacaggtctt ggtctcaaga tggcagacgg tgtggttaca ttagccggag
      961 gcaacttact tcttacaatg atctttacaa tgttcagctc aatcatactt ggaatgggtg
     1021 ttccgactac tgcaaattat atcattcaag ctacgatttc tgctccggca cttgtagcat
     1081 tagatgttcc accaattgct gcccacttgt ttgtattcta ttttggtata gttgctgata
     1141 taactcctcc cgttgcttta gctgctttcg ccggtgccgg tatatccggt ggaaatccga
     1201 tgaagacagg ttttactgca actcgacttg gcatagcagc ttatattgga ccgtatatgt
     1261 tggtttataa ccctattttg gtacttgtaa acaccggaaa ttattcaact cctatcttca
     1321 tcctgatggt aataaaatct gctataactg ctattatagg catgggcggt cttgctaccg
     1381 gatttacccg atactttaaa acaaactgca agatttggga gagtattgtt ttaattgttg
     1441 caggcttact gcttgtcgac cccggtacag ttactgatat tacaggtgga gctatgatgg
     1501 ttgcgattta cttaatacag aaaagacgtg tcgcaaaggc tgcaatttcg tagtatttga
     1561 tatataattg atgtacaaat tcaatctttc atcttgtctc gaatctcgaa ggaggttgtg
     1621 taatgcaatc tcctgttttt atctttacga atggaaacac tatactgaaa cagaaggttt
     1681 gacagggagt gtttgctgtg ttaaactgaa aaaaatatta tactaacaaa cggaggctgt
     1741 tctaatgtca ccaggagaaa ttatttatac aataagtcta ttaatcgcac ttattgctgt
     1801 ttaccttatg gtattaataa tcaccatgaa tagaaatatt aaaaaggtag ttcaaatatt
     1861 cgaagagaaa aatgccttat ctgcaaaaac agctataagt tacacagaac ttaatattca
     1921 ggaacagggt attattgaac gtgccataaa aaaacgggac aatcgctctc aagccctgaa
     1981 gtttctgttg gatggaggag ttgtgattat tacaggtgac gaaaggtact atctaaataa
     2041 aaagaaaatg attgaattta aaaaacgttt gaattttatc ggacgtatga tggttcctga
     2101 tatagacaat tgacgaatac cctttaaaaa taatcttcag tttaatatat acagtaataa
     2161 aaacattata tttgcaaaaa tcacctatga gaacaagatc ctcataggtg attttatcaa
     2221 ttaaaaatat aactaataat atctcttgct gttagtatat tcttctacta gccttaatcc
     2281 ttctccaaat ctttggaagt ggactatttc acgttctctc agccatctta aagtatcttt
     2341 tactaagggg tcatcggtta ataatattaa gttttcgtag gttgctcttg ctttttgctc
     2401 agcagccata tcgttgtata agtctgctat aggatctcct ttcgcctgtg taaattttga
     2461 atcccagtgt tctccttccg catcaactaa gaagggacat gtttcatggt cagcaaaata
     2521 ccctcccata ccggctgcaa ttaattcctc cttagtggca ccttttatta actttttaaa
     2581 taaggtagca accatctcca tatggcctag ttcttcggtg ccgatgtcag ttagtattgc
     2641 ttttgtttca tttagaggca tgctgaatct ttgagtaaga taattcatac tggcgcttaa
     2701 ttcaccgtca ggaccgccgt attgatttaa aacagcttta gccattcgaa cgtctttatt
     2761 agtaactctt acaggatact gcaatttttt ttcatacata aacatagagt tatcattcct
     2821 ttctatataa tctacttaca tttcccaagg ccacgggctg tttatatatc gccaatagtc
     2881 accaacttca tggttaaata tagtcattgg tccaaattgc atttcatatg catccttaac
     2941 ttcttttaac ctgtcggtgt acattttatg tctatacagg gcaacggggt cattcgggtg
     3001 agtatctaaa taaagtgcag tatctaatgc tgcaaattga agtttttgta cttccaacaa
     3061 catttcctgc tgttgattat atctttgatt tgttctgtta taatctgcca taattacacc
     3121 cctctttgaa aaaattctga atatggagta accaaatccg gaaatatcgt accctgaaca
     3181 aaagcctcag ttaaattgtt aaaataacga ggatttattt gataaggaac atatccgtat
     3241 cccggttttg ggttttcagg catatatggc accatcggta tataattttt atcatctctt
     3301 ctaatcaaaa ttttcattcc tttcctatga atatttgcta tttcgttaaa aaaatagtta
     3361 tttcatatta ttcataaata gacaaaaagt gacgcaattt tcaaatgttg ttaatgaact
     3421 attctcattt ttagtctcta tttattataa aaaatacaag atgattcggt tgaatcatct
     3481 tgtataagaa ggtattctta aattactaat ttataaatat ttacttcact gctttttctt
     3541 agttttccta tcattggaac cagtttcgtg aaatgttctg ttttaaaatg tttatccaag
     3601 gcatctttgc tctcccattc ctctatcatt gttagtattg aatcatcttc aatatccctg
     3661 aataaactgt aggaaatgca tccttcttct tttctggttt tttcaactaa ctcttcgtat
     3721 agttttattg cttcttctgc tttgccttct aaaaagtaac ctttagctac tactttaatc
     3781 atacgtcctc cttatttttt tataaaatat taaaaatgtt ttgacaacct tatgaagttg
     3841 tggtattttt attatacggg agcttagctc agctgggaga gcatctgcct tacaagcagg
     3901 gggtcatagg ttcgagccct atagctccca ttaatatacg gcatgtgccg tatttttaat
     3961 tatactatat attatcataa aaatcggatc taagtaaatc taaaattaac gaatattccg
     4021 gttaaagaat aaatataata attttagaaa aaaaataaaa aaatatgttg acaaaataag
     4081 aaaagcaaca tataatttta gaaaaattat taattgtata attttatgat tggaagagta
     4141 gattaaataa agcttcagcg agccggagca gagtgtgagt tcggtagtgt agtttaattg
     4201 aaaagagtcc atgaaaagat tctcagaagt agcagtatga gaatacggtt aaagaccgtt
     4261 atcatgaaag agcgaagcag ctgctttata aaagagtggt accgcggtgt aaacactgcc
     4321 tctttatagg cagttttttt ttgccctgac acgttgatga aggtattggg acacagctct
     4381 gaagtgagga tagtctttga agaaagcata cattgcaatt ataattagtt ttatttaaat
     4441 ttggaggagc agaaaatgaa aaaagaaaaa gtggtattag catattcagg aggattggac
     4501 acatcaataa taatcccttg gctgaaagag aattacaatg atttagatat tattgcttgc
     4561 tgcgtaaatg taggtcagaa cgatgatatg gaatttgtta agaataaagc attatcttcc
     4621 ggtgcaagca aggtatatat cgaaaatgca gtaaatgagt ttgttgaaga atatgtttat
     4681 aaaggaatta aggcaaatgc ggc
//
LOCUS       DAOSOW010000101         4695 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_18274_length_4695_cov_3.132112, whole genome shotgun sequence.
ACCESSION   DAOSOW010000101 DAOSOW010000000
VERSION     DAOSOW010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4695)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4695
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18274_length_4695_cov_3.132112"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLD67_03755"
     CDS             <1..251
                     /locus_tag="PLD67_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21666.1"
                     /translation="NTCGATMSSFLGVSTFAYAPFAFFNLLSPIVSVIYGFTGFSIMT
                     IEEDPSSPEYKQKMKVKKSPKELEEYIANYQAKARLAE"
     gene            365..2452
                     /locus_tag="PLD67_03760"
     CDS             365..2452
                     /locus_tag="PLD67_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT21667.1"
                     /translation="MGYGSMFEKELNKLIESEDNIECIKDIISNDISNTEQNKEYIER
                     LLEFSTENKLYRSEAWGYYFKGWHYIDNSEYEKAVENFMISYELFDKLNNKYEIAYAC
                     NGLTNVYCLMGQYKLSNEWGLKGISFCEETKNSKAMIILLINTCINYIQMEYYDKALE
                     IIESIEEIDYDLTKSQEISHMLAKAEIEINIGNPDKALKNIEQAMKIESVNNIKSTMG
                     EMLKLKGMAYLKTGKYDLAEDQFKKSYNFCVKYDLIYEKCSTMLQWSKLCILTDNYEK
                     AIDLLNQIIIVGSSNKFNTIMREAFHSLYSIYKKLEDTEKALNYLEKYIKINNEMYDY
                     EQSQLMAEINLNNTKRMAEQYKQLYKRTELLSTIGQKIISNLDISSIIRIINEEIKKL
                     IDLDYFGIAVYDSENNRSEYYFMRDYKIVCSTVNYDEENTLGGYCIKNKEDIIIGNEK
                     LEFKKYLYSEPNYIRGFEEEGVKISSIIYTPMIINDKVVGLMTAQSEKENLYGKDELN
                     ILKILANYTAIAVENAKVYKEIENIATYDSLTNFLTKNEILKIGDSIYEKAKCNNQKF
                     TVIMMDLDDFKIVNDTYGHVYGDKAMSLIAETMSKCIRNTDYIGRFGGDEFLLICTGT
                     GLEEALYVAERIRKTIQSSIFVLSDEIKVRVTTSLGVHEYDENDNSFTDVVKRADQCL
                     YLAKETRNTVMSK"
     gene            2608..4308
                     /locus_tag="PLD67_03765"
     CDS             2608..4308
                     /locus_tag="PLD67_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOT21668.1"
                     /translation="MKKILRINLRTKQITYQDASREYLETGGRGLIAKIMLNEVNPLC
                     DPLGRENKLIIANGLLTGTNVSSASRIAIGAKSPLTGGIKESNGGGITAMWLASLGIK
                     ALVVEDIPEEDEWYYIRINKDNCSLEPAGEYKGMGTFEFCEKMLDKYPDCAVTCIGQA
                     GERLYNIAGIATMDKEKKPNRYSGRGGLGAVMGSKKLKGIVVEEKGTVEIKNKEKYNE
                     ILKEYSEVVLNATTSVSYKTLGTAATVRTTNDLTGLPVTNFKGAKFEHAEEISGESLH
                     RLIKERGGEGKTAHACMPGCIIQCSNVVPDEEGKTIVSPLEYETIGLMGSNLGIGDLD
                     TIAKLNAVCNDLGVDTIETGAAMGMAMEAGIVEYGDNEVALNLLDEIRAGTELGKLLA
                     EGTLIAGKALGVKNIPVINGQAMPAYDPRAIKAMGVTFATSPMGADHTYGPVIKSEDA
                     VEASKAAQIMMAKIDCLGVCMFLRGAVAKHMDLFVDLVNAVHGWDLDMDWLGDIAMEA
                     IRDEHKFNELAGFTKEDYRMPKAFTNREIESTNQVFDIDKDELDELRLWHDKNKIIYR
                     "
     gene            4280..4492
                     /locus_tag="PLD67_03770"
     CDS             4280..4492
                     /locus_tag="PLD67_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21669.1"
                     /translation="MIKIKLSIDDSDVENIDLDYKDITIDEIITELKIDKQHIGAVLV
                     NGIPKKFSDKVEDNSEVFFLPILSGG"
     gene            complement(4536..4673)
                     /locus_tag="PLD67_03775"
     CDS             complement(4536..4673)
                     /locus_tag="PLD67_03775"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HOT21670.1"
                     /translation="MGIKIIYGLKKPAINKQWFGFHELFHIFVLAGSFCHFMMMYFYV
                     A"
BASE COUNT         1779 a          652 c          976 g         1288 t
ORIGIN      
        1 ggaatacatg cggggctaca atgtcttcat tcctgggtgt aagtacattt gcatacgcac
       61 catttgcatt ctttaacctt ttaagcccga ttgtatcagt tatttacggt ttcaccggat
      121 tctcaattat gacaattgag gaagacccgt catcacctga atataagcaa aaaatgaagg
      181 ttaagaaatc accgaaagaa ttggaagagt atatagcaaa ttatcaagct aaagcaagat
      241 tagctgaata ataaagaaaa tcaaaaacct tgcgggagtc cgcaaggttt ttttgtaata
      301 agtatttgac aatttttagc gttcaatata ttatatttct aatcgattct caaggaggat
      361 aaaattgggt tacgggagta tgtttgaaaa agaattaaat aaactgattg aaagcgaaga
      421 taatattgaa tgcataaaag atataatttc gaatgacata agcaatacag agcagaataa
      481 agaatatatt gaaagattgt tggaattttc cacagagaat aagctataca gatccgaagc
      541 gtggggttat tattttaaag gctggcatta tatcgataat tctgaatatg aaaaagcagt
      601 agaaaatttt atgatttctt atgagctgtt cgataagcta aacaataaat atgaaatagc
      661 gtatgcctgc aatggattga caaatgttta ttgtttgatg ggacagtata aattatcaaa
      721 tgaatggggt cttaaaggca tttcgttttg tgaagaaacg aaaaactcta aggcaatgat
      781 tatccttctt atcaatacat gtattaacta tattcaaatg gagtattatg acaaagcgtt
      841 agaaattata gaaagtattg aagaaataga ttatgattta acaaaaagtc aagaaatttc
      901 gcacatgctg gcaaaagccg aaattgaaat aaatatcggc aaccccgaca aagcccttaa
      961 aaatattgag caagcgatga aaatagaatc agtaaataat attaaatcta ccatgggtga
     1021 aatgttaaaa cttaagggta tggcttattt aaaaacaggt aaatatgatt tagctgaaga
     1081 ccagtttaaa aagtcttata atttttgtgt taaatatgat ttgatatatg aaaagtgctc
     1141 caccatgctg cagtggtcta aattatgtat tttgactgat aattatgaaa aggcaattga
     1201 cttattaaat caaattatta ttgtcggttc atcaaataag tttaacacaa taatgagaga
     1261 agcatttcat tctttatatt ctatttataa aaaactggaa gatactgaaa aggcattgaa
     1321 ttaccttgaa aagtatataa agataaataa tgaaatgtat gattatgaac aaagccagct
     1381 tatggctgaa ataaatttga acaatacaaa acgtatggca gaacaatata aacagcttta
     1441 taaaaggact gaacttcttt caaccatagg gcagaaaata atatcaaatt tagatattag
     1501 ttcaattatc cgcatcataa acgaagaaat aaaaaagctg attgatctcg attattttgg
     1561 aattgctgtg tatgacagtg aaaataaccg gtcggaatat tattttatga gagactataa
     1621 aatagtttgc agcactgtaa attatgatga ggaaaatact ctgggaggat attgtattaa
     1681 aaataaggaa gatattatta tcggaaatga aaagctggaa tttaagaaat acttatattc
     1741 tgaacccaac tatatcagag gatttgaaga ggaaggcgta aaaatttctt ccattattta
     1801 tactcccatg attataaatg acaaagttgt cggattgatg actgctcaaa gtgaaaaaga
     1861 aaatctttac ggcaaggatg aattgaatat cttgaaaatt ctagcaaatt atactgcaat
     1921 tgctgttgaa aatgccaagg tatacaaaga aatcgagaat attgcaactt atgacagtct
     1981 gacaaatttt ctgactaaaa atgaaatact gaaaattgga gatagtattt acgaaaaggc
     2041 caagtgcaac aatcaaaaat ttaccgtaat tatgatggat ttggatgact ttaaaatagt
     2101 aaatgataca tacggtcatg tttacggaga taaagcaatg agtcttattg ccgaaaccat
     2161 gtcaaaatgc attaggaata ctgactatat aggaagattc ggaggagatg agtttttgct
     2221 catatgtacc ggaacaggat tagaagaagc cttatacgtt gccgaaagaa taagaaaaac
     2281 aatacaaagc agtatatttg tattaagtga cgaaataaaa gtaagggtaa caacgagttt
     2341 aggggtacat gaatatgacg agaatgacaa ctcatttacc gatgtggtaa aaagagcaga
     2401 ccaatgcctc tatcttgcaa aagaaacaag aaataccgtt atgagtaaat aaataacaaa
     2461 cccggagggg aaaacccgga ggggacggtt ctcagtggac aaccgtcccc tttgggtcca
     2521 aattagacat aacagtgtca aaattattat gattaaattt attgctgagg ggtacactaa
     2581 ttttgacatt ttaaatagga gagcaatatg aaaaaaattc tgagaataaa tttaagaaca
     2641 aaacaaatta cttaccaaga tgcttctcgg gaatatttag aaacaggagg caggggacta
     2701 atagcaaaaa taatgctaaa tgaagtaaat cctctatgcg accccttagg cagagaaaat
     2761 aaacttatta ttgccaacgg actattgacc ggtacaaatg tgtccagtgc aagcaggatt
     2821 gctatcggag ctaaaagtcc cttaacaggc ggaatcaagg aaagcaacgg aggaggaatt
     2881 acagccatgt ggcttgcttc ccttgggata aaagctcttg ttgttgaaga tattcctgaa
     2941 gaagatgaat ggtattatat aagaatcaat aaggataatt gctccttaga gcctgcagga
     3001 gaatataaag gaatgggaac ctttgaattt tgcgagaaaa tgcttgataa atatcctgac
     3061 tgtgcagtta catgcatagg ccaggcaggc gagaggttgt acaatattgc cggaatagca
     3121 actatggata aggagaaaaa accaaacaga tacagcggca ggggcggtct tggtgctgtg
     3181 atgggttcaa agaaactaaa aggaatagta gtagaagaaa aagggacggt agaaattaaa
     3241 aataaagaaa aatacaacga aatactaaag gaatattcag aagtagtttt gaatgctact
     3301 acatccgtat catataaaac attagggaca gcagcaacgg tgaggactac aaatgatttg
     3361 acaggacttc ccgtaacaaa tttcaaaggt gcaaaatttg agcatgcaga agaaatttca
     3421 ggagagagct tacataggct tataaaagaa agaggcggag aaggaaaaac tgctcatgcg
     3481 tgtatgccgg gctgcataat tcaatgctct aatgttgttc ccgacgaaga aggaaaaaca
     3541 atcgtatctc ccttggaata tgaaacaatc gggttaatgg gctccaatct tggaattgga
     3601 gatttagaca caattgcaaa actaaatgcg gtctgcaatg atttaggtgt tgacaccata
     3661 gagacgggag ctgccatggg aatggcaatg gaagcaggaa ttgtggaata tggcgataat
     3721 gaagtggctt taaatttgct tgatgaaata agagccggta cagagctcgg aaaattgtta
     3781 gcagaaggaa cacttattgc gggcaaggca ttgggagtta aaaatattcc tgttataaac
     3841 ggtcaagcaa tgcctgctta tgacccgaga gcaataaagg caatgggagt tacttttgca
     3901 acatcaccca tgggtgctga ccatacttac ggtcccgtta taaagtcgga ggatgctgtc
     3961 gaagcatcaa aagcagcaca aattatgatg gcaaaaatag actgtttggg agtatgtatg
     4021 tttttaagag gagctgttgc aaaacatatg gacctattcg tggacttggt aaatgcagtg
     4081 cacggctggg atttagatat ggattggcta ggggatatag ctatggaagc gataagagat
     4141 gagcataaat ttaatgagct tgcaggtttt accaaagaag actatcgtat gccaaaggca
     4201 tttacaaaca gggaaattga aagtacgaat caagtttttg atattgataa ggatgaactg
     4261 gatgagttga ggttgtggca tgataaaaat aaaattatct atcgatgaca gcgatgtgga
     4321 gaatattgat ttagactata aagatattac gattgatgaa ataattacgg aattaaaaat
     4381 tgacaagcag cacataggtg ctgtgcttgt caacggtatt ccaaagaaat tctcagataa
     4441 agtcgaagat aattcagaag tattcttcct tcccatactg agcggcggct agtagcctga
     4501 gatccttcac ttatgctcag gatgacatta ataaattaag ctacataaaa atacatcatc
     4561 ataaagtgac agaaggaacc tgctaataca aatatatgaa acaattcgtg aaacccgaac
     4621 cattgtttat ttatggcggg tttttttaaa ccgtaaatta ttttaattcc caaaaaaaat
     4681 ttttaaataa tctta
//
LOCUS       DAOSOW010000102         4642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_18564_length_4642_cov_2.674079, whole genome shotgun sequence.
ACCESSION   DAOSOW010000102 DAOSOW010000000
VERSION     DAOSOW010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18564_length_4642_cov_2.674079"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="PLD67_03780"
     CDS             <1..631
                     /locus_tag="PLD67_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT21671.1"
                     /translation="EVDRINSQKQHIVVLTGDLIDKYNEYNDKDNLNKIWEVLSEIKA
                     PLGKYAVYGNHDYGGGAERAYKQIMEKSGFKVLLNKNEKLEEYNINIIGMDDSIFGTK
                     DNKAIVDYLDENYFNIILSHEPDVAEYFLEYDADLFLTGHSHGGQINLPFINYTPPLA
                     KKYVRGMYEVSNYRQTKIYVNVGLGTSQIPMRFMATPELTVITLKGTSS"
     gene            complement(723..2453)
                     /locus_tag="PLD67_03785"
     CDS             complement(723..2453)
                     /locus_tag="PLD67_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HOT21672.1"
                     /translation="MKKVLSLVLVVLLILSSFSFVFAEDAETVKITVIHTNDTHSRLI
                     GTNTEIGFAKIATLIKEAKEANPNTLVLDAGDTLHGMPIVNISKGENAIKVLEAAGYD
                     YMTLGNHDFNYGKERLFELRDMSQVGMLSANIVDENGEYIFTPYVIEEVGDVKVGIYG
                     LSTPDTVILTNPNNVVGLVFKDPIEVSKEMVEELEDKTDVIIALAHLGLDESSTLTSK
                     ALAQEVEGIDLIIDGHSHSMLEAGQLENNTLIVQTKNYGQNLGYIDIEILEGEVTGIT
                     ARLLAAADTADVVPDPDLQKIIEDIEAANAEVFNEVIATTGVYLDGVRENVRTKETNL
                     GNLSADAARAATGADIAFVNGGGIREDIPVGDITKGKIAAIFPFGNTIEVKKITGADL
                     KAMLEWSVSDYPAAKGAFLQVSGLEFTFDPAKEIGSKVVEILVGGEAFDEAKEYTVAI
                     NDFMSTGGDGYAMLADYDVLAIYGTYEEIIIDYLVANGTAGSEVSGRIKVMETVVEEL
                     EPVEPEPVEPAPVEPEPVEEVIYIVVPGDVLWKIAAKYNLTYQKLAEYNNIANPHLIF
                     PDQVIKVPTK"
     misc_binding    2709..2906
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2975..4420
                     /gene="proS"
                     /locus_tag="PLD67_03790"
     CDS             2975..4420
                     /gene="proS"
                     /locus_tag="PLD67_03790"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOT21673.1"
                     /translation="MAKKEKEFVKEITPMEEDFSRWYTDVIMKNELVDYSPVKGFMVI
                     RPYGYALWEKIQEYADGKFKATGHKNMYFPLLIPESLLNKEKEHVEGFAPEVAWVTQG
                     GNQELTERLVIRPTSETIICHMYAKWLNSYRDLPYLYNQWCSVVRWEKTTRPFLRTSE
                     FLWQEGHTLHETAEEAQAETLQMLDIYRQVAGDLMAMPVVVGQKSEKEKFAGAYATYT
                     MEALMHDGQALQAGTSHNLGQHFAKAFEIKFQGRNGQEQYPYSTSWGISTRLIGGLIM
                     VHSDNRGLVLPPKMAPVQVVIIPIAQKKEGVIEKANELFNRLKAKGIRVELDDNTEYS
                     PGWKFNQYEMKGYPIRIEIGPRDIENNQAVAVRRDELEKVILSLDNIEDTIYNMLETM
                     QNDLFEKAKAHREANTYVVEDYEKYKRDLPNRPGFAKMMWCGERECEDKLKEETGATI
                     RCIPFEQENLGDKCHICGKPAKHMIYAARAY"
BASE COUNT         1550 a          774 c          879 g         1439 t
ORIGIN      
        1 agaagtcgat agaataaaca gccaaaaaca gcatattgta gttttaacgg gtgatttgat
       61 agataaatac aatgaataca atgataaaga caatttaaat aaaatatggg aagttttaag
      121 cgaaataaaa gctcctttag ggaagtatgc agtatatggc aaccatgatt acggcggcgg
      181 cgcagaaaga gcctataagc aaattatgga gaaaagcggt tttaaagtgc tattaaacaa
      241 aaatgaaaag cttgaagaat ataatattaa tataataggg atggatgatt caatttttgg
      301 aacaaaagac aataaagcca tagtcgacta tttggatgaa aattatttca atataatatt
      361 aagtcatgag ccggatgtag cggaatattt tctggaatat gatgcagatt tgtttttaac
      421 cggacacagc cacggaggac aaataaacct gccatttatt aattacactc cgcccttggc
      481 aaaaaagtat gtaagaggga tgtatgaggt ttccaattac aggcagacga aaatatatgt
      541 aaatgtaggt ttgggaacat ctcaaatacc gatgcgtttt atggcaacac cggaattgac
      601 ggttattact ttaaaaggca catcatcatg agatccttcg cttacgctaa ggatgacata
      661 aaagggtttg taaaaaaaat tgaaggcttt gttatgttaa caaagccttc atatatctca
      721 ttttacttgg ttggaacttt tattacttgg tctgggaaaa ttaaatgagg atttgctata
      781 ttgttgtatt cagccaattt ctgataagtt aaattatatt ttgctgctat tttccataga
      841 acatctcccg gtacaactat gtaaatcact tcttcaacag gttcaggctc tacaggagca
      901 ggttcaacag gttcaggctc tacaggttca agttcttcaa caacagtttc cataacctta
      961 attcttcctg aaacttcact tcctgcagta ccgttggcaa caagataatc tatgatgatt
     1021 tcttcatagg taccgtatat tgcaagaaca tcataatcag ccagcattgc atatccgtct
     1081 ccgccggttg acataaagtc atttatagca accgtatatt ccttagcttc atcaaatgct
     1141 tctccgccga ctaatatttc tacaaccttg gaaccaattt ctttagcagg gtcaaatgta
     1201 aattccaatc cgcttacttg caggaaggct ccctttgctg caggataatc gcttacagac
     1261 cattcaagca ttgctttcag gtcagcacct gtaattttct ttacttcaat tgtattgcca
     1321 aatgggaata tagctgctat tttacctttt gttatatcac caacaggtat atcttctctt
     1381 atgccgccgc cgtttacaaa ggcaatatca gcacctgttg cagcccttgc tgcatcagca
     1441 gataggtttc ctaaatttgt ttccttagtt ctgacatttt ctcttactcc gtctaagtat
     1501 acgcctgttg ttgctataac ctcattaaat acttctgcgt tggcagcctc aatatcttct
     1561 attatttttt gcaaatcagg gtcgggaaca acatctgcag tgtctgcagc tgcaagaagt
     1621 cttgctgtaa tacctgtaac ttcaccttct aaaatttcta tatcgatata acctaaattt
     1681 tgaccgtaat tttttgtttg aacaatcaat gtattatttt ctaattgacc ggcttctaac
     1741 attgaatggc tgtgtccgtc tattatcaaa tcgattcctt ctacttcttg agctaaagcc
     1801 tttgatgtta aagttgaact ttcatcaaga cccaaatgag ccaatgcaat tataacatct
     1861 gttttgtctt caagttcttc taccatttcc tttgatactt ctataggatc tttaaatact
     1921 aaaccaacaa cattattagg atttgtaaga attactgtat caggtgtaga aagaccgtaa
     1981 ataccaacct taacatcgcc tacttcttca ataacataag gagtaaaaat atattctccg
     2041 ttttcatcaa caatattggc gctcaacatt cctacctgag acatatctct taattcaaac
     2101 aatctttctt tgccgtaatt aaagtcatgg tttcctaatg tcatgtaatc ataacctgct
     2161 gcttccaata ccttaattgc attttcacct ttggagatat ttactatcgg cataccgtgg
     2221 agtgtatctc ctgcatcaag aaccagggtg tttggatttg cttcttttgc ttctttaata
     2281 agagttgcaa ttttagcaaa gcctatttca gtatttgttc ctattaatct tgaatgggta
     2341 tcattagtgt gaataacagt aatctttacg gtttcagcat cttctgcaaa tacaaagctg
     2401 aaactgctta aaatcagtaa tacaactaat actaaactta atactttctt cattaaattt
     2461 tcctcctttt tgttatgata agacttatgt ccattataca gtcaggaggc tggaatttca
     2521 agacaacttt tgaataattt gcaaatttga gagtcactgt acttttaatc agcctgtcat
     2581 tgttattaat aacattcgta tttatatcgt ggtgtggtaa atacgtgata ctgaacaaag
     2641 ttagcataaa aaatatctta aatctgttga caaagtttat atatttttat attatgtata
     2701 caaattaata atttgcgatg aagaggaata gtaaatttac acggtctcta gagaggaaat
     2761 catcggtgga agattttcga ccccctgtat tttgaacccg ccttggagta tttgagggta
     2821 aaaactcaaa gggaagtccc ttatatacaa tgagtgctgt ttttaaaaca gtaattagag
     2881 tggtaccgcg gatatccgcc tcttacggag gcggttttat ttttgtaagg ttatgaattt
     2941 ggagttatga gtctgtgtta cgaaaggaga aggtatggct aagaaagaaa aagaatttgt
     3001 aaaagaaatt acgcctatgg aagaggattt cagcaggtgg tatacagatg taataatgaa
     3061 aaacgaattg gttgattatt cgccggttaa aggttttatg gttataagac cttacggata
     3121 tgctttgtgg gagaaaattc aagaatatgc ggatggaaaa tttaaagcga caggtcataa
     3181 aaatatgtat tttccattgt taattcctga aagtttgtta aacaaggaaa aagaacatgt
     3241 ggaagggttc gctcctgagg ttgcatgggt tactcaggga ggaaatcagg agttgacaga
     3301 aaggcttgta ataaggccta catcagaaac cattatatgt catatgtatg caaaatggct
     3361 taattcatac agggatttgc catatttgta taaccagtgg tgcagtgttg taaggtggga
     3421 aaaaacaaca agaccttttt taagaacatc ggaatttttg tggcaggaag gtcacaccct
     3481 tcatgaaaca gcagaagaag ctcaagcgga aactttacaa atgcttgata tatatagaca
     3541 ggtggcggga gacttaatgg ctatgcccgt tgtcgtaggt caaaaaagcg aaaaagaaaa
     3601 atttgccggt gcttatgcca catatactat ggaagcattg atgcatgacg gtcaggctct
     3661 gcaggcggga acatctcata atttagggca gcattttgca aaagcttttg aaattaaatt
     3721 tcaaggcaga aacgggcaag agcaatatcc ctattccacg tcgtggggaa tttccactag
     3781 attaatcggt ggtctaataa tggttcattc tgataacagg gggttggtac ttcctcctaa
     3841 aatggcaccc gtgcaggttg taataattcc aatagctcaa aagaaagaag gcgttatcga
     3901 gaaagctaat gaattattca acagattaaa agcaaaagga ataagagtag agcttgatga
     3961 caatacagaa tattcacccg gatggaaatt caatcaatat gaaatgaagg gttatcctat
     4021 tcgtattgaa ataggaccaa gagatataga aaataatcaa gcagttgcag taagaagaga
     4081 tgaattggaa aaagtaattc tttctctgga taatattgaa gatacaattt ataacatgct
     4141 tgaaacaatg caaaatgatt tatttgaaaa agcaaaagct cacagagaag caaacactta
     4201 tgtagttgaa gattatgaaa agtataagag agatttaccc aaccgtcccg gatttgcaaa
     4261 aatgatgtgg tgcggtgaaa gagaatgcga agataaatta aaagaagaaa ccggtgcaac
     4321 aataagatgt attcctttcg agcaggaaaa cttaggagat aagtgccaca tatgcggaaa
     4381 gcctgcaaaa catatgattt acgcagcaag agcatattag atgtcccaac cttattatag
     4441 aaatccttca cttcttcagg atgactgtca ttcagaacag aggcgaggga tttcgctttt
     4501 tatttaataa attaaatcaa attgatttaa atcaaagaat gacgtcgatt agttttgtac
     4561 aataagggta taaaataata aagtaaatta ggcaattgta aaatgatttt gtgataactg
     4621 tggataaaat ctaataaaaa aa
//
LOCUS       DAOSOW010000103         4618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_18695_length_4618_cov_3.500986, whole genome shotgun sequence.
ACCESSION   DAOSOW010000103 DAOSOW010000000
VERSION     DAOSOW010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18695_length_4618_cov_3.500986"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(360..1013)
                     /locus_tag="PLD67_03795"
     CDS             complement(360..1013)
                     /locus_tag="PLD67_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HOT21674.1"
                     /translation="MSSLVLKITALITMIIDHYGAIFQGNIIIYRIIGRLAFPIYCFL
                     LVEGYTHTSNIKKYASRLIAFAIISEIPFDLAFFGKIGFEHQNIFFTLFIGLAAIYFI
                     DNKEGKYSTNSAFIIIAASVIATLLRTDYQFIGIIYILAFYFTRNQAKHKKFLTIGLI
                     MFAVNLSAGMLQQFSLLALIPIYFYNKKEGRKNKILQISFYTAYPLHLIIFYLINTL"
     gene            complement(1176..1487)
                     /gene="trxA"
                     /locus_tag="PLD67_03800"
     CDS             complement(1176..1487)
                     /gene="trxA"
                     /locus_tag="PLD67_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOT21675.1"
                     /translation="MNTVKITSDNFEQEVLKSDKPVLLDFWAAWCGPCKMISPIIEEI
                     AKEVTDAKIGKVNIDEQPELASAFGIMSIPTLVKIKDGKVVNKMIGVRPKASIVQMLA
                     S"
     gene            1725..2024
                     /locus_tag="PLD67_03805"
     CDS             1725..2024
                     /locus_tag="PLD67_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21676.1"
                     /translation="MEKLFLQTENGLIPLDEKMIEKYNLVKGRKSPFTRYYVVDETGN
                     HNLGSTKETSSDDVDRMPAGEGAEDDEIVEFSDTGTILSQSEIIDFSHGTDSEEP"
     gene            2113..2484
                     /locus_tag="PLD67_03810"
     CDS             2113..2484
                     /locus_tag="PLD67_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT21677.1"
                     /translation="MSSNEGFVCDCEVIHGDIVNEVKKRMPDDEDIYDLSDFFKVLGD
                     STRMRIIWALDESEMCVCDIAVLLNMTKSAISHQLRALRNANLVKFRKEGKTVYYSLK
                     DKHIRDIYEIGMEHIKEKYNK"
     assembly_gap    2574..2673
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2674..4441
                     /locus_tag="PLD67_03815"
     CDS             <2674..4441
                     /locus_tag="PLD67_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOT21678.1"
                     /translation="SQLKLIMYIISYILAGGDVVKRAIENIKNGQVFDENFLMSAASI
                     GAFFIGEAPEGVGVMLFYQIGEMLQSYAVGQSRRSISSLMDIRPDYANVLRNGEVLTI
                     SPEEVEIGEIIVIKAGERVPLDGIVVEGNSMLDTSALTGESLPREVITGSELLSGCIN
                     MNGLLNAKVTKSYSESTVNKILELVENASSRKSKSEKFITRFARVYTPVVVVIAVVLA
                     IIPPLIIKGATFNEWIYRSLAFLVVSCPCALVISVPLSFFGGIGGASRNGILVKGSNY
                     LESLAKTEIIVFDKTGTLTKGVFKVQKIHSEKIKPEELLKLAAYAESYSNHPISLSLK
                     EAYKKDIDIEKISQVEEISGHGVSAAVENIKVLAGNDKLMEKFKIPYNKETSEGTLVH
                     IAADGEYAGYILIADKIKDDAFEAVSDLRKHNIKDIVMLTGDSESVAINTARELGIDR
                     VYAGLLPADKVEKVEELISQKSKKVTLMFVGDGINDAPVLARADVGVAMGALGSDAAI
                     EAADVVIMTDEPSKIAKAIEIAKNTIKIAYQNVIFAISVKVIILVLSTLGLASLWAAV
                     FADVGVTFIAVLNAMRALRLNN"
BASE COUNT         1545 a          654 c          865 g         1454 t
ORIGIN      
        1 cttctaaatt atttcctgtt gcacttacac atattttcat tttttgtttc tccttctata
       61 tatattcttt taccccttac acttcagttg aagcgtgtaa cttattttta gttactgacg
      121 aattatagca cgatactttt cgtaatgaaa ccctcaatat tatgttttat tggtaaatgt
      181 aacaaatata ttacacaatt atgaagcaaa agtcatatat tacaagttta ttacaaaaat
      241 taaactaaat tattgcatga ctggtaatta ttataaacaa ttattgaata tgagactgct
      301 tcccggggcg ggttcggaga gaggatttat gtgaaaggtt aaaatatttg agctatgttt
      361 tataatgtat ttattagata aaatattatc agatgcaagg ggtaagcagt gtaaaaactt
      421 atctgaagaa ttttgttttt tcttccttcc ttcttgttat agaaatagat tggtattaat
      481 gcaagcaaag agaattgttg cagcattcct gccgaaagat ttactgcaaa cataattaat
      541 cctatagtga gaaatttttt gtgctttgcc tgatttctag taaaataaaa tgctaaaata
      601 taaataatac ctataaattg ataatccgtt ctaagcaagg ttgcaatgac agatgctgca
      661 ataataataa aagcactatt ggtgctatat tttccttcct tgttatcaat aaaatatatg
      721 gcagctaaac ctataaagag agtaaaaaat atattttgat gctcaaaacc gattttaccg
      781 aaaaatgcca aatcaaaagg tatttccgaa atgatagcaa atgctatcag tcttgatgca
      841 tatttcttta tattgctagt gtgggtatat ccttcaacca ataagaaaca ataaatcggg
      901 aatgctaatc ttcctattat tctgtaaata attatattac cttgaaaaat tgctccgtaa
      961 tgatcgatta tcattgtgat tagtgccgtt atttttaaaa ccagactgct catgattttc
     1021 tccatttttg tgttttcaat tgattatatc aatttatatt tataatttca acaaaaaagg
     1081 cagctactgc tgccttttgc gaggttccat atagggggtt taattattgg gttctttttt
     1141 tggtgtaaaa tattatattt ttggaggaga aaattctatg atgctaacat ttgtactatt
     1201 gaagctttag gtcttactcc aatcattttg ttaacaactt tcccgtcttt aatttttacc
     1261 aatgttggta tactcataat gccaaatgct gatgctaatt ccggctgctc atctatatta
     1321 accttgccta tttttgcatc ggttacttct tttgctatct cctctattat cggagaaatc
     1381 attttgcagg gaccgcacca ggctgcccag aaatctaaga gtaccggttt atctgatttt
     1441 aaaacttctt gctcgaagtt gtcgcttgta atttttactg tattcatatc ataatctcct
     1501 ttcagcttat tatattttta tattacctga ttaaagtatt attgcagtga ctaagtcaca
     1561 aatcggtaat ttccgtttta tgatatgatt ttatccgatt tagatattat tgcagtgact
     1621 aaatcacaaa tcataatata gttttaagtc tattaattta ccaaaatcaa atttattaaa
     1681 ttattttaaa agggtatact ataaaaaaat tattggagat taaaatggaa aaattatttt
     1741 tacagacaga aaacggatta attccccttg acgaaaaaat gattgaaaag tataacttag
     1801 tgaaaggcag aaaatctcct tttactcgtt attatgtagt tgacgaaacg ggaaatcaca
     1861 atttaggttc aacaaaagaa acatcaagtg acgacgttga tagaatgcct gccggggaag
     1921 gtgcagaaga tgatgaaatt gtagaattct ccgacacggg aacaatcctt tcacagtcgg
     1981 aaattattga tttttctcac ggaacagatt cagaggaacc atagtgataa aactattgac
     2041 aattgagcag acattcaact ataatataat caaacggttg aacaatcatt caactgaaaa
     2101 cgggaggtag aaatgtcttc taatgaaggt tttgtatgcg actgtgaagt gattcatgga
     2161 gatattgtta atgaggtcaa aaaaaggatg cccgatgatg aggatattta tgatttatcg
     2221 gatttcttta aggtgttggg tgacagcaca agaatgagaa taatttgggc gctggatgaa
     2281 agcgaaatgt gtgtttgcga tattgctgtt ttgttgaata tgaccaagtc agccatttct
     2341 catcagctgc gtgcattaag aaatgcaaat ttagttaagt tcagaaaaga aggcaagacg
     2401 gtttattact ccttgaagga caagcatata agggatatat acgaaatagg tatggagcat
     2461 atcaaagaaa aatataacaa ataaatcaaa gcaaaatata atgaataaac tgaaccaata
     2521 tgtggaggaa gatatgacaa agacgtataa attgcaagga ttggattgcg ctannnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncagccaa ttaaaattaa taatgtatat
     2701 aataagttat atattagccg gtggcgatgt agtaaaaaga gcaattgaaa acattaaaaa
     2761 cggacaagtt tttgatgaaa attttctgat gtctgctgct tcaataggag cgttttttat
     2821 aggtgaagct cctgaaggtg tcggcgtaat gttattttat cagattggtg aaatgcttca
     2881 aagctatgcg gtgggacaat ccagaagatc tatttcaagt ttgatggata ttcgcccgga
     2941 ttatgcaaat gtattaagga atggcgaagt tcttacaata tctcctgaag aagtggagat
     3001 aggtgaaata atagtaataa aagcgggaga aagggttccc cttgacggta ttgtcgtgga
     3061 agggaactca atgctcgaca catcagctct tacaggcgaa tcacttccaa gggaagtaat
     3121 tacgggaagc gaattgttaa gcggttgcat taatatgaat ggattgctca acgcaaaggt
     3181 tacaaagagc tatagcgaat ccaccgtaaa taagatactt gaattggtag aaaatgcaag
     3241 cagcaggaaa tccaaatcgg agaaattcat aacaagattt gcaagggttt acacgccagt
     3301 tgtggttgta atagcagttg ttctcgcaat tataccgcca ttgattatta agggtgctac
     3361 atttaatgag tggatttaca gatcattggc atttttggtt gtatcatgtc cttgtgcatt
     3421 ggtaatttca gttcctctga gtttcttcgg aggaatagga ggtgcttcca gaaacggtat
     3481 tcttgttaaa ggaagcaatt acctggaatc cttggcgaaa accgaaataa tcgtttttga
     3541 caaaaccgga acattaacta agggtgtttt caaagttcaa aaaattcatt ctgagaaaat
     3601 taaaccggag gagcttttaa aacttgcagc ttatgcagaa agttattcaa atcacccgat
     3661 ttctctttca ttaaaagaag catacaaaaa agatatcgac attgaaaaaa tttctcaagt
     3721 ggaagaaatt tcaggacatg gtgtatcagc agcagtagaa aatataaaag tcttggctgg
     3781 aaatgataag cttatggaaa aattcaaaat tccatacaat aaagaaacat ctgaaggaac
     3841 tctagtccat attgctgcag acggagaata tgcagggtat attctgattg ctgataagat
     3901 aaaggatgat gcttttgaag ctgtaagtga tttaaggaaa cataacatca aggatatagt
     3961 aatgcttacc ggtgacagtg aatcggttgc aataaatact gcaagggaat tgggaattga
     4021 cagggtttat gccgggttat tgcctgcaga caaagttgaa aaagtagaag aattgatttc
     4081 tcaaaaatcg aaaaaggtaa cattaatgtt cgtgggagac ggtattaatg atgcacctgt
     4141 tttggcaaga gccgatgtgg gagttgcaat gggagctctt ggctcagatg ctgcaattga
     4201 agctgctgat gttgtaatta tgacggatga gccgtctaaa attgcaaaag caattgaaat
     4261 tgcgaaaaat actataaaga ttgcatatca aaatgttatt tttgccatat cggtaaaagt
     4321 gataattctg gttttgagca ccttaggcct tgcaagctta tgggctgcag tatttgcaga
     4381 tgttggcgta acctttatag ctgtattgaa tgccatgagg gcacttagac ttaataatta
     4441 aataaatatt taaaaaaatg tgagggtttt agcccgaaga atctcatgaa aaatctcaat
     4501 tacctgttga gattttttgt ttaatatgct aaattatgga tatatataaa agtacaataa
     4561 tataaacaac tcatctatgc gaaacattat acgacagttc tcaagaatcg aaacatct
//
LOCUS       DAOSOW010000104         4616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_18702_length_4616_cov_3.858803, whole genome shotgun sequence.
ACCESSION   DAOSOW010000104 DAOSOW010000000
VERSION     DAOSOW010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18702_length_4616_cov_3.858803"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLD67_03820"
     CDS             complement(<1..186)
                     /locus_tag="PLD67_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT21679.1"
                     /translation="MKFYYDLHIHSDLSPCSACDMTPNNIINMSLIKGLDIISVTDHN
                     STGRSSRRCSSNNSSVSQ"
     gene            complement(183..515)
                     /locus_tag="PLD67_03825"
     CDS             complement(183..515)
                     /locus_tag="PLD67_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOT21680.1"
                     /translation="MNCLEFAEKLQLTPLCEKEGDIEIEGVYIGDLLSIVMAKAKKNY
                     VWITIQTHINIVAIAELLELSCIIVVENMAVDNDTLDKAKELNIPIFKTSESAYQVAC
                     RLSDMGIK"
     gene            complement(528..1769)
                     /locus_tag="PLD67_03830"
     CDS             complement(528..1769)
                     /locus_tag="PLD67_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOT21681.1"
                     /translation="MNFSIMFDKDKCRGCTNCMKRCPTQAIRLIEGKAFINTAKCIHC
                     GECIKICPYNAYSPESIEWYNQMHDTPKKFKIAIPTTSLYGQFPRGTDICKVQNAILK
                     LGFDFVYDASWAADLVAKAIKLKIDKEKTMRPYISTYCPAAVRLIKYRYPSLMDNLIL
                     IREPMEIAGILARERVRKKFGLKDEDIKVFYISPCPAKLLAVTNPIGDYKPSVDWVIP
                     LNKIYGDLYREVIREDNITCSYPSLLGMKWAVAGGQSEHAELNNYIAVHGMENIIEIL
                     EEIENGKLSDIDFVEASACVNGCVGGTFNVVNPFIASSSINYISNSLPDECLNPDIDR
                     FDEMYKTGFFNYKLKEEEKFDKLNLKEAVERNEKIREILDKLPGLDCGSCGAPTCLAH
                     AEDVYNNESEFYDCVVLRAKK"
     gene            complement(1779..2204)
                     /locus_tag="PLD67_03835"
     CDS             complement(1779..2204)
                     /locus_tag="PLD67_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HOT21682.1"
                     /translation="MTDKFLKRYSYNITANDFMSAGKSASAIKSNLKSLGIDSNTIRK
                     ISIISYETEINIVIHSYGGKLHCDIYENKIVIISEDIGPGITDLDMAMTEGYSTATDS
                     VRELGFGAGMGLPNIKKYSDQLEINTSSTGTTIIATIYL"
     gene            complement(2197..2550)
                     /locus_tag="PLD67_03840"
     CDS             complement(2197..2550)
                     /locus_tag="PLD67_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012446581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOT21683.1"
                     /translation="MQIKEIVDIVKADVLTNYHDGNTEFKFGFASDLMSDVLAYGRSD
                     SLLITGLNNPQVIRTVEMLDINAVLFVRGKRPCEEVIELAKENGITILATDYTMFKTC
                     GLLVMNGMEGLEIND"
     gene            complement(2562..3425)
                     /locus_tag="PLD67_03845"
     CDS             complement(2562..3425)
                     /locus_tag="PLD67_03845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOT21684.1"
                     /translation="MEVAILKSFTYENSGGNGAGVVVLNQQIPYDKKQEIANKVGLSE
                     TAFIYKRDENDYDVSFFTPVCEVDLCGHATIAAFYYLGKIGQIKGNDETKCVYQNTKA
                     GRLKIEITFKGQDVEYVLMQQSEPRIYGELKEKKQIADSLNIDERLIGLPNYDIAPTI
                     VSTGLKDILVPVSSRKVLNSLDVDFKMIEDISYENDVVGYHVFTIDNERVYARNFAPR
                     VGINEECATGTSNGALGSLLFSKGFSNQMEVIQGESMNQKSLIFVRIDSHNDIMDVYV
                     GGKAHIENYIK"
     gene            complement(3589..3671)
                     /locus_tag="PLD67_03850"
     tRNA            complement(3589..3671)
                     /locus_tag="PLD67_03850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3636..3638),aa:Leu,seq:gag)
     gene            complement(4105..>4616)
                     /locus_tag="PLD67_03855"
     CDS             complement(4105..>4616)
                     /locus_tag="PLD67_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HOT21685.1"
                     /translation="SSGLKEIIQGTEIAHNFKEIYAAEYCYNENNIPIWPAMAVNYTS
                     KTQFLFRINKGVLDVTEHKGLNESTPDNKKRIPFCNMIYIGDGLTDVPCMKLVKINGG
                     HSIAVYHDDRCIADSLISQGRVDFVVSADYRENSEMENTVRTILNQIAALNKVLKLHA
                     KHMKNLLAD"
BASE COUNT         1353 a          954 c          650 g         1659 t
ORIGIN      
        1 ctgcgagacg gatgagttgt tgctggagca acgccttgaa gacctgcccg ttgaattatg
       61 gtctgttaca gatattatat ccagcccttt tattaaggac atatttataa tattatttgg
      121 agtcatatcg catgcgctgc acggagacaa atccgaatga atatgcaaat cataataaaa
      181 tttcatttta ttcccatatc tgacaatctg catgctactt ggtatgcact ttctgatgtt
      241 ttaaatatag gtatgtttaa ttcctttgct ttatccaaag tatcattgtc gactgccata
      301 ttttcaacaa ctataataca agaaagctcc aaaagctcgg caatagctac aatattaata
      361 tgggtttgaa tagttatcca tacatagttc ttttttgctt ttgccattac aatacttagt
      421 aaatcaccta tatacacacc ttcaatttca atatctcctt ctttttcaca cagtggtgta
      481 agctgcagtt tttctgcaaa ttctaagcag ttcattttcc cctcctctta tttttttgct
      541 ctcagtacaa cacagtcata aaattcagac tcgttattat aaacgtcttc agcgtgagcc
      601 aaacatgtgg gtgctccgca ggagccgcaa tccaatccag gaagtttatc aaggatttcc
      661 cttatttttt cattcctttc aacagcttcc tttaagttaa gtttgtcaaa tttttcttct
      721 tccttcagct tataattaaa gaagccggtt ttatacattt cgtcaaatct gtcgatatca
      781 ggatttaaac attcatcagg cagactgttt gaaatataat taatgctgct gcttgcaata
      841 aacggattta caacattaaa ggttccgcct acacaaccgt taacacaagc tgaagcttcc
      901 acaaaatcta tatcgcttaa tttaccgttt tcgatttcct ctaagatctc aatgatgttt
      961 tccatgccgt gaacggctat ataattattc aattccgcat gttccgactg accgcctgcc
     1021 actgcccatt tcatacctaa tagtgacggg tatgagcatg taatattatc ctctcttata
     1081 acttccctgt acaggtctcc gtatattttg ttaagaggta ttacccaatc taccgaaggt
     1141 ttgtagtctc cgattggatt cgttaccgcc aacaacttgg caggacatgg tgatatataa
     1201 aatactttta tatcttcatc ctttaaaccg aactttttcc tgactctttc ccttgcaagt
     1261 attcctgcaa tttccatagg ctcccttatt aaaataagat tatccatcaa tgaagggtac
     1321 ctgtatttaa taagcctaac cgctgccgga caatatgttg atatataagg tctcattgtt
     1381 ttttctttat ctattttaag ttttatggct tttgcaacca aatctgccgc ccagcttgca
     1441 tcatacacaa aatcaaaacc taatttcaaa attgcatttt ggaccttgca tatatctgtt
     1501 cccctgggga actgcccgta taaagaagtg gtaggaattg caattttgaa ttttttgggt
     1561 gtatcatgca tttgattgta ccactctatg gattcgggag aataagcatt ataagggcat
     1621 atttttatac actcaccgca atgaatgcat tttgccgtat ttataaatgc tttcccttct
     1681 attaatctta tagcctgagt aggacatctt ttcatgcaat tggtacatcc tctgcattta
     1741 tctttgtcaa acataattga aaagttcata ataccctcct acaaatagat tgtcgcaatt
     1801 attgttgtcc cggtactact tgtattgatt tctaattggt cagaatattt tttaatattt
     1861 ggaagtccca tacctgcacc aaatccaagc tctcttacac tgtctgtcgc agtggagtac
     1921 ccctctgtca ttgccatatc taaatccgtt attcccggac ctatatcttc cgaaattatt
     1981 actattttat tttcatatat atcgcagtga agtttcccgc cgtaagaatg tataacaata
     2041 tttatttcag tttcatatga tattattgat atttttctta tagtattcga atcaattccc
     2101 aatgatttta aattgctttt tatggcactt gcacttttgc ctgcagacat aaaatcattt
     2161 gctgttatat tgtagctgta tctttttaaa aatttatcag tcatttattt ctagcccttc
     2221 cattccgttc ataaccaata atccgcatgt tttaaacata gtgtaatcgg ttgcaagaat
     2281 tgtaataccg ttttcctttg ctaattctat tacttcttcg caaggtcttt taccccttac
     2341 aaacaatacg gcattaatat ccagcatttc aacagtccta ataacctgcg gattgtttaa
     2401 ccctgttatc agcaacgaat cgctgcgtcc atatgcaagt acatcactca ttaaatcact
     2461 ggcaaaaccg aatttaaatt cggtattacc gtcatgatag tttgtaagaa catctgcttt
     2521 tacaatatca actatttctt ttatttgcat accatccctc gctatttaat ataattttca
     2581 atgtgggctt ttccgcctac gtatacgtcc ataatatcat tatgagaatc aattcttaca
     2641 aagataagac ttttttggtt cattgattca ccttgaatta cttccatttg atttgaaaac
     2701 cctttactga aaagtaagct tcccaatgct ccgttggatg tacctgttgc acattcttca
     2761 tttataccca ctcttggagc gaaatttctc gcataaactc tttcattgtc aattgtaaaa
     2821 acatgatacc caactacatc attttcataa gaaatatcct ctatcatctt gaaatcaaca
     2881 tcaagactat ttagcacctt cctcgagcta accggtacca aaatgtcttt taaacctgta
     2941 gaaacaattg taggagcaat atcataattc ggcagaccta ttaatctttc atctatattc
     3001 agcgaatctg ctatttgttt tttctcttta agctcaccgt atatacgtgg ttcgctttgc
     3061 tgcataagaa catattcaac atcttgtcct ttaaatgtta tttcaatttt gagtctgcct
     3121 gccttcgtat tttgatacac gcattttgtt tcatcgttac ctttaatttg tccaatttta
     3181 cccaagtaat aaaaagctgc aattgtagca tgtccgcaga ggtctacttc gcaaacggga
     3241 gtgaaaaaac tcacatcata atcgttttca tcccgtttat aaataaacgc cgtctctgat
     3301 aatcctactt tatttgcaat ttcttgcttc ttatcatatg gtatctgttg attcaaaaca
     3361 acaacacctg ctccattgcc accgctgttt tcataggtaa atgattttaa tattgcaact
     3421 tccattttcc cctcaccgct ttattatata atcattataa ttcaaaagca gtataattac
     3481 aagcaaaaat acattatcaa tctttaccct tatcattcta tgaatgaacc gtaacaaaat
     3541 tacacagata aaacaaatag tttcggggat tacccgaaac tatcttagtg cggttggggg
     3601 gatttgaacc cccacgaata tacattcgct agcccctcaa gctagtgcgt ctgccgttcc
     3661 gccacaaccg cattacctat aatattataa tagattgatt aactcttgta aagtaatttt
     3721 tcagtcaatt aagccatcca aaaaatacct gaaacagaaa aattgatttt taatttattt
     3781 atgcattctt ataattagcg ttcatttatt cacatataat atacgggggg attatattga
     3841 ttttaaaaat atgggaattt ctaaaaaaac atatttttat tataattttt atattttgta
     3901 ttttgactat tttattttca cttttacata catgacgagg acgccttccc catgcctata
     3961 aaagggcaag ccgtgaatac attcacggct tgaggcaaaa aatttaagag gcatttataa
     4021 attttcattt acaaatgtat atatattata ctactttttt aaaaaatttc aaatagtttt
     4081 tttattcttc atttatgcat taactcagtc cgctaaaagg ttcttcatat gctttgcatg
     4141 cagttttagc accttattta aagctgctat ttgattaaga atagttcgaa ctgtattttc
     4201 catttcgctg ttttctctat agtcggcact aacgacaaaa tcaaccctgc cttgtgaaat
     4261 caggctgtct gcaatacacc tgtcatcatg atagactgca atggaatgcc cgccgttaat
     4321 tttgactaac ttcatgcacg gcacatcagt caaaccgtca ccaatataaa tcatattaca
     4381 gaaaggtatt cttttcttat tatccggagt ggattcgttt aatcctttat gctctgtaac
     4441 atccaacaca cccttattta ttctgaacaa aaattgagtt ttgctggtat aatttacagc
     4501 cattgccggc catataggaa tattattttc attatagcag tattcggcag cataaatttc
     4561 tttgaaatta tgagcaatct cagttccctg gattatttcc ttcaatcctg aagata
//
LOCUS       DAOSOW010000105         4600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_18799_length_4600_cov_2.768537, whole genome shotgun sequence.
ACCESSION   DAOSOW010000105 DAOSOW010000000
VERSION     DAOSOW010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18799_length_4600_cov_2.768537"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLD67_03860"
     CDS             <1..250
                     /locus_tag="PLD67_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009220770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21686.1"
                     /translation="LNLLVNEEKIVLMATHDPSLALMADKRIVIKNGGIYKVIETGSK
                     EKEVLKELEKMDKIINTLRFKLRSGDTIDTDFGEEMIS"
     gene            481..1377
                     /locus_tag="PLD67_03865"
     CDS             481..1377
                     /locus_tag="PLD67_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT21687.1"
                     /translation="MTKLKADLALLMVTFGWGISYYLTDLSLEDMGPFTLNSNRFLTA
                     FAVAVILAFPKLKNISKRTVKLSFLLGVVLVLVYLGATFGTLYTTLSNVGFLCGLTVI
                     FTPILSSIIYKKLPGKKQTFVILLSFIGIALLTLKDDFSINYDNLLGDLLCIMCAVSY
                     AAHLLITEKAVTFEDVNPFHLGVLQLGTTGVFTLILAFIFETPHLPTQPKYWGPVLFL
                     SIFCTGVAFIVQCVAQQYTEASHVGVIYSLETVFAGIVAFALANDVLSFQSYVGAALM
                     VASIFIMEIDFSKIKIFQKQKQ"
     gene            1587..1859
                     /locus_tag="PLD67_03870"
     CDS             1587..1859
                     /locus_tag="PLD67_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21688.1"
                     /translation="MPINKNVHEQMRNWELQQKQKSQKELREKYEYMNDKYQQMKNSY
                     SGFRNVHKDDNDDAIFDKSSDLADDNSYMRYELNNTFKNKDDKKNK"
     gene            2016..2435
                     /locus_tag="PLD67_03875"
     CDS             2016..2435
                     /locus_tag="PLD67_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOT21689.1"
                     /translation="MKTLKELSDELKKNNIRLTHQRLKVLEYLSNSKNHPTVEQIYNA
                     LKKEVPSLSKTTIYNTLNYLSELNLIQVLAIDENEAHFDAVTNTHGHFKCKTCGKIYD
                     FDIDMDSYRVKELDNFKITEKLVYFKGICERCLSNTN"
     gene            2455..2946
                     /locus_tag="PLD67_03880"
     CDS             2455..2946
                     /locus_tag="PLD67_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOT21690.1"
                     /translation="MENKTLENLMAAFAGESQATRKYEIYSAIAEKEGKHNAAKLFKA
                     AADAEKLHAKKEYELAGKVGKTADNLVDAKAGETYEYQSMYPEFIKVAEAEGNKAAVG
                     AFTFAMKAEEVHAKLYQEALDNLDETEEVFYYLCPVCGNIEKSVPDKCAICGVMGSKF
                     IKY"
     gene            3112..3393
                     /locus_tag="PLD67_03885"
     CDS             3112..3393
                     /locus_tag="PLD67_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008793000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HOT21691.1"
                     /translation="MGFAETFKALSDPIRREILVMLKEGKMSAGEIAEQFDMTAATIS
                     YHLSQLKKAGLLFETKYKNYIYYEINVSVFEELMLWFTQFGGVGNETEG"
     gene            3377..4015
                     /locus_tag="PLD67_03890"
     CDS             3377..4015
                     /locus_tag="PLD67_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007127514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HOT21692.1"
                     /translation="MKLKVDKLLIISTVICLLPIILSLILYDKLPDRMPIHWDIKGNP
                     DNYGSKAFATIGLPLMMAGLNFITHFALNTDPKRANPSVVLKVIGKLTIPFMTVTLVP
                     ITIFAGLGYKIPIEKIVHAFVGVLFIIIGNYLPKSKQNYTVGIKLPWTLNNETNWNKT
                     HRLAGYLWILGGLLMLVNSFLQIYWMPVFIVIMSCVVLIPAVYSYILYKNGY"
     gene            complement(4058..4531)
                     /locus_tag="PLD67_03895"
     CDS             complement(4058..4531)
                     /locus_tag="PLD67_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HOT21693.1"
                     /translation="MCVQKYSSYANMASDPELKSIFQQNGQKEQEHYNTLDQLLKGQM
                     PSMSQSSSQQSSSQQSGGQQSGSQQSGGQMQMKSGGQQSSQSQGNTNYSSSDKDLCTD
                     MLMTEKYVSGAYNTAIFEFRDPQVRSILNHIQKEEQQHGEAIFKYMEKKGMYQMK"
BASE COUNT         1571 a          680 c          837 g         1512 t
ORIGIN      
        1 attaaattta ttggttaatg aagaaaaaat agttcttatg gcaacccatg accctagctt
       61 agctcttatg gcggataaaa gaattgttat taaaaacggc ggcatttata aggttattga
      121 aacaggttca aaagaaaaag aagttttaaa agaattggaa aagatggaca aaataatcaa
      181 cacattaaga tttaagctta gaagcgggga cacaatagat acggactttg gcgaagaaat
      241 gatatcataa aaacgcccac ctgcattcta aaggtgcaac cttgttatta atatgaataa
      301 aggtaagtgt cattctgaga acagtgaaga atctcaaaac atgagatcct ccgttatact
      361 caggacgaca ttttttttgt cattttgagt gctttgctga aggaatccca atttatttca
      421 ttgcctaaat caaaaatatg tattagaata tagcatgaag taaaaaaggg gacagttatt
      481 atgacgaaat taaaagcaga tttggcttta ttgatggtaa cattcggatg gggtatctcg
      541 tattacttaa ccgatttgag tttagaggat atgggaccct tcaccttaaa ttcaaacaga
      601 tttttaacag catttgctgt tgctgttata cttgcctttc caaaattgaa gaatatttca
      661 aaacgtacag ttaaattaag tttccttctg ggcgtcgtat tggtattggt ttatctggga
      721 gcaacattcg gcactttgta taccacattg tccaatgtag gttttttatg cggtcttacc
      781 gtaattttta cccccatatt atcgtcaata atttataaga aactgcccgg caaaaagcaa
      841 acctttgtta ttttgttgag ttttattggc atagcacttt taacattaaa agatgatttc
      901 tctataaact atgacaattt attgggagac cttttatgta ttatgtgtgc tgtgtcttat
      961 gctgcacatt tgttgattac agagaaagca gtaacatttg aagatgtgaa tccctttcat
     1021 ttaggtgttc tgcagcttgg aaccaccggc gtattcactt taattctagc ctttatattt
     1081 gaaactccac atttgccgac acagcccaag tattggggac ctgttttgtt tttatcgatt
     1141 ttctgcactg gagtagcatt tattgttcaa tgcgtagctc aacaatacac agaagcatct
     1201 catgtaggtg taatatactc attggaaact gtttttgccg gaattgtagc atttgcactc
     1261 gccaatgatg ttctttcatt ccagtcctat gtaggtgctg cacttatggt cgcaagtatc
     1321 ttcatcatgg aaattgattt ttcaaaaatt aaaatatttc aaaaacaaaa acaatgaata
     1381 atagtttaca taaataacat tatgtatact tacaaagctt attcattatc aatgtaatag
     1441 acatttgata attttaaacc ggaatctctg cttattgatg agatttacaa ctgtaattat
     1501 cttgcagatt taacttttat acctgtaata aatttaattg gataaacaaa taatatcatt
     1561 aataatataa aagaaaggat gatattatgc caattaataa aaatgttcat gagcaaatgc
     1621 gtaattggga gcttcagcag aagcagaaaa gtcaaaagga attaagagaa aaatatgaat
     1681 atatgaatga taaatatcag cagatgaaga acagttacag cggcttcaga aatgttcata
     1741 aagatgataa tgatgatgca atatttgaca aaagcagtga tttggcagat gataacagtt
     1801 atatgagata tgaattaaac aataccttta aaaacaagga cgataaaaag aataaataat
     1861 tagttttaca ggcaaataag gcaaatattc aatactatga gtatttgcct ttattattaa
     1921 aaattcttta atttttctct tgacatcaaa taaataatac tatatcatta cattgtaatc
     1981 attacaaaat aaaaataatt ataaacgagg tttgaatgaa aacactaaaa gaattatcgg
     2041 atgaattaaa aaagaataat atacgtctta ctcatcaaag gttgaaggta ttggaatatt
     2101 tatcaaacag taaaaatcat cctacggtgg agcaaattta taatgctctt aaaaaggaag
     2161 taccttcttt gtctaaaact acaatttaca atacattaaa ttatttatcg gagttaaatc
     2221 tcatacaggt tttagcaata gatgaaaatg aagctcattt tgatgctgtt accaatactc
     2281 atggccattt taaatgtaag acatgcggta aaatttatga ttttgatatt gacatggatt
     2341 cataccgtgt caaagagctc gataatttta aaatcactga aaaattagtt tattttaagg
     2401 gaatctgcga aagatgcctt tctaatacaa attaaattaa tggaggaatt aaaaatggaa
     2461 aacaaaacac ttgaaaactt aatggcggca tttgcgggag aatcacaagc tactcgaaaa
     2521 tatgaaattt attctgcgat agcagaaaag gaaggaaagc acaatgcggc aaaattattc
     2581 aaagctgctg ccgatgcaga aaaacttcat gctaaaaaag aatacgaatt ggcaggcaag
     2641 gtaggaaaga cagctgacaa cttggtagat gcaaaagccg gagaaacata tgaatatcaa
     2701 agcatgtatc ctgaatttat taaagttgca gaagctgaag gaaataaggc tgctgtagga
     2761 gcatttacct ttgcaatgaa ggctgaagag gttcatgcaa aattatatca ggaagcattg
     2821 gataacttgg atgaaacaga agaagtattc tattatctat gtcctgtttg cggcaacata
     2881 gaaaagtcag ttccggataa atgcgccatc tgcggagtaa tgggctcaaa attcataaag
     2941 tattaaaaat aaatcaacaa aaaatcctct aaaggggatt ttttttattt tgttgggggt
     3001 tattttttgc gggaaaacta tttagaaaaa tttcaaaata gatattgaca ttttaatttt
     3061 tttatagtat attctattta gaaatatttc taaatagaaa gaggtgagac tgtgggattt
     3121 gcagaaacat ttaaggcgct ttctgacccg ataagaagag aaattttggt aatgcttaaa
     3181 gaaggaaaaa tgtctgcggg agaaatagct gaacaatttg atatgactgc tgctacaatt
     3241 tcatatcatc tttcacagct taaaaaggcg ggattgttgt ttgaaaccaa atataaaaat
     3301 tatatttatt atgaaatcaa tgtttcggtt tttgaagaat taatgttgtg gttcacacag
     3361 tttggaggtg ttggaaatga aactgaaggt tgataaactg ttgataatca gtactgttat
     3421 ttgcttgctt cccataattt tatccttgat tttgtatgat aagttacctg atagaatgcc
     3481 tatacattgg gatattaaag ggaatccgga caactacggt tcaaaagctt ttgctacgat
     3541 agggcttccc ttaatgatgg cagggcttaa tttcattaca cattttgcat tgaatactga
     3601 tcctaaaagg gcaaatccat cggttgtttt aaaagttatc ggtaaattaa ccataccttt
     3661 tatgactgta actttggttc ccataaccat atttgccgga ttaggatata aaatacctat
     3721 agaaaaaata gtacatgcat ttgtcggtgt attatttata ataataggta attacctgcc
     3781 taagagcaaa caaaattaca cagttggaat aaagctgcct tggaccctaa acaatgaaac
     3841 aaattggaat aaaacacatc gattggcagg atatttgtgg atattaggcg gattattgat
     3901 gcttgtgaac agctttttgc aaatatactg gatgcccgta ttcatagtta taatgtcatg
     3961 cgtagtttta atacctgcgg tttattcata tattttgtat aaaaacgggt attaaaaata
     4021 gtggtatgaa tgataaaaaa gtggcttagc cactttttta tttcatttga tacattccct
     4081 ttttttccat atacttgaat atagcttccc catgttgctg ctcttccttt tgaatatggt
     4141 ttaggatact tctaacttga gggtctctaa attcaaatat agctgtgtta tatgcaccag
     4201 atacatactt ttcagtcata agcatatcag tgcaaaggtc tttgtcggaa ctgctgtagt
     4261 ttgtgttccc ctgggactgg gatgactgtt gtccgcctga tttcatctgc atctgaccac
     4321 ctgactgctg gcttcccgat tgctgacctc ctgactgttg gctgcttgat tgctgactgc
     4381 ttgattgact catggacggc atctgaccct ttaatagctg gtcaagagtg ttgtaatgtt
     4441 cttgttcttt ttgaccattt tgttgaaaaa tacttttcaa ttccgggtct gatgccatgt
     4501 tggcatagct tgaatacttt tggacacaaa tcttttcatg gtttttttgg tcttcttaaa
     4561 gataagtttc tttagttgta aaattcattc gaacacctcc
//
LOCUS       DAOSOW010000106         4589 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_18871_length_4589_cov_3.740406, whole genome shotgun sequence.
ACCESSION   DAOSOW010000106 DAOSOW010000000
VERSION     DAOSOW010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18871_length_4589_cov_3.740406"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /gene="rpsC"
                     /locus_tag="PLD67_03900"
     CDS             complement(<1..578)
                     /gene="rpsC"
                     /locus_tag="PLD67_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004833616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOT21694.1"
                     /translation="MGQKVNPHGLRVGIINDWDSKWYADKKNFAENLTEDYKIREYIK
                     RENYQAGIAKIEIERFAGRVKVSVFTAKPGMIIGKGGTGVDILKNSIEKITGKTVIIN
                     VEEVKVPELNGQLVAENIASQLEKRISFRRAMKQAMQRTMKVGAKGIKTSVSGRLNGA
                     DMARTEGYSEGKIPLQTLRSDISYGFAEADTT"
     gene            complement(600..935)
                     /gene="rplV"
                     /locus_tag="PLD67_03905"
     CDS             complement(600..935)
                     /gene="rplV"
                     /locus_tag="PLD67_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOT21695.1"
                     /translation="MEAKAVAKYIRISPRKMKFVCDMVRGKQVDEALAILKFHPGKGS
                     RILEKVVKSAAANAENNFDMDRDKLYVVNAYANQGPTLKRWRPRAKGSAYKILKRSSH
                     VGAVVKERE"
     gene            complement(953..1234)
                     /gene="rpsS"
                     /locus_tag="PLD67_03910"
     CDS             complement(953..1234)
                     /gene="rpsS"
                     /locus_tag="PLD67_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOT21696.1"
                     /translation="MGRSLKKGPYCEPSLLKKIVEMNDANTKKVLKTWSRRSTIFPEM
                     VGHTLAIHDGRKHVPVYITEDMVGHKLGEFAPTRTYRGHDKSDKTSKVK"
     gene            complement(1261..2094)
                     /gene="rplB"
                     /locus_tag="PLD67_03915"
     CDS             complement(1261..2094)
                     /gene="rplB"
                     /locus_tag="PLD67_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOT21697.1"
                     /translation="MGIRKYKPTSPALRQMTVPTFEEITTDEPEKSLLTPLKSKAGRN
                     AHGRITVRHKGGGEKRKYRIIDFKRNKDGVPGKVATIEYDPNRSSYIALINYVDGEKR
                     YIIAPNKLQVGDTIVSGEDADIKIGNALPVRNIPVGTMIHNIELKPGKGGQMVRSAGN
                     AAQLMAKEGKYAQVRLPSGEVRMISMDCRATIGQVGNIDHENITIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPVTPWGQPTIGYKTRKKNKKSDKMIVSRRNK
                     K"
     gene            complement(2124..2414)
                     /gene="rplW"
                     /locus_tag="PLD67_03920"
     CDS             complement(2124..2414)
                     /gene="rplW"
                     /locus_tag="PLD67_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOT21698.1"
                     /translation="MRDPHDIIIKPIVTEASMNAMADKKYTFVVDKKSNKTEIKNAVE
                     SIFGVKVASVNTMNMLGKKKRMGVHVGKRPDWKKAIVTLTEDSKTIEFFEGI"
     gene            complement(2414..3037)
                     /gene="rplD"
                     /locus_tag="PLD67_03925"
     CDS             complement(2414..3037)
                     /gene="rplD"
                     /locus_tag="PLD67_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOT21699.1"
                     /translation="MPKVALYDITGSQIGDVELSDDIFGVKVNTHVMYEAVKNYLANQ
                     RQGTQSAKTRGEVRGGGRKPWRQKGTGRARQGSIRAPQWIGGGVVFAPKPRDYSYKLP
                     KKIKRLALKSALSSKVRDNEIIVVDSLVLDKPKTKDMVKILSNLKAGKKTLVVIPERD
                     ENVILASRNIPGVKTAYVNTINVYDILNCDSFLITKDAVNKVEEVYA"
     gene            complement(3064..3696)
                     /gene="rplC"
                     /locus_tag="PLD67_03930"
     CDS             complement(3064..3696)
                     /gene="rplC"
                     /locus_tag="PLD67_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOT21700.1"
                     /translation="MKAILGKKIGMTQVFNEQGEVIPVTVLESEGMVVVQKKTEEKDG
                     YNAIQVGFGDVKIKNVIKPKKGHFDKSKVEPKRFLREFRVDNIDDYEVGQKIGVDIFQ
                     AGEKVDVTGISKGKGFQGSIKRHGHARGDMSHGSKFHRLRGSLGASAGVGKVVKGTKA
                     HGHMGNEKVTVLNLEVVKVDADKNVILLKGAVPGPKKGLVKIREAVRKSK"
     gene            complement(3828..4139)
                     /gene="rpsJ"
                     /locus_tag="PLD67_03935"
     CDS             complement(3828..4139)
                     /gene="rpsJ"
                     /locus_tag="PLD67_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOT21701.1"
                     /translation="MANTQKIRIRLKAYDHQLIDQSAQKIVETAKSTGATVAGPIPLP
                     TEKQIITILRAVHKYKDSREQFEQRTHKRLIDITNPTPKTVDSLMKLNLPAGVDIEIK
                     L"
     gene            complement(4484..>4589)
                     /locus_tag="PLD67_03940"
     CDS             complement(4484..>4589)
                     /locus_tag="PLD67_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21702.1"
                     /translation="VELIHPIAIEEGLRFAIREGGRTVGSGVVTEIIK"
BASE COUNT         1065 a         1079 c          666 g         1779 t
ORIGIN      
        1 aacgttgtat ctgcttctgc aaaaccgtag cttatgtcag accttaaagt ttgaagagga
       61 attttacctt ctgaatatcc ttcggttcta gccatatctg ccccgttcaa tcttccggat
      121 acacttgttt taattccctt tgccccaact ttcatagttc tttgcattgc ctgtttcatt
      181 gcccttctga atgaaatacg cttctcaagc tgacttgcaa tgttctctgc aactaattgc
      241 ccgtttaact caggaacctt aacttcttcc acattgatta ttacagtctt acctgttatt
      301 ttctctatgc tgttctttaa aatatcaaca ccggtaccgc cttttccgat aatcataccc
      361 ggcttagcag taaatacact tactttaact ctgccggcaa atctctcaat ttctattttt
      421 gctatacctg cttgatagtt ttctcttttt atatattcac gaattttata gtcttcagtt
      481 aaattttctg cgaaattctt tttgtcggca taccattttg aatcccaatc attgattata
      541 ccaactctta gtccatgagg gtttaccttt tgacccatct tttacctcct ttactgtagt
      601 tattctcttt ctttaacgac tgcaccaacg tgactgcttc tttttaatat tttatatgcc
      661 gaaccctttg ctcttggtct ccatcttttg agagttggtc cttgatttgc atatgcattt
      721 acaacatata atttatctct atccatatca aagttgttct ctgcattagc tgccgcagat
      781 ttcactactt tttctaaaat tcttgagccc ttgccgggat ggaattttaa aattgccaat
      841 gcttcgtcaa cttgctttcc tcttaccata tcacacacga atttcatctt tcttggtgat
      901 attctgatat atttagcaac tgccttagct tccatcagcg aatcctccct tcttatttaa
      961 ctttcgaagt tttgtcggac ttatcatgtc ctctatatgt tcttgtcgga gcaaattcac
     1021 ccagcttgtg tccgaccata tcttcagtta tatatacagg aacgtgcttt ctgccatcat
     1081 gtatagccaa tgtatgacct accatttcgg ggaatattgt cgatcttctt gaccaggtct
     1141 tcaacacttt tttagtattt gcatcgttca tttccacaat ctttttcaaa agacttggct
     1201 cacaataagg tcctttcttt aacgatctac ccattcacta tttcctcctt tcaatcctgc
     1261 ttatttctta ttccttctgc ttacaatcat tttatcggat ttcttatttt tcttacgagt
     1321 tttataaccg attgtcggtt gtccccaagg tgtaaccggt gacggtcttc ctattggagc
     1381 tcttccttca ccaccgccgt gagggtggtc gttaggattc attacagagc ctctaaccgt
     1441 aggtctgatt cccatatgtc tctttcttcc ggccttgcct attgttatat tctcgtggtc
     1501 aatatttcct acctgaccga ttgttgctct gcaatccatg cttatcattc gaacttcacc
     1561 gcttggaagt cttacctgtg catattttcc ttccttagcc ataagctgag ctgcatttcc
     1621 tgccgagcga accatttgtc cgccttttcc gggttttaat tctatattgt gaatcattgt
     1681 accaaccgga atatttctta cagggagagc attgccgatt ttaatatctg catcctcacc
     1741 tgaaacgatt gtatctccaa cctgtagttt gtttggagct ataatatatc ttttttctcc
     1801 atcaacataa tttataagtg caatgtatga gcttctgttt ggatcatact ctattgttgc
     1861 aacttttccc ggaactccat ctttatttct tttgaaatct ataattctat attttctctt
     1921 ttcaccgccg cctttgtgac gtaccgtaat tcttccgtgt gcatttcttc ctgctttgct
     1981 tttcaacgga gttaaaagag atttctcagg ttcatcagtt gttatctctt caaatgttgg
     2041 aacagtcatt tgtctcaacg ccggagaagt tggtttatat tttctaatac ccatgagatt
     2101 ttcctccttc tttaaatttt agcttatatc ccttcaaaga attctattgt cttgctgtct
     2161 tctgttaaag taacaatggc ttttttccag tcgggtctct tgcccacatg tactcccatt
     2221 ctcttctttt taccaagcat gttcatcgtg ttcacacttg ctactttaac accgaaaatt
     2281 gactccactg catttttgat ttcagtcttg ttagacttct tatcaacaac aaaagtgtat
     2341 ttcttgtctg ccatagcatt catacttgct tctgttacta tcggttttat gataatgtcg
     2401 tgtggatcgc gcattatgcg tacacctcct ccactttatt aactgcatcc ttagttataa
     2461 ggaaagaatc acagtttaat atatcgtaaa cattaattgt atttacatat gccgttttta
     2521 cgcccggaat atttcttgat gctaatatta cattttcatc tctttcagga ataaccacca
     2581 atgttttctt gcctgcttta agatttgata atatttttac catatccttt gtttttggct
     2641 tatcaagaac caaactatcc actacaataa tttcattatc ccgtactttt gagcttaatg
     2701 ctgatttaag cgctaatctt tttatctttt ttggaagctt atagctgtaa tctctcggtt
     2761 ttggagcaaa tacaacacct ccgcctatcc attgcggagc ccttatactt ccttgtcttg
     2821 cacggcctgt tcccttttgc ctccaaggct ttctgccgcc gcctctcact tctcctcttg
     2881 tcttagcaga ctgagttcct tgtctttgat tagccaaata attttttact gcttcataca
     2941 ttacgtgagt gtttactttt actccaaata tgtcatcact taactctacg tcacctattt
     3001 ggctgcctgt tatatcataa agagctactt ttggcatctt taatcctcct ttccacgcaa
     3061 ttcttactta gatttcctaa ctgcttctct gattttcact aatccttttt taggtcccgg
     3121 tacggcacct tttaaaagta ttacattttt atcagcatct acctttacca cttcaaggtt
     3181 taatactgta actttttcat ttcccatgtg gccgtgagct ttcgttccct taactacctt
     3241 tcccacgcct gccgaagctc ccaatgaacc tctcaatctg tggaatttgg agccgtggct
     3301 catgtcgccc cttgcatgtc cgtgtctctt tatcgatccc tggaatccct taccttttga
     3361 aattcctgta acgtcaactt tttctcctgc ttggaaaata tcaacgccaa tcttttgacc
     3421 aacttcataa tcgtcaatgt tatctactct gaattctctt aaaaatcttt taggttctac
     3481 ttttgattta tcaaaatgac ctttcttagg tttaataaca tttttaattt taacgtctcc
     3541 gaatcctacc tgtatggcat tgtatccgtc tttttcttcc gttttctttt gaacgacaac
     3601 catgccttca gattccaaaa cggtaacagg aataacttct ccttgttcat tgaacacttg
     3661 agtcattccg attttctttc ctaatattgc tttcatttta cacctcctaa tttcttacag
     3721 cggattgctt gcgcaatcat tctaagcaga gtgtgttgtc tctacttttt aaacatcgtc
     3781 agaaattgcc atgcaattcc atcacaccgt ttcaacgatg tatatcctta taatttgatt
     3841 tcaatgtcta cccctgcagg caaattcaac ttcataagcg aatcaacagt ctttggagta
     3901 ggatttgtta tgtcgatcaa tcttttgtgt gttctttgct caaactgctc tctagaatct
     3961 ttatacttgt gaactgctct aagtattgtt attatttgtt tttccgtggg cagcggaata
     4021 ggacctgcaa ctgttgctcc ggtactttta gctgtttcca cgattttttg cgcagattga
     4081 tcaattaatt gatgatcata agcctttaac ctgattctta ttttttgtgt gtttgccatt
     4141 aaattacctc cttttcgtat gtctccatac aactcgggac cgctcgataa acccatccgg
     4201 gtgtttcata ttttttttta taaatagcgg taccggtcgc cctgctttta gcaggacata
     4261 ctctgtaaaa attccctgaa acgtttatat ttcagcaacc ttttacgtca tcgcatgtgg
     4321 caagcttttt aataatatac tatcaaatgc tataaatcaa gtgttttttt tcgatttttt
     4381 aagttatttc caaaacttaa tatatattaa tttcccttaa taaatgacaa ggggacgggg
     4441 gtattgtcat cccgtcccct tgttataaaa gcatctaggc ttcttatttg attatctcgg
     4501 ttactactcc ggagcctact gttcttccgc cttctctgat agcaaatctc aaaccttctt
     4561 caatagctat cggatggatt aactcaact
//
LOCUS       DAOSOW010000107         4587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_18881_length_4587_cov_4.188879, whole genome shotgun sequence.
ACCESSION   DAOSOW010000107 DAOSOW010000000
VERSION     DAOSOW010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18881_length_4587_cov_4.188879"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..>4587
                     /locus_tag="PLD67_03945"
     CDS             212..>4587
                     /locus_tag="PLD67_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidyltransferase"
                     /protein_id="HOT21703.1"
                     /translation="MNRSVLKELQKEILLNLTQNEFLKSLGISENKIQSYLNNSKFTK
                     GLSTLFNKNQITCKAVLDLSADILNNEQVEPPENWLSYIFQYVLEKSFPDAVTIELNS
                     EYEISAVIYLEILRTILKYAQINNIGKVPKFEFLTEEEINDLSNKQEYLNFLDVFDKN
                     YIYELMMLDGEVNGYNTLHHVSTVHFVAMHVAKQLKKAGVQVDLGIVSGSAAGHDIGK
                     YGCKGLEKRRVAYLHYYYTDQWFKKYDMPGIALIAANHSTWDLELENLSLESLLLIYS
                     DFRVRNKITKTGEEMQIFSLAESFDIILKKLDNVDEAKEKRYIRVYSKLRDFEDYLLN
                     KGINTDLLSEEPKYVKTVDFALIDGYEVVKNFKFKAFEHNIPLMRMLNNEVTFTGMIE
                     AARSEWDWKNIRAYLNILEEYSTYLSQKEKLFALSFLYELLVHREGDIRRQAAKLMGT
                     IIIHYDMGYTKEMPEDVKITHKEKNAGLSLWDKYLGFFLTPGYKVTDKQKEWIGYSLR
                     MFVESVINSPRNTLKEEYLEIFLKHIREDINDETARFNSLNSLLSIPLELYDKEQLDF
                     VVDFSIKHFRDTSYSIRLMAAQFLFKAVQQIKITGHTLKEILNIVSEFSPDDGLCMNY
                     LKYKTAQCLNVPGTLLKKYSSLLAGNWYKTSDIFLNNLKAATPWNVKTVSIDYIMENL
                     SQRNELALLQTATHLANLVKVSAMESVRNKAGNSLVQLGPMLTIDQRNEIAFELIKGL
                     EIDEMQYAKYIPEYLGRFVMLLSPKELDEFIIDLKNIYINSSERSSALVIHTFGIMVQ
                     YYPEYKERFGEDSSVIEKRLIKILGIILGGLANFNTQVKQETFLVIGQYIFGSKILTL
                     KQKHKVFSLIYKKLLTLISEKELSELFFFNNSASFNHIYRFISDYEFFNGKFDIKENK
                     NIAFFPGTFDPFSLSHKGIVKEIRNLGYDVYLAVDEFSWSKKVQPRLIRRQIINMSIA
                     DELGVFLFPDDVPVNLSNNKDLKILKTLFPKKDIYIVVGSDVLINATAYNNEPEEDSI
                     HNFNHIVFKRAKDEITDEAVKKAEEAKKRIIGTLVELKLPVYLEDISSTQIRENIDNN
                     RDISNLIDPMAQNFIYDRNLYIREPLNKAVLRTKPFVIEIVKELSKKIVDEIDHCIFN
                     DTRLFENIAEKLNFKNIRLLVIRDSKNYNEMLGFSAFHKISTSDVYSEFKSPNIANYV
                     REITSGRIIVIDGIFAAPGRIYDSMEQTLITETLSHCIKNDFTYVLYNNIITGFDSDE
                     LLETLKLQGFAKIHDKSTGKIVYGVDMKFPICLTFNLESFIKEPLNENKNVYEAISYS
                     RKRLQRAMTQLYPGSLVLSFDNDMINQILINKICSLNNVPNEMQEPRVLGEYMVVPFG
                     NVLKGMIVPNTVTKSLHTEKVYSSDATKFKIKEYPFYSSIENQIRTIKSFEKPVILVD
                     DLLHKGYRIKEIDPILKRYNINV"
BASE COUNT         1648 a          696 c          872 g         1371 t
ORIGIN      
        1 aataagaata ctttttcaag ataaaagtgg atgtccagcg gggacggttc tcggtggaca
       61 aaggatgaca ctacccccgt ccccttgtca taaaggatga cactaccccc gtccccttgt
      121 cataggaatg tccccgttag tacaactttc aattgcagta ccaatgagag tatgttataa
      181 tttcgtaaat taaattaaaa gggaggttac aatgaacagg tctgtactta aagaattaca
      241 aaaagaaatt ttacttaatc ttactcaaaa tgaatttttg aaaagtctgg gtatttccga
      301 aaataaaata caatcttatc tcaataattc aaaattcact aaaggcttat caaccttatt
      361 caataaaaac caaataacct gtaaagctgt acttgacctg tcagcagata ttttaaacaa
      421 tgagcaagtt gaacctcctg agaactggtt atcatatata tttcaatatg ttttggaaaa
      481 gtcctttccg gatgccgtta caatagagct gaattccgaa tatgaaattt cagctgtcat
      541 atatcttgaa atattgagaa ctattttaaa atatgcgcaa ataaacaata tcggtaaagt
      601 tccgaaattt gaatttttaa ctgaagaaga aataaacgac ctttcaaaca agcaggaata
      661 tttgaatttt ttggatgtgt ttgataagaa ttatatttat gaacttatga tgcttgacgg
      721 ggaggtcaac ggttacaata ctttgcatca tgtttcgaca gtacattttg ttgcaatgca
      781 cgttgcaaag cagcttaaga aggccggagt ccaggtggac ttaggcattg tttccggctc
      841 tgccgcaggc cacgacattg gaaagtacgg ttgtaaggga ttagaaaaaa gaagggttgc
      901 atatcttcac tattattata ccgaccagtg gtttaaaaag tacgatatgc cggggattgc
      961 tttaattgca gcaaatcatt ccacttggga tttggagctt gaaaacttat ccctggaatc
     1021 attattgtta atatattctg attttagagt aaggaacaaa ataaccaaga ccggtgaaga
     1081 aatgcaaatt ttttcattgg cggaatcatt tgacatcatt cttaaaaagc ttgacaatgt
     1141 agatgaagca aaagaaaaaa gatatataag agtatattcc aaactaaggg attttgaaga
     1201 ctatttatta aacaaaggaa taaatactga ccttttatcg gaagagccta agtatgtaaa
     1261 gacagttgac tttgccctaa tagacggata tgaggtggtg aaaaatttta aatttaaggc
     1321 gtttgagcac aacattccct taatgagaat gcttaacaat gaagttacat ttacaggaat
     1381 gattgaggct gccagaagcg agtgggattg gaaaaatatc cgtgcatatt taaatattct
     1441 ggaggaatac tccacatatt tgtctcagaa ggaaaagctg tttgctttaa gtttcctgta
     1501 tgaactattg gttcacaggg aaggagatat aaggcgtcaa gcagcaaaat tgatgggaac
     1561 aattattatt cattatgata tggggtatac aaaggaaatg cctgaagatg tgaaaataac
     1621 acacaaggag aaaaatgcgg gtttatcctt gtgggataag tatttgggat ttttccttac
     1681 tcctggatat aaagtaacgg acaaacaaaa agaatggatt ggttattctt taagaatgtt
     1741 tgttgaatca gtgataaact ctccaagaaa cacattaaag gaagaatacc tggagatatt
     1801 tttaaaacat atccgtgaag atataaatga cgaaaccgca aggtttaaca gtttaaactc
     1861 tcttttaagc attcctttgg agttatatga taaagagcag cttgactttg tggtggattt
     1921 ttcgataaag cattttagag atacatcata cagcataagg ctgatggcag cacaattttt
     1981 attcaaggct gtgcaacaga taaagattac gggacatact cttaaggaaa tactcaatat
     2041 cgtatcagag ttttctcccg atgacgggct gtgcatgaat tatttgaaat acaagacagc
     2101 gcaatgttta aatgtacccg gtacattact taaaaaatat tcatctcttt tagcaggcaa
     2161 ttggtataaa acatcggata tatttttaaa taatttgaaa gctgcaacac cctggaatgt
     2221 aaaaaccgta agtattgatt acataatgga gaatctttcc caaagaaacg aacttgcgct
     2281 tctccagacg gcaacacacc ttgcaaatct tgtaaaggtt agtgccatgg aatcagtgag
     2341 aaacaaggcc ggtaactctc ttgtacaatt aggtcccatg cttaccatag accaaagaaa
     2401 tgaaatagca tttgagctta ttaaaggtct tgaaatagac gaaatgcagt atgcaaagta
     2461 tattcccgaa tatttggggc gctttgtaat gttgttgtct ccaaaagagt tggatgagtt
     2521 tattattgat ttaaaaaata tttacattaa ttcaagcgag cgctccagtg cattggtaat
     2581 acacactttc ggaataatgg ttcaatatta ccccgaatat aaggaaaggt ttggagaaga
     2641 cagcagcgtt atagaaaaaa gactcatcaa aattttaggg ataattttgg gagggcttgc
     2701 taattttaat acacaggtaa aacaggaaac atttttggta atagggcagt atattttcgg
     2761 ctctaaaata ttgactctta agcagaagca taaggtgttt tcacttattt ataagaagct
     2821 tttaacatta ataagcgaga aggaattaag cgagctgttc tttttcaata actccgcctc
     2881 atttaaccac atatacaggt ttataagcga ttatgagttt ttcaacggta aattcgatat
     2941 aaaagaaaat aaaaatattg cgtttttccc cggaaccttt gacccctttt cgctgagtca
     3001 caagggaatc gtaaaggaaa taagaaattt aggatatgac gtatacttgg ctgtagatga
     3061 attttcctgg tctaagaagg ttcagcccag attgataaga agacaaatta tcaatatgtc
     3121 aattgcagat gaattaggtg tatttttgtt tcctgatgat gtacccgtaa atttatcaaa
     3181 caataaagat ttaaaaatac taaaaacact ttttcccaaa aaggatattt atatcgttgt
     3241 aggaagcgat gtattaataa atgcgacagc atataataat gaacccgaag aagattcaat
     3301 acataatttt aatcacatcg tatttaagag agcaaaggat gaaattaccg atgaagccgt
     3361 taaaaaggca gaggaagcaa agaaaagaat aataggaaca cttgttgaat taaagcttcc
     3421 tgtttattta gaggatataa gctcaaccca aataagggaa aatatagaca ataacagaga
     3481 tatatcaaac ttaatcgacc ccatggctca aaatttcata tatgacagga atttgtacat
     3541 cagggagcct ttaaacaagg cagtactgag aacaaagccc tttgttattg agatagttaa
     3601 agagttgagc aaaaaaattg ttgatgaaat tgaccattgt attttcaatg atacaagact
     3661 gtttgaaaat attgcagaaa agcttaattt caaaaatatt agactgcttg taataaggga
     3721 ttccaaaaat tataacgaga tgcttggatt ttctgcattt cataaaataa gcacctccga
     3781 tgtttattcc gagtttaaaa gtcccaatat tgcaaattat gtgagagaaa taacatccgg
     3841 aagaattatt gttatcgacg gaatatttgc ggctcccggc aggatatatg acagtatgga
     3901 gcaaaccctt attacggaaa ccttatctca ctgcataaaa aatgatttta cctatgtctt
     3961 atataataat attattaccg gctttgacag cgatgaattg ttggaaacat taaaacttca
     4021 aggatttgcc aagattcatg acaaaagcac cggaaagatt gtttacggag tagatatgaa
     4081 gtttcccata tgcttgacat ttaatctgga aagttttatt aaggagccct tgaatgaaaa
     4141 taaaaatgtt tatgaagcta tttcttattc gagaaaaagg cttcaaagag caatgacaca
     4201 gctgtatccc ggaagtcttg tattgtcatt tgacaatgat atgataaatc aaattcttat
     4261 taataaaata tgcagtttaa acaatgtgcc caatgaaatg caagaaccga gagtattggg
     4321 cgaatacatg gttgtgcctt tcggcaatgt tttaaagggt atgattgttc caaacacagt
     4381 aactaaatca ttgcatacgg aaaaagtata ttcttccgat gcaactaaat tcaaaattaa
     4441 ggagtatccg ttttactcat ctatagaaaa tcagataaga accataaagt cttttgagaa
     4501 accggtaatt ttggtcgatg atttacttca taagggttac aggattaaag aaatagaccc
     4561 tatattgaag cgttataata taaatgt
//
LOCUS       DAOSOW010000108         4584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_18898_length_4584_cov_4.039523, whole genome shotgun sequence.
ACCESSION   DAOSOW010000108 DAOSOW010000000
VERSION     DAOSOW010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4584
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18898_length_4584_cov_4.039523"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLD67_03950"
     CDS             complement(<1..329)
                     /locus_tag="PLD67_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HOT21704.1"
                     /translation="MDKVIITGNDLTLDEVVAVCRNYKKVELSDFAVERILKSRKVVD
                     DFVENEDVVYGITTGFGKFSDVTISKEESKTLQKNLIISHSVGAGKPFDSEVVRGIIL
                     LRINNLA"
     gene            complement(461..1021)
                     /locus_tag="PLD67_03955"
     CDS             complement(461..1021)
                     /locus_tag="PLD67_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21705.1"
                     /translation="MKNKIILILICFLLFITGCAVNDNPPEKETEPYGKYTANGRIVK
                     IDKDGFHVEGEDNVGLYKVDMEKNKNFYIGERVRLNSMDGNSYDVELDEEYDYTAAMT
                     AEIFDDDSKLDLKVGEISRDEMGRMRIYGLAADDKEYDIIVGADTITNFAHSTLKVGD
                     NIYVYPKDVPNGIPAVVDAKAVTVVR"
     gene            1296..1730
                     /locus_tag="PLD67_03960"
     CDS             1296..1730
                     /locus_tag="PLD67_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOT21706.1"
                     /translation="MDFLDMQIIKHLEKEGRISHEELSKRLNLSRPAIHNRVAKLEDQ
                     GIITGYKAMIDWSKLGFTTYAIISLRVRTADYDKLLEQLKNLAIPDLVIEECHIVTGT
                     WCILMKVRCSAPSGLKAIQDELHKIECIRESSTSLILSSLYD"
     gene            complement(1767..2210)
                     /locus_tag="PLD67_03965"
     CDS             complement(1767..2210)
                     /locus_tag="PLD67_03965"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOT21707.1"
                     /translation="MKKFKKFTIIFCALMLLLVACAPGDGNLNEDEDNEPIDEPIDDN
                     NTGANANNDTFDEEEKTLTGSAQGYGGEINVTVVVNGEDIVSVEAEGKDETEGVGSLA
                     IDELPDKIAQANSTDVDNVSGATKTSEGIKQAVNNALQSNKQSKQ"
     gene            complement(2337..3710)
                     /locus_tag="PLD67_03970"
     CDS             complement(2337..3710)
                     /locus_tag="PLD67_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HOT21708.1"
                     /translation="MILSEEQKNHYKRQIIIPEIGEQGQKKLLDSTVLVYCENTDSLR
                     PLAYYLAAMGIGKIFCDLQDKIGADSLFAEVIDLNNDTKIDYLNEENYSGGFSEIYEN
                     NKNHPQNFCRIVLGNYSFIKNSAAKLLNDKYLPTIISANSQWKGTLQTFINEDLFKNF
                     IALIADNNTKDFSPPFLANALSATLSSIECVKLCLNIGNITKDMLFFDLFNMEFKQTD
                     NNANLFEFLNEANKMDIKQKLSDAKVLIVGVGGLGSPAAYGMAMADVNTLGLLDLDEV
                     EMSNLNRQILHSFSRIGMPKASSAEFMLKKLNPSINIKTYKTELTRDNAEKIFLEYDL
                     IIAAVDNIPSRYLINDICFLLNKPFAEAGVLRFDGTATTLVPKEGHCYRCLYPNVDSS
                     NLSGNNGVLGSVPGVMGFIQAAEAVKILSGFGKTLKNKILLFDSLAMDFNIIDIEKNP
                     RCPTCGK"
     gene            complement(3741..>4584)
                     /locus_tag="PLD67_03975"
     CDS             complement(3741..>4584)
                     /locus_tag="PLD67_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT21709.1"
                     /translation="GYDKVQQIKMLYGRMINSKDIERKSKVVVLEQKAALKLFGKDNA
                     VGKTIRVKIDNNMEDLLVVGVYKIEQSPLLTLMQGQSNYENSFIPYTMPTSSYYAYYA
                     LDIFIDEKYSMAITGDEIINYIARMKNRTPENYIYYTVEEEQTQVESVVTGLSVAVAA
                     IAAISLLVGGIGIMNIMLVSVTERTREIGIKKALGARTKDVLFQFLIESATLSAIGGI
                     VGTITGIGLVMIGGSFISIPVVVNPASIILAVVFAMVIGIFFGYYPAKKAANSDPIEA
                     LRYE"
BASE COUNT         1381 a          897 c          653 g         1653 t
ORIGIN      
        1 tttgccagat tgtttattct caacagtatt attcctctga caacctcact gtcaaacggt
       61 tttcctgcac ctaccgagtg agatattatt aaatttttct ggagtgtttt tgattcttct
      121 tttgatatgg ttacatcgct gaactttccg aagcctgttg taataccgta gactacatct
      181 tcattttcaa caaagtcatc aacaactttt cttgatttca aaatcctttc aacagcaaaa
      241 tctgataatt ctaccttttt ataatttctg cacactgcta ctacttcgtc caatgtcaaa
      301 tcatttcctg taattataac cttatccatt tttcctccct taaaaaaagg taaatttcag
      361 cataataaaa cgctaaaact tacctcctaa catccatttt cttgaagtat ttttattcta
      421 taacacatta tttattttgt caatcagtgc agaaattata ttaacgtacc actgtaacag
      481 cttttgcgtc aactactgca ggtataccgt tcggtacatc cttaggatat acatatatat
      541 tatcgcctac ttttaatgtt gaatgagcaa aatttgttat tgtatcggcg cccactatta
      601 tatcatattc cttatcgtca gctgccagtc cgtaaatcct cattcttccc atttcatctc
      661 tcgatatctc gccaacttta agatccaatt tagaatcatc atcaaaaatc tctgcagtca
      721 ttgcggcagt gtaatcatat tcttcgtcta attcaacatc ataactgttt ccatccatag
      781 aatttaatct tacgcgttca cctatgtaga aatttttgtt tttctccata tcaactttgt
      841 aaagtcccac attatcttca ccttcaacat gaaatccgtc tttatctatt ttaacaattc
      901 ttccattggc agtatattta ccatatggct cggtctcttt ttccggtgga ttatcattta
      961 cggcacatcc tgttatgaac agcaaaaaac aaattaatat taatattatt ttatttttca
     1021 taaatacctc caatttaaca tttatgtatt atttatattt gccaaaataa tactatatat
     1081 tcatgcaata ttaattgtag cgataatact ttttatagtt taaagattca taaaggctta
     1141 aatcatgaat tcccggctcg cttcgaattc taaattcata tgcattattt aaaaatttat
     1201 taaataatta tttacatgtt gtaccttttt gtgctatatt ataactgatt agttttttat
     1261 tcttcaaata atttacgata tgacaagagg tagatatgga tttcttagac atgcaaataa
     1321 ttaaacactt ggaaaaggaa gggagaattt cacacgaaga gctttcaaaa agattaaatt
     1381 tatcaagacc tgcaatacat aacagagttg caaaattgga agatcagggt attataacag
     1441 gatacaaggc aatgatagac tggtcaaaac ttggttttac cacatatgca attatttcat
     1501 taagagtgag aactgctgat tatgataagc ttttggaaca attaaaaaac ttggctattc
     1561 ctgatttagt aattgaagaa tgccatatag tcaccggcac ttggtgcata cttatgaaag
     1621 taagatgcag tgcaccttca ggtttaaaag ctattcaaga cgagctgcat aaaatcgaat
     1681 gtatcaggga atcatccacg tcattgattt tatcctcctt atatgattaa aaccagcctg
     1741 tcggctggtt tttcatatgc attttgctat tgttttgatt gtttattact ctgtaatgca
     1801 ttgttaaccg cttgctttat tccttcgctt gttttcgtag caccagatac attgtcaaca
     1861 tctgttgaat ttgcttgtgc tattttatca ggaagttcat ctattgccaa agagcctaca
     1921 ccctcagttt catcttttcc ttcagcttca accgatacta tatcctctcc gttaactaca
     1981 actgttacgt ttatttctcc tccatatcct tgtgcagatc ctgtcaatgt tttttcttct
     2041 tcgtcaaagg tatcattatt tgcatttgct ccggtattat tgtcatcaat aggttcatca
     2101 ataggctcgt tgtcttcgtc ctcatttagg tttccatctc caggtgcaca tgcaaccaat
     2161 aaaagcatta atgcacaaaa aataattgta aattttttaa attttttcat agtcacctcc
     2221 ataatgaatt attttttaat agtatctcca gaaataactc aatataaaga ggtaaaaatt
     2281 taaatattta gaaatttttc agttaaaaaa tttcttacat aaatgagaat aataggttat
     2341 tttccacatg taggacatcg gggatttttt tcaatatcaa taatattaaa atccattgcc
     2401 aatgaatcaa acaagagtat tttatttttt aaggtttttc caaatccgga caatattttt
     2461 actgcttctg ccgcttgtat aaaacccata actcccggaa cagaacctaa gacaccatta
     2521 ttacctgata aattgctgct gtccacattg ggatataaac acctgtaaca atgtccttcc
     2581 ttaggaacaa gggtggttgc agtgccgtca aatctcaaaa ctcctgcctc agcaaaaggt
     2641 ttatttaata aaaagcatat atcatttatc agataccttg aaggtatatt atcaaccgca
     2701 gcaataatca ggtcgtattc caaaaaaatt ttttctgcat tatctcgggt taattcagtt
     2761 ttataggttt taatatttat gcttgggttt agctttttta gcataaattc tgctgagctt
     2821 gccttcggca taccgatgcg tgaaaaggaa tgaagtattt gtctgtttaa attgctcatt
     2881 tccacttcat ctaaatccaa taaccccaat gtgttgacat cagccattgc cattccgtag
     2941 gctgccggag agcctaagcc tcccacacct acaatcaaga cttttgcatc agataatttt
     3001 tgtttaatat ccattttatt tgcttcattt aaaaattcaa acaagttagc attattatct
     3061 gtttgtttaa attccatatt aaacaaatca aaaaacagca tatcttttgt tatattaccg
     3121 atattaaggc aaagcttaac acattctata gaagaaagcg ttgcagataa tgcattggct
     3181 aaaaaaggcg gactaaaatc ttttgtatta ttatctgcta ttaatgcaat aaaattctta
     3241 aacaaatcct cattgataaa tgtctgcagt gtacccttcc attggctgtt agctgatata
     3301 atagtcggta aatatttatc gtttaataat ttagctgctg aattttttat aaaactataa
     3361 tttcccaaaa caatcctgca aaaattttgg ggatggttct tgttattttc atatatctcc
     3421 gaaaaacctc ccgaatagtt ttcttcatta agataatcaa tttttgtatc attgtttaaa
     3481 tcaattacct cagcaaataa ggagtcagcg ccgattttat cttgtaaatc acaaaatatt
     3541 tttcctattc ccatagcagc aagatagtat gccaagggtc ttagactgtc tgtattttcg
     3601 caatacacca agacagttga atcaagaagc tttttttgac cctgttcacc tatttcaggt
     3661 attattattt gtcttttata atgatttttt tgttcttccg acaatatcac aatatcactc
     3721 cttttttgta ttttttgtta ttattcgtat ctcaaagctt cgatggggtc tgaattggct
     3781 gccttcttgg ctggataata gccgaagaat atcccgatta ccatggcaaa tacaacagcc
     3841 aaaatgatag aagccggatt tacaaccacg ggtatggata taaaagaacc ccctatcata
     3901 actaaaccta tgcccgttat ggttcccact atccccccga ttgctgaaag tgtggctgat
     3961 tctatcaaaa actgaaacaa aacatctttt gttcttgcgc cgagagcttt ttttattcct
     4021 atttctctcg ttctttcagt aacggatacc agcatgatat tcattatgcc tattcccccc
     4081 accaggaggg atatggctgc gatggctgca accgcaactg ataagccggt cacaacactt
     4141 tccacctgtg tttgttcttc ctcaacggta taatatatgt agttttccgg tgtcctgttt
     4201 ttcattctgg caatataatt tattatctca tcacccgtaa tggccatgga atacttttca
     4261 tcaataaaaa tatccaaggc ataataggcg taatatgatg aagtaggcat agtataaggt
     4321 atgaagctgt tttcataatt gctttgtccc tgcatcaagg ttaaaagagg ggattgctct
     4381 attttatata ctcccacaac caggaggtct tccatattgt tgtcgatttt aaccctaatt
     4441 gtttttccta cagcattgtc cttcccgaag agctttaagg cggctttttg ctccaaaaca
     4501 actaccttgc tctttctttc tatatccttg ctatttatca tcctgccgta aagcatctta
     4561 atttgttgga ctttatcgta tccg
//
LOCUS       DAOSOW010000109         4576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_18943_length_4576_cov_3.439947, whole genome shotgun sequence.
ACCESSION   DAOSOW010000109 DAOSOW010000000
VERSION     DAOSOW010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18943_length_4576_cov_3.439947"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="PLD67_03980"
     CDS             complement(<1..1164)
                     /locus_tag="PLD67_03980"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HOT21710.1"
                     /translation="MLNLSFFGKSKVEYNGKEIGDRLGNKAIALICLLVLNERRYLSR
                     EKIIGYLWPDSNIEAAKYNLRYNLWLIKKNIAEDKNHNLFLKVDNDCCSINNNYEFNC
                     DIIDIMKFKPSREDSVESLLKLKKLFRGDLLEGCYFNKCDEFNDLIIYERINFEQRKV
                     RILQRLVEVYENDKRYDDCIEILYEIMEIEPYDEKIALKLMDIYQKSGKRAVAINYFN
                     KFSYSLSCDLGINPSNELKNKYNEIKMAVSGDEFNDETNYNVINKDTNLKIVSYCIKN
                     VEYFWMADVIDKIIDSGVEDCIQQLSQKQLLDLSSIQSSISKFCNDNIDIINYRREIM
                     DVCIINSFIKLVEAVCRKRNVTISILNYCDIDEISANVVEYLRKIKIKGLDIIE"
     gene            complement(1182..2717)
                     /locus_tag="PLD67_03985"
     CDS             complement(1182..2717)
                     /locus_tag="PLD67_03985"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011016557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOT21711.1"
                     /translation="MRADILIKNGRCMTMKDREIMDWIAIKKSKIIAVGHGLEYESFV
                     GNETVVFDVNGSTVLPGFIDSHFHMVQTALNSVSLDLSGVKTHEEVGEKIKEASNSFM
                     GDYIRGIRISEGQFQEKVYPGRIFIDKYCNDVPVVLNSMEYQVSILNTCALLYFKIPF
                     TAEGIEMDRKKMPTGIFRKHANAILRTNILKNISDSTRKDATSEIMSTLLMNGITTVN
                     AMEGGFMYCDKDAEFVFEHGNDFPIDMVLFYQTMDIDRIKAMGLNRIGGCLYIDGTFG
                     ARTAALSFEYADCPGTMGSLRMSQQELNDFVIQCYKNNLQLALYTIGDRAIESALIAH
                     ENALYQTGITGLRHRLEHAELVNDNQIERAKGLNIIFSMQPSYETYWGGRGKMYEQRL
                     GENYKRTNPFKKIIDKGVVICGGSDSDVTEPRPLLGIHSAVNHPVAEHRVDIYEALKM
                     FTCNGAYGIFEENNKGTLEVGKLADVVILDQDILSVPSNDIDKIKVNVTIKSGEILYN
                     SLG"
     gene            complement(2895..4097)
                     /locus_tag="PLD67_03990"
     CDS             complement(2895..4097)
                     /locus_tag="PLD67_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT21712.1"
                     /translation="MYKAMYKVDKAQITKLLIDLVNIESPYFEEDKIIEFIYRWFKQN
                     NIDSFIHEYHEAKVTGFKGKNIVVNLEGNKCGPVICLNGHLDTVKLCNGWTYNPKGEL
                     IGDRLYGVGALDMKSGCAATMIALKGFVENHKEFNGKIIATFVSVEEGPYGMGTNALI
                     EEGYIKDVDFSIITEPSSGFTGNPYPDVSFGARGGYGLEVEFFGKSSHAATPELGINA
                     AEDASRVITELKNVEYIEDPHLGKGSACVVAVESDGGACSVPDYAKIRLFWHIVMGES
                     EETITNEIRKAIERAQIKSSYQINFREAPSEGSRGFLPYTITGEEPITKIFLDAVEEV
                     CETKPTISYFQSIGDFNYLGSRLDAPAVIFGAAGGNFHGSDEYVELESVVKTAEVIYA
                     FLEKVLCP"
     gene            complement(4140..>4576)
                     /locus_tag="PLD67_03995"
     CDS             complement(4140..>4576)
                     /locus_tag="PLD67_03995"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOT21713.1"
                     /translation="KSDAMETLLDSMKGLTKNAKNLITTHVFSTIIINMLSASQYVSI
                     IIPGRMYLPAYRKLGIRTDVASRTCEDAGTVTSPLVPWGLCGVFFTGVLGVNTLEYLP
                     YAFLAYITPVIAILYAYTGKFIWKEEAGTVTELKSDTANGAK"
BASE COUNT         1405 a          956 c          605 g         1610 t
ORIGIN      
        1 ttcaattata tctagtccct taattttaat ttttctaaga tattcaacaa catttgccga
       61 aatttcatct atgtcacaat agtttaaaat cgatatagtc acatttcttt tacgacagac
      121 ggcttccacc agctttataa aagaatttat aatacacaca tccattattt ctcttctata
      181 attgattata tcaatattgt cattgcaaaa ttttgatata ctgctttgta tagagcttaa
      241 atctaacaat tgtttttgac tcaattgttg aatacaatct tctacacctg aatcaataat
      301 tttatcaatt acatcagcca tccaaaaata ttctacattc ttgatacaat aggaaacaat
      361 ttttagattg gtatctttat taatcacatt ataattagtt tcatcattaa attcgtcacc
      421 tgatacagcc attttgattt cattatattt atttttaagt tcattagagg gatttatacc
      481 aaggtcacag gatagactat agctgaattt attgaaataa tttattgcaa ctgcacgttt
      541 cccgcttttc tggtaaatat ccataagttt taatgctatt ttttcatcat aaggctcaat
      601 ttccattatt tcatataata tttcaataca gtcatcatat cttttatcat tttcataaac
      661 ttcaactaaa cgctgaagta tccttacctt gcgctgctca aaatttattc tttcgtatat
      721 aatgaggtca ttaaattcat cacacttgtt aaaatagcaa ccttccaata agtctccgcg
      781 aaaaagtttc ttaagcttta gtaagctttc tacagaatcc tctctgcttg gtttaaattt
      841 cataatatca ataatatcac aattaaattc ataattattg tttatactgc aacagtcatt
      901 gtccaccttc aaaaataggt tgtgattttt atcctcagct atatttttct ttataagcca
      961 taaattataa cgcaagttat actttgctgc ctcgatgttg ctatccggcc acaagtatcc
     1021 gataattttc tccctgctta aatatcttct ctcatttaac actaaaagac aaatcaaagc
     1081 tatggcctta tttcctaaac ggtctcctat ttctttacca ttgtattcaa ctttagattt
     1141 tccaaaaaaa cttagattca acatatcatt ttccttttta tttacccaag gctgttatac
     1201 aatatttcac cagatttaat agttacgttt acctttatct tatctatatc atttgaaggt
     1261 actgaaagga tgtcttgatc aagaattaca acatctgcca attttcctac ttctaaagta
     1321 cctttattgt tttcttcaaa aataccatat gcgccattgc aagtaaacat ttttaatgct
     1381 tcatatatat caactctgtg ttctgctacc gggtgattta cagcagaatg aatccccaaa
     1441 agaggtcttg gctctgtaac atcactatct gagccaccgc atataacaac acctttatcg
     1501 attatctttt tgaacggatt agttcgtttg taattttctc ctagcctttg ctcatacatt
     1561 tttcctcgtc cgccccaata tgtttcatat gatggttgca tagaaaaaat aatatttaaa
     1621 cctttcgccc tttcaatttg attatcatta actaattcag catgttccaa tctgtgacga
     1681 agacctgtta taccggtttg atacagtgca ttctcatgtg caataagtgc tgattctatt
     1741 gctctgtcac cgattgtata caatgctaac tgcagattgt ttttatagca ttgtattaca
     1801 aaatcattta gctcctgctg tgacatacgg agactgccca tggttcccgg acaatctgca
     1861 tattcaaagg aaagagctgc tgttcttgcg ccaaaggttc catcgatata gagacatcct
     1921 ccaatcctgt taagccccat tgctttaatt ctatcaatat ccattgtctg ataaaaaagc
     1981 accatatcta tcgggaaatc atttccatgc tcaaaaacaa actctgcatc cttgtcacag
     2041 tacataaatc cgccttccat ggcatttacg gtagtaatac cattcattaa aagagtagac
     2101 ataatttcgg aagttgcatc tttccttgtg ctatccgata tgttttttaa tatatttgtt
     2161 ctaagaatag cattagcatg ttttctgaag attcctgtag gcatcttttt tctgtccatt
     2221 tcaattcctt cagctgtaaa aggtatttta aaatataaca aggcacaagt attaagaatg
     2281 cttacctgat actccatgga attaagaacg accggtacat cattacagta cttatcaata
     2341 aatattcttc ccggatatac tttttcttgg aattgtcctt ctgaaattcg aattccgcga
     2401 atataatctc ccataaagct attggaggct tccttaattt tttcgccaac ttcttcatga
     2461 gtttttactc cactcaaatc taaacttaca gaattaagag ctgtctgaac catatgaaaa
     2521 tgactatcaa taaatcccgg aagtaccgta ctaccgttca catcaaaaac aactgtttca
     2581 tttccaacga aagattcata ttctaagcca tgtcctaccg caattatttt acttttttta
     2641 atggcaatcc agtccataat ttctctatct ttcattgtca tgcatcttcc atttttaatc
     2701 aaaatatccg cacgcatctt aattcctcca aaaaatgtcg tttcattttt atgcagttga
     2761 ataattaaat ataacaaata ttataccagt ataaaaccga tttttcaata gaataaaaaa
     2821 gccggtaaag cttttattta attaatgata aaagtctaag aaaagcttga tggcttttct
     2881 tagaccttaa aaaattatgg gcacagtacc ttctcaagaa aagcataaat aacctctgca
     2941 gtttttacga ctgattccaa ttccacatat tcatcacttc catggaagtt accgcctgca
     3001 gctccaaaaa ttacagcagg tgcatccaat ctggagccta aataattaaa atctccaata
     3061 ctttgaaaat aagatatagt aggttttgtt tcgcacactt cttcaacagc gtccaaaaag
     3121 atttttgtta taggttcttc tcccgtaata gtataaggca aaaaacccct ggaaccttca
     3181 gaaggtgctt ctctgaagtt aatttgataa ctgcttttaa tctgtgctcg ctcaattgct
     3241 tttctgattt catttgtaat ggtttcttca ctttctccca ttacaatatg ccaaaacaat
     3301 cgtatttttg cataatcagg aacactgcag gcaccaccgt cgctttcaac agccaccaca
     3361 caggcagaac ccttcccgag atgggggtct tcaatatatt ctacattttt taattcggtt
     3421 attactcttg aagcatcttc tgctgcatta attcccaatt cgggagtggc tgcatgggat
     3481 gatttgccaa aaaattcaac ctcaagacca tatccacccc gagctccaaa gctgacatcc
     3541 ggatatggat ttccggtaaa gcctgaagat ggctcagtga taattgaaaa atctacgtcc
     3601 tttatatagc cttcttcaat aagggcatta gttcccatgc catacggacc ctcttcaaca
     3661 gaaacaaatg ttgcaattat tttcccgtta aactctttat ggttttcgac aaatcccttc
     3721 aatgcgatca tagttgctgc acagcctgac ttcatatcca atgctccaac tccatatagt
     3781 ctatcgccta ttagttctcc cttcggatta taagtccaac cgttacaaag cttaacagtg
     3841 tctaagtgcc cgttaagaca tattacagga ccacacttat tgccttctaa atttacaaca
     3901 atatttttac ctttaaaacc ggtaactttt gcttcatgat actcatgaat gaaagaatct
     3961 atattatttt gcttaaacca gcggtagata aactctataa ttttgtcttc ctcgaaataa
     4021 ggactttcaa tattgactaa gtctatcagc aattttgtta tttgagcctt atcgacttta
     4081 tacatagctt tatacatacg ctcacctatt gtcctttaac ataacttata atattttcat
     4141 cattttgcac cattggcagt atccgacttt aattccgtta ctgtacctgc ttcctccttc
     4201 caaatgaact tcccggtata tgcatataga attgctataa caggagttat ataagccaag
     4261 aatgcatatg gaagatactc taaagtattt actccaagta ctcctgtaaa gaatacgcca
     4321 cacaatcccc aaggtaccaa gggagatgtc accgtacctg catcctcaca tgttcttgaa
     4381 gctacatcag ttcttatgcc taattttcta tatgcaggaa gatacattct acccggtata
     4441 ataattgaaa catactggct tgcagataac atatttatga taatcgtaga aaatacatgt
     4501 gtagtaataa ggttttttgc atttttcgtg agtcctttca tactgtcaag caatgtttcc
     4561 attgcatcgg attttt
//
LOCUS       DAOSOW010000110         4566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_19004_length_4566_cov_3.327422, whole genome shotgun sequence.
ACCESSION   DAOSOW010000110 DAOSOW010000000
VERSION     DAOSOW010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19004_length_4566_cov_3.327422"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="PLD67_04000"
     CDS             complement(<1..428)
                     /locus_tag="PLD67_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOT21714.1"
                     /translation="MNKDTSKVYTVSFITLGCKVNQFETEAMTEILERDGFKVLPPNE
                     VSDIYIINTCAVTKESERKSRQFINKAKRINPDALVVAVGCSVQLNSEKIKKETQADI
                     IIGTKHKGNIGKLIKNKLNDIKQECKIDDFRGRESFEELK"
     gene            complement(654..998)
                     /locus_tag="PLD67_04005"
     CDS             complement(654..998)
                     /locus_tag="PLD67_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOT21715.1"
                     /translation="MYNKNKGFEYEKIAEKYLQNNNYKILEVNFISKFGEIDIIAEKD
                     NRLHFIEVKGRKNIKHGYPKEAVTFSKQKRLRSAAKYYFMLKGKDDYLCQFDVIEIIL
                     ESRDINHIKNAF"
     gene            complement(998..2509)
                     /locus_tag="PLD67_04010"
     CDS             complement(998..2509)
                     /locus_tag="PLD67_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOT21716.1"
                     /translation="MFADNKIWIGKSDKKVYLEPKMANRHGLVSGATGTGKTITLKVL
                     AESFSDLGVPVFIADIKGDLASIAIPGTDNENMQERLNRLEINNFIYKGYPVQFWDVY
                     GEGGIPVRTTVTEMGPLMLSRILNLNDTQTGVLNIVFRVADERGLLLLDLKDLRAMVQ
                     YVGDNSKDFRMKYGNITAQSIGAILRSLMTLEDQGGEFFFGEPELDIFDWIKTSADGR
                     GYINVLHCAKLFLNPLLYSTFLLWMLSELYENLPEEGDLEKPKIVFFFDEAHLLFEDA
                     PKQLLDKIELVVRLIRSKGVGVYFVTQNPIDVPNDILGRLGNRIQHALRAYTPAERKK
                     VVTAAETFRENPEFNTVEAITELGTGEALISCLDEKGIPSVVERAFILPPQSRFGTID
                     DMLRNQIIAGTPIYKKYYKSVDRESAYEILLAKTERKYQEELLETERLEEEKRLKLEA
                     KERERQQKEFERARKRASSKTTAIDRVANSAMNAIGREVGRSLIRGVLGTFKR"
     gene            complement(2526..3479)
                     /locus_tag="PLD67_04015"
     CDS             complement(2526..3479)
                     /locus_tag="PLD67_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT21717.1"
                     /translation="MSTVYFLAQQTMYFAIPLLIVALGGMFTERSGIVNIALEGIMVV
                     GAFFGIGFIHIFQDSMSGQSLLLLAVLVSALTGAIFSLFHAFASINLKANQVISGTAL
                     NMFAPAFAIYTARMIQGVQQVQFMDTFYIRSVPILGDIPILGPIMFKNAYITTYIGLL
                     IALIAWFIINHTRFGLRLRACGEHPQAADSVGINVYHIRYAGVIISGILAGIGGLTFV
                     VPTSTNFNATVAGYGFLALAVLIFGQWRTKKIFVAAFFFGIMKTFASAYSGIPFLKGL
                     PISNEVYKMIPYIATLIVLTFSSKKSQAPRAEGIPYDKGSR"
     gene            complement(3476..>4566)
                     /locus_tag="PLD67_04020"
     CDS             complement(3476..>4566)
                     /locus_tag="PLD67_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008727541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT21718.1"
                     /translation="LISNSEDAFPAFLTILKGGFVDGSRGLAQVIYFAIPIIMTGLSV
                     GFAFKTGLFNIGASGQFTLGAFVAIYIGIKWTFVPPEIHWLVALLGAMVAGAVWGSGP
                     GILKAFFNVNEVITSIMMNYIGMYLVNMSIVKTVYDSLKNQTKPVLKSAVLPKAGMDK
                     LFNTSNVSIGIFIVILAVIVIYIILQKTTFGYELKACGSNKDASKYAGINEKRSIVLS
                     MVIAGALSGLGGGLLYLSGSGKYIHVLDVLAPEGFSGISVSLLGMSHPIGIFLAGLFI
                     AHLEVGGFNIQLYNFVPEVIDMIIAVIIYCGAFALLFKQIISKFIIHDTDEDIGKSVQ
                     AADIPASYESSVVNAETEEKTEQREDKK"
BASE COUNT         1375 a          991 c          652 g         1548 t
ORIGIN      
        1 atttttaatt cttcgaagct ttctcttcct cgaaaatcat caattttaca ttcttgtttt
       61 atatcattta acttattctt aataagtttc cctatattac ctttatgttt tgtcccaata
      121 attatgtccg cttgggtttc tttttttatt ttttcggaat ttaattgtac gctgcaaccc
      181 actgccacaa ctaatgcatc agggtttatt ctttttgcct tgtttataaa ttgcctggat
      241 ttcctttcgc tttccttagt aaccgcacat gtatttatta tatatatatc gcttacttca
      301 ttgggaggca aaactttaaa tccgtctctt tctaatattt cggtcatagc ttccgtttca
      361 aactgattta ctttacaacc caaggttata aaagaaacgg tgtatacttt acttgtatcc
      421 ttattcattc ttgctccttg caataaattt cttgcatgat aataacatag ataattaatt
      481 aaatcaacca gtaaaattaa tagaaaatta atggtgccaa aattaatggt gccaaattaa
      541 tggtgccagg caccagtatt tgcaggcacc agtatttaga tgacagtacc cccgtcccct
      601 tgtcatacgt ccccttgtca taaaaacaag ttcagagtaa tctgaacttg gtgttaaaaa
      661 gcatttttta tatgatttat atctctgctt tccaatatta tttctataac gtcaaattga
      721 catagatagt cgtcttttcc tttcagcata aaataatatt tagcagcact tcttaatctt
      781 ttttgtttgg aaaatgttac tgcctcttta ggatatccgt gcttaatatt tttgcgccct
      841 tttacttcaa tgaagtgcag cctattatct ttttcggcaa taatatctat ttcaccaaat
      901 ttgcttataa agttaacttc taatatttta taattattat tttgtaaata tttttcggca
      961 attttctcgt attcaaaacc cttattttta ttatacatta tcttttaaag gtgcctaaaa
     1021 ctcctcttat aagcgaacgt cccacttctc ttccgattgc attcatggct gaattagcta
     1081 ccctgtcaat ggcagttgtt ttgctgcttg ctctttttct ggctctttca aattcttttt
     1141 gttgtctttc tctttcctta gcctctaatt tgagtctttt ttcttcttca agtctttctg
     1201 tttcaagaag ttcttcctga tattttcttt ccgttttagc taataaaatt tcataagcgg
     1261 actctctgtc aactgactta taatatttct tataaatcgg agtaccggca attatttggt
     1321 ttctaagcat atcatctatc gttccaaacc ggctttgcgg cggaagtata aatgcgcgct
     1381 caacaacaga aggtattccc ttttcatcaa ggcaagaaat taatgcttca cctgttccaa
     1441 gctcggttat tgcttccact gtgttaaatt caggattttc acggaatgtt tcagcagccg
     1501 taacaacctt ttttctttca gccggtgtat atgctcttaa agcatgctga attctgtttc
     1561 ctaatcgtcc caatatgtca ttaggcacat cgattggatt ttgtgtcaca aaataaacac
     1621 ccacaccttt ggagcgtatt aaccgaacca ccaattcaat tttatcaagc agttgttttg
     1681 gagcatcttc aaaaagcaga tgtgcttcat caaagaaaaa tacaatttta ggcttttcca
     1741 aatccccttc ttcaggaagg ttttcataca attccgacag catccacaac aaaaatgttg
     1801 aatacaacaa gggatttaaa aataattttg cacagtgcaa aacatttata taacctcttc
     1861 cgtctgcaga tgtttttatc cagtcaaata tatccaattc cggctctccg aagaaaaatt
     1921 caccgccctg gtcttccaag gtcatcaagc ttctcaaaat agcaccgata ctttgagccg
     1981 ttatgtttcc gtatttcatt ctgaaatcct ttgaattgtc tcccacgtat tgtaccatag
     2041 cacgcagatc ctttaaatcc aacaacaata gtcctctctc atctgccact ctgaatacta
     2101 tattcaaaac acctgtctgt gtatcattta aatttaaaat ccttgacagc atcaacggac
     2161 ccatttctgt aaccgtagta cgtacaggta ttcccccttc accgtataca tcccaaaatt
     2221 gaacagggta tcctttgtat ataaagttat ttatttctaa acgattgagc ctctcctgca
     2281 tattttcatt gtctgttccc ggaattgcaa tgcttgccaa gtctccttta atatcagcaa
     2341 taaaaacagg aacgcccaaa tcactaaagg attctgccaa tacttttaat gtaatagttt
     2401 ttcccgtgcc tgttgctcct gacaccaaac cgtgtctgtt agccatttta ggctctaaat
     2461 atactttttt atcacttttt cctatccaaa ttttattatc ggcaaacata gtatctcctt
     2521 ttatattatc tgcttccttt atcatatggt attccttctg ctcttggtgc ctgagatttc
     2581 ttggaggaga atgtcaatac tattaaggta gctatataag gaatcatttt ataaacttca
     2641 tttgatatcg gcaaaccttt aagaaagggt atacctgaat aagcagaagc aaatgtcttc
     2701 attattccaa agaaaaatgc agcaacgaat attttcttcg ttctccactg accgaaaatc
     2761 aaaactgcca atgccaagaa tccatatccg gcaacagttg cattaaaatt tgtactagtc
     2821 ggcactacaa atgtaagtcc tcctattccg gctaatatac ctgatataat tacacctgca
     2881 tatctaatat ggtatacgtt aataccgact gaatctgcag cttgcggatg ttccccgcag
     2941 gctcgaagcc tcaggccgaa acgcgtatga tttattataa accatgcaat tagagcaatc
     3001 aacagtccta tatatgttgt aatgtaagca ttcttgaaca ttatggggcc taatatcggt
     3061 atatctccca aaatcggaac actccttata taaaatgtat ccatgaattg aacttgctga
     3121 accccctgaa tcattctagc agtataaatt gcaaaggcag gagcaaacat attcaatgct
     3181 gtaccgctga taacttgatt tgcttttaaa ttaatcgatg caaatgcatg aaacagtgaa
     3241 aatatcgcac ctgtcaaggc agataccaat acagccaata acaataggct ttgaccgctc
     3301 atagaatctt gaaatatatg aataaacccg attccaaaaa aagcccctac taccattatt
     3361 ccttctaaag caatattaac aataccgctt ctttccgtaa acattcctcc caaagcaact
     3421 atcagaagag gaattgcaaa atacatagtt tgctgtgcca aaaaataaac cgtactcatt
     3481 tcttatcctc cctttgctct gttttttctt cagtttcagc attaacaacc gaggattcgt
     3541 aagaagcagg aatatcggct gcctgcaccg atttcccaat gtcttcatcc gtatcatgaa
     3601 ttataaattt gcttattatt tgtttaaaca ataatgcaaa agctccgcag taaataatta
     3661 ctgcaataat catatctatt acttccggta caaaattata gagctgaata ttaaaaccgc
     3721 ctacttctag atgagctata aataatccgg caagaaatat tcctatggga tgggacatac
     3781 ctaaaagcga gactgaaata ccgctgaacc cttccggcgc caatacatcc aacacatgga
     3841 tatacttacc agagcctgac agataaagaa gtcctcctcc taatcctgat agagctcctg
     3901 caataaccat tgataacact atacttctct tttcattgat tcccgcatat ttgctcgcat
     3961 ccttattgct gccgcatgcc tttaattcgt atccaaaggt agttttctgt agaattatat
     4021 agataactat tacagctaaa attactatga atattccaat actgacatta ctagtgttaa
     4081 aaagcttatc cattccggcc tttggcaata ctgcgctctt taaaaccggt tttgtttggt
     4141 tttttaaaga atcgtacaca gttttcacta ttgacatatt aaccaggtac attcctatat
     4201 aattcatcat tattgatgta ataacttcat taacattgaa aaatgctttc agtataccgg
     4261 gaccggagcc ccataccgct cctgcaacca ttgcccccaa taatgcaaca agccaatgta
     4321 tttccggtgg aacgaaggtc catttaattc ctatataaat agcaacaaag gcacctaagg
     4381 taaattgtcc tgaagctcct atgttgaaca atcccgtttt aaaagcaaat cctacggata
     4441 aaccggtcat aataatgggt atggcaaaat atataacttg agccaatccc cttgagccgt
     4501 ctacaaaacc tccttttaaa atcgttaaaa aggcaggaaa agcatcttct gaattactta
     4561 ttaaca
//
LOCUS       DAOSOW010000111         4525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_19238_length_4525_cov_4.750336, whole genome shotgun sequence.
ACCESSION   DAOSOW010000111 DAOSOW010000000
VERSION     DAOSOW010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4525
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19238_length_4525_cov_4.750336"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PLD67_04025"
     CDS             complement(<1..917)
                     /locus_tag="PLD67_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT21719.1"
                     /translation="MNNLDLIIKRRIFGIISHPDAGKTTLTEKLLLHGGAIRQAGTVR
                     ARKNARYAKSDWMEIEKQRGISVTSSVMQFEYDDKVISIMDTPGHNDFGEDTYRILTS
                     VDSAVMVIDAAKGIEAQTKKLFKVCSMRGIPIFTFINKLDREAKDPLELMQELEDVLG
                     LPSVAVTWPIGCGKEFEGIYDRQRNVVNLYKKKEIINLNEEDVKSNKLDGKIGPSNLT
                     NLRNAMELLDGAGNEFDIDLIRKGKLSPVFFGSALADFGVTEFLKHFLIMSPPPSSRK
                     TTAGEVHPTDDVFTGFIFNDNTITGIHTN"
     gene            1248..2570
                     /gene="miaB"
                     /locus_tag="PLD67_04030"
     CDS             1248..2570
                     /gene="miaB"
                     /locus_tag="PLD67_04030"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOT21720.1"
                     /translation="MKGRRKTYHILTYGCQMNEHDSEKISGMLTSIGYEETDDEKSAD
                     LVIFNTCLIRENAELKVFGKLGEVKGLKRNKPDMLVAVCGCMMQKQEIREKLLKKFSF
                     VDIIFGTNTIHELPLLIYNVEINKRKSMGIVDNTESIYENMPKQRKYKYKALVNITYG
                     CNNFCTYCVVPYVRGREKSREPNEIIDEIKTLAEDGCKEVTLLGQNVNSYGSNLDKRV
                     TFAELLYEINKIEGIERIRFMTSHPKDLTDDLIKAIKECNKVCNHVHLPVQSGSNDVL
                     NRMNRKYNREHYLNLVEKLKTAVPDIAITTDIIVGFPGETEEDFLDTIDIVKKVEYDS
                     AFTFLYSIREGTKAAKMENQVPDNVKHERFERLLEVLYPIILDKNKQCIGKVFPVLVE
                     SFENGKLTGRTEHFRLVHFNGSKDLIGKIVNVKITNVKTFHTEGEIVE"
     gene            2608..>4525
                     /gene="mutS"
                     /locus_tag="PLD67_04035"
     CDS             2608..>4525
                     /gene="mutS"
                     /locus_tag="PLD67_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOT21721.1"
                     /translation="MAKYTPMMEQYLAIKEQYKDCILLYRLGDFYEMFFDDAITASRV
                     LEIALTGRDCGQEERAPMCGVPHHAVDGYIAKLIENGYKVAICEQLEDPSDAKGIVKR
                     DVVRIITPGTIIEQGMLDEKSNNYLCTIYLDKGFGMSYVDISTGELYVTENISLNDFL
                     KNSTHDLLIEEINKITPSEIISNNHIGNEVIDSMIYLSNVLSLDDYKQIVLKHFKVLS
                     LDSLGLKENYHAVISLGMLLNYLNETQKTSLDHINKLHFYTVGEYLQIDANTRKNLEL
                     IETIRGKKGPGTLYHVVDNTMTAMGGRLLKKWIEEPLKNIKHIYLRLDAVEELYENIM
                     TSNNIKEYLKSVYDIERLISRIVYGSCNGRDLNSLKQSLSNLPDLKSELNELSSQMFA
                     DIYSNFDELKDIFDLIDASIVEEPPISIKEGGIIKTGYNEELDEIREITVNGKDWISN
                     LQAKEREATGIKNLKIGFNKVFGYYLEVTKSYLKDVPDNYIRKQTLANCERYVTPELK
                     EMEAKILNADEQIMKLEYELFLEIREFISRHVKRIQETAYNIAVIDAINSLAIAAVKN
                     NYVRPEMNKKGFIKITDGRHPVIERIMKNEMFVPNDTYIDNKEYRMSIITGPNMAGKS
                     TYMRQVALIALMSHI"
BASE COUNT         1616 a          682 c          868 g         1359 t
ORIGIN      
        1 ttatttgtat gaatgccggt tatggtatta tcattaaaaa taaaacccgt aaatacatcg
       61 tccgtcggat ggacttctcc tgcagttgtt tttcttgatg atgggggagg agacataata
      121 agaaaatgct ttaaaaattc tgttactcca aagtctgcta aggcagagcc gaagaataca
      181 ggtgataatt tgccttttct gattaaatct atatcaaatt cattacctgc accgtcaaga
      241 agctccatgg catttctcaa atttgtcaaa ttggaaggac cgatttttcc gtctaatttg
      301 tttgatttaa catcttcttc attgagatta ataatttctt tctttttgta taagttaact
      361 acatttcttt gcctgtcata aattccttca aattctttgc cgcatccgat aggccatgtt
      421 acagccaccg aaggaagtcc cagtacatcc tctaattcct gcattaattc cagcgggtcc
      481 tttgcttcac ggtctaattt gtttataaat gtgaatattg gaattcctct catgctgcaa
      541 accttaaaca gcttctttgt ttgcgcttct ataccttttg cagcatctat aaccattacg
      601 gcactgtcaa cagaggttaa aatacgataa gtatcttcac caaagtcatt gtgtccggga
      661 gtatccataa ttgaaattac tttatcatca tattcaaact gcataactga agaagtaacc
      721 gatatacctc gctgtttttc tatttccatc cagtctgatt tagcatatct ggcatttttt
      781 cttgcgcgaa ccgtacctgc ctgcctgatt gctcctccgt ggagcaggag cttttcggtc
      841 aacgttgttt ttcctgcatc agggtgagat attataccaa atattcgtcg tttgataatc
      901 aaatctaaat tattcatttt tttcctttct agccggtaat aattaatctt taatattcta
      961 cactatttcg acaaaaatat aaagaaatat ttacaagtaa ttttgattcg caaggatggt
     1021 acactaattc tgaaatttag attcaatagt atcttgtata aaggctgagg gaattttttt
     1081 attgaacaaa aattcgtaaa atgatataat attcaagatg ctaaacgttt agattagaaa
     1141 gttggatata gatggacgaa agaaatattg atattataac agaagatata aaattagacg
     1201 gcgataaatt attagaattg aagaacaata atgagcaatt cttcataatg aagggcagaa
     1261 gaaaaactta tcatatattg acatacggct gccaaatgaa tgaacacgac tctgaaaaaa
     1321 tatccggtat gctgacatcc atcggttatg aagagactga tgatgaaaaa agtgcagacc
     1381 ttgtaatttt taatacatgt ttaataaggg aaaatgcgga acttaaagta tttggcaaac
     1441 ttggtgaagt aaaaggactt aagcgaaata aaccggatat gctggttgca gtttgcggct
     1501 gtatgatgca aaaacaagaa ataagagaaa aactgttaaa gaaattttca tttgtagata
     1561 ttatttttgg aacaaataca attcacgagc ttcctttgtt aatatacaat gttgaaatca
     1621 acaaaagaaa aagtatgggt attgttgata ataccgagtc aatatatgaa aatatgccca
     1681 agcaaagaaa gtataagtac aaagcattgg taaacataac atacggctgt aataatttct
     1741 gcacttactg cgtagttcca tatgtaagag gcagagaaaa atcaagagag ccgaatgaaa
     1801 taattgatga aattaaaaca ttggcagaag atggctgcaa agaagtaact cttcttggac
     1861 aaaatgttaa ttcttacgga agcaatttgg ataaaagggt tacatttgca gagcttcttt
     1921 atgaaattaa taaaattgaa ggtattgagc gcatacgctt tatgacatcc cacccgaaag
     1981 atttaacgga tgatttgata aaagcaatta aagaatgtaa taaggtatgc aaccacgttc
     2041 atttgcctgt tcaatcggga agcaatgatg tgctaaatcg catgaaccgt aagtacaata
     2101 gggagcatta tttaaacttg gttgaaaaat taaaaacagc tgtacctgac attgcaataa
     2161 caacagatat aattgtagga ttcccgggag aaacagagga agatttcttg gatactatag
     2221 atattgtcaa aaaggtcgag tatgattctg cttttacttt tctttattcg ataagagagg
     2281 gtacgaaggc agccaaaatg gagaaccaag tgccggataa tgtgaaacat gaacgttttg
     2341 aaaggctttt ggaagttttg tacccgataa tactggataa gaataaacag tgtatcggta
     2401 aagtatttcc cgtattggtt gaatctttcg agaacggtaa attaacaggc aggactgagc
     2461 atttcagatt agttcatttc aatggaagta aggatttaat cggtaaaatt gtaaatgtaa
     2521 aaataacgaa tgtaaaaact ttccacactg agggagaaat agtcgagtaa taaggtatta
     2581 atagacaatg gtagaagggg attgtaaatg gcaaagtata cacctatgat ggagcaatat
     2641 cttgcaatta aagagcaata taaagactgt attcttttgt acaggcttgg agatttttat
     2701 gaaatgtttt ttgatgatgc tattacagct tcaagggtat tagagattgc acttacggga
     2761 agagattgcg gacaagaaga aagggctccg atgtgcggtg tcccgcatca tgccgttgac
     2821 ggatatattg caaagcttat agaaaacggc tacaaggttg caatatgcga gcagcttgaa
     2881 gacccttcgg atgccaaagg aattgtaaaa cgcgatgtag taagaataat aactcccgga
     2941 acaataatcg agcaaggtat gctggatgaa aaaagcaaca attatttgtg cactatatat
     3001 ttggacaagg gttttggtat gtcttacgtg gacatttcaa cgggagagct ctatgtaact
     3061 gaaaacatat cgttaaatga ttttttgaaa aatagcactc acgatttatt aatagaagaa
     3121 atcaacaaaa taaccccgtc agaaattata tccaacaacc atatcggtaa tgaagttata
     3181 gattcaatga tatatttaag caatgtattg agcttagatg actataagca aatagttttg
     3241 aagcatttta aggtactatc attggactca ttagggctta aagagaatta ccatgcggta
     3301 atatctcttg gaatgctttt aaattattta aatgaaactc aaaaaacttc actcgaccac
     3361 attaataagc ttcattttta tacggtgggg gaatacctgc agattgatgc aaacacaaga
     3421 aaaaatttgg agcttattga aactattaga gggaaaaaag gccccggaac actctaccat
     3481 gttgttgaca acaccatgac tgccatgggg ggaaggcttc ttaaaaaatg gattgaagag
     3541 cctcttaaaa atattaagca tatttattta agattggatg cagttgaaga attatatgaa
     3601 aatataatga cttctaataa tattaaagaa tatttaaaaa gtgtttatga tatagaaagg
     3661 cttataagcc gaattgtata cggctcttgt aacggaaggg atttaaattc tttaaagcaa
     3721 tccctctcaa atttaccgga tttaaaatca gaattaaatg agctttcttc tcaaatgttt
     3781 gccgatattt attccaattt tgatgagctt aaagatattt ttgatttaat tgatgcgtcg
     3841 attgtagaag agcctcccat atcaattaaa gagggaggaa taataaaaac aggctataat
     3901 gaagagttgg atgaaataag ggaaattacg gtaaacggta aggattggat ttccaacctt
     3961 caagcaaaag aaagagaagc aaccggtatt aagaatttaa aaatcggttt taacaaggtt
     4021 ttcggctact atttggaggt tacaaaatca tatttaaaag atgttcccga taattacata
     4081 cgaaagcaga ctttagccaa ctgcgaaaga tatgtaaccc ccgaacttaa agaaatggaa
     4141 gctaaaattt tgaatgccga tgaacaaata atgaaactgg aatatgaatt gttcttggaa
     4201 ataagagagt tcataagcag gcatgtgaaa agaattcaag aaacagcata taatattgca
     4261 gtaatagatg caattaattc acttgcaatt gctgccgtta aaaataatta tgtgcggcct
     4321 gaaatgaaca agaaaggttt tataaaaatt accgacggaa gacatcctgt tattgaaaga
     4381 attatgaaaa acgagatgtt tgttccaaat gacacataca tagataacaa ggaataccgc
     4441 atgtcaatta tcacaggtcc gaatatggca ggcaaatcca cttatatgag gcaggttgca
     4501 ttaattgcac taatgtctca tatag
//
LOCUS       DAOSOW010000112         4479 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_19488_length_4479_cov_4.017179, whole genome shotgun sequence.
ACCESSION   DAOSOW010000112 DAOSOW010000000
VERSION     DAOSOW010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19488_length_4479_cov_4.017179"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PLD67_04040"
     CDS             <1..552
                     /locus_tag="PLD67_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase domain-containing
                     protein"
                     /protein_id="HOT21722.1"
                     /translation="KDLFDIVIKPTVPNELLDENTKYIVNPTGRFVIGGPQGDSGLTG
                     RKIIVDTYGGYSRHGGGAFSGKDPTKVDRSAAYAARYIAKNVVAAGLADKCEVEVAYA
                     IGVAKPVSIMVETFGTNKVDDEIIQKAITENFDLRPAAIIEKFNLRRPIYQKLAAYGH
                     FGREDLDLPWEKTDKTDELKKYL"
     gene            603..2099
                     /gene="malQ"
                     /locus_tag="PLD67_04045"
     CDS             603..2099
                     /gene="malQ"
                     /locus_tag="PLD67_04045"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002596715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOT21723.1"
                     /translation="MFERSTGILLPIFSLPSKYGIGTLGKEAYKFIDFLRSAGQKYWQ
                     ILPIGHTSYGDSPYSCFSVFAGNPYFIDLDVLFEEGFIEETDLKGIDFGSDDERIDYK
                     KIFETRYGILKKAYLNKGMSCDLASFTCNNPWVSDYALFMSLKYKNNQLPWYKWDEMF
                     IKRDINTLQKYKNDFYDEINFWIFLQYIFFEQYFKLKDYANSSGISIIGDIPIYVAED
                     SVEAWTEKKLLMLNEDNLPICVAGVPPDDFSDDGQLWGNPVYNWEYLKETGYMWWIDR
                     IAFSFKLFDVVRIDHFRGFDEFWAVPYGSENAVNGSWMPAYGKQMFQTLKKSLGNLNI
                     IAEDLGIITDSVIKLKNDSGYAGMKVLQFAFDGNNDNPYLPYNYEENDVAYTGTHDND
                     TLKGWFNKLTLKEKQYVLQKLNINMVNEKDDNNLVYELIKAIYKSKASLCIVPLQDFL
                     CLGSEARVNIPSTLGNNWRWRLKNSMITDELAEKIKALKTERDVQNFN"
     gene            complement(2285..4057)
                     /locus_tag="PLD67_04050"
     CDS             complement(2285..4057)
                     /locus_tag="PLD67_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOT21724.1"
                     /translation="MYKFNSQSEFYRYPQGGIKSNEIITFKILVKRNICAPELLIEKR
                     HDYSRTFHDKIKMHWISQEKNYDIYQAMLSIEEYGHYYYSFIFDNEKSKDYELLIYDD
                     KYITPNWAKGGIIYHIFVDRFFRTKIIKKKGAIIRNWGETPYYLPDKKGEILNNDFFG
                     GNLDGIREKLPYIAKLGVSIIYLSPIFEAYSNHKYDTGNYHKIDPMFGTENSLKKLIK
                     EAKKYNIHVILDGVFSHTGDDSIYFNKYGRYDSIGAYQSKDSPYYDWYTFNNWNDDYV
                     SWWGIKTLPEVNENNDSYINFITGKNGVITHWQKTGIMGWRLDVADELPDEFIIALRN
                     SIKKNNEDALIIGEVWEDASNKYSYNKLREYLCGKQLDSITNYPLRNGIIEFVKNKDC
                     TNLNHIMNKVIETYPPDTVNCLMNVLGTHDTERILTILGSKRVPSSRSEMAESKLSYE
                     ELEQGIKFLKIAVLLQMTLPGIPCIYYGDEAGIEGWDDPFNRRCYPWGKENKEILDFY
                     LFITKLRRTNKIFAEGKYRCLIHDKEVFVFERFNENERIIIAVNLSQKEITLKFKEDI
                     NEYTNNIKNNIFNIEKENYLILLS"
     gene            complement(4136..>4479)
                     /locus_tag="PLD67_04055"
     CDS             complement(4136..>4479)
                     /locus_tag="PLD67_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOT21725.1"
                     /translation="ELWKNGYSSVIYYQKSLMLRKIIDFLNKGISGKSFESISRYLIG
                     GPVVDDPYMCFADFDSYMEVHQKASQLYFNNKKKWNQMSLMNIASAGVFSSDRTIREY
                     ADNIWGIKPVV"
BASE COUNT         1458 a          714 c          769 g         1538 t
ORIGIN      
        1 aaagaccttt ttgacattgt aataaaacca accgttccca atgaattgtt ggatgagaac
       61 acaaagtata tcgtaaatcc caccggaaga tttgttatag gcggccctca gggagactca
      121 ggattaacgg gaagaaagat aatagtagac acctacggcg gatattcaag acacggagga
      181 ggagcatttt caggaaagga ccccacaaag gttgaccgtt cagccgcata tgcagcaaga
      241 tatatagcta aaaatgtagt cgcagcagga ttggcagaca aatgtgaggt agaagtcgct
      301 tatgcaatcg gagtagcaaa accggtttca ataatggttg aaacattcgg tacaaacaag
      361 gttgatgatg aaattataca aaaagcaata accgagaatt ttgatttaag gcctgctgca
      421 attattgaaa aatttaattt aagaagacct atttatcaga agcttgcagc ttatggtcac
      481 tttggaagag aagaccttga tttaccatgg gaaaagacag ataaaacaga tgagttaaaa
      541 aaatatttat aaaagatatg gggacacttc ttttgaggaa tgtccccata ttaggagtat
      601 atatgtttga aagaagcacg ggtatcctat tacccatttt ttcacttcca tcaaaatacg
      661 gtatagggac tcttggaaaa gaagcttata aatttattga ttttttgcgc tcagccggac
      721 aaaaatactg gcagatttta cctataggcc atacttcata tggggattcg ccatattctt
      781 gtttttctgt atttgcagga aatccgtatt ttatagactt ggatgtattg tttgaagaag
      841 gattcattga agaaaccgac ttaaaaggta ttgacttcgg aagcgatgat gagcgaatcg
      901 attataagaa aatatttgaa actagatatg gaattttaaa aaaggcttat ttaaataaag
      961 ggatgtcctg tgatttagca tcttttacct gtaataatcc atgggtttcg gattatgcat
     1021 tgtttatgtc tttaaaatat aaaaataatc aacttccctg gtataaatgg gatgaaatgt
     1081 ttattaaaag agatataaat acacttcaaa agtataaaaa tgatttttat gatgaaataa
     1141 acttttggat ttttttacag tatatatttt ttgagcaata ttttaagttg aaggattatg
     1201 caaacagctc gggaatatca ataatcggtg atattcctat ttatgttgca gaggacagtg
     1261 ttgaagcatg gacagaaaag aagctgttaa tgttaaatga agataatctg cctatatgcg
     1321 ttgcaggtgt tcctccggat gatttttcag acgatgggca gctttgggga aatccggtct
     1381 ataattggga gtacttaaaa gaaacaggat acatgtggtg gatagacaga attgcatttt
     1441 catttaaatt gttcgatgtt gtaaggattg accatttcag aggatttgat gaattttggg
     1501 cagttcccta cggctctgaa aatgccgtaa acggtagctg gatgcctgct tatggaaaac
     1561 aaatgttcca aacattaaag aaaagtttgg gtaatttaaa tattattgca gaagatttgg
     1621 gaattattac cgacagtgtt attaaattaa aaaatgattc aggatatgcg ggaatgaaag
     1681 ttttgcaatt tgcatttgac gggaataatg ataaccctta tttgccttat aactatgagg
     1741 aaaatgatgt tgcatacact ggtactcatg ataatgatac attaaaggga tggtttaaca
     1801 aacttacgct taaagaaaaa caatatgttt tacaaaaatt aaatattaat atggttaatg
     1861 aaaaagatga taacaattta gtatacgagt tgataaaagc aatttataaa agcaaggcat
     1921 ccctttgtat agtacctttg caggattttc tttgcttagg ctcggaggca agagttaaca
     1981 taccgtcaac attgggcaac aactggagat ggagactaaa aaactcaatg attacagatg
     2041 aattagcaga aaaaataaaa gctttaaaaa ctgaaagaga tgttcaaaat ttcaattaat
     2101 gtgttaattt ataatgagtt tacctcaatt atcgacgtat taatctaaat ttagaatata
     2161 ttcgacctga aaaatatcag taaaatagca tttctatcaa aaaaaacaca caaaacttga
     2221 aattttgagc attcaaagtt tacagagcta tatattaata aaaaaaagaa ccgtccacaa
     2281 aatattatga tagtaaaatc aagtaatttt ccttttctat attgaaaata ttatttttta
     2341 tattatttgt gtattcattt atatcttctt tgaattttaa agtaatttct ttttgtgata
     2401 aatttactgc tataataatc ctttcatttt cattaaatct ttcgaacaca aaaacttcct
     2461 tatcatgaat taaacatctg tatttacctt cagcaaaaat tttatttgtt cttcgtaatt
     2521 ttgtaataaa taaataaaag tcaagtatct ctttattttc tttcccccaa ggatagcatc
     2581 ttctattgaa agggtcatcc catccttcaa ttcctgcttc gtcgccgtaa taaatgcagg
     2641 gaataccggg tagagtcatt tgcagcaaga ctgcaatttt taaaaactta attccttgct
     2701 ccaattcctc gtatgaaagt ttgctttctg ccatttcgct tctgcttgaa ggtactcttt
     2761 tgcttcccaa tattgtcagt attctttcag tgtcatgagt tcccagcaca ttcattaggc
     2821 agtttacggt atcaggagga tatgtttcaa taactttatt cattatatga ttaagattag
     2881 tgcagtcttt gttttttaca aattcgatta ttccgtttcg caggggataa tttgttatag
     2941 aatccaattg ctttccgcaa agatactctc tcagtttgtt gtatgaatac ttatttgaag
     3001 catcttccca tacttcaccg attatcagtg catcttcatt attctttttt attgaatttc
     3061 taagagcaat aatgaattca tccgggagtt cgtcagcaac atccaaacgc catcccataa
     3121 tgcctgtttt ttgccagtgg gtgataacac cgtttttccc tgttatgaaa tttatataag
     3181 agtcattgtt ttcattcact tcaggcaatg tttttatacc ccaccagctc acataatcat
     3241 cattccaatt attgaatgta taccaatcgt aataaggtga atccttgctt tggtaagcac
     3301 ctatggagtc gtacctgcca tatttattga aataaatgct gtcatcgcct gtgtggctga
     3361 aaactccgtc aagaataaca tgtatattat atttctttgc ttcttttata agttttttca
     3421 aggagttttc agtgccgaac atggggtcta tcttatgata atttcccgta tcgtacttgt
     3481 ggtttgaata tgcttcaaag attggcgata aataaattat gctaacacct aattttgcta
     3541 tgtagggcag tttttctctg ataccgtcta aatttcctcc aaagaagtcg ttatttaaaa
     3601 tctcaccctt tttatcagga agatagtatg gagtttcacc ccaattcctg attattgctc
     3661 cttttttttt gattatttta gtcctgaaaa acctatccac aaaaatatga tatataattc
     3721 ctcccttagc ccaatttgga gttatatatt tatcatcata tatcagcagt tcatagtctt
     3781 tggatttttc attatcaaaa ataaaggaat aatagtaatg accatattcc tctatggaaa
     3841 gcatagcttg gtagatatca tagttctttt cttgtgatat ccaatgcatt tttattttat
     3901 cgtgaaaagt cctactgtaa tcatgccttt tttcaatcaa taattcgggt gcacaaatgt
     3961 ttcgctttac aagtatttta aatgtaatta tttcatttga tttaattccg ccttgagggt
     4021 atctgtaaaa ttcagattgc gaattaaatt tatacataat gcctccttgt tgtaattaat
     4081 ctggggacat tcctcctact ccaatatcct cataatagag ttgtgtcccc acacattaaa
     4141 ctaccggttt aataccccat atattgtcgg catattctcg tattgttctg tcggaagaaa
     4201 aaacacctgc tgatgctata ttcattaatg acatttgatt ccacttcttt ttattattga
     4261 agtacagctg ggatgctttt tggtggactt ccatatagct gtcaaagtct gcaaaacaca
     4321 tgtaagggtc atccacgaca ggtcctccta tcaagtatct ggatatggat tcaaaggatt
     4381 ttcctgaaat ccctttgttt aaaaaatcaa taatttttct gagcatgaga cttttttgat
     4441 aatatattac tgatgaatat ccgtttttcc acaattctt
//
LOCUS       DAOSOW010000113         4471 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_19530_length_4471_cov_3.133379, whole genome shotgun sequence.
ACCESSION   DAOSOW010000113 DAOSOW010000000
VERSION     DAOSOW010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19530_length_4471_cov_3.133379"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PLD67_04060"
     CDS             <1..812
                     /locus_tag="PLD67_04060"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOT21726.1"
                     /translation="GYSLSESSDMLSAEGIRLYLNEEFKNIPITVYKTIDSTNAKAKL
                     LAVQNAEHGTTIIAEEQTKGRGRFGRDFFSPAESGIYMSIILKAELSIENSVLVTTAA
                     AVAVCQAIEKFTSVYPKIKWVNDIFIGDKKICGILTEAVTNFESGMMDCVVVGIGINV
                     KTRNEEFPLELQNTAGSIFIDDDNYIRNQLAAEIINNLLKAANNLADKSFMKSYKQRS
                     MVLGENILYKKDNKWHEGYALDIDDYGGLVIYTEDGQKITLNSGEVSIKKK"
     gene            893..1444
                     /locus_tag="PLD67_04065"
     CDS             893..1444
                     /locus_tag="PLD67_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HOT21727.1"
                     /translation="MYKEQTFSVRDITQIGIFTALTAVGAFISIPIGPVPITLQSFFV
                     LLSGIILGSKKAMFSQITYLLLGLIGFPIFSGFSGGLQHIFKPSFGFIIGYVAAAYVV
                     GKLTEEKTASQYLWAAVFAGTLIIYTFGLPYMYYILNIMLNSNLSIVKILQLGMFAFI
                     PGDMLKAVIIVFFAQKISPLLKG"
     gene            complement(1433..2764)
                     /locus_tag="PLD67_04070"
     CDS             complement(1433..2764)
                     /locus_tag="PLD67_04070"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT21728.1"
                     /translation="MRVVIIGAVAAGTSAATEIRRNDKEAEIIIYEKDKYISYAGCGM
                     PYYISNEAEGFSSVVPRDKDFSKEKHNIDIYTEHEVVSVNPNNKTLRVKNKSGEIMDR
                     YDKLIIATGAMSVKPDLKGSDRDNVFLLRNINDMIDIKNFIDENKPKSAVIIGSGFIG
                     LEMCESFKLLGLDVTIIARSKISKGIDNDLSLYIEEHLKEKGVNVYTNTKTVEINDSG
                     VVLGDGSIVKADMVLLATGAKPNVTLAKEMGVELGATGAIKVDKYMKTNIEDVYSCGD
                     CIEMYYSFNGNPVYRPLGSTANKTGTIAGSNITGIEQEFKGILGTAIYRVFELTVGMT
                     GLSEEEAVKSGYNVVITIDKRPNKPEYMGGRPIIIKTVAEKGTRRLLGAQLIGYEGVD
                     RRLDVLVTAITFNAKAEDLMHLDLAYAPPYSTPRDNLYYTGAKLKAKLSAL"
     gene            complement(2794..3273)
                     /locus_tag="PLD67_04075"
     CDS             complement(2794..3273)
                     /locus_tag="PLD67_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT21729.1"
                     /translation="MKLLVPVDGSNASISAVKKALELAKKYNFSIKLISVVSAKSSEY
                     RRHENAWKGIDSSVISESEELERQLEMKIKENAQRLLNAIISKLDFSGIRVELEVLLG
                     EPYVKILETARNDNIDMIVMSNRGFSKIKRFFVGSVTQKVISEAPCPVLVVNTGFEP"
     gene            complement(3347..>4471)
                     /gene="lpdA"
                     /locus_tag="PLD67_04080"
     CDS             complement(3347..>4471)
                     /gene="lpdA"
                     /locus_tag="PLD67_04080"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOT21730.1"
                     /translation="RKLVAGVDNLLKNNKVTKIMGEGSFVNKNQLKVKTADGAEIFVD
                     FDYAIIATGSKPVVIPIPGVELPGVVTSDEVLSLEEIPNSMVIIGGGVIGSEFAEVFS
                     AVGCKVTIIEMLPNIIANMDRDIVETLVEKFKKKGVEIYTNTKVVSISKAEKGLSVNT
                     LSSGNEISFNAEKVLLSIGRKPVTDNLALENVGVETNRGAVLVNKNMQTNVPNIYAIG
                     DATAGVMLAHVASAEGIVAVESIMNKKSNIDFKTIPYCVYTKPELAGVGLTEEQAREK
                     GYDVKIGIFPMYINGKAMIEGEQEGLVKYVVDAATGEVLGLHMSGPNATELIVEGALA
                     VRLEATVDEIISTIHAHPTVAESLQEAAHAVHNNAIHIPK"
BASE COUNT         1335 a          885 c          718 g         1533 t
ORIGIN      
        1 aggggtactc tctgtctgaa tcgtccgata tgttatctgc cgaaggaata agattgtatt
       61 taaatgaaga atttaaaaat attcctatca ccgtatataa aacaatagat tcaacaaacg
      121 caaaggctaa gctattagct gttcaaaatg cagagcatgg aacgacaatc attgcagaag
      181 aacaaactaa gggaagaggc cggtttggaa gagatttttt ctcacctgcc gaatcaggta
      241 tttatatgag tataattctt aaggctgaat taagcataga aaattcagtc ttagtgacaa
      301 ctgcagcagc tgtggctgtc tgtcaagcca ttgaaaaatt tacaagtgtt tacccaaaaa
      361 tcaagtgggt aaatgacata tttataggtg ataaaaagat atgcggcata cttacggaag
      421 ctgttaccaa ctttgaaagc ggcatgatgg attgtgtggt agttggaata ggaattaacg
      481 taaaaacaag aaatgaagaa tttcccttgg aattacaaaa tactgcaggc tcaatattta
      541 ttgatgatga caattatata agaaatcagc ttgcagcaga aataattaat aatctgctga
      601 aagcagcaaa taacttggca gacaagagtt ttatgaaatc atataaacag cgctccatgg
      661 tattgggaga aaacatttta tataaaaagg acaataaatg gcatgaaggt tatgcattgg
      721 acattgacga ttatggagga ttagttattt ataccgaaga tggacaaaaa ataacattga
      781 attcaggaga agtttcaatt aaaaagaaat gacaaaccct ggcgagtcat cttgagcgat
      841 agcgaaggac ctcatgataa agacttaata aagaaagaat gagaggaaaa acatgtataa
      901 ggaacaaaca ttcagcgtaa gagacataac acaaatagga atttttaccg cattaacggc
      961 agtaggtgca tttataagca ttcccatagg tcccgtacca ataaccctgc aatcattttt
     1021 tgtactgctg tcaggaatta ttttaggctc taaaaaagct atgttttctc aaataacata
     1081 tttattgttg ggacttatag gcttcccgat tttctccgga ttttcaggag gattgcagca
     1141 tatatttaaa ccaagctttg gttttataat aggatatgtt gctgcagcct atgttgtagg
     1201 taaattaacg gaagaaaaaa ctgcctctca atatttatgg gcggcagttt ttgcgggaac
     1261 cttaattata tatacttttg gacttcccta tatgtattat attttaaata taatgcttaa
     1321 cagcaattta agtatagtta aaattcttca attaggtatg tttgcattta taccgggaga
     1381 tatgctgaaa gctgtaatca tagtattctt tgcccaaaaa atcagccccc ttttaaaggg
     1441 ctgataattt agcttttagt tttgctcccg tgtaatatag attatcccta ggtgttgaat
     1501 aaggaggagc gtatgctaaa tcaagatgca ttaagtcctc tgcctttgca ttaaaagtaa
     1561 tagcagttac caacacatca agccttctgt caactccttc gtaaccgatt aattgggcac
     1621 ctaataatct gcgtgttccc ttttcggcaa cagtcttgat aataattggt cttcctccca
     1681 tatactcagg tttattaggc cttttgtcaa tcgttataac aacattatat cctgatttta
     1741 cagcctcttc ctcagataaa cctgtcatcc ccacggtaag ttcgaaaact ctgtatatgg
     1801 cagtgccaag tattccttta aattcttgtt ctattcccgt aatattgctt ccggcaatcg
     1861 taccggtctt gtttgcagta gagcctaagg gtctgtaaac aggattgccg ttaaaagaat
     1921 aatacatttc gatacaatcg ccgcagctgt aaacatcctc aatatttgtt ttcatatatt
     1981 tatcgacctt gatggcaccg gttgctccaa gttcaactcc catttctttt gcaagggtta
     2041 catttggttt agcacctgta gcaagaagta ccatgtccgc tttcacaatg cttccgtcac
     2101 ctaatacaac cccgctgtca ttaatttcaa ctgtctttgt atttgtataa acattaactc
     2161 ccttttcttt taaatgttct tcaatatata aagataagtc attgtcgatt ccctttgaaa
     2221 ttttagacct ggcaataatc gtaacgtcca accccaatag tttgaagctt tcgcacattt
     2281 ctagacctat gaatccgctt ccgataataa cagctgattt tggtttattt tcatctatga
     2341 aattcttaat atcaatcatg tcatttatat ttcttaaaag aaatacattg tctctatcag
     2401 agccttttaa atcaggctta acagacattg ctccggtggc aattatcagc ttgtcatatc
     2461 tgtccataat ctcgcctgat ttattcttta ctcttaatgt tttgttattc ggatttacag
     2521 acactacttc atgctcagta taaatatcga tattatgttt ttctttagaa aaatccttgt
     2581 ctcttggaac aacgctagaa aaaccttcag cttcattgct tatataataa ggcataccgc
     2641 aacctgcata tgaaatatat ttgtcctttt cataaatgat tatttctgct tccttatcat
     2701 ttcttctgat ttcggttgct gccgaagttc ctgccgcaac ggctccaatt ataacaacac
     2761 gcatattccc tccgctaatt ttataatttc ccattaaggc tcaaaaccgg tgttcacaac
     2821 taaaacaggg cacggtgctt ctgaaataac cttttgagta acagagccga caaaaaatct
     2881 ttttattttc gagaaacctc tgttactcat tactatcata tcgatattat catttcttgc
     2941 tgtttctaat atttttacat agggttctcc caaaagaact tctaattcta ctcttatacc
     3001 actgaaatcc aacttggata ttattgcatt taacaatctt tgtgcatttt ctttgatttt
     3061 catttccaat tgtctttcca gctcttcact ttccgaaatt acggagctgt caattccttt
     3121 ccatgcattt tcatgccttc tgtattcaga actctttgca gaaacaacag aaattagttt
     3181 tattgaaaag ttatattttt ttgccaactc caaagctttt tttactgcac ttattgaagc
     3241 gtttgaacca tctaccggaa caagcagttt cataaatatc actccttata cgttatatta
     3301 attttatatc cacaatttat gaggctaaac atattatatt ctaaatttat tttggtatgt
     3361 gtattgcatt gttatgtact gcatgagctg cttcttggag agattctgct acagtagggt
     3421 gagcatggat ggttgatata atttcatcca ccgttgcttc aagcctaact gcaagagctc
     3481 cttcaactat aagctcggtt gcattcggtc ctgacatgtg aagtcccaaa acttctcccg
     3541 ttgctgcgtc aactacatat tttaccaatc cttcttgctc tccttcaatc attgcctttc
     3601 cgtttatgta cattggaaat ataccgattt ttacatcata tcctttttct cttgcctgct
     3661 cttcagttag tcctactcct gcaagttcag gtttcgtata aacgcagtaa ggaattgttt
     3721 taaaatctat attagacttt ttgttcataa tagattcaac cgcaactatt ccttctgccg
     3781 aagctacatg agcaagcata actcctgctg tagcatctcc gatggcgtaa atgtttggta
     3841 catttgtctg catatttttg tttacaagaa ctgcgcctct gtttgtttca acgccgacat
     3901 tttccaatgc aagattatcg gtaacaggtt ttctgcctat tgaaagcaac accttctcag
     3961 cattgaatga tatctcattc ccgctcgata gtgtatttac gcttagtcct ttttctgctt
     4021 ttgaaattga aaccaccttg gtatttgtat aaatttctac acctttcttt ttaaactttt
     4081 ctactaaggt ttcaacaata tccctatcca tatttgctat aatattaggc agcatttcaa
     4141 ttatcgtaac tttacatccc actgcactga atacttctgc aaattcagaa cctattactc
     4201 ctcctcctat aataaccatg gagtttggga tttcttcaag agacaatact tcatcgcttg
     4261 taacaactcc cggcagttca acccccggta taggaattac gacaggtttt gaacctgtcg
     4321 caattattgc atagtcaaaa tcaacaaata tttcagcacc gtctgcagtc tttactttta
     4381 attgattttt attgacaaag cttccttctc ccattatttt tgttacttta ttgtttttta
     4441 acaggttatc cactccggct actaattttc t
//
LOCUS       DAOSOW010000114         4436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_19729_length_4436_cov_3.186259, whole genome shotgun sequence.
ACCESSION   DAOSOW010000114 DAOSOW010000000
VERSION     DAOSOW010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4436
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19729_length_4436_cov_3.186259"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1208)
                     /locus_tag="PLD67_04085"
     CDS             complement(111..1208)
                     /locus_tag="PLD67_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009847 - spore germination [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HOT21731.1"
                     /translation="MKKLILSLMIISTVFLFGCWDSVEINKREYLFAVGIDKEDDSLK
                     FISEIPKINEGSEEDRLVYTNEGKSFSDFYHLNYLHSDKVISDRLMQVIVLGEELARD
                     SDALKEIFDEIQRSPQMNRRVKICIAKGRAEDIINTEIPSNPIVGRYLSDMLVKLKRE
                     SYQDIFTFDEAILHLGQKGNAMIPVVEINEKSLKIERAAIVKEYELIGFLEPDELEAV
                     MLLLNPANANIRNINIEIDDTNISLGAVDVSMTDNIKLQGEKLQVDYYITLYCYIDSF
                     TIGNNNLEDRNFLNRVKDESIKKISETSTNAVDKLKNSYNADLLQIKDKLYKYHKRDY
                     EKIMDKYDEVFAKADINVYYKMEIKSVGLVK"
     gene            complement(1301..2377)
                     /locus_tag="PLD67_04090"
     CDS             complement(1301..2377)
                     /locus_tag="PLD67_04090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HOT21732.1"
                     /translation="MFNQDITPYQNLSILLLVSFVLQSMLMPVILTGVDGPEGWISIL
                     IAAFILYFSIKTINKAMKKHDEDTIISVSDKLFSKFISKIIGLYYISMFITVNSLIIK
                     DFAEQIKLMMLFRTPISTIIITILLTASYATKKGIQTIANITNIALLTSLIPYLLIIV
                     FSTYYADYTNIFPVYPADAEGIIKSVPNAMLSFLGFSVLLFSNSRVDTKEKNMRINKR
                     YLVISAFLYIFSYILIIVKFGTKEASNLVWPFVSIMKFVNIPGFFFESTEIVGLCLQI
                     IVIFTSICILAFFTNLAMQETFKTNENGYFIFIQIPILYLMAAGLPGMYMMFPYIQVP
                     IYIISGMNLFIPMLVAYAGNRKKT"
     gene            complement(2370..3875)
                     /locus_tag="PLD67_04095"
     CDS             complement(2370..3875)
                     /locus_tag="PLD67_04095"
                     /inference="COORDINATES: protein motif:HMM:NF015292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HOT21733.1"
                     /translation="MKDIDLTQKIDLDLNKNEQILRKIFENTDDIIFRKLEGGKIKKL
                     KMLLFYVDGMTSKEAISEYAISSLLEGLDLEKIQNMSNVQLQDAIVRTSISISEIQIL
                     TTIEECVDKILSGETVLLLENCNKGIMLSSRGWPMRGVQEPSAETLIRGARDGFNETM
                     KVNISLIRRRIRDPKLKVKYTQVGNRSKTDIAILYIEDLVNNTVLETVEKRINNIEID
                     AVLESSYIEEMIEDDGYSPFPQIENTERPDAAASALLEGRVVIAIDNTPSVLIAPAIF
                     VSFMQSSEDYYERWLPACATRILRYFALPIVMLLPALYVAVTQYHPNLLPTQLALYVA
                     ASRANVPFPPYFEALLMELVIELVREASFRITTPVGSTIGLVGGLVIGQSSVEAGLIT
                     PLAVIVVALTAIASFTIPSYNFGATLRLIRFGFIILAATFGLFGISIGLSFLIIHLCT
                     LKSCGIPYMTPFTRFVENKRDLKDTIVRPRISKMVRKPDYLHMEKKERKNV"
     gene            complement(3884..4084)
                     /locus_tag="PLD67_04100"
     CDS             complement(3884..4084)
                     /locus_tag="PLD67_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21734.1"
                     /translation="MFFINDLKRIINNDVIIVFLIISYILIFVTSKELKRNNYHRDYK
                     IVRFTGIIYGLLAIAAATAIYW"
     gene            complement(4233..>4436)
                     /locus_tag="PLD67_04105"
     CDS             complement(4233..>4436)
                     /locus_tag="PLD67_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT21735.1"
                     /translation="ILPDSIELTELSELFITGSKTLKEKMDEYEKEIIESTLAVSKNM
                     KEASMRLGIDISTLVRKKQKYNL"
BASE COUNT         1391 a          847 c          637 g         1561 t
ORIGIN      
        1 tatttttgac aaactcgcat aaacttgtgc cgaataatat gcaattacta ttgataaaac
       61 aacgaataca gctgaaacaa aaactttctt tgaatatttc atagtacctc ctatttaact
      121 aatccaacac tttttatttc cattttgtaa tagacgttta tatctgcctt tgcaaaaact
      181 tcatcatatt tatccattat tttttcataa tcccttttgt gatacttata aagtttatcc
      241 ttaatctgca gtaaatccgc attgtatgaa tttttgagct tatccacagc atttgttgaa
      301 gtttcggaaa ttttcttaat ggattcatct ttcaccctat tcagaaaatt cctgtcttca
      361 aggttgttgt tgccgattgt gaaagaatca atgtaacagt aaagagtaat ataataatct
      421 acttgaagct tttcaccttg taattttata ttgtctgtca tacttacatc aacagcaccc
      481 aaggatatgt ttgtgtcatc tatctcaatg tttatattcc tgatattggc atttgccggg
      541 tttaaaagaa gcattactgc ttccaattca tccggttcca aaaaaccgat taactcatat
      601 tccttaacta tggctgctct ttctattttc aaactttttt catttatttc taccacaggt
      661 atcattgcat tacctttttg cccaagatgg agaatagcct catcaaaagt aaatatatct
      721 tgataacttt ctctttttaa tttaacaagc atatcgctca gatatcttcc aacaatggga
      781 ttgctgggta tttcagtatt gattatgtct tcagccctcc cctttgctat acaaattttt
      841 actctgcggt tcatttgggg agagcgttgg atttcatcaa aaatttcttt taatgcatct
      901 gaatctcttg ccagctcttc ccccagcact attacctgca taagtctgtc ggaaattact
      961 ttatccgaat gaaggtagtt tagatggtaa aaatctgaaa agcttttgcc ttcatttgta
     1021 taaaccagcc tatcttcttc gcttccttca tttatttttg gtatttccga aataaatttt
     1081 aaactatcat cctctttatc aatacccact gcaaaaagat attctctctt attgatttca
     1141 acactgtccc agcatccgaa cagaaaaaca gttgatatta tcattaaact cagtattagt
     1201 tttttcatat agacctctta tataaacgat atactgttat aaaattttct cgttgtcatg
     1261 agattcttca ctgcattaat aatgacacga ttattcctaa ctatgtcttt tttctattac
     1321 ctgcatatgc caccagcatc ggtataaaaa gattcatacc gcttattatg tatataggta
     1381 cttgaatata cggaaacatc atgtacattc ccggcagtcc tgcagccatt aagtacaaaa
     1441 taggtatctg aatgaatatg aaataaccat tttcatttgt tttaaatgtt tcctgcatag
     1501 caagatttgt aaaaaatgca agtatgcata tgcttgtaaa aataacaatt atttgtaggc
     1561 ataaacccac aatttccgta ctttcaaaga agaagcccgg aatatttaca aatttcataa
     1621 tagaaacaaa aggccaaaca aggttggaag cttcctttgt tccaaacttt acaataatca
     1681 atatatagct aaaaatgtac aagaaggcgc ttataacaag atacctctta tttattctca
     1741 tgtttttttc cttggtatct accctgctgt tagaaaataa aagtacagaa aatccaagaa
     1801 aactcaacat ggcatttggt actgatttaa taattccttc agcatccgca ggatataccg
     1861 ggaaaatatt tgtatagtct gcataatatg ttgaaaaaac tattatcagc aaatatggta
     1921 taagcgatgt taaaagagca atatttgtta tattggcaat agtttgtata ccttttttag
     1981 ttgcataaga tgcagtcaat aaaattgtta taataatagt gcttatagga gttctaaaca
     2041 acatcatcaa tttaatctgc tcggcaaaat cctttattat cagactattg accgttataa
     2101 acatcgatat gtaatagagt ccgataattt tagaaataaa tttagaaaaa agtttatccg
     2161 aaacacttat aatcgtatct tcatcatgtt ttttcattgc tttattaatt gtctttattg
     2221 aaaaataaag tataaacgcc gcaattaata tcgaaatcca tccttcggga ccgtcaactc
     2281 ccgtaagtat tacaggcatt aacatagact gcaatacaaa tgataccaat agtaaaatcg
     2341 ataaattctg ataaggtgta atatcttgat taaacatttt tcctctcttt tttctccata
     2401 tgcagataat ccggttttct gaccattttg ctaattcttg gtcttacaat cgtatccttt
     2461 aagtccctct tattttcaac aaatcttgta aatggtgtca tgtaaggtat tccgcagctt
     2521 ttcaatgtgc aaagatggat tatgagaaag cttaagccta tggatattcc aaacaacccg
     2581 aatgttgctg ccaatattat gaatccgaat cttataagcc ttaatgttgc accaaaatta
     2641 tagcttggta ttgtaaagga ggctattgca gtcaatgcaa caacaattac agccaaaggt
     2701 gtaatcaagc ctgcttctac cgaagattga ccaataacca agcctcccac cagacctatg
     2761 gttgagccga caggagttgt aattctaaag gatgcctccc ttaccagttc aattacaagc
     2821 tccatcaata atgcttcaaa gtaaggagga aacggaacat ttgctcttga agctgctaca
     2881 tacagagcaa gctgagtagg aaggaggttg ggatggtatt gggtaacagc cacatacaaa
     2941 gcaggcaaca gcataacaat gggcagggca aaatacctta atattcgagt tgcacaggcc
     3001 ggcagccacc tttcataata gtcttccgaa gactgcatga atgaaacaaa aattgcggga
     3061 gcaatcaaga cggaaggagt attatctatt gctattacaa ctcttccttc caatagtgca
     3121 gatgcagcag catcaggtct ttcagtattt tcaatttgag gaaaaggcga atagccgtca
     3181 tcttctatca tttcttcaat gtatgaactt tcaagaacgg catctatttc aatattgttg
     3241 attctttttt caactgtttc caatactgta ttgtttacca aatcttctat gtacaatata
     3301 gctatatctg tttttgagcg atttcctacc tgagtatatt taacctttaa cttagggtct
     3361 cttattcgtc ttctgattaa tgaaatgttc accttcattg tttcattaaa tccgtctctt
     3421 gcacccctta tgagagtttc agcggaaggc tcctgtacac ctctcatcgg ccagcctctc
     3481 gacgaaagca ttatcccctt gttgcagttt tccaataata gtacggtttc accggaaaga
     3541 attttatcaa cacattcctc aatggttgtc agtatttgaa tttccgaaat tgaaatgctt
     3601 gttcttacaa ttgcatcctg caattgaaca tttgacatat tttgaatttt ttcaaggtca
     3661 agaccctcaa gaagagagct tattgcatac tcgcttattg cttccttgga agtcattcca
     3721 tccacataaa acaaaagcat tttaagcttt ttaatcttgc ccccttctaa ttttctgaat
     3781 attatgtcat cggtattctc aaaaatcttt cttaaaattt gctcattttt atttaaatcc
     3841 aaatctattt tttgagttaa atcaatatcc ttcataatct cccctaccag tatattgctg
     3901 tggcagctgc aatagctaaa agtccgtata ttattcccgt aaaacgcact attttatagt
     3961 ctctgtggta attgtttctt tttaattcct tgcttgttac aaatattaat atatagctta
     4021 taattaaaaa gactattatt acatcgttgt ttattattct ttttaaatca ttaataaaaa
     4081 acatggaccc tccttaatgg gataagctgt tagtaatatc tgcatttttt aagtttttat
     4141 accataaaat gaaaaaacat tataaataca acaaaaagac tcctttatgt taataaaaga
     4201 gccttatgac agtacccccg tccccttgtc atctataggt tgtatttttg cttttttctg
     4261 accaatgttg aaatatctat acctaatctc atcgatgctt ctttcatatt ctttgaaaca
     4321 gctaaggtgg attcaataat ttctttttca tattcatcca ttttttcttt cagtgttttt
     4381 gagcccgtta taaatagctc agaaagctca gtcagctcta tgctatcagg caaaat
//
LOCUS       DAOSOW010000115         4397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_19938_length_4397_cov_3.419392, whole genome shotgun sequence.
ACCESSION   DAOSOW010000115 DAOSOW010000000
VERSION     DAOSOW010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19938_length_4397_cov_3.419392"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLD67_04110"
     CDS             complement(<1..385)
                     /locus_tag="PLD67_04110"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT21736.1"
                     /translation="MEEIIWLENIKIAFKSILSNKMRSLLTMLGIIIGISSVISIVSI
                     GDSMKGVMDDIYKNFGKNRAVLYVGNIEDFRTTDFFYNDDLEILKERFSEKLAYICPS
                     DSLRSDVTAGRNTIKLNMEFIDSGYD"
     gene            complement(363..1070)
                     /locus_tag="PLD67_04115"
     CDS             complement(363..1070)
                     /locus_tag="PLD67_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21737.1"
                     /translation="MEKMIRIENLNKIYKNGSVEVHALNDVNMEVSRGEFLAVMGHSG
                     SGKSTLMNIIGCLDRPSSGKYFLDGIEIDKQSPDELSLIRNKKIGFVFQAFNLIPRTN
                     VLSNVELPMIYAKVKSKQRSERALELLEKVGLKDRINHMPNELSGGQMQRAAIARALA
                     NNPPIILADEPTGNLDTNSSEEIMKIFKDLNREGTSIIVVTHEPEVAKYADRIIVFKD
                     GCIIEDRWKEGGRNYLA"
     gene            complement(1077..2306)
                     /locus_tag="PLD67_04120"
     CDS             complement(1077..2306)
                     /locus_tag="PLD67_04120"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT21738.1"
                     /translation="MMKRFFTRKKVIVFVVVAALAAIIFWLVNKGSKAPEVTANVSKL
                     ERKTLEHVISVKAPLVGIEKAEVTSPLNYKINDIRVKEGDLVKKDDILAVLDSEDLEK
                     EIEYEENQIELSKMEAEEKIKALQIEYDKALASYRDMETVYEQNKDLYENGVITEEAF
                     KKTEKDLVVLKKSLEGYNAVNGKIQLSQADKKRIEIQEDRLKLKKEELEKVMIRSSID
                     GTVTRVNVNIGRYAKDTENSQAMFVVENLEKLQMKAAVSEFDVGKLKLNQEVEIYSDI
                     LGKGFAKGKVARISPTAEQKDNNTMERVIPVLIDVTERPENLIAGVIATAKIRTEKKE
                     NVFAVPSGAIVQDGEEYKIYILNEDNTVRSIPVEIGLETDLESEITGDLYEEMKYVIN
                     PDETFEDGMTVIPNEIE"
     gene            complement(2404..3126)
                     /locus_tag="PLD67_04125"
     CDS             complement(2404..3126)
                     /locus_tag="PLD67_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21739.1"
                     /translation="MKRLLIPLAVIMAGAVCVLIRQPESYAAGKISIEQNTYGLYEKS
                     NYLYIKSGKKYVKIIDEEIIDFKTYDIDSDKDDEILAISKHISEEYGSDFIIYDIYEA
                     DGIILTKEIYRQDFFDFKPWKVDACNLDNDGETDIFIGVYKDTVFYKDLRKRPFFYSW
                     DGDRLHKKWLGSFFSDWDLVDIAFGDFYNLGYDVAAVLEKNKDACRIGIYQFIGFGFE
                     NMKTINSVDNSFRSQTELLFGK"
     gene            complement(3128..>4397)
                     /locus_tag="PLD67_04130"
     CDS             complement(3128..>4397)
                     /locus_tag="PLD67_04130"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HOT21740.1"
                     /translation="GEALLLPEPKIQHKYSSVSTPAGRQFRFMGQRYSFDAEVMQALI
                     QPIIRPIPSGLDVIASFGSKRAEELLDTYYKPKEDWDKYEENLNLMRKKQTEITDDEW
                     KSDLYKGWLWSIKSSAVSFEDKEGMPHFMRNEKWTDKNIHTALGSYAELKHDSILYMK
                     QSGAEMGGGPEPIIPYNYVEPNVEVYAKLKWLAENTKAQLQERNMLKDEIGLVLDQII
                     DIQDTLMNVSVKELTNQDITDEENLKLYRYGGLIDSVIQIMQMNLMRNDVDTSNDFTT
                     ALIADVSTIAPNDLFPKGTYLEIGNGLPCEIYVVCQTNGKTYLARGALFNYYEFLSDK
                     RLTNHEWQTLVGVKRMAMVYDEEKNIHVPMDIYDEDGNRILEEDEYDFENIMIIGPSE
                     NMVPKPAWTESFISQEENKVTIKDISISWE"
BASE COUNT         1153 a         1003 c          669 g         1572 t
ORIGIN      
        1 tatcgtatcc ggagtcaatg aattccatat tgagttttat ggtattcctg cctgccgtaa
       61 catcgcttct caaggaatcg gaagggcaaa tataagccaa cttttcactg aatctttcct
      121 ttaaaatttc taaatcatcg ttataaaaaa agtccgtggt tctgaaatcc tctatgtttc
      181 ccacatacaa aacagccctg tttttgccga aattcttgta tatatcgtcc attaccccct
      241 tcatgctgtc gcctatggac acgattgaaa taaccgagct tattcctatg ataattccca
      301 gcatggtgag aagagaccgc attttgtttg ataaaataga tttaaaagct atcttaatat
      361 tttcaagcca aataatttct tccaccctct ttccatctgt cttcgataat gcatccgtcc
      421 ttaaacacaa taattctgtc tgcatatttt gccacttcag gttcatgggt gaccactatg
      481 atgctggttc cttccctgtt gaggtctttg aatattttca tgatttcttc agaagagttg
      541 gtgtctaaat tccccgtagg ttcatcggca agaattattg gaggattgtt tgccaaggct
      601 ctggctatgg cagctctttg catttgaccg cctgataatt catttggcat atggtttatc
      661 ctatccttca agcctacctt ctctaaaagc tccaaagccc tttcacttct ttgttttgac
      721 ttgactttgg catatatcat tggaagttcc acattgctaa gcacattggt cctggggatt
      781 aaattaaatg cctgaaaaac aaaaccgatt tttttattcc ttatcaaaga aagctcatcg
      841 gggctttgtt tatcaatttc aattccatca agaaaatact tgccgctgct tggtctgtca
      901 agacaaccta taatattcat gagggttgac tttcctgagc cggaatgccc catgacagcc
      961 aggaattctc cccgggagac ctccatattc acatcattta aggcatgaac ttccacggag
     1021 ccgtttttgt agattttatt caagttttct atcctaatca tcttttccat atcaacctac
     1081 tcaatttcat taggtattac tgtcataccg tcttcgaatg tctcgtcggg atttatcaca
     1141 tatttcattt cctcgtataa atcacccgtg atttctgatt ccaaatcggt ttcaagacca
     1201 atttcaaccg gtatggacct tacggtattg tcttcattca atatgtaaat cttgtattcc
     1261 tccccgtctt gcactatggc tcccgatggg accgcaaata cattttcttt cttttcggtt
     1321 cttattttag ctgtggcaat tactcctgct attaaatttt ccggcctttc cgtaacatct
     1381 attaaaacag gaatcactct ttccatggtg ttattatctt tttgctctgc tgtaggagat
     1441 attctcgcaa cttttccttt tgcaaatccc ttacccaaaa tatcagaata aatttctact
     1501 tcctgattca atttaagttt tcccacgtca aattcgctga ctgctgcctt catttgcagc
     1561 ttttccagat tttccacgac aaacattgcc tggctgtttt cggtatcctt ggcatatctt
     1621 ccgatattga catttaccct cgtgacggtc ccgtcaatag aacttcttat catgaccttt
     1681 tcaagttctt ctttctttaa cttaagccgg tcttcctgta tttcaatgcg ctttttatct
     1741 gcttgggaca attgtatttt cccattgacg gcattataac cttctaggct tttctttaaa
     1801 acaaccagat ccttttctgt ctttttaaag gcttcttcgg ttattacgcc gttttcgtac
     1861 aggtccttgt tttgctcata tactgtttcc atatccctgt agcttgccaa agccttgtcg
     1921 tattcaatct gcagggcctt gattttctcc tctgcttcca ttttgcttaa ttcaatttga
     1981 ttttcttcat attctatttc tttttccagg tcctcgctgt ccaaaacggc taaaatatcg
     2041 tcctttttaa ccaaatctcc ttccttcacc cttatatcgt ttattttgta atttaaagga
     2101 gatgtaacct ccgccttttc aatccctacc aggggtgctt tcaccgaaat aacatgctct
     2161 aaggtttttc tttccaattt tgaaacattt gccgtcactt ccggcgcctt ggaacccttg
     2221 tttacaagcc aaaatatgat tgcagcaaga gccgcaacca caacaaaaac gataaccttc
     2281 ttacgtgtaa aaaacctttt catcatccct ccaaattgca gatcgactta attttagtat
     2341 ctattataat ttcataaaat tatgtaatcc ttaaagaact attaaaaatt aattaataac
     2401 cctctatttg ccaaacagca gctctgtttg agaacgaaaa gaattgtcca cggaatttat
     2461 agtcttcatg ttttcaaagc caaatcctat gaattggtaa atgcctattc tacaggcatc
     2521 cttgtttttt tccaaaaccg cagccacatc atagcctaaa ttataaaaat cgccaaaagc
     2581 tatgtccacc aaatcccagt cggagaaaaa tgagccaagc cacttcttat gcaagcggtc
     2641 tccgtcccaa gaataaaaga atggcctctt tcttagatcc ttatagaaaa cagtgtcttt
     2701 atataccccg ataaatatat ccgtttcacc gtcattatcc aaattacagg catcaacctt
     2761 ccaaggtttg aaatcgaaaa aatcctgtct gtaaatttct tttgtcaata tgataccgtc
     2821 agcttcatat atgtcatata tgatgaaatc actgccgtat tcttcagaaa tatgtttaga
     2881 tatagccaaa atctcatcat ctttgtcaga gtcaatgtca tatgttttaa aatcaattat
     2941 ttcttcatca atgattttaa catatttctt gccacttttt atatataaat aattgctttt
     3001 ttcatataaa ccataggtat tctgttctat cgatattttt ccggcggcat aagattcagg
     3061 ctgtctaatg agtacacaga cagcccctgc cattatgacc gctagcggta ttaatagtct
     3121 cttcatttta ctcccagcta atacttatat ccttaatggt taccttgttt tcttcctgtg
     3181 atataaatga ctctgtccat gccggcttag gaaccatgtt ttctgaagga ccgataatca
     3241 ttatattctc aaaatcatat tcatcttctt cgagaatcct gtttccgtct tcgtcgtata
     3301 tatccatggg gacatgaata ttcttttcct cgtcataaac catagccatt ctctttaccc
     3361 ccaccaaggt ctgccattcg tgattagtaa gtctcttgtc ggaaagaaat tcataatagt
     3421 taaacaaggc tccccttgct aaataagttt ttccgttggt ctgacaaact acatagattt
     3481 cacagggtag gccgtttcct atctccaaat acgttccttt cggaaagagg tcgtttggcg
     3541 caattgttga tacatctgct attaatgccg tagtgaaatc attgcttgta tcaacgtcat
     3601 ttctcataag attcatttgc attatttgaa ttacactgtc aatcaatcca ccgtatctgt
     3661 aaagttttaa attctcttca tctgtaatat cttgatttgt tagctcttta actgaaacat
     3721 tcattaaagt atcttgaata tcaattattt ggtctaatac cagaccgatt tcatccttaa
     3781 gcatatttct ttcttgtagc tgtgctttcg tattttctgc aagccactta agctttgcat
     3841 atacttccac attcggctcc acataattat aaggtattat tggctcaggt cctccgccca
     3901 tttcggcacc ggactgcttc atgtacaaga tgctgtcatg ctttagttcg gcatagctgc
     3961 caagagctgt atgaatgttt ttgtccgtcc atttttcatt tctcatgaaa tggggcatac
     4021 cttctttatc ctcgaaggat acggcagatg atttaatgct ccacagccat cctttatata
     4081 aatctgattt ccattcatca tctgtaattt cagtctgttt ttttcgcatg aggtttaaat
     4141 tttcttcgta tttatcccaa tcttcctttg gtttgtagta tgtatccaaa agctcttcgg
     4201 ctcttttgct gccaaaggat gcaatcacat caagacctga aggaatcggt cttattattg
     4261 gctggattag tgcctgcatt acttccgcat caaaggaata tctttgtccc ataaacctga
     4321 actgccttcc cgcaggtgta ctgactgaag aatacttatg ctgaattttt ggttcaggca
     4381 aaagaagtgc ttcccct
//
LOCUS       DAOSOW010000116         4355 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_20188_length_4355_cov_3.830698, whole genome shotgun sequence.
ACCESSION   DAOSOW010000116 DAOSOW010000000
VERSION     DAOSOW010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20188_length_4355_cov_3.830698"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLD67_04135"
     CDS             <1..782
                     /locus_tag="PLD67_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT21741.1"
                     /translation="FIRWLFASVILLIVGQKIEKPDWKEVCKQWPKITMLSMTGIIGY
                     NIILYYALYYTSPLNAALVNSINPSFIAIVSAFYLKERITKYHVFGIMISFLGVWIVL
                     TKGNLLQAFNMKYNRGDLLVIAAVITWTSYSVISKKLTTVPPITSTGVSGLMAGIILF
                     PFAMTSGVNYLNMSTLALTAVVYIILFPSLLSFVFWNIGVREIGAGKTGIFLNLNPVF
                     TAFISRAMGKDITWVQVIGGLLVFTGVYITTAFKGKTARLK"
     gene            793..1308
                     /gene="cobO"
                     /locus_tag="PLD67_04140"
     CDS             793..1308
                     /gene="cobO"
                     /locus_tag="PLD67_04140"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOT21742.1"
                     /translation="MEKGYIHIYTGNGKGKTTAAFGLAVRAALSGKKVFIGQFVKNME
                     YNETKIKDYLSNIEIKQLGRGCFIYEDPEEIDKETAKKGLEECSTLLASGEYGLIILD
                     EINIALYFKLLEVSDVVSALKNKAENTEVVLTGRYAPKELIEIADLVTEMVEIKHYYS
                     RGVLSRDGIDH"
     gene            complement(1361..2164)
                     /locus_tag="PLD67_04145"
     CDS             complement(1361..2164)
                     /locus_tag="PLD67_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012671940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOT21743.1"
                     /translation="MKKSEIKPEEGKKPSPKSPKYTEVNSKFIDKWIDEGWEWGQPVS
                     HEVFERAKNNDWFVLLTPTKPVPKEWFCEMKNAEVLGLASGGGQQMPIFTALGAKCTV
                     LDYSEKQIASEIEVAKRENYEIKTIRADMTKPLPFEDETFDLIFHPVSNCYIEDVLPV
                     WKECFRVLKKGGILLAGLDNGINFIFDDDETTPVRKLPFNPLKDEELYQLSVEKDWGI
                     QFSHTIEEQIGGQIKAGFIIEDIYEDTNGSGRLHELNMPTFYATKAVKK"
     gene            2372..2746
                     /locus_tag="PLD67_04150"
     CDS             2372..2746
                     /locus_tag="PLD67_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT21744.1"
                     /translation="MNIIISNSSGQPIYEQIASQIKSMIITGELREGEALPSMRLLAK
                     ELRISVITTKRAYDELEREGFIVSFTGKGSFVAGKNIELIKEQHLKEIENHMQEILKL
                     SKECGLKLDEMIEMMTLLYEGE"
     gene            2748..3596
                     /locus_tag="PLD67_04155"
     CDS             2748..3596
                     /locus_tag="PLD67_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019242167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21745.1"
                     /translation="MEYSLKLENVTKVYKDFKLDNINITLPKGSIMGFIGENGAGKTT
                     TIKLILDLISRDGGNISILGKDNKTEMDSVKENIGVVLDESSFPENLNAREINNMLKK
                     VYKTWDEGKFSKFLDMFSLPYNKSIKDYSRGMKMKLSTAVSMSHDSKILILDEATSGL
                     DPVVREEILDTFLDFLQDETNSIFVSSHIISDLEKICDYITFIHKGKIIFSETKDDLL
                     DNYGILKCSPEEYENIDKSLVKGMRKNKFGIEALVLKDRITGPYLIDRASLEDIMIFI
                     IKEQVQ"
     gene            3593..4204
                     /locus_tag="PLD67_04160"
     CDS             3593..4204
                     /locus_tag="PLD67_04160"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HOT21746.1"
                     /translation="MTGLILKDLLNLKKQGKVYLILIIFYFAIGLVNENSSMFASMMT
                     MVAVLIPITAMAYDERCKWDRYALTMPVSRKDMVMSKYLLGLIFLTAAFVISILLNMV
                     FSNMPINEKILTALGNFFAGLTLMAVIFPLLFKFGVEKGRILMMIVIFIPVAAVMMIS
                     KIGIPMPAEEKLEQLIYFLPIIGVLLFIISIYVSLRIYRKKEF"
BASE COUNT         1441 a          713 c          870 g         1331 t
ORIGIN      
        1 catttataag atggctgttt gcatcggtta ttttattgat agttgggcaa aaaatagaaa
       61 agcctgattg gaaggaggtt tgcaaacagt ggcctaaaat tacaatgctg tctatgaccg
      121 gcattatagg ttataatata attttgtact atgctctata ttacacatcg ccgcttaatg
      181 ccgccttggt taattctata aatccaagtt ttatagcaat agtttcagca ttttatttaa
      241 aagaaagaat cacaaaatat cacgtttttg gaataatgat ttcattcttg ggtgtatgga
      301 ttgtattaac caagggaaat ttgctccaag ctttcaatat gaagtataac agaggagatt
      361 tgcttgtaat agcagctgtg attacatgga catcatattc tgtaataagt aagaagctta
      421 caactgttcc ccccattact tctacaggtg tttcgggact tatggctgga attatactat
      481 ttccgtttgc aatgaccagc ggagttaatt atttaaatat gagtaccttg gcattgacag
      541 ccgtagttta tattattttg ttcccgtcat tattatcctt cgtcttctgg aatattggag
      601 tgagggaaat cggtgcaggc aagacaggca tatttttgaa tttaaaccct gtgtttactg
      661 catttatcag cagggcaatg gggaaggata ttacatgggt gcaggttata gggggactat
      721 tagtgtttac gggagtttat ataactactg catttaaggg taagactgca aggctcaaat
      781 gaggaggtta taatggagaa aggttatatt catatatata caggtaacgg aaaaggaaaa
      841 acaactgctg cctttggtct tgctgtaagg gctgctttat ccggcaaaaa agtttttata
      901 gggcagtttg tgaaaaacat ggagtacaat gaaacaaaga taaaagatta tttaagcaat
      961 atcgaaataa aacaattggg aaggggatgc ttcatatacg aagatcctga agaaattgac
     1021 aaagaaacag caaaaaaagg cttggaagaa tgcagcactc ttctggcaag cggagaatat
     1081 ggactgataa tccttgatga aataaacata gccctttatt ttaagctgct tgaagtatct
     1141 gatgttgtaa gtgcattaaa aaataaggcc gagaacactg aagttgtgtt gacaggtcga
     1201 tatgctccca aggagcttat tgaaatagcc gatttagtaa ccgagatggt tgaaataaag
     1261 cactattaca gccgcggagt attatccaga gacggtatag atcactaaaa ttattaggga
     1321 cggttctttt tagtaagaac cgtcccttaa aaatgaaatg tcattttttt actgcctttg
     1381 ttgcataaaa ggtaggcata ttcaattcat ggagccttcc tgaaccgttt gtatcttcat
     1441 aaatgtcttc tataataaat ccggctttta tttgtccccc tatttgttcc tcaatagtat
     1501 gagaaaattg aataccccaa tccttttcta ccgaaagctg ataaagctct tcatccttca
     1561 gcggattaaa gggcagcttg cgtacaggtg ttgtttcatc atcatcgaaa ataaaattaa
     1621 ttccgttatc taatcctgcc aataatattc ctcctttttt caacactctg aagcattcct
     1681 tccaaacagg aagaacatcc tctatatagc agttagatac aggatgaaat atcaaatcaa
     1741 aagtttcatc ctcaaagggc aagggctttg tcatatcagc ccttatagtt ttaatctcat
     1801 aattttccct tttagcaacc tcaatttcac ttgcaatttg tttctctgaa taatcaagta
     1861 cagtacattt tgcacccaat gcagtgaaaa ttggcatctg ctgaccgccg cccgaagcaa
     1921 gaccaagaac ttcggcattc ttcatctcgc aaaaccattc ctttggtaca ggcttagtcg
     1981 gtgttaacaa aacaaaccaa tcattgtttt ttgctctttc aaatacttcg tgactgacag
     2041 gctgccccca ttcccatcct tcatcaatcc atttatcaat gaattttgaa ttaacttctg
     2101 tatactttgg agattttgga gacggttttt ttccttcttc gggttttatc tctgattttt
     2161 tcataaaact atcctttcag ttttatatat cgttgataca atattatcat ttatattatg
     2221 aaaatcaata taatttttat atgacaaggg gacgggggta ttgtcagctt tttgttttga
     2281 gagcccttag ttaaaaaaat tccaaaaaaa tatattgaca aagttgatat attgtatata
     2341 atggatatat acactattta cggagatgga aatgaatata ataataagca attcaagcgg
     2401 tcaaccaatt tacgaacaga ttgcttctca gataaagtct atgattatca caggtgaatt
     2461 aagagaggga gaggcactgc cttccatgag acttcttgca aaggagctga gaatcagtgt
     2521 tattacaaca aaacgggcat acgatgaact tgaaagggaa ggattcattg tttccttcac
     2581 cgggaaagga agttttgtgg caggcaaaaa tatcgaattg ataaaggaac agcatcttaa
     2641 ggagatagaa aaccacatgc aggaaatact taaattatca aaggaatgcg gtttgaagct
     2701 ggatgaaatg attgaaatga tgacattgct ttatgaagga gaataaaatg gaatactcat
     2761 tgaaactgga aaatgtaaca aaggtctaca aggattttaa gttagacaat atcaacatta
     2821 ccctgcccaa gggaagtata atgggtttta taggggaaaa cggagccgga aaaacaacca
     2881 ccataaaact gattttggat ttaatcagcc gggatggggg aaatatttca attttaggca
     2941 aagataacaa aactgaaatg gattccgtta aggaaaacat cggagtcgtt ttggatgaat
     3001 ctagtttccc tgaaaatcta aacgcaagag aaataaacaa catgttaaaa aaggtatata
     3061 agacctggga cgaaggcaaa ttttcaaaat tcctcgatat gttctcactc ccctataata
     3121 aaagcatcaa ggattactca agaggaatga aaatgaagct gtcaacggca gtttccatgt
     3181 ctcatgattc aaaaatatta attttggatg aagccacgag cggccttgac cctgttgtca
     3241 gggaagaaat cctggatacc tttttggact ttcttcagga tgaaacaaat tccatctttg
     3301 tatcgtccca cataataagt gatttggaaa aaatctgcga ctacatcacc tttattcaca
     3361 aggggaaaat tatattctcg gaaaccaagg acgatttgct ggacaattac ggtatcttaa
     3421 aatgctctcc ggaagagtat gaaaacattg ataagagcct tgtaaaggga atgaggaaaa
     3481 acaaattcgg aatcgaagct ttggtgctta aggataggat cacgggacct tatctgattg
     3541 acagggcttc cctggaagat ataatgatat ttattataaa ggagcaagtg caatgactgg
     3601 attgatttta aaggatttgt tgaacttaaa gaaacaaggg aaggtgtact taatattaat
     3661 aatattctat tttgccatag gtctggtcaa tgaaaactca tccatgttcg cctccatgat
     3721 gactatggtg gccgtattga tacctattac ggcaatggct tatgatgaaa ggtgcaaatg
     3781 ggacagatat gcccttacaa tgccggtatc aagaaaagac atggttatga gcaagtactt
     3841 gctgggcttg atatttttga ccgctgcttt tgttatatca attcttctta acatggtttt
     3901 ttccaatatg cccataaatg aaaaaatctt gacggccttg ggcaactttt ttgccggatt
     3961 gacattgatg gcagtcatct ttcccctcct cttcaagttc ggagtggaaa aagggcgcat
     4021 cctgatgatg attgttatat tcataccggt ggcagctgtg atgatgattt caaaaattgg
     4081 aatcccaatg cccgcagagg aaaaattaga acaattgatt tattttttac ccataattgg
     4141 tgtactctta tttataattt cgatttatgt ttctttacgg atatacagga aaaaagagtt
     4201 ttgatgctta ctaatgggag acaggaaaag attaaaagga aggaaagaat atgaaaaaaa
     4261 ttgatacggt aaatatttga gatccttgag tttttgggag aagaactcgc atcggaagca
     4321 atctggtaat atgtcaagat aaaaaaacaa ataaa
//
LOCUS       DAOSOW010000117         4325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_20388_length_4325_cov_2.981265, whole genome shotgun sequence.
ACCESSION   DAOSOW010000117 DAOSOW010000000
VERSION     DAOSOW010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20388_length_4325_cov_2.981265"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..980
                     /locus_tag="PLD67_04165"
     CDS             216..980
                     /locus_tag="PLD67_04165"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT21747.1"
                     /translation="MDNLERIAQFEKFSPMLYLDYKLTSWGRMLCDIGNKESYTSNYA
                     VIYRNENVDNIIKEVEEYYNSRSIIPKIFNRCGSVELDILRPFFTKHDYAIREFDMDL
                     MILDTHSTRNKIAEKVEVKIINRALEGQEYNLAIEQDNGETYGVKLLNKQIASGNNMF
                     FAYNELKKPVSMALAEKYNDVVYITDVYTTPSQRCNGYGTAVVYAVLSHYHNSLIYLH
                     TDNPDAASIYRRLGFSGKRLKSWWAVKGSLPEWCLE"
     gene            968..1093
                     /locus_tag="PLD67_04170"
     CDS             968..1093
                     /locus_tag="PLD67_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21748.1"
                     /translation="MFGIISTESRYEFVGDSMEKLQDDYNLITGVYIGDVLDVHL"
     gene            1134..1766
                     /locus_tag="PLD67_04175"
     CDS             1134..1766
                     /locus_tag="PLD67_04175"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012294293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT21749.1"
                     /translation="MLDSKGFDLWAHGYDKSVNLSEENNEYPFAGYKDVLNYIYNQVR
                     KHNYSSVLDIGFGTGILTTQLYNEGYHITGIDFSRTMIDIAKSEMPEAILINWDFTRG
                     LPVEIKNNKYDYIISTYAIHHLPDEEKIKFIKSLLKLLNVNGKILIGDVSFMTRKELE
                     ECKETHTEIWDDEEFYFVANEMMMSLNEFYCNYEKISYCSGILIVADYLL"
     gene            1839..2870
                     /locus_tag="PLD67_04180"
     CDS             1839..2870
                     /locus_tag="PLD67_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOT21750.1"
                     /translation="MLKKPNRLNRGDKIALVSLSWGGAGDENLLWRYKQGKERLEQVF
                     GLEVVEMKHTLAGTEYIYNHPEKRAEDLMNAFQDKSIKGIIACIGGIDSIRMLPFIDF
                     DIISNNPKIFMGYSDTTTTHLFCYKAGISSIYGPTLLVDFAENVSMSPYTVEHIEKTL
                     FKSEAIGEIEPSPKWTSEYLPWEGKNKLIQRKFKDNSGYELLQGKGITKGRLIGGCIE
                     VFDSLRGTEVFPKLEDFDDSILFLETSEEKPPAWIIECSLRIYGINGILDRIKGMIWG
                     KPQDETYYAEYKEAIKKVMKEFGKEDLPVLYNMNFGHTEPKICLPYGAMAEINCDKSS
                     FSILESAVL"
     gene            2910..3281
                     /locus_tag="PLD67_04185"
     CDS             2910..3281
                     /locus_tag="PLD67_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002119671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HOT21751.1"
                     /translation="MEIKVIKINNVRIAKIYSNEILIKDGQSALDLLATVSYETDCDR
                     IIIDKSAIAEDFFDLSTKLAGEVLQKCINFHFKVAIIGDFSVYKSNSLKDFIYECNKG
                     KDIFFLPDENQAVEKLSIVRR"
     gene            3399..>4325
                     /locus_tag="PLD67_04190"
     CDS             3399..>4325
                     /locus_tag="PLD67_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOT21752.1"
                     /translation="MKSYRAQAIETLKKIFKDKSYSNIVINNDMKNVDNRFLSLYRKS
                     VLGVVENLIFIDWIINQVSKTKTKKMETDVLLVLRLAVYQIFFLDKSHENIVVNESVQ
                     YIKDNGNIRASKFVNAVLRNILRSKDNLIKKMNELPRDDYLSVKYSYPKELIVKWEKQ
                     FGKDVDKVLIANNTEAPLEIRVNTLKIQRDELIKLFEEKGIKSYKCKYADKGLIIENP
                     FEIDKTDEYKNGLFSVQSESSMLAGQILNPKENSFIIDVCAAPGGKTLNAAEMMNNTG
                     KILSRDIYPAKLRLIENEIKRLGIKNVKVEKYD"
BASE COUNT         1657 a          525 c          874 g         1269 t
ORIGIN      
        1 gatattcgtg agcaatataa gaaatatgat attacatttt ggtttaaagg cacagggtca
       61 cttttcaaac atgacggaac aatcaagaaa ataaatccgt ttaaacaagg aagtcatgca
      121 aaaaaatttg atataaacat taagaactct ggggaccgag cttgaaatat aaattttgtg
      181 attaatataa ctgcaaaata atgcgaggaa gacatatgga taatttagaa cgaattgctc
      241 aatttgagaa attcagcccc atgttgtatt tagactataa attaacgtca tggggacgaa
      301 tgctttgtga catagggaat aaagaaagct atacatctaa ttatgctgta atttacagaa
      361 atgaaaatgt tgacaatata attaaagaag tagaagaata ctataattca agaagcataa
      421 ttccaaaaat atttaatcgc tgcggctcgg tggaactgga tatattaaga ccatttttca
      481 caaagcatga ctatgcaata agagaatttg acatggattt gatgatttta gatacacata
      541 gtaccagaaa taaaattgca gaaaaagttg aggtaaaaat aataaaccgt gctcttgaag
      601 gtcaggaata taatttagca atagaacaag ataatggtga aacatatggt gtaaagcttt
      661 taaacaaaca aattgcttca ggaaacaata tgttttttgc ttataatgaa ttaaagaagc
      721 ctgtaagcat ggcattagct gaaaaatata atgatgttgt ctacataaca gatgtttata
      781 caacaccgtc acagaggtgc aatggatatg gaacggctgt cgtatatgct gttttatcac
      841 attatcataa ttcattgatt tacttgcata cagacaaccc ggatgcagca agtatttata
      901 gaagattggg attttcaggc aagagactaa agtcatggtg ggctgtaaaa ggttcattgc
      961 cggagtggtg tttggaataa tatctacaga aagtagatat gagtttgtag gagattcaat
     1021 ggaaaaactt caagatgatt acaatttaat aacaggcgtc tatataggtg atgtattgga
     1081 tgtccatttg taatgattga aaattaagga aacttgaaaa ttaggagaaa agtatgttag
     1141 acagtaaagg ttttgactta tgggcacatg gttatgataa gagtgtaaac cttagtgaag
     1201 aaaacaatga atatccattt gccggctaca aagatgtatt aaattatatt tataaccaag
     1261 ttaggaaaca taattattca agcgttttgg atatcggatt tggaaccgga atattaacaa
     1321 cacaactata taatgaaggt tatcatatta caggaattga tttttcacgc accatgattg
     1381 atatagctaa aagcgaaatg ccggaggcca tattaataaa ttgggatttt acgagagggc
     1441 tacctgtaga aattaaaaac aataaatacg attatataat aagcacatat gcaattcatc
     1501 atttaccgga tgaagaaaaa attaaattta ttaaatcact tttaaaactg ttaaatgtta
     1561 atggtaaaat attaattggt gatgtttctt tcatgacaag gaaagaatta gaagaatgta
     1621 aggaaacgca tacagaaatt tgggatgatg aagaatttta ttttgttgca aatgaaatga
     1681 tgatgagtct aaatgaattt tattgtaatt atgaaaagat atcgtattgc tcaggtatat
     1741 taatagttgc ggattatctg ttataattag taagaggaaa atttgaatta ttaagttaaa
     1801 tacttaaaaa aattctgtag gagaaattgt atatgaaaat tttaaaaaag cctaacaggt
     1861 taaaccgtgg agataaaata gcattagtaa gcttgtcctg gggcggtgca ggtgatgaaa
     1921 atttattgtg gcggtataaa caaggaaaag agcggcttga gcaagttttc ggcttggaag
     1981 ttgttgaaat gaagcataca ttggcgggaa cagaatacat atacaatcat cctgagaagc
     2041 gagcagaaga tttaatgaat gcgttccagg ataaatccat taagggtatt attgcatgta
     2101 tcggcggaat tgatagtata cgaatgcttc catttattga ttttgatata atcagtaata
     2161 atccaaagat ttttatggga tattccgata caacaacaac gcatcttttc tgttacaaag
     2221 cggggatatc gagtatttat ggaccgacac tattggttga ttttgcagaa aatgtttcaa
     2281 tgagtccata tacagttgaa catatagaaa aaactttgtt taaatcagag gcaataggag
     2341 aaatcgagcc ttcaccaaag tggaccagtg aatatcttcc ctgggaggga aaaaacaaat
     2401 taattcaaag gaaatttaaa gataacagcg gatatgaact cttgcaaggg aagggaatta
     2461 caaaggggcg attaatcgga ggctgtattg aagtattcga ttcgcttcgt ggtactgaag
     2521 tctttccaaa gttagaggat tttgatgata gtatactgtt tttagagacc tctgaagaaa
     2581 aaccgcctgc gtggattatt gaatgcagtt taagaattta tggaataaat ggtatattgg
     2641 atagaataaa aggaatgata tggggtaaac cgcaggatga aacatattat gcggaataca
     2701 aagaagctat aaaaaaagtt atgaaggaat tcgggaaaga ggatttacct gttttatata
     2761 atatgaactt cggtcatacc gagcctaaaa tatgtctgcc gtatggagct atggcggaaa
     2821 ttaattgtga taaatcatca ttttccattt tagaatcagc agtgctataa ggcaaaaaaa
     2881 agaaaaagaa ggattttatg gagcaaccta tggagataaa agtaataaaa ataaataatg
     2941 taagaattgc taaaatttac agtaatgaaa ttttaataaa ggatggtcag tcagccttgg
     3001 atttattggc aacagtttca tatgaaacag attgcgatcg tataataatc gataaatctg
     3061 caatagcaga agattttttt gacttaagca ccaaattagc cggagaagtg ttgcaaaaat
     3121 gtattaactt tcattttaag gtcgcaatca taggtgactt ttccgtatat aaaagcaata
     3181 gtttaaaaga ttttatatat gagtgcaata agggaaaaga tatattcttt ttgcccgatg
     3241 aaaatcaggc tgtagaaaag ttaagcattg tacgaagata gagtagtacc tcgtcccctt
     3301 aaggcgtgta ttcttgatat aatataaaaa gatgattgaa agaataaatt ggcaagatga
     3361 cagtaccccc gtccccttgt catctaaaaa gaggaattat gaaaagttac agagcgcagg
     3421 caattgaaac actgaagaaa atatttaaag ataaatccta ttcaaacatt gtgataaata
     3481 atgatatgaa aaatgttgat aataggttcc tttccttgta ccgaaaatcg gttttgggag
     3541 ttgttgaaaa tctaatattt attgattgga ttattaatca ggtatccaaa acaaaaacaa
     3601 agaaaatgga aaccgatgtc ttgcttgtgc tgaggttagc agtatatcaa atattctttt
     3661 tggataagtc tcatgaaaat attgttgtga acgaaagcgt tcagtatatt aaggacaacg
     3721 gtaatataag agcatcgaaa tttgttaatg cggtacttag gaatatattg cgcagcaagg
     3781 ataatcttat caaaaaaatg aatgagcttc cccgggatga ttatttaagt gttaaatatt
     3841 catatccaaa ggagttgata gttaaatggg aaaaacagtt tggcaaggat gttgataagg
     3901 tactgatagc caacaatacc gaagcacctt tggaaataag agttaatacc ttgaagattc
     3961 aaagagatga gttgataaag ctttttgaag aaaagggtat aaaatcttac aaatgcaaat
     4021 atgccgataa gggattgatt attgaaaacc cttttgaaat agataagaca gatgaatata
     4081 aaaatggact tttttctgtt caaagcgaaa gttcaatgct ggccggacaa attttaaacc
     4141 ctaaagaaaa cagctttata atagatgttt gcgctgctcc cggcggcaag acattaaatg
     4201 ctgcagaaat gatgaataat acgggtaaaa tattatcaag ggatatatac ccggcaaagc
     4261 taagactaat agaaaatgaa ataaaaagat tgggtataaa aaatgttaaa gttgaaaaat
     4321 atgat
//
LOCUS       DAOSOW010000118         4309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_20479_length_4309_cov_4.203338, whole genome shotgun sequence.
ACCESSION   DAOSOW010000118 DAOSOW010000000
VERSION     DAOSOW010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20479_length_4309_cov_4.203338"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="PLD67_04195"
     CDS             complement(<1..1317)
                     /locus_tag="PLD67_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21753.1"
                     /translation="MTVAIKKKINIPDPTMGPIGFTAASFKAAISGIGTILKKPKILF
                     TTLFISALQAALSYLKILMPSSLLVTTASFFTFAQGGMYAGLIGAAGGIIGKGIYAWF
                     MNTLFFSGSRDKSKDDKNENKKTKGKFALFLAGIGIALMVYNFLTGNASIENSVIGLT
                     SLAACLRALKRKNGFLIGFICSFTKGRMSRKAAAVVIKGMTLGFLAGVLSSLKFTGAI
                     CYLVGAVIFIIALILLAGRKRTLAAAAALLIILSNIVPAYSSIANFSDEWQDPYEAYG
                     IQDRTLQGYIALTDAMNASPPMSMDLTLIAVGVRNFDESGRIENRQVLALDGESETFG
                     IDSPEFSEFKFTSAVNLFLEDDGWYADSITQYSVNNAWTNYEREEGLYGNYQKCYFSF
                     KHLGAAGYLFEEYLEDGKVYITIPISFAYGNVMDQSQADSASEIFAR"
     gene            complement(1319..3193)
                     /locus_tag="PLD67_04200"
     CDS             complement(1319..3193)
                     /locus_tag="PLD67_04200"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HOT21754.1"
                     /translation="MKRNLMLCIFACILMLTIVTETAYGAEAFSITSYDIDITVYENN
                     SYAVIETINVRFIEQRHGIIRSLPLRTNRGKPALISHIDVFEHKFSTEKVSNNIEIKI
                     GDPDKYASFSERYTIGYVYTIGDDYLNDRDELYWNLIGNDWDCTIENVTFKINMPKSF
                     APDRLYFTHGQKGSTKHDGVDWYIDGNTIKGSLMISLKPNDSLTIAMPLPEGYFSQAE
                     RIKINDISYGWVLSLVCAALGTVLWVFMGRKKQIFPTVEFYPPQGLTSADVGYLIDGR
                     VDLFDITSLIIYWADKGYLSITEESVKNMFGTKKIFTLTKLRDIPEESQEYEKEMFEG
                     MFYMGDGINVRTDQLENRFYSVGERIKKQVKSSFEYNEETRIFKKTNTLCRILLLLSG
                     VICTLPFSYRVMKESGNVGEAGLMLKSIIMSAVIMAAFYFFMHILKNWKGMKKGERFK
                     QLFFALFISGAVCGTFIYSLSLSGNAIRGLISLAAAAILGILSNLCSRRTESGRWYEE
                     KLTGFKEFIKAAELDRIQLLADENPNYFYDILPFAMVLGVTDKWAKNFDSIMKEPPNW
                     YNSQGMQGRFSAVSFATGLDNDLKIFSLDIASKHQRSNSSISGGSSGGGSGGGGGRSW
                     "
     gene            complement(3205..3759)
                     /locus_tag="PLD67_04205"
     CDS             complement(3205..3759)
                     /locus_tag="PLD67_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOT21755.1"
                     /translation="MEILIGLVVIIVLLAISAIVSYNGFVKLRNQIEEAYSTMDVYLK
                     KRYEMIPNLVEVVKQYSIHEKETLERIVQARNMAINARSVEERGQNENILSGAIKSLF
                     ALSEGYPELKANENYLDLQNNLRTLEDEISQSRKYYNGVVKVMNNKVEIFPSNLFAKI
                     FGFNRYPYFMAEEYERQNVEIRFN"
     gene            complement(3788..>4309)
                     /locus_tag="PLD67_04210"
     CDS             complement(3788..>4309)
                     /locus_tag="PLD67_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21756.1"
                     /translation="LPDDSVFCSECGSKIVSVGNESGIDIDSINTNNESGYREQRGPV
                     PPVMRQMATHQPSEPYRKESIPRQPPEPYRRKSTPQQPSEPYRKEAYPQEPSESYIGA
                     SADIIGVGRYILMIFFAWIPIVNFILLIRWSFNKQKPNKSNFAKAMLVYNILALIVYV
                     LYIRLWLSVTSGI"
BASE COUNT         1211 a          939 c          747 g         1412 t
ORIGIN      
        1 ccgtgcaaat atttcggatg cgctgtctgc ctgggattgg tccatgacat taccgtatgc
       61 gaatgaaatg ggtattgtaa tataaacctt gccatcttcc aaatactctt cgaataaata
      121 gccggctgca cccaaatgtt taaatgaaaa ataacatttc tggtaattgc cgtacaagcc
      181 ttcttctctt tcataatttg tccaggcgtt atttaccgaa tattgtgtta tggaatcagc
      241 gtaccatccg tcatcttcaa ggaagagatt tactgcgctt gtgaatttaa attcggaaaa
      301 ctcgggacta tcaatgccga aggtttcact ttcaccatcc agggctaata cctgccggtt
      361 ttctattctt cccgactcat caaagtttct tactcccacg gctattaaag ttaaatccat
      421 ggacattgga ggggaagcat tcattgcatc ggttaatgct atataaccct gtaaggttct
      481 gtcttgaatt ccatatgcct cgtaagggtc ctgccattca tcggaaaaat ttgcaattga
      541 tgaataggcg ggcactatat tgcttaaaat tataagcagg gctgcagctg ccgcaagagt
      601 tcttttcctg ccggccaaga gaataagggc aattataaat attacagctc caaccagata
      661 acagattgct cccgtgaatt taagggatga caaaacacct gccaaaaacc ctaaggtcat
      721 tccctttata acgacagctg ccgccttcct tgacatccgc ccctttgtaa aggagcatat
      781 gaaacctatt aaaaaaccgt tctttctctt aagtgctctt aggcaggctg caagagacgt
      841 taagccgata acactgtttt ctattgatgc attgccggtt aaaaaattgt ataccataag
      901 tgcaataccg attcctgcca ggaatagagc aaacttaccc tttgtttttt tattttcatt
      961 tttatcatct ttgcttttat ccctggagcc tgaaaaaaac agtgtattca taaaccatgc
     1021 atatattcct ttgccgataa ttccgcctgc agctccaata agccctgcat acattcctcc
     1081 ttgggcaaaa gtaaaaaaac ttgctgttgt aaccaataaa gaagagggca tgagaatttt
     1141 caggtaggat aaggctgcct gtagcgccga tatgaataaa gttgtgaata gaattttcgg
     1201 tttttttagt atggtgccga ttccagaaat tgctgcctta aagcttgcag cggtaaaccc
     1261 gataggtccc atggtcgggt cgggaatatt aattttcttt tttattgcta cagtcatctt
     1321 accagctcct tcctccgccg cctcctgaac cgcctccaga gcttcccccg ctgatgcttg
     1381 agttgctcct ttggtgtttt gaggctatat ctaatgaaaa tattttcaaa tcattatcta
     1441 atcccgttgc aaaactaacc gcgctgaacc tgccctgcat gccttgcgaa ttataccaat
     1501 tgggcggctc tttcatgata gagtcaaagt ttttcgccca tttgtccgtt acgccaagta
     1561 ccattgcaaa gggtagtata tcataaaaat agttaggatt ttcatcagcc agaagctgaa
     1621 ttctgtctaa ttccgcagcc ttaataaact ccttaaatcc tgttaatttt tcttcgtacc
     1681 acctgcctga ttctgtcctg cggctgcaaa ggttcgacaa aattccaagt atcgctgctg
     1741 ctgctaatga aattaaaccc cttattgcat tgccggataa gcttaatgaa tatataaaag
     1801 taccgcacac tgctccgcta ataaacagag caaaaaataa ttgtttaaac ctttcccctt
     1861 ttttcattcc cttccaattc ttcaaaatat gcataaagaa ataaaaagct gccattataa
     1921 ctgcagacat tattatactc tttaacataa gtcctgcttc accaacattg cctgattctt
     1981 tcataaccct ataggaaaag ggcagtgtgc atattactcc tgacaagaga agcagaatgc
     2041 ggcacaaggt atttgttttt ttgaagattc gggtttcttc gttatattca aaacttgatt
     2101 taacttgctt ttttattctc tcccccacag agtaaaacct gttttccaat tgatctgttc
     2161 ttacatttat cccgtccccc atgtaaaaca ttccctcaaa catttccttt tcatattcct
     2221 ggctttcttc cggaatatct ctaagttttg taagtgtaaa gatctttttt gtgccaaaca
     2281 tattttttac gctttcttcg gtgattgaca aatatccttt atctgcccaa taaattatta
     2341 gagatgttat atcgaacaaa tctaccctgc cgtcgattaa gtaaccaaca tctgcgctgg
     2401 tcaacccttg aggggggtaa aattcaactg ttggaaatat ttgttttttg cgccccatga
     2461 atacccacaa gactgtgcct aaagccgcac agacaagaga taacacccat ccgtaagata
     2521 tatcattaat ttttattctt tctgcttgtg aaaaatatcc ttccggaaga ggcatggcaa
     2581 ttgtaagtga atcattaggc ttaagagaaa tcatcaagga gccctttatg gtatttccgt
     2641 caatatacca gtccacaccg tcatgctttg tgcttccctt ttgaccatgg gtgaaataaa
     2701 gcctgtcagg cgcaaaagac tttggcatgt ttattttgaa tgtaacgttt tcaattgtac
     2761 agtcccagtc atttcctatc aagttccagt aaagttcgtc tctgtcattt aaataatcat
     2821 ctcctatggt atacacataa ccaattgtat atctttccga gaaggaagcg tatttgtcag
     2881 ggtcgcctat tttaatttca atattatttg agactttctc agtagaaaac ttgtgctcaa
     2941 aaacatctat atgagaaatt agtgcaggct ttcctctgtt tgttctcaaa ggcagacttc
     3001 ttatgatgcc gtgcctttgc tcgataaacc tgacattaat tgtttcgatt accgcatagc
     3061 tgttattttc ataaaccgtt atatctatat cgtatgaagt aatactgaat gcttcagcac
     3121 cataagctgt ttccgtcaca atagttaaca tgaggatgca agcaaaaata caaagcataa
     3181 ggtttctttt cattgaccct acctctaatt aaatctaatt tctacatttt gacgctcata
     3241 ttcttcagcc ataaaatatg gatatctgtt gaatccaaaa attttagcaa acaagtttga
     3301 agggaatatt tcaaccttgt tattcataac tttaactacg ccgttatagt atttacggga
     3361 ctgagatatt tcatcttcca aggttctcag attgttttgc aagtccagat aattttcgtt
     3421 tgctttcaat tcaggatagc cttcagaaag agcaaagaga gattttatag caccggacag
     3481 gatgttttca ttctgccccc gctcttctac agaccttgca tttattgcca tgttccttgc
     3541 ctggacgatt ctttctaagg tttctttttc atgtattgaa tactgtttta ctacttccac
     3601 taaattcgga atcatttcgt atcgtttctt taaataaaca tccatggtgg aataagcttc
     3661 ttcaatttga tttcgaagct ttacaaaacc gttatatgac acaatcgctg atattgccaa
     3721 taaaactatg atgacaacca atcctattag tatttccatc ttcttcccct ttcttttata
     3781 gatattatta aatacctgaa gtcacgctca gccatagccg aatatacaaa acataaacta
     3841 ttaatgccaa aatattataa acgagcattg cctttgcaaa attgctttta ttgggttttt
     3901 gtttattaaa actccatctt ataagcagta taaaattgac tatgggaatc caggcaaaaa
     3961 aaatcataag tatataccgg ccgactccta taatatccgc acttgctcct atgtaggatt
     4021 cagacggctc ttgtgggtat gcttcttttc tgtatggttc agatggctgt tgtggtgttg
     4081 attttctcct gtagggctct ggcggctgac gcggtatcga ttctttcctg tagggctctg
     4141 acggctggtg cgtcgccatt tgtctcataa caggaggcac cggtcctcgc tgctcccggt
     4201 agccggactc attatttgta ttgatactgt ctatatctat tccggattca tttccaactg
     4261 atactatttt gctgccgcat tccgaacaaa acacagagtc atcgggaag
//
LOCUS       DAOSOW010000119         4302 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_20518_length_4302_cov_4.044737, whole genome shotgun sequence.
ACCESSION   DAOSOW010000119 DAOSOW010000000
VERSION     DAOSOW010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20518_length_4302_cov_4.044737"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PLD67_04215"
     CDS             complement(<1..711)
                     /locus_tag="PLD67_04215"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002867082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HOT21757.1"
                     /translation="MYKVDLNSDVGESFGNYKLGLDSEVLKYVSSANIACGWHAGDAM
                     VMQKTVDFAIKEDTCIGAHPGFPDLMGFGRREMKVTKEEAKNYVKYQIGALWAFAKSR
                     GTQLQHVKAHGGLYNMAAKDPELAMAIAEAIYEVDKDLILMGLANSEMIKAGKAVGLK
                     TANEVFADRAYNTDGTLVSRKLEGSMITDETLALSRVVRMVKEGKVQAITGEDIDIKA
                     DSICVHGDNPKAVEFVKTI"
     gene            complement(886..2295)
                     /locus_tag="PLD67_04220"
     CDS             complement(886..2295)
                     /locus_tag="PLD67_04220"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOT21758.1"
                     /translation="MKIRILIVDDEETIRLSLEEALKDLGYSISTAATGHIALEKVKE
                     YKPQIVFLDMRLAGENGLDILPKIKKIDKKVEVIIMTAYGDINTAVKAIKLGAFDYIN
                     KPFDLIDVEILIKRITENLKLQNKIYLLEKEKEKELNKKIIIGNHPLMNEVMNRIDIL
                     SKNDNVTVLIRGETGTGKDLVASYIHNHSIRRGSAMLKLNCGAIPQQLIESELFGFEK
                     NAFTGANNRKKGLLEIADGSTVFLDEIAEMPMEVQPKLLRFLDERKFKRVGGLEDIEV
                     DIRIIAATNKNLEKAIEDKEFREDLYYRLNVVPVYLPLLRERGDDILVLANNFLYEYN
                     CKFNKKIKGFTDRAKEALLLYSWKGNVRELKNVIERIVILVENEYIDIGDLPLEIRSA
                     LGGGNEFTKPSQDEMSSKLLSGGFSLENEVAEMEAEYIKLALKYCSNNHTKAAEMLGI
                     SRFAFKRRIEKYFYADNNA"
     gene            complement(2285..4042)
                     /locus_tag="PLD67_04225"
     CDS             complement(2285..4042)
                     /locus_tag="PLD67_04225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT21759.1"
                     /translation="MRNSIEKKILIPFIIILMVSILIIVIVFVVNGYNLLLDNHINIS
                     YRNLEEMILTIEKVNDDIKDEIGAKDFIINFYKDLGKNNLIIFSDDDDEILLNTVLNN
                     NFIEEFMNEELNKGPESISVGNYIFTYQKYDKWNWVIAYGLNRDNLLIEVMESQQYII
                     LVALIALVFSMHASIFITHNISKPIKMLAEHCNKIVADGIYKDRIEIDRNDEIGTLAD
                     AFNNMLDTVQNNTDRLIEATKFNEDILRNVSAGIVTADQHGSVISINQTAENLLIPAA
                     EKIGLVKECLHEQIMETLNTKMKINKILAFDDEHGNKKYIDVTTSLLIGGDGSISGAI
                     CNFNDITERKRIENSMSTLDRLTTIGQFASGIAHEVRNPLAGMKTSIQVLKNRLCKED
                     NDMIASNKKLLNGVLFEIDRLNNLITDLLNFAKPSFPNIEKTDLMGVLDKSLNLIGET
                     AKKNNIEINIDNNCKNTVAYVDKAQTEQIFINIINNAFFAMDKGGTLNISFNNCSQKE
                     KFIEVIFHDNGCGIQSQDLAKVFNPFFTTNPQGTGLGLSVVYELVMGNRGEIDIQSAV
                     NEGTKVIIRFPINGGSNEN"
BASE COUNT         1294 a          848 c          536 g         1624 t
ORIGIN      
        1 tattgttttt acaaattcga cagctttagg attatcacca tgtacacaaa ttgagtctgc
       61 tttaatgtca atatcttctc ccgtgatagc ctgaactttg ccttctttaa ccattctaac
      121 aactcttgat agtgcaagag tttcatcagt tatcatagag ccttccagtt tccttgaaac
      181 cagagtccca tcggtattat aagccctgtc cgcaaatact tcatttgctg tcttaagacc
      241 tactgccttg ccggccttta tcatttcgct gtttgcaaga cccatcaaaa ttaagtcttt
      301 gtcaacttca taaattgcct ctgcaattgc catagccaat tcaggatctt ttgctgccat
      361 gttgtataaa ccgccatgag ctttaacatg ctgcagctgt gttccacgac ttttcgcaaa
      421 tgcccacaaa gcacctattt gatatttcac atagttcttt gcttcctctt ttgttacctt
      481 catttctctt ctgccaaatc ccattaaatc agggaatccg ggatgtgcgc caatgcaagt
      541 gtcttcttta atagcaaaat caactgtttt ttgcattacc atcgcatctc ctgcatgcca
      601 tccgcatgca atatttgctg acgaaacgta ttttaggact tcagaatcca agcctagttt
      661 ataatttcca aaactttcgc caacatcact gtttaaatct actttgtaca aaattaatca
      721 cctccaaata aatattatgc aattaatatg ccaagattat ctttaataat aatctccata
      781 tttcaacaaa aaataaagag taaataaata tttgcgtgcg aatatgcacg ttttgccgag
      841 cgatagcgcc ctagttattg atgttctata tataattcta ccaaattatg cattattatc
      901 tgcataaaag tatttttcta ttctcctttt aaaagcaaat ctgcttatac ctagcatttc
      961 cgcagcttta gtatggttgt tgctgcaata tttaagagct agtttaatat attcagcttc
     1021 catttctgca acttcatttt ccaaagaaaa acctcctgaa agcagtttac tgctcatctc
     1081 atcttgactc ggctttgtaa attcattgcc tccgcctaag gcacttctaa tttctaaagg
     1141 caaatcgccg atatcgatat attcgttctc taccaatatt acaattcttt caataacatt
     1201 tttaagctcc ctgacattcc ctttccatga gtataataat agtgcttctt tggctctgtc
     1261 agtgaatcct tttatctttt tattaaattt acagttatat tcatataaaa aattatttgc
     1321 taatactaat atatcatctc ctctttcacg taaaagaggc agataaacag gcactacatt
     1381 caatctgtag tataagtcct ctctgaattc tttatcttct atggcttttt ccaagttttt
     1441 atttgtagcg gcaataattc tgatatctac ttcaatatcc tctagtccac cgactctttt
     1501 aaattttctt tcatctaaaa acctcaatag tttaggctgt acttccatgg gcatttctgc
     1561 aatttcatcc aaaaataccg tacttccatc agctatttca agcaatccct tttttctgtt
     1621 gtttgcaccg gtgaacgcat ttttttcaaa tccgaaaagt tcactttcta taagctgctg
     1681 aggaatagca ccgcaattaa gtttcagcat ggcagatcct ctccttatgc tgtgattatg
     1741 aatataagaa gctacaagat ctttgcctgt accggtttca ccccttataa ggacagttac
     1801 attgtcgttt tttgataata tatcaattct attcataact tcattcataa gaggatggtt
     1861 ccctattata attttcttat ttaattcttt ctccttttct ttctccaata aataaatttt
     1921 gttttgcaat tttaaatttt ctgtaattct ttttattaaa atttctacat caatgaggtc
     1981 aaagggtttg ttaatatagt caaaagcgcc gagttttatt gctttaacag cagtgttgat
     2041 atcaccatag gcagtcataa taataacctc gacttttttg tctatttttt ttattttagg
     2101 caaaatatcc aatccgttct cacctgctaa acgcatatct aaaaaaacta tctgaggttt
     2161 atattctttt accttttcaa gagcaatatg accggttgca gcagtactta tgctatatcc
     2221 caaatctttc agtgcttctt ccaaagataa tcgtatagtc tcttcgtcgt caactatcaa
     2281 tattctaatt ttcattacta cctccattaa tgggaaacct gattattact ttagttcctt
     2341 catttactgc actctgaata tctatttctc cccgattgcc cattaccaat tcatatacta
     2401 cagataaccc aagtcctgtt ccttgagggt ttgtagtaaa aaaaggatta aatacctttg
     2461 ctaaatcttg tgattgtatc ccacatccat tatcatgaaa aataacttca ataaattttt
     2521 ctttttgtga gcaattatta aaagaaatat ttaaagtacc tcctttatcc atagcaaaaa
     2581 aagcattatt gataatattg atgaatatct gctcagtttg agccttgtcc acatatgcaa
     2641 cagtattttt gcagttatta tcaatattta tttctatatt attttttttg gcagtctccc
     2701 cgattagatt taacgactta tctaaaactc ccattaaatc tgttttttca atattaggaa
     2761 aacttggctt agcaaaattc aataaatcag taatcaaatt attaagtctg tcaatttcaa
     2821 aaagtactcc gttaagtaat tttttattag aagctatcat atcgttatcc tctttgcata
     2881 acctgttttt taaaacttgg atacttgttt tcatgcctgc caagggattt cgtacttcat
     2941 gagcaatacc tgatgcgaat tggcctatgg tagttaatct atccaatgtg ctcatgctgt
     3001 tttcgattct ttttctttcg gtaatatcgt taaaattgca tatagctccg ctgatagacc
     3061 catctcctcc tatcaaaaga gaagttgtaa catctatata ttttttattt ccatgctcat
     3121 catcaaaagc aaggatttta tttattttca tctttgtatt taatgtttcc attatttgtt
     3181 catgcaaaca ttccttgaca agaccgattt tctcggctgc gggaatcagt aaattttctg
     3241 cggtctgatt tattgatatt acactgccat gctggtcagc agttactatt cctgctgata
     3301 cgtttcttaa aatatcttcg ttgaatttgg ttgcttcaat taatctgtct gtattgtttt
     3361 gcacagtatc gagcatattg ttaaaagcat cagccaatgt tcctatttca tcatttctgt
     3421 ctatttctat cctatcttta taaattccgt cagcaacgat tttattacaa tgttcagcca
     3481 gcattttaat cggttttgat atattgtgcg taatgaatat tgatgcatgc atagaaaata
     3541 caagggcaat taatgcaact aagattatat attgttgact ttccattact tcaattaata
     3601 agttatctct atttaagccg tatgctatta cccaattcca cttatcatac ttttgatatg
     3661 taaaaatata attgcctaca gatattgact ccggtccttt atttaattct tcattcataa
     3721 attcttcaat gaagttatta ttcaggacag tatttaacaa aatttcatca tcatcatcgc
     3781 tgaaaataat caaattgttt ttgcccaggt ctttataaaa attaataata aaatcttttg
     3841 ctccaatttc atcctttatg tcatcattaa ctttttcaat agtaagaatc atctcttcta
     3901 aattacggta tgaaatattt atatgattat ccaacaataa attatatcca ttaacaacaa
     3961 atacaataac aattattaaa attgatacca tcaatataat aataaaagga atgagtatct
     4021 ttttttcaat actgtttctc ataatttttg ccttcccttt ttacgagcgt attcacatga
     4081 ttataactga aatcaaaatg ctttctctaa atcatctcta ctatgattac acaattacca
     4141 taataaaaga attcaacaat aaataataga atcccgccta tatatcggaa atgtagcatt
     4201 acactacact tccaataaga ggattctatt atattgtacc ataaagtaag actaaagaaa
     4261 atatttttat agaataacaa taccttgtta ttttgttatt tt
//
LOCUS       DAOSOW010000120         4301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_20525_length_4301_cov_3.437824, whole genome shotgun sequence.
ACCESSION   DAOSOW010000120 DAOSOW010000000
VERSION     DAOSOW010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20525_length_4301_cov_3.437824"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLD67_04230"
     CDS             complement(<1..259)
                     /locus_tag="PLD67_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOT21760.1"
                     /translation="MRIISGTNKGKKLYAPEGKSVRPTGDKIKEAIFNIIGSIDEEST
                     VLELFAGSGSIGIEFLARGAKHCTFVDISRKSLNYVKRNLEL"
     gene            complement(284..2338)
                     /gene="recG"
                     /locus_tag="PLD67_04235"
     CDS             complement(284..2338)
                     /gene="recG"
                     /locus_tag="PLD67_04235"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOT21761.1"
                     /translation="MLDADIQYLKGVGPNRALKLHKLDIFTVSDLLNYFPVKYEDRRT
                     VKNINEICEQGKYLLKVRIFEKPKYSRIKKNMSIIKAICKDDTGFITLTFFNQDFLKD
                     KLIVGEYYYVFGNVKIAYGKLESTNPEIEFINKDWKAGKIMPVYGLTYGLSNNEITKL
                     VKTALETYYDEIQNVLPDEIIKKYGLISKKAAVKALHFPSDNKKYTLAKRTIAFEKLL
                     IMQLGLFTIKNNLTNELNEIVLRNTELSEKLLSSIPYKLTKAQLKVIEEIKEDLKSQK
                     TMNRLVQGDVGSGKTVVAVYAMLMAVGSGYQSSMMAPTEILALQHYDTIKEYIKIAGL
                     DVKAEILTGSTKPKEKQEILDKLKGGEIDIIVGTHALIEENVEFKNIGLVVTDEQHRF
                     GVRQRARLSNKGKNPHILIMTATPIPRTLALMIYGDLDISLIDEMPPGRQKVITSIIS
                     EKEKTKAYDFIKSQVDSGRQAYIVAPLVDESENLDLNSAVEIYKELSTSHFSGYKLGL
                     LHGKMSNSEKEEIINKFYRGKINVLISTTVVEVGVNVPNAVVMLILNAERFGLAQLHQ
                     LRGRVGRGKCQSYCILVNESKSKKSKDRMNILKRTDNGFIIAEEDLKIRGPGDFFGVR
                     QHGLSKLEIQNIVNNVETVQEVQLLSKRILSENPYLKGSDYQNLKMEIMNLFKDETIV
                     FN"
     gene            complement(2382..4001)
                     /locus_tag="PLD67_04240"
     CDS             complement(2382..4001)
                     /locus_tag="PLD67_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOT21762.1"
                     /translation="MLIKYIDNVKFKKMLLGAAVNLENNKTIVDSLNVFPVPDGDTGT
                     NMNLTLQAAIKEINNLSESTITSLASAAANGSLMGARGNSGVILSQLLRGMSKALKGL
                     NHIDCKIIAESLKSASDTAYKAVMKPVEGTMLTVARETAEKAMATYTDYEDVIEFIES
                     LIQQAKDTLNRTPEMLNVLKQADVVDAGGKGLIYLMEGALAALKGVEIKQEITEQAVI
                     DTEVELDEFTSEDEIIFAYCTEFLIKNPIKTAEEFLTRIYDKGDSIVCAGNENVIKIH
                     IHTNNPGQILAMAVKFGELSNIKIDNMKEQFRERLKENHKNNQKKKKYGFVSIGVGKG
                     IEKVFTDLNVDVFLSGGQTMNPSTEDILEAANDINAEHIIILPNNSNIILAATQAKKI
                     SDKNLYVVPSKTIPQGIAALIAFKEDSDIDENIEAMSQAVKEVKTGLVTYAVRDTVFN
                     DLEIKKDQIIAIYEGDITNHGENPEDEAVELINKMVDEDSFLVTVFYGDNVDEKTAES
                     IKPRIEEIAEDCDIEIIYGGQPLYYYIISVE"
     gene            complement(4015..>4301)
                     /locus_tag="PLD67_04245"
     CDS             complement(4015..>4301)
                     /locus_tag="PLD67_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HOT21763.1"
                     /translation="AAMECYGVVGMASRNATQGFFELLKREQLTKGIKVTSTEEDKIN
                     IDLYIVLQYGVKISVVAENVISRIKYSVETFSGVSVENVNIFVQGVRVQK"
BASE COUNT         1163 a          881 c          594 g         1663 t
ORIGIN      
        1 ataattccaa attccttttc acataattta aacttttacg agatatatca acaaatgtac
       61 agtgttttgc acctcttgcc aaaaattcga tgcctatgct tcctgagcct gcaaacaatt
      121 ctaatacagt cgactcttca tcgattgacc cgattatatt aaaaatcgcc tctttgattt
      181 tatcaccggt aggtcttaca cttttccctt caggagcata caattttttg cctttatttg
      241 ttccggatat tattctcatt agaacctctt tttattttat ttatcaatta aatacaattg
      301 tttcgtcttt aaacaaattc ataatttcca tttttaaatt ttgataatca gaacctttta
      361 aatatgggtt ttccgataaa attctttttg ataaaagttg gacttcctgt actgtttcta
      421 cattattcac tatattttga atttccaatt ttgataatcc atgctgtctt actccgaaaa
      481 agtctccggg tcctcttatt tttaaatctt cttctgctat tataaacccg ttgtctgttc
      541 tttttaaaat attcattcta tcttttgatt ttttactctt actttcatta accaaaatac
      601 aatatgattg gcatttacct cttccgactc tgcctcttag ctgatgcagc tgtgcaagac
      661 caaacctttc agcatttaaa ataagcataa caactgcatt tggaacattt acgccaactt
      721 cgacaacggt ggtagagata agcacattta tttttcccct ataaaattta tttatgattt
      781 cttctttttc actattgctc attttaccgt gcaaaagacc taatttgtac cctgaaaaat
      841 gtgaggtgct taattcttta tatatttcaa cagcggaatt caaatctaaa ttttcggatt
      901 catcaaccaa gggtgccaca atatatgcct gcctgccgct gtcaacttga cttttaataa
      961 aatcatatgc ttttgtcttt tccttttcgc ttatgataga agtaattact ttttgtcttc
     1021 ccggaggcat ttcgtctatc aatgaaatat ccaaatcacc ataaatcatc aaagccaagg
     1081 ttcttggaat aggtgtcgct gtcataataa gaatatgcgg gtttttcccc ttatttgaca
     1141 gccttgctct ttgccttacc ccaaagcgat gctgttcgtc ggtaaccaca agccctatat
     1201 ttttaaactc cacattttct tcaataagcg catgggttcc aactataata tcgatttctc
     1261 cgcctttgag tttatcaaga atttcttgtt tttcttttgg ctttgttgaa cctgttaaaa
     1321 tttctgcctt aacatccaat ccggctattt ttatatactc tttaattgta tcatagtgct
     1381 gcagggctaa tatttcggtt ggagccatca tggatgactg atatcccgag ccgactgcca
     1441 ttagcattgc atagacagct accacagtct ttccggagcc gacatccccc tggacaagtc
     1501 tgttcattgt tttttgactt ttcaaatctt cttttatttc ctcaataact ttaagctgtg
     1561 ccttagtaag cttgtaaggt atggagctca atagtttttc tgacagctct gtgtttctta
     1621 atacaatttc atttaattca ttggttaaat tattctttat agtaaaaaga ccaagctgca
     1681 ttatcaataa tttttcaaag gcaatagttc tctttgccaa tgtatatttc ttattgtcgc
     1741 ttggaaaatg aagagctttt actgcagctt tttttgaaat aagcccatat ttctttatta
     1801 tttcgtcagg caagacattt tgaatttcat catagtatgt ttctaaggca gtctttacta
     1861 actttgtaat ttcattattt gacaatccgt atgtaagacc gtaaacaggc attatttttc
     1921 ctgccttcca gtctttatta ataaattcga tttcaggatt tgtagattct aatttgccat
     1981 aggcaatttt tacatttcca aaaacatagt agtattcgcc aacaatcagc ttatccttta
     2041 aaaagtcctg attgaaaaat gtaagggtga taaaacccgt atcatcctta catatagctt
     2101 taattattga catattcttt ttgattcttg aatatttggg tttttcaaaa atcctgactt
     2161 ttaacagata tttaccctgc tcacatattt catttatatt tttaactgtt ctcctgtctt
     2221 catatttaac gggaaaataa tttaataaat cgctcactgt aaatatgtct aatttgtgca
     2281 attttaaagc cctgttcggc cctactccct ttaaatattg aatatctgca tctaacattt
     2341 ttcaccacta catgttatag aagtctgcct gccttgtttt gttattctac cgagattata
     2401 tagtaataaa gaggctgacc tccgtatata atttcaatat cgcagtcttc tgcaatctct
     2461 tcaatccttg gtttaatact ttctgcagtt ttttcatcga cattatctcc gtaaaatacg
     2521 gtaactaaaa aactgtcttc gtcaaccatt ttatttatta attcaactgc ttcgtcttcc
     2581 ggattttctc cgtgatttgt tatatcgcct tcataaatag caataatttg gtctttttta
     2641 atttctaaat cattaaatac agtatccctt acagcataag ttacaagccc tgtttttact
     2701 tcctttaccg cctgtgacat ggcttcaata ttctcgtcta tatcactgtc ttctttaaat
     2761 gcaataagag ctgctatacc ttggggtata gtcttgcttg gcaccacata taaatttttg
     2821 tccgaaattt tctttgcttg tgttgcagct aatatgatat tggaattatt gggtaatatt
     2881 ataatgtgct ctgcatttat gtcgtttgct gcttccaata tatcttccgt gcttggattc
     2941 attgtttgtc cgccggacaa aaacacatct acatttaaat cagtaaatac tttttctatt
     3001 ccctttccta cgcctatgga aacaaaaccg tatttctttt ttttctgatt atttttgtga
     3061 ttttccttaa gtctttccct aaattgctcc ttcatgttgt caattttaat gtttgaaagc
     3121 tcaccgaatt taactgccat agccaatatt tggcctgggt tgttggtgtg tatgtgaatt
     3181 tttattacat tttcattgcc ggcgcaaact atggaatcac ccttgtcata aattcttgtt
     3241 aaaaattcct ctgccgtctt tatgggattt ttaatcaaaa attcagtgca atatgcaaat
     3301 ataatttcat cttcgctcgt aaattcatct aattcaactt ctgtatcaat cacggcctgc
     3361 tctgttatct cctgtttgat ttctactcct tttagtgccg caagtgcacc ttccataaga
     3421 tatattaatc cttttccgcc tgcatccaca acatctgcct gttttaaaac attaagcatt
     3481 tcaggagtgc gatttaaagt atcttttgct tgctgaataa gactttctat aaattctatt
     3541 acgtcttcat aatctgtata agttgccatt gctttttctg ccgtttccct tgcaactgta
     3601 agcattgttc cctcaacggg cttcattacc gctttataag cagtatctga ggctgattta
     3661 agggattctg ctattatttt gcaatctata tgattaagac ccttaagtgc ttttgacatt
     3721 cctcttaaca attgagacaa tattactccg gagtttcctc ttgctcccat aagtgagccg
     3781 ttagctgccg cacttgcaag agatgtaatt gtgctttctg aaagattgtt tatttccttt
     3841 atagctgcct gcaaggtaag attcatattc gtaccggtat ctccgtcggg aacaggaaat
     3901 acatttagag aatccacaat tgttttattg ttttcaaggt taacggcagc ccccaaaagc
     3961 atctttttga atttgacatt atctatgtac ttaattaaca ttttagcctc ctgattactt
     4021 ttgcaccctt acaccctgca caaaaatatt aacattttca acgcttactc ccgaaaaagt
     4081 ttcaacgctg tacttaattc tggaaattac attttctgct acaacggata tttttactcc
     4141 atattgcaat actatatata aatctatatt tattttatct tcttcggttg atgtaacttt
     4201 tatccctttg gttaactgct ctcttttcag cagctcaaaa aaaccttgag ttgcatttct
     4261 ggaagccatg cccacaacac cataacactc cattgcagct a
//
LOCUS       DAOSOW010000121         4291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_20582_length_4291_cov_3.660293, whole genome shotgun sequence.
ACCESSION   DAOSOW010000121 DAOSOW010000000
VERSION     DAOSOW010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20582_length_4291_cov_3.660293"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(95..1261)
                     /locus_tag="PLD67_04250"
     CDS             complement(95..1261)
                     /locus_tag="PLD67_04250"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOT21764.1"
                     /translation="MNNFTVPFSFTLPTKIVYGPGCISSLIDELHLNNGNRPIIVTDK
                     GVRNAGILDKITFLLERNNTDYIVYDGVEPNPKDVNVEEGARIAREFGADSIIAIGGG
                     SPIDCAKSVGVLLAHKEDKIKKYDGKTAATKPLPLLITIPTTSGTGSELTFSSVITDT
                     LNNYKMTVKSQYTAAKVAICDPELTLSLPAHITASTGMDALTHAIEAYTATCAEPISD
                     AMALYATELIYNNLVTAFNDGVNLEARSAMLLGSMLAGIAFSHSDVASVHCIAESLGG
                     VYDLPHGVCNAVFLPHVMEYNMEYSVKRYARIAKAMGLSFENEREGAKKAVEAIKKLA
                     VDVKLPLFSSLKVDINDFEKIAEMSVKNISTESNPRPMTKEDYLTVIENAYKHK"
     gene            1432..2541
                     /locus_tag="PLD67_04255"
     CDS             1432..2541
                     /locus_tag="PLD67_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT21765.1"
                     /translation="MTQMIYTVQPGDTLYSIARLYGSTIQAIVDANNILNPNLIYPGS
                     VILIPVEEEYLETPPGSLIYTVQPGDSLYIISLLFKVSIQRILELNDIPDPSLIYPGM
                     KIILPPEALNPFQPIVPGIIRYTVLPGDTIYKIAARFGTTAQSIINANPGLDPRRLIP
                     GTVITITIPENAVAIYRGNPNRRMVALTFDATYGDNQTYELLEILRNNNIKATFFLSG
                     IWLINYPDLARAIAAEGHEIGNHSLTHPHMPLITMQEVTNQIVRTEALIRNITGQDPY
                     LFRPPYGEYTQAILNQLASLGYVTILWTIDSLDWQNPGAAAIVSRVVNNAEPGAIILL
                     HQSAPDTLEGLQSMITQLRQQGYDFGTVTQVISPL"
     gene            complement(2612..3394)
                     /gene="thiD"
                     /locus_tag="PLD67_04260"
     CDS             complement(2612..3394)
                     /gene="thiD"
                     /locus_tag="PLD67_04260"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOT21766.1"
                     /translation="MKKVLTIAGSDSSGGAGIQADLKTMTSHKIYGMSVITALTAQNT
                     TGVYGVFESSPEFVAQQMDAVFTDIYPDAIKIGMVSNKEIISVIADKLIEYKAGKIVL
                     DPVMVATSGGKLFKDDAVEMLINKLIPLATVITPNIQEAQVLSGIEIESKEDMQRAAV
                     KISKNYSGYILIKGGHLTLSSDDLLYSNGEFIWYEQPKINNLNTHGTGCTLSSAIACN
                     LAEGNDVKTSILNAKKYITGAIKSNLNIGKGRGPLNHCWNLG"
     gene            complement(3405..4043)
                     /gene="thiE"
                     /locus_tag="PLD67_04265"
     CDS             complement(3405..4043)
                     /gene="thiE"
                     /locus_tag="PLD67_04265"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010250976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOT21767.1"
                     /translation="MKLDSKSLLLYAITDRAWLKGKTVAEAVEEAILGGATCIQLREK
                     NLSDEEFLKTAMDVKAVTDKYNIPFIINDNVDIAVRVGADGAHIGQDDEEIKSAREKL
                     GADKIIGLSAATVEEAVQAEQSGADYIGVGAVFNTLTKLDANTVSFETLKEICNTVKI
                     PVVAIGGISKDNALQLAGTGIEGISVVSAIFAQNDIKTAASELLELAKKIIE"
     gene            complement(4027..>4291)
                     /locus_tag="PLD67_04270"
     CDS             complement(4027..>4291)
                     /locus_tag="PLD67_04270"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019242140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOT21768.1"
                     /translation="HPMMGKISGTGCMLSSIIGAYCGANKDNIVDATAAAVCIIGLSG
                     ELAYEKILKNDEGTSMLRSHLIDFMSKIDDKILEEGAKFEIRQ"
BASE COUNT         1213 a          922 c          746 g         1410 t
ORIGIN      
        1 ggctgtaatg gcagtaagcc tcttattgtt catataacct aaacccttcg cttcgcgaag
       61 ggtttttcat gacaagggga cgggggtagt gtcatcattt atgtttgtag gcgttttcaa
      121 tgacagtcaa atagtcttct tttgtcatgg gtctcgggtt gctttcggtt gaaatatttt
      181 taactgacat ttctgcaatt ttctcaaaat catttatgtc tacctttaag cttgaaaaca
      241 agggcagttt aacatcaact gctaattttt ttatcgcttc aacagccttt tttgctccct
      301 ctctttcatt ttcaaagctt agacccatag ctttggctat tcgtgcatat ctttttacgg
      361 aatattccat gttatactcc ataacatgag gcagaaatac agcattgcag actccgtgag
      421 gaaggtcata tactcctccc aaagactcag ctatgcaatg gacagaagcc acatcggaat
      481 ggctgaatgc tatgcctgca agcatgctgc caagcagcat tgcgcttctt gcttcaagat
      541 tgactccgtc attaaaagca gtaacaagat tattgtaaat taattctgtt gcatacaaag
      601 ccatggcgtc acttatgggc tccgcacaag ttgcagtata agcttcaatt gcatgggtta
      661 gagcatccat gccagtggaa gcggtaatat gtgcagggag tgataaggta agctccgggt
      721 cgcaaattgc aaccttcgct gcagtatatt gacttttaac agtcatttta taattattca
      781 gtgtatccgt aataacggaa gaaaatgtca gctcgctgcc ggtgcctgaa gttgtcggga
      841 tggttattaa cagaggcaag ggctttgtag cagctgtttt gccatcgtat ttcttgattt
      901 tatcttcctt gtgagctaag agcactccaa cagatttagc gcaatctatc gggcttcctc
      961 ctccaatggc aataattgaa tctgcaccga attctcttgc tattcttgct ccttcttcta
     1021 cattcacatc ttttggattt ggctcaacac catcataaac aatgtaatct gtattgtttc
     1081 gctccagcaa aaaagttatc ttatccagta tgcctgcatt tctaacacct ttatcggtaa
     1141 caataatcgg tctatttccg ttgtttaagt gcaattcgtc gattaaagaa gagatgcatc
     1201 ccggtccata cacaatcttt gtcggcaaag taaaactaaa tggcacagta aaattattca
     1261 ttcaaatcct ccttggtaat taacgatatc cgtagttaca tatctttatt atattacatg
     1321 tccgaaatat ttcaacacta ttaaatgaca aaggacgggg tagtgtcata ttcatactat
     1381 cactttgtat aatattcgga atattatata gaaaagaata ggaggattat tatgactcaa
     1441 atgatatata cggtacaacc cggtgacacg ctttatagta ttgctcgcct gtatggaagt
     1501 acaatacaag caattgtaga tgcaaataat attttaaatc caaaccttat atatcccggc
     1561 tctgttatat tgatacctgt tgaagaggaa tacttagaaa ccccgcccgg aagccttatt
     1621 tataccgtac agcccggcga tagcctctat attatttctc ttttattcaa ggtaagcatt
     1681 caaagaattt tagaattaaa cgacattccc gacccttcat tgatttatcc gggaatgaaa
     1741 attatactgc ccccagaagc tcttaatcca tttcaaccta tagtgcccgg cataatcaga
     1801 tataccgtac tgcccgggga tactatatat aaaattgctg caaggtttgg aacaacagct
     1861 cagtctatta taaatgctaa tcccggtctg gaccctcgaa gacttattcc gggaacggta
     1921 attacaatta caattcctga aaatgctgtt gccatataca gaggaaatcc aaacagaaga
     1981 atggttgctt taacatttga tgctacttac ggtgataacc agacttatga attgcttgaa
     2041 atattaagaa acaataatat taaggcaaca tttttcctgt ccggaatctg gttgataaat
     2101 tatcctgatt tagcaagggc tatagcagct gaaggtcatg aaataggaaa ccacagcctt
     2161 acccaccctc atatgccgct tataacaatg caggaagtca caaaccagat tgttcgaaca
     2221 gaagctctta tcaggaatat cacgggtcaa gacccatatt tgttcagacc gccttatgga
     2281 gaatatacgc aagcaattct aaaccaattg gcttcacttg gctatgtaac aatcctgtgg
     2341 acgattgact ccttagattg gcaaaatccg ggagctgctg caatagtatc gagagttgta
     2401 aataatgcag agcccggagc aattatcctg ctgcatcaat cggcacccga cacacttgaa
     2461 gggctgcaat caatgataac acaattaaga cagcaaggat acgatttcgg aacagttaca
     2521 caagtaataa gtccgctata atcacaagag gacggggttg tcataatcat taccaaccac
     2581 gtccgcttac ttttgtaagt gctataagtt tttaacctaa attccagcag tgatttaaag
     2641 gtcctcttcc ttttcctata tttaaattag attttattgc gcctgttata tatttttttg
     2701 catttaatat gcttgtttta acatcattgc cctctgctaa attgcacgct atcgccgatg
     2761 aaagcgtgca gcctgttcca tgtgtattta gattatttat cttgggctgc tcataccata
     2821 taaattcacc atttgaatat aataaatcat cgctgctaag agttaagtgt cctcccttga
     2881 taagaatata gcccgaataa tttttggata tttttaccgc tgctctttgc atatcttctt
     2941 tgctctcaat ttcaatcccg ctaagcacct gagcttcctg tatgttgggt gttattacgg
     3001 ttgctaaagg aatcagctta tttataagca tttctactgc atcgtcctta aaaagtttgc
     3061 ctccgctcgt ggcaaccatt actgggtcaa gcactatttt tcctgctttg tattcaatca
     3121 gcttgtctgc aataacagat ataatttctt tattcgaaac cattccgatt tttattgcat
     3181 cgggataaat atccgtaaaa accgcatcca tctgctgagc aacaaattca ggcgatgatt
     3241 cgaatactcc gtatactcct gttgtatttt gagccgtcag tgctgttata acactcatac
     3301 cgtatatttt gtggctggtc attgttttta agtcagcctg tattcctgct cctccgcttg
     3361 agtcagaacc ggctattgtt aatacctttt tcatattact tcctctattc tattattttt
     3421 tttgctaact ctaaaagttc tgatgcagca gttttaatat cattttgtgc aaaaatagcc
     3481 gatacaacag atattccctc tatgcccgta cctgcaagtt gaagtgcatt atctttggat
     3541 attcctccga ttgcaaccac aggtattttc acggtattac atatttcctt taaggtttca
     3601 aatgataccg tatttgcatc aagttttgtt aaagtattaa aaactgcacc cactcctata
     3661 taatctgcac cgctctgctc ggcttgaaca gcttcttcca ctgtagcggc agaaagaccg
     3721 attattttat cagctcccaa tttttctctg gctgatttta tttcttcgtc gtcttggccg
     3781 atgtgtgctc cgtcagcacc gactctaact gctatatcta cattatcatt gattataaat
     3841 ggaatattgt atttgtcagt aactgcctta acatccatgg cagtttttaa aaattcttca
     3901 tcgcttaaat ttttttccct tagctgaata caagttgctc cccccagtat tgcctcttcc
     3961 acggcttcgg caactgtttt tccctttaac caggctctat cagtaattgc atataatagc
     4021 agtgatttac tgtctaattt caaatttcgc accttcctcc aatattttat catcaatttt
     4081 gctcataaaa tctattaaat gtgaccttag cattgatgtt ccttcatcat tctttaaaat
     4141 tttctcataa gctaattcac cgcttaatcc gatgatacat acagcagctg ctgtggcatc
     4201 gacaatattg tccttgttag caccgcagta tgcaccgatt atagacgaaa gcatacaacc
     4261 tgtaccggat attttaccca tcattggatg t
//
LOCUS       DAOSOW010000122         4284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_20622_length_4284_cov_3.430598, whole genome shotgun sequence.
ACCESSION   DAOSOW010000122 DAOSOW010000000
VERSION     DAOSOW010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20622_length_4284_cov_3.430598"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PLD67_04275"
     CDS             <1..692
                     /locus_tag="PLD67_04275"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT21769.1"
                     /translation="RTITRAEFSTMLNKAMKIEITENINFSDVREKEWFYKEIQKSVA
                     AGFFSGYENNTFRPNNPIKREEVAKVVAGAITTGNIDGEGATLLKDYSTIQEWAKDSV
                     NTVYNKGYILGYPDKTYMPSKALTRAEAVKIIFEIVDNENIEYGFNITNYNETYSSAV
                     VVGNLNVLDSVGNGNVYIKNVVVLGDIVISAKNVKSVELTDVKARRIIVEGENNPVRV
                     VYNDNVIIAKK"
     gene            complement(738..2483)
                     /locus_tag="PLD67_04280"
     CDS             complement(738..2483)
                     /locus_tag="PLD67_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21770.1"
                     /translation="MEKKNNFMPLLIYSIKEKKNWVLLSTAIIFLTTLLIPYILRVDE
                     EFFIMFGIVELFVLVLINCLVDNSFFHNESKLTYYSSKPVTLGKQIIINIVTNAVFTV
                     YLLALIVLSVIFQGLDYEVLKVFKMLIPWLSAIILLASLSSILSGNTLMAGAMTIFNF
                     CLPLIILLVIMFIFSILENLVLGFSADVLSDYFVNNIYRIDYLYFVRYVESKSIDLVY
                     LLLLPIILIFISLLINKFIKRRKNENTGFIVFNGFKYFVAVLACLIIPASFSIGFSRY
                     MSIGNRLIISALLAVLSYYIIIAFIEKSFRISKSSVKVFIVSMVAFAVITGGTVGVAS
                     QYENLVPEAEDVQMAYVGNQRWSINEIDRFLRGDYTSEKSDFPEWKRNRNIITYEDKD
                     IIENITELHEEILKDQTFYHESDYYGINNFVVAYWMKDGSKIIRDYTLTATDKLTKEH
                     ETKNELANKLLNSIDYKKQKFYYIYDENYYSGRNLYADFKNISNLNNVIKSVYLNDLR
                     GALTKDIENLFIENDKAFIELSMNYDSRFNKAVMAGEQEYILEIYEKISDIDKNYINE
                     INLNKNFVNTLEMLK"
     gene            complement(2467..3360)
                     /locus_tag="PLD67_04285"
     CDS             complement(2467..3360)
                     /locus_tag="PLD67_04285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21771.1"
                     /translation="MMLTINNLSKKFGDFQVLKNININAEKGRIYGLVGSNGCGKTTI
                     LKHIMKVYKQDEGHISFNGEEVVEGSDIIKEFYFVQDTLYFPYQYTLNKLFHYEKLMY
                     NMSEEKFEYLLKYFDIDKNKVLNKMSKGQKKQAAFVLSIAAQPRLLLLDEIVDGLDAV
                     IKRKFWDVLIKEIIDNNMTVVISSHDLKELDNICDRVGIMHEGRILREENLEKMKEDI
                     KRVQFAVEGEFDPDKLKEFNVLQVVKIGSVFIATLTGNIDELKKYLESKYTLLLFDKL
                     SMSLEEIFITELGGVGYGKEK"
     gene            complement(3344..3736)
                     /locus_tag="PLD67_04290"
     CDS             complement(3344..3736)
                     /locus_tag="PLD67_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT21772.1"
                     /translation="MIRIDNDNPKAIYEQIYDEIVRLIFSKSLKPDEKLPSVRELASM
                     IRINPNTIQKAYKYLEEDNYIYSIKGVGNFVKDADELRNLHLKNMKDELQKVVKSLKD
                     LGLRNERIIEMVRDILTPGTKGGHDVND"
     gene            complement(3751..>4284)
                     /locus_tag="PLD67_04295"
     CDS             complement(3751..>4284)
                     /locus_tag="PLD67_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21773.1"
                     /translation="IKIVLTHTNTVVCRTLKAFSKKPYNHISISFENDCSTMFSFGRK
                     ITWFPLVGGFIKEDINSGVFKMHPETKCKIYKFEVTDEDYDIIIKRLNDFLAKPHKYR
                     YSFLNVFLIRFNIPYQREYHFVCSSFVSYLLKGVIPFNKEISLIIPDDYNNFNLIPEY
                     EGRLFEYVSKKVACVNA"
BASE COUNT         1441 a          758 c          569 g         1516 t
ORIGIN      
        1 acagaactat tacaagagct gaattttcaa caatgttaaa caaggcaatg aaaattgaaa
       61 taaccgaaaa tataaacttt tccgatgtaa gagaaaaaga atggttctat aaggaaattc
      121 aaaaatctgt agctgccgga tttttttcag gatatgaaaa taatacattc agaccaaaca
      181 atcccatcaa acgggaagaa gtagcaaaag ttgttgcagg tgccataaca acgggaaaca
      241 ttgacggtga aggtgctacg cttttaaagg attacagtac tattcaggaa tgggcaaaag
      301 atagtgtaaa tactgtttac aacaaaggat acatactagg ctatcccgac aaaacgtaca
      361 tgccttctaa agcattaacc cgtgccgaag cagtgaaaat tatctttgaa atagttgata
      421 atgaaaatat tgaatacggg tttaatatta caaattacaa tgaaacttac agcagcgcag
      481 tagtggttgg aaatttaaat gttttggatt ctgtcggcaa cggtaacgta tatattaaaa
      541 atgttgtggt tttaggtgac attgtaattt cggctaaaaa tgttaaatcg gttgagctta
      601 cagatgttaa ggcaagaaga ataatagttg aaggtgaaaa taatcctgta agagtagtgt
      661 acaatgacaa tgtaataatt gcaaaaaaat agatataaaa agattcttca tcacgaagaa
      721 tcttttttgt tgccggttta tttcagcatt tcaagcgtat ttacaaaatt cttatttaaa
      781 ttaatttcat ttatataatt tttatcaata tcagaaattt tttcatatat ttctaatata
      841 tattcttgtt ctcccgccat aaccgcctta ttgaaacgag aatcgtaatt cattgacaac
      901 tcaataaagg ccttatcatt ttcaataaat aaattttcta tatcttttgt caatgcacct
      961 cttaaatcgt ttaaataaac actctttata acattattaa gattactaat atttttaaag
     1021 tctgcataca gatttcttcc gctgtaatag ttctcatcgt aaatataata gaatttttgc
     1081 tttttataat caattgaatt cagcaatttg tttgctaact catttttagt ttcatgctct
     1141 tttgtaagtt tatctgtagc agttaaagta tagtctctta ttattttact tccatccttc
     1201 atccagtaag ctactacaaa gttatttatt ccgtaataat cagattcatg ataaaatgtt
     1261 tggtctttta atatttcctc gtgaagttca gtaatatttt cgataatatc tttatcttca
     1321 taagtgatga tatttctatt tcttttccat tccggaaaat ctgatttttc acttgtatag
     1381 tcgcccctta aaaacctgtc aatttcattt attgaccatc tttgattgcc cacatatgcc
     1441 atttgaacat cttccgcctc aggaaccaga ttttcatatt gacttgcaac ccctacggta
     1501 cctcccgtta ttacagcaaa tgcaaccatg ctcactataa acaccttaac tgaagatttg
     1561 gatattctga atgatttttc aatgaatgca attattatat agtaggatag tactgccaat
     1621 aatgctgata taattaacct gtttccaata ctcatatatc ttgaaaagcc tattgaaaat
     1681 gatgctggta ttatcaaaca tgcaagtact gccacaaaat atttaaatcc attgaataca
     1741 ataaacccgg tattttcgtt ttttctcctt tttataaatt tatttataag cagtgatata
     1801 aatattaata taataggcaa caaaagcaaa tataccaaat ctatcgattt actttccacg
     1861 tacctgacga agtacaaata atcaatcctg tatatattat taacaaaata atcgcttaaa
     1921 acatccgcgc tgaaccctaa taccaggttt tccaatattg aaaatataaa cattataacc
     1981 aacagtatta tcaggggaag gcaaaagtta aatattgtca ttgctcctgc catcaaggta
     2041 tttcctgaca atattgacga caaagacgct aacagtatta ttgccgaaag ccagggaata
     2101 agcattttaa acactttaag tacttcatag tccagcccct gaaaaattac cgacaagaca
     2161 ataagagcca gtaaataaac tgtaaaaact gcatttgtca ctatgttaat aattatctgc
     2221 ttgcctaagg tcacaggctt tgagctgtaa tatgttaact ttgattcatt gtggaaaaaa
     2281 ctgttatcaa ccagacagtt tatcaaaacc aaaacaaaaa gctcaactat accaaacatt
     2341 ataaaaaatt cttcatctac tctcaaaata tatggtatta aaagtgttgt taaaaaaatt
     2401 atagccgttg acagaagtac ccaatttttc ttttccttaa ttgaatatat taatagcggc
     2461 ataaagttat ttttcttttc cataacccac acctcctaat tctgtaataa atatttcttc
     2521 aagactcatt gacagcttat cgaacaacaa aagagtatat ttgctttcca aatatttttt
     2581 aagctcatct atgtttcctg ttaaagttgc aataaatacg ctgccgattt tcacaacctg
     2641 aagcacattg aattctttta gcttatcagg gtcaaattca ccctccactg caaattgaac
     2701 tcttttaata tcctctttca ttttttcaag gttttcttct cttaatattc tgccttcatg
     2761 cattattcct actctgtcgc atatattatc caattccttc aggtcgtggg aagaaataac
     2821 gacagtcatg ttattatcaa ttatttcttt tataagaaca tcccaaaact ttcttttaat
     2881 tactgcatct aatccatcca caatttcatc aagcaagagc agtctaggct gagctgctat
     2941 tgacaaaaca aatgctgcct gctttttttg accttttgac attttgttta atactttatt
     3001 tttatctatg tcaaagtatt ttaacaaata ttcaaatttt tcttcagaca tattatacat
     3061 cagcttttca taatggaata atttgttcaa tgtatactga tagggaaagt acagcgtgtc
     3121 ctgtacaaaa tagaattctt taataatgtc cgagccttcc actacctctt ccccgttaaa
     3181 agatatatgg ccttcatctt gtttgtagac tttcattata tgttttaata tagttgtttt
     3241 tccgcaaccg ttagagccaa ctaaaccata aatccggcct ttctcggcat ttatgtttat
     3301 attctttaaa acctggaaat ctccgaattt tttgcttaaa ttattaatcg ttaacatcat
     3361 ggcctccttt tgtgcctgga gttaaaatat ctctgaccat ttcaattatt ctttcattcc
     3421 ttaaaccaag atcctttaag gatttcacta ccttttgtaa ttcatccttc atattcttta
     3481 aatgtagatt ccttaattca tcagcatctt ttacaaagtt tcccacgccc ttaatactgt
     3541 atatataatt gtcttcttcc aaatatttat aagctttttg gatagtattg ggattgattc
     3601 taatcattga tgccaattct cttactgaag gcaatttttc atcaggtttt aaggatttag
     3661 aaaatatcag ccttactatt tcatcataaa tttgttcgta aatagctttt ggattatcat
     3721 tatcaattcg aatcaatact acacccccaa tcatgcattg acgcaagcaa cctttttact
     3781 aacatattca aacaatcgac cctcatattc cggtataagg ttaaaattat tgtagtcatc
     3841 cggaatgata agtgatattt ccttattaaa gggtatcact ccttttaaca aataagatac
     3901 aaaacttgag cagacaaaat gatattctct ttgatagggt atgttaaagc gaattaaaaa
     3961 aacattcaaa aaactatacc tgtacttatg gggtttagca agaaaatcat taagccgctt
     4021 gattataata tcataatctt catcagttac ttcaaattta tatattttac atttagtttc
     4081 aggatgcatt ttaaacacgc cgctgtttat gtcttccttt ataaatcctc ccactaaggg
     4141 aaaccatgta attttccttc caaaactgaa catagtagag caatcatttt caaaggatat
     4201 cgaaatatga ttatatggtt tttttgaaaa ggcctttagt gttctacata caactgtatt
     4261 tgtatgagtt aaaactattt ttat
//
LOCUS       DAOSOW010000123         4281 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_20639_length_4281_cov_4.238523, whole genome shotgun sequence.
ACCESSION   DAOSOW010000123 DAOSOW010000000
VERSION     DAOSOW010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20639_length_4281_cov_4.238523"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1616)
                     /locus_tag="PLD67_04300"
     CDS             complement(93..1616)
                     /locus_tag="PLD67_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT21774.1"
                     /translation="MEYPRRSYWHDTAEGIKKPAVENADLKNKKFDILIIGAGITGLT
                     TAYLLKDCGFNIGIIEATKSGYGVTGYTTAKITIQHDLFYDYLINSFSMEEAKQYLKA
                     NEEGIRLIKRIINENNIKCDYKEQTAYVYAASVNEVEDLKKELKAYEKLGIDGFYTDT
                     VPIPNKAYGAIGVRNQGQFNPLKYLYSLYNILNQSNCQIYENVRALNIVPHDEHVDVE
                     TESGTIHADKVIVASHYPFDNSFGLYFLRLYQEKEYVIAARTNEPPFEGMYINNNDPT
                     YSLRYQFSDKENLLILAGGNHRVGEKDDENESYNELERFLNSNFKNPQIVSKWSTQDC
                     MTYDKIPYIGRYTDSIDNLYVATGFKKWGMSHSGAAALILKNKILCIEDDYSGIFNPS
                     RFTPVQSSKEFVSSVKSIALGFLNRLEEDLEELSKVNPGEGKIVDYDGKKVGVYRNEA
                     GEYFCINPVCSHLKCALAFNEAEKTWDCPCHGSRFDIKGNILEGPAVLPIDKIKIKK"
     misc_binding    1736..1949
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2047..>4281
                     /gene="ileS"
                     /locus_tag="PLD67_04305"
     CDS             2047..>4281
                     /gene="ileS"
                     /locus_tag="PLD67_04305"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOT21775.1"
                     /translation="MKLFKDISKADYKNNEHEVSKYWEEINLLEQSIETRKNKPSFVF
                     YEGPPTANGRPGIHHVISRTLKDSVCRYKTMQGYQVKRKAGWDTHGLPVEIEVEKQLN
                     LSTKQEIEEYGIDEFNKKCRESVFTYEALWREMTERMAYLIDLDNPYITLENDYIESE
                     WWILDKVFKEGLIYEGHKILPYCPRCGTGLASHEVAQGYEEIKTITAYAKFKKKDEDA
                     YFLAWTTTPWTLLSNVTLTVHPDVDYVKIRTKDKENVKGETYILAKTLVDKVINEEYE
                     VLDEKKGSELEFVEYEQLLPYVEVPAGKKAFFVTLADYVTVEDGTGIVHTAPAFGEDD
                     YNTGLKYNLPVLNPVDDTGRFRGTPWSEMFVMDADQPILKWLKENGKLYKKELVLHNY
                     PHCWRCHTPLLYYARPSWYIQMTKLKDLLVANNNTVNWYPDYVGEKRFGNWLENVNDW
                     AISRSRYWGTPLPIWRCECGHIESIGSRKELVEKAVEDIDESIELHRPYVDEVHIVCP
                     ECGKHMTRVKDVIDCWFDSGAMPFAQWHYPFENKERFDELFPADFINEGIDQTRGWFY
                     SLIAVSTLVTGKSPYKNVLVNDLVLDKYGKKMSKSRGNTVEPMDLFDRYGVDALRWYL
                     LYVSPVWTPTRFDEDGLKEVLSKFIGTIKNVYTFFALYANTDSIKPQDFFVEYKDRPE
                     LDRWIVSKYNNLVKYVTESMDVYDHNKSVRAIQNFVNEDLSNWYIRRSRRRFWETGLT
                     EDKKA"
BASE COUNT         1385 a          702 c          819 g         1375 t
ORIGIN      
        1 tgaaggaaat tatacggagg atgtccatga gatatatggg ttggagaatt gttaaatttt
       61 atgacaatac ccccgtcccc gtccccttgt cattattttt tgattttaat tttgtcaatt
      121 ggaagcacgg caggaccttc caaaatattt ccctttatgt caaacctgga gccgtggcaa
      181 gggcaatccc atgttttctc ggcttcatta aatgcaagcg cacattttaa gtgtgagcaa
      241 acaggattga tgcaaaaata ttctcctgct tcatttctat atactccaac ctttttaccg
      301 tcatagtcaa ctatttttcc ttcgccgggg ttaacttttg aaagttcttc caaatcttct
      361 tctaatctgt tcaaaaatcc taaggcaata cttttcaccg aagatacaaa ttcttttgat
      421 gattgaacgg gagtaaatct agaaggatta aaaatacctg aataatcatc ttcaatgcag
      481 agtattttgt tttttaaaat taatgcagca gcaccggaat gggacattcc ccatttttta
      541 aatccggttg ctacgtataa attatctata ctgtctgtat atctccctat atacggaatt
      601 ttatcgtagg tcatgcagtc ttgagtagac cattttgaaa caatttgcgg atttttaaaa
      661 ttgctgttca aaaacctttc caattcatta tatgattcgt tttcatcatc cttttcaccg
      721 acgcgatgat tgcctccagc taatatcagc agattctcct tgtctgaaaa ctggtatctc
      781 aaagaataag tagggtcatt attgttaatg tacattcctt cgaagggtgg ttcatttgtt
      841 cttgctgcaa taacatattc tttttcctga tacagtctca aaaaataaag tccgaagcta
      901 ttgtcaaagg gataatgtga tgcaactatt accttgtctg catgaattgt tccactttct
      961 gtttctacgt ctacatgctc gtcgtgaggt acaatgttta atgctctaac attttcatat
     1021 atttgacaat tgctttgatt taaaatattg taaaggctgt ataaatattt taaaggattg
     1081 aactgacctt ggtttcttac gcctattgcg ccgtaagctt tatttggaat aggaacagta
     1141 tctgtataaa atccgtctat acctaatttt tcataggctt ttaactcttt tttcaaatct
     1201 tcaacttcat taacggatgc tgcataaaca tatgcggtct gttctttgta gtcgcattta
     1261 atgttgtttt cgttaataat tctttttatt aatcttattc cttcttcatt ggcttttaaa
     1321 tattgctttg cttcttccat gctgaagctg tttattaaat agtcgtaaaa caaatcgtgc
     1381 tgtatagtaa tttttgctgt agtatagcct gtaacgccgt atccggattt tgtggcttca
     1441 ataattccaa tgttgaaccc gcagtctttt aataaatatg cggtagtcag tcccgtaatc
     1501 ccggcaccaa taattaaaat gtcgaatttt ttgtttttta aatccgcatt ttctacagct
     1561 ggttttttta ttccttcagc tgtatcatgc caatatgaac gtcttggata ttccatagta
     1621 acctcttttc ataatatttt tcaataatat tagtgttatt gtttcataaa tatttcaatt
     1681 tattccttga ctttgttcat attttcatat atcataatac agaataaaat caatatcaaa
     1741 aacagtgatt ggaagagtaa ttgcagtagg attcaaagag atataatcga ttggtgtgag
     1801 attataaatc tgaatgcaac gaagatcatc caggagttta atttctgaaa taccagtaag
     1861 aaattacgta caaatgcgtt aaattttcga gtgacatatg ctattattgt atatgtaatt
     1921 tgggtggtac cgcgaaattc gtcccatgtc tattgacatg ggacttttta atggcaaaca
     1981 aattggtgat ggagcctgtc ctgacatcaa ttaaaatata atacgattaa catataggag
     2041 ggaaatatga aattatttaa agatatatca aaagctgatt ataaaaataa tgagcatgaa
     2101 gtatcgaaat actgggaaga aataaaccta ttggaacaaa gcattgaaac aagaaaaaac
     2161 aaaccatcat ttgtttttta tgaaggtcct cccacagcca acgggagacc cggaatacat
     2221 cacgttattt caaggacatt gaaagactct gtctgcagat acaagactat gcaaggatat
     2281 caggttaaaa gaaaagcagg gtgggacact cacggacttc ctgttgaaat tgaagttgaa
     2341 aaacaattaa acttaagtac caaacaggaa atcgaagaat acggaattga cgaatttaat
     2401 aaaaagtgcc gtgaatcggt atttacctat gaagcattat ggcgtgaaat gactgagcgt
     2461 atggcttatt tgattgattt ggataatcct tatattactc ttgaaaatga ctacattgaa
     2521 tccgaatggt ggatattgga taaagttttc aaggagggat taatatatga aggacacaaa
     2581 attcttccgt attgtccaag gtgcggaaca ggtcttgctt ctcacgaggt agcacaaggc
     2641 tatgaagaaa ttaaaacaat aactgcctat gcgaaattta aaaagaagga tgaagatgca
     2701 tattttcttg catggaccac aacgccatgg actttattat ccaatgttac tttaacggta
     2761 catcctgatg ttgactatgt taaaataaga accaaagata aagaaaatgt aaaaggtgaa
     2821 acatatattc ttgcaaaaac cttagtggat aaagtaataa atgaagaata tgaagttttg
     2881 gatgaaaaaa aaggcagcga attggagttt gttgaatatg aacagctctt gccttatgtt
     2941 gaagttcctg caggaaaaaa agcattcttt gtaactttgg cagattatgt tactgttgaa
     3001 gacggtaccg gtattgttca cactgcacct gcttttggtg aagatgatta caacacggga
     3061 ttaaaataca atttgcctgt tttaaatccg gttgatgata caggaaggtt cagaggtaca
     3121 ccttggtcag aaatgtttgt aatggatgca gaccagccga tattaaaatg gttgaaggaa
     3181 aacgggaagt tatacaaaaa agaactggtt ctacacaatt atccgcactg ctggagatgc
     3241 cacacaccgc tgctttacta tgcaagacca agctggtata ttcaaatgac aaaattaaag
     3301 gatttgcttg ttgcaaataa caacacggtt aattggtatc cagattatgt cggtgaaaag
     3361 cgttttggca actggcttga aaatgttaat gactgggcta tttcgagaag ccgttattgg
     3421 ggaacacctc ttccgatttg gagatgcgaa tgcggtcata tcgaaagtat cggttcaaga
     3481 aaagaattag ttgaaaaagc agttgaagat atagatgaat caattgagct tcacagacca
     3541 tacgttgatg aggtgcatat tgtttgtcct gaatgcggaa agcatatgac aagagtaaaa
     3601 gacgttatcg actgctggtt cgattcaggt gccatgcctt ttgcacaatg gcattatccg
     3661 tttgaaaaca aagaaagatt tgatgaattg ttccctgctg attttattaa tgaaggtatt
     3721 gaccagacca gaggatggtt ctattcatta attgccgtat cgaccctggt tacgggtaag
     3781 tcaccatata agaatgtatt ggtaaacgac ttggttcttg ataagtatgg aaagaaaatg
     3841 tcaaaatcaa gaggcaatac agtagagcct atggaccttt tcgatagata cggtgttgac
     3901 gcactaagat ggtacctctt gtacgtgtca cctgtatgga cacctactcg ttttgacgaa
     3961 gacgggttaa aagaggtact gagcaaattt ataggaacaa ttaaaaatgt atatactttc
     4021 tttgctttat atgcaaacac tgattcaatc aaaccgcaag acttctttgt tgaatacaag
     4081 gatagaccgg aactggacag atggattgta tcaaaatata acaacctggt aaaatatgtg
     4141 acagaaagta tggatgttta tgaccataac aaatctgtaa gagcaattca gaattttgtt
     4201 aatgaagatt tgtcaaactg gtacatcaga cgttcaagaa gaagattctg ggagactgga
     4261 cttactgaag ataaaaaggc g
//
LOCUS       DAOSOW010000124         4278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_20664_length_4278_cov_2.991949, whole genome shotgun sequence.
ACCESSION   DAOSOW010000124 DAOSOW010000000
VERSION     DAOSOW010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20664_length_4278_cov_2.991949"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1570
                     /locus_tag="PLD67_04310"
     CDS             <1..1570
                     /locus_tag="PLD67_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR01813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HOT21776.1"
                     /translation="VALEYVSQRIIEGQTTNVEAVSGATISSSSLMALVRKALEEAGA
                     TKDQFSEEYKIARDIKTEREAEIVIIGAGGTGLAAAVTAGQEGASVIVLETNGFAGGN
                     LLVSGGVYNSPEPEMQAKQGIEDSPELFVQQTLAGGDNIANPELVRILAYNALDGLNW
                     LKELGNEFEDQVIQAPGALHPRSHNTTAPLGSGIIKTYLEHIDKMDNVEILYETKGES
                     LIQEDGRVVGVIAKNPDGSKLTVKANKGVIIATGGFSKNAQLVMEYRNAEKWPLLDEN
                     SVSTNMDSIRGEGIIMAREIGADVVDMDQMQFLYLGQPKTGLLSGVFNVSAEVTVFVN
                     KEGKRFVAEDARRDVISSAVFAQEDGMMYMLHSAESLSDPANQLNIDGIPMKELLDIG
                     AYGWKQGDTLEELAKEIGVPAENLVQTIAEYNEAVDTKNDPFGRTLLNVKMEKGPFYA
                     IPRVPALHHTMGGLRIDTLARVLDTKGEPIPGLYAGGEVTGGIHGGNRLGGNAVTDTV
                     VFGRLAAQSAVAGK"
     gene            complement(1705..2823)
                     /locus_tag="PLD67_04315"
     CDS             complement(1705..2823)
                     /locus_tag="PLD67_04315"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT21777.1"
                     /translation="MKNINVGMIGFGYMGKMHTMCYDIMKYYYNTDVNVNMYAVSKTS
                     KTSFPIKFEKIYSDYKSLINDENVDVIDICVPNFLHKEILIEAIKAGKHIYCEKPLAA
                     DIESANEVMEAYKKYNYNKTSRITFEYRFVPAIMRAKQIIDEGKIGNIIHFNFKYYGS
                     EFLDPNRPISWQSTKEKSGGGVLYALGTHAIDLIHYLIGDIDEVYADKRTHFKKRPLL
                     GTDKTVDVEIEDILNVQLYSGDKMGTLLLSQVAAGSGIDLTFEIYGEKGAIKFTHENP
                     NVIYYFNNEDEKSPIGGLSGFKAIETTQKYGKAASFPPPRVNIAWTRYHIASIFDFID
                     AVANNTKAYPDIQDGYKVQLVTDAIYKSAEIKKSITVK"
     gene            2990..3754
                     /locus_tag="PLD67_04320"
     CDS             2990..3754
                     /locus_tag="PLD67_04320"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018782910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HOT21778.1"
                     /translation="MKLKGKTAVVTGASSGMGREIAYYFAKEGANVVAVARRAERLNE
                     LVEQTKDFEGRVVAYSADITNSQKVNEMIDEAIKQFGRMDILVNNAGIMDDMSAVGDV
                     NDEMFDKVFNLNVKTILYSMRKAVKHFEEIGGGVIINISSIAGLYGGAAGATYTASKH
                     AVVGLTKNTAFMYALKNIRCNAICPGAVATEIGNSEFMKNINMEGVQRTGIGMPLNPR
                     TGEPSEIAKVAVFLASDDASFVNGQCIAVDGGWRAY"
     gene            3917..>4278
                     /locus_tag="PLD67_04325"
     CDS             3917..>4278
                     /locus_tag="PLD67_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015958138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOT21779.1"
                     /translation="MRSAEQELYNILEQLNIKYKRYEHKPVYTVEEADNLDIDIPGQH
                     CKNLFLRNKKGNIHYLVIADSQKHIDLKLLSKEIGSSGLSFASEERLYKYLGLKAGSV
                     NPFGLINDIERNVIVLVD"
BASE COUNT         1417 a          669 c          871 g         1321 t
ORIGIN      
        1 agttgcattg gaatatgttt ctcaacgtat tattgaaggt caaaccacca atgtagaagc
       61 agtatcaggg gctaccattt cttcatcaag tttgatggca ttggttagaa aagcattgga
      121 agaagcagga gctacaaaag accaattttc agaagaatat aaaatagctc gtgacattaa
      181 aacggagcgt gaggctgaaa tagttattat tggagcaggc ggaaccggtt tggctgctgc
      241 cgttactgca ggtcaagaag gtgcctcggt tatagttctt gaaactaacg gattcgcagg
      301 cggcaatctc ttagtcagcg gaggtgtata taattcgcct gaacctgaaa tgcaagcaaa
      361 acaaggtata gaagactcac cggaactatt tgtgcagcaa actttggctg gcggggataa
      421 cattgcaaat ccggaacttg taaggatatt agcatataat gctcttgatg ggttaaactg
      481 gctaaaagaa ttaggcaacg aatttgaaga tcaggtaata caagcgccgg gtgctttgca
      541 tcctcgttcg cacaacacga ctgcaccgtt aggatcaggt ataattaaaa cctacctgga
      601 acatattgat aagatggata atgtagaaat tctatatgag actaaaggag aaagcttaat
      661 tcaagaagac ggccgagttg tcggcgttat tgcaaaaaat ccggatggct ctaaattgac
      721 ggtaaaagca aataaaggtg ttataattgc aacaggtggt ttttctaaga atgctcaatt
      781 ggttatggaa tatcgaaatg cagaaaaatg gccattactg gatgaaaatt ctgtatcaac
      841 aaatatggac agtatccgtg gggaaggaat tatcatggca cgagaaatcg gagctgacgt
      901 tgttgatatg gatcaaatgc agtttttata cttaggacag ccaaagacgg ggctattatc
      961 aggcgtattt aatgtttcag cagaagtgac tgttttcgtt aataaagaag gcaagagatt
     1021 tgtagcagaa gatgctcgtc gagatgtaat cagttcggcc gtattcgccc aagaagacgg
     1081 aatgatgtat atgcttcata gtgcagaatc attaagcgac ccggcaaatc aactcaatat
     1141 tgacggtatt ccgatgaagg aattgttgga tatcggagct tatggctgga aacaaggcga
     1201 tacattggag gaacttgcta aggaaatagg agtacctgct gaaaatcttg tacaaacaat
     1261 agcagaatac aacgaagcag ttgatactaa gaatgatcct ttcggaagaa cattattaaa
     1321 tgtaaaaatg gagaaaggac ccttctatgc aattccgaga gtgcccgctc tgcaccacac
     1381 tatgggcgga ttgcgcatcg ataccttggc tcgtgtatta gatactaagg gagaacctat
     1441 cccgggatta tatgccggcg gtgaagtaac cggtggaatc cacggcggca atagattagg
     1501 cggtaatgca gtaactgata cggttgtatt tggtcgtctt gcagcacaat cagcagttgc
     1561 cggaaaatga gataaaaaaa gtttcacata atatcctcaa tctatacaga ttcaatgaaa
     1621 ggctgtaaat aacattattt aagaacagac ccgggactat cccaggtcta ttctaatatt
     1681 atattaatat tgaagagtaa ctttttattt aactgttatt gatttcttta tttcagctga
     1741 tttatatata gcatcggtta ccagctgtac cttatatcca tcttgtatat caggatatgc
     1801 ttttgtatta ttggcaacgg catcaataaa atcaaatatg cttgctatat ggtatcttgt
     1861 ccatgcaata tttactcttg gaggcgggaa agaagcagct ttgccatatt tttgagttgt
     1921 ttctatagcc ttaaaaccgc ttagaccgcc tataggggat ttttcatctt cattattaaa
     1981 atagtatatg acatttggat tttcatgagt aaatttaatg gctccttttt ctccgtatat
     2041 ttcaaatgtt aaatctattc ctgagcctgc tgctacttga gaaagaagca gtgttcccat
     2101 tttatctccg ctataaagct gaacattaag tatatcttct atttctacat ctaccgtttt
     2161 gtccgttccc aataaaggtc ttttcttgaa atgggttctt ttatcagcat atacttcgtc
     2221 aatatctcca atcaagtagt ggattaaatc aattgcatga gttcctaatg cgtataaaac
     2281 tccgccgccg gatttttctt ttgttgactg ccagctgata ggtctgttgg ggtccaggaa
     2341 ttcacttccg taatacttaa aattaaagtg tattatattg cctattttac cttcatctat
     2401 tatctgtttt gccctcatta tggcagggac gaatctatac tcaaaagtaa ttctgcttgt
     2461 tttgttataa ttatattttt tatatgcctc cattacctca tttgctgatt caatatcagc
     2521 ggctaaaggt ttttcgcaat atatatgctt accggcctta attgcttcaa tcaaaatttc
     2581 tttatgtaaa aaattcggta cacaaatatc tataacatct acattttcat catttattag
     2641 gctcttgtaa tccgaataaa tcttttcgaa ttttatggga aaacttgttt tacttgtctt
     2701 tgaaacagca tacatattta cattcacgtc agtattataa taatatttca taatatcata
     2761 gcacattgtg tgcatctttc ccatgtaacc aaatccaatc atccctacat taatattttt
     2821 catccttttc tcctccatat agttataaat ttcaacgcac gctttaaaaa tattatacaa
     2881 acgattgcag aaataatcaa gggattcatc taattgaaaa ataatagttt cggattatat
     2941 tagcagggta taaataatgt atctcaatat taaaatataa ggaggtttta tgaagttaaa
     3001 agggaagact gcagtcgtaa caggcgcaag ttcaggaatg ggaagagaaa ttgcatatta
     3061 ttttgccaag gaaggtgcta atgttgtagc agtagcacgt cgtgccgaaa ggttgaatga
     3121 attggttgaa caaactaaag attttgaagg tagggttgta gcatattctg cagatataac
     3181 aaatagtcaa aaagtaaatg agatgattga tgaggcaatt aaacagtttg gcagaatgga
     3241 tattctggtt aacaatgcag gcattatgga tgatatgagt gcggttggcg atgtaaatga
     3301 tgaaatgttt gataaggtat ttaatttaaa tgtaaaaacc atattatatt ctatgagaaa
     3361 ggctgtaaaa cattttgaag aaattggcgg aggagtaata attaatatct cctctattgc
     3421 cggattgtac ggtggtgctg caggagctac atatacggca agcaaacatg cagtggtagg
     3481 tttaaccaaa aatacggcat ttatgtatgc tttgaagaat ataaggtgca acgcaatatg
     3541 tcccggagcc gtagcaacgg aaataggcaa ttctgaattt atgaagaata ttaatatgga
     3601 aggtgtacaa cgaacgggaa tagggatgcc gcttaatcct cgaacaggtg aaccaagtga
     3661 aattgcaaaa gtcgcagtgt ttttagccag cgatgatgca tcgtttgtaa atggacaatg
     3721 tattgccgtt gatggcggct ggagggctta ctaaatttat ttgacaagat tgctgcaaaa
     3781 aattacagat gaaaatgtat atcattataa taaaagtaat attcaaatat ttcttgaata
     3841 ttgcttttat tttaatatat gttattatag tttaagaaaa acagatgaaa tatatatttg
     3901 gaagggattg aaaatgatta ggtcagctga acaagagtta tataatatat tagaacaact
     3961 aaatattaaa tataaaagat atgagcataa acctgtatat actgtcgaag aagcagacaa
     4021 tttggatatt gatattccgg gacaacactg caaaaattta tttttaagaa ataaaaaagg
     4081 gaatattcat tatcttgtta ttgcagattc acaaaaacat atagatttga aattattatc
     4141 gaaagaaata gggtcttcag gtctatcctt tgcatccgaa gaaagattat ataaatattt
     4201 gggacttaaa gctggctcag taaatccttt tggtttaata aatgacatag aaagaaatgt
     4261 aatagtttta gtcgatag
//
LOCUS       DAOSOW010000125         4264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_20766_length_4264_cov_3.504158, whole genome shotgun sequence.
ACCESSION   DAOSOW010000125 DAOSOW010000000
VERSION     DAOSOW010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20766_length_4264_cov_3.504158"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PLD67_04330"
     CDS             complement(<1..734)
                     /locus_tag="PLD67_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT21780.1"
                     /translation="MNKPVLSAHFESRVPSDVRLSQMKFAERKNKPEAVINVGIGNVS
                     LPTNPAMQKRMFNLDAPESPFNKGVIRYTATNGFAETQDAFKNILKCQGFDTGKLSVI
                     VTDGGSTGMELLIIGVCGPAGTDEKPLMMIDPAYTNYISFAERLGRKTITVKRHLNED
                     GNFSLPEIDKIEETIKTYNPGALLVVPYDNPTGQFYDKETLISLAELCVKYNMWMASD
                     EAYRELFYVEGSDLVSIWGITDKDVPG"
     gene            complement(985..2124)
                     /locus_tag="PLD67_04335"
     CDS             complement(985..2124)
                     /locus_tag="PLD67_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOT21781.1"
                     /translation="MKNKKLVMIPGPTPTVRTINDQMGRETVAFGDPVFVKDFSELIA
                     DLKEMWRVDGECFVVAGSGTMAMEMAVANVTKRDDNILIVSNGFFGDRFIEICNRKGL
                     NVDVLSAEWGDVVSPEAIEEKLKEKNYAAITVTHVDTSTGARAPIEKIGEVIKKFPDT
                     VYIVDGVAASAGEREYVDDMNIDILFTGSQKAFGVAPGLAIVWAGKKALARRKSLGTI
                     PEYFIDFEKWIPIMNDPSKYYATPAVNMVWALKESVRLIKEEGLEERFERHAKVSNAF
                     NDSLEAMGFKLLAKKEYRCNTLSTVLYPEGINDAQFRKVLAEEGAVVAGGLAAYAGKM
                     FRIGHMGNIDDHTVYSTIAAIERTLIRLGHKYEVGIGLKTLLEKM"
     gene            complement(2193..3089)
                     /locus_tag="PLD67_04340"
     CDS             complement(2193..3089)
                     /locus_tag="PLD67_04340"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOT21782.1"
                     /translation="MAYFKIKGAIPPMITPFNENDEVDFEKFAKNIQDWNNYDLAGYL
                     VLGSNSETVYLTEDEKLKLVELTVKYRKSGKLVIAGTGMESTRATIDLTNKAADLGAD
                     CALLVTPFYYGGKMNDEALINYYTDVADNVKIPIMIYNVTKFTHINISARAVGVLCKH
                     PNIIGMKDSSGNVPQLIEFKKAVGNAEFNLMVGTASIWYPALTLGVEAAIMALANCCP
                     GECVDVQKEFEAGNPEKAREIYERVFPVNAAVTGTFGIAGLKYACDILGFNGGYVRKP
                     LLQIKEDDKVKLEAILKDANLI"
     gene            complement(3201..4157)
                     /locus_tag="PLD67_04345"
     CDS             complement(3201..4157)
                     /locus_tag="PLD67_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HOT21783.1"
                     /translation="MKIVILDGYTENPGDLSWEGFENLGELTVYDRTPVEKTIERIGD
                     ADIVLVNKAPISRETLEACKNIKYIGVLATGYNIVDIEAAKERGIPVSNIPTYGTDAV
                     GQFAIALLLEICHHIGHHSQAVREGRWEHNDDWCFWDYPLIELSGKTMGIIGLGRIGK
                     TTGKIAKALGMKVIAYDEYQNDEGRQIVEYVDLDNLFKLSDVICLHCPLFPSTQGIIN
                     KDTIAKMKDGVIIINNSRGQLIVEQDLADALNSGKVYAAGVDVVSTEPIKSDNPLLSA
                     KNCIITPHISWAPKESRQRLMDIAVENLKAFLKGNPINVVNK"
BASE COUNT         1200 a          922 c          615 g         1527 t
ORIGIN      
        1 cccggcacat ctttatctgt aattccccag atacttacta aatcgcttcc ttcaacatag
       61 aataattctc gataagcttc atcacttgcc atccacatat tatatttaac gcatagctct
      121 gcaagtgaaa ttaatgtttc tttgtcataa aactgccctg tggggttgtc ataaggtaca
      181 accagcaatg cgccaggatt gtaagtcttt attgtttctt ctattttatc gatttccgga
      241 agactgaaat ttccatcttc atttaaatgt cgtttaacag taatagtctt acgacctaat
      301 ctttcagcaa aagatatgta gtttgtatat gcagggtcaa tcatcatcag tggtttttca
      361 tcagtgcctg caggtccgca aacacctatt attaataatt ccattccggt tgaaccgccg
      421 tctgtaacta taacgctcaa tttaccggtg tcaaatcctt ggcattttaa aatgttttta
      481 aatgcatcct gtgtttctgc aaatccgttg gtagcggtgt atctgattac acccttatta
      541 aacgggcttt caggagcatc taagttaaac attctttttt gcatagccgg atttgtaggc
      601 aacgaaacat taccgattcc tacattgatt acagcttcag gtttattctt acgctctgca
      661 aacttcattt gtgacagtct aacatctgaa ggtactcttg attcaaaatg tgctgacaac
      721 actggtttat tcatttataa aaccctccta ttaatatatt gttatatata aatttaatag
      781 tacctattaa atataattat aacgaacttt aagttttttg tccatacatt tagcttaatt
      841 atatggtatt taatatttat tgtttgttac tgtttcgcca aatattcatt tactgctatt
      901 atgtgtgaac tgtaatcggt aaagagtatt tttttattat ttgcaaaaac aaagcagcta
      961 atcgatagct gctttatata ttaattacat tttttcgagc aatgttttta aacctattcc
     1021 tacttcatac ttgtgtccta atctaatcaa tgttctttcg atagctgcaa ttgtcgaata
     1081 aaccgtgtgg tcatcaatat tacccatatg tcctattcta aacattttgc ctgcatatgc
     1141 ggcaagtcct cctgctacca cggcgccttc ttcagccagt acctttctga actgcgcatc
     1201 atttattcct tcaggataca aaactgttga aagtgtattg catctgtatt ctttttttgc
     1261 caataactta aatcccattg cttctaaaga atcgttgaaa gcattggata cttttgcatg
     1321 tctttcaaat ctttcctcaa gtccttcttc ttttatcagt cttactgatt ctttaagagc
     1381 ccaaaccata tttacggcag gagtagcata atattttgac gggtcgttca tgatcggtat
     1441 ccatttttca aaatcgatga aatattcggg aatagttcct aaggattttc ttcttgcaag
     1501 ggctttcttt cctgcccata ctattgctaa acccggtgca actccaaatg ccttttgaga
     1561 acctgtaaac aagatatcaa tattcatatc atcaacatat tctctttcac ccgctgaagc
     1621 tgcaactccg tcaactatgt atactgtgtc agggaatttc tttataactt cacctatttt
     1681 ttcaattggt gcccgagcac ccgttgaagt atccacatgg gttactgtaa ttgccgcata
     1741 atttttttct ttcaattttt cttcgatagc ttcgggagaa acaacatctc cccattcagc
     1801 agacaataca tctacattaa gtcctttcct gttacagatt tcaataaatc tgtcaccaaa
     1861 aaatccatta gatactatca atatattatc atctcttttt gtaacatttg caactgccat
     1921 ttccatagcc attgtaccgc ttccggctac aacaaagcat tcaccgtcaa ctctccacat
     1981 ttcctttaaa tctgctatta actcgctaaa atccttaaca aaaacaggat ctccaaatgc
     2041 aactgtttct cttcccattt ggtcatttat agttcttact gtgggagtgg gaccgggaat
     2101 cattactaat ttcttgtttt tcatagtatc ctcctatact aatattttat ttttaccttt
     2161 gttatataaa gagactcttt aaagaagttt aattatatta aatttgcatc ttttaagatt
     2221 gcttccagtt taaccttatc atcttccttt atctgtaata aaggtttcct gacatatccg
     2281 ccgttaaatc ctaatatatc gcaagcatat tttaaaccgg caattccaaa agttcctgta
     2341 acagctgcat taaccgggaa tactctttca taaatttctc ttgctttttc gggattgcct
     2401 gcctcaaatt ctttttgaac atccacgcat tcacccggac agcagtttgc tagagccata
     2461 attgctgcct caacacctaa tgtcaaagca gggtaccaaa tagatgcagt accgaccatc
     2521 aaattaaatt cagcattgcc gacagctttt ttgaattcta tcagctgtgg aacattgcct
     2581 gaactatctt tcattcctat aatgtttgga tgtttgcaca agacacctac tgcacgagca
     2641 gaaatattta tatgagtaaa cttagtcaca ttgtaaatca ttataggaat ttttacatta
     2701 tcggcaacat ccgtataata attaatcaaa gcttcatcat tcattttccc tccataataa
     2761 aatggagtga caagaagtgc acagtcggca cctaaatcag ccgccttatt cgttaaatcg
     2821 atggtggctc ttgttgattc catccctgtt cctgcaatca caagtttacc cgattttctg
     2881 tattttactg tgagctctac taacttaagc ttttcatcct ctgttaaata tactgtttca
     2941 ctgttagagc caagaacgag atagccggct aaatcatagt tattccaatc ctgaatattt
     3001 tttgcaaatt tttcaaaatc aacctcatcg ttttcattaa aaggagttat cattggaggt
     3061 attgctccct taatcttaaa atatgccatt tttctctcct attatttttt tattattagc
     3121 tgcaattcgt ttcttcacat taaaatacta caacttttta cagtacatgt caatgaagtg
     3181 aataatcaaa cagttaacct ctacttattt acgacattaa tgggattgcc ctttaaaaat
     3241 gcttttaaat tctcgactgc aatatccatc aggcgctggc ggctttcctt tggagcccaa
     3301 gaaatatgcg gagttattat acaatttttt gcactgagaa gcggattgtc ggattttatc
     3361 ggctctgtag ataccacatc taccccggct gcataaacct ttcctgaatt caatgcatct
     3421 gccaagtcct gctcaacaat taattgacct cttgaattat ttattataat aactccgtcc
     3481 ttcatctttg caattgtatc tttattaata attccttgtg ttgatgggaa caaagggcaa
     3541 tggaggcata taacatcaga caatttaaaa agattgtcca aatctacata ttcaacaatt
     3601 tgccttcctt catcattttg atattcgtca tatgcaatta ccttcatacc caatgccttg
     3661 gctattttgc cggttgtttt accgattcgt ccaagtccta ttattcccat agtttttccg
     3721 gaaagctcaa tcagcggata atcccagaaa caccagtcat cattatgctc ccagcgacct
     3781 tcccttacag cttgagagtg gtgtcctata tgatggcaaa tctccaataa aagtgctatt
     3841 gcaaactgtc cgacggcgtc agtaccataa gtgggaatgt ttgaaacggg aattcccctt
     3901 tctttggcag cttctatatc cacaatatta taaccggttg ccaaaacacc tatgtattta
     3961 atatttttgc atgcttctaa tgtttccctt gatatgggag ctttgtttac taatacaata
     4021 tctgcatctc cgatacgctc tatggttttt tctaccggag ttctgtcata taccgtcagt
     4081 tcacccagat tctcaaatcc ttcccagctt aagtcaccgg ggttttccgt ataaccatct
     4141 aaaataacaa ttttcattcc aaacctcctg tttttatttt gcatccatac tgcttcttga
     4201 ctctttcttt aattatcaaa gaatacaatt gctttgtcaa tagaatttcc aattgttttt
     4261 tatc
//
LOCUS       DAOSOW010000126         4260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_20795_length_4260_cov_4.282996, whole genome shotgun sequence.
ACCESSION   DAOSOW010000126 DAOSOW010000000
VERSION     DAOSOW010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20795_length_4260_cov_4.282996"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1400
                     /locus_tag="PLD67_04350"
     CDS             <1..1400
                     /locus_tag="PLD67_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21784.1"
                     /translation="KRNNNISVAEPFIYDVQATGEKPLNNRPVVVGSGPAGLFCALLL
                     AEKGYRPIILERGKTVKERVKDVEKFWKEGILNYSSNVQFGEGGAGTFSDGKLNTLIK
                     DRSKRGKKVLEEFVEAGAPAEITYINKPHIGTDLLRNVLVNIRKKIISLGGTFRFEEC
                     LTDIIIKDNAVQRIVTESDIIETDILVLAIGHSARDTFEMLNNKLKLTAKPFAIGVRV
                     EHPQKMICEAQYGEMAGNPLLGPADYKFVHKSKNGRSVYTFCMCPGGVVTASASEAGG
                     VVTNGMSFHERDLENANAAVVVQINPEDFGGEENPMAGVEFQRYWERKAFEAGGGNYR
                     APVQLFKDFKEKIVSSSFGEIYPTYRPGCTFANLWDCMPDYVCESLVEGITAFGKFLT
                     NYDRPDALLTGVETRTSSPLRILRDDEMQSNVRGIYPCGEGSGYAGGIMSASIDGLKV
                     AEQILKTYKTINDKE"
     gene            1404..2624
                     /locus_tag="PLD67_04355"
     CDS             1404..2624
                     /locus_tag="PLD67_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOT21785.1"
                     /translation="MKIAVIGGGASGLTAAIAAARNGAEVTICEKLNRVGKKILATGN
                     GRCNYTNMNLSKECYHSNNLNFAEEVMKFFNLDKTLVFFEDLGILPYVDESGKVYPNS
                     LQASSVLDVLRYELKRHKVKEVTDFNVTALRKSKDKFSIIGNDTITADRVILATGGKA
                     SPQLGSDGKGYELAKSLGHEIIEPFPALVQLKLSGKYFKRIAGIKFDGIVKAYAGDRL
                     IREEEGEILFTDYGISGPPILQVSRKVIEELNKKNKPFLNIDMFPGYSKLKLYDILQD
                     RFRRINYKTIEESMIGFINKKLIPVVFYEAGFEDLNKICGKLNKKEIYKIIEILKEWK
                     FEVIGHNSWQQAQSTAGGIKLSEVNPKTLESLKVKGLYFAGEILDVDGDCGGFNLQWA
                     WSSGYTAGYFSSLE"
     gene            2644..>4260
                     /gene="secA"
                     /locus_tag="PLD67_04360"
     CDS             2644..>4260
                     /gene="secA"
                     /locus_tag="PLD67_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOT21786.1"
                     /translation="MKNFFEKIFGSYSEKEIKRIQPIVDKVLSLEKTMETLTDSQLKG
                     KTAEFKQRLANGETKDDILPEAFAVVREAAWRVLGQKHYPVQIIGGIVLHQGRIAEMK
                     TGEGKTLVSTLPVYLNALEGNGVHVVTVNDYLARRDADWMGKVHNFLGLSVGCIVHGI
                     TKEERLSAYRSDITYGTNNEFGFDYLRDNMVIHKEDMVQRDLNFCIIDEVDSILIDEA
                     RTPLIISGEGEKSTDLYEMANSFVLNLKGKIQLPEEKKTKMDYIMGDVEEDDTVDYIV
                     DEKAKNVTLTARGIDKAEKYFGVENLADQENMELTHHINQALKAHNTMKKDIDYIVKD
                     GEVLIVDEFTGRIMYGRRYSNGLHQAIEAKERIEVRKESKTLATITFQNYFRMYKKLS
                     GMTGTAKTEENEFREIYGVDVIEIPTNKPVIREDLQDVVYKGEAGKFKAVINEIIERH
                     QTGQPILVGTISIEKSEILSKLLKKQGIKHEVLNAKQHDKEAEIVAQAGRLGSVTIAT
                     NMAGRGTDIVLGGNPEFIAKHKLKAKGFSDE"
BASE COUNT         1538 a          664 c          976 g         1082 t
ORIGIN      
        1 ccaaaagaaa caacaacatt tccgttgcag agccatttat atatgatgtg caagctacag
       61 gagaaaagcc cttaaacaac aggcctgtag tggtaggaag cggtcccgca gggctttttt
      121 gcgcactgct tcttgcagag aaaggctaca gacctataat tctcgaaaga ggaaagactg
      181 ttaaggaaag agtcaaagat gttgaaaaat tttggaaaga aggcatttta aactacagct
      241 caaacgtgca gtttggagaa ggaggagcag gaacattttc cgacggtaaa cttaacactt
      301 taattaagga caggtcaaaa agaggcaaaa aagtattgga agaatttgtt gaagcaggtg
      361 ccccggcgga aatcacatat attaacaaac ctcatatagg aacagacctt cttagaaatg
      421 ttcttgtaaa tataagaaaa aaaataataa gtctgggtgg aacattcagg tttgaagaat
      481 gtttaacgga cataattatt aaggataatg ctgtccaacg cattgttacc gaaagtgaca
      541 ttattgaaac ggatatattg gtacttgcaa taggacatag cgcaagagat acatttgaaa
      601 tgctgaataa taagttaaaa ctaacagcta aaccatttgc gattggtgtc agggtagagc
      661 accctcaaaa aatgatatgc gaagctcagt acggagaaat ggcagggaat ccactcttag
      721 gtcctgctga ctataaattt gttcataaaa gtaaaaatgg aagaagcgta tatacatttt
      781 gcatgtgccc gggtggagtt gtaactgctt ctgcctccga agccggggga gttgttacaa
      841 acggaatgag ttttcacgaa agggatttgg aaaatgcaaa tgctgccgtg gttgtacaaa
      901 taaatcccga agatttcggc ggcgaagaaa atccaatggc aggtgtagaa ttccaaagat
      961 actgggaaag aaaagccttt gaggcaggcg gaggaaatta tcgggctccg gtgcagctct
     1021 ttaaagattt taaagaaaaa attgtttctt catcttttgg agaaatatat cctacataca
     1081 gaccgggctg tacctttgca aacctgtggg attgcatgcc tgattatgtg tgtgaaagcc
     1141 tggttgaagg aataacagct tttggaaaat ttttaacaaa ttacgacaga ccggatgcac
     1201 tgctaacggg agttgaaaca agaacttcat caccgttaag aattttaagg gatgatgaaa
     1261 tgcaatcaaa tgtaaggggg atttatccct gcggcgaagg ctcgggttat gcaggaggaa
     1321 ttatgtctgc atcaatcgat gggcttaagg tagcggagca aattcttaaa acatacaaaa
     1381 ccattaacga taaggaataa actatgaaaa ttgctgtaat cggcggaggt gcatcaggtt
     1441 tgaccgcagc gatagctgcg gccagaaacg gtgctgaagt aactatctgc gaaaaattga
     1501 acagagtagg caaaaaaatt ctggcaaccg gcaacggcag gtgcaattat accaatatga
     1561 atctgtcaaa ggaatgctac cactctaata atttgaattt tgcagaagaa gttatgaaat
     1621 tttttaatct tgataaaact cttgtctttt ttgaagactt aggaattttg ccttacgttg
     1681 atgaaagcgg caaagtatat ccaaattctc tccaggcttc aagcgtactg gatgttttaa
     1741 gatatgaatt aaaaagacat aaggtcaagg aagttactga ctttaacgta acagccttaa
     1801 ggaaaagcaa agataaattt agtattattg ggaatgatac aattactgca gacagggtta
     1861 ttttggcaac gggaggaaaa gcaagtcctc agttaggctc tgacgggaaa ggatatgaat
     1921 tagccaaatc cttgggacat gaaataatag agccctttcc tgcccttgtt cagttaaaat
     1981 taagcggtaa gtactttaaa agaattgcag gaataaaatt tgacggtatc gtaaaagcct
     2041 atgcaggcga taggcttata agagaagaag aaggggagat actttttacc gattacggca
     2101 tatcaggacc tccgattctg caggtaagcc gaaaggttat tgaggagcta aataagaaaa
     2161 ataaaccttt tttaaacata gatatgtttc ccggatattc aaagcttaag ctttatgata
     2221 ttcttcagga cagattcaga agaataaact ataaaacaat tgaagagtct atgatcggtt
     2281 ttataaacaa aaaattaatt ccggtagttt tttatgaagc aggctttgaa gatttaaata
     2341 agatatgcgg caaattaaac aagaaagaaa tatataaaat aatagagatt ttaaaagagt
     2401 ggaaatttga agttatagga cataattcat ggcagcaggc tcagtcaacg gcaggaggga
     2461 ttaagctttc tgaggtaaat ccaaagacct tggagtcttt aaaggtgaaa ggattgtatt
     2521 tcgccggaga aattcttgat gtagacggag actgcggagg atttaatctt caatgggcat
     2581 ggagttcagg ctacactgcc ggatatttca gctctcttga ataaagtatg gaaggatact
     2641 aatatgaaaa atttttttga aaaaattttt ggttcataca gcgaaaaaga aattaaaaga
     2701 atacaaccta tcgtagataa ggtattatct ttggaaaaga caatggaaac tttgaccgac
     2761 agccagctca agggtaagac tgccgaattc aaacaaaggc ttgcaaatgg agaaacaaag
     2821 gatgatatat tgccggaagc atttgcagta gtaagagaag ccgcatggag ggtattgggt
     2881 caaaagcatt atccggttca aattataggc ggaatagttc ttcatcaggg acgaattgca
     2941 gaaatgaaaa caggagaagg aaaaacactc gtttcaacat tgcctgttta tttgaatgct
     3001 ctcgaaggca acggggtgca tgttgtaacg gtaaatgatt acttggcaag gcgtgacgcc
     3061 gactggatgg gaaaggtgca taatttctta gggctttctg taggctgtat agtccacggc
     3121 attacaaagg aagaaaggct tagtgcatac aggtcggaca ttacctacgg aacaaacaat
     3181 gaattcggtt ttgattattt aagagacaac atggttattc ataaggaaga catggttcaa
     3241 agggatttaa acttctgcat tatagatgag gttgactcta tacttataga tgaagcaaga
     3301 acgcctctta ttatttccgg tgaaggagaa aaatcaaccg acctatatga aatggcaaac
     3361 agctttgtac tcaatttgaa gggaaaaatt caactgcctg aagaaaagaa aacaaagatg
     3421 gactatatta tgggggatgt tgaagaagat gatactgttg attatatagt cgatgaaaag
     3481 gcaaaaaatg ttacccttac tgcaagaggt atcgataaag cagaaaaata tttcggtgtt
     3541 gagaatttag ccgaccagga aaacatggag ctcacccatc atataaatca agccttaaaa
     3601 gcccataata cgatgaagaa agatatcgac tacattgtaa aagacggaga agttctgatt
     3661 gttgatgaat ttaccggacg tataatgtac ggcaggagat attccaacgg acttcatcag
     3721 gcaatcgaag caaaggaaag aatagaagta agaaaagaat caaaaacact tgccaccatt
     3781 acatttcaaa attacttcag aatgtataaa aaactgtccg gtatgacagg aacagcaaag
     3841 acggaagaaa atgaattccg tgaaatttac ggtgtggatg taatagaaat tccaaccaat
     3901 aagcccgtaa taagagaaga tttgcaggat gttgtctaca agggagaagc aggcaaattc
     3961 aaagccgtaa taaacgaaat tattgaaagg caccaaacag gacagcccat tttggttggt
     4021 actatttcta tagaaaaatc agaaatttta agcaagcttt taaaaaagca aggaatcaag
     4081 catgaagtat taaatgcaaa gcagcatgac aaagaagctg aaattgtagc ccaggcagga
     4141 cgtctcggct ctgttacaat tgcaaccaac atggcaggaa gaggaaccga cattgtgctt
     4201 ggaggaaatc cggaatttat tgcaaagcat aaacttaagg caaaaggatt ttccgatgag
//
LOCUS       DAOSOW010000127         4254 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_20831_length_4254_cov_2.820910, whole genome shotgun sequence.
ACCESSION   DAOSOW010000127 DAOSOW010000000
VERSION     DAOSOW010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20831_length_4254_cov_2.820910"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1131)
                     /locus_tag="PLD67_04365"
     CDS             complement(67..1131)
                     /locus_tag="PLD67_04365"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003337838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOT21787.1"
                     /translation="MDLFSKMEEYDYEQLILCQDKSTGLKAIIAIHDTTLGPALGGTR
                     ICEYESDEEAIEDALRLARGMTYKNAAAGLNLGGGKAVIIGDPRKIKSEALFRAFGRF
                     VEGLNGRYITGEDMNATQEDASYINCETEYIVGLETGSGNPSPVTAYGVFKGIQAALN
                     EVYGSDDLTGKTVAIQGLGAVGRRLAERLHEAGAKLYVTTRDKAKIDKAVAELGATAV
                     SLDEIHGVECDVFSPCARGAIINDKTIEQFKCKIIAGAANNQLAESRHGDMLHEKGIL
                     YVPDYIINSGGVINIIDDISGREYNRENAMKNTARIYDSCKKVFEIAKRDNIPTYKAA
                     DIMAEERIAAVRKIKKIYNR"
     gene            1367..2314
                     /locus_tag="PLD67_04370"
     CDS             1367..2314
                     /locus_tag="PLD67_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HOT21788.1"
                     /translation="MKYLSKPFNNGIINLKNRLIMPPMATAKSETNGRITQDLIDYYD
                     EKSKGGYISLIIIEHSYISEDGKASKNQISIAEDSVIDDLRKLSDVIHKNGSKTVMQI
                     NHAGSRALEVGNSIHRGPIIDKDEIKEIIYNFRKAAIRVKEAGFDGVEIHSAHNYLLN
                     QFLSPITNKREDEYGGNIHGRIRIHLDVIKAVREAVGEEFPILIRLGATDDDVEGLTL
                     KDAVEAAKCLEKAGVDMIDVSGGMCRYMPSNSNEQGYFSTQSEEIKKAVNIPVMLTGG
                     ITDPYIADRMLENGKADFIGVGRAILYDSLWAKRAMELQ"
     gene            complement(2395..3570)
                     /locus_tag="PLD67_04375"
     CDS             complement(2395..3570)
                     /locus_tag="PLD67_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOT21789.1"
                     /translation="MNEVVIGLIGSGYAGFLHVNGYKKVYGIPVRIKTIVDVDIEKAK
                     IFAKTHCIENASMNIEDVLNDPEIQIIDIATPPMTHIDIAAKALKAGKHVICEKPLSG
                     YFGQEGDVAPIGKTVPKEKMFKKVMEEIDEFKKVVEESGKLFMYAENYIYCPSVTKAA
                     EIIAHKKSKLLFLKGEESLRGSSSPVAGRWDKTGGGSFIRVGTHPLSGILWLKQVEAK
                     ARGEKINVVSVSADMGNTASMLNQYDRRHLTLETYDVEDFANVTLTFSDNTKAIVIGA
                     DNVLGGTKNYIEIYANDTSLMCNITPANNLQSYFLDEDNLEDVSISEMLPGKTGWNNV
                     FICDEILRGYTFEMQDFVESVAYNRQPKSGIELAYETTKILYAAYQAAYEQKTVFLV"
     gene            3898..>4254
                     /locus_tag="PLD67_04380"
     CDS             3898..>4254
                     /locus_tag="PLD67_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT21790.1"
                     /translation="MSSDLDIQNTIYDTIFEQVPFGIAVSYNCYPMKVSENKNASINA
                     MYEKITGRKREEMIKLGWAAITHPDDLQENLDKYNQLQEGKIDSYSMQKRFIRPDGSI
                     VWVDMTVARLNFEGSRY"
BASE COUNT         1349 a          817 c          720 g         1368 t
ORIGIN      
        1 aatatacaat tttaaaaacc aatctgccaa aattatcggc agattggtta atactaaatt
       61 ttagatttat cggttataga tttttttaat tttgcgaact gctgctattc tctcttctgc
      121 cataatatca gcggccttat atgttggaat attatctctt ttagctatct caaacacctt
      181 cttgcaagaa tcataaattc ttgcggtatt tttcattgca ttttccctat tgtattcacg
      241 tcccgatata tcatcaatta tattaatcac accgccgctg tttataatat aatccggcac
      301 ataaagtatt cctttctcat gcagcatatc accgtgtctt gactcagcta actgattgtt
      361 ggctgcgccg gctatgattt tacatttaaa ttgttctatt gttttatcgt ttattatagc
      421 acctctagca caaggtgaaa atacatcaca ttctacgcca tgtatttcat ccaggcttac
      481 tgctgttgca cccagttctg ctacagcttt atcaattttt gccttatctc ttgtagtaac
      541 gtataattta gcacctgctt catgtaaacg ctcggctaaa cgtctgccga cggcacctag
      601 accttgaatt gctacggttt ttccggttaa atcatcgctg ccgtaaactt catttaaagc
      661 tgcctgtatt cctttaaata ctccataagc ggttacaggt gaaggattgc cgcttccggt
      721 ttctaaacct actatatatt ctgtttcaca attaatatat gaagcatctt cttgtgttgc
      781 attcatatct tctccggtta tgtatctgcc atttaaacct tcgacaaatc tgccgaaagc
      841 tctaaacaat gcttcgcttt ttatcttcct tgggtctcct ataataactg ctttcccgcc
      901 gccaaggttt aatcctgcag ctgcattttt atatgtcata cccctggcta atcttagcgc
      961 atcttcaatc gcttcttcat cagactcata ttcgcaaatt cttgttcctc ctagtgccgg
     1021 acctaatgta gtatcatgaa tagcaataat tgcctttaat ccggtacttt tatcttgaca
     1081 aagtattaat tgttcgtaat catattcttc catcttacta aataaatcca tatagcgttc
     1141 ctcctcttat gacgatatta atttatttat tataacatat atgtcaacaa aaatgcagca
     1201 aacattaaaa gtgcccggca cctcaaaata tgttaatttt attgaaataa cttcgtgcca
     1261 tgatataatg aatacaatca atgaagcaaa ggctttagcc tttgtggtgc cgatggtgcc
     1321 aggcaccaaa ctgaaaggca cagtgataaa aaaggagaag atatctatga aatatttatc
     1381 aaaaccattc aataacggaa taattaattt aaaaaacaga ctgataatgc cgccaatggc
     1441 tactgcaaag tcagaaacca atggcaggat aacccaagac ttaattgatt attatgatga
     1501 aaaatcaaaa ggggggtaca tatcactaat aataattgaa cacagctata tttcggagga
     1561 tgggaaggca tccaaaaacc agatttctat tgctgaagac agtgtgatag atgatttaag
     1621 aaaattatcc gatgttatac ataagaatgg ttcaaaaaca gtgatgcaga ttaaccatgc
     1681 aggaagtcgg gcattagaag ttggtaacag tattcataga ggaccaataa tagacaagga
     1741 tgaaattaaa gaaattatct ataattttag gaaggctgca attcgtgtca aggaagcagg
     1801 ctttgacggc gtagaaatac attctgctca caattatctc ttaaatcagt ttttatctcc
     1861 tataacaaat aagagagaag atgagtatgg cggaaatata catggcagaa taagaattca
     1921 tttagatgtt attaaggcag taagagaggc tgtaggagaa gaatttccca ttttgattcg
     1981 ccttggtgca acagacgatg atgttgaagg attaacgctg aaggatgccg tcgaagcagc
     2041 aaaatgtctc gaaaaagccg gagtggatat gattgatgtt tcaggcggaa tgtgcagata
     2101 tatgcctagt aacagcaatg agcaaggata tttttcaact cagtctgaag aaattaaaaa
     2161 ggccgttaat attcctgtta tgttaacagg tggaataaca gacccatata ttgccgatag
     2221 aatgctcgag aatggaaaag cagattttat cggtgtagga agagctatat tgtatgattc
     2281 tttatgggcg aaaagagcaa tggagctcca ataggggcgt aaaaagtgcc aggcaccaaa
     2341 cgcaaccgtt gcctggcacc actttgttct tgtgtactct atacaaggtg gtatctaaac
     2401 taaaaataca gttttttgct cataggctgc ctgataagct gcataaagta tctttgttgt
     2461 ttcataagcc aattcaattc cggatttcgg ttgcctgtta taagctacac tttctacaaa
     2521 atcctgcatt tcaaaagtat atccacgaag tatctcatcg cagatgaata cattattcca
     2581 gcctgttttt cccggaagca tctcagaaat acttacatct tccaaattat cctcgtctaa
     2641 gaaataggac tgcagattat ttgccggagt tatattacac ataagcgatg tatcatttgc
     2701 atagatttca atataattct tggtgcctcc tagaacattg tcagctccaa ttacaattgc
     2761 cttagtattg tctgaaaaag tcaaagttac atttgcaaaa tcctctacat cataagtctc
     2821 aagggtcaaa tgtcttctat catattgatt aagcatagaa gcagtattac ccatgtcagc
     2881 tgatacactg acaacgttga ttttctcacc tctcgctttg gcttctactt gtttaagcca
     2941 caatattccc gaaagaggat gagttccgac tctaataaat gagccgccac ctgttttatc
     3001 ccatcttccg gcaacaggtg aggatgaacc ccttagactt tcttcgcctt tcaaaaacaa
     3061 taatttgctt tttttatggg caattatttc tgcagctttt gtaacagaag gacaatatat
     3121 gtaattttca gcatacataa aaagtttgcc gctttcttca actactttct taaattcatc
     3181 aatttcttcc attaccttct taaacatctt ttctttagga acggtcttac ctattggagc
     3241 tacatctcct tcttgtccaa aataaccgga taatggtttt tcgcaaataa catgtttgcc
     3301 ggcttttaag gcttttgcag ctatatcaat atgggtcatt ggaggagttg caatgtcaat
     3361 aatttgaatc tccggatcat taaggacatc ttcaatattc atagaagcat tctctatgca
     3421 atgggtttta gcaaaaattt ttgccttttc aatatccacg tctactatag tctttatcct
     3481 aacgggaatt ccatagacct tcttatatcc gtttacatgt aaaaaccctg catatccact
     3541 gccgattaat ccaattacta cttcattcat aaaaatacca cctttcatat cggaatcatt
     3601 accgtatcga ttaatacatt taatttatta tacaattttt aaaaggaaag tcaataaagc
     3661 agtattgttc gtatcgccta cttataagaa gcttctcata tattcacatt atagttgatt
     3721 aataatctgt atcgataaaa tggcaaaagc agtgccacaa aagcggtgcc aggccccttt
     3781 ggttatcaca aattccattc tttcaattta tgttttttgt tgtaaaattt tacatataat
     3841 gatataatta tataaataat accaaattct gattataaca gacaatttgg aggaataatg
     3901 agcagtgatt tagacataca gaacacaata tatgatacta tttttgaaca agttcccttt
     3961 ggcattgccg tttcatacaa ttgctatcca atgaaagtaa gcgaaaacaa aaatgccagc
     4021 attaatgcta tgtatgaaaa gatcacgggc agaaaaagag aagaaatgat aaagcttggt
     4081 tgggccgcaa taacacatcc ggatgacttg caagaaaacc ttgataaata caaccaatta
     4141 caagagggta agattgacag ctattcaatg caaaaacgtt ttataaggcc ggacggttca
     4201 attgtatggg ttgacatgac tgttgccagg cttaattttg aaggatcaag atat
//
LOCUS       DAOSOW010000128         4231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_21000_length_4231_cov_3.252395, whole genome shotgun sequence.
ACCESSION   DAOSOW010000128 DAOSOW010000000
VERSION     DAOSOW010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21000_length_4231_cov_3.252395"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            154..573
                     /locus_tag="PLD67_04385"
     CDS             154..573
                     /locus_tag="PLD67_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21791.1"
                     /translation="MEVDIINHKITEGLSITYQKQTNASDAYVSNTSSIENLVSTPAL
                     LTMIVEASCKMLDSLIPQQCLTVGNRLEISHINPTLVGDTITLILTVEKVDGNKILLD
                     IVGADSNGRICLGKYHKVIVERNKLMENAYKRSKDWQ"
     gene            637..1434
                     /locus_tag="PLD67_04390"
     CDS             637..1434
                     /locus_tag="PLD67_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003240247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HOT21792.1"
                     /translation="MANKYHDFSPKEVRQLIREEKIQEPTSGMCGGYAQANLVILPKK
                     YADDFKLFAEYNPKPCPILEITEPGSYTTTKTADKANIITDIPKYFIYKNGVRVDEVL
                     FAEKYWGDDFVCFLIGCSFSFEEAMIREGIEIRHISMGRNVPMYKTNIEAKPAGIFKG
                     PYVVSMRPMKPEDVDKAYEITGKFPHVHGAPIHHGDPAEIGIKDINKPDYGEAVIIKE
                     GEVPVFWACGVTPQAAIENAKPEIVITHAPGHMFITDILNEDIESIF"
     gene            1738..2133
                     /locus_tag="PLD67_04395"
                     /pseudo
     CDS             1738..2133
                     /locus_tag="PLD67_04395"
                     /inference="COORDINATES: protein motif:HMM:NF020543.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
     gene            2131..2544
                     /gene="ndk"
                     /locus_tag="PLD67_04400"
     CDS             2131..2544
                     /gene="ndk"
                     /locus_tag="PLD67_04400"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOT21793.1"
                     /translation="MSDFLGKERTLVLIKPDAIERKLMGEIISVYEKNFNIIDMKMIK
                     PSVEIAEEHYYEHRDKPFFNELISYIIRGNVCALIIEGKNVIERVRKINGATDPKNAE
                     PDSIRGKFALSKQENSVHGSDSKESAEREIKIWFG"
     gene            2613..3164
                     /locus_tag="PLD67_04405"
     CDS             2613..3164
                     /locus_tag="PLD67_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOT21794.1"
                     /translation="MNKKFEGTKPIIDEKTYIADGAQIIGKVTIKEYSSIWFNTVVRG
                     DVNYIEIGRYTNIQDNSVVHVADDYPAIIGDYVTIGHNATVHACKIEDHCLIGMGAII
                     MDGAVIGRGSIIAAGAVVTNNQKIPPFSVAAGIPAKVIKTVPEEDIIKIHSQALKYKH
                     VWSIRYGIMPDCGGESYDGGEIV"
     gene            3191..3895
                     /locus_tag="PLD67_04410"
     CDS             3191..3895
                     /locus_tag="PLD67_04410"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT21795.1"
                     /translation="MVIKKDFYYDSADKKSKIHAVKWVPDAEIRGVLQIAHGMLEHID
                     RYDCFARYMAENGILTAGNDHLGHGDSVLVEEDRGFFGEGDGNKLVTKDMRKLREILK
                     EEYEGLPYFILGHSMGSFLVRQYISIYNDLNGALLVGTAYQPYLSVKAGLIISKLMAV
                     FKGWRYRSKFINKLTIGGNNKYFEPAKTKSDWLSRDEEAVDAYVKDRKIDFIFTLNAF
                     YYMFKGMLYLYSKEYI"
BASE COUNT         1543 a          627 c          846 g         1215 t
ORIGIN      
        1 tcacaaagag taattgaagc acctgttgaa aaagcggcat atgtgaacat tggacccgta
       61 agaagaatga atatagtaat aaacggagta aattatgtgg tagatgttca agagattcaa
      121 tgaattctaa gatacgatta agcaaatgaa tatatggagg tggacattat aaaccataaa
      181 attaccgaag gtctttcaat aacatatcaa aagcagacta atgcttctga tgcatatgtt
      241 tcaaatacat cttcaataga aaaccttgtt tcaactccgg ctcttttgac tatgatcgtt
      301 gaagcttctt gcaaaatgct tgatagttta attccacagc aatgtttaac tgttggaaac
      361 cgattagaaa tttctcatat aaatccaaca ttggtcggtg atacaataac attgatactt
      421 acggtagaaa aagtagatgg caataagata cttcttgata tcgtaggtgc ggattcgaac
      481 ggaaggattt gcttaggaaa atatcataaa gttattgtgg agagaaacaa gttgatggaa
      541 aatgcctata aaaggtcgaa agattggcag tgatacaata aaatgtgcaa gatagattaa
      601 ataatactat caatatttat agaaaggatt agataaatgg ctaataaata tcatgatttt
      661 tctccaaaag aagtaagaca attaataaga gaagaaaaaa ttcaagaacc gacatccggt
      721 atgtgcggtg gatatgctca agcaaatctg gtaatattac ctaaaaaata cgcagatgat
      781 tttaaattat ttgcggagta taatcctaaa ccatgtccta tacttgaaat aaccgaaccg
      841 ggaagttata ctacaacaaa gactgcagat aaggcaaaca tcattaccga tattccaaaa
      901 tattttatat ataaaaacgg agtaagagta gatgaagtgt tatttgcaga aaaatattgg
      961 ggagacgatt tcgtttgttt ccttatcggc tgcagtttct catttgaaga agcaatgata
     1021 agagaaggaa ttgaaatcag acatatttca atgggtcgta atgttcctat gtataaaaca
     1081 aatatagagg ctaaaccagc cggaatattc aaaggaccat atgttgtaag catgagacct
     1141 atgaaacccg aggatgtaga taaagcatat gaaataacag ggaaattccc gcatgttcat
     1201 ggtgctccga tacatcacgg cgaccctgcg gaaataggaa ttaaggatat caataaacct
     1261 gattatggcg aagcagttat aataaaagaa ggagaagtac cggtattttg ggcatgtgga
     1321 gttacaccgc aagctgcaat tgaaaatgct aaacctgaaa tagttataac tcatgcaccg
     1381 ggtcatatgt ttataaccga tattcttaat gaagatatcg aaagcatatt ctagaggtca
     1441 aacaattaat tattcgaata tgaaacccgg ctttaataag tcgtgataat atatgtcaat
     1501 tgcctcaagg atagcaggac aatacaaata aatccaaaat atttaaacca aatattttgg
     1561 attttatttg ttagcattct taactcaggg tttatgccgg tttttattat tgcaaatcac
     1621 caattactgt actatactat aatgaataca atcaatgaag caaagacttt ggtctttgca
     1681 taggcttgac aaaccttatg tttgtcatcc tgagcacagc gaagaattgg agtatatatg
     1741 aaagacattg aattagcaaa acaaatattg aataatgaaa ataaaacaat agtaattgtc
     1801 aaggatggaa aagtaatttt ttcaagtgaa aataaaggaa ttaaacctgt ttgtgaagct
     1861 tttaatgagc tcaaagacga acttaaaggc tctagtgtgt ctgataaagt tgttggtaag
     1921 gcggctgcaa tgatttataa acatgctgat atcaaagaac taagcactaa attaatttct
     1981 caaaatgctg tggatatttt gaaaaacact tctattgtgt ttgaatatca aaaattggtt
     2041 tcatacataa aaaatcgtga acaatcagga atgtgtccta ttgaaacact gtcgcttgat
     2101 gttaataata ttgatgagct gctttcaaaa atatcagact ttttaggaaa agaaagaaca
     2161 ttggtgctta taaaaccgga tgcaattgaa agaaaactta tgggtgaaat aatttctgtt
     2221 tatgaaaaga attttaacat aattgatatg aaaatgatta agccatctgt tgaaattgca
     2281 gaagaacatt attatgagca cagggacaaa ccatttttca atgaacttat ttcttatatt
     2341 atcaggggaa atgtatgtgc attgattatt gaaggtaaaa atgtaataga aagagtaaga
     2401 aaaattaacg gagcaacaga cccaaaaaat gctgagccgg attcaatcag gggaaaattt
     2461 gctttatcaa aacaggaaaa ttcagtacat ggctcagatt ctaaggaaag tgcagaaaga
     2521 gaaataaaga tttggttcgg ataagaaaag agggacatct tgaaggtaat tcttcggata
     2581 gaatagaaga acgtcctgta tgggaggaaa taatgaataa aaagtttgaa ggtactaagc
     2641 caataataga cgaaaaaaca tacattgccg atggtgctca aatcataggc aaggtaacga
     2701 taaaagaata ttcaagtatt tggtttaata cggttgtaag aggagatgta aactatatag
     2761 aaataggcag gtatacaaat attcaagata acagcgttgt acatgttgcc gacgattatc
     2821 cggcaataat tggagattac gtaacaatcg ggcacaatgc aactgttcat gcctgtaaaa
     2881 ttgaagacca ctgcttaatc ggtatgggtg caattattat ggacggtgct gttataggaa
     2941 gaggaagcat tatagctgca ggtgcggtag ttaccaacaa ccaaaaaata cctccttttt
     3001 ctgttgccgc aggaatacct gccaaagtaa ttaagactgt acctgaagaa gatataatta
     3061 aaatacattc ccaagcatta aaatataaac atgtttggtc aatcaggtat ggtattatgc
     3121 ctgattgcgg cggagagtca tatgatggcg gggaaattgt ttagttacat attaaattta
     3181 tggagcaatt atggtaatca agaaagattt ttattatgat tcagccgata aaaaatcaaa
     3241 aattcatgct gtaaaatggg tgcctgatgc tgaaataagg ggtgtacttc aaattgctca
     3301 cgggatgctc gagcatattg acaggtacga ctgctttgca cgctatatgg ccgaaaacgg
     3361 aatactgact gcaggaaatg accatcttgg acatggagat tcagtgcttg ttgaagaaga
     3421 ccgcggtttt ttcggtgaag gcgacggaaa taagttagta accaaagata tgcgaaaatt
     3481 aagagaaata ttgaaggaag aatatgaagg cttgccctat tttatattag gccacagtat
     3541 gggttcattt ctggtaagac agtatatttc aatatataat gatttgaatg gtgccttact
     3601 cgtaggaacc gcttatcagc catatttatc agtaaaagca ggtctgataa tttctaagtt
     3661 aatggcagtt tttaaaggat ggagatacag aagcaagttt ataaataagc tgacaatcgg
     3721 aggcaacaat aaatattttg agcctgctaa aaccaagtcg gattggctta gcagggacga
     3781 agaagcagta gatgcttatg ttaaggatag aaaaatagat tttatattta ccttgaatgc
     3841 tttttattac atgtttaaag gaatgctgta tttatatagt aaagaatata tataaatttg
     3901 gctgttgaaa gaatagaatt ttattcctgt caacctgaaa gagaccgttg aaaaaagcag
     3961 tattttcgac aggagcacca tcggttccat tttgaatctg atcagagctt catttttggc
     4021 ctttcagctt cggtttacca ggtcttttag ctcctttttc acctcttttc ctccaacatc
     4081 gacaatcctc ccttgttcag cactaacatg ccccataaac cgggcagttg aagcttactc
     4141 cgctgatcag cttcaccatc cgtatcaaat tgagtgcgat cgcggtaaga tatgcctgaa
     4201 ccccatactt ctccaatcca atataccggc a
//
LOCUS       DAOSOW010000129         4226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_21029_length_4226_cov_4.508271, whole genome shotgun sequence.
ACCESSION   DAOSOW010000129 DAOSOW010000000
VERSION     DAOSOW010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21029_length_4226_cov_4.508271"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLD67_04415"
     CDS             complement(<1..367)
                     /locus_tag="PLD67_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT21796.1"
                     /translation="MNIFEMQYQNSIKKEGPLAERMRPLNLSEFVGQEHIVGEGKILN
                     RAIEADKLTSAIFYGPPGTGKTTLAKIIANTTNNNFTTLNAVTSGIKDIKEKISYAVD
                     ELSIYNKRTILFIDEIHRFN"
     gene            complement(464..886)
                     /locus_tag="PLD67_04420"
     CDS             complement(464..886)
                     /locus_tag="PLD67_04420"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOT21797.1"
                     /translation="MDNIGKIEKIDGNMAVISVKRVSSCGDSCKSCSSACKVNSVIIE
                     TDISDVKDEINVGDYVEIRAENEVMLKHIAVLYGLPLLLMLFTVFIFQMILKGPNKDI
                     ISAVASLFSLIISFYLLKAYDKSEMKKNVLKFTVGKKL"
     gene            complement(909..2681)
                     /gene="aspS"
                     /locus_tag="PLD67_04425"
     CDS             complement(909..2681)
                     /gene="aspS"
                     /locus_tag="PLD67_04425"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009372686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOT21798.1"
                     /translation="METMGTLRRTNMCGELNKEHIGKEVVLMGWVQKRRSLGGLIFAD
                     LRDITGLIQIVFDADVNKEAFEKADSLRSEYVIAIKGKVFERQSKNLEMATGEIEVYA
                     QELRILDTAETPPIYIKDNDDVSEQMRLKYRYLDLRKPSMQKNLLMRSKTSKVIRDFL
                     YENGFNEIETPFLAKPTPEGARDYLVPSRISPGNFYALPQSPQIYKQLLMVSGMNRYF
                     QIVRCFRDEDLRANRQPEFTQVDIEMSFVDAEDVMAINERLIQRIFKEVKNIDIKLPL
                     RRMSYNEAMDKYGCDKPDLRFGYELKNITYVFKNSEFNAFKSVYENGGLIKCIKIDGA
                     SDDYSKKGISKLEKFVKSHGAKGLAWFKYENGLFDSPIAKFLSEGEINELISLLELKE
                     KDIVFIVADNESAVNTALSALRIEIAEERNLIDNNVYELVWITDFPLFEYSEEEERYV
                     AKHHPFTSPVDEDIDKLESAPEEVRAKAYDIVINGDEIGGGSIRITNRDLQNRMFKAL
                     GFTEEEAQKKFGYLLEAFKYGVPPHGGIAYGLDRFMMLLTGANSIKDVIAFPKTQSAS
                     CLMTESPSQVSEKQLDELKIEINE"
     gene            complement(3034..3798)
                     /gene="pdaA"
                     /locus_tag="PLD67_04430"
     CDS             complement(3034..3798)
                     /gene="pdaA"
                     /locus_tag="PLD67_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HOT21799.1"
                     /translation="MIVVLLFACLLIYNTFKNFSVTVSTNISNWGLKFNENGTAPMGN
                     AGKDFLKKYNAYYVGEPDKKYIYLTFDAGFENGNTPNILDTLKKHDVKAAFFLVGHYL
                     EKEPGLVKRMVREGHIVGNHTYHHPDMSLISDKESFRRELTSLEELYKEITGEEMPKY
                     YRPPQGKFSEENLRMADELGYKTIFWSLAYVDWYKDDQPTKEQAFSKLIPRIHPGAVV
                     LLHSTSDTNEMILDELLTKWKDLGYTFKTLDDLTAN"
     gene            4024..>4226
                     /locus_tag="PLD67_04435"
     CDS             4024..>4226
                     /locus_tag="PLD67_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21800.1"
                     /translation="MEHFMETAVHYVAYTLEIVSIIIVAYSAVTTLIKYLMSKFEFTN
                     KQAKIEFSRALEMALAYMLGAEIL"
BASE COUNT         1215 a          884 c          621 g         1506 t
ORIGIN      
        1 tattgaaacg atgaatttca tctatgaaaa gaattgttct cttattataa attgaaagct
       61 catcaactgc ataacttatt ttttccttta tatccttaat accgcttgta acggcgttaa
      121 gagtcgtaaa attattgttt gtggtatttg caataatttt agccaaggtt gtcttgccgg
      181 ttcccggagg tccgtaaaaa atcgcagagg taagcttgtc agcttcaatt gctctgttta
      241 aaattttgcc ttcgcccaca atatgctctt gcccgacaaa ttcacttaaa tttaaaggtc
      301 tcatccgctc tgcgagagga ccttcttttt ttatggaatt ttggtattgc atttcaaata
      361 tattcattgt atcaatcctt atgattcttc aatgcgttca gaatgacgcc tcacgggatt
      421 catcactgcg cccagatgac ctttgtcatc ctgatcgtat gcattaaagt tttttaccaa
      481 cagtaaattt taatacattc tttttcattt cactcttatc gtaagccttt aagaggtaaa
      541 aagaaattat taacgaaaac aatgaagcta ctgcagagat tatatcctta ttgggtccct
      601 tcaatatcat ttgaaaaata aaaaccgtga ataacatcaa taacaaagga agtccgtaaa
      661 gaaccgctat atgcttaagc atcacttcat tttccgctcg aatttccaca taatctccga
      721 catttatttc atcttttaca tccgaaatat cggtttctat aataacgctg tttactttgc
      781 atgcggaaga gcagcttttg cagctgtccc cgcaggatga taccctttta acagaaataa
      841 cagccatgtt accgtcaatt ttttcaatct ttcctatatt gtccatttga tcttccttcc
      901 tataaaactt actcattaat ttcaattttc agttcatcaa gctgcttctc agatacctgg
      961 ctgggagatt ctgtcatcag gcaggaagca ctttgtgttt ttggaaatgc aattacatct
     1021 tttatactgt ttgcgcctgt caaaagcatc ataaagcggt ccaatccata tgcaattccg
     1081 ccgtgaggag gcacgccgta tttaaatgct tcaagcagat aaccgaattt tttttgtgct
     1141 tcttcttccg taaatcctaa tgctttaaac attctgttct gcaaatccct gtttgttata
     1201 cgtatgcttc cgccgccaat ctcgtcaccg tttattacga tgtcatatgc ttttgccctc
     1261 acttcttccg gagctgattc taatttatct atatcctcat ctacaggtga tgtaaatgga
     1321 tggtgttttg ccacgtatct ttcttcttct tcgctgtatt caaacagtgg aaaatcagta
     1381 atccacacta attcataaac attattgtca attaaatttc tttcctcggc tatttcgatt
     1441 ctcaaggcgc tgagggcagt atttacggca gattcattat cggcaactat aaaaactata
     1501 tctttttcct ttagctccaa caaagaaatt agttcattta tttcgccttc acttaaaaat
     1561 tttgcaatcg gagaatcaaa taggccgttt tcatatttaa accaggctaa tccttttgca
     1621 ccgtgtgatt ttacaaattt ctctaactta gaaatgcctt tctttgaata atcatccgat
     1681 gctccgtcaa tttttatgca tttaatcaag ccgccgtttt catagactga tttgaatgca
     1741 ttaaattcag aatttttaaa cacataagta atatttttca gctcatatcc aaaacgtaaa
     1801 tcaggtttgt cgcagccgta tttatccata gcttcattat aagacatacg tcttaatgga
     1861 agcttaatat cgatattttt tacttccttg aatattcttt gaataagcct ctcatttatc
     1921 gccattacat cttctgcatc cacaaaggac atttcaatgt ctacctgtgt gaattcaggt
     1981 tgacggtttg ctcttaagtc ttcgtctctg aagcatctta caatctggaa atatctgttc
     2041 attcccgaaa ccatcaaaag ctgcttataa atttgcggcg attgaggcaa tgcatagaaa
     2101 ttaccgggtg aaattctgct tggtaccaga tagtcccttg ctccttcagg tgtaggtttt
     2161 gctaaaaatg gtgtttcgat ttcattgaac ccgttttcat ataaaaagtc ccttataacc
     2221 tttgatgtct tacttctcat caatagattt ttttgcattg aaggctttct caaatcaaga
     2281 tatctgtatt ttaacctcat ctgctcggaa acatcatcat tatcctttat ataaatggga
     2341 ggagtttcag cagtatctaa tattcttagc tcctgtgcgt aaacttctat ctctcccgta
     2401 gccatttcaa ggtttttaga ctgtctttca aatacttttc ctttaattgc tattacatat
     2461 tcacttctta aactatctgc tttttcaaaa gcttccttat ttacatcagc atcaaaaaca
     2521 atttgaatta atccggtaat atcccttaag tcggcaaaaa taagtccgcc taagctcctt
     2581 cttttttgta cccatcccat cagcacgact tccttgccaa tgtgttcctt attcaactct
     2641 ccgcacatat ttgttcttct aagtgttccc atcgtttcca tagtatcctc cattgtttaa
     2701 gtagggacat tcctctgacc ggcaaaggca atctttattg aaagaggtgt gtcccaatac
     2761 ctataataat aaaaacccgt cccctgctta gggacgagta taacccgcgt tgccacccta
     2821 tttggactaa taagtcccac tcattagaat ttgcattttt gaggtgtctt catcaaaagt
     2881 tgataacagg ctcccatcgt ccctgtcttg cttaaatcat tgctttatga ctactcttcc
     2941 cctgcaatta tttacaacta aattaccaca ccccatcttg tttgtcaaca ataaaattaa
     3001 attttgatga cagtaccccc gtccccttgt catttagttt gctgttaaat catccaatgt
     3061 tttaaatgta tatcctaaat ctttccactt tgttaacagc tcatccaata tcatttcgtt
     3121 tgtatcggag gtagagtgca ataagactac tgctccggga tgtattcggg gaattaattt
     3181 gctgaatgcc tgctccttgg taggctggtc atccttatac cagtcaacat acgcaaggct
     3241 ccaaaaaatc gttttatatc ccaattcatc agccattctt aaattttctt cactgaattt
     3301 gccttgagga ggtctgtaat actttggcat ctcttcaccg gttatttcct tgtataattc
     3361 ttctaaggat gttaactccc tcctaaatga ttccttgtca gaaatcaatg acatatcggg
     3421 atgatggtag gtatgattac ctactatatg accctctcta accatccttt tgacaagtcc
     3481 gggctctttt tccaaatagt gtcccactaa gaaaaatgca gccttcacat catgtttttt
     3541 taatgtatca agtatattgg gagtatttcc gttttcaaac ccggcatcaa atgtgagata
     3601 aatatacttt ttatccggct cccctacata ataggcatta tatttcttta aaaaatcttt
     3661 tcctgcatta cccattggtg ctgttccgtt ttcattgaat tttagccccc aattcgatat
     3721 atttgttgaa actgtcaccg aaaaattctt aaaagtattg taaatcaata aacatgcaaa
     3781 caataaaact actatcatta taataataaa tacctttttg tttattctta ataccatata
     3841 tctcacccct ttatttattt atataaataa acaggcttaa tatgactggc tataaattat
     3901 ttattattct tttttataat aggatgcata tttccaaaat cactctaaaa gggctttgac
     3961 attattaata aaatgcaata taatagcata cgttaaatat aatataaatt tggaggtttt
     4021 taattggaac acttcatgga aaccgcagtt cattatgtcg cttacacctt agaaattgta
     4081 agcataataa ttgttgctta cagtgcagta acaacattaa taaaatatct aatgagcaaa
     4141 ttcgaattta ctaataaaca agctaaaata gaattttcaa gggcgttgga aatggctttg
     4201 gcatatatgc tgggcgcgga aattct
//
LOCUS       DAOSOW010000130         4222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_21058_length_4222_cov_3.567315, whole genome shotgun sequence.
ACCESSION   DAOSOW010000130 DAOSOW010000000
VERSION     DAOSOW010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21058_length_4222_cov_3.567315"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLD67_04440"
     CDS             complement(<1..375)
                     /locus_tag="PLD67_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21801.1"
                     /translation="MKENLTELVFILDRSGSMSGLETDTIGGFNSMLEKQKKESGEAF
                     VTTVLFDDKYELLHDRYDIKGVNLLTEKEYYVRGSTALLDAIGITINNIGKALSDTNE
                     EDRPGKVLFVIITDGMENSSREF"
     gene            complement(450..1448)
                     /locus_tag="PLD67_04445"
     CDS             complement(450..1448)
                     /locus_tag="PLD67_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HOT21802.1"
                     /translation="MPLQIIRNDITKMDVDVIVNAANSALKMGGGVCGAIFRAAGEKE
                     LQAECDSIGGCETGNAVITKGYNLPAKYIIHTVGPVWNGGKNKEEKLLRSCYIKSLNL
                     AKEHKLESIAFPLISSGIYGYPKAEAFKAAVTSIQDFLLENEMMVYLIVFDKAAVEIS
                     EKLYTSIEKYIDDKYVDEKAEIRENRLQVEDAAIKYMVSAPTLIKTTKDKKSLEDIVS
                     QLDETFSEMLLRLIDEKEMTDVETYKKANIDRKLFSKIRTDKFYRPRKTTALALCIAL
                     ELNLDQTKDLLARAGYALSPSSKFDLIVEYFIEQGIYNIFEINEALFAFEENLLGV"
     gene            complement(1495..3219)
                     /locus_tag="PLD67_04450"
     CDS             complement(1495..3219)
                     /locus_tag="PLD67_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOT21803.1"
                     /translation="MKTCTLTINNKKITVEAGTTLLEAAKSAGYFIPTLCNHPDQEIK
                     ANCRICVVEDKSAGKFVASCSTKVYDGAEIHTNTKEVRELQRGILELILANHPNDCLN
                     CIRNESCELRKLCERFHVRNYNLNHETDIYPIDNTSGVLVRDRNKCIKCMRCVEACQE
                     TQMVGILSPSYRSVNTNISAGFGENYDETLCTFCGQCSAVCPVGAIYEYDETEKVWEA
                     LHDPKKHVIVQTAPAVRVGLGEEFGMPRGSKVTGKMVSALKMLGFDKVFDTDFAADLT
                     IMEEGYELLSRIKNGGTLPMITSCSPGWINYIEGFYPELLDHLSTCKSPQQMFGAVAK
                     TYYAKKAGIDPADIFTVSVMPCTAKKFECKRPEMNSSGYRDVDVALTTRELARLIKNA
                     GIDFTNLKNEDFDSIMGSSTGAAVIFGATGGVMEAALRTVYEVVTGKTLEKIEFDEVR
                     GLEGIKEATIDLDGTPVKVAVAHGLANANKVMEMIKNGEADYTFIEIMSCTGGCAGGG
                     GQPVGANKSIKARRIDTIYNIDKEMPIRKSHDNPEVQQLYEEFLGEPNGHLSHKLLHT
                     HYHQRRKK"
     gene            complement(3228..>4222)
                     /locus_tag="PLD67_04455"
     CDS             complement(3228..>4222)
                     /locus_tag="PLD67_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT21804.1"
                     /translation="NADEGEPGTFKDRYFLEYDPYRIIEGMTIAAYAVGGREGYIYIR
                     EEYGRQKEIFLKAIEAGKAAGFLGDNILGTDLSFDIKVVSGAGAFICGETTALIQSIE
                     GGSGRPRIKFQRTAVSGLFNRPTLANNVESYCVVTTLFNNDAQEYLSHGTEKSKGTKV
                     ISLCGNVENPGVYEVPFGITLREIIYDIGGGIKDGRDFKFAQIGGASGPLIPKSMLDI
                     PYSYEDFGKEGYSLGSGAVLVADDTNSVADFMVTVQEFFVHESCGKCTPCREGNRQLL
                     KLAHKIADKKVTVEDFLTVKRIANVMDKCSFCGLGTAAPTAMVTAIQHFYDMYN"
BASE COUNT         1149 a          954 c          653 g         1466 t
ORIGIN      
        1 aaattctctg cttgaatttt ccatgccgtc ggttataata acaaacaata ccttaccggg
       61 tctatcttct tcatttgtat cggacaaagc tttgccgata ttattgattg taattcctat
      121 tgcatcgagc agcgcggtag aacctcgcac ataatattcc ttttccgtaa gcaagttaac
      181 gccttttata tcatatctgt catgaagtaa ttcgtatttg tcatcaaaca ataccgtggt
      241 gacaaatgct tctccgcttt cctttttctg cttttcgagc atagaattaa atccgcctat
      301 tgtatccgtt tcaagaccgg acattgagcc gcttctgtcc aatatgaata ctaattccgt
      361 taaattttct ttcatttcga tacctccaat catttattga ttgaaggata tcacttttat
      421 gaatataagt ggtcgcttta aatgcgacat tatactccta acaaattttc ttcaaatgca
      481 aataaagctt catttatttc aaaaatatta taaataccct gctcaataaa atattctact
      541 attaagtcaa atttgctgct gggagaaaga gcataaccgg ctcttgcaag caagtcctta
      601 gtttggtcta aatttaattc caaagcaatg caaagagcaa gagctgttgt ctttctcggt
      661 ctgtaaaatt tatctgtcct tattttagaa aaaagtttcc tgtcaatatt agctttttta
      721 taggtttcaa catcagtcat ttccttttcg tctattaatc ttaaaagcat ttcagaaaag
      781 gtttcgtcta actgagatac aatatcttct aaactttttt tatcctttgt agtctttatc
      841 aatgtcggtg cagacaccat atattttatg gcagcatctt caacctgaag tctgttttct
      901 ctgatttcgg ctttttcatc aacatattta tcgtcaatat atttttcaat cgaagtgtac
      961 agcttttcac ttatttcaac tgcagcttta tcgaagacaa tcaaataaac catcatttca
     1021 ttttccaata aaaaatcctg tattgaagtt acagctgctt tgaatgcttc cgccttggga
     1081 taaccgtaaa ttcccgagga tattaaagga aatgctatgg attctaattt atgttcctta
     1141 gctaaattaa gagattttat ataacaactc ctcaatagtt tttcttcttt atttttcccg
     1201 ccgttccata caggacctac agtatgtata atatactttg cagggagatt gtaccctttt
     1261 gttataactg cattacctgt ttcacaacct ccgattgagt cgcattccgc ttgaagttcc
     1321 ttttctcctg ccgccctgaa aatagcaccg caaacaccgc cgcccatttt aagagcagaa
     1381 tttgcagcat taacaataac atccacatcc attttagtta tgtcatttct tattatctgt
     1441 aagggcaccg tctcacctcc cttcatacta tattgttaaa tcaaaatata taacttattt
     1501 cttacgtctt tgatgatagt gagtatgaag cagtttatga gacaaatgac cgtttggctc
     1561 tcctaaaaac tcttcataca gttgttgtac ttcaggatta tcatgagatt ttcttatggg
     1621 catttctttg tcaatattat aaatagtatc aattcttctt gccttaattg atttatttgc
     1681 tcccaccggt tgaccgccgc cgcctgcgca tcctcccgta cagctcatta tttcgataaa
     1741 tgtataatcc gcttcaccat ttttaatcat ttccataact ttattggcat ttgccaagcc
     1801 atgggctaca gcaactttaa caggtgtgcc atccaaatct atggtagctt cctttattcc
     1861 ttcaaggcct cttacttcgt caaactcgat tttttccaac gtttttccgg taacaacctc
     1921 atacaccgtt cttaaagcag cttccataac accgcccgtt gcaccgaata taacggctgc
     1981 cccggtagat gaacccatta ttgaatcaaa atcttcattt ttcaggttag taaaatctat
     2041 tcctgcgttt ttaatcaacc tggcaagctc cctggttgta agcgcaacat caacatccct
     2101 ataccctgaa gaattcattt caggtctctt gcactcaaat tttttggctg tgcagggcat
     2161 tacagatacg gtaaatatat ccgcgggatc tattcctgcc tttttggcat aatatgtttt
     2221 tgccacggct ccaaacattt gctgcggaga cttgcaggtt gaaagatggt ctaacagttc
     2281 aggataaaaa ccttctatat aatttatcca ccccggactg caggatgtaa tcattggtag
     2341 ggtaccgccg tttttaatcc tcgacaaaag ctcatatcct tcttccatta ttgttaagtc
     2401 tgcagcaaaa tcagtatcaa aaaccttgtc aaatcccaac attttaagtg ccgataccat
     2461 tttaccagta acttttgagc ctctcggcat tccaaattct tcacctaagc cgactcttac
     2521 cgctggagca gtttgaacga taacatgttt tttcggatca tgcagggctt cccaaacctt
     2581 ttctgtttca tcatactcat aaatagcacc gacggggcat actgccgagc attgtccgca
     2641 gaatgtacaa agtgtttcat cataattttc accaaaacct gcactgatat ttgtattaac
     2701 agacctgtag gaagggctta atattccaac catttgtgtt tcttggcatg cttcgacaca
     2761 tctcatgcat tttatgcatt tatttctatc ccttaccaat actcctgaag tattatctat
     2821 ggggtatata tcggtttcgt gattcaaatt gtagtttctt acatggaacc tttcgcaaag
     2881 ctttctaagt tcacaagatt cattgcgtat gcagtttaaa caatcattgg gatgattggc
     2941 taatatcagc tccaaaatac ctctttgcag ctctcttact tcttttgtat tcgtgtgaat
     3001 ttcagctccg tcataaacct ttgtagaaca tgaagcaaca aatttaccgg cacttttgtc
     3061 ctctaccaca caaattctgc agtttgcttt tatttcttgg tcaggatgat tgcatagggt
     3121 cggtataaag taacctgctg attttgctgc ttctaataag gtagttcctg cctctacagt
     3181 tattttctta ttatttatag tcaatgtaca agttttcatg tctcctccta attatacata
     3241 tcataaaagt gttgtattgc cgttaccatt gctgtaggtg cagcagtacc taatccgcag
     3301 aatgagcatt tatccataac gttagcaatg cgtttaacgg ttaggaaatc ttccactgtt
     3361 acttttttat ccgcaatttt atgtgccaat ttcaaaagct gcctgtttcc ctcacggcaa
     3421 ggtgtgcatt tgccgcagct ttcatgaaca aagaattcct gcacggtgac cataaaatct
     3481 gcaacgctgt ttgtatcatc ggccaccaat acagctccgg agcccagact ataaccttcc
     3541 tttccaaaat cttcataaga gtatggaatg tctaacatgg attttggaat aaggggacct
     3601 gaagccccgc ctatttgagc aaatttaaaa tctcttccgt cttttattcc gccgcctata
     3661 tcatatatta tttctcttaa tgtaataccg aatggaacct cataaactcc cgggttttca
     3721 acatttccgc acaagcttat aacctttgtt cccttgcttt tttcggtacc gtgacttaag
     3781 tattcctgtg catcattatt aaacagggtt gtaacaacgc aatatgactc aacattattg
     3841 gcaagcgtag gtctgttaaa taaacctgat acggcagtcc tttgaaattt aatccttggc
     3901 cttccgctgc cgccttcaat ggattgtata agagctgttg tttcgccgca aatgaatgct
     3961 cccgcacctg ataccacttt tatatcaaag gataaatcag tccctaatat attatctccc
     4021 aaaaatcctg cagccttgcc agcctctatt gcttttaaaa atatttcctt ttgcctgccg
     4081 tattcctctc tgatatatat ataaccttct ctgcctccga ctgcatatgc agcaattgtc
     4141 attccttcaa ttattctgta aggatcatat tccaaaaaat atctgtcttt aaatgttccc
     4201 ggttctcctt catcggcatt gc
//
LOCUS       DAOSOW010000131         4221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_21067_length_4221_cov_3.432549, whole genome shotgun sequence.
ACCESSION   DAOSOW010000131 DAOSOW010000000
VERSION     DAOSOW010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21067_length_4221_cov_3.432549"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..487
                     /locus_tag="PLD67_04460"
     CDS             53..487
                     /locus_tag="PLD67_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21805.1"
                     /translation="MLFESDIDIDYNLAYTAVVSADEEDKKDICILMVPEQKAHNMTS
                     NMSALRLANLCIDEPEVVMSASDGTILFSDIKYGKVSCNVAIPSGRYTLQLKKPDGTE
                     ILDHWIDAAPYMHYTLFIVGMKNDGSIKIIVPEDGVNYLDLC"
     gene            599..1294
                     /locus_tag="PLD67_04465"
     CDS             599..1294
                     /locus_tag="PLD67_04465"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HOT21806.1"
                     /translation="MKIIGIIGGMGPLATVHLFERIVLRTKAEKDQDHIRILIDNNTK
                     IPDRTLAILGNEESPVNELVNSAKMLENSGADFLIMPCNTAHYFIEEIKKNISIPFIN
                     MPEEAVKHTYDKYAKDAVVGIMATDGTIKTKIYEKYYAELGIKTVVPIKTQEKIMEFI
                     YDVIKKGNYDAGTSLFFECEDELKELGANVFLLGCTELSSAQYIYKLEGNFINPMEIL
                     AETSIIFAGGQLN"
     gene            1354..2175
                     /gene="purR"
                     /locus_tag="PLD67_04470"
     CDS             1354..2175
                     /gene="purR"
                     /locus_tag="PLD67_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HOT21807.1"
                     /translation="MKKYKRNQRIGALMKIFAEKPNCLFSYNYFCEIFNAAKSTISED
                     VAIVKELAAEMNFGRIETISGVSGGVMFIPILGKKELNEILEQLCSLFSQKNRILPGG
                     FIYMTDIFNNPRIVSNIAEVISSQFSNKHIDYIVTVETKGIPVAIMTGHKLNVPVAVI
                     RRNNKITEGATVSINYVSGSSNNIQTMSLSKRSLKENAEILIVDDFMKGGGTCKGMED
                     MMREFNANVVGTAVVIETIEPEKKLVDNYTSLLILNNKSIKEGVIEIKPNPKLLK"
     gene            2273..2566
                     /gene="spoVG"
                     /locus_tag="PLD67_04475"
     CDS             2273..2566
                     /gene="spoVG"
                     /locus_tag="PLD67_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HOT21808.1"
                     /translation="MKISDVRVRKINSEGKMKAIVSVTFDDCFVVHDIKIIEGQEGLF
                     IAMPSRKMPDGEFKDIAHPINSETRNLVAEAVFKAYEEKRTEESENVEEAALM"
     gene            2777..4147
                     /gene="glmU"
                     /locus_tag="PLD67_04480"
     CDS             2777..4147
                     /gene="glmU"
                     /locus_tag="PLD67_04480"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOT21809.1"
                     /translation="MSMSIILAAGEGTRMKSDLPKTLHKVCGKEMIKYIIEASLNSGI
                     EKNIVVLGHGKEKVGHNIKDMNVQTVEQPIGEGHPYGTGYAVMAAENHIDDGETVLIL
                     CADGPLVKKETLIDFMNYHFESNCAASIMTAYLDNPRGYGRIVRKSDMSIDEIVEEKD
                     ASEEIKNIKEINTGIYCFKGELLKYALKKITKNNSQNEYYLTDTIKVLNNEGANIGGW
                     LLEDSDDIKAVNDRVQLAEVTKIMQKRINQRHMYSGVTIIDPDSTYIDDSVKIGRDTV
                     IYPNTYLEGGTVIGSNCTIGPNSRIIDSTVRNNVTVESSKVIESYIDDGTNVGPFAYL
                     RPGSNLGKNVKIGDFVEVKKSIIGDNSKSSHLAYIGDAEVGKNVNIGCGVVFVNYDGK
                     KKHKTIVGDGAFVGSNSNLVAPVTIEKGGYVAAGSTITEDVKEGDLAIARARQVNKEG
                     RGKNRF"
BASE COUNT         1555 a          567 c          890 g         1209 t
ORIGIN      
        1 attcgacaat gttaagtttt tgttatctaa ataacactct ccaaatggta atttgttatt
       61 tgaatcggat atagacatag attataactt ggcatacaca gcagtcgtct cagctgatga
      121 agaagataag aaggatattt gcattttaat ggtacctgag caaaaagcac ataatatgac
      181 aagtaatatg tcagcattgc ggttggcaaa tttgtgtatc gatgaaccag aagtagtaat
      241 gtcggcgtcg gacggaacta ttctattttc tgatattaag tatggaaaag tatcgtgtaa
      301 tgtggcaatc ccatcgggaa gatatacatt gcaattaaag aaacccgacg gcactgaaat
      361 cttagatcat tggatagatg ctgcacctta catgcattat accttgttta ttgtagggat
      421 gaaaaatgat ggcagcataa aaattattgt acctgaagac ggagtaaatt atttggattt
      481 atgttaatta atgacaaggg gacgggggtg ttgtcatctt tgatgacaac acccccgtcc
      541 ccttgtcaca atgtaagtaa tatgatataa tctagcaata aaaattatga ggtggactat
      601 gaaaataata ggaattatag gcggtatggg gcctcttgcc actgtacact tatttgaaag
      661 aattgtttta agaacaaagg cagaaaaaga ccaagaccac attcgtatct taattgataa
      721 caatacaaaa attccggaca gaactttagc catactcggt aatgaagaaa gtccggtaaa
      781 tgaattggtt aattcagcga agatgcttga aaacagcggt gcggactttt taataatgcc
      841 ctgcaatact gcacattact ttatagagga aataaagaaa aatatcagca ttccctttat
      901 taacatgcct gaagaggcag ttaagcatac atatgacaaa tatgcaaagg atgctgtagt
      961 tggaataatg gctactgatg gaactattaa aacaaaaatt tatgaaaaat attatgccga
     1021 gttggggata aaaacagttg taccgataaa aacacaggaa aaaataatgg aattcattta
     1081 tgatgttata aaaaaaggca attacgatgc aggtacaagt cttttctttg aatgcgaaga
     1141 tgaattaaag gaattgggag caaacgtatt tttgttaggc tgcactgaac tttcgtcagc
     1201 acaatatata tataaattgg aaggaaactt tattaacccc atggagattt tggctgaaac
     1261 ctcaataata tttgcaggag ggcagctgaa ttaagtcttg atttatacga acatttgtaa
     1321 tataattata taaaaacatt cgctggtgat aaaatgaaaa aatacaaaag aaatcaaaga
     1381 atcggtgcat tgatgaaaat ttttgccgaa aaaccaaatt gtttgttttc atataattat
     1441 ttttgtgaaa ttttcaatgc agcaaaatca acaataagtg aagatgtggc aatagtcaag
     1501 gaattggctg cagagatgaa ttttggcagg attgaaacca tttcaggagt ttcaggcgga
     1561 gttatgttta tacctatatt gggtaagaag gaattaaatg aaatacttga gcagttatgc
     1621 tcattatttt cgcaaaaaaa ccgaatactg cccggcggtt ttatttatat gaccgatatt
     1681 ttcaacaacc ctcgaattgt gtcgaatatt gccgaagtaa tttcttcaca atttagtaat
     1741 aaacatattg attatatagt taccgtagag acgaaaggta tacccgtagc tattatgaca
     1801 ggccataagc tgaatgttcc tgtggccgta atcaggagaa acaacaaaat tacagaaggg
     1861 gcaacagtca gcataaacta tgtttcgggt tcatcaaaca atattcaaac catgtcatta
     1921 tcaaagaggt ctttaaaaga aaatgctgaa attctaatag ttgacgactt tatgaaaggc
     1981 ggcggaacct gcaagggaat ggaggatatg atgagggaat ttaatgcaaa tgttgtaggg
     2041 actgctgttg ttattgaaac tatagagccg gaaaagaaat tagtagataa ttatacttca
     2101 ttattgatac ttaataataa aagtataaaa gaaggtgtta tcgaaataaa accaaatccg
     2161 aaattgttaa aataatttca tatatttatg aaaatgtttt tttgctattt ttgggtattt
     2221 atagtataat gaataaaatt ggacataatt attgggggaa ggtggtgaca gtatgaaaat
     2281 atcagacgta agggtaagaa aaattaattc tgagggcaag atgaaggcaa tcgtttccgt
     2341 aacctttgat gattgtttcg tggtacatga tattaaaata attgaaggtc aagaagggtt
     2401 gtttatagca atgcccagta ggaagatgcc ggacggagag tttaaagata tagctcatcc
     2461 aatcaattct gaaacaagga atcttgttgc agaggctgta tttaaagcat atgaagaaaa
     2521 gcgcacagaa gaaagcgaaa acgtggaaga agccgctctt atgtagcgct gttactgatg
     2581 atttacagtc aacagtactc gggtctttca tagacccttt tattgtgtct aaaggtaata
     2641 tgaatcaaaa ttggtgaatt tcctctatca atttttttta aatattagag taatgtccct
     2701 gaatattttt tgacttacta aatattatga tatataataa ctcagtatta cagattcgca
     2761 ttaaggagaa tttattatga gtatgtcaat aattttggca gcaggcgaag gtaccagaat
     2821 gaaatcagat ctaccgaaaa cgctgcataa agtatgtgga aaagaaatga taaaatacat
     2881 aattgaagca tctttaaatt caggtataga gaaaaatatt gttgttttgg gacatggaaa
     2941 agaaaaagtt ggacacaata ttaaggatat gaatgtacaa actgttgaac agcctatcgg
     3001 tgaagggcat ccctatggaa cgggctatgc cgttatggct gcagaaaacc acatcgatga
     3061 cggggaaacg gttttaatac tttgtgctga cggaccgctt gttaaaaaag aaacattaat
     3121 tgactttatg aactatcatt ttgaaagtaa ctgtgctgca tcgataatga cagcttattt
     3181 agataatcca aggggatacg gaagaatagt aagaaagtcg gatatgtcta tcgatgaaat
     3241 agtagaagaa aaggatgcat ctgaagaaat taaaaatata aaggaaataa atacggggat
     3301 atactgtttt aagggtgagc tcttaaaata tgctttgaag aaaattacca agaataactc
     3361 acagaatgag tattatctta cggataccat aaaggtttta aataatgaag gtgctaatat
     3421 aggagggtgg ctattagagg actcagatga tatcaaggct gtaaatgaca gggtacaact
     3481 tgctgaagta acaaaaataa tgcaaaaaag aataaatcaa agacatatgt attcaggtgt
     3541 tacaatcatt gacccggata gcacatatat agatgattcg gttaaaatag gcagagatac
     3601 ggttatttat cctaatacat atttggaggg aggaacggtt ataggaagca actgcacaat
     3661 cggacctaac tcaaggataa ttgattccac cgtaagaaat aatgtaaccg ttgaaagctc
     3721 aaaggttatt gaatcttata tcgatgacgg aacaaatgta ggaccatttg catatttaag
     3781 acccggcagc aatttaggta aaaatgtaaa aattggtgat tttgttgaag taaagaaatc
     3841 aatcattggt gataattcaa aatcatctca tttggcttac ataggtgatg cagaggtagg
     3901 taaaaatgtt aatataggat gcggagttgt atttgttaat tatgatggaa agaagaagca
     3961 taaaacaata gtcggcgacg gagctttcgt aggaagcaat tcaaatttag tcgctcccgt
     4021 tactattgaa aaaggcggat atgtggctgc aggctctacc attacggaag atgtcaaaga
     4081 gggagattta gcaattgcaa gagctaggca ggtcaataaa gaaggaagag gcaaaaacag
     4141 attttaaaga ggttaatatg tatgtaattg caggacttgg aaatccaggt aaggaatatg
     4201 caagctcaag acataatgca g
//
LOCUS       DAOSOW010000132         4217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_21090_length_4217_cov_4.149928, whole genome shotgun sequence.
ACCESSION   DAOSOW010000132 DAOSOW010000000
VERSION     DAOSOW010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21090_length_4217_cov_4.149928"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PLD67_04485"
     CDS             <1..754
                     /locus_tag="PLD67_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOT21810.1"
                     /translation="DINMQRTLVDQYFSRIINGFAGIIINTGEDNYLTTSDAYEEAHT
                     VLASQFINEQFALKAGLPEEQMGLGHAFEMNPMLENGFLYELAQAQMAREIFPKAPLK
                     YMPPTKYMTGNIFRGHLQDAMFNLISIWTHQGIQLLGMPTEAIHTPHLQDRMLSIENA
                     QYIFNNARDIGNEIEYKKDGIIQKRAQEVLAKAEELLKDIEKEGIFNTIEKGKFGGVK
                     RSFTGGKGLEGVVEKDEKYINPFIDLMLGGAK"
     gene            754..1512
                     /locus_tag="PLD67_04490"
     CDS             754..1512
                     /locus_tag="PLD67_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOT21811.1"
                     /translation="MEGIQKVDLTKIKPYGDTLNDGMVQMSFTLPIPYGDEASEAAKQ
                     LVSKMGFENPAVVFSKDLGVGYTYFIMYGKCTHTVDYTSIQVPKADIEFMQREEVEEY
                     IKENIKRDVVIVGACTGTDAHTVGIDAIMNMKGFDGNYGLERYKGIEAINMGSQVPNE
                     ELIAKAIEVKADAILVSQVVTQKNIHISNLTQLVEMVEAEGLRDKLILVCGGPRISYE
                     LAQELGYDAGFGTGSYANHVASFVVKQIVQRNLI"
     gene            1659..2819
                     /locus_tag="PLD67_04495"
     CDS             1659..2819
                     /locus_tag="PLD67_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOT21812.1"
                     /translation="MMKTKNILYFLKFNITIILFLSALYFENAHQPRLIVLMVIFVLF
                     LANNIAKYRLKAQNKILISFFVDIALIYILEANSRLLINYFFHSFYIIIFLEASLLLP
                     LRKGITIGIITVFISMIKYAYLIYYKFNLSNVSQMVFFLMVNILILVIAAFAQHTKDE
                     KEKKDILYRELLDTHKQLKESTDELKRLSAIEERNRIARDIHDTLGHNMTALIMQLQM
                     AEHYAKSDEVKSSQMLNNSLNTAKESLSKIREVVETLRGNEVTPKMEIKTLVKEFSEK
                     TGAEIELKIEGEISNNHDVNSAIYHILQEGMTNAIRHGNASKIWINLIYSNEYIKFNI
                     KDNGAGALAFIEGFGMKGIRERAESFGGNVEFKSQDGFIIDGFIPLVCHPER"
     gene            2971..3618
                     /locus_tag="PLD67_04500"
     CDS             2971..3618
                     /locus_tag="PLD67_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT21813.1"
                     /translation="MIKLLLADDQDILTEGLKLLLGAEEDIEIVGTANNGKKAYDLCR
                     IRKPDLVLMDIQMPEVNGVEATAMIKKDFPQTKIIVLTTFNDDEYIYDALKNGASGYM
                     LKDASPKEILEAVRTVYNGGALIQSEVAVKVIDQFSQLAKDTGDKHIDPNVGLLTDRE
                     IEICRLIAEGKNNKEIADELYLSQGTVKNYITKMLLKLDLRDRTQLAIFTIKNNL"
     gene            3871..>4217
                     /locus_tag="PLD67_04505"
     CDS             3871..>4217
                     /locus_tag="PLD67_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21814.1"
                     /translation="MKRKIIFVTVFILILLILLTSCNTNNLIEYKKASDKTEQIIKGQ
                     SAGEFTMTTEINPTGLTAEEIKELNYIKDMNGSFSVVFDDEEKKTIIRNYLNFGGLGY
                     DFEVFINGDEVSV"
BASE COUNT         1563 a          604 c          836 g         1214 t
ORIGIN      
        1 agatataaac atgcaaagaa cattggttga ccaatatttt tcaagaataa tcaacggctt
       61 tgcaggtatt atcataaata cgggagaaga caactatctg actacgtcag atgcttatga
      121 agaagctcac accgtattgg catctcagtt tattaacgag cagtttgcat taaaagcagg
      181 acttccagag gaacaaatgg gtctcggcca cgcttttgaa atgaacccga tgttagaaaa
      241 cggcttcttg tacgaattgg ctcaagctca gatggctaga gaaattttcc ccaaagcacc
      301 gcttaaatat atgcctccaa caaaatatat gacaggaaat atattcagag gacatttaca
      361 ggatgcaatg tttaatttaa tttccatatg gactcatcag ggaattcaat tgttggggat
      421 gcctacggaa gcaattcata caccgcatct tcaggacaga atgctttcaa tagaaaatgc
      481 acagtatatt ttcaataatg ctcgtgatat aggcaatgaa attgaataca aaaaagacgg
      541 aataattcag aaaagagcac aggaggtgct ggctaaagct gaagagctgt taaaggatat
      601 tgaaaaagaa ggaatattca atacaattga aaaaggtaaa ttcggtggtg taaaacgaag
      661 ctttaccggc ggcaaaggat tggaaggcgt tgttgaaaaa gatgaaaaat acattaaccc
      721 atttattgat ttaatgttgg ggggtgcaaa ataatggaag gtatccagaa agtagattta
      781 acgaaaatca aaccatatgg agatacatta aatgatggaa tggttcaaat gagttttaca
      841 ttgcccattc catacggcga tgaagcaagt gaagcagcga aacaattagt atctaaaatg
      901 ggatttgaaa atccggcagt tgttttttca aaagatttgg gagtaggata cacatatttc
      961 ataatgtatg gaaaatgcac tcataccgtt gactatactt cgatacaagt tccaaaggca
     1021 gatattgaat ttatgcaaag agaagaagtt gaagaatata taaaggaaaa tatcaagcgc
     1081 gacgtagtga ttgtaggagc atgcacaggt actgatgctc acaccgtcgg aatagatgcc
     1141 ataatgaata tgaaaggctt tgacggtaat tacggacttg aaagatacaa gggcattgaa
     1201 gcaatcaaca tgggcagcca ggtaccaaac gaagaactta ttgcaaaggc cattgaagta
     1261 aaagcagatg caatattggt atcccaagta gttacacaaa aaaatatcca tatttccaac
     1321 ctgacacaat tagtcgaaat ggtggaagca gaaggactca gggataagtt gattttagtt
     1381 tgcggaggac ctagaataag ttatgagctt gcacaggaat taggatatga tgcaggtttt
     1441 gggacaggaa gctatgctaa ccacgttgca tcatttgtgg taaaacaaat tgttcaaaga
     1501 aatttaatct aagagttata ttgagttgcc aggcacctga aaaggcgcct ggcacctttt
     1561 tttttatgtt ttctaaaaca tccattgcaa aaatacataa aaatggtaaa ataaataaaa
     1621 tagtaacagc tataaagttc acgttttacg gagtttttat gatgaaaacc aaaaatattt
     1681 tatattttct taaatttaat ataaccatca tcttattttt atcggcgctt tattttgaaa
     1741 atgcacatca accgaggctg atagtgctta tggtaatctt tgtgcttttt ttggccaata
     1801 atatagccaa ataccgttta aaagctcaaa ataaaatttt aatatcattt tttgttgata
     1861 tagcacttat ttatattttg gaagcaaatt caaggctttt aataaattat tttttccact
     1921 ctttttatat aataattttt cttgaagcat ctcttttact gccccttaga aagggaatta
     1981 caatcggaat tattacggta ttcatatcca tgataaaata cgcatatttg atttattata
     2041 aatttaattt gtccaatgta tcacagatgg tcttttttct catggtaaat atattgattt
     2101 tggtaattgc agcatttgca cagcacacca aggatgaaaa agaaaaaaag gatattttgt
     2161 acagagagct tttggatact cataagcagc taaaagaatc taccgatgaa ttaaaaaggc
     2221 tgtccgctat tgaagaaaga aacagaatag ccagagacat acacgatacc ttgggtcaca
     2281 atatgacagc cttgataatg cagcttcaga tggcagaaca ttatgcaaag tctgatgagg
     2341 taaaatcgtc acagatgcta aataactcat taaatactgc aaaagaaagc ttatctaaaa
     2401 taagagaagt tgtggaaact ttgaggggta atgaagttac accgaaaatg gaaataaaaa
     2461 ctttagttaa agaattttcc gagaaaaccg gtgcagaaat tgaattaaaa attgaaggag
     2521 aaatatcaaa caaccacgat gttaactcgg caatatacca catattgcag gaagggatga
     2581 caaatgcaat aagacacggc aatgcatcaa aaatttggat taatttaatc tattccaatg
     2641 agtatataaa atttaacata aaggataacg gagcaggagc tttggctttt attgaaggat
     2701 ttggtatgaa aggaataaga gaaagagctg aatcattcgg cgggaatgtc gaatttaaat
     2761 cgcaagacgg ttttataata gacggattta tccctttggt atgtcatcct gagcggtagc
     2821 gaaggatcta atgaatggca ttattctaac acagtaaaga atcctatatg cagtgtcatt
     2881 ctgaacgcag tgaagaattc tatatgcagt gtcattctga acgcagtgaa gaatctcatc
     2941 ttttaatggc aagggaattg gaggaaatta atgattaaat tactattggc agatgaccag
     3001 gatattttga ctgaagggct taagctgtta cttggtgctg aagaagatat tgaaattgta
     3061 ggtaccgcaa acaacggcaa aaaagcatat gatttatgca gaatcagaaa acccgatctt
     3121 gttctcatgg acatacaaat gccagaagta aacggtgttg aagcaacagc aatgataaaa
     3181 aaagattttc cgcaaacaaa aataattgta cttactactt ttaatgacga tgaatatatt
     3241 tatgatgcat taaaaaacgg tgcatcggga tatatgttga aagatgcttc accaaaggaa
     3301 attttagaag ccgtacgaac tgtttataat gggggagcat tgattcaatc agaggttgca
     3361 gttaaagtaa tagaccaatt ttcacagttg gcaaaagaca caggtgacaa acatattgac
     3421 cctaatgtag gacttttgac agacagggaa attgaaatat gccgccttat agcagaaggc
     3481 aaaaacaata aggaaattgc ggatgaatta tatctaagcc agggaacagt taaaaattat
     3541 ataacaaaaa tgttattaaa attagattta agagaccgta cccaattagc cattttcaca
     3601 ataaaaaata atttgtagaa gaatctcttt tttaaaacct gagatccttc gcttacgctc
     3661 aggatgacaa ttacagagga tattcgttta ttgtggtgtg gtaaacacgt gataattctg
     3721 agtgtattgg tagaatcttt ttttaaacag tgccaaaagt cacattattt tttcaagatt
     3781 tagtgattta aggtactatt atttaacttt ttattttaat attatgatac caatcaaagt
     3841 attaggatac aaagaattgg aggaatagaa atgaaaagaa aaataatttt tgtaacagta
     3901 tttatactta tattgctgat tttgcttaca tcttgcaata cgaataattt aattgaatat
     3961 aaaaaggcat ccgataaaac agaacaaatt attaagggtc agtcagcagg agaatttacc
     4021 atgactacgg aaatcaaccc gacaggattg acggcggagg aaataaagga gcttaattat
     4081 attaaggata tgaatggcag tttcagcgta gtatttgatg atgaagaaaa aaagactatc
     4141 ataagaaatt atttgaattt tggaggtttg ggatatgatt ttgaagtctt tatcaatggg
     4201 gatgaagtat ccgtaaa
//
LOCUS       DAOSOW010000133         4208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_21154_length_4208_cov_4.345052, whole genome shotgun sequence.
ACCESSION   DAOSOW010000133 DAOSOW010000000
VERSION     DAOSOW010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21154_length_4208_cov_4.345052"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..414)
                     /gene="yajC"
                     /locus_tag="PLD67_04510"
     CDS             complement(70..414)
                     /gene="yajC"
                     /locus_tag="PLD67_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOT21815.1"
                     /translation="MPQEYSGILMLVVFFAIMYFTIIRPQKKREKETKAMRDSLATGD
                     EVITIGGIHGKVVKINDEIVTLEMPFGKQRIEFSKWAIGSVTKKGKEKAAKEEVVKEE
                     IEETVDDKEDDK"
     gene            complement(435..1544)
                     /gene="tgt"
                     /locus_tag="PLD67_04515"
     CDS             complement(435..1544)
                     /gene="tgt"
                     /locus_tag="PLD67_04515"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOT21816.1"
                     /translation="MFKFELIKESKECKGRLGRIYTPHGIIETPVFMPVGTKGTVKAM
                     TPEELKELRAEIILGNTYHLYLRPGHELIREAGGLHKFMNWDGPILTDSGGFQVFSLG
                     NLRKIREEGVEFRSHIDGSKHFISPEVSMEIQNYLGSDIMMAFDECAPYPADYEYVKK
                     SMERTSRWAKRCKDYHKNTKSQALFGIVQGGMYKDLRIESAKQITDIEFPGYALGGLS
                     VGEPRDLMCEVMDYTVELLPKDKPRYLMGVGSPDYLFEAVERGIDMADCVLPTRMARH
                     GAFLTSKGQLTIKNAKYKNDFSPIDEECSCYTCRNYSRAYIRHLFGEKEILGARLASI
                     HNLFFLINLMKGIRISIAEDNFMKFKREFYENYGY"
     gene            complement(1552..2586)
                     /gene="queA"
                     /locus_tag="PLD67_04520"
     CDS             complement(1552..2586)
                     /gene="queA"
                     /locus_tag="PLD67_04520"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003451606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOT21817.1"
                     /translation="MTNFKTSDFYYELPERLIAQTPIKERDHSRLLVLDKKSGKIEHK
                     HFFNLVEYLNAGDCLVINDTKVLPARLFGNKKDTKTLIEILLLKRIDDKKWEILVKPG
                     KKARTGTEIVFDEKLLTGKIIDILEEGNRIIEFEYEGIFEEILDRLGEMPLPPYIHEK
                     LKDKDRYQTIYAKHLGSAAAPTAGLHFNDKLLEEIENKDVNIARLTLHVGLGTFRPVK
                     TEYINEHIMHSEYYELNESEANKINLAKSSGHKVITVGTTSVRTLETVADDEGIVNKS
                     KGWTNIFIYPGYKFKVVDSLVTNFHLPESTLIMLVSALAGKENIMKAYREAVEKEYRF
                     FSFGDAMFIK"
     gene            complement(2583..>4208)
                     /locus_tag="PLD67_04525"
     CDS             complement(2583..>4208)
                     /locus_tag="PLD67_04525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOT21818.1"
                     /translation="PTTAFGKNKDDIYVRVRIRYPRLFNEQVSFEGFDEIVLYDDEDE
                     LFCLNNSLTLRIDTYYSSNYYLSDKSQGTYGPYHAVLQDEIYSSYEEAYLIAEELNEE
                     FKVEFYPYLIEDGYIIAGGNFADKSSSQDLAEELNDEGYIAESMNGNLENIIAYNKKN
                     IPVFMYKKDMPVYFSSYNQDEEYEMIKVDNRAYRGRIRLNIIEDFKLLTINHVELENY
                     LYGVVPNEIPSSWGIEALKAQALAARTYAVYNIKPRQIYDMEDNQNSQVYMGYDYEKT
                     STNRAVDETRGEMIYYDGELIQAFYHSTSGGSTENSENVWYEKLPYLRGVNDEFSNNS
                     GSPHTTWQTTYYKEDIIRNLNLDGNDVDELYGIQIKKVSENNRVVECIFLTDAGEISY
                     KKENARLLLGLKSSWFSIVNGNIFCFITEYFYLDENRKDETTPSRGVLDLITEEETEE
                     TRNEIEFETGSIAGKHAITDDGIKKINREKLSFISSNGVTTVDANSEQYNFEGRGWGH
                     GIGMSQYGAKQMAEEGYTYEEIIKHYYTGVTVK"
BASE COUNT         1105 a          926 c          598 g         1579 t
ORIGIN      
        1 aacatttttg tcatgagatc cttcgctggc gctcaggatg acaattaaaa aaagagcatc
       61 agctcttttt tatttgtcat cttctttatc atcaacagtt tcttcaattt cttctttaac
      121 aacttcttct ttagctgctt tttctttgcc cttctttgta acacttccta ttgcccattt
      181 agaaaactca atcctttgtt taccaaaggg catttctaat gtaacaattt catcattgat
      241 tttaacaact tttccgtgaa taccgcctat ggttattacc tcatcccccg ttgccaagct
      301 gtctctcatt gctttagttt ctttttctct ctttttctgt ggtcttataa tggtgaaata
      361 cattatcgca aagaaaacga ccaacattaa aataccgcta tattcctgtg gcataattcc
      421 tcctatttac ttttttaata tccataattc tcataaaatt ctcttttgaa cttcataaaa
      481 ttatcttccg ctatacttat tctaatacct ttcattaaat taatcaagaa aaataaatta
      541 tgaattgatg caagcctagc gcccaatatt tctttttcgc caaataaatg ccttatatat
      601 gctcttgaat aatttctgca agtatagcag ctgcattcct catcaatggg actgaaatca
      661 tttttatatt ttgcattctt aattgtaagc tgtcccttgc ttgttaaaaa cgcaccatgc
      721 cttgccatcc ttgtaggcaa gacgcagtct gccatatcta ttcctctttc aacggcttca
      781 aacaaataat cagggctgcc cacacccatt aaataccttg gtttatcctt cggcaaaagc
      841 tctacggtat aatccataac ttcgcacatt aaatccctcg gctctcctac acttaagcct
      901 cctaatgcat accccggaaa ctcaatatcc gtaatctgtt tggcactttc aatccttaaa
      961 tccttgtaca ttcctccctg aacaattcca aacaaggctt ggctttttgt atttttgtga
     1021 tagtccttgc atctttttgc ccagcggctt gttctttcca tggacttttt aacatattca
     1081 taatctgcag gatatggtgc acactcatca aatgccatca ttatgtcaga gcctaaataa
     1141 ttttgtattt ccatggagac ttcaggactt atgaaatgct ttgaaccgtc gatgtgcgag
     1201 cggaattcaa cgccttcttc ccttattttt cttaaatttc ccagactgaa aacctggaat
     1261 ccgccgctgt cggttaaaat ggggccgtcc cagttcataa atttgtgaag ccctccggct
     1321 tctcttatta attcatgacc gggtcttaaa tacaagtggt aggtatttcc taatataatc
     1381 tcagccctta gctctttcaa ttcttcaggt gtcattgcct taaccgttcc ctttgttccg
     1441 acaggcatga atacgggtgt ttctataatc ccatggggag tatatattct ccccagtctt
     1501 cccttgcatt ccttagattc ttttattaat tcaaatttaa acataatcca cctattttat
     1561 aaacattgca tcaccaaagc tgaaaaatct gtattctttt tctactgctt cacggtatgc
     1621 cttcataata ttttcttttc ctgccaatgc gctaaccagc ataatcaaag ttgattccgg
     1681 aagatgaaag tttgttacta aactgtcaac caccttgaat ttgtatccgg gataaataaa
     1741 aatattcgtc catcccttgc ttttgtttac tattccttcg tcatcagcaa ctgtttccaa
     1801 tgttctgaca cttgtggtac ctacggttat aaccttgtgt cctgagctct tggcaagatt
     1861 gattttattt gcttcacttt cgtttaattc ataatactct gaatgcatta tgtgctcatt
     1921 gatgtattcg gtctttacgg gtctgaatgt tccaagcccc acatgcaagg taagccttgc
     1981 tatatttaca tccttatttt caatttcttc caaaagcttg tcattaaaat gaagtcctgc
     2041 cgttggagca gctgcagagc ccaagtgttt tgcatagata gtctgatatc tgtccttatc
     2101 cttaagtttc tcgtgtatat agggcggcag cggcatttcg cctaatctat ccaatatttc
     2161 ttcaaatatg ccctcatatt caaattcaat tattctgttg ccttcttcaa ggatatcgat
     2221 aatttttccc gtaagcaatt tttcatcaaa aacaatttct gtccctgttc tcgccttttt
     2281 accgggttta actaatattt cccacttttt atcatctatt cttttcagta aaagaatttc
     2341 aattaaagtc ttcgtatctt ttttatttcc gaataatctt gcaggcagaa ccttggtatc
     2401 gttaattact aagcagtctc ccgcatttaa atattcaact aaattaaaaa aatgcttatg
     2461 ctcgattttc ccgctttttt tgtcaagtac caaaagcctg gaatggtctc tttccttaat
     2521 aggtgtctgt gctatgagcc tttcgggcaa ttcgtaataa aagtcgcttg ttttaaaatt
     2581 cgtcatttaa ctgttactcc ggtataataa tgttttatta tttcttcata ggtatatcct
     2641 tcttcagcca tttgcttagc tccgtactgg ctcattccaa taccgtgtcc ccagcctctt
     2701 ccttcaaaat tatattgctc tgaattagcg tctactgttg ttacaccatt tgaacttata
     2761 aaggataatt tttccctgtt tatttttttt attccgtcgt cggttattgc atgcttacct
     2821 gcaatacttc cggtttcaaa ctctatttca tttcttgttt cttctgtttc ctcttctgtt
     2881 attaaatcaa gtactcctct tgatggtgtt gtttcatctt ttcgattttc atctaaataa
     2941 aagtattcgg ttataaagca aaatatattt ccgtttacta tagaaaacca cgagcttttc
     3001 aatcccaaaa gaagccttgc attttccttc ttatatgaaa tttctcctgc atcggttaaa
     3061 aatatacatt caactaccct gttattttca gaaactttct ttatttgaat tccgtacaat
     3121 tcatctacgt cattgccatc aagatttaaa tttcttatta tgtcttcctt gtaatacgtg
     3181 gtctgccagg tggtatgagg tgagcctgaa ttattggaaa attcgtcatt tacacctctt
     3241 aaatacggca gcttttcata ccatacattt tcggagtttt ctgtagaccc gccgctggtt
     3301 gaatgataga aggcctgaat taattcgccg tcgtaataaa tcatttctcc ccttgtttca
     3361 tccacagctc tgtttgtaga tgtcttttca tagtcataac ccatatatac ctgactgttt
     3421 tgattgtctt ccatatcgta gatttgtctt ggttttatgt tgtacaccgc ataggttctt
     3481 gcggccaaag cttgtgcctt caatgcttct attccccaag atgaaggtat ttcattcgga
     3541 acaactccat agaggtagtt ttcaagctct acatgattta tggttaaaag tttgaagtct
     3601 tcaattatat tcagtcttat tcttcctctg taggctctgt tgtcaacctt aatcatttca
     3661 tattcttcat cttggttgta cgaagaaaaa taaacaggca tgtccttttt atacatgaat
     3721 acaggtatat tctttttgtt gtatgcaatt atgttttcca ggttgccatt catcgattca
     3781 gcaatatagc cctcatcatt taattcttcc gctaaatcct gtgaagagct tttatccgca
     3841 aaatttcctc cggcaataat atatccgtct tcaatcagat aaggataaaa ctctacctta
     3901 aactcttcat ttaattcttc tgctatcaaa taagcttctt cataggaaga atatatttca
     3961 tcctgcagca ctgcatgata tggaccataa gttccttggc tcttgtccga taaataataa
     4021 ttgcttgaat aatatgtatc aattcttaat gtaaggctgt tattaaggca aaacagctca
     4081 tcttcatcat catacaatac gatttcatca aatccttcaa aagatacctg ctcgttaaaa
     4141 agtctcgggt atcttattct tacccttaca tatatgtcat ctttattttt accaaaagcg
     4201 gttgttgg
//
LOCUS       DAOSOW010000134         4200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_21211_length_4200_cov_4.172014, whole genome shotgun sequence.
ACCESSION   DAOSOW010000134 DAOSOW010000000
VERSION     DAOSOW010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21211_length_4200_cov_4.172014"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLD67_04530"
     CDS             complement(<1..234)
                     /locus_tag="PLD67_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOT21819.1"
                     /translation="MGAKENLMFCYQCQEAAGCKGCTKVGVCGKKPDTAGLQDLLIYA
                     SKGLSEVTTRLRNEGIEVSADVDHLITLNLFTTI"
     gene            546..1259
                     /locus_tag="PLD67_04535"
     CDS             546..1259
                     /locus_tag="PLD67_04535"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT21820.1"
                     /translation="MEKCNHCSHDCSHGYCIDNVAIFVDLPFEIKQDILDSSNHKTYK
                     KGEIIFNAGDYFDYLFIVIKGRVKLSKISAMGKEQILKILEVGDFMGELSLFKDTVMT
                     NFAEALENTEICIIRSEKIREIIMQKPEIAIKFLEKYAERIKHSEEIIEQIGLRDVEQ
                     RIANYLIAEVEKNNIKNRNNEYIINLPITKSVLSSILGTTKETLSRKLTLLQDEGLIR
                     MEGQRKIIITDIEGLRDMQ"
     gene            complement(1324..2499)
                     /locus_tag="PLD67_04540"
     CDS             complement(1324..2499)
                     /locus_tag="PLD67_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HOT21821.1"
                     /translation="MFKISDTVSWVGKIDWELNRFHGDEYSTHKGSSYNSYLIRDEKT
                     VLIDTVWEPFSKEFVANLKKEIDLNKIDYIIANHSEIDHSGALAELMEEIPDTPIYCT
                     KNGAKILKGHHHKDWNFVEVKTGDTLDIGKNKLIFVEARMLHWPDSMMTYMSGENILF
                     SNDAFGQHYASELMYNDKVDNDELYDEALKYYANILTPFSKFVVSKIEEVVALNIPIE
                     MICPSHGIIWRDNPLQIVNKYMDWAKSYQENQIAIVYDTMWNGTRRMAEAIAEGIKSK
                     DKDVDIKLFNCAKRDKNDIITEVFKSKMILVGSSTINSGILTATAGILEMIKGMKFTN
                     KKAAAFGSYGWSGEAVKIITEQLKEAKFEVVNDGIRELWNPDEEALERCREFGRNLV"
     gene            2693..3772
                     /gene="mnmA"
                     /locus_tag="PLD67_04545"
     CDS             2693..3772
                     /gene="mnmA"
                     /locus_tag="PLD67_04545"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOT21822.1"
                     /translation="MKKKVMVAMSGGVDSSVAAVLLKDQGYDICGATLKLFSDEDVLS
                     SCSRYRTCCSLEDVEDARSVCHKLGIEHFVFNFKETFQDEVIQKFKRKYEEGSTPNPC
                     IDCNKYIKFSKLIQRAILMGKDYIATGHYARIEFDNASGRYLLKRAVDLSKDQSYVLY
                     VLTQHDLSRTLFPLGGMLKAEVRKIAEERDLINARKPDSQDICFVRDGDYAGFLENMM
                     GVESKKGNFVDTEGNVIGEHKGIIHYTVGQRKGLGIALGKPVYVIDKNKELNTVTIGS
                     EEELYSKTLKATELNMIAVEKLTTTMKATVKTRYSQKETEALIHPDGEDSIIVEFIHK
                     QRAITPGQAVVFYDGDIVIGGGTII"
     gene            complement(3765..>4200)
                     /locus_tag="PLD67_04550"
     CDS             complement(3765..>4200)
                     /locus_tag="PLD67_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT21823.1"
                     /translation="SAFLMILGIIISYILSKYYILPISKGFDIIKNNPKEKVKTNIFE
                     IDELIEFLSSKTEIEKGTDPSSIILNEFLKNIKTLTPAEYSVFSLYARQYNAKEIAES
                     LCLSINTIKTHTKRIYSKLNINSKEELILYVEMLRESGRDIK"
BASE COUNT         1368 a          710 c          739 g         1383 t
ORIGIN      
        1 aatagtagta aataagttta aagtgattaa atggtcaaca tcggctgata cttctatccc
       61 ttcatttctt aatcttgtgg ttacttccga taatcctttt gatgcataga ttaataaatc
      121 ctgtaatccg gcagtgtcgg gtttttttcc gcatactccc accttggtgc agcctttaca
      181 gcctgccgcc tcctgacatt gataacaaaa catcaaattt tcttttgcac ccatataaat
      241 tgttctcctt tcaattttct tatatttgtt tttatgacac gattataata tattttaaaa
      301 atcacttctg taacatttgt tacaataatt tttaaagaac aattttttct gctctttttt
      361 tatacccgtc tttgtctata tataaacaat tataaatcaa aagtatttat tgtcccacct
      421 ttatcttatc ctgagcataa acccctatgt catcatgagc gtaagctgag gaatttaatc
      481 atcttttttg ttgaataaaa gtaagctaac tgttatgatt aattaaaaca gtctgggagg
      541 gtggcatgga aaaatgtaat cactgcagcc acgactgcag ccatgggtat tgtatagata
      601 atgttgccat atttgtagac ttgccttttg aaattaaaca agacatcttg gattcatcca
      661 accataaaac ttataaaaaa ggcgaaatta tatttaacgc cggagattat tttgattatt
      721 tgtttattgt tatcaagggg agggttaaac tctctaaaat ctctgctatg ggcaaggagc
      781 aaatactaaa aattcttgag gtgggagatt ttatgggaga gttgtcgctg ttcaaggata
      841 cggtgatgac taattttgcc gaggcattgg aaaatactga aatttgtata ataagaagcg
      901 aaaaaattcg tgaaattatt atgcaaaaac cggaaatagc aattaagttt cttgaaaagt
      961 atgctgaaag aattaaacat tcggaagaga taatagagca aataggttta agggatgtgg
     1021 agcaaagaat agcaaattac ctcattgcag aggtggaaaa aaacaatatt aaaaatagaa
     1081 ataatgaata tataataaac ctgccgataa ccaaaagtgt tttatcttcc atattgggaa
     1141 caaccaaaga aactctcagc cgtaaactta cccttttaca ggatgaaggg cttataagga
     1201 tggaagggca gaggaaaata ataattacgg atattgaagg attaagagat atgcaataaa
     1261 agagaaaatg aagaatctca tttttgagat tcttcactgc tttcagaatt accaagaatt
     1321 atattatact aagtttcttc cgaattccct gcatctttct aaggcttctt catcaggatt
     1381 ccaaagctcc ctgataccgt cgttgacaac ttcaaacttt gcttctttaa gctgctccgt
     1441 aataattttt acggcctcgc cgctccaacc gtagctgccg aatgcagcag cttttttatt
     1501 tgtaaatttc atacctttta tcatttccaa aattcccgct gttgcggtaa gaattccgct
     1561 gtttattgta gatgaaccta caagaatcat ttttgacttg aaaacttcag taattatatc
     1621 gttcttatct cgctttgcac agttaaacag tttaatatca acatctttgt ccttagattt
     1681 aataccttcc gcaattgctt cagccattct tcttgtaccg ttccacattg tgtcataaac
     1741 aattgcaatt tgattttcct gatagctttt agcccaatcc atatatttgt taactatttg
     1801 cagagggtta tctctccata ttattccatg gctgggacaa atcatttcaa taggtatgtt
     1861 taaagcaaca acttcttcta ttttgcttac aacaaatttg ctgaagggag ttaagatgtt
     1921 tgcataatac tttaatgctt catcatacaa ctcatcatta tctaccttgt cattgtacat
     1981 taactctgaa gcatagtgct ggccaaaggc atcattactg aaaagaatat tctctcctga
     2041 catgtatgtc atcattgaat ccggccaatg aagcattctt gcttcaacaa agataagttt
     2101 atttttaccg atatccaagg tatctcctgt ttttacttca acaaaattcc aatccttatg
     2161 gtgatgacct ttaagaattt tagctccatt tttggtgcaa taaataggtg tatcgggaat
     2221 ttcctccatt aattcggcta atgcacctga gtggtctatt tccgaatggt tggctattat
     2281 gtagtcaatt ttgtttaaat caatttcctt ttttaaatta gcaacaaatt cttttgaaaa
     2341 tggctcccag acagtgtcaa ttaagaccgt tttttcatcc cttattaaat aagaattata
     2401 agatgaacct ttgtgtgtgg aatattcatc gccgtgaaat ctgtttaatt cccagtcgat
     2461 tttacccacc cagcttaccg tatcgcttat tttaaacata cttcctcctt tgtttttaat
     2521 aataaatgca ctatattact taatatacca tatttacctt atttaaaaca agattatgct
     2581 aaatatttgt ttaaagtcta aattattgac aatgatattt tacacctata taatgaatta
     2641 ctgtaattat aataaattaa cgaaggggta taatacttta aaaggagttt ttatgaagaa
     2701 aaaggttatg gttgcaatga gcgggggagt tgactcatct gttgccgccg tcttgcttaa
     2761 agaccagggt tatgatatat gcggagcaac tttaaaattg tttagcgatg aagatgtatt
     2821 atcgtcttgc agcaggtata gaacatgctg ctctttagag gatgtggaag atgcgagaag
     2881 cgtttgtcac aaattgggaa tagaacattt tgtttttaat ttcaaggaaa cattccaaga
     2941 tgaagttata caaaaattca aaagaaaata cgaagagggc agtacgccaa atccatgcat
     3001 tgactgcaac aaatatataa aattcagcaa attgattcaa agagcaattt taatgggaaa
     3061 agattacatt gccacaggcc attatgccag aatagaattc gacaatgcaa gcggcaggta
     3121 cttattaaaa agggctgtgg acttgtcaaa ggaccaaagc tatgttcttt atgtgctgac
     3181 tcagcatgat ttgtcaagaa cacttttccc acttggcgga atgttaaaag ctgaggtcag
     3241 aaaaatagct gaagaaagag atttaataaa tgcaagaaag cctgacagcc aggacatttg
     3301 ctttgtgaga gacggtgact atgcagggtt tttggaaaat atgatgggcg tagaatctaa
     3361 aaagggtaat ttcgtagata ctgaaggaaa tgtaatcggt gagcacaaag gaatcatcca
     3421 ttataccgtg ggacagcgca aaggacttgg aattgcattg ggaaagccgg tctacgtaat
     3481 cgataaaaat aaagaactta atacggttac cattggaagc gaagaagagc tttattcaaa
     3541 aacattgaaa gctaccgagc taaatatgat agctgttgaa aaacttacca ccacaatgaa
     3601 agcaaccgtt aaaaccagat acagccaaaa ggaaactgaa gcattaatcc atcctgatgg
     3661 cgaagacagc attatcgtcg aatttattca caagcagcgt gcaataacgc ccggtcaggc
     3721 agtcgttttt tacgacgggg acatagtaat aggcggaggg acaattattt aatatccctt
     3781 ccggattccc taagcatttc aacgtaaaga attaattctt cttttgaatt aatatttagt
     3841 tttgaatata ttcgttttgt atgagtttta attgtattga tgcttaaaca taaactctcg
     3901 gcaatttcct tagcgttgta ttgccgggca tacaaactga aaacagagta ttctgcaggg
     3961 gtgagagtct tgatattctt cagaaattca ttgaggatta tcgaactggg gtctgtcccc
     4021 ttttcaattt ctgttttgct tgaaagaaat tctattaatt cgtcaatctc aaaaatatta
     4081 gtttttactt tttcttttgg gttgttttta attatatcaa aaccttttga tatgggtaaa
     4141 atgtaatatt tgctcaaaat atacgagata ataattccca gtatcattaa aaatgcgctt
//
LOCUS       DAOSOW010000135         4199 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_21220_length_4199_cov_3.884170, whole genome shotgun sequence.
ACCESSION   DAOSOW010000135 DAOSOW010000000
VERSION     DAOSOW010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21220_length_4199_cov_3.884170"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="PLD67_04555"
     CDS             complement(<1..633)
                     /locus_tag="PLD67_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT21824.1"
                     /translation="MKKFISLLVVMLLITSLVLTGCNEKPTEYQKVRLIEVTHSIFYT
                     PQYVALEMGLFEKQGLSIELTNGGGADKCMAALVSGEADIAFMGPEASVYVYLQGRDD
                     LAVNFAQLTQRDGSFIVGREKDDNFTIDKLKGHTILGGRKGGMPLMALEYVLKQNGLT
                     PGVDVNVRTDVQFDMMAGAFASGEADYTTLFEPLATSFELQGNGYVVESVG"
     gene            797..1849
                     /locus_tag="PLD67_04560"
                     /pseudo
     CDS             797..1849
                     /locus_tag="PLD67_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
     gene            1861..2478
                     /locus_tag="PLD67_04565"
     CDS             1861..2478
                     /locus_tag="PLD67_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOT21825.1"
                     /translation="MARKTDYFEGFIKLAEYSYNAAKLLDDTLRDFNKDSLQKTMKLM
                     HEIEHTADLEGHEITKKLLKEFITPIEREDIMLLIHKIDDITDCIEDVLLHMYMYNVR
                     VIKEDAIKFSGLILSSCEELIGALKEFKNFKKSKEIRSRIIAVNKIEEEGDNLYTEIV
                     RKLHLTSMNAIDIMTWTIAYNRLEKCCDACEEAANVIESIILKNL"
     gene            2581..3228
                     /locus_tag="PLD67_04570"
     CDS             2581..3228
                     /locus_tag="PLD67_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOT21826.1"
                     /translation="MDKHQKISSAVIRRLPKYYRYLGELNKVGITKTSSRELSEMTGF
                     SASQIRQDLNNFGGFGQQGFGYDVGNLRNEIGKILGLDKKYKIIIVGAGNIGQAIANY
                     TGFYEADYEVVALFDKNPKLVGLSIRNALIMDSDNLEEFLKKEHIDIAVICTPKSVSQ
                     QVAEQLVRCGIRAIWNFAPKDLKMPEEVYVENVHLNESLFSLTYYYNKMKKESGK"
     gene            3451..>4199
                     /locus_tag="PLD67_04575"
     CDS             3451..>4199
                     /locus_tag="PLD67_04575"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HOT21827.1"
                     /translation="MAKEVLNPLENSQMQVKKACDALGLHPDVYEILKEPQRVIEVNI
                     PVRMDDGSMKVFKGFRASHNDAVGPAKGGVRFHPGVTLEEVKALSIWMTFKGGVMGLP
                     YGGGKGGVICDPLTLSQGELERISRGYIQKLYKYIGEKEDIPAPDAGTNGQIMAWMVD
                     EYIKLTGGSASLGVITGKPVAWGGSEGRNEATGFGIAVITREAAKKYGIDLTKAKVAV
                     QGFGNVGRYTVKNIQRQGGTVVAVGEWAPKVG"
BASE COUNT         1429 a          629 c          869 g         1272 t
ORIGIN      
        1 tccaacggat tcaacaacat atccgttccc ctgaagttca aatgatgtag ccaacggctc
       61 aaacaaagta gtgtaatctg cttctccgga agcaaatgct ccagccatca tatcaaattg
      121 aacatccgtt cttacattta catccacgcc gggagtcaat ccattttgct ttaatacata
      181 ttccaatgcc attaagggca ttccgccttt tctgccgcca agaatcgtat gaccttttaa
      241 tttgtcaatg gtaaagttat cgtctttttc ccttccgaca ataaatgagc catcacgttg
      301 agtaagctgt gcaaagttaa ctgccaagtc atctcttccc tgtaggtaga catagacgct
      361 tgcttcaggt cccataaaag ctatatcggc ttcgccgctt accaaggccg ccatacattt
      421 gtcggcacct ccgccgttag tcaattcaat acttagacct tgcttttcaa ataatcccat
      481 ttcaagtgca acatactgag gtgtataaaa aatcgagtga gttacttcta taagccgtac
      541 tttttgatat tccgtgggtt tttcattgca tcctgtaagt actaatgatg taatgagcag
      601 cataacaaca agcagagaaa taaacttttt catttcatcc tcccaaaatt agtctttcga
      661 atataatatt caatttatct ctaaagcgtt acataactct ttataaaagt agtggaaaag
      721 taatggattc tatggtatat tatctttgta tgcaatatat atataataaa aacatgagta
      781 gcagacggag aataatatgg ctatatcttt ttcggattat ttaatgcaga ttactgataa
      841 ccccatgttg cttattactt cgattttaac catggcggtt atcttggtca atggttggac
      901 tgatgcacca aacgctattg ccacatgcgt ttctacccgt tccatatcag cgaaatcagc
      961 gattgtaatg gctgcaattt ttaatttttt gggtgtttta ttgatgacag taattaatgc
     1021 aaatgtggca caaacaattt ataacatggt tgactttgga ggtaattcat atgatgcact
     1081 ggttgcttta tgtgcagcat tatttgcaat cgtaatatgg gctaccgctg catggtggtt
     1141 tggaattcca acaagtgaaa gccatgcact gatagcgggt atatcaggtg ctgccattgc
     1201 cttgcataac gggttacaag gaataaacgg cagtgaatga attaaggtat tgtatggatt
     1261 aggtttatct acctttttag gctttaccct aggttggatt gttgtaagga tggtagagat
     1321 tatttttagg tcaatgagca ggatgaaagc caatatcctt tttacaaatg ctcaagttgc
     1381 aagcggagca gctatggcat ttatgcacgg tgctcaagac ggacaaaagt ttatgggagt
     1441 atttatgctt ggtatttatt tagcaggtgg acaagcaaat gtaaccactt ttcaaatccc
     1501 tttatggatg atgatattat gctcagctat tatgggcatc ggaacatcaa taggtggtta
     1561 taggataatc aaatcagttg gaatggatat gtgcaggttg caaaaatatc aaggtttttc
     1621 tgcggatatg gctgctgcca cttgtttgtt tatttcttca atgaccggaa taccggtaag
     1681 cactactcat actaagacta ctgcaataat gggtgtggga gcagcaagaa gattttcttc
     1741 agtaaattgg ggaattgtcg gtgaaatggt tatgacatgg attttgacat tcccgggatg
     1801 tggaataatc ggatatttaa tggcgaaatt atttataaat atattttaat gaggtgaaat
     1861 atggcaagga aaacagatta ctttgaaggc tttataaaat tagccgaata cagctataat
     1921 gctgcaaagc tcttagacga tacattgagg gactttaata aagattcttt acagaaaacg
     1981 atgaaattaa tgcatgaaat agagcataca gccgatttgg aaggtcatga aataactaaa
     2041 aagcttttaa aggaatttat tacccctatt gaacgtgaag atattatgct tttgatacac
     2101 aaaattgacg atattacaga ttgtattgaa gatgtgctgc ttcatatgta tatgtataac
     2161 gtaagggtta tcaaagagga tgcaataaaa ttttccggat tgattttaag cagttgtgaa
     2221 gaattaatcg gagcattaaa agaatttaaa aatttcaaaa aatcgaagga aatacgaagc
     2281 agaattattg ctgtgaataa gattgaggaa gaaggagata atctttatac agaaattgta
     2341 agaaagctgc atcttacatc aatgaatgcc atagatatta tgacatggac aatagcctac
     2401 aatcgacttg aaaaatgttg tgatgcttgt gaagaagctg ctaatgtcat agaaagtatt
     2461 attttgaaga atttataagc gaaaagataa agattgttaa aaaattctct atatttgtta
     2521 aaaaatgtgt tgcaatataa atgcaagatt gataaaatat atccatagga ggcagcatat
     2581 atggataaac atcaaaaaat ttcatctgca gtaataagaa ggttacctaa atattatagg
     2641 tacttagggg aattaaacaa agttggaatt accaaaactt cttcaagaga actaagtgaa
     2701 atgacaggtt tctctgcttc acaaataaga caagatttaa ataactttgg gggattcgga
     2761 cagcagggat ttggctacga tgtgggaaat ctgcgtaatg aaattggaaa aatactagga
     2821 ttagacaaaa agtataaaat tataattgtt ggtgcaggaa acataggcca ggcaattgct
     2881 aattatacag gtttttatga agctgactat gaagttgttg cgttgtttga taaaaatccg
     2941 aagcttgtag gattatctat cagaaatgca ttgataatgg attcagataa tttagaagaa
     3001 tttcttaaaa aagagcatat agacattgca gttatctgca ctccaaagag tgtaagccaa
     3061 caagttgccg agcaattggt gcgttgcggc ataagggcta tttggaactt tgctccaaag
     3121 gatttaaaaa tgcctgaaga agtatatgtt gaaaacgttc atttaaatga aagtttattt
     3181 tctctaactt attattacaa caagatgaaa aaagaaagcg gaaaataatg cataataagc
     3241 agaagtctag gcttctgctt ttgcattcaa tggttttttg tatgaaaaaa atttatatta
     3301 ataaattttc ttgttagtgc aaataatatt ttttatcctt gaaataattt gaaatgggtg
     3361 atagaataag attgtccgat aatattgtta ttaatttagc aatattaaag ataaaaatac
     3421 ttataaacta ataaatttgg aggtatagta atggcaaaag aggttttaaa tccattggaa
     3481 aactctcaaa tgcaagtaaa aaaagcttgt gacgctttag gattgcaccc ggatgtatat
     3541 gagattttaa aggaaccaca aagagtaatt gaagtcaaca ttcctgttag aatggatgat
     3601 ggaagcatga aagtattcaa aggcttcaga gcatcgcata atgatgctgt tggtcctgca
     3661 aaaggcggag ttagattcca tccaggcgta acattggaag aagtaaaagc tctttctatc
     3721 tggatgacat ttaaaggcgg agtaatgggt cttccttatg gcgggggtaa aggtggagtt
     3781 atttgtgatc cattgacatt atctcaagga gaattagaaa gaatttctag aggctatatc
     3841 caaaaactgt ataagtacat aggagaaaaa gaagatatac cggcacctga cgcaggaaca
     3901 aacggacaaa ttatggcttg gatggtggat gaatatatca aattaaccgg cggctctgca
     3961 tcattaggtg ttataaccgg aaaacccgta gcatggggcg gctcagaagg cagaaatgaa
     4021 gcaacaggtt ttggtattgc agtaattacc agagaagcag caaaaaaata tggaattgat
     4081 cttacaaaag caaaagttgc cgtacaggga tttggtaacg taggtcgtta cacagttaaa
     4141 aatattcaga gacaaggcgg tacagtagta gctgtaggag aatgggcacc aaaagttgg
//
LOCUS       DAOSOW010000136         4196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_21239_length_4196_cov_3.702970, whole genome shotgun sequence.
ACCESSION   DAOSOW010000136 DAOSOW010000000
VERSION     DAOSOW010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4196
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21239_length_4196_cov_3.702970"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1160
                     /locus_tag="PLD67_04580"
     CDS             57..1160
                     /locus_tag="PLD67_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009327843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HOT21828.1"
                     /translation="MNFLQLDTPALLIDKDIMLNNLKHMQDYANEKKVALRPHTKTHK
                     MSKLALLQEELGAKGITVAKVGEAEVMAANGLRDIFIANEIVGETKLSRVKKLAETID
                     ISFGLDSIAQADMVEKVFEGSKRANVLIEIEVGENRSGVIEEDDYIELLNYLKQCSNI
                     NLKGVFSHDGHSYSAENIEACKEIHLEAQRRTLRFANIANEMGFKLETVSIGSTPSLM
                     HNFPILEGVTEIRPGTYIFMDASQANAYGNVDRNAATILTTVISLPTPERVITDVGAK
                     GITMQARSKGFTATKGLGLIKGWPEVSIYAVYDEHAIIYNKKFHDSMKVGDKVEIIPN
                     HICPVVNLYETAYIIENGRVVDEIPVDCRGKLK"
     gene            complement(1224..2144)
                     /locus_tag="PLD67_04585"
     CDS             complement(1224..2144)
                     /locus_tag="PLD67_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOT21829.1"
                     /translation="MKTKLMADSTCDLSQEILNRYNICLAPLVVSIEGKEYRDRIDIS
                     NDEFYKNLMTFKPYPTTSMPSPESYIACIKHAIEQGYEKILCICMSSGTSGSYQSALL
                     AKELFYEQNPKSNIQFEIIDSVSMSHGSGWLIMKCAMLIENGYTFEELIEYCEIIKYR
                     IKHFLSVDDLDSLIRSGRLSNTSGVIGKLLNVKPIMSMKNKKGTVVAKKRGIKQVYNY
                     YIQEFINRVDYEQTDFVIIGYTSDINKAENLKALLLKANYKGDIYIMQMGVAVGTHVG
                     LGGLSMFFVEKPHEDFINHMKEKIISHIHS"
     gene            complement(2303..2923)
                     /locus_tag="PLD67_04590"
     CDS             complement(2303..2923)
                     /locus_tag="PLD67_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT21830.1"
                     /translation="MEKKNINKRKLKAIETKNKIYEAARKLSLEHGIEKVSVDTIVEA
                     AGVSKGAFYVHFESKDALTAELVDEYTNKADIDYKAFFNSLPDNMPILDILVLLAEEI
                     CDFIETNIGLENMRVLYKAHLTKTINTSSAMSYNRELYNLFTEVLEKGLMRGEIRQDI
                     PVNNMAKHLVLAIRGITYEWCIRYPDIDLKEYVLDHFKILLYGLES"
     gene            3106..>4196
                     /locus_tag="PLD67_04595"
     CDS             3106..>4196
                     /locus_tag="PLD67_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT21831.1"
                     /translation="MKKGIILLLIITLILSLFQTAFAQIYDISNHWASKEINYLVERE
                     VLSGYPDGTFKPDNPITKAEFYKVINKLLNFEERADITYSDVNETDWYYEDVGKALAA
                     GYISPATVLLPNEYITRQEVARILGFVYNLDDESEAASVFSDSESILPEIRGYIGVLF
                     ENNLLHGYPDGTFKPSANITRAEVVKLIYNVLEKLNNLPIKVSANESEKVSDSSHRDT
                     SVKRYTLTEGTNEITTHVKIRTGTVIGNVTIEYTGSADLKLPAGLTVDGDLTVIAPKA
                     TVNNYASVTGNIIIRDVASTTWNEYADGNILIVEDENGLVLHVVGNVRGITVSESTNG
                     NITIALSRDVNTDAIEFIAINGPVTLITQ"
BASE COUNT         1409 a          676 c          774 g         1337 t
ORIGIN      
        1 atgggtttgg gcatttaaca aatgcctaaa tatcagttaa agtaaggaga aaaaacatga
       61 actttctaca attagataca cctgctttgt taatcgataa ggatattatg ttgaataatt
      121 taaaacatat gcaggattac gcaaatgaaa aaaaagtagc attaaggcct cacaccaaga
      181 cacataaaat gtctaaatta gctttattgc aggaagaatt gggggcaaaa ggcataacgg
      241 tggctaaggt tggtgaagcg gaagtaatgg ctgcaaacgg actaagggat atttttattg
      301 ctaatgagat tgtaggcgaa acgaagcttt cacgtgttaa aaaactggct gaaacaatag
      361 atatttcatt tggacttgac agcatagccc aggcagatat ggtcgagaaa gtattcgagg
      421 gcagcaaaag agcaaatgta ttaattgaaa ttgaagtggg agaaaaccgc tccggagtta
      481 ttgaagaaga tgattacata gagcttctaa attatttgaa acaatgcagc aacataaatt
      541 taaagggagt tttttctcac gatggtcatt cttattcagc cgaaaatatt gaagcatgca
      601 aagaaataca tttggaagct caaagaagaa ccctgcgatt tgcaaatatt gcaaatgaaa
      661 tgggatttaa attagaaaca gtcagtatcg gctcaactcc ttctttaatg cacaattttc
      721 ctatattgga aggagtcact gaaatcagac cgggcacata tatttttatg gatgcttctc
      781 aagctaatgc atacggaaat gtggacagga atgcagctac aatcttaact actgtaataa
      841 gtctacctac acctgagagg gttattacgg atgtcggtgc caagggaatt acaatgcagg
      901 caagaagtaa gggattcact gcaacaaaag gcttgggtct tataaaagga tggccggaag
      961 taagcattta tgctgtatac gatgaacatg caattatata taacaagaaa tttcacgatt
     1021 caatgaaagt cggagataag gtagagataa ttcccaatca tatatgtcct gtggtaaact
     1081 tgtatgaaac agcctatatt attgagaatg gccgtgtggt ggatgagatt cccgttgatt
     1141 gcagaggaaa gcttaaatag gataagtgaa cgtaaatagt tgaatttaaa ataaagggga
     1201 tgtgcttaac atccccttta tcactaacta tgaatatgac ttattatttt ttctttcatg
     1261 tgatttatga agtcttcatg aggtttttcg acaaagaaca tggaaagacc gcctaaacct
     1321 acgtgagtac caacagctac tcccatttgc ataatgtata tgtcaccctt atagtttgct
     1381 ttaaggagca gggccttcaa attttctgct ttattaatgt cggatgtata tccgatgata
     1441 acaaaatcag tttgttcata atctacccgg tttataaatt cttgaatata ataattataa
     1501 acttgtttaa ttcctcgttt ttttgccact acagtgcctt ttttattttt cattgacata
     1561 atcggcttga catttagcaa tttgcctata acgccgcttg tatttgataa ccttccgctg
     1621 cgtatcagac tatccaaatc atctacggat aaaaagtgtt tgattctgta ttttattatt
     1681 tcacaatatt caataagctc ttcaaacgta tatccgtttt caataagcat ggcgcatttc
     1741 ataataagcc atccgctacc atgactcatg ctaaccgagt ctattatttc gaattgaata
     1801 ttagactttg gattttgttc gtaaaataat tcttttgcca gtaaagcgct ttgataagac
     1861 ccgcttgttc cgcttgacat gcaaatacag agtattttct catatccttg ctctatggca
     1921 tgtttgatac aggcaatata tgattcagga cttggcattg aagtggtcgg atatggtttg
     1981 aaagtcatca ggttcttata aaattcatcg tttgaaatat ctattcggtc tctgtattcc
     2041 tttccttcta ttgaaacaac cagtggagct aaacatatat tgtaacgatt taaaatttct
     2101 tgagataaat cacacgttga atctgccatt aattttgtct tcatataagc tcccgttctt
     2161 atagtatttt agtgcttgta tatttgcact atcataccat cctattcgac aatattcaac
     2221 atttttcgta attctttgaa atacaatttt tagcagctcg tatattaaat tggtaagcat
     2281 atacaagaga tatattgcat ttttaacttt ctaacccgta aagcagtatt ttaaagtggt
     2341 ccagaacata ctccttcaaa tcaatatcgg gatatcggat acaccattca tatgtaatgc
     2401 ctctgatagc taagaccaag tgtttcgcca tattattaac aggaatgtct tgtcttatct
     2461 caccccgcat gagccctttt tccaatactt cagtgaacaa attatacagc tccctgttat
     2521 aactcattgc agatgatgtg tttatagtct ttgtcaagtg agctttataa agcactctca
     2581 tattttctaa tccgatattt gtttcaataa aatcgcaaat ttcttctgcc aataaaacaa
     2641 ggatatcaag gataggcata ttatcaggaa gagaattgaa aaaggcttta taatcaatgt
     2701 ctgctttgtt tgtatattca tctacaagtt cagctgtaag cgcatctttt gattcaaagt
     2761 gtacatagaa tgcacctttg gatactcctg ccgcctctac aatggtatca acacttactt
     2821 tttcgattcc atgctccaaa gataattttc gggcggcttc ataaatttta ttttttgttt
     2881 caattgcttt caattttctt ttgtttatat tttttttctc cattcttccc tccttgtgat
     2941 tgactgattg gaacaaatat tgtataatga ctacggtatg tgactgcagt cattatactg
     3001 cattttttaa attataacat atattccatt ttttgtatag atggatatta aaaaagaaac
     3061 tcttaagttt cacttattag acaagtcata ataggaggaa gatttatgaa aaaaggtatt
     3121 atattgctac tgattattac gcttattcta tctttattcc aaacagcatt tgctcaaata
     3181 tatgatataa gtaaccattg ggcaagcaaa gaaattaatt atttggtaga gagagaggta
     3241 ttaagcggat acccggatgg aacttttaaa ccggataatc caataacaaa ggcggagttt
     3301 tacaaagtaa ttaataaatt attaaatttt gaagaaagag cagatattac atattcagac
     3361 gtaaatgaaa cagactggta ttatgaagat gtgggaaagg cattggcagc aggatacatc
     3421 agccctgcaa cagtattgtt gccaaatgaa tatataacaa ggcaagaggt agcaagaatc
     3481 ttaggatttg tttataatct tgatgatgag tcagaagctg caagcgtatt ttcggattct
     3541 gagtcaatat tgcctgaaat aagaggttat attggtgtat tattcgaaaa caatctatta
     3601 catggatatc cggatggaac atttaagcct tcagcaaata ttaccagagc tgaagtagtt
     3661 aagctgattt acaatgttct tgaaaagctg aataatttac cgataaaggt aagtgccaat
     3721 gaatcagaga aggtatctga ttcatctcac agggatactt cagtaaaaag atatacatta
     3781 acggaaggaa caaatgaaat aactacacac gttaaaataa gaacaggtac agtgattgga
     3841 aacgtgacaa ttgaatacac cggcagtgca gatttaaaat tacctgccgg attgactgta
     3901 gatggggatt taaccgtaat agcaccaaag gctactgtaa acaactatgc aagcgtaacc
     3961 gggaatataa taatccggga tgtagcaagc acaacttgga atgaatatgc agatggcaat
     4021 atattaattg ttgaagatga aaatggctta gtattacatg tagttggaaa tgtcaggggt
     4081 attacagtaa gcgaatcaac aaatggaaat ataacaattg ctttaagtag agatgtcaat
     4141 acggatgcta tagaatttat tgcaatcaat ggacctgtta cattaataac gcaaaa
//
LOCUS       DAOSOW010000137         4190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_21290_length_4190_cov_3.832890, whole genome shotgun sequence.
ACCESSION   DAOSOW010000137 DAOSOW010000000
VERSION     DAOSOW010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21290_length_4190_cov_3.832890"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(386..2173)
                     /locus_tag="PLD67_04600"
     CDS             complement(386..2173)
                     /locus_tag="PLD67_04600"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HOT21832.1"
                     /translation="MKKLTDNSNVLKWVDEIISMTNPSNVVWIDGSEELYEKLRKEGC
                     STGELVKLNEEIYPGCYYHRSAKDDVARMEDKTFICSRKKEDAGNTNNWKSPEEAYKL
                     GREIFTDSMKGRTMYIIPYSMGPVGSPFSKIGIEITDSIYVVLSMNIMTRVGKEVLDA
                     LGDSNDFVRGVHGKCTLDINKRYIFHFPEDNTIWSCNSGYGGNVLLGKKCFALRIASY
                     QGRMEGWMAEHMLIVGIENPQGEVKYIAGAFPSACGKTNLAMLIPPEIYLNKGYKVWT
                     VGDDIAWLRIKEDGRLWAINPEAGFFGVAPGTNAKSNYNALMSTKKNSIFTNVVLNLD
                     DNTVWWEKLDNNPPENALDWMGNPWNGKTSKENGAHPNSRFTAPAKNCPCISPEWESP
                     EGVPLSAIIFGGRRAKTAPLVYQSRDWQHGTFVGSILASETTAAASGKVGVVRRDPMA
                     MLPFCGYNMGDYFKHWLEMGKKMSNPPKIFHVNWFRTNEEGQFIWPGFGDNFRVIEWM
                     LKRCENKVDANEAPIGYLPKPEDINIEGLNLSADTLKSLLSIDTESWLADIKNIEEFY
                     AKIGDAIPQELIEELRTLADNLIRSAALV"
     gene            complement(2396..3742)
                     /locus_tag="PLD67_04605"
     CDS             complement(2396..3742)
                     /locus_tag="PLD67_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOT21833.1"
                     /translation="MDRNLVLKPSAIMSICLIFIIGSIILSIPLYFGFLAGIVLTSTV
                     LYKSGYHMREIYETSVSALKDVKRLLIVILFIGATTSIWLASGVVPTIMYYGFTYMEG
                     VNFLLAVFLITSVVSYFLGTAIGTISTVGISLSGIGLSIGIPHNLMVGVLVSGAFIAD
                     KISPLSGLVNLGMTTVNRNYKELFKSMIITLIPAIAATSVIYFIIGSSYIAKTENLII
                     YKQAIQEGFNTSPALFILPAVILILSISGLDSLKTFSIGIGVGAILSIIFQQESLLSI
                     IKALIFGYRGNTPSEELNRLLLSGGMISMIEAIFIVAGAYLLIKLFEKGNVLVPLMDK
                     LVYGINNKMSLIGRTGLISILMTVATCDQTSGIILPGTILQEKYKELKLDNAVLARTI
                     FDTGVIVAPLMPWNINSFLIKPVMGITAAQYAPFAVLCIVCPLITMLVSYFQYRNS"
     gene            complement(3747..>4190)
                     /locus_tag="PLD67_04610"
     CDS             complement(3747..>4190)
                     /locus_tag="PLD67_04610"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HOT21834.1"
                     /translation="KIGAFIESKMKNKESNVALGFVACTLVYCVGSMAIVGSIQSGLT
                     GNHEILFSKAVLDGITAVTFAATMGVGVVFSGISVLVYQGTITMLASIMQSLLNPVVV
                     SEMTAIGGVIIMGIGLNFLISNRMRVGNMLPSIFIPIIYYMILMR"
BASE COUNT         1284 a          880 c          649 g         1377 t
ORIGIN      
        1 aaatactttt ctctgcttca acgctttaaa tattaatagc aataataact tataataatc
       61 cctttgtcaa caattatttt tcagtaatat tttaccaatc ataaaaataa cgagggctac
      121 gccctcgtaa actgttgtat tcctatttga gattgtccga ttatttaaag aaacataact
      181 aaaaatatac gctcacggtt tcggccacga ataattctaa agctcattcc ggctaaaaat
      241 cctacagctt gcaaactcgc tgcgctcaaa caatgcaagc tgcttaacgg atttttaacc
      301 ttcattcgct aagaattata gtcgtgtcct gcaaatgttc gctaaatatt tttagttatt
      361 atattgttga ttgctctgta taacattaaa ccaatgctgc acttctgatt aaattatcag
      421 ctaaagttct aagttcttca attaattctt gaggtatcgc atcacctatt ttagcataga
      481 actcttctat attttttata tctgcaagcc atgattcggt gtctattgaa agaagtgatt
      541 tcaatgtgtc tgctgataaa ttaagacctt caatattaat gtcttcgggt tttggcagat
      601 aaccgatagg tgcctcattt gcatccacct tattttcaca tcttttgagc atccattcta
      661 ttaccctgaa attatctcca aaccctggcc aaataaattg cccttcttca tttgttctaa
      721 accagtttac atggaaaatt tttggcggat tgctcatttt tttacccatt tcaagccagt
      781 gtttaaaata atctcccata ttgtatccgc agaatggaag catggccatg gggtcccttc
      841 ttacaactcc gacttttcct gatgcagctg cagtagtttc cgatgcaagt atcgagccta
      901 caaaagtacc atgctgccag tcccttgact gataaacaag gggagcagtc tttgcacgcc
      961 ttcctccgaa tattattgcc gataaaggaa ctccctccgg tgattcccat tcaggtgaaa
     1021 tgcacggaca atttttagcc ggtgccgtaa atcttgagtt aggatgtgct ccgttttcct
     1081 ttgatgtttt cccgttccat ggatttccca tccagtccag agcattttca ggaggattgt
     1141 tgtcaagttt ttcccaccat accgtgttat cgtccaaatt caatacaaca tttgtaaaaa
     1201 tcgaattttt ctttgtagac ataagagcgt tataatttga ttttgcattt gttcccggtg
     1261 caactccgaa aaagcctgct tcaggattta tagcccatag tcttccgtct tctttaattc
     1321 gaagccatgc tatgtcatcg cctacagtcc aaactttata tcctttgttt aagtaaattt
     1381 caggaggaat aagcatagcc aaattcgttt tgccgcatgc tgacgggaat gctcctgcta
     1441 tgtattttac ttctccctga ggattttcta ttcctacaat gagcatgtgc tcagccatcc
     1501 aaccttccat tcggccctgg tatgacgcaa tgcgaagtgc aaagcatttt ttgccgagga
     1561 gaacattgcc cccgtaaccg gaattgcatg accatattgt attgtcttcg ggaaaatgga
     1621 aaatatatct tttgttgata tccaatgtgc actttccgtg tacgcctctc acgaaatcat
     1681 tggaatctcc taatgcatca agtacttctt ttccaactct cgtcattata ttcatgctta
     1741 aaacgacata aatagaatcg gtgatttcaa ttcctatctt tgaaaagggc gaaccaacag
     1801 gccccattga atagggaata atatacattg ttcttccctt catggaatca gtgaaaattt
     1861 ctcttcccaa tttataagcc tcttcagggc ttttccaatt gtttgtatta cctgcatctt
     1921 ccttttttct ggaacaaata aatgttttgt cttccattct tgctacgtca tcttttgccg
     1981 agcgatggta atagcatccg gggtaaattt cttcgttcaa ttttacgagt tcgcctgttg
     2041 agcatccttc tttgcgaagt ttctcgtata attcttcact gccgtcaatc caaactacat
     2101 tcgatggatt agtcatagaa attatttcgt caacccactt taaaacattt gaattgtccg
     2161 ttaatttttt catggcactc tcctaaatat tatttgtgtt actatgtttt attaattgta
     2221 accactatat cacaagtgta ttatatagct actgtataat acattcaata tcaaaaacaa
     2281 attcgaagat tcttaacttt tattatgctt tacttaaaat aaaactgtat tttttaaaaa
     2341 agcaaaataa ttgtaacact gtagtacaat aagagaatca tgaaacaaat ttcggttatg
     2401 aattcctata ttgaaaatag gaaaccagca ttgtaatcaa gggacaaaca atacataata
     2461 cagcaaatgg agcatattgg gctgcagtaa ttcccattac gggttttatt aaaaatgaat
     2521 ttatattcca aggcattaga ggtgccacaa taactcccgt gtcaaatatg gttcttgcca
     2581 agacagcatt gtccaatttc agctccttgt atttttcctg caatatggtg ccgggtaata
     2641 taattcccga tgtttgatca catgttgcca ctgtcatcaa tatacttatc agtcctgttc
     2701 tgcctattaa actcatttta ttgtttattc cgtaaaccag cttatccatt aaaggcacaa
     2761 gcacattgcc cttttcaaaa agtttaatca ataaatatgc tccggcaact atgaatattg
     2821 cctctatcat tgatatcata cctccgctga gcaagagtct gtttaattct tcggaaggtg
     2881 tgttgcctcg ataaccgaat ataagcgcct taataattga taataacgat tcctgctgaa
     2941 atataatact aagtattgca cctaccccaa taccgataga aaatgttttt aatgaatcaa
     3001 gtcccgaaat ggataaaatt agaattactg caggtaaaat aaataatgcc ggcgatgtat
     3061 taaatccttc ttgaattgcc tgtttataaa ttataagatt ttctgtttta gcaatataac
     3121 ttgaacctat aataaaatat attactgatg ttgctgctat agccggtatc aatgttataa
     3181 tcatactctt gaacagttct ttatagtttc tgttgacggt agtcattccg agatttacca
     3241 aacccgacaa aggagatatc ttatctgcta taaaagctcc cgaaaccaag acgccaacca
     3301 tcaaattatg aggtattcct atacttaatc cgattccgct taaggatatt ccaacggtgc
     3361 ttatagttcc gattgccgtg cccaaaaaat atgaaacaac actcgtaatt agaaataccg
     3421 ccaaaagaaa gtttactccc tccatatatg taaaaccata gtacataatt gtaggcacaa
     3481 cacctgatgc aagccaaatt gaagtagttg cgcctataaa caatattaca atcaataaac
     3541 gtttaacatc ctttaatgca gacacagatg tttcgtaaat ttcccgcata tggtaaccgc
     3601 ttttataaag aactgtagaa gtaagaacaa tccccgccaa aaaaccaaaa tataggggga
     3661 ttgataaaat aattgagccg ataataaaaa ttagacatat agacattata gcacttggtt
     3721 ttaaaactaa atttctatcc aatttattac ctcatcaaaa tcatatagta tattataggt
     3781 ataaatatag acggcagcat attacctacc ctcattctgt ttgatattag aaaattcaga
     3841 ccgattccca taattattac accgcctata gcagtcatct cgcttactac cacagggttt
     3901 aacaaagatt gcataatgct tgcaagcatt gttatcgtac cttgatacac aagaacgctt
     3961 atgccggaaa aaactacacc cacacccatt gtcgccgcaa aagtaaccgc tgtaatgccg
     4021 tcaagcactg cttttgaaaa taaaatttca tggtttcccg tcaatccgct ttgaattgag
     4081 cccacaatag ccatggagcc cacacagtaa accagggtgc atgcaacaaa tccaagcgct
     4141 acattgcttt ccttgttttt cattttactt tcgataaatg ctccgatttt
//
LOCUS       DAOSOW010000138         4187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_21311_length_4187_cov_3.813650, whole genome shotgun sequence.
ACCESSION   DAOSOW010000138 DAOSOW010000000
VERSION     DAOSOW010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21311_length_4187_cov_3.813650"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1230
                     /gene="mltG"
                     /locus_tag="PLD67_04615"
     CDS             214..1230
                     /gene="mltG"
                     /locus_tag="PLD67_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOT21835.1"
                     /translation="MKKIIALLCVLSIIAAAYLYLNNYIEENLEAVDLGDDSPIVVEI
                     PAGSTTNDIAEILFENNLISNIRVFKYYAEKTGSDAKLKAGTYILSKSMNADELLESL
                     IKGGSSGNTYNITVIEGLTNKDMAQSLSEQTALNYDRFIELMESPEIFNQDFEFLKDS
                     SVTNLQGYLMPDTYNIYVDSTEEDIIRVLLKQFDKFYIDEIKPRIDKTNLTFEEVINL
                     ASIVEKEALLDEERDEVAKVFLNRLSINMKLQSCATVNYANGEWKERLTLEDIEIDSP
                     YNTYIVEGLPPTPINSPGRVSIIAVLEPADVDYLYFVAKGDGSHYFSNTYDEHIDAAE
                     EYLN"
     gene            1242..1886
                     /locus_tag="PLD67_04620"
     CDS             1242..1886
                     /locus_tag="PLD67_04620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOT21836.1"
                     /translation="MNNINSSYINEYINSLIIEDSEKLNKFRDYCEKRSLPIIHKEVG
                     QFIKLVINLLNAKSIIEIGTNVGYSSIFMSKVMKDEGKVVTLERSEKFYNEALKNITD
                     FGLEKKIEVYFGDAVDILDQVEGTFDMAFIDAAKSYYRVFFDKCLKMMKPGGIILSDN
                     VLYQGMIASDELVVRRKKTLVRNLRNYLEYISHDERFVTSVLPLGDGLAVTLIK"
     gene            1967..3190
                     /locus_tag="PLD67_04625"
     CDS             1967..3190
                     /locus_tag="PLD67_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007788149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOT21837.1"
                     /translation="MLPKLLAPAGNLDKLIMAVQYGADEVYFAGKSMGMRAGSKNFTK
                     DDLKQGVEYCHKYGRKANITVNIIPHNDDLKGIEGYLKYLHKIGTDALIVADPGVVEI
                     VKQTIPDMKIHLSTQANTTNYHTANFWYKQGVNRIVLARELTFEEIETIIKNTPEDLE
                     FETFVHGAMCISYSGRCLLSSFMTGRFSNKGDCAQSCRWKYNLVEEKRPGEYYPIEET
                     GEGTFIMNSKDLCMIEHLQKFKELGIKALKIEGRNKSEYYTALTVRSYRNALDELKYP
                     ESERNTGYWKEELEKTSHRDFSYGFFFGNPYKDGQLYTHSSYIRTYDVVGIIRSYDES
                     SKIATVEQRNKFSEGDILECFGPKGKHFELKAENLTDENNNKIDSAPHPQQTVKMYIE
                     KNVEPYFILRKKLEE"
     gene            complement(3261..3962)
                     /gene="sigK"
                     /locus_tag="PLD67_04630"
     CDS             complement(3261..3962)
                     /gene="sigK"
                     /locus_tag="PLD67_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HOT21838.1"
                     /translation="MIQELLQILLPLSIFIFGYITNGSSFPHPLTKEEEACLIERANK
                     GDIEARNILIERNLRLVAHVIKKYKTVGIDQEDLISIGTIGLIKAVSTFDEKKGIRLA
                     TYAAKCIDNEVLMTIRSEKKLKKEVSLQGPLGTDKEGNNIYLIDIIKYEGEEIVGRLD
                     KEEKIKALDEILDKTLQKRERFIIEMRYGIRNKEHKTQREIAKLLGISRSYVSRIEKK
                     ALKKLHDALVNNVGN"
BASE COUNT         1446 a          629 c          778 g         1334 t
ORIGIN      
        1 aatcacatct aaattaccaa ctttggataa atcgggagtg ctaattttgt tcaatctgtc
       61 cattagtatc aaccctctct taatcaaaag aaataagcat tattatatta accagtcaaa
      121 taattattat actattaatt ttttaaatta ttgtaattat tagtaaaagg ttgtataatt
      181 taatggattt agaaattatc tactggagga attatgaaaa agataattgc actactgtgt
      241 gttttgtcaa taatagcagc ggcgtatctt tatttaaata attatattga agaaaattta
      301 gaagctgttg atttggggga tgattctccg attgttgttg aaattcccgc aggaagcaca
      361 actaatgaca ttgctgaaat tttgtttgaa aataatttaa tttcaaatat acgcgtattt
      421 aagtattatg ctgaaaagac agggtcagat gctaaattaa aggccggaac ctatattctt
      481 tcaaaaagca tgaatgctga tgaacttttg gaatccctta taaaaggagg ctcatcaggc
      541 aatacttaca atatcacagt gatagaaggt ttgactaata aagatatggc tcagtccttg
      601 tcggagcaga ccgctttaaa ttatgacaga tttattgaat tgatggaatc tccggaaatt
      661 ttcaatcaag attttgaatt cttaaaagat tcatctgtta caaatttgca gggataccta
      721 atgcctgata cttacaacat ttatgttgat tcaacggaag aagatattat tagagttctt
      781 ttaaaacaat ttgataaatt ttatattgat gaaattaaac caaggataga taaaacaaac
      841 cttacatttg aagaggttat aaatttagca agcatagttg aaaaggaagc tcttttggat
      901 gaagaaagag atgaagttgc aaaagttttt ttaaaccgtc ttagtattaa catgaagctt
      961 caatcctgtg cgaccgttaa ctatgcaaac ggagagtgga aggaaagact tacccttgag
     1021 gatattgaaa ttgattcacc atacaatact tatattgtgg aaggactgcc tccaactccc
     1081 ataaattcac ccggcagagt atcaataata gctgtattgg agcctgcaga tgttgattat
     1141 ttatactttg ttgcaaaagg agacggctct cattatttct caaacacata tgatgagcat
     1201 atagatgcgg ctgaagagta tttgaattga ggaggtatac tttgaataat attaactcat
     1261 cttatataaa tgaatatata aacagcctta ttattgaaga tagcgaaaaa ttaaataaat
     1321 tcagagatta ttgcgaaaaa agaagtctgc ctatcatcca taaggaggta ggccaattta
     1381 ttaaattagt tataaatctt ttaaatgcaa aaagtataat tgaaataggt accaatgtag
     1441 gttattcttc aatatttatg agcaaggtta tgaaagatga gggtaaggtt gtcacattgg
     1501 agagaagtga aaaattttat aatgaagcat taaaaaatat tactgatttt ggacttgaaa
     1561 aaaaaataga ggtttatttt ggagatgctg ttgatatatt ggaccaagta gaagggactt
     1621 ttgatatggc ttttatagat gcagccaaaa gctattacag ggtatttttt gacaaatgct
     1681 taaaaatgat gaagcccgga gggattattc tttcggacaa tgtattatat caaggaatga
     1741 ttgcatcgga tgaattggta gtaagaagaa agaaaacatt agtaagaaat ttaagaaatt
     1801 atttagaata tatatctcat gacgagagat ttgtaacttc ggtacttccc cttggggatg
     1861 gattggcagt tacgctgatt aaatagggat ggaacacacc gaaaactttg ttttttaaag
     1921 aatgtcgctt taattgaaat aaccagatag agaaagagga gaaagaatgt tgccaaaatt
     1981 attagcacct gccggaaatt tagacaagtt aataatggcc gtacaatacg gagccgatga
     2041 agtttatttt gcgggaaaat caatgggaat gagagcaggg agcaaaaact tcactaaaga
     2101 cgatttaaaa cagggagtgg agtattgtca taaatatgga agaaaagcaa atattaccgt
     2161 taatataatt cctcataatg atgatttaaa gggtatagaa ggatatttga aatatttgca
     2221 taaaatcggt acagatgcac tgattgtagc tgacccggga gttgtggaaa ttgtaaagca
     2281 aacaattccg gatatgaaaa ttcatttaag cacccaggca aatacaacaa actatcacac
     2341 agcaaacttc tggtataaac agggagtaaa cagaattgtg ctagccaggg agctgacatt
     2401 tgaggaaatt gaaactatta taaaaaacac accggaagat ttagaatttg agacatttgt
     2461 acatggagcc atgtgcatat cctattcagg cagatgcctc ttatcaagct ttatgaccgg
     2521 caggttttca aataagggtg actgcgccca atcctgcaga tggaaataca acttggttga
     2581 agagaaaaga cccggggagt attatcccat agaagaaacc ggtgaaggta cttttattat
     2641 gaattccaag gatttatgca tgatagagca tttgcagaaa ttcaaggagc tgggaataaa
     2701 agcgttaaaa attgaaggta gaaacaaaag tgaatactat accgctttaa ccgtccgctc
     2761 ttacagaaat gcattggatg agctgaaata tccggaaagt gaaagaaata ccggatattg
     2821 gaaggaagaa cttgaaaaaa catcgcacag ggatttttcc tatggatttt tcttcggtaa
     2881 tccctacaag gatggacagt tgtataccca ttcaagctat ataaggacat atgatgtggt
     2941 tgggataata aggtcgtatg acgagtcatc aaagattgca acagtggagc agagaaataa
     3001 attttcagaa ggcgatattt tggaatgctt tggtcctaaa ggaaaacact ttgaattaaa
     3061 agcagaaaac ttaacagatg aaaataataa taaaatagat tcagcacccc atccgcagca
     3121 aacagtaaaa atgtatattg aaaaaaatgt agagccatac tttattttga gaaaaaaact
     3181 ggaagaataa aattaaataa atgacaaggg aacgggggca ctgtcatctg aatgatgaca
     3241 gcacccccgt ccccttgtca tcaatttcca acgttgttca ccaaggcgtc atggagtttt
     3301 tttagagcct tcttttcgat tcttgataca taggagcgtg atattccaag aagtttggca
     3361 atttccctct gagttttatg ctctttgttt cttattccgt aacgcatttc aataataaat
     3421 ctttctcttt tttgtaatgt tttatccaat atttcatcca gggcttttat tttttcttct
     3481 ttatccaatc tgcctactat ttcttctcct tcatatttta taatatcgat gagataaata
     3541 ttattgcctt ccttgtctgt tcctaacggt ccttgaagag atacttcttt ttttagtttt
     3601 ttttcacttc ttatagtcat aagtacctca ttgtcgatgc atttggcagc ataggtggca
     3661 agtcttattc cttttttttc atcaaaagtt gaaacagctt taattagacc gattgttcct
     3721 atggatatta aatcctcttg gtctattcct actgtcttat atttttttat aacatgtgcc
     3781 acaagacgaa gatttctttc tataagaata tttcttgctt ctatatcgcc tttatttgct
     3841 ctttctataa ggcatgcttc ttcttccttg gttaaaggat gtggaaatga gcttccgtta
     3901 gttatgtaac caaaaataaa tattgatagc ggtaataaga tttgtagtag ctcttgtatc
     3961 aaaatcaccc ccaaagttat acattatatg caagatatta aaaaaactgt atgtacactt
     4021 taaaaacttt aaataaaagt aatatttaac agcatttctg ccattaaata ttacacttgt
     4081 ttttcttaca tttcctcatt cataggcggg acagttccat tttctcctcc tccaccgtcc
     4141 atcgggcagt tcatacgtct catcagttct cttaattgct cgcagtt
//
LOCUS       DAOSOW010000139         4178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_21363_length_4178_cov_3.037594, whole genome shotgun sequence.
ACCESSION   DAOSOW010000139 DAOSOW010000000
VERSION     DAOSOW010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21363_length_4178_cov_3.037594"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1193
                     /locus_tag="PLD67_04635"
     CDS             <1..1193
                     /locus_tag="PLD67_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21839.1"
                     /translation="NSSNEDVSVHMAFPFIEKFSEFSPDDIIITANDEIIPYDTYLGE
                     TINNSMNPFQEAENTDFNIEKIIKSITDKKYTANNFKENERGKLYRFKVSSADDKINF
                     AVSFDFNHEKTKIILNGFNRFERNENNIRVASGTREEETREIYVLGEDISFDIKGYTS
                     GNLEEETDKFEYEIITEEIDVETFLKNYAAYYLNKYADNLEEMAKGQISEIQLYNLYA
                     ESLDKYLDRNLGVCTEGDIMSETWVPRIITLVYSVNFPQNSKKEISVSYKTNGTMDRR
                     ETAEPQFTYDYILNPARNWKGFKNLNIKIITPEEAPYIVTSSIELNKEDNNVYTAYSE
                     ELPDEDFSFTLYPKEEISLSDRIEGYLNRTFGYFTPIVIGFVFIITVIVILVVVKTKK
                     RNKE"
     gene            1436..3082
                     /locus_tag="PLD67_04640"
     CDS             1436..3082
                     /locus_tag="PLD67_04640"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOT21840.1"
                     /translation="MKKSRFKKFVLVGLTCTIISTSAIFALADNFDANKAIRLQINNE
                     WVDAQNLYIENDRAMVPLDGIIEELGLKTESDLATNTVKIYDEELTIILTIGNNTAGV
                     IKVISGEIIEEANNLDVAPKIINNQIFVPIRFVAETFGLQVSWDDTLRAVIIEAASGI
                     SVERPIDFEVLDFQAIMDNDLLKNLYDNNYMTKGIYSLVDGEWMYVLAAAGEKPTGGY
                     DLTIDSITEVTKGTAYIHVTLTSPEEGSMVTQVLTYPTVFVKFNKGDIRDIQWDVTGD
                     VTEPETDEQEKVLIENLVKSFGEKLKMVSLLATEDMVKESIKEYYGEFFSQVLLEKWL
                     SDPAKALGRTTSSPWPERIDIISFEKLSDTEYEVKGNIIEMTSVELTQGGIAASRPAT
                     FIIRNIDGKWLIDDIKIGDYIDLNLLVYDNKEYGFYFTLPASWIGYSVIEDKWEGVSL
                     IDSKANQSGSIFYIRHPEWTEKTPRQDIPIMVFTKDQWESLNNGEFSVGAAPVGPSIL
                     GENTSYVFALPARYNYEFLTGFEEVEEILENDSLQAYEVN"
     gene            3306..4088
                     /locus_tag="PLD67_04645"
     CDS             3306..4088
                     /locus_tag="PLD67_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HOT21841.1"
                     /translation="MFDNQNDAVNNLLNKEGMTVKDRYLPIEGYTRVEYEEGSFAEFL
                     RNQKLKPYGEKVLYYDGRQKPAKGIYDSVFDVDIGNRDLHQCADAIMLLRAEYLYSQG
                     LYDKISFNFVSGFKAEYKKWMNGYRIDVQGNNAEYYKTAEPSNTYENFRKYMDMVFAY
                     SGTLSLEKELISVSIDNMSIGDVFIVGGSPGHAVIVVDMAVNSLGEKIFLLAQSYMPA
                     QQTQLLINPMDEDISPWYSLKGKEKLITPEWTFELDTLKSWY"
BASE COUNT         1530 a          562 c          845 g         1241 t
ORIGIN      
        1 aaaattcttc aaatgaagat gtttcagtac atatggcatt tcctttcatt gaaaaattca
       61 gcgagttttc accggatgat attattatca ctgcaaatga tgaaattatt ccctatgata
      121 cctatttagg tgaaacaatt aacaattcaa tgaatccttt tcaggaagct gaaaatactg
      181 actttaatat tgagaaaatt ataaaatcta ttacagataa aaagtatacc gctaacaatt
      241 ttaaagaaaa tgaaagaggt aagctttaca ggtttaaggt atcttctgct gatgacaaaa
      301 taaatttcgc cgtgagcttt gattttaatc atgaaaaaac caaaattatt ttaaatggtt
      361 ttaatcgctt tgaacgaaat gaaaataata tcagagttgc ttccggcaca agagaagaag
      421 agacaagaga gatctatgta ttaggtgaag atattagttt tgatattaaa ggttatacaa
      481 gcggaaatct tgaggaagaa accgataaat ttgaatatga aataataaca gaagaaatag
      541 atgtagaaac attcctgaaa aattatgcgg cttattattt gaataaatat gctgataacc
      601 ttgaggagat ggcaaaggga caaatatcag aaatccagtt gtataacctt tacgcagagt
      661 ctttggataa atacttagac aggaatttag gagtttgtac cgaaggtgat attatgtctg
      721 aaacctgggt gccacgcata attacattag tctattctgt gaattttccc cagaattcta
      781 aaaaggaaat aagtgtgagc tacaaaacga atggaacgat ggataggcgg gaaacggcag
      841 aaccccaatt tacatatgat tatattttaa atccggcaag gaactggaaa ggatttaaaa
      901 acttaaatat taaaatcata actccggagg aagctcctta tattgtaacc agcagcattg
      961 aattaaataa agaggataat aatgtttata ctgcctattc agaagaatta cccgatgaag
     1021 acttctcctt tacactttat ccaaaggaag aaatttcatt atcagacaga attgaaggat
     1081 atttaaacag aactttcggg tatttcacac ctatagtaat aggttttgta tttataatta
     1141 ctgttattgt tattcttgtt gttgttaaaa caaagaaaag aaataaagaa taaataaaag
     1201 agtttattga ccaccgggga cggttctcac cggacaaggt tcaatacaag aatcctaata
     1261 acaactaaaa aattaatatg atttaagggt ggctgatata gatgaaatcc atatcggcca
     1321 tttctataat aaatttaaaa tttctagctt gagtaagatt taaggtttga ggaacaaata
     1381 agataaattt gcgtcttatt tatatatcaa tttgtttgtt acaggaggaa aagacatgaa
     1441 aaaatcaagg tttaagaaat ttgttcttgt aggattaact tgcacaatca tatccacaag
     1501 tgccatattt gcattagcgg acaattttga tgcaaacaaa gcaataaggt tgcaaataaa
     1561 caatgaatgg gtggatgcac aaaatcttta tattgaaaat gaccgtgcaa tggttccatt
     1621 agacggtatc atagaagaat tagggttaaa gactgaaagt gatttagcaa caaatacggt
     1681 aaaaatttat gacgaagaat taacaattat tttaacaata ggtaacaata ctgccggggt
     1741 tataaaggtt ataagcggtg aaattattga ggaagctaat aacttggatg tagcacctaa
     1801 aataattaat aatcaaatat ttgttcccat acgctttgtt gctgaaacat ttggtttgca
     1861 ggtaagttgg gatgatacat taagggcggt tataatagaa gccgcatccg gtattagcgt
     1921 tgaaagacct attgatttcg aggttttgga ttttcaggca attatggata atgacttgct
     1981 gaaaaattta tatgataata attatatgac aaaaggtatt tattcattgg ttgacggaga
     2041 atggatgtat gtattggcag ctgcagggga gaaaccgact ggaggatatg atttgaccat
     2101 agacagtatt acggaagtta caaagggtac ggcatatatt catgttactt tgacttctcc
     2161 cgaagaaggc agcatggtga cccaggtgtt aacttatccc acagtttttg taaagttcaa
     2221 caagggtgac ataagagata ttcaatggga tgtaacaggt gatgttactg aaccggaaac
     2281 tgatgaacaa gaaaaagtgc tgatagaaaa tttagttaaa agttttggag aaaagctgaa
     2341 aatggtttcc cttttggcaa cggaggacat ggtaaaagaa agtataaaag aatactacgg
     2401 ggaatttttt tcacaggtac ttcttgaaaa gtggctaagt gatccggcaa aggctctcgg
     2461 acgaacaact tccagcccat ggccggaacg tatagatatt atatcgttcg aaaaattgtc
     2521 tgatactgag tatgaagtaa aaggaaatat aattgaaatg accagcgttg aattgacaca
     2581 agggggtata gctgccagta gacctgcaac atttataata agaaatatag atggaaaatg
     2641 gctaatagat gatataaaaa taggggatta tattgacctt aatttattag tatatgataa
     2701 taaagaatac gggttttatt tcaccttgcc agcaagctgg ataggataca gtgtaataga
     2761 agataaatgg gaaggtgtat cattaattga ttcaaaagca aaccaatcag gctcgatttt
     2821 ttatataagg catccggagt ggaccgaaaa aacccccaga caggatattc ccatcatggt
     2881 atttaccaag gaccagtggg aatccctcaa taacggggaa ttcagcgtgg gtgcagcacc
     2941 tgtcggtcca agcatattag gtgagaatac gagctatgtt tttgccttgc ctgcaagata
     3001 caattatgaa tttttgactg gatttgaaga agtagaggag atattggaaa atgactccct
     3061 tcaggcatat gaagtaaact aattcttttg agcatttcaa atcatgagca tatatactat
     3121 aattattata caaagagcaa actacattta tgagcatccc aaattgctgg caaaaactat
     3181 tttaataata tggagagact gtgatggtgt ccataagaag attatgcatt tatggtttat
     3241 taataggttt gttaataaca ggatacaaaa atgtcggatt ggattcgact gaagtacctt
     3301 ttaatatgtt tgataatcaa aacgatgctg taaataacct ccttaacaag gaaggaatga
     3361 ctgttaaaga cagatatttg ccaatagaag gatacacaag ggttgaatat gaagaaggta
     3421 gctttgcgga atttttgagg aatcaaaagc tcaaacccta tggtgaaaag gttttatatt
     3481 atgacggtag acaaaagccg gcaaaaggaa tttatgatag tgtatttgat gtagatatcg
     3541 gcaacagaga cctccatcag tgtgcggatg ctattatgct tttaagggca gagtatcttt
     3601 attcacaagg actatatgat aaaataagct ttaattttgt atcaggattt aaggcagaat
     3661 ataagaagtg gatgaatggt tacagaattg atgtacaggg aaacaatgca gagtattata
     3721 aaactgcaga gccttcaaat acatatgaaa atttcagaaa atatatggat atggtttttg
     3781 catattccgg aactctttct ttggaaaaag agctgataag tgtaagtatt gataatatga
     3841 gtataggcga tgtgtttata gttggcggaa gtcccggcca tgccgtaata gttgttgaca
     3901 tggcagttaa ttcattaggt gaaaaaatat ttttactggc acagtcctat atgcccgccc
     3961 agcaaactca acttcttata aatccgatgg atgaggatat tagtccatgg tattcattaa
     4021 agggaaaaga aaaattaata acacctgaat ggacttttga acttgataca cttaaaagct
     4081 ggtattagta gataagtaaa tatctttagt tttattataa gaaaattccg aataagttaa
     4141 tttctaaatc attggaaaca gaagatgtta atttttcc
//
LOCUS       DAOSOW010000140         4160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_21473_length_4160_cov_2.695006, whole genome shotgun sequence.
ACCESSION   DAOSOW010000140 DAOSOW010000000
VERSION     DAOSOW010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21473_length_4160_cov_2.695006"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..1105)
                     /gene="pfkA"
                     /locus_tag="PLD67_04650"
     CDS             complement(146..1105)
                     /gene="pfkA"
                     /locus_tag="PLD67_04650"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005366041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOT21842.1"
                     /translation="MRKLAVLTSGGDSPGMNAAIRAVVRYSIYNNISVIGIRNGFQGL
                     VEGDHTEMTLSSVADIIHRGGTILGSARSDEFMKEEGFSKALKNLKYLGIEGLVIIGG
                     NGSFKGAQKLEKAGVSTVCIPGTIDNDLPYTRYTLGFFTALTTVLESVEKIRDTSSSH
                     NRSNVIEVMGRNCGDIALYAGLAGGAEMVIIPEIEYNIDEICDKIMQGRTRGKRHSII
                     ILSEGAGDAREISELIEKKTGIITRYSILGYTQRGGAPAAYDRLIGSQMGAYAVKLLC
                     GNLRNRAVGVNGGGIFDMDLDEAVSVEKVFDKTTYELTKILSI"
     gene            complement(1124..>4160)
                     /locus_tag="PLD67_04655"
     CDS             complement(1124..>4160)
                     /locus_tag="PLD67_04655"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOT21843.1"
                     /translation="YLLEDNYDEALKTALLYKDIFGENNFFLELQDHGMEEQKAVNSS
                     LLKLSKETGIKLVATNDVHYINKDDAYFHDVLLCIQIQKTIVDEDRMKFPSDEFYLKS
                     YEEMAELFPEEVLRNTADIAERCNVELDFNTVHLPEFKVPKGYEKSEYFKKICQEGLK
                     ERYKNITEEIQQRLDYEIGVIEQMGYVDYFLIVWDFIKYARDNNIMVGPGRGSAAGSL
                     VAYSMKITNIDPLKYNLIFERFLNPERISMPDIDVDFCYERREEVIDYVKEKYGQERV
                     AQIITFGTMAARGSIRDVGRALNMPYGEVDFIAKKIPMTLGMTINTALEVNKELKDLY
                     DSDIKVNKLINLAMKVEGLPRHSSTHAAGVLISKEEVTKYVPLSRNKDVITTQFNMVE
                     LEELGLLKMDFLGLRTLTVIRDAIELIKENHNVKIDFQNCNYDDKEVYKLFANAETLG
                     IFQFESSGMRAFLSELKPSEFENLSAANALYRPGPMGQIPVYIKNKLNPDKVTYLHPK
                     LEPILNVTYGCMVYQEQVMQIARDIGGFTMGRSDSLRRAMGKKKMDVMLKERQNFIYG
                     KRAENREPEITGAVKNGVDEKTANEIYDLMVEFAKYAFPKAHSVAYAALAYETAWLKT
                     YYPVEFMAALISSIMGSAGTVSLYIRECKRLGIEILPPDINESMGKFTVENGKIRFGL
                     AAVKNVGTNVIQEIVKAREKKGKFTSFADFCSKVDPLVLNKRQIESLIKCGAFDFLNV
                     RRSQLMAIYERTIDSVLAQKKRNIEGQFSLFNSMETEVDIVDELPELPEYNEKALLSM
                     EKEMVGIYLSGHPLSEYEKILEKYATTNSSEISEIMDANEGNVLYDGSRVVMGGIIIK
                     KQNKRTKNNNMMAFITLEDLYGTVEGIIFPKIYDDCKDILYEDNIVLVEGTIDTSEEE
                     SPKLIINKVTELKKEYENDIKRSKLYIKVKDTESYKNLKKDLFYCICKHKGEDCVIIY
                     NEKDKASMVLSDKYRVNTEDEILFKDLKKLVGEKNIAII"
BASE COUNT         1072 a          936 c          637 g         1515 t
ORIGIN      
        1 actatttttg ttttgcgcat attcagcata tatttcaact ccctcgtatg gtgcttcgtt
       61 gtccctaaaa tgacaagggg acgggggtag tgtcattaag tgggacaaaa aggatcgtcc
      121 ccagtgtctc cgttttccct atacattaga ttgacagtat ctttgttaat tcatatgttg
      181 ttttatcaaa tactttttca acagaaacgg cttcatccaa atccatatca aaaattccgc
      241 ctccgttaac tcccacggct ctgtttctta aattgccgca taataatttc acggcataag
      301 ctcccatctg gcttccgatt aatctgtcgt aagctgcagg tgctccgcct ctttgcgtat
      361 aaccaagtat ggaatatctt gttattatcc ctgtcttttt ctctatcagc tctgatattt
      421 ccctcgcgtc acctgctcct tccgaaagaa ttataatgct gtgtcttttc cctctggttc
      481 ttccctgcat tattttatcg catatttcat ctatattata ttcaatttca ggaataataa
      541 ccatttcagc cccgcctgca agccctgcgt aaagtgcaat atcgccgcag tttcttccca
      601 taacctcaat aacatttgac ctgttgtgag atgatgatgt atctcttatt ttttcaaccg
      661 attccaatac agttgtgaga gctgtgaaaa atcctaaggt atacctcgta tatggtaaat
      721 cattatcgat ggtcccggga atacacacag tggaaacacc tgccttttca agcttttgtg
      781 cgcccttaaa agagccgttt cctcctatta tgaccaatcc ttctatcccc aagtacttta
      841 aattttttaa ggctttgcta aagccttctt ccttcataaa ctcatcgctt ctagcacttc
      901 caaggatagt tcctcctctg tgaataatgt ctgcaacaga ggatagagtc atttccgtat
      961 ggtctccctc aaccaaaccc tggaatccgt tgcgaattcc tattacactt atgttattat
     1021 aaatagagta tctgacaact gcccttatgg ctgcattcat gcccggtgag tcgcctccgc
     1081 ttgttaatac tgctaatttt ctcatcattg cctcctctca caactatata attgcgatat
     1141 tcttttctcc aaccagtttt tttaagtctt taaataatat ttcatcttct gtatttactc
     1201 tatatttatc tgataaaacc atacttgctt tatctttttc gttatagata ataacacagt
     1261 cctctccttt gtgtttacat atacaataaa ataaatcttt ttttagattc ttatagctct
     1321 ctgtatcctt cacctttata tacaatttac ttcgtttaat atcattttca tactcttttt
     1381 tcagttcggt cactttattt ataataagct ttggtgattc ttcttcggag gtgtctattg
     1441 ttccttcaac caatactata ttgtcttcat atagaatatc cttacagtca tcgtaaattt
     1501 tcggaaaaat tattccttcc acggttccgt acaagtcttc caatgttata aatgccatca
     1561 tattgttatt tttggttctt ttattttgtt tttttataat tattccgccc ataacaaccc
     1621 tgctgccatc gtataaaaca tttccttcat tagcatccat tatttctgat atttcactgg
     1681 aatttgttgt agcatacttt tccaatatct tttcgtattc agaaagagga tgcccgctta
     1741 aatatattcc aaccatttct ttttccatgg ataaaagagc tttttcgtta tattcaggca
     1801 gctccggcag ttcatccaca atgtcgactt ctgtttccat tgaattgaaa agggaaaact
     1861 gaccttcgat atttcttttc ttttgagcca acactgaatc gattgtcctc tcataaattg
     1921 ccataagctg tgagcgtctt acatttaaaa agtcaaaagc gccgcatttt atcaaagatt
     1981 caatttgcct tttgtttaat acaagcgggt caaccttcga gcaaaaatca gcaaagcttg
     2041 taaacttacc ctttttttct cttgccttaa ctatttcttg tattacattt gttcctacat
     2101 tttttacggc ggctaaaccg aagcgtattt tcccgttttc caccgtaaat ttccccatac
     2161 tttcattaat atccggcgga agtatttcta ttccaagtct tttgcactct ctaatgtata
     2221 gagatactgt cccggcgctt cccataatgc ttgatatgag cgctgccata aattcaacgg
     2281 gataataggt ttttagccat gcagtttcat atgcaagggc ggcataagct acggaatgtg
     2341 cctttgggaa tgcatattta gcaaattcaa ccattaaatc ataaatttca ttggctgttt
     2401 tttcatcaac accgttttta acagcacctg ttatttcagg ctccctgttt tccgctcttt
     2461 tcccgtaaat aaagttttgc ctttctttta gcataacatc catttttttc ttgcccatgg
     2521 cacgccttaa ggaatcggac cgtcccattg taaatccgcc gatatctctt gctatttgca
     2581 taacttgttc ctgatatacc atgcagccat aggttacgtt aagaatgggc tctaatttcg
     2641 gatgaagata ggtaacctta tcaggattta gcttattttt tatatatacg ggtatctgcc
     2701 ccatggggcc cggcctgtaa agagcattgg cggctgagag gttttcaaat tccgaaggct
     2761 tcagctcgct taaaaatgct ctcatgcctg aactttcaaa ctgaaaaata cccagcgttt
     2821 cagcatttgc aaaaagcttg taaacttcct tgtcatcata attgcagttt tgaaaatcta
     2881 tttttacatt gtgattttct ttgattaatt ctattgcatc acgtattaca gtaagagttc
     2941 ttaaccctag gaagtccatt tttaacagtc ccagctcttc aagctcaacc atgttgaatt
     3001 gagttgttat aacgtcttta tttcttgata gaggaacgta ctttgttact tcttccttgg
     3061 atatgagaac acctgctgca tgcgttgaag aatgtctcgg gagcccctca accttcatag
     3121 ccagattaat caacttattt actttaatat cgctgtcata taaatccttt aattctttgt
     3181 ttacttctaa ggctgtattt atggtcattc ccaatgtcat gggtattttt ttggcgatga
     3241 aatccacttc accgtacggc atgtttaacg ctcttccaac atcacggatg gagccccttg
     3301 ctgccattgt gccgaatgtt attatctggg caactctttc ctgtccgtat ttctctttta
     3361 catagtctat aacttcttca cgtcgctcat agcaaaaatc cacgtcaata tccggcatac
     3421 tgatacgctc gggatttaaa aatctttcaa aaatcaagtt gtattttaaa gggtcaatat
     3481 ttgttatttt catggaatat gcaaccaagc ttcctgctgc agagcctctt ccgggtccca
     3541 ccataatgtt gttgtccctg gcatatttga taaaatccca cactatgaga aaataatcca
     3601 catatcccat ctgctcaatt acacctattt catagtccaa tctttgctga atttcttcag
     3661 tgatattttt atatctttcc ttcaatccct cttggcatat ttttttgaaa tattctgatt
     3721 tctcataccc cttaggaact ttaaattcag gcaggtgcac agtgttaaaa tctagctcta
     3781 cattgcatct ctctgcaata tctgcggtat ttctcagtac ttcttcgggg aaaagttcag
     3841 ccatttcttc ataggatttc aggtaaaatt cgtccgacgg gaatttcatc ctatcttcat
     3901 ccactattgt tttctggatt tgaatacaaa gaagcacatc gtgaaagtaa gcatcatcct
     3961 tgtttatata atgaacgtca tttgttgcaa ccaactttat gcctgtttct ttgctgagct
     4021 tcaacaagga gctgttcacg gctttttgct cttccatgcc gtggtcctgc aattcaagga
     4081 aaaagttgtt ttctccgaat atgtccttgt acaacaaggc tgtctttaat gcttcatcgt
     4141 aattatcttc caataaatat
//
LOCUS       DAOSOW010000141         4149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_21568_length_4149_cov_3.040303, whole genome shotgun sequence.
ACCESSION   DAOSOW010000141 DAOSOW010000000
VERSION     DAOSOW010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21568_length_4149_cov_3.040303"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /gene="buk"
                     /locus_tag="PLD67_04660"
     CDS             complement(<1..832)
                     /gene="buk"
                     /locus_tag="PLD67_04660"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOT21844.1"
                     /translation="MKQLIINPGSTSTKIAVFDDVTMIFEKTIRHTSEELQPFNRVVD
                     QFQFRKEMIISSLKENNISLNELDAVVGRGGLLKPIEGGTYLVNDKLIKDLETNIKGE
                     HASNLGALLAHSIADEVNIPSFIVDPVVVDEMEDVARISGHPEFERICIWHALNQKAV
                     ARKAAIEKFGKKYDEVNFVVVHLGGGISVGAHRKGRVIDVNNALNGEGPFSPERTGSL
                     PAAQLVKMCFSGKYTEAEIYKIIAGKGGICAYLGTNSAKEVSDRANDGDDKARLIYSA
                     M"
     gene            complement(829..1737)
                     /locus_tag="PLD67_04665"
     CDS             complement(829..1737)
                     /locus_tag="PLD67_04665"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HOT21845.1"
                     /translation="MNRFDELLKMAKTTVPKKIAIAAAQDEDVLMAVKSAYLEKICIP
                     ILIGNKEKIIELSQKINFNLDDIQIIESKDDNEAARTAVSLVSSGKADILMKGLVDTA
                     IILKAVLDKEIGLRTGNILSHAAVFESDRYHKLFIITDAAMNIAPSASEKKQIIENTL
                     PLCRSLNIENPKVAVICAKEKVSPKMQATVDAEILVNMNKNGEIKGCMVEGPFGLDNA
                     ISREAAALKGVKGEVAGDADILLMPNIEAGNVMYKTLTYLADSKNAGIILGAKAPIIL
                     TSRADSDEAKLYSILLAVICSKKESI"
     gene            complement(1895..3283)
                     /locus_tag="PLD67_04670"
     CDS             complement(1895..3283)
                     /locus_tag="PLD67_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT21846.1"
                     /translation="MQKINFNSMDYQGMLEAVFLATQDAISVVDEKGEHILVNPAYTK
                     ITGIEHDDIIGKNALYDIEDGEESVHMKVLSTKKPVENTKLKLRPSGKTVVAQAAPIN
                     VNGKLKGSVAVLHDISTIKDLTDKLEEAEKKLRELTYKYKPEDIIGNSKAINQVRNLI
                     EKAAKVPATVLLRGESGTGKELFANSIHAQSSRSHKNFVRVNCAALTDSLLESELFGY
                     DEGSFTGALKGGKKGLFEEADKGTIFLDEISEISLNTQAKLLRVLQEKEIMRVGSNKN
                     IPVDVRVIAATNADLAKSVREGKFREDLFYRLSILPINIPPLRERIEDIPLLVKSFIA
                     KFNDEYGRNIVDISNDALDVLMSHEWPGNVRELENIIGRAIIYMNINERVIRTTHLPQ
                     LNSNKVQNTVKSTVLTDDDVSTLDRVLSKTESAYIESVYLKFDKNKTRTAKELGISLR
                     SLYYKMEKYGIF"
     gene            complement(3299..>4149)
                     /locus_tag="PLD67_04675"
     CDS             complement(3299..>4149)
                     /locus_tag="PLD67_04675"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOT21847.1"
                     /translation="EGVISKVMDVTVQFEGEHAKNYYRAQTVPNPSSVKITGPRSVVN
                     SADKAVATINIEGAMDDVVKTVPVRIYDDTDNEIFLSAPIGNVKVTVPIYPTKYVALE
                     PTITGIPEEGYELVDVTVKPERVRIAAEQDVLDTFKKLTLEELDITGAYNNILSTRNI
                     LNAEGLVFLDMETKPVVNAVVEKIIERDLRYSYDDIQFINKIENSDVEIKETEEEITV
                     TVRGTSTLVNSLKKEDLILTLDLSETVNGINTVNLDCISEFTFKEISLSKETVTIEVS
                     KEPLEP"
BASE COUNT         1134 a          879 c          600 g         1536 t
ORIGIN      
        1 ccattgcaga ataaatgagt cttgctttat catctccgtc atttgctctg tcgctgactt
       61 cttttgcact gtttgttccc aaatatgcac aaattccgcc cttgcctgct ataatcttat
      121 atatttcagc ttcggtatat ttgccgctaa agcacatctt aactaattgc gcagcaggta
      181 atgagcctgt tctttcgggt gaaaaaggtc cttcgccgtt tagagcattg tttacatcaa
      241 ttactcttcc ctttctatgt gctccgacag aaattccgcc tcctaaatga acaactacaa
      301 aattcacttc atcatacttt ttcccgaact tttcaattgc ggcttttctt gcaacagcct
      361 tttgatttaa tgcatgccaa atgcaaattc tttcaaactc cggatgcccg cttatccttg
      421 ccacatcctc catttcatca acaaccacag ggtctactat aaatgatgga atatttactt
      481 catcggctat ggaatgcgcc aagagggctc ccaaattgga agcatgctca ccttttatat
      541 tggtttctaa atcttttatt aacttatcat ttaccaaata agtgccgcct tcaatcggtt
      601 ttagtaaacc tcctcttcct actacagcgt ccaattcatt taatgatata ttgttttctt
      661 ttaatgagga tattatcatt tcttttctga attgaaactg gtctacaacc ctgttaaaag
      721 gctgcaattc ttcgcttgtg tgtcgtatag ttttttcaaa aatcatggta acatcatcaa
      781 atactgctat tttcgtcgat gttgaccccg ggttaataat taattgtttc atatgctctc
      841 cttttttgaa caaattactg ctaataaaat tgaataaagt ttggcttcat ctgaatcagc
      901 ccttgaagta agaatgattg gagccttagc acctaatata attcctgcat tttttgaatc
      961 agctaaatat gttaatgttt tatacattac atttcctgcc tcaatattcg gcattaataa
     1021 aatatctgca tcacctgcaa cttctccctt tactcctttt aaagctgccg cttccctgga
     1081 aattgcattg tcaagtccaa aaggtccctc taccatacag ccttttattt caccattttt
     1141 gttcatattc acaagaattt cggcatcaac ggtagcctgc attttaggtg atactttttc
     1201 ctttgcacat attacagcaa cttttgggtt ttcaatattc aatgacctgc ataagggaag
     1261 ggtattttcg attatttgtt ttttttcaga tgcagaaggt gcaatattca ttgccgcatc
     1321 agttattatg aacagtttat ggtatctgtc cgattcaaaa actgctgcat gacttaaaat
     1381 atttcctgtc ctaagtccga tttccttatc gagaactgct ttaagaataa ttgcagtatc
     1441 gactaatcct ttcatcagga tatctgcctt gccgctgctg accaaggata ctgccgtcct
     1501 agcagcttca ttgtcgtctt ttgactcgat aatttgaata tcatcgagat taaagtttat
     1561 tttttgtgat agttcaatta ttttttcttt attgcctatc aaaattggaa tgcagatttt
     1621 ttcaagataa gctgatttta cagccattaa cacatcttca tcctgtgcgg ctgcaattgc
     1681 tatttttttt ggcacagtgg tttttgccat ttttaataac tcatcaaatc tattcatata
     1741 caaacacctc aaaatataac ttgcaacaat tatgccaaaa aaacaaaaaa atcgaccatt
     1801 gaaatttcaa cgatagattt ttttattaaa tcagcttgaa cttttttgca tgcaattttt
     1861 ttcaagaaag ttgcattttg caaaaaattg caaattaaaa aatgccgtat ttctccattt
     1921 tataatataa ggaccttaaa gatataccca gctctttggc agttcgggtt ttattcttgt
     1981 caaatttcaa atatacactt tcaatatatg cagattctgt tttactaagt accctatcca
     2041 aagttgaaac atcatcatca gttaatactg tactcttaac cgtattttga actttatttg
     2101 aattcagctg gggtaaatgt gttgtcctga taaccctttc atttatattc atatatatta
     2161 tcgcccggcc gataatattt tccaattctc ttacgttgcc cggccattca tgagacatca
     2221 aaacatctaa tgcatcattt gatatatcaa caatatttct tccgtattca tcattaaact
     2281 ttgcaataaa actttttacc aataagggga tatcttcaat cctttctcta agaggaggta
     2341 tatttatcgg cagaatgctg agtctgtaaa ataagtcctc tctaaatttt ccttccctca
     2401 cggattttgc caaatccgca ttagttgctg caattaccct tacatctaca ggaatattct
     2461 tattgcttcc gacacgcatt atttcttttt cctgcagaac tcttaaaagt tttgcctgag
     2521 tatttaaact gatttcagaa atctcatcaa ggaatatggt gcctttgtct gcctcttcga
     2581 aaagaccctt tttgcctccc tttaatgctc cggtaaatga gccttcatca tacccgaaaa
     2641 gctcgctttc aagaagtgaa tcagtcagtg cggcacaatt tacccttaca aaatttttgt
     2701 gtgagcggga gctttgagca tggattgaat tagcgaacaa ttccttgccc gtaccgcttt
     2761 cgcctcttaa gagaacagtt gccggaacct tagcagcctt ttctatcaga ttccttactt
     2821 gatttattgc ttttgagttc ccgataatat cttcaggttt atatttataa gttagttctc
     2881 ttaacttttt ttcagcttcc tctaatttat cggtcaagtc ctttatagta gaaatatcat
     2941 gaagaacggc aacacttccc ttcaatttgc catttacatt aattggtgct gcttgagcta
     3001 caacagtctt gccgcttggt cttaatttta attttgtatt ttctacgggt ttctttgttg
     3061 aaagcacttt catatgcacg ctttcttctc cgtcttcaat atcatagagt gcattcttgc
     3121 ctattatatc gtcatgctca attccggtta tttttgtata agccggatta actaagatat
     3181 gctcgccttt ttcgtctaca acacttattg catcctgtgt tgcaagaaaa accgcctcta
     3241 acattccttg gtaatccatg ctgttaaaat taattttttg catctttaat acctcttcct
     3301 aaggctctaa aggctctttt gaaacttcta ttgttacggt ttctttgctt aatgaaattt
     3361 ctttgaaagt gaattcagaa atgcagtcta aattaacagt gttaatacca ttaactgttt
     3421 ctgataaatc cagggttaaa attaaatctt cttttttcag tgaattaact aaggttgaag
     3481 ttccccgcac agtaaccgtt atttcttctt ctgtttcctt tatctctaca tcactgtttt
     3541 caattttatt tataaactga atatcatcat agctgtatct tagatctctt tcaataattt
     3601 tttctaccac ggcatttaca accggtttag tttccatatc caaaaatacc aaaccttctg
     3661 cattcaaaat gtttctggtt gaaagtatat tgttgtatgc accggtaata tccagctctt
     3721 ctaaggtaag tttcttaaat gtatctaata catcctgctc tgcggctatc ctcactcttt
     3781 caggtttaac ggttacatcg accaattcat aaccttcttc cggtattcct gtaattgttg
     3841 gttcaagagc tacatattta gtaggatata tcggaactgt aacctttaca tttcctatcg
     3901 gagccgataa gaatatttca ttgtcagtat catcatatat ccttaccgga acagtcttaa
     3961 ccacatcatc catggcacct tcgatattta ttgtagcaac tgctttatca gctgaattta
     4021 caacacttct tggtcctgtt atctttactg atgacggatt tggaactgtt tgtgctctgt
     4081 aatagttctt agcatgttct ccttcaaatt gaaccgtaac atccataacc ttagaaatta
     4141 ctccttcta
//
LOCUS       DAOSOW010000142         4141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_21624_length_4141_cov_3.364660, whole genome shotgun sequence.
ACCESSION   DAOSOW010000142 DAOSOW010000000
VERSION     DAOSOW010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21624_length_4141_cov_3.364660"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLD67_04680"
     CDS             complement(<1..312)
                     /locus_tag="PLD67_04680"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HOT21848.1"
                     /translation="MVVIAKVIKKASDFVDKIGCGLLILTMAGMVFFSSIQIIARVFF
                     TALAWSEELTRYLLVWSTFIGAGCVYKRGGHINVSFVQDKFKGKANKYAKILVHLICI
                     AF"
     gene            complement(336..1337)
                     /locus_tag="PLD67_04685"
     CDS             complement(336..1337)
                     /locus_tag="PLD67_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008869658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOT21849.1"
                     /translation="MKKLNFIALALIVVMVLGTACGQTVDNPPTSAEPTVIKLGMTIN
                     EQDSFYVCATKFSELVEERTNGAYDIQLFPNSSLGDERTMLESMQMGTLDSGIITSGP
                     FVNFVPEFGVFDLPFLFPDNASAYKVLDGEIGKEILSKLEDHDLKGLAYAERGFRNLT
                     NSVRPVRTAEDIKDLKIRVMENEVYLSAFKAMDVNAVPMAWTEALTALQQGTIQGQEN
                     PVNVIHAYKMWDSQKHVTMTRHAYAAAVITFSEEVFNSLPADVQEIFVQAAQEAAEYE
                     RAWVAENEEAQIADLKANGMELIEEPDLESFRQAVQTVYDQYPQYSEYLTRIKEAIK"
     gene            complement(1378..3039)
                     /gene="ilvD"
                     /locus_tag="PLD67_04690"
     CDS             complement(1378..3039)
                     /gene="ilvD"
                     /locus_tag="PLD67_04690"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOT21850.1"
                     /translation="MRSDQMKTGVERIAHRALFNALGLTEKEIRQPMVGIVNAQNDII
                     PGHLHLDDIVEAVKAGVMMAGGTPFVFPAIGVCDGIAMGHEGMKYSLISREHIADSVE
                     IMAMAHPFDALVFVPNCDKIVPGMIMAALRLNIPSIFVSGGPMMPGYYDGKKLTVSNS
                     FEAVGAAGAKIMSDERIKEIELYSCPGCGSCAGMFTANSMNCMTEVIGLGLPGNGTIP
                     AVHAARIRLAKEAGMKVMELLEKDIRPRDIVTLDSLHNALTADMALGCSTNTVLHLPA
                     IAHEAGLKIDLNEINNISKATPHLVKLSPAGEDCLVDLWQAGGIPSVLKELDNHGLLN
                     TDCITVTANTVGENIKDAYIKDNSVIRSFDDPYSKDGGLAILWGNIAPDGAVVKKGAV
                     LEEMMVHRGPAKVYNSEEECMTAIMGGKVVSGDVVVVRYEGPKGGPGMREMLTPTSAI
                     AGMGLDSSVALITDGRFSGASRGASIGHISPEAAAGGLIGIIEDGDIISIDIPNNKLE
                     LFVDENIIAERRLKFVPLEKPVPNGYLKRYRKMVTSANTGGVFED"
     misc_binding    complement(3336..3581)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3707..4099)
                     /locus_tag="PLD67_04695"
     CDS             complement(3707..4099)
                     /locus_tag="PLD67_04695"
                     /inference="COORDINATES: protein motif:HMM:NF028352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
                     /protein_id="HOT21851.1"
                     /translation="MKKISQKIDENIIRGIDTLIYTSRSVILGNDNTPNLDIGNKISL
                     YLAKIVKQLKEAPAFIVAKGGITSSDIATEGLNVEEAIVCGQIANGVPVWKCGRESKF
                     YGLVYVVFPGNVGDEDELTNVIKRLHKK"
BASE COUNT         1150 a          944 c          679 g         1368 t
ORIGIN      
        1 aaatgcaatg caaatcaggt gtacaagtat ctttgcatat ttatttgcct tacccttaaa
       61 tttatcctga acgaaggaca cattaatgtg ccctcctctt ttataaacac atcctgctcc
      121 tatgaaggtt gaccagacaa gcaaatatct tgttaactct tcactccaag caagggcagt
      181 gaaaaatacc ctggctatta tttgaatact tgaaaagaat accatccctg ccatagtcag
      241 tattaagaga ccgcagccta tcttatcaac aaagtcgctt gcttttttta taaccttagc
      301 aatgacaacc acctcctata taattactga cttaattact taattgcttc tttaattctt
      361 gtcaggtatt cgctgtattg cggatattga tcgtataccg tctgtaccgc ttgtctgaag
      421 ctttccaaat ccggttcttc aataagctcc atgccgtttg ctttaaggtc tgcaatttga
      481 gcttcttcat tttctgcaac ccatgctctt tcgtattcag ccgcttcctg ggctgcttgt
      541 acaaatatct cctgtacatc agccggaagg ctgttaaata cttcttcgct gaatgttata
      601 acagctgctg catatgcatg tcttgtcata gtgacatgtt tttgtgaatc ccacattttg
      661 tatgcatgaa taacattaac cgggttttcc tgtccttgta ttgtaccttg ttgcaaagcg
      721 gtaagtgctt cagtccatgc cataggcacg gcatttacat ccattgcctt aaatgcagat
      781 aaataaactt cattttccat cacacgtatt tttaaatcct taatatcttc agcagtgcgt
      841 acaggtcgca cactgttagt caaattacgg aaacctcttt ccgcgtaagc aagtccctta
      901 aggtcgtgat cttcaagctt agagagaatt tcttttccta tttcaccgtc aagtacctta
      961 taagcagaag cattgtcagg gaataagaag ggaaggtcaa aaacaccgaa ttcaggtaca
     1021 aaatttacaa acggtccact tgtaatgata ccggaatcaa gggttcccat ctgcatactt
     1081 tcaagcattg ttctttcatc gcccaatgat gaattgggga acaactgaat gtcatatgca
     1141 ccgttggttc tttcctctac aagttctgaa aattttgtgg cacatacgta aaagctgtct
     1201 tgttcattga ttgtcatacc taatttaata accgtcggtt cagccgatgt tggtggatta
     1261 tcaacagttt gtccgcatgc tgtacctaat accataacaa ctattaatgc taatgctata
     1321 aaatttaatt ttttcatttt tttctcctta tatattcata atttttttat taatctctta
     1381 atcttcaaat actcctccgg tatttgccga agttaccatc ttacggtatc tttttaaata
     1441 tccgtttggt accggttttt ccaaaggtac aaactttaag cgtctttctg ctattatgtt
     1501 ttcatcaaca aaaagctcaa gcttattatt aggaatgtcg atgctaatta tatctccatc
     1561 ctcaatgata cctatcaatc ctcctgctgc agcttcagga gatatgtgac caatagaagc
     1621 tcctcttgat gctcccgaaa atctgccgtc tgttataagt gcaacgcttg agtcaagtcc
     1681 cattcctgca attgcagatg ttggtgtaag catttcacgc atgcccggtc cacctttagg
     1741 tccttcataa cgaactacga ctacatcacc tgaaacaact ttaccgccca ttatggctgt
     1801 catgcattct tcttcactgt tgtaaacctt ggcaggaccc ctgtgaacca tcatttcttc
     1861 taaaacggct cccttcttta caactgctcc gtccggtgct atgtttcccc ataatatggc
     1921 taatccgcca tcctttgagt atggatcatc aaagcttctt ataactgaat tatcctttat
     1981 ataagcatcc ttgatgtttt cgcctacggt atttgccgta acagtaatac aatccgtatt
     2041 cagcagtcca tgattgtcga gttccttcag tacagacggg attcctcctg cctgccataa
     2101 atcaaccaga caatcttccc ctgcaggact taattttacc agatgaggag ttgccttgct
     2161 tatattgttt atttcgttaa ggtcgatttt tagtcctgct tcatgtgcta ttgcaggcaa
     2221 gtgcagtaca gtatttgttg agcatcccaa agccatatct gctgttaaag cattatgaag
     2281 ggaatcaagg gtaacaatat cgcgaggacg gatatctttt tccaaaagct ccattacctt
     2341 catgcctgct tcttttgcaa ggcgtatacg ggctgcatga acagcgggaa tagtaccgtt
     2401 tccgggcaag ccaagtccga taacctctgt catgcaattc atggaattgg cagtaaacat
     2461 acctgcacat gagccgcaac cgggacacga ataaagctct atttccttta tccgctcatc
     2521 gctcattatt tttgcaccgg ctgctccaac agcttcaaat gagtttgaga cagttagttt
     2581 cttgccatca taataaccgg gcatcatggg accgccgctt acaaaaatac ttggaatatt
     2641 aagccttaat gctgccataa tcataccggg aactatttta tcgcagttcg gaacaaaaac
     2701 aagtgcatca aagggatgag ccattgccat tatttcaact gaatccgcaa tatgttcacg
     2761 gcttataaga gagtatttca taccttcgtg acccattgca ataccgtcgc atactcctat
     2821 tgcaggaaat acaaagggtg ttccgcctgc catcatcact ccagccttta cagcctcaac
     2881 aatatcatcc aagtgcaaat gtcccggaat tatatcattc tgagcattta cgatgccaac
     2941 cataggttgt ctgatttctt tttctgtgag ccccagagcg ttgaataagg ctctgtgagc
     3001 aatcctttcc acgcctgttt tcatttgatc acttctcata aatttcctcc aaataaaaat
     3061 ccattttctt acttacttta aaattgatta ttctgccccc tgttttacct cctcttattt
     3121 tttgattttt gtttttatta accggaatga agttaatctc ccgaagggtt aggtctattc
     3181 gcaagcttca tcacaatttt atttgctcgc tgacgctcac ataaaattgg attacgctgc
     3241 ataagaccta ccatcaattt cttcctccct ttgggatgaa gaaattgggt taggtctata
     3301 aaaaaacctc tcgtccctat atacatatag atgtatatag ggacgagagg tctttcgcgg
     3361 taccacccta attcacgata aaaaaatatc gcctcattca agtcgaaaaa acaaaaaagt
     3421 tttttgaact ctattcttgt aacgcagaaa tacgtcctcg tctacttaat tcgactcgga
     3481 agctcaggag tgatcattat atctcataac accgatttac accaaccatc ggctctctga
     3541 agttattttc cggatatttt tgtctccgtc aaagctttaa tcaatttttt catattatat
     3601 acacaggata ttgttttgtc aaccattttt taaaaagaaa aataccggtc tttcatcgtg
     3661 gtagggaaca gaatgtctgg tacttatatc atgccggggt aaatttttat tttttatgaa
     3721 gtctcttaat gacatttgtt aattcatctt catctccaac attgcctgga aagactacat
     3781 aaaccaatcc gtaaaattta ctctccctgc cacatttcca cacgggtacg ccattagcta
     3841 tttgtccgca aactatcgct tcctcaacat tcaacccttc agtcgcaata tcacttgatg
     3901 tgattccgcc ctttgcaaca ataaaagccg gggcttcttt taactgtttt acaatctttg
     3961 caagataaag agatatttta ttaccaatat ctaagttagg ggtgttatca tttccaagta
     4021 ttacagaacg tgaagtgtaa atcaaggtat caattcccct aataatattt tcatcaattt
     4081 tttgagaaat ttttttaatt atattacttg atgctttgat gcaccaaaaa tattataaca
     4141 c
//
LOCUS       DAOSOW010000143         4103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_21877_length_4103_cov_3.913538, whole genome shotgun sequence.
ACCESSION   DAOSOW010000143 DAOSOW010000000
VERSION     DAOSOW010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21877_length_4103_cov_3.913538"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..770
                     /locus_tag="PLD67_04700"
     CDS             147..770
                     /locus_tag="PLD67_04700"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOT21852.1"
                     /translation="MMTKYKSDNVHNRTMTLTGHSEMEIAPDIVIIRLGVQTTGEDLS
                     QIQADNARISQSIIQTLQRMGITDIKTSQYSIDRVYDFEDGRQIDRGFSVRNILQIRT
                     TNLEAAGSIIDAAVNAGANVVDLITFDISNREYYYQQVLNMAILNAIQKANSITMNLG
                     YSTPALVVNIVENAAIPFLPVQREFAATPIIPGTIRIEANVTVDFVF"
     gene            complement(767..1342)
                     /locus_tag="PLD67_04705"
     CDS             complement(767..1342)
                     /locus_tag="PLD67_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HOT21853.1"
                     /translation="MDKVTLWTRQDIRSLDDLERKGVIRITRKHLEEKFDVISDHIIF
                     LYNWFVNEAEKRVPRPEGVEFFIWCSISEEYMLRPTLNEIVYVLEVDKSEVVYFDGMK
                     WDYVLNHHYVPKDEKDAEEYLKDLERKGFKNSYSFINEKTAHFYPQERKRVMDSWARV
                     FEIDEWDIFRIQANIWEIRPEMIKDILRADS"
     gene            complement(1361..2974)
                     /locus_tag="PLD67_04710"
     CDS             complement(1361..2974)
                     /locus_tag="PLD67_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21854.1"
                     /translation="MNEFLSLKILDSFKRIFEGFCIDYKLLRIILKTKLILDSRRAPV
                     INTNQKKKDEEKVSLLKGYFMHLLFGAFLAFFIYFIKDEMIRMMYFSGAFFFMTTMYL
                     ISDFSFVLLDTKDKNILLTKPVNSRTVSLAKVLHILIYMFRLNTFIAGPSLIALVMSN
                     DLFVIPIFLVEILLMDLLIFLVTAFIYLLVLRFFDGELLRNIINFIQILLTILVTVGY
                     QIAIRMIDFTSLRNMEYTFNSRHFLNPILWFGAPFEIFYHGGESYLYVFTALIIIVPL
                     VSFIVYLSLSKKMESLLLKLEGEGKERPSIHWIEKIVGFIINPNEISKQSFNFANAMF
                     RSDRSLKLKIYPALSSGILLPILMIFNMRMGGREFKPSGYEYLYLYFNLLIFSSVFYL
                     VKYSSAWKGSYVFISSGFKDYKNLYRGLMRSMAYRFFIPLIILNGVVFTLIFKFRFVE
                     DIVILFLAGIAILPLIGKIYLEIPFSKPIEESNQRDSFDKFLLSFAIVAIFAIVHFYS
                     NIIPMGKWIYLSVLAVGIPISWKFATPGE"
     gene            complement(2986..3756)
                     /locus_tag="PLD67_04715"
     CDS             complement(2986..3756)
                     /locus_tag="PLD67_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21855.1"
                     /translation="MSEVLRLEHVSKSFGSKDVLKDINFSVNSGEIIGYIGSNGAGKT
                     TTIKIILGLIDDYQGDVFIFGEKVKGQFEYKKRIGYVPEVSDMYDNLTAYEYLSFIGM
                     LYGIGEDKAVKKGKEMMSVFGIDDAINGRIHTFSKGMRQKLSIITGMIHNPDILFLDE
                     PLGGIDANSVLVFKEVLKNLKKEGKTIFYSSHILEVVEKLSDRILLINEGEIVIDGTV
                     ADVMSMQSDSSLESIFNYVTGFHDYEMLAKKYTDVFMS"
     gene            complement(4004..4093)
                     /locus_tag="PLD67_04720"
     CDS             complement(4004..4093)
                     /locus_tag="PLD67_04720"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21856.1"
                     /translation="MKAKLENGVLSIIVPKENKPNKSVKIDIE"
BASE COUNT         1409 a          779 c          560 g         1355 t
ORIGIN      
        1 aaccacctga cgtattgtct ccaaatgtcc ggcaagaacc gtcccctatg gaccagcaag
       61 aaccgtcccc tatggaccat tgacacataa tttaataaag ttcatattat aatataattc
      121 gaaaattttt aaataaaagg gggattatga tgacaaaata taaatcggat aatgtacata
      181 acagaaccat gacattgaca ggtcatagcg agatggaaat agcaccggac atagttataa
      241 tacgtttggg ggttcaaact acgggagaag acttatctca aatacaggca gacaacgcaa
      301 gaataagtca atctattatt cagacacttc aacgaatggg aattaccgac ataaaaactt
      361 ctcaatattc aattgacaga gtatatgatt ttgaagatgg aaggcaaatt gacagaggtt
      421 tttcggttag aaatatcttg caaataagaa caaccaattt agaagctgcc ggcagtataa
      481 ttgatgctgc ggtaaacgca ggtgccaatg ttgttgattt gataactttt gatatatcaa
      541 acagggaata ctattaccag caggttttga atatggctat attaaatgca attcagaaag
      601 ctaattcaat cacaatgaat cttgggtatt ctacacctgc tcttgttgtt aatattgttg
      661 aaaatgcagc aatacctttt cttcccgttc aaagagaatt tgctgctact cccataattc
      721 cggggacaat tagaattgag gctaacgtaa cagtagattt tgtattttaa gaatctgctc
      781 ttaatatgtc ttttatcatt tcggggcgaa tttcccatat atttgcttga atcctgaaaa
      841 tatcccattc atcaatttca aacactctag cccagctatc cattactctt ttcctttcct
      901 gcggataaaa atgggctgtt ttttcgttaa taaatgaata agaattctta aaaccttttc
      961 tttctaagtc ttttaaatat tcttcagcat ccttttcatc ctttggaaca tagtgatgat
     1021 ttagaacata atcccacttc atgccgtcaa aatatactac ctcagattta tctacttcca
     1081 aaacatatac tatttcattt aatgtaggtc ttagcatata ctcctcgcta atggagcacc
     1141 agatgaaaaa ctccacgccc tccggtcttg gcactctttt ctctgcctca ttgacaaacc
     1201 aattatataa aaatataata tggtcggaaa taacatcgaa tttctcctct aaatgcttcc
     1261 ttgttatcct gattacaccc tttctttcca aatcatccag acttcttata tcctgcctgg
     1321 tccataaagt tactttatcc ataaatgctc ccaataatat ttattctccc ggtgttgcaa
     1381 atttccaact tattggaatc ccgactgcca gtaccgataa atagatccac tttcccatag
     1441 gaataatatt tgagtaaaaa tgcacaatag caaaaattgc aactatagca aatgaaagca
     1501 gaaatttatc aaaagaatcc ctttgatttg attcctcaat aggtttagaa aagggaatct
     1561 ctaaatatat tttaccgata agaggcaaaa ttgcaatacc tgccaaaaat aaaattacta
     1621 tatcctctac aaatctaaat ttaaaaatta atgtaaatac tactccattt aaaataatta
     1681 agggtataaa aaacctatag gccattgagc gcattaatcc tctgtataaa tttttataat
     1741 ctttaaatcc cgatgaaata aatacatatg aacctttcca tgcagacgaa tatttcacta
     1801 aataaaacac ggatgagaat ataagcaaat taaaatacaa ataaagatat tcataaccgg
     1861 aaggcttaaa ttcccttcct cccatcctca tattaaatat cattaatatg ggaagaagta
     1921 ttccggaaga taaggcagga taaattttca gctttaagct tctgtcactt ctaaacatgg
     1981 cattggcaaa attaaatgat tgctttgaaa tttcattagg gtttattatg aatcctacta
     2041 ttttttcaat ccaatgtatt gaaggtcttt ccttaccttc cccttctaac ttaagaagca
     2101 ggctctccat ttttttactg aggcttaaat aaactataaa agaaacaagg ggaacaataa
     2161 ttattaaagc cgtaaaaaca tataagtatg attctcctcc gtggtagaat atttcaaagg
     2221 gtgctccgaa ccataaaata ggattcagaa aatgtcttga attaaaagta tactccatat
     2281 ttcgaagtga agtaaaatct atcattctta tggcaatttg ataacctacg gtaaccaaga
     2341 ttgtcaaaag aatttgaata aaattgatta tatttcttaa aagttctccg tcgaaaaatc
     2401 ttaaaaccaa taaatatata aatgcagtca ctaaaaatat cagtaaatcc ataagcaata
     2461 tttccaccag aaatatcgga ataacaaata aatcgttgct cattaccaat gcgataagag
     2521 acggacctgc aataaaggta tttaacctga acatgtatat aagtatatgc aatacttttg
     2581 ctaaagacac ggtccttgag ttcaccggct ttgtcaacaa tatattttta tcctttgtat
     2641 ccaataagac aaaggaaaaa tcagaaatca aatacatggt agtcataaag aaaaatgcac
     2701 cggaaaaata catcatgcgt atcatttcat cttttataaa ataaataaag aatgccaaaa
     2761 atgccccaaa aagcagatgc atgaaatatc ccttcaaaag agaaaccttt tcttcatctt
     2821 tcttcttttg gtttgtattt atcaccggtg cccgccttga atccaatatt aatttggttt
     2881 tcaatataat tcgaagcaat ttataatcta tgcaaaagcc ttcaaatatt cttttgaaag
     2941 aatcaagtat ttttaaagat aaaaattcat tcatatcaga ccccgtcagc tcataaatac
     3001 atccgtatat ttttttgcaa gcatttcata atcatgaaat ccggtaacat aattaaatat
     3061 tgactctaat gatgaatccg actgcatgct cattacatct gccactgttc cgtctataac
     3121 tatttcccct tcatttataa ggagtattct gtcgcttagt ttctcaacaa cttccaaaat
     3181 gtgagaagaa taaaaaattg ttttaccttc tttttttaaa tttttcaata cttctttaaa
     3241 aaccaataca ctgttggcat ctattcctcc caagggttca tccaaaaata aaatatcagg
     3301 attatgaatc attccggtga taattgacag cttttgtctc attcctttgg aaaatgtatg
     3361 aattctgccg ttaatggcat cgtctatacc aaagacactc atcatttctt ttcctttttt
     3421 tacagcctta tcttccccta taccgtataa cattcctata aagctcaaat attcatatgc
     3481 ggttaaatta tcatacatat cagatacttc aggtacatag cctattcttt ttttatattc
     3541 aaactgtcct tttacttttt ctccaaaaat aaaaacatcc ccttgataat cgtctataag
     3601 acctaaaatt attttgattg ttgtagtctt gccggcaccg ttagaaccta tatatccgat
     3661 aatttcaccg ctattcacac tgaaatttat gtcttttaaa acatccttgc ttccgaaact
     3721 tttacttaca tgctctaatc ttaaaacttc agacataatt actccttttt ttgtttttta
     3781 tattgacgaa atactgcatg attttgttca attttattaa ttgtttacaa tccggttgtc
     3841 atcctgagcg tatgcgaagg atctcatgag attcttcact gcgttcagaa tgacaccctg
     3901 cgggttattc atagcaatta cagctaagaa ccactactaa taataacata ctttttcaat
     3961 aaaaaaagca tgtggaaatt atgaccacat gctttaagtt tgattattca atatcaattt
     4021 ttactgattt gtttggtttg ttttccttag gaacaataat gctcaatacc ccgttttcaa
     4081 gttttgcttt aatgccggta gac
//
LOCUS       DAOSOW010000144         4085 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_22003_length_4085_cov_2.777171, whole genome shotgun sequence.
ACCESSION   DAOSOW010000144 DAOSOW010000000
VERSION     DAOSOW010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22003_length_4085_cov_2.777171"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLD67_04725"
     CDS             <1..371
                     /locus_tag="PLD67_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21857.1"
                     /translation="MITHDLGIVAEICDEVAVMYAGRIVEKGTLTDVFNKTKHPYTEG
                     LFNSLPNVNNRKAKLQPIPGLMPDPTDLPVGCSFSPRCPYANDNCRENKPIPRWISDT
                     HMVECSAYDDPTFHIARGEK"
     gene            371..1339
                     /locus_tag="PLD67_04730"
     CDS             371..1339
                     /locus_tag="PLD67_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21858.1"
                     /translation="MSKNLIEVKNLKKIFKTSHGMLHAVDGVSFTIEEGKTLGVVGES
                     GCGKSTTGRCILRLIEPSSGEVYFDGHDVLSFNNKELQKARKEMQIIFQDPFSSLDPR
                     KTINQIISEPIIIHNLIPNKNDRENRVLELMETVGLAERLINTYPHELDGGRRQRIGI
                     ARALAVNPKFIVCDEPVSALDVSIQAQILNLLKELQEKLGLTYMFITHDLSVVNHFSD
                     HIAVMYMGMIVEKAPAEELFTQPLHPYTQALLSAIPVPDLSERKNRIILKGEVTSPIA
                     LPEECRFVNRCLYACDSCKDHIPVLREIYPDHYVACDFPLERKGVE"
     gene            1339..2502
                     /locus_tag="PLD67_04735"
     CDS             1339..2502
                     /locus_tag="PLD67_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOT21859.1"
                     /translation="MLVLKNGTLMTMCSKTFKGDIAVKEGKIAAVDYGLPTENAQVRD
                     VSGCYIMPGIIDAHSHIGLQESGTRETDHNEKANPISPQMRAIDAINPQDIAFSESRE
                     VGITTCATGPGSINLIGGTFAAVKTYGNTVENMLLKHPVAMKAALGENPKFRYTEMGI
                     SPKSRLASAAIIRAALAAAVEYDRKAELYDSDYLQMPDRNLGLEALLPVIRGDLPLKI
                     HVHRADDIATAIRIAEEFNIHFTLDHCSEGFMITDRLLSALSKRCDGIIIGPLMNYKR
                     KLECSKKQGVKLPKLLHDAGIEFAICTDFPEMIPVCMMPQIALSVTEGLPEDTALKAV
                     TITAARILGISDRVGSLEVGKDADIAVFSGHPLDYRSVCLMTLINGKIVYERK"
     gene            2511..3566
                     /locus_tag="PLD67_04740"
     CDS             2511..3566
                     /locus_tag="PLD67_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006513943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HOT21860.1"
                     /translation="MSKNAIKFTYLSQEELIEAGAFNLKMAIEALHQGLVKFKDEQIL
                     FPEKIVQIFNEETQERINCLPATLLDEKICGVKWVSCFPPNPIRFGTQNLSAIIVLSN
                     IINGYPVCVMDGTLCSNMRVAAMGATAADKLAREDAESIGFIGAGEQAKMHLIGMKAV
                     RPGLKVCRVASKYAEEEHGFISGLQRIIPDMEFVACDTVLEKAIRDSDIIVTATSAQA
                     PLLKADWIKKGAFYSHVGGWEDEYKVVQMADKIVCDDWNTVKHRTQTVSRCYKDGIIN
                     DSNIYANIIDIIDGTKMGRENDDEFIYFNAVGLAYADISIAYSMYRHAVNQGFGRELA
                     LQETMIFDKNLAGKIVF"
     gene            3934..>4085
                     /locus_tag="PLD67_04745"
     CDS             3934..>4085
                     /locus_tag="PLD67_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT21861.1"
                     /translation="MLLLEVNNIKKYYADRLILDIKDFKAYYGEKIGIVGANGAGKTT
                     LLDIIAG"
BASE COUNT         1276 a          720 c          928 g         1161 t
ORIGIN      
        1 taatgataac tcatgattta ggtattgtag cagaaatttg tgatgaggtt gcggtcatgt
       61 atgccggcag gattgttgaa aagggtaccc tcacggatgt atttaacaag actaagcatc
      121 cttatacaga aggtctgttc aattctttgc ctaatgtaaa caatcgcaaa gcaaaactac
      181 aacctatacc cggactcatg ccggatccta ctgatctgcc tgtgggatgc tcgttttcac
      241 ccagatgccc atatgcaaat gataactgcc gtgagaataa accaattccc agatggatca
      301 gcgatacaca tatggtagaa tgctcagctt atgatgatcc gacattccat attgcgaggg
      361 gggaaaagta gtgtcaaaaa acctaattga agtaaagaat ctgaaaaaaa tttttaaaac
      421 ttcccatggc atgcttcatg cggtggatgg tgtaagtttc actattgaag aaggcaaaac
      481 ccttggtgta gttggtgagt ccgggtgcgg aaaatccact accggcagat gtatactccg
      541 actaattgag ccctcttccg gtgaagtata ttttgatggg catgatgtac ttagttttaa
      601 taataaggag ctgcaaaagg caagaaagga aatgcagata atttttcagg atccattttc
      661 ttctctggat cctcgaaaga caataaacca gattatcagt gagcctatta taatacataa
      721 tcttattccc aataagaatg acagagaaaa tcgtgttctg gaacttatgg agacagttgg
      781 ccttgctgaa agactcatta acacttaccc ccacgagttg gatggtggac gaagacaacg
      841 tattggtata gcccgtgcac ttgcagtaaa tccaaaattc attgtttgtg atgaaccggt
      901 gtctgcattg gatgtttcca tacaggcaca gatacttaat ctgctgaaag agttacagga
      961 aaagctaggt cttacatata tgtttattac acatgatctt tccgtggtta accatttctc
     1021 agaccatatt gcagttatgt atatgggtat gattgttgag aaggcacctg cagaagaatt
     1081 gtttacacaa cctctgcatc catatacaca ggcattgctt tctgcgatac ccgtgccgga
     1141 tctgagcgaa cgtaagaatc ggataattct taagggggaa gttacatctc caatcgccct
     1201 accggaagag tgcagattcg taaatcgttg tctatatgca tgtgattctt gtaaggatca
     1261 tatacctgtg ctccgggaga tttaccctga tcactatgta gcatgtgact ttccactgga
     1321 aagaaaagga gtagaataat gttagtatta aaaaatggaa cattaatgac catgtgcagt
     1381 aagacattta aaggtgacat tgctgttaaa gaaggtaaaa tagctgctgt tgattatgga
     1441 ttgcctacag aaaatgctca ggttcgtgat gtatcaggct gctatattat gcccggaata
     1501 attgatgcac atagtcatat cggtctacag gagagtggta cacgtgaaac ggatcataac
     1561 gagaaagcaa atccaatttc accgcagatg cgcgcaatag atgccattaa cccccaggat
     1621 atagcctttt cggaatcccg agaagtcgga ataactacct gcgcaaccgg gccgggcagc
     1681 attaatctga taggtggaac ctttgctgcg gtcaaaactt atggcaatac tgtagagaac
     1741 atgctactta agcatcccgt tgcaatgaaa gcagctttag gtgaaaaccc caagttccgc
     1801 tatacagaga tgggcatttc tccgaaatcc cgccttgctt ctgctgcaat tattcgtgct
     1861 gcgttagctg ctgccgtgga atatgacaga aaggcagaac tatatgatag cgattattta
     1921 caaatgccgg atagaaacct tggattggag gcactgctgc ctgtaattcg aggtgatttg
     1981 cctctcaaga ttcatgtaca ccgtgcggat gatattgcta cagctattcg tatagcagag
     2041 gagttcaata tacattttac tctggatcat tgttcagagg gttttatgat aacggatcgg
     2101 ctgctaagtg cattgagcaa gagatgtgac ggtattataa taggtccgtt aatgaattat
     2161 aaaagaaaat tggagtgcag taaaaagcag ggtgttaagc tcccaaaact tttgcatgac
     2221 gcaggtatag agtttgcaat ttgtacagat tttccggaaa tgatacctgt ttgtatgatg
     2281 cctcaaattg cactttcagt tacggaaggt ttgcctgaag atacagcact taaagcggtt
     2341 actattacgg cggcacgaat tttaggcata tctgaccgag ttggaagtct tgaagtagga
     2401 aaggatgctg atatagcagt tttttccggt cacccgctgg attatcgttc tgtatgtctg
     2461 atgacattga taaacggaaa aattgtatac gaaaggaaat gaaaaataat atgagtaaga
     2521 atgcaattaa atttacttac ttgtcacaag aggaacttat tgaagccggt gcatttaatt
     2581 taaaaatggc tattgaagca ttgcatcagg gactagtcaa gttcaaggat gaacaaattc
     2641 tttttcccga aaagattgta cagatattta acgaggaaac acaggaaagg ataaactgtc
     2701 ttcctgcaac gcttttagat gaaaaaatct gcggtgttaa atgggtatct tgcttccccc
     2761 ctaatcctat cagattcgga acgcaaaatc tgtctgcaat aatagttcta tcaaatatta
     2821 ttaatggtta tcctgtctgt gtcatggacg gcaccctatg ttcaaatatg cgcgttgcag
     2881 caatgggtgc aactgcagcg gataaacttg ccagggaaga tgcagaaagt attggattca
     2941 ttggtgccgg tgagcaggca aagatgcatc ttattggaat gaaagctgtg cgtccgggtt
     3001 taaaggtttg ccgggtcgct tcaaaatatg ccgaagaaga acatggtttc atttccggct
     3061 tgcaaaggat aattccggat atggaatttg ttgcttgtga tacggtactg gaaaaagcta
     3121 ttcgtgattc tgacatcatt gttactgcaa ccagtgcaca agcaccgctc cttaaagcgg
     3181 actggatcaa gaagggtgca ttttacagcc atgtaggagg ctgggaagat gagtacaaag
     3241 ttgtacaaat ggcagataag attgtatgtg acgattggaa tacggtaaag catagaactc
     3301 agacggtaag ccgatgctat aaggatggta ttattaatga ttcaaatatc tatgccaaca
     3361 taatagatat tatagatggt acaaagatgg gaagagaaaa tgatgatgaa tttatctatt
     3421 ttaatgccgt tggcttagct tatgcagata tttccatagc atattccatg tatagacatg
     3481 ctgtgaatca aggctttgga agggaacttg cccttcagga aacaatgatt ttcgataaga
     3541 acctggcagg gaaaatcgtt ttctgatcta tacctgtaaa ttgcatatat gaaaccgcag
     3601 cttgacttct aaagtgcact ttactataat aagtacgaaa cgaagaaagg caaggtatag
     3661 aaaacatgag gagattattc taatcaggag caactaaaca tttagtggat tcatccttat
     3721 ataagggttt gttctgctat tcattcagtt ccggttggga tacatgatcc tatgtttatt
     3781 atatataaca gtatgtttgc tgccatagac gtttattaac ccaggcatgc tttttattgc
     3841 tatgtatcct ctccggaaag caggagggga tttttttatt gtataagcaa gtgtaacgga
     3901 ttatacaata ggggatgcaa gggggagaat tttatgcttt tattagaagt caacaatata
     3961 aaaaaatatt acgccgacag gcttatacta gacataaaag attttaaggc atattacgga
     4021 gaaaaaatag gaattgtggg agcaaacggt gccggaaaga caacgcttct tgacattatt
     4081 gcagg
//
LOCUS       DAOSOW010000145         4082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_22025_length_4082_cov_4.394835, whole genome shotgun sequence.
ACCESSION   DAOSOW010000145 DAOSOW010000000
VERSION     DAOSOW010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4082
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22025_length_4082_cov_4.394835"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(811..2289)
                     /gene="spoIVA"
                     /locus_tag="PLD67_04750"
     CDS             complement(811..2289)
                     /gene="spoIVA"
                     /locus_tag="PLD67_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HOT21862.1"
                     /translation="MTNIDIYEDIAKRTKGEIYLGVVGPVRTGKSTFIRKFSENVMLP
                     NIEDEYTRERANDEMPQSGTGKTVMTTEPKFVPSDAVMVTFSNNVRAKLRLIDCVGYM
                     IPGAIGNMEGEVPRLVSTPWNDEPIPFAEAAEIGTKKVINDHSTIGVLVTTDGSITDI
                     NRDNYIMAEERVVRELKEINKPFVIILNTTHPKNENTVALSYQLKEKYDAPVLIVDCM
                     NLKTDDIENVLENVLYEFPIKEINVELPRWFDGLSYDYPIKRNLINILKDDFENYYKL
                     SDFKAEDIFSEENDYVEDYYVKDIELGTGKINLAVDLDNSYYYKIISDLCGEEITGEH
                     QILKLIGDLSKTRKSYSRVEAAINDAKANGYGYVTPTVSDMIIEPPEVFKEGGRYGIK
                     IKAQAPSLHIFSAGVNTEVSPVIGSKTQSEDLMCYLTEQMKENPQSIWQAEMFGKPLS
                     SLVEEQLDSKLTAMPDNARRKLRKTIEKIVNDGQGSIIFLIV"
     gene            complement(2467..3465)
                     /locus_tag="PLD67_04755"
     CDS             complement(2467..3465)
                     /locus_tag="PLD67_04755"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOT21863.1"
                     /translation="MNKKITVLGGGSWGTALSKLLSENGHNVTVWLRDEAQAKELTVK
                     RVNEKYLPKVKIPENIVFASDINKAVEEAEILLLVIPTQMVRGVLKQIKDEYKDGKII
                     INASKGIEKGTMKLVSTIVKEETKNTIFAVLSGPSHAEEVGLSLPTAITVACENKDVA
                     EEIQDIFMSEYFRVYTNEDVLGAELGGALKNIIALGAGISDGVGYGDNAKAALMNRGI
                     VEIARLGLAMGADLHTFYGLTGIGDLIVTCTSKHSRNWNAGYLIGQGLTKDEAIKKVG
                     MIVEGIPTTYAAYELSKKLMVEMPIVDAMYDVLENKADVKDTVNKLMLRDKKEERL"
     gene            complement(3475..4056)
                     /gene="plsY"
                     /locus_tag="PLD67_04760"
     CDS             complement(3475..4056)
                     /gene="plsY"
                     /locus_tag="PLD67_04760"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOT21864.1"
                     /translation="MKYIFIAIISYFIGNISFAYILGKILTNKDVREFGSGNAGTTNA
                     IRAFGKKVGAMVFSGDVLKGVVAVLFGRYFAGQTGAYIAGAFAIIGHNWPVLLNFKGG
                     KGVATTIGVMVFVNFYLTLICVIIGVIIIIFTRTVSLGSISGMAVAPLVAIILIRPLT
                     FDLLLFTIFVASMSIYRHKDNIKRLIKGNENKF"
BASE COUNT         1125 a          870 c          584 g         1503 t
ORIGIN      
        1 tgctcctttc gtcgcataaa ttgggaactc attgcggttg acctaccatc gatttcatat
       61 ttggggacat tcctcatgtc ctcaaagact tactacatag tagaggtgtg tccccatacc
      121 tatgtcgtcg aaaaaatcgg gttaggtcat actcaaaggt ttcttatgct gttttattgt
      181 ctatgttcga tttttttcgc tgccgttttt aagacagctt gaacagtcta tcatgccttt
      241 tggacaatgt caactgtcaa ttttttcttt ttttccttcc tgcttttttg gcaggaatgc
      301 tagattaaca cagtcttttt tctttgtcaa ccggcaattt ctgccagtct tctacgcctc
      361 tgccttttgt gacagctttg ttagtttacc ataactgcga attaaagtca ataatttttc
      421 actcattttt tctgtatttt tgacttgtct tctcagtttt ctttacaatc ttaatcaaaa
      481 aaactttcac aaaatctact gaaatttaaa tcataaatgc aatcggcttc ttggcaatca
      541 cttttttatt ttagctcact tatttacgct tgtcaatcag atttttatct atattttact
      601 gattgtcctt tatttccaat ccttaaatat taataatata ttatgcacgt ttattagatc
      661 gtttcaacat taatataata ttaatttata acacattttt attataataa aaaaatagat
      721 tttcatattt catttttcga aatagaaaat cttttatcga agaaattaag ttaggatata
      781 aaggacatat cgctgcttaa gtcaggcatc ttatactatg aggaatatta tgctcccttg
      841 accgtcgttt actatttttt caattgtctt tctaagcttc ctgcgtgcat tgtcgggcat
      901 agccgtaagc ttagaatcca gctgttcctc aacaagactt gataggggtt tgccaaacat
      961 ttcagcctgc catatacttt ggggattttc tttcatttgt tcggtaagat aacacattaa
     1021 atcctcgctt tgagttttgc ttcctatgac aggtgatact tccgtattga caccggcact
     1081 gaatatatgc aatgatggag cttgtgcttt aattttaatt ccgtacctgc cgccttcttt
     1141 gaaaacttcc gggggctcaa taatcatgtc gcttacagtc ggtgtcacat atccgtagcc
     1201 gttagcctta gcatcgttta tggcagcctc aacacggctg tagctcttcc ttgtttttga
     1261 caagtcacct ataagcttta atatttgatg ctcacctgta atttcttcac cgcaaaggtc
     1321 gctgattatt ttgtaataat aagaattatc caggtcaact gctagattga tttttcctgt
     1381 accaagttca atatctttta catagtaatc ttcaacataa tcattttctt ccgaaaaaat
     1441 atcctctgct ttaaagtcgc ttaatttgta ataattttcg aaatcatctt ttagtatgtt
     1501 aattaaattt ctttttattg gataatcata tgatagcccg tcaaaccatc ttggaagttc
     1561 aacattgatt tctttaatcg gaaattcata aagaacattt tctaacacat tttcaatatc
     1621 atcggttttt aaattcatac agtcgactat aagaaccgga gcatcgtatt tctcctttaa
     1681 ctgataagat aaagcaactg tattttcatt ttttggatga gtagtattta aaataataac
     1741 gaaaggctta ttaatttctt tcagttccct taccactctt tcttctgcca ttatgtaatt
     1801 atctctgttt atgtctgtga tgcttccgtc ggttgttact aataccccaa ttgttgaatg
     1861 gtcgttgatt accttttttg tacctatttc tgctgcttct gcaaagggta tgggttcatc
     1921 gttccaggga gtactaacaa gtcttggtac ttcaccttcc atatttccaa ttgcaccggg
     1981 aatcatatac cctacacagt caataagcct taattttgct cttacattgt tgctgaatgt
     2041 aaccattact gcatcactcg gtacaaattt tggctcagta gtcataacgg tcttgcctgt
     2101 accgctttga ggcatttcat catttgccct ttctcttgta tactcatcct caatattggg
     2161 cagcatcaca ttttccgaaa actttcttat aaatgttgac ttaccggttc ttaccggtcc
     2221 cacaaccccc aagtatattt cacccttggt tcttttagct atatcttcgt atatatctat
     2281 attagtgata ataatccctc cccttgcaaa tatgcactat tgtatacaat taatttatat
     2341 gccttgttca acttctttat tccaaaaaaa caaaaaaaga atatattcta atattctcta
     2401 tattcttctt ttaaattaca agagactcga ctgaagactt gtgacaatac ccccgtcccc
     2461 ttgtcactaa agtctttctt cttttttgtc tcttaacatt aatttattta ctgtatcttt
     2521 tacatcggcc ttgttttcca aaacatcata cattgcatcg actatcggca tttctaccat
     2581 tagtttctta gacaattcat atgcagcata tgtggtagga attccttcaa caatcattcc
     2641 tacctttttt atcgcttcat cctttgttaa gccttgaccg ataaggtagc ctgcattcca
     2701 gtttcttgaa tgcttgctgg tacatgtaac tatcaagtct ccaattccgg taagcccgta
     2761 aaatgtgtgc aaatccgctc ccatggcaag tccaagtctt gcaatttcaa caatacccct
     2821 gttcataagg gctgcttttg cattgtcccc gtagcctact ccgtcggata tacccgcacc
     2881 aagagctatt atatttttga gtgctcctcc taactctgca cctaatacat cttcatttgt
     2941 atatactctg aaatattcag acataaatat gtcttgaatc tcctcggcta catctttatt
     3001 ttcgcaggca acagttattg cagtaggcaa tgacaacccg acttcctctg catgggaagg
     3061 tcctgataaa accgcaaata ttgtattctt tgtttcttcc ttaacaattg ttgaaaccaa
     3121 tttcatggta cccttttcaa tacccttgga agcatttata attattttac cgtctttata
     3181 ctcatcttta atttgcttta atacacccct aaccatttgg gttggaataa ccaacaataa
     3241 aatctccgct tcctctacag ctttgtttat atcagaagca aatactatat tttcaggtat
     3301 tttaactttt ggcaagtatt tttcattaac cctttttacg gttaattctt tagcttgagc
     3361 ttcatctctc agccatacgg taacattatg cccgttttcc gaaagaagtt tggaaagtgc
     3421 ggttccccag cttcctccgc ctaggactgt tattttctta ttcatcctcg cctcctaaaa
     3481 tttgttttca tttcctttaa tcagtctttt tatattatcc ttgtgtctgt aaatggacat
     3541 acttgcaaca aatattgtaa ataataaaag atcaaaagta agcggacgaa tcaaaataat
     3601 tgccacgagt ggtgcaactg ccatgcctga aatagagccc aaggataccg ttcttgtaaa
     3661 aataataatt attacgccaa taataacaca aatcaaagtg agataaaaat ttacaaatac
     3721 cataacaccg atagttgtgg cgactccttt tcctcctttg aaattaagaa gaacaggcca
     3781 attgtgacct attattgcaa aagctcctgc aatataagct cctgtttgtc ccgcaaaata
     3841 tctgccaaac aagacagcaa cgacaccctt taatacgtct cctgaaaaaa ccatcgcgcc
     3901 tacttttttg ccgaatgccc ttattgcatt cgttgttccg gcattgccgc ttccaaattc
     3961 tctgacgtct ttattggtta atatttttcc taatatgtag gcaaatgata tattgcctat
     4021 aaaataagat attattgcga taaaaatata tttcataata cctccagcat aaccttatta
     4081 tc
//
LOCUS       DAOSOW010000146         4072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_22106_length_4072_cov_3.538462, whole genome shotgun sequence.
ACCESSION   DAOSOW010000146 DAOSOW010000000
VERSION     DAOSOW010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22106_length_4072_cov_3.538462"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..487)
                     /gene="nifU"
                     /locus_tag="PLD67_04765"
     CDS             complement(59..487)
                     /gene="nifU"
                     /locus_tag="PLD67_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HOT21865.1"
                     /translation="MYSEIVMDHFRNPRNVGHMEKPDGVGQVGNPKCGDIMKIYLKIK
                     DDIIEDVKFETFGCGSAIASSSIATELIKGKSIHEAVELTNKAVVEALGGLPPVKVHC
                     SVLAEQAVKSALYDYAKKANKVIKGLENYKPEDDEIHCQH"
     gene            complement(503..1690)
                     /gene="nifS"
                     /locus_tag="PLD67_04770"
     CDS             complement(503..1690)
                     /gene="nifS"
                     /locus_tag="PLD67_04770"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOT21866.1"
                     /translation="MSRKLYLDNAATTRVKKEVIDEMRQYFDILYGNPSSQLYELGRK
                     SKEAIEKARKTLADFLNAEEKEIYFTASGTESDNWALKGVAFANLNKGKNHIITTKIE
                     HHAILHTCEYLEKFGIETTYLDVDRYGLIDIEQLKESIRPETILISVMFANNEIGTIQ
                     PIEEIGKIAKDHGILFHVDAVQALGGIRIDVNKLHIDLMSMSAHKIGGPKGIGGMYIR
                     KGTRIDNFIHGGGQERGRRAGTEGVQNIVGFGKAVEIAARDFDKHVERLTVLRNRLID
                     GIKNNIPDVVLNGHPTQRLPNNVNFSYKFVEGESILLLLDMEGISASSGSACTSGSLD
                     PSHVLLATGLDHGTAHGSIRFTLSEELTEEDIDYTVETMKKIITRLRAMSPIKNDEDL
                     HKN"
     gene            complement(1668..2114)
                     /locus_tag="PLD67_04775"
     CDS             complement(1668..2114)
                     /locus_tag="PLD67_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOT21867.1"
                     /translation="MILSTKGRYGLKAAFELSKNYGLGPVSLKKISEKYGISENYLEQ
                     LFAKLKKSGYIDTVRGPQGGYLLAKKPEEITVGMILRTLEGEIKTSDCLGKEVCSRES
                     ICATRVIFEKIEKSINDVIDNITLADMYEEQKNILTEGRNEQETLS"
     gene            complement(2258..2518)
                     /locus_tag="PLD67_04780"
     CDS             complement(2258..2518)
                     /locus_tag="PLD67_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HOT21868.1"
                     /translation="MEVYIITFLHGNYAVAAFNRLQGFGVKNIHLIQTPFSIKNECDL
                     CIKTNDRKTLDIILNECRGRYPVSNVYTQINKNGSFVYKLLP"
     gene            complement(2598..3095)
                     /locus_tag="PLD67_04785"
     CDS             complement(2598..3095)
                     /locus_tag="PLD67_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21869.1"
                     /translation="MYKIFGKITDPNIENIISSKLNFDNLNVDQVYDIYVDFYNTDLE
                     EGMLNADISYEIKKEHYLFIKKIRALFENNQIRVNEFYLMGTISDLPENEINISIMKS
                     QGDKKKNVVWPCKEIFLYENQKNKLDALLISNQISEEDYESNLEFLKDELNIYENDEE
                     HKYIN"
     gene            complement(3107..>4072)
                     /locus_tag="PLD67_04790"
     CDS             complement(3107..>4072)
                     /locus_tag="PLD67_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOT21870.1"
                     /translation="FIDEIHRFNKAQQDALLPYVENGTIILIGATTENPYFEVNSALI
                     SRSLVFEFKRIDIEDIKNVVRKSLVDKRGYGNRKVEIDEDALAHIAEYANGDIRSALN
                     ALELGVETTEEIDGIIKINIDIASECIQRKKIDYDKDGDNHYDTISAFIKSMRGSDPD
                     AAVYWLAKMIKAGEDPKFIARRIIICASEDVGNADPRALEVAVNAFKAVEIVGFPEGR
                     IILAQAAIYIACSPKSNASIIAIDKAMNAVENESHEVPAHLKDKHYKGAEKLNRGIEY
                     KYPHDYDKFYVKQQYMPETINTKFYEPKESGYESRIKKFLEYLRQ"
BASE COUNT         1110 a          849 c          574 g         1539 t
ORIGIN      
        1 gctcaaatga taacaaaaag aaccgtcccc caaaagtcat tcactgtctt gtgggatctt
       61 agtgttgaca gtgtatctca tcatcctccg gtttataatt ttccaaaccc ttaattacct
      121 tgtttgcttt ttttgcataa tcataaaggg ctgattttac agcttgctct gccaataccg
      181 agcaatgcac cttaacaggc ggcaaccctc ctaaggcttc tacaacagct ttgtttgtca
      241 gttcaaccgc ttcatgaatg ctctttccct ttataagctc tgttgcaata cttgatgaag
      301 caatcgctga gccgcatccg aatgtttcaa attttacatc ctcaattata tcatctttaa
      361 tctttaaata tattttcatt atatccccgc atttggggtt acctacctgt ccaacaccgt
      421 ccggtttctc catatgacct acatttcttg gatttctgaa atggtccatt actatttctg
      481 aatacataat tccctccgta aattagtttt tatgcaaatc ttcatcgttt ttaatcggtg
      541 acattgctct taatctggta attatttttt tcattgtttc aacagtataa tctatatcct
      601 cttccgttaa ttcttcactt aaggtgaaac ggattgagcc gtgggctgtt ccgtggtcaa
      661 gccccgttgc caacaaaaca tgtgaagggt ctaatgaacc tgaagtgcag gcggaaccgc
      721 ttgaagctga tattccttcc atatccagaa gcaataatat ggattcgcct tccacaaatt
      781 tatatgagaa gtttacatta ttgggaagtc tctgtgtcgg atgcccgtta agcactacat
      841 cgggaatatt gtttttaatt ccgtcaataa gtctgtttct tagaactgtc agcctttcca
      901 catgcttatc aaaatccctc gcagcaattt cgacagcttt cccgaaacct acaatattct
      961 gtactccttc ggtaccggct cttctgcctc tctcttgacc gcctccgtga ataaagttat
     1021 caattcttgt tccttttctt atatacatac cgcctattcc cttaggtccg cctattttat
     1081 gtgcagacat tgacatcaaa tctatatgaa gcttattgac atctattctg atacctccca
     1141 aagcttgaac agcatctacg tgaaataata taccatggtc cttagcaatt tttcctattt
     1201 cttcgattgg ctgtatggta cctatttcat tatttgcaaa cattactgaa ataagaattg
     1261 tttcaggtct tattgattct ttcagctgtt ctatatcaat aagcccgtat ctgtcaacat
     1321 ccagataagt tgtttctatt ccgaattttt ctaagtattc acaagtatga agaattgcat
     1381 gatgctcaat ttttgttgtg attatatggt tctttccttt gttcagattt gcaaatgcaa
     1441 ctccctttaa agcccagtta tcagactccg taccgcttgc agtaaaatat atttcttttt
     1501 cttctgcatt caaaaagtct gccaaagttt ttcttgcctt ttcaatggct tcttttgatt
     1561 ttcttcctaa ctcatataat tgacttgatg gatttccgta caaaatatca aaatattgtc
     1621 tcatttcatc aattacttct tttttcaccc ttgttgttgc tgcattgtca agataaagtt
     1681 tcctgctcat ttcttccctc cgttaaaatg tttttttgct cttcgtacat atcagctaag
     1741 gttatattat caataacatc gttaatactc ttttctatct tctcaaatat aactcttgta
     1801 gcacaaattg attctctgga gcatacttct ttgcctaaac aatcagaagt ttttatttca
     1861 ccctcaagtg ttctgagtat cattccaaca gttatttcct caggtttttt tgctagaaga
     1921 tatcctcctt gaggtcctct gaccgtatct atgtaaccgc ttttttttaa ttttgcaaac
     1981 agctgctcta agtaattctc tgatatacca tacttttctg atattttctt tagagaaaca
     2041 ggtcctaatc cataattttt tgaaagttca aatgccgcct taagtccata tctgcccttt
     2101 gtggataaaa tcatttttac tctcctttca tggaatccct actgttttag tcgggtataa
     2161 ttcattatag aataccctag taattttgtc aacattaaaa aaaataaaaa taataaaaat
     2221 atcagcggat atttttatta ttttttgctt ataaatttta tggtaataat ttatatacaa
     2281 aggaaccatt tttgtttatt tgagtataaa cattgcttac gggatatcta cccctgcatt
     2341 catttaaaat aatatccaat gtttttctat cgtttgtctt tatgcataaa tcgcactcat
     2401 tctttatgct gaaaggagtt tgaatcaaat gtatattctt aactccaaat ccttgcagcc
     2461 tgttgaatgc ggcaacagca taattcccgt gcaaaaaggt aataatatag acttccatag
     2521 gatacctcct cgcctctatt ttattaatat tagcaaagaa ggttacaaaa aggggacatt
     2581 tctttgtccc ccttcctcta atttatatat ttatgttcct catcattttc atatatattt
     2641 agttcatctt tcaaaaattc taaatttgat tcatagtctt cttcagaaat ttgattgctg
     2701 ataagtaatg cgtccaactt gtttttctga ttttcatata aaaatatttc tttgcaaggc
     2761 cacactacat ttttcttttt atcaccctgt gatttcataa tcgaaatatt tatttcattt
     2821 tccggcaagt cggatatggt acccatcaaa taaaactcat ttactcgaat ttgattgttt
     2881 tcgaataaag ctctgatttt ttttataaac aaataatgct ccttcttaat ttcataagaa
     2941 atatcagcat ttagcatgcc ttcttccaaa tccgtgttgt aaaaatcaac atatatatcg
     3001 tatacttgat caacattcaa attgtcaaaa ttgagcttag agcttataat attttctata
     3061 ttcgggtcgg taatttttcc aaaaattttg tacatgctgc tcctccttac tgcctcaaat
     3121 attccaagaa tttcttaatt ctgctttcgt agcctgattc cttaggctca taaaatttag
     3181 tatttattgt ttcaggcata tattgttgtt ttacataaaa tttatcataa tcatggggat
     3241 atttatattc tattcccctg ttaagctttt cagcaccttt atagtgttta tcctttaaat
     3301 gagcaggtac ttcatgagat tcattttcaa ctgcattcat tgctttgtcg attgcaatta
     3361 ttgaggcatt gctctttgga ctacatgcaa tatatattgc ggcttgggca agaattatcc
     3421 gcccttcagg aaaacctact atttcaactg ccttaaatgc attcacggca acttccaaag
     3481 ctctggggtc tgcatttcca acgtcttccg aagcgcagat aattatcctt cttgcaatga
     3541 acttcgggtc ttctccggcc tttatcattt ttgcaagcca ataaaccgca gcatccgggt
     3601 cagagcccct catgctttta ataaatgcgg atattgtatc ataatggttg tctccatctt
     3661 tgtcgtaatc aattttcttt ctttgaatac attcagatgc aatatcaata tttattttaa
     3721 ttattccgtc aatttcttcg gttgtttcca caccaagctc taatgcgtta agagcgcttc
     3781 ttatgtctcc gtttgcgtat tctgcaatat gagctaaggc gtcttcatcg atttccactt
     3841 ttctgtttcc atatcctctt ttgtccacca atgattttct aaccacattc ttgatatcct
     3901 ctatgtcaat tcttttgaat tcaaatacca aagagcgtga aatcaaagcg gaatttactt
     3961 caaaatacgg attttcagtg gttgctccaa tcaatattat tgtaccattt tcaacgtagg
     4021 gaagcaaggc atcttgctga gctttattga aacgatgaat ttcatctatg aa
//
LOCUS       DAOSOW010000147         4065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_22154_length_4065_cov_3.402993, whole genome shotgun sequence.
ACCESSION   DAOSOW010000147 DAOSOW010000000
VERSION     DAOSOW010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22154_length_4065_cov_3.402993"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1547
                     /locus_tag="PLD67_04795"
     CDS             279..1547
                     /locus_tag="PLD67_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT21871.1"
                     /translation="MYIHVVQKGDVLWKIADRYNVSIQDIVDLNKLSNPNILLPGHAL
                     LIPTKNTYTVRRGDTIWKIAQKFGVNIQDLLAENRNINPDLIYPGNVLTIPQRPKPEI
                     DVNAFTYFLGSEAVPIVRGAGPYLTYLSPFAYLINEDGTLVNIADEPAIAEAYSQGVV
                     PMMAIVNFTVNVEGENLANIVLNNPDTIDTLQENIINIMREKGYQGLNIDFEYVLPED
                     REAYNSFLESTVNRLHNEGFFVSTSLAPKISGEQVGLLYEAHDYPAHGRLADFVVLMT
                     YEWGWRGGEPRAISPINEIRRVLDYAVTVIPRDKILMGFQIYARDWRIPFQEGDEAET
                     ISVQDAYNLAYEHMSEIHYDYVSQSPYFYYTDAEGNSHVVWFEDARSAQAKFDTVKQY
                     GLRGISYWGLGYEFPQNFPLLADNFQIKKL"
     gene            complement(1581..2906)
                     /locus_tag="PLD67_04800"
     CDS             complement(1581..2906)
                     /locus_tag="PLD67_04800"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOT21872.1"
                     /translation="MDFIIAVIIFIFSLIYNISKQYSLIIPLLIGMLAFSSVAFYRGF
                     KLRNIVVMLMKGMKKSLYILSIFALIGMITALWRAGGTIPFFVYYGIKIMNPDYFILF
                     AFLLTCFVAFALGTCIGTAGTVGVVLIILARSGGVNIYVAAGAILSGAFFGDRSSPLS
                     SSANLVAIITETDLYDNIKKMFITGALPFLATIVVYLLLSKNNPINVTNNELIEKFSV
                     NFNLHISTVLPAVIILLLAISKNDVRVAMLFSIITAVFICLYIQNLSIQEILKYMIFG
                     FVNTDPALSEIISGGGLISMLNVTFVVLIASSYSGIFEGTGMLKDIQGALIKMSNKIG
                     VYLTTVFTSLVTCACCCNQTLSVLLTHDLMNNIYKENNLTKDNLAIDIENTAILIAAI
                     FPWSVAVAVPLATMDVGAKAIFYAVYLFFVPLMAPLIPKSKVYNLGDGN"
     gene            3255..>4065
                     /locus_tag="PLD67_04805"
     CDS             3255..>4065
                     /locus_tag="PLD67_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002688524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT21873.1"
                     /translation="MDKDKLSRMHFSTRAVHAGYKPNEYGALATPIYQTSTFIFDSAE
                     QGGRRFALEEEGYIYTRLGNPTCSQVEDKLASLENAEAAVAMASGMGAITSVIWSIVS
                     AGDHIVAAKTLYGCTFAFMSHGLTKFGVEVDFVDTSDPENVRKAMKPNTKIVYLESPA
                     NPNMLLSDISVISKIAHETEGCMVVVDNTYCTPYIQRPIDLGADVVVHSTTKYLNGHG
                     DVIAGFAAGKKEFIDKVRLFGIKDMTGSVMSPFDAYLINRGMKTLEVRMEKH"
BASE COUNT         1409 a          660 c          794 g         1202 t
ORIGIN      
        1 aattaatttt gaaaaaatgg aatatagaac cgacattgct aaaatctaaa attatgaaca
       61 aaacatcgca aaaaacagat gacaataccc ccgtcccctt gtcagatttg ttgccggaga
      121 aattactctc cggcaacatt tatttttatc attataggtg gacaattgaa tatattataa
      181 catggagaga tatatatatg acaaggggac gggggtattg tcattaaaat tgcattacag
      241 tttcatgtta taataacaaa gaaataaagg gggaaactat gtatattcat gttgttcaaa
      301 agggagatgt attgtggaaa attgcagacc gttataatgt cagcatacaa gatattgtag
      361 atcttaacaa attgagtaat ccaaatattt tactgcccgg ccatgcactt cttataccaa
      421 caaaaaatac ctatacagtt agacgaggag atacgatttg gaaaatagcg caaaagtttg
      481 gggttaatat ccaagactta ctggcggaaa acagaaatat taatccggat ttgatatatc
      541 caggtaatgt acttacaata cctcaaaggc ctaagcctga aatagatgta aatgcattta
      601 catattttct tggaagcgaa gctgttccaa tagtaagagg agcaggaccc tatttaactt
      661 atttgagccc ttttgcatac ctgattaatg aagacggaac cttggtgaat attgccgatg
      721 agccggcaat agctgaagct tattcacaag gggtggtgcc catgatggca atagtaaatt
      781 ttacagtcaa tgtggaagga gaaaatcttg caaacatagt attgaataat cctgatacga
      841 ttgatacatt acaggaaaat attataaata ttatgagaga aaaaggatat cagggactaa
      901 acattgattt tgaatacgtt cttccggaag acagggaagc ctacaacagt tttttggaaa
      961 gcaccgttaa cagattgcat aatgaaggat tttttgtttc cacatcatta gctcctaaaa
     1021 taagcggaga gcaagtaggg cttttatatg aagcgcacga ctatccggct catggaagat
     1081 tggcagattt tgtcgttctc atgacctacg aatggggctg gaggggagga gaaccaagag
     1141 ccatttctcc gataaatgaa ataagaaggg tgttggatta cgcagtaact gtcattccga
     1201 gagataaaat attgatggga tttcaaatat atgcaagaga ctggagaatt ccttttcaag
     1261 aaggcgatga agctgaaaca atcagcgttc aggatgcata taatttagca tatgagcata
     1321 tgtctgaaat tcattatgat tatgtaagtc agtcaccgta tttttattat acggatgctg
     1381 aaggaaattc tcatgtagtt tggtttgaag atgcaagaag tgcacaggca aagtttgata
     1441 cagtgaaaca atacggttta agaggaataa gctactgggg gttaggatat gaattccctc
     1501 aaaacttccc cttgcttgcc gataatttcc aaatcaaaaa actatagggg caccggggac
     1561 gggataattt taattgtata tcaatttccg tccccaaggt tgtaaacctt ggatttgggt
     1621 atcaatggag ccattaaggg aacaaagaac agatatactg catagaatat tgctttagcg
     1681 ccaacgtcca tggttgcaag cggtacagct accgcaacgc tccacgggaa aatcgcagca
     1741 ataagaattg cggtattttc aatatcaata gctaaattat cttttgtaag gttattttct
     1801 ttataaatat tattcattaa gtcgtgggta agcaatacgg aaagtgtttg attacagcag
     1861 caagcacaag tgacgaggct tgtaaagact gttgttaagt aaacacctat tttattgctc
     1921 atctttatta aagctccctg aatatctttt agcatgccgg ttccttcaaa tatgccggaa
     1981 tatgatgatg ctatcaatac gacaaatgta acgttgagca ttgatattaa tccgcctccc
     2041 gaaataattt cggaaagcgc agggtcagtg ttgacaaaac caaaaatcat atattttaaa
     2101 atttcctgta tgctcaaatt ttgaatatac aggcatataa agacagcagt tattatacta
     2161 aaaagcattg ctacccgaac gtcatttttg gatattgcca acaagagaat tatgacggca
     2221 ggaagaactg ttgatatatg cagattgaaa ttaacggaaa atttctctat caattcatta
     2281 tttgtgacat ttatgggatt attttttgat aataacaaat agacaactat agttgcgaga
     2341 aatggcagag cgcctgtaat aaacatcttt tttatattat catacaaatc tgtttccgta
     2401 ataatcgcta ctaaatttgc actggaggat aagggcgagc ttctgtctcc aaaaaaagct
     2461 cccgataaaa tggcgcctgc tgcaacatag atgtttactc cgccgcttct ggccaatatt
     2521 atcaatacaa ctcccactgt tccggcagtt cctatgcatg taccaagagc aaatgcaacg
     2581 aaacatgtaa gtaaaaatgc aaataatata aaataatccg gattcataat ttttatgccg
     2641 taataaacaa aaaacggaat tgttccgccg gcacgccaca gagctgtaat cattcctatt
     2701 aaagcaaaaa tagacaatat atacagtgac tttttcatgc ccttcattaa cattacaaca
     2761 atgtttctaa gcttaaatcc cctgtaaaag gcaacggaag aaaaagcaag cataccaatc
     2821 agtaaaggaa taattaagga atattgtttc gaaatgttat atataagaga aaagataaaa
     2881 attatgactg ctattataaa gtccattaaa atctccatta aatatattta atataattat
     2941 ataatcattc cttaaaaaat caagcaaaat ccaattaggc aacattaaat tatatcctgc
     3001 agccccttat aataatttgc tgtccactta ttacctcttg gtgtttgatg aatttaagta
     3061 aaatacctta tgaaaccgct tttcctgggg caaaattttt ttatatgatg cactttgtta
     3121 tttaaatgag tggacggtac catttagatt aatggacaac tttttgataa atatggattg
     3181 atttatattt aaaagtagta tacaatttat atatagaaac gtttacttat cgtcattttt
     3241 tataaggagg aaatatggat aaggacaagt taagtagaat gcatttttct acaagggcgg
     3301 tacacgccgg gtacaaacca aacgagtatg gagctctagc aacaccaata tatcaaactt
     3361 ctacatttat atttgattcg gcagaacaag gcggaagaag atttgcattg gaagaagaag
     3421 gttatattta taccagactt ggaaatccaa catgctctca ggttgaagat aaattagctt
     3481 cacttgaaaa tgctgaagct gcagttgcaa tggcttcagg tatgggagcc attacatccg
     3541 ttatatggtc tatagtaagt gctggagacc atattgttgc ggctaagaca ttatatggat
     3601 gcacgtttgc tttcatgagc catgggttga caaaattcgg tgttgaagta gattttgttg
     3661 atacttcaga tcctgaaaat gttaggaagg ctatgaagcc taacactaaa attgtatact
     3721 tggaaagtcc ggcaaacccg aatatgcttt tgtccgatat ttcggtaatt tctaaaatcg
     3781 ctcacgagac agaaggctgt atggttgtag tggataatac ttattgcaca ccttatatac
     3841 aaagaccaat tgatcttggt gcagatgtgg ttgttcattc tacaacaaag tatttaaatg
     3901 gccacggtga tgttatcgcc ggttttgcag ccggtaaaaa agaatttatt gacaaggtaa
     3961 gactatttgg aataaaagat atgacaggct cggttatgag tccttttgat gcatacttga
     4021 ttaacagggg aatgaaaaca ttagaagtca gaatggaaaa acact
//
LOCUS       DAOSOW010000148         4020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_22516_length_4020_cov_3.654224, whole genome shotgun sequence.
ACCESSION   DAOSOW010000148 DAOSOW010000000
VERSION     DAOSOW010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22516_length_4020_cov_3.654224"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="PLD67_04810"
     CDS             complement(<1..654)
                     /locus_tag="PLD67_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003503258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HOT21874.1"
                     /translation="MSTSKKTVWENYRFSVILIISIAIGSFIGLVFGDRAVILKPFGD
                     IFLNLMFTAVTPLVFVTIASAVGSMVNMRRLGRILGNMLLVFVITGLFAAVLIIFVVS
                     VYPPAEGVNIQIEAAGELEKLTFADQAVSAITVNDFSKILSRSNMLPLIVFSILFGYC
                     VSAVGGEDNIVARALDALSKVMMKFISVIMLYAPIGLGAYFASLIGEFGPQLLGAYAR
                     "
     gene            complement(676..1512)
                     /gene="dapF"
                     /locus_tag="PLD67_04815"
     CDS             complement(676..1512)
                     /gene="dapF"
                     /locus_tag="PLD67_04815"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOT21875.1"
                     /translation="MEFCKMQGAGNDFIIINNMKLNIPVDKLPAIAKKLCQRKVSIGA
                     DGLMAVDIPDGDADFKMLFFNRDGSIGEMCGNGARCISRYAYINNIAGKKMIFETGAG
                     IVSAEVLEGRLVKVMLNNPEVINLYNDIEIDGIKYELSYIELGNPGLPHAVVKYNLKQ
                     ADENKLREEGIKIRHYEGFPKGTNVNFFEVLDENTALVKTYERGVEDFTLACGTGSAS
                     TALALILKGYLKNNQAKMINDGGELIIEVEKTDNKIEKLFLIGDTNIVAIGKIMDEDL
                     II"
     gene            complement(1532..2830)
                     /gene="lysA"
                     /locus_tag="PLD67_04820"
     CDS             complement(1532..2830)
                     /gene="lysA"
                     /locus_tag="PLD67_04820"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOT21876.1"
                     /translation="MVREIKGNTLYFDGCNTVELANKYGTPLYVMSETAMVDKCKEIK
                     DTFLNKYERTRAAYASKAFLTLAMCKIIEREGLCLDVVSGGELYTAIKADFPAERIEF
                     NGNNKSIEELELAIDYNIGRIIVDGFDELGIIEDICKKKGKKTNILYRITPGVKSDSH
                     DYIVTGKKDSKFGFPLDDDVIFPAIEKAINSPYINFLGFHFHVGSQLHNNESHLKALK
                     ISLKLIKDTIDKYNYVTQELNVGGGFGIRYTDADDKKPYAYFLDPMMAEIEEFSKRMD
                     IKRPEIVIEPGRSIVGEAGITLYTIGTIKDIEGIRKYVSVDGGMTDNIRPALYQAKYE
                     CVIANKAAQPSTDLVTISGKCCESGDILIRDAYIPLAERGDILAIFSTGAYGYAMASN
                     YNKNPFPATVLVKEGKSEVIVKRQTYEHMIENEVIPKSLV"
     gene            complement(3026..3403)
                     /locus_tag="PLD67_04825"
     CDS             complement(3026..3403)
                     /locus_tag="PLD67_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HOT21877.1"
                     /translation="MASIEEVQIILDEIAEELPPEIYKDLNGGIILLPQVKYHKKSVD
                     NDLYVMGEYRNEMISGRYIIVYYGSVERLYGNLTRNRLKNKLRSVVKHEFLHHLESLA
                     GEKHLEIEDERKLSEYLKKKDSF"
     gene            complement(3708..>4020)
                     /locus_tag="PLD67_04830"
     CDS             complement(3708..>4020)
                     /locus_tag="PLD67_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21878.1"
                     /translation="YFDGSWTTFSKNSLDCRDYNFIVRNGNLAKMQILNERCEYFLKG
                     SEIVTIFSMDDFVIYLEDFDEKIDISAFSLLILEIEDEEKISVISADSPLILTEFFIT
                     K"
BASE COUNT         1225 a          828 c          533 g         1434 t
ORIGIN      
        1 tcttgcatat gctccaagaa gctgcggtcc gaattctcct atcaaactcg caaagtacgc
       61 tccaagtcct atcggtgcgt aaagcataat tacacttata aatttcatca taactttaga
      121 taatgcatct aaagcccttg ctacaatatt gtcttctcct cctactgcac ttacacaata
      181 gccgaaaaga atagaaaata caattaaggg cagcatattg gaacgtgata aaatcttgct
      241 gaagtcattc actgtaattg cagaaacggc ttggtcagca aacgttaatt tttctaattc
      301 tccggcggct tctatctgta tgtttactcc ttctgcagga ggatatacac taacaacaaa
      361 gataattaaa acagctgcaa ataaaccggt tattacaaat actaaaagca tgtttcccaa
      421 tatcctgcca agacgcctca tattgaccat actgcctaca gcacttgcaa ttgtaacaaa
      481 tactaatgga gtaactgctg taaacatgag attcaaaaat atatctccaa agggtttcaa
      541 tattacggct ctatcaccaa atacaagacc gataaaacta ccgatagcta ttgaaataat
      601 caatattacg gaaaacctgt agttttccca tactgttttt ttagatgtac tcatattatt
      661 ctcctttaac aatttttaaa taattaaatc ttcatccata atttttccga ttgctactat
      721 gttagtatca cctataagaa acagtttttc tattttattg tctgtttttt caacttctat
      781 aatcagttcc ccaccatcat ttatcatttt agcttgatta tttttcaaat agccttttag
      841 tattaaggca agggctgttg acgcagagcc ggtgccgcaa gctaatgtaa agtcttctac
      901 ccccctttca taggttttta ccaatgccgt attttcatcc agcacctcaa aaaagttaac
      961 atttgtaccc tttggaaagc cttcgtaatg ccttattttg attccttctt cacgtaattt
     1021 gttttcatcc gcctgcttaa ggttatactt aactactgca tgaggaagcc cgggattgcc
     1081 taattctata tatgataatt catattttat tccgtctatt tcaatatcat tatatagatt
     1141 aattacttcc ggattattta gcataacttt taccaaacgc ccttctaaaa cttcagcgct
     1201 gacaattcct gctcctgttt caaaaatcat tttttttcct gctatattat ttatatatgc
     1261 atagcgagaa atacatcttg ctccattgcc gcacatttct cctatgcttc cgtctcggtt
     1321 aaaaaaaagc atcttaaaat ccgcatctcc atcagggata tcaaccgcca tcaacccgtc
     1381 tgctccgatt gatacttttc tttggcatag tttttttgca atggcaggta atttatctac
     1441 aggaatattc aacttcatat tattaataat tataaaatca ttccctgctc cctgcatttt
     1501 gcaaaattcc ataagcgctc acttccttat tttatactag cgatttcggt ataacttcgt
     1561 tttcaatcat atgttcatat gtctgccttt ttactattac ttccgatttt ccttccttga
     1621 ccaggactgt tgcaggaaaa gggtttttat tgtagttgct tgccatagca taaccgtatg
     1681 ctcctgttga aaatatggca agtatatctc ctctttcagc taatggtata tatgcatccc
     1741 ttattaatat atcccccgat tcgcagcatt ttccgcttat tgttactaaa tcggtgcttg
     1801 gctgggctgc tttgttagca ataacacatt catactttgc ctggtataat gcgggtctta
     1861 tattgtcggt cattccgccg tcaacggaca catattttct tataccttca atatccttta
     1921 tagtgccgat tgtataaagt gttattccgg cctctcctac aatacttctg cccggctcga
     1981 taacaatttc aggtcttttg atatccattc tctttgaaaa ctcttcaatt tccgccatca
     2041 tggggtctaa aaaatatgca tagggcttct tatcatcagc atctgtatac cttattccaa
     2101 atcctccgcc gacatttaat tcttgtgtta cataattata tttatcaatt gtgtctttta
     2161 ttaattttaa tgaaattttt aatgctttta aatgtgattc attgttatga agctgtgagc
     2221 ctacgtgaaa atgaaaaccc agaaaattta tgtagggtga attaattgct ttttcaattg
     2281 caggaaatat aacatcatca tccaacggaa atccaaactt ggaatccttt ttccctgtaa
     2341 ctatataatc atgagaatca cttttaacac ccggagttat tctgtaaaga atatttgtct
     2401 ttttcccttt tttcttacaa atatcttcaa taattcccaa ttcatcaaag ccgtccacaa
     2461 ttattcttcc aatattgtaa tctattgcaa gttctaattc ttcaattgat ttgttattgc
     2521 cgttaaattc aattctttct gcaggaaaat cggcctttat tgctgtgtac agttctcctc
     2581 ccgatactac atccaaacaa aggccttctc tttcaattat cttgcacata gcaagagtta
     2641 aaaatgcttt tgaagcatat gcagcccttg ttctttcata cttgtttaaa aatgtatctt
     2701 ttatttcttt gcacttatcg accattgccg tttcactcat cacatacagc ggcgtcccat
     2761 acttgtttgc caattcaacc gtattgcatc cgtcaaaata caatgtatta cctttaattt
     2821 ctcgcaccat attatctcct tataaatttt gtgttagtat taaaaattat aacgctttat
     2881 ataataatta tgcaatatta taagcagcct ttacctttaa atcaagataa attattgaaa
     2941 taaaaaaaac ggctctataa atcaaaaata aaaaccgtat ttctatgttt acaatagtat
     3001 taatgaagga attcaattca tataattaaa aagaatcctt ctttttcaga tactctgata
     3061 atttacgttc atcttctatt tctaaatgct tttctccggc caatgattct agatgatgca
     3121 aaaattcatg tttgacaact gacctgagtt tatttttaag cctgtttctt gtaagattgc
     3181 cataaagcct ttcgacagag ccatagtata caattatata ccttcctgaa atcatttcgt
     3241 tgcggtattc acccatcaca taaagatcat tatcaacgct ttttttatga tattttactt
     3301 ggggcagcaa aataatacct ccgttaagat ctttgtaaat ttcaggaggt aactcctctg
     3361 ctatttcatc caatataatt tgtacttctt ctattgacgc catatgccat aataatcctt
     3421 tatattaagt attacctata ttattcgcct ataattttaa tcaccaaggg gtatgcctat
     3481 atactcctaa tatttataat aacaccagta agcagctttg tcaaaacatg taaattctta
     3541 tggtgactaa actttttggc ggtaccggag caatactttg cacagttgac aggctatcag
     3601 catttgacag tcaaaatctt attataccta tatgggcaat ttagtaaaga aaacaaaaaa
     3661 taagaaagga aaaagttgta ggctgcaaaa cctacaactt ttaacaatca cttagtgata
     3721 aaaaactcag ttagtataag tggtgaatca gcagatatta cactaatctt ttcttcatct
     3781 tctatttcta aaatcagtaa tgaaaacgca ctgatatcta tcttctcatc gaaatcttct
     3841 aaatatataa caaaatcatc catagagaat atagttacta tctccgatcc cttaagaaaa
     3901 tactcacatc tttcatttag aatttgcatc tttgcaagat tcccatttct tacgatgaag
     3961 ttataatctc tacagtcaag actgtttttg gaaaatgtgg tccagcttcc atcaaaatat
//
LOCUS       DAOSOW010000149         4019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_22525_length_4019_cov_3.530020, whole genome shotgun sequence.
ACCESSION   DAOSOW010000149 DAOSOW010000000
VERSION     DAOSOW010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22525_length_4019_cov_3.530020"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1526)
                     /locus_tag="PLD67_04835"
     CDS             complement(<1..1526)
                     /locus_tag="PLD67_04835"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOT21879.1"
                     /translation="MSDKFLSDIEIAQNAEIEVINKIADKIGIPDKYVENYGKYKAKI
                     DYRYFQDELKNNEDAKLILVTAISPTPAGEGKTTTTIGLGDSLSKLGKKTIIALREPS
                     LGPVFGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNQLD
                     IDIRRIVWRRAVDMNDRQLRNIVDGLGGKADGYPREDGFDITVASEVMAVFCLSNDII
                     ELKENLSKIIVAYNRAGEPVTAGQLKAHGAMAALLKDALKPNLVQTLEGTPAFIHGGP
                     FANIAHGCNSVIATKMASKIADYAVTEAGFGADLGAEKFIDIKCRKTGLKPSAVVIVA
                     SVRALKHHGGVAKADLGKENLEALDKGIPNLLKHVENITQVFKIPAVVAINKFPTDTE
                     AELNLIKEKCMELGVNVRLSEVWGKGSEGGIELAKEVVRLTEQESSLEFAYDLDIPIK
                     EKIRTIATKLYGADDVEFSSKAAKEIANFEKLGFGNLPICMAKNQYSLTDDSKIFGRH
                     T"
     regulatory      1654..1747
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            1813..2556
                     /locus_tag="PLD67_04840"
     CDS             1813..2556
                     /locus_tag="PLD67_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HOT21880.1"
                     /translation="MSSNTEPQKFTSLTVVRAGLFISMSLVLKVMFEVYIPLAGIPAL
                     RINFANIPLMLSGIILGPFTGFLTGAAADIINFIAKPGGPFFPGFTLASGLSGFIPGI
                     IFKYFKRDINYNLVNTTFISLLSAGFILVFMQNGLLTFDNGILYDGKPLNPLYAAGFF
                     VLVAVYLIIPIKLSSATKDLSMNKIVFAISITQLITSIILNTYFLYILYGIGIMITLP
                     ARILTNFFMIPLYSIIIGAILNAVKGRIK"
     gene            complement(2656..3570)
                     /locus_tag="PLD67_04845"
     CDS             complement(2656..3570)
                     /locus_tag="PLD67_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21881.1"
                     /translation="MRKFILFITVFTLMLTIGCTQIPKSEETGLGPEGYEGIASTEEI
                     KKENSSLKEELEKIKTEFEVLEENYLRLVKNNESIISKLEETESKLEIISSEDIPEFK
                     SEETDKDSIVSYLNDTSKLIDDSIKGIEIISSGESVVFRTLGYGEEYSQIFIWDEGEN
                     EPVLIDGAVIEKGGSYEWLGKYILIKNKDKNKVLDIENKKITGSFDEPQKLQLLEGTS
                     AILLKDKDNNFVLYDFINDNSKQINLDNNKYTDFDLRNNIIVFSGAYVENDVEYEIRA
                     SIALDKLIEAFDTEGSEEAESEEIEDSV"
     gene            complement(3623..>4019)
                     /locus_tag="PLD67_04850"
     CDS             complement(3623..>4019)
                     /locus_tag="PLD67_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21882.1"
                     /translation="DGEIKQYSVSSNVKITIGAKNVSIYDLRLGYNVYINTSGDEIVA
                     MEVSEMETAKSFTGKIIYINEDDGLIMMQSVTSTGKELIYLSVTNSTIIYDTSGKTRY
                     FKDLEEGESIVSIAVQQGGEYIATSIMIQ"
BASE COUNT         1129 a          819 c          592 g         1479 t
ORIGIN      
        1 gtatgtctgc cgaatatctt tgaatcatca gtaagagaat attggttttt agccatgcat
       61 attggaagat ttccaaaccc gagtttttca aaattagcaa tttcctttgc tgcttttgat
      121 gaaaattcca catcatctgc accataaagt tttgttgcaa ttgttcttat tttttccttg
      181 attggaatat ctaagtcata ggcaaattcc aatgaacttt cctgctctgt tagtctaact
      241 acctcttttg caagctcaat tccgccttcg cttcctttac cccatacttc tgacagcctt
      301 acatttacac ccagctccat gcatttttct tttatcaagt ttaattctgc ctcagtatct
      361 gtaggaaact tatttattgc aacaacagca ggtattttaa acacctgagt aatattctca
      421 acatgcttca ataaattggg aattccctta tccaatgctt ccaagttctc ttttcccaaa
      481 tctgcttttg ctacccctcc atggtgtttc aaagctctaa cagatgcaac tataactact
      541 gcacttggtt ttaaaccagt ctttctgcat tttatatcta taaatttttc tgctcccaag
      601 tctgctccga aacctgcctc tgttacagca tagtctgcaa tttttgatgc catttttgta
      661 gcaattactg aattgcagcc gtgagctatg ttagcaaaag gacctccatg aataaatgca
      721 ggcgttcctt cgagtgtttg aaccaaatta ggctttaatg catccttcaa aagtgcagcc
      781 atggctccat gtgcttttaa ttgacctgcc gttacaggct ctcctgctct gttatatgca
      841 actattattt tagataaatt ttctttaagt tcaattatat cattagaaag gcaaaatacc
      901 gccattactt cagatgcaac cgtaatatca aatccgtctt cccttgggta accatccgcc
      961 ttgccgccta aaccgtcaac tatattcctt aactgtctgt cattcatatc aacggctctt
     1021 ctccatacaa ttcttctgat atcaatgtcc aattggttcc cttggtgaat atggttatca
     1081 agcattgctg caagaagatt gttggcagcc ccgatagcat gaaaatctcc ggtaaaatga
     1141 aggtttatat cttccatggg aacaacctga gcatatcctc ctcctgctgc tcctcctttt
     1201 actccaaaaa ccggtcccag ggagggctca cgaagggcaa ttattgtttt ctttccaagc
     1261 ttagatagtg aatctccaag tccaatggta gtagtagtct tcccttcccc ggcaggagtg
     1321 gggcttattg cggttaccaa aataagctta gcatcttcat tgttttttaa ttcatcctga
     1381 aaatatctgt aatcaatttt tgctttgtac tttccgtaat tttcaacata tttatccgga
     1441 atacctattt tatccgctat tttatttatt acttctattt ctgcattttg tgcaatttct
     1501 atatcgctta aaaatttatc tgacataaag cctcctcatt tgtgcgtttt tattattata
     1561 gcataaaaaa gtaaagttac aacaaaaaag ctatccttac aataaaaata acagtataca
     1621 tatttattat tatatgttat tatgtataaa aaattcagag tagataagat gcgttaagtg
     1681 ttgagggatg ggatgtcgcc ctcaaacgaa agacttatgt ctgcggtttc ttatcgcttc
     1741 cgctgtatta gagaaacata tgttgctcct atattgtgcg gaggtattgc tttgctcaat
     1801 aaggaggaaa atatgagtag taatactgag ccacaaaaat tcacatcatt aactgttgta
     1861 agagcagggc tttttatatc aatgagcctt gtgttaaaag taatgtttga agtatatatc
     1921 cctttggcag gaataccggc acttaggata aacttcgcta acattccgtt gatgctttca
     1981 ggcataatat taggaccgtt tacgggattt ttaaccggag ccgctgcaga tataatcaac
     2041 tttatcgcaa agcccggagg gccctttttc cccgggttta ccttagccag cggattaagc
     2101 ggtttcattc ccggaattat ttttaaatat tttaaaaggg atataaatta taatttagtc
     2161 aatacaactt ttatttcttt attgtctgcc ggttttattt tggtttttat gcaaaatgga
     2221 ttattgacat ttgacaatgg tatactttat gacgggaagc ctttaaatcc tttatatgca
     2281 gcagggttct ttgtgcttgt agctgtatat cttataattc caattaagtt gtcatctgca
     2341 acaaaagacc tgagcatgaa taaaatagtt tttgcaatca gtataactca gctgattaca
     2401 tcaataattt tgaacactta ctttttatat attttatatg gcataggtat aatgattacc
     2461 ctgccggcga gaatattgac aaacttcttt atgatacctc tttattcaat aatcatcggt
     2521 gctatattaa atgctgtaaa gggaaggatt aaataaaaat ctttcacagg ttattcatag
     2581 caatgacgaa agcttattat ttgaaaaatt aaagggcagg taataaatct gcccttatct
     2641 ttgtacctta atacattaaa cactatcttc tatttcttct gattctgctt cttcgcttcc
     2701 ttccgtatca aatgcttcaa ttaatttatc caaagcaatg gatgccctta tttcatattc
     2761 cacatcattt tcaacataag cacctgaaaa tacgattata ttgtttcgta aatcaaaatc
     2821 agtatattta ttattgtcta aattaatttg tttactgttg tcatttataa aatcatacag
     2881 aacaaagtta ttatccttgt ctttaagcaa aattgcagaa gttccctcca ataattgcaa
     2941 tttttgcggc tcatcgaagg agcctgttat tttcttgttt tcaatatcta ataccttgtt
     3001 cttatcctta ttcttaatta aaatatactt gccgagccat tcataggagc ctcccttttc
     3061 aattactgct ccgtcaatca acaccggctc attttcccct tcatcccaaa tgaatatttg
     3121 actgtactct tctccgtatc ccaaggttct aaatactaca ctttcaccgg aacttattat
     3181 ttcaattcct tttatactgt catcaattaa ttttgaggtg tcattcaagt atgaaacaat
     3241 gctgtccttg tctgtttctt ctgatttaaa ttcaggaata tcctcactgc tgattatttc
     3301 taattttgat tctgtttctt ccaacttaga tataatactt tcattgtttt tgactagtct
     3361 taaataattc tcttctaata cttcaaattc tgttttaatt ttttccaatt cttcttttag
     3421 gctgctattt tcttttttaa tttcttcagt agaggcaata ccttcatatc cttcgggccc
     3481 taaacctgtt tcttcagatt tgggtatctg agtacagcct attgtaagca ttagggtaaa
     3541 aactgttata aacaatataa attttctcat actctcacat cctttcgcat ccctatagag
     3601 aaaaaagcat ctttcgatgc ttttactgaa tcattatact tgttgctatg tattctccgc
     3661 cctgttgaac agctatactt acaatacttt ctccttcttc caaatctttg aaatacctgg
     3721 ttttacctga agtatcatat attatcgtgc tgttggtaac tgacaggtaa atgagttctt
     3781 tccctgttga cgtgacactt tgcatcatta ttaatccatc atcttcatta atatatataa
     3841 tcttaccagt gaagctcttg gcagtttcca tttctgaaac ttccatcgca acaatttcat
     3901 ctcccgaggt gtttatataa acattatatc caaggcgcaa atcgtaaatc gatacatttt
     3961 ttgctccgat tgtaatctta acattgctgc tcacggagta ctgcttaatt tctccatct
//
LOCUS       DAOSOW010000150         4001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_22657_length_4001_cov_3.291181, whole genome shotgun sequence.
ACCESSION   DAOSOW010000150 DAOSOW010000000
VERSION     DAOSOW010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22657_length_4001_cov_3.291181"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..561)
                     /locus_tag="PLD67_04855"
     CDS             complement(115..561)
                     /locus_tag="PLD67_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOT21883.1"
                     /translation="MLFSETMTMGERFVNGLAITILGMSVVFVVLVIIAYSLDLLKVL
                     FGEKDKKNSADNKVDSKKVLIKAEESINTTPNVSESEIDPNLLAVLTAAIAVQNTDEL
                     IAVLTAAILAQSGSTEEMIIKSIMPVKQKRSIWASAGRQEQMLKTI"
     gene            complement(595..2139)
                     /locus_tag="PLD67_04860"
     CDS             complement(595..2139)
                     /locus_tag="PLD67_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOT21884.1"
                     /translation="MSNKTEEVYSRKSKLKQGGGPKRIEKQHAQGKLTARERLNLLFD
                     EGSFREIGLFVKHRCTDFGMEEVETPGEGVVTGYGTVDGRLVYAYAQDFTVLGGSLGA
                     MHAKKITTVMDLALKMGAPIIGMNDSGGARIQEGVDALSGYGSIFYRNTISSGVIPQI
                     SAIMGPCAGGAVYSPAITDFTFMVDKTSQMFITGPQVIKAVTGEEISPEALGGAMAHN
                     AISGVAHFVSANDEACIQEIKRLLSFLPSNNLETSPLVETNDDLNRVEEKLNDIVPEN
                     PNKAYDVKEIIQTIADNNDFLETQPYYAKNIVTGFIRINGQTIGVIANQPRHLAGCLD
                     IDASDKSSRFIRTCDCFNIPLLNLVDVPGFLPGSAQEHGGIIRHGAKMLFAYSEATVP
                     KVTLILRKAYGGAYLGMCSKELGADVVLAWPSAEIAVMGPDGAANIIFKREIENADNP
                     EEERKAKIEEYKEKFSNPYVAAARGFVDDVIEPSITRPILASAFEMLSSKRETRPAKK
                     HGNLPV"
     gene            complement(2359..2760)
                     /gene="mce"
                     /locus_tag="PLD67_04865"
     CDS             complement(2359..2760)
                     /gene="mce"
                     /locus_tag="PLD67_04865"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOT21885.1"
                     /translation="MVKKVDHIGIAVKNLDEALNFYENILGLKSAGTETVEEQKVRVA
                     FLPLGDTEVELLESTEEDGPIARYIAKNGEGIQHIAYRVDDIEKAIAEMKENGIRMID
                     EKPRYGAGGAKIAFCHPKSTFGVLIELCQRD"
     gene            complement(2775..3722)
                     /gene="meaB"
                     /locus_tag="PLD67_04870"
     CDS             complement(2775..3722)
                     /gene="meaB"
                     /locus_tag="PLD67_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOT21886.1"
                     /translation="MEYKQLVEKMLIGDKRACAKLITAVENEPEKAEKIIREIHQYTG
                     NAHIIGITGPPGAGKSTLTDKIVKALVSEGNKVGVIAIDPTSPYSGGSILGDRIRMQD
                     LALNPDVFIRSMGTRGYLGGLSKYTKNALKVLDAAGYNYIIIETVGVGQSEVDIVKIA
                     DTIVMVMVPGLGDDIQSIKAGIMEIGDIFAINKSDLFGADRTAVEVNMMLDLTMDMDR
                     RPPVVKVVAVNNEGIDDLLNEIKEHIKYLKASGKFEERRKNNLKLEVVELIEEELRKI
                     VISQTDSENRLDSEVIEILNKNKNVYEVRDEFLKVLNDI"
     gene            complement(3798..>4001)
                     /locus_tag="PLD67_04875"
     CDS             complement(3798..>4001)
                     /locus_tag="PLD67_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOT21887.1"
                     /translation="SGAHNHLFPRVAELLKEQGVDDVLIIGGGVIPDDDIQGLKEAGI
                     KEIFTPGTETSKMIEYIKNNVQR"
BASE COUNT          959 a          920 c          601 g         1521 t
ORIGIN      
        1 gctgaattat ttgttgtatt actggttgat ttcctcttac ttcttccgcc attacttcgt
       61 atgttacgcc attaactgtt attaaatatt ttttcatgat ttaatctcct tttgctatat
      121 tgtttttaac atttgttctt gtcttcctgc cgaagcccat atacttctct tttgcttaac
      181 aggcattatt gatttaataa tcatttcttc tgtgcttccg ctttgagcta atattgctgc
      241 tgtcagcact gctattaatt catctgtatt ctgaactgca attgctgctg tcaacactgc
      301 cagtaaattt ggatctattt ccgattctga tacgtttgga gttgtattaa tactttcttc
      361 agcctttatt agcacttttt tactatcaac tttattatca gctgaatttt ttttgtcttt
      421 ttctccaaac aaaactttta acaaatcaag ggaataggct ataataacca gtactacaaa
      481 cactactgac attcccagta ttgttattgc caagccattt acaaatctct cccccattgt
      541 cattgtttcg ctgaatagca tttaatcacc tcttttgttt ttacttgtat agagttatac
      601 aggaaggttt ccatgttttt tggccggtct tgtttccctc ttgcttgata gcatttcaaa
      661 tgcacttgca agtatgggtc tggttatcga cggctcaatt acatcatcta caaatcctct
      721 tgcagcagct acatatgggt ttgagaattt ttctttatat tcttcaattt ttgcttttct
      781 ttcttcttcc gggttatcag cgttttcgat ttctcgttta aatattatat tcgctgctcc
      841 gtcaggtccc attacagcaa tttctgctga cggccatgcc aatacaacat ctgcccctaa
      901 ttcttttgaa cacattccta agtatgcacc gccatatgct tttcttaata ttagtgtaac
      961 ctttggaact gtagcttcgg aatatgcaaa gagcattttt gctccatgtc ttataattcc
     1021 tccgtgttct tgtgcagaac ccggtaaaaa tccaggcacg tcaaccaaat ttaacaacgg
     1081 aatattgaag caatcgcatg ttctgataaa tctgctcgat ttgtccgatg catctatatc
     1141 aaggcatcct gctaaatgtc tgggctggtt tgctataact cctattgtct gtccgtttat
     1201 tcttatgaat ccagttacaa tattcttagc atagtatggc tgtgtttcca agaaatcgtt
     1261 attgtctgct atagtttgta ttatttcttt tacgtcgtat gccttgtttg gattttcagg
     1321 aactatgtca tttaattttt cttctactct gtttaaatcg tcattcgttt ccaccagcgg
     1381 tgatgtttcc aaattgttag acggcaaaaa gcttaatagt ctttttattt cctgtatgca
     1441 agcttcatcg tttgcactta caaaatgagc tactccgctt attgcattgt gtgccattgc
     1501 tccgcctaat gcttccgggc tgatttcttc gccggttaca gccttaatta cctgcggtcc
     1561 cgtaataaac atctggctcg ttttatctac cataaaggtg aagtctgtta ttgcaggtga
     1621 ataaacggct cctccggcgc atggacccat tattgccgat atttgcggaa ttactcctga
     1681 tgaaattgta tttctgtaaa atatgcttcc gtatccgctc aaggcatcca cgccttcttg
     1741 tattcttgct ccgcctgaat cattcatacc gattatgggt gctcccattt tcaaagctaa
     1801 atccataact gtagtaattt tcttcgcatg catggcacca agggatcctc caagtaccgt
     1861 gaagtcttga gcatatgcat aaaccagtct tccatcaact gttccatacc ctgttactac
     1921 accttcgccg ggggtttcca cttcttccat cccgaaatct gtacatctgt gcttcacaaa
     1981 tagtccgatt tctctaaaac ttccttcatc aaacaatagg ttcaatcttt ccctagcagt
     2041 cagctttccc tgtgcatgct gcttttctat tcttttagga ccgccgcctt gttttaattt
     2101 cgatttccta ctgtatactt cttctgtttt attagacatt ttttacctcc aaagtattga
     2161 gcttagtgcc tgcaagacac tctcattctt ttgaactcct gttagaattg cttgattcta
     2221 taaaacttat gatacctctt tgatacgtat atcaatttct aatatattta tctcctcatc
     2281 tcacaatact attccggtta ttgataattt tcatcaagag tttgctttaa acgttattat
     2341 cgttttctac tgtcttaatc agtctctttg gcagagctct ataagaactc caaatgtaga
     2401 cttaggatgg cagaatgcta tcttagctcc tccggctccg tagcgcggct tctcgtctat
     2461 cattctgatt ccattttctt tcatttctgc tattgctttt tctatatcat caactctgta
     2521 agctatatgc tgaatacctt caccgttttt tgcaatatat cttgcaattg gtccgtcttc
     2581 ttctgttgac tccaaaagtt caacctcagt atctcctaat ggaaggaaag ctaccctaac
     2641 tttttgctct tccactgttt ctgttccggc tgattttaat ccaaggatat tttcgtagaa
     2701 atttaacgct tcatctaaat tttttacggc aatacctata tgatctactt ttttaaccat
     2761 aattacctcc ttcattaaat atcattgagt acttttaaaa attcatctct tacttcataa
     2821 acattcttat ttttattcaa tatttcaatt acttcactat ctaatctgtt ttcgctgtct
     2881 gtttgcgata taacaatttt tctaagctct tcttctataa gctccacaac ttctaatttt
     2941 aaattgttct ttcgtctttc ttcaaatttc ccgcttgctt tcaggtattt tatatgctcc
     3001 ttaatttcat tcaacaggtc atcaatacct tcattattta cagctacaac ctttactaca
     3061 ggaggtcttc tgtccatatc catggttaaa tcaagcatca tgtttacttc aacggctgtg
     3121 cggtcagctc caaacaaatc acttttattg attgcaaaaa tgtcgcctat ttccattatg
     3181 cctgccttaa ttgattgaat atcgtcacca agaccgggaa ccattaccat tacaattgta
     3241 tctgcaattt taactatatc gacttctgac tggccgacgc ccacggtttc gataattatg
     3301 taattgtagc cggctgcatc caataccttc aaagcattct ttgtatactt tgataaacct
     3361 cccaagtaac ctctggttcc catactgcgt atgaaaacat cggggttcag tgcaaggtct
     3421 tgcatacgga ttctgtctcc aagtatggag cctcctgaat aaggactggt tgggtctatt
     3481 gcaataaccc caaccttgtt tccttcactt accaatgctt taactatttt atccgtaagc
     3541 gtgcttttcc ctgcaccggg aggtccggta attccgatta tatgggcgtt tcctgtatat
     3601 tggtggattt ctcttataat tttttcagct ttttcgggct cgttttccac tgcagtaata
     3661 agcttggcac aagctctttt gtcacctata agcattttct ccaccaactg cttatactcc
     3721 attatgccac catccattct tatcggggac attcctcttg cctacaaaga cttactctat
     3781 agtagaggtg tgtcccctta cctttgtaca ttatttttta tatattcaat catttttgag
     3841 gtttctgtgc ccggtgtgaa tatttcctta attcctgctt ccttaagacc ttgaatatcg
     3901 tcatcgggaa ttactcctcc gcctattatc agtacatcat caacaccttg ttcctttaac
     3961 aactcggcaa ctcttggaaa taaatggtta tgtgctcccg a
//
LOCUS       DAOSOW010000151         3987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_22760_length_3987_cov_4.443540, whole genome shotgun sequence.
ACCESSION   DAOSOW010000151 DAOSOW010000000
VERSION     DAOSOW010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22760_length_3987_cov_4.443540"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /gene="prmC"
                     /locus_tag="PLD67_04880"
     CDS             <1..730
                     /gene="prmC"
                     /locus_tag="PLD67_04880"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOT21888.1"
                     /translation="LHLNRKEEVSEEIKDKFYKLAEKRNNGYPLQYITNSQEFMGLDF
                     YVQEGVLIPRPDTETLVETVINIVKERYNKEIKILDLGTGSGAIALSLAYYLRNSFVT
                     AIDISDIAVETTKINIKKHNLNNIKVIKGNIFEDILGEKFDVLVSNPPYIESDEIKNL
                     QTEVSVYEPRLALDGGVDGLDYYRPIVELFKKISEKNGVLAVEIGKQQKKSVEEIFLN
                     SKVFKEVKSYRDLAGNDRVIIGMT"
     gene            835..1905
                     /gene="prfA"
                     /locus_tag="PLD67_04885"
     CDS             835..1905
                     /gene="prfA"
                     /locus_tag="PLD67_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOT21889.1"
                     /translation="MLDKLEALKDKYTELSEKLSDPDILSDSQKLQKLMKEQAQIEPI
                     VLKYNEYKDIRNQLEESKEMLKEKLDDDFKEMVKEEIKELSSKVEELENEIKILLLPT
                     DPNDEKDVIVEIRAGVGGDEAGLFAGDLLRMYLRYAERRGWKTEFISVNEQGIGGYKE
                     VIFSIKGKGAYSRLKYESGVHRVQRVPDTESSGRIHTSSATVAVLPEADDIDIEINPN
                     DVKVDVFRSSGNGGQSVNTTDSAVRLTHIPTGIVISCQDEKSQLKNKDKAFKVLKARL
                     YDKYLQEQTDELAQSRRSQVGSGDRSERIRTYNFPQGRVSDHRIGLTLYKLDAFLDGD
                     ITEMIDALITSDQAEKLKNEMP"
     gene            1939..2184
                     /locus_tag="PLD67_04890"
     CDS             1939..2184
                     /locus_tag="PLD67_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOT21890.1"
                     /translation="MRIDKYLKSARIIKRRTIAKEACEQGRVLINGKIAKPGAEVEIG
                     DEIDIIFGTKPMKIKVEKLLEHTVKDEYKQMYSIINV"
     gene            2277..2813
                     /locus_tag="PLD67_04895"
     CDS             2277..2813
                     /locus_tag="PLD67_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOT21891.1"
                     /translation="MELKGSKTEQNLMTAFAGEAQAHVKYTYYQSQAKKDGYVQISKY
                     FEETARNEKEHAKLWFKLLHDGIAHTSVNLQDAADGEHYEWTEMYAEFAKVAREEGFD
                     KIAYLFEGVAAIEKSHEERYLALLNNIETGKVFKREGEVVWQCDNCGHIHVGSQAPKV
                     CPVCDHPQAHFQLYTKPY"
     gene            2955..3881
                     /gene="murB"
                     /locus_tag="PLD67_04900"
     CDS             2955..3881
                     /gene="murB"
                     /locus_tag="PLD67_04900"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOT21892.1"
                     /translation="MEYQLIINSMLDELAQSFRGLVLKDEPLKNHTYFKIGGPASIFA
                     VPEDTEDLKTVLKLIKKYEIDYYIIGNGTNLLVSDEGFKGIIIKIGEKFNKLSRDGNK
                     VTAGAGMLLSTLAKYFAKEGLEGFEFAGGIPGYLGGAIPINAGAYGGEMKDVVTKVKC
                     MDMDGRIHEFSNEEMEFEYRHSRLSDSEYIVLEAEISLTPGRVEDIMEKMKELNEKRI
                     SKQPLNLPSAGSTFKRPLNGYASKLIEDAGLKGLKYRGAMVSDKHSGFIVSYDNASCE
                     DVLELMRIVISTVYDKFGIILEPEIKIVGTKL"
BASE COUNT         1527 a          573 c          876 g         1011 t
ORIGIN      
        1 tcttcatctt aacagaaagg aagaagtaag cgaagaaatt aaagataagt tttataaatt
       61 ggctgaaaag aggaataatg gttacccgct gcaatatata actaattctc aggaatttat
      121 gggcttggac ttttacgttc aggagggtgt cctgataccg agacccgata ctgaaacatt
      181 ggtagaaacg gtaataaata tagtaaaaga aagatataat aaagagatta aaatacttga
      241 tttgggaaca ggcagcggag caattgcatt aagccttgct tattatttaa ggaactcgtt
      301 tgttaccgca atagacataa gcgatatagc tgttgaaacg acaaaaataa atataaaaaa
      361 acacaatcta aataatataa aggttataaa aggaaatatt tttgaagata ttttgggtga
      421 aaaatttgat gttcttgtat caaatcctcc atatatcgaa agtgatgaaa ttaaaaatct
      481 tcagacagag gtttcggtct atgagccaag gttagctttg gacggaggtg ttgacgggtt
      541 ggattattac agacctattg ttgaattatt taaaaaaatt agtgagaaga atggagtttt
      601 agctgtggaa ataggcaaac agcaaaaaaa atcagttgaa gaaatctttc tgaattctaa
      661 agtgtttaaa gaagttaaat cataccggga cttagccgga aatgacaggg taataatagg
      721 tatgacttag catcgcttat taattatgga gacacaccga agaatctcat gagatccttc
      781 gctgtgctcg gatgacataa caaacatatt agatttataa gagagaggaa taaaatgtta
      841 gataaattag aagcattaaa agataaatat acagaattaa gcgaaaaact atctgaccct
      901 gatatattaa gcgattcaca gaagctgcaa aagcttatga aagagcaggc gcagatagaa
      961 cctattgtgc ttaaatataa tgaatataag gatataagaa accagcttga agaatccaag
     1021 gaaatgctga aggaaaaatt agatgatgac tttaaggaaa tggtaaagga agaaattaag
     1081 gaattatctt ctaaagtaga agagctggaa aatgaaataa aaattctgct tctgccgact
     1141 gacccgaacg atgaaaagga tgtaattgtt gagataagag caggagtggg cggtgacgaa
     1201 gccggattat ttgcaggcga tttacttcgc atgtatttaa gatatgcaga aagaagagga
     1261 tggaagactg aattcatttc agtaaatgaa caaggaatag gcggttacaa ggaagtaatt
     1321 ttctctatta aaggaaaagg cgcatactca aggcttaaat atgaaagcgg tgttcacagg
     1381 gtgcagagag ttcccgatac agaatccagc ggcagaatcc acacctcatc tgcaaccgta
     1441 gcagttctgc ctgaagcaga tgatattgat atcgaaataa atccaaatga tgtaaaggtt
     1501 gatgtttttc gctcttcggg aaacggagga cagtctgtaa atacaactga ctctgccgta
     1561 agactaaccc acataccaac cggtattgtt atttcatgcc aggacgaaaa atctcagctt
     1621 aaaaataagg ataaagcttt caaggtttta aaggcgagac tttatgataa atatttgcaa
     1681 gagcaaactg atgaattggc tcaatcaaga agaagtcagg tgggctccgg agacagaagc
     1741 gagaggataa gaacttataa cttccctcag ggaagggtga gcgaccacag gataggtctt
     1801 actttgtata agcttgatgc atttcttgac ggagatataa ctgagatgat tgatgctctt
     1861 attacttcag accaagcgga gaaattaaaa aatgagatgc cctaacccta accagtccct
     1921 taaaaattgg aggttattat gaggatagat aagtatttaa aaagcgcacg cataattaaa
     1981 agaagaacca ttgccaaaga agcatgcgag cagggaagag ttcttattaa cggcaagata
     2041 gcaaagcccg gtgcggaagt agaaatcggc gatgaaatag atataatatt cggcacaaaa
     2101 ccgatgaaaa taaaggtaga aaaactatta gaacatacag tcaaagatga atacaaacaa
     2161 atgtacagca taataaatgt ttaatcagat tatcagggta tgagttaaaa ttggagagtt
     2221 tgatatctaa gcagccatgc tgctcacata agataaatat ttaaagaggt ggttatatgg
     2281 aattaaaagg gagtaagact gaacaaaatt tgatgactgc atttgcaggc gaagctcagg
     2341 ctcatgtgaa gtacacatat tatcaaagcc aagctaaaaa agacggttat gtacaaatca
     2401 gcaaatactt tgaagaaacg gcaagaaatg aaaaagagca tgcaaaatta tggtttaaac
     2461 ttcttcatga cggaatagca cacacaagcg taaacttaca ggatgcagca gacggtgaac
     2521 actacgaatg gacagaaatg tatgcagaat ttgcaaaagt ggcaagggaa gaaggattcg
     2581 ataaaattgc ctacttattt gaaggagttg cagcgataga aaaatctcat gaagaaagat
     2641 acttagcttt gttgaataat attgaaacag gcaaagtatt caaaagagaa ggggaagttg
     2701 tttggcagtg cgataactgc ggccatatcc atgttggctc ccaagccccc aaggtatgcc
     2761 cggtttgcga tcaccctcag gctcatttcc aattgtacac aaaaccctac taaaattaga
     2821 aaattgccat tcaatatggc aatttttttg ttctgataaa atataattac cattatgcca
     2881 tattaaaaat aaatctttaa aaataatcaa ttcaatgtta gaatatacat agttgatagg
     2941 gaaagggtgg aataatggaa tatcaattga taatcaacag catgcttgat gaattggcac
     3001 aaagtttcag gggtcttgtg ctgaaagatg agccgttaaa aaatcatact tacttcaaaa
     3061 tcggaggtcc tgcaagcata tttgcagtgc ctgaagacac agaagattta aaaacagttt
     3121 taaagctgat aaagaaatat gaaatagatt attatattat aggaaacgga acaaatctcc
     3181 tggtttcgga cgaggggttt aaggggataa taattaaaat aggggaaaag ttcaacaagc
     3241 ttagtcgtga tggaaataaa gtaacagcag gagcaggaat gcttttgtcc accttggcaa
     3301 agtattttgc aaaggaaggc ttggaaggtt ttgaatttgc aggaggaatc cccggatatt
     3361 tgggaggagc aattccaata aatgcaggag cttacggcgg ggaaatgaag gatgtggtaa
     3421 cgaaggtcaa atgcatggat atggacggca gaattcatga attttccaat gaagaaatgg
     3481 aatttgaata tagacattcc agactgtccg attcggaata cattgttctt gaagcagaga
     3541 taagtttaac acccggcaga gttgaagata taatggaaaa aatgaaggag cttaatgaaa
     3601 agaggatttc gaagcagcca ttaaatttac caagtgccgg aagcacattt aagagaccat
     3661 taaacggata tgcttccaaa ttaatagaag atgcaggact taaagggctg aaatacagag
     3721 gagcaatggt ttcagacaaa cacagcggct ttatcgttag ttatgataac gcatcttgtg
     3781 aagatgtcct ggagcttatg agaatagtaa taagtaccgt atatgacaaa tttggaataa
     3841 tattagaacc tgagataaaa atagtaggaa cgaagttgta gaagacaaag taaaacatat
     3901 aaaataacaa ggaagtttta aatgaaacta agagctgatt atcacataca ctcaacatat
     3961 tcaaaaaata ggcacggcaa atcatca
//
LOCUS       DAOSOW010000152         3968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_22893_length_3968_cov_3.270125, whole genome shotgun sequence.
ACCESSION   DAOSOW010000152 DAOSOW010000000
VERSION     DAOSOW010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22893_length_3968_cov_3.270125"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="PLD67_04905"
     CDS             <1..468
                     /locus_tag="PLD67_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21893.1"
                     /translation="MSGRGKDLKDTQGRDADYAYYIPNLRLWFNENLMYPFLGGDGIM
                     SENENITNLIPSINLLLPFYSPMYIRGASKEAIYNLSMACLENAKHILLALEKEYKEI
                     FERNLTVKRLGEVLLSPRLPYLGDNIYYDLNKEASGYMDVNIETLLKLERIIK"
     gene            468..1661
                     /locus_tag="PLD67_04910"
     CDS             468..1661
                     /locus_tag="PLD67_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HOT21894.1"
                     /translation="MFYYKYNDKYLFSIKDEYSFTKINSIPHDCQELYYLNNLTGNSK
                     RAFSVNHPSDLYINEESLDMLRVNDISYDLPENITELIDKRKIKAVNTAYSDYADCFY
                     MDEFNKRKVNILALGDVGSTLLIGLKLLGKDSISSIGIYDRSLDKIKRWEYEMNQTSF
                     PFDDSLPTVQGINKEELFDCDMFIFCASKGVPPVGSQVADVRMAQFEGNAELIKEYAL
                     MAADCRFKGIFAVVSDPVDQLCQTVLKESRGVLAPEQIKGYGLGVMNARALYYSKKFN
                     KFSMYLKEGRAFGPHGKDLIIANSIRDYDDELSRELTKYAVEANLEVRKTGFKPYIAP
                     ALSSGAISIIYTLEGKYHYSSNYLAGVYMGAKNRNLPSGLDIEKIQMPDKLFERIKKT
                     YNKLK"
     gene            1806..2852
                     /locus_tag="PLD67_04915"
     CDS             1806..2852
                     /locus_tag="PLD67_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006305832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOT21895.1"
                     /translation="MNRLTVLKFGTSERLEKMLASSLNEYEYDLITNVDDMNDLQNKK
                     ILFAIELGDTGINIEHMKMLNKIRLSGPDFMKDSIGCILINSLSELFSRAEARRTIFY
                     ANMAGCLFPGKPLSEATGSLRNFNTRQTITEGDVSLEDMLLLDSREVVERVMNYTAKK
                     ITAPNMLTLYSGNPKTSNTYALWSMVKENLENYHITEIHVENGSVADCRGCPYKTCKH
                     YSQSTNCFYGGIMVEEVYPAILDCDVLMMLCPNYNDSISANMSAVINRLTALYRKTKF
                     YDKHFYSIIVSGFSGSDLLAQQLISALNINKTFMLPPKFALMETANNPGDVKKIENIE
                     KKAAQFAKNIMSLL"
     gene            complement(2940..3013)
                     /locus_tag="PLD67_04920"
     tRNA            complement(2940..3013)
                     /locus_tag="PLD67_04920"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2977..2979),aa:Pro,seq:ggg)
     gene            3163..3558
                     /locus_tag="PLD67_04925"
     CDS             3163..3558
                     /locus_tag="PLD67_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOT21896.1"
                     /translation="MNDKELFDCGCADENCDCNYDEFDDDCECGCGCGDHEEEIMTIT
                     LEDGREVDCAIIATFPVEGKDYIALLPLDGNEEEGEVFLYEFKEIEDGIELIGIESDE
                     EYELVADAFDEILEEADLEELFDDDEDED"
BASE COUNT         1386 a          630 c          799 g         1153 t
ORIGIN      
        1 atgtccggca ggggaaagga tttgaaggat acacaaggaa gagatgcaga ctatgcttat
       61 tatatcccta acttaaggct ttggtttaat gaaaatttga tgtatccttt tttgggagga
      121 gacggcataa tgagtgaaaa tgagaatata acaaatttga ttccttcaat aaatttattg
      181 ctgccctttt attcgcccat gtatataagg ggagcttcca aagaagcaat atacaatctt
      241 tctatggcct gcctcgaaaa tgcaaaacac attcttcttg cattggaaaa ggagtataag
      301 gaaatctttg aaaggaacct gactgttaaa aggctgggtg aagttctttt gtcaccgaga
      361 ctgccctacc tgggagacaa tatctattac gatttaaaca aggaagcatc cggatatatg
      421 gatgtaaata ttgaaacact tttaaaactt gaaaggatta taaaataatg ttctattaca
      481 aatacaatga taaatattta ttctcaataa aagatgagta tagttttaca aaaataaata
      541 gcattcctca tgactgccaa gaattatatt atcttaataa cttaaccgga aattcaaaac
      601 gcgctttttc cgttaaccat ccttccgact tgtacataaa tgaggaaagc ctggatatgc
      661 ttagagtaaa tgacataagt tatgaccttc ccgaaaatat tactgagctg atagacaaaa
      721 gaaaaattaa ggcagtcaac accgcctaca gtgattatgc tgattgcttc tatatggatg
      781 aatttaataa aaggaaggta aatattcttg ccctcggtga tgttggaagt acattactta
      841 ttggacttaa acttctcgga aaagattcaa taagctccat cggtatttat gacagaagtt
      901 tggataaaat aaaaagatgg gaatatgaaa tgaatcaaac atcatttcct tttgatgatt
      961 cgctgcccac ggttcagggt ataaacaagg aagaattatt tgattgtgac atgtttatat
     1021 tttgtgcttc caagggggtt cctcccgtag gaagccaagt tgccgatgtt aggatggctc
     1081 aatttgaagg aaatgcagag cttattaagg aatatgcctt aatggcggca gattgccggt
     1141 ttaagggaat atttgcagta gtttcggacc ctgtagacca attatgccaa acagttctaa
     1201 aagagagcag gggtgttttg gcacccgagc aaattaaggg gtacgggctt ggagtaatga
     1261 acgcgagagc tttgtactac tcgaaaaaat tcaataaatt ttcaatgtat ttaaaagaag
     1321 gaagagcatt cggaccgcac gggaaagact tgataatagc aaacagtata agagattatg
     1381 atgatgagct ttcaagagaa ttgacaaaat atgcggttga agcaaacttg gaagtaagaa
     1441 agacgggatt taagccatat attgcacctg ccctttcatc cggagcaatt tcaataattt
     1501 atactctcga agggaaatat cattacagct ccaattattt agccggagtt tacatgggag
     1561 ctaaaaacag aaacctgcct tcaggactgg atatcgagaa aattcaaatg cccgataagc
     1621 tttttgaaag aattaaaaag acttataaca aattaaaatg acatgagtgt cattctgaac
     1681 gcagtgaaga atctcacttt cagagcgcag cccccgtcat tctgaaccca gtgaagaatc
     1741 ccactttcag agcgcagccc tcgtcattct gaacgaagtg aagaatctca tttttaggtg
     1801 atattatgaa tagattaact gttttaaaat tcggaacatc ggaaagactg gagaagatgc
     1861 ttgcatcttc tttaaatgaa tatgaatacg acctcataac aaatgttgat gatatgaatg
     1921 atttgcagaa taaaaaaata ttatttgcaa ttgagctggg ggatacggga attaatatcg
     1981 agcatatgaa aatgcttaat aaaatcaggt tatcaggacc tgattttatg aaagactcca
     2041 taggttgcat attgataaat tctctaagtg agttgttctc aagggcagaa gcaagaagaa
     2101 caatttttta tgccaatatg gcaggatgtt tgtttccggg aaagcccctg tcggaagcca
     2161 caggctcctt gagaaatttc aacaccaggc agaccattac tgaaggagac gtatcattgg
     2221 aagatatgct cttgttggat tctcgggaag tagtagaaag ggtgatgaat tataccgcaa
     2281 aaaaaattac cgctcctaat atgttgacac tttattccgg taatcccaaa acatcaaata
     2341 catatgctct ttggtcgatg gtaaaagaaa atttagaaaa ctaccatatt actgaaattc
     2401 acgtagaaaa cggctccgtt gcagactgca ggggatgtcc ctacaagaca tgtaaacact
     2461 attctcaaag cacaaactgc ttttacggag gaataatggt tgaagaagtt tatcctgcaa
     2521 tacttgattg cgatgtattg atgatgttat gcccaaacta caacgattca attagtgcaa
     2581 acatgtcggc agttataaat cgcttaacag ctttatatcg aaaaacaaag ttttacgata
     2641 aacattttta ttccataatt gtatcgggat tcagcggaag cgacctttta gctcagcagc
     2701 tgataagtgc tttgaatatt aacaaaacat tcatgctgcc gccaaaattt gctttgatgg
     2761 aaactgcaaa taatcccggc gacgttaaaa aaatagaaaa tatagaaaaa aaagcggcac
     2821 aatttgcaaa aaacatcatg tcattgctat agtgacaagg ggacgtgaca aggggacggg
     2881 ggtagtgtca tccagtaatg aaacaataaa aaagggaaca cttagttccc ttttttttat
     2941 cgggatgaca ggatttgaac ctgcggcccc taacacccca tgctagtgcg ctaccaaact
     3001 gcgccacatc ccgacaagca tataaaatta taacggaaaa atcagcttct gtaaagagga
     3061 aatttttata aaaattatat attgcatttt atattccatt ctgttaaagt ttatgtatct
     3121 atccatggtt atggataaat aaattataag gatgtaaaaa atatgaatga taaagaatta
     3181 tttgattgcg gctgtgcaga tgaaaattgt gactgcaatt atgatgaatt tgatgacgat
     3241 tgtgaatgcg gctgcggatg cggagaccat gaagaagaaa ttatgacaat tacattggaa
     3301 gacggaagag aggtcgattg tgcaattatt gcaacattcc ctgttgaagg aaaagattat
     3361 attgctttgc ttccattgga tggtaatgaa gaagaaggtg aagtttttct ttatgaattt
     3421 aaagaaatcg aagatggaat tgaattgata ggtatagaat cagacgaaga atatgaatta
     3481 gtggcagatg catttgatga aattctcgaa gaagcggatt tagaagaatt atttgatgat
     3541 gacgaggatg aagattaata tacataaaaa aaaaggaact gttgatacaa cagttccttt
     3601 tttaaattat attgtccaat acagagcctc ttgctcctgc tgttgtcctt gcaagcttta
     3661 tctaaggttt caggactgca ctaatgtcaa aaaatgcaac agcgtgtcaa agcattgtaa
     3721 aatagtgtgc tatatgatat aatttaataa tctaaggata aatcttaaac caaatgagga
     3781 aagcttatgc taaaacggca attgtacata ttgttaatac tcatattatt atttacatat
     3841 caaattatct tcgtagaaac cgtggttcat gccgatgttg ggactatccg agttgcattc
     3901 aattcaaatt taaagcccta tcattttatt aacaaattct ggaagtgctt ctttaattat
     3961 tgctttaa
//
LOCUS       DAOSOW010000153         3940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_23097_length_3940_cov_2.679022, whole genome shotgun sequence.
ACCESSION   DAOSOW010000153 DAOSOW010000000
VERSION     DAOSOW010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23097_length_3940_cov_2.679022"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="PLD67_04930"
     CDS             complement(<1..501)
                     /locus_tag="PLD67_04930"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT21897.1"
                     /translation="MELVNEPKTKRGMETLDRICIAAEELFSEKGYYDTGITDIVTRA
                     GIAQGTFYIYFNDKKSVFVYLIRKLGSELKESIRANIRVSKDRYDEEYMGIKTFLNFV
                     GEHIGLFKIVWQAQFVDPELFKEYYESFSKGYVKNIKKAQTKGEMNDIDPVILSYCLI
                     GIANFTA"
     assembly_gap    644..653
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(676..1341)
                     /locus_tag="PLD67_04935"
     CDS             complement(676..1341)
                     /locus_tag="PLD67_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOT21898.1"
                     /translation="MNDKQMQSNYIKIVGSIFSNLEYSHEVFGEKFYEFFIEVPRLSE
                     TKDILPVIISERIINDINMDIGNHIVIEGQFRSYNRYEDDSNRLLLRIFVRDIYVPDE
                     TELEEMKRHPNEVFLNGYLCKETKFRTTPFGREITDMLIAVNRSYNKSDYIPCIAWGR
                     NARYCEKLEVGDHIKLWGRIQSRKYQKKNEHDIYETKTAYEVSITKLEHIKEENNRKK
                     DDE"
     gene            1599..2690
                     /gene="ychF"
                     /locus_tag="PLD67_04940"
     CDS             1599..2690
                     /gene="ychF"
                     /locus_tag="PLD67_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOT21899.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITSAGADSANYPFCTIEPNIGVVEV
                     PDKRLNFLTKMSNSGKTVPAVIEFFDIAGLVKGASKGEGLGNKFLSHIREVEAVVHVV
                     RCFEDENVIHVETTVDPKRDIEIIELELVLADLEVMSKRLDKLSKMVKNDKSLQAEFE
                     LVQKIVQGLENEKQARYLSYTDEEQKLLKTFNLITSKPVLYVANVDESYLSDPSGNKY
                     VDIVKEYASRDNSEVIVICGKIEEEISVLDEDEKKEFLEEMGLKESGLDKLIRASYRL
                     LGLISFFTTGPMESKAWTIVKGTKAPQAAGKIHSDMERGFIRAEVTKYDDVVKLGSMH
                     AAKEKGLMRLEGKDYVMEDGDIVLFRFNV"
     gene            complement(2786..3463)
                     /locus_tag="PLD67_04945"
     CDS             complement(2786..3463)
                     /locus_tag="PLD67_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOT21900.1"
                     /translation="MAILERFGDIIKANINVLLEKMEDPEKMIDQYLEDMMKDLAEVK
                     QSTAEVIAEETRTKRLVEQNEAEVAKYNEFAKKALVAGNEDDARTFINKKQELENAGA
                     GLMTAYAAAHENAIKMRQMHDKLTADIETLRARRQVIKSKVKVAKTQETLNEATQATK
                     KIAGAKSAFERMEDKANRMLDEANAMAELNMEPIDEAKALEEKYAKMGNASVDDEISK
                     LKEELGL"
     gene            complement(3502..>3940)
                     /locus_tag="PLD67_04950"
     CDS             complement(3502..>3940)
                     /locus_tag="PLD67_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21901.1"
                     /translation="YDELFTDEAHLLIVFFEYEGQYKGWYIVGTQAKSVIDAEAGNIL
                     ADYIDRYYYDSSLSNEEFFSKAFTDAADRIMTVTRSPWITVFIVLGVVVLVIVLFIWW
                     KKHKEQKNLEAKRREEMLNTPLERFGTTEAEDLMKKYQDDNEQ"
BASE COUNT         1191 a          720 c          591 g         1428 t
ORIGIN      
        1 tgccgtaaaa tttgcaatac caattaaaca atacgataaa ataaccgggt caatatcatt
       61 catttctcct ttagtttgcg cctttttaat atttttgaca tatccttttg aaaatgattc
      121 ataatattct ttgaataatt caggatcaac aaattgagcc tgccatacga ttttaaaaag
      181 accaatgtgt tcacctacaa aatttaagaa tgttttaatt cccatatact cttcatcgta
      241 cctgtctttg ctcactctta tgttggctct aatactttct ttcaattcac tgcccagttt
      301 tcttatcaga taaacgaata cacttttttt atcgttaaaa tatatgtaaa atgtcccttg
      361 agcaataccg gctctcgtaa caatatctgt aataccggta tcataataac ctttttcgct
      421 gaataattct tcagcagcta tacatattct atctaatgtt tccattccac gcttcgtttt
      481 aggttcattg actaattcca tattttcacc tgcttataat cgttattaca ccattaattc
      541 aaattataga cgatttgcac ataaaaatac aactattttt taattatttt tttaacattt
      601 aaataatata gtgcaaaaaa attatgatat aatgtaaaaa acgnnnnnnn nnntggcagt
      661 cgttttgtgt tttaattatt catcatcttt ttttctgtta ttctcttcct ttatatgttc
      721 aagctttgtt attgaaactt cataggctgt ctttgtttca tagatatcat gctcattttt
      781 cttttggtat ttcctgcttt gtattcttcc ccaaagttta atatgatctc caacctccaa
      841 cttttcacaa taccttgcat tcctccccca tgcaatgcaa ggtatgtaat cggacttatt
      901 atatgaacga ttaactgcaa tcagcatatc agtaatttcc ctgccgaacg gagtagtcct
      961 gaatttagtt tctttgcaaa gatatccgtt taaaaaaact tcgtttggat gccttttcat
     1021 ttcttcaagt tcagtttcat caggtacata tatatctctt acaaatattc ttaacaaaag
     1081 cctgtttgaa tcatcctcat atcggttgta tgacctgaac tgaccttcaa ttactatgtg
     1141 atttccaatg tccatattta tatcattaat tatcctttcg gatataataa caggcagtat
     1201 atccttggtt tcacttaatc ttggaacttc tataaaaaat tcgtaaaatt tttcaccaaa
     1261 tacttcatga ctgtactcca aattgctgaa tatgctcccg actattttta tataattgga
     1321 ctgcatctgt ttatcattca tataactttt tccccctata atttgtctta tatattttat
     1381 taatattcat tgcaataaaa tatatgcttt aaaagccaaa aagttgtcta ttttttagta
     1441 ttcctaatgc aaatttattt aatgttgaac tttaaaattt aatattatat aatgaatatt
     1501 attaaaaatc agtttgcgta acaggataac aaataagaac atattcattt attgtaagta
     1561 ctaacgtgta aatttgaaga aaggatggtc acaccatcat gaaattaggt atagtaggac
     1621 ttcctaatgt aggcaaaagc accttgttta atgcaattac ttcagcaggt gcagattcgg
     1681 ctaattatcc cttttgcacg atagagccta atatcggagt agttgaagtt cccgataaaa
     1741 gattaaattt tttaactaaa atgagcaatt cagggaagac agttcctgca gttatagaat
     1801 tctttgatat tgcaggcttg gtaaaagggg caagcaaagg agaaggatta ggaaataaat
     1861 ttctttcgca tataagagag gtcgaagctg ttgttcatgt agtgagatgc tttgaagacg
     1921 aaaatgtaat acatgttgaa actacggttg accctaaaag agatatagaa ataatagaat
     1981 tagaattggt tttagctgat ttggaagtaa tgtcaaaacg acttgataag ctttcaaaga
     2041 tggttaaaaa tgataagtcc ctgcaggcag aatttgaatt ggttcaaaaa attgttcaag
     2101 ggcttgaaaa tgaaaaacag gcacgttacc taagctatac tgacgaggaa caaaagcttt
     2161 taaaaacttt taatttgata acctcaaaac cggttttgta cgtagctaat gttgatgaaa
     2221 gctatctttc agacccttca ggcaataaat acgtggatat agtaaaggag tatgcttctc
     2281 gggataattc ggaggtaatt gttatttgcg gaaaaataga agaagaaata tcggttttag
     2341 atgaggatga aaagaaagaa tttttagagg aaatgggact aaaagagtca gggcttgata
     2401 aacttattag agcaagctac cgcttacttg gacttataag cttttttacg acaggaccaa
     2461 tggaatcaaa ggcttggaca attgtaaaag gtaccaaagc tccacaagct gcaggcaaaa
     2521 tacactcgga catggaaaga ggtttcatac gtgcagaagt cacaaaatac gatgatgttg
     2581 taaaattagg ctcaatgcat gcggcaaagg aaaaaggatt gatgcgtctt gaaggtaaag
     2641 attatgtaat ggaagacgga gatatagtat tattcagatt caacgtttaa ttgtcatttt
     2701 tctttactag agagaaatac ataattattt gttgtcttat atttgaaatt actaaaaagc
     2761 tgcctattcg gcagcttttt ggttattaca atcctaactc ttcttttaac ttacttattt
     2821 catcgtctac tgatgcattg cccatttttg catatttttc ttctagagct tttgcctcat
     2881 ctatgggctc catattcagc tctgccatgg catttgcttc gtctaacatc ctgtttgctt
     2941 tgtcttccat acgctcaaaa gcactttttg caccggctat tttctttgtt gcctgagttg
     3001 cttcatttag tgtttcctgt gttttagcta ctttaacttt tgatttgata acttgtcgcc
     3061 tagctcttaa agtttcaatg tctgcagtta acttatcatg catctgtcgc attttaattg
     3121 cattttcatg agcagccgca taagcagtca ttagtcctgc tcccgcattt tccagttctt
     3181 gttttttgtt aataaatgtt ctagcatcat cctcattacc ggcaactaag gctttcttgg
     3241 caaattcgtt atacttcgca acttctgcct cattttgctc aactaatctc tttgtacgtg
     3301 tttcttcagc tataacttct gccgtgcttt gttttacctc tgcaagatct ttcatcatat
     3361 cttccaagta ctggtcaatc attttttccg ggtcttccat tttttccagc aatacattta
     3421 tgtttgcctt gataatatct ccaaatcttt ctaatatagc catttcttcc tccttttaat
     3481 ttaaccctgc cttttggcag attattgttc attatcatcc tgatattttt tcatcaggtc
     3541 ttcagcttct gttgtaccaa acctttcaag aggggtattt agcatttctt cccttctttt
     3601 tgcttccaaa tttttttgct ctttatgctt tttccaccat atgaaaagta caataactaa
     3661 aacaactaca cctaatacaa taaacaccgt aatccatggc gacctggtta ccgtcattat
     3721 tctgtcagct gcatcagtga aagctttgct gaaaaattct tcattgctca aactgctatc
     3781 ataataatat ctgtctatat aatcagccaa aatattaccg gcttcagcat caattacact
     3841 ttttgcctgt gttccgacga tgtaccagcc tttgtactga ccttcatatt caaaaaatac
     3901 tataagtaaa tgtgcttcat cagtaaacaa ctcatcatat
//
LOCUS       DAOSOW010000154         3939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_23102_length_3939_cov_4.276004, whole genome shotgun sequence.
ACCESSION   DAOSOW010000154 DAOSOW010000000
VERSION     DAOSOW010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23102_length_3939_cov_4.276004"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(108..797)
                     /locus_tag="PLD67_04955"
     CDS             complement(108..797)
                     /locus_tag="PLD67_04955"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOT21902.1"
                     /translation="MEDVIKEIKNNSLFFNMSEDEIKFVLKCSNSTVKDLRDNYALFE
                     KDQKVKKLGIVIDGILNLESRKYNGNRVIVTTLEKNDLFGEALIYASEGSSPYDLVSS
                     GNSKVLLIPYKIFSNMCFNNCSFHAQLIKNMLTILSDKIVMLNNKMNILNAETLKGRI
                     AVYLLSLQKKSNSMIFNMPMNRQELAEFLNVKRPSLSRELSNMQKEKIIEVYRSSVKI
                     LDMDKLCELAD"
     gene            complement(923..1699)
                     /locus_tag="PLD67_04960"
     CDS             complement(923..1699)
                     /locus_tag="PLD67_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005885749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT21903.1"
                     /translation="MLDIINLSKTFNQGTDYEISIFSNFNIHINKGECTAILGANGCG
                     KSTLFNIISGFLMQEKGIIKLNGKNINGLKENERAKFIGRVHQNPSAGVSPSLTILEN
                     ISLSDKKCQKFTLKKLIRKNKIEAYKEILRTLDLGLETKLDTEVKYLSGGQRQSLSLL
                     MATMNKPQLLLLDEHTAALDPKTSRLVMEKTQELIKTQNITTMMISHNARDALMYSDR
                     IIMLDKGEIILDLRKGQITEKELLNIYNSRNYGSPLTEAV"
     gene            complement(1693..2571)
                     /locus_tag="PLD67_04965"
     CDS             complement(1693..2571)
                     /locus_tag="PLD67_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT21904.1"
                     /translation="MNTLILTSIEQGLIFSVLAIGVIITFKILDFADMSVEGTFPMGA
                     FIFAKFITTGFSPIISTLLTFILGTLAGLLTYTLNIKLRIRALLSGILTATILYSLNL
                     RLNGRSNIGLTSYDSIFDGFRPVIVLILIVLAVKILMDLFLKTEVGYLLIATGDNETL
                     VKSLGENSNKYKLIGLMLSNGLVSLSGALMAQYQGFSEISMGTGIIVVAIASIIVGDT
                     VLRNSKILKMTTRAIMGALIYKFIGALAIDLGLAPTDLKAINAAIVIIFLSYNTFSPV
                     KMRKARKIKAVEGVKC"
     gene            complement(2568..3554)
                     /locus_tag="PLD67_04970"
     CDS             complement(2568..3554)
                     /locus_tag="PLD67_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT21905.1"
                     /translation="MKNSTIKFIAILFLVSLIITGCSANTEANKPAGGKFTIGIAQLV
                     QHPALDASRQGFIDEMEKLGVEVEFIDQNAQADINNAQMIAEKFVKDDVDLIFSIATL
                     TSQAAKKAIEGTKIPLVFTAVTDPVFSELVTALDVTDNNITGVVDAAPIKENLELFKL
                     LKEDIKTVGIIYNIGESNSEVQVEQTKEIAAELGLTIETVGITSINDIPQAVDTISKK
                     VEGLYIITDNMVASAMELVAKLAGEKDLLTVSADGSHVDQGILISKGISYYVIGQQSA
                     RIAKQILVDKVDVADIPVQASTEFEKKVNLKTAESLGLAKEHKAFEGAEFVE"
     misc_binding    complement(3685..3914)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         1123 a          783 c          600 g         1433 t
ORIGIN      
        1 cctgagatcc ttcgcttacg ctcaggatga caatcgcaga gcatattcgt ttatcgtggt
       61 gtagtatacg tgatgataaa gagataacag tttaaaaaaa gtaataatta gtctgctaat
      121 tcacagagtt tatccatgtc caatatttta acggaagagc ggtaaacttc aataattttt
      181 tccttttgca tgttggaaag ctcccttgac agagatggtc ttttaacatt taaaaactcg
      241 gcaagttctt gtctgttcat gggcatgtta aatatcatgg aatttgattt tttttgaaga
      301 gacaaaagat atactgcaat tctgcccttt aatgtttcgg catttagaat attcatttta
      361 ttattgagca taactatttt atcggataaa atggtaagca tgttttttat caattgagca
      421 tggaagctgc agttgttaaa gcacatattg gaaaagattt tatagggtat tagcaatact
      481 ttgctgtttc ctgaactcac aaggtcatag ggagaactac cttcggaagc atagattaaa
      541 gcttctccga ataggtcatt tttctccaat gttgttacta taaccctgtt gccgttatat
      601 tttcgggatt ccagatttaa tattccatca attacaattc caagcttctt tactttttgg
      661 tccttttcaa ataaagcata attgtccctt aaatctttaa cggttgaatt actgcattta
      721 agaacaaatt ttatttcatc ctcgctcata ttaaaaaaca gcgaattatt tttaatttct
      781 ttaataacat cttccataat aaaacctttc ttcatctgtc tttgatgaat aactcgtggt
      841 cgtcaataac ataattatac aatactatca tttaggtgtc aaaaacaata aaaaggaagg
      901 ataattatta tccttccctt agctatactg cttccgtcag cgggcttccg taatttctag
      961 agttataaat attcagtaac tccttctccg ttatctgccc ctttcttaga tcaagaatta
     1021 tttctccctt gtcaagcata ataattctat ccgaatacat tagggcatct cttgcattgt
     1081 gagatatcat catagttgta atattttgag tttttattaa ttcctgagtt ttttccatta
     1141 caagccttga ggttttgggg tctaaggctg ctgtgtgctc atccaacaaa agaagctgcg
     1201 gtttattcat tgttgccatc agcaaggaca gtgactgcct ttgccctcct gaaaggtatt
     1261 tcacttccgt atccaatttg gtttcaagtc ctaagtcaag agttcttaaa atttctttat
     1321 aagcttcgat tttatttttc ctgattaatt tttttaatgt aaatttctga cacttcttat
     1381 ccgacaggct tatattttcc aatattgtaa gtgacggcga aacgcctgcc gatggattct
     1441 gatgcactct tcctataaac ttagcccttt cgttttcttt taaaccgttg atatttttcc
     1501 cgtttaattt tataatacct ttctcctgca ttaaaaagcc gcttattatg ttaaacaagg
     1561 tgcttttccc gcatccgttt gctcccaata ttgccgtgca ttctcccttg tttatatgaa
     1621 tgttgaaatt actgaatata cttatctcat aatctgttcc ttggttgaaa gtcttcgata
     1681 aatttataat atctaacatt ttactccctc cacggctttt attttccttg cctttctcat
     1741 tttcacaggg ctgaacgtat tgtagctcaa aaatataatt actattgccg catttattgc
     1801 ttttaggtct gtcggtgcaa ggcctaaatc aatcgctaag gctccaatga atttatatat
     1861 taaggctccc ataattgccc ttgtcgtcat ctttaatatt ttgctgtttc ttaatactgt
     1921 atcgcccaca attattgaag ctattgccac cactattatc ccggttccca ttgatatttc
     1981 cgaaaaaccc tggtattgtg ccatcagagc tccgcttaac gatactaatc cgtttgaaag
     2041 cattaatcct atcaatttgt atttgtttga attctctccc aaggatttaa ccaatgtttc
     2101 attgtctcct gttgctatta acaaataccc cacttcggtt tttaaaaata aatccattaa
     2161 tatctttacg gcaagaacta tcagtataag cactattacg ggtcggaatc cgtcaaatat
     2221 tgaatcatag cttgttagcc ctatatttga tctgccgttt aatcttaagt ttagtgaata
     2281 taatatggta gctgtaagaa tccccgataa caaagctctt attcttagtt taatgtttaa
     2341 tgtgtatgtt aacagtcctg ccaaagttcc caggataaag gttaaaagtg tgcttataat
     2401 aggagagaag cctgtggtaa tgaatttagc aaatataaat gctcccatcg ggaatgttcc
     2461 ttcaacggac atatctgcaa aatcaagtat tttaaatgta ataataactc ctattgctaa
     2521 tacggaaaaa attaaacctt gttcaattga tgtaagaatt aatgtattca ttcaacaaat
     2581 tctgctcctt caaatgcttt gtgttctttt gccagcccca gactttctgc tgtttttaaa
     2641 ttaacttttt tctcgaattc ggttgatgct tgaacaggaa tgtcagcaac atcaacttta
     2701 tcaaccaata tttgtttagc tatccttgca gattgctgac ctattacgta gtagcttata
     2761 ccctttgaaa tcagtatccc ctggtctaca tgactgccat cggcggatac agtcaacaaa
     2821 tctttttctc cggctagctt ggcaactaat tccatagctg atgccaccat gttatccgta
     2881 attatatata gaccttccac cttttttgaa atggtgtcaa ctgcttgcgg tatgtcatta
     2941 attgaagtaa tcccgactgt ttcgattgtc agtccaagtt ctgctgcaat ttcttttgtc
     3001 tgttcaactt gaacttcaga gtttgattct ccaatattgt aaattatccc cactgtcttt
     3061 atatcttcct ttaacagttt aaacaactca aggttttcct ttatgggagc tgcatcaact
     3121 actccagtaa tattgttgtc tgtaacatcc aaagctgtta ccagttcaga gaaaacaggg
     3181 tctgttacag cagtaaatac caatggtatc tttgttcctt caattgcttt ttttgctgcc
     3241 tgagacgtaa gtgttgcaat cgaaaatatt aaatcgacat catctttaac aaatttttct
     3301 gcaatcattt gtgcattgtt tatgtctgcc tgagcattct ggtcaataaa ttcaacttct
     3361 acacccaact tttccatctc atctataaat ccctgtcttg aagcatccaa ggcaggatgt
     3421 tgcacaagct gtgctattcc tattgtaaat tttcctcctg caggcttgtt tgcttctgta
     3481 tttgcagaac atccggttat tatcagcgaa accagaaata aaattgctat aaatttaatt
     3541 gtactatttt tcataatgtc cctccaaggt ttatttattt ttagatatga acttggccag
     3601 ggttcgatat cgaaaaaaca tgattacagg taaatcataa aaaaaatctt tcattcctat
     3661 aaacatactt ttttaaaata tgcttatagg gacgaaagat ttccgcggta ccaccctaat
     3721 tatgatataa atcatcactc ttcgaagtct aacaacttct aaccctgtaa cgtgagtaca
     3781 cgtgttcgcc tactcggagt tccttttagc aaacctgctc agcagtgtat attatattcc
     3841 tctgttactg gctctcacca ccgccaattc tctgtaaacg tctgaaatac ttttctctgc
     3901 ttcaacgctt taaatattaa tagcaataat aacttataa
//
LOCUS       DAOSOW010000155         3934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_23136_length_3934_cov_3.596288, whole genome shotgun sequence.
ACCESSION   DAOSOW010000155 DAOSOW010000000
VERSION     DAOSOW010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23136_length_3934_cov_3.596288"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PLD67_04975"
     CDS             complement(<1..351)
                     /locus_tag="PLD67_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21906.1"
                     /translation="MKNKIWQKVMMFSISLILGIILVTQMRTKSNILGTVNLDKKAVE
                     LNEELAKLSIEKNRLRDELESLKKESRESEELFKDAEEELLKLKEELKVQKIISGYYD
                     VKGPGTIITIDAEPD"
     gene            complement(362..1144)
                     /locus_tag="PLD67_04980"
     CDS             complement(362..1144)
                     /locus_tag="PLD67_04980"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOT21907.1"
                     /translation="MDENNNKKTQRKKKRRRNKKLMVSTAAFITAAVVVVFCICYVYY
                     KTPYFNLIGVDIDGNLTYSDEYIMEKSGIEVGKKIFEVDRSKVKENIENEVYVENARV
                     VYELPNKIFLDIKERVNTYQVFFNNEYIIIDKNGIVLRTCNEKLDLLTIESYTDVVYN
                     IGDNIQFAGVENNEKVFNAIEYLNNEYGSDIIKAFNIELNNSFLFKTQYGTAVRIDLS
                     EDINYQIVFGMKIINERLNNNLTVATGLIDFTKGDSPVYIED"
     gene            complement(1358..2617)
                     /gene="murA"
                     /locus_tag="PLD67_04985"
     CDS             complement(1358..2617)
                     /gene="murA"
                     /locus_tag="PLD67_04985"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOT21908.1"
                     /translation="MGSFIVEGRNSLEGIVDIGGSKNAALPILASTVITGREYILENI
                     PDIEDVKDMLEILSNMGCSVQFENGIALIDTRGLNTHIVPEKLVRKIRSSIILMGAVL
                     QRTGKVEVAYPGGCEIGLRPIDLHLKGLRKLGVEITEKNGLLVGSVNEALSCDIHLDY
                     PSVGATENIMLFAVGNPGKTTIYNPAREPEITDLQNFLNTCGFCVSGAGTGIITIEGK
                     KVQNTDTVEYKIISDRIEAGTYLSAAASMKSKIYIRNIEKSHFKSITSLYEDAGCEIR
                     ETHGTIVIANNKRIKAIEKITTQPYPGFPTDMQAQVMASMTVADGTSIIEETVFDSRL
                     KHVPELQKMGANITTIGRVAVVEGVEKLYSAEVEAKDLRGGAALVIAAMVAEGTSKIN
                     NIHHIKRGYEKFIYKLQNLGVIITEIE"
     gene            complement(2677..3792)
                     /gene="murG"
                     /locus_tag="PLD67_04990"
     CDS             complement(2677..3792)
                     /gene="murG"
                     /locus_tag="PLD67_04990"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOT21909.1"
                     /translation="MRVLITGGGTGGHINPALAIAQKVKNENPSDHVLYVGSKNSLES
                     ELVPKEGFEFKAVTAKYFKRKFTLDNIKTLFASAKGIIESVKIINEFKPNIVVGTGGY
                     VSGPVVFAAHLKRIPTMILEQNVFPGITNRLLSRFADVVGISFEEAKKYFPQNAQEKL
                     IMVGNPVRKDIITIDRRKSRTRYNLKPDDVFIYSFGGSGGQISLNEAIIDVIKKYNGK
                     QNVKILHVTGKMHYDAFMEELNRNIKLEKNIEIKDYMYEAPSALSASDVVIGSAGAIT
                     IAEITAIGVPSILIPKTYTAENHQEYNARALEKEGAALVILEKDLNGRTLLKSIEEVI
                     ENKSRLNTMALNSKRLGNFDVENKIYATMMALINAHK"
BASE COUNT         1053 a          831 c          539 g         1511 t
ORIGIN      
        1 atccggctcg gcatctatgg ttattatggt tccgggtcct ttaacatcat aatagcctga
       61 tattatcttc tgaactttaa gttcttcttt aagctttaat agttcttctt cagcatcctt
      121 gaataattct tcactttctc tgctttcttt tttaagtgat tcaagttcat ccctaagtct
      181 gtttttttcg atgcttagct tagccagttc ctcattcagc tcaactgctt ttttatctaa
      241 attaacggtg cccaatatat tgctctttgt tctcatttga gtaaccaaga taatccctaa
      301 tattaagctt atagaaaaca tcattacttt ttgccagatt ttgttcttca tatttcctcc
      361 gttagtcctc aatatataca ggactgtcac cttttgtaaa atcaattaac cccgtagcta
      421 ccgttaaatt attgtttaat ctttcgttaa ttattttcat tccaaaaaca atctgataat
      481 taatgtcctc gcttaagtct atccttactg cagtgccata ttgtgtttta aagaggaagc
      541 tgttgttcaa ttcgatatta aaggctttta ttatatcgct tccatattca ttgtttaaat
      601 actctattgc attgaatact ttttcattat tttcaacgcc tgcaaattgt atattatccc
      661 ctatattgta tactacatcc gtataacttt caatagttaa taagtctaat ttctcattac
      721 aggttctcaa tactattcca tttttatcga ttattatgta ttcgttgttg aagaaaacct
      781 gatatgtatt caccctttct tttatatcca ggaatatttt attcggaagc tcataaacca
      841 cccttgcatt ttccacgtat acttcatttt caatattctc tttaaccttt gacctgtcaa
      901 cttcaaatat ttttttaccg acttcaatac ctgatttttc cattatatat tcatcactat
      961 atgttaaatt accatcaatg tcaacaccta taaggttgaa atatggagtt ttataatata
     1021 catagcagat acaaaaaaca acaactacag cagccgtaat aaaagctgct gtgctgacca
     1081 taagcttttt atttcgtctt cttttctttt tcctctgtgt ttttttgttg ttgttttcat
     1141 ccattgtttg ttttttctcc cgatgtgtca ttcagtcatc gctatgaata ctcgtttatt
     1201 aattgtcatt ctgagagcta gctcgaagaa tctcatgaga tccttcgctt acgctcagga
     1261 tgacatactt cagaaatcct tcgcttacgc tcaggatgac atatttcaga tatccttcgc
     1321 ttactattaa gatgacaatt tttaaattta tcttacacta ttcaatttcg gtaattatta
     1381 ctcctaagtt ttgtaattta tatataaatt tttcatatcc tctttttata tgatggatat
     1441 tattaatctt ggatgtgcct tctgccacca tagctgcaat tacaagtgca gcacctcctc
     1501 ttaaatcttt ggcttcaact tcggcgctgt aaagtttttc aactccttcc acaactgcta
     1561 ctctcccgat agttgtaata tttgctccca ttttttgcag ctcgggaaca tgttttagcc
     1621 tgctgtcaaa tactgtttct tctataattg aagttccgtc agctacggtc atggatgcca
     1681 taacctgtgc ctgcatatcg gtaggaaaac ccggataggg ttgagttgtt attttttcta
     1741 ttgcttttat ccttttgtta tttgcaataa ctattgttcc gtgtgtttct cttatttcgc
     1801 atcccgcatc ttcatataaa gaagtaatag acttaaaatg agatttttct atgtttctta
     1861 tgtaaatttt gctcttcatc gatgctgctg cactcaaata agttcctgcc tcaatacggt
     1921 cggaaataat tttgtattct acagtatctg tgttttgaac tttttttcct tctatagtaa
     1981 taatacctgt tcctgcacca cttacacaaa aaccacaagt attcaaaaaa ttttgcaagt
     2041 ctgtaatttc aggctccctg gcaggattgt aaatagtagt ctttccgggg tttccgacgg
     2101 caaaaagcat aatattttca gttgccccga cactcggata gtcaagatga atatcacaac
     2161 taagcgcctc atttaccgag ccgaccagca aaccattttt ttcagttatt tcaactccaa
     2221 gctttcttaa tccctttaaa tgcaaatcaa ttggtctaag acctatttca caacctccgg
     2281 gatatgcaac ttccactttc cccgtgcgct gcaaaacagc ccccattaaa attattgagg
     2341 accttatttt ccttactaat ttttcaggta ctatatgagt attcaaacct cttgtatcaa
     2401 ttaacgcaat accattttca aattggacac tgcatcccat attacttaat atttcaagca
     2461 tgtcttttac atcttcaata tcaggtatat tttcaagaat atattctcta ccggtgatta
     2521 cagtgcttgc cagaatagga agagcagcat ttttcgagcc tccgatatcg acaatacctt
     2581 caagactgtt tcttccttca acaataaagc ttcccatttt tatccctccg caacaagctg
     2641 attcataata tatgttatgc acagattttt cacttgttac ttatgtgcat taatgagagc
     2701 catcattgtt gcgtaaattt tattttcaac gtcaaaattt ccgagtctct tggaattaag
     2761 cgccatagtg tttaacctgc tcttattttc aataacttct tcaattgact ttaaaagagt
     2821 tctgccgttt aagtcttttt ccaatataac caatgccgca ccttcctttt ccaaagcacg
     2881 ggcattatat tcctgatgat tttctgcggt atatgttttc ggaatcagaa ttgacggtac
     2941 tcctattgcg gttatttccg caattgttat cgctcctgca cttcctataa caacatcaga
     3001 tgcagaaagg gcacttggag cttcatacat ataatctttt atttcaatat ttttttctaa
     3061 tttaatattc ctgtttaact cttccatgaa tgcatcgtaa tgcatttttc ccgttacatg
     3121 gagtattttt acattttgct tgccattgta tttttttata acatcaatta ttgcttcgtt
     3181 taaacttatt tgtcctccgc tgccgccaaa cgaataaata aaaacatcat ccggttttaa
     3241 attgtacctg gttcttgatt ttctcctgtc tattgttatt atgtcctttc ttaccggatt
     3301 tcccaccatg ataagctttt cttgcgcatt ttggggaaaa tattttttgg cttcttcaaa
     3361 gcttattccc accacatcgg caaatctgct taataatctg ttggttattc cgggaaaaac
     3421 attttgttcc aatatcattg taggaattct ttttaaatga gctgcaaaaa caacgggacc
     3481 gctcacatat cctcccgtac ctacaactat attgggttta aattcattta ttattttaac
     3541 agactcaatt atgccttttg ctgaagcaaa taaggtcttg atattatcaa gggtaaattt
     3601 tcttttgaaa tatttggccg taacagcttt aaattcaaag ccttcctttg gaaccagctc
     3661 gctttcaaga ctgtttttgc ttcccacata caaaacatga tcagaaggat tttcgttttt
     3721 tactttctgt gctatcgcca atgccggatt aatgtgacct ccggtacctc cgcctgttat
     3781 taatactctc ataagaccat tccttttatt aaattaccgc aacatgtgtc acactttgaa
     3841 taaccgttta ttacttgtca ttgctatgaa taatatttat gtcattctga gagctttagc
     3901 tcgaagaatc tcatgagatc cttcgcttcg ctca
//
LOCUS       DAOSOW010000156         3925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_23202_length_3925_cov_3.311886, whole genome shotgun sequence.
ACCESSION   DAOSOW010000156 DAOSOW010000000
VERSION     DAOSOW010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23202_length_3925_cov_3.311886"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PLD67_04995"
     CDS             complement(<1..437)
                     /locus_tag="PLD67_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT21910.1"
                     /translation="MLVYLDNSATTKVRDEVLKEIIDAHENYYGNPSSLHRMGHKVEK
                     KIDEARTLSAKLIGAKPEEIYFTGGGTESNNIAIFGHLSNLKEYNNVITTNIEHPSVY
                     NVFKQLEDRCLVKYLETDENGKINIDELHNMVDENTLLISIMH"
     gene            complement(710..1519)
                     /locus_tag="PLD67_05000"
     CDS             complement(710..1519)
                     /locus_tag="PLD67_05000"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOT21911.1"
                     /translation="MNILSDKNVMNNQIITKEETELLINKTKIYEVVRVIDGKPIFLK
                     EHFQRMSESINLSGIEGNINYEEFKNSVELLIKENSFKNLNIRVSYFYDKKPLTLFYF
                     IESYYPSKDEFAEGVHTVTVKMERANPNVKSFQKEYKDKVTRIIKENNAFEAILINHD
                     NTVSEGSKSNIFFVEGNKLITSPDSSVLLGVTRSKVISVCEENSIEVHKRIIRLDEIE
                     SFDGVFLTGTSNDVLPIKTVDNIVFNSSENEVVKKVSRLYLGEVKKEIEKG"
     gene            complement(1533..3833)
                     /locus_tag="PLD67_05005"
     CDS             complement(1533..3833)
                     /locus_tag="PLD67_05005"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HOT21912.1"
                     /translation="MDNIKLSVRELVEFIYKSGDINVRNLGADRAIEGIKAHKILQNQ
                     MGENYYKEYYLKGEFVLNDIIFLIEGRADGILIEDDETIIDEIKSTYTDLSLINGEYS
                     TAHMAQAKCYGYMYGLSNNLENISVQLRYYNLDTKEVKTIKQTFSTEELKIFFYSLLN
                     TYIDWAETIINLRKERNRTIKELNFPFEKYRKGQRDFSIAVYLSIRSGKKLFAQAPTG
                     VGKTVSVLFPAIKSLYEKNAKIFYLTAKSSTKTIAFNTVKMMHKQGLRLRTTVITAKD
                     KICFMDETKCEPEYCPYAKGYYDKLNIPLRETIKNNCMYDREFIEELGKKYELCPFEL
                     SLDLSYMSDIVICDYNYYFDPRVALKREDLFNKDKDILLIDEAHNLEDRVRNMYSPEL
                     IKEEFYEVYKIIKSEKSLSKELFNINKKFINIKKSIDEAQILKETPEELINALRKFIA
                     KADKYFNERKNEKIPEELTDIYFKSIFFIKISEIADDNFCYYADSANNKTMVKLFLID
                     TSQILKEIERNARSSIFFSATLTPLKYFRYILGGEEDDYILKLSSPYDKENLNLMITS
                     DISMKYTKRDSNIEKTCEYINTLITEKKGNYMIFFPSYNFMNKVYSVYESLYDTMNII
                     VQSQGLNEEEQMDILSRFENERNVVLFTVVGGTFSEGIDLPLDKLIGAVIIGTGIPQI
                     SFERNIIKAFFDEKMNTGYDYAYKYPGFNKVLQSAGRVIRTEDDRGTVLLIDSRFCQY
                     TYLKLFPDHWKHFKKIKTSEDIKNLL"
BASE COUNT         1147 a          733 c          529 g         1516 t
ORIGIN      
        1 tcatgcataa ttgatatgag gagggtattt tcatccacca tattatgaag ctcatcaata
       61 ttaatttttc cgttttcatc cgtttctaaa tacttaacca aacatctgtc ttctaactgt
      121 ttaaaaacat tgtatacgga aggatgctca atattagtgg tgataacatt attgtactcc
      181 tttaggtttg ataaatgtcc aaaaattgca atattattgc tttccgttcc tccgccggta
      241 aaataaattt cttcaggttt tgcacctatg agttttgccg aaagtgttct tgcttcatca
      301 atcttctttt caaccttatg tcccatcctg tgcagggaag aaggattccc gtaataattt
      361 tcatgagcat ctattatttc tttcaatact tcatctctta cttttgtcgt tgcactgtta
      421 tccaaatata ctaacatatt tacctctttg tttattttta taagtttctt ttaagattct
      481 ttgacaagcc cttcatctaa cggtgccagg caccatgctg aggtgccagg cacctggaga
      541 aggcacctag ggaactcgaa atttgtaaaa aaagcttgca taataatttt atgtttgcta
      601 taattgttat tggttatccc ccccgataac caaatatatt cccaataccc cttttatgca
      661 tattaatgct tcacaacaat ggccaccagg ccatttttgt gtttatgacc taacccttct
      721 caatctcttt tttaacctct cctaaatata atctggacac cttcttcaca acttcatttt
      781 cggaagaatt aaagacaata ttatcaacgg tttttatcgg aagtacatca tttgatgtac
      841 ctgttaagaa tacgccgtcg aaactttcta tctcgtccaa tctaattatc cttttatgaa
      901 cctctatcga attttcttca caaacgctta taaccttact tcttgtgacg cccaaaagaa
      961 cagaagaatc cggcgatgtt atcagcttat tgccttctac gaaaaaaata tttgatttgc
     1021 ttccctcgct gaccgtgttg tcatgattta ttaatatggc ttcaaaagca ttattttctt
     1081 taattattct tgtaacttta tccttatatt ctttttggaa tgacttaaca ttggggtttg
     1141 ctctctccat tttcaccgta acggtatgta ctccttcagc aaattcgtct ttggaaggat
     1201 agtaactttc aataaagtag aacaaagtaa gaggcttttt atcataaaag taagaaactc
     1261 taatgtttaa atttttgaag gaattctctt ttataagcaa ttctacggaa tttttgaact
     1321 cttcataatt tatgtttcct tctattccgc ttaaatttat gctttcgctc atacgttgga
     1381 aatgttcctt taaaaaaata ggctttccat caataaccct tactacctca taaattttag
     1441 tcttatttat aagaagctct gtttcttcct ttgtaataat ttgattgttc attacatttt
     1501 tatcggataa tatattcata tgtcctccta tactacaaca aattttttat atcttctgaa
     1561 gtcttaattt tcttaaaatg tttccagtgg tcgggaaata atttcaaata tgtatattga
     1621 caaaatcttg agtcaatcaa caggactgtt cccctatcat cttcagttcg tattactctt
     1681 cctgctgatt gaaggacttt gttgaagccg ggatatttat atgcataatc ataacccgtg
     1741 ttcatttttt catcaaaaaa tgctttaatt atatttcttt caaaagaaat ttgcggtatc
     1801 ccggttccta ttataactgc gccaattaat ttatcaaggg gtaaatcaat tccttcagaa
     1861 aaagtacccc ctacaacagt gaatagaacc acattgcgct cattttcgaa cctgcttaag
     1921 atatccatct gttcttcttc attgagcccc tggctttgaa caattatgtt catggtatca
     1981 tacaatgatt catataccga ataaacctta ttcatgaaat tataagaggg gaaaaatatc
     2041 atataatttc cctttttttc tgttatcaaa gtattgatat attcacatgt cttttcaata
     2101 tttgaatccc tctttgtgta tttcattgat atatctgaag ttatcattaa gtttaaattc
     2161 tctttatcat aaggagagct taactttaat atataatcat cttcctctcc ccctaatata
     2221 tatctgaaat atttcaaggg agttaaagtt gccgagaaga aaattgatga ccgggcattc
     2281 ctttcaattt ccttcaatat ttgggaagta tcaatcaaga ataatttcac cattgtttta
     2341 ttgtttgcag aatcggcata atagcaaaaa ttatcatccg caatttccga tatttttata
     2401 aaaaaaatac tcttaaaata tatgtcggta agttcctccg gaattttttc attttttctt
     2461 tcgttgaaat atttatcagc cttagcgata aattttctta aagcatttat taattcttcc
     2521 ggagtttctt ttaatatttg tgcttcatct atactctttt taatatttat aaatttttta
     2581 ttaatattaa ataattcctt gcttaagctt ttttcagatt taataatttt atatacttca
     2641 taaaattctt ccttgattaa ctccggagaa tacatatttc gtactcggtc ttcaagatta
     2701 tgcgcctcat ctatgagcag tatatccttg tccttattaa atagatcctc tcttttcagt
     2761 gctaccctcg ggtcaaaata atagttgtaa tcacatatta ctatatctga catataggac
     2821 agatcaaggc tcagttcaaa agggcacagt tcatactttt ttcctaattc ttcaataaac
     2881 tcacggtcat acatgcaatt attctttatt gtttctctta atggaatatt aagcttgtca
     2941 taataaccct ttgcataagg gcagtattcc ggctcgcatt ttgtttcatc catgaagcaa
     3001 attttgtcct tggcggttat aacggttgtt ctcagcctaa gaccctgttt atgcatcatt
     3061 ttaacagtgt tgaaagctat agtctttgtt gaagacttgg ccgtaagata gaatatttta
     3121 gcattttttt catacaggga ttttattgca ggaaataaaa cggatactgt tttccccact
     3181 cccgtaggtg cctgtgcaaa aagttttttt cctgacctta ttgataaata tacagctatc
     3241 gaaaaatctc tttgtccttt tctatatttt tcaaaaggaa aatttaattc ctttattgtc
     3301 ctgtttcttt cttttcgaag atttataatt gtttctgccc aatcaatata tgtattaaga
     3361 agagaataaa aaaatatttt taattcttct gttgaaaatg tctgctttat tgttttcact
     3421 tctttggtgt caagattata gtacctaagc tgcacactga tattttccaa attgtttgag
     3481 agtccgtaca tataaccgta gcatttagcc tgagccatat gggcggtgct gtattcacca
     3541 ttaatcaaac ttaaatccgt ataagttgat tttatttcat caattattgt ttcatcgtct
     3601 tcaattaaaa ttccgtctgc tctgccttca atcaaaaata taatatcgtt aaggacaaat
     3661 tcgcctttca gatagtattc tttataataa ttttctccca tttgattctg gagaatttta
     3721 tgggctttaa ttccttcaat tgctctatct gcacctaaat ttcttacatt aatatcgccg
     3781 cttttataaa tgaattcgac caattctctg acagaaagtt ttatattgtc cattattact
     3841 ccttgaacag ggacatcggt gtgtccccat accataaaaa ataaacactc gtagtgttta
     3901 ttatagcaaa ttttgtatgt tattt
//
LOCUS       DAOSOW010000157         3921 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_23227_length_3921_cov_3.361097, whole genome shotgun sequence.
ACCESSION   DAOSOW010000157 DAOSOW010000000
VERSION     DAOSOW010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23227_length_3921_cov_3.361097"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="PLD67_05010"
     CDS             <1..121
                     /locus_tag="PLD67_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT21913.1"
                     /translation="NGDWNFGSAISIVMMLIILLSMSIMNKFDVDKEGGARLW"
     gene            115..915
                     /locus_tag="PLD67_05015"
     CDS             115..915
                     /locus_tag="PLD67_05015"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT21914.1"
                     /translation="MVKKYLSKIYMGIIFIFLYAPIIVLGVFSFNESKSRGNWTGFSL
                     KWYIELFHDHDIRMAFYYTITIAIITAIVSTILGTIAAIGINAMKGRQQALILNVNYL
                     PVVNPDIVTGISLMILYISFNMDFGFMTMLLSHIVFSTPYVILSILPKLKQLDVHMTE
                     AAMDLGATPMYALRKVILPEIKPGIITGFLMAFTLSIDDFIISYFTKGEGVTNLSIVI
                     YSMARRGVRPTINALSTIMLIVVLLLMILINKRTNGHDKPKKIYRSEV"
     gene            917..1957
                     /locus_tag="PLD67_05020"
     CDS             917..1957
                     /locus_tag="PLD67_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOT21915.1"
                     /translation="MKRKLILTFLVLSLIILAATGCSSEEKQTLSILNYDIYIDKSLL
                     TEFEKANDVTIKYDTYSTPEEMYIKVKSGASKYDLIISSEYMIERMINEKLVNKLNFD
                     NIPNYKYIGEDFKNHVYDPDNEYSVPYFWGTLGILYNKKTVDASSDSWAMLWDENHAQ
                     KIIMMDSQRDSFAAALKLLGYSINTVDEGELDEAKELLIKQRPLVMAYITDGSPAIMV
                     TEEADMALVWSGEAVAAMSENENLDFVIPKEGSNIWIDAMVIPSTTQNQELAEKFINF
                     LCSTEATLRNIDEVWYSTVHTEAIKQVDEELLNNPAFNIPKEDIAKMEMLRDPKEFIE
                     LYTERWTELMAQ"
     gene            complement(2023..2859)
                     /gene="ytxC"
                     /locus_tag="PLD67_05025"
     CDS             complement(2023..2859)
                     /gene="ytxC"
                     /locus_tag="PLD67_05025"
                     /inference="COORDINATES: protein motif:HMM:NF020391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YtxC"
                     /protein_id="HOT21916.1"
                     /translation="MELLSLELNENENHEIYNNIRIITNIEESDINIELSSTDEGNIK
                     IRYFTDNKINKREYLEKITAKITKLLVEYTKLVGIKILKENYFYFDDEEVSTIISDIE
                     EEIDNDIKIHIIIKNKFKEVIEKSNYINLNGFVNFRLKFLKLYAAQVVEKCIDSYLMK
                     KEYMDFISIIKLISDSEENQYDIVNVMFNNNKIQVYDKNMQKLNYFSNVELSTELNTE
                     STYDETVINILMSVSPKKIILHDLKIDKKNKEAYNTADIIKRIFEGKVETCSGCKYCE
                     FL"
     gene            complement(2887..3621)
                     /locus_tag="PLD67_05030"
     CDS             complement(2887..3621)
                     /locus_tag="PLD67_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21917.1"
                     /translation="MNPYIPTQKPRYVIIDYRASEEILNYLKKLNIEPIKSIKCNELH
                     EPVAGHPDMVIFPIDFKTFVAAPNVYDYYRNVLGSLGIKVIKGGKTLSRNYPEDIAYN
                     VARIGRYALHNTKYTDQVLKYYLEEAGIQFIHVNQGYTKCSIAPVSDNKALTSDFLIH
                     EKLISYNIDCMYIDPKVVYLKGYNNGFIGGCTGLINEKIFLSTGKIFDENISVSLREF
                     IQSSGYIYDEASKQQITDLGTLIPII"
BASE COUNT         1269 a          634 c          621 g         1397 t
ORIGIN      
        1 aaacggagac tggaatttcg gctcagccat atccattgta atgatgctta taattctttt
       61 atccatgtca attatgaata aatttgatgt tgataaggaa ggaggagcaa ggttatggta
      121 aaaaaatatt tatcaaaaat atatatgggc attatattta tattcctcta tgctccgatt
      181 atagttcttg gtgtattttc ctttaatgaa tcaaaatcga gaggaaactg gacaggattt
      241 tcactgaagt ggtacattga gcttttccat gaccacgata tacgaatggc tttttattac
      301 acaataacga tagcaatcat tacggcaata gtatctacaa ttttgggtac aatagcagca
      361 ataggaataa atgcaatgaa gggcagacaa caagctttaa tactcaatgt taattacctt
      421 ccagttgtaa atcccgatat tgttacgggt atatccctga tgattttata tatttcattt
      481 aatatggatt ttggatttat gacaatgcta ttgtctcata tagtgtttag cactccctat
      541 gtaatattgt caatacttcc aaagttaaag cagctcgatg tacacatgac cgaagctgca
      601 atggatttag gggcaactcc catgtatgct ctaagaaagg taatacttcc tgaaataaag
      661 ccgggaatta ttacgggatt tttgatggca tttacattgt ccattgatga tttcatcata
      721 agttacttta ccaagggcga aggtgttaca aacctttcaa ttgtgattta ttcaatggca
      781 agaagaggtg tccgccctac aatcaacgct ctttcaacaa taatgctgat agtagtatta
      841 ttgttaatga tattgataaa caaaagaact aacgggcatg ataaacccaa aaaaatatat
      901 cgaagtgagg tatagaatga aaagaaaatt aattttgaca tttttagttt taagcttgat
      961 aattcttgct gcaaccggat gcagctctga agaaaaacaa acattgagta ttttaaacta
     1021 tgatatttac attgacaaaa gtttattgac cgaatttgaa aaagccaacg atgttacaat
     1081 taaatatgac acgtattcaa cgccggaaga aatgtatata aaggtgaaat ccggggcttc
     1141 aaaatatgat ttgattattt caagcgaata tatgattgag cgcatgatta atgaaaaatt
     1201 ggttaataaa ttaaatttcg ataacattcc gaattataaa tatatcggcg aagattttaa
     1261 aaaccatgta tatgaccctg ataacgaata ttcggttccg tatttctggg ggaccttagg
     1321 catattgtac aataaaaaaa ctgtagatgc atcttctgac agctgggcta tgctttggga
     1381 tgaaaatcat gctcaaaaaa ttataatgat ggattcgcaa agagattcat ttgcagctgc
     1441 gctaaaatta ttgggatatt caattaacac ggttgatgaa ggagagctcg atgaagcaaa
     1501 ggaactcctt attaagcaaa gacctcttgt tatggcttat ataacggacg gttcacctgc
     1561 aattatggta accgaagaag ctgatatggc gcttgtttgg tcaggtgaag cggttgccgc
     1621 aatgtccgaa aatgagaatt tggattttgt aattccaaag gaaggcagca atatttggat
     1681 tgatgctatg gttattcctt cgactaccca aaatcaggaa ttagccgaaa aatttattaa
     1741 cttcttatgc tccaccgaag caacgttaag aaacatagac gaagtatggt attctacagt
     1801 acataccgag gctattaaac aagtggatga agaactctta aacaaccctg cttttaacat
     1861 tccaaaggaa gatatcgcaa agatggaaat gttgagagac ccgaaagaat ttatagaatt
     1921 atacactgaa agatggactg aattaatggc acagtaatca tctttaaatt aaaaccggtg
     1981 ccgggcaccc aatagggtgc ccggcaccgc ttcatatata ttttataaaa actcacaata
     2041 cttacatccg ctgcaagttt cgactttacc ttcaaatatt ctttttatta tatcagcggt
     2101 attatatgct tctttatttt tcttatctat ctttaaatca tgcaatatta ttttttttgg
     2161 tgacacactc attaaaatat ttattacagt ttcgtcgtat gttgattcgg tattcaattc
     2221 agttgaaagc tctacatttg aaaaataatt gagcttttgc atattcttgt catatacctg
     2281 gattttattg ttattaaaca ttacatttac aatatcatat tgattttctt ccgaatctga
     2341 tatcagtttt ataatgctta taaaatccat gtattccttt ttcatcaaat atgagtcaat
     2401 gcacttttct actacctgtg ctgcataaag ctttagaaat tttagtctga agtttacaaa
     2461 tccgtttaaa tttatataat tggacttttc tattacttct ttaaatttat ttttaattat
     2521 tatgtgtatt ttaatatcat tgtctatttc ctcttcgata tcggatatta ttgtgcttac
     2581 ttcttcatcg tcaaagtaaa aataattttc ctttaaaatt tttataccta ctaacttagt
     2641 gtattcaacc aaaagcttgg ttatttttgc tgttattttt tcaaggtact ctctcttgtt
     2701 tattttatta tccgtaaaat atcttatttt tatatttcct tcatcggtag acgataattc
     2761 tatatttata tcactttcct ctatgttagt tataatcctt atattattat aaatttcatg
     2821 attttcattt tcatttaact ctaatgatag tagctccact tagtcactcc tctcggtcaa
     2881 gggaggttat attatcggta tcagcgttcc taagtctgta atttgctgtt ttgaagcttc
     2941 atcataaata tatcctgacg actggatgaa ttctcttaat gaaacagaaa tattttcatc
     3001 aaaaattttt ccggttgaca aaaatatttt ttcattaatc aatcctgtac agcctcctat
     3061 aaatccgttg ttgtatcctt ttaaatatac aacttttggg tctatataca tgcagtctat
     3121 attgtatgat attagttttt catgtatcaa gaaatccgat gtcaaagctt tattatcact
     3181 gacaggagca attgagcact ttgtatagcc ttgatttaca tgaatgaatt gaatgcccgc
     3241 ttcttccaaa taatatttta aaacctggtc tgtatacttt gtattatgca aagcatatct
     3301 ccctattctt gctacattat atgctatatc ctcaggatag tttctgctaa gagtttttcc
     3361 acctttaata acttttattc ctaggcttcc caaaacattt cggtaataat cataaacgtt
     3421 tggagctgca acaaaagttt taaaatctat gggaaaaatt accatatccg gatgaccggc
     3481 cacaggctca tgcagctcat tgcatttaat ggattttata ggctctatat ttaatttttt
     3541 tagataattc agtatttctt cacttgctct gtaatcaata attacatagc gaggcttttg
     3601 agtcggaatg tatggattca tattatctcc cgttgaatga gtctcattca cagattatat
     3661 cattaaggta tatattattc tacaaaaaat catgaggttc ttcaagctag ctctcagaac
     3721 gacaaaaatg ctatttatag taaaataaaa tgatataatt ataaaattat atcattttta
     3781 tattaatcca aataaattta tcaggcaatt tctacaagtg agttgattta tcctcctgcc
     3841 aagagatgta taatttcaac attatcaccg tcattaataa tagttgttgg gtaatcttcc
     3901 ttttcgatat gttttccgtt t
//
LOCUS       DAOSOW010000158         3906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_23356_length_3906_cov_3.534147, whole genome shotgun sequence.
ACCESSION   DAOSOW010000158 DAOSOW010000000
VERSION     DAOSOW010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3906
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23356_length_3906_cov_3.534147"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            229..1197
                     /locus_tag="PLD67_05035"
     CDS             229..1197
                     /locus_tag="PLD67_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOT21918.1"
                     /translation="MINISDKIKKKLDMLPMEPGIYKMIDAKGNVIYIGKSKMLNKRV
                     KTYFANNPNWDKINRMVRLIDDIDFIVTDTHLEARILECSLIKELKPIFNSQMKHDRG
                     YVYLKIENYNKNRALSVVESREESCFGPFRKKFHLNETINSLKNIYPIIKKGESYSFD
                     FHLFPLTLNEIEFNKNRYSLYEILTDNSKAGIFVKELENKMKREALLCDFEKASFYKG
                     ILINVRYLINSINKYNELLKSNVIVSIPFDEGYKLFYISKGIILKKEVFKAPNKKDIE
                     IFSKAALTSPDDSFINMDEKTLMDYKDIICSEIISLSDTVVKYMDI"
     gene            complement(1207..1908)
                     /locus_tag="PLD67_05040"
     CDS             complement(1207..1908)
                     /locus_tag="PLD67_05040"
                     /inference="COORDINATES: protein motif:HMM:NF016154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HOT21919.1"
                     /translation="MSWTEITYRVIFSFIVLLIMARLMGKEHMTQLTFFDYVTGITVG
                     SLAAAVVTDTSLEMIKGIYALILWSILNIIIEFVILKFDKSRLLITGKPSILIKRGEI
                     DKKALAKCRFNADELNMMLRNKDIFDITEVDYAILETSGKLTVLKKQKFDPITKKDMP
                     IPVKQNRNLPVQIINNGKIIESVMTEFNLDIDWVYSEAKRLGYKNIKDIFYAGINCDG
                     QFFIVPLFNEKKYKQ"
     regulatory      2085..2186
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2237..3418
                     /gene="cytX"
                     /locus_tag="PLD67_05045"
     CDS             2237..3418
                     /gene="cytX"
                     /locus_tag="PLD67_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009985865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HOT21920.1"
                     /translation="MGSYKTSTLSNGLLWFGAAVSIAEIFTGTLFVPLGFSKGFIAIV
                     IGHIIGCILLYYAGIIGAKSGKSAMESVGLSFGNKGRNYFAVMNVLQLVGWTAVMIIQ
                     GARAMGVMLNTNLGMQSDLLWGIIIGAAILVWVVIGPKNLEKVNIFAVGSLFILTIFL
                     GATVFNRGSNANISGEMGFGAAIELSAAMPISWLPLISDYTRDAKHPITATRVSVITY
                     FAGSVFMYTIGLGSAIFTGESDIAVIMYKAGYGILGVLIILFSTVTTTYLDVYSAGVS
                     LKTIHAGLPEKTMAIAVGLIGTAIAIFTPVEQYQNFLYLIGSVFAPMISILIADYFIL
                     KKDNIKKSVDVTNFIIWLIGFTIYRLLLNMNLAIGSTIPVMIITVIICVIADKIRKLK
                     V"
     gene            3544..>3906
                     /gene="thiW"
                     /locus_tag="PLD67_05050"
     CDS             3544..>3906
                     /gene="thiW"
                     /locus_tag="PLD67_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HOT21921.1"
                     /translation="MIKNKEINLKKLVFASLLVCMGYVLNTFIWIPGMAPFQHFINVT
                     AAVFLGPAWAFLCALTIGLLRMIINGRTILALIGAIVGAFLSGILYKRFQKLYWAVIG
                     EVIGTGIISAILSYPFMRM"
BASE COUNT         1347 a          543 c          704 g         1312 t
ORIGIN      
        1 gggaaacggg atttaacata catttggcta aacctataag ctttaacata ttgggaaaag
       61 tacttaaagg tgtaaaatta aaatgcatgc agtgaacaga taatattata ttttaagagc
      121 agagtgccgt ttgaaacaag gagaagaaaa gtctttaact aatataatga aactgtatgt
      181 aaagctttaa tagtaaagaa cactaaccag gaagaaacag gtgatttatt gataaatatt
      241 tctgacaaaa taaagaaaaa attagatatg cttcctatgg agccgggaat atacaaaatg
      301 attgatgcaa agggaaatgt tatttatata ggaaaaagca aaatgctgaa taaaagagta
      361 aaaacatact ttgcaaacaa tcctaattgg gacaagatta acagaatggt tcgtttaata
      421 gatgatattg attttattgt aactgatact catctagagg ctcgtatttt ggaatgcagt
      481 ttaataaaag aattaaaacc tatttttaat tcacaaatga agcacgacag gggttatgtt
      541 tatttaaaga ttgagaatta taataaaaac agagccttat cagttgttga atccagagaa
      601 gaaagttgtt ttggaccttt tagaaaaaaa tttcatttaa atgaaactat caacagttta
      661 aaaaatatat accccataat taaaaagggt gaatcatatt cctttgattt tcacctgttt
      721 cctctaacac taaatgagat tgaatttaac aaaaacagat attctttata tgaaatatta
      781 actgataatt caaaggcagg tatatttgtt aaagaattag aaaataaaat gaaaagagaa
      841 gctttattat gtgattttga aaaggcttct ttctacaaag gcatcttaat taatgtaaga
      901 tatcttatta attcaatcaa taaatacaat gaacttctta aaagtaacgt aattgtatca
      961 attccgtttg atgaaggcta caagttgttt tatatttcaa agggaataat acttaaaaaa
     1021 gaagttttta aagctcctaa taaaaaagat attgaaatat tttctaaagc tgccttaact
     1081 tctccggatg atagttttat caatatggat gagaagaccc ttatggatta caaggatatt
     1141 atttgctcag aaataatttc tttgtccgat actgtggtaa aatatatgga tatctaggcc
     1201 attgatttat tgtttatact ttttttcatt aaagagcgga acaatgaaaa attgcccgtc
     1261 gcaattgata cctgcataaa aaatatcttt tatattttta tatcccagtc tttttgcttc
     1321 gctatatacc cagtcaatat caagattaaa ttctgtcata acactttcga taattttacc
     1381 gttattaatt atttgcacag gcaagtttct attttgctta accggtatgg gcatatcttt
     1441 tttcgttata gggtcgaatt tttgcttttt taatactgtt aatttgccgc ttgtctcaag
     1501 aatagcataa tcaacttcgg taatatcaaa aatatcctta ttccggagca tcatatttaa
     1561 ttcatctgcg ttgaatctgc atttggccaa tgcttttttg tcaatttctc ctcgtttaat
     1621 taagattgaa ggcttacctg ttattaacaa tctggactta tcaaatttta atataacaaa
     1681 ttcaattatt atgtttagta ttgaccataa tattaatgca taaatcccct taatcatttc
     1741 aagacttgta tctgttacta ctgcggcagc taatgaacct acggtaattc ccgttacata
     1801 atcaaaaaag gtaagctggg tcatatgctc tttacccata agtctcgcca ttattaataa
     1861 aactataaaa gaaaaaatca ctctataagt aatttcagtc caagacattt tactctccaa
     1921 ccataatatt attgatagtc tttcaatata atagtttatt attcaatgat tcaataagcc
     1981 cccttaatgt tacatgaatg aaaattaccc taacagcaaa aacattggat tatgaaaaat
     2041 agattgcatt tactgtaggc ttgttgtata atcaaattaa ttaaattaat aatatcggga
     2101 gcataatagg ctgagaggaa acttaaagtt tcgaccgtac ctgatttgga taatgccaac
     2161 gtagggaact gtttatgaga gtgaatggga tacaccgtgt tggtatatcc catttttagt
     2221 aggaaggaca taagttatgg gtagttacaa aacatcaacg ttaagcaacg ggttactctg
     2281 gtttggagca gccgtatcta ttgcggaaat atttacggga acattatttg taccgcttgg
     2341 tttttcaaaa ggatttattg ctattgtgat tggacacata ataggctgca tacttttata
     2401 ttatgccgga atcataggtg ctaaaagcgg taaatcagct atggaatctg tcggattatc
     2461 atttggaaac aaaggaagaa attattttgc cgtgatgaat gtattacagc ttgtcggatg
     2521 gactgccgta atgataattc aaggcgcaag agcaatgggg gttatgttaa atacaaatct
     2581 cggtatgcaa tctgatttgt tgtggggcat tattataggg gctgcaatat tagtatgggt
     2641 tgttattgga cctaaaaact tggagaaggt aaatattttt gcagtgggaa gcttgtttat
     2701 tctgacaatt tttctaggag ctactgtttt taacaggggt tctaatgcaa acatttccgg
     2761 tgaaatgggt tttggagcag cgatagaact gtctgcagct atgcctattt cgtggcttcc
     2821 gcttatttct gattacacaa gggatgcaaa gcatccaata actgcaacac gggtaagtgt
     2881 gattacatac tttgcaggca gtgttttcat gtatacaatt ggtttgggat ctgctatctt
     2941 taccggcgaa agtgatatag cggttataat gtataaggca ggatatggca ttttaggcgt
     3001 attaataata ttgttttcaa cggtaacaac gacgtatttg gatgtttatt cggcaggagt
     3061 aagcttgaaa acaattcatg ccggattgcc tgaaaaaacc atggctattg cagtaggttt
     3121 aattggcact gccattgcaa tttttacacc tgtcgagcaa tatcagaatt ttttatattt
     3181 gataggctca gtgtttgctc caatgatttc gattctcata gcagattatt ttatacttaa
     3241 aaaagataat ataaaaaaat ctgtggatgt gacaaatttc attatttggc tgataggatt
     3301 tacgatttac agactgcttt taaacatgaa tttggctatt ggaagcacaa tacccgtaat
     3361 gattataacc gtaatcatat gtgtaatagc agataaaata agaaagctta aagtataatt
     3421 taataataac aataatatta ttgacagaat gttgaaagca taaaaagggg tgcaccaccg
     3481 cgggtacaat cttcttttta tgctttttta ataattaatt aaaacaaatt taaggaggaa
     3541 gaaatgataa aaaacaaaga aattaattta aaaaaacttg tttttgcttc attgttagtg
     3601 tgtatgggat atgttctcaa tacttttata tggataccgg ggatggctcc gtttcagcac
     3661 ttcataaatg taacggcagc tgtattttta ggacctgcat gggcattttt atgtgcattg
     3721 acaataggat tattaagaat gataataaac ggccgtacaa tattagcgct tatcggagca
     3781 attgtgggag catttttatc aggaatacta tataaaaggt ttcaaaaatt gtactgggcg
     3841 gttataggag aggtaatcgg taccggtata ataagtgcaa ttctttcata tccctttatg
     3901 agaatg
//
LOCUS       DAOSOW010000159         3898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_23422_length_3898_cov_3.483997, whole genome shotgun sequence.
ACCESSION   DAOSOW010000159 DAOSOW010000000
VERSION     DAOSOW010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23422_length_3898_cov_3.483997"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLD67_05055"
     CDS             complement(<1..487)
                     /locus_tag="PLD67_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOT21922.1"
                     /translation="MNNIDPRTKLIQVLILSTLALIYNEITLLSIILIAAIMIALINN
                     VNFISVIIRLNKLLKIIFLIALIQSLLTNMGNPIFKMGNFTLFTDYGILKSFEFILRM
                     GIIIVSAAIITTSSSREIIQGLIQWNCPYEIAFMVSVAVRFLPIFKEEMTDIITAIQL
                     RG"
     gene            complement(480..1133)
                     /locus_tag="PLD67_05060"
     CDS             complement(480..1133)
                     /locus_tag="PLD67_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21923.1"
                     /translation="MNNKIEINNISYEYPRGRKVFINFSLELNKGETTVITGKNGCGK
                     TTLAKLIMGILKPSFGEIKLFGQSTKDMSLGAIGEIIGYVFQSPERQLFGTTVLEELT
                     FPLLFKGYNREETMDRAEKMLEIFDLKKVRNSYPYFLSYGEKRCLAIASVLMVGPEYL
                     ILDEPTASLDKERIEALSQALENLKKRNIGMLIISHNKDFIERHKDRIINLEGGSYE"
     gene            complement(1133..1789)
                     /locus_tag="PLD67_05065"
     CDS             complement(1133..1789)
                     /locus_tag="PLD67_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21924.1"
                     /translation="MMNAVEVKNLCFKYDNRKEYIFKDVNFNVRTGETIAITGRSGSG
                     KTTLCHCICGLVPRLYKGELTGEVLIFGENIKNLSIAEVVTKIGIIFQNPSTQLFSPT
                     IEDELAFGPENLCVEREEINSRIDEILRIIKMEKYRYDNPNNLSGGQQQLIAIASVLM
                     LKPSILICDEIMSWIDDEGRKIIKDLLLKLKKEGKTIILVDHDNDNLNIADRIIHIGE
                     "
     gene            complement(1786..1911)
                     /locus_tag="PLD67_05070"
     CDS             complement(1786..1911)
                     /locus_tag="PLD67_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21925.1"
                     /translation="MEDGRYLGSRDSGGRGPYESIVVSDFFSNRRCKWITEIEVK"
     gene            complement(1989..2297)
                     /locus_tag="PLD67_05075"
     CDS             complement(1989..2297)
                     /locus_tag="PLD67_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21926.1"
                     /translation="MKKRALIIVLLVIILTITLIISLNLNKASIEKQKQILSNAQIVL
                     KYNDKEKVLTKEEIILYGEDFEAVLDTSTTEPSTHIYRGVQLKDLLNNHNIDLQDKLL
                     "
     gene            complement(2294..3334)
                     /locus_tag="PLD67_05080"
     CDS             complement(2294..3334)
                     /locus_tag="PLD67_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOT21927.1"
                     /translation="MKLKKILSAINYTEHSLGIKLFLLIFLVILVGCSADVNYKESTG
                     INIFNENKNFFVDFKSEKEISINEITNDKFSEAAVILNTGEIIQVPAEFYIKNNTVTA
                     DGETVENTVGIYLTDNYQSITNAYYDMKEYLDKGEKVMTVLLDGFSYNQFKRANEENT
                     LTFLSKYFQYPALSVYTPVTNAGFAAIITGQAPDVNGVHDRSNRRMKVDSIFKYALDN
                     NKSCILLEGDIKILNTEIEPELHTDINKNSETDDEIYESALISVNEDYDLIFIHFHGI
                     DDRGHSFGPYSREVMDYIKTIDKYIKDLSEEWEGAIILVPDHGMHETAEGGGHGICRQ
                     SDMVVPYFIKEP"
     gene            complement(3337..3888)
                     /locus_tag="PLD67_05085"
     CDS             complement(3337..3888)
                     /locus_tag="PLD67_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOT21928.1"
                     /translation="MTNKFLEKFSVYNLIIIAVVSALGIATKPIVVPLVHIITGPLFI
                     PGGAIAGGFYMFWIVLGAGIVKKSGTGTLIGIVQGILVIATGTMGTHGIMSIVSYTLP
                     GIVTDIIFMFSINKKYNILHYVFGCLAANVTGTLISNILFFRLPFVTVILILSAAALS
                     GATGGLIAYAINRSLEKMKILED"
BASE COUNT         1174 a          751 c          552 g         1421 t
ORIGIN      
        1 ttcctcttag ttgtattgca gttattatat ctgtcatttc ttctttaaaa ataggtaaaa
       61 accttactgc gacagaaacc atgaatgcta tttcataagg acaattccac tgaataagtc
      121 cctgtattat ttccctgctt gatgatgttg ttattattgc agcagaaact atgattattc
      181 ccatgcgcaa aataaattca aatgatttta aaattccgta atcggtaaaa agcgtaaaat
      241 tacccatttt aaaaatagga tttcccatat ttgtcaataa actctgaatt aacgcaatca
      301 agaatattat ttttaataac ttattaagtc ttataattac tgatataaaa tttacattgt
      361 taataagtgc aatcataatt gcagcaataa gtatgattga taaaagtgtt atttcattat
      421 aaattaatgc aagggttgat agaattaaaa cttgtattaa ttttgtccgg gggtctatat
      481 tattcataac ttcccccttc aagattaata attctgtctt tatgcctttc gataaaatcc
      541 ttgttatggc ttattataag catcccgata ttcctttttt ttagattttc aagagcttgc
      601 gataatgcct ctattctttc tttatcaaga gaagccgtag gctcatccaa aataagatat
      661 tcaggaccaa ccatcaatac ggaagcaatt gctaaacatc ttttttctcc gtaactcaaa
      721 aaataggggt atgaatttct aacttttttc aaatcaaaaa tttccagcat tttttcagcc
      781 ctatccatag tttcttccct gttgtatcct ttaaacaaca aaggaaatgt caattcttcc
      841 aatacggtag tgccaaaaag ctgcctttcg ggactctgga aaacatagcc tataatttca
      901 cctatggctc caagggacat atcttttgtg ctttgaccga atagttttat ttccccgaat
      961 gaaggtttta atattcccat aattaactta gccaaggttg tttttccaca accgtttttc
     1021 cccgttataa cagttgtttc ccctttattt aattcgagag agaaatttat aaataccttt
     1081 cttcctctcg gatattcata tgaaatattg ttgatttcaa ttttattgtt cattattctc
     1141 ctatatgtat aatcctatcg gctatattta gattatcatt gtcatggtca accagaatta
     1201 ttgtttttcc ttcttttttt aacttcagta aaagatcttt gattattttt cttccctcat
     1261 cgtcaatcca tgacataatt tcatcgcata tgagaattga aggtttaagc attagtacag
     1321 atgcaattgc aattagctgc tgctgacctc ccgataaatt gttcggattg tcatatctgt
     1381 atttttccat ttttataatt cttaatattt catcaatcct agagtttatt tcttctcttt
     1441 caacacatag attttcggga ccgaatgcaa gttcatcttc aattgtagga gaaaatagct
     1501 gtgttgaagg attttgaaat attattccta tctttgtcac tacttccgcg atacttaaat
     1561 tttttatatt ttcaccgaaa atcaagacct ctccggttaa ttcccccttg tataatctcg
     1621 gcacaagccc gcagatacaa tggcacaagg tggttttgcc acttccgctt cgtcctgtta
     1681 ttgcaattgt ttcacctgtt cttacattga aatttacatc cttaaaaata tattctttcc
     1741 ggttgtcata tttaaagcat aaatttttaa cttcaactgc attcatcatt taacctcgat
     1801 ttcagtaatc cacttgcatc ttctgttgct gaaaaaatcc gaaactacta tggattcata
     1861 aggacctctt cctcccgagt ccctgctgcc aagatatctc ccgtcttcca taaaagcaat
     1921 atatacattt ttatcaatag aaacttcttc cgatgaataa gcaacagaat agccgtcggc
     1981 cgccttgatt ataataattt gtcctgtaaa tcaatattgt ggttattaag caagtctttc
     2041 agctgaacac ccctatatat atgcgttgac ggttcagtag tcgatgtgtc aagaacagct
     2101 tcaaagtcct caccgtaaag aattatttct tccttcgtta atactttctc tttgtcgtta
     2161 tatttcaata caatctgcgc attagataat atttgttttt gtttttcgat ggaggctttg
     2221 tttaagttta aagatattat taatgtaatt gtcaatatga ttactaataa tacaattatt
     2281 aaagctcttt ttttcatggc tctttaatga aataaggcac aaccatatct gattgacggc
     2341 atattccatg tcctcctcct tcggcagttt catgcatacc gtggtcgggc acaagtatta
     2401 ttgctccctc ccactcttca cttaagtctt ttatatattt atcgatagtt tttatataat
     2461 ccatcacttc tcttgaatat ggtccaaaac tgtggcctct gtcatcaatg ccgtgaaaat
     2521 gaataaaaat caaatcatag tcttcgttaa cggaaattaa tgcactttcg tatatttcat
     2581 catcggtctc gctgttttta ttaatatctg tatgaagttc aggctcaatt tcggtattta
     2641 atatttttat atctccttca agcaaaatac agcttttatt gttatcaaga gcatatttga
     2701 atatgctgtc tactttcatc cggcgattac ttctgtcatg aactccgttt acatcagggg
     2761 cttgtcccgt aatgattgct gcaaatcctg catttgtaac aggagtgtat acacttaagg
     2821 caggatattg gaaatatttg ctcaaaaaag taagagtatt ctcttcatta gcccttttaa
     2881 actgattgta ggaaaagccg tctaaaagaa ccgtcataac cttttcgcct ttatccaaat
     2941 attctttcat gtcataatat gcatttgtta tgctttgata attatcggtt aaataaattc
     3001 ctactgtatt ttcaactgtt tccccatcag ctgtaactgt attatttttt atgtagaatt
     3061 cggcaggcac ttgaataatt tctcctgtat ttaaaattac cgctgcttct gaaaatttat
     3121 cgtttgttat ttcgtttatt gaaatctctt tttcagactt aaaatcaacg aaaaaatttt
     3181 tattttcgtt gaatatattt atgcctgttg attctttata gtttacatca gcactgcatc
     3241 caaccaagat taccaaaaat attaataaaa atagtttaat cccaagacta tgttctgtat
     3301 aattaatagc cgacaatatt tttttcagct tcatttctaa tcctctaata ttttcatctt
     3361 ttccaaactt ctgttaatgg cgtaagctat aagcccgcct gttgctccgg acaaagctgc
     3421 tgcagacaag attaaaataa ccgttacaaa gggaagtctg aaaaatagta tattggaaat
     3481 aagcgtaccc gtaacatttg ccgccaaaca gccaaacaca tagtggagaa tattgtattt
     3541 tttatttatt gaaaacataa atattatatc tgtaacaata ccgggcagag tgtaagaaac
     3601 aatgctcatg atgccgtgag ttcccatagt tcccgttgca attaccaaaa ttccttggac
     3661 aatgcctatt aaggtacccg tgcctgattt tttgacaatt ccggcaccca agactatcca
     3721 aaacatatag aaaccgccgg ctattgctcc tcccggaata aacaagggtc ccgttataat
     3781 gtgaaccaag ggcaccacaa ttggttttgt tgcaattccg agtgccgata ccactgctat
     3841 gataattaaa ttataaactg aaaatttttc caaaaattta tttgtcataa aaacctac
//
LOCUS       DAOSOW010000160         3898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_23424_length_3898_cov_3.096539, whole genome shotgun sequence.
ACCESSION   DAOSOW010000160 DAOSOW010000000
VERSION     DAOSOW010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23424_length_3898_cov_3.096539"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /gene="truA"
                     /locus_tag="PLD67_05090"
     CDS             complement(<1..711)
                     /gene="truA"
                     /locus_tag="PLD67_05090"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOT21929.1"
                     /translation="MVRNIKLLIAYDGTAYHGWQTQLNKVTIQETIENALSIVMKQKI
                     DLTGSGRTDGGVHALGQVANFKADTKIPEDKIKIALNANLPPDIRILESVDVDMSFNA
                     RFDALDKTYMYQIYNDRVGNPFYSRYSCFIPQSLDIDKMKEALKLIKGTHDFKGFMAS
                     GSYVKTTVRTIYDTELIKEENLLKLYIKGDGFLYNMVRIIAGTLIDIGKGIKSIDCIE
                     DALINKNRKVLGQTAPPQG"
     gene            complement(705..1502)
                     /locus_tag="PLD67_05095"
     CDS             complement(705..1502)
                     /locus_tag="PLD67_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOT21930.1"
                     /translation="MFKNLTIGQHYPVDSPIHNLDPRLKIIMTFIFIISLFLIESFIG
                     FIFVIIFLTVIITISKVPVKFVIKGLRPILFIILITFIINLLMTPGRVIYTIGVIKIT
                     EEGLRQAGFMAVRLTLLIMGTSMLTLTTSPIILTDGIESLLKPFKRFGLPAHELAMMM
                     TIALRFIPTLMEETEKIMKAQKSRGADFESGNIMSRAKNLVPLLVPLFISAFRRADEL
                     AMAMEARCYRGGENRTRMRQMKISKGDYAAAFVFTVYFAAVLIIKIW"
     gene            complement(1495..2355)
                     /locus_tag="PLD67_05100"
     CDS             complement(1495..2355)
                     /locus_tag="PLD67_05100"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOT21931.1"
                     /translation="MSIRTENIKYIYSEGTPFRTLALDNVSVTINQGEFVGIIGHTGS
                     GKSTLIQLFNGLELPTEGKVEVDGTIVGKDKKELKLIRQTVGLVFQYPEYQLFEETVA
                     KDVAFGPMNLNISQQEIDFRVKEALELVGFNYNKIKDVSPFELSGGQKRRVAIAGVLA
                     MKPQYLILDEPTAGLDPAGRNEIFNQIKKLHEESKFTVVLVSHSMEDIAKLVDRVIVL
                     SKGKIHMEGSPKEIYSQAEELEKIGLGIPQTASIVRELKNRGFNIKQDILTIEEAKEE
                     ILKEVRRRNV"
     gene            complement(2346..3170)
                     /locus_tag="PLD67_05105"
     CDS             complement(2346..3170)
                     /locus_tag="PLD67_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HOT21932.1"
                     /translation="MIEYIIKIDNVKFKYDKNSSYAVNGVSLNIKSGEFTAIIGRNGS
                     GKSTLAKLINSILLPTEGKIFVKGMDTSDDSKLWDIRQTAGMVFQNPDNQLVATIIEE
                     DIAFGPENLGVQPEEIRRRVDNALNTVGMYEYRKRPPHLLSGGQKQRIAIAGILALNS
                     ECIILDEPTAMLDPSGRKEVMETLKKLNAEGKTILLITHYMDEAVQADRVIVMDKGNI
                     KLDGKPKEVFRNIDEIKKYGLDVPQVTELAQELIKEGLKLPPDIIDIKELVDILCQ"
     gene            3523..>3898
                     /locus_tag="PLD67_05110"
     CDS             3523..>3898
                     /locus_tag="PLD67_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21933.1"
                     /translation="MFCLRNKINICDFKPDIAQNLGNLSLTVRNRSSNEPVAFAEVSV
                     YYLTIYGVYGEGGEASMMVRHITDENGRIPVIELPVIDRINQPNNSYFVTVRHFRYYP
                     VNIMNIPIYPDITTEYNVMITPL"
BASE COUNT         1136 a          780 c          601 g         1381 t
ORIGIN      
        1 cccttgggga ggagccgtct gccctaatac tttcctgttt ttattaatta aagcatcttc
       61 tatgcaatca atgcttttta ttcctttccc aatatcaatt aaggttcctg caataattct
      121 taccatattg tacaaaaatc catcgccctt aatatagagt tttaacaaat tttcctcttt
      181 tataagctcg gtatcgtaaa tagttctcac cgttgttttt acataggagc ctgaagccat
      241 aaatcctttg aaatcatggg ttcccttaat caatttaagg gcttctttca ttttatcaat
      301 atccaaagac tgcgggataa aacatgaata ccttgaataa aacggattcc cgaccctatc
      361 attgtaaatt tgatacatat atgttttatc aagtgcatca aatcttgcat taaaactcat
      421 atcaacatca acagattcca atatccttat atccgggggc aaatttgcat taagcgcaat
      481 tttaatcttg tcttcgggta ttttggtgtc tgctttgaaa tttgcaacct gccccaaggc
      541 gtgaacgccg ccgtcagtcc ttcctgagcc cgttaaatca attttttgct tcattactat
      601 acttaatgca ttttcaatcg tttcttgaat tgtaacctta tttaattggg tttgccaccc
      661 gtgataagca gtaccgtcat aggcaattaa aagtttaata tttcttacca tatcttaatt
      721 attaatactg ctgcaaaata aaccgtaaaa acaaatgctg cagcataatc accctttgat
      781 attttcattt gcctcattct tgttctgttc tctcctccac gatagcatct tgcttccatc
      841 gccattgcca attcatcggc acgcctgaat gcacttatga aaagcggaac caagagagga
      901 actaaatttt ttgccctgct cataatattg ccgctttcaa aatcagcacc tcttgatttc
      961 tgtgccttca ttattttttc tgtttcttcc ataagggtag gaataaagcg aagagcaatt
     1021 gtcatcatca ttgccagttc atgtgcagga aggccgaatc ttttaaacgg ctttaacaga
     1081 ctttcgatac cgtcagtcaa tataatgggc gacgttgtca gtgtcagcat agatgttccc
     1141 attattagaa gagtaagtct tacagccata aatcctgcct gcctcaaacc ttcttcagta
     1201 atctttatta ctccgatggt atatataact cttcccggcg tcatcaaaag attgataata
     1261 aatgtaatta atatgatgaa taatatgggt cttaatcctt tgattacaaa tttaacgggt
     1321 acttttgaaa tagtaataat aacggtcaaa aatataataa caaatatgaa ccctataaag
     1381 ctttcaatca aaaataatga aattataaaa ataaatgtca tgattatttt aagcctgggg
     1441 tcaaggttgt gtatgggaga atcaacagga taatgctgtc cgatagttaa attcttaaac
     1501 atttcttctc ctcacttcct ttaatatttc ttcttttgct tcttcaattg tgagtatatc
     1561 ctgtttaata ttaaatcctc tattcttaag ctcccttacg atagaagctg tctgaggaat
     1621 tccaaggcct attttttcca attcttcagc ttgtgaataa atttcttttg gactgccctc
     1681 catatgtatt ttccccttgg ataaaactat aactctgtcc accagttttg ctatatcctc
     1741 catactgtgt gataccaaca caacggtaaa tttagactcc tcatgcaatt tttttatttg
     1801 attaaatatt tcattcctgc ctgcagggtc gagaccggca gtgggctcat ctaatataag
     1861 gtactgcggt ttcattgcaa gtacgcctgc aattgcaact cttctttttt gacctcccga
     1921 taactcgaag ggagatacat cttttatttt attataatta aaaccgacta attccaatgc
     1981 ctcttttacc cgaaaatcaa tttcttgctg ggagatattt aaattcattg gtccaaaggc
     2041 tacatccttt gcaactgttt cttcaaaaag ctggtattcg gggtattgaa atactaatcc
     2101 tactgtttga cgaatcaact ttaattcttt cttatctttg ccgactattg tgccgtcaac
     2161 ttcaacttta ccttcagtgg gaagctcgag gccgttaaaa agctgtatca aagttgattt
     2221 gccggagccg gtatgtccga ttattcccac aaattcgcct tggtttatcg taacgctcac
     2281 attatccaat gccaaggttc taaacggagt gccttcactg tatatatatt ttatgttttc
     2341 tgttcttatt gacataatat gtccaccaat tcctttatat ctattatatc aggaggcagt
     2401 ttcagccctt ctttaataag ttcctgtgct aactcggtta cctgcggaac atccaaaccg
     2461 tattttttaa tttcatcaat atttctgaat acttccttag gtttcccgtc caatttaata
     2521 tttcctttat ccataactat gactctgtct gcttggacag cttcgtccat ataatgtgta
     2581 attagaagta tggtcttgcc ttcagcgttt aattttttta atgtttccat aacttctttc
     2641 ctgcctgaag ggtctaacat tgcagtaggc tcatccaata ttatgcactc cgaatttaaa
     2701 gctaatatac ctgctatggc aattctttgt ttttgtcctc cagataagag gtgagggggt
     2761 ctttttctgt attcatacat gcctactgta tttaaggcat tatcaaccct tcttctaatt
     2821 tcttcaggtt gcacgcctaa attttcggga ccgaatgcaa tgtcttcctc aatgatggtt
     2881 gccactaact gattatcagg attttggaac accataccgg ctgtctgtct tatatcccaa
     2941 agtttagaat catctgaggt atccattcct ttaacaaaaa ttttcccttc ggtaggaagt
     3001 aaaattgaat ttattaattt agccagcgtt gatttcccag agccattgcg gcctataatt
     3061 gcagtaaact ctccgctttt tatattcagg gaaactccgt ttactgcata agaggaattt
     3121 ttatcatatt taaattttac attgtctatt ttgattatgt attctatcat tttacaccct
     3181 tatttgaatt tttgcgttaa aagctgcata agaataatat catcattcgc attattttac
     3241 aacagtaatt tacactaatg ttgttattgg ttcctttgga attacataaa aataaaaggc
     3301 tgagcaaagt atatcactct gcacgccttt tattttattc gtttaataat aattcatcat
     3361 cctgttttca cacaatattt atgataacaa aagagcgcct ttggaatttt ctaaaattat
     3421 tgtataatta tgcgcccact ccaaaagcac tgaacttgtt aatataatca ataattcata
     3481 attttcatat aataggttat aaccatatgg gaggaaatat aaatgttttg tttaagaaat
     3541 aaaataaata tatgtgactt taaacctgac attgcccaaa acttaggtaa tttatcctta
     3601 actgtgcgta acagatcaag caatgagcct gttgcttttg ctgaagtaag tgtttattat
     3661 ttgactatat atggagttta tggcgaggga ggagaagcaa gtatgatggt aaggcacata
     3721 acggatgaaa acggcagaat ccctgttatt gaactgcctg tcattgacag aatcaatcag
     3781 cctaataact cgtactttgt taccgtaagg catttcagat attaccctgt taatataatg
     3841 aatataccga tataccctga tattaccacc gaatacaatg taatgataac accgctga
//
LOCUS       DAOSOW010000161         3891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_23458_length_3891_cov_3.742440, whole genome shotgun sequence.
ACCESSION   DAOSOW010000161 DAOSOW010000000
VERSION     DAOSOW010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23458_length_3891_cov_3.742440"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..210
                     /locus_tag="PLD67_05115"
     tRNA            116..210
                     /locus_tag="PLD67_05115"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:150..152,aa:Sec,seq:tca)
     gene            228..596
                     /locus_tag="PLD67_05120"
     CDS             228..596
                     /locus_tag="PLD67_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOT21934.1"
                     /translation="MPNCVLVCVTRQKTSEKLIKKGVELIKGEAGSCLNVIHVVNEND
                     KFLYGLSDGDALEYLFEITKDVGAANLVVKRSKDVIKTIVEYAEEINCTHIVLGHSNN
                     PSNNFIAKLQRKLPNVEFII"
     gene            606..887
                     /locus_tag="PLD67_05125"
     CDS             606..887
                     /locus_tag="PLD67_05125"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HOT21935.1"
                     /translation="MGRILYIIKFLFDKNIPIREKRWVIIPLIYILSPADLIPFPILG
                     LGIVDDLVMLGFLLTIVYDKTKKYYFDKRHEDKDIIENVEYEIDEDEEK"
     gene            983..2383
                     /locus_tag="PLD67_05130"
     CDS             983..2383
                     /locus_tag="PLD67_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21936.1"
                     /translation="MNNNYKIIRTIYENAFQRILECKDDDTGAIFYSNIITSKKVINL
                     ININDLKKIKSNILECYNTEDRIYIFTKSLETDCRKLTDLANSNLTMKQQFNLAEKSI
                     NLASNIYNMTDVVQQKILDIDRLFIDDNNDLIVDCNLIFEQEYDIADNETFKRLGNII
                     HMIFSGIEIIDYNISDSIPPDILKIIVRCLTREYVFPNDALVELKRSPIFNMLFGEEI
                     INNTVDIEQPEEPSLVIHNEESDDYLNIYLEDNQPAKKKALINKDIKRAAVSLLTVVM
                     VLLLGNFIIKKFNKNDEAEAINNVKPPLQEAPSETPVEEEPVYESTDIHFSEELLESI
                     GYEGAKAQKDTDIYVEGSSSLTVANSGQDKVKALFAAVDFKDDNFNYMLMKQIGVSGK
                     IKSQYDTQARVVFEAYKDGVIKSNFHSLVNIYDDIWSQFTVPINVTEADVLYIYLEYE
                     GENKVWIDSLFIDVIK"
     gene            2510..3208
                     /locus_tag="PLD67_05135"
     CDS             2510..3208
                     /locus_tag="PLD67_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21937.1"
                     /translation="MDIIRMQGVSKIYKSGQIQVEALKNISFNIKEKEFVSIMGPSGS
                     GKSTLLNLVGCLDHPTSGTYELAGNQVDKLSDSKMSDVRNQFIGFVFQSFHLLPRMTA
                     IKNVEIPLIYRGMSDKKRRELAENALELVGLKDRMTHLPAQLSGGQQQRVAIARAIVG
                     NPSIILADEPTGALDSKSGELIMELFVELNEKNSMTVVQVTHEEFIAEYGSRIIRLVD
                     GQIVSDDVVEVKNV"
     gene            3201..>3891
                     /locus_tag="PLD67_05140"
     CDS             3201..>3891
                     /locus_tag="PLD67_05140"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOT21938.1"
                     /translation="MYKKIISIAIVLGLVFFGGYLTAKWLIPKDTEASAGPRYSTKAV
                     ERGDIKQGVNLSGQLSGNWGGSITAPRPEGITDSNGMSVSVTYTVEEVFVEPNQMIKK
                     GDNLVRLSAVNLGDILTELTDSIQKKQEDIQAKISNLEKILNKDVTDISQVNAYDGIV
                     FRAPIRGRITELSVEEGEKVENFNIATIVDDSMVKISFKVNTYEFESLKECQKVLLQY
                     RKTTEHGSQLAF"
BASE COUNT         1423 a          520 c          779 g         1169 t
ORIGIN      
        1 aaatgtcctc gtactgccca tactgctgca tattatgcag ctttttttaa ttcagtcatt
       61 acgatagtaa aatcagcttg aatttatcag gtggatatgg taatatattt gtgatgggga
      121 tagataggtg ctggtgtgcc tcctggtctt caaaatcagt tgcggggcgg aatcgtcctg
      181 ggtgggttca attcccacat attcccgcca aattaaggag ataaattatg cctaattgtg
      241 ttttggtatg tgtaacaaga cagaaaacaa gcgaaaaatt gataaaaaaa ggagtggaac
      301 tgataaaagg agaagctggc agctgtttaa atgttataca tgttgtaaat gaaaatgaca
      361 agtttttgta tggtttaagc gacggagatg cgttagaata tttatttgaa attactaaag
      421 atgtgggtgc tgctaatcta gttgttaaaa gatccaagga tgtaataaag acaatagtag
      481 aatatgcgga agaaataaat tgcactcata ttgtattggg ccactcgaac aatccatcaa
      541 acaattttat cgctaagctg cagaggaagc ttcctaatgt tgagttcata atctaggatg
      601 cgaatatggg cagaatttta tatataataa aatttttatt tgataaaaat attcccataa
      661 gggaaaagag gtgggttatt atacctctta tttacatatt gagccctgcg gatttgatac
      721 cttttccaat attggggctc ggaatagtag acgatttggt aatgcttgga tttttattaa
      781 caatagtcta tgataaaacc aagaaatatt attttgataa aagacatgaa gacaaagata
      841 ttatagaaaa tgttgaatat gaaattgacg aggatgagga gaaataactc atccttattt
      901 atttgtaaaa taatacaaaa atgtctttat ttttgtcgaa atacttgtta atatatagtt
      961 aataatattt ggaggtctgt atatgaataa taattacaag attatccgta caatttatga
     1021 aaatgctttt caaagaattt tagaatgcaa agacgatgat acgggtgcaa ttttctactc
     1081 caatataata acaagcaaaa aggttataaa tttaataaac ataaacgatt taaaaaagat
     1141 taaatccaat atattagagt gctacaatac agaggataga atttatatat ttacaaaatc
     1201 tttagaaaca gattgcagaa agttaacaga tttagcaaac agtaacctca ccatgaaaca
     1261 acagttcaat cttgcagaaa agtcaataaa tttagcctca aatatttaca atatgaccga
     1321 tgtagtacag cagaaaattt tggatataga ccgattgttc atcgatgata ataatgattt
     1381 gattgttgac tgcaatctga tatttgagca ggaatatgat atagcagaca atgaaacatt
     1441 taaaagattg ggcaatatta tacatatgat tttttccgga atagaaatta ttgattataa
     1501 catatccgac tcaatacctc ctgacatatt gaaaataata gtaagatgcc ttacaagaga
     1561 atatgttttt ccaaacgatg ctttggtgga attaaaaagg tcgcctattt ttaatatgct
     1621 ttttggagaa gaaattatta ataatactgt ggatattgaa caacctgagg aaccgtctct
     1681 tgtaatacac aatgaagaat cggacgatta tctcaatatt tatttggaag ataatcaacc
     1741 agctaaaaag aaagcactta taaataaaga tatcaaaaga gccgctgtat cattgcttac
     1801 agttgttatg gtacttcttt tgggtaattt tataataaaa aaattcaata aaaatgatga
     1861 ggccgaagca attaacaacg ttaaaccacc tttgcaggag gcaccttcag aaacaccggt
     1921 tgaagaagaa cctgtttacg agtcaacaga tattcatttc agtgaagagc tgttggagag
     1981 catcggatat gaaggagcaa aagcacaaaa agataccgat atatatgtgg aaggaagctc
     2041 ttccttaaca gtggccaata gtggacagga taaagttaaa gcactgtttg cagctgttga
     2101 tttcaaagat gataatttca actatatgct tatgaaacaa ataggggttt caggaaaaat
     2161 caaatcacaa tatgatactc aagctcgggt agtttttgaa gcatataaag atggtgttat
     2221 aaagtcaaat tttcattcat tggttaatat atatgatgat atatggtcac agtttacagt
     2281 acctattaat gtaacggaag ctgatgtctt gtatatttat ttggaatatg agggtgaaaa
     2341 taaagtgtgg attgattcct tgtttattga cgtaatcaaa taattaatca aactgactat
     2401 tggaacgacc cggtcgttct tttttttaca aaattattaa tgtttactga actttttctt
     2461 aagtttatac gtctaatata tggactgatg tgtaaaagag aggtggaaga tggatataat
     2521 cagaatgcaa ggtgtttcaa aaatttataa atcaggtcaa attcaagtgg aagctttaaa
     2581 aaacatttcc ttcaatatta aagaaaaaga atttgtttca attatgggac cttccggctc
     2641 gggaaaatca acactgttaa acttagtggg atgcttagac catcctacaa gcggaacata
     2701 tgaattagca ggaaatcagg ttgacaaatt aagcgattct aaaatgtcag atgtaaggaa
     2761 ccaatttatc ggatttgtat ttcaaagctt tcatttgctg ccaaggatga ctgcaataaa
     2821 aaatgttgaa attcctttaa tatatagggg aatgagcgat aaaaaacgaa gagagctggc
     2881 cgaaaatgca ttggagctgg taggactgaa ggacagaatg acacatttgc ctgctcaatt
     2941 gtcgggcggc cagcagcaaa gggtagctat agccagggct atcgtaggaa atccgtcaat
     3001 tatattagcg gatgagccta caggagcatt agattcaaaa tcaggcgaat tgattatgga
     3061 attatttgta gaattaaatg aaaaaaactc aatgaccgtc gttcaggtaa ctcacgaaga
     3121 atttattgca gaatacggct caagaatcat aagacttgtg gatggtcaaa tagtatctga
     3181 tgatgtagtg gaggttaaaa atgtataaaa aaattatatc gatagcaata gtattaggat
     3241 tagtattttt cggcggatat ttaacggcaa agtggcttat tcctaaagat acggaagcat
     3301 ctgcaggtcc aagatattca acaaaggcag ttgaaagagg agatataaaa caaggagtta
     3361 atttatcagg gcagcttagc ggtaactggg gaggctcaat aacagcacca agacctgaag
     3421 gtataactga ttcaaacggt atgagcgtat cggtaacata tactgttgaa gaagtgtttg
     3481 ttgagcctaa ccaaatgatt aaaaagggag ataatttagt tcgtttatct gccgtaaact
     3541 taggtgacat tttgactgaa ttgaccgaca gcattcaaaa gaaacaggaa gatattcagg
     3601 caaaaatatc taacttagaa aaaatattaa ataaggatgt tacagatata agtcaggtta
     3661 atgcttatga tggaatagta tttagagcac ctattcgagg acgaattaca gaactttcgg
     3721 ttgaagaggg tgaaaaggta gaaaacttca atattgctac tattgttgat gacagtatgg
     3781 tgaaaatttc tttcaaagtt aatacatatg agtttgaaag tttgaaggaa tgtcaaaaag
     3841 tattgctgca gtatagaaag acaacggaac atggctccca gcttgctttt g
//
LOCUS       DAOSOW010000162         3885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_23500_length_3885_cov_3.286162, whole genome shotgun sequence.
ACCESSION   DAOSOW010000162 DAOSOW010000000
VERSION     DAOSOW010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23500_length_3885_cov_3.286162"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..896)
                     /gene="murI"
                     /locus_tag="PLD67_05145"
     CDS             complement(90..896)
                     /gene="murI"
                     /locus_tag="PLD67_05145"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005583676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOT21939.1"
                     /translation="MDNRPIGIFDSGIGGLTVMREVMEQLPYENIIYLGDTARIPYGS
                     KSEQTIKKFSLQCATFLKNKDVKTIVIACNTASSNALDLLTEEFDIPVIGVIDPGARS
                     ASKATKTGKIGVIGTTATINSYAYQKKIMEYMNNAEVIGIPCPLFVPIVEEGWEYSQV
                     AELTAEKYLTELIEHDVDALVLGCTHYPILRHTIKKVLGPQVTLVNPAFETAKDLKNL
                     LIEKDILNENYDKPRYEYYATDAPERFRRIGGNFLKKEIDNLYEIAIDNL"
     gene            1320..2357
                     /locus_tag="PLD67_05150"
     CDS             1320..2357
                     /locus_tag="PLD67_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-erythro-3,5-diaminohexanoate dehydrogenase"
                     /protein_id="HOT21940.1"
                     /translation="MKKGNPYGTHRVIEPKGVLPQPALKIDNTMEIFDNEILIDVQTL
                     NIDSASFTQISDQAGGDVEKIKEIMKGIVAERGKHQNPVTGSGGMLIGTIEQIGPALE
                     GKTDLKVGDKIATLVSLSLTPLVIDEILEVRKDIDQVDIKGKAILFESGIYAKIPEDI
                     PENLALSVLDVAGAPAQTRKLVKEGDTVLVLGGGGKSGLLCLYEAKKKAGPTGKVICW
                     AHREVSLNTVRELGLADVLISGDATNATEVYEKIMEATDGQLCDLVISVVSRPNCEMS
                     AILATKDEGTVYFFSMATSFTKAALGAEGVGKDINMIIGNGYTKGHSETALQIMRESK
                     GLRELFTKLYA"
     gene            2438..3688
                     /gene="ablA"
                     /locus_tag="PLD67_05155"
     CDS             2438..3688
                     /gene="ablA"
                     /locus_tag="PLD67_05155"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOT21941.1"
                     /translation="MAYYNEIELWKDVKPQEWNDWRWQVENRIDTIEKLKKIINLTAQ
                     EEEDISKVLEKFRMGITPYYAAHMDKDDPRDPIRMQAVPTFVETHVSAADMVDPLHED
                     TDSPAPGLTHRYPDRVLFLITDQCSMYCRHCTRRRFAGQSDDEVGMENIDKCIEYIRK
                     TPQVRDVLLSGGDCLLVSDKKLEYIISKLREIPHVEIVRLGSRTPVVLPQRITDDLVN
                     MLKKYHPIWLNTHFNHPKEFTPESMEALRKLADAGIPLGNQSVLLRGVNDCPHIMRDL
                     VHKLVMNRVRPYYIYQCDLSLGIEHFRTPVAKGIEIMESLRGHTSGFCVPTFVVDAPG
                     GGGKTPVGPNYVLSQSADKVILRNYEGVITTYTEPKLPELECRCDVCQGKKTDPLTGV
                     AGLLQGNDLALEPTKLARRERTHH"
BASE COUNT         1290 a          681 c          793 g         1121 t
ORIGIN      
        1 aatcagctca ataacatctt cgttgcctgc ttcatcaatt gtctgtattg tgtttattct
       61 gactttcact ttttccattt tatatcccct taaaggttat ctatggcaat ttcataaaga
      121 ttatcaattt ctttttttaa aaagttgcct cctattcttc taaaacgctc cggtgcatca
      181 gttgcataat actcatatcg cggtttatca taattttcat tgaggatatc tttttctatc
      241 aaaaggtttt ttaagtcctt ggcagtttca aaagcaggat ttacaagtgt tacttgaggc
      301 cctaaaactt tttttatagt atgcctcagt ataggataat gggtacatcc taaaaccaaa
      361 gcatcaacat catgctcgat taattccgtc aagtattttt ccgcagtaag ctctgccact
      421 tgagagtact cccagccttc ttcaacaatt ggtacaaaaa gcggacaagg tatgccgatt
      481 acttcggcgt tattcatata ttccattatt tttttctggt atgcatagga gttgatggtg
      541 gctgtagtgc ctattactcc tattttgccg gttttggtag cttttgaagc actccttgca
      601 ccggggtcta ttactccgat aactggaata tcaaactcct cggtcaataa gtcaagcgca
      661 tttgagcttg ctgtattaca tgctataaca atggttttta catctttatt ttttaaaaat
      721 gtagcgcatt gaagtgaaaa tttctttatt gtttgctcag atttagagcc gtagggtatt
      781 cttgcggtat ctcctaaata tattatgttt tcgtagggaa gctgctccat aacttctctc
      841 attactgtca gtcctcctat tccggagtcg aaaattccaa ttggtctgtt atccatttta
      901 taccatccta tacttattaa atttattttt ttactatatt ttattaaaag attgttatat
      961 gatgtatgat attcataatc atttgatatt ttactatgct aagaattatc atatcgtctc
     1021 aggccatgaa tacgctttaa tgcggcttgc ttcacaccga tacaagaaat tcaaatcata
     1081 taaaaccatt ttggttttat caccttttga atttattata tcataatatt atttactcat
     1141 caacaaaaag ctgaattttc ggcacttacc tgacaatgaa aacattttta taaagcaaat
     1201 gttgaaaact ggctgttttt tatttttgga taattggcat aaattttgct aattatatat
     1261 ttgaatatac ttaatattcc atacaatcca tacccaatta ttgaaaattt ggaggcaata
     1321 tgaaaaaagg aaacccatac ggcacacaca gagtgattga acctaaagga gtgttaccac
     1381 aaccggcttt gaaaattgac aacacaatgg aaatatttga caacgaaatt cttattgatg
     1441 tgcagacatt gaatattgac tctgctagct tcacgcaaat cagcgatcaa gcaggcgggg
     1501 atgtagaaaa aatcaaagaa attatgaaag gtatcgttgc cgaaagagga aaacatcaaa
     1561 accctgtaac aggttcaggc ggaatgttaa tcggtactat tgagcaaata ggaccagcgc
     1621 ttgagggaaa aacagatttg aaggttggag ataaaatagc aacactggta tcattgtcgc
     1681 tgactccatt agtaattgat gaaatcttag aagttaggaa agatattgac caagttgata
     1741 tcaaagggaa ggcaatattg tttgaaagcg gaatttatgc aaagattcct gaagacatcc
     1801 ccgaaaatct tgctttgtca gtgcttgatg tagcaggagc accggcgcaa accaggaaat
     1861 tggtcaaaga aggagatacc gtattagttc tcggcggggg aggcaaatca ggtcttttat
     1921 gcttatatga agcaaagaag aaggcaggtc ccacaggaaa agttatttgc tgggcccaca
     1981 gggaagtatc actgaataca gtaagagaac ttggattggc agatgtttta atatcaggcg
     2041 atgctacaaa tgcaactgaa gtatacgaaa agattatgga agcaacagac ggacaattat
     2101 gtgatctggt aataagcgta gtatcaagac caaattgtga aatgtcggca atacttgcta
     2161 caaaagacga agggacagta tatttcttca gcatggctac aagctttacg aaagcagcat
     2221 taggagccga aggtgtaggc aaggacataa acatgataat cggtaacggc tatacaaagg
     2281 gacattcaga aacggccctg cagataatga gggaaagcaa aggattaaga gaattattca
     2341 caaaattata tgcataaata cataataaac taagcaacat gtcatttgct cggcgaatga
     2401 catatgttgc ttgtttaaat aattgaggag gtagaatatg gcttattaca atgaaataga
     2461 gctatggaag gatgttaagc cacaagagtg gaacgactgg agatggcagg ttgaaaacag
     2521 aatagacaca atagagaaac taaagaaaat aattaatctt actgcgcaag aggaagaaga
     2581 tataagcaag gtgttggaaa aattcagaat gggtatcaca ccatattatg cagcacatat
     2641 ggataaagat gacccgagag accccataag aatgcaggct gttccaacat ttgttgaaac
     2701 acatgtaagc gctgcagata tggttgaccc tttgcatgaa gatacggatt cgcctgcgcc
     2761 gggattaacc catcgctatc cggacagggt tttgttctta ataaccgacc aatgctcaat
     2821 gtattgcaga cactgtacaa gaagaagatt tgcaggacaa tcggatgatg aagtaggaat
     2881 ggaaaatatc gataaatgca tcgaatatat cagaaagact ccccaagtaa gggacgtatt
     2941 gctgtcaggc ggagactgtt tgttagtgtc agacaaaaaa ttagaatata ttatcagtaa
     3001 attaagagaa ataccacatg ttgaaattgt gcgtttaggc tcaagaacac ctgtagtctt
     3061 gcctcagcgt attacggatg acctggtaaa tatgcttaaa aaatatcatc cgatttggtt
     3121 aaacactcat tttaaccatc cgaaagaatt tactcccgaa tctatggaag ctttaagaaa
     3181 gttggctgat gcaggtattc ctttaggaaa ccaatcagta ttgttaagag gagtcaatga
     3241 ctgcccgcat attatgagag atttggttca taagcttgtt atgaacagag taagacctta
     3301 ctatatttat caatgcgacc tttcactcgg tatcgaacac ttcagaacac ctgtggcaaa
     3361 gggaattgaa ataatggaaa gcttgcgagg acatacttca ggtttctgcg tacctacctt
     3421 tgttgttgat gcaccggggg gcggaggaaa aacacctgta ggaccgaatt atgtattgtc
     3481 acaatcagct gacaaggtaa ttttaagaaa ctatgaaggt gttattacaa catatacaga
     3541 gccgaaatta ccggaattgg aatgtcgctg cgatgtatgt caaggcaaaa aaacagatcc
     3601 cttaacagga gttgcaggac tactgcaagg aaatgactta gcacttgaac ctacaaagtt
     3661 ggcaagaaga gaaagaacac atcattaaag atgccctaac cctatttttc cgaccagagg
     3721 gagagaaaaa tagcggtagg tcattcgcaa cgaattccca atgtatgcga ccaaagggag
     3781 caaatacatt ggtgaatgag actgcgagat gccctaaccg gcgccaattt tatgcgacca
     3841 aagggagcaa atacattggt gaatgagact gcgagatgcc ctaac
//
LOCUS       DAOSOW010000163         3864 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_23662_length_3864_cov_4.263061, whole genome shotgun sequence.
ACCESSION   DAOSOW010000163 DAOSOW010000000
VERSION     DAOSOW010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23662_length_3864_cov_4.263061"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1242
                     /locus_tag="PLD67_05160"
     CDS             <1..1242
                     /locus_tag="PLD67_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOT21942.1"
                     /translation="KVTQENIEETVNWFEENIRENDLPSDGLVLTYDDIKYGESLGST
                     AKFPKDSIAFKWSDEIKETTLLEIEWSASRTGLINPIAVFEPVELEGTTVSRASVHNL
                     SIMEDLELGIGDRINVYKANMIIPQISDNLTRSNNIEIPEHCPVCGGETSIKNDNDIK
                     TLYCINDECLAKQIKSFTHFVSRDALNIKGLSEATIEKLIAKGLIKELADIFHIERYK
                     DIITEMEGFGEKSFNNLVNSVNKARKTTAARLLYSFGITNIGLSNAKLISRKFGDDWE
                     KIQNAKYEELIEISGIGEVMAKNYVKFFSDEKKKVIVEDVLKEVEIETSSKTSSTQIF
                     ENYNFAITGAVEHFKNRDELKAAIEERGGKVTSSVTSKTNYLINNDNLSNSSKNKKAK
                     ELGIEIITEEQFLKWMNTGVR"
     gene            1265..1894
                     /locus_tag="PLD67_05165"
     CDS             1265..1894
                     /locus_tag="PLD67_05165"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOT21943.1"
                     /translation="MTKIYLTRHGETVWNRQCRFQGHKNSDLTEKGILAAELLADRIE
                     EIELDYIVSSPLMRAYNTAEIIRGNKNIQIIKHDGLKEINLGKFEGMSYKDIQKNYSK
                     LLAEIEKDPFNNRYPNGENLQEFYKRVVKAFNEVIDKYRNKSTLIVAHGGTIKCIEAY
                     IRKFKLSSDWMGNVVKNCSLSYLEIDDNNEIKEIFYNDTKHLEGSAALN"
     gene            1964..2788
                     /locus_tag="PLD67_05170"
     CDS             1964..2788
                     /locus_tag="PLD67_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOT21944.1"
                     /translation="MRKFKVALIQTMVYDDKEKNVKNAVKMIGKVSKEGADIAVLPEM
                     FCCPYNTSYFREYGEEEGGYAYKELSKAANSSGIYVVAGTIPELERTHVYNTSYVFDR
                     KGKRVAKHRKMHLFDIDVEGGQYFKESDVLSPGRDVTVFDTEFCKIGLGICYDIRFPE
                     LSRLMVLEGAEVIIFPAAFNMTTGPAHWELNFRGRALDNQVYTVGVAPARDMESSYHS
                     YGNSIVVSPWGNVINRMDEKEGYIIQEIDLEYVKKVRNELPLLKHLRRDLYTLSKN"
     gene            2856..>3864
                     /locus_tag="PLD67_05175"
     CDS             2856..>3864
                     /locus_tag="PLD67_05175"
                     /inference="COORDINATES: protein motif:HMM:NF018682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HOT21945.1"
                     /translation="MNENLLSGIYKNNEEMNVNFEEQGKKIVILDDDPTGTQTVYDLP
                     IYTRVDYEIIQKIFQDNNIKAFYILTNTRSMSKEKVMELDSKIARNIDLVSKRTGIDY
                     ELIVRGDSTLRGHFPLELNIIKDNISKKIDGYFLIPFFQEGGRITVDDVHYVKENNTF
                     IPVGETQFARDHTFGFTSSNLKDYVEEKTNCLVLKDQVVSISLEELRSNSSAVFNKIM
                     DLNGRICIVNAENYSDIQAFVNGLIKAEYQGKNFLFRTAASFVKSRIGLKDNGLLDGN
                     TLNIIDNRGGLIVAGSYVNKTSQQINHLMKETSIYSIEIDVLSILTESDMDIYAVSGP
                     NK"
BASE COUNT         1505 a          500 c          784 g         1075 t
ORIGIN      
        1 aaagtaactc aagaaaatat tgaagaaaca gtaaattggt ttgaggagaa cattagagaa
       61 aacgacctgc cttcggacgg acttgttcta acatatgatg atataaaata cggagaatca
      121 ttaggaagta ccgcaaaatt tccgaaagat tcaattgcct ttaaatggag tgacgaaatt
      181 aaggaaacta ctcttttgga aattgaatgg agtgcctcaa ggacgggatt aataaatcca
      241 atcgcagtat ttgagcccgt ggagcttgaa ggaacaaccg taagccgtgc cagtgttcat
      301 aatctaagca ttatggaaga cctggagctt ggaatcggcg atagaatcaa tgtgtataag
      361 gcaaatatga taattcctca aatttcggat aatttaacca ggagcaataa tattgagatt
      421 ccagaacatt gccctgtatg cggcggtgaa acatcaatta aaaatgataa tgacattaag
      481 acattgtact gcataaatga cgaatgttta gcaaagcaaa taaaatcatt cacacatttt
      541 gtaagcagag atgcattaaa tattaaagga ctttccgaag ccactattga aaaattaatt
      601 gcaaagggat tgattaaaga attagctgat atttttcata ttgagagata taaagatata
      661 attaccgaga tggaaggatt tggcgagaaa tctttcaata atctggtcaa ttcagtgaat
      721 aaggcgcgaa aaacaacagc agcgaggctt ttgtacagct ttggaattac caatataggt
      781 ttgtcaaatg ccaaattaat cagcaggaag ttcggcgatg actgggagaa aattcaaaat
      841 gcaaaatatg aagaactgat tgaaataagc ggcatcggag aagtgatggc taaaaattac
      901 gtaaaatttt tcagtgatga aaagaaaaaa gttattgtag aagatgtgct taaagaagtg
      961 gagatagaaa caagctctaa gacatcatca acacaaattt ttgaaaatta taattttgca
     1021 attacagggg ctgttgagca tttcaaaaac agggatgaat taaaagctgc aattgaagaa
     1081 agaggaggaa aggttacctc ttctgtgact tcaaaaacaa actatttaat caataacgat
     1141 aatttatcaa attcttccaa gaataaaaaa gctaaagaat taggaataga aattataacg
     1201 gaagagcaat ttcttaagtg gatgaataca ggtgtaagat agacgagata gaaagggaat
     1261 tgatatgaca aaaatatatt taacgagaca tggcgaaaca gtctggaaca gacaatgcag
     1321 gtttcaagga cataaaaatt cggatttaac ggaaaaggga atattggcag cagaattatt
     1381 ggcagacaga attgaagaga tagagctgga ttatatagtt tcaagccctt tgatgagagc
     1441 ttacaatact gctgaaataa ttcgcgggaa taagaatata caaattataa aacatgatgg
     1501 tttaaaagaa ataaatttag gcaaattcga aggaatgagc tataaggata tccaaaaaaa
     1561 ttattctaag ctgcttgccg aaattgaaaa ggaccctttc aacaaccgtt atccaaacgg
     1621 agaaaacctt caggaatttt acaaaagggt tgtgaaggca tttaatgaag taatagataa
     1681 atacagaaac aagtcaacct taatagttgc ccatggaggt accattaaat gtatcgaagc
     1741 ttatataaga aaatttaagt taagcagtga ctggatggga aatgtagtta agaattgcag
     1801 cctttcttat ttagaaatag atgataataa tgaaataaaa gaaatattct ataatgatac
     1861 aaaacatttg gaaggaagcg cagcacttaa ttaattgtcg aaatttcccg ggaaaaacag
     1921 aataaggtag aaaattggca agtatttata agaggagggt gctatgagaa aattcaaagt
     1981 tgcattaatt caaaccatgg tttatgacga taaagaaaaa aatgttaaaa atgctgtgaa
     2041 aatgattggt aaagtgtcaa aggaaggcgc cgatattgca gtgttaccgg agatgttctg
     2101 ctgcccttat aacacttcat attttagaga atatggtgaa gaagaaggcg ggtatgctta
     2161 taaagaatta agtaaagcag caaacagttc aggtatttat gtcgttgccg gaacaatacc
     2221 cgagcttgaa agaactcatg tttacaatac atcatatgtg tttgacagaa aaggcaaaag
     2281 agttgccaag cacaggaaaa tgcatttatt cgatattgat gttgaaggag gacaatattt
     2341 caaagaatcg gatgttctct ctcccggaag agacgtaaca gtctttgata cggaattttg
     2401 caaaatcggt ttaggtatat gttatgatat aagatttcct gaattaagca ggctcatggt
     2461 attggaagga gctgaggtta ttatttttcc tgccgcattt aacatgacca ctggtcctgc
     2521 tcattgggag ttaaatttta gaggaagagc ccttgataac caggtgtaca cagtaggagt
     2581 tgctcctgcc agagatatgg aatcatccta tcactcctat ggtaattcca tagtagtctc
     2641 accatggggc aatgtgataa acagaatgga tgaaaaagaa gggtatataa tacaggaaat
     2701 tgaccttgaa tatgtaaaga aggtaagaaa tgaactgcct ctattaaagc atttaagaag
     2761 agacttatat acattaagca aaaactaatt atattgtaaa ttaatggttt gggcaattta
     2821 ctacagacta tttatctgtg gaattggaga ttgttatgaa tgaaaattta ttgtcaggaa
     2881 tttataaaaa taacgaagaa atgaatgtaa actttgaaga gcaaggtaaa aagatagtaa
     2941 ttttagatga tgatccgaca ggtacccaaa cggtttatga tttacctata tatacaagag
     3001 tagattatga aataattcaa aaaatatttc aggataataa tattaaagca ttctatattt
     3061 taactaatac cagatcaatg tcaaaagaaa aagtaatgga actcgactcc aaaattgcca
     3121 ggaatattga tttggtatct aagagaacag gtatagatta tgaattaatt gtaagaggag
     3181 actcaactct cagaggacat ttcccactag aattaaatat aataaaggat aatatatcaa
     3241 aaaaaataga tggatatttt ttgataccat tttttcaaga aggcgggaga attactgttg
     3301 atgatgtcca ttatgttaaa gaaaataata catttattcc tgtaggagaa acacagtttg
     3361 caagagatca tacatttggt tttacttcat caaacttaaa agattatgta gaagaaaaaa
     3421 caaattgtct agtattaaaa gaccaagttg tatcaataag tttagaagaa ttaagaagca
     3481 acagcagtgc agtttttaat aaaataatgg atttgaacgg aagaatatgt attgttaatg
     3541 cagaaaacta tagtgatatt caagcatttg taaacggact cataaaagcc gaatatcaag
     3601 ggaaaaattt cctctttaga acagctgctt cttttgttaa gtcaaggatt ggtttaaagg
     3661 ataatggttt gttagatggg aatacgttaa acataattga caacagagga ggtttgatag
     3721 ttgccgggtc ctatgtaaac aaaactagtc aacagattaa tcatctgatg aaagagactt
     3781 ctatttacag catagaaatt gatgttctgt ctattcttac agaatcagat atggatatat
     3841 atgctgttag cggacctaat aaaa
//
LOCUS       DAOSOW010000164         3860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_23698_length_3860_cov_4.248357, whole genome shotgun sequence.
ACCESSION   DAOSOW010000164 DAOSOW010000000
VERSION     DAOSOW010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23698_length_3860_cov_4.248357"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3398
                     /gene="mfd"
                     /locus_tag="PLD67_05180"
     CDS             <1..3398
                     /gene="mfd"
                     /locus_tag="PLD67_05180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOT21946.1"
                     /translation="NLLDTSDKILILTSSEAKSKKYEEIIRAYTEHSERLQPKEFILY
                     NVDALSKDVEYKRANLLNNILNKRKCIVTASINSIITRVMSKDRFKESIIELKYGDSY
                     DLNELRSNLIKLKYERVDAIEGVGQFSVRGGIIDIFSPSEANPFRVEFFDDEVDSIRL
                     IDLKTQRSVKNIKSAKILPCSDILFKKEEIKIILSEIEKDYDKQSQNQSLSKEAFKNL
                     KNLYKSYQDKLMGGMGIENADLLIPFIKESFSNIIDYFEDDSILLLDEPERIFEELND
                     LNESFQLKYTELFERGEIFSNQSEVYISEHELLTKISKKPFISINGKEKIIKTDDSVH
                     MLFKEAPSYYGKIEELSKDLNRLKYKGYKVDIILSTYESCRKLYNLLNDYECTTTLSK
                     DTNAAESGQVVIAPGELTKGFEYYDNKLLVLTENEVLGSVKRKSRKTKKRKGAAIEIF
                     TDLKVGDYVVHEHHGIGQYIGIEKIEVQNIQKDYLCIKYKGEDKLYVPVDQMSLIQKY
                     IGTDSEKPKLNKMGSSDWIKTKERSKAAIENMASELIKLYAERKVLKGYAFSKDTEWQ
                     KEFEYKFPFEETEDQLRCVKEIKKDMEKPICMDRLLCGDVGFGKTEVAMRAAFKAVMD
                     SKQVAMLVPTTILAQQHYTNLVERFRGYPIKIEMLSRFRTHYQQNKIINDLNKGLIDI
                     VIGTHKLLSKELKFKDLGLLIIDEEQRFGVRHKELIKQLKSNIDVLTLSATPIPRTLH
                     MSMIGVRDMSIISEPPGDRLPIQTYVIEYNEGLIKDAIEKEISRKGQVYYVHNRVADI
                     DQTAAKLRKLLPEARIAVAHGQMSERHLENIMLEFVNKEYDILVCTTIIETGMDIPNV
                     NTLIIDNADYLGLSQLYQLRGRIGRSNKVAFAYLTYEKDKMLSEVADKRLKAIKEFTE
                     FGSGFKIAMRDLEIRGSGNILGPEQHGHMLAIGYDLYVKFLDRAVKEIQGKQVEEDIE
                     TSVDLNVDGYIPSSFIENEEQKIEIYKKIAAAENKDDIFDITEEIIDRFGNIPVQVDN
                     LLKISYIKSLSKRIKIKSIAQAGNTVNIEMTKDYLSQDIIEFLIKNYYEKISFVGTNE
                     PIIKYKLDSLEQMKILRELEEFLETLNNFKAGKTAVEEKHEQI"
     gene            3385..>3860
                     /locus_tag="PLD67_05185"
     CDS             3385..>3860
                     /locus_tag="PLD67_05185"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOT21947.1"
                     /translation="MNKFKKSLFLITLLCIALVFTACSNSGGKDVDKEGVMATVNGKP
                     IMQKDYDDALAYYKDYVEYQLGEDAWETETPRGTYKEIYEEYVWDEMTKSLLLLDAAE
                     KEGIKASEEEVQHSLENFKINFENDEEYKSFMEQRGITEDFIIQELTKEILINNYIT"
BASE COUNT         1491 a          529 c          774 g         1066 t
ORIGIN      
        1 gcaatctttt agatacatcc gataaaatat taattctaac aagcagtgaa gcaaaatcga
       61 aaaagtacga agagattatt agagcttata cagagcattc agaacgcctt cagcctaaag
      121 aatttatttt gtataatgtt gatgctttaa gcaaggatgt ggagtataaa agggcaaatc
      181 tgttaaataa cattttaaat aagcggaaat gtattgtaac tgcttcaatt aattcaataa
      241 ttacaagagt aatgtctaaa gatagattta aggaaagcat tatagaatta aaatatggcg
      301 acagttatga tttaaatgaa ttaagaagca atttgattaa gcttaaatat gagagagtgg
      361 atgccatcga aggggttggc caattttctg taagaggcgg aattattgat attttttctc
      421 cttcagaagc aaatccgttc agggtagaat tttttgatga tgaagtagat tcaataagat
      481 taatcgattt aaaaacacag cgctcggtta agaacattaa atctgctaag attcttcctt
      541 gcagcgacat cttgtttaaa aaagaagaaa taaaaattat tttatctgaa attgaaaagg
      601 attatgataa gcagagtcaa aaccaaagct taagcaaaga agcttttaag aatttaaaga
      661 acctttacaa gagctatcaa gataagctta tgggcggtat gggtattgaa aatgccgacc
      721 ttttgatacc atttataaag gaatcttttt caaatattat agattatttt gaagatgatt
      781 ctattcttct tcttgatgag cctgaaagaa tatttgaaga acttaatgat ttaaatgaaa
      841 gctttcagct gaaatatact gaattatttg aaagaggaga aattttttca aatcaaagtg
      901 aagtatatat aagcgagcat gaactattga cgaagatttc caaaaaacct ttcatttcta
      961 taaacggtaa agagaaaata attaaaacag atgattctgt tcatatgctt tttaaagaag
     1021 ctccttctta ctacggcaag attgaagagc tgtcaaaaga tttaaacagg ctgaaataca
     1081 agggctataa agtagatatt atactaagca cctatgaaag ctgcaggaaa ctttataatt
     1141 tattaaatga ttatgaatgc acaaccacat tgtctaaaga taccaacgca gcagaaagcg
     1201 gacaagtggt aattgcaccg ggagaattaa caaaaggatt tgaatactac gacaataagc
     1261 tccttgtgct tacagaaaat gaggtgcttg gctctgtcaa gcgcaaatcc cgcaagacga
     1321 aaaaacgcaa gggtgctgca attgaaattt tcaccgattt aaaagtagga gattatgtag
     1381 tacatgagca tcatggaatc ggtcaatata taggaattga aaaaatagaa gtacagaata
     1441 ttcaaaagga ttatttgtgc atcaagtata aaggtgaaga caagttatat gttcctgttg
     1501 accaaatgtc tcttatacaa aagtatatcg gaacagattc cgaaaaaccc aagcttaaca
     1561 aaatgggcag ctcagactgg ataaaaacca aggagcgctc aaaggcagcc atagaaaata
     1621 tggcaagtga gctgattaag ctgtatgctg aaagaaaggt attaaaggga tatgcttttt
     1681 caaaagatac cgagtggcag aaagaatttg agtataaatt tccttttgaa gaaacggaag
     1741 accagcttcg ttgcgtaaaa gaaatcaaaa aagatatgga aaagcctatt tgcatggaca
     1801 gacttctttg cggcgatgtc gggttcggaa aaaccgaagt ggcaatgaga gccgcattta
     1861 aagcagttat ggattcaaag caagtggcga tgttggtacc tacaaccata ttggcgcagc
     1921 agcattatac aaatttagtt gaaagattca gaggatatcc cattaaaatt gaaatgctga
     1981 gccgttttcg aacacattac caacaaaaca aaataatcaa cgatttaaat aagggtttga
     2041 ttgatattgt aataggaaca cataaacttc tttccaaaga gcttaaattt aaggatttag
     2101 gacttttgat aatagatgag gagcaaagat tcggtgtcag acataaggaa ttgataaaac
     2161 agcttaaatc aaatattgat gttctgacgc tgtcggcaac accaataccc cgaacacttc
     2221 atatgtctat gattggtgta agggacatga gcatcatatc tgaaccgccc ggagacagac
     2281 ttcctataca aacttatgtt attgaatata acgaaggatt gataaaggat gcaattgaaa
     2341 aggaaatttc aagaaagggt caggtttatt acgtacacaa cagagtggca gacatagacc
     2401 aaacagcagc aaagttgaga aagctgcttc ctgaggcacg aatagcagtg gctcacggcc
     2461 agatgagcga aagacatctt gaaaatataa tgcttgaatt tgtaaataag gaatatgata
     2521 ttttggtttg tacaaccata attgaaaccg gaatggacat acccaatgta aatacattga
     2581 ttattgataa tgccgattat ttgggacttt cacaactgta tcagcttcgg ggaaggatag
     2641 gcaggtcaaa caaagttgct tttgcatacc ttacctacga gaaggacaaa atgctgtctg
     2701 aagttgccga caaaagactt aaggcaataa aggaatttac cgagtttggc tcaggattta
     2761 aaatagctat gcgcgacttg gaaataagag gcagcggaaa tatattgggt ccggagcagc
     2821 atggtcatat gctggcaata ggatatgatt tatatgttaa gttcttagac cgggcagtta
     2881 aagaaattca aggaaaacaa gttgaggaag atattgaaac gtcggttgat ttaaatgttg
     2941 acggttatat tccttcatca tttattgaaa atgaagagca aaaaatagaa atttacaaaa
     3001 aaatagcagc agcagagaat aaagatgata tatttgatat aactgaagaa attattgaca
     3061 ggtttggcaa tattcctgtt caagtagata atcttttaaa aatatcatac attaaatcat
     3121 tgagcaaaag aattaaaata aaatcaatag ctcaggcagg caataccgta aacatagaaa
     3181 tgacgaaaga ttacctgagt caagatatca ttgagttttt aataaagaat tattatgaaa
     3241 aaataagctt tgttggaact aacgaaccga ttattaaata taaactggat tctcttgagc
     3301 aaatgaaaat tcttagggaa ctggaagagt ttttagaaac actaaataat tttaaagcag
     3361 gaaaaactgc tgtggaggaa aaacatgaac aaatttaaaa aatcattatt tttaattact
     3421 ttattatgca tagctttagt atttacagct tgctcaaact caggcggcaa agatgtcgat
     3481 aaagagggtg taatggcaac tgtaaacggc aagccgatta tgcagaaaga ttatgatgat
     3541 gcattggcat attataagga ttatgttgag tatcaattag gcgaagatgc atgggaaaca
     3601 gaaacaccaa ggggtactta taaagaaatt tatgaagaat acgtatggga tgaaatgact
     3661 aaatcattgc ttctgcttga tgctgccgaa aaagaaggaa taaaggcaag tgaagaagaa
     3721 gtacagcatt cattggaaaa ctttaaaatt aatttcgaaa acgatgaaga atataaaagt
     3781 tttatggagc aaagaggtat aacggaagat tttattattc aggagttaac caaagaaata
     3841 ttaattaata attatataac
//
LOCUS       DAOSOW010000165         3835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_23868_length_3835_cov_3.451323, whole genome shotgun sequence.
ACCESSION   DAOSOW010000165 DAOSOW010000000
VERSION     DAOSOW010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23868_length_3835_cov_3.451323"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PLD67_05190"
     CDS             <1..520
                     /locus_tag="PLD67_05190"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOT21948.1"
                     /translation="IYREMSLKEVAQAIAETVRDSAGILLIICGAAAFSWLVTMLGLT
                     ETFSNALTSLTDNKYVMLLILNIAFLIIGMFMEALAVMTITIPFLIPLMGSFGIDPVH
                     LGIVLILNLMIGLSTPPVGTSLFVCAKVADISIERMYKAILPFLIPLIFVLFLITYVP
                     SLVTWLPNMMLK"
     gene            733..1950
                     /gene="arcA"
                     /locus_tag="PLD67_05195"
     CDS             733..1950
                     /gene="arcA"
                     /locus_tag="PLD67_05195"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HOT21949.1"
                     /translation="MNKSINVYSEIGKLKKVLLHRPGRELENLMPEYLDRLLFDDIPY
                     LKIAQQEHDAFANIFRENGAEVVYLEDLVVESLINDEVNSKFIDEYIEEAGIKEDRET
                     GILKEYFLSFSDKKDMVLKMMEGIRKSEIKDYRGPGLADFLSNQYPFIIDPMPNLYFT
                     RDPFAALGSGVSIHKMMTATRNRETLFGKYIFNYHPTYKDTKLFYDRTEDYSLEGGDI
                     LVLNKETIAVGISQRTNPNAIEKFANIILTEESSFKKVLAIDIPKTRAFMHLDTVFTM
                     VDYDKFTIHPNIKSDIVVYVLKKIDGKMDITEERATLEQVLEKELNIDKIKLIKCGGD
                     SVIDASREQWNDGSNTLCISPGEVIVYSRNYVTNKILEEEGIKIHIMPSSELSRGRGG
                     PRCMSMPLIRENV"
     gene            2033..3028
                     /gene="argF"
                     /locus_tag="PLD67_05200"
     CDS             2033..3028
                     /gene="argF"
                     /locus_tag="PLD67_05200"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOT21950.1"
                     /translation="MAVNLKGRSFLTLMDFTPEEIRYLLDLSADLKRKKRSGSTDYLL
                     KGKNIVLLFEKTSTRTRCAFEVAALDEGAHVTFLDSGSSQMGKKESLEDTAKVLGRFF
                     DGIEYRGYEQKVVEDLAKFAGVPVWNGLTDVDHPTQILADMLTIEEHVAKPLNKVKVV
                     FAGDIRNNMSYAWMYGCAKMGMHFVAFGPKVLADQLDKEILKKVNEVAEYTGAKIEIS
                     DSIDSVKGADVIYTDVWASMGEEALIPDRVKLLTPYKVTMDLLKATENEDVIFMHCLP
                     AFHDFETKLAKEQKELGYDIREVTDEVFRSRHSVVFDEAENRLHTIKAVIVATIG"
     gene            complement(3054..>3835)
                     /locus_tag="PLD67_05205"
     CDS             complement(3054..>3835)
                     /locus_tag="PLD67_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HOT21951.1"
                     /translation="KAHMIIEKANIKEFKNLIQNIVTDFDNVELDESIKKPKVGIVGE
                     ILVKFHPNANNDVFSLLEEEGAEVVVPDLLDFFSYTAYNGVIKYDELLIGSKATKAIN
                     NIAVKIIEFYKRPSIDAFKNSKRFLTPVGIKELANKAKNLLSLANQCGEGWFLTAEMI
                     ELLESGINNILCLQPFACLPNHITGKGMIKKLKEAYPWSNIVPIDYDSGMSEVNQINR
                     IKLMLTNAVRNMKQELDTALLNSIEENAPDFNLQNMKEIEI"
BASE COUNT         1289 a          595 c          743 g         1208 t
ORIGIN      
        1 aatttatcgt gagatgagcc tcaaggaagt tgctcaagca atagcagaga cagtgcgtga
       61 ttcagcaggt atattactaa ttatatgcgg cgctgctgca ttttcatggc tggtcaccat
      121 gcttggtctc accgagacat tttctaatgc cttgacatct cttacggaca acaaatatgt
      181 aatgctctta atattaaaca ttgccttttt gataattggg atgttcatgg aggcattggc
      241 tgtcatgaca ataactatac catttttgat tccgcttatg ggcagttttg gaattgaccc
      301 ggtacatctt gggatagtat tgattctcaa tcttatgata ggtctttcca caccgcctgt
      361 tgggacatct ttgtttgtat gtgccaaggt tgcagatata tctattgaaa gaatgtataa
      421 ggcaattctt cctttcctta ttccccttat atttgtgctg tttctgatta catatgtacc
      481 gagccttgta acatggctac caaacatgat gcttaaataa caaaatactt acctggccat
      541 taaattttat ttatatgaaa cagttgtatt tcaactgttt tttatttacg ataaattaag
      601 gcggcaaccc ctgatgattt gtttaacaag ccttaattaa aagtgcataa atataatgct
      661 actttataaa tatgttaaat aagcttgaca aacaatggaa agattattag tatatatgta
      721 ggtggtgata atgtgaataa gtcgataaat gtatattccg aaatcgggaa attaaaaaaa
      781 gttttattgc acagacctgg aagagaatta gaaaatttaa tgccggaata tcttgacaga
      841 ttgctttttg acgatattcc gtatttaaaa attgcacagc aggaacatga tgcttttgca
      901 aacattttta gagaaaatgg agcagaagtt gtttatttgg aagatttggt cgttgagtct
      961 cttattaatg atgaagttaa ttcaaagttt attgatgagt atattgagga agcaggtata
     1021 aaggaagata gagaaaccgg tatcttgaaa gaatattttc tgtctttttc cgataagaag
     1081 gatatggtcc taaaaatgat ggaagggata cgaaagtctg aaataaaaga ttacaggggt
     1141 cccggtttag ctgacttttt aagcaatcaa tacccattta taattgaccc tatgccaaat
     1201 ttatatttta caagagatcc gtttgctgca ctgggcagcg gtgtatctat tcacaaaatg
     1261 atgactgcaa caagaaacag ggaaacttta ttcgggaagt atatatttaa ctaccatcct
     1321 acatataagg atacaaaatt attttatgat agaactgagg attattctct tgaaggcgga
     1381 gatatattag tgctgaataa agaaactata gcagtgggta tttcacaaag aactaatcca
     1441 aatgcaattg aaaagtttgc aaacataata ttgacagaag agagttcctt taaaaaggta
     1501 ctggcaatag acatacctaa aaccagagct tttatgcatt tggacacggt atttaccatg
     1561 gtcgattatg acaaattcac aatacatcca aatattaagt ctgatatagt tgtttatgtt
     1621 cttaaaaaaa ttgatggaaa aatggatatt acagaggaaa gagccaccct tgaacaggtg
     1681 ttggaaaaag aactaaatat tgataagatt aaattaatta agtgcggcgg cgacagtgtt
     1741 attgatgcct ccagggagca atggaacgac ggctccaata cattatgtat atcaccgggt
     1801 gaagtaattg tttattcaag aaattacgtt acgaataaaa tacttgagga agaaggaata
     1861 aagattcata taatgccaag ctctgagtta tcaaggggca ggggcggacc aagatgtatg
     1921 agcatgccct taatcaggga aaacgtataa agttgtaaaa gaattgaaat atttaaaatg
     1981 ttttgatata ttaagcatac aaaggatata aaattaataa tggaggatgt aaatggcagt
     2041 aaatttaaag ggaagaagtt ttttgacatt aatggatttt acaccggaag aaatcagata
     2101 cttacttgac ttatcagcgg atttaaaacg aaaaaagagg tcaggctcta ccgattattt
     2161 attgaaaggt aaaaacattg ttttattgtt tgaaaaaaca tctacaagaa ccagatgtgc
     2221 ttttgaagtt gctgcattag atgaaggcgc acacgtaact ttcttggatt caggaagctc
     2281 acagatgggt aaaaaagagt ctttggaaga taccgcaaag gtactcggga gattcttcga
     2341 cggtattgaa tacagaggat acgagcaaaa ggttgtagaa gatttggcta aatttgcagg
     2401 agttccagta tggaacggct taaccgacgt ggatcacccc acacaaatac tggcggatat
     2461 gcttacaata gaagaacatg tggcaaaacc tttaaataag gtaaaagtag tatttgcagg
     2521 agatataaga aacaatatgt catatgcatg gatgtacggc tgtgcaaaga tgggaatgca
     2581 ttttgtagca ttcggaccga aggtacttgc tgaccaatta gacaaagaaa tattaaaaaa
     2641 ggttaatgaa gttgctgaat ataccggagc aaaaatagaa atatcagaca gtatcgacag
     2701 cgttaaagga gctgatgtaa tatatacaga cgtttgggcc tccatgggag aagaggctct
     2761 gattcccgat agagtaaaat tactcacacc atacaaagta accatggacc ttttaaaagc
     2821 aacagaaaat gaagatgtaa tattcatgca ttgccttcct gcatttcatg actttgaaac
     2881 taaattggca aaagagcaaa aggaattagg ttatgatata agggaagtta ctgatgaagt
     2941 tttcaggagc agacattctg ttgttttcga tgaagcagaa aacagattac atacaattaa
     3001 agcggttatt gtagcaacaa tcggataata ataaagcacc ttttagaggt gctttatatt
     3061 tcgatttctt tcatattttg taaattaaaa tcaggtgcat tttcttcaat agagtttaaa
     3121 agagctgtat cgagctcctg tttcatattt cttacagcat tggtaagcat cagtttaatt
     3181 ctgtttattt gatttacttc gctcattcct gaatcgtaat ctatgggtac tatgttagac
     3241 cagggataag cttcctttag ctttttaatc attcccttgc ccgtaatgtg attcggcaga
     3301 catgcaaatg gctgaagaca caagatgttg ttaataccgc tctctaataa ctcaatcatt
     3361 tctgccgtta aaaaccaccc ttctccgcat tgattggcta aggacaataa atttttagcc
     3421 ttatttgcca attccttaat accgacagga gttaaaaacc ttttagaatt tttgaatgca
     3481 tcaatactcg gtcttttata aaactctata atttttactg caatattgtt tattgccttt
     3541 gtcgccttgc tgccaatcag cagttcatca tattttataa ctccgttgta agcggtgtag
     3601 gaaaaaaaat ccagaagatc cggtactaca acttcagcac cttcttcttc caatagtgaa
     3661 aacacatcat tgtttgcatt cggatgaaat tttacgagta tttcgcctac aattccgact
     3721 tttggctttt ttatgctttc atctaattct acattgtcaa aatccgtaac tatattttgt
     3781 atcagatttt taaattcttt aatatttgct ttttctataa tcatgtgagc ttttt
//
LOCUS       DAOSOW010000166         3809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_24095_length_3809_cov_3.155567, whole genome shotgun sequence.
ACCESSION   DAOSOW010000166 DAOSOW010000000
VERSION     DAOSOW010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24095_length_3809_cov_3.155567"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLD67_05210"
     CDS             <1..361
                     /locus_tag="PLD67_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOT21952.1"
                     /translation="CIAVCPQGAIRNTWGGSNFFEKLAEYAYGAAKDKDIIFITFVHN
                     ITKDCDCNGTHMKLIADNVGILAGKDPVSLDTACLDLVQKTEGNKLFDKGRNTLKHAE
                     KIGLGEMNYELIKIKIP"
     gene            373..498
                     /locus_tag="PLD67_05215"
                     /pseudo
     CDS             373..498
                     /locus_tag="PLD67_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006776251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
     gene            560..1060
                     /locus_tag="PLD67_05220"
     CDS             560..1060
                     /locus_tag="PLD67_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOT21953.1"
                     /translation="MYNYNVPYPNQFMLCSTYGMDFSMWAYNTALNLIREALQSERND
                     ELKFNYLIENAPTQEEKDIITTIRDDERLNRQWYRDIYKFYTNEEVENNNGGEFTEPD
                     SFLDGIKSSIFESLFAMQRSRIIREGLPSRFIRDIVLRILTDEMKHATEYNYILSINS
                     LNSNLG"
     gene            1313..1633
                     /locus_tag="PLD67_05225"
     CDS             1313..1633
                     /locus_tag="PLD67_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT21954.1"
                     /translation="MAKEQLKTLTEPMYYILLTLSEPQHGYGIMQEVERRTEGRVKIG
                     AGTLYNLLSRFEDENIIVQISEENRRKTYTLTDKGLNILMDEYQRLKQLVSDGSNFLE
                     GRAR"
     gene            1634..3004
                     /locus_tag="PLD67_05230"
     CDS             1634..3004
                     /locus_tag="PLD67_05230"
                     /inference="COORDINATES: protein motif:HMM:NF022641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HOT21955.1"
                     /translation="MKNKTKRVFWGFFSLDYKAMGEYLGEMAEKGWMLEKVGRYIAKF
                     RAIEPKKIKFYVDVFKEAGPLTPEKTESSEEYRKLCQESGWTFITSQDYLQFFYAARD
                     SQPVPIQTDEEIEQKIVEHTLLKNELRGVLIFAILAIFILVRNIPLKYTNLLSFTGVT
                     STFLFPVLCILTAIPAGYSIIWMIKARRNIKNGISITKPTWKSARRRTMLFYGPTWII
                     ILFYSMSFIIDSFFKPDIMAFVFLGPGIGTIFGLTIRYLVKNNKIAKDAVLNYFVIGM
                     IIVIIFVQIAGRLIFDWSEDIYMTDSIPDGYPIITLEEITKKTSEGSVRREFKPGMSP
                     IVPKHHTYYEYENLGDSARSINIKYYKAINSYFAEIIFNGITEKLEKGMKWRGMTIFT
                     KNIITDDEMKNLWGVDNLALTEERDELIIQKGNIVLHVDNYSKVMDFNDKETRKLIID
                     RFFPGS"
     gene            3355..>3809
                     /locus_tag="PLD67_05235"
     CDS             3355..>3809
                     /locus_tag="PLD67_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HOT21956.1"
                     /translation="MREIDASTYIMAAIIAHFAGLLTCGFFVKSQIAAKKMIVTGTIL
                     CLVVTIPFFFTPSALWMGSLVIGGFINGCAVAAWGYFLRAFTPKNERIKTCADVLIYS
                     NIIMIVLNVIAMNRAPLIGLILSMFCLVIGAAFILMLPAESTYENKILN"
BASE COUNT         1345 a          522 c          747 g         1195 t
ORIGIN      
        1 ctgtattgcg gtttgcccgc aaggtgccat taggaatact tggggcggtt caaacttctt
       61 tgagaaactt gcggagtatg cctatggagc agcaaaggac aaagatatta tattcattac
      121 atttgttcat aatatcacta aggattgcga ctgcaacgga acacatatga aactgattgc
      181 agataatgtc ggtattcttg caggtaaaga tcccgtatcc ttagataccg catgtttaga
      241 tttagtacag aaaacagagg gcaataagct ttttgataaa ggcaggaata ctttaaagca
      301 tgcggaaaaa atcggcctgg gtgaaatgaa ttatgagctt attaaaataa agatacctta
      361 atagatgtgc atattgtggt tgagcgcctt gttcccaagc tggccggttt aactttctgt
      421 aaaaagggac aaacaagtga attggcaaca aaacgctatc atgaaatcct tagaaatctc
      481 gataaagaga tagcataaat acaccgattg gtagggtaaa catatattat aattataata
      541 tataatcaga ggtgataact tgtataatta taatgttcct tatccaaatc aatttatgct
      601 atgttctact tatggaatgg attttagcat gtgggcatat aacactgctc taaacctaat
      661 aagagaggca ttgcaaagcg aaagaaatga tgaactaaaa tttaattatt tgattgaaaa
      721 tgcccctact caagaagaaa aagatataat aacaacaatt agagatgatg agagattaaa
      781 ccgacaatgg tacagggata tatacaaatt ttataccaat gaggaagttg aaaataataa
      841 tggtggagag tttacagaac ctgattcatt tttagatggt ataaaaagct ctatattcga
      901 atcgcttttt gcaatgcaaa gatctcgtat cataagagaa gggttaccga gcagattcat
      961 tcgagatata gtacttagaa tacttacaga cgaaatgaaa catgcaactg aatacaatta
     1021 cattcttagc ataaacagtt tgaactctaa tctcggttaa ccactgatta ttacatcatt
     1081 agtcctgata aatcagggct tttttctttg tgcatcattt tcaattatta agtattataa
     1141 aggttttatt ttattgaagt ttatggaaaa taacaaataa atttttaatc cttcaaaact
     1201 tattaaagat atatttttat attgtaaata gtgattgaca ttaagacaaa aaaattatat
     1261 tatatcaata atggatatat cgactatcga tatattgttt ggagatgata ttatggcaaa
     1321 agaacaatta aaaacgttaa ccgagccaat gtattacatc cttttaaccc tatcagagcc
     1381 tcagcatggg tacggaatta tgcaggaggt agaaagacgt accgagggca gggtgaagat
     1441 aggggcaggt actttataca accttttatc cagatttgaa gatgaaaata taattgtgca
     1501 gatttcagaa gaaaacagaa ggaaaacata tactttgacg gataagggat taaatatttt
     1561 aatggatgaa tatcaacggt taaagcaatt ggtttcagat ggcagtaatt tcttggaagg
     1621 gagagcgcgg taaatgaaaa ataaaactaa acgtgtattt tggggatttt tctcgcttga
     1681 ttataaagct atgggcgaat atttagggga aatggctgaa aagggttgga tgcttgaaaa
     1741 ggttggaaga tatatagcaa agtttagagc catagagcct aagaagatta aattctatgt
     1801 agatgtattt aaagaagctg gaccccttac tccggaaaaa actgaaagct cggaggaata
     1861 taggaagcta tgtcaagagt caggatggac atttataaca tctcaagact atttacagtt
     1921 tttctatgct gccagagaca gccaaccggt tcctattcag acagatgaag aaatagagca
     1981 aaaaattgtt gagcacactc tgttgaaaaa tgaactgcgt ggagtactta tattcgcaat
     2041 attggctatc tttatattag tcaggaatat acccctaaaa tatactaacc tcttaagctt
     2101 tacaggagtt acaagcacat ttttattccc tgtactatgc atacttactg caattcctgc
     2161 cggatacagc ataatctgga tgataaaagc aagaagaaat attaaaaatg gcatatccat
     2221 aacaaaacct acctggaaaa gtgcgcgaag gcggacaatg ttattttatg gtccaacatg
     2281 gattattata ttgttttatt ctatgtcttt tattatagat tcatttttta agcctgatat
     2341 tatggcattt gtatttttag gtcccggtat tggtaccata tttggtttaa caatcaggta
     2401 tctggtaaag aataataaaa ttgcaaagga cgctgttctc aattattttg taattggaat
     2461 gattatagta ataatttttg ttcaaatagc aggcagattg atttttgact ggtctgaaga
     2521 tatttatatg acagattcaa tacctgatgg ttatccaata attacattag aagaaataac
     2581 aaagaaaaca agtgaaggaa gtgtaagaag ggagtttaaa ccgggtatga gtcccatcgt
     2641 acctaagcac catacttatt atgaatatga gaatttagga gatagtgcga gaagtattaa
     2701 tattaaatat tataaggcaa taaattcata ttttgccgag attatattta atggaataac
     2761 agaaaaattg gaaaaaggaa tgaagtggag agggatgact atatttacaa aaaatattat
     2821 cacagatgat gagatgaaga atttgtgggg tgtggataat ttggcactga cagaggagag
     2881 ggatgagctt ataatccaaa aaggaaacat agtgttgcat gtagataact attcgaaggt
     2941 tatggatttt aatgacaaag aaacaagaaa gttgattatc gacaggtttt ttccgggttc
     3001 ttaactctat attataaagt tatatatatt gataaatata atctctaata ctttgtacca
     3061 ttccctcaag ataattttga gggaattttt gtattggcgt gaagagtttg gtgtagtaaa
     3121 cagtttcgca aattgtattg aacaaaaaag attcttatgc tacaattaga aaagtaatta
     3181 taaaatactt gcataaatat aaataaaaag gtataatcca tgcacgaaaa tacacatagc
     3241 aagagatgga aactaattaa tacacgtaga ctttctattg caggattctc gtttctattt
     3301 gcttatattt tatctttcca atttgaagga caggtgctgt atagcttatt gaatatgcga
     3361 gaaatagatg cctccactta tataatggct gcaattatag cacattttgc gggtctttta
     3421 acttgcggtt tttttgttaa atctcagatt gctgcaaaaa aaatgatagt gactggtacg
     3481 attttatgct tagttgtcac aatacctttc ttttttactc catcggcctt atggatggga
     3541 agcttagtta ttggaggatt tataaacggt tgtgcagtgg cagcatgggg atattttctt
     3601 agggcattta ctcccaaaaa tgagcgtatt aaaacctgtg ctgacgtgct gatttattcc
     3661 aatataataa tgattgtgct caatgtgatt gccatgaacc gagcaccctt gataggatta
     3721 attctttcca tgttttgtct tgtaattggt gcagctttta tattgatgct gccggcagag
     3781 tcgacgtatg aaaacaaaat attgaataa
//
LOCUS       DAOSOW010000167         3807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_24109_length_3807_cov_3.500533, whole genome shotgun sequence.
ACCESSION   DAOSOW010000167 DAOSOW010000000
VERSION     DAOSOW010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24109_length_3807_cov_3.500533"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..306
                     /locus_tag="PLD67_05240"
     CDS             82..306
                     /locus_tag="PLD67_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOT21957.1"
                     /translation="MKKIKIYTGSSCSNCTAAKEYIKEKGYEYEEKNTSADSEAKKEL
                     IALGYMGVPVIMIDDEVVVGFNKSKLDEIL"
     gene            complement(518..700)
                     /locus_tag="PLD67_05245"
     CDS             complement(518..700)
                     /locus_tag="PLD67_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003371961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOT21958.1"
                     /translation="MITKDMTIREVIMKNQEAAEILMTFGMGCVGCPSAQMETLEEAA
                     AVHGMNLDDLLAALNK"
     gene            938..1843
                     /locus_tag="PLD67_05250"
     CDS             938..1843
                     /locus_tag="PLD67_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21959.1"
                     /translation="MHNKYNEKISDMKKCIQKKNKLNLLLKQTEQDIIKEKLLLNKLS
                     GELEKEYQDALKLKSDNIISLFYEILGTEEDKHNKEKQKLLKARLKYEQCKNNMNYLV
                     NETKKIVDCIEKLNGCDAEYEELINKKIETINIEDNETGQALKKLIKRKENMNANIIE
                     ICEAICYGEKALEAIEMTIKELEAAENFENSDGSNNIENARGYAEQTHRMLGRFKKEL
                     SDITIVTGTEIAVGTFENFADCFFDNLIFDWVVQSGIRKSLDTVKNTKNQLDKAMSKL
                     YEEKVTEEFMIKQIEEQINQIIEKV"
     gene            2100..3083
                     /locus_tag="PLD67_05255"
     CDS             2100..3083
                     /locus_tag="PLD67_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21960.1"
                     /translation="MSEYVLEIRDLSICFYSGRKEIPAVENLSFNLSRGETLGIVGES
                     GSGKTVTSLSIMKLIPNPPGKITGGEMFFGSLDLLKVDEDTIRQIRGNNISMIFQEPM
                     TSLNPVYTIGEQLSEVFCTHQKLSKKEAMKKSLDMLKQVKIPLAEKRMKQYPHELSGG
                     MRQRVMIAMALACMPEILIADEPTTALDVTIQSQILHLIREMKEKMNTAVILITHNMG
                     VVAEMADNVMVMYCGRALEYADVRTLFKEPKHPYTIGLLNSIPRIKGGRKKLYAIKGS
                     VPVPGEITAGCRFNSRCEYVFEKCLVEEPPIFEVGKSKVRCWKYEGKGVTV"
     gene            3080..>3807
                     /locus_tag="PLD67_05260"
     CDS             3080..>3807
                     /locus_tag="PLD67_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007202729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HOT21961.1"
                     /translation="MSGNILEVKNLKVHFPVKGELIPKNVGYVKAVDGISFNIGYAET
                     LGLVGESGCGKSTTGRAVLNMLKVDSGQVFFEGKDILKSSRKELKKIRRDMQLIFQDP
                     FGSLNPRMTIGSALEEVLYIHHIRDKNERQKRVIELIEMVGLNKNYLSRYPHEFSGGQ
                     RQRAVIARALAVKPKFIVCDEPVSALDVSIQSQIINLLEELQKNFKLTYLFIAHDLSV
                     VKHISNRIAVMYLGRIVEIAESDE"
BASE COUNT         1406 a          518 c          781 g         1102 t
ORIGIN      
        1 atatttatgg tattaaatga agtaatatgg taaaataaat taaaaattag gcgatatgca
       61 tactaacaac aggaggataa aatgaaaaaa ataaaaattt ataccggctc atcatgttca
      121 aattgcactg ctgcaaagga atacattaag gaaaaaggat atgaatatga ggaaaaaaat
      181 acttccgctg atagtgaagc aaaaaaagaa ttaattgcct tgggatatat gggggttccg
      241 gtaattatga ttgatgatga agtagttgta ggattcaata aaagcaagtt ggatgaaata
      301 ttgtaagggt tgtttatgag ttttattgaa gagttacaga tagaacatga tgcagtttac
      361 attattaact aaatggctaa gatatttaca tattaatatt tttaataatg taaataatat
      421 aaataagcaa tataaacagc tgttttattg cttttaaata taaaaaccgg ctttatgccg
      481 gtttttgctg ggcagattga ctgcttgttg taaagtctta tttattcaag gcagctaata
      541 aatcatctaa attcattccg tgtactgctg cggcttcttc caatgtttcc atttgagcac
      601 ttgggcagcc tacacatccc attccaaatg tcattaatat ttcggcagct tcctggtttt
      661 tcataataac ttcacgaatt gtcatgtctt ttgtaatcat ttattgcctc cttttaattt
      721 ataattcatt atacagtaag ataagatatt attcaatatt ttaattaata aaaagttacc
      781 tgactctatt tgataatttt gtatcaagaa tagtttttaa taataaataa aaacgtattc
      841 ccgttttttc agactaaaaa ctgtttgaat cgccataaga tgtatgatat aatcaaaaaa
      901 gttaacagta acaatagcaa agggcgggag caagaatatg cataataaat acaatgaaaa
      961 aataagcgac atgaaaaaat gcatacaaaa aaagaataaa ttaaatttgc ttttaaaaca
     1021 aacggagcaa gatattatta aagaaaaact tcttctaaat aagctttcgg gcgaattgga
     1081 gaaggaatat caggatgctt taaagctaaa atcagataat attatttcgt tgttttatga
     1141 aatattgggg acggaagaag ataaacacaa caaggaaaag cagaagctat taaaagcaag
     1201 gttaaaatat gaacaatgta aaaacaacat gaattatcta gttaacgaaa ctaaaaagat
     1261 tgtagattgt atagaaaaat taaacggatg cgatgctgag tatgaagaat taataaacaa
     1321 aaagatagaa actattaata tagaggacaa tgaaaccggc caggctttaa aaaaactaat
     1381 aaaaagaaaa gaaaatatga atgccaatat aatcgaaatt tgcgaagcaa tttgttatgg
     1441 tgaaaaagca ctggaagcta tagaaatgac aataaaagag ttagaagctg ctgaaaactt
     1501 tgaaaattca gatggaagca acaatattga aaatgcaagg gggtatgcgg agcaaaccca
     1561 tagaatgtta gggagattca agaaagaatt atccgacata acaattgtga ctggaactga
     1621 aatagcagta ggtacctttg aaaattttgc agactgtttt tttgacaacc ttatttttga
     1681 ctgggttgtt cagtccggaa taagaaaatc attagataca gtaaaaaata ctaaaaatca
     1741 attagacaag gcaatgtcaa aactatatga agaaaaagta acagaagaat tcatgataaa
     1801 acaaatagaa gaacaaataa atcaaataat agaaaaagta taaaggtatt gacctattat
     1861 attatttagt gatacaattg caatactaaa ttaataatag cgttgtctaa atttacattt
     1921 ctaatctatc actttctaaa ttaatttaca ttaataattc ttattaaaaa tagttgattt
     1981 cacaatacag gaaaatgttc tcataaagat tgctgtttga tttgatcttt tatttatatt
     2041 cggagaagca tagtctcctg caggttatta tttttatttt gaagattagg tgattgacta
     2101 tgagcgagta tgtgttggaa ataagggatt tgagcatttg tttttacagc ggcagaaagg
     2161 agatacctgc cgtagagaac ctcagcttta atctttcaag gggagaaaca cttggtatag
     2221 taggagagtc cggctcaggc aagactgtaa cttctttatc cattatgaag cttataccaa
     2281 acccgcccgg gaaaataacc ggaggagaaa tgttttttgg cagcctggat ttgctgaaag
     2341 tagatgaaga tacaatcagg caaatacggg gtaacaatat atccatgata tttcaggagc
     2401 ccatgacttc tctgaacccg gtttatacaa ttggagagca attatcggaa gttttttgta
     2461 cacatcaaaa gttgtccaag aaagaagcaa tgaaaaaatc attggatatg ttaaaacagg
     2521 taaaaattcc attggcagaa aaaagaatga agcaatatcc ccatgagttg agcgggggta
     2581 tgcgccaaag ggttatgatt gcaatggcac ttgcctgtat gccggagata ctgattgccg
     2641 atgagcctac cacagccctg gatgtaacaa tacaatcaca aattttacat cttataagag
     2701 agatgaagga gaagatgaat acagccgtta ttttaattac tcacaatatg ggcgtagtag
     2761 ctgaaatggc tgataatgtt atggttatgt attgcggaag ggccttagag tatgcggatg
     2821 tcagaacatt attcaaagag cccaagcatc cttataccat aggtttattg aattccatac
     2881 caagaattaa aggcggaagg aaaaaactat atgccattaa gggctcggta cctgttcccg
     2941 gtgagattac ggcagggtgt cggtttaata gccgctgtga atatgtattt gagaaatgcc
     3001 tggttgaaga accacctatt ttcgaagttg gaaaaagcaa ggtcaggtgc tggaaatatg
     3061 aagggaaggg tgtgactgtg tgagtggaaa tatattagag gtaaagaatt tgaaggtcca
     3121 ctttccggtc aaaggagagc tcattcctaa aaatgtcggt tatgtaaagg cggtggacgg
     3181 tatcagcttt aatataggct atgccgagac tctcggatta gtaggtgaaa gcgggtgcgg
     3241 gaaatcaaca accggtcgtg cagtcttgaa tatgctgaag gtagattcgg gacaagtttt
     3301 ctttgaaggc aaggatatcc tgaagagcag caggaaagaa ttgaaaaaga taagaaggga
     3361 tatgcagctg atatttcagg acccctttgg ctcacttaat cccaggatga ccatcggaag
     3421 tgccttagaa gaggtcttgt atatacacca tattagggat aagaatgagc ggcaaaaaag
     3481 agtaattgag ctcattgaaa tggtaggctt aaataaaaat tatttatcga ggtatcctca
     3541 tgagttcagc ggcggccaaa ggcaaagggc tgtaattgca agagctttgg ctgtaaagcc
     3601 aaaatttatt gtttgtgatg agcctgtgtc tgcactggat gtttccattc aatctcaaat
     3661 tattaattta ttggaggagc ttcagaaaaa ctttaagctt acatatctgt ttattgctca
     3721 tgacttaagt gttgtcaaac atataagcaa ccgaattgca gtaatgtatt taggcaggat
     3781 tgtggagatt gcagaaagcg atgagtt
//
LOCUS       DAOSOW010000168         3797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_24202_length_3797_cov_3.503474, whole genome shotgun sequence.
ACCESSION   DAOSOW010000168 DAOSOW010000000
VERSION     DAOSOW010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24202_length_3797_cov_3.503474"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="PLD67_05265"
     CDS             <1..587
                     /locus_tag="PLD67_05265"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOT21962.1"
                     /translation="YIGDVAGHGVSAAMLTVFANQNIKPIKEGEIFEIISPGYVMKSL
                     YKSFNETHYKIESYLVMLYGIYNTKSRQLTYTSAGINVPPLIIKSTGEILEMKIEGFP
                     ICKLGNFYMPYFEDISIQLNVGDIVLFYTDGLVEYSNENGIMYSHNDLKDFLIKHYSM
                     KASELYSAIKENLFKKIGNKKIADDVTFLIMEIY"
     gene            777..2969
                     /gene="topA"
                     /locus_tag="PLD67_05270"
     CDS             777..2969
                     /gene="topA"
                     /locus_tag="PLD67_05270"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOT21963.1"
                     /translation="MKNLLIVESPAKAKTIRTILGSNYTVKASVGHVRDLPKSKLGID
                     IENNFEPSYITIRGKGDIIQELKKEAKKADNVFLATDPDREGEAISWHLAKILNLDEN
                     KEIRIEFHEITKDAIKNASKAPRKLNIDLVNAQQARRVLDRLVGYKISPLLWKKIKKG
                     LSAGRVQSVALKLVIDREKEIMNFKPEEYWTLELEIKKSRKKFIAKYVGYFENDKLIK
                     VKINNQDEINKILESIDKEKITVAKIEKSKSYSKPGPPFTTSSLQQEANRKLNFTSKK
                     TMMVAQQLYEGVKVKGEGNVGLVTYIRTDSFRLSDEAVENAKQFILSNFGEKYYKYRV
                     YKKSKKNESKVQDAHEAIRPTSLLRTPEKIKDSLTSDQYKLYSLIWKRAVACQMADAE
                     YDVTKVLFNNNDNIFESNGKVLVFDGFRAVYGYADEKDEKMFPEVKINEVLDISKVIP
                     EQHFTQPPARYTEASLIKDLEEKGIGRPSTYAPTITTITSRDYVAKEKGYLIPTDMGF
                     LVTEMMEKYFPKIVNEKFTADLENHLDDVASGNINWRNVISNFYESFKKELDVAENEM
                     KEIEIKDEVSDVKCDNCGAFMVIKSGRFGKFLACPNYPECKNTKPPNGKEPPEEVNET
                     CEKCGSKMLKRKGKFGEFLACSNYPKCKNTKPIIKTIDAPCPRCGKQIAIKFSKAGRR
                     FYGCTGYPDCKYISWYEPTDKKCPECGEMMVLKNNKQTCINKKCKKSE"
     gene            3150..>3797
                     /gene="rpsB"
                     /locus_tag="PLD67_05275"
     CDS             3150..>3797
                     /gene="rpsB"
                     /locus_tag="PLD67_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOT21964.1"
                     /translation="MAIISMKKLLEAGVHFGHQTRRWNPKMAEYIFTERNGIYIIDLQ
                     KTSDKVDEAYNFIKEVVSNGDEILFVGTKKQAQESTKSEAIRCGMHYVSQSWLGGMLT
                     NYSTIRKRIDRLNELKTMEEDGTFDLLTKKEVFKLRNEAERLEKFLGGIKNMDILPGA
                     LFVVDQRKERIAVQEAAILGIPVVAIVDTNCDPDEVDYVIPGNDDAIRAVKLLTAT"
BASE COUNT         1491 a          555 c          743 g         1008 t
ORIGIN      
        1 tatatatagg agatgttgca ggacacggtg tatctgccgc catgcttacg gtttttgcca
       61 atcaaaatat taagcctata aaggaaggcg aaatatttga aataatttct cccggttacg
      121 ttatgaaaag tctttacaag tctttcaatg aaacacatta taaaattgaa tcatatcttg
      181 taatgcttta cggcatttac aatactaaaa gccgacaact tacatatact tcagcaggaa
      241 tcaatgttcc tcccttaata attaaaagta ccggtgaaat attggaaatg aaaatagaag
      301 ggtttcccat atgtaaactt gggaactttt acatgccata ttttgaagat ataagtattc
      361 aattaaatgt cggtgatata gttttattct atactgacgg tttggtagaa tacagcaatg
      421 aaaatggtat tatgtacagc cataatgatt tgaaagattt tctgattaaa cattattcaa
      481 tgaaagcatc ggaattatat tcggccataa aggaaaattt attcaaaaaa ataggtaata
      541 aaaaaattgc cgatgatgta acatttttaa ttatggaaat atattgagca acaatacatg
      601 ttatcccgag cgtaagcaag gatctcatga gtcgttgtat aataaataat cagtctagcc
      661 atcatatatg atggctattt taatatgtta acaatcataa actattgaca aataaaaaat
      721 tgtattataa ggtgaataag cacgggatat aaatttgcct aatcaatgga ggaaacatga
      781 agaatttatt aattgtagag tctcccgcca aagctaagac tatacgtaca atattaggaa
      841 gcaattacac tgttaaagct tcagtaggac atgtgcgaga tttacccaag agcaagctgg
      901 gtatagatat agaaaataat tttgagccaa gttatataac aataagggga aaaggggata
      961 taattcaaga actgaaaaaa gaagcaaaaa aagctgacaa tgtgtttctt gcaacagacc
     1021 ctgacaggga aggtgaagct atatcttggc atttggctaa aatattaaac ttggatgaaa
     1081 ataaggaaat aagaatcgaa tttcatgaaa taacaaagga tgcaattaaa aatgcaagca
     1141 aagctccacg aaagctcaat atagatttgg ttaatgctca acaagccaga cgtgttctag
     1201 acagattggt aggttataag ataagccctc tgttatggaa aaaaataaag aaaggtttaa
     1261 gtgcaggcag agttcaatct gttgcgttaa aattggttat agacagagag aaggaaataa
     1321 tgaatttcaa gcctgaagaa tattggacat tggagcttga aataaaaaaa tcaaggaaga
     1381 aatttattgc caaatatgta ggttattttg aaaatgataa attaatcaaa gtcaaaatca
     1441 ataatcagga tgaaataaat aaaatactcg aaagtatcga taaagaaaaa attaccgtag
     1501 caaaaattga aaaatccaag agctactcaa aacccggccc tccgtttaca acaagctcgc
     1561 ttcaacagga agcaaacaga aagcttaatt ttacatccaa aaaaacaatg atggttgccc
     1621 aacagcttta tgaaggagta aaagtcaaag gtgaagggaa tgtgggcttg gttacttata
     1681 tacgaaccga ctcattcaga ttgtcagatg aagccgttga aaatgcaaag caatttattt
     1741 tatcaaattt tggagaaaag tattataaat acagagtata taaaaagtca aagaaaaacg
     1801 aaagcaaagt gcaggatgca cacgaagcta taagacccac ttctttgctt cgaacaccgg
     1861 aaaaaattaa agacagccta acttcagacc aatataagct gtacagtctg atatggaaaa
     1921 gagctgttgc ctgtcagatg gcagatgcag aatatgatgt tactaaagtt ttatttaata
     1981 acaatgacaa catttttgaa agcaacggga aggttcttgt ttttgacggt tttagagctg
     2041 tatacggata tgctgatgaa aaagacgaaa aaatgtttcc cgaagtaaaa attaatgaag
     2101 tattagatat ctcaaaagta atacctgagc agcattttac ccaacctccc gctcgttata
     2161 ccgaagcaag tctcataaag gatttggagg aaaagggaat agggcgtcca agcacatatg
     2221 ctcccactat tacaactata acaagcaggg attatgtggc taaggaaaaa ggatatttga
     2281 ttccaacaga catgggtttt ttagtaaccg aaatgatgga gaaatacttc ccgaaaattg
     2341 tgaatgaaaa atttaccgca gatttagaaa accacttaga tgatgttgca agcggaaata
     2401 ttaattggcg caacgtaatt tcaaattttt acgaaagctt taaaaaagag cttgatgtag
     2461 cggaaaatga aatgaaagaa attgaaatca aagatgaagt aagtgatgtc aaatgtgaca
     2521 actgcggcgc attcatggtt attaaaagcg gaagattcgg aaagtttctt gcatgtccca
     2581 attatcccga atgtaagaat acaaagccgc caaacggcaa agaaccgcct gaggaagtaa
     2641 atgaaacatg cgaaaaatgc ggctctaaaa tgcttaagag gaaaggaaag ttcggagaat
     2701 ttttggcatg ctccaattat cccaaatgta aaaatacaaa accgattatt aaaactattg
     2761 atgctccctg tccgcgatgc ggcaagcaaa ttgcaatcaa gttttcaaaa gcaggacgga
     2821 gattttacgg atgcacaggt tatcctgatt gtaaatatat ttcatggtat gagccgaccg
     2881 acaaaaaatg tcccgaatgc ggcgaaatga tggttttaaa aaacaataag caaacatgca
     2941 taaataaaaa atgtaaaaaa agcgaataat attattgaaa aatagcttat aatatgcaca
     3001 tttttgtaat taaatattgc caaataaaat tggctatgat atactaatca taattacaca
     3061 cagattttga tcatgttaga gatgtgccgg aaacggtctt tttcatgata tgacatttct
     3121 ggaggtaaaa caaaaggagg aaaaaaataa tggcaattat cagtatgaaa aaattgctcg
     3181 aggccggggt tcattttgga catcaaacaa gaagatggaa tcctaaaatg gcagagtata
     3241 ttttcacgga aagaaatgga atctatatta tcgatttaca gaaaacaagc gataaagtag
     3301 atgaagctta caactttata aaggaagttg tatcaaacgg cgacgaaata ctatttgtcg
     3361 gaacaaaaaa acaagctcag gaatcaacaa agtctgaagc tataagatgc ggaatgcatt
     3421 atgtaagcca aagctggctt ggtggaatgc ttactaacta ctcaaccatc agaaaaagaa
     3481 ttgacaggct taatgaattg aaaacaatgg aagaggatgg tacattcgac cttttaacca
     3541 aaaaagaagt tttcaaatta agaaatgaag ccgaaagact tgaaaaattc ttaggcggaa
     3601 taaaaaatat ggatatactg ccgggtgctt tgtttgtagt tgaccagaga aaagaaagaa
     3661 tagctgttca ggaagcagca atcttaggaa ttcctgtagt agcaatagtt gacacaaatt
     3721 gtgaccctga tgaagtagat tatgttattc cgggcaatga tgatgctatc agagccgtaa
     3781 aactgttgac agcaact
//
LOCUS       DAOSOW010000169         3759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_24504_length_3759_cov_2.906048, whole genome shotgun sequence.
ACCESSION   DAOSOW010000169 DAOSOW010000000
VERSION     DAOSOW010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24504_length_3759_cov_2.906048"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLD67_05280"
     CDS             <1..455
                     /locus_tag="PLD67_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOT21965.1"
                     /translation="ISTIKQMENKKLLTEAESKIAISTYAEKIEEFFQSKIGKRIIDS
                     SAVYREAPFVLRKKANDISGSLNKDDLILVQGIIDCYFIEDGEAVIVDYKTDKIDETK
                     EIEFQINNLKSEYKDQIELYKEAVEKITSKNVKECYLYLFSIGKEVKI"
     gene            545..1195
                     /gene="deoC"
                     /locus_tag="PLD67_05285"
     CDS             545..1195
                     /gene="deoC"
                     /locus_tag="PLD67_05285"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012423789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOT21966.1"
                     /translation="MKYIELMKYVDHTLLKAYATWEEIRVLCDEAINYQTASVCIPPN
                     YVKRVHDTYQHKLNICTVVGFPLGYSTTEAKVAECVQALNDGADEIDMVINISDVKNK
                     DYKKVEDEIRTIKETVGEHILKVIVETCYLTNDEKIEMCHAVTKASADYIKTSTGFGT
                     GGATMEDVQLFKKYIGQNVRIKASGGIRTLNDMEAFINAGCSRIGTSSAIKLINNA"
     gene            1239..2423
                     /locus_tag="PLD67_05290"
     CDS             1239..2423
                     /locus_tag="PLD67_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT21967.1"
                     /translation="MKLWRKLAIVALVFIMIFAFSACANNQPEPPEEPETPETPETPE
                     TPEEPEKPAANVFELALITDVGTIDDKSFNQGAWEGVEAYAKEHNITYKYYQPAEQSD
                     TAYIDSIGLAVKAGAKVIVCPGFLFEVPIHTVQTMYPEVNFVILDGAPHAGDWVPDIA
                     DNTYSIFYAEEEAGFLAGYSIVKEGYTKLGFMGGMAVPAVVRFGYGFVQGAEYAAHEM
                     GLTDPVQIKYTYVGNFDATPENQAKAASWYNEGTEVIFACGGAVGNSVMKAAEAAGTK
                     VIGVDVDQSAESETVITSSMKNLSKSVYDALTAYYAGNFPGGTSVTLDATVEGVQLPM
                     DNSRFETFTQADYDAIYGKITAKEIEIMNDAEVVEDAGKPAEEVTAADIPVSKVQVEV
                     IQ"
     gene            2515..>3759
                     /locus_tag="PLD67_05295"
     CDS             2515..>3759
                     /locus_tag="PLD67_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21968.1"
                     /translation="MEYVIEMNNITKIFGNFKALDNVTFNLRKGEVHALLGENGAGKS
                     TLMSVLFGLYQAEKGSIKINNKTVKINNPNDASSLKIGMVHQHFKLVHNFTVLESIIL
                     GRETVNKGILKKDDARKKVMELSERYKLRIDPDAYISDITVGMQQRVEILKMLYSDSE
                     ILIFDEPTAVLTPQEIEELMKIMKELVKEGKSIVFISHKLNEIKEAADRCTVLRKGKY
                     IGTVDVKTTTKEQMSEMMVGRKISFNIEKDDIETGEAVLEVKNLTVKAKNSKKNMVNN
                     VSFKVKAGEILCIAGIDGNGQSELVYGITGLIPINEGQIFFKGKDITKYSVRKKSING
                     MSHIPEDRHKHGVILDFDLKQNAVLQTYFQPRFQKRGFIKFNAVSEYAKKLIKQYDIR
                     SPRDEDSIVRNMSGGNQQKLIVA"
BASE COUNT         1421 a          550 c          766 g         1022 t
ORIGIN      
        1 taataagcac aataaaacaa atggaaaata aaaagcttct tacagaagct gaatcaaaaa
       61 tagccattag tacatatgca gaaaaaatcg aagaattctt ccaaagcaaa ataggaaaaa
      121 gaataattga ctcttcagcg gtttacaggg aggcaccctt tgtattaaga aagaaagcca
      181 atgatatatc aggaagttta aacaaagatg atttaatact tgtacaaggt ataattgact
      241 gttatttcat tgaggatgga gaagcagtaa tagttgatta taaaacagat aaaatagacg
      301 aaacaaaaga aattgaattt caaatcaaca atcttaaaag tgaatataaa gaccaaattg
      361 aattatacaa ggaggcagta gaaaaaatca caagcaaaaa cgtaaaagaa tgctatttat
      421 acttattctc aatcggaaaa gaggttaaaa tataacattc ttcgcccctg cacctgttaa
      481 tcaatgtttg acgaacctat tattttatgc tagacttatt ttataaaaag tattggaggt
      541 atcaatgaaa tatattgaat tgatgaaata tgttgaccat actttattaa aagcatatgc
      601 aacatgggaa gaaattcgtg ttttgtgtga tgaagcaatt aattatcaaa cagcttcagt
      661 atgtattcct cccaattatg ttaaaagagt acatgataca tatcaacaca aattaaatat
      721 ttgcactgta gttggttttc ccttaggtta ttctactact gaagcaaagg ttgcagaatg
      781 tgtacaagct cttaatgacg gtgctgatga aatagatatg gttataaata tatctgatgt
      841 aaaaaacaaa gattataaga aagtagaaga tgaaatccgc acaattaaag aaacagtcgg
      901 ggaacacatt ttaaaggtaa ttgttgaaac atgttatttg accaatgacg aaaaaattga
      961 aatgtgccat gcagtgacta aggccagtgc ggattatatc aagacatcaa caggctttgg
     1021 tacaggtggc gcaactatgg aggatgtaca actttttaaa aaatatattg gacaaaatgt
     1081 tagaattaaa gcttccgggg gtataagaac attaaatgat atggaagctt ttattaacgc
     1141 aggatgttca agaataggaa ccagttctgc cattaaattg ataaataacg cttagcgttt
     1201 atttataaaa taataaaaaa aatggaggaa ttaatattat gaaattatgg agaaagttgg
     1261 ctattgttgc acttgtgttc ataatgattt ttgctttttc agcatgtgct aacaatcagc
     1321 cggaaccacc ggaagaaccg gaaacaccag aaacaccaga aacaccggaa acaccggaag
     1381 aaccggaaaa gccggcagct aatgtttttg aattagcgtt aatcactgac gttggaacaa
     1441 ttgatgacaa atcattcaac caaggtgcct gggaaggcgt agaagcttac gctaaagaac
     1501 ataatattac atacaaatat tatcaacctg ctgagcaatc agacactgct tatattgact
     1561 ctataggatt agcagtaaaa gcaggagcaa aggttatcgt atgtcctgga tttttgtttg
     1621 aagtgccgat tcatacagtt caaacaatgt atccggaagt aaactttgta attcttgatg
     1681 gtgcaccaca cgcaggcgat tgggttcctg acattgcaga caatacatat tcaattttct
     1741 atgctgaaga agaagcaggt ttcttagcag gatacagcat agttaaagaa ggctatacca
     1801 agttaggatt tatgggcggt atggctgttc ctgcggttgt tcgtttcgga tacggatttg
     1861 ttcaaggtgc tgaatatgca gctcatgaaa tgggattaac agaccctgtt caaattaaat
     1921 acacctatgt aggaaacttt gatgctaccc ctgaaaatca agcaaaagca gcttcttggt
     1981 acaatgaagg aacagaagta atatttgctt gcggcggtgc agtaggtaac tctgtcatga
     2041 aggcagcaga agcagcagga acaaaagtta taggtgttga cgttgaccaa tcagctgagt
     2101 cagaaacagt tataacttca tcaatgaaga acctttcaaa atccgtatat gatgctttaa
     2161 cagcatatta tgcaggaaac ttcccgggag gtacttcagt tacattggat gctactgttg
     2221 aaggtgttca attgccaatg gataactcaa ggtttgaaac tttcacacaa gctgattatg
     2281 atgcaattta cggaaaaata acagcaaaag aaatagaaat aatgaatgat gcagaagttg
     2341 tagaagatgc aggaaaacct gcagaagaag taactgcggc tgatattccg gtaagcaaag
     2401 tacaagtaga agtaattcaa tagttatatt aaagggacat tcctcttacc ctcaaagact
     2461 aaccccatag tagaggtgtg tcccctttcc atatcaggcg ggcggggtgg taagatggag
     2521 tacgtaattg aaatgaacaa tattaccaaa atattcggta attttaaagc tcttgataat
     2581 gtaaccttta atctcagaaa aggtgaagtc catgcgcttt tgggagaaaa tggagccggc
     2641 aaatctactt tgatgagtgt tttattcggt ttatatcaag cagaaaaagg cagtataaaa
     2701 attaataaca agactgttaa aattaataat cctaatgatg caagcagctt aaaaataggc
     2761 atggttcacc agcatttcaa attagttcat aattttacgg ttcttgaaag tattatttta
     2821 ggcagagaaa cagtaaataa gggtatttta aagaaagatg atgcaagaaa aaaggtaatg
     2881 gaactcagcg aacgttacaa gcttagaatc gacccggatg catatatttc ggatataacg
     2941 gttggaatgc agcaaagggt tgaaatactt aaaatgctgt attcagacag cgaaatatta
     3001 atttttgatg agcctacagc tgttttgacg cctcaggaaa ttgaagaatt aatgaaaata
     3061 atgaaagaat tggttaagga aggaaaatca attgttttta tatcccataa attaaatgag
     3121 ataaaagaag cagccgaccg ctgcacagtc ttacggaagg gcaagtatat aggaaccgta
     3181 gacgtaaaaa caacaaccaa agagcagatg tccgaaatga tggtgggacg taaaataagt
     3241 tttaacatag aaaaggatga tattgaaaca ggcgaagctg ttcttgaagt taaaaatttg
     3301 actgtaaaag caaagaattc aaagaaaaat atggttaata atgtttcttt taaagttaaa
     3361 gccggagaaa ttctttgcat tgcaggtatt gacggtaacg ggcaatccga gctggtatat
     3421 ggaataacag gactcatacc gattaatgaa ggacaaatat tctttaaggg aaaagatatt
     3481 actaagtatt cggtcagaaa aaaaagtatt aacggaatgt cgcatatacc tgaagacagg
     3541 cataagcacg gagtaattct ggattttgat ttgaaacaga atgctgtttt acagacttac
     3601 ttccagccgc gttttcaaaa aagaggattt atcaaattta atgctgtaag tgaatatgcc
     3661 aaaaaattaa taaaacaata cgatataaga agtccgagag atgaagattc aatagtgaga
     3721 aatatgtccg gaggaaatca acaaaagctt atagttgca
//
LOCUS       DAOSOW010000170         3748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_24597_length_3748_cov_3.727593, whole genome shotgun sequence.
ACCESSION   DAOSOW010000170 DAOSOW010000000
VERSION     DAOSOW010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24597_length_3748_cov_3.727593"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="PLD67_05300"
     CDS             complement(<1..786)
                     /locus_tag="PLD67_05300"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HOT21969.1"
                     /translation="MNKFEKISAMINNTPMLEISLKYQGLRRKVYAKAEYYNFTGSIK
                     DRIAYNILKYSYEAGLIKKGDIIAEASSGNTGIAFSAMGAFLENPVHIFMPDWMSEER
                     KKLLESFGATIHLVSKKDGGFKGSVESADKFGAENNAFLPHQFSNLLNAETHYKTTGA
                     EILRQLDAIGKKPDGFVAGVGTGGTIMGVKNALQEKYPNCKAFALEPIQSPAMSSGGM
                     VSGFHRIDGIGDGMIPEIVKLEQLDTVIRVDDGDAINMARQLAL"
     gene            959..2179
                     /locus_tag="PLD67_05305"
     CDS             959..2179
                     /locus_tag="PLD67_05305"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: protein motif:HMM:NF009814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOT21970.1"
                     /translation="MLIKMPKEVETALNILHVNGFEAYIVGGCVRDSIMGKVPSDWDV
                     ATSAKPEEILLCFSQYRTIETGIKHGTITVIINKMQIEITTYRIDGEYSDNRRPDNVL
                     FTDKIEFDLKRRDFTINALAYNKNGVIDLFGGLDDIKNKIVKCVGNSDERFNEDGLRI
                     LRALRFASVLNFDIDENTSCSIYGNKNLLNNISKEKINIELNKLLMGSNFQNILLEYR
                     DVIEVFLPEIRDLTKEKFEQRLKAMKKLNSLVLRLTVLLYEIDSPDKILMNLKYDNKT
                     VKTVKLLAENIDEKIYPDRVNIKRWLNKINHVNLKYLINIKKALFDYEYEELAKSEVI
                     MKEIIETNQCYSLKTLAINGQDLIDSGMPKGKNIGIVLNDVLNKVIEGKLENKKDILF
                     NYIYDRRKEIETPN"
     gene            2286..3692
                     /locus_tag="PLD67_05310"
     CDS             2286..3692
                     /locus_tag="PLD67_05310"
                     /inference="COORDINATES: protein motif:HMM:NF025774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HOT21971.1"
                     /translation="MDKKLRDTGIEVIGEAFWGTHIAQFYESKDDFFSIGCPYIRAGL
                     LNNELCVWVYSNNTNEEEIKSYLEKYINNADSYIKAGQLLLEPHVDWYLTNNSFNEIR
                     VNEHWLECVTKSTKLGYEGLRAIGDTSWLEKCYYRTFANYENKINKMLPELPFIALCL
                     YDENKTDKLQYAEVIKNHSYTIINNENKKELITNIELVIKDKQLNKALEYNKVKAEFF
                     ANISHELRTPLNVILGTVQMMELAKCTSYEKEEKYLKFLKQNSLRLVRLISNLIDITK
                     IDANYFEIHRKNLDIVNFAELITFSVLDYAKCKNIYIIFDTDIEEKVISCDPDYIERI
                     LLNLLSNVIKFTPRGGKILVSVHDMNERISISVKDNGIGIPVDKQKNIFNRFEKVDKS
                     LRRENEGSGIGLSLVKLLVNKLDGNIYLKSQPSKGSEFIVEIPCMTTPETSEFNIENT
                     SVNNIVERAKIEFSDIYC"
BASE COUNT         1347 a          532 c          684 g         1185 t
ORIGIN      
        1 taaagccagc tgtcttgcca tgttgattgc atcaccgtca tcgacgcgaa ttacagtatc
       61 cagttgctct aatttaacga tttcgggaat catcccgtcg cctattccat caattctatg
      121 aaagcctgaa accataccgc ctgatgacat ggcgggtgac tgtattggct caagagcgaa
      181 tgccttacaa ttggggtatt tttcttgcag agcatttttc acccccatga tggtccctcc
      241 tgtacctact cctgcaacaa aaccatccgg ttttttgcca attgcgtcaa gttgtcttaa
      301 aatttcagct cctgttgttt tataatgggt ttccgcattt aagagatttg aaaactgatg
      361 gggtaaaaaa gcattatttt cagctccgaa tttatcggct gattcgacag agcctttgaa
      421 accgccatct ttctttgaaa ccaagtggat tgttgcaccg aaactttcca atagtttttt
      481 tctttcctcg ctcatccaat ccggcataaa aatatgtact ggattttcta aaaatgctcc
      541 catggcagaa aatgcaattc cggtgttgcc gcttgatgcc tcagcaatta tatctccctt
      601 tttaattaag cctgcttcat aggagtattt taatatattg taagcaatcc tgtctttaat
      661 gctgccggta aaattgtagt attccgcctt agcgtatact tttcgccgga gtccttgata
      721 ttttaaagaa atttccagca tgggagtatt attaatcata gctgatattt tttcaaattt
      781 attcatgata ccatatcctt tttattatta taactaacat ttaaaataat ataatttata
      841 aattcaaatc acaaaatcca acaaaatatt tggaaatttt attttttttc gacatttttt
      901 ccttgttaaa attacaggat tggttataat tatataaaaa ggtaaaaaga aggattttat
      961 gcttattaaa atgccaaagg aagttgaaac tgcattaaat atattgcatg taaacggctt
     1021 tgaagcttat atagtagggg gatgcgtcag ggacagcatc atgggcaaag ttccttccga
     1081 ttgggatgtt gccacatcag caaagccgga agaaatactg ctgtgtttta gtcagtacag
     1141 aaccatagaa acaggtataa aacacggtac aattacagta attataaata aaatgcagat
     1201 tgaaattaca acttacagaa ttgacgggga gtacagtgac aacaggcgcc ccgataatgt
     1261 tttgtttacg gataaaattg aatttgattt aaagcggaga gattttacga ttaatgctct
     1321 tgcttataac aaaaatggag taatcgactt gttcggagga ctcgatgata taaaaaacaa
     1381 aattgtaaaa tgcgtgggga attcagatga aagattcaat gaggacggtt tgcggatttt
     1441 acgagcattg cgctttgctt ccgttttgaa ctttgacatt gatgagaata catcatgcag
     1501 tatttacgga aacaaaaatc tcctaaataa tatttctaag gaaaaaatta atattgaatt
     1561 aaataagctg ttaatgggaa gtaattttca aaatattctt ttggaataca gagatgtaat
     1621 tgaagttttt ctgcctgaaa ttagagattt aacaaaggaa aaatttgagc aacgtttaaa
     1681 ggcaatgaag aagttaaatt ctctggtttt gagactaact gtgcttttgt atgaaattga
     1741 ttcgcctgat aagatactta tgaatttaaa atatgacaac aagactgtta aaacagttaa
     1801 gttgcttgct gaaaatattg atgaaaaaat ttaccccgac cgggtgaata ttaagagatg
     1861 gctcaataaa atcaatcatg taaatctaaa atatcttatt aatattaaaa aggcattgtt
     1921 tgattatgag tatgaagagt tggccaagtc cgaagtaatc atgaaagaaa ttattgaaac
     1981 taaccagtgt tacagcttaa aaacattagc aatcaacggc caggacctta ttgattcagg
     2041 tatgccgaaa ggcaagaata taggaatagt attaaatgat gtattaaata aagttattga
     2101 aggcaaattg gaaaataaaa aagacatctt atttaattac atttatgata gaagaaagga
     2161 gattgaaaca ccgaattgac atgttaagta gaaaagtcta tgcaaatatt gaagtaatct
     2221 ggaatacctg ttaatcttcc taaattttta tttaaatata ttgttgataa atttgggagt
     2281 ataaaatgga taagaaactt agggatacag gtatagaagt aattggtgaa gctttttggg
     2341 gaactcacat agctcaattt tatgaatcca aggatgattt ttttagtatc ggttgccctt
     2401 atataagagc gggtttatta aacaatgaat tgtgtgtatg ggtttattca aacaacacaa
     2461 atgaagaaga aataaaaagt tatttagaaa agtatatcaa caatgcggat agttatatta
     2521 aggcagggca gttgttgtta gaaccacatg ttgattggta cttaaccaat aatagtttta
     2581 atgaaataag agttaatgaa cattggcttg aatgtgtcac aaaatctaca aaactaggct
     2641 atgaaggact tagagcaatt ggtgacacat cttggctgga aaaatgttat tacagaacct
     2701 ttgctaatta tgagaataaa ataaataaaa tgttgccgga attacccttc attgctttgt
     2761 gtttatatga cgagaataaa acggacaagc tgcaatatgc ggaagttatc aaaaatcaca
     2821 gttatacaat tatcaataac gaaaataaaa aagaattaat aacaaatatt gaattggtaa
     2881 ttaaggataa acaactaaat aaagcattgg aatacaataa agttaaggcg gaattctttg
     2941 ccaacatttc tcatgaatta agaactcctt taaatgtcat attagggaca gtacaaatga
     3001 tggagttagc aaaatgtaca tcttatgaaa aagaagaaaa gtatttaaaa tttttaaaac
     3061 aaaattctct aagattagta agactaataa gcaatttaat tgatataacg aaaattgatg
     3121 ctaattattt tgaaattcac agaaaaaact tagatatagt aaattttgcg gaattaatta
     3181 ctttttctgt tttagattat gctaagtgca aaaatattta tatcattttt gatacagata
     3241 ttgaagaaaa ggtaatatca tgcgacccgg attatattga acgaattttg ctgaatctct
     3301 tatctaatgt tattaaattt acgccgaggg gcggtaaaat attagtatcg gtgcatgata
     3361 tgaatgaaag aatcagtatt tcagtaaaag ataatggaat cggcataccg gttgataagc
     3421 agaaaaacat atttaacaga tttgagaagg tggacaagtc attaagaaga gagaatgaag
     3481 gtagcggaat aggcctctcc ttagttaaat tattagtaaa taagctcgat gggaatatat
     3541 atttaaaaag ccagccttcc aagggcagtg aattcattgt tgaaattccc tgcatgacaa
     3601 cacccgagac atcagaattt aatattgaaa acacctcagt caataatatt gtagaaaggg
     3661 ctaaaatcga gttttctgat atatattgtt aacggataaa gacttttact tgtccggacg
     3721 gagttattat cattcttgtg acgctaac
//
LOCUS       DAOSOW010000171         3741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_24657_length_3741_cov_3.069181, whole genome shotgun sequence.
ACCESSION   DAOSOW010000171 DAOSOW010000000
VERSION     DAOSOW010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24657_length_3741_cov_3.069181"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="PLD67_05315"
     CDS             <1..319
                     /locus_tag="PLD67_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HOT21972.1"
                     /translation="LNSLKIKKSSREYLSMEIGGHARECIGEAILAYKEGFDGAIQIF
                     PMGCMPEIVSKSILPTISKDYGFPIMSLVVDDMTGEAGYITRVEAFIDLLERRRDNVL
                     YGS"
     gene            300..1271
                     /locus_tag="PLD67_05320"
     CDS             300..1271
                     /locus_tag="PLD67_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOT21973.1"
                     /translation="MYYMGVDVGSVSTDIALIDNNYELIEKIYLRTKGNPIKAVQEGF
                     KILKNKYEDSEIYAVGTTGSGRMIASAIIGADVVKNEITAHATAALEIDKDVKTIIEI
                     GGQDSKIIIINNGIVVDFAMNTVCAAGTGSFLDRQAERLEIPIEEFGDYAMKASTGVR
                     IAGRCTVFAESDMIHKQQLGYNHAEIIRGLCDALVRNYLNNIGKGKTILPKVLFQGGV
                     AANKGMKKAFENELGFEIYVPENFSMMGAIGAAIIAANKVKKTGKTNFRGLKLADNNI
                     FSRSFDCEGCPNKCEVVKIYENDDIIGYFGDRCTKWSSKICEKYKLA"
     gene            1432..3369
                     /locus_tag="PLD67_05325"
     CDS             1432..3369
                     /locus_tag="PLD67_05325"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOT21974.1"
                     /translation="MLKEDDFPFLSARSYESTLNKIESAWENFIAGEIIPDFNLPRNP
                     ILESWKRSKEMGVPYNLKCAPLKLNEHDIMVERERQHNLFMIIDSYLDSIFKIFHDEN
                     MAVSFSNEKGIILSIRAGKSMLGNCHGINYLLGAGWSENDCGTNSIGTSLYTGKPIQI
                     YATEHYCKMAHSINCSSAIIKEPSSEIVLGALTITTHKNIVPAHTLNWAISEAQKIEN
                     AIEYHLQEESTSLMNCLFEMNEQPCIICKLDGKISKINKSAQMLGVKVGDRIENSFVF
                     PKYSRFDELFDNTYKTICCKTEQRYIVTIIPWRIGNNIIGAIAYFQKESLFLNQGNSA
                     KLTSDRSEFKCIIGKSPAFRQVIDLADKFSDSDMPILITGETGTGKEVLARAIHNNSC
                     RKEKIFLPVNCGAIPKELISSELFGYVEGAFTGTKKGGRIGKIEASSGGTLFLDEICE
                     MPIDMQIYLLRVLEEGTITRVGSNEAIPVNLRIICATNKDIRHEVEIGNFRKDLYYRL
                     NPVELHLPTLRDRGDDIILLAEHFLKLNSQDFKLSADAAELLMNYSWPGNVRELKSVI
                     NRAILLSKNSIITSNELFIHDKKQEIKYSKTHTEINEKRPTSDLIKQVIEECGGNKSL
                     AARRLKISRMTLYRRIKEYNL"
BASE COUNT         1375 a          574 c          742 g         1050 t
ORIGIN      
        1 attaaattct ttaaaaataa aaaaatcatc ccgggaatat ttatccatgg aaataggagg
       61 tcatgccagg gaatgcatag gcgaagcaat ccttgcatat aaggaagggt ttgacggggc
      121 aattcaaatt ttccctatgg gttgtatgcc tgaaatagta tctaagtcaa ttcttcctac
      181 tatttcaaag gattacggct tcccgataat gtcattggta gttgatgata tgacaggaga
      241 agcaggttat attacgagag ttgaagcatt cattgattta ttggaaagga gacgcgacaa
      301 tgtattatat gggagttgac gttggctctg taagtacgga tattgctctt attgataata
      361 attatgagtt gattgaaaaa atatatttaa gaacaaaagg aaaccctatt aaagcagtgc
      421 aggaagggtt taaaatacta aaaaacaaat acgaagactc tgaaatttat gcagtgggaa
      481 ctacgggaag cggcagaatg attgcttcag caattatcgg tgcagatgtc gtaaaaaatg
      541 aaattaccgc tcatgcaaca gcagcccttg aaattgacaa ggatgtaaaa accataattg
      601 aaataggcgg acaggattca aaaataataa ttattaataa cggaatagtt gttgactttg
      661 ccatgaatac cgtctgcgcc gccggtaccg gctcattttt agacaggcag gcggaaaggc
      721 ttgaaatacc tatcgaagaa tttggcgatt atgcaatgaa agcaagtacc ggtgtaagaa
      781 tagcaggcag gtgtaccgta ttcgccgaat cagacatgat acataagcag cagcttggct
      841 acaaccatgc agaaataatc agaggactat gtgatgcact ggtgagaaac tatttaaaca
      901 atataggcaa aggcaaaaca atacttccaa aggttttgtt tcaaggagga gttgcagcaa
      961 acaaaggtat gaaaaaagct tttgaaaacg agctgggatt tgaaatatat gttcccgaaa
     1021 acttcagtat gatgggagcg ataggagctg caattattgc cgctaataaa gtaaaaaaga
     1081 caggcaaaac aaatttcaga ggccttaagc ttgccgataa caatatattt tcaagaagct
     1141 ttgattgtga agggtgcccg aataaatgtg aagtggtaaa aatttatgaa aatgatgata
     1201 taatcgggta tttcggtgac agatgcacaa agtggagcag taaaatatgc gaaaaataca
     1261 aattagcata aaatatcagt caaaggatat catcacttgt tactgccaaa tctcaacgtg
     1321 cttatgatgc aaagaatgct ccatttcatc cgtattgaca gggtatataa aaaattgtat
     1381 aatcaattat aatccgtaat aaaaatttaa gtgaaataag gtgttaaacc tatgcttaaa
     1441 gaagatgatt ttcctttcct gtcagctcga agctatgaat caacattaaa taaaatcgaa
     1501 tcagcatggg agaatttcat tgccggtgaa attatccctg attttaactt gcctcgaaat
     1561 cccatcttag aatcatggaa acggtcaaag gaaatgggag ttccatacaa tcttaaatgt
     1621 gcacctttga aattaaatga acatgatata atggttgaaa gagaacgaca gcataactta
     1681 ttcatgatta tagattcata cctggatagt atattcaaaa tatttcacga tgaaaatatg
     1741 gcagtttctt tcagcaatga aaagggtatt attttgagta tcagagccgg aaaatcaatg
     1801 ttagggaatt gtcacggcat taattatttg ttgggagccg ggtggagtga aaacgattgc
     1861 ggtactaata gtataggcac atctttatat acaggcaagc caatacaaat ttatgcaaca
     1921 gagcattatt gcaaaatggc acattcaata aattgttcat ctgctattat aaaagaacct
     1981 tcttctgaaa tcgtattagg agcattaact attacaacac acaaaaatat tgttcccgcc
     2041 catacgctta actgggctat cagcgaagca cagaaaatag aaaatgcaat tgaatatcat
     2101 ttacaagaag aatcaacgtc tttaatgaat tgtttgtttg aaatgaatga gcagccttgc
     2161 ataatctgca agcttgacgg taaaatcagt aaaataaaca aatcggcgca aatgttaggt
     2221 gtaaaagtag gggatagaat agaaaacagt tttgtatttc ctaaatactc ccgatttgat
     2281 gaactgtttg ataatactta taaaacaatt tgctgcaaaa ctgagcaaag atatattgtt
     2341 actatcattc cttggcgtat aggtaataat attataggag caatagcata tttccaaaaa
     2401 gaaagtttat ttcttaatca aggcaactct gctaaattga cgagtgatcg cagcgagttt
     2461 aaatgtataa tcggtaaaag tccggcattt agacaagtaa ttgatttagc tgataaattt
     2521 tcagattctg acatgcccat actgataaca ggtgaaacag gaacaggcaa agaggtattg
     2581 gctcgtgcta ttcataataa cagctgccga aaagaaaaaa tctttctgcc ggtaaactgc
     2641 ggagcaatac ctaaagagtt aatatcttca gaattattcg gatatgttga aggtgctttt
     2701 accggcacta aaaaaggcgg aagaataggt aaaattgaag catcaagcgg aggcaccctg
     2761 ttccttgacg aaatctgtga aatgcctatt gatatgcaga tttacctttt aagagtattg
     2821 gaggaaggta caattacccg agtaggttca aatgaagcga taccggttaa tttacggata
     2881 atatgcgcta ctaataagga tataagacat gaggtagaaa tagggaattt tcgaaaagat
     2941 ctttattaca gattaaatcc tgtagagctt caccttccga ctttaagaga tagaggtgat
     3001 gatattatat tgcttgctga acattttttg aaactaaatt cacaagattt taagttatca
     3061 gcagatgctg ctgaattgtt aatgaattat tcgtggccgg gaaatgtacg tgaattaaaa
     3121 agtgttatta atcgtgcgat tttacttagc aaaaattcaa taattacatc aaacgaactg
     3181 tttattcacg ataaaaagca agagataaaa tattcaaaaa ctcatacgga aataaatgaa
     3241 aaacgtccga catccgattt aataaagcaa gttattgaag aatgcggcgg caataaaagt
     3301 cttgccgcac gcaggttgaa aatttcaaga atgacattat acagaagaat taaagaatat
     3361 aatttataat gactatgtga tgcttggtca taagtatagt tgcctaacag gcaacttttt
     3421 tattaatgtt acatattacc aagtgacaga tgttacaatg ttacataaaa agtgacacca
     3481 atacaatgaa aaaccgcaac aattaacaca tgtttcttgg cataaaaaat gctaattatt
     3541 aatagaatgt ttgtttaaat tttcataaac aacatcagta aacattatta attattatct
     3601 aaatggaggg aagcatgaaa ataacaaaat tgatttcatt attgctggct ttactaatga
     3661 ttacttcatt agcagcatgt acacagcctg cggcagaaac tccgccggaa gaaccacccg
     3721 cggaagaaac tccgcctgca g
//
LOCUS       DAOSOW010000172         3731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_24738_length_3731_cov_3.909956, whole genome shotgun sequence.
ACCESSION   DAOSOW010000172 DAOSOW010000000
VERSION     DAOSOW010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24738_length_3731_cov_3.909956"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..630)
                     /locus_tag="PLD67_05330"
     CDS             complement(79..630)
                     /locus_tag="PLD67_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT21975.1"
                     /translation="MIFNEKTVTLKNGKVCVLRSPKASDAEEMLEYLKKTAAETDFLL
                     RYPEEVVMTIEEEIDFINFVRDNPRGIMICAFIDDKLVGTTHLMGLGDRFKISHRASF
                     GIAVLKEAWHLGIGNALITEMLKIAEDTGFELVELEVASTNHNAIVLYKKFGFKIYGT
                     RENGFKFKDGTYCDEHLMLKRIK"
     gene            complement(690..1037)
                     /locus_tag="PLD67_05335"
     CDS             complement(690..1037)
                     /locus_tag="PLD67_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HOT21976.1"
                     /translation="MNKRFISLIVLLILAIVIVAVINHFILNNGETINKVSFNATVLE
                     NYGNSILVNPLEGSPELNSSDKIVVRVPVDNAALKDLSEFTVGSKVKITYDGMIMESY
                     PAQINAYNVEFAK"
     gene            complement(1106..2221)
                     /locus_tag="PLD67_05340"
     CDS             complement(1106..2221)
                     /locus_tag="PLD67_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010676765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOT21977.1"
                     /translation="MNRKKYELAVQLRHELHQHPELSNNEVWTKKHLIKFLKEHTKLE
                     IVDRGSWFYAVYKSAHGKKNTAFRADFDAVPIDEYIDIPHGSKVQGVSHKCGHDGHSA
                     SLAGFALEIDRYGADNNVYFLFQHAEETGDGAKEAAVFITENNIDEIYAYHNMSGIPF
                     KAVAVKNGVSFCASKGMIIHMEGIPSHASMPEKGRNPSFAIANIINSVPEFTSPDNYK
                     GMVLCTVIQVAIGERAFGTSASKGELLLTIRALYEEEMNMLQKKIEELAKNEAKKYGL
                     KVNFEYVDVFPETYNHGENVDKIRKAAKNIGYELIEMKEAIRSSEDFGYYTKSTKGAI
                     FLIGNGEDYPAVHINEFDFRDELIEVAADLFKEILLH"
     gene            2540..3502
                     /locus_tag="PLD67_05345"
     CDS             2540..3502
                     /locus_tag="PLD67_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HOT21978.1"
                     /translation="MRRILITGALGQIGSELTEEMRKIYGSDNVVASSRRIKEGHEKL
                     IESGPFEIINVNDPKTLASAVDKYKINTIVNLAAVLSAVGEKEPQQCWNINMNGLYNI
                     LEIAKEKNCMVYTPSSIAAFGPSTPKDNTPQDTIQRPTTMYGVTKVAGELLCDYYYKR
                     FGVDTRGVRFPGLISYKTLPGGGTTDYAVHIYYEALKNKKYTSFINKGTYMDMMYMPD
                     ALRAIIDLIEANPDRLKHRNAFNVSAMSFEPEMLAESIKKFIPEFKLEYDVDPVRQEI
                     AESWPNSLDDSDAREEWDWNPQYDLEAMTKDMLEKLRIKLNIQE"
BASE COUNT         1183 a          702 c          584 g         1262 t
ORIGIN      
        1 attattgtca tcctgatcaa tagctaagga ttctatcttt aatgtattag ttcttcacat
       61 acgttcagga tgacacggtt attttatcct tttcagcatc aaatgctcat cacaatatgt
      121 cccgtcttta aatttaaaac cgttttctct tgtgccgtag attttaaatc caaatttttt
      181 atatagcact attgcattgt gatttgtgct tgcaacctct aactcaacca gttcgaaccc
      241 ggtatcctct gctatcttta acatttcagt tattagtgca tttcctattc ctaaatgcca
      301 tgcttccttc aaaacagcta taccgaaaga tgctctgtga gagattttaa acctgtcacc
      361 aagacccatt aagtgtgttg ttccgacaag cttatcgtca ataaaagcac aaatcattat
      421 accccttgga ttatctctta caaaatttat aaaatctatt tcttcttcaa ttgtcattac
      481 tacttcttca ggataacgca acaaaaagtc tgtctcagct gccgtcttct ttaaatactc
      541 aagcatttcc tctgcatcag atgcttttgg acttcttaat acacatactt tcccattttt
      601 taatgtaaca gttttctcat tgaatatcat aattaaccac cttcgaattt aatcgatttc
      661 ctccacataa tggggaggat gatataattt tattttgcaa actcgacgtt ataggcattt
      721 atttgtgcag gatagctttc cataatcatt ccgtcataag taatttttac tttgctgcca
      781 acggtgaatt ccgataaatc ttttaaagca gcattatcta caggaaccct aacaacaatt
      841 ttatcgcttg aattaagctc cggtgagcct tctaatggat ttaccagtat tgaattgccg
      901 taattttcaa gtacagttgc attaaaagat actttgttaa ttgtttcacc gttatttaat
      961 ataaaatggt ttataacagc aactattacg attgccaaaa ttaataatac tatcaaagat
     1021 ataaaccttt tgttcatttt tatcacctca agtatataga cgttattaat aaagaaatgt
     1081 tccaattata ctcacttacg ttcctttaat gcaacaatat ttccttaaat aagtctgcag
     1141 caacttcaat cagttcatcc ctgaaatcaa attcgttaat gtgaacagca gggtaatctt
     1201 ctccattacc tattaaaaag atggcacctt tggttgattt tgtgtaatac ccgaaatctt
     1261 ccgagcttct gattgcttcc ttcatttcta tcaattcata tcctatattt tttgctgcct
     1321 tccttatttt gtccacattt tcaccatgat tatatgtttc ggggaaaaca tcaacgtatt
     1381 caaagtttac ctttagtcca tactttttag cttcattctt cgccagttcc tcaattttct
     1441 tctgcagcat attcatttct tcttcatata aagcgcgaat agtcagcaag agctcaccct
     1501 tgcttgcact tgtaccaaaa gctctctccc ctatagctac ttgtattact gtacaaagga
     1561 ccattccttt ataattatcg ggagaagtaa attccggaac tgaatttatt atatttgcaa
     1621 tagcaaagga tggatttctc cccttctccg gcatgctggc atgtgaagga ataccttcca
     1681 tatgtattat catgcccttt gaggcacaaa aactaactcc gtttttcacg gcaacagctt
     1741 taaaaggtat gccgctcata ttgtgataag catatatttc atcaatatta ttttcggtta
     1801 taaatacagc tgcttctttt gctccatctc cggtttcttc agcatgctga aataaaaagt
     1861 agacattatt gtcggccccg tatcggtcaa tttccagtgc aaaacctgct aatgatgcag
     1921 aatgtccgtc atgtccgcat ttatgcgaaa caccttgaac tttagacccg tgaggaatat
     1981 ctatatattc atctatcggg actgcatcaa agtctgcacg aaatgctgta tttttctttc
     2041 cgtgggctga tttatataca gcatagaacc agctgcctct gtccactatt tctaattttg
     2101 tatgttcttt caaaaacttt atcaaatgtt tttttgtcca gacttcatta ttggaaagct
     2161 caggatgttg atgtaattca tgtcgtaatt gcactgctaa ctcatacttt ttcctattca
     2221 tgtctctcct atggaatttt ggggtagacc ttaactccgc ctaaattcgg cgattccgcg
     2281 ggcgtacctt gaaattcctc taattcccat tcataacatt ttgactatta gataataaca
     2341 ttttctcaat tcaatatcaa ttataaatat taacctttta taatcaatca gataatcgtc
     2401 ctctatttgg agacaattct tattttttgt tgaataaaat agtatttttt agaaaacttt
     2461 tgtttatatt ttataagttt tatgttatta tatatacata attatatagt atcatagtat
     2521 atttatggga ggtaattaat tgagaaggat tttaataaca ggggcattgg gtcaaatagg
     2581 ctccgaatta actgaagaaa tgcgaaagat ttacggttca gacaatgtag tagccagcag
     2641 cagaagaata aaggaaggac atgagaaact aattgaatca ggtccatttg aaatcataaa
     2701 tgtcaatgac ccgaaaacat tggcatcagc ggtagataaa tataaaataa acaccattgt
     2761 aaaccttgca gccgtgttgt ctgccgttgg agaaaaagaa cctcaacaat gttggaatat
     2821 taatatgaac ggtctgtaca acatcttaga aattgccaaa gaaaagaatt gcatggttta
     2881 cacaccatct tccattgcag cttttggtcc ttcaacccca aaggacaata ctccccaaga
     2941 tacaattcaa agacccacaa ccatgtatgg agtaactaaa gttgccggtg aattattatg
     3001 tgactattat tacaaaagat tcggtgtaga cactagaggt gttcgtttcc cgggacttat
     3061 ttcatacaag acgttaccgg ggggaggaac aactgattac gcagtacata tttactatga
     3121 agctttgaag aataaaaaat acacaagctt tatcaataaa ggaacatata tggatatgat
     3181 gtatatgccg gatgcactaa gagcaataat tgacttaata gaagcaaatc cggacagact
     3241 taaacaccgc aatgctttta acgtttcagc catgagtttt gaacctgaaa tgttggctga
     3301 atctataaaa aaatttatac ctgaatttaa gcttgaatat gatgttgatc cggtaaggca
     3361 ggaaatagct gaatcatggc ctaattcctt agatgattcc gatgcgagag aagaatggga
     3421 ttggaatccg caatatgact tagaagcaat gacaaaagat atgcttgaaa aattaagaat
     3481 taaattaaat atacaagaat aatttatact ttgggacatt ccttcaagga atgtcccaaa
     3541 tttttgatga atgcttaccg tgaattaact caattttata ttgcaaaaat tttattaaaa
     3601 tgataaattg taattaagaa ttttctaaca attgaaaagg agaaaacaat atggatgtta
     3661 agtatgtagc agaaccgttt agaattaaga tggtagaacc actcaaaatg ttatctcgcg
     3721 aagagaggga g
//
LOCUS       DAOSOW010000173         3718 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_24845_length_3718_cov_3.591592, whole genome shotgun sequence.
ACCESSION   DAOSOW010000173 DAOSOW010000000
VERSION     DAOSOW010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24845_length_3718_cov_3.591592"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLD67_05350"
     CDS             complement(<1..586)
                     /locus_tag="PLD67_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21979.1"
                     /translation="MARGGGSFGGGGRGGGSFGGSRGGGSFGGSRGGGSFGGRSGGSF
                     GGRSGGSFRGSSSGGIFGSSKSTGTGFGPVFGPRIPMGGGFGGGRPQRSSGGGGCGCV
                     TLIVVLIVIAVIFIVIFSLWGSMNSSGGGDITFSSVKRDPLPKGSVNETEYYTDRLGW
                     INNETKLISGLKHFYEKTGVQPYLYLTDTINGSHF"
     gene            complement(621..1445)
                     /locus_tag="PLD67_05355"
     CDS             complement(621..1445)
                     /locus_tag="PLD67_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT21980.1"
                     /translation="MDKYEEIERSITKRFRKLIWAKFISAIKEYKLIQDNDKIAVCIS
                     GGKDSMLMAKCMQQLQKYSEIHFKTEYLVMDPGYNDANKKRIIENAEILNIPIKIFNT
                     PIFEIVDRVEETPCYLCARMRRGYLYRNARELGCNKIALGHHFDDVIETVLMSLFYAA
                     EIKTMMPKLHSTNHEGMELIRPMYKVREDDIIAWKRYNNLQFIQCACRITERIKAEGT
                     GSKRQEMKILINKLRQINPNIDTNIFKSIHNVNLDTIIGYRKGNVYRNFLDDYDKN"
     gene            1607..2410
                     /locus_tag="PLD67_05360"
     CDS             1607..2410
                     /locus_tag="PLD67_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HOT21981.1"
                     /translation="MGIAHEVDYKIYGDDIQFVEIGLDYGETIVAEPGAMMYMDSSIE
                     MDTMFGDGTEKSKGFKGMLATAGKRVLTGENLFTATFTNTSIDKKVVAFAAPYPGKII
                     PVNLPDYGGRLICQRDAYLCSAKGIEITIAFQKKIGAGLFGGEGFIMQKLVGDGFAFL
                     HAGGTIVKKELASGEMLKLDTGCLVAFTEGVDYDVQFASNVKSALFGGEGLFLATLRG
                     PGTVWMQSLPFSRIADRIYSASRKSGGGGSKGEASLLGNIGIGNLFGGD"
     gene            complement(2468..3373)
                     /locus_tag="PLD67_05365"
     CDS             complement(2468..3373)
                     /locus_tag="PLD67_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HOT21982.1"
                     /translation="MKYLIGIDSGGTKSELTAYDLNNAQVFNLIGEFGNPAVNLDKTV
                     NNLISLIYDCVNELGREECQLIAVGMAGVETGNYAELIKKYVEGAFVIKTIVLNDAEM
                     ACKAYFGDKDGILAISGTGSSCFAQKDGAGEMVGGWGHILGDEGSGYHTVINVIKNIV
                     YKYDRALPFDDLSKAVMEEIGGSTRSDIMNFIYNNEKNKIASLFPIISDYAEKGDAYA
                     TMLLENAGKYLAELTSVAYKKKNFKGTANIGLKGGVFHNSSIMLSAYKDEIKKRLDSY
                     EIIEEDISTTKAVCNIFKNEKEGAR"
     gene            complement(3532..>3718)
                     /locus_tag="PLD67_05370"
     CDS             complement(3532..>3718)
                     /locus_tag="PLD67_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOT21983.1"
                     /translation="RANVDRDACISCGLCESICPDVFELDDEGISVVKVDPVPSEFED
                     CAKEAEEECPTDAIHVE"
BASE COUNT         1090 a          782 c          634 g         1212 t
ORIGIN      
        1 gaaaatgaga accgttaatc gtatccgtca aatataaata tggctgaact cccgttttct
       61 cataaaaatg ttttaagccg ctgataagtt ttgtttcatt attaatccaa cccaggcgat
      121 ccgtatagta ctccgtttca tttacagacc cctttgggag cggatctctt ttaacagatg
      181 aaaaagtaat gtcgccgccg ccgcttgagt tcatggagcc ccataatgaa aaaattacaa
      241 taaaaataac cgcaatcaca attaaaacaa caattaatgt aacgcatccg cagcctccgc
      301 caccgcttga cctctgcggt ctaccgccgc cgaatcctcc gcccatgggg attctgggac
      361 caaataccgg accaaatccg gtaccggtac ttttactgct gccaaatatt cctccgctgc
      421 ttgagcctct gaatgagccg ccgctgcgtc caccaaatga gccgccgctg cgtccgccaa
      481 aagacccgcc gccacggcta ccgccaaatg aacctccccc gcgactaccg ccaaatgagc
      541 cgcctccacg gccgccgccg ccgaatgaac ctccgcctct agccaatgaa atctcctcca
      601 attatatcaa agatactgta ttaattttta tcataatcat caagaaaatt acggtacaca
      661 ttcccttttc gatagccgat aattgtatct aaattcacat tgtgaatgct cttaaatata
      721 tttgtatcga tattcggatt gatttgtctt aacttattta ttaatatttt catttcctgc
      781 cttttagaac ctgtaccctc agccttaatt ctttccgtaa tgcggcaggc acattgaata
      841 aactgcaaat tattgtatct tttccaagca attatatcat cttcccggac tttatacata
      901 ggtcttatca gctccatacc ctcatgattt gtactgtgaa gtttgggcat cattgttttt
      961 atttctgcag cataaaataa gctcattaga actgtctcaa ttacatcgtc aaaatggtgc
     1021 cctaaggcta ttttattgca gcctaattct cttgcatttc tgtacaaata acctcttctc
     1081 attcttgcgc ataaataaca cggggtttct tcaactctgt ctactatttc aaatattggt
     1141 gtattaaaaa tttttattgg gatatttaat atttctgcat tttcaatgat acgttttttg
     1201 ttagcatcat tgtatcccgg gtccattacc aaatattctg ttttaaaatg aatttcactg
     1261 tatttttgaa gttgctgcat gcatttagcc ataagcattg aatccttgcc gcctgatatg
     1321 cacactgcaa ttttgtcatt gtcttggatt aatttatatt cttttattgc tgatataaat
     1381 tttgcccata ttaattttct aaaccttttt gtgatacttc tttcaatttc ctcatattta
     1441 tccattttgt cctgcttaat aatatgatag taataataat agttatactc tattatatta
     1501 acataatcta cataaaacaa gatattttta tattgcattt catgaaaatg tttgctatcc
     1561 taatattgga tttaggggtt aaatactttt taaacaagga ggatttatgg gaatagctca
     1621 cgaggtggat tataagattt acggagacga tattcagttt gtagaaatcg gtctcgatta
     1681 cggggaaact atcgttgcag agcccggagc aatgatgtac atggattcaa gcattgaaat
     1741 ggatactatg tttggagatg gcactgaaaa aagcaagggc tttaagggaa tgttggcaac
     1801 agcaggcaaa agggttctta ccggagaaaa tttatttact gcaacattta ctaacacgag
     1861 tattgataaa aaggtagttg cttttgcagc accctatccc ggaaaaataa tacctgttaa
     1921 tttgcctgat tacggcggac ggcttatttg tcaacgagat gcctatttat gttctgcaaa
     1981 agggattgaa ataaccattg cattccagaa aaaaatcggt gcaggacttt tcggcggtga
     2041 aggatttata atgcagaaat tggttggaga tggttttgca ttccttcacg caggcggcac
     2101 aattgttaaa aaggaattag cttcaggaga aatgcttaaa ttagatacgg gatgtttggt
     2161 ggcatttact gaaggagtag actatgatgt tcaatttgca agcaatgtaa aaagtgcctt
     2221 gtttggcggc gaaggtttat tcctggctac attaagaggt cccggaactg tatggatgca
     2281 gtcattgcca ttcagcagaa tagctgacag aatttattcg gcaagcagaa aaagcggcgg
     2341 aggcggctca aaaggagaag caagtttact tggaaatatt ggaataggta acttattcgg
     2401 tggtgattaa agatgcccta accctatttg ttagaggtat ggggatacac attttttctg
     2461 aaggtgctca gcgagcacct tctttttcat tcttaaatat attacaaact gccttggttg
     2521 ttgaaatatc ttcttctatt atttcataag agtccagcct tttttttatt tcatccttat
     2581 atgctgaaag catgatagag ctgttgtgga aaactcctcc ttttagacct atattagcag
     2641 ttcccttaaa atttttcttt ttatacgcca ctgatgtaag ctcagccaga tatttgcctg
     2701 cattctctaa gagcatggtt gcataagcat ctcctttctc agcataatct gaaattatgg
     2761 gaaaaagcga tgctatttta tttttttcat tattatatat aaaattcatt atatccgagc
     2821 gtgtagagcc gcctatttct tccataacag ccttgcttaa gtcatcaaag ggaagagccc
     2881 tgtcatattt ataaactata tttttaataa catttataac cgtatgataa ccgcttcctt
     2941 catcgcccaa aatatgtccc catcctccaa ccatttcacc tgctccgtct ttttgagcaa
     3001 agcaagaaga ccctgttccg gaaattgcca gaattccgtc cttatctcca aaatatgctt
     3061 tgcaagccat ttcggcatca ttcaatacta tagtcttaat tacaaaagca ccttcgacat
     3121 attttttaat cagctcagca tagtttcctg tttcaacacc tgccattccg acagctataa
     3181 gctgacattc ttctcttcct aattcattaa cgcagtcata aattaatgaa attaagttgt
     3241 tgacagtctt gtccaagttt accgcaggat taccaaattc tcctattaag ttgaaaacct
     3301 gagcattatt aaggtcataa gccgtaagct ctgactttgt tccgcccgaa tcaattccaa
     3361 ttaaatattt catactctca ccacccgata ttagtatagc aaaaactact ttatttttcc
     3421 atataaatta taaatattat taattattta tagttaaaac aactcatcaa taaagattat
     3481 atgaaataat aaaactgctc ctctaatcta ggggagcagt cttgcataca tttattccac
     3541 atgaattgca tctgtagggc actcttcttc agcttctttt gcacaatcct cgaattctga
     3601 aggtacgggg tcaaccttta ctacagagat accttcatcg tccagttcga aaacatccgg
     3661 gcaaattgat tcacacagcc cgcagcttat gcaagcatct cggtcaacat tagctctc
//
LOCUS       DAOSOW010000174         3710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_24904_length_3710_cov_3.609576, whole genome shotgun sequence.
ACCESSION   DAOSOW010000174 DAOSOW010000000
VERSION     DAOSOW010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24904_length_3710_cov_3.609576"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      15..165
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            338..1234
                     /gene="dapA"
                     /locus_tag="PLD67_05375"
     CDS             338..1234
                     /gene="dapA"
                     /locus_tag="PLD67_05375"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOT21984.1"
                     /translation="MNDLVRGCGVALVTTFADGEIDYEVLGKLVEMHVENSGSIIVAA
                     TTSEAATMTDEEYHTVVEFVIDRVNKRIPVIAGAGSNCTQAAVKKAQFCEEAGADGLL
                     IVTPYYNKTTQRGLVNHYKIIAESTSLPIIVYNVPARTGMNIEPKTVLELSKIKNIVG
                     IKEASGDISQVAEIIRICPDDFLVYSGNDDMVVPLMSLGGHGVISVSANILPAEMKKM
                     VDDYRNGNVNDAKNAQLALNGINKALFIETNPIPVKEAMSIMNLCSSEVRAPLYQMSE
                     ENRARLLRELNLFGIGVPLETA"
     gene            1243..1911
                     /locus_tag="PLD67_05380"
     CDS             1243..1911
                     /locus_tag="PLD67_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOT21985.1"
                     /translation="MNVVICGINGAMGRVLQEEIKTYDNINLIGSLSPRSGRFGQDID
                     GMPDVVIDFSNTANIDFLIEYATKNKCALLICTTGFSDEDKAKIKEAGKAIPVLLSSN
                     TSLGINVFRKILKSISAELSTFDINIVEKHHTKKIDAPSGTAKTLSEDIKNATGREHV
                     EISAIRAGTIPGEHTVIFSGVDEILEIKHTAFSKKIFARGAINLAMKLSSKSPGYYLM
                     EDFF"
     gene            1938..2921
                     /locus_tag="PLD67_05385"
     CDS             1938..2921
                     /locus_tag="PLD67_05385"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HOT21986.1"
                     /translation="MNKIRIGIVGYGNIARGVEAAVNLSTDMELVAVFTRRQPDTIKT
                     QTDVKVLSVDEVEKYKDIIDVMVLCLGSATDLPVQAAEYAKMFNTVDSFDNHKKIPEY
                     LRKVDEGALSSGKISIISIGWDPGLFSIMRMLSESVLPEGSSYTFWGRGVSQGHSDAI
                     RRIPFVKQAIQYTIPVEDAVNCVRRGDNPELTAREKHIRQCYVVCDECADKNKIEEDI
                     KNMPDYFAEYNTEVNFISEEEFKKNHSRMSHGGLVIRNGMTGSEKNQHIMEFSLKLDS
                     NPEFTGSVILSYARAAYRMNKEGIKGAKTIFDVPLSYIHPEGREKLIRNFL"
     gene            3017..>3710
                     /locus_tag="PLD67_05390"
     CDS             3017..>3710
                     /locus_tag="PLD67_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOT21987.1"
                     /translation="MLKVAKFGGTSLADSKQFNKVYDIIKNNDERKYIIVSAPGKRFK
                     DDNKITDLLYLTYAHIKYSVPCAPVLKIIEDRFRQIKEELNLKTDLDYEFKIIKENLD
                     NKCEEDYIVSRGEYLCALLMADYLDCDFIDAKDVIFFNYDGTVNEEKTHEKLTEILGK
                     SNKAIIPGFYGSYPDGSIKTFSRGGSDITGSIVSSVMGADIYENWTDVSGFLVADPRI
                     VNNPKQIKKITYQ"
BASE COUNT         1348 a          560 c          806 g          996 t
ORIGIN      
        1 aaatattttc atgggcatat tacagaggtc gcgatgatca agagtagaag cttttcttcg
       61 aaagggtgat tcgccgaagg gttaatccct gggctcacat ttaatagatg tgatactgtt
      121 tttataggat gcatcccaat cctataaaag agcgctgtaa tatgttgggc agaaggttcg
      181 atgtttttta agcgtctgag agcccttttg ctcttatgtc gcttttttta tacctcaata
      241 tttgtacaag ctccggcgag gtacagacgg atggtaggtc atagggagcg acatgaaatt
      301 attttattta taaaaaataa tttaaaggag taagaagatg aatgatttag taagaggatg
      361 cggagtagca ttggtgacca cctttgctga cggagaaatt gattatgagg tcttaggaaa
      421 attagttgag atgcatgtgg aaaattccgg ttcaataata gttgcagcaa caacttctga
      481 agcagcaaca atgacagatg aagaatatca cacagtagtt gaatttgtaa ttgacagagt
      541 caataaaaga attcctgtta ttgcaggagc cggaagcaat tgtacacaag ctgcagtaaa
      601 aaaggcacag ttttgtgagg aggcgggagc tgacggccta ttgatagtaa ctccctatta
      661 caacaaaaca actcaaagag gacttgtaaa tcactataaa ataattgcag aaagtacatc
      721 actgccaatt attgtttaca atgtgccggc cagaacagga atgaatattg aaccaaaaac
      781 agtcttagag ctttcaaaaa tcaagaatat agtaggaatt aaggaagcaa gcggagacat
      841 cagccaggtt gcggaaataa tcagaatatg tccggatgac tttctggttt attcaggcaa
      901 tgacgatatg gtagtacccc ttatgtcatt aggaggacat ggagttattt cagtatctgc
      961 aaatatactt ccggcagaaa tgaagaaaat ggttgatgat tacagaaacg gaaatgtaaa
     1021 tgatgcaaag aatgctcagc ttgcactaaa tggaataaat aaagcattgt ttattgaaac
     1081 aaatcccatt cccgtaaaag aagcaatgag tattatgaac ttgtgcagct ctgaggtaag
     1141 agcaccctta tatcagatgt cggaagaaaa cagagcaaga cttctaagag aattgaattt
     1201 attcggaata ggcgttcctt tagaaaccgc gtgaggtgga gtatgaatgt tgtaatttgc
     1261 ggaataaacg gagctatggg aagagttttg caagaagaga taaaaacata tgacaatatt
     1321 aatctaatcg gctccctttc accaaggagc gggagatttg gacaagatat agacggtatg
     1381 cctgatgtag taatagactt ttcaaacact gcaaatattg attttttaat tgaatatgca
     1441 acaaaaaata aatgtgcatt attgatttgc acaacaggct ttagcgatga ggataaagct
     1501 aaaataaaag aagcagggaa agcaattcct gtactgttat cttcaaacac ttcattagga
     1561 atcaatgttt tcagaaaaat attaaaaagt atctctgcag agctaagtac ttttgatatt
     1621 aacattgttg agaaacacca taccaaaaaa atagatgctc ccagcggaac agcaaaaaca
     1681 ttgtcggaag acattaaaaa tgcgacagga agggagcatg tggaaatcag tgccataagg
     1741 gcaggaacca tccccggaga gcatacggtt atattttccg gagtagatga aattttagaa
     1801 ataaagcata ccgctttttc aaaaaaaata tttgctcgcg gagctataaa cttggctatg
     1861 aaactttcat caaaaagccc cggttactac cttatggaag atttctttta acaaaaataa
     1921 agacagcgga gggaaaaatg aacaaaataa gaataggaat tgtgggatac ggtaatatag
     1981 ccagaggagt ggaagctgcg gtaaatttaa gtacagatat ggaattagtg gctgtcttca
     2041 ccagaagaca gcctgatacg ataaaaactc agactgacgt aaaagtatta agtgttgatg
     2101 aggttgaaaa atataaagac ataatagatg ttatggtctt gtgtttaggc tcggcaacag
     2161 acctacccgt acaggcagct gaatatgcaa aaatgtttaa tactgttgac agctttgata
     2221 atcataaaaa aattccggaa tacttaagaa aagttgatga aggtgctctt tcttccggca
     2281 aaataagcat aatttcaatc ggctgggacc cgggactgtt ttcaattatg aggatgctgt
     2341 cagagtcagt cctgccggaa ggcagcagct ataccttttg gggaagaggg gtcagccagg
     2401 gccattctga tgctattcgc aggattccgt ttgtaaaaca ggcaattcaa tacacaatac
     2461 ctgtggagga tgcagtaaat tgcgtaagac ggggagataa ccccgaactt actgcaagag
     2521 agaaacacat aagacaatgc tatgtggttt gtgatgaatg tgcagataaa aataaaattg
     2581 aagaagatat aaaaaacatg ccggattact ttgcagaata taatacagaa gttaatttca
     2641 taagcgaaga agaatttaag aaaaatcatt cgaggatgtc ccatggagga cttgtaataa
     2701 gaaacggtat gacgggaagt gagaaaaacc aacatataat ggaattctca cttaaattag
     2761 acagcaatcc cgagtttacc ggaagcgtga ttctatcgta tgcaagagct gcttacagaa
     2821 tgaacaaaga aggaattaag ggagcaaaaa ccatttttga tgttcctctt tcatatatac
     2881 atcctgaagg cagagaaaaa ttaataagaa actttttata attgtttacg aaataaatac
     2941 attcgtatta aaaacgttga catttatatt tccttatgat attgtaaaaa agttataaat
     3001 taatttggag gaagaaatgt taaaggttgc taaatttgga ggtacttcat tagctgactc
     3061 taagcagttt aataaagtat atgacataat caaaaacaat gatgaaagaa aatatataat
     3121 agtatcagcc cccggcaaaa gattcaagga tgacaacaaa attaccgact tattgtatct
     3181 tacgtatgca catataaagt actcggtacc ctgtgcacct gttttaaaaa ttatagaaga
     3241 cagattcagg cagattaagg aagagctgaa cttgaaaacg gatttagact atgagtttaa
     3301 aattataaaa gaaaacttgg acaataaatg tgaagaagac tatatcgtaa gcaggggcga
     3361 atatttatgt gccttgttga tggctgatta cttggattgt gattttattg atgcaaagga
     3421 tgttattttt tttaactacg acggtacggt aaatgaggaa aaaactcatg aaaaactgac
     3481 agaaatactg ggcaaaagca ataaggcaat tataccgggc ttttacggct catatccgga
     3541 cggaagcata aagacttttt caagaggagg ctccgatatt accggctcga ttgtttcgag
     3601 tgttatggga gcagatatat atgaaaactg gacagacgta tcaggatttt tagttgcaga
     3661 ccctcgaatt gtaaacaatc ctaagcaaat taagaaaata acatatcagg
//
LOCUS       DAOSOW010000175         3688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_25116_length_3688_cov_2.825213, whole genome shotgun sequence.
ACCESSION   DAOSOW010000175 DAOSOW010000000
VERSION     DAOSOW010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25116_length_3688_cov_2.825213"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLD67_05395"
     CDS             complement(<1..208)
                     /locus_tag="PLD67_05395"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HOT21988.1"
                     /translation="MKYEKIIKALIHNGYKVSYFENSNEAAKYIVDNVKNTTIGFGDS
                     ATLESINLAELLSKNNTVIDPAPYS"
     regulatory      413..544
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            614..1246
                     /locus_tag="PLD67_05400"
     CDS             614..1246
                     /locus_tag="PLD67_05400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT21989.1"
                     /translation="MEVMSTLQDYDVSQFIQIPDLVKSRKATPKDLSDIYKVACSVGS
                     KSKESYAGFLMDDYKSRPKYYQKFFLERIFELDYFYVAESNIGIVGFLMAYTKEQWLK
                     YNPTWIEDIMWRPDFSMEKTSDFVIVDKTAIMANLTGKGIGSTLYKALLKDLKKKNIK
                     HIFAETLVDPVPNFASLAFRQKQKYTLAGTRYENYMDTLYTDLIYYKPVN"
     gene            complement(1284..1853)
                     /locus_tag="PLD67_05405"
     CDS             complement(1284..1853)
                     /locus_tag="PLD67_05405"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOT21990.1"
                     /translation="MYTIDELKKFCYRCHKCRLAETRNNVVFGEGNENADIMFVGEGP
                     GYYEDIQGRPFVGRAGQLLDKMIEAIGFKRNDVYIANVVKCRPPENRNPFDDECSACL
                     DYLRWQVKLIKPKIIVCLGAVSAKNLISPDLSISRQRGEFVIKGGIYFMPTYHPSYLL
                     RNESAKKDSWNDFKNIAAKIEEIRGTALD"
     gene            complement(1863..2204)
                     /locus_tag="PLD67_05410"
     CDS             complement(1863..2204)
                     /locus_tag="PLD67_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOT21991.1"
                     /translation="MNDNAPYKNNYDSCPLTFALTLIGGKWRLPVIWSIHKNKTMRYN
                     ELRRDIQGITSMMLTQSLKELEAYGIVERKQYAEIPPRVEYSLTDNGENLIPALKALA
                     DWGDKMRKLKA"
     gene            2334..2882
                     /locus_tag="PLD67_05415"
     CDS             2334..2882
                     /locus_tag="PLD67_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT21992.1"
                     /translation="MGLSIQYEIVSEDNIFIIKDLCNELMAYQKSKAYIHPEFFDNMS
                     FETRVLPAMKSSKENYIIAANDGDEVVGYAYSTIAPKTIYSGGFATLQCDAFFDFDSV
                     ETEDVGCLSQFFIKENYRNTGIGTTMFNMSMDWLNSFKEIKDIFIFVSNGNENALNFY
                     LNKGFKVSHEILDGFITVLRNK"
     gene            complement(2930..3181)
                     /locus_tag="PLD67_05420"
     CDS             complement(2930..3181)
                     /locus_tag="PLD67_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21993.1"
                     /translation="MSDKSKKSKDKYNIQWDINSMNASSSTELTGLIPVGPDSDEELA
                     SYDEILTYRAQNTVAESNEKSMNQTLHARKTPDFKNKKS"
     gene            3467..>3688
                     /locus_tag="PLD67_05425"
     CDS             3467..>3688
                     /locus_tag="PLD67_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT21994.1"
                     /translation="MGVETINSMLNKVNDNKIYDELKYQLRSYDEIANEAYGELVKRN
                     QEPKDISTVNKLSAKMSVGINTMISNTPSH"
BASE COUNT         1225 a          645 c          580 g         1238 t
ORIGIN      
        1 cggaatatgg tgccgggtct attactgtat tattttttga taaaagctca gctaaattta
       61 tagattcaag tgtagcggaa tcaccaaaac cgattgtagt attttttaca ttatcgacaa
      121 tatattttgc cgcttcattt gaattctcaa agtacgaaac cttatagccg ttatggatta
      181 atgctttaat tatcttttca tatttcatca aaccttcctc ctcccaaaca atcaaatatg
      241 ttcctgttaa attataccat ggcagaatat aaaggacaag aataaagaat aagttcgaca
      301 agatgcaaca cgtacaccac ttagaattgc aggtctacat gatttttcaa aatttattct
      361 ttacaataaa tcaagaatat ggtatacttc tatcaagtag attgtaatat tgtaaattgt
      421 aacgctgaat tttttgtata aaaaagcggg gaaaccaatt ttttggggtg aatctctatg
      481 agtagggtta acttctttcg acccgaaccc gtcagctaac ctcgtaagcg ttggaagagg
      541 aaaatgaaac aaataactgc atatatcatt ttcctatttt tgcaatcgct aattttaaag
      601 ggaggatgac aatatggaag ttatgagcac tttacaagat tatgatgtaa gtcaatttat
      661 tcaaattccg gacctagtta agtcaagaaa agcaacaccg aaagatttat cagacatcta
      721 taaagttgca tgctcggttg gttcaaaatc aaaagaatct tacgcaggtt tccttatgga
      781 tgactataaa tcgagaccaa aatattatca aaaattcttc ttggaaagaa tttttgaact
      841 ggattacttt tatgtagctg aaagcaatat cgggatagta ggttttttaa tggcctacac
      901 caaagaacaa tggttaaaat acaatcccac ttggattgaa gacattatgt ggcgaccgga
      961 cttctcgatg gaaaaaactt ctgactttgt gatagttgac aagacagcta taatggcaaa
     1021 tctaaccggt aaaggtatag gaagcacact ttataaagct ctcttaaaag atttgaaaaa
     1081 gaaaaatata aaacatatat ttgcagagac gcttgtagac ccggtaccta attttgcttc
     1141 tctggctttt agacaaaagc agaaatatac tttagccggc acacgttatg aaaattatat
     1201 ggatactcta tatactgact tgatttatta taaaccagta aactaatcct taatgactct
     1261 atcaagagtc attttttatt tgcttaatca agggctgttc cacgtatttc ctcgatttta
     1321 gcagcaatat ttttaaaatc attccatgaa tccttcttag ctgattcgtt tcttaacaga
     1381 tatgacggat gatatgtagg cataaaataa atgcctccct taattacaaa ctctcccctt
     1441 tgcctgctga tacttaaatc cgggcttatg agatttttag cggatactgc tccaagacat
     1501 acaataattt ttggtttaat caacttaacc tgccatctta aataatccag gcatgcactg
     1561 cattcatcat caaaaggatt tctgttttca ggaggcctgc attttacaac attggctata
     1621 tatacatcat ttcgtttaaa gcctattgct tcaatcattt tatcaagaag ctgtcctgct
     1681 ctgccgacaa aaggcctccc ttgaatatcc tcataatatc caggaccctc ccctacaaac
     1741 attatgtcag cattctcatt tccttcacca aatacaacat tatttcttgt ttcagctaat
     1801 ctgcacttat gacatctata gcaaaacttt tttagttcat caatcgtata cataatacct
     1861 cctcaggctt tcagcttcct cattttatct ccccaatcag ccaaggcctt aagagccggg
     1921 ataagatttt ctccgttatc agttaaggaa tattctaccc tgggcggtat ctcagcatac
     1981 tgctttctct cgacgatacc gtaagcttcc aattccttta gcgactgggt taacatcatg
     2041 cttgtaatac cctgaatatc tcttcttaac tcgttataac gcatagtttt atttttatga
     2101 atagaccaaa taacaggcaa tctccacttt ccgcctatga gagttaaggc aaatgtaagc
     2161 ggacagctgt cataattatt cttataaggt gcattgtcat tcatttaacc actccattac
     2221 tcagtttttt cttacttaga cataaaatat tgcatacttg ttacattttt tttagctgat
     2281 ataattataa tcgaagatta attaatcagt caaattttta ttacgaaagg atgatgggtt
     2341 tgagtataca gtatgaaatt gtttcagaag ataatatttt tattattaaa gatttatgca
     2401 atgagctaat ggcttatcaa aaatccaagg catatatcca tcctgaattt tttgacaata
     2461 tgagttttga aacaagggtg ctaccggcca tgaaaagctc aaaagaaaat tacataatag
     2521 cagctaatga cggcgatgaa gttgtcggat atgcttacag cacaattgca ccgaagacaa
     2581 tatactccgg cggctttgct actttacaat gtgatgcctt ttttgatttt gattctgtgg
     2641 aaacggaaga tgtaggatgc ctctctcaat tttttataaa ggaaaattat cgcaataccg
     2701 gcatcggtac aacaatgttc aacatgtcca tggattggct aaattcattt aaagaaataa
     2761 aagatatatt tatctttgta tcaaacggaa atgaaaatgc acttaatttt tatctgaata
     2821 aaggtttcaa ggtaagccat gaaattcttg acgggtttat aaccgtattg agaaataaat
     2881 aatatacaca aaaattccct tgaattttat tattcaaggg aattatttat taactctttt
     2941 tgtttttgaa atcaggtgtt tttcttgcat gcagtgtctg gttcatactt ttttcattgc
     3001 tctctgcaac tgtgttttga gctctatatg tcagaatttc atcataagat gctaattctt
     3061 cgtccgaatc aggtcctacc ggaattaatc ccgttaactc tgtagatgaa ctggcattca
     3121 tagaattaat atcccactga atattatatt tatcttttga ttttttactt ttgtcgctca
     3181 tgctgaatct ccttctgtat tttatttata gtatgtcatg aaaagagaaa ttttaaacaa
     3241 aatttccccc ttaaaatgag aatgctaagt aaagtatttt ataactgcat taaatgctca
     3301 aagttttatt ttatattatt gccttaaggc atcaatctat tatcatttac taagtttttg
     3361 tatcagaatt ttaatattgt aaataataaa ctaaaaagga ggtaaacaaa tgaataatga
     3421 tcaaaatact gtagaaattt tatccgaaat ttatcgcgga gctaaaatgg gcgttgaaac
     3481 aattaacagt atgctaaata aagttaatga caacaaaata tacgatgaac ttaagtatca
     3541 acttagaagt tatgatgaaa ttgctaatga agcgtatggc gaactggtaa aaagaaatca
     3601 agaacccaaa gatatttcca ccgtgaataa gcttagcgcc aaaatgagcg taggtattaa
     3661 tactatgatt agtaatacac catctcat
//
LOCUS       DAOSOW010000176         3669 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_25286_length_3669_cov_2.786110, whole genome shotgun sequence.
ACCESSION   DAOSOW010000176 DAOSOW010000000
VERSION     DAOSOW010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25286_length_3669_cov_2.786110"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLD67_05430"
     CDS             complement(<1..273)
                     /locus_tag="PLD67_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21995.1"
                     /translation="MLNLIKYEFIRKCRLVSMILITMLALNLFLLTRGVGGSMLFLVL
                     SPIVLYVFYLADIIKMYSDDLNKKTGYMLFMTPNSGYKIISSKLLAA"
     gene            complement(266..973)
                     /locus_tag="PLD67_05435"
     CDS             complement(266..973)
                     /locus_tag="PLD67_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT21996.1"
                     /translation="MNKLSVLKITDLTKRYYNKTALENVSLDFEQGKIYGLLGPNGSG
                     KTTLMKVVAGLHKQTSGSVLINDEMLSYKSKAYVSYMPTENYLYENFKVIDILKYYNT
                     MYEDFRYDYAVNLLNDIDVNSDIKISNLSSGQIGKLKTVIALSRDAQIYMLDEPLNGV
                     DVLSRDTILDLIAKSHSENKIIIISTHLVSEIEKILDTVIFLKEGIVEVFGDAEELRQ
                     ERQLSVEGIYKEVFRNA"
     gene            complement(966..1337)
                     /locus_tag="PLD67_05440"
     CDS             complement(966..1337)
                     /locus_tag="PLD67_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017754346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOT21997.1"
                     /translation="MEFNNNIPIYLQVIEKIKQDIVSGKLKPGEKMPSSRDYSNELGI
                     NFNTVARVYRELENEEIVFTKRGLGTFITESDQIIEKLRYTMAKNQITAFIDGMKQLG
                     YTKEDMIKFIETENNYLEGNE"
     gene            1541..2614
                     /locus_tag="PLD67_05445"
     CDS             1541..2614
                     /locus_tag="PLD67_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005210260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOT21998.1"
                     /translation="MHKKLFIPGPVEVKEDVLSKMATPLIGHRSEDASNLQRDISNNL
                     KKLFYTENEILLSTSSGTGLMEAAVRCCTKERAAMFSIGSFGDRWHEMAVSNGINADL
                     FKSELGSITRPEMVDDVLKTGKYDLVTITHNETSTGVMNPIDKISNVIKKYPDVIFCV
                     DAVSSAGGVKIETDKLGIDICITSSQKALGLPPGLSMCTISERAVEKARYIKNRGYYL
                     DLVQLYDTIKKKDYQYPSTPNLSLMFALNYQLKLIMQEGLDNRFNRHFAMAEYTRSWA
                     KKNFALFADEKYASNTLTTVKNIRNISVKDLNIELGKRGFAISNGYGSLKEKTFRIAH
                     MADLILEDVKELILNIEDILSIS"
     gene            2638..3417
                     /locus_tag="PLD67_05450"
     CDS             2638..3417
                     /locus_tag="PLD67_05450"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOT21999.1"
                     /translation="MYDLNKISSYLNTNTIGQSIIQYDNLNSTFAKAKNIFSTCPDGT
                     VVLSENQSECILRFGNEWLCSPDKNIYLSIILKSINNNYLVPLTDVVACSSILKSIEE
                     LYNLDCRIKWPNDIFINNNKISSVKSEIIGKGAGIILSLYINVNMEEKIENLKATSIK
                     IEINNDVERELLIGTILNNIEYHYVEMINTGKAYHSVDIYNENLLLNNKVVGVIKRGR
                     KTVRKAVAKNIDSDGWLQVINEKGEEEILDPGETIIQYEET"
     gene            3404..>3669
                     /locus_tag="PLD67_05455"
     CDS             3404..>3669
                     /locus_tag="PLD67_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HOT22000.1"
                     /translation="MKKLKIGSIELDTNVVLAPMAGITDITYRTICKEMGAGLVHTEM
                     ISAKGLYYQDRKTEELMKINKNNRPVSMQIFGKSAIMKMRLVSM"
BASE COUNT         1302 a          560 c          614 g         1193 t
ORIGIN      
        1 agctgctaag agtttcgatg atatgatttt ataaccgctg ttaggtgtca taaacagcat
       61 gtaacctgtt ttcttattaa ggtcatcgct gtacattttt ataatgtctg ccaagtagaa
      121 aacatataaa actatgggag ataacacaag aaataacatg cttccgccta ctcctcttgt
      181 cagtaagaac aaattcaatg ctagcattgt aatcagtatc atagaaacca atctacactt
      241 cctaataaac tcatacttaa ttaaattaag catttctgaa tacctcctta tatattcctt
      301 caaccgacaa ttgcctttcc tgccttaatt cttcagcatc gccgaaaacc tctacaatac
      361 cttcttttaa gaaaataacg gtatcaagta ttttttcaat ttcactgact aaatgtgttg
      421 aaataataat tattttattt tcactgtgag attttgcaat taaatccaaa atggtatccc
      481 ttgataaaac atctacaccg ttcaaaggtt cgtcaagcat gtatatttgt gcatctctcg
      541 ataatgcaat aaccgtctta agctttccga tttgacccga tgacaaattt gaaattttaa
      601 tgtctgaatt tacgtctata tcatttaaaa gattaacggc ataatcatac ctgaaatctt
      661 catacattgt attgtagtat tttaatatat caatgacttt aaaattttca tacaaatagt
      721 tttctgtagg catgtatgaa acatatgcct tggacttgta ggataacatt tcgtcattta
      781 ttaatacact gccgcttgtt tgcttatgaa gacctgccac aactttcata agagtggttt
      841 tgccgcttcc gtttggccca agtaagccgt aaattttacc ttgctcaaaa tccaagctga
      901 cattttccag tgctgtttta ttgtaatatc tttttgttaa atcagtaatt tttaacacac
      961 tcaatttatt cattcccttc taaataatta ttttctgttt cgataaattt aatcatatct
     1021 tccttggtgt atcctaattg tttcattcca tcaataaatg cagtgatttg atttttagcc
     1081 attgtgtatc ttaatttttc aattatttgg tcagattctg ttatgaatgt tcccaaacct
     1141 ctttttgtaa atactatttc ctcattttcc aactccctat atactcgtgc aaccgtattg
     1201 aaatttatac ctaattcatt ggagtagtct cttgaagaag gcattttttc tccgggcttt
     1261 agtttccctg aaactatatc ttgtttaatc ttttctatta cttgtagata tataggtata
     1321 ttgttattga attccaactt atcaccttct tttgtatatt agtgtcatag tagaatagta
     1381 tactaatact tgagttatgt caatagttta atgttaacat ttcaaacaat aattagttac
     1441 aatttcgtaa caattgcttt taattataac ttgacataaa attgataaag ttatataaat
     1501 ggatagagaa attgagttat tacatagagc ggagggaaca atgcataaaa aattatttat
     1561 tcctggaccc gtagaagtaa aggaagatgt gctcagtaaa atggctactc cactgattgg
     1621 tcataggagc gaggatgctt ctaatctaca aagagatatt tcaaataatc ttaaaaaatt
     1681 attttataca gaaaatgaaa tattattgtc aacatcttca ggtacaggat tgatggaggc
     1741 agcggtgcga tgctgcacca aagagagggc tgctatgttt tcaataggct ccttcggcga
     1801 cagatggcat gaaatggctg tttcaaacgg tatcaatgcc gatttattca aatcagaatt
     1861 aggaagtatt acaagacctg aaatggttga tgatgtatta aaaacaggaa agtatgattt
     1921 agttacaata actcacaatg aaacatcaac gggagtaatg aatcctattg ataaaataag
     1981 taatgtaata aaaaaatatc cggatgtaat tttctgtgtt gatgcagtaa gctctgcagg
     2041 aggagtaaaa attgagacag ataaacttgg aatagatatc tgcataacat catcacaaaa
     2101 agcattgggg ctgcccccgg gactctctat gtgcaccata tccgagaggg ctgtggaaaa
     2161 agcaagatat ataaaaaaca gggggtatta cttagatttg gttcaattgt atgatacaat
     2221 caaaaaaaag gattatcagt atccgtcgac accaaattta tcattgatgt ttgctttgaa
     2281 ttatcaatta aaactgataa tgcaggaggg gcttgataac aggtttaaca gacatttcgc
     2341 catggctgaa tacacaagaa gctgggcaaa aaagaatttt gcattattcg cggatgaaaa
     2401 atatgcttcc aatacgctta ccacagtgaa aaacattcga aatataagcg taaaggattt
     2461 aaacattgaa ttagggaaaa gaggttttgc tatttctaac ggctatggca gtttaaaaga
     2521 aaaaacattc agaattgcac acatggcgga cttaatttta gaagatgtaa aagaacttat
     2581 tttaaatata gaagatattt tatcaatttc ttaaacatat aatatcgggg gatttaaatg
     2641 tacgatttaa acaaaatcag cagttattta aatacaaata caattggcca gtctattatt
     2701 caatacgaca atttgaattc tacgtttgca aaagccaaaa atatattttc aacatgtcct
     2761 gacggaactg ttgtattatc ggaaaaccaa tccgaatgta ttttaaggtt tggcaacgaa
     2821 tggctttgct cacctgataa aaatatttat ttaagtatta ttttaaaatc tattaataat
     2881 aattatctgg tacccttaac agatgtggtt gcttgttctt ctatattgaa atccatagag
     2941 gagctgtaca acttggattg cagaataaaa tggcctaatg atatttttat aaataataac
     3001 aaaatatcgt ctgtaaaaag tgaaattata ggcaagggtg caggtataat attatcctta
     3061 tatataaatg ttaatatgga agaaaaaatt gaaaatttaa aagcgacatc aataaaaatc
     3121 gaaattaata atgatgttga aagagaactt cttataggca caatattaaa caacattgaa
     3181 tatcattatg ttgaaatgat aaacaccggt aaggcttatc attccgttga catatataat
     3241 gaaaacttgc tgcttaataa taaggtagtc ggagttatca aaaggggcag aaaaacggtt
     3301 agaaaagctg ttgctaagaa tattgacagc gatggatggc ttcaagtaat caatgagaaa
     3361 ggcgaagaag aaatattaga tccgggagaa actataatac aatatgaaga aacttaaaat
     3421 tggaagtatt gaattagata caaatgttgt gcttgcaccc atggcaggca taacagatat
     3481 aacatataga actatttgca aagaaatggg agcgggtctt gttcataccg aaatgataag
     3541 tgcaaaagga ttgtactatc aagacagaaa aacagaagag cttatgaaaa taaacaaaaa
     3601 caacagacct gtgtcaatgc agatttttgg aaagagtgct atcatgaaga tgagattagt
     3661 ctcgatgaa
//
LOCUS       DAOSOW010000177         3649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_25455_length_3649_cov_3.479410, whole genome shotgun sequence.
ACCESSION   DAOSOW010000177 DAOSOW010000000
VERSION     DAOSOW010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25455_length_3649_cov_3.479410"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1188
                     /locus_tag="PLD67_05460"
     CDS             247..1188
                     /locus_tag="PLD67_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT22001.1"
                     /translation="MIKHFFKVFFASFLLIAIILSVTALGYIFLVDKDIVIGKIIFAN
                     DEKSEAAFYESSSFDYGTSIGKAASNMKRFNILLAGLENQRTDTIIALSYDMESKTAD
                     LISLPRDTYLPRNADDNAELKKINAIYSHEGIEGLALRVQELLGIPIEKYVVIDYEGV
                     IACVDMIGGVEVNVPFHMEYTDPYDDPPLYIDIPEGVQLLNGEQSLKFLRYRKGYDNQ
                     DLGRIEAQQQFIKSAVKKTLGFQLPAVIKEAYSHIETNINVSDLLYLAGDLVGFSTDK
                     INTHILPGMETPLEGLSFYIPDEEGIKNLVYSLYGLN"
     gene            1224..2231
                     /locus_tag="PLD67_05465"
     CDS             1224..2231
                     /locus_tag="PLD67_05465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HOT22002.1"
                     /translation="MKQHFIKVFLISLIIFSTVITTGIFTYAKFMSPEGITQKLPEDE
                     EPIAIDEEDENLTPLEKAMRDSNRINALLIGFEGARSDTIMLGSFDRKTKEANIISIP
                     RDTYYKRDAYAKYSEMQKINAVYGSEDDGYKALLKAVEEITGISVDKYVAVNYNGVRA
                     AVDAIGGVEVNVPIHMKYTDIYDKPPLYIDIKPGPQIIYGDKAMELLRFRTGDPGYPS
                     FPNGDVGRVEMQQQFIKSAIKKSLSLRLPNVISAVYPHVETNFTLTELLGLAKDAIGF
                     STDNLTTQILPGNPKILGSNPPLSFYIPDGQEITKVVYDLYDVPLSASAEEENVAHAS
                     E"
     gene            2249..3217
                     /locus_tag="PLD67_05470"
     CDS             2249..3217
                     /locus_tag="PLD67_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOT22003.1"
                     /translation="MIKPKPLKTGDKVAVIAPSSATDLKSVKNGERKIRALGLEPVMF
                     PTCYTNYGHLSASDEERAKDVNDAFKDETIKGIICLRGGYGATRILDLLDYETIASNP
                     KIFVGFSDITALHTAFNQICNMVTFHGPMAATRFANVKNDKVEFDVYSCESLVKNIFT
                     DIPIGLHKNPNNEELKSLNTGKAKGKLAGGNLTLLTATLGSKYEIDTKGKILFIEEIG
                     EPVYKLDRMLTTLALAGKFNECAGIILGTFIKCEREKKPYEGGLDLTLEEVVENTLVK
                     YNKPIIYNFKAGHNFPQPTIALGTTVYIDAELKQVEFLESGTSANQ"
     gene            3320..>3649
                     /locus_tag="PLD67_05475"
     CDS             3320..>3649
                     /locus_tag="PLD67_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOT22004.1"
                     /translation="MDIYKRIDEIFDELVQIRRDFHMHPELSQYEFRTQRKIREYLNK
                     WGIENYTCAETGVVGIIRGKNKGKTVGIRGDIDALPIHEENDVPYRSLTPGVMHACGH
                     DAHAAIVL"
BASE COUNT         1300 a          566 c          748 g         1035 t
ORIGIN      
        1 aatcgaacca ttttaccgta cctgttttca tttaaaaaaa tcctccgata ttaaaaaaga
       61 tatcattatt gtaacagttt tttttgtaaa ttgcaacata tttatttctt aaataaaaaa
      121 atcattctta atgtttgtta ttaagttggt gcaaattgtg cgcggcaaaa taatttttac
      181 aatccaattg aaaaagaaac ggtactttgt tataataatt tagcgatgaa tttataaagg
      241 tgggacatga ttaaacattt ttttaaggta ttttttgcgt cttttttact tattgccata
      301 atattgagtg ttactgcatt agggtatatt tttctggtcg ataaggatat tgtgataggg
      361 aaaatcatat ttgcaaatga tgagaagagc gaggctgctt tctatgaatc gagttccttt
      421 gattatggaa cttcgatagg aaaagctgcg tctaatatga aaagattcaa tattcttttg
      481 gcaggactgg aaaatcaaag aacagataca ataatagctc taagttacga tatggagagt
      541 aaaacagctg acttgatttc attgccaaga gatacatatc ttccaagaaa tgcagatgat
      601 aatgccgaat taaaaaaaat taatgcaata tattctcatg aagggattga aggacttgcg
      661 ttaagagttc aggaactgtt gggaattccg attgaaaagt atgtggttat tgattatgag
      721 ggagtaattg catgcgttga tatgattgga ggagttgagg tgaatgtccc ctttcatatg
      781 gaatataccg acccctacga tgatcctccc ttatatatag atattcctga aggtgtgcag
      841 cttcttaacg gggagcagtc tttaaaattt ttaagataca gaaagggata tgacaaccag
      901 gatttgggga gaatagaagc acaacagcaa tttattaaat ctgccgttaa aaaaactttg
      961 ggttttcaac tgcccgctgt gataaaagaa gcttattctc acattgaaac aaacattaat
     1021 gtttcggatt tgctgtactt ggcaggcgat ttggtcggat tttccaccga taaaattaat
     1081 acacatatat tgcccggcat ggaaacaccc ttggaaggat tatcctttta cataccggat
     1141 gaagaaggta taaagaattt agtttattca ctatacggat taaattaaaa catacttgca
     1201 taataccgaa aggcggtttt tttatgaaac aacattttat taaagtattt ctcatatcct
     1261 taataatatt ttcgactgtg ataaccacag gtatttttac gtatgcaaaa ttcatgtctc
     1321 ctgaaggaat aactcaaaaa ctgccggagg atgaagaacc cattgcaatc gatgaagaag
     1381 atgaaaattt aacacccttg gaaaaagcaa tgcgtgacag taatcgcata aatgctttgc
     1441 tgataggatt tgaaggagca aggtcagaca cgataatgct tggaagcttt gacaggaaaa
     1501 ccaaagaagc aaacataata tcgataccga gagatactta ttataaaaga gatgcatatg
     1561 caaaatattc tgaaatgcaa aaaataaatg ctgtctacgg cagcgaagat gatggatata
     1621 aagctttatt gaaagctgtg gaagaaataa caggcatttc cgtagacaaa tatgttgccg
     1681 ttaattataa cggtgtaaga gcagctgtag atgctatcgg cggtgttgaa gtaaatgtac
     1741 ccatacatat gaaatatacg gatatatatg acaaaccgcc tctatatata gatataaaac
     1801 cgggaccgca aattatatac ggcgataagg caatggaact tctgaggttc agaacagggg
     1861 acccgggcta tccttctttc ccgaacggag atgtgggaag agtggaaatg cagcagcaat
     1921 ttattaaatc agcaattaaa aaatcactga gcctaaggct tccaaatgta ataagcgctg
     1981 tttatcctca tgtcgaaaca aatttcactt taacggaatt attggggctt gcaaaggatg
     2041 caatcggatt ttcaacagac aaccttacga cacaaatttt gccgggcaat ccaaaaatat
     2101 taggcagtaa tccgccctta tctttctata taccggacgg tcaggaaata accaaggttg
     2161 tatacgattt atatgatgta ccgctgtctg caagcgcaga ggaagaaaat gtagctcatg
     2221 cttctgagta acacaggggg gtatttaaat gataaaacca aaaccattaa aaaccggtga
     2281 taaagttgca gtaatagctc cttcctcagc gactgatttg aaatccgtaa aaaacggaga
     2341 aaggaaaatt agagccttag gattagagcc tgtaatgttt ccaacctgtt acacaaatta
     2401 cggacacctt tctgcatcag atgaagaaag ggcgaaagat gttaatgatg cctttaaaga
     2461 tgaaactatt aaaggcataa tttgtttaag aggaggttat ggagctactc gaatactgga
     2521 tttgttagat tacgaaacga tagcatcaaa ccccaagatt tttgtaggat tcagcgacat
     2581 tacagctctc catacagctt ttaatcaaat atgcaatatg gttacttttc atggtcctat
     2641 ggctgctaca agatttgcaa acgttaaaaa tgataaagtt gaatttgatg tttattcctg
     2701 tgaaagcttg gtaaaaaata tttttaccga tattcccata ggtttgcaca aaaatccaaa
     2761 caatgaagaa ttaaaaagtt taaatacagg caaggctaag ggaaaattag ccggagggaa
     2821 tctgacgctt ttaactgcta ccttaggctc aaaatatgaa attgacacaa agggaaaaat
     2881 acttttcatt gaagaaatag gagagcctgt ttacaaattg gacagaatgc ttacaacact
     2941 ggcattggca ggaaagttta atgagtgtgc aggaataata ctgggaacct ttataaaatg
     3001 cgaaagagaa aagaaaccct acgaaggagg gttggacctt actctggaag aagttgtgga
     3061 aaacacctta gttaaatata acaaacctat aatttataat tttaaggcag gacataattt
     3121 tcctcagcct acaatagctt tagggacaac tgtttacata gatgcagaat tgaaacaagt
     3181 agaatttttg gaaagcggaa catcagcaaa tcagtaatat aagagtatgg agacacacct
     3241 ccctatataa ggaaaagtct ctgccggtag gaggaatgtc cccgaatagt atattttaaa
     3301 aagaaattaa ggagtcaata tggatattta taaaagaatt gatgaaatat ttgatgaatt
     3361 ggtgcaaata cgcagggact ttcatatgca tccggaactt agccaatatg agttcagaac
     3421 acagaggaaa attcgtgaat acttgaataa gtggggaata gaaaattata cttgtgcaga
     3481 aacaggtgta gtagggataa taagaggaaa aaacaaggga aaaaccgttg gaataagagg
     3541 agatattgat gcactgccca tacatgaaga aaatgacgtg ccatatcgct ctttaactcc
     3601 cggtgtgatg catgcatgcg gtcacgacgc tcatgcggca atagtgctt
//
LOCUS       DAOSOW010000178         3642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_25525_length_3642_cov_3.819905, whole genome shotgun sequence.
ACCESSION   DAOSOW010000178 DAOSOW010000000
VERSION     DAOSOW010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25525_length_3642_cov_3.819905"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="PLD67_05480"
     CDS             <1..596
                     /locus_tag="PLD67_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22005.1"
                     /translation="AGGDATALVGGTAIIIMLLTVLTSFKLRDGFELIVNYIKEGFKF
                     GISVFAPVLFIGGFFFLGNQNGAAAVLGEGAPGILNDISLFLSENIQMTKLSTILTQT
                     AVALITGLDGSGFSGLPIVGTLAYTFSNVIDIDMAGLAALGQVITVWVDGGTIIPWAV
                     IPVAAICNISPQELARKNLIPVLCGIGAAIAVALVIL"
     gene            complement(726..3554)
                     /gene="uvrA"
                     /locus_tag="PLD67_05485"
     CDS             complement(726..3554)
                     /gene="uvrA"
                     /locus_tag="PLD67_05485"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOT22006.1"
                     /translation="MDKIIIKGAKENNLKNISIELPREKFIVFTGVSGSGKTSLAFDT
                     IYAEGQRRYVESLSSYARMFLGQMDKPDVESIEGLSPAISIDQKSTSRNPRSTVGTIT
                     EIYDYYRLLFARIGVPHCPNCGKPITTQTVDQIIDKVLEYEEGTRIQVLAPMVRRAKG
                     THKKLLEDIKKEGFVRVRVNGELFELEEDIILDKNKKHTIEVVIDRLIIKAGIQKRLV
                     DSVETALTLSGGMVLIDVMGQEELLMSTKFACTECGIGFGEISPRMFSFNNPIGACET
                     CNGLGVKREIDVDLVIPDYSKTIGERAIAPFGAEEDGYYYQMFAALAKYHNFDISKPL
                     SQAPEEFIDELLYGTNGREVKVNFISKFQGAREYVREFEGVVNNLQRRYNETRSPDMR
                     QWIEEYMYESPCPACKGARLNPISLAVKVGNKNIYEVTQLSVVKSLEFFDKLDLSPMN
                     RIIGGQIIKEIRDRLQFLSDVGLNYLTLARNASTLSGGESQRIRLATQIGSSLVGVLY
                     VLDEPSIGLHQRDNAMLIKTLRSLTDLGNTLIVVEHDEETMQNADYILDIGPGAGVHG
                     GEVVGFGTIDEIKNNPNSITGQYLSGKKKIEVPAQRRKAEKFIEIKGAKENNLKNIDV
                     KIPLGIMCCVTGVSGSGKSSLINEILAKGLHQKLFKNTNRPGKFDQMLGIENIDKIIT
                     IDQSPIGRTPRSNPATYTGVFDYIRDVYSMTLESKERGYSKGRFSFNVKGGRCEACRG
                     DGILKIEMHFLPDVYVPCDVCKGKRYNRETLEVKYKGKNIADVLDMTVDEALDFFENI
                     PRIQNKIKTLQDVGLGYVKLGQPSPELSGGEAQRVKLAYELSKRGTGKTVYVLDEPTT
                     GLHIADIHMLIDVLNRLVEAGNTVIVIEHNLDVIKTADYIIDLGPEGGDGGGTIVAQG
                     TPEEVAEVKNSYTGQYLKKVLWDSGDVS"
BASE COUNT          973 a          793 c          598 g         1278 t
ORIGIN      
        1 tggcaggagg cgatgcaacc gcacttgtag gaggaaccgc aataatcata atgctgttaa
       61 ctgttttaac aagctttaaa ttaagagatg gttttgaatt aatagtaaat tatataaaag
      121 agggttttaa atttggaatt tctgtttttg ctcccgtact tttcatagga ggatttttct
      181 tcttaggtaa tcagaatgga gctgcagcag tattgggtga aggagcaccc ggtatattga
      241 atgacataag tttattctta tctgaaaaca tacaaatgac caaactttcg actattttaa
      301 ctcagactgc cgtagccctc ataacaggtt tggacggttc aggattttca ggacttccaa
      361 ttgtaggtac cttggcttac acattttcaa atgttatcga tattgatatg gcaggattgg
      421 ctgccttagg tcaagtaata acagtatggg ttgacggagg aaccataatt ccttgggctg
      481 ttattcctgt ggctgcgatt tgtaatataa gtcctcagga gctggcaaga aaaaatttaa
      541 taccggtgct ttgcggaatc ggtgctgcaa ttgcagtagc attagtaata ctatagaaag
      601 acattgggaa cgtatcttaa ctgtcccact taacaaatat ggctaagcct actgtcatat
      661 atgacaataa tccttagccg tttttattta ataattgttt ttaatattcc atagaaatgg
      721 taaagttatg atacgtcccc actgtcccat aaaacctttt ttaagtattg ccctgtataa
      781 gagttcttta cttcagccac ttcctcagga gtaccttgtg ctacgattgt tcctccgccg
      841 tcaccgcctt cgggacctaa atctataata taatctgcag tttttataac atctaaatta
      901 tgctcgatta caattaccgt attgcctgct tccacaagtc tgttcaaaac atctataagc
      961 atatgaatat ccgctatgtg aagaccggtt gtaggttcat ccaagacgta gactgtttta
     1021 cccgtacctc ttttactcag ctcatatgcc agcttaacac gctgtgcttc tcctccggat
     1081 aattcaggtg aaggctgacc taatttcaca tatcccagtc caacatcctg caatgttttt
     1141 attttatttt gaattctggg aatattttca aagaaatcta aggcttcatc aacggtcata
     1201 tctaatacat ccgctatatt tttgccctta tattttactt ccaatgtttc tctattgtat
     1261 cttttaccct tgcacacatc gcagggcaca tatacatcag gcagaaagtg catttcaatt
     1321 tttaaaattc catccccgcg gcatgcttcg catctcccgc ctttaacatt aaaactgaat
     1381 ctccctttac tatagcctct ttccttagat tcaagagtca tcgaataaac atccctgatg
     1441 tagtcaaata ctcccgtgta tgtggcaggg ttagacctgg gggtccttcc tatcggtgac
     1501 tggtcaatgg taattatttt atctatattt tcgatgccaa gcatttggtc gaattttccg
     1561 ggacgatttg tgttcttgaa taatttctgg tgaagaccct ttgccaatat ttcatttatc
     1621 aaggagcttt taccggagcc ggatacccct gtaacgcagc acattatccc taatggtatc
     1681 ttcacgtcta tattcttaag gttattttct ttggcaccct taatttcaat aaatttttca
     1741 gcctttcttc tttgagcagg cacttcgatt tttttctttc cgctcaaata ttgtccggtt
     1801 attgaatttg gattgttttt aatttcatct attgtcccaa atcctactac ttccccgccg
     1861 tgcactcctg ctccgggacc tatatccaaa atataatctg cattttgcat agtttcttca
     1921 tcatgctcaa ccactataag cgtattacct aaatctgtta aacttcgcaa tgttttaata
     1981 agcattgcat tatctctttg atggagtcct atgcttggct catccaatac atataatacc
     2041 cccacaagac ttgaacctat ttgagtggcc agtctgattc gctgagactc gcctcctgac
     2101 aatgtagatg catttcttgc caatgtgaga taattaagac caacatctga caaaaattgc
     2161 aatctgtccc gaatttcttt gattatctgt ccgccgatta ttctattcat gggactcaaa
     2221 tccagcttat caaaaaactc caggcttttt acaactgaga gctgagttac ttcatatatg
     2281 tttttgttac ctacttttac tgcaaggctt ataggattta accttgctcc cttgcatgca
     2341 ggacaaggac tttcatacat atattcctct atccattgtc tcatgtcagg agatctggtt
     2401 tcgttatatc ttctttgcag attgtttaca actccttcaa attctctgac atattctctt
     2461 gctccttgaa atttgctgat aaaatttact tttacttctc ttccgtttgt accgtacaaa
     2521 agttcatcga tgaactcttc tggagcttgc gaaagaggtt tggatatatc aaaattatgg
     2581 tatttcgcca gcgctgcaaa catttgataa tagtatccgt cttcttccgc accaaaaggt
     2641 gctattgctc tttcgcctat tgtctttgaa taatcgggaa ttaccaagtc aacatctatt
     2701 tctcttttaa caccaagacc gttgcatgtt tcacatgctc ctatgggatt attgaaagaa
     2761 aacatcctcg gggaaatttc cccaaaacct atgccgcatt ccgtgcatgc aaattttgtg
     2821 ctcattaata gttcttcctg acccattacg tcaatcagaa ccatccctcc ggataatgtc
     2881 aaggccgttt ccaccgaatc cacaagcctt ttttgtattc cggctttaat aataagcctg
     2941 tctataacaa cttcaatagt atgtttttta tttttatcca atattatatc ttcttctaat
     3001 tcaaaaagtt ctccgttaac cctcactctg acaaagcctt ctttttttat gtcctccaat
     3061 aattttttat gagttccctt tgcacgtctc accattggag ctaaaacttg aattcttgtt
     3121 ccttcttcat attccaatac tttgtcaata atctggtcta ctgtctgagt tgtaatcggt
     3181 tttccgcagt ttggacagtg aggtacacct attcttgcaa acaaaagtct gtaataatca
     3241 taaatttcag taatcgtacc cacagttgaa cgcgggttcc tgctggttga tttttggtca
     3301 attgaaattg cgggtgacaa tccctcaatc gattccacat caggtttatc catctgtccc
     3361 aaaaacattc ttgcatagga ggacaaactt tcaacataac gtctttgtcc ttctgcatat
     3421 attgtatcaa aagcaaggga agtcttgcct gagccgctca ctcccgtaaa aactataaat
     3481 ttttctcttg gaagttcaat gctgatattt ttcagattgt tttcctttgc tcctttaata
     3541 attattttgt ccattattta tcctctctta ggtatgggga cacatcatat catatagtct
     3601 atgcagatca aagtaatgtc ccaattttat gtcattctta tg
//
LOCUS       DAOSOW010000179         3640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_25540_length_3640_cov_4.046304, whole genome shotgun sequence.
ACCESSION   DAOSOW010000179 DAOSOW010000000
VERSION     DAOSOW010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25540_length_3640_cov_4.046304"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PLD67_05490"
     CDS             complement(<1..599)
                     /locus_tag="PLD67_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012058141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT22007.1"
                     /translation="MKYDLIIVGAGPSGIFTALELNRENPNKKIMIVEQGKAIEKRNC
                     PKDITKKCISCQPYCNITTGFAGAGAFSDGKLSLSPEVGGDLPELIGYDFTQQLIEYT
                     DSIYLEFGADSRVEGVEAKEEIKEIRRKAIEAGLKLVDCPIRHLGTERAQEIYKTIEK
                     HIIDKGVEIKFNTEAKDILVENGRIKGVVVTDSSKKNSS"
     gene            complement(647..1822)
                     /locus_tag="PLD67_05495"
     CDS             complement(647..1822)
                     /locus_tag="PLD67_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOT22008.1"
                     /translation="MKKKKYFIATGVIVIIFIIASLLLGGQTDQPETSKVRPGSIKKT
                     VEETGTVFSKRVNTFYSDMSQRVETLNVSVGDRVKKGDIILVYENNYDLEIERANKQI
                     EAITASYNESVKGADFQEISNLKLNISTIETNLNFARSNLEKIKTLYENNAISQVEYD
                     EAKNNVAILENQLQEANNNYSKIIQGVSANVKKEYEAQIDEVMIQIKILEKNMEQASV
                     KAEFDGVITELNVHQGSMTQAGVPTVEIQDDKNLGIYVEVLAEDAIEVTKGMAFNIID
                     DDEVIKELQVSRVYPKAQAKISDLGVEQKRVRIEADLDDADYKLGSEVDVEIVLKEKN
                     NVLLVDKDAVYEKDNIKYVTVLSGKNEIETEITTGIEDDKYVEVLSGLSENDTVLIK"
     gene            complement(1836..>3640)
                     /locus_tag="PLD67_05500"
     CDS             complement(1836..>3640)
                     /locus_tag="PLD67_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT22009.1"
                     /translation="QTILPDPENFGVVFLEENYLRKIHGINGNFNEILIKVKDENYIE
                     QIVDFLEDNLDKYGVTRIIKREDQLSNNLLSQEISGLEMMSKSIPVVFLSFAGVMLAV
                     MLSRIVKKDRTSIGVLKALGFTDKEIVIHYLKYAAIVGLIGGFLGSIIGTALSGVMTN
                     YYLVFFNIPMLTVKVYYYRIIISVILSLFFCTASGFLGVREIIKINPAESMKPEAPKE
                     GKRIIIENIKIMWSHVPFTWKIVLRNIFREKKKFIFIGAAVAITCGMMIMTTWMIDII
                     DVMFNRHYSEFMKAQYNISFNGFTNENVLNEFSEQLSIDKMEGRTELPFEIENGRKSK
                     IVTIIGLRENTDFYGFKNIDGNKIKLPSNGILLSSNLAKSLNVIVGDRILIKSLISDK
                     DVYVTVKGIVNQVLGINAYMNIDYLNNMFLDKGIINGVYLNSKDNVKGKLDDMKKITI
                     QSQDDMIKAFEEFTTVTAVSMGIMVVFSGILGFVIVYSMTLMSINERTLEFSSLRVMG
                     FSKKEIFNMLVRENTIMTFIGIVAGIPVGLWLVNYMGETFTTDIYTMAEPVNPISIFV
                     SIILTVIFVISAQLMTYAKIHKLDFMQALKSRIT"
BASE COUNT         1066 a          779 c          447 g         1348 t
ORIGIN      
        1 tcactgctat ttttctttga cgagtcggtg accacaacac ctttaatcct gccattttca
       61 actaatatat ctttagcctc agtgttgaat ttaatttcaa cgcctttgtc aattatatgt
      121 ttttcaatag ttttgtagat ttcttgagcc ctttccgtcc ccaaatgtct tatgggacaa
      181 tccacaagct tgagtccggc ttcaatagct tttcttctta tctccttaat ttcttccttg
      241 gcttcaacac cttctacccg gctatcagca ccaaattcaa gataaatcga atctgtgtat
      301 tcaattaact gctgtgtaaa atcatatcct attaattcag gcaagtcacc accaacctcc
      361 gggcttaaag acagcttgcc gtcagaaaat gcacctgctc ccgcaaagcc cgttgtaata
      421 ttgcaataag gttgacagct gatacatttt tttgttatat ccttcggaca atttctcttt
      481 tcaattgcct ttccctgttc aactatcata atttttttat tagggttttc cctgtttaac
      541 tccaaggctg taaatatccc tgacgggcct gcccctacta taatcaaatc atatttcata
      601 atctttctcc ctccaacgag tacattcgaa agtgtgttac attcctttat tttattaaga
      661 ctgtgtcatt ctcgcttaat cccgatagta cttccacata tttgtcatcc tcaattccgg
      721 ttgttatttc ggtttcaatt tcatttttcc cgcttaaaac agtcacgtat tttatattat
      781 ccttttcata aacagcgtcc ttatctacca gtagtacatt attcttctcc ttaagtacta
      841 tttctacatc aacctccgag cccagcttat agtctgcatc atccaagtcc gcctcaattc
      901 tcacacgttt ttgctcaacg ccaaggtctg aaatttttgc ctgagctttc gggtaaaccc
      961 tgcttacctg taattctttt ataacttcat catcatctat aatattaaaa gccatgccct
     1021 ttgtaacttc tattgcatct tcagctaaca cttccacata aatgccgagg tttttgtcat
     1081 cctgtatttc gactgtcggg acccctgcct gtgtcatgct gccctgatga acatttaatt
     1141 ctgtaatcac accgtcaaat tcagctttta cggaagcttg ctccatattc ttttccagta
     1201 ttttaatttg tatcatcact tcatctattt gagcttcata ttcttttttt acatttgctg
     1261 aaactccctg tattatctta ctgtagttgt tatttgcttc ctgaagctgg ttttccagta
     1321 ttgcaacatt attttttgct tcatcgtatt ctacttggct aatggcatta ttttcatata
     1381 aagttttaat tttttcaaga ttgcttcttg caaaattaag attagtttca attgtgctta
     1441 tgttcagttt taaatttgaa atttcctgaa agtccgcccc ctttacggat tcattgtagg
     1501 atgccgtaat tgcttcaatt tgcttgtttg ctctttcaat ttccaaatca taattgtttt
     1561 cataaactaa aattatgtca ccctttttta ccctgtctcc tacagacaca tttagagttt
     1621 caactctttg gctcatatcc gaataaaagg tattaactct tttagaaaac actgttcctg
     1681 tttcttctac cgttttttta atactacccg gtctaacctt agatgtttca ggttggtctg
     1741 tctgccctcc caacaataat gaagctatta tgaaaataat tactattact cctgttgcaa
     1801 taaaatattt tttcttcttc atattttcct ccaccctaag taatcctgct tttcagtgcc
     1861 tgcatgaaat caagtttatg aatttttgca taagtcatta attgtgctga aataacaaat
     1921 attacggtta aaataatcga tacgaagatg ctgattgggt taacgggttc tgccattgta
     1981 tatatgtcgg ttgtaaatgt ttctcccata taattaacta accaaagccc tacaggtata
     2041 cctgcaacta ttccaataaa agtcataatg gtattctctc taaccagcat attgaaaatc
     2101 tctttcttag aaaaacccat aaccctcaag gatgaaaatt ccaatgttct ttcgttaata
     2161 ctcataagag tcatgctata gactataaca aatcccaaaa tacctgaaaa tacaaccata
     2221 attcccatag agactgctgt tacggtagta aactcttcaa aagcttttat catatcatcc
     2281 tgtgactgaa ttgtaatttt tttcatatca tctaacttgc ccttcacgtt atctttggaa
     2341 tttaaatata caccatttat tatcccttta tccaaaaaca tattattcaa ataatcgata
     2401 ttcatatatg catttattcc aagaacctga tttacaattc ctttaacagt tacgtaaaca
     2461 tccttatctg aaataagact ctttatcaaa attctatcac cgacaattac atttaatgac
     2521 ttggctagat ttgaagataa caatattcca ttggacggca gttttatttt gtttccatca
     2581 atattcttaa agccataaaa atcagtattt tctcttaacc ctattatggt tactatttta
     2641 gattttcttc cgttttcaat ctcaaagggt aattctgtcc tgccttccat tttgtctata
     2701 cttagctgct ctgaaaattc attaagcaca ttttcattag taaatccatt aaaacttata
     2761 ttgtactgag ctttcataaa ttcgctgtaa tgtctgttaa acatcacatc tattatatca
     2821 atcatccatg ttgtcattat catcatgcca catgtaattg caacagcagc tcctatgaaa
     2881 ataaatttct ttttctctct gaaaatattc ctaagtacaa ttttccaagt aaaaggaaca
     2941 tggctccaca ttattttaat attttcaata ataattcttt ttccttcctt aggggcttca
     3001 ggcttcatag attcggcagg attaatttta ataatttccc ttacccctaa aaaacctgat
     3061 gccgtacaaa agaataatga caatataact gaaatgatta ttctataata ataaacttta
     3121 acagtaagca tgggaatatt gaaaaacacc aagtaataat tcgtcataac accggataaa
     3181 gcagttccaa taatcgagcc taaaaatcct ccaattaatc cgactatagc agcatatttt
     3241 aaataatgta ttactatttc cttatcagta aatcccaatg ccttcaatac accgattgat
     3301 gtcctgtctt ttttaacaat tcttgaaagc ataactgcaa gcataacacc ggcaaacgaa
     3361 agaaaaacaa caggaattga cttagacatc atttccaaac ctgaaatttc ctgacttaat
     3421 aaattattgc taagctgatc ttctctttta ataatccttg taacgccata cttgtctaaa
     3481 ttgtcttcaa gaaaatcaac tatttgttca atataatttt catctttgac tttaataaga
     3541 atttcattga agtttccgtt tataccatgt attttcctta aatagttttc ctctaaaaat
     3601 accactccaa aattttcagg gtcaggaagt attgtctgct
//
LOCUS       DAOSOW010000180         3639 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_25557_length_3639_cov_3.570033, whole genome shotgun sequence.
ACCESSION   DAOSOW010000180 DAOSOW010000000
VERSION     DAOSOW010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25557_length_3639_cov_3.570033"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="PLD67_05505"
     CDS             <1..1234
                     /locus_tag="PLD67_05505"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT22010.1"
                     /translation="IFDMMIETDSLAVKIVEFKPELVGITSLCIDVIKVLKLCKETKE
                     INSNIITAVGGTQAYLNPEGFYNENVDYIFEYTTRDNINILFDSLKKEEKILIDGVRT
                     RSLSYKSTGQKGRNEYMLPDRCSTNKYRNEYSYFGYKPAAIMEFGTGCKKVCDFCLRW
                     RIEGAEEKLIDIEFTKNDLRNIKEPTVMFIDNDFLCSEEKLNSFISIVKNLNFKKNYI
                     MYGSVKGVIRYEKYLKELHEIGLKALLVGYETFNDDELLKYRKSSSTDDNLKASEILR
                     KNKIDVWASFMAHPDWNKNDFISLRKYIKKLRPQITSISPLTPFPNMPMYEEFEDRLL
                     YQKEDFEKWSFGQVMIKPSKISLREYYFELLKTNLYVNIFINKNTEMIKKFGIINIFR
                     ILKGSLKAVKKYVSLMYE"
     gene            1319..1492
                     /locus_tag="PLD67_05510"
     CDS             1319..1492
                     /locus_tag="PLD67_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22011.1"
                     /translation="MDKNFEEIKEKFINASLEEKIKIYTTTQGLTVEQFKELLAYFPI
                     KYLDKLEEAVNSN"
     gene            1574..2470
                     /locus_tag="PLD67_05515"
     CDS             1574..2470
                     /locus_tag="PLD67_05515"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOT22012.1"
                     /translation="MIKDNKLKKTYIKEALGNRNYLSYRTDLILLKILVSFAVFIATY
                     FIYLDLILSLLIASLVFFIFTLINKINVNKKNEKGREILIKKVKEEYFSSKIEEINQS
                     DFELLIKLFFKNEGYNNFIKKGRNLYLAEKDGYITCIKIFKLYGGIEVEKLDVRSLLT
                     FMGNSNIRNGFLVTTSGLSKDAAELIEKFKDKFEITIIDIEGMYNLADKYNMLPDESF
                     YFKKLCEEKTVAKKDFKENILNIKKVYLYIAASIFFYMTSVWMPENKLVIYISYYFII
                     LTTINILYFLVNKYNNKKVNRG"
     gene            2572..3324
                     /locus_tag="PLD67_05520"
     CDS             2572..3324
                     /locus_tag="PLD67_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HOT22013.1"
                     /translation="MGNLMLERQLNHRTIREFKDGGIPEEVFNKLIEVARRTPTSNGM
                     QASSIIRVTDNQKRKEIADVCKQEYVARAPELLILIADQYRNYRIADEKGGYEPSARD
                     MDRFFQGFTDACLTAQNIVNAAEALDLGVVYLGSILNDAEKIIEILELPELTMPVLGL
                     GIGYPNQNPQLKPRMSMDLRLFENTYKKFDDYLEEIKEYDIEMQTYYDLREANKRVDS
                     FSNQVVTKLKNLIPKRSDMLKVMKKQGFDLNI"
     gene            3480..>3639
                     /locus_tag="PLD67_05525"
     CDS             3480..>3639
                     /locus_tag="PLD67_05525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PduL/EutD family phosphate acyltransferase"
                     /protein_id="HOT22014.1"
                     /translation="MRFKVTVGLSNKHVHLSKEHIDILFGEGYELTPIKDLTQPGQFA
                     CDEKVDLVG"
BASE COUNT         1419 a          427 c          673 g         1120 t
ORIGIN      
        1 aattttcgac atgatgattg aaactgacag cctagctgta aaaatagttg aatttaaacc
       61 ggaactggtt ggaattacat ccctttgcat tgatgttatc aaggttttaa aactttgcaa
      121 agaaacaaaa gaaataaatt caaatataat aacggctgtg gggggaactc aagcctattt
      181 gaatccggaa ggcttttata atgaaaatgt ggactatatt tttgaatata caacaagaga
      241 caacattaat attctttttg actcactaaa aaaagaagaa aaaatattga ttgacggagt
      301 taggacgagg tctttgtcat ataaatccac aggacaaaaa ggaagaaatg aatatatgct
      361 gcctgacaga tgctcaacga ataagtacag gaatgaatat tcatatttcg gctataaacc
      421 tgctgcaata atggaattcg gcacaggctg taaaaaggta tgcgactttt gtctcaggtg
      481 gagaattgaa ggtgcagaag aaaaactgat agatatagaa tttaccaaaa atgatttaag
      541 aaatattaaa gagccgacag taatgtttat tgacaatgat tttttgtgca gcgaagaaaa
      601 gctcaattca tttatcagta ttgtaaaaaa cctgaatttt aaaaagaatt atattatgta
      661 cggctccgta aaaggtgtta tccggtatga aaaatattta aaggaattgc atgaaatagg
      721 gttgaaagct cttcttgtag gctatgaaac atttaatgat gacgaattat taaaatatcg
      781 caagagctca tcgacagacg ataatctaaa ggcttcggaa atactgagaa agaacaaaat
      841 tgatgtatgg gcttctttca tggctcatcc ggactggaat aagaatgatt ttatttcttt
      901 aagaaagtat attaaaaagc ttagacctca gattacttcc ataagcccct tgactccttt
      961 tcccaatatg cccatgtatg aagagtttga agacagactt ctttatcaaa aagaagattt
     1021 tgaaaagtgg agttttggac aggtgatgat aaaaccttca aaaataagct taagggaata
     1081 ttattttgaa ttattgaaaa caaatttata tgtcaatata tttatcaata agaatacgga
     1141 aatgattaaa aaatttggaa ttattaatat atttagaatt ttaaagggct ctttaaaggc
     1201 agtaaaaaag tatgtatcat taatgtatga ataaaaaaaa taaaaaattt tcctataata
     1261 cattttgata attgtgaaat aatataaaaa tagagaaatt tcaaaggaaa ggtgtattat
     1321 ggataaaaat tttgaagaaa taaaagaaaa atttattaat gcaagcttag aagaaaaaat
     1381 taaaatttat actacaacac aaggtcttac ggtagaacaa tttaaagaat tattggcata
     1441 tttccccata aagtatttag ataagcttga agaagccgta aatagcaatt aaaatatggc
     1501 gaatgtccct gtcaattatc cctgcagcgt ataaattacc tcaatttgga aataattcca
     1561 ttgaggtgat tttatgataa aggacaataa attaaagaaa acttatataa aagaggcatt
     1621 gggaaacaga aattacttaa gctatcgcac agatttaata ctgttaaaga tacttgtaag
     1681 cttcgcagtt tttatagcaa catattttat atacttggac cttatattat ctttactgat
     1741 tgcttccttg gtattcttca tttttacttt aataaacaaa ataaatgtga acaaaaagaa
     1801 tgaaaaaggc agggaaattt taattaaaaa agtgaaagag gaatatttct catcaaaaat
     1861 tgaagaaatt aatcaaagtg attttgaatt gctgattaaa ttatttttca agaatgaggg
     1921 atacaataat tttattaaaa aaggaagaaa cttatatttg gcggaaaagg atggttatat
     1981 aacctgtata aaaatattta agctttatgg cggtattgaa gtagaaaaac ttgatgtgag
     2041 aagtttgtta accttcatgg gaaacagcaa tataagaaat ggttttttgg ttactacatc
     2101 cggattaagc aaagatgcag ctgaattaat tgaaaagttt aaagacaaat ttgaaataac
     2161 aataattgat atagaaggta tgtataattt ggcagacaaa tacaatatgc ttcctgatga
     2221 atcattttat tttaaaaaat tatgcgaaga aaagactgtt gccaaaaaag atttcaagga
     2281 aaatatacta aatataaaaa aagtatattt gtatattgca gcttcaatat tcttttatat
     2341 gacgtccgtc tggatgcctg aaaacaaatt agttatatat atttcatatt attttattat
     2401 attgacaact ataaatatat tatacttttt agtaaataaa tataataata agaaagtgaa
     2461 cagaggttaa gttgccaagt ttagtaattt aaatacgtta attaaatcag attgattata
     2521 tatgatattt tagggtatac catatatata aatataaagg agtagatgag tatgggtaat
     2581 ttaatgttag aaagacagtt aaaccacaga acaattcgtg aatttaagga tggaggaata
     2641 ccggaagaag tgtttaataa attaattgaa gtagctagac ggactcctac atcaaacgga
     2701 atgcaggcaa gctctattat tcgagtgacc gataatcaaa aaagaaaaga aattgcagat
     2761 gtttgtaaac aggaatatgt ggcaagagct cccgaactgt taattttaat agccgaccag
     2821 tacagaaatt acagaattgc ggatgaaaag ggcggttatg agccaagtgc cagggatatg
     2881 gatagattct tccagggctt tacggatgct tgcctgactg cgcaaaatat agtaaatgct
     2941 gctgaagctt tagatttggg agttgtatat ttgggaagca ttttaaatga tgcggaaaaa
     3001 attattgaga ttttagaact gccggagctt actatgccgg tgcttggact cgggataggt
     3061 tatccgaatc agaatccgca gcttaaacca agaatgagta tggatttgcg cttatttgaa
     3121 aatacatata agaaatttga tgattatttg gaagaaatta aagaatatga tatagaaatg
     3181 cagacctatt atgatttaag agaagcaaac aaaagggtgg acagcttcag caatcaggtt
     3241 gttacaaagc taaaaaatct tattccgaaa cgctcggata tgctgaaggt tatgaaaaag
     3301 caaggattcg acttgaatat ataaggtctg ttttattaca cgaagacgat tccctcggta
     3361 ggcgggggaa aaaatatggt agttatgagc aataacaaat taaattaatt aattgttgta
     3421 attttaacta ttgtatatta taatgctttt atatatacat taattttacg gaggatgtta
     3481 tgagatttaa agttacagtc ggtttatcta ataaacatgt acatttgagc aaggaacata
     3541 ttgatatact gttcggggaa ggctatgaac ttacacctat taaagattta actcaaccgg
     3601 gacagtttgc atgcgatgaa aaagttgatt tggtaggac
//
LOCUS       DAOSOW010000181         3630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_25646_length_3630_cov_3.289790, whole genome shotgun sequence.
ACCESSION   DAOSOW010000181 DAOSOW010000000
VERSION     DAOSOW010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25646_length_3630_cov_3.289790"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="PLD67_05530"
     CDS             complement(<1..259)
                     /locus_tag="PLD67_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT22015.1"
                     /translation="MMNSILRNKNKLLIILFWIFVWEILSLIIAQEIYLPSPHAAFSA
                     LMNLLISKDTYITIYYSTYRTLTGFFLSCVFGIFLGIISGIH"
     gene            complement(234..1262)
                     /locus_tag="PLD67_05535"
     CDS             complement(234..1262)
                     /locus_tag="PLD67_05535"
                     /inference="COORDINATES: protein motif:HMM:NF015995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT22016.1"
                     /translation="MKKTSFLLVVLFVLIFTAGCNNEPAETPTPDENAEETPMPEEPA
                     DVKPVNVTIAGLKGPTSIGMIKMIDEKALKSDKYNVEYIQESAPDSLTGKIINGDIQI
                     SSVPINLASVLYNKTQGKVQLLAVNTIGNLYIVGTEDISSVSQLEGKTLGMSAKGSTP
                     DFAMNYILKQAGLEGKTELNYALDHATLAQSVIAEDTRVALLPQPFVTQTTLKNSNVK
                     MLIDLNEAWNDATGGESELFTGCIIINKEFAKNNPEFVDEFLKQYENSVNWVLENQKE
                     ASVLTAKHEIMPDAVLVEKALPYCGITFRKAAEAKESLNNFYQILFDSNPASVGGSMP
                     DDEFYFKE"
     gene            complement(1264..1518)
                     /locus_tag="PLD67_05540"
     CDS             complement(1264..1518)
                     /locus_tag="PLD67_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOT22017.1"
                     /translation="MDKRIGVIGIVIEDTSQIAEVNRILHEYSHIIIGRMGIPYREKG
                     LNVISIIVDGTNDDIGALSGKLGKIKGLSVKSALSKNIRR"
     gene            complement(1667..1864)
                     /locus_tag="PLD67_05545"
     CDS             complement(1667..1864)
                     /locus_tag="PLD67_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOT22018.1"
                     /translation="MLGERLRQIRKENNLTKQELANALNVPKERIKLYETGEEIPSDF
                     YLSSFVQYFNISKEELIELIK"
     gene            2003..2440
                     /locus_tag="PLD67_05550"
     CDS             2003..2440
                     /locus_tag="PLD67_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22019.1"
                     /translation="MILTRWFQGKNNLSDVLSIRKEVFAEENREENITDFYDEFAFNA
                     LLYEDDVPAVTARLTFKEGKYFIDKLCVKKQFKGLRYEDLLVRLLVRKAVTIGAEKTY
                     IEADESIEKILENIGFTKVPNNKALMVKEGDVGGHCCGDCSEK"
     gene            2629..2700
                     /locus_tag="PLD67_05555"
     tRNA            2629..2700
                     /locus_tag="PLD67_05555"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2662..2664,aa:Glu,seq:ctc)
     gene            2792..>3630
                     /locus_tag="PLD67_05560"
     CDS             2792..>3630
                     /locus_tag="PLD67_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOT22020.1"
                     /translation="MDYKKLAELLFPNITKTIEYYENEVYPKRKLPEGAKVTRLAPSP
                     TGFIHLGNLYGAFVDERLAHQSGGVFLLRIEDTDEKRKVEGSVETIITSLEYFDLKFD
                     EGAGIEGETGDYGPYFQSNRAEIYQCCAKHLVEMGRAYPCFCTEGELEELRKEQAKEN
                     VNTGYYGKWARDRNLSLEEVQQHLNNNEEFVIRFRSEGTEEGKFEIVDAIRGRLIMPE
                     NYQDIVILKTNGIPTYHFAHVVDDHLMGITHVVRGEEWLSTLPIHYEIFKTIGWETPV
                     YCHT"
BASE COUNT         1217 a          593 c          658 g         1162 t
ORIGIN      
        1 tatgaatgcc gcttataatt cctaaaaata tgccaaagac acaggataaa aaaaatccgg
       61 tcaatgtcct gtaagtgctg taatatatag taatgtaagt atcctttgaa atcaacaaat
      121 tcatgagagc agaaaacgct gcatgaggag aagggagata tatctcctga gctattatca
      181 gcgatagtat ctcccaaacg aaaatccaaa aaagtataat caataatttg ttcttattcc
      241 ttaaaataga attcatcatc aggcatgctt cctcctaccg aagcagggtt tgaatcaaat
      301 aatatttgat aaaaattatt taagctttct tttgcttctg cagcttttct aaatgttatg
      361 ccgcagtaag gaagagcttt ttcaaccaat acggcatcag gcataatttc atgttttgcg
      421 gttaatacag atgcttcctt ttgattctcc aatacccagt taaccgagtt ttcatattgt
      481 ttcaggaatt catctacaaa ttccggatta ttttttgcaa actctttgtt tattataata
      541 catccggtga ataattcact ttctccgcct gttgcatcat tccatgcttc atttaaatct
      601 ataagcattt taacgttgga atttttcagc gttgtttgag ttacaaaagg ctgaggcaaa
      661 agcgcaaccc ttgtatcttc tgctataacg gactgtgcaa gagttgcatg gtcaagtgca
      721 taatttaact ctgttttacc ttcaagacct gcttgcttta aaatataatt catggcaaaa
      781 tcaggagttg aaccttttgc actcattcca agcgtcttgc cttccaattg tgaaacggag
      841 cttatatcct cggttcctac tatatataga tttccaatcg tatttacggc taacaactga
      901 acctttccct gagttttatt gtataaaacc gatgctaaat ttataggaac agatgatatt
      961 tgaatatcgc cgtttataat tttacccgta agtgaatcag gtgctgattc ctggatatat
     1021 tctacattgt atttatcgct tttaagtgcc ttttcatcaa tcattttaat cataccaatt
     1081 gatgtgggac ctttaagccc tgcaatagta acatttaccg gtttaacatc tgccggttct
     1141 tcaggcatgg gtgtttcttc tgcattttca tcgggtgtcg gtgtctctgc aggttcattg
     1201 ttgcagcctg ccgtaaaaat taacacaaaa agtaccacca ataaaaaact agtttttttc
     1261 atattacctc ctaatgtttt tggacaaagc actcttgacg ctgagtcctt ttattttgcc
     1321 taattttccg ctaagagctc ctatatcatc gttagtgccg tcaactataa tagagataac
     1381 attaagacct ttctccctgt aaggtatacc cattctgcct ataataatat gagaatactc
     1441 atgcaggata cggttgactt ctgcaatttg ggatgtatct tcaattacaa tgcctataac
     1501 tccgattctt ttatccatat gcacctcaat aaaaaattcc ttgcacaaca aggaataata
     1561 attaatacta taagtgtatt ccttgctctt agaagatcct taagcttaga ttatgttatt
     1621 attttaacat ataataataa ttatttcaac attattattg ttaagattat ttaatcaatt
     1681 caattagttc ttcctttgaa atattgaaat attgtacaaa acttgacaaa taaaaatcag
     1741 aaggtatttc ttctcccgtt tcataaagtt ttattctttc ttttggtaca ttcaatgcgt
     1801 ttgcaagttc ttgttttgtt aaattgtttt cttttctgat ttgccttaat ctttcgccta
     1861 acatattata cccctctcaa tattgagctt aattaattat aacatatact ttagaaaggt
     1921 aaagttaata atttagtttg ttacgtcttc ttgtcaccct tttaaataaa tgatataatt
     1981 taaaaaaacg aagaggtgca ctatgatttt aacaaggtgg tttcagggta aaaataactt
     2041 aagcgatgtt ttgagtatca ggaaagaagt atttgctgaa gaaaacagag aagaaaatat
     2101 tacggatttt tatgatgaat ttgcttttaa tgctcttttg tatgaagatg atgtgcctgc
     2161 cgtaacagca agactaacat ttaaagaagg caaatacttt attgataagt tatgtgtaaa
     2221 aaaacaattt aaaggattaa gatatgaaga tttgctggta aggctgcttg ttagaaaagc
     2281 agttacaata ggagcagaaa aaacttatat cgaagcagat gaaagtatag agaaaatatt
     2341 agaaaatata ggatttacaa aagtaccgaa taataaagca ttgatggtta aagaagggga
     2401 tgtgggcgga cattgctgtg gtgattgttc agaaaaataa tcaataaatg atccaaaccg
     2461 ggtatcatta taataataga aatatcttgg tgaataccta cccaaaaaaa tatagacaaa
     2521 gataacaggt tgttatataa ttactgaagt atagggtaca ccttcttaag cgatagcctt
     2581 tgagaaagga tgttccatac ttaggactaa ccaaaagtca caattttaga ctcattggtc
     2641 aagtggttaa gacgccaccc tctcacggtg gaaacagggg ttcgactccc ctatgggtta
     2701 tagaggtaag gatgacaagg ggacgggggt aatgtcataa tgacaacaac tccgtcccct
     2761 taaaaaaatg tcccgtacaa ggaggtttga gatggattat aaaaaattag cagaattgtt
     2821 atttccaaat ataacaaaga ctattgaata ttatgaaaat gaagtatatc ctaagagaaa
     2881 attgcctgaa ggagccaaag taacaagatt agcaccaagt ccaacaggtt ttattcattt
     2941 gggtaatctt tacggagcat ttgtggatga acgtttggca catcaaagcg gtggtgtttt
     3001 tttgcttaga atcgaagata ccgatgaaaa acgaaaagtt gaaggctctg ttgaaacaat
     3061 aataacatcc cttgaatact ttgacttgaa atttgatgag ggcgcaggaa tagaagggga
     3121 aacaggagat tacggaccat attttcaaag caacagagct gagatatatc agtgctgtgc
     3181 taaacacctg gttgaaatgg ggagagctta cccttgtttt tgcaccgaag gggaattgga
     3241 agaattaagg aaagagcaag caaaagaaaa tgtaaataca ggatattacg gtaagtgggc
     3301 gagagacaga aatttatcat tagaagaagt tcagcagcat ttaaacaaca atgaagaatt
     3361 tgttattaga tttcgctctg aaggaacaga agaagggaaa tttgaaatag tcgatgccat
     3421 aagaggacgt ttaataatgc ctgaaaatta tcaggatatt gtaattctga aaacgaacgg
     3481 tatacctaca tatcattttg ctcacgtggt agatgaccat ttaatgggca taactcatgt
     3541 tgtaagggga gaagaatggc tgtccacact tcctatccat tatgaaatat ttaaaacaat
     3601 aggttgggaa acacctgtat attgccatac
//
LOCUS       DAOSOW010000182         3629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_25656_length_3629_cov_3.274203, whole genome shotgun sequence.
ACCESSION   DAOSOW010000182 DAOSOW010000000
VERSION     DAOSOW010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25656_length_3629_cov_3.274203"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..70)
                     /locus_tag="PLD67_05565"
     CDS             complement(<1..70)
                     /locus_tag="PLD67_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008803480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminobutyryl-CoA ammonia lyase"
                     /protein_id="HOT22021.1"
                     /translation="MKSLIRLRMSSQDAHYGGNLVDG"
     gene            complement(161..1372)
                     /locus_tag="PLD67_05570"
     CDS             complement(161..1372)
                     /locus_tag="PLD67_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HOT22022.1"
                     /translation="MFNKFYKNESGQTLILAALIMVMLIGIGALTLDVGAMTFQRSSL
                     QNAADAAALAGVQNMTSINDIKKAVIDYARSNGLKVREDGAPQGGDTITINTPYKGDS
                     NKVEVIISREVKHHLAGVFGLAKSNISVRAVAEKSGSLGAAFNYAVFHGSEDEPLKFN
                     SSSINVNGDVHSNHEIQFNSLYANIKGTLEAVSELKVNSATVDIEDICRVSSIAKNSS
                     TINVKGSTILNPAPVIEMPDFSDILKAEAQTGITYTGNKTFNSGNFSFNQPIYVDGNL
                     TLNSGNFYFDRPIFVEGKLTINSTHFIGKGIIYAKDDIKVNSASFATTAGTVSLYSGK
                     NIELNSMWADINGFMYAPNGTISMNSMSSKVNGSVIGNIIKLNSGFLKVDYGDGNYDG
                     LPDGGSVRLVE"
     gene            complement(1432..2484)
                     /locus_tag="PLD67_05575"
     CDS             complement(1432..2484)
                     /locus_tag="PLD67_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22023.1"
                     /translation="MERDNNIVGILRDKDEADLDGVLKTTFGGYTKQSVQEYLSLIHK
                     QQQAAKETFSKNLQTLFDEKEELRIKNETLQARLNKLSSEYDNLSCSLKNIMLDDSEY
                     SAQNVLELKNKIVSLEEQVKVTNRENYSLERKIEQLNNEIDELKTKLEISNKEYESQK
                     EMLKSERLELKKQRDIVADLSNKLEEEKNEVKYLKGTISDGKFAELNAKIGQLSAQLS
                     AQTEVNSKLNEEIIMKDKTIDSLNDEVSLLKEKTNNLMKTLQEYNIQNDKLLVANEAL
                     EYQLQEEYKKTIALINEKSGITIDRLIAQKNLTVAEAKIAALEVQLLKTNKAVEVKEE
                     MNILKREVTGQVQDEE"
     gene            complement(2688..3014)
                     /locus_tag="PLD67_05580"
     CDS             complement(2688..3014)
                     /locus_tag="PLD67_05580"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOT22024.1"
                     /translation="MKLYVNDKLLSGNIKLADSFKTKLIGLLNKKSLDKNEGLLLINC
                     SGIHCFFMKFTIDAVYLSKDMKVIYKETVKPWRTGKFVRNCTHILELPEGMAKDIEIG
                     DYISLL"
     gene            complement(3014..>3629)
                     /locus_tag="PLD67_05585"
     CDS             complement(3014..>3629)
                     /locus_tag="PLD67_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOT22025.1"
                     /translation="IKIIIIWGNALLYFILGLVLHVSYLYLFLLPLFGAYTAFTVMRF
                     NLMFRITKRKDEMERQLPDVLDMLSVNVEAGLGFEQALLHIIEHFEGPLIDEFHITYR
                     EMSMGRSRRDALMLLGERCDIDDLKSFTGSIIQANKLGISLKNVLHSQAASIRENRRS
                     KIEEKAHKMSIKILLPMVMFIFPVIFIILMGPAVVSIVEMFGGM"
BASE COUNT          963 a          746 c          595 g         1325 t
ORIGIN      
        1 ctccatcaac taaattgccg ccgtaatgag catcttgtga gctcattcta agccttatca
       61 atgatttcat ttcttcctcc taaattatcc tcttgccact tgccctgtga caagcttatc
      121 ttaacattga atttagaagt tgtcaattct aatcattttt ttattctacc agcctcacgc
      181 tgcccccatc aggaagaccg tcataattgc cgtctccata atcgacttta agaaatccgc
      241 tattgagctt tattatattt ccaataacac ttccattgac ttttgaagac atgctgttca
      301 tggaaatagt tccgttagga gcgtacataa atccgttaat atctgcccac atactgttga
      361 gttctatatt ctttcccgaa taaagactga ctgttccggc tgttgtcgca aagctggcgc
      421 tgtttacctt gatatcatcc tttgcataaa tgataccctt gcctatgaag tgggtactgt
      481 taatggttaa ctttccttcc acaaatatgg gacggtcaaa atagaaatta ccgctgttaa
      541 gggttaagtt gccgtctacg tagataggct gattaaaaga gaaatttccg ctgttaaatg
      601 ttttgttgcc ggtatatgtt atgcctgttt gagcttcggc ttttaatatg tcggaaaagt
      661 ccggcatctc aattaccggg gcagggttta gaattgtgct tccctttaca tttatggtgc
      721 ttgagttctt agcaatgctt gaaacccgac aaatatcctc aatgtcaacg gttgcactgt
      781 ttacttttaa ttcggatacg gcttcaagcg ttcccttaat attagcataa agactgttaa
      841 attgaatttc atgattagag tgaacatcgc cgttaacatt aatgctgctg ctgttaaatt
      901 tcaacggttc atcttcggag ccgtggaaaa cggcataatt aaacgctgca cccaggctgc
      961 cggatttttc ggcaactgcc cggacggata tatttgattt tgccagtccg aaaacacctg
     1021 ctaaatgatg cttcacttct ctgcttataa taacctcgac cttatttgaa tcacccttat
     1081 aaggagtgtt tatcgtaatt gtgtctcccc cttgtggtgc tccgtcctct cttactttta
     1141 atccattgct tctggcataa tctataactg cttttttgat gtcgtttata cttgtcatat
     1201 tctgtacacc tgccaatgca gccgcatctg ccgcattctg caggctgctg cgctgaaatg
     1261 tcatcgctcc cacatccaag gtcaaggcac cgattccaat cagcataacc attatcaagg
     1321 cagctaagat taatgtttga ccggattcgt ttttataaaa tttattaaac atagtattac
     1381 gtccccccta atgctattta ccatccgccg acatcttttg cgggcaaata actattcttc
     1441 atcttgcact tggcctgtaa cttctctttt caaaatgttc atttcttctt ttacttcaac
     1501 cgctttattc gttttaagaa gctgtacttc cagtgctgca atttttgctt ctgcaacggt
     1561 taaatttttc tgtgcaatta atctgtctat ggtaatacct gatttttcgt taattaatgc
     1621 gattgttttt ttgtattctt cctgcaattg gtattccaaa gcttcatttg cgaccaacaa
     1681 tttatcattt tggatattat actcctgcaa tgttttcatt aaattgtttg ttttctcttt
     1741 aagcagagaa acttcatcat taagcgaatc aattgtttta tctttcatga taatttcttc
     1801 gtttaatttg ctgttaacct ctgtttgagc tgacagctga gcgcttaatt gtccaatctt
     1861 tgcatttaat tctgcaaatt taccgtcgga aattgttcct tttaaatatt ttacttcgtt
     1921 tttttcttct tctaatttat ttgataaatc tgccacgata tcgcgttgtt tttttagttc
     1981 aagtctttcg gatttgagca tttctttctg agattcatat tccttatttg aaatttcgag
     2041 cttggtcttc agctcgtcta tttcattgtt taactgctca atttttcttt ccagggaata
     2101 gttttcgcgg tttgttacct tgacttgttc ttccaaggat acaattttgt tttttaattc
     2161 caatacattt tgagctgagt attcggaatc atcaagcatg atattcttta atgagcagga
     2221 tagattgtca tattcggatg ataatttgtt cagacgagct tggagtgttt cattttttat
     2281 tcttaactct tccttttcat caaataaggt ttgaaggttt tttgaaaaag tttcctttgc
     2341 tgcttgctgc tgcttgtgga ttaatgataa gtattcctgc acagattgct tggtatagcc
     2401 cccaaaggtt gtttttaaaa caccgtctaa atctgcttca tctttatcgc gcaatattcc
     2461 aactatatta ttgtctcttt ccataatatt acctctatcg tttattttat ttttaatgta
     2521 tttatattct ttgtgctgac tctttgaatt atcacgggtt actacacaag gatgattgtc
     2581 atcctgagcg gaagcgaagg atctcaggtt ttataagatg agattcttcg agcaaagctc
     2641 tcagaatgac actcagatta ttcatagcaa tgacaatcac gggtttttca cagcaatgaa
     2701 atataatctc caatctcaat atcttttgcc ataccttccg gaagctccaa aatatgagtg
     2761 cagtttctga caaacttccc ggttctccac ggtttaacgg tttctttata aattactttc
     2821 atatctttgg ataagtagac tgcatcgatt gtgaatttca tgaaaaaaca atgtattccc
     2881 gaacagttta tcaagaggag cccttcattt ttgtctaagc tttttttgtt taacagtcca
     2941 atcagcttgg ttttaaagct gtcagccaat ttaatattgc cggacagcaa tttatcatta
     3001 acatataatt tcattacatc ccaccaaaca tttcaactat ggacacaacg gcaggtccca
     3061 ttaatattat aaatattacc ggaaaaataa acataaccat gggcaaaagt attttaattg
     3121 acattttatg tgccttttct tctattttgc tgcgcctgtt ttcccttatg gatgctgcct
     3181 gtgagtggag tacgttttta agagatatac ctaatttgtt ggcttgaatt atggagccgg
     3241 taaatgattt taaatcatca atatcacagc gctcgcctaa gagcataagc gcatctcttc
     3301 tgcttctgcc catggacatt tctctgtaag taatatgaaa ttcatcaatc aagggtccct
     3361 caaaatgctc gataatatgc agcagtgctt gttcaaaccc taatcctgct tctacattga
     3421 cgctgagcat atccaaaaca tccggaagct gtctctccat ttcatctttt ctttttgtta
     3481 tgcgaaacat taagttaaac cgcattacgg taaaagctgt ataggcacca aacagcggca
     3541 acagaaataa atataaataa ctgacatgta atactaagcc gagtataaaa tataaaagtg
     3601 catttcccca aataataatt atttttata
//
LOCUS       DAOSOW010000183         3615 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_25798_length_3615_cov_3.911517, whole genome shotgun sequence.
ACCESSION   DAOSOW010000183 DAOSOW010000000
VERSION     DAOSOW010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25798_length_3615_cov_3.911517"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="PLD67_05590"
     CDS             complement(<1..1208)
                     /locus_tag="PLD67_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT22026.1"
                     /translation="MIIKKYTTGRFAGIKDVKIEFKDGLNVILGSNESGKSTLVEGIH
                     SVLFKPSRIGRKTAEDKEFHSKFMPYPIGDSIDGEIDLEHNGEIYTLRKEWGADASSK
                     LTMPNKIVIKSDISINDSLKDVYIYGEGTYSSVFFLKQYYLKEAINRIVENKEATGEI
                     SSLLRSTIMELDGVSLESLGQKIQDEIDTLLKRWDIERNYPENNKGINNPYKVGFGKV
                     VESYYAKERIKLEMAKAEEAEKKFNEICVQIKKTEEKIEELKAKKESMEELENDVIQR
                     SILEPQIEQLNKDISALSKINRDWPRSEEKLKQLITELTKLNEKYKKLEEEKELSKKA
                     EEKNILEKNLANIDEINAKIKEFKAQISEIRTVTKEDIKALETNYNGMNKAEAMMKAG
                     VIMAKLNYLQ"
     gene            complement(1198..2313)
                     /locus_tag="PLD67_05595"
     CDS             complement(1198..2313)
                     /locus_tag="PLD67_05595"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT22027.1"
                     /translation="MIKIFHTADVHIGMKFNNYPEPVKGYLKEARLDVLDNMIKFANE
                     EKCNLFVIAGDLFDNITGIDKKTKDRVITALNNFQGECVLVIPGNHDYDNGMIDLWDS
                     FNKAASDKIVIINEECAYSLEDYGLNAVIFPAPCHSKHSSTNNLQWIKEKEIDDNYVN
                     IGIAHGALEGLSPDLDKNYYNMSIKELNNIPMDLWLLGHTHITYPFKSPITNEKIFNP
                     GTPEPDGLDCKHNGQAWIIIVDDNKKTNAKLIQTGTYKFKDKVYTIEDRDDLDKILEE
                     LIKDEPENTIARITLEGSVDEDVFQYRQEIYASIEKNIIYLIVEDSDFKMKITAEKIH
                     REFSDGSFPQELLLSLSDDEDTLQLAYELIMEVRNDN"
     gene            complement(2567..2764)
                     /locus_tag="PLD67_05600"
     CDS             complement(2567..2764)
                     /locus_tag="PLD67_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22028.1"
                     /translation="MYKYDYNYNKDMNYNLKVFCQSEKVNEEYMRVLLFEEKSKFLIN
                     KIAALKAMALVPIKWFLTLIK"
     gene            complement(2882..3322)
                     /locus_tag="PLD67_05605"
     CDS             complement(2882..3322)
                     /locus_tag="PLD67_05605"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HOT22029.1"
                     /translation="MELEQKIKMLQVIYAGALADSVLRMGKEGILEKVALEKREEQMH
                     TGKYRASQFGIDKPEDVFLVLSELFGCANWSVNSDNEGLKAEATKCMLCALAKKIGAQ
                     SPCNIFCLDPMEGMIRGLNPDINFEVTETLWSGQKCKVNVLNKK"
     gene            complement(3413..>3615)
                     /locus_tag="PLD67_05610"
     CDS             complement(3413..>3615)
                     /locus_tag="PLD67_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22030.1"
                     /translation="SRNKLRNFLNDECMLKNPDSKCKCRMSKWVKEIQLDEEYKKLRQ
                     TVNRINFYRASEIILTKDYLLK"
BASE COUNT          954 a          725 c          524 g         1412 t
ORIGIN      
        1 tcttgaagat aattaagctt agccataatt actcctgcct tcatcatggc ttctgcctta
       61 ttcataccgt tgtaatttgt ttcaagggct tttatatctt cttttgtaac ggttcttatt
      121 tcggatatct gtgccttgaa ctcttttatt ttagcattta tctcatcaat attagctaaa
      181 ttcttttcaa gtatattttt ttcttctgct ttttttgata attccttttc ttcttcaagt
      241 tttttatatt tttcatttag ctttgtcagt tctgttataa gctgtttcaa tttttcttcg
      301 cttcgaggcc aatcccggtt tatctttgaa agggcggata tatctttatt aagctgctca
      361 atttgaggct caaggataga cctttggatt acatcgtttt ctaattcctc catggattct
      421 ttcttggcct ttaattcttc gattttttct tcggtctttt taatctgtac gcagatttcg
      481 ttgaatttct tttcagcctc ttctgcctta gccatttcca gttttattct ttctttggca
      541 tagtagctct ccaccacttt accaaaaccc actttgtacg gattgtttat gcccttgtta
      601 ttttcaggat agtttctttc aatgtcccat ctctttaata aggtgtcaat ttcatcttga
      661 attttttgtc ccaagctttc aagagatact ccgtcaagct ccataattgt gcttcttaac
      721 aatgagctta tttcacctgt tgcttcttta ttctcaacta tcctgttaat tgcttcctta
      781 agatagtatt gctttaaaaa gaacacgctg ctatatgtcc cttcaccgta aatgtatacg
      841 tcctttaagg aatcattgat acttatgtcg ctttttatta caattttatt gggcatagtc
      901 aattttgaag aagcgtctgc tccccattct ttccttaatg tataaatttc tccattatgc
      961 tctaagtcta tttctccgtc tatgctgtcc cctatgggat aaggcataaa tttagagtga
     1021 aactctttat cctctgctgt tttcctgcca attctgctcg gcttgaaaag cactgaatga
     1081 attccttcaa ccaaagtgga ttttcctgac tcgtttgagc caagaattac attgagcccg
     1141 tctttaaact cgatttttac atccttaatg cctgcaaatc ttccggtggt atatttttta
     1201 attatcattt cttacctcca taataagctc ataagcaagc tgcaaggtgt cttcgtcatc
     1261 tgaaagtgac aaaaggagtt cctgaggaaa tgaaccgtct gaaaattctc tgtgtatttt
     1321 ttcagctgtt atcttcattt taaaatccga atcctcgact attaagtata ttatattctt
     1381 ttcaatcgag gcgtaaattt cctgcctata ctggaacacg tcttcatcaa cgctgccttc
     1441 taaggttatt cgcgctatgg tattttccgg ttcatccttt ataagctctt caagtatttt
     1501 atctaagtca tctctgtctt ctatcgtgta aactttatct ttaaatttat atgtgcctgt
     1561 ttgtattaac ttggcatttg tttttttatt gtcatcaaca ataataatcc aagcttgtcc
     1621 attgtgtttg cagtccaatc cgtcaggctc gggggttccc ggattaaata ttttttcatt
     1681 ggtaatcggt gatttaaacg ggtatgtaat atgtgtgtgc cctaatagcc acaggtccat
     1741 aggaatatta ttaagctctt taatcgacat attgtagtag ttcttatcca aatccgggga
     1801 caaaccttca agagccccgt gtgcgatgcc tatatttaca taattatcat caatttcttt
     1861 ttctttaatc cactgaaggt tattggtgct tgaatgcttt gaatggcatg gagcaggaaa
     1921 tataacggca ttaagaccgt aatcttctaa agaataggcg cactcttcgt ttataataac
     1981 aatcttatca gaagcagctt tattaaagct atcccacagg tcaatcatac cgttatcgta
     2041 atcatgattg ccgggtataa ccaacacaca ttctccttga aaattattta atgcagttat
     2101 aactcggtcc ttggtttttt tatctattcc ggttatgtta tcaaacaaat ctcctgcaat
     2161 gacaaacagg ttgcattttt cctcgtttgc aaattttatc atgttatcta agacgtctaa
     2221 tcttgcttcc ttaaggtagc cttttacagg ttcaggataa ttattgaact tcattccgat
     2281 atgtacatca gctgtatgaa aaattttaat cattttttca cctcattaat atttcgggga
     2341 tgttttgggg atgcttcttt tgttctcata gtgacaaagt acgaggtatg ttctatgttg
     2401 ttaatattat atagcagaat actgcaatta tcaacaagca gaaaataaaa agtccttcct
     2461 gaaatattgg tgccttattg gtgcctggca ccaaatacat ggcactaaaa agtaggtgcc
     2521 aagcacctgg ttggctttga tgcttggcac ctacattctt attcacctat tttataagtg
     2581 taaggaacca tttgattgga acaagagcca ttgcctttaa tgcggcgatt ttattaatta
     2641 ggaatttact tttttcttca aaaagcaata ccctcatgta ctcttcatta actttctcac
     2701 tttgacaaaa aacttttaag ttgtaattca tgtctttatt ataattatag tcatacttat
     2761 acatatcagt cctcactact gccaatacat ttccaacaat tcatttttca tatgtgcctt
     2821 tctcacaata aagttaattt caatagagca aactaacctt aggtaaatta tggaggctac
     2881 cctatttttt attaagcaca ttaactttgc atttctgacc tgaccataaa gtttctgtta
     2941 cttcaaagtt aatatccgga tttaagcctc taatcatacc ttccatcggg tctaaacaga
     3001 agatattgca tggactttga gctccaattt tttttgccaa tgcacatagc atgcactttg
     3061 ttgcttcagc ctttaagcct tcattatcgc tgttaaccga ccaatttgca caaccgaata
     3121 attctgatag cactaaaaat acatcttccg gtttatcaat tccaaattgc gatgcacgat
     3181 attttccggt gtgcatttgt tcttctctct tttctaaggc taccttttca agtattcctt
     3241 ctttccccat cctcaaaaca gagtctgcca atgctcctgc ataaattacc tgaagcattt
     3301 taattttttg ttctaattcc attttaaacc tccaaatttt ttaataaaaa aaccttccat
     3361 atttgttttc aatacattag tggaaggttt tttgtttttg tgacaactaa atttatttta
     3421 ataaataatc tttcgtaagt attatttcag aagccctgta aaaatttatt ctatttactg
     3481 tctgccttaa ttttttatat tcctcatcta gttggatttc cttaacccat ttactcattc
     3541 tgcatttgca ctttgaatca ggatttttta acatgcactc atcatttaaa aagttacgca
     3601 atttattacg tgaac
//
LOCUS       DAOSOW010000184         3598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_25949_length_3598_cov_4.032458, whole genome shotgun sequence.
ACCESSION   DAOSOW010000184 DAOSOW010000000
VERSION     DAOSOW010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25949_length_3598_cov_4.032458"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="PLD67_05615"
     CDS             <1..1512
                     /locus_tag="PLD67_05615"
                     /inference="COORDINATES: protein motif:HMM:NF024085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HOT22031.1"
                     /translation="EIIDSNSTEVLLESEFGIADDMPDIEKLVTAEGKAKLNNVTVTT
                     DMIIVDGRLIYNIIYRSNNENYTIHSMSGEIPFREEIAAEGVTEDMEALSNIFIDYID
                     ADKLSERNFLVKAVLILDTDIYMKRPVTYVSNLESDGSIQAKAKNIKYTDTIAEISEE
                     ININDAVELSKGSGEIDKIIKSEADVYITNIDELNDKMLIEGICKVGFMFIEDDELHT
                     IGYVSEEFPFTHYLELASSSENMLKDIKIAVNDISYSITENFDDEKKLIEFALPFTVN
                     ASFYDSVEKNIIMDCYSTDYELELQSEKVKLSSLKNLTNEVVKYENSFEVLTGSIKDI
                     YSVDVSPKVSEKRISDNKYIVDGFLDVNLLYLNGDINKIDKAFASLPFTAGFPIDEDE
                     ALFEIHSDITTHKCSAYRKGNNSVVLNCDINVALKFKDDDEITVIRDIAEKTPVDRSK
                     MPSLIFRVVQSGETLWDIAKNYNLSINYLKELNDIPEGNVLTPGTKIIIARMI"
     gene            complement(1583..2902)
                     /gene="dnaB"
                     /locus_tag="PLD67_05620"
     CDS             complement(1583..2902)
                     /gene="dnaB"
                     /locus_tag="PLD67_05620"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003871293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOT22032.1"
                     /translation="MNLGKIPPHNAEAEQTVLASCMIDHTAVEKVVNLLSYDDFYYEA
                     NKEIYDSIKQIHMQNIPVDAVTVFEELKKRGKIDYIGGFEYLATLTENIITSKNVEAY
                     CNIIREKSTLRKLIGASQEIIEKSYKEDDEVQRIIELAEQRIFSISQNRSINTFSEIK
                     EVLMTVFNQLEERAKSGGGITGLATGYTDLDRMTSGLQKSDLILLAARPSMGKTALAL
                     NIAMNVVKNGASVALFSLEMSEEQYVQRIISQEAMVDSTKLRTGNLDDDDWTRLINTM
                     SLISNYKIYIDDTPSVTLFEMMSKCRRLKIEKGLDLIVVDYLQLMSDGGRTDNRQQEI
                     SNISRGLKALARELDCPVLALSQLSRAPELRNDHRPVMSDLRESGAIEQDADVVMMLY
                     RDEYYNKEESEKKGITDVIITKQRNGPVGTVELAWIGQYTKFGNIQK"
     gene            complement(2924..3370)
                     /gene="rplI"
                     /locus_tag="PLD67_05625"
     CDS             complement(2924..3370)
                     /gene="rplI"
                     /locus_tag="PLD67_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOT22033.1"
                     /translation="MKVILLEDVKNVGQKGQIINAKDGYARNFLFPKKLAIEATEANL
                     KNLENQKKKKEEQEKKILEEAKVLEEELLKKTVVIKTKAGENGKLFGSITTKEISDVL
                     EKEHKILIDKKKFDLEEPIKSVGEYYVKIKLHPTVNAKLKVIVTEK"
     gene            complement(3367..>3598)
                     /locus_tag="PLD67_05630"
     CDS             complement(3367..>3598)
                     /locus_tag="PLD67_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004399546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOT22034.1"
                     /translation="LTIKDVNMSFVLVRNKEYINISGRSVGDMNVQVILERLGGGGHL
                     NMAGAQVQTDDMEEAKARLYEAIAQYKKEIKP"
BASE COUNT         1094 a          670 c          626 g         1208 t
ORIGIN      
        1 gaaattattg attcaaattc aactgaagta ttgttggaaa gcgagtttgg aattgctgat
       61 gacatgccgg atattgaaaa gctcgtaacc gcggaaggaa aagcaaagct taacaatgtt
      121 acggtaacaa cagatatgat aatagttgat ggcaggctga tatataatat tatttatcgt
      181 tcaaacaatg agaattatac aatacattcc atgtcaggcg aaataccttt cagagaagaa
      241 attgccgcag aaggcgtaac tgaagacatg gaagcattat caaatatttt tatagattat
      301 attgatgccg ataagctgtc agaaaggaat tttcttgtta aagcagtatt aatactggat
      361 actgatatct acatgaaaag acctgttacc tacgtttcaa atttagaatc tgacggaagc
      421 atccaggcta aggctaaaaa tattaaatat acggatacaa tagcagaaat atctgaagaa
      481 ataaacataa atgatgcggt agaacttagc aagggttcag gcgaaataga taaaattatt
      541 aaatcagaag ctgatgttta cataacaaat atagatgaat taaatgataa aatgctcata
      601 gagggaatat gcaaggttgg atttatgttt attgaagacg atgagcttca tactatcgga
      661 tatgtatctg aagaattccc ctttactcat tacctagagc ttgcttcttc aagtgaaaat
      721 atgttaaagg acataaaaat tgcagtaaat gatatatctt attcaataac tgaaaatttt
      781 gatgatgaga aaaaacttat agaattcgca ctgccattta cggtaaatgc atcattctat
      841 gattctgttg aaaaaaacat tatcatggac tgctattcga ctgattatga gttggagctg
      901 caatcagaaa aagtaaaatt gtcatcgctt aagaatttaa ctaatgaagt tgtcaaatat
      961 gaaaattctt ttgaagtgct tacaggctca ataaaggata tctattcggt agatgtaagc
     1021 ccgaaggttt ctgaaaagag aatatctgac aacaaataca ttgtagatgg atttttggat
     1081 gtaaatttgc tttatttaaa cggagacata aataaaatag acaaagcatt tgcctcactt
     1141 ccgtttaccg ccggcttccc gatagatgaa gatgaagcat tatttgaaat acattctgat
     1201 attacaactc ataaatgcag tgcctacaga aaaggaaaca actcggtagt tttaaactgc
     1261 gatattaatg tagcattaaa attcaaagac gatgacgaaa ttaccgtaat cagggatatt
     1321 gccgaaaaaa cccctgtaga caggagtaaa atgccaagcc tgatattcag agtggttcag
     1381 tccggagaaa ctctgtggga tattgcaaag aattataatt tatcaataaa ctacctgaaa
     1441 gagcttaatg atattcccga aggcaatgta cttacaccgg gaacaaaaat aataattgca
     1501 agaatgatat agatatgagg ttcatcgccg acgctcagga atacagtatg ttatcctgag
     1561 cgcagcgatg aatctttttt tgctactttt gtatatttcc aaactttgta tactgaccta
     1621 tccatgccag ctcaacggtt cctacgggac cgtttctttg tttggttatg attacatccg
     1681 ttataccttt tttctcggat tcttccttgt tgtaatattc atctctgtaa agcatcatca
     1741 ctacatcggc atcctgctca atagccccgg attctctcaa gtcagacata acaggtctgt
     1801 ggtcatttct aagctccggt gcacgggaaa gctgagacag ggccaatacc gggcaatcaa
     1861 gctccctggc aagagccttc agtcctcgcg atatattgga aatctcctgc tgcctgttgt
     1921 ccgtccgtcc tccgtcggac atgagctgca agtagtcaac tacaattaaa tctaaacctt
     1981 tttcgatttt aagcctcctg catttagaca tcatttcgaa taatgtaacg gatggcgtgt
     2041 catcaatata tattttgtaa tttgaaatca agctcatggt attaatgagc cttgtccaat
     2101 catcgtcgtc taaattgcct gtcctgagtt ttgtgctgtc aaccatggct tcttggctta
     2161 taattctttg aacgtattgc tcctctgaca tttcaagact gaaaagagca acagaagcgc
     2221 cgttcttaac aacattcatg gcaatattca aggcaagggc ggtttttccc attgatggtc
     2281 ttgctgcaag cagtatcaaa tcagatttct gaagtcccga agtcattctg tctaagtcag
     2341 tgtagcctgt tgcaaggcct gtaatgccgc cgccgctctt ggctctttct tcaagctggt
     2401 taaatactgt cataagaact tctttaattt cagaaaatgt attgatgctt ctgttttgtg
     2461 aaatggaaaa aattctttgc tcagccaatt ctatgattct ttgaacttca tcatcttcct
     2521 tatagctctt ctctataatt tcttgagaag cgccaataag cttacgcaag gttgattttt
     2581 ctcttattat attgcagtaa gcttcaacat tttttgaagt tattatattt tcggttagtg
     2641 tcgctaaata ttcaaatccg cctatatagt ctattttgcc tctttttttc agctcctcaa
     2701 aaacagtgac cgcatcgaca ggtatatttt gcatgtggat ttgtttaatg ctgtcatata
     2761 tttctttgtt tgcttcataa taaaaatcgt cataactcaa caaattaact actttttcga
     2821 cagctgtgtg gtcaatcata caggaagcca agactgtctg ctcagcttcc gcattatgag
     2881 gaggaatttt tcccaaatta attatatcag acattttcac ctcttacttt tctgtaacta
     2941 ttacttttaa tttagcattt accgtcggat gaagttttat tttcacatag tattcaccaa
     3001 cagatttaat aggctcttct aagtcaaatt tctttttatc gattaaaatt ttatgctcct
     3061 tctctaaaac atccgatatt tcttttgttg taatagagcc gaaaagcttt ccgttttcac
     3121 cggcctttgt ttttataaca acggttttct taagcagttc ttcttcaaga actttggctt
     3181 cttccaagat tttcttttcc tgttcttctt ttttcttttt ttgattttct aaatttttta
     3241 aattagcttc tgtagcttct atggctaatt ttttgggaaa aaggaaattg cgggcatatc
     3301 cgtctttagc attaattatt tgaccctttt gaccgacatt tttaacatct tccaatagta
     3361 ttactttcat ggtttaatct cctttttata ctgagctatg gcttcataca gtctggcttt
     3421 tgcttcttcc atgtcatcag tttgtacttg agctcctgcc atgttcaaat ggcctcctcc
     3481 tccaaggcgt tccaaaatta cctgcacatt catatcccct acggagcggc cgcttatatt
     3541 tatatattct ttgtttctta caagcacaaa actcatattt acatccttaa tcgtaagc
//
LOCUS       DAOSOW010000185         3591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_26018_length_3591_cov_4.142817, whole genome shotgun sequence.
ACCESSION   DAOSOW010000185 DAOSOW010000000
VERSION     DAOSOW010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26018_length_3591_cov_4.142817"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /gene="trpS"
                     /locus_tag="PLD67_05635"
     CDS             <1..901
                     /gene="trpS"
                     /locus_tag="PLD67_05635"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOT22035.1"
                     /translation="NEYDCYYCVVDMHAITVPKEPKDLRKNTYEVLANYIAAGLNTEQ
                     VTLFIQSHVSAHAELTWILNCFSYYGELGRMTQFKDKSKKQDSNAITAGLFTYPVLMA
                     ADILLYQADLVPVGEDQRQHLEITRDIAQRFNNRFGETFVVPEPYISTDVAKIMSLQD
                     PESKMSKSDSNENSYVLITDSPDVIRRKFKRSVTDSIGVVNYTDEQPGVKNLINIYSK
                     ISNIKPEEIVSNYESKGYAKLKEDVAEVVIEELKPIQERIDYLLKNKDYLEKIYNAGA
                     QKAEVVANKTLKKAQKKVGFIPR"
     gene            1136..2959
                     /gene="typA"
                     /locus_tag="PLD67_05640"
     CDS             1136..2959
                     /gene="typA"
                     /locus_tag="PLD67_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOT22036.1"
                     /translation="MKNKIINIAVIAHVDAGKSTLVDAFLQQSGVFRENQEVSEQIMD
                     SNDLERERGITIYSKNCSVMHNDIKINIVDTPGHADFSSEVERIIKTVDTVILLVDSI
                     EGPMPQTRFVLKKSLEIGIKPILLINKIDKKNHRAEEVVDMVFDLFVELNANDEQLDF
                     PILYGIAKQGIVQYELDEESHNINPLFDTIINQVQPYPDRDDEDLQLQVSTLAYDDYI
                     GRLGIGRVTAGTIKEGQIVSVAKHDGTVEKQKISNIYIYEGLKRSQVKEAFSGDIVIV
                     SGIANISIGDTICNENKIIPMETIKIEEPTMSMNFLINTSPFAGLSGKYVTTRHLKAR
                     LEKELEVNVGLRVEALEGALDGYKVSGRGELHLSILLENMRREGYEVAVSRPEVILKK
                     IDGIVMEPIERVIVNVPEIYSGSVISKLNLRKGIMVSMVPDGNYVKIEYLVPTRGLLG
                     YRTELINDSRGEGTMVRVFDHYEKFKGEIPQRINGVLISNDQGEAVAYSLSNLESRGI
                     LFINPGDKVYEGMIIGMNSRGEDITVNPCKTKKQSNIRSSAADDAIKLSPPKIMTLEA
                     ALEFINDDELVEITPDAIRLRKKYLTEIERRKYANKIKTSN"
     gene            complement(3110..>3591)
                     /locus_tag="PLD67_05645"
     CDS             complement(3110..>3591)
                     /locus_tag="PLD67_05645"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HOT22037.1"
                     /translation="GVKAFKKVIPNLKLKRGIYVSRKDKVGENIVTTFDIRMKEPNRE
                     PVINTAELHTIEHLGATFLRNHPVYDTRTIYFGPMGCRTGFYVIFSGNLTSLDVYEII
                     KEMFLFIMNYEGDIPGADEISCGNYQDMNLNMAKYEAAKFYNEVLLNIKDENLNYPE"
BASE COUNT         1277 a          560 c          722 g         1032 t
ORIGIN      
        1 aaatgaatat gactgttatt attgtgtagt tgatatgcat gcgataacag tacctaagga
       61 gcctaaggat ttaagaaaaa atacatatga agtattagcc aattatatag ctgccgggtt
      121 aaatacggaa caggtaacat tatttattca atcacacgtt tcggcacatg cggaacttac
      181 atggatattg aattgttttt cttactatgg ggagcttggc aggatgacac aatttaaaga
      241 caaatccaaa aagcaggatt ccaatgccat aacggcagga ctttttacct accctgtttt
      301 aatggctgct gacatacttt tatatcaagc tgatttggtt cctgtgggag aagaccaaag
      361 acagcactta gaaataacaa gggatattgc acaaagattt aataacagat tcggtgaaac
      421 atttgtagtt cctgagcctt atatttcgac cgatgtagca aaaataatga gcttgcagga
      481 ccctgagagc aaaatgtcta aatcagattc aaatgaaaat tcatatgtgc tcattaccga
      541 tagtcctgat gttataagaa ggaagtttaa gcgttcggtt acggattcaa taggcgttgt
      601 aaattatacg gatgagcagc cgggagtaaa aaatttaata aatatttact cgaaaatttc
      661 aaatataaag cctgaagaaa ttgtatccaa ttatgaaagc aagggatatg ctaaattaaa
      721 agaagatgtt gcagaagttg ttattgaaga actgaagcct atacaggaga gaatagatta
      781 tttgttgaaa aacaaagatt atcttgaaaa gatatataat gccggtgcgc aaaaagcaga
      841 ggttgtagca aataaaacct taaaaaaagc tcaaaagaaa gtcggattca taccaagata
      901 gacaggggta tcgataggac taatgacggg gactcacctt taccttcata tttgagggat
      961 taaaggacgt tccctttttt taattgagtc agggtgacga caaaaaaaac ttgcaaaatt
     1021 ttattaaaaa gattaaacta aactgtaaaa atttaataga ggggcgacat aactctaatg
     1081 cagagcaagc cctgcttagt cagaagaggg gaggattgtc cccgtagagg taattatgaa
     1141 gaataaaatt attaatatag cggtaatcgc ccatgttgat gcgggaaaat ctactctggt
     1201 ggatgctttt ttgcagcaaa gcggcgtttt tagagaaaat caggaagttt ccgagcaaat
     1261 aatggattca aatgatttgg agagagaaag aggaataacc atttattcaa aaaattgttc
     1321 ggtaatgcat aatgatataa aaattaatat agttgataca ccggggcatg ctgacttttc
     1381 atcagaagtc gaaagaatta ttaagactgt ggatacggta attttgctgg tggattccat
     1441 cgaaggacca atgcctcaaa cgcgatttgt gcttaagaaa tccctcgaaa taggaataaa
     1501 gcctattttg ttaataaata aaatcgacaa aaaaaatcac agggcagaag aagtcgttga
     1561 tatggtattt gatttattcg tagaattgaa tgcaaatgat gaacagcttg acttcccgat
     1621 actgtacggt attgcaaaac aaggaattgt tcagtatgaa ttagatgagg aaagccacaa
     1681 tattaatccc ttgtttgaca ctattataaa tcaagttcag ccttatcccg acagggatga
     1741 cgaagatttg cagctgcagg tatcaactct tgcctatgat gattatatag gcagactcgg
     1801 cataggaagg gttaccgccg gaacaataaa agaaggccag attgtttcgg ttgcaaagca
     1861 tgacggtact gttgaaaagc aaaaaatatc taatatttat atatatgaag gtttaaaacg
     1921 ttctcaggtc aaagaggcgt tcagcggcga tatagtaatt gtttcgggaa ttgccaatat
     1981 atctatcgga gacaccattt gcaatgaaaa caaaattata ccgatggaaa ccataaaaat
     2041 tgaagagccc acgatgtcaa tgaatttttt aatcaatacg tctccttttg caggtttatc
     2101 aggcaaatat gttacaacca gacatttaaa agcaaggctt gaaaaagagt tagaagtaaa
     2161 tgtggggctt agggttgaag ctttggaagg agccttagac ggttacaaag tgtcggggag
     2221 aggagaactt catttgtcaa ttcttcttga aaatatgaga agggaaggtt atgaagttgc
     2281 agtatcaaga cctgaagtta ttcttaaaaa aatagacggt atcgttatgg agccgattga
     2341 aagagtgatt gtaaatgtgc ctgaaatata ttccggctct gtaatatcaa agcttaattt
     2401 aagaaagggc attatggttt caatggtgcc tgacgggaat tacgtaaaaa ttgaatattt
     2461 ggttcctacc agaggacttt tgggttatcg cacggaattg ataaatgatt caagaggaga
     2521 aggtaccatg gtgagagtat ttgaccacta tgaaaaattt aaaggtgaaa tacctcaaag
     2581 aattaacgga gtattgatat ccaacgacca gggcgaagca gttgcttatt cattgagcaa
     2641 cttggaaagc aggggaattt tatttataaa tccgggcgac aaagtttatg aaggaatgat
     2701 tataggcatg aattcaaggg gagaagatat tactgtaaat ccctgcaaaa caaaaaaaca
     2761 atctaatatt cgttcatcgg cagctgatga tgcaataaaa ttatcacctc caaaaataat
     2821 gactttggaa gctgcattgg aatttatcaa tgatgatgag ttggtggaaa taacaccgga
     2881 tgccataaga cttaggaaaa aatatttaac cgaaatagaa agaagaaaat atgcaaataa
     2941 aataaagaca tcaaactaat ctaaaacaga tatagcagat tgtgacatag cgcagtctcc
     3001 ttcaccaaaa tatttgctcc ctatggtcgc atattttggg aagtcgttgc gaatgaccta
     3061 ccatccattt ctcagaaaaa ggactctgag aaatggggtt agggcatctt tactccggat
     3121 aattcaaatt ttcatctttt atatttaaaa gaacttcgtt ataaaattta gcagcttcat
     3181 atttagccat atttaagttc atatcctgat aattcccgca cgatatctca tcggctcccg
     3241 gaatatctcc ttcataattc attatgaata agaacatttc cttaataatt tcatatacat
     3301 ccaatgatgt caaatttccg ctaaaaataa cataaaatcc tgtcctgcat cccataggac
     3361 caaaatatat tgtgcgcgtg tcgtataccg gatgatttct taaaaatgtt gcacctaaat
     3421 gctctattgt atgaagctca gcagtattta taacaggctc cctgttaggc tccttcattc
     3481 ttatgtcaaa tgtagtaaca atattttctc caactttatc ttttctcgat acataaattc
     3541 ctctttttaa ttttaaatta ggaataactt ttttaaaagc tttaacgcca c
//
LOCUS       DAOSOW010000186         3577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_26166_length_3577_cov_4.654458, whole genome shotgun sequence.
ACCESSION   DAOSOW010000186 DAOSOW010000000
VERSION     DAOSOW010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26166_length_3577_cov_4.654458"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1105
                     /locus_tag="PLD67_05650"
     CDS             <1..1105
                     /locus_tag="PLD67_05650"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOT22038.1"
                     /translation="RLLALINALHWTERMNSEKFGLATSVNEIEKLVHEGKSVGVPTI
                     EGAYSLEEYNAIELLHQYYDLGIRVVGLCWSNSNSLGEGVNERYVDGTPSEGGLTELG
                     AKVVKEMNKMGMVVDVSHMNEETFWDVIEVAEAPIMASHSNVMGLYSHPRNLTDEQII
                     AIANTGGIVQQNFYAGYLGPTGDRDLKKLVDHIEYVIDLVGIDHVGLGSDFDGGGMPV
                     DLPNASFYYRITEELVERGYKKSEIEQILGKNTLRVIKQVQNLAEKNPDNVGQGLTII
                     PELEMGEGVSGNTPLLKAEVLRLNGSNIDESSFRVIVDGIVYTPEFNAETGELSLQVE
                     EPLVEKFHVVTFEAVNNSGKISRETRIFYIEEN"
     gene            complement(1257..1739)
                     /locus_tag="PLD67_05655"
     CDS             complement(1257..1739)
                     /locus_tag="PLD67_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HOT22039.1"
                     /translation="MNTLIAYGTKYGCAEKCAIELSNELDGMVDTVNLKETNKIELAK
                     YDRVIIGGSAYIGKIQKEVTDFINLNLNELMKKEIGLFICGMQEGEELKKEIAENFPE
                     QLLEKAISVKHFGGEFIFRKMNFFERAIVKKISKVTSDKSDIKHDNIKKLAIELCNMN
                     "
     gene            complement(1753..2139)
                     /locus_tag="PLD67_05660"
     CDS             complement(1753..2139)
                     /locus_tag="PLD67_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOT22040.1"
                     /translation="MKLVLKVLGIVFLLFVVVSVLSMFYLSRGLEEGLNVNIAPVDLI
                     LKEDGVYKGSYDFGRWSNELTITVKNNRITKIEITDDMMFVNPDVRNELFKRVMEKQN
                     TTVDAVSGGTVSCKAYLKSIENALNN"
     gene            complement(2156..2752)
                     /locus_tag="PLD67_05665"
     CDS             complement(2156..2752)
                     /locus_tag="PLD67_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015613870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOT22041.1"
                     /translation="MVTRREKEIEAMKELIISTAKEIIAREGFDNLSIRKIANKIEYS
                     PSIIYHYFKDKEEIVNIVMRRGYMKIVNAVYAADIESLTPRERLKRMTINYIEEALKM
                     PDEFLAAQINQSPQAHKHTSYLYKGASKEKPALGALYNCIKEIYRDADDEQIELKAQI
                     IAASTMGLIIKLVLEKNIGEEQRQNIINYFIEEVILKI"
     gene            complement(2902..>3577)
                     /locus_tag="PLD67_05670"
     CDS             complement(2902..>3577)
                     /locus_tag="PLD67_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOT22042.1"
                     /translation="RDAKEPIPTVVFYHGWSSNKETQRMRGFILASVGYQVVIPDAIY
                     HGERIPLADYSREAAIEYFWDVVFKNVEEYSIIKNELVSKHAADSNRIAIMGNSMGGF
                     TAGGIFTHNKDIRALIVLNGCCWWENFNRNADVTEKFERVEERVKELDPMNHLELLTD
                     RPILLLHGDSDTSVPIESQNIFYNKLSYMYKNKERIRLIEYHNLNHFVTTNMMEESIN
                     WLGRYL"
BASE COUNT         1059 a          664 c          565 g         1289 t
ORIGIN      
        1 caggttgctg gcacttatta atgcactgca ctggactgag cgtatgaatt cagagaaatt
       61 cgggttagca acgtcggtaa atgaaataga aaaattggtg catgaaggaa aatcagtagg
      121 tgttccaact atagaaggag cttattctct tgaagaatac aatgccattg aattgcttca
      181 tcaatattat gaccttggaa tcagagtcgt tggattatgt tggagcaatt ccaattcact
      241 tggagaaggt gttaatgaga ggtatgtgga tggcacccct tcagaaggcg gattaacaga
      301 actcggtgct aaagtagtta aagaaatgaa taaaatgggt atggtagttg acgtatctca
      361 tatgaatgaa gaaacatttt gggatgtcat agaagttgca gaggctccta ttatggcttc
      421 acactcaaat gttatgggtt tgtattctca tccgagaaac cttactgatg aacaaataat
      481 tgcaatagca aatacaggcg gcattgttca gcagaatttc tatgccggat atttgggacc
      541 tacaggtgac agggacctta aaaaattagt cgaccatatt gaatatgtta ttgatttggt
      601 aggcatcgat catgtcggat taggttccga ttttgatgga ggaggaatgc cggtagatct
      661 gccgaatgca tcgttctatt acagaataac cgaagaactt gtggaacgtg gttataaaaa
      721 gtccgaaata gaacaaatat tgggaaaaaa tactctgagg gtaataaagc aagttcaaaa
      781 cttagctgaa aaaaaccctg ataatgtagg tcaaggatta acaattatcc ctgaacttga
      841 aatgggagaa ggtgtaagcg gtaatacacc gctattaaaa gctgaagtac taagactcaa
      901 tggaagtaat atagatgagt caagcttcag agtaatagtt gacggtatag tttacacacc
      961 tgaatttaat gctgaaaccg gagaattaag tttacaggtt gaggaacctt tggtagaaaa
     1021 attccatgtc gtgacttttg aagctgtaaa taacagcggt aaaatttcac gcgaaaccag
     1081 aattttctat attgaagaaa attagctcat agttttgaaa aaaaaggtta ctccctattg
     1141 aatgaattga agtatataaa atgaaaatag ccttgtctat ttccattttt tctcccgttt
     1201 gtgtccgtgc aactccaaat ttgaatctta ccggagcaat tttaagattt tccctattaa
     1261 ttcatattac ataattctat tgccagtttt ttaatgttgt catgttttat gtctgatttg
     1321 tcagatgtaa ctttagaaat tttcttaact attgcccttt cgaagaagtt cattttcctg
     1381 aagataaact ctcctccaaa gtgtttaacc gatattgctt tttccaaaag ctgttcggga
     1441 aagttttcag ctatttcctt tttaagctct tccccttcct gcatgccgca aatgaataat
     1501 cctatttcct ttttcataag ctcatttaaa tttaagttta taaagtctgt tacttccttt
     1561 tgaattttac cgatataagc agaccctcct attattacac ggtcatattt tgcaagttct
     1621 attttgttag tttcttttaa gtttacagta tcaaccatgc catctagttc atttgataat
     1681 tcaattgcgc atttctccgc acaaccgtat tttgttccat aagcaattaa tgtattcatt
     1741 attttcctcc gattaattat ttaatgcatt ttcgatagat ttcagatatg ctttgcagct
     1801 gacagttcca ccggatacag catcgactgt agtattctgt ttttccataa ctcttttaaa
     1861 aagctcgttt cttacatcag gatttacaaa catcatatcg tctgttattt ctatttttgt
     1921 tattctgttg ttttttacgg ttattgtcaa ttcatttgac catctgccaa agtcatagct
     1981 tcctttataa acgccatcct ctttaagtat taaatccaca ggagcaatat ttacatttaa
     2041 tccctcttcc aatcctctgc tcaaatagaa catacttaat acagaaacaa ctacaaataa
     2101 taaaaacaca attcctaata ccttcaatac taatttcatt ttctactccc tttatttaaa
     2161 ttttcaatat tacttcttca ataaaataat ttattatatt ttgcctttgt tcttccccaa
     2221 tatttttttc aagcactaat tttattatca atcccatggt tgaagctgca attatttgtg
     2281 cttttaattc gatttgttca tcatcggcat ccctgtagat ttcctttata caattataaa
     2341 gagcccccaa tgcaggcttt tcttttgaag ctcctttata aagatacgaa gtatgcttat
     2401 gtgcttgagg tgattggttt atttgtgctg ccagaaattc atcaggcatt tttaatgctt
     2461 cctctatgta gtttattgtc attctcttaa gcctttccct tggtgttaag ctttctatat
     2521 cagctgcata aaccgcatta acaattttca tatagcctct tctcattaca atgtttacaa
     2581 tttcttcttt atctttaaaa tagtgatata tgattgaagg tgaatattca attttattgg
     2641 ctatctttct aattgacaaa ttgtcaaagc cttcccttgc aattatttcc ttggctgtcg
     2701 atatgattaa ttccttcatt gcttcaattt ctttttctct tctagtaacc atattacctc
     2761 cttcgctgaa cactgttctt taattgaaca ccgttcgtta ataaaatatc atttagataa
     2821 ctgtttgtca atactttttt ttattttttt ttaaataaaa aaatactgat accttagcat
     2881 cagtattcat aatattaatt tctacaaata tctgcccaac cagttaatgc tttcttccat
     2941 catatttgtg gtgacaaaat gatttaaatt gtgatattct attaatctaa ttctttcttt
     3001 gtttttgtac atgtaactta gtttattata aaatatattt tggctttcaa tgggcactga
     3061 agtgtcacta tcaccatgca gcagtaatat aggtctgtct gttaacagtt ctaaatgatt
     3121 catagggtcc aattctttaa ccctttcttc aactctttca aatttctctg taacatctgc
     3181 attcctgttg aaattctccc accaacagca gccgtttaac actataagtg ctcttatatc
     3241 cttgttgtgt gtaaaaattc cgccggcagt aaagccgccc atcgaattcc ccattattgc
     3301 aattctattg gagtctgcag catgctttga caccagctca ttctttataa tgctatattc
     3361 ttcaacattt ttaaatacaa cgtcccagaa atattctatc gctgcttccc ggctgtaatc
     3421 agccaacggt atcctttcac cgtgataaat ggcatccgga attactacct gatacccgac
     3481 tgaggctaat ataaatcctc tcatgcgctg agtttcttta tttgaactcc agccatggta
     3541 gaatactact gtaggtattg gctccttagc atctctc
//
LOCUS       DAOSOW010000187         3572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_26220_length_3572_cov_3.645721, whole genome shotgun sequence.
ACCESSION   DAOSOW010000187 DAOSOW010000000
VERSION     DAOSOW010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26220_length_3572_cov_3.645721"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLD67_05675"
     CDS             <1..747
                     /locus_tag="PLD67_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004080960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOT22043.1"
                     /translation="QRLSIQLRVNGKKTYAVSLDDYFVDRHLTPKNEKGEFDFETIKA
                     LDLDLFNEQLIDLMAGEEVQIPVYNFKEGSREYTREPVRLTNDHIIIIEGIHGLNDEL
                     TANIPQVNKFKIYISALTQLNIDRHNRISTSDLRLLRRIVRDYTHRGNNAIKTMELWD
                     NVIKGTEKYIFPYQENADAIFNSALVYELCVLKKYAEPIIMEIDEDSCFYSERQRLLK
                     FLSYFLPIEDESAIPNTSILREFIGESCFR"
     gene            complement(810..1961)
                     /gene="rodA"
                     /locus_tag="PLD67_05680"
     CDS             complement(810..1961)
                     /gene="rodA"
                     /locus_tag="PLD67_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOT22044.1"
                     /translation="MFKKESKLKRFDFILLITTILLCIYGFIAINSATMSKTTGSEPF
                     LKTQMIAFGLGMVALFVLIFIDYDIYGSLYIPIYVMTNILLLYTLINPVEASEWGDVR
                     SWIAIGPIVFQPSEIAKFGIIISLSKFIDIHKDNINQPVVLLKIILFAFLPIALILMQ
                     PDLGTAVVFIFFVAIMIYISGIDRKYVLSVMIIGLILIIAGLSIFFQIMEDYTLNEDY
                     RLDRIVTFFYPELDPDDTGYQVIQSKTAIGSGMLYGRGLYKGVQNQLGYLPTKETDFI
                     FAVIGEELGLVGGLVLLFMYAVLFYRLIRIAKNAANMFGSLIVTGITAMQLFHIFENI
                     GMTMGLTPVTGIPLPFISYGGTFMLVNMISIGLCLSVGMKRGKIDLNEF"
     gene            2130..2630
                     /gene="ruvC"
                     /locus_tag="PLD67_05685"
     CDS             2130..2630
                     /gene="ruvC"
                     /locus_tag="PLD67_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOT22045.1"
                     /translation="MLILGIDPGLAISGYGILNYSGNKFKVIEYGCIFTESCEEFPKR
                     LKHIYESYISIIDMYKPEAVAIEELFYNKNAKTAMAIAQARGVHLLAAENKNVPLYEY
                     TPLQIKQGIVGYGRAEKKQVQEMVRVILKLNEIPKPDDAADGLAAAICHAHSLKFAKE
                     FKINKY"
     gene            2639..3223
                     /gene="ruvA"
                     /locus_tag="PLD67_05690"
     CDS             2639..3223
                     /gene="ruvA"
                     /locus_tag="PLD67_05690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOT22046.1"
                     /translation="MISYIKGEVVKKGIDYLILDNNNIGYYINTSFSTLEKISEKEKI
                     IILTYMHIREDIIALYGFLTSDEIDLFKKLISVNGIGPRAGLSVLSTYEVNTVKEIIL
                     KEDVSGLSKVPGIGKKTASKIILELKDKVGTVEELKGSEIVVSSEMSDVIEALTSLGF
                     NYAEVKRTLGNMELSGKSENDIIKEALKNLNRQG"
     gene            3225..>3572
                     /locus_tag="PLD67_05695"
     CDS             3225..>3572
                     /locus_tag="PLD67_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT22047.1"
                     /translation="MFERENEIVARTFNSCEEELELSLRPQKLPQFIGQNKVKEVLNI
                     FIESAKIRNQPLDHVLLSGPPGLGKTTLANIISNELGVNIRVTSGPAIERAGDLAAIL
                     TNLNENDVLFIDEI"
BASE COUNT         1292 a          561 c          656 g         1063 t
ORIGIN      
        1 caaagattgt cgattcaatt acgagtgaac ggcaagaaaa catatgcagt gtccttggat
       61 gattattttg ttgacaggca tctgacacca aaaaatgaaa aaggagaatt tgattttgaa
      121 actatcaaag cgttagattt ggatcttttt aatgagcagc ttatagactt gatggcagga
      181 gaagaagttc aaatacctgt atataatttt aaggaaggta gcagggagta tacaagagaa
      241 cctgtaaggt taacaaatga ccatattata ataattgaag gtatccacgg gcttaatgat
      301 gaacttacag ctaatatacc gcaagttaat aagtttaaaa tttatataag tgcactgaca
      361 cagctaaaca tcgatagaca taatcgtatt tcgacatctg atttgagact attaagacgt
      421 attgtacgag attacaccca caggggaaac aacgcaatta aaacaatgga actgtgggat
      481 aatgtgatta aaggtacaga aaaatacatt ttcccttacc aagaaaatgc ggatgcaata
      541 tttaactcgg cattagttta tgagttgtgt gttcttaaaa aatatgcaga gcccattatt
      601 atggaaattg atgaagacag ctgtttttat tcagaaagac aaagattgtt gaagttttta
      661 agctacttcc ttcctattga agatgaaagc gctattccta atacttcaat actaagggag
      721 tttattggag aaagctgctt tagataatga taaggtgcta ggcaccattg gtggtaccat
      781 tggtggtgct tggcacctta tatacacgtt taaaattcat ttaaatcgat ttttcctctt
      841 ttcattccta cagacaggca tagacctatg gatatcatgt ttacaagcat gaaggtacct
      901 ccatagctta taaatggaag tggaattccc gttacaggcg ttagccccat tgtcattcct
      961 atattttcaa atatatggaa caactgcatt gcggtaatgc ctgttactat taatgagcca
     1021 aacatattag cagcattttt ggcaattctg ataagtctgt aaaataagac ggcatacata
     1081 aacaaaagaa ctaagcctcc aactaatcct aattcttcgc ctattaccgc aaaaataaaa
     1141 tctgtttcct tggttggaag atatcctaat tggttttgaa cacctttgta caatcctctt
     1201 ccgtacagca ttcccgaacc gattgccgtt ttagactgta ttacctgata acccgtatca
     1261 tccgggtcta attccggata gaagaaggtt acaattctgt caaggcggta atcttcattt
     1321 aaggtgtaat cttccattat ctgaaagaat atactgagtc ccgcaataat taatattaaa
     1381 ccgattatca taacagaaag cacatatttt ctgtcaatac ctgaaatata aatcattatt
     1441 gccacaaaaa atatgaatac aacggcagtt cctaaatccg gttgcattaa aattaatgct
     1501 atcggtaaaa aagcgaacaa aataattttt aataacacta cgggctggtt aatattatct
     1561 ttgtggatat caataaactt tgacagagaa attattattc caaacttagc tatttcagaa
     1621 ggctgaaaca ctatgggacc tattgctatc catgacctta catctcccca ttcggaagct
     1681 tcaacgggat ttatcaatgt gtacaaaaga agtatattgg tcatcacata tatcggaata
     1741 taaaggcttc cgtaaatatc ataatcaata aaaatcaaca caaataaagc aaccatacca
     1801 agtccgaaag ctatcatttg cgtttttaaa aagggctcgc ttccggttgt tttgctcatt
     1861 gtggcactgt ttattgctat aaatccatat atacataaaa gtattgttgt aattaacaat
     1921 ataaaatcaa accttttaag tttactttct tttttaaaca tatttactcc atgtcttcct
     1981 tgcttatact caggatgacg taattttatt agactacccc aattatatca catcttaaaa
     2041 caatggaaaa caaatatttc atgaattaag ttctttatat tagaacaaat gtatgctata
     2101 attttatata ataattgagg ggacgaaata tgctgatact tggtatagat ccggggctgg
     2161 caatatcagg ctacggaata ttaaattatt caggaaataa atttaaagta atagaatatg
     2221 gttgcatatt taccgaaagc tgtgaagaat tcccgaagag gctcaaacat atttatgaaa
     2281 gttatatatc tattatagat atgtacaagc ctgaagccgt tgcaattgag gaattgttct
     2341 ataataaaaa tgcaaaaacc gcaatggcta ttgctcaggc aaggggagtg catcttttgg
     2401 ctgcagaaaa caaaaatgtc cctctttatg aatatacacc acttcaaata aagcagggaa
     2461 tagtcggtta tgggagagct gaaaaaaaac aggtacagga aatggtaaga gtaattctta
     2521 aattaaatga aattccaaag cctgatgatg cggccgacgg attagcggca gcaatttgtc
     2581 atgcacattc gcttaaattt gcaaaagaat ttaaaattaa taaatattga gggaagatat
     2641 gattagctac ataaaaggtg aagtagtaaa aaaaggcatc gattacttga tattagataa
     2701 caataatata ggttattata taaatacttc tttttcgacc ttagaaaaaa taagtgagaa
     2761 agagaaaatt attatactta catacatgca cataagagaa gatattattg ctttatatgg
     2821 atttcttact tctgacgaga tagatttgtt taaaaaacta atcagtgtta acggaatagg
     2881 acctagagca ggactttccg tactgtcaac atatgaggta aatacagtaa aagaaattat
     2941 tctaaaagaa gatgtatccg gactgtccaa ggttccgggg ataggcaaaa aaacagcgag
     3001 caaaataatt ttggagctta aggataaggt tggaacagtt gaagaactca aaggaagcga
     3061 gattgttgta agcagcgaaa tgtccgatgt gatcgaagcc ttaacatctt tgggatttaa
     3121 ctatgccgag gtcaaaagaa cattggggaa tatggagctg tcaggcaagt ctgaaaatga
     3181 tataattaag gaagcactaa aaaatctgaa caggcaggga taatatgttt gaaagagaaa
     3241 atgaaatagt tgcaagaaca tttaattctt gtgaagagga attggagctt agcttaagac
     3301 ctcaaaaact ccctcaattt atagggcaga acaaagtaaa agaagttttg aacatattta
     3361 ttgaatctgc caaaattaga aatcagcctc ttgaccatgt attgctttca ggacctcccg
     3421 ggttagggaa gaccacatta gccaatatca tatcaaatga attaggagtt aatataaggg
     3481 taacttcagg acctgcaata gaaagagccg gtgatttagc tgcaatactt acaaatctga
     3541 atgaaaatga tgtattgttt attgatgaaa tt
//
LOCUS       DAOSOW010000188         3569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_26251_length_3569_cov_3.204041, whole genome shotgun sequence.
ACCESSION   DAOSOW010000188 DAOSOW010000000
VERSION     DAOSOW010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26251_length_3569_cov_3.204041"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /gene="dnaX"
                     /locus_tag="PLD67_05700"
     CDS             complement(<1..738)
                     /gene="dnaX"
                     /locus_tag="PLD67_05700"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOT22048.1"
                     /translation="MSHQAIYRKFRPKIFDDVLGQEHVTRTLKNQIMSQNIAHAYLFS
                     GIRGTGKTSTAKIFARAVNCLNNHEGNPCNECEICISILDETNMDVIEMDAASNNGVD
                     DIRELRDKVMFLPVKSKYKVYIIDEVHMLSKGAFNALLKTLEEPPEHLLFILATTEPQ
                     KIPATILSRCQRFDLKRINADIIVENMKKICNELEIKYEEKALKLIAANSEGAMRDAQ
                     SILDRCISFNSQVDYDTVINLLGTVNYQ"
     gene            complement(891..1091)
                     /gene="rpmE"
                     /locus_tag="PLD67_05705"
     CDS             complement(891..1091)
                     /gene="rpmE"
                     /locus_tag="PLD67_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOT22049.1"
                     /translation="MKKDIHPNYKTVTVKCACGNTFETGSVLDEIRVEICSECHPFFT
                     GKQKFVDKGGRVDRFKKKYGIE"
     gene            complement(1193..2587)
                     /gene="rho"
                     /locus_tag="PLD67_05710"
     CDS             complement(1193..2587)
                     /gene="rho"
                     /locus_tag="PLD67_05710"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOT22050.1"
                     /translation="MELRNLKLSELKELAKSKGIHDAYKYKKDELILKLNEIESDNND
                     ESKETINDSKIPEDKKETEQEMELPDKIVEEIESSGTEALKVTGILETHSDGYGFLRT
                     NNYLTSDDDVYISPSQIRRFHMQTGDKISGITRPPKQGEKFKALLYVKEINDQNPELA
                     RNRKEFDTLIPIYPDQRIKLEISPIDIAMRIIDLIAPIGRGQRGMIVAPPKAGKTTIL
                     KNIANSIAENYPEIEIIMLLIDERPEEVTDMKRNVKGDVVYSTFDELPKNHIKVAEMV
                     LERAKRLVEHGKDVVILLDSITRLSRAYNLTITPTGRTLSGGLDPGALYGPKRFFGAA
                     RNIENGGSLTILASALIETGSRMDDLIFEEFKGTGNMEIHLDRKLSEKRVFPAIDINK
                     SGTRREELLLNNEEMQTIWQLRKAMSNFSTADVTERIIEGLIRTKTNDEFVKQAAQIL
                     NIEKMKADLNDRYY"
     gene            2840..>3569
                     /locus_tag="PLD67_05715"
     CDS             2840..>3569
                     /locus_tag="PLD67_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOT22051.1"
                     /translation="MEKLIITACLTGAEVTKEQQPNLPITPDEIAEAAYECFLAGASM
                     VHVHARDEEGNPTQDYKYYKEIKEKIEAKCNIIFQPSTGGATWHTPEQRMQPIELKPE
                     MATLSMGTCNFGNDVFMNTEENIIKFAKRMYELGVKPELECFEKGMIDTSIRMAKKGI
                     LKTPMHYDFVLGVPGAMGAELRDLLYMVESIPAGSTWTAAAVGRFQLPINIAAIILGG
                     HVRTGFEDNIYYKKGELAKSNAQLV"
BASE COUNT         1060 a          664 c          573 g         1272 t
ORIGIN      
        1 ctgataattg actgtcccca ataaatttat tacggtatca taatcaactt gagaattaaa
       61 ggatatacat ctgtccaaaa tactttgagc atctctcatt gctccttcag aatttgctgc
      121 aatcaatttc aaggcttttt cttcgtactt tatttctaat tcattgcaga tttttttcat
      181 attttctaca ataatatcag cattgattct ctttaagtca aatctctggc acctggataa
      241 tattgtagca ggaatttttt gcggctccgt agttgcaaga atgaataata aatgctcagg
      301 cggctcttcc aaggttttta aaagcgcatt aaaggcgcct tttgacagca tatgtacttc
      361 atctattatg tataccttgt acttgctttt aacaggcaaa aacattacct tgtctctaag
      421 ctctctaata tcatctacgc cattatttga tgctgcatcc atttcaatta cgtccatatt
      481 ggtttcatcc aaaatgctta tacatatttc gcattcattg catggattac cttcatggtt
      541 atttaaacag ttaacagctc ttgcaaatat cttggcagtg gatgtttttc cggtgcctct
      601 tattccgctg aaaagataag catgggctat attttgagac attatttgat tcttcaatgt
      661 tctggtcaca tgctcctgac ccaagacatc atcaaatatt ttaggacgga attttctgta
      721 tattgcttga tgagacatat acaccacctg aaccctgtaa tttttttttc ttcatttttt
      781 gtattcatta tagcataaaa tattttttta aacaataagg gatattgaaa aaaggaatgt
      841 ctccaaacta aaaagcaatt taaggtttcc cctaaattgc cttaagtaca tcattcaatt
      901 ccgtacttct tcttaaatct gtcaactctt ccgcctttat caacaaattt ttgtttgccg
      961 gtaaagaaag gatggcattc tgaacatatt tcaactctaa tttcgtctaa tacagacccg
     1021 gtttcaaatg tatttccaca tgcacatttt acagtaacag ttttataatt gggatgtata
     1081 tcttttttca taccgttcac ctctttctta ctctatataa atgtattatc ttcaattaac
     1141 cttgagtatt ataacataag gttttatata atacaatgat ttatttttaa aattaatagt
     1201 atctgtcatt taaatcagct ttcatttttt cgatattcaa aatctgtgca gcttgtttta
     1261 cgaattcatc atttgtcttg gtcctaatca atccttcaat tattctttcg gtaacatctg
     1321 cagtagaaaa attgctcatt gcttttctta attgccatat tgtctgcatt tcttcattat
     1381 taagcagcaa ttcttctctt cttgttccgg atttatttat atcaatggca ggaaatactc
     1441 ttttttccga aagcttcctg tctaagtgaa tttccatatt gccggtgcct ttgaattctt
     1501 caaatattaa atcatccatc ctgctgcctg tttcaattaa agcgctggca aggattgtaa
     1561 ggcttccgcc gttttcaata tttctggcag cgccaaaaaa tctcttcgga ccgtacagag
     1621 ctcccggatc aagcccgcct gacaaggtcc ttcccgtagg ggtaatagta agattgtatg
     1681 ctctggaaag ccttgtaatg ctgtctaata aaattacaac atctttccca tgctcaacaa
     1741 gcctttttgc cctttccaat accatttcag ctactttgat gtgatttttg ggcaattcat
     1801 caaaggttga atatactacg tcacccttta catttcgttt catatccgtt acttcttcgg
     1861 gacgttcatc tattaggagc ataataattt caatttcagg atagttttcg gctatactgt
     1921 ttgctatgtt ttttaaaatt gttgttttac ctgcctttgg aggagctaca atcatacctc
     1981 tttgcccgcg tcctatgggg gcaattaaat caataattct cattgcaatg tctatgggtg
     2041 aaatttctaa cttgattctt tgatcaggat atatggggat aagcgtatcg aactcttttc
     2101 tattccttgc taattcagga ttctggtcat ttatttcttt aacataaagc aatgccttaa
     2161 atttttctcc ttgtttagga ggtcttgtaa tacctgaaat tttgtcaccg gtttgcatat
     2221 gaaatcttct gatttgagaa ggggatatgt agacatcatc atcgcttgtt aaatagttat
     2281 tagttcttaa aaagccgtag ccgtcagaat gggtttcaag tatgcctgtt acttttaagg
     2341 cttccgtacc gctggattca atttcttcaa ctattttatc aggcaattcc atttcttgtt
     2401 cagtttcttt tttgtcttcc ggaattttcg aatcgttaat cgtttcttta ctctcatcat
     2461 tattatcaga ttcaatttcg tttaatttta aaattaattc atctttctta tatttatagg
     2521 cgtcatgtat gccttttgac ttagccaatt cttttaattc agaaagcttt aaatttctaa
     2581 gctccatact acctccctat tcaattaatc aacattatgg aaaaatacgg ataagttatc
     2641 gacgagtgtt taaataatat catttaatat tataatagtc aagaaaataa ttgtttctgt
     2701 atttcccttc tctaagtaat aatactatta ttaaatattg tcactgaatt atattatgac
     2761 atgtctttaa caatcgattg actatattga atttatacga taaaataaac ataggcaaaa
     2821 aattataatg gaggttaaaa tggaaaaatt aattattact gcttgcctta cgggagcaga
     2881 agtaacaaaa gagcaacagc ctaatttgcc gattacaccg gatgaaattg ctgaagctgc
     2941 atatgagtgt ttcttggcgg gagcatccat ggtacatgtt catgccaggg atgaagaggg
     3001 caaccctact caggactata agtattacaa agaaatcaaa gaaaaaattg aagctaagtg
     3061 caacattata ttccagccat ctaccggcgg agcaacatgg cacacaccgg agcaaagaat
     3121 gcagccgata gaattaaaac ctgaaatggc aacactcagc atgggaacat gcaactttgg
     3181 caatgatgtt ttcatgaata ctgaagaaaa tattattaaa tttgcaaaac gcatgtatga
     3241 acttggtgtt aaacctgaat tggaatgttt tgaaaagggt atgattgaca catcaatcag
     3301 aatggctaaa aaaggcatac ttaaaacacc aatgcactat gactttgtgc ttggagtgcc
     3361 cggagcaatg ggagcagagc tcagggactt gttatacatg gtagaatcaa ttcctgcagg
     3421 ctctacatgg actgctgcag cagtaggaag attccagctg cctataaata tagcagctat
     3481 aatactaggc ggtcatgtac ggacaggttt tgaagataat atttattata aaaaaggcga
     3541 acttgctaaa tcaaatgctc aattagttg
//
LOCUS       DAOSOW010000189         3488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_27038_length_3488_cov_4.524031, whole genome shotgun sequence.
ACCESSION   DAOSOW010000189 DAOSOW010000000
VERSION     DAOSOW010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27038_length_3488_cov_4.524031"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="PLD67_05720"
     CDS             complement(<1..455)
                     /locus_tag="PLD67_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOT22052.1"
                     /translation="MNALGIIFSDMQDWSMTELASHRSVAAIPFGGRYRLIDFPLSNM
                     VNSGINKIYLITSSNYRSLMEHVGSGKDWDLSRKKGGILMIPPYSAGENHGLYKGRLD
                     ALKRVLDYIKFSKKNYVVLSDSDMIYDINYNKVIQYHEEKNSDITAVYI"
     gene            complement(457..1611)
                     /locus_tag="PLD67_05725"
     CDS             complement(457..1611)
                     /locus_tag="PLD67_05725"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006693346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOT22053.1"
                     /translation="MKLAKKECVVMLLAGGQGSRLGALTTKIAKPSVPFGGKYRIIDF
                     ALSNCVNSGLDTIGVLTQYQPLILNSYIGDGKSWDLDRMYGGIQILPPYQRVGDSEWY
                     LGTANSIFQNTNFIDQYSPEYVLILSGDHIYKMDYSLMIDFHKRMDADCTISVIDVPI
                     KEASRFGILSSDDSGRIIDFEEKPKRPKSTQASMGIYVFKWNKLKKYLEIDDKNPDSS
                     NDFGKDVIPLMLRSNEKLYAYKFKGYWKDVGTIDSLWQANMDLLGENGFELSEDWIIY
                     SRHAEKPPHYVGKNALINNSLVSEGAVILGTVENSVIFSGVIIEENALVKDSVIMADV
                     KINKNCVINYSIIDEMVTIGANLKIGKPKGENSEITVIPRESVINKMQEE"
     gene            complement(1598..>3488)
                     /gene="glgB"
                     /locus_tag="PLD67_05730"
     CDS             complement(1598..>3488)
                     /gene="glgB"
                     /locus_tag="PLD67_05730"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020073955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOT22054.1"
                     /translation="LFHQGTNYYSYNFLGSHIIENIGTVFRVWAPNAKNVHITGNFCN
                     WQKSAEYEMKKISDSGIWELFAPGIKEGELYKYIITAADNTIIYKADPYAFSSQTKTE
                     TASIVYNINKYKWNDTNWFEYKKRINLYEIPINIYEVNLPSWKRKENGDFYTYREMAP
                     LLINHVKKHYYTHIELMPIMEYPFDGSWGYQVSGYYSPTRRLGEPDDLKYFIDQCHQN
                     GIGVILDWVPAHFPKDEHGLYKFDGDFLYEYKDSEKREIKGWGTHKFDFGRTEVQSFL
                     ISNAVFWLDVYHADGLRVDAVSSMLYLDYDKKEGEWTPNIYGGNENLEAISFLKKLNE
                     SVFKNYPNTMMVAEESTAWPMVTKPTYLGGLGFNFKWNMGWMNDMIEYMETDPFFRKY
                     KHKNITFSLHYAFSENYILPLSHDEVVHGKKSILNKMPGPYEEKFANVRVLLGYMMAH
                     PGKKLNFMGYEIGQFTEWDYNKEIEWFMIEYEYHGKLNTYIKNLNEFYLKRSELWEVD
                     YSWSGFKWISNDDYEQNIISFKRINKNGDELIAVVNFSPTERHNYIIGVDEGIYTEVF
                     NSNDEEFGGTGTGNKDRLISSQGSVHGYNSFVSLTLPPLSIIFLEKETKYKVNKEGGK
                     IYEIS"
BASE COUNT         1025 a          706 c          533 g         1224 t
ORIGIN      
        1 ttaatataga cggcagttat atccgaattt ttttcttcat ggtattgtat taccttatta
       61 taattaatat cataaatcat atcagagtcc gataatacaa cataattttt ttttgaaaat
      121 ttgatataat ccaaaactct tttcaaggca tcgagtcttc ccttataaag cccgtggttt
      181 tctcctgcag aatagggggg aatcataagt atgccgccct tctttcttga caagtcccaa
      241 tctttaccgg aacctacatg ttccattaga gagcggtagt tgctgcttgt tatcaggtat
      301 attttattta tgccggaatt aaccatgttt gagagcggga aatctataag cctgtacctg
      361 cccccaaaag gtattgcggc aactgagcga tgagatgcta attctgtcat gctccaatct
      421 tgcatatctg agaaaataat tcctaacgca ttcatattat tcctcctgca ttttatttat
      481 aacactttca cgcggtatta cggtaatttc agagttctcc ccctttggtt tgccaatctt
      541 taagttagct ccgattgtta ccatttcatc tattattgaa taattaatta cacagttttt
      601 atttattttt acatctgcca ttatcacgga atcttttaca agagcattct cttctataat
      661 tactcccgaa aatattactg aattctctac agttccaaga attactgcac cttccgatac
      721 taatgaatta ttaataagag cattctttcc tacatagtgg gggggctttt ctgcatgcct
      781 tgaatatatt atccaatctt ctgataattc aaagccattc tcgcccaaaa gatccatatt
      841 tgcctgccat agtgaatcta tggtaccgac atccttccaa tatcccttaa atttgtaagc
      901 atacaacttt tcatttgatc ttagcattag aggaataaca tctttcccga agtcatttga
      961 tgagtcggga tttttatcat ctatttcaag atacttttta agcttgttcc atttaaatac
     1021 atatataccc atagaggctt gagtactttt tggccttttt ggtttctctt caaaatcaat
     1081 tattcttccg ctgtcatccg agcttaaaat cccgaagcgg gaagcttcct ttattggaac
     1141 atcaattacc gatatggtgc aatcggcgtc cattctctta tggaagtcaa tcattaagga
     1201 atagtccatt ttataaatat ggtcaccgga taaaataagc acatattcag gagagtactg
     1261 gtctatgaaa ttcgtgtttt gaaaaattga attagctgta cccaagtacc attccgaatc
     1321 ccctactctt tgataaggag gcagtatttg aattcctccg tacattctgt ctaaatccca
     1381 agactttccg tcacctatat acgaatttaa tattaatggt tgatattgtg tcaaaacacc
     1441 tattgtatcg aggccggagt taacacaatt agatagggca aagtcaatta ttctgtattt
     1501 gcctccaaac ggtacagaag gttttgcaat ttttgttgtt aaggctccca atctgctgcc
     1561 ctgacctccg gcaagcagca tgacaacaca ttctttttta gctaatttca taaattttcc
     1621 ctccctcttt gttaacttta tatttagttt ctttttctaa aaagattatc gaaaggggcg
     1681 gcagggtcaa tgatacaaaa gaattatatc cgtgtaccga accttgagat gatattaatc
     1741 tatccttatt tccggtgcct gtccctccaa attcctcatc attggaatta aatacttcag
     1801 tgtaaatacc ctcatcaacg ccaattatat agttatgtct ttctgtaggt gaaaaattaa
     1861 ccaccgcaat caattcatca ccattcttat ttattcgctt aaatgatata atattttgct
     1921 cataatcatc atttgaaatc catttaaagc cgctccatga ataatcaact tcccatagct
     1981 cagaacgttt cagataaaac tcgtttaaat ttttaatata tgtatttaat tttccatggt
     2041 attcatactc aatcataaac cattcaattt ccttgttata atcccattcc gtaaattgtc
     2101 ctatttcata tcccatgaag ttaagttttt tccccggatg agccatcatg tagcccaaaa
     2161 gcaccctgac atttgcaaac ttttcctcat aaggtccggg cattttattg agtatggatt
     2221 tcttgccatg aacaacttca tcatgagaca aaggaagtat atagttttca ctgaaggcat
     2281 aatgaagtga aaaggttatg tttttatgct tgtattttct gaaaaacggg tcagtttcca
     2341 tatattctat catatcgttc atccagccca tattccactt gaaattaaaa cccagtccgc
     2401 ctaaataagt gggttttgta accattggcc aggctgtgga ttcttctgct accatcatgg
     2461 tattagggta atttttaaat acggattcat taagcttttt taaaaaagaa attgcttcaa
     2521 gattttcatt ccctccatat atattgggag tccactcacc ttccttttta tcataatcca
     2581 aatataacat tgagcttacc gcatcaactc tcaagccgtc ggcatgataa acatccaacc
     2641 agaatacggc gtttgatatt aaaaatgatt gaacttcagt tctcccgaaa tcaaatttat
     2701 gagttcccca gcctttaatt tctctttttt cactatcctt gtactcgtaa agaaaatctc
     2761 cgtcgaattt atacagtccg tgttcatcct tggggaaatg agcaggaacc caatcaagaa
     2821 taacacctat cccattttga tggcattggt caatgaagta ttttaaatca tccggctcac
     2881 cgagacgcct ggttggagaa tagtaacctg aaacctggta gccccaggaa ccgtcaaagg
     2941 gatattccat aatgggcata agctctatat gggtatagta atgtttttta acatggttta
     3001 tcaatagcgg cgccatttct ctgtatgtat aaaaatcccc attttcttta cgcttccaag
     3061 agggtagatt aacttcgtaa atatttatgg gaatttcata taagttaatt ctttttttat
     3121 attcaaacca atttgtatca ttccatttgt atttgttaat attataaaca attgatgccg
     3181 tttccgtctt agtttgtgaa gaaaatgcat atggatctgc cttatatata attgtattat
     3241 ccgcagcagt aattatatat ttatacaatt caccttcctt tatgccggga gcaaacaatt
     3301 cccatatgcc gctatcagaa atttttttca tttcatactc ggcacttttt tgccaattgc
     3361 aaaaattgcc tgtaatgtgc acattttttg cattgggtgc ccatactctg aaaacggtgc
     3421 ctatgttttc tataatatgc gagcctaaaa aattatatga ataatagttt gtaccttgat
     3481 ggaataaa
//
LOCUS       DAOSOW010000190         3473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_27196_length_3473_cov_3.895260, whole genome shotgun sequence.
ACCESSION   DAOSOW010000190 DAOSOW010000000
VERSION     DAOSOW010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27196_length_3473_cov_3.895260"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="PLD67_05735"
     CDS             complement(<1..1026)
                     /locus_tag="PLD67_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HOT22055.1"
                     /translation="MLIFKLMYLIRGYVIIRLDAKNSEKLLNILQRRNIKVWDVEKKG
                     KGIKFKISYEDYLKNSTLINDIVKPESKRGFLLKLNKVKLRKGFSIGLLILIITLYLL
                     TSMVWDVEVIGTNQSNTNEILALLEKNHIKIPVSISRIEAKQLETLIHKNFKYKFVDV
                     FVEGSKLIIFVREREPDPAEIKSNEPSSIISKKNAIINKVIAKSGQPVVKEGDVVYEG
                     QTLIMGIVKNKNSDEFMMVPSDGIIYGKTYYNFEMKEEKIKSMNVPTNKSKIVYYLKI
                     NENSIKILGNKDPYQNYEYRENEIKVPILSNIADIGFIKGVYYEEVLKEIEIDKNTAQ
                     NKMIIAMY"
     gene            complement(1029..1310)
                     /locus_tag="PLD67_05740"
     CDS             complement(1029..1310)
                     /locus_tag="PLD67_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HOT22056.1"
                     /translation="MSKFNNFRSKLADELEIPNNALSDNFELRMHGNKKVIIENHLGI
                     KVYDENMVCIKTNDQDITIKGSKFKIEEINNYKVIVKGNIKEIIFSMKE"
     gene            complement(1474..1917)
                     /locus_tag="PLD67_05745"
     CDS             complement(1474..1917)
                     /locus_tag="PLD67_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOT22057.1"
                     /translation="MPLKEKLMEDLKESMKSKNKVKKDTVTMVRAAIKQKEVDERIEL
                     SDAEIIDIIAKQVKQKKDSIPDFEKGNRQDLVDLTNEEIKILLEYLPPQLSDEDLESI
                     VKEAIEKTGAQSKKDMGKLMAFIMPNVKGKADGKHVNEIVAKYIK"
     gene            complement(1929..2114)
                     /gene="rpsU"
                     /locus_tag="PLD67_05750"
     CDS             complement(1929..2114)
                     /gene="rpsU"
                     /locus_tag="PLD67_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOT22058.1"
                     /translation="MTEVKVRENESIDNALRRFKRQCALTGVMSEVRKREHYEKPSVK
                     RKKKAEAARRKKNKKAR"
     gene            complement(2222..2563)
                     /locus_tag="PLD67_05755"
     CDS             complement(2222..2563)
                     /locus_tag="PLD67_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOT22059.1"
                     /translation="MDCIFCKIANKEIPSQFVFENEYVVAFRDLAPQAPVHILAVPKI
                     HITSMNEITKENSHIVAEIFEAINKITESEGIRDSGYRVISNCGRDGCQSVQHLHFHI
                     LGGKKLSESLA"
     gene            complement(2586..>3473)
                     /locus_tag="PLD67_05760"
     CDS             complement(2586..>3473)
                     /locus_tag="PLD67_05760"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOT22060.1"
                     /translation="ISNVHDKTRVNIKIQDGCSQFCSYCIIPYLRGPIRSRNHEDIIG
                     EVKRITKNGYKEIVLNGIHISSYGKELKENYYLIDLIEDINKIEGIERIRLGSIEPNL
                     INEDFMKRYAVLDKACDHFHLSLQSGSDSVLKRMNRKYSTNEYKNNVELIRNYMPDAG
                     ITTDIIVGFPGETDEEFNETLEFVKKIKFSRIHVFKYSVREGTKAAEIKPQVNEQVKA
                     DRSKVLIELGKNISKEFMKKFIGRKLSVLIETKKNENIYEGYTTNYLKVMLKSDINVK
                     NEIINIHVTGIRNDYLLGE"
BASE COUNT          924 a          661 c          446 g         1442 t
ORIGIN      
        1 atacatagct attatcattt tattttgtgc tgtattttta tctatttcaa tttcttttag
       61 cacttcttca tagtatacac cttttataaa gccaatatca gctatatttg ataaaatcgg
      121 tacttttatt tcattctctc tatactcata gttttgatag gggtctttat tacctaatat
      181 tttaatacta ttttcattta ttttcaagta ataaacaatc ttacttttat ttgtaggcac
      241 attcatgctt tttatttttt cttccttcat ttcaaaatta taatatgtct tgccgtaaat
      301 aatgccatcg gaaggtacca tcataaactc atcggaattt ttatttttga ctattcccat
      361 aattaatgtc tggccttcat aaacaacgtc tccctcctta accacaggtt gaccgctttt
      421 agcaattact ttgtttataa tggcattttt tttggatatg atactgcttg gctcattgct
      481 tttaatttca gccgggtcag gctccctttc tcttacaaaa ataattaatt ttgagccttc
      541 cacaaagacg tcaacaaatt tatatttaaa atttttgtgt atcagtgttt ctaattgttt
      601 tgcttctata cgtgaaatgg aaaccggaat ttttatatga tttttttcta ataaggcgag
      661 tatttcattt gtatttgact gattggttcc tataacttca acatcccata ccatggaagt
      721 cagtaaataa agagttatta tcaatattaa cagacctatt gaaaagcctt ttcttaattt
      781 gactttgtta agctttagca aaaatcctct tttgctttcg ggctttacta tatcattaat
      841 tagagttgaa ttttttaaat agtcttcata tgatatttta aactttatcc ctttaccttt
      901 cttttcaaca tcccatactt taatatttct tctttggagt atattcaaaa gcttttctga
      961 gtttttggca tccaaacgga taatcacata acctcttatt aaatacatca gcttgaatat
     1021 taacatgact actccttcat actaaatatt atttctttta tattcccctt tactattact
     1081 ttgtaattat tgatttcttc tattttaaat tttgagccct ttattgttat gtcttggtcg
     1141 ttggttttta tgcataccat attttcatca taaaccttta ttcctaaatg gttttctatt
     1201 ataacttttt tgttgccatg cattctcagt tcaaaattat ctgataatgc attgttcggt
     1261 atttccagct catcggctaa tttactcctg aaattgttaa acttgctcat aactccctcc
     1321 ccgtcatctt ttaagtaatc atttttcttg ttactataaa ttatgcaggt atgtacatta
     1381 ctatgaaaaa ttaaaaaaag aacccttaaa gggtttaatt gagaatgccc tcgcgtcccc
     1441 ttgtcattaa aaaagaaccc gaatgggttc ttactacttt atatatttag ccacgatttc
     1501 attaacatgc tttccgtcag ctttgccttt tacattaggc attatgaagg ccatgagctt
     1561 gcccatatct tttttggact gagcgcctgt tttttcaatt gcttctttca caattgattc
     1621 aaggtcttca tctgacagct gaggtggtaa atattctaag agtattttga tttcttcatt
     1681 ggttagatct actagatctt gtcgattgcc tttttcaaag tcaggtattg agtctttttt
     1741 ttgtttaact tgtttagcta ttatatctat aatttcagca tcggataatt ctattctttc
     1801 gtcaacctct ttttgtttaa tagctgctct aaccattgta acagtatctt ttttgacttt
     1861 gtttttagac ttcatggatt cctttaaatc ttccatgagt ttttctttta aaggcataca
     1921 ttcacctctt atcttgcttt tttatttttc tttctgcgag ccgcttcagc cttcttttta
     1981 cgctttacgc ttggcttttc ataatgctct ctctttctta cttctgacat aacacctgta
     2041 agtgcgcatt gtctcttaaa tcttctgaga gcattatcga ttgactcgtt ttctcttact
     2101 tttacctctg tcattacttg ctaccctccc ctcgtcttct actactagtt gtcctaggac
     2161 accagcgagt ttgaaatagc atttattatt atataacata gaataagtaa atgcaagtaa
     2221 tttatgctaa ggattctgat aatttctttc cgcctaaaat atgaaaatgc aaatgttgta
     2281 ccgattgaca tccatctctg ccgcagttgc tgataactcg gtagcctgaa tccctaatgc
     2341 cctctgactc tgtaatttta tttattgctt caaaaatttc tgcaactata tggctgtttt
     2401 cctttgtaat ttcattcatt gaagtaatgt gtatcttggg tacagctaat atatgtacag
     2461 gtgcctgagg agctaagtct ctgaaagcta ccacgtattc attttcaaat acaaactgag
     2521 acggaatttc tttgtttgca attttacaga atatacaatc cattttttca cctcacccta
     2581 ctaatttatt cacccaataa ataatcgttc cttattccgg taacatgaat gttaattatt
     2641 tcattcttaa catttatatc actttttaac ataactttca agtaatttgt tgtataacct
     2701 tcataaatat tttcattttt tttggtttct atcaataccg acagcttcct gccgataaat
     2761 ttcttcatga attcttttga aatatttttg cccaattcaa taagaacctt gcttctgtca
     2821 gcttttacct gttcatttac ctgaggtttt atttctgcag ccttagtgcc ttcccttacg
     2881 gaatacttga aaacatgaat tcgagaaaat ttaatttttt ttacaaactc cagggtttca
     2941 ttgaattctt catctgtttc tcccgggaat ccgactatta tatcagttgt aattccggca
     3001 tcaggcatat aatttcttat taattctaca ttgtttttat actcattggt tgaatatttt
     3061 ctgttcattc tctttaatac actgtcgctt ccgctctgca gagacaaatg aaaatggtcg
     3121 catgctttat ctaatactgc atatcttttc ataaaatctt catttatcaa attaggctca
     3181 attgagccaa gccttattct ttcaattcct tcgattttat ttatatcttc aatcaaatca
     3241 ataagataat aattttcttt aagctctttg ccgtaggatg atatatgaat gccgttcagc
     3301 acaatttctt tataaccgtt ttttgtaatt cttttaactt cccctattat atcctcatgg
     3361 tttctgcttc ttatagggcc tcttaggtaa ggaatgatgc agtatgagca aaattgactg
     3421 cagccgtcct gtattttaat attgacacga gttttgtcgt gtacattgct tat
//
LOCUS       DAOSOW010000191         3463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_27296_length_3463_cov_3.165786, whole genome shotgun sequence.
ACCESSION   DAOSOW010000191 DAOSOW010000000
VERSION     DAOSOW010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27296_length_3463_cov_3.165786"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /gene="gatB"
                     /locus_tag="PLD67_05765"
     CDS             complement(<1..1402)
                     /gene="gatB"
                     /locus_tag="PLD67_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOT22061.1"
                     /translation="MSYELVAGLETHIELSTKTKIFCQCTTEFGGEPNTHCCPICIGM
                     PGTLPKLNKKVVEYAVKAGIALNCKIRNISKMDRKNYVYPDLPKAYQISQFDKPICYG
                     GYIKLSSGKNININRIHIEEDAGKLVHERGNTYIDYNRGGVPLIEIVTEPDFSSIDEV
                     LEYLEKLRLVMKYIGVSDVKMQEGSMRCDVNISVRKKGEEKLGTRTEIKNMNSLSFIA
                     KAMEYEFERHVDILESGGEIEQETRRYDSELNITESMRGKEDAEDYRYFKEPDLVDII
                     VSEEEIKKLKSELPQLPDEKLHVFINEYSLPEDDAELLIKYKKIAEYFEEASKDIKNK
                     KLASNYILGQIFRRMPTDSEKEECSISIPPEYLNELISLIENKKINSSMASNALEKML
                     DSGKPVREFISEKDMQGIDENTLREICLRALSENKEAVESYLGGKEKAFSSLIGYIMK
                     ETKGKADPQKAADILKE"
     gene            complement(1399..2856)
                     /gene="gatA"
                     /locus_tag="PLD67_05770"
     CDS             complement(1399..2856)
                     /gene="gatA"
                     /locus_tag="PLD67_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOT22062.1"
                     /translation="MSKIKYIQKLLKAKEVSCVELTKNYLNNIEKQNKAINAYVNVTG
                     EEAIKTAIKVDEKIAAGEDLMPLEGIPMSLKDNISTKDIETTCCSKMLKGYYPIYDAT
                     VWEILKNQNSVLLGKTNMDEFAMGSSCETSIFGTTLNPHNTDYVAGGSSGGAAASVAG
                     NMAAFGLGSDTGGSIRQPASFCGIVGFKPTYGTVSRYGVVAYASSLDQVGPITDSVED
                     AAIVYDCLSLYDPKDSTSIGSKTGTCDKLHNSIKGLKIGIADEYFEGINEDIISSIEE
                     AIKVYQDAGAEIVNFEIPKLKAFLPVYFIIACAEASSNLGRFDGIRYGYKTDSYNDVN
                     DMICKNRSEGFGKEVQKRILLGTYALSSGYYDAYYKKAQNLRYSIIQDFKNIFKSCDV
                     IITPSVPVTAFKKGEALTDPIATYMMDICTVPANIAGLPAISVPCGFDKNKMPIGMQI
                     IGNSFEDYKVFNAAYQYEKQTKKSDEISPYPEVEI"
     gene            complement(2861..3154)
                     /locus_tag="PLD67_05775"
     CDS             complement(2861..3154)
                     /locus_tag="PLD67_05775"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOT22063.1"
                     /translation="MITKEEVLKIAKLAKLSVNNDEIYKLIDDMSKIIEYADAINTVV
                     EDEDNEVEFYNINNSANAFNEDIVIDSYDRDEILKNREGGEKGYFVIRKRHRG"
     gene            complement(3163..>3463)
                     /locus_tag="PLD67_05780"
     CDS             complement(3163..>3463)
                     /locus_tag="PLD67_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit C"
                     /protein_id="HOT22064.1"
                     /translation="GAVRNHDLDIMIKAFEIAGYTIDDLQTKFTSLFNAFKYGAPPHA
                     GMAPGIDRIIMLLTGEENIREVIAFPMNNNAQDLLTNAPSKVTEQQLREVHIKLR"
BASE COUNT          898 a          733 c          528 g         1304 t
ORIGIN      
        1 tctcttttaa aatatctgca gctttttgcg ggtccgcttt ccctttcgtt tctttcatta
       61 tatacccgat taaggagctg aatgcttttt ctttccctcc aaggtaggat tcaaccgctt
      121 ctttattttc tgaaagggct ctcaggcaga tttctctgag agtattctca tctataccct
      181 gcatatcctt ttcggatata aattccctca ccggttttcc cgaatcgagc attttttcaa
      241 gagcattgct tgccatgctg gaattgattt ttttattttc aatcaatgaa atcagctcat
      301 ttaaatactc aggcggaatg gagatagagc attcttcttt ttctgaatct gttggcattc
      361 ttctgaatat ctgtcctaat atataattgg atgctaattt tttatttttt atatctttgc
      421 ttgcctcttc aaaatattcg gcaatttttt tgtattttat caaaagctcc gcatcatcct
      481 ccggaagact gtattcatta ataaatacat gcaatttttc atccggcagc tgaggaagtt
      541 cactttttaa tttttttatt tcttcttcgg aaactattat atcgactaaa tcaggctctt
      601 tgaaatacct gtagtcttcg gcatcttctt ttcctctcat actttcggta atattaagct
      661 cggaatcata tcttctcgtt tcctgctcaa tttcaccgcc gctttctaaa atgtccacat
      721 gcctttcaaa ttcatattcc attgctttgg ctataaagct gagggagttc atatttttaa
      781 tctccgttct ggtgccaagc ttttcttctc cctttttcct taccgatata ttaacgtcac
      841 atcgcattga gccttcctgc attttgacat ccgatacgcc tatatacttc ataaccagtc
      901 ttaacttttc caaatactcg agaacttcat caatgctgct aaaatcgggt tccgttacaa
      961 tttcgataag aggtactccg cccctgttgt aatcaatata agtatttcct ctttcatgga
     1021 ctaactttcc tgcatcttct tctatgtgta tcctgttaat attaatattt ttgcctgatg
     1081 aaagtttaat atatccacca taacaaatag gtttatcaaa ttgagatatt tggtatgctt
     1141 ttggtaaatc aggatataca taatttttac ggtccatttt tgaaatattt cttattttgc
     1201 agtttaaggc aatgcctgct tttacggcat attccacaac ctttttatta agtttgggaa
     1261 gagtgcccgg cattcctatg catatgggac agcaatgagt gttaggctcc cctccgaact
     1321 ctgtcgtgca ttgacagaat atctttgtct tagtagatag ttctatatgt gtttccaatc
     1381 ctgcaactaa ttcatagctc atatttccac ctccggatat ggggatattt catctgactt
     1441 ttttgtttgc ttctcgtatt gatacgctgc attaaacact ttataatctt caaagctgtt
     1501 gccgattatt tgcatcccta tgggcatctt atttttgtca aagccgcatg gaaccgaaat
     1561 tgcaggtaga ccggcaatgt ttgccggaac agtacaaata tccatcatat aagttgctat
     1621 ggggtcggtt aaagcttctc cttttttaaa tgctgtcacc ggcactgaag gagtaataat
     1681 aacatcacaa cttttaaata tatttttaaa atcttgtata attgaatatc ttaaattttg
     1741 cgccttctta taatatgcat cgtagtatcc ggaacttagc gcataagtac caagcaaaat
     1801 tcttttttgc acttccttgc cgaaaccttc actcctgttt ttgcatatca tatcatttac
     1861 atcattgtaa ctgtctgttt tatatccgta tcttatacca tcaaatcttc ctaaattaga
     1921 tgaagcttca gcgcacgcaa taataaaata aaccggaagg aaagctttca gcttaggtat
     1981 ttcaaaattt actatttctg cacccgcgtc ttggtaaact tttattgctt cctctattga
     2041 acttataata tcttcattta ttccttcaaa atactcatct gctattccta tttttagtcc
     2101 cttgatacta ttatgtaatt tatcacatgt accggttttg cttcctatgg atgttgagtc
     2161 cttagggtcg tataaggaca ggcagtcgta tactattgca gcatcttcca cagaatcggt
     2221 tattggtccc acctggtcca agcttgaagc ataagcaaca actccatatc gggaaaccgt
     2281 gccatacgta ggtttaaatc ctacaatgcc gcaaaagctt gcaggctgcc ttatggaacc
     2341 tcctgtgtcg gagcctaagc cgaatgctgc catattcccc gctacggaag ctgcagctcc
     2401 tccggagctt cctcctgcca cataatccgt gttgtgagga ttcaatgttg ttccgaagat
     2461 tgaagtttca caagatgagc ccatggcaaa ctcatccata ttggtctttc caagaagcac
     2521 agagttttgg ttctttaata tctcccaaac agtagcatca taaattggat aatatccttt
     2581 aagcattttt gagcagcatg ttgtttcaat atccttggta gaaatattgt cttttaaaga
     2641 catggggatg ccttccaaag gcattaaatc ttctccggct gcaatttttt catcaacctt
     2701 tattgctgtt ttaattgctt cttcacctgt tacgttgaca tatgcattta tagccttatt
     2761 ttgtttttca atgttgttaa ggtagttttt tgtcagttca acacatgaaa cttcctttgc
     2821 ttttaataat ttttgtatat atttaatttt gctcaatctt tcacccccta tgccttttcc
     2881 ttattacaaa atatcccttt tctccgcctt ctctgttctt taaaatttca tccctgtcat
     2941 aagaatctat aacgatatct tcgttaaaag catttgcact gttgttaatg ttataaaatt
     3001 caacttcatt atcttcatct tcaacaactg tattaattgc atctgcatat tcgattattt
     3061 tagacatatc gtcaatcagt ttataaattt catcattgtt taccgataat ttagcaagct
     3121 ttgcaatttt caaaacttct tccttagtta tcattatctc acctacctca attttatatg
     3181 aacttctctt aattgctgct ctgtaacctt tgatggtgca tttgtcagta aatcttgagc
     3241 attgttgttc atggggaaag ctataacttc tcgaatattt tcttcgcctg taagaagcat
     3301 tattattctg tcaattcccg gagccatgcc tgcgtgagga ggtgctccgt atttaaatgc
     3361 attgaacaag gaggtaaatt ttgtttgtaa atcatctatt gtataccctg ctatttcaaa
     3421 tgctttaatc ataatatcca aatcgtgatt tcttacagct ccg
//
LOCUS       DAOSOW010000192         3443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_27520_length_3443_cov_4.716057, whole genome shotgun sequence.
ACCESSION   DAOSOW010000192 DAOSOW010000000
VERSION     DAOSOW010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27520_length_3443_cov_4.716057"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="PLD67_05785"
     CDS             complement(<1..449)
                     /locus_tag="PLD67_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22065.1"
                     /translation="MYDRYMPYLANNKMLDLTYKKLKSLESPQIGYLKLNTYRHDTRS
                     PISNAFVRVSKLTISGYYREIGTGVFLAANSSDENGSVPIFELPVLTNENEMYNVSVN
                     YLDYCPAYIIDIPIYPGVTTTYDVYMHHYSMNGDTDMDYHFILQPQIP"
     gene            643..716
                     /locus_tag="PLD67_05790"
     tRNA            643..716
                     /locus_tag="PLD67_05790"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:677..679,aa:Pro,seq:cgg)
     gene            799..1317
                     /locus_tag="PLD67_05795"
     CDS             799..1317
                     /locus_tag="PLD67_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HOT22066.1"
                     /translation="MTSEERRARIEEILKNSTEPVTASYFAKKFSVSRQVIVGDIALM
                     RAGGFKITATPRGYVFDKEEESKCVFTVACCHDEKNLGDELFAIVDNGGTVQDVIVEH
                     PIYGEIIGELHIRSRYDVELFLKKIAEKKVQPLLRLTDGIHLHTIKCKDIETKNRIIN
                     VLKLKNIMLEQK"
     gene            1449..2021
                     /locus_tag="PLD67_05800"
     CDS             1449..2021
                     /locus_tag="PLD67_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012200294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22067.1"
                     /translation="MNRNNSSSMVISALLCAIGIIIPIISPLKITLEPASFTLASHVA
                     IFIAMFISPASALFVAAGTAAGFMIAGFPIVIVLRAATHIVFATLGAIYLKKNPNALN
                     SFKSSQIFSLAIGVLHGISEVLIVMPFYFGNNMSSAYYVKGFIVSVILLVGVGTVVHS
                     MVDFYLAKAIWKPVTKAVKIPIDVSVKYNA"
     gene            2155..3234
                     /locus_tag="PLD67_05805"
     CDS             2155..3234
                     /locus_tag="PLD67_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22068.1"
                     /translation="MELITASKNNRRHFLKFYRKQYLNNDLKRDTLSGMLKNLLYGKS
                     KLCGSVDMEPVMVVENNEIIMVSLLAHAHRMPEYLQICFFESEEYNMDAFNLILDRAK
                     DMAKEKKAAKLTGSLNIHVNYGLGFLASDFDKKQSFGAPHNPEFYNYYFEKSGFEIIN
                     MVSYKKDMTKSPDIINNNVREKLKEIYTVRKADFKNFKKDIEIYTKINNEAFSNHLFY
                     YTRNTEEDYELFKELKYLLRDENLLFVEKQGVPVGFMLWYPDFNEIIEKNETVGIKTV
                     LKNKLFPHRIKTFKIVEIGVIPEERNKGAILALFDYCNKCVKGKYDTMESSWILSDNL
                     KSKSFGIKWADGEFKHYKAYVKEII"
     gene            3231..>3443
                     /locus_tag="PLD67_05810"
     CDS             3231..>3443
                     /locus_tag="PLD67_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22069.1"
                     /translation="MIIYKADKLKKIKIFDLNEEYIRLLSLLEKINPCNQFYEATKDC
                     LIISYELKLNLLNFINLLPLKAKAQII"
BASE COUNT         1253 a          488 c          634 g         1068 t
ORIGIN      
        1 ggtatttgcg gctgcaaaat aaaatgatag tccatatcag tatcaccgtt catagaataa
       61 tggtgcatat acacatcata tgtggttgtt acacccggat atatcggaat atcaattata
      121 taagcagggc agtaatccag ataattaaca gatacattat acatttcatt ttcattcgtt
      181 aatacgggaa gttcaaaaat tggaaccgaa ccattttcat ctgaagaatt tgcagccaaa
      241 aaaacgcctg ttcctatttc tctataataa ccgcttatgg taagttttga tactctgaca
      301 aatgcattgg aaataggact tcttgtgtca tggcgataag tattgagctt taaataccct
      361 atttgcgggg attcgaggct ttttagtttc ttgtaagtaa ggtcaagcat tttattattt
      421 gccaaatatg gcatatatct gtcatacatt ggtaacctcc aaaaaattta gaataataca
      481 aaataaaaag tacatataat ttattattat aatatgtcaa aagtttaaat gtgttttaaa
      541 tattgatggt taatctcatg tgcagattta acgaaccgaa tgtggacaac aagaaaattc
      601 cattgacaaa aattttcata gagcttataa tgttagtgtt gtcggggtgt agcgaagctt
      661 ggtatcgcgc atggttcggg accatgaggc cgaaggttca aatcctttca ctccgatttt
      721 tcaacaaggt gccggcaaaa tatttgccgg catttgtata tgactaaatt ggtaatccct
      781 gcgtaaagga ggcttactat gacttcagag gaaagaagag cacgaattga agaaatatta
      841 aaaaatagca cagagcctgt aactgcttca tattttgcca aaaagttttc cgtaagccgt
      901 caggttatag taggagatat tgctctcatg cgggcaggcg gattcaaaat aactgcaaca
      961 ccaagaggtt atgtttttga caaagaagaa gaaagcaaat gcgtatttac agtcgcatgc
     1021 tgccatgatg aaaagaatct gggggatgaa ctgtttgcta ttgttgataa cggaggaaca
     1081 gtgcaagatg ttattgtaga gcaccctatc tatggagaaa ttatcggaga actgcacatt
     1141 aggtcaagat atgacgtaga attattctta aaaaaaattg cagaaaagaa agttcagcct
     1201 ctcttgagac ttacagacgg tatacacctc cacactatta aatgcaaaga tatagaaaca
     1261 aaaaacagaa taataaatgt attgaaatta aaaaatatta tgttagagca gaaatagcaa
     1321 tcataagaag ttatcttgat aatattatgc ttataatatc ttgacaaaat taaaaaacta
     1381 ttctataatc ctatcaacag gtgtcaagac atatgtcaag acaataatgc attggaaagg
     1441 atagaataat gaataggaat aatagtagct caatggtaat atctgctctt ttgtgcgcta
     1501 tcggaataat aataccgata atatcaccgc ttaaaataac attggagccg gcatcattta
     1561 cactggccag ccatgtggca atttttattg caatgtttat ttcacctgct tctgcattat
     1621 ttgttgcagc aggtacagca gcaggtttta tgattgcagg atttccaata gtaatagtat
     1681 tacgtgctgc aacacatatt gtttttgcaa cattaggagc aatttatttg aaaaagaatc
     1741 cgaatgcctt aaattctttt aaatcttcac aaattttttc gttggcaatc ggagtgctgc
     1801 acggaataag cgaagtgtta atagtaatgc ctttttattt tggaaataat atgagctctg
     1861 cttactacgt caaaggattt attgtttccg taattttgtt ggtgggagtt ggaactgtcg
     1921 tacacagcat ggtagacttt tatttggcaa aagcaatttg gaagccggtt acaaaggcag
     1981 ttaaaatccc gattgatgta agtgtgaaat ataacgcata ggattactca gactgcccct
     2041 tcagaatggt tcattgggaa tatattgtca tctcaagact gttaaacagt cttttttatt
     2101 ttgtaatact ttataagcta tgctataata actataaaaa tttatggagc aatcatggaa
     2161 ttaattactg caagcaagaa taacagaagg cattttttaa agttttatag aaaacaatat
     2221 ttgaataatg atttaaaaag agatacattg tcaggcatgc tgaaaaactt attgtatgga
     2281 aaatccaagc tgtgcggctc cgtagatatg gagcctgtta tggtagtgga aaacaatgaa
     2341 attataatgg tctctcttct tgcccatgcg catagaatgc ctgaatattt gcaaatatgc
     2401 ttctttgagt ctgaagaata caatatggat gcttttaatc taattttaga cagagccaaa
     2461 gatatggcaa aagaaaaaaa agctgccaaa ctaacaggct ctttgaatat acatgttaac
     2521 tacggacttg gttttttagc aagcgacttt gataaaaaac aaagtttcgg ggcaccgcac
     2581 aatcctgaat tttataatta ttattttgaa aaaagcggtt ttgaaataat taacatggtt
     2641 tcttataaaa aagatatgac aaaatctcct gatataataa ataataatgt cagagaaaaa
     2701 cttaaagaaa tttacacagt gaggaaggca gattttaaaa attttaaaaa ggatattgaa
     2761 atatatacaa aaattaacaa tgaagcattc agcaatcatt tattttatta cacaaggaat
     2821 acagaagaag attatgagct gttcaaggag ctgaagtatt tattgaggga tgaaaattta
     2881 ctttttgttg aaaaacaagg agtgccggtc ggtttcatgc tttggtatcc tgattttaac
     2941 gaaataattg aaaagaatga aactgtcggt attaagacgg tgcttaaaaa taaattattt
     3001 cctcatagaa taaaaacatt taaaatagtt gaaataggcg ttatacctga agaaagaaac
     3061 aagggtgcaa tacttgcatt atttgattat tgcaataaat gcgttaaggg aaaatatgat
     3121 actatggaat caagctggat actttcagat aatctaaagt caaaaagttt tggcataaaa
     3181 tgggctgatg gtgaattcaa acattataaa gcatacgtta aggaaattat atgataatat
     3241 ataaagcgga taaacttaaa aaaataaaaa tatttgattt aaatgaagaa tatataagac
     3301 tcttgtcctt gttagaaaaa ataaacccct gcaatcagtt ttatgaagca accaaggact
     3361 gtttaataat aagctatgag ctaaagttga atttattaaa ttttattaat cttttgccat
     3421 taaaagcaaa agctcaaatt att
//
LOCUS       DAOSOW010000193         3442 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_27534_length_3442_cov_3.657219, whole genome shotgun sequence.
ACCESSION   DAOSOW010000193 DAOSOW010000000
VERSION     DAOSOW010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27534_length_3442_cov_3.657219"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /gene="rapZ"
                     /locus_tag="PLD67_05815"
     CDS             <1..786
                     /gene="rapZ"
                     /locus_tag="PLD67_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOT22070.1"
                     /translation="NYYCMDNLPPALLPNFAELCKSSSMDVNKVAVVADIRGGIFFKD
                     LFNSLKELKNKGIEYRILFLDASDDDLIKRFKEQRRPHPLSSDGTIIEGILEERNRLE
                     EVKKKSDYIIDTTSMTLGRLKEEILNIFVKGSISHNLNITIVSFGYKYGLPKESDLVF
                     DVRFLPNPFYIDELKNSTGNDENVKDYVMSFETTSIFLEKLMDILKFLLPLYIKEGKS
                     SLVISIGCTGGKHRSVTISNYLASMLTAENYRVLLNHRDAGKN"
     gene            787..1752
                     /gene="yvcK"
                     /locus_tag="PLD67_05820"
     CDS             787..1752
                     /gene="yvcK"
                     /locus_tag="PLD67_05820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HOT22071.1"
                     /translation="MKIVVFGGGTGLSVLLRGLKNYTKDISAIVTVADNGGGSGVLRE
                     DLGMLPPGDMRNCIIALSDIEPIMIKLMQHRFSDGYLKGQSFGNLFIAAMYEIFGNYE
                     HALKEIGSIFRLSGKVLPMTLQDAQLKAYLANGDCILGEKYIPEYVSKLNTKIDKIEL
                     VPYVCKPLEETVEDIKEADLIVLGPGSLYTSVIPNILVEGIPSLLAESNATVFYVCNL
                     MTQPGETDNYNVLDHVNAIIKHSMKYMIDYVIVNDEKISEELILNYKYQGAKQVLIDD
                     EQIKELQEMNIKVICSNFVDIRKNYIRHNSDKIGQLLLNYINEKK"
     gene            1767..2852
                     /locus_tag="PLD67_05825"
     CDS             1767..2852
                     /locus_tag="PLD67_05825"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOT22072.1"
                     /translation="MDKYKSIDPNQISIADGMIDWVRVVSKDNMVLYANKKMQEDLGE
                     NIIGKKCYDVLCRYNRCENCISSTTLEKGSIMRKHHKLGNKTYMIISSPLFDDKGEIE
                     ASVEVFRDITNEVLLSEKLQEKNKKMSDDIKFAKNMQNRMLPPKGMYNGLSIDYLYES
                     SESLSGDYFDVFEIDENNTGIYICDVVGHGVTASLLTIFVRQSLRTIAKGNINLNKIM
                     KELHKTFLSLNLDYDKYFSVFFGIYNKDSHVFKYVNAGHNSVPILISGKDIKKLMLKG
                     YPIANIFDNVDYIVEKVTLKVGDKILLYTDGITETTNSEGVQFGVDRLINTIISDGNI
                     LENIKSSVNNFSIVHKDDYAVLLAEII"
     gene            2964..>3442
                     /locus_tag="PLD67_05830"
     CDS             2964..>3442
                     /locus_tag="PLD67_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOT22073.1"
                     /translation="MKNITLDLRGSFIDEDELKKIKPEIMAAQETLLDRKGVSSEMTG
                     WLDYMYRIDKNEYEEIKAAAKYIKENSEAFILLGIGGSYLGAKAAIEAIRGDFHNELL
                     SPKVYYAGQNLSGAYLKKLIELVKEKEVCIIVISKSGTTLETALSFRVFKELMEKKYG
                     "
BASE COUNT         1278 a          468 c          709 g          987 t
ORIGIN      
        1 aattattatt gcatggataa tctgcctcct gccctccttc cgaattttgc cgagttatgc
       61 aagagttcat cgatggacgt taacaaagtg gcagttgttg cggatataag aggaggtatc
      121 ttttttaaag atttgttcaa cagtttaaaa gaactgaaaa ataagggaat tgaatacaga
      181 atactttttc ttgatgcctc tgatgatgac ttaatcaaaa ggtttaagga gcagagaaga
      241 cctcatcctt tgtcttcaga cggtacgata attgaaggaa tcctggaaga aagaaacaga
      301 ttggaagaag taaaaaagaa gtctgattat ataatagata caaccagcat gacgcttgga
      361 aggcttaagg aagaaatttt aaatattttt gtgaaaggca gcatatccca taatttaaac
      421 ataacaatag tttcattcgg ctataaatac ggtcttccta aggaatcgga cctggttttt
      481 gatgtaaggt ttctgccaaa tccattttat atagatgaat taaaaaactc aaccggcaat
      541 gatgaaaatg ttaaagatta tgtaatgagt tttgaaacga catccatatt tttggaaaag
      601 ttaatggaca ttcttaaatt tcttctgcct ttgtatataa aggaaggaaa atcaagtctt
      661 gtaatatcaa tagggtgtac cggaggcaag catcgctctg ttacaatatc aaattattta
      721 gcttccatgc ttacggctga aaattacaga gttttattaa atcacagaga tgcggggaaa
      781 aattaaatga aaatagtagt attcggcgga gggacaggcc tttctgtatt gctcagagga
      841 ttaaaaaatt acacaaaaga catatcagca attgtaaccg ttgcagataa cggcgggggt
      901 tccggagttt taagagaaga cttaggcatg ctgccgcccg gagatatgag aaactgcatt
      961 attgctcttt cagatattga gcccattatg ataaaattga tgcagcaccg tttcagcgac
     1021 ggatatttaa aggggcaaag cttcgggaat ttatttatag ctgcaatgta tgaaatattc
     1081 ggcaattacg aacatgcttt aaaggaaata ggaagtattt tcaggctttc gggaaaagtt
     1141 cttcccatga ctctgcagga tgcccaatta aaagcgtatc tcgctaacgg cgactgcata
     1201 ttgggagaaa aatatattcc tgaatatgtg agcaaattaa acacgaaaat agataaaatc
     1261 gaattggtgc cttatgtgtg caaacccctg gaagaaacgg ttgaggatat aaaagaagca
     1321 gatcttattg ttttaggacc gggaagcttg tataccagtg taattccaaa tattttagtt
     1381 gaaggcatac cgtctctttt ggctgaaagt aatgcaaccg tattttatgt atgcaatctt
     1441 atgacacagc cgggagaaac agataattac aatgttttag accatgtaaa tgcaataata
     1501 aaacacagca tgaaatacat gattgattat gtaattgtca atgatgaaaa aatatccgaa
     1561 gagttgattt taaattataa ataccagggt gcaaaacagg tacttattga tgacgagcag
     1621 ataaaagaac ttcaagaaat gaatataaag gttatatgca gcaattttgt cgatataaga
     1681 aaaaactata tcagacataa ttcagataaa ataggacagc tcttgttaaa ctatattaat
     1741 gaaaaaaaat aaaagggggt gcttctatgg ataaatataa gagtatagat ccaaatcaga
     1801 taagcatagc ggatggtatg attgattggg taagagtagt cagcaaagac aacatggttt
     1861 tgtatgccaa caaaaaaatg caggaagatt taggagaaaa tattatcggg aaaaaatgtt
     1921 atgacgtttt atgcagatat aatagatgcg aaaactgtat ttcaagcaca accttggaaa
     1981 agggctccat aatgcgcaaa catcataaat tgggaaacaa aacatatatg attatatcct
     2041 cacccttatt tgatgataag ggtgaaattg aagcttcggt agaggtattc agggatataa
     2101 cgaatgaagt tttactttcg gaaaagttac aagaaaaaaa caaaaaaatg agtgatgata
     2161 taaagtttgc aaaaaatatg caaaatcgca tgcttcctcc aaaaggaatg tataacggct
     2221 tgagcattga ctatttgtat gaatcatcag aatcattaag cggagattat tttgatgtat
     2281 ttgaaataga tgaaaataat acaggtatat atatttgtga tgttgtgggg cacggagtaa
     2341 cggcatctct cttgaccatt tttgtaaggc aatcattaag gactattgcc aaaggaaata
     2401 ttaatttgaa taaaattatg aaggagctgc ataaaacctt tttatcgttg aatttggact
     2461 atgataaata tttttcagta ttttttggaa tatataataa agattcccat gtatttaaat
     2521 atgtcaatgc aggacataac tcggtaccga ttttaataag cggtaaagat attaaaaagc
     2581 ttatgcttaa gggatatcct attgcaaata tttttgataa tgttgattac attgtagaaa
     2641 aagttacatt aaaagtcggt gataaaatac tcctttacac tgacggcata acggagacaa
     2701 caaatagcga aggggtgcaa ttcggcgtcg atagattgat aaatacaata atatccgatg
     2761 gaaatatatt agaaaacata aagtcatctg ttaataattt cagcatagtt cataaagatg
     2821 attatgcagt cttattagcc gaaataatat aaacattctg agcgatagcg aaatatttca
     2881 tgagattatt cactgcgttc agaatgacat ggtaacagta gctattgtat ggagaaaggg
     2941 aaggacaatt gggaggggag aaaatgaaaa atattactct tgatttacga ggaagcttta
     3001 ttgatgagga tgaattaaaa aaaattaaac cggaaatcat ggcagctcag gagacacttc
     3061 tggacagaaa aggagtcagc agcgaaatga caggctggct cgattatatg tacagaattg
     3121 acaaaaatga atacgaagaa attaaagctg cagcaaaata tattaaagaa aactctgaag
     3181 ctttcatact tttgggaata ggaggctcct atttaggggc aaaagctgca atcgaagcta
     3241 taaggggaga ttttcacaat gaattgttaa gtcctaaggt atattacgca ggacaaaatt
     3301 taagtggagc atatttgaaa aaacttattg agcttgttaa agaaaaagaa gtttgcatta
     3361 ttgtaatttc aaagtcggga acaaccttgg aaacagcatt gtcattcaga gtctttaagg
     3421 agctgatgga gaaaaaatac gg
//
LOCUS       DAOSOW010000194         3417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_27780_length_3417_cov_3.042534, whole genome shotgun sequence.
ACCESSION   DAOSOW010000194 DAOSOW010000000
VERSION     DAOSOW010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27780_length_3417_cov_3.042534"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="PLD67_05835"
     CDS             complement(<1..1194)
                     /locus_tag="PLD67_05835"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOT22074.1"
                     /translation="MTKNKRIVGFIFGILAAITINLLPLRGISPQGKMSLGLTLMTVL
                     FWTFHTVQSGYASGLYLVLLIIFKVAEPSVVFSPFLSSTIYLVIGAYLIASAVKLSGL
                     GERIAYAFIIKFVHSYKSIIYSIFMLTFILSIIIPHPWPRAFIIMSVMDVVIKSANIA
                     SGDKSKIGFAVFAAAVPISMIFLTGDSVINPLTVQASGESISYIQWFIFMGPPNIIAS
                     ILTCALILILFKPTSEVKINKAEIKNKLITLGALSKIEKRTVVWLIIAVVLWMTDFIH
                     GIEIGWITFLIAMLMSFPVIGAVLSPKQWQEVPINVLLFLGAAMAISKVGSVTGMNQW
                     IAETVLPSYVPSNPYLLAIIIASISIILHMILGSVVAVLGIAVPALLIFTLPIGINPV
                     VTTLLV"
     gene            complement(1223..2467)
                     /locus_tag="PLD67_05840"
     CDS             complement(1223..2467)
                     /locus_tag="PLD67_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HOT22075.1"
                     /translation="MKTEFFINNRAKFAEKMDDYSTAVFFSGKAPRESADQQYSFSVD
                     RNFFYLTGIDREDMVLIISKIAGKVDETLYIPPVDEHFEKWYGILMRKEEAINVSGVK
                     KVEDRNDFLKQFAAKLSSADRPDNVYIFSYITDVEEPYDCYRNFAHWMRNQYPAVNIK
                     NSLDIMIDLRSSKSEEEVEEIKTAISYTKDALEFVMKNLKPGKFEYQVRADFEYQLML
                     RNSRPSFHIIGASSGNAVILHYVDLQRKMEDGDMILLDLGALSNNYASDITRTYPVNG
                     KFTQRQKDIYNVVLGAQDVAIDAMHVGVSETDVNNKVKAYFAKELKTLKLIKDDSEVD
                     KYYYHGVGHPLGLDVHDLRRRDKTMQENNVYTVEPGLYIKEEGIGIRIEDDVWVTKNG
                     IINLSKDIIKTVNDIENFMNSR"
     gene            complement(2595..>3417)
                     /locus_tag="PLD67_05845"
     CDS             complement(2595..>3417)
                     /locus_tag="PLD67_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HOT22076.1"
                     /translation="EIYANTNAEIFGAEIPISGIAGDQQAALFGQLCFQEGMVKNTYG
                     TGCFMLMNTGEKIVQSHKGLITTIAWGLQGKVEYALEGSVFVAGAAIQWLRDELKIIH
                     EAADSEYFANKVKDTEGVYVVPAFTGLGAPYWDMYARGAIVGLTRGAGRNHIIRATLE
                     SIAYQTKDIIEAMIEDSGINLTVLKVDGGASANNFLMQFQSDILNVNIERPEITETTA
                     LGAAYLAGLATGFWKSKGEVIQNWSMNKKFKPSINDDERNRLYSGWKKAVKKAMS"
BASE COUNT         1035 a          721 c          509 g         1152 t
ORIGIN      
        1 gaccaataag gttgttacaa cgggatttat tccaataggc aaagtaaata ttaacaaagc
       61 aggcactgca atgcctaata cagcaaccac gcttcccaat atcatatgaa gtattattga
      121 tattgatgca ataattatag ccaacaaata ggggttagat ggtacatatg aaggaagaac
      181 tgtttccgca atccattgat tcattcccgt aactgaccct accttgctga ttgccatggc
      241 agctcctaag aataataata cattaatggg aacttcctgc cactgctttg gagacagcac
      301 tgcaccaata acaggaaagc tcattagcat ggcaattaaa aaagtaatcc atccgatttc
      361 aataccatga ataaaatcag tcatccataa aactacagct ataataagcc agacaacggt
      421 acgtttttct atcttggata atgctcctaa tgttattaat ttatttttta tttcagcttt
      481 gtttattttt acttctgatg taggtttaaa caaaatgaga attaaagcac atgtaagtat
      541 acttgcaata atattgggag gtcccataaa aataaaccac tgaatatagc ttatggattc
      601 tcctgatgct tgaactgtca atggatttat aacactgtcc cctgttaaaa atatcattga
      661 tattggaact gctgctgcaa aaacagcaaa tccgatttta gacttgtccc cacttgctat
      721 atttgcactt ttaataacca catccattac cgacataatt ataaatgccc tcggccaagg
      781 atgaggaatt ataatactta atataaatgt aagcataaat atggaatata ttatactttt
      841 ataggagtgc acaaatttaa taataaatgc atatgcaatt ctttctccca agcctgataa
      901 ctttactgct gaagctatca gatatgctcc gattaccaaa taaattgtac tgcttaaaaa
      961 gggagaaaaa acaactgatg gctcagccac cttaaaaatt atcaaaagta ctaagtaaag
     1021 acctgaagca tatcctgatt gaactgtatg gaaagtccaa aataaaacag tcatcaaggt
     1081 taatccaaga gacattttcc cttggggaga aatgcctctt aatggaagaa gatttattgt
     1141 aatcgctgct aagattccaa aaataaaccc gactattctt ttgtttttag tcactgatac
     1201 acctcattta agttaattaa gtctatctgc tattcatgaa attttctata tcatttactg
     1261 tcttaataat atccttcgat aaattaatga tgccgttttt tgttacccaa acatcatctt
     1321 caattctaat acctattcct tcttctttta tataaaggcc aggctcaacg gtgtacacat
     1381 tgttttcctg catcgttttg tctcttcttc ttaaatcatg aacgtccaat cccaatggat
     1441 ggcctactcc atgataatag tatttgtcaa cctcagaatc atcctttatt aatttaagcg
     1501 tttttaattc ctttgcaaag taagctttaa ctttattgtt tacatctgtt tctgagactc
     1561 ccacatgcat tgcatcgatc gcaacatcct gcgctcccaa aacaacatta taaatatcct
     1621 tttgcctttg tgtaaactta ccgtttacgg gatatgtcct tgtaatatcg gaggcataat
     1681 tgtttgacaa ggcaccaagg tccaaaagta tcatatcgcc gtcttccatt ttacgttgca
     1741 aatcaacata gtgtaatatt accgcattac ctgatgaagc accgattata tgaaagcttg
     1801 gtcttgaatt tctaagcata agctgatatt caaaatctgc tctgacttga tattcaaact
     1861 ttccgggctt taaattcttc attacaaatt ctaaggcatc tttcgtataa cttatggcag
     1921 tttttatttc ttcaacttct tcttctgact tagaacttct taaatcaatc attatatcca
     1981 gcgaattctt tatatttact gcaggatatt gatttctcat ccaatgagca aaatttctgt
     2041 aacagtcata aggctcttct acatctgtaa tatatgaaaa aatatacaca ttatcaggcc
     2101 tgtctgcaga tgataattta gcggcaaact gtttaagaaa atcatttctg tcttcaactt
     2161 tttttacacc tgagacattg attgcttctt cttttctcat gaggataccg taccatttct
     2221 cgaaatgctc atctacaggc ggtatgtaca acgtttcatc tactttgcct gcaattttgc
     2281 tgattattaa aaccatatcc tccctgtcta ttccggttaa ataaaagaaa tttctgtcta
     2341 cagaaaatga atattgctgg tcagcacttt ctctcggtgc ctttccggaa aaaaatactg
     2401 ctgtagaata atcatccatt ttttctgcaa attttgctct gttatttata aaaaattctg
     2461 tcttcatctt ttcctccatt tgatattata ctgtcgtctc cacgtatgac aaggggacgg
     2521 gggtactgtc atcgattgac cagaaagaac cgtccccgat ggactcgatg gactatgttc
     2581 ttgatgacaa acgtctaact cattgccttt tttactgcct tcttccaacc actatataat
     2641 ctatttctct cgtcgtcatt aattgacggt ttaaactttt tgttcatgct ccagttttgt
     2701 ataacttccc cttttgattt ccaaaaccct gttgcaaggc ctgcaagata tgcggctcca
     2761 agtgcggtag tttctgtaat ctcaggtctt tcaatattta cattaagtat atctgactga
     2821 aattgcatta aaaaattatt tgccgatgct cctccgtcaa cctttaatac cgttaaattg
     2881 attcctgaat cttcaatcat tgcttcaatt atatccttag tttgataagc aattgattca
     2941 agagttgccc ttattatatg atttctgcct gctccccttg tcaaccctac aattgctccc
     3001 cttgcataca tatcccaata aggggcacca agtcccgtaa atgcaggcac aacatagaca
     3061 ccttctgtat ccttgacctt atttgcaaaa tattcggaat ctgctgcttc atgtattatt
     3121 ttaagctcat ctctaagcca ctgtattgct gcacctgcca caaatacaga gccttccaag
     3181 gcatattcga ccttcccttg taagccccat gcgattgtag taatgagccc cttgtgagat
     3241 tgaactattt tttcacctgt attcataagc ataaagcatc ccgtaccata tgtattcttt
     3301 accatgcctt cctgaaaaca gagctgacca aagagagcag cttgctggtc tcccgcaatt
     3361 cctgatattg gtatttcagc accgaaaatt tcagcattgg tattagcgta tatttct
//
LOCUS       DAOSOW010000195         3403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_27934_length_3403_cov_4.119176, whole genome shotgun sequence.
ACCESSION   DAOSOW010000195 DAOSOW010000000
VERSION     DAOSOW010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27934_length_3403_cov_4.119176"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="PLD67_05850"
     CDS             <1..483
                     /locus_tag="PLD67_05850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOT22077.1"
                     /translation="KNKMETIIQQNVELGIKSFIPLITERTVVKLNEKDREQKKLDRW
                     QKIAKESAKQSKRNIIPTVEPIITVSELIEKLKHIDAEIIVPYELEGVKILKDVLKEP
                     KHNYYIVIGPEGGFDIKEIEMFQEIGAHIVTLGKRIIRTETAGIVTASVIMYACNEMI
                     "
     gene            733..2013
                     /locus_tag="PLD67_05855"
     CDS             733..2013
                     /locus_tag="PLD67_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013360823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOT22078.1"
                     /translation="MAKKNLGLAQKVLIGLAIGFVVGLIVHYMPKGTLRDDVLIDGVF
                     QFVGQVFLRGIMMCVVPLVFVSLVNGAASMGDIKKLGKVGGKTMAFYLVTTAVAIVIG
                     LVMAFVISPGIGLDMGAIDTVDVTASEGKPIAQVLYEMVPRNPIQALAEGNMLQIIVF
                     AIITGIALTMIGEKGKKVLELFEELNELIMKLVSIVMVLAPFGVLCLVARTFGQVGVA
                     AVFPLAKSVAAVYIALALHLVLVYGGMLKLLSGLAPVRFFKKIFPAMAVAFSTASSSA
                     TLPVTMDLTVNKIGASKDISAFALPLGATVNMDGTAIYQGVSAVFIAQVYGINVTPAM
                     ALMIVLSATLASIGTAGVPGAGAIMLSMVLQTVGLPLEGLGLILGIDRLFDMGRTAMN
                     ITGDAVCTTIISKQEGEFNEEIWNTDFAKEETAK"
     gene            2251..3378
                     /gene="hemW"
                     /locus_tag="PLD67_05860"
     CDS             2251..3378
                     /gene="hemW"
                     /locus_tag="PLD67_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOT22079.1"
                     /translation="MKKLGIYIHIPFCVKKCGYCDFYSVKWDEESENKYIHSAINEIK
                     SYNELSSKYVVDSIYIGGGTPSIINPKNLEKIISTIRCLFTVEENSEISMEANPNSLW
                     ENIKTYGEIGINRLSIGIQSLNDNILKRIGRIHNSKEALQAIDRAISFGFENINADVM
                     FNIPGQTVDDINDTISKLVTKQIRHISFYSLKLEEGTPMYLLKKNKKIVMPEEDLERE
                     MYYAGRNIMEKHGLMQYEISNFSVKGYECRHNLKYWKQEEYIGIGPSAHSFLGNVRFS
                     NPSDLTEYITSGENGVFERNTLEEMDENDIKFEYIMLRLRLTEGLKFADFKNKFSIDF
                     KEAYKEQIKHLTENNLIESDDDAIRLTKRGMDLSNYVFSQF"
BASE COUNT         1220 a          488 c          698 g          997 t
ORIGIN      
        1 aaaaataaaa tggaaacaat catacagcaa aatgtagagt tgggtattaa aagctttatt
       61 cctttaataa cagaaagaac cgttgttaaa ctcaatgaaa aggacagaga gcaaaagaag
      121 ttggacagat ggcaaaaaat cgccaaagaa tcagctaaac agagcaaaag aaatatcatt
      181 cccacagtcg aaccgataat tactgtcagt gagcttattg aaaaacttaa gcatatagat
      241 gcggaaataa ttgtacctta tgaattagag ggtgtaaaaa tattaaagga tgttttaaaa
      301 gagccgaagc ataattatta catagtaata ggccctgagg gaggttttga tattaaagaa
      361 attgaaatgt ttcaggaaat cggcgctcat atcgtaacct taggcaaaag aattataagg
      421 actgaaactg ccggtatcgt aacagcttct gtcataatgt atgcttgtaa cgagatgata
      481 tagcaggaca aaagttttcc ggaaaattca tgggaatttt ccggtttttt tattttataa
      541 aagaaacaaa aggaataaaa catactatta tctaatacgt ttattataaa aggaggtttt
      601 gtataaattg taaatatttg ttggacaaaa ttcatatgat tagattgaca aactattcag
      661 gaatatatac aatgtaaacg taaacagttg attgtttata aagtttttta aatttttaaa
      721 aggaggatta taatggcaaa gaaaaatctg ggattagcac agaaagtcct tatagggtta
      781 gccattggtt ttgtcgtagg tcttatagtt cattatatgc caaaaggcac acttagggat
      841 gatgttttaa ttgacggtgt attccaattt gttggtcagg tattcctaag aggaataatg
      901 atgtgcgtag ttcctttggt atttgtttcc ttagtaaatg gtgctgccag catgggcgac
      961 atcaagaaac tgggtaaagt cggcggtaaa acaatggcgt tttacttagt gactacagct
     1021 gtagcaatag taataggtct tgtcatggcc ttcgtaataa gtccgggtat cggactggat
     1081 atgggtgcta ttgacactgt agatgttact gcaagtgaag gtaaaccaat agcacaagtt
     1141 ctttatgaaa tggtacccag aaacccgatt caggcattag ctgaaggaaa tatgcttcaa
     1201 attattgtat ttgcaataat aaccggtata gcattgacca tgattggtga aaagggtaaa
     1261 aaagtacttg aattatttga agaattaaat gaattaataa tgaaattagt aagtattgta
     1321 atggttttag ctccattcgg agtgttatgt ctggtagcca gaacctttgg gcaagtagga
     1381 gttgccgctg tcttcccgtt ggcaaaatct gttgctgcag tttatattgc attagcatta
     1441 catttagtac ttgtatatgg cggaatgcta aaattgttat cgggattggc accggttaga
     1501 ttctttaaga aaattttccc tgcaatggca gttgcattct caacagccag cagcagtgct
     1561 acattaccgg taacaatgga tttgactgtt aataaaatag gtgcttctaa ggatatatca
     1621 gcgtttgctc ttccgctcgg tgcaaccgtt aatatggacg gaactgctat atatcaagga
     1681 gtttcagctg tatttattgc acaggtatat ggaattaatg taacacctgc tatggcactt
     1741 atgattgtgc tatcagctac attggcatca atcggtactg caggagttcc gggtgccgga
     1801 gcaataatgc tttccatggt attgcagaca gtaggattgc ctcttgaagg attaggttta
     1861 atactgggta ttgacagact attcgacatg ggtagaactg ccatgaacat tacaggtgat
     1921 gctgtatgta ctacaataat ctctaaacaa gaaggagaat ttaacgaaga aatttggaat
     1981 actgatttcg caaaagaaga aacagctaaa tagtcagact atgtgggaca gtgggattga
     2041 accgactgtc cctttttttt gtcatcctga gcgaagcgaa ggatctcatg agattcttcg
     2101 agctaaagct ctcagaatga cacaaaggca agtcatgata aaatggtaaa acctcagaat
     2161 tatcacgtgt taatacacca cgataaacga tatgttctgt gattgtcatc ctgagcgaag
     2221 cgaaggatct cataaaaaac agaggtagtt atgaagaaac taggaattta tatacatatt
     2281 ccattttgcg taaaaaagtg cggttactgc gacttttatt cggtaaaatg ggatgaagaa
     2341 tcagaaaata aatatattca ttcagcaatt aacgaaataa aaagctacaa tgaattgtca
     2401 agtaaatatg tggttgacag tatatatatc ggaggaggaa ctccttcaat tataaatccg
     2461 aaaaatcttg aaaaaatcat aagcaccata agatgtttat ttaccgtaga agaaaattcc
     2521 gaaataagca tggaagcaaa ccctaactca ttatgggaaa atataaaaac ttacggagaa
     2581 atcgggatta acagattgag tatcggaatt caatcattaa atgataatat attaaaaaga
     2641 ataggcagga ttcacaattc aaaagaagct ctgcaagcaa ttgacagagc tattagtttt
     2701 ggatttgaga atattaatgc agatgttatg tttaacattc ccggacagac cgtcgatgat
     2761 ataaatgata ccatttctaa attagtaaca aaacaaatca ggcatatatc cttttattca
     2821 ctgaaattag aagaaggaac accaatgtat ttgttgaaaa aaaacaaaaa aattgttatg
     2881 cctgaagaag accttgaaag ggaaatgtat tatgcaggca gaaatattat ggaaaagcat
     2941 ggtttaatgc agtacgaaat ttcaaatttt tccgtgaaag gatatgagtg caggcataat
     3001 ttgaagtatt ggaaacaaga agagtatata ggaatcggac cttcggctca ttcattttta
     3061 ggaaacgtaa gattcagcaa tccttcagac ctcacagaat atataacaag cggtgaaaac
     3121 ggagtttttg aaagaaacac tcttgaagaa atggatgaaa atgatattaa atttgaatat
     3181 ataatgctgc gtttaaggct aacggaggga cttaaatttg cagattttaa aaataaattt
     3241 tcaattgatt ttaaagaagc atataaagag caaataaaac atcttacaga aaacaactta
     3301 atcgaatcgg atgatgatgc cattcgcctt actaaaaggg gaatggacct atcgaattat
     3361 gtattttctc agttttaata cacttattgt catcctgagc gat
//
LOCUS       DAOSOW010000196         3402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_27952_length_3402_cov_2.674335, whole genome shotgun sequence.
ACCESSION   DAOSOW010000196 DAOSOW010000000
VERSION     DAOSOW010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27952_length_3402_cov_2.674335"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="PLD67_05865"
     CDS             <1..963
                     /locus_tag="PLD67_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004852942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HOT22080.1"
                     /translation="DYHPMYKNLGVPAFNEVKFSITSTRGCFGGCSFCSLNFHQGRII
                     QARSHESILNEVDKMKKDKDFKGYIHDVGGPTANFRIKACKKQEKYGACKNKECLTPQ
                     KCENLQIDHKDYIELLKKLRSIEGIKKVFIRSGIRYDYAIYDNNDDFIEELCKYHVSG
                     QLRTAPEHVSESVLKLMGKPSIKIYNKFVEKFNDVNKKIKKEQYIVPYFISSHPGSRL
                     KDAIELAEYIRDMGHIPEQVQDFYPTPGTLSTCMYYTGFNPLTNKEVYVPKDIEEKKM
                     QRALLQYSKKENYELVLKALKKENRYDLIGYGPKALIKPRQKKH"
     gene            1097..2362
                     /locus_tag="PLD67_05870"
     CDS             1097..2362
                     /locus_tag="PLD67_05870"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOT22081.1"
                     /translation="MNRKVRRRKKRIKLMSWSLVLLVGVLAASFVVRGIGKDGKKDKQ
                     PLEEHPEEQIGEEASNEVKEPQPEPDVTVRIRATGDIMFHPSQLDGAYDYKTNTYNFE
                     HSFKAIKSIIREADIAIANFEGTTAGNEVYAYQGYPLFNAPDEALDAIKNAGFDILST
                     ANNHSLDTRKAGIIRTIEQIKARGMEPIGTFTSKPETRVLIKDVKGIRFAFMAYTEMV
                     NGLETVLTPEDLDVMVNIIDEEKMKEDIIYAKDNNADVIIAYLHWGDEYSRIQAQRQE
                     ILADMLFRSGVDIILGSHPHVIQPTQTLDYDGKTKFIAYSMGNFLSNQRVETLVPYGM
                     SEQVSKYTEDGIIIDIEIEKKGSTKEISIKNINYIPLWVYKGVKENGTAEHIVYPIMD
                     YIESAELDEDSKARMSRSYADTMAQMNAE"
     gene            2377..3216
                     /gene="cdaA"
                     /locus_tag="PLD67_05875"
     CDS             2377..3216
                     /gene="cdaA"
                     /locus_tag="PLD67_05875"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOT22082.1"
                     /translation="MYDIMHRMGLIMSNIGPNTIVDIGIVYYVLYRGYILIRDMRAKQ
                     LVKGIVLLVALIPISQIFELYMVEFILSNTFQVGLIALVIVFQPEIRKALEYLGRTSF
                     TLSNVEKTNETSRKIIKEIISAITSLSRQKIGALIIFEKQIGLNDIIESGTVINANIS
                     SGLLINIFIPNTPLHDGAVIIKDYTIKAAGCFLPLTENNLLSKDIGTRHRAAIGMTER
                     SDSIALIVSEETGYISYAVEGRLYRNIQNEEIESLLQSIYIENEKVNIIDKWRNRNED
                     KKR"
     gene            3197..>3402
                     /locus_tag="PLD67_05880"
     CDS             3197..>3402
                     /locus_tag="PLD67_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22083.1"
                     /translation="MKIKNDKLVIQITCLIVAFVLWMIVMLVTNQTLEQSYPNVSVTI
                     RNLSALEKSNLVIMNQDKENITVTV"
BASE COUNT         1288 a          490 c          669 g          955 t
ORIGIN      
        1 gattaccacc ctatgtataa aaacttggga gtacctgcat ttaatgaagt aaaattcagc
       61 attacaagta caagaggatg tttcggaggc tgcagctttt gctcccttaa tttccatcag
      121 ggaagaataa tacaagcaag aagtcatgag tccatattaa atgaagttga taagatgaag
      181 aaagacaagg actttaaagg atatattcat gatgtggggg gacctactgc taatttcaga
      241 attaaggctt gcaaaaaaca ggaaaagtac ggtgcgtgca aaaataaaga atgtttaact
      301 ccgcaaaaat gcgaaaattt acagatcgac cataaagatt atatagaatt attgaagaag
      361 ctgagaagta tcgaaggtat caaaaaagtt tttattcgtt caggcataag atacgattat
      421 gcaatatatg ataataatga tgactttatt gaagaattat gcaaatacca tgtgagcgga
      481 caattaagaa ccgctccgga acacgtttca gaatcagttt taaaactaat gggcaaacct
      541 tcaataaaaa tatacaataa gtttgtagaa aagtttaatg atgtaaacaa aaagattaaa
      601 aaagagcaat acattgtacc ttattttata tcatcgcatc ccggttctcg cttgaaagat
      661 gcaattgagc ttgctgaata tataagggat atgggtcata ttcccgagca ggtacaggat
      721 ttttatccta caccggggac actgtcaaca tgcatgtatt atacgggctt taacccattg
      781 acaaataaag aagtttatgt tccaaaagat atagaagaaa agaaaatgca aagagcattg
      841 cttcaatatt ccaaaaaaga aaattatgag ctggttttga aagctttaaa aaaggaaaac
      901 agatatgatt taatcggata cggtcccaaa gctttaataa agccaagaca aaaaaagcac
      961 taaaggtgcc tggcaccggg taagggtgcc tggcaccaac atccggtatc tttaatttaa
     1021 ttttaataat ttaatttctt gcaaaatctt tcatagtagg atagaataaa tacgtaatta
     1081 ttcatagagg tagcatatga acagaaaagt cagacgacgt aaaaaaagaa ttaaattaat
     1141 gtcatggtca ttagtattat tggttggtgt attagcagct tcttttgttg taaggggtat
     1201 aggtaaagac gggaaaaaag acaagcagcc gctggaggag catccggaag aacaaatcgg
     1261 agaagaagcc tctaatgaag ttaaggagcc tcagcctgaa cctgatgtga ctgttagaat
     1321 aagagcgaca ggagacataa tgtttcatcc ctcacaattg gatggagcat atgattacaa
     1381 aacgaatacc tataattttg aacacagttt taaagctata aaaagtatta tccgagaagc
     1441 ggacatagca atcgctaatt ttgaaggaac aacagcggga aatgaagtat acgcatatca
     1501 gggatatccc ttgtttaatg ctcccgatga agcattggac gctataaaaa atgcgggatt
     1561 tgacatactg tcaactgcca acaaccacag ccttgataca agaaaggcag gtattataag
     1621 aacaatagag caaattaaag caagaggaat ggagccaatc ggaaccttta cttcaaagcc
     1681 tgaaacaagg gtattaatta aggatgtaaa gggtataagg tttgcattta tggcatatac
     1741 ggaaatggta aacggtcttg aaacggttct tactcctgaa gacttggatg taatggtaaa
     1801 tattatagac gaagaaaaaa tgaaggaaga cataatctat gccaaagata ataatgccga
     1861 tgtaataata gcataccttc attggggaga cgaatattca agaattcaag ctcaaaggca
     1921 ggaaattttg gcagacatgc ttttcaggtc aggagttgac ataatactcg gaagccatcc
     1981 tcatgttata caacctacgc aaacattgga ttatgacggt aaaacaaaat ttatagcata
     2041 ttccatgggt aattttttat caaatcaaag agtggaaaca ttagttcctt acggtatgtc
     2101 ggagcaagtt tcaaaatata ccgaagatgg aattattatt gatatagaaa tagaaaaaaa
     2161 gggcagtaca aaagaaattt ccattaaaaa tattaattat attccgttat gggtttataa
     2221 aggtgttaaa gaaaacggta cagcagagca tattgtttat cccattatgg attatattga
     2281 aagtgcagaa ttggatgaag attctaaagc aagaatgagc aggtcatatg ccgatacaat
     2341 ggcgcaaatg aatgctgaat agtgcggggg attattatgt atgatattat gcatcgaatg
     2401 ggattaatta tgtcaaatat aggtccaaat accatagttg atatcggtat tgtttactat
     2461 gttctttatc gaggttatat tctaatcaga gatatgagag caaaacaatt ggttaagggt
     2521 attgtactac tggttgcatt aattcctata tcgcaaatat ttgaattgta tatggttgaa
     2581 tttatattaa gcaatacatt tcaagtcgga ttaatcgcgc ttgttattgt ttttcaacct
     2641 gaaatcagaa aagctcttga atatttggga agaacttctt ttaccctgtc aaatgtagaa
     2701 aagactaatg aaacttccag aaaaattatt aaagaaatta tatctgcaat aacgtctctt
     2761 tcaagacaaa aaatcggtgc actaattatt tttgaaaaac agataggtct caatgatata
     2821 atagaaagcg ggactgtaat aaatgccaat atttcaagcg gactattaat aaatattttt
     2881 attcctaata ctccccttca cgacggagca gttattataa aggattatac aataaaagca
     2941 gcaggatgtt ttttgcctct taccgaaaac aaccttctca gtaaagatat agggacaagg
     3001 catagggcgg caataggaat gactgaacgg tcagattcca ttgctttgat agtttctgaa
     3061 gaaacagggt acatttcata tgctgtggaa ggaagactat acaggaatat tcaaaatgaa
     3121 gaaattgaat cattattaca gagtatttat attgaaaatg aaaaggtaaa tattatagat
     3181 aagtggagaa acagaaatga agataaaaaa cgataaatta gtcattcaaa ttacctgtct
     3241 tattgttgca tttgttctgt ggatgattgt aatgcttgta actaatcaaa cacttgaaca
     3301 gtcttatcca aatgtgtctg taacaataag aaacttatct gccctggaaa aatctaattt
     3361 agtaataatg aatcaagaca aggaaaatat aaccgtaaca gt
//
LOCUS       DAOSOW010000197         3400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_27971_length_3400_cov_3.223318, whole genome shotgun sequence.
ACCESSION   DAOSOW010000197 DAOSOW010000000
VERSION     DAOSOW010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27971_length_3400_cov_3.223318"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /gene="rlmD"
                     /locus_tag="PLD67_05885"
     CDS             complement(<1..1104)
                     /gene="rlmD"
                     /locus_tag="PLD67_05885"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOT22084.1"
                     /translation="MLETGLVREVEIIDMNHTGQGLAKINNFVVFISGAITGDIVKIK
                     ITDKKKNYALAEIISIIKPSEFRIDSSCPHFQKCGGCQLMHMDYKEQLKFKRNRVINE
                     FRRALVNFDEEAVTNTIGMKEPFRYRNKTAFSVAKIEGEIHIGPYEQGTYNTVDIDGC
                     LIQSKAADEAVKAFKSLMEKYNIEAYDKKTKKGIIRNTIIRNNRKNEIMLIIVTRTEN
                     FKNKELLVSELSLISNIKTIIQNINNKNTNLIMGRKNITLYGSGTIIDTIDDLVFTIS
                     PETFFQINPVQTEKLYQTAIEYADIDKDSICFDIYCGIGTISLMAAKKAKYVYGVEIV
                     EQSIINARENANKNNISNVEFYTGKAEEILPKLY"
     gene            complement(1116..1601)
                     /locus_tag="PLD67_05890"
     CDS             complement(1116..1601)
                     /locus_tag="PLD67_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOT22085.1"
                     /translation="MFREMRRIKQLLSMEDTNAVMDRGTCGVMACLGDDDYPYAVPVN
                     YVYYNDKIYFHSSKDGHKIDAIKKHPKVSFAVVDKDTIVSEEFTSYFRSVIVFGKARI
                     AEGEEWKEAFQALVEKYSGNMPKDDKHKIVTECTRSHIIAIDIDHMTGKEAIELVNAK
                     K"
     gene            complement(1620..2414)
                     /locus_tag="PLD67_05895"
     CDS             complement(1620..2414)
                     /locus_tag="PLD67_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOT22086.1"
                     /translation="MKVFLSVDIEGITGVTSWNETNLGNQEHRLCAEQMTKETLAACE
                     GAITMGVKEILIKDAHDSARNIDFTKIPKCAKISRGWTNTPESMVAGLDETFDAAIFI
                     GYHSGAGYDGNPLSHTMSLSNNYIKINGEIASEFVINSYLAAKYGVPVVFLSGDKMLC
                     ETAKKFNKGIETLAVKEGVGGASISINPELSCELIREGVKKALKHTSACKIEIPDKFE
                     VEINYREHFKAKKASYFPGVSQTGPYTVKYTAKDINEFSTTMMFIL"
     gene            complement(2458..3396)
                     /locus_tag="PLD67_05900"
     CDS             complement(2458..3396)
                     /locus_tag="PLD67_05900"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOT22087.1"
                     /translation="MLRASQPQIHAVCRRITGNDADALDATQEALIAVVRGLGRFDGT
                     LNLIFQPAEETIGGAERMIREGCIEEPKVDYVLGLHVQPYLNAGKVELKYGKLNASTD
                     SINIVLKGKAAHAAYPDKGIDAIVIAGHVITALQSIVSRNTSPLNPVAISLGRISGGV
                     KDNIIADEVKITGTLRTLDDETRQYTKDRISSIVKNTARAFGGEGLTTFCEGYKALIN
                     NNEVVDIIKENAIKLLGKENVEFKEFPSLGAEDFSYFMDVAKGAFFHLGCGNPAKGIT
                     SPIHTEHFDIDEECLKVGVRLQVENVMTLLKRHENM"
BASE COUNT          847 a          728 c          550 g         1275 t
ORIGIN      
        1 atatagttta gggagtattt cttctgcttt tccggtataa aattcaacat ttgatatatt
       61 gtttttattg gcattttctc ttgcgtttat aattgattgc tcaacaattt caactccata
      121 tacatatttt gctttttttg cagccattaa agatatggtt ccgatgccgc aataaatatc
      181 aaaacaaata ctgtctttat caatatctgc atattcaatt gctgtttgat agagcttttc
      241 cgtctgaacg ggattaattt gaaaaaatgt ttcgggtgaa atggtgaaaa ctaaatcgtc
      301 aatagtatca attattgtac ctgacccgta caaagtaata ttttttctcc ccattattaa
      361 atttgtgttt ttattgttaa tgttttgaat tattgtttta atgtttgata taagagataa
      421 ttcactaacc agtagctctt tgtttttaaa gttttcggtc cttgtaacta taatgagcat
      481 tatttcattt tttcgattat ttctgattat agtgtttctt ataattcctt tttttgtttt
      541 tttatcataa gcttcaatgt tgtacttttc catcaagctt ttaaaagctt ttactgcttc
      601 atcggcagct ttgctttgta ttaagcagcc gtcaatatca acggtattat atgtaccttg
      661 ttcataggga cctatatgaa tttcgccctc tatttttgca accgagaatg ctgttttatt
      721 tctgtatcta aatggctcct tcatgccaat ggtattagtt acagcctctt catcaaaatt
      781 taccagagcc cttctaaatt cgttgattac tctgtttcgt ttgaatttta actgctcctt
      841 gtaatccata tgcatgagct gacatcctcc gcatttttga aaatgcgggc atgatgaatc
      901 gattctgaat tccgaaggct taataatgct gattatctct gctaaggcat agtttttctt
      961 tttgtctgtt atttttattt tcactatatc gcctgttatt gcaccgctta taaacacgac
     1021 aaaattattt attttagcaa ggccttggcc tgtgtgattc atatcgataa tttcaacttc
     1081 tctgactaat cctgtttcta acataatttc tccggttact ttttggcatt gactaattcg
     1141 atagcttctt tgcctgtcat atggtcgata tctattgcaa taatgtggga ccttgtgcat
     1201 tctgttacta tcttatgttt atcatctttc ggcatatttc cggaatactt ttcaaccaaa
     1261 gcctgaaagg cttccttcca ctcttcgcct tccgcaattc ttgcttttcc aaatacaata
     1321 acacttctaa agtatgaagt gaattcttcg cttactattg tatctttatc tactaccgcg
     1381 aaggatacct ttggatgttt ttttatagca tctattttat gcccgtcctt tgaagagtga
     1441 aaataaattt tatcattgta ataaacatag ttaacaggta ctgcataagg gtaatcgtca
     1501 tctcctaagc atgccatcac cccgcatgta cctctgtcca ttacggcatt cgtgtcttcc
     1561 attgacagta attgtttgat tcttctcatt tctctgaaca tttttatcct ccctgatttt
     1621 tatagaataa acatcattgt tgttgaaaat tcatttatat ccttggccgt gtatttaacc
     1681 gtatagggtc ctgtttgtga aactccggga aaatatgatg ctttttttgc tttgaaatgc
     1741 tccctgtagt tgatttcaac ttcaaatttg tccggaatct caatcttgca cgccgaagta
     1801 tgcttgagag cttttttgac tccttccctt atcaattcgc aggataattc aggattgata
     1861 cttattgaag ctcctccgac tccctcttta accgcaaggg tttctattcc tttattaaat
     1921 ttttttgctg tttcgcaaag cattttatcc ccgcttaaga acaccaccgg tacgccgtat
     1981 tttgccgcta aatatgaatt aataacaaat tcggaagcta tttcaccatt aatctttata
     2041 taattgttgc ttaagctcat tgtatgagac agcggatttc cgtcataacc tgcccctgaa
     2101 tgatagccga taaatatagc tgcatcaaat gtttcatcta accccgcgac cattgattcg
     2161 ggcgtatttg tccagccccg gcttattttt gcacattttg gaattttggt aaaatctata
     2221 tttcttgccg agtcatgtgc atcctttatc aatatttctt taactcccat tgttattgca
     2281 ccttcgcatg cagctaatgt ttccttggtc atttgttctg cacataatct gtgctcttga
     2341 ttcccaagat ttgtttcatt ccatgaggtt accccggtga ttccttctat atccacactt
     2401 aaaaatactt tcatttctcc ctcctgtttt taatgagata cttcaattcg ttctgaatta
     2461 catgttctca tgcctcttca gcaaagtcat tacattttcc acctgcagcc ttacccctac
     2521 ctttaaacat tcttcatcaa tatcaaagtg ctctgtatgg ataggcgatg ttattccctt
     2581 tgcaggatta ccgcatccca ggtggaagaa agcacccttt gcaacatcca taaaatatga
     2641 aaaatcttct gctccaaggc ttggaaattc tttaaattca acattttctt ttcccagaag
     2701 ctttattgca ttttccttaa taatatcaac cacttcatta ttattaatca aagctttata
     2761 tccttcgcaa aatgttgtaa gcccttctcc gccaaaagct ctagctgtat tttttacaat
     2821 acttgaaatt ctatccttgg tatactgtct tgtttcatca tctaaggttc tcagtgtacc
     2881 ggtaattttg acttcgtcgg caattatatt atccttaaca ccgcctgaaa ttctgccgag
     2941 tgatattgct accggattta aaggagaagt atttctgctt actattgact gcagtgcagt
     3001 tataacatga cctgctatta ctatagcgtc tattcccttg tcgggatatg ctgcgtgagc
     3061 agcttttccc ttcaagacta tatttatgga atctgtggat gcatttagct tgccgtattt
     3121 aagttcaacc tttcccgcat ttaaataagg ctggacatgg agtcccaaaa cataatcaac
     3181 ctttggctct tctatgcagc cttcccgaat cattctttct gcaccgccta ttgtttcttc
     3241 cgccggctga aaaatcaggt tcagcgtgcc gtcgaagcgg cccaggccac gtaccaccgc
     3301 gatgagggcc tcttgcgtgg cgtcgagcgc gtcggcgtcg ttgccggtga tgcgacggca
     3361 cacggcgtgg atctgcggct ggctggcccg gagcagccgt
//
LOCUS       DAOSOW010000198         3392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_28058_length_3392_cov_2.876236, whole genome shotgun sequence.
ACCESSION   DAOSOW010000198 DAOSOW010000000
VERSION     DAOSOW010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28058_length_3392_cov_2.876236"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..662
                     /locus_tag="PLD67_05905"
     CDS             102..662
                     /locus_tag="PLD67_05905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOT22088.1"
                     /translation="MTKIGIILGSTKPGRNGEAVAKWVYNIAKQREDAQFELVDVKDF
                     DLPLYDEPYPAAMQKYTKEHTKKWSKKISEFDGYVFVTAEYNHGIPGALKNAIDFLNA
                     EWKNKSVGFVSYGSAGGSRAVEQLRLVAAELQMADVRAQVMLSLFTDFQDMSIFTPDP
                     RHEGEVHTMLDQVIAWADALKPLRQV"
     gene            902..2695
                     /locus_tag="PLD67_05910"
     CDS             902..2695
                     /locus_tag="PLD67_05910"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HOT22089.1"
                     /translation="MSRIKNFMNNIVYNLKETITRFPMTIVFLSSLSTVMFLIIEDYS
                     SLDIDLLSRYMFAGIFGAFLATTVRFMMERYENLKNSFLFYGLTILLTIGYFYFMTDD
                     KVDNKMLIHLLVISFALFAAYLYLPSSKNALNFGNVALAHFKSAFTAILYGIVLYVGI
                     AAIIGAIDILLYDVDYKSYAHAANIIFIFFTPLYYLSLLPKFNSRDENDDAKKEISFT
                     YPRFLDILVSYITIPLITAFTAVLIIYFIKILATGVWPVGQVGPMVLGYSAAGYFIYI
                     LSSNLNNKFSVLYRKLFPLILIPLVVMQMVSSYIRIDAYGITESRYYVVLFGIFSIVC
                     ALVLIFGKKKNPNTIVLLSAVFALMSIIPPVDAFTVSKNSQERRLTEILNRNKMIVDG
                     EIVKKTDLSTDDMFEITNISNYMYGMGYLDDMDWFPDKYADNYYGNFKNIYGFEQYYD
                     RGFPGQEETLYLYAILDENEKINIEDYDLFFRIYIQNKRGPGEDIGEFSLKGKNYSIK
                     QIPDEKGYITITVSDDTNTLIEIPMKEFIDGLFEKSNAPKGAMDKESLTIEKQNDKLK
                     VKLLIYDMNVDRNDPDDIYISANIYVFVSTQ"
     gene            complement(2864..>3392)
                     /locus_tag="PLD67_05915"
     CDS             complement(2864..>3392)
                     /locus_tag="PLD67_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22090.1"
                     /translation="ENPESAMLLFISEYYPPILKGLSISAIVAAIMSSFDSFLILATT
                     HLVYDVGSSLNKDVNEEKIKKLLPKVTIVIGILGLIIALFIQSLFDYLYMVFSIIGSS
                     LVPAIIAALYYSDKTTSKAVVASIVTGAVVPGALYLTYGYDVFLGDPVFLGIIASTLV
                     LIIGSIVTKDKIATK"
BASE COUNT         1146 a          503 c          642 g         1101 t
ORIGIN      
        1 catatattga gtttttttat tgtttaaatt gaattgcaat gggtaatata tattatagct
       61 gattaagaat ataaattata taatattaat ggaggatata aatgacaaaa ataggtataa
      121 tattaggaag tactaaaccg ggacgcaatg gtgaagccgt tgcaaaatgg gtttataata
      181 tagcaaaaca aagagaagat gcacaatttg aactggtgga tgtgaaggat ttcgatttgc
      241 cattgtatga tgaaccatat ccggcagcaa tgcagaaata cactaaggag catacaaaaa
      301 aatggtcgaa aaagataagt gaatttgatg gttatgtatt tgttacagcc gaatataacc
      361 acggtattcc gggagcatta aaaaatgcaa ttgacttttt aaatgcagag tggaagaata
      421 aatcagtcgg atttgtaagc tacggaagtg ccggaggttc gagagcagtt gaacaattaa
      481 ggctcgtcgc agcagaactt caaatggcgg atgtaagagc ccaggtaatg ttatcattat
      541 ttacggattt tcaggatatg agcattttta cacctgaccc gcgccatgag ggtgaagtgc
      601 atacaatgct ggaccaggtt attgcatggg ccgatgcttt gaaacccctg aggcaggtat
      661 gaagaactca gccctaagca ggatggaggc actcttgcag ctaacacacc ggccaatggc
      721 tggtgtttta tatagaaaga tccttgcttg cttagtatac tgctaagttt tgcaaggatc
      781 ttgttaatta tataaatgtc cacaggggac tgttcgtgga tgtggtatat attactttta
      841 taagtaaagg aacaatattt tataaaaatt cgtcataaat tatatagaaa gaggtgatag
      901 aatgagcagg attaaaaact ttatgaataa tattgtttac aacctgaaag aaacaattac
      961 aagatttcct atgactatag ttttcttatc ttcattgtct acggttatgt ttttgataat
     1021 tgaagattat tcatcgttgg atatagattt attatcgaga tatatgtttg caggtatttt
     1081 tggtgcgttt ttggcaacaa cggttcgatt tatgatggaa agatatgaga acttaaaaaa
     1141 ttcattttta ttttacggat taaccatttt acttacaatt ggttatttct atttcatgac
     1201 ggatgataag gttgacaaca aaatgcttat acacttgctg gtaatttcct ttgcattatt
     1261 tgcagcatat ctgtatttgc cgtcatcaaa aaatgcttta aattttggca atgtggcact
     1321 tgcgcatttc aaatcagcct ttacggctat tctctacggt atagttttat acgttggtat
     1381 tgcagcaata ataggtgcta tcgatatatt gctttatgac gtagattaca aatcttacgc
     1441 gcatgcagcc aatattatat ttattttctt tactccttta tattatcttt ccttattgcc
     1501 gaagtttaat tcaagggatg aaaatgatga tgcaaaaaag gaaatttcat ttacctaccc
     1561 aagatttttg gatatattgg tttcatatat aacaattccg cttattaccg catttacggc
     1621 agtcttgatt atatacttca ttaaaatact tgcaaccgga gtatggcctg ttggacaggt
     1681 cggacctatg gttttgggat attctgcagc cggttatttc atctacattt tgagctcaaa
     1741 tttgaataac aaattttctg tgctttacag aaaactgttt ccattaattc taataccctt
     1801 agttgtaatg cagatggttt cttcatatat tcgtattgat gcatacggca ttacagaatc
     1861 cagatattat gtggtgttat tcggaatatt ttcaatagta tgtgcattag tgctcatatt
     1921 cggtaaaaag aaaaatccaa atactattgt tctcttgtct gctgtctttg cactgatgtc
     1981 aataattcct ccggttgatg cgtttaccgt ttctaaaaac agccaggaaa gaagactcac
     2041 tgaaattctt aacagaaaca agatgatagt agacggtgaa attgttaaaa aaacagatct
     2101 ttctactgat gatatgtttg aaataactaa tatttcaaat tatatgtatg gaatgggata
     2161 tttggacgac atggactggt tcccggataa atatgccgat aattactatg gcaatttcaa
     2221 aaacatctac gggtttgagc agtattatga cagaggattt ccgggacagg aagagacact
     2281 ttatttatat gccatacttg atgaaaatga aaaaattaat attgaagatt atgatttatt
     2341 tttcagaatt tatattcaaa acaaaagagg acctggcgag gatataggtg aattttctct
     2401 gaaaggtaaa aattattcga ttaagcagat acctgacgaa aagggttata taactataac
     2461 cgtttcggat gataccaata cattgattga aataccgatg aaggaattta tagatgggct
     2521 ttttgaaaag tcaaatgctc ctaaaggggc aatggataag gaaagcctta caattgaaaa
     2581 gcaaaacgat aagttgaaag ttaaattgct tatatacgat atgaatgttg ataggaatga
     2641 tcctgatgat atatatattt ctgcaaatat ttatgtgttt gtatctactc aataacgaag
     2701 gaactcaagg gatttttcga gctaagtact cataatgaca gaaatgtcat tcaaagcaat
     2761 aataaaacca gaataaactt tatcataaaa aactgaaggt caagaactat aagttcttga
     2821 cctgttggtt tttaccttta catcagctct aaaagagtgt caattacttg gttgcaatct
     2881 tatctttagt tacaatagag ccaattatca agactaaggt agatgcaata attcctaaaa
     2941 atacagggtc tcccaagaat acatcatacc cgtaagtcaa gtagagtgca ccgggaacaa
     3001 cagcgcctgt aacaatactg gcaacaactg ctttgcttgt cgttttgtca ctgtaataaa
     3061 gcgcagctat tattgcaggt accagggacg agccaattat tgagaatacc atatacaaat
     3121 agtcaaataa tgattgtata aacagagcta taattaaacc aagaattcct attacaatag
     3181 taacctttgg aagaagcttt tttatttttt cttcatttac atccttattt aatgaactgc
     3241 ccacatcgta aacaagatgt gtagttgcaa gaattaagaa ggaatcaaat gatgacataa
     3301 ttgcagctac tattgctgaa attgacaatc cttttaaaat aggaggataa tattctgata
     3361 tgaataaaag cattgcagat tccggatttt ct
//
LOCUS       DAOSOW010000199         3388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_28090_length_3388_cov_3.440744, whole genome shotgun sequence.
ACCESSION   DAOSOW010000199 DAOSOW010000000
VERSION     DAOSOW010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28090_length_3388_cov_3.440744"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PLD67_05920"
     CDS             <1..317
                     /locus_tag="PLD67_05920"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOT22091.1"
                     /translation="KIPGKMVPGMGGAMDLVVGAKTVIIAMVHTQKGKPKILKQCTLP
                     LTAKEQVDIIVTEMALIRVTKDGLVLEELGPEATVEDVVDATEANLIISPNLKRFGVE
                     EE"
     gene            complement(455..1711)
                     /locus_tag="PLD67_05925"
     CDS             complement(455..1711)
                     /locus_tag="PLD67_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOT22092.1"
                     /translation="MDFENNFNDNLEKENQNNITNDSMSNEYGEINFVMNEPLIKKPK
                     KRRIKSVMGYIALVLVAAIIGGLTGSYVTANMLNAAAEKKAAESSINAQEVKINLTDD
                     VYFAVAVAEKSQKTVVGITTEVERQINTFWGPQKRTEQHMGSGFIVDSNGYIVTNSHV
                     IGDGNYKNITVSLIDGSIETGNVLWYDTNLDLAIVKINKTRLPAAELGNSDELMVGEP
                     AVAIGNPMTLDLERTVTQGIISGLNRSIQFEDGTVIEPLIQTDASINSGNSGGPLFNA
                     KGQVIGINTAKMTSAEGLGFAIPINIIKPVLTQVVTGGTFIPVYVGITGMDIETYEML
                     YGVDLMADYGVIIIETMKDSPAREAGLQPNDVIIEIDGKKTEYMVDIQKKLYEYQDGD
                     KSEFKILRNGEELKLVLTLRNRPDGI"
     gene            1937..2314
                     /locus_tag="PLD67_05930"
     CDS             1937..2314
                     /locus_tag="PLD67_05930"
                     /inference="COORDINATES: protein motif:HMM:NF015729.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HOT22093.1"
                     /translation="MKKIFKIVIQIAIIYAIYQAGNFISSLISNIIVIPGNIIGMVIL
                     LVLLITNVLKFSIIEETSNFMLKYMSFFFVPITVGIMESYKLIQDSIVQIIIILFISC
                     ILVMYVSCKVTDVLISYREKSHD"
     gene            2307..2984
                     /locus_tag="PLD67_05935"
     CDS             2307..2984
                     /locus_tag="PLD67_05935"
                     /inference="COORDINATES: protein motif:HMM:NF016088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HOT22094.1"
                     /translation="MIDILTALFFILLTIIVFKFSLLVNDKFKIVFLNPILLTAFIII
                     VILKIFNFTYSDYYKGGKILVSALGPIVVVLAIPLYKNRHDLVKNFIPIVGGIISGII
                     TTFVSITVLCKLFGIDEIIFLSLLSKSVTTPMAVESTKILGGNEAVTILAVIITGLIG
                     AAFAPIVINKGKIKNNIAKGIGIGSAAHALGTSKAIEMNEEAGAASGLAIGLTGLLTI
                     IAVIIFA"
     gene            2994..>3388
                     /locus_tag="PLD67_05940"
     CDS             2994..>3388
                     /locus_tag="PLD67_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT22095.1"
                     /translation="MINLSEIKKEDMDSIYKWFADTEFLKYYDYYPPNPLDKSDVDKM
                     FKYYEESGKSKVFAVRKESTIIGVAGFDDIIKENQVATLFIGLGSENERGKGYGTETM
                     RLLLKYGFENLNFHRIQLNVLEFNVGAIF"
BASE COUNT         1068 a          561 c          583 g         1176 t
ORIGIN      
        1 ggaaaatacc gggtaaaatg gttcctggca tgggcggagc aatggacctg gttgtaggtg
       61 caaaaacagt tattattgca atggttcata ctcaaaaggg gaaaccgaaa atattgaaac
      121 aatgtactct tcctttgact gctaaggagc aggttgatat catagttacc gaaatggctc
      181 ttatcagagt gacaaaagac ggcttggttc ttgaagaatt aggacccgaa gcaacagttg
      241 aagatgttgt tgatgcaaca gaagcaaatt taatcatttc accgaatttg aaacgttttg
      301 gcgtggaaga agaataatat aataaaaatt gtcattctga aggcgcagcc tgaagattct
      361 cacttttcaa taatgatgag agccttcgct tgcgctcaga atgacataaa gggatttgtt
      421 attaatatga aacaggagct gatctcctgt ttttttaaat gccgtcgggt ctgtttctta
      481 atgttaaaac gagttttagt tcttcaccgt ttcttaaaat tttaaattcc gatttatctc
      541 catcttgata ttcgtatagt tttttttgta tatcaaccat atattctgtc tttttcccgt
      601 caatttcaat aataacatca tttggctgaa gtccggcttc cctggcagga gaatctttca
      661 ttgtttctat tataattact ccatagtctg ccattaaatc gactccgtaa agcatttcat
      721 aagtttcaat gtccatgcct gtgatgccca catatacggg tataaaagtt cctcctgtaa
      781 ccacttgtgt caaaaccggt ttaattatat taatcggtat agcaaaaccc aatccttctg
      841 ctgaggtcat tttggccgta ttgattccta ttacctgtcc tttggcgtta aacaaggggc
      901 cgccgctgtt gcctgaattt atggaggcat ctgtctgaat aagcggctct atgacagtgc
      961 cgtcttcaaa ttggatagac ctgttaaggc cgcttataat accttgagta actgttcttt
     1021 ccaggtctaa ggtcattgga ttaccgattg caaccgcagg ctccccaacc attaattcgt
     1081 ccgaatttcc taattcggct gcgggaagtc ttgttttatt tatctttacg attgctaaat
     1141 ccaagttggt gtcataccac aatacattac cggtttctat tgagccgtca attaaagaaa
     1201 ctgtaatatt tttataatta ccatcaccta taacatgaga atttgttaca atgtatccgt
     1261 tcgaatctac aataaacccg gagcccatat gctgctctgt ccttttctgg ggaccccaaa
     1321 atgtattaat ttgtctttcc acctcggttg ttattcccac aactgttttt tgtgattttt
     1381 ctgcaactgc taccgcaaaa taaacatcat cggttaaatt aattttaacc tcttgagcat
     1441 taattgatga ctcagctgct tttttttcag ctgccgcgtt taacatattt gctgttacat
     1501 aactgcccgt tagacctcct attattgcag ctactaatac aagggcaatg taacccataa
     1561 cagactttat tcttcgcttt ttgggtttct taattaaagg ctcgttcatg acaaaattaa
     1621 tttcaccgta ttcatttgac atactatcat tagttatatt attttgattt tctttttcta
     1681 aattatcgtt aaaattattt tcgaagtcca ttaaacaccg ctccttattt attttaattt
     1741 tcgtttattc aatatagtat aattatatcc ttcttttctt tattgaaact taaattaata
     1801 aataaaataa atcgaaagaa ttactgttac tcccgcgaca gattatcatc acacaggtca
     1861 attcttaaaa atcaggcaat taaaaagata ttgaattatg tcagttttta tatataatgt
     1921 tatatacgga ggaaaaatga aaaaaatatt taaaatagtc attcaaatag caataattta
     1981 tgcaatatac caggcaggaa actttataag cagcctcata tctaatataa tagttatccc
     2041 cggcaatatt ataggcatgg tgatactttt ggtattattg ataaccaacg ttttaaagtt
     2101 ttcaataatt gaagaaacat ctaattttat gcttaaatac atgagttttt tctttgttcc
     2161 cataacggtt ggaattatgg aatcgtacaa actgatacaa gatagtattg tccaaataat
     2221 tataatttta tttatatctt gcatattagt tatgtacgta tcctgcaagg ttacggatgt
     2281 actgatttca tacagggaga aaagccatga ttgacatatt aacagctctt ttttttatat
     2341 tgcttacaat tattgttttt aaatttagcc ttcttgttaa cgataagttc aaaatagtat
     2401 ttttgaatcc gattctttta actgccttta ttataatagt tattttaaaa atatttaatt
     2461 ttacatattc tgattattac aaaggtggaa aaatattagt atctgcatta ggacctattg
     2521 ttgtagtgct tgcaataccc ctttataaaa acagacatga cttagtgaaa aattttattc
     2581 ctattgtagg aggaataata tccggcataa tcactacatt tgtatcgata acagtcttat
     2641 gtaaattgtt tggaattgat gaaataattt ttttatcact cttgtccaaa tcagttacaa
     2701 ctcccatggc tgttgaatca actaagatat tgggagggaa tgaagctgta actattttag
     2761 cagttataat taccgggtta ataggtgctg cttttgcacc tatagtaatt aacaaaggca
     2821 aaataaagaa taatattgca aaaggaatag gaatcggctc ggcagcccat gcattgggta
     2881 cttcaaaagc aattgaaatg aatgaggaag caggcgctgc aagcggtctt gcaataggct
     2941 taaccgggtt attgaccata atagccgtaa taatttttgc ataggagaca aatatgatta
     3001 atttatctga aattaaaaag gaagatatgg atagtatata caagtggttt gccgacactg
     3061 agtttttaaa atattatgat tattacccgc ctaatccttt agataagtct gatgtagata
     3121 aaatgtttaa atattatgaa gaaagcggaa aatcgaaagt gtttgccgtt agaaaagaaa
     3181 gcactattat tggagttgcc ggttttgatg atataataaa agaaaatcaa gttgcaactt
     3241 tgtttatcgg tttaggcagc gaaaatgaaa gaggcaaggg atacggcaca gaaactatga
     3301 ggcttttgtt gaagtatggt ttcgaaaact taaattttca cagaattcag cttaacgtgt
     3361 tagaatttaa tgtaggagct attttttt
//
LOCUS       DAOSOW010000200         3383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_28137_length_3383_cov_3.667668, whole genome shotgun sequence.
ACCESSION   DAOSOW010000200 DAOSOW010000000
VERSION     DAOSOW010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28137_length_3383_cov_3.667668"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(901..1704)
                     /locus_tag="PLD67_05945"
     CDS             complement(901..1704)
                     /locus_tag="PLD67_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT22096.1"
                     /translation="MFEQYFNLTKIPFTRDIPENELLNTVEIEELCGRLEYVARNRLF
                     AVVTGDVGSGKTTAIRKLVSNLDNNRYRVMYISDSALTPRNFYWEVLNQLGCEAKFYR
                     SDAKRQLTRELTNLIEIHKKIPVIIVDEAHLLTREMLEEVRFLLNSKMDSYNPMSLIL
                     VGQSELKDVLKKQIYEAICQRIDIRYHLSAYDRQQTGEYIKKHLAYAGETRDIFTDKA
                     VNEIYEYAHGVARKINKVCTASLMHAAQRQVKLIDDHMIRLIIEEEFSW"
     gene            complement(1697..>3005)
                     /locus_tag="PLD67_05950"
     CDS             complement(1697..>3005)
                     /locus_tag="PLD67_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOT22097.1"
                     /translation="ALKRHEMIAPLLIPELDAAEKRRIRHEILEREDISERTLRRYLA
                     AYREEGYEGLKPKERDDAGQLKAITEEVLDYAISLKQELPGRSVERIIHILEGEGIVG
                     KGEITRSTLSRHLRNRGFGAAELKLTKVNMLAARRFQKSGRNMLWQSDIKYGPYIPTE
                     KGGKKRTYMVAFIDDATRLVCHAEFYDNQRLPILEDCFRKAILKYGKPDAVYVDNGKV
                     FVSRWFRIACAKLGIKHMNTKAYSPESKGKIERFNKTVEAFLEELSLEKAKSLEELNR
                     KFRIWLDEGYNNREHSSLKGQSPMQAYTGDPRRVRFATPEECHDAFLWEDTRKVDNTG
                     CFKLNGIEYEAGIEYIGKKVDVRHDPFDMGHVEVWYSGEKRRTVEPLKIGEYCGRTEK
                     SGAVAKVSRSRLLKVYEKENDKRQKQRLGALSFRSMKGGEGNV"
     assembly_gap    3006..3015
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3127..>3383)
                     /locus_tag="PLD67_05955"
     CDS             complement(3127..>3383)
                     /locus_tag="PLD67_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22098.1"
                     /translation="EASVQTVRRWWAEYKDKLPQAVGALRSLLYRIFNKTVNELVMTG
                     VKGSALLGRVLEAFPPIDSGGLFMGEANMWLSSYWTGIML"
BASE COUNT          854 a          829 c          549 g         1141 t
ORIGIN      
        1 tttgcatgtc atttgaaaat gaatacaata cgcctctttg tgtgaattaa aatgtatata
       61 aataaatgaa tcccttcaca aatccctctg tcaatttatc agacaaaact tttaacagtc
      121 ccttattaaa tctagttgtt actttccccg ttcgcgttta taatacggtg atatgaataa
      181 gctgtagcgt ttttgctaag caataataat aactattagc aaagcaataa gggttgttca
      241 tatgcgaagt tggtaaatct tataattatt accttaaaaa taacatattt ggacaaatta
      301 ttctacagat ttatccaaat tattaataat cataaatatt tattagtttt atagcctttt
      361 attgttctgc aattaataat gagtctataa atggcattca ttatcataga aattgtgatc
      421 actttacatt tcattgcaaa taatcaatat agaatgtgtt aagtgattta ttgcacaaaa
      481 atctttcaga caagtgcgat attaaaaaaa gaaaattagc agcacctatg aatatgagta
      541 actcccatat aatttaaaga ttcgcatgct agcttagtca acatgaataa aagaaaagca
      601 attaattcaa acttaataaa tttcttactt aactgcttcc cgctccaaca gctccatcaa
      661 tatattaatc gcataaagcg gtatattggt taaccaggat tcctttctgt aatcagacat
      721 tgaggtgcga aaagcctgct tcggattgca tttttaacat aataaatcac aaaagtcaaa
      781 cgacttttgc gaataccatt gtcgctgctc ggtctgtttg accgcagctt gcttgcaatc
      841 catgaccgtc aattttgctc attaaccctg tccggtattg tcgccggaca gggtgtttaa
      901 ttaccagctg aattcttctt caataataag ccttatcata tggtcatcta tcagtttaac
      961 ctgcctttga gctgcatgca tcaggctggc tgtacatacc ttgtttatct ttctggctac
     1021 gccgtgagca tattcataaa tctcgttaac tgctttgtcg gtaaatatgt cccgggtttc
     1081 tcctgcatag gcaaggtgct tctttatgta ctctccggtt tgctgacggt cataggctga
     1141 caagtgatac cgaatatcta tacgctggca tattgcttca tatatctgct ttttcaatac
     1201 atccttcaac tcgctctgcc caaccagtat taaactcata gggttatacg aatccatttt
     1261 agagtttagc aggaatctca cttcttcaag catttccctc gttagcaaat gagcctcatc
     1321 tacaataatc accggtattt tcttgtgtat ctctatcagg tttgttaact cccgtgtcag
     1381 ctgccttttg gcatcgctcc gataaaactt agcttcacat ccaagctgat tcaatacttc
     1441 ccagtaaaag ttcctcggcg ttaatgctga atcactgata tacattactc tataccggtt
     1501 gttatccaaa ttactgacaa gtttcctgat tgctgtcgtt ttacctgatc ccacatctcc
     1561 tgttaccacg gcaaacaacc ggttccttgc tacgtactca agccttccgc atagttcctc
     1621 tatctcaacc gtattaagca gctcattctc tggtatatca cgggtaaagg gtatttttgt
     1681 gaggttgaaa tactgctcaa acatttcctt caccgccttt catactcctg aaggataagg
     1741 ctcccagtct ctgcttctgc cgtttatcat tctctttttc atataccttc aacagccttg
     1801 aacggctcac tttagcaacg gctccggatt tctctgttct gccacagtat tctccgatct
     1861 ttagcggttc tactgtcctt cgcttttctc cactgtacca tacttccaca tgacccatat
     1921 caaaggggtc atgcctcaca tctacctttt tcccgatata ttctatccct gcttcatact
     1981 ctattccatt gagcttaaag cagccggtat tatctacctt ccgtgtatcc tcccacaaga
     2041 atgcatcatg gcattcttcc ggtgttgcaa accgtactct tcttggatct cctgtatagg
     2101 cttgcatggg actttgccct ttaaggctgc tgtgctcccg attgttgtag ccttcatcca
     2161 gccatatcct gaatttccgg ttcagttcct caagactctt tgccttctca agtgacagct
     2221 cctccaggaa tgcttccact gttttattga accgttcaat tttccccttg ctttcaggtg
     2281 aatatgcctt tgtgttcata tgcttgattc caagctttgc acaggctatc ctgaaccacc
     2341 gggatacaaa cacttttccg ttgtctacat atacggcgtc aggcttccca tatttaagta
     2401 tcgcctttct aaagcaatct tccagaatag gcagccgttg attatcgtaa aactctgcat
     2461 gacataccaa tctggttgca tcatctatga aggctaccat gtaggttcgt ttctttccgc
     2521 ctttttctgt cggtatgtaa ggaccatact tgatatcact ttgccagagc atatttctgc
     2581 cgctcttttg aaaccgccgt gctgctaaca tatttacctt cgttagtttc aattcggctg
     2641 ctccaaatcc tctgttcctt agatgtctgg ataatgtgct ccgggttatt tcgcctttac
     2701 ctacaatacc ttcgccttca aggatatgta taatcctctc tacgcttctt cccggaagtt
     2761 cctgctttaa cgatattgca taatccaaaa cttcctccgt tatcgcctta agctgccctg
     2821 cgtcgtctct ttctttcggc ttcaatccct catagccctc ttcacgatat gctgccaggt
     2881 atcggcggag tgtcctttcg gatatgtcct cccgttccaa tatctcatgc cggattcttc
     2941 tcttttctgc tgcatccagc tccggtatca atagaggtgc aatcatctca tgccttttta
     3001 aggcgnnnnn nnnnnaaggc ggtttctatg atttcattac ttgccatttt catccctccg
     3061 atagtttttc cttcatccta tcagagagac attccggcca tccacttaat ctatgtggga
     3121 attgctttac aacattatgc ctgtccaata agaagacagc cacatatttg cttcccccat
     3181 aaaaaggccc ccggagtcta tgggtggaaa tgcttccagc acacgcccca atagagcaga
     3241 acccttaact cctgtcatca caagttcatt tactgtttta ttaaatattc tgtaaagcag
     3301 agaacgcagt gctcctactg cctgcggcag tttatctttg tactctgccc accaccgtct
     3361 tactgtttgt acgcttgctt cac
//
LOCUS       DAOSOW010000201         3370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_28276_length_3370_cov_3.529110, whole genome shotgun sequence.
ACCESSION   DAOSOW010000201 DAOSOW010000000
VERSION     DAOSOW010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28276_length_3370_cov_3.529110"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="PLD67_05960"
     CDS             complement(<1..579)
                     /locus_tag="PLD67_05960"
                     /inference="COORDINATES: protein motif:HMM:NF025854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HOT22099.1"
                     /translation="MEEKHKNEMELQQINLERVYLTDVNSALEEIRILRHDMRGELAL
                     IHGYNELNQKDKIRLHIEKKLHEMDVQLIPQIDSENIITSFLNFKIKEAESKNINVEL
                     KSNINADVEIYIDKGDICRVINNIMNNAIEALQDCDTKKMKLFIGTAGGYLIIKSENP
                     YKGSLLTEGKKIITIKKDKTKHGYGLKSINSIA"
     gene            complement(642..1136)
                     /locus_tag="PLD67_05965"
     CDS             complement(642..1136)
                     /locus_tag="PLD67_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22100.1"
                     /translation="MDSWILIEAFVNFLQIYIIYKATGLFYDRRLSFKFSVEIVMVIM
                     TVLLTGLNYYSQIHSNPLLYIAFYLLIFITAAIIYKGNIISKAVIIFLLIVVTGILEL
                     LAAVLITTLGGFDLNTIQEQSNVRLGVMIISQTLYVYFYMFMRKRFGKDKISFFNNKY
                     NILG"
     gene            complement(1152..1859)
                     /locus_tag="PLD67_05970"
     CDS             complement(1152..1859)
                     /locus_tag="PLD67_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT22101.1"
                     /translation="MVNIAVCDDSKEYVEIVRELINVIMVKNNTACNIYTYISGLSLV
                     QDFRVKTFDIIFLDMEMPEIDGIKTGLLIREISDKVIIFYLTSHKEYAYESYQVKAKN
                     YLLKPIQSDVLEKELMECIAELDMPLEFLDVKDTDGIFHRIPLCDITHILRKKEDRKI
                     HIYTLDKQEIIAVQTLKNIENSLSSISFIKRSCKSCLVNLNNIRAIHKNIIVFINGET
                     EEASRRCLSDLVNLFKK"
     gene            complement(2128..2469)
                     /gene="rplQ"
                     /locus_tag="PLD67_05975"
     CDS             complement(2128..2469)
                     /gene="rplQ"
                     /locus_tag="PLD67_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOT22102.1"
                     /translation="MGSHRKLGVKSDHRVAMLRNMTADLITYGRITTTVTRAKELRRV
                     AERIITLGKRGDLHARRQALAYLYDKNAVYKLFEEVAPKYKDRNGGYTRILKLGPRRG
                     DGAEMAIIELV"
     gene            complement(2488..>3370)
                     /locus_tag="PLD67_05980"
     CDS             complement(2488..>3370)
                     /locus_tag="PLD67_05980"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOT22103.1"
                     /translation="IVVEPLERGYGTTIGNSLRRILLSSLPGAAVTSINIQGVLHEFS
                     TIPGVREDVTEIILNIKNLAAKMNVPGPVQLLIDAKGPKEVTAADIITGGDVDIINED
                     LHIATLEEGSELIIEMEMEQGRGYAIAERNKKDSQAIGVIPIDSIYTPVKKVNFTVED
                     TRVGNRTDFDKLTLEVWTNGTIEPEEAVSLGAKILNEHLNLFISLTEHVNEVEIMVEK
                     EEDEKEKVLEMTIEELDLSVRSYNCLKRAGINTVEELTYKTEEDMMKVRNLGKKSLDE
                     VSKKLEELGLSLRKNDN"
BASE COUNT          956 a          676 c          462 g         1276 t
ORIGIN      
        1 agctatactg ttaatacttt ttaagccgta gccatgttta gttttatcct ttttaattgt
       61 aataattttt ttgccttcgg ttaataagct gcccttataa ggattttctg attttataat
      121 taaatagcct cccgctgtcc cgataaagag cttcattttt tttgtatcac aatcttgtaa
      181 tgcttcaatt gcattgttca ttatattgtt tataaccctg caaatatctc ccttgtcgat
      241 gtatatttct acatcagcat tgatattgct ttttaactca acatttatat tcttggactc
      301 cgcttccttt attttgaaat ttaagaatga agttataata ttctcgctgt ctatttgagg
      361 aataagctgt acatccattt catgaagttt cttttctatg tgcaatctga ttttatcctt
      421 ttggtttaat tcattgtaac catgtattag tgctaactct ccgcgcatat catgtctcag
      481 aattcttatt tcctccaagg cggaattcac atccgttaaa tacactcttt ccaaattgat
      541 ttgctgaagc tccatttcgt ttttatgttt ttcttccatg tcttttagaa cttggtttat
      601 taaagttata gaagcaatag accataatga aatgcataat gtcagcctag tatattatat
      661 ttgttgttaa aaaagcttat tttatcctta ccaaaccttt tcctcataaa catataaaaa
      721 tatacataaa gagtttggga tattatcatc actccaagtc tgacattgct ttgctcttga
      781 atggtgttta aatcaaaacc tcctaatgtg gtaatcaaca ctgctgccag caattcaagg
      841 attcccgtaa ctactataag caaaaatatt attacagcct ttgaaataat attgcctttg
      901 tatattattg ctgctgtaat aaatatcagt aaatagaaag caatatacaa taaaggattt
      961 gaatgtattt gggaataata attcaatccg gtgagcaata cggtcattat aaccattaca
     1021 atttcaacgc tgaatttaaa cgataacctt ctgtcgtaaa acagtcccgt ggccttataa
     1081 ataatataaa tttgaagaaa attaacaaaa gcctcaatta aaatccatga atccactcta
     1141 tcacctcttg tttatttttt aaataaattc actaaatccg aaaggcatcg tctgctggct
     1201 tcttctgttt caccgtttat aaaaacaata atatttttat gtattgctcg tatattatta
     1261 agatttacaa gacaggattt acaggatctt ttaataaaac tgattgaaga cagactgttt
     1321 tctatgtttt taagagtttg aaccgctata atttcttgtt tatccaaagt ataaatatgt
     1381 atctttctat cttctttttt tctaagtata tgagttatat cgcataaagg aatgcggtga
     1441 aatattccgt ctgtgtcttt tacatcaaga aattcaagag gcatatccaa ttctgcaatg
     1501 cactccatca gttctttctc caatacatcg ctttgaatag gcttaagtaa ataattttta
     1561 gcctttacct gatatgattc atatgcatat tctttatgag atgtcaggta aaaaataata
     1621 accttgtcgc ttatttccct tattaaaagg cctgttttta taccatcaat ttcgggcatt
     1681 tccatatcca gaaaaattat gtcaaacgtt ttcactctaa aatcttgtac aagggataag
     1741 cctgaaatgt atgtatatat attacaggct gtattatttt tcaccattat tacatttatt
     1801 aattccctga ctatttcaac atattcttta gaatcatcac aaacagcaat attaaccata
     1861 tttttccatt gttactccgt gtaattgaaa tgtaattgct agttaataat atcataataa
     1921 tacgattatt tcaatgttta tttatacatt cgtcaaaaat ccataaaatt taagaacggc
     1981 tcattttgcc cgtaagaaca aaagaaccgc tcctctacaa ttgtcattct gagaggtctg
     2041 cccgaaagat tctcatgaga tccttcgctt acgctcagga tgacattgtc tttagatcct
     2101 tcgattttac tcaggatgac aactatatta tactaattcg attatagcca tttctgctcc
     2161 atcgcctctt ctgggcccca attttagaat tctggtgtat ccaccgtttc tatctttgta
     2221 tttaggagca acttcttcga atagcttata tactgcattt ttatcgtata aataagctaa
     2281 tgcctgacgt cttgcatgca ggtctcctct ttttccaaga gtgataattc tttctgcgac
     2341 tcttcttaac tctttggctc ttgtaaccgt agttgttatt ctgccgtaag ttataaggtc
     2401 agcagtcata tttcgaagca ttgccactct gtggtcagat ttaaccccaa gcttcctgtg
     2461 acttcccatt tagtcctcct ttttaagcta attatcgttt ttccttaaag ataatcctaa
     2521 ttcctcaagt ttcttcgata cctcatccaa agattttttc ccgaggtttc ttaccttcat
     2581 catgtcttcc tcagtcttgt aagtaagctc ctcaacagtg tttattcctg ctctcttcag
     2641 acaattgtat gaccttaccg ataaatcaag ttcctctatt gtcatttcca aaactttttc
     2701 tttttcatct tcttcttttt caaccataat ttcaacttca ttaacatgtt ctgtcaatga
     2761 aatgaatagg ttgagatgct cattcaatat ttttgcacct aatgaaactg cttcttcagg
     2821 ctctatcgta ccattagtcc agacctcaag agtaagtttg tcgaaatccg ttctgtttcc
     2881 cactcttgtg tcttcaaccg taaagtttac tttttttaca ggcgtataaa ttgaatcaat
     2941 aggaataact ccgattgcct ggctatcctt cttgtttctt tctgctatag catatcctct
     3001 tccctgctcc atttccattt caataatgag ctcggagcct tcttcaagtg ttgctatatg
     3061 caggtcttca ttaataatgt ccacatctcc gcctgtaatt atgtctgccg ccgtaacctc
     3121 tttcggccct tttgcgtcta tcaatagctg cacaggtcca ggtacattca ttttagctgc
     3181 taaattctta atattaagaa ttatttctgt cacatcttct ctcactccgg gtattgtaga
     3241 aaattcatgc aacactcctt gaatgtttat tgaagtaact gctgctcccg gtaaagatga
     3301 aagaagtatt ctccttaatg aatttccgat tgttgtcccg tagcctcttt ccaggggctc
     3361 caccactatt
//
LOCUS       DAOSOW010000202         3361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_28373_length_3361_cov_3.876891, whole genome shotgun sequence.
ACCESSION   DAOSOW010000202 DAOSOW010000000
VERSION     DAOSOW010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28373_length_3361_cov_3.876891"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..671
                     /gene="hpt"
                     /locus_tag="PLD67_05985"
     CDS             108..671
                     /gene="hpt"
                     /locus_tag="PLD67_05985"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006285938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOT22104.1"
                     /translation="MNNDIKKILIGEKELQSKVAELGAKITEDYKGKDLLIVCVLKGA
                     VVFASDLMREIKLPLDIDFMAISSYGSNTKSSGVVRILKDLNTGIEGRHVLIVEDIID
                     SGLTLAYLAENLRSRRPASVEICTILDKKERRTANLDIKYTGFVVPDEFVVGYGLDYA
                     EKYRNLPYIAVLKEEVYNKTNQSESEL"
     gene            851..2650
                     /gene="ftsH"
                     /locus_tag="PLD67_05990"
     CDS             851..2650
                     /gene="ftsH"
                     /locus_tag="PLD67_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOT22105.1"
                     /translation="MYILIFVVIIMLVQSMVEPPTQVTSISYSDLVVEIQNDNVKTLT
                     FTENEVEGIFKDGKEFTSYIPSLFYFSGAFYDNHLVEKIEAGEIEVIGEPVPPTPLWL
                     QALPTIGMLVLLGVLWYVFMKQSQGGGTGKAMSFGKSRARMVKDDEKKITFADVAGLD
                     EEKEELSEIVDFLKNPAKFVKLGARIPKGVLLVGPPGTGKTYLSKSVSGEAGVPFFSI
                     SGSDFVEMFVGVGASRVRDLFDQAKKNAPCIVFIDEIDAVGRRRGAGLGGGNDEREQT
                     LNQLLVEMDGFSGNEGIIIIAATNRPDILDPALMRPGRFDRQVHVGLPDVKAREEILK
                     IHVRKKPLGDDVNLEIVAKRTPGFTPADLENAMNEAALLTARQNKTVITMDIIEEAIT
                     KVIAGPEKRSKVISDNEKKLTAYHEAGHAVVAKLSSSKDPVHMITIIPRGGAGGFTMY
                     LPEEDKSYRSKSDMEEYIVRLLGGRVAEKLALDDISTGASNDIERATKIARAMVTTYG
                     MSDELGPMSYGTSDDEVFLGRDFNKIRNYSEDVAAKIDREMRRIIDNAYNKTERLLSE
                     NIEKLHRVAQALLEKETITGKEFEILFDGGSIS"
     gene            2857..>3361
                     /locus_tag="PLD67_05995"
     CDS             2857..>3361
                     /locus_tag="PLD67_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOT22106.1"
                     /translation="MYDKEKLSHIKEARQQWEENVLAKTLKKAPESKEEFKTVSGSAV
                     NRLYTPEDIADIDYENEIGYPGQYPFTRGYQPTMYRGKPWTMRMYAGFATAEESNERY
                     KYLVSQGSTGLSVAFDLPTQIGYDSDHPLAQGEVGKVGVAIDSLKDMEILFNGIPLDK
                     VSTSMTIN"
BASE COUNT         1210 a          505 c          769 g          877 t
ORIGIN      
        1 tgcagatgat gaaaatataa tatggcttga gggctataga attaatgata aatacaaggt
       61 atcagctgca actaaaaaaa ttctgaatat ttcactaggg aggcaaaatg aataatgata
      121 ttaagaaaat tctgattgga gaaaaagaat tacagtcaaa ggtagctgaa cttggagcaa
      181 aaattacaga ggattacaag ggtaaggatt tattgattgt ttgtgtgttg aaaggtgcgg
      241 tagtttttgc aagtgattta atgagagaaa taaaattgcc tttagatata gattttatgg
      301 ctatttcaag ctatggctcg aatacaaaat cgtccggagt agttagaata ttaaaggatt
      361 taaatacggg tattgaaggc agacatgtgt tgattgtaga ggatattata gattcggggc
      421 tcacattggc ataccttgct gaaaatttaa ggtcgaggcg ccctgcatct gttgagatat
      481 gtacaatact tgataaaaaa gaaagaagaa ccgcaaattt ggacatcaaa tataccggat
      541 ttgtcgttcc cgatgagttt gttgttggtt acggcttgga ttatgcggaa aaatacagga
      601 atcttccata tattgccgtg ttaaaggaag aagtttacaa taagacaaac caatctgaga
      661 gcgaattata agaggaattg tagtacgggg acattcctct atctacagag gctgtcccta
      721 agatagaggt gtgtccccat accgtgaaaa taaataattg tattgattaa atttatatgt
      781 taatattata gtatagtagt cctagactaa gaggaggctt taattgaaag gatttgttag
      841 aagcatcgct atgtacattc tgatatttgt ggtaatcata atgcttgttc aaagtatggt
      901 tgaacccccg acacaggtaa cgagcataag ttatagcgat cttgtagttg aaatacaaaa
      961 tgacaacgta aaaacactta cttttaccga aaatgaggta gaaggaatat ttaaagacgg
     1021 taaggagttt acttcctata taccttcatt gttttatttt tcaggagcat tttatgacaa
     1081 ccatctggtt gaaaagatag aagcaggaga aattgaggta atcggagaac cggtacctcc
     1141 tactccattg tggctccagg cattgcctac aatcggaatg ctggtattgt tgggagtttt
     1201 gtggtatgta ttcatgaaac aatctcaagg gggcggaaca ggcaaggcaa tgagctttgg
     1261 aaagagcagg gctcgcatgg tgaaagatga cgaaaaaaag attacatttg cagacgttgc
     1321 aggacttgac gaggaaaagg aagaacttag cgaaatagtt gatttcctta aaaatccggc
     1381 taaatttgta aaacttggag cacgtatacc taagggagta ctgcttgtcg gacctcccgg
     1441 aacaggaaaa acatatttat caaaatcagt ttcaggtgaa gcaggtgtgc cgtttttcag
     1501 cataagcggt tcggacttcg ttgaaatgtt tgtaggagta ggagcatcca gggtaagaga
     1561 tttatttgac caagcaaaga agaatgctcc atgcattgta tttatagatg aaattgatgc
     1621 agtcggaaga agaagaggag caggtcttgg gggcggcaat gatgagagag agcagacatt
     1681 gaaccaacta ttggttgaaa tggacggatt ctcaggcaat gaaggaataa tcatcattgc
     1741 agcaacaaac agacctgaca ttcttgaccc tgcattgatg agaccgggaa gatttgacag
     1801 acaagtccac gtaggacttc ctgatgtaaa agcaagggaa gaaatattga aaatccatgt
     1861 aagaaagaaa cctttgggag atgatgtgaa tttagaaata gttgcaaaaa gaactcccgg
     1921 attcacacct gcagacttgg aaaatgccat gaatgaggct gcacttttga ctgcaagaca
     1981 aaacaagacg gttattacaa tggatattat cgaagaagca attacaaagg taattgcagg
     2041 acccgaaaaa agaagcaaag ttatcagcga taacgaaaag aagctcactg cttatcatga
     2101 agcaggccat gcagttgtag caaagctttc atcatcaaag gacccggttc acatgattac
     2161 gataattccg agaggcggag caggcggatt tacaatgtat cttcctgaag aagacaaatc
     2221 ataccgctcg aaatcagata tggaagagta tatagtgaga ttgctgggag gaagggttgc
     2281 agaaaaatta gctcttgacg atatatcaac aggagcttca aacgatatag aaagagcaac
     2341 taaaattgca agggcaatgg ttacaacata cggcatgagc gatgagctag gacctatgtc
     2401 ttatggaacc agcgatgacg aggtgttctt gggaagagat ttcaacaaga taagaaatta
     2461 cagcgaagat gttgcggcaa aaattgatag agaaatgcgt agaattatag ataatgccta
     2521 taataaaaca gaaagactat taagcgaaaa tatagaaaag ctgcacagag ttgctcaggc
     2581 tcttcttgaa aaagaaacaa taacaggcaa agagtttgaa atactttttg acggcggaag
     2641 cataagttag aaaaaatcga ataagaatac aaggtttgta aataatacag taaggaacgg
     2701 catactgccg ttccatttta ttagtaaaat acctcgattc acttattttt gcaagaaatt
     2761 tttaattaaa atcatttatt cgaaagtgtt gcaaaaaaag tgaattcata ttaaaatata
     2821 gcttgagatt atgcatttag ataaaaggag gcaataatgt acgataaaga aaagctctcc
     2881 catataaaag aagcaagaca acaatgggag gaaaatgttt tggcgaaaac cttaaaaaag
     2941 gcgcccgaaa gcaaggaaga gtttaaaact gtttccggct ctgcagttaa cagattatat
     3001 acacctgaag atattgcaga tattgattac gaaaacgaaa taggataccc tggtcaatat
     3061 ccttttacaa gaggatatca acctaccatg tatagaggaa aaccatggac tatgcgtatg
     3121 tacgcaggat ttgcaactgc agaagaatca aatgaaagat acaaatacct ggttagccaa
     3181 ggctctacag gtctatctgt tgcatttgac cttccaacgc aaataggtta tgactctgac
     3241 catccgttgg cacagggaga agtaggaaaa gtcggagttg ccattgactc attaaaggat
     3301 atggaaatat tgtttaacgg aatccctctt gataaagtaa gtacttcaat gacaataaat
     3361 g
//
LOCUS       DAOSOW010000203         3357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_28423_length_3357_cov_3.885221, whole genome shotgun sequence.
ACCESSION   DAOSOW010000203 DAOSOW010000000
VERSION     DAOSOW010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28423_length_3357_cov_3.885221"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="PLD67_06000"
     CDS             <1..734
                     /locus_tag="PLD67_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010294000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HOT22107.1"
                     /translation="EDIVKPLKKVLRLATEDDIKRNRENKEKEKEAYDICFKKIEEHG
                     LEMKLIDIEYTFDNNKVIFYFTADGRIDFRELVKDLAAIFRTRIELRQIGVRDEAKMI
                     GGLGPCGRHMCCSSFLGEFYPVSIKMAKEQKLSLNPAKISGICSRLMCCLNYEHQVYE
                     ENIKKLPDIGDKVQVLGTNKTGIVIDINPLFVTAKVNVEKSDGTIEVEEIQGSRLKVV
                     EEGVLKHYDAEDTDIDIEELKELED"
     gene            846..1016
                     /locus_tag="PLD67_06005"
     CDS             846..1016
                     /locus_tag="PLD67_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT22108.1"
                     /translation="MAYIITDACISCGACEPECPVGAISAGDDKYVIDAATCIECGAC
                     ASVCPVEAPQQD"
     gene            complement(1091..2785)
                     /locus_tag="PLD67_06010"
     CDS             complement(1091..2785)
                     /locus_tag="PLD67_06010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017894505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOT22109.1"
                     /translation="MKFNEYEYIRPDYNIIKEKYLNLINDFKNSATADEQYTILKKIN
                     AITNNVDTMSQLVYIRNSINTADEFYDTEKDYMDMTSPKFYGLRIEFYKQLLNSKFKE
                     ELTDKIHPQLITLAEMEIKTFSDEAIPLMQEENKLISEYDKLISSAKIEYDGKLLNLS
                     QMQPYMESKDRRIRKEAHEKSSNFFKENETKFDLIYDKLVKIRHQIALKLGFNNFIEL
                     AYLRNGRSDYNSTDVRNFREHVLKYIVPITNELRERQAKRIGVDEFKYYDIPLNFISG
                     NPTPKGNKDWIMERTKKMYEELSPETHEFINIMIDRELFDLETKPNKQAGGYCTFLHD
                     YKSPFIFANFNGTQDDVTVVTHEAGHAFQTYQSRHFDAPEYSFPTSEAAEIHSMSMEF
                     ITWPWMELFFEKDIEKFKFFHLADSLCFIPYGVTVDEFQHIVYEYPDMTPDERKKAWR
                     DIEKKYTPYKNYDDNEFLNKGTFWFKQGHIFSSPFYYIDYTLAQICSYQFWLRFNENR
                     KEAWEDYLKICKIGGSQSFLEILKAGNLESPFEESTINSVASKIKAYLNTIDDMKL"
     gene            2999..>3357
                     /locus_tag="PLD67_06015"
     CDS             2999..>3357
                     /locus_tag="PLD67_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011722324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOT22110.1"
                     /translation="MKNIIVKVLKNSIAEELNIEPGDELLSVNNSIIKDYIDYKYQIS
                     DNLVYFEIKKKDGEIWELEIEKDYDEDIGIVFENPLMDNIKVCSNKCIFCFIDQMPKG
                     MRKTLYVKDDDTRLSFL"
BASE COUNT         1121 a          496 c          605 g         1135 t
ORIGIN      
        1 aagaagatat tgtaaagcca ttaaaaaaag tactaaggct tgcaacagag gatgatatta
       61 aaagaaaccg agaaaataaa gaaaaagaaa aagaagctta tgatatttgc ttcaaaaaga
      121 tagaagaaca tggtttggaa atgaagctca ttgatataga atatactttc gataataaca
      181 aagtaatttt ttatttcacg gctgacggca gaatagattt cagggaatta gtaaaagatt
      241 tagcggcaat attcagaaca agaatagaat taaggcaaat cggtgtcagg gatgaagcta
      301 aaatgatagg aggtcttgga ccttgcggca gacatatgtg ctgctcatcg tttttgggtg
      361 aattttatcc ggtatccatt aaaatggcaa aagagcaaaa attatcttta aatcctgcca
      421 aaatatcagg aatatgctcc aggctcatgt gctgtttaaa ttacgagcat caggtatacg
      481 aagaaaatat taaaaagctt ccggatatag gagataaagt acaggtgttg ggaacaaata
      541 aaacaggtat agtaatagac attaatcctt tatttgtaac tgcaaaagtt aatgtagaaa
      601 aaagtgacgg aactattgaa gtagaagaaa ttcaaggttc acgactgaag gttgtggaag
      661 aaggtgtcct gaaacattac gacgcggaag atacggacat agatatcgaa gaattaaagg
      721 aattggaaga ttaataattt cacaccttat ataaggtgtg aaattattat gaaaaaacac
      781 tttaaaatta ttttaaatag tgttacaata agtggtgatg accatattgg gggaggtgaa
      841 tttaaatggc ttacataata acagatgcat gtataagctg tggagcatgt gaacctgagt
      901 gccctgtagg tgcaatttca gccggtgatg acaaatatgt gatagacgct gcaacttgta
      961 ttgaatgcgg agcatgcgcc agcgtatgcc cagtggaggc tcctcaacag gactaataaa
     1021 ttaataaaag ggactgatta aagtgtgtat gtatacgctt taatcagtcc cttttatatt
     1081 tccagaatcc ttacagtttc atgtcatcaa tcgtatttag gtatgcttta atttttgatg
     1141 caactgaatt aatggttgat tcttcaaagg ggctttctaa gttaccggct tttaaaatct
     1201 caaggaagga ttgactcccg ccgattttac agatttttaa gtaatcttcc catgcttcct
     1261 ttctgttctc attaaacctt aaccaaaatt gataggagca aatttgtgcc agagtataat
     1321 cgatgtaata aaagggagat gagaaaatat gaccttgttt aaaccaaaaa gtacctttat
     1381 ttaaaaattc attatcatca taatttttat aaggggtata ttttttttcg atatctctcc
     1441 atgctttttt tctttcatca ggagtcatat caggatactc ataaacaata tgctgaaatt
     1501 cgtcaacggt cactccataa ggtatgaagc acaaagaatc cgccaagtgg aaaaatttaa
     1561 atttttctat gtctttttca aaaaataatt ccatccaagg ccatgtaata aattccatgc
     1621 tcattgaatg tatttcggca gcttcagacg tagggaaact gtattcgggc gcatcaaaat
     1681 gtctgctttg gtatgtttga aaagcatggc ccgcttcatg agtaacaacg gttacatcgt
     1741 cttgagtgcc gttaaaattg gcaaatatga aaggactttt gtaatcgtga aggaatgtgc
     1801 aatatcctcc tgcctgctta tttggctttg tttccaagtc aaataattct ctgtcaatca
     1861 ttatatttat aaattcatga gtttcgggag acaattcctc atacattttc ttggtgcgct
     1921 ccattatcca gtctttattt ccttttggag ttggatttcc tgatatgaaa ttcaggggaa
     1981 tgtcatagta tttaaattca tcgactccta tgcgctttgc ctgtctttct cttaattcat
     2041 tcgtaatcgg tactatgtat ttcaatacat gttctctgaa atttcgaaca tctgttgaat
     2101 tgtaatcaga ccttccgttt cttaaataag caagctctat aaaattatta aatcctaatt
     2161 ttaatgctat ttggtgtcta atctttacca atttatcgta tatcaagtca aatttagttt
     2221 cattttcttt gaagaaattg ctgctttttt catgagcttc ttttctgata cggcggtcct
     2281 ttgattccat atagggctgc atttgagaca agtttaataa ctttccgtca tactctatct
     2341 ttgcggatga tatcagttta tcatattcgc ttattagctt attttcttcc tgcattagag
     2401 gaatcgcttc atcagaaaat gttttaatct ccatttccgc taaggtaata agctgaggat
     2461 gtattttatc tgttaattct tccttaaatt ttgaatttaa cagttgtttg taaaattcaa
     2521 tcctcagtcc gtaaaatttc ggtgatgtca tatccatata gtccttttca gtatcataaa
     2581 attcatcagc tgtattgatg gaatttcgta tatacaccag ttgactcatt gtgtcaacat
     2641 tatttgttat agcattgatt ttttttagga tggtatattg ttcatcggca gttgctgagt
     2701 ttttaaaatc attaatcaga tttaaatact tttcttttat gatattataa tcaggtctta
     2761 tatattcata ctcattgaat ttcataatgc gcctcgcttt atatttagtt agctcttaac
     2821 tttacatatt tcatatggca gaaaagataa taatattata acaaaaattg ttaaatatgc
     2881 aagacattag tgtttaaaaa taagattgca gttgtttgtt aagatatggt ataatgataa
     2941 aaccaaatcc ttttggcaca gactgaagaa cttatgttat caattgaaag ggaaattcgt
     3001 gaaaaacatt attgttaaag tattaaaaaa cagcatagcg gaagaattaa atatagagcc
     3061 gggagatgag cttttaagcg ttaataattc cataataaag gattacatag attataaata
     3121 tcaaatttct gataatttgg tttattttga aatcaaaaaa aaagacggag aaatttggga
     3181 actcgaaatt gaaaaggatt acgatgaaga tataggaata gtatttgaaa atccccttat
     3241 ggataatatt aaggtatgct ctaataagtg tattttttgt tttatcgacc aaatgccgaa
     3301 aggcatgaga aaaacacttt atgtgaaaga cgatgatacc aggctttctt ttctgta
//
LOCUS       DAOSOW010000204         3356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_28429_length_3356_cov_3.777946, whole genome shotgun sequence.
ACCESSION   DAOSOW010000204 DAOSOW010000000
VERSION     DAOSOW010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28429_length_3356_cov_3.777946"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..669
                     /gene="mraZ"
                     /locus_tag="PLD67_06020"
     CDS             232..669
                     /gene="mraZ"
                     /locus_tag="PLD67_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOT22111.1"
                     /translation="MFTGEYIHSFDEKGRVIIPSKFRNELGETFYIGKGLDKCLFVYP
                     IETWTEFVGKLKNLSTFNKEERFFLRRFVSGFSECSFDKQGRILIPPQLRSFSELNTD
                     AAIIGVIDRIEIWNKDTWDEYSNNEQFDFDSIAEKMSELGIGL"
     gene            679..1608
                     /gene="rsmH"
                     /locus_tag="PLD67_06025"
     CDS             679..1608
                     /gene="rsmH"
                     /locus_tag="PLD67_06025"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOT22112.1"
                     /translation="MDYIHKPVLLDESVKGLNIKEDGIYVDCTLGGGGHTEEILKKAT
                     KGKVIGIDQDDYAIERASEKLSGYKNFMPVKNNFSKIEEILNELSIDKIDGIIFDLGV
                     SSFQLDIPERGFSYNYDMPLDMRMDKSQTTTARHIVNGYEENELTRILWDYGEEKWAA
                     RIAKFIVEEREQEFIETTGQLVSIIKKAIPKSARQEGSHPAKKTFQAIRIEVNRELEI
                     LENTVKAAVERLTKKGRICVITFHSLEDRIIKNTFAELNKDCICPPEFPKCMCEHRRK
                     LKIITKKPITPSEEELTENRRAHSAKLRIGERV"
     gene            1612..2076
                     /locus_tag="PLD67_06030"
     CDS             1612..2076
                     /locus_tag="PLD67_06030"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOT22113.1"
                     /translation="MSALRKEAVELHEHELEKEYSPKKRIKRNKRNNTFIESLKNSVM
                     IAITFILGILIVYNYAVITDKQMELNNLNIEIENLNNEIDKYNIALESIKNTNSIEEM
                     AKVYLGMNYPTRKQTVFVDFTYGPDAIEEKNIMAKDENLLVGLIDKVISFIR"
     gene            2090..>3356
                     /locus_tag="PLD67_06035"
     CDS             2090..>3356
                     /locus_tag="PLD67_06035"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT22114.1"
                     /translation="MKKRAKSTTNLQNKRRMLFFLVCFFLVTLALVVRLGYIQLIKGE
                     EYRKQAMENWSRDVTISAKRGTIYDAKGKKLAVSINSNTVTCFPADVKKSVNPTVDNS
                     IDEDDSILTAILKKLNRAMFTEETQAEQIPVNTKTPEEIAAILAEILEMDYDEVLEKI
                     TSNYNYVVVKKWITDEQAQKIREQELSGISIIEDNKRVYPYGNFAPYVLGFTNIDQDG
                     LYGVEATYNSYLTGIPGRTVVNTDAYGRELPFGYNEYYESQDGLGVVLTIDEVIQHYA
                     ESAAEKVMADYNAKRATIIVMDPNTSDILAMASKPDYDPNDPRTPIDENIKAEWANLS
                     NEELQNAWFDMWRNPAVNDVYEPGSTFKLITTATALEENVATLGSTYFCDGYVRQITS
                     HNIKCWRYYRPHGQQTLSEAIQHSCNDALA"
BASE COUNT         1277 a          505 c          686 g          888 t
ORIGIN      
        1 gagcgaagcg aaggatctca tgagattctt cgagctaaag ctctcagaat gacacggatg
       61 agattcttca gctaaagctc tcagaattac aggcaagtac ttttcgctgc gttaaaatga
      121 caagtatttc actcaaaaaa atgacaaaaa aaaatttttt gtatttttta aaaataattg
      181 tggcatttta tacccactat gtggtataat acacccagta ggtggtagaa aatgtttact
      241 ggtgaatata tacattcatt tgatgaaaaa ggaagggtta taattccttc aaaatttaga
      301 aatgaactag gagaaacatt ttatattgga aaaggtttgg ataaatgctt attcgtttat
      361 cctattgaaa catggacaga attcgttggt aagcttaaaa acttatctac cttcaacaag
      421 gaagaaagat ttttcttaag acgatttgtt tcaggttttt ctgaatgttc tttcgataaa
      481 cagggaagaa tattaatacc gcctcaactt agaagtttca gcgaattaaa tacagatgct
      541 gcaattatag gggttataga cagaattgaa atttggaata aagatacttg ggatgaatat
      601 tccaataatg agcaattcga ttttgacagc attgccgaaa aaatgtcgga attggggata
      661 ggcttataag gaggaagcat ggattacata cataaaccgg ttttacttga cgaatccgta
      721 aagggactga atataaaaga agacggtatt tatgttgatt gcactcttgg ggggggaggc
      781 catacagaag aaatcttaaa aaaggcaact aaggggaaag tcataggaat tgaccaggat
      841 gattatgcta ttgaaagagc gtcagagaaa ttaagcggct acaaaaattt tatgccggtg
      901 aaaaataatt tcagcaaaat cgaagaaatt ttaaatgaac ttagtattga caaaattgac
      961 gggataatat ttgaccttgg tgtttcgtca tttcaactgg atataccgga aaggggtttt
     1021 tcttataatt atgatatgcc ccttgacatg aggatggaca aaagtcagac aaccactgcg
     1081 agacatattg taaacggtta tgaagaaaat gaattgacaa gaattctttg ggattacggt
     1141 gaagaaaaat gggcagcaag aattgcaaaa ttcattgtag aggagagaga gcaggaattt
     1201 attgaaacaa cggggcagct tgtatcaatt attaaaaaag caatacctaa atcagccagg
     1261 caagaaggct cccatcctgc taagaaaaca tttcaagcaa taagaattga agttaaccga
     1321 gagttggaaa tacttgaaaa tacagttaag gctgcagttg aaagattgac caaaaaaggg
     1381 agaatctgcg taataacatt tcattcactt gaggatagaa ttataaaaaa tacatttgcg
     1441 gaattaaaca aggattgtat ttgtcctccc gagtttccca aatgcatgtg cgaacacaga
     1501 agaaaactaa aaataattac caaaaaacca ataactccgt cagaagaaga attaacagaa
     1561 aacagaagag cacacagcgc gaaattaaga ataggtgaga gggtgtaaag aatgtcagca
     1621 ctgagaaaag aagctgttga gttacatgag catgaattag aaaaagaata ttctccaaaa
     1681 aagcgaataa aaagaaataa gagaaataat acttttatag agagtttaaa aaattctgtc
     1741 atgatagcaa ttacttttat attaggaatt ttaatagtct ataattatgc cgtaatcact
     1801 gacaagcaaa tggagctgaa taatctcaat attgaaatag aaaatcttaa caacgaaatt
     1861 gacaaatata atatagcttt ggagagtatt aaaaacacta acagcattga agaaatggca
     1921 aaagtttatt taggtatgaa ctatcccaca aggaagcaga cagtatttgt agactttaca
     1981 tatggccctg atgcaattga agaaaagaat ataatggcaa aggatgaaaa tctgttagta
     2041 ggattaattg acaaggtaat aagtttcatt cgataagggg gattgccata tgaagaagcg
     2101 tgcaaaaagc actactaacc ttcaaaataa aagaaggatg ttgttttttt tagtatgctt
     2161 ttttttagtt acactagctt tggttgtaag actcggttac atacagctga taaaaggtga
     2221 agaatataga aaacaagcaa tggaaaactg gtcaagggat gttaccataa gcgccaaaag
     2281 gggaactata tacgatgcta aaggtaaaaa attagcagtc agcataaatt ccaacactgt
     2341 cacatgtttt cccgcggacg ttaagaaaag cgtaaatcct accgttgata attcaataga
     2401 tgaagatgac agtattctta ctgcaatttt aaaaaagctt aacagagcta tgtttactga
     2461 ggaaactcaa gctgaacaaa tacctgtaaa taccaaaaca cctgaagaaa tagctgcaat
     2521 tctggcagaa attttagaga tggattacga tgaagttctt gaaaaaataa catccaatta
     2581 taactatgtg gtagtaaaaa aatggattac agatgagcag gcacaaaaaa taagggagca
     2641 ggaattaagc ggcataagta taatcgaaga caataaaagg gtttatccct acggaaactt
     2701 tgcaccctat gtgttaggct ttacgaacat agaccaggac ggcttatatg gagttgaagc
     2761 cacatacaat agttatctga cgggaatacc gggaagaaca gtcgtaaata ccgatgctta
     2821 cggaagagag cttccttttg gctataatga atattatgaa tctcaggacg gattaggtgt
     2881 tgtgcttacc atagacgagg taatacagca ttatgctgaa tctgcagccg agaaggttat
     2941 ggcagattac aatgcaaaac gagcaacaat catagttatg gaccctaata ctagcgatat
     3001 tttggcaatg gcatcaaagc ctgattatga ccccaatgac cccagaacac caatagatga
     3061 aaacattaaa gccgaatggg caaatttatc taatgaagaa cttcaaaatg cctggtttga
     3121 catgtggcga aatcctgctg ttaatgatgt atacgagccc ggttcaacat ttaagctgat
     3181 aaccacggca acagctcttg aagagaatgt tgctacactt ggctcaacct atttctgcga
     3241 cggttatgta agacaaatca caagccataa tattaaatgt tggagatatt acagacctca
     3301 cggacagcaa actttatcgg aggcaataca acattcatgc aacgatgcac ttgcac
//
LOCUS       DAOSOW010000205         3331 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_28711_length_3331_cov_2.765263, whole genome shotgun sequence.
ACCESSION   DAOSOW010000205 DAOSOW010000000
VERSION     DAOSOW010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28711_length_3331_cov_2.765263"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="PLD67_06040"
     CDS             complement(<1..886)
                     /locus_tag="PLD67_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22115.1"
                     /translation="MGPIDLIIFALYMIGMVVIGFIAQGKIETMDDFILGGRRFGLVA
                     LVGTIMATMIGSGMTMGAVGNAYKAGAGGTVFWMYTGFSIGLLYFGYIAGKIRETGKR
                     TVAEVLSAKFGNNTRLISSIVIIGYAVSIVAINIAGLRNVIFYVFGSKLGISLPLATT
                     IAAAICILYTSLGGFYAVVWTDVVQFGLMLLGIVILGPIIGLNSAGGFNNVVLEYEKI
                     GSSITNPFINGISSGSIGFFLAYFLTVPGDPTMPQRALAAKDEKSAKISFYISGVLGF
                     FFGFALLIMGGSAFVLMPG"
     gene            complement(931..1860)
                     /locus_tag="PLD67_06045"
     CDS             complement(931..1860)
                     /locus_tag="PLD67_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOT22116.1"
                     /translation="MNICIIDAKQFITDEKLIEPLYKLGNVSMYDGIPQSLDETIKRA
                     KDADIVLFALMKFTNEIIDNLPNLKILQFIGTGVWNFVDALYAEKKGIKVLNIEGYGN
                     NAVAEFALSCAFSLARKITNADRILKERRWSLDNLEGIEIEGSVFGVVGTGNIGSIVA
                     KKACLLGADVIACDIYESDELKNKYGIKYVSLSEIFEKSDIITIHMKATKETEKIIGK
                     DLLSKMKKGSLFINVARAELVDNESLYDLLKSGQIAGAAIDVYENEPPKDYKLAELEN
                     VIATPHIGFYTKKANDNSIRLSVESILNFLDID"
     gene            complement(1886..3051)
                     /locus_tag="PLD67_06050"
                     /pseudo
     CDS             complement(1886..3051)
                     /locus_tag="PLD67_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160144.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
     gene            complement(3162..>3331)
                     /locus_tag="PLD67_06055"
     CDS             complement(3162..>3331)
                     /locus_tag="PLD67_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase"
                     /protein_id="HOT22117.1"
                     /translation="AYGANCIWVNDTVIVPEGYPTVEKGVRDTGYKVIVVDTSEYRKL
                     DGGLSCLSLRF"
BASE COUNT         1054 a          684 c          410 g         1183 t
ORIGIN      
        1 atcctggcat caacacaaat gcagaaccac ccataattaa taaagcaaat ccgaaaaaga
       61 aacctagcac acctgaaata tagaatgata tttttgcaga cttttcatcc tttgcagcta
      121 aagctctttg aggcattgtc gggtctccgg gtacagtaag aaagtatgcg aggaaaaagc
      181 caattgagcc tgagcttata ccgttaatga atggatttgt aattgatgaa cctatttttt
      241 catattctaa tactacattg ttaaatcccc ctgcgctatt aagacctata ataggaccta
      301 atataacaat gcctaacaac attaatccaa actgaactac atctgtccaa acaactgcat
      361 aaaacccacc taatgatgtg tataaaatac atatagctgc ggctatggtg gtggctaagg
      421 gcaatgaaat cccaagcttg ctgccaaata catagaatat aacatttctt aaaccggcta
      481 tatttattgc aacaattgat accgcatatc ctataattac gatagacgat attaatcttg
      541 tattattgcc aaatttagca gataatactt ctgcaacggt tcttttgcct gtttctctta
      601 ttttgcctgc tatataacca aaataaagca aaccaataga aaaacctgta tacatccaga
      661 aaactgttcc accggcaccg gctttgtacg catttcctac cgcacccatt gtcataccgg
      721 agcctatcat agttgccata atagtaccaa ccaaagcaac taaaccaaat cttcgccctc
      781 caagaataaa atcatccatt gtttcaattt taccttgtgc aataaatcct attacaacca
      841 tccctatcat ataaagtgca aaaataatta aatcaatagg tcccatatta atcctcctct
      901 aataatatct ttttataatt attgattttt ttaatcaata tctaaaaaat tcaaaattga
      961 ttctactgat aatcttattg agttatcgtt tgcttttttt gtgtaaaaac ctatgtgcgg
     1021 agttgcaata acattctcta attctgccag tttataatcc tttggaggct cattttcata
     1081 tacatcgatt gccgcgccgg ctatttgacc actcttcagc aaatcataca gtgattcgtt
     1141 atcaactaat tcagctcttg ctacatttat gaataatgag ccttttttca ttttagacaa
     1201 taaatctttc ccgataatct tctctgtttc tttcgttgct ttcatatgta ttgttataat
     1261 atcagatttt tcaaatattt cagataaact gacatattta ataccatact tgtttttcaa
     1321 ttcatccgat tcataaatat cacaagcaat aacatcagca cctaacagac acgccttttt
     1381 agctactatc gagccgatat ttcccgtacc gactacacca aacactgaac cttcaatttc
     1441 aataccttcc agattatcca aagaccacct tctttctttt aatattctgt ctgcatttgt
     1501 aatttttctg gctaaagaaa atgcgcagct taaagcaaat tctgctacgg cgttattacc
     1561 gtaaccttca atgtttaaaa ccttaatgcc ttttttctca gcatacaacg catcaacaaa
     1621 attccaaact cccgtaccta taaattgcaa aatctttaag tttggtagat tatcaattat
     1681 ttcatttgta aacttcataa gcgcaaataa aacgatatct gcatctttag cccttttgat
     1741 tgtttcatcc aaactttggg gtatgccatc atacattgaa acatttccta atttatacaa
     1801 gggttctata agtttttcat ctgttataaa ctgcttagca tctataatac aaatattcat
     1861 aacttcatcc tttcaaacaa aacaattacc taaaaatatc cattttattt attctgtcaa
     1921 atgcttcgcc taatttatca ataccaactg tacatgctat ccttatatac ccttctccgc
     1981 tacttccaaa accacttcca agtattgtaa gaacatgtgc ttcatcaagt atcttcatag
     2041 aaacctcttg agaagataaa cctgtctttt taatattagg aataaataaa atgtacctct
     2101 tggtggtaag acactcatat tttttgtttg acatatcctg tcataggtat aaaaaactct
     2161 ttccttaaaa atattaaaaa gtttgggttg aataatcttt ctatttctaa tagcatgtaa
     2221 cattgctctt tgagaaacgg atggcgctgt aaatacattg ttttcattta ctttttccat
     2281 tgcatccact aaataatccg gagctattaa atatccgatg cgccatccgg tcattgcata
     2341 atctttcgaa gcactgcata tagtaattgt tctttctttc atatttttga tactcataaa
     2401 tggtgtgttt ctattttcac tgtcaaaggt atataatcca taaatatcat caactattac
     2461 gataagatca tatttttcag ctatgtctgc tatttttaat aatgtttcat cactaaacct
     2521 tgctcctgaa ggattgttgg gtgaatttat tattattgct tttgtacgat ttgtaattaa
     2581 tttttccaat ctttcaacat tgatttgaaa accctcttct tcaaatgttt ccaaaaaaac
     2641 cggtatgcct cttgttagct ttatttgatc gggataagga gtaaagtatg gttcatgaat
     2701 aataatttca tcgccatcat ctgttatggt ctcaagtgca agccataaac cttgggcagc
     2761 acttgttgag ataaatgttt catttcgttc aacttttata ttgaactcat catgataata
     2821 cttacatatt tcatctctta attcatcgtc tccataaaaa cttgtataat gtgtatgacc
     2881 attcagtgca tccttaaatg cttccttgat aataatttca tccgtagtaa agtccgggtc
     2941 tccaaatgtc aaatcaataa catcattata tttgctcatt aaaggaccta tttctcccat
     3001 tgcagatgat ttcgtattcc aatatctttt agctataaat ttatgtttca tataaacctc
     3061 ctttgaataa cattctatta acgctttcct tatccgtctt aatttcctgc aatattatct
     3121 ctcataattt tttagcattt gcgtatataa tgatgtattt gttagaatct taatgataaa
     3181 cagctcaatc caccgtctaa ttttctatat tctgacgtat ctactacaat aactttatat
     3241 cctgtatctc ttacaccttt ttcaacagtt gggtaacctt ccggcactat aactgtatca
     3301 ttaacccaaa tgcaatttgc accataagct t
//
LOCUS       DAOSOW010000206         3328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_28735_length_3328_cov_4.125878, whole genome shotgun sequence.
ACCESSION   DAOSOW010000206 DAOSOW010000000
VERSION     DAOSOW010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28735_length_3328_cov_4.125878"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..767)
                     /locus_tag="PLD67_06060"
     CDS             complement(240..767)
                     /locus_tag="PLD67_06060"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOT22118.1"
                     /translation="MVIIMDDMKVKDMVYTALFAALVCVATFIIKVPLPVSGGYAHIG
                     DGFIFLAVILIGKKNGAWAGAIGAALADIIGGYSIYALPTFIIKGVMALIMGYMIEKL
                     PSLKHNWVAGAITGSIWQVLSYYVVGSLMVGNFLTTIGEIPGNTAQSVVGIIVSAAFL
                     EVFKHTPIGKKILHC"
     gene            complement(939..1202)
                     /gene="ffs"
                     /locus_tag="PLD67_06065"
     ncRNA           complement(939..1202)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLD67_06065"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(1253..3076)
                     /locus_tag="PLD67_06070"
     CDS             complement(1253..3076)
                     /locus_tag="PLD67_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT22119.1"
                     /translation="MNYYEEESYSSFNIKPWLKLGRFFKPYTGKLLGIIFFMFITALM
                     DLSIPLFQKYAVDSFIAKNTTEGINGFIALYTAVLVIMAISVNIFIRLAIVIEMHIGM
                     DLRKEIFEHLQNLSLSYYNTTPVGYIMARTLSDTNKIGSMIAWGLVDVFWSFAYVTGA
                     FIAMLLLNVQLTVIIAIVIPVMAVITVYFQNKILLTSRKLRKINSKMTGSFNEGIIGA
                     TTSKTLVIEDKNLVQFKNISSEMKMTAVNLARLNALYIPIIVFFGSVLTALVLAKGGR
                     LAMDKIIQIGTLSAFLSYAISIFEPVQQLARTFAEVISLQANIERVTDLLEKEPLIKD
                     SEEVVKIYGDTINPKKENWEEIIGEIEFKNVSFKYPDGDEEVLQNFNLHIPAGTNVAI
                     VGETGAGKSTLVNLACRFFEPTEGEILIDGKDYRERSQLWLHSNIGYVLQDPHLFSGT
                     IKENIRYGKLDATDEEIMEAAKIVSVDTIINKLEKGYDSHVGEGGDRLSTGEKQLISI
                     ARAILSNPKIFILDEATSSVDTQTEILIQKGIEYLLKGRTSFIIAHRLSTIKKADIIL
                     VVNEGKIIEQGTHNELIAKRGHYYNLYTKQFEEEKTLEVLK"
     gene            complement(3073..>3328)
                     /locus_tag="PLD67_06075"
     CDS             complement(3073..>3328)
                     /locus_tag="PLD67_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006525869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT22120.1"
                     /translation="FDDSLSAVDADTDAKIRSSLLKSKGSSTIIIISHRITTLMESDT
                     ILVLDKGEVSDIGTHEELINRDGIYKDIFNVQMNLSEDIE"
BASE COUNT         1027 a          697 c          514 g         1090 t
ORIGIN      
        1 tgttgacagc ataaaaatta ctgccgaaaa taaagaaata actgatttta ttgatgtgct
       61 ggatacaaca aaaatattat atcctccgga atttattaat ccggaagcag tagaagagaa
      121 agaagagtaa tcaaaaaaaa gtaagctatg tcattctgaa gcctcagaat gacatgcagc
      181 ttattaattg caataacagg ctttgcctaa tggttattca tacaatcgcc cttgtctttc
      241 taacaatgaa gaatcttttt gccgataggt gtatgtttga aaacttccaa aaatgctgct
      301 gatactatta tgcctacaac agactgagct gtattgccgg gtatttcacc gatggttgtt
      361 aagaagttac ctaccatcaa ggaacctacc acataatatg aaagtacctg ccaaatgctt
      421 cccgttattg caccggcaac ccaattatgc ttaagggatg gaagcttttc aatcatataa
      481 cccataatta aagccataac acctttaatt ataaaagttg gcaatgcata aatactgtac
      541 ccgccgatta tatcagcaag agccgcgcct attgcaccag cccatgcacc atttttcttg
      601 ccgattaata ttaccgcgag aaatataaat ccgtctccta tgtgagcgta ccctcctgat
      661 acaggcagcg gtacttttat tataaaggtt gcaacacaaa ctaaggcagc aaacaaagct
      721 gtgtatacca tatctttaac tttcatgtca tccattatta tcaccatccc ataatttact
      781 tccggttaat ctaattatat atcaatctgt actctttaaa agatacagat tgcaaattta
      841 ttcggatgac agattaatat ttaaaataaa agactcttca tgaccaaaat gactacctgt
      901 cattttgagg gcgcagcccg aagaatctca ttaaaaagct gtgcaccctt cttcgacaat
      961 acataacaag cgttacttca gcagttagct caaacccggc accctcacgt cacacaaaag
     1021 taactactta ctgctgcttc cttccggacc tgacagggtt cataggtttt tgttgcgcga
     1081 gaccgagtta tcaacaccac ttgctaaagg caggcctcac tatgcaagcc tcggagaggg
     1141 aattccacct tgctgcagcg gattgcaagt acagggcacc gctacctccc catgtagcac
     1201 agcacaaaaa tgataacata attaaaatca tgtgtcaatg ttataatttg cctcatttta
     1261 gaacttcaag ggttttttcc tcttcaaact gtttagtata taaattataa taatggcccc
     1321 ttttagctat cagttcattg tgtgtccctt gctctattat tttgccctca ttaaccacta
     1381 atataatatc tgccttttta atagttgaaa gtctgtgagc aattataaat gaagttctgc
     1441 cttttaacaa atattcaatt cctttttgga taagtatttc tgtttgtgta tctaccgaag
     1501 atgttgcttc atccaaaata aaaattttag ggtttgaaag tattgccctt gctatggaaa
     1561 tcaactgctt ctcacctgta gaaagcctgt ctcccccttc gccgacatgg ctgtcgtaac
     1621 ccttttccaa tttgttgatt atggtatcga ctgaaacaat ttttgcggct tccattattt
     1681 cttcatctgt tgcatctaat ttaccgtatc ttatgttttc cttgattgtt ccggaaaaca
     1741 aatgcgggtc ttgcagcaca tatccaatgt tcgaatgaag ccaaagctga gatctttctc
     1801 tgtaatcctt gccatctatc agtatttcac cttcagtagg ctcgaaaaat ctgcatgcaa
     1861 gatttaccag agtgctttta ccggcacccg tttcacctac aattgcaaca ttggtacctg
     1921 cgggaatatg aagattaaaa ttttgcaaaa cctcttcatc tccatccgga tatttaaaac
     1981 tgacattttt aaattcaatt tcccctatga tttcttccca gttttccttt tttggattaa
     2041 tagtgtctcc gtaaattttc actacttctt cactatcttt tattaagggt tccttttcca
     2101 ataaatccgt tactctttca atattagcct gcagtgatat gacttctgca aatgttcttg
     2161 ccaactgctg aacaggttca aatatgctta ttgcataaga aagaaaagca gacagtgttc
     2221 ctatttgaat aattttatcc attgccaatc tgcctccttt tgccaagacc aatgctgtca
     2281 aaacggagcc gaaaaatact attatgggaa tgtacaaggc atttaatcgt gctaaattaa
     2341 cagccgtcat cttcatttca ctgcttatat ttttgaattg caccaagttt ttgtcctcta
     2401 ttaccaatgt cttggaggtt gttgcaccta ttatcccttc attgaaactt cctgtcattt
     2461 tagaattgat ttttcttaat tttctgctgg ttaataaaat cttattttga aaataaacag
     2521 ttataaccgc cattacagga attacaatcg caataataac agtaagttgc acattcagca
     2581 aaagcattgc aataaaggca cctgttacat aagcaaatga ccaaaataca tctaccaatc
     2641 cccatgcaat catacttcct attttattgg tatcgcttaa agttcttgcc attatataac
     2701 ccacgggagt tgtgttgtaa tatgacaacg aaagattttg aaggtgttcg aatatttcct
     2761 ttcttagatc catcccaatg tgcatttcaa ttactattgc caatcgtata aatatattta
     2821 cggatatggc cataatcact aagactgctg tataaagagc aataaaacca tttatacctt
     2881 cagtagtatt ctttgctata aaactgtcca cggcatattt ttgaaacaaa ggaatggata
     2941 aatccattag ggcggtaata aacataaaaa agataatacc caacagtttc cctgtgtagg
     3001 gtttaaaaaa tcttcctaat tttaaccaag gctttatatt aaatgagcta taactctctt
     3061 cctcataata attcattcta tatcctccga taggttcatc tgaacattga atatgtcttt
     3121 ataaatacca tctctgttta ttaattcttc atgggttcct atatcggata cttctccctt
     3181 gtccaagact aaaatagtat cagattccat taaagttgtt atgcgatgtg aaataataat
     3241 aattgtcgaa ctgcccttgc tttttaaaag tgatgaacga atttttgcat ctgtgtcggc
     3301 atcgactgca gacaatgaat catcaaac
//
LOCUS       DAOSOW010000207         3327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_28746_length_3327_cov_3.892115, whole genome shotgun sequence.
ACCESSION   DAOSOW010000207 DAOSOW010000000
VERSION     DAOSOW010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28746_length_3327_cov_3.892115"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PLD67_06080"
     CDS             complement(<1..991)
                     /locus_tag="PLD67_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024201.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HOT22121.1"
                     /translation="MKAIIVEIKGRHAAVLTDDGVVSKIKNRNYCIGQEIVIKNNRNK
                     LIRMTAAAAAAIMLFVTPAWAYLTPYSYVSIDVNPSFEFLINRFDRVLTVRGFNDDGR
                     ELSKDINIDGLKNKEVQNAVKSVLNELKNKGYIIEGQEGGVIIATSSKSEAKKDVLFE
                     KLRTAVNEEEAPEQTKDNEIKTKIEEPKEPESTEKLEEKEESEAQQTETIEKTEELTD
                     DKSEKPGNRERPERRDKSESKEDTERREEPEGREKSERREESERSEKSEKPNKYEKPN
                     EKDRKKNVIEVIEVTKKEVDEAKKLKVTPGKLTLIKKLQEAANEVHRGYEIDQD"
     gene            complement(972..1703)
                     /gene="sigI"
                     /locus_tag="PLD67_06085"
     CDS             complement(972..1703)
                     /gene="sigI"
                     /locus_tag="PLD67_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-I factor"
                     /protein_id="HOT22122.1"
                     /translation="MSDLDLLAVEAKTNTDLMNNLIIQYENFILKCASSTTRSYISKS
                     DDEWSIALSAFTEAIKNYSADKGSFLNFAELVIRRRMIDFIRSKSRYAPEVLVNPSLF
                     NTDSQDEEEYSLQSEIAKKVSIVENNSIKMEIALINEIFSDYGFSFMDLTECSPKSHK
                     TKKSCAKVVAYLIKNPILTREIKIKKQLPLNIIEKYTNVPRKLLERHRKYIIAALEIM
                     SGDFPNLAGYMEYIRQELKNESNNS"
     gene            complement(1854..2093)
                     /locus_tag="PLD67_06090"
     CDS             complement(1854..2093)
                     /locus_tag="PLD67_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22123.1"
                     /translation="MKILNEPIKVMAVFNTNGKIEPVKFRLDDKVVRIEKIIKTYEEN
                     IVGNTRIVFVCLHKGKDIFELKYEVASKTWYLFKK"
     gene            complement(2104..>3327)
                     /locus_tag="PLD67_06095"
     CDS             complement(2104..>3327)
                     /locus_tag="PLD67_06095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOT22124.1"
                     /translation="MHIDVNSAFLSWTAAYEKQMGIERDVRTVPSVIGGNEQSRHGIV
                     LAKSSPAKKFKIQTGMSLMEARQMCPDLLVIPPNYSVYVRASKALREYLKKYTPDVEV
                     FSIDECFLRFTDINREQAVKLAHKIKDGVREKFGYTVNVGISENKLLAKQAGDLEKPD
                     KCHTMFIEEIKEKLWPLPVEELFMVGRRTKPKLNRWGIYTIGELANADYKLISTMLKS
                     HGRLIYNYAWGRDISIFKERAPIKSVGNSSTLRFDVTDRETAHVVLLSLTEMTAWRLR
                     EANMNCRVVSISIKDKDFGFKIKQRKLMYFTDCTRDIYMNACSLFDELWDKKPIRALG
                     VHVSDLEFSEVKQINFFQDKLSLKNEILDKAVDKIRAKYGDNSVIRGVFANSDLKPIL
                     GGYPDDEYPGMSSIL"
BASE COUNT          894 a          718 c          450 g         1265 t
ORIGIN      
        1 catcctgatc tatttcatat cctcgatgta cctcatttgc tgcttcctga agtttcttta
       61 tcaaagtaag tttacccggt gtaaccttca atttctttgc ttcatcaacc tcttttttgg
      121 ttacctctat tacttcaata acattctttt ttctatcctt ttcatttggt ttttcatact
      181 tatttggctt ttcggatttt tcacttcttt cagactcttc tcgtctttca gatttttctc
      241 gcccttccgg ctcctctcgt ctttccgtat cttctttgct ttctgatttg tcccgccttt
      301 caggtctttc tcgattgccg ggtttttctg atttatcatc agtaagttct tcagtttttt
      361 caattgtttc tgtttgctgt gcttcagatt cttccttctc ttctagtttt tccgtacttt
      421 ccggctcctt gggctcttca atttttgttt taatttcatt atctttcgtc tgttccggtg
      481 cttcttcctc attaacggca gttcgaagtt tttcaaacaa cacatctttt tttgcttctg
      541 acttgcttga tgtggcaatt attacgccgc cttcttggcc ttctataata tatcccttat
      601 tttttaattc atttaataca ctttttaccg cgttttgaac ttctttattt ttcaaaccgt
      661 caatatttat atcttttgac aattctcttc catcatcatt aaaacccctc acagtcagaa
      721 ccctgtcgaa tctgtttatc aaaaattcaa aagatggatt tacatctatg cttacatagg
      781 aataaggagt caagtatgcc catgcaggtg taacaaataa catgattgca gcagctgccg
      841 cagccgtcat tctgattaat ttatttctgt tattttttat tactatttct tgacctatgc
      901 aatagtttct gtttttaatt ttagaaacaa caccgtcatc agtcaatact gctgcatgtc
      961 ttcctttaat ttcaactatt attgctttca tttttcaact cctgacgaat atattccata
     1021 taacctgcta aattcggaaa atcccccgac atgatttcta atgctgctat aatatatttt
     1081 cgatggcgtt ccaacaattt acggggtaca tttgtgtatt tttctattat atttaatggc
     1141 aattgcttct taatttttat ttctcttgtc aatattggat tttttattaa ataagccaca
     1201 accttagcgc aggacttctt agttttgtga gattttggcg agcattcggt caaatccata
     1261 aatgaaaatc cataatcgga aaaaatttca ttgatgaggg caatttccat cttaattgaa
     1321 ttattttcaa caatggaaac cttttttgca atttcggact gcaaggagta ttcttcttca
     1381 tcttgagaat cagtattaaa caatgaagga tttactaaaa cttccggcgc atatcttgac
     1441 ttgctgcgaa tgaaatcaat cattctcctt cttattacca attctgcaaa gtttaaaaaa
     1501 ctgcccttat cggcagaata attcttaatt gcttcagtaa aagcagacaa ggctattgac
     1561 cactcatcat cactttttga aatatagctt cgggtagtag atgaggcaca tttaagtatg
     1621 aaattttcat attgaattat taggttattc attaaatctg tattagtctt agcttcaaca
     1681 gccaataggt ccaaatcgct catgttcttc cccttgtagt tacaattaca aaacattata
     1741 tcattcagtc cataatatta caacatttca aatcttacaa aattcttaat ggggaaacaa
     1801 aggaaggggg acacaccttc ctattttgga atagcccctg ccggcaggaa gaattacttt
     1861 ttaaaaagat accatgtctt gctggctacc tcatatttaa gctcaaaaat atccttcccc
     1921 ttatggagac atacaaatac aattcttgtg ttcccgacaa tattttcttc ataagtttta
     1981 attatttttt cgatcctcac aaccttatca tcaagcctga attttaccgg ctctattttt
     2041 ccgtttgtat taaaaacagc cattactttt atgggctcat ttaaaatttt catagtacca
     2101 cctctataaa atactgctca taccaggata ttcatcatcc ggataccccc ctaagatagg
     2161 ttttaaatca ctgttggcaa aaacccccct gataacggaa ttatcgccat acttagctcg
     2221 tattttatct acagccttat ctaaaatttc atttttcaaa cttaatttat cctgaaaaaa
     2281 attaatttgt ttaacctctg aaaactccaa gtctgataca tgaaccccca atgcccttat
     2341 cggcttttta tcccataatt catcaaataa actgcatgca ttcatataaa tatcccttgt
     2401 acagtctgta aaatacataa gctttctttg cttaatctta aaaccgaaat ccttgtcttt
     2461 aatacttatg cttacaaccc tgcaattcat atttgcttct cttaatctcc aggcggtcat
     2521 ttcagtcaaa ctcagcaata caacatgagc agtttccctg tctgtaacat caaatctaag
     2581 ggtactgctg tttcctacac ttttaatcgg ggccctttct ttaaaaattg aaatatccct
     2641 tccccaggca taattgtaaa ttagtctgcc gtgagattta agcattgttg atattaattt
     2701 ataatctgca ttggcaagct ctcctattgt ataaataccc catctgttta attttggctt
     2761 tgttcttcta ccaaccataa atagctcttc aactggcaaa ggccataatt tttcctttat
     2821 ttcctcaata aacatggtat gacacttgtc gggcttttct aaatctcctg cttgttttgc
     2881 aagaagctta ttttcagaaa ttcctacatt tacggtataa ccgaattttt cccgaacgcc
     2941 gtcttttatt ttatgggcaa gtttaacagc ctgctctcta ttgatgtcgg taaatcttaa
     3001 aaaacattca tcaatgctga aaacttctac atcgggagta tattttttca aatattccct
     3061 gagagctttg cttgctctta cataaacgct gtaattggga ggtataacca gtaaatcagg
     3121 acacatttgt cttgcttcca taagagacat tcccgtctgt atcttgaatt ttttggcagg
     3181 acttgatttg gcaagaacaa ttccatgccg gctttgttcg tttcctccta tgacagacgg
     3241 cactgtccgt acatctcttt ctattcccat ttgtttttca tatgcagccg tccagcttaa
     3301 aaatgctgaa tttacatcaa tatgcat
//
LOCUS       DAOSOW010000208         3326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_28755_length_3326_cov_3.782024, whole genome shotgun sequence.
ACCESSION   DAOSOW010000208 DAOSOW010000000
VERSION     DAOSOW010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28755_length_3326_cov_3.782024"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLD67_06100"
     CDS             complement(<1..478)
                     /locus_tag="PLD67_06100"
                     /inference="COORDINATES: protein motif:HMM:NF039791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6470 family protein"
                     /protein_id="HOT22125.1"
                     /translation="MEPLLEIKTIPMAFEMKINRARLELATTDATFEVSRDKGGLQMQ
                     MKPAKIKIDTVEARYSAGLKSAMHLISDVAKKGVQAAYEATATYAREGNLMLDINIMD
                     NPIPEIAMKKFISDVSFNIGFIPSTGPDISWDTGGISMSFAMDKLDFDWNVDKPQIK"
     gene            complement(494..1486)
                     /locus_tag="PLD67_06105"
     CDS             complement(494..1486)
                     /locus_tag="PLD67_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22126.1"
                     /translation="MRITTSMMSNKYIKNLNKSAYEMNYLSEKVETGRKFVKGSEDPV
                     SAIKAYKLRREYRTTEIYDTNIRDVESFLTAAETNLTEISNNMESVYTSYLKGINGTM
                     NDEDREIIAKQLENLQRSILTSLNAKFEDRYVFGGTNKEEIPFTLDMNGNLLFKGLDV
                     NDPANKEALDKLANETINIDLGLGMTFNGDGELNTDTVFDMSMSGIKFMGYGGTDDNP
                     ENLYNLIGKIKDELRNPDFSIDKISPYIDRFEEQKKQVLVHITDIGAKTNYLDFLKTR
                     NEDNQFNLNKKLLEVEFEDPAEAIVNFKMQEYSYNAALSMGNRILQNSFIDFMK"
     gene            complement(1497..3113)
                     /gene="flgK"
                     /locus_tag="PLD67_06110"
     CDS             complement(1497..3113)
                     /gene="flgK"
                     /locus_tag="PLD67_06110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOT22127.1"
                     /translation="MLRSTFLGYKTATSALKVNQNHMDVVGQNISNINTKGYTRQRLD
                     ISSVAFNTSNLKYGTPGPVIGQGVNANGVSQFRDAFLDLRYRMETSKAGSEQVQLEAL
                     RDLESVFDEISMDKLDAQFSDLLTQLHSLTSSPSDPVMEGVVRTSAEILTQMFNDYSR
                     DIETIYNQQLNYLENGAVEKVNELTKNIAKLNQQIKESNISGNPALELNDERNMLVDE
                     LSNYIDIEVDIEPLNIGGGKTIDELVIRLSGTTYELVNRNNSNTISVNEQNGLIKIEL
                     TGATDTDDITNFIKGGQIDGYLKFLNGKGEFATVDDIDPVNGYEKAAEVKGIQYYNNM
                     LDTLANKFATVMNELNRKPDSFDIDGNVQTWITKPLFGARGADPSDPDTEIIRAGNIK
                     ISDDWASATDSYITNTIKEKAGVDEDNTGAVDNILRMINAFNAKNEFDVDRDPSTTND
                     ILFKGTFQEFLSYTTTKLNLQTADVQISFDTYSETQFHIDYARSSMSSVDLNEEGVNL
                     LQYSKSYNAAARLMTTLDEMLETLINRMGV"
     gene            complement(3133..>3326)
                     /locus_tag="PLD67_06115"
     CDS             complement(3133..>3326)
                     /locus_tag="PLD67_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22128.1"
                     /translation="YFKYLRVKITKKLNKINDLLKIVIEVRLRRINNKMNNLGEKENI
                     YTNDKSKNVTEGLLISHKI"
BASE COUNT          883 a          657 c          476 g         1310 t
ORIGIN      
        1 attttatttg aggtttatca acattccagt caaaatctaa tttatccatt gcaaaactca
       61 tggaaattcc tcctgtatcc catgaaatat caggacctgt ggaagggata aatccaatat
      121 taaaactcac atcactgatg aactttttca ttgcaatttc aggtattgga ttatccataa
      181 tattgatatc caacataagg ttgccttccc ttgcataagt tgcagttgct tcataagcag
      241 cttgtacacc ttttttggca acatcactaa tcaagtgcat tgcacttttt aatcctgccg
      301 aatatcttgc ttctacagta tcgattttta ttttggcagg cttcatctgc atttgaaggc
      361 cgcccttgtc tcttgatact tcaaaagtgg catcggtagt tgccagttca aggcgagctc
      421 tattaatttt catttcgaat gccattggaa tagttttaat ttctaatagc ggttccatat
      481 tattttctcc ttgctatttc ataaaatcta taaaactatt ttgcagaatc ctattgccca
      541 ttgataatgc tgcattataa gaatattcct gcatcttaaa gtttactatt gcttctgcag
      601 ggtcttcaaa ttcaacttcc aataattttt tatttaaatt gaattgatta tcctcatttc
      661 ttgtctttaa aaagtccaaa taatttgttt tagccccaat atcggtaata tgaactaata
      721 cttgtttttt ttgctcttca aaccggtcta tgtatggtga aattttatct attgaaaagt
      781 ccggatttct caattcgtcc tttatttttc ctattaaatt atataggttt tccggattat
      841 cgtccgttcc tccatacccc ataaacttta tgcccgacat tgacatatca aatactgtgt
      901 cagtgtttaa ttctccgtca ccgttaaaag tcattcctaa gcctaagtct atgttaattg
      961 tttcattcgc taatttatct aaggcttcct tattggcagg atcattaaca tcaagtccct
     1021 taaataataa atttccattc atatccaatg taaaagggat ttcttccttg tttgttccac
     1081 cgaaaacata tctgtcttca aactttgcat tgagagatgt cagtatggag cgctgcaaat
     1141 tttcaagctg ctttgcaata atttctctgt cctcatcatt cattgttcca ttaatacctt
     1201 ttaaatatga ggtgtaaact gattccatat tgttgcttat ttccgtcaaa tttgtttctg
     1261 cagctgtaag aaatgattca acatctcgta tgttggtatc gtatatctcg gttgttctat
     1321 attctcttct gagcttgtat gcttttattg cagatacagg gtcttcagag cctttaacaa
     1381 acttcctgcc tgtttcaacc ttctcactta aataattcat ttcataagca gatttgttta
     1441 aattttttat atatttattt gacatcatac ttgtagttat tctcatacta tcacccctat
     1501 actcccattc tgtttatcaa tgtttccagc atttcatcca aggtggtcat aagccttgcg
     1561 gctgcattgt atgatttgct gtactgaagt aaatttactc cttcttcatt caaatccacg
     1621 gaagacatgc tgcttcttgc atagtctata tgaaattggg tttctgaata tgtatcaaat
     1681 gatatttgta catcagctgt ctgcagattt aattttgttg ttgtatatga caaaaattct
     1741 tggaatgtac ctttaaacag tatatcattt gtggtgcttg ggtctctgtc aacatcaaat
     1801 tcattcttag cattaaatgc atttatcatt cgaagtatat tgtcaacagc tccggtatta
     1861 tcctcatcta cgccagcttt ctccttaata gtgttggtta tatatgagtc tgttgcactt
     1921 gcccagtcat ctgatatttt tatgttgccg gctcttatga tttctgtatc agggtcagat
     1981 ggatctgcac ctcttgctcc gaatagaggt tttgtaatcc atgtctgtac atttccgtct
     2041 atgtcaaatg aatccggctt cctgttcagc tcattcataa ctgttgcgaa tttatttgct
     2101 aatgtgtcta acatattatt ataatattga atacctttaa cttctgctgc cttttcatat
     2161 ccattaaccg ggtcaatatc atccaccgtg gcaaattcgc cctttccgtt aaggaacttt
     2221 aagtaaccgt ctatttgacc gccttttata aaattggtaa tatcgtccgt atctgttgca
     2281 cctgttaatt ctattttaat taatccattt tgctcattaa cagaaattgt gttgctatta
     2341 tttctattaa ccaattcata tgttgtcccg cttaatctga taaccaactc gtctattgtc
     2401 ttgcctccac ctatattgag aggctcaata tctacttcta tatctatata gttggaaagc
     2461 tcatctacca gcatatttct ttcatcatta agttccaatg ccggattgcc gcttatattg
     2521 ctttctttaa tttgctgatt taatttggct atgttttttg tcaactcatt tactttttca
     2581 acagctccat tttccagata attaagttgt tgattgtata tagtttctat atctcttgaa
     2641 taatcattga acatttgagt aagaatttct gcagatgttc tgacaacacc ttccattaca
     2701 gggtctgaag gactgcttgt cagtgaatga agctgtgtta aaaggtcgga aaattgcgca
     2761 tctaatttat ccattgatat ttcatcaaac actgattcca agtctctcaa agcttctaat
     2821 tgaacctgct cgctgcctgc ctttgaagtt tccattctat atctcaaatc aagaaatgca
     2881 tctctaaact ggctcacacc gtttgcatta actccttgtc ctattacagg tcccggagtg
     2941 ccatacttta aattgcttgt gttaaaagca acagaactta tgtcaagacg ctgcctggtg
     3001 tatcccttag tatttatatt cgaaatattt tgccccacta catccatgtg gttttgattt
     3061 acttttaatg cacttgttgc tgttttatat cctaaaaaag ttgacctgag catagcttct
     3121 cctaactttc ttttatattt tatgtgaaat caataaacct tcggttacat ttttgctttt
     3181 gtcatttgta tatatatttt ctttttctcc taaattgttc attttattgt ttatcctgcg
     3241 aagtcttact tcaataacaa tctttaataa atcgtttatt ttatttaatt ttttagtaat
     3301 ttttaccctt aaatatttaa aatatg
//
LOCUS       DAOSOW010000209         3325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_28769_length_3325_cov_2.888991, whole genome shotgun sequence.
ACCESSION   DAOSOW010000209 DAOSOW010000000
VERSION     DAOSOW010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28769_length_3325_cov_2.888991"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="PLD67_06120"
     CDS             <1..996
                     /locus_tag="PLD67_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22129.1"
                     /translation="KVVGIDISSNCLNNILEQNIELNNHILDSKDAYAFVNTHFDKIL
                     FSVINKGNDFFMISNIERNHGNMVETDHRHDRDDMVIRVEYINKCCRYYHSVNNKKVN
                     RIYLYGYNSSDGLDKLLSSILNLEVEYINSVSNISIADNISINEYFISLTALYNAKSS
                     FLTKNEKRIQILINFAGASVILFCLLFFLSKHSNSVRKQIAYLDIYIKNEKNIERNLI
                     IEKLKSEIRLLNSAITSIETAMDLVNNENVRTENIKGVYNSLPGNTEVLSFSIDKDSL
                     NMQCISDSMDEVLCLLRNLEGLYFVESVYIPAIQSMKDMKYSYTIICNFKVNYEG"
     gene            986..1537
                     /locus_tag="PLD67_06125"
     CDS             986..1537
                     /locus_tag="PLD67_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22130.1"
                     /translation="MKDKYLVFIIVLFVIAILYGLWPLYCKFNPAQNKLNTSVEAYKS
                     MMDNFRSEDSIKSKKIDYINKINKSDFRSQLSQEKIISVLYTCALKNNIRISDIKFVE
                     TDDAPDGDIDIIESKEDFVFETRCLIAEFKAGLGQLLQFIDEIKSFRDISVINLDVSI
                     WEEDIVFAFVHMKFYAVPVDMNL"
     gene            1593..1979
                     /locus_tag="PLD67_06130"
     CDS             1593..1979
                     /locus_tag="PLD67_06130"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOT22131.1"
                     /translation="MNKLFNNRGLTLAEVIMTMAILGIVICPLMNLLVLSQNINSEGE
                     KEYDVIQTAQYYMEETRAMGEIDPEIFVYNSEEMSYERMVSDVLDDLSAEIKITPFSY
                     GLHYIEVNILNDGEVINSLTGSIVID"
     gene            2070..2609
                     /locus_tag="PLD67_06135"
     CDS             2070..2609
                     /locus_tag="PLD67_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22132.1"
                     /translation="MNNKGSTLVLLIIVIALVIVLGASVLNIAVKQYAIKKFNIDSKQ
                     AFYFSETGLNEAYVKSCALIEESIIKALQITEDYISINSFNEQAENIFVTSYKIYIGT
                     NIENRIETASNPKVKVWNDTLVFIDNALTLELKSSYIHNDIDKVTGVELVIGVPDYHD
                     VSEGSYDVRDYIKFKNWNS"
     gene            2733..3260
                     /locus_tag="PLD67_06140"
     CDS             2733..3260
                     /locus_tag="PLD67_06140"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOT22133.1"
                     /translation="MKNKGFSLVELLVSLGISSIIIGLIFSFFIINFKGYKSVRNDFE
                     MQFQAQYILNFMAGRIVDSNSVSFARVNTQNYSMTAVRSAGAEYPIHKISFKYGSESE
                     NYVFHIINNTIRYGKGEKEMNPTVELGNYVDEMYVSLFKDESFQDAKAVKLKLVMKKD
                     EQLHEAFQAVYMRNN"
BASE COUNT         1174 a          427 c          615 g         1109 t
ORIGIN      
        1 aaagtcgttg gcatagacat atcttctaat tgcctcaaca atatacttga acaaaatata
       61 gaattgaata atcatatttt ggactctaaa gatgcgtatg cttttgttaa tactcatttt
      121 gataaaatat tgttctctgt cataaataaa ggtaatgatt ttttcatgat ttcaaatata
      181 gaaagaaatc atggcaatat ggttgaaact gaccataggc atgaccggga tgatatggtt
      241 atccgggttg aatatataaa caaatgctgc aggtattatc attccgtaaa taacaaaaaa
      301 gtaaacagaa tatatttgta cgggtataat agctctgatg gtttggacaa gcttttgtca
      361 tccattctga atctggaagt cgaatacata aactctgttt ctaatatctc aattgctgat
      421 aatatcagta taaatgagta tttcatatct ttaacggcat tgtataatgc taaatcatca
      481 tttctgacaa aaaatgaaaa aagaatacaa attttaataa actttgccgg agcatcggtg
      541 atactttttt gtttgctttt ctttttatca aagcattcaa actcagtcag gaaacagatt
      601 gcatatttag acatttacat taaaaatgaa aaaaatattg agagaaatct tataattgaa
      661 aaactaaaat cagaaatccg attattaaat tcagccataa catctattga aacagctatg
      721 gatttggtta acaatgaaaa tgtcagaact gaaaatataa aaggagtata taattcactc
      781 cccggcaata ctgaggtttt atcattttca attgataagg atagtttaaa tatgcagtgt
      841 atatctgatt ccatggatga agtactctgt ttgttgagaa atttggaagg attatatttt
      901 gtggaaagtg tttacattcc tgcaattcaa tcaatgaagg acatgaagta ttcatacacg
      961 ataatttgta atttcaaggt taattatgaa ggataaatat ttagttttta ttattgtatt
     1021 atttgttata gctatacttt acggcttatg gcccttgtac tgtaaattta atccggctca
     1081 aaacaagctg aatacttctg ttgaagctta taaatccatg atggacaatt ttcggtcaga
     1141 ggattctatc aagtctaaaa agattgatta cataaataaa ataaacaaat cagatttcag
     1201 gtctcagtta agtcaggaaa aaattatttc tgttttatat acctgtgcat tgaaaaacaa
     1261 tattcgtatt tcagatatta aatttgtaga aacagatgat gctccggatg gagatattga
     1321 tattatagaa tcgaaagagg attttgtttt tgaaaccagg tgcttaatag cagagtttaa
     1381 agccggattg ggacaacttt tgcagttcat agatgaaatt aaaagtttta gagatatcag
     1441 cgtaattaat ttagatgtat caatatggga ggaagatatt gtttttgcat ttgttcatat
     1501 gaagttttat gcagttcctg tagatatgaa tttatgaagc agaacatttt gttttttgca
     1561 gcgtccggga aaaattcgta tggggtggta ttttgaataa attatttaat aacagaggat
     1621 tgaccttggc agaggtaatt atgacaatgg caatacttgg aattgtaatc tgtcctctga
     1681 tgaatttatt ggttttgtcg cagaatataa acagtgaagg tgaaaaagaa tacgatgtaa
     1741 ttcaaacagc tcaatactat atggaagaaa caagagcaat gggtgaaatt gacccggaaa
     1801 ttttcgttta taacagtgaa gaaatgtctt atgaaaggat ggtttcggac gttttggatg
     1861 atttaagcgc agaaatcaaa attaccccct ttagttacgg actgcactat atagaagtta
     1921 atattttaaa tgatggagaa gtaattaaca gcttaacagg gagtatagtt atagattgat
     1981 gagatccttc gcttatgctc aggatgacaa ccctttgtgt cattctgaga gctttagctc
     2041 gaagaatctc atgtttaaca ggagtatgta tgaataataa aggttctacc ttagtacttt
     2101 tgataattgt aattgcactg gtaattgttt tgggagcttc cgttttgaat atcgcagtta
     2161 aacaatatgc aataaaaaag ttcaacattg attccaaaca agcattttat ttttctgaaa
     2221 ccgggctcaa tgaagcctat gtcaaatcat gcgctttaat tgaagaatca attattaaag
     2281 cattgcaaat aactgaagat tatatatcaa taaattcatt taatgaacaa gcagaaaata
     2341 tttttgttac aagttataaa atttatatag gaacaaatat tgaaaataga attgaaaccg
     2401 catccaatcc taaagtaaaa gtatggaatg atactttggt ttttattgat aatgcactta
     2461 cgttagaatt gaaatcttct tatattcata acgacattga taaggttacg ggagttgagc
     2521 tggttatcgg tgtgccggac taccatgatg tatcagaggg ttcctatgat gtaagagatt
     2581 atataaaatt taagaattgg aacagttaaa agctgagatc cttcgctatc gctcaggatg
     2641 acaatttcgt catttttgtt aataattggc tttaagggtc aagtcatcgt cattctgaac
     2701 acagtaaaga atatcatata aaaggggttc ttatgaaaaa taagggtttt tcattggttg
     2761 aacttttggt gtcattaggg atatcctcta ttattatagg gttaattttc tcttttttta
     2821 taattaattt taaaggttat aaatcagtga gaaatgattt cgaaatgcaa ttccaggcac
     2881 aatatatatt aaactttatg gccggaagga ttgttgattc taattctgtg agttttgcaa
     2941 gagtaaacac gcaaaattat tccatgactg ctgtaagaag cgccggagca gaatacccca
     3001 tacacaaaat ttcttttaaa tatggaagtg aaagtgaaaa ttacgtattt catattataa
     3061 acaacaccat tcgctatggg aagggagaaa aggaaatgaa tccaacagtt gagcttggca
     3121 attatgtgga tgaaatgtat gtttcattat ttaaggatga aagttttcaa gatgcaaagg
     3181 cagtaaaatt aaaactggtt atgaaaaagg atgagcagtt gcatgaagct tttcaagcgg
     3241 tttatatgag aaacaattga gattccggat tgtcatcctg agcgatagcg aaggatctca
     3301 taaggcccga ctaaatttat ataaa
//
LOCUS       DAOSOW010000210         3306 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_28985_length_3306_cov_3.601353, whole genome shotgun sequence.
ACCESSION   DAOSOW010000210 DAOSOW010000000
VERSION     DAOSOW010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28985_length_3306_cov_3.601353"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1702)
                     /locus_tag="PLD67_06145"
     CDS             complement(143..1702)
                     /locus_tag="PLD67_06145"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT22134.1"
                     /translation="MKKIIIIVILCVLFSGCSTFFSDVTEIEAVNLQNGVKIVLPKDS
                     KDADIIIDAVNKKNKTDEDITSLLSYEIILKKENDEEVYKLYFDLDNQTAYLAQDKTV
                     FKIRDNIAKQLFLSDAFSYIYISAMYNSYIEVNGQQINPDIEYNWNSRNIEGDFVNNA
                     GLLTGKYEENEKITVSHSDSIDIKHEKQPDSQIIRIYEQGSLFGTGKNLSELISNIKS
                     DGEYLVECESQWHFKENRESYGSQKLKFSISVDIPAQINIVTKENYPGNILLISIENL
                     NDDETVKLETDAVKTNFDLYAYKDKNYYMMPIDLYTQPGEHNVIAVVNEGAEDEYTLE
                     KTLTVSNRSFKTQYLTVSEEMNETHNDNAAIYEFAQLVKPARTESAKEKLWEGTFIMP
                     VEGELTTDFAEIRYVNNEISSSRHSGLDLAAPKGTPVVAPNNGRVTFAMEGLLSPGNT
                     VVIDHGMGLFTSYYHLDTINVKKGDEVKKGDVIGTVGTTGFSTGPHLHYAVSIYNTYV
                     NPYQTLSGIID"
     gene            complement(1946..2896)
                     /locus_tag="PLD67_06150"
     CDS             complement(1946..2896)
                     /locus_tag="PLD67_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT22135.1"
                     /translation="MFIKFLIAAVFAGTPLLFGTVGEIMNEKAGHLNLGVEGMMAMGA
                     CAGFMAGFFSNSIYIALLAAFLAGMLGALIYAVLTITFMANQNVTGLTLSIFGVGISN
                     FAGEFMLVNSASNSLKLPEQITAQISNINIPGISTIPVLGPLLFQYNIFVYLGVVIAV
                     TCGIYYSRTKTGLNVRAIGENPGAADAAGIKVTKLKYINVLIGGGICGIGGAYCSMII
                     NGGVWMNNPVNGQGWIAVALVIFASWKPLRAIFGSFIFGAFNILKYYFPKTIFTIPNA
                     FYDMLPFAITALVLVVTSIRKSKENQQPASCGVNYFREER"
     gene            complement(2898..>3306)
                     /locus_tag="PLD67_06155"
     CDS             complement(2898..>3306)
                     /locus_tag="PLD67_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT22136.1"
                     /translation="GYEISVVGESKSTARYAGMDVKKIILRTMIISGGICGITGMIQA
                     AGSDMTLASSVAGGVGFTAIIVSWLAYLNPFGILLVSFLFSILDKGSSVMQSTYGLST
                     YFASVLQGIILFFILGCEFFIRYKFVLTKKGGK"
BASE COUNT         1025 a          674 c          496 g         1111 t
ORIGIN      
        1 actactggga cattacttta tggactcact ccaacgaatt acataccatg aaggtgtgtt
       61 ccccctccta tattaagttg tatccccctt ctaataactt tacattaatt atataccaca
      121 attcaaacct ggtaaacagt tttcaatcaa ttatcccgct taaggtttga tatggattta
      181 cataagtgtt ataaatactc actgcataat gcaagtgggg cccggttgaa aatcctgttg
      241 tacccacagt tccaataaca tccccctttt taacctcatc gccttttttt acattaattg
      301 tatctaagtg atagtaggaa gtaaaaagcc ccattccatg gtcaataaca accgtgttgc
      361 ctggagaaag aagtccttcc atagcgaatg taaccctccc gttatttgga gctacaacag
      421 gagttccttt tggagcggct aagtcaagac ctgagtgccg cgaagaagaa atttcattat
      481 tcacatatct tatttcagca aaatccgttg tcagttctcc ttcaacaggc attataaatg
      541 taccctccca caacttttcc tttgccgact cggttcttgc aggttttaca agctgtgcaa
      601 attcatagat tgcagcatta tcattatgtg tttcgttcat ttcttcgcta actgtaagat
      661 attgagtttt aaaacttcta tttgacacag tcaatgtttt ttccaatgta tattcatctt
      721 ctgccccttc atttacaact gctataacat tatgttcacc gggctgtgta tacaggtcta
      781 ttggcatcat ataatagttt ttatctttgt atgcataaag atcaaagtta gttttaactg
      841 catctgtttc caattttact gtttcatcat catttaaatt ctcaatacta atcaacaaga
      901 tgttgcccgg ataattctcc ttggttacta tattaatttg tgcaggtatg tctaccgaga
      961 tagaaaattt caacttctga cttccgtatg actctctgtt ttccttaaag tgccactggc
     1021 tttcacattc aaccaggtat tcaccgtcac tttttatatt acttatgagc tcagacaaat
     1081 tttttcctgt gccgaacaaa ctgccttgct cataaattct tattatttgg ctatcaggct
     1141 gcttttcatg tttaatatct attgagtcgg agtgtgacac tgttattttt tcattctctt
     1201 catattttcc cgttaatagt cctgcattgt ttacaaaatc tccttctata tttctgctgt
     1261 tccaattgta ttcaatgtca ggatttattt gttgcccgtt tacctcaata tacgaattat
     1321 acattgctga aatatatatg tatgaaaaag catcgcttaa aaacaattgt tttgcaatat
     1381 tgtctctaat tttaaacact gttttatcct gagcaagata tgcagtttga ttgtctaaat
     1441 caaagtaaag cttgtaaact tcttcatcat tttctttttt tagtataatt tcatagctca
     1501 ataaggatgt tatatcttca tctgttttat tcttcttatt tactgcatct ataattatat
     1561 ccgcatcctt gctgtctttt ggtaaaacaa tcttaacacc gttttgtaaa tttactgctt
     1621 caatttcggt aacatcggaa aagaaagtgc tgcaaccgga aaataaaaca cacaatataa
     1681 caataattat tattttcttc ataatacctc catcaagtag tttttgattt tgacaaagta
     1741 ttatacatta ttatatggaa attgaactat gaaaatacaa aataacacaa taatagtata
     1801 acatagatat gctgatatga aataaaaaaa tgcgatatat ttaaagagct gtcaaatgac
     1861 agctctaatt tcaacagatg agattattgg agctaaggct ctcagaatga cacatagctt
     1921 attcatagca atacctaagg tcatgttatc tttcttctct gaaataattt actccgcagc
     1981 ttgcgggctg ttggttttcc tttgatttcc taattgaagt tactaccaat accaaggctg
     2041 ttatagcaaa tggaagcata tcataaaatg catttggaat tgtaaaaatt gtttttggaa
     2101 aataatattt taaaatattg aatgctccaa atataaagga gccgaaaatt gctcttaagg
     2161 gtttccatga agcaaatata acgagagcaa cggcaatcca tccctgaccg tttaccggat
     2221 tgttcatcca tacaccgccg ttgataatca tggagcagta tgctcctcct ataccgcata
     2281 taccgccgcc aatgagcaca ttaatatact ttagctttgt taccttgatt cctgctgcat
     2341 cagctgcacc gggattttcc ccgatagctc tcacattgag ccctgtcttt gtgcgagagt
     2401 agtatatacc gcatgttaca gcaataacta cacccaaata tacaaaaata ttgtattgaa
     2461 ataataatgg tcccaaaaca ggaatagtgc tgatgccggg aatatttata ttgcttattt
     2521 gtgcagttat ttgctcagga agctttaaag aatttgaagc agaatttaca agcataaatt
     2581 ctcccgcaaa gtttgatata cctacaccga aaattgaaag ggtaagaccg gtaacatttt
     2641 gatttgccat aaatgtaatt gttaaaacag cataaattaa agcacccagc atgcctgcca
     2701 aaaaagctgc caaaagggca atataaatac tattgctgaa gaatcctgcc atgaatccgg
     2761 cacatgctcc catagccatc attccttcaa ctccaagatt taaatgtcct gccttttcat
     2821 tcataatctc gcctaccgta ccaaataaca gaggagttcc cgcaaatacc gcagcaatta
     2881 aaaatttaat gaacatgtta tttccctcct tttttggtaa gtacaaattt atatcttatg
     2941 aaaaattcgc atcctaatat aaaaaacaga attataccct gcagcacact tgcaaaatat
     3001 gttgacaatc cataggtaga ctgcatgacg ctgctaccct tatcaagtat gctgaacaaa
     3061 aatgaaacca aaagtattcc aaaaggattt aaatatgcca gccaggatac aattattgca
     3121 gtaaatccaa ctcctcctgc tacagaagat gctaaggtca tgtctgaacc ggcagcctga
     3181 atcattcctg taataccgca aattccgccg cttataatca tggttcgaag tattattttt
     3241 tttacatcca tgcctgcata tctggcagta gatttacttt caccgactac gcttatttca
     3301 tatccg
//
LOCUS       DAOSOW010000211         3288 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_29210_length_3288_cov_4.420353, whole genome shotgun sequence.
ACCESSION   DAOSOW010000211 DAOSOW010000000
VERSION     DAOSOW010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29210_length_3288_cov_4.420353"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PLD67_06160"
     CDS             <1..225
                     /locus_tag="PLD67_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017687308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT22137.1"
                     /translation="RNMENEVDLSLLNDSIQKLNKREYMIMKLRFGLNNTKSFTQKEV
                     ADILGISQSYISRLEKKILNRLKKDINKAV"
     gene            289..1068
                     /gene="sigG"
                     /locus_tag="PLD67_06165"
     CDS             289..1068
                     /gene="sigG"
                     /locus_tag="PLD67_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HOT22138.1"
                     /translation="MINKVVICGVNTSQLPTIKTSEMRGMIKKIQEGDTELREKFIKG
                     NLRLVLSVIQRFNSRNEPVDDLFQIGCVGLIKAIDNFDLSLDVKFSTYAVPMIIGEIR
                     RYLRDNNSIRVSRSMKDTAYKALQVRERLTAENSQEPTIAEIAKEVGVDKEEIIFALE
                     SIQEPVSLFEPIYNDGGDALYVVDQVRDEKNIDEKWIEKITLEDSINNLSEREKQIVD
                     MRFYKGKTQMEVADEIGISQAQVSRLEKSALNQLKKGFKTS"
     gene            1335..1721
                     /locus_tag="PLD67_06170"
     CDS             1335..1721
                     /locus_tag="PLD67_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT22139.1"
                     /translation="MIIKRYSDVDESPLFDMLKEEGDDWSDYHGEKGRDKYIKALKSS
                     LTYVIYDDNILCGFVRCREDDGFGVYVYDLLVRKSYRGRQLGKKLMEQVCKDFANQPV
                     YVMSDVDPYYEKLGYRREGSIFEVKI"
     gene            1821..2093
                     /locus_tag="PLD67_06175"
     CDS             1821..2093
                     /locus_tag="PLD67_06175"
                     /inference="COORDINATES: protein motif:HMM:NF017088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HOT22140.1"
                     /translation="MTNYWDIAEKDVVNIKNGEVMGRFDDVEIDTRKGKITSFYIEEG
                     SKFMGMLGKTKPRRIRWEEILKIGADVIIVNVDDDIHNKAIKDMLD"
     gene            complement(2141..3286)
                     /locus_tag="PLD67_06180"
     CDS             complement(2141..3286)
                     /locus_tag="PLD67_06180"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOT22141.1"
                     /translation="MLGSTGSIGTQTLDVVREHSDKITVCAISGNNNIELLLKQIIEF
                     TPELCCVMDDSNALKLKELLPPHIKTEIVSGMEGLIAIAEYEKSEIIVTAVSGMIGLK
                     PTVAAIKKGKSIALANKETLVTGGSYIINLSKKHNAKIFPVDSEHSAIFQCLMANENK
                     AVNKILLTASGGPFRGKNEEFLKTVTVEDALKHPSWSMGKKITIDSATLMNKGLEVIE
                     AKFLFDIEPEQIEVIVHPQSIIHSGVEFIDHSTIAQLGLPDMRIPIQFALFYPRRMRN
                     NYKSLSLTDIGTLTFEKPDLSTFKCLSLAFEALSAGGTMPAVLNASNEACVKLFLDKK
                     ISFLDIGNINEKVMLSHTPAKIDSIETILEAEEFAKQMVINLTKGRG"
BASE COUNT         1087 a          495 c          690 g         1016 t
ORIGIN      
        1 cgtaacatgg aaaatgaagt ggatttaagt cttcttaatg attcaattca aaaattaaat
       61 aaaagagaat atatgattat gaaattaagg ttcggtctaa ataataccaa gagtttcaca
      121 caaaaggaag ttgctgatat acttggaatt tcacagtctt acatttctcg cctggaaaaa
      181 aagatactaa acagactaaa aaaggatatt aacaaggctg tataaaaagg tacagtcgag
      241 gatataattt taaaggggga ataatttctt taaaggggaa aaacgacgat gataaacaaa
      301 gtagttatat gtggcgttaa tacttctcag ttgcccacca taaagacatc agaaatgaga
      361 ggtatgatta aaaaaatcca agaaggagac actgagctta gagagaagtt tatcaaaggg
      421 aatttaagac ttgttctaag cgtaattcaa agatttaatt caagaaatga gcctgttgac
      481 gatttgtttc aaattggttg cgtcggactg ataaaggcta ttgataattt tgacttgtct
      541 ttagatgtaa agttttctac ctatgctgtc cccatgataa taggggaaat cagaagatat
      601 cttagagaca ataattccat aagagtatcg aggtcaatga aagatactgc atacaaagca
      661 ttgcaggtaa gggaaagatt aacggctgaa aattcacagg agcccacaat tgctgaaatt
      721 gcaaaagagg tgggagttga taaggaagaa atcatatttg ccttggagtc tatacaagag
      781 cccgtatcct tgtttgaacc tatttacaat gacgggggag atgctcttta tgttgtagac
      841 caggtaaggg acgaaaagaa tatagatgaa aaatggattg aaaaaataac tttggaagat
      901 agtataaaca acttatccga aagggaaaag caaattgttg atatgcgttt ttataaaggg
      961 aagacgcaaa tggaggtggc ggatgaaatc ggaatttccc aagcgcaagt atcccgcctt
     1021 gaaaaatcag cactgaacca gttgaagaag ggattcaaaa cttcataata tgccataaac
     1081 tcgtatttgt aaatttgagg aacgtacagc gttccttttt tgtgtcatgg gatggagata
     1141 ttacacatat aaagaatttg ttgattctaa ttatattata aagtaaaatt agttaaacga
     1201 taataacaat actttcgcaa aaggggatat tcctcgccaa atattatata gaattttcac
     1261 ggagaaatta tgaatgttgt tgtcatcatg cagcagacaa caatcaggtc ttggtgagat
     1321 taaaggagaa agaaatgatt ataaaaagat attctgatgt tgatgagtca cccttgtttg
     1381 atatgctaaa agaagagggg gacgattgga gcgactatca tggagaaaaa ggccgtgata
     1441 aatatattaa agcattgaaa tcaagcctta cttatgtaat ttatgacgat aatatcctgt
     1501 gcggctttgt tcgatgtcgt gaggatgatg gttttggtgt ctatgtctat gatttacttg
     1561 tgagaaaatc ctatcgtggg agacagcttg gtaaaaagct tatggaacaa gtgtgcaagg
     1621 attttgcaaa tcaacccgtt tatgtgatga gcgatgtaga cccttattat gaaaagcttg
     1681 gttaccgaag agaaggttcg atttttgaag tcaaaatata agtaacttgc tgtataccct
     1741 tatggtgatt tgctacaatg tttgtcatat ttgataactg ttgtacatat tttattataa
     1801 aagggtaagg tggtgatgtt ttgactaatt attgggacat agctgaaaag gatgttgtta
     1861 atataaaaaa cggcgaggtt atgggcaggt ttgacgatgt agaaatagat acaagaaagg
     1921 gcaaaattac atccttttat atagaggaag gaagtaagtt tatggggatg ctcggcaaga
     1981 caaaacccag aagaatcaga tgggaagaaa tactaaaaat cggcgcggat gtaataattg
     2041 ttaacgttga tgatgatata cataacaaag caataaaaga tatgttggac tagacgacaa
     2101 ggggacaatg acaaggggac gggggctatg ttatacgaca ttaaccccgt ccttttgtca
     2161 aattgattac catttgtttg gcaaactctt ctgcttcgag tattgtttct attgaatcga
     2221 tttttgcagg ggtatggctt aacataactt tttcgtttat gtttcctata tcaaggaaac
     2281 ttattttttt gtccaaaaac aatttgacgc atgcttcgtt cgatgcattt aaaactgcag
     2341 gcattgtacc gcccgcactt aatgcttcga aagctaaaga taagcatttg aatgtagata
     2401 aatccggctt ttcaaatgtt aaggtaccta tgtctgttaa ggacaggctt ttataattgt
     2461 ttctcatacg tcttggataa aacagcgcaa attgtatggg gattcgcata tccggaagtc
     2521 ccagctgtgc aattgttgag tggtcgataa attcaactcc cgaatgtata atgctttgag
     2581 ggtgcacaat aacttcgatt tgctccggct caatgtcaaa caagaatttt gcttcgatta
     2641 cttccaaacc tttgttcatt aatgtggcgg aatctatggt tatttttttg cccattgacc
     2701 aactgggatg tttcagggca tcttcgacgg taacagtttt taggaattct tcatttttgc
     2761 ctctgaaagg accgcctgaa gcagtcagca atattttatt gacagctttg ttttcatttg
     2821 ccatcaggca ttggaaaata gcgctgtgct cactgtcaac ggggaaaatt ttagcattgt
     2881 gtttttttga taaatttatt atataacttc cgcctgttac caatgtttcc ttattggcaa
     2941 gggctattga ttttcctttt tttattgcag caactgtagg cttaagacca atcattccgg
     3001 atactgcagt aacaataatt tcgctttttt catattctgc gattgcaatt aaaccttcca
     3061 tgcctgaaac aatttccgtt tttatatgag gtggcagcaa ctcttttaat ttcagcgcat
     3121 tactgtcatc catcacacag cagagctccg gagtaaattc aattatttgc ttcaataaaa
     3181 gctcaatatt gttatttccg gaaattgcac aaactgtaat tttatctgaa tgttcacgta
     3241 caacatctaa ggtttgtgtt ccgattgaac cggtggaacc taaaatac
//
LOCUS       DAOSOW010000212         3274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_29367_length_3274_cov_2.230817, whole genome shotgun sequence.
ACCESSION   DAOSOW010000212 DAOSOW010000000
VERSION     DAOSOW010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29367_length_3274_cov_2.230817"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..490)
                     /locus_tag="PLD67_06185"
     CDS             complement(59..490)
                     /locus_tag="PLD67_06185"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HOT22142.1"
                     /translation="MRLKHFKKENGQAMVEFALVLPILLLFIAGILDFGWIYHNQLSA
                     NNASREAARYIAIHYYFDNMDSASATSKASNIINEYTNIADIVVDTIIPEADDVNGGE
                     RIRVRFAGKVIILTPITRAIIDKDGDGKFPIRAETSMRVEK"
     gene            complement(569..748)
                     /locus_tag="PLD67_06190"
     CDS             complement(569..748)
                     /locus_tag="PLD67_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOT22143.1"
                     /translation="MKKLFSWLKNEESGQGMVEYGLILALIAVVVIVALRTIGTNANA
                     TFENIADELEGTATP"
     gene            complement(1024..1191)
                     /locus_tag="PLD67_06195"
     CDS             complement(1024..1191)
                     /locus_tag="PLD67_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HOT22144.1"
                     /translation="MKKIAACILKEEDGQGLVEYSLILSLIVLAAVAAVSFLGENAFA
                     LYQDSMDKMSP"
     gene            complement(1213..1878)
                     /locus_tag="PLD67_06200"
     CDS             complement(1213..1878)
                     /locus_tag="PLD67_06200"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOT22145.1"
                     /translation="MLYKLIVFLTLGLFTGYIIPKLSLKLIEYKEKTKNIDVNKAQPS
                     GSRINILIILFNGAAWCFTAYTQESLFTAVLICIQISLGLIFAYIDIKIRIIPNELIL
                     ILLTLGITYQAFSYGLEGILGSVICMIFIMIVFTALATFMGLGKVGAGDVKLAGAIGF
                     ALGYPSVVTAMGAMAAVLLGYILIGLALRKIRLTTMLPLAPFLVAGYIFALVTFTVNL
                     NIA"
     gene            complement(1928..>3274)
                     /locus_tag="PLD67_06205"
     CDS             complement(1928..>3274)
                     /locus_tag="PLD67_06205"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOT22146.1"
                     /translation="MINTIGITKLHSYEIRNSKLDNIAKVTVILALSVIPAVFVPLGG
                     TTDHFYLPKVAAMIILALSFLAVLAINKIRFKDIVQKDRINISLFIYFILLAASVYAA
                     ENKVFAIIGVPGRWEGLVTITLYMFLFITARLYLVPDEGLFKIILVTAIIVSIYGILQ
                     TMNFDPFPRDVLRENWSKRAFSTMGNPNFLGSYIVLIIPTTIFFYIINKNITGLTAYA
                     ILFYCLLSTGTRGAWLGTIASIMAFAAIHYMYFRYSKGEFTRYIILLVITILLLALYN
                     FNTEGAFIDRFLSIKRDADEFLTKGDRADYSGANRGFIWKRVAELIKKRPLTGYGIEN
                     LGEAFKKYYTQDMIELWNEVRYLDKAHNEYLHIAVTSGIPSLLVYLTFISQIILKGLF
                     RLKNCKTALLILSSVIGYMTAAFFNISVVSVAYVYWIFLGLLAGSNGNCYKFNLRA"
BASE COUNT         1038 a          648 c          495 g         1093 t
ORIGIN      
        1 cattttttaa cttaactaaa tatttagata tattcataat atcaccgccc ttcatacttt
       61 atttttctac tctcatagat gtttcagctc ttatgggaaa cttaccgtct ccatccttat
      121 ctattattgc tctggttatt ggagtaagta taatgacttt tcctgcaaac ctgaccctta
      181 ttctttcacc accattcaca tcgtcagctt caggtattat ggtatctaca actatatcgg
      241 caatattggt atattcatta ataatatttg aagccttact tgttgcactt gcagaatcca
      301 tattatcaaa ataatagtgt atggctatat atctggcagc ctcacggctg gcattattgg
      361 cagatagctg attatgataa atccatccga aatcaagaat tccagctata aaaagcaata
      421 gtatagggag taccaaggca aactcaacca ttgcttgacc attttctttt ttaaaatgct
      481 taagtctcat aattttaccc cccattgtgg aaaaagcacc gtcccgacca tgcggacgag
      541 ccggtgctta gctggttttt tggttaaatc aaggtgtagc tgtgccttcc aattcgtctg
      601 caatattctc gaatgttgca tttgcatttg taccaatagt tcttagtgcc actattacta
      661 ccacggcaat caatgcaagt attaagccat actcaaccat tccttggccg ctttcctcgt
      721 tttttaacca actaaataat tttttcattt taaccccctt gaattttatt tatattttga
      781 tataaaatat cataatatac aattttttgc ggtgtcatcc tttgagattc ttcacctttg
      841 gttcagatat gattgtcatc ctgagcgtaa gcgaaggatc tcaggttttg agacttcgca
      901 gtctcattca ccaatttatt tgttccttgc gtagcataaa ttgggaattc gttgcgaata
      961 acactaacgt taagtttatt catagcaatg atacggtacc attacaataa taatagatac
     1021 caattacggt gacattttat ccattgaatc ttgataaagt gcgaatgcat tttctcccaa
     1081 aaagcttact gcggcaacag ctgcaaggac tatgagggat aatatcaaag agtattcaac
     1141 taatccttgt ccatcttctt cttttaaaat acatgcagca atttttttca tatccgtatc
     1201 ctttctttaa cattacgcaa tgtttaagtt aacagtaaat gttactaatg caaaaatgta
     1261 acctgctact aagaagggag ctaagggtag catagttgtc aggcgaattt ttcttaaagc
     1321 cagtccaatc agtatatagc ctaacaatac tgctgccatt gcacccatag ccgtaactac
     1381 ggagggatat ccaagggcaa atcctatggc tcccgctaat ttcacatcac ctgctccaac
     1441 cttgcctaat cccataaagg ttgccagtgc agtaaacaca atcataataa aaatcataca
     1501 aattactgag cctaagattc cttccaaccc ataactaaat gcctgatatg tgatgcctaa
     1561 ggttaataat attaaaatca actcattagg aattattctt atttttatgt cgatatatgc
     1621 aaatattaat cctaatgata tttgtataca tatgagtact gcagtaaata agctttcttg
     1681 tgtataagcc gtaaaacacc atgctgcccc gttaaacagg attatcaata tatttattcg
     1741 gctgcctgac ggttgagctt tgttaacgtc tatgttcttg gttttctctt tatactcaat
     1801 aagctttaac gacagcttgg gaattatata tcccgtaaat aaacctaaag ttaagaaaac
     1861 aattaatttg tataacattt catacctctt taatgtattg ttgttagccg cactactgca
     1921 acgaggtcta tgcccttaag ttgaatttat aacaattacc attgctgccg gctaataatc
     1981 ctaaaaatat ccagtacaca tatgccactg ataccacact tatgttaaag aaagctgctg
     2041 tcatataacc tataacactt gataatatta agagtgctgt cttacaattc tttaatctaa
     2101 acaaaccctt taaaattatc tgagaaataa atgttaagta aaccaataaa gatggaatgc
     2161 cgctagtaac tgcaatgtgt aaatattcat tatgtgcctt atcgagatat cgcacctcat
     2221 tccataactc aatcatatct tgggtatagt atttcttaaa tgcttctcct aaattttcta
     2281 taccgtatcc ggtcagagga cgctttttta ttaattctgc aactcgtttc caaatgaatc
     2341 ctctgttggc accggagtaa tcggccctat cacccttagt aagaaactca tcagcatctc
     2401 ttttaataga taaaaatcta tctatgaatg caccttccgt attaaaatta tataaggcta
     2461 agagcaatat tgttataact aacagaataa tatacctggt aaattccccc ttgctatatc
     2521 taaaatacat gtaatgtatt gctgcaaaag ccattattga tgcaattgta cctaaccaag
     2581 ccccccttgt gccggtactt aataagcaat agaacaaaat tgcatatgct gttaaacctg
     2641 ttatgttttt gttgattatg taaaaaaaga ttgttgttgg tattattaaa actatatatg
     2701 aacctaaaaa gttaggatta cccattgttg aaaaagctct tttgctccag ttttcacgga
     2761 ggacatccct tggaaagggg tcaaaattca tagtttgtaa aataccgtaa attgaaacaa
     2821 ttatggcggt aaccagtatt atcttaaaca gcccttcatc gggaaccaag tagagccttg
     2881 ctgttataaa gagaaacata tataaggtta tcgtaaccaa accttcccat ctgccgggca
     2941 ctccgattat tgcaaacact ttattttcag ctgcatatac ggaagcagcc agtaatatga
     3001 agtatataaa taaactaata ttaattctgt ctttctgtac tatatcttta aaacggattt
     3061 tgtttattgc cagcaccgct aaaaagctta atgcaaggat aatcattgct gcgacttttg
     3121 gtaaataaaa atggtctgta gtacctccca atggaacaaa tactgccgga ataactgata
     3181 atgcaagtat taccgtaacc tttgcaatat tatccaattt gctgtttcgt atttcatacg
     3241 aatgtagttt tgttatacca atagtattaa tcat
//
LOCUS       DAOSOW010000213         3250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_29638_length_3250_cov_2.845696, whole genome shotgun sequence.
ACCESSION   DAOSOW010000213 DAOSOW010000000
VERSION     DAOSOW010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29638_length_3250_cov_2.845696"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /gene="rny"
                     /locus_tag="PLD67_06210"
     CDS             <1..1203
                     /gene="rny"
                     /locus_tag="PLD67_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOT22147.1"
                     /translation="KKLKEIEITQEKLDAIHQKQIEELEKISGLSTEEAKSILINNIK
                     KEAEQEAAVAVKEIEKESKETAERKAREIITYAIQKCSADHVAETTVSVVDLPNDEMK
                     GRIIGREGRNIRALEQLTGIDIIIDDTPEAVVISGFDPIRREVARLALEKLIADGRIH
                     PARIEEMVDKAKKEVDQIIKDEGEQATLDTGIHGLHPELVKLLGRLKFRTSYGQNVLK
                     HSIEVSYLAGIMAAELGADVKVAKRAGLLHDIGKAVDHEVEGPHVTIGEDLAKKYKES
                     KAVVHAIAAHHGDTEPQTIEAVLVQAADAISAARPGARRETLETYIKRLEKLEEISNS
                     FEGVEKSFAIQAGREVRIMVKPEVTSDASIVLIAKDIVKKIEEEMEYPGQIKVNVIRE
                     IRATEYAK"
     gene            1319..2923
                     /locus_tag="PLD67_06215"
     CDS             1319..2923
                     /locus_tag="PLD67_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HOT22148.1"
                     /translation="MNFNPAEYKYPSRRKVVYGYNGMVATSQPLAAQAGLEILKNGGN
                     AVDAAIAAAACLTIVEPTSNGLGSDAFALVWFNNKLHGLNGSGYSPYSISIEKLKERG
                     FTEIPKYGIIPIMVPGAPAAWVELSQKFGKLSLIEVLQPAINYAKYGFPVSVTVKKAW
                     DNAYKIYKKENKEEFKYWFDTFTYKGRTPNYGELWKLPYHAETLESIGKTHGNSYYKG
                     AIADKIDSFFKKYDGYLRKEDLEDYKTDWVEPISVNYRGYDVYEIPPNGQGLVALMAL
                     NILKNFDFTYKDKSDTYHKQIEAIKLSFDDGQKYITDIRYMKADIKQLLSDDYGRDKG
                     SLITDNALNLKETDPNSSGTVYLCTADGEGNMVSFIQSNYTGFGSGIVIPETGIALNN
                     RLHNFNYVNQHINCLMPHKKTYHTIIPGFLMKDGKALGPFGVMGAFMQPQGHVQVVSN
                     LIDFHMNPQEAIDAPRWQWIKGMTVEVEQDVPKYIINELINRGHDVKIQLNESSFGRG
                     QMILRTKEGTLCGGTEKRADGHIALW"
     gene            2981..>3250
                     /locus_tag="PLD67_06220"
     CDS             2981..>3250
                     /locus_tag="PLD67_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HOT22149.1"
                     /translation="MSRNFTNEELSKMLYEALKIQKPIDVISDIDNEVTVEDAYDIQL
                     LNVKRALEDGEIVTGKKIGLTSKGMQELLGISEPDYGHLYKSTEVK"
BASE COUNT         1232 a          504 c          724 g          790 t
ORIGIN      
        1 aagaagttaa aagaaattga aataacgcag gaaaaattag atgcaattca ccaaaaacaa
       61 attgaggaac tcgagaaaat atccggactt tcaacagaag aagctaaaag cattctaatc
      121 aacaacataa aaaaagaagc tgaacaggaa gccgcggttg ctgtcaaaga aatcgagaag
      181 gaatcaaaag aaactgcaga aagaaaagca cgtgaaatta ttacatatgc gattcagaaa
      241 tgttctgcag accatgtagc agaaacaact gtatctgtag tcgacctgcc gaatgacgaa
      301 atgaaaggca gaataatagg aagggaaggc agaaatataa gagccttgga acaacttaca
      361 ggtatagata ttataataga tgatacaccg gaagcagtag ttatatccgg atttgaccct
      421 attagaagag aagttgccag attagcactt gaaaagttaa ttgcggatgg taggatacat
      481 cctgcaagaa ttgaagagat ggttgataag gcaaaaaaag aagtagacca aataattaag
      541 gatgaaggcg aacaggcaac ccttgatacc ggaatccacg ggcttcaccc ggaactggta
      601 aaattacttg gcagactgaa attcaggaca agttacggcc aaaacgtatt gaaacactcc
      661 attgaagtat catatttagc aggtattatg gctgctgaat taggcgctga cgtaaaagta
      721 gcaaaacgag caggattatt gcatgatata ggaaaggcag tggatcatga agttgaaggt
      781 ccccacgtta caataggtga agacttagct aaaaagtaca aagaaagcaa ggccgtagtt
      841 catgctatag cagctcatca cggggataca gagccacaga ccatagaagc agtcttagtc
      901 caagcagcag atgctatatc agctgcaaga ccgggtgcaa gaagagaaac attagagact
      961 tatataaaga ggcttgaaaa gttagaagaa atttcaaatt catttgaagg ggtagaaaag
     1021 tcttttgcaa tacaggcggg aagagaagta agaataatgg ttaaacctga agtaacaagc
     1081 gatgcgagca tagttttaat agcaaaggat atagttaaaa agatagaaga agaaatggaa
     1141 tatcccggac agattaaagt taacgtaatt agagaaatca gagctacaga atatgcaaag
     1201 taaaggttag aaaaatatgg aagtaaacaa tttgtttact tctttttaat aacagacaat
     1261 ttctaagcat ccttagggag tgttgtcatg ataaatcaaa gaaaggagta aaaaaaacat
     1321 gaattttaac cctgccgaat ataaatatcc gtcaagaaga aaggttgtct acggatataa
     1381 cggaatggtt gccacatcac agccattagc agcacaagca ggccttgaaa tacttaaaaa
     1441 cggcggcaat gccgttgatg cggctatagc tgcagcagca tgccttacaa ttgttgagcc
     1501 tacatcgaac ggtttgggaa gcgatgcatt tgcccttgta tggtttaata ataagcttca
     1561 tggtttaaat ggcagcgggt attcacccta cagcatttca attgaaaaat tgaaagaaag
     1621 aggatttact gaaattccaa agtacggcat tataccgata atggtacccg gtgctccggc
     1681 agcctgggta gaattatctc aaaaattcgg gaaactttct ttaatagaag tcttacagcc
     1741 tgccattaat tatgccaagt atggatttcc cgtttctgtt actgtaaaaa aagcatggga
     1801 taatgcatat aaaatttaca aaaaagaaaa taaagaagag tttaaatatt ggtttgatac
     1861 attcacctat aaggggagaa caccaaatta cggagagctt tggaagcttc cgtatcatgc
     1921 cgaaacattg gaatcaatag gaaaaacaca cggcaactca tactataaag gtgccatagc
     1981 agataaaata gattcctttt tcaaaaagta tgacgggtat ttaagaaaag aagacttaga
     2041 agattataaa acagattggg tagagcctat aagcgtaaat tacaggggat acgatgtata
     2101 tgaaatacct cccaacggtc agggactggt agctttgatg gcgcttaaca tattaaaaaa
     2161 ttttgatttt acatacaagg ataagagcga tacctatcac aaacaaattg aagctattaa
     2221 acttagcttt gatgatggtc agaagtatat caccgatata agatacatga aagcagacat
     2281 aaagcaatta ttatcggatg attacggcag agataaggga agtttaatta ctgataatgc
     2341 attgaattta aaagaaaccg acccaaacag cagcggaacc gtttatctgt gtacagctga
     2401 tggggaaggg aatatggttt ctttcattca aagcaattat accggatttg ggtcggggat
     2461 tgttatacct gaaacaggaa tagctcttaa caataggtta cataatttta attatgtaaa
     2521 ccaacacatt aattgtttaa tgcctcataa gaaaacctat catacgataa tacccggatt
     2581 cttaatgaag gacggaaaag ctctaggtcc ctttggtgta atgggggcat ttatgcagcc
     2641 tcaaggacat gtgcaggtag taagtaattt aatcgatttt catatgaatc ctcaggaagc
     2701 catagatgca ccgaggtggc agtggattaa aggaatgact gttgaagtag agcaggatgt
     2761 tccaaaatat attataaatg agctgattaa caggggtcat gatgtaaaaa tacagttaaa
     2821 tgaatcatct ttcggacgag gccaaatgat actaaggaca aaagaaggca ctctctgcgg
     2881 cggaacagaa aagagagcag atggacacat agccctttgg tgacaagggg acgggggatt
     2941 agttggacat tagaatatat taatatataa ggaggaaaat atgtctagaa attttacaaa
     3001 cgaagaatta tccaaaatgc tttacgaagc attaaaaatt cagaaaccta tagatgttat
     3061 aagtgatatc gataatgaag taaccgttga ggacgcttat gatattcagc tgttaaatgt
     3121 taagagagca ttggaagacg gggaaattgt aactggcaag aaaatcggcc ttacttcaaa
     3181 gggcatgcag gaactgctgg gtattagcga gccggattac ggccatttat ataaaagcac
     3241 ggaagtaaaa
//
LOCUS       DAOSOW010000214         3221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_29960_length_3221_cov_4.018951, whole genome shotgun sequence.
ACCESSION   DAOSOW010000214 DAOSOW010000000
VERSION     DAOSOW010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29960_length_3221_cov_4.018951"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PLD67_06225"
     CDS             <1..844
                     /locus_tag="PLD67_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOT22150.1"
                     /translation="FDSDEAGQKASLKAINMLKRNNLNAKIVIMKDAKDPDEYLSKYG
                     KPAFDKLIENSLDYYSFLEFHFKEILENSNKVEYINNLFDNIVNVNSEIERELIFDKL
                     SRKVGVSKESIIKEFNKRSTKQKTLDKTAIPAVKPQVAKITTCHEEELIKLILLDNDF
                     ALKLSEIVNEDTFKDLKYYNILKELYECRINDNSIDSKSLNKIIKDDMNVNLQFDNVD
                     YDNLEALFQDCMKRLKIKYYKEKRSILNNILKQSDNSLNRKEIMNEIYCLAKKIKSTK
                     EEVN"
     gene            847..2016
                     /gene="rpoD"
                     /locus_tag="PLD67_06230"
     CDS             847..2016
                     /gene="rpoD"
                     /locus_tag="PLD67_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOT22151.1"
                     /translation="MSENKNISVISEEEYAKNEEMLQKIDSIKNKLIEKGKRNGFITY
                     KEVLRSFEKLDINPEFIDDFYKQAEDNDLEILGFQDDMIPEKVDEEDEIDSKEDADFF
                     VSQSDDDILKGVNIDDPVRMYLKEIGKVSLLSAQEEVELAKRMEAGDEEAKKQLAEAN
                     LRLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGFKFSTYATWWIRQAITR
                     AIADQARTIRIPVHMVETINKLIRIKRQLLQELGRDPTPEEIALEMEMEPEKVREILK
                     IAQEPVSLETPIGEEEDSHLGDFIPDDEVQAPSDVATFTLLREQLSNVLHTLTDREQK
                     VLRLRFGLDDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLKDYLE"
     gene            2278..2952
                     /locus_tag="PLD67_06235"
     CDS             2278..2952
                     /locus_tag="PLD67_06235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT22152.1"
                     /translation="MNRLECIVSMVDKCNTAADIGTDHGYVAEMLLKKGICNKIIATD
                     LNKGPLYRAREHLSEVKLSDKCDFRLGDGLTVLNEGEADTIIVAGMGGDLISEIIERS
                     KHIAESAKQLILQPMTKVDNLRRYLCNNGFEIIDENIVKEYNHFYFVIKTKKGFTKYM
                     DDIYYEISKVLTDKRDTVMIEYINKVIDTNKKIISSLEKADNVAYNEKIEELKRKNKK
                     IRELIR"
     gene            2949..>3221
                     /locus_tag="PLD67_06240"
     CDS             2949..>3221
                     /locus_tag="PLD67_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOT22153.1"
                     /translation="MNLNNIINLLNEEVKLKEHESWDNSGLQLGSMEVNINKIMLTLD
                     INKEAIEYAVQHKVDLIITHHPFFFGSIRKIDFDTYDGEIIKMLIKE"
BASE COUNT         1324 a          400 c          641 g          856 t
ORIGIN      
        1 atttgattcg gatgaagcag ggcagaaagc atctttaaaa gcaattaata tgctaaaaag
       61 aaataactta aatgcaaaaa tagttataat gaaggatgcc aaggacccgg acgaatatct
      121 gagtaaatat ggaaagcccg ctttcgacaa attaattgaa aattcattag attattatag
      181 ctttttagaa tttcatttta aagaaatact tgaaaattct aacaaagttg aatatataaa
      241 caatcttttt gacaatatag taaatgtaaa cagcgaaata gaaagagaat tgatttttga
      301 taaattatca agaaaagtag gtgtttcaaa agagtccata attaaagaat tcaataagag
      361 aagtactaaa caaaagactt tggataagac tgcaataccg gcggtaaaac cgcaggttgc
      421 aaaaattact acatgccacg aagaagagct gattaaactg atactattag acaatgattt
      481 tgcactcaag ctaagtgaga tagtgaatga agataccttt aaggacttaa aatactataa
      541 tatattaaaa gagctttatg aatgcagaat taacgataat agtattgaca gtaagagctt
      601 aaataaaatt ataaaggacg acatgaatgt aaatttgcag tttgacaatg ttgattatga
      661 caatttagag gctttgttcc aagattgtat gaaaagactt aaaataaaat attataagga
      721 aaagagaagc atattgaata acattttaaa acaatctgat aattcattaa accgtaagga
      781 aattatgaat gaaatttatt gcctcgctaa aaagattaaa tcaactaagg aggaggtcaa
      841 ctgacaatga gtgaaaataa aaatatatca gtaatttcag aagaagaata tgcaaaaaac
      901 gaagaaatgt tacaaaaaat agactccata aaaaataaat taattgaaaa aggcaaaaga
      961 aacggtttta taacatataa agaagtattg aggtcatttg aaaagctaga tattaatcct
     1021 gaattcatag atgattttta taagcaagca gaagacaacg accttgaaat attaggtttc
     1081 caagacgata tgatacctga aaaggtcgat gaagaagatg aaatagattc aaaagaagac
     1141 gcggattttt ttgtaagcca atcagatgat gatatcctta aaggtgtaaa tatagatgac
     1201 cctgtaagaa tgtatctgaa agaaatcggg aaggtttctt tattgagtgc acaagaagaa
     1261 gtagagctgg ccaaaagaat ggaagccggt gatgaagaag ctaagaagca gttggctgaa
     1321 gccaatttaa gattagtagt aagcattgca aaaagatatg taggaagagg aatgcttttt
     1381 cttgatttaa ttcaagaagg aaatcttggg cttataaaag cagtggaaaa attcgattat
     1441 acaaaaggtt ttaaattcag tacatatgca acatggtgga tacgtcaggc tattacgagg
     1501 gcaatagcag accaggcaag aacaataaga atacctgtgc acatggttga aacaatcaat
     1561 aagttaatca gaattaaaag acagctattg caggaattgg gaagagaccc tacaccggaa
     1621 gaaatagcat tggaaatgga aatggagcct gaaaaagtta gagaaatact taaaattgcc
     1681 caggagcccg tatctttgga aacacccatc ggtgaagaag aagacagtca ccttggagat
     1741 ttcattcccg atgatgaagt tcaggcacct tcagatgttg caacattcac ccttttaaga
     1801 gagcaactgt caaatgtgtt gcatacttta acagacagag agcaaaaggt actgaggctt
     1861 cgtttcggtt tggatgacgg aagagcaaga acacttgaag aggtcggtaa agagtttgat
     1921 gtaacaagag aaagaatacg tcaaatagaa gctaaggcac tgagaaagct gagacatcca
     1981 agcagaagca aaaaattgaa agattattta gaataaagat gccctaaccc tatttgttca
     2041 atgtatgggg acacacctat actataggga tagtctttgt agttagagga atgtccccaa
     2101 attaggtttt ctgaaaaata gtggtaggtc attcgcaacg agcaccaatt ttgtgcgacg
     2161 aaagtagcaa acaaaattgt gtagggagac tgcgtgtaac actctgaatg ataactatat
     2221 taatatatag atggaatgca acatttacat tccatttatt catttaaggg ggtcagtatg
     2281 aacagattag aatgtattgt atcaatggtc gataaatgca acacagcagc agatataggt
     2341 actgaccatg gatatgtggc agagatgctt ttaaaaaaag gaatatgcaa taaaattata
     2401 gcgactgatt taaacaaagg acctttatac agagcaaggg agcatttatc cgaagtaaaa
     2461 ctcagtgata aatgtgactt taggcttgga gacgggctta ctgttttaaa tgaaggggaa
     2521 gcagatacaa ttattgttgc aggaatgggt ggcgatttaa tttcagaaat aatcgaaaga
     2581 tcaaaacata tagcagagag tgcaaaacag cttattttac agcctatgac taaggttgat
     2641 aatttaagaa gatacctttg taataacggt tttgaaataa tcgatgaaaa tatagtaaag
     2701 gaatacaatc atttttattt tgtaataaag acaaaaaaag gctttaccaa atatatggac
     2761 gatatatatt atgaaataag taaagtatta acagataaaa gagataccgt aatgatagaa
     2821 tacataaata aggtaattga tacaaacaaa aaaataatca gcagtcttga aaaagcagat
     2881 aatgtggcat ataatgaaaa aatagaggaa ttaaaaagaa aaaacaagaa aattagggaa
     2941 ttaataagat gaatttaaat aatattatta atttattaaa tgaagaagta aagttgaagg
     3001 agcatgaatc ctgggacaac agcggattac agctgggctc catggaagtt aacataaata
     3061 aaattatgtt aaccttggac atcaataagg aagcaattga atacgctgtt caacataagg
     3121 ttgatttaat aattactcac catccgtttt ttttcggcag tatcagaaaa attgattttg
     3181 atacatacga cggtgaaatt attaaaatgt taattaaaga a
//
LOCUS       DAOSOW010000215         3209 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_30081_length_3209_cov_2.820862, whole genome shotgun sequence.
ACCESSION   DAOSOW010000215 DAOSOW010000000
VERSION     DAOSOW010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30081_length_3209_cov_2.820862"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(265..1974)
                     /locus_tag="PLD67_06245"
     CDS             complement(265..1974)
                     /locus_tag="PLD67_06245"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stalk domain-containing protein"
                     /protein_id="HOT22154.1"
                     /translation="MKRTVKIIITSFIVLIILTLNVYGLSFEASNHYELENIILEQMR
                     EYNPVFNIKYTGSLDNIEEVLKSMIDKDTYLKSNITRVDWDISGNKTASNINVRVSYI
                     MTKEERIEADKMIDEILADIIKPYMNDHEKVKAVHDYIVLNGKYDNNSLYFSDYDLLT
                     KGTSVCNGYALLTYNMLNKLNIPVNLVSGTSAGEAHIWNMVKLDDYWFHLDVTWNDPV
                     SDRDAVFYTYYMLTEKEICKDHAIDANLKIPKSTKEYYDYLVELSYNKLLVETGLDMY
                     NEENFAADESELKNLLTRKITHHPLMITVRFDKSISQDSIINAMSQLYKYDYISVINY
                     SLIDNDSKGEWNILNIFIKYKETPDNITLDFARSVYNTATEVDYNVYAQYGNKKINIT
                     KDVYIYPYDTNKINVSKGTLKFKEPGNYNLTFEYQGLRETVSITGLNSNAFEYITDKK
                     PDNYVNVKVYDQYIDFSSINQWPIIENDRTMVPLRAVFEVLNCNVKWEESSKSAVVEH
                     GSTKIIIPANSTTAYINGKANSLDVPAKIVNDRIMIPLRFVSEAIEKTVIWDDPNKTV
                     LIY"
     gene            2167..2742
                     /locus_tag="PLD67_06250"
     CDS             2167..2742
                     /locus_tag="PLD67_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOT22155.1"
                     /translation="MYKRILNEILLSEIPSEGIYKLMDSGEMNNILPELLRLKGFEQQ
                     TPYHDKDVLEHTLAVVDEIKPKLNLRLAALLHDISKPDCFTVDENGRGHFHGHHVKSA
                     LASEKILQRLGYEEELILNVTILIRYHYIKDIAKVIKEKGIKKFVENVGAERLDDIFE
                     LIRADMTGKASTNYEVIEKLRDMCNKYEEKQ"
     gene            complement(2900..>3209)
                     /locus_tag="PLD67_06255"
     CDS             complement(2900..>3209)
                     /locus_tag="PLD67_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22156.1"
                     /translation="GNLTYIVYIDEASGEMVKLEMDIAEMIASMVSGMAEITGMPPEA
                     IEVLNSLKAYMTVEFLNINSAEDFEIPEEALNAPDMLEGLQQQLETEIEIEFEAEEEL
                     "
BASE COUNT         1074 a          522 c          497 g         1116 t
ORIGIN      
        1 taaataatgt catcctgagc gtaagcgaag gatctcaagg tttaaaaaat gagattcttc
       61 gagctaaagc tctcagaatg acggtagctt attcatagca ataacaaggt tgcgaattta
      121 tgacatatta acatagtaga agagacagtt atctgtctct tctgttttta aaatattgaa
      181 ttattaaggc acctattatt aaaagaaaca taatcggata aataatatac ataaatccga
      241 aatcaatagg catgttgcta cctcctaata aatcagcact gttttgtttg gatcatccca
      301 aatgactgtt ttttcaatag cctcgctgac aaatctaagg ggtatcatta tgcggtcatt
      361 tactattttt gcaggaacgt ccaaagaatt agctttgccg ttaatataag ccgtggtaga
      421 gtttgcaggt ataattattt ttgttgagcc gtgctcaaca actgcagact tagaagattc
      481 ttcccacttg acattacagt ttaatacttc aaagacggct cttaatggaa ccatagtcct
      541 gtcattttct ataataggcc actgattaat tgaactgaaa tctatatatt ggtcataaac
      601 tttaacatta acgtaattgt ccggtttttt atcagtaatg tattcaaatg catttgaatt
      661 taatcccgtt atggatactg tttcacgaag tccctggtat tcaaacgtta agttgtagtt
      721 tccgggctct ttgaatttta aagttccttt tgaaacattt attttatttg tatcataggg
      781 ataaatatat acatcctttg taatgtttat ttttttgttg ccatattggg catagacgtt
      841 atagtctacc tccgttgcag tattgtaaac actccttgca aagtctaaag ttatattgtc
      901 aggtgtttct ttatacttaa taaatatatt taaaatattc cattcgcctt tactgtcatt
      961 gtcaatcaga ctgtaattaa ttacggatat ataatcgtat ttatataatt gagacatagc
     1021 attaattata ctgtcttgag aaattgactt gtcaaatctt acggtaatca ttagaggatg
     1081 atgtgtaatt ttacgagtca gtaaattttt taactcagat tcgtccgcag caaaattctc
     1141 ctcattatac atatcaagcc ctgtttcaac caacaatttg ttataggata attctacaag
     1201 gtagtcataa tactccttgg ttgatttagg tattttcaaa tttgcatcaa ttgcgtgatc
     1261 tttacaaatt tctttttccg ttagcatgta gtatgtgtaa aaaactgcat ctctgtctga
     1321 caccgggtca ttccatgtta catctaagtg aaaccagtag tcatctaatt tgaccatatt
     1381 ccaaatatga gcttctcccg cggaagtacc ggataccaaa ttaaccggaa tattaagctt
     1441 gttaagcata ttgtaggtta aaagagcata tccgttgcaa actgaagttc ctttcgtcag
     1501 caaatcatag tccgaaaaat ataaagagtt gttatcatat ttaccgttta atactatgta
     1561 atcatgaact gctttaacct tttcgtggtc attcatgtaa ggtttaataa tatcagccaa
     1621 gatttcgtca atcattttat ctgcttcaat tctttcttcc ttagtcataa tataagaaac
     1681 tcttacattt atgttgcttg cagtcttgtt gcctgatata tcccaatcca cccgcgttat
     1741 gttagatttt aaataagtat ccttatctat catacttttt aatacttctt caatattgtc
     1801 caaggagccg gtatatttaa tattaaaaac cggattatac tccctcattt gctcaagaat
     1861 aatattctca agctcataat gattgcttgc ttcaaaagac aaaccataaa cattaagagt
     1921 aagaataatt aagactataa aagaggttat aattatttta acagttcgtt tcattaaatc
     1981 accttcctga ctcttctggt atgattatat accattttac ataaaataaa tagtattata
     2041 ttttacaatt acaatcgaat gtaatataat tgtttccgta cacactaaac aacaacaggt
     2101 gagcgtttta tgccgtatta ttaattagac gtaaaatttt agaaaaagtt tcataaaagg
     2161 agaaatatgt ataaaagaat tttaaatgaa atattgttaa gtgaaatacc ctcagaagga
     2221 atatataaat taatggatag cggtgaaatg aacaatatac ttcctgaact tttaaggctt
     2281 aaaggctttg agcaacaaac accttatcat gataaggatg tattagagca tacattggct
     2341 gtagtagatg aaattaaacc gaaacttaat ttaagattgg cagctctttt gcacgatatt
     2401 agtaagcctg attgttttac ggttgatgaa aacggaagag gtcattttca cgggcatcat
     2461 gtaaagtctg ctcttgcaag tgaaaaaatt cttcaaagac ttggatatga agaggaatta
     2521 attctgaatg taacaatttt aataagatac cattatataa aggatatagc caaagttata
     2581 aaggaaaaag gaataaaaaa atttgtagaa aatgttgggg cagaaagact tgacgatata
     2641 tttgaactta tcagagccga tatgacaggc aaagcttcaa caaattatga agtaattgaa
     2701 aaattaagag atatgtgcaa taagtatgaa gaaaaacaat aggaatgatt tgtagataca
     2761 tcagcaacag ggcttaaatt taaatttata cccaaggatt tcaaaagctt tgatttgcta
     2821 ttaattatac taacatttca taaaatataa tcaataaaaa aagtgcaccg aaaagtgcac
     2881 ttttcatcgt ttctactcat tacagttctt cttcagcttc aaattctatc tcaatttcgg
     2941 tttctaattg ttgttgtaac ccttcaagca tatccggtgc gtttaaagct tcttccggaa
     3001 tttcaaaatc ttcggcgctg ttgatattta aaaattccac tgtcatataa gcctttaagc
     3061 tgtttaaaac ttctattgct tcaggaggca taccggttat ttctgccata ccggatacca
     3121 tagatgcaat catttctgct atatccattt ctaattttac catttcaccg cttgcttcat
     3181 caatatatac gatgtatgtt aaatttcca
//
LOCUS       DAOSOW010000216         3177 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_30450_length_3177_cov_3.773222, whole genome shotgun sequence.
ACCESSION   DAOSOW010000216 DAOSOW010000000
VERSION     DAOSOW010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30450_length_3177_cov_3.773222"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PLD67_06260"
     CDS             complement(<1..1080)
                     /locus_tag="PLD67_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOT22157.1"
                     /translation="MKDSKIDTTLINRLIDEEIIRYWNTEPEKANINQIYRAFSIVIR
                     DLLLKKRNKFKRMQNEHKQVYYICMEFLIGKSLRNNLFNLGIEDEAKEALKKYNITPE
                     ELYSIERDPGLGNGGLGRLAACFMDSLATQDYAAVGFSIKYEFGLFKQKLIDGWQTEL
                     PDDWLTTSDVWLMPRTDERVIVKFDGTIREEWTGEGLKIHHDCPTEIEAIPYDMMISG
                     YNSKGVSLLRLWESRSINSIDMNLFSQGDYIRALSEDSMAEAISKVLYPADDHNEGKI
                     LRLKQQYFLVSASAQNIVKNHMKKYNSLDNFSEKNAIHINDTHPSLIIPELMRIFMDE
                     YGLSWEKAWTIVVNTVSYTNHTVLKE"
     gene            complement(1067..2524)
                     /locus_tag="PLD67_06265"
     CDS             complement(1067..2524)
                     /locus_tag="PLD67_06265"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOT22158.1"
                     /translation="MNKLKVLYVASEAAPFIATGGLGEVAGSLPKALVEKYKDKIDIR
                     IVLPLYQGIVDRSEFEFMGKTSVPLAWRQQYCGVYKTILNNVTYYFIDNEYYFKRSEC
                     YGHFDDAERFAFFSKAVLYILPMIKFMPDIIHANDWQTSLIPIYLSAKYSGYEGYRDI
                     KSIITIHNIEYQGVFDLAISEDVFDLHGKEKGIIEFKGAINLLKGALETAHFISTVSE
                     SYSKEILDDYYAHGLAEIIQKNSSKIKGILNGIDTEKYNPEDDTEIFENYNAESIQKK
                     YLNKINLQSLSGLPQDEDIPVIGIISRLVKHKGFDLIKESMEEILKEKVQFIILGKGD
                     RGYELFFKYLQESYRDKIYVRIGFDSDFAKKIYSGADLFLMPSISEPCGIAQMISSRY
                     GTVPIVRETGGLKDSIKDASLGKGNGFTFVDETPQALCEAVKRALIVYENKEDWRKLV
                     ETVMKMDFSWRKSAEKYFDLYSSLANFEENNNEGF"
     gene            complement(2535..>3177)
                     /locus_tag="PLD67_06270"
     CDS             complement(2535..>3177)
                     /locus_tag="PLD67_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HOT22159.1"
                     /translation="SIHKILYEFDEDGRADDILVYPVTSGNYNIGLNTWVMNKEFLES
                     LIYDAIARNYKDFSFDVLSARVHSLRIYGYEYTGFFAHIDSLQSYYENSMKLLNMDTL
                     QNIFYSNTGPIYTKTKDTAPAKYCKDAVVKNSLIANGCIIEGTVENSILFRDVRVGKN
                     AVVKNSIIMQHGRISDNAKLDYIITDTYTFVNKDRVLLGYSNCPFFIKKNSVV"
BASE COUNT          935 a          640 c          459 g         1143 t
ORIGIN      
        1 ttccttcaat accgtgtgat ttgtgtagga taccgtatta actacaatcg tccatgcttt
       61 ttcccacgat aatccgtatt catccataaa aatacgcatt agctcaggaa ttatcaatga
      121 agggtgggta tcatttatat gaattgcatt tttttcactg aagttatcca atgaattgta
      181 ttttttcata tgatttttaa caatattttg tgctgatgca gaaaccaaaa agtattgctg
      241 cttaagtctt aatattttac cctcattatg gtcatcggca ggatataata cttttgatat
      301 tgcttctgcc attgagtctt cagataaggc ccttatatag tctccctgtg aaaacaaatt
      361 catatcaata ctgtttatac ttcgggattc ccaaagcctt aaaaggctga cacctttgct
      421 gttataaccg gaaatcatca tatcataagg tattgcttct atttccgtag ggcaatcatg
      481 atgtatctta agtccttcgc cggtccattc ttctcttatt gtgccgtcaa actttacaat
      541 gactctttcg tctgtcctgg gcataagcca tacatctgaa gtagtcagcc agtcatcggg
      601 aagctccgtc tgccatccgt caattaactt ttgtttaaaa agtccgaatt catacttaat
      661 tgaaaaccca actgccgcat agtcttgtgt tgcaagagag tccatgaagc aggcagcaag
      721 ccttccaagt cctccgttac ctaagcccgg gtctctttcg attgaatata gttcttcggg
      781 agtaatatta tacttcttca gagcttcttt cgcctcatct tcaatgccta aattaaacag
      841 gttattgcgt agagattttc caataagaaa ttccatgcat atatagtata cttgtttatg
      901 ttcattctgc attctcttga atttatttct tttttttaag agcaaatccc ttattacaat
      961 tgaaaatgcc ctgtaaattt gatttatatt cgccttctca ggctcagtat tccaatatcg
     1021 aataatttct tcatctatta aacggttaat cagtgttgta tcgattttag aatccttcat
     1081 tattattctc ctcaaaattt gccagactgc tatacaagtc aaaatatttt tctgcagatt
     1141 ttctccagct gaaatccatt ttcataactg tttcaactaa ttttctccaa tcctctttat
     1201 tttcataaac tattagagcc cttttgacag cttcgcacaa tgcttgaggg gtttcatcaa
     1261 cgaatgtaaa accattgcct tttccaaggc ttgcatcctt tatcgaatcc ttaagtcctc
     1321 cggtctctct aacaatcgga actgttccgt aacgggaaga aatcatttga gcaataccgc
     1381 aaggctcact tattgaaggc atcagaaata aatctgcacc tgaataaatc ttttttgcaa
     1441 aatcagaatc aaagccgatt cttacgtata ttttatcacg ataactttct tgaaggtatt
     1501 taaagaataa ttcatatccc ctgtctccct ttccgagtat gataaactga accttttcct
     1561 ttagtatttc ttccatggat tcttttatca ggtcaaatcc cttatgctta accagtcttg
     1621 atataatccc tattacggga atgtcttcat cctgcggcag ccctgataat gattgtaaat
     1681 ttattttgtt taaatatttc ttttgaatac tttctgcatt gtaattttca aatatttctg
     1741 tatcgtcttc aggattgtat ttttcggtat ctattccgtt tagtattccc ttaatcttgg
     1801 atgaattttt ttgtataatt tctgccagcc cgtgagcata ataatcatct aatatttcct
     1861 ttgaatagct ttcgcttacc gtactaataa aatgagcggt ttccaaggca cctttcaaaa
     1921 ggtttattgc tcctttaaat tcaattatac ctttttcctt tccgtgcaag tcaaatacat
     1981 cctctgaaat agctaaatca aacacccctt gatattcaat gttatgtatt gttattattg
     2041 attttatatc tctgtaaccc tcataaccgg aatattttgc agataaataa atgggaatca
     2101 atgatgtctg ccagtcattt gcatgaataa tgtcaggcat aaattttatc ataggcagta
     2161 tatacaatac cgccttagag aaaaatgcaa atctttcggc atcatcaaaa tgaccgtagc
     2221 attcactcct tttaaaatag tattcattgt ctataaaata atatgtaaca ttatttaata
     2281 tggttttata aacgccgcaa tattgctgtc tccaggcaag aggaacagat gttttcccca
     2341 taaattcaaa ttcacttctg tccacaattc cttggtacaa aggaagtact atccttatat
     2401 ctattttgtc cttatatttc tcgaccaatg ctttcggcaa tgacccggca acttcaccaa
     2461 gtccgccggt tgctataaag ggtgcagctt cggatgcaac atataatact tttaatttat
     2521 tcatataatc actcctatac aactgagttt ttctttataa agaagggaca gttagaatat
     2581 cccaacaaaa ccctatcctt gttcacaaaa gtgtatgtgt cggttattat gtagtccaat
     2641 ttggcattgt cgcttattct tccatgctgc ataataattg aatttttaac aactgcattc
     2701 ttgcctactc tcacatctct gaaaagtatg gaattttcga cagttccttc aatgatacag
     2761 ccgttggcaa tcaaagaatt tttaacaact gcatctttac aatacttagc aggagccgta
     2821 tcttttgttt ttgtatatat gggacctgta tttgaataaa agatattttg caatgtatcc
     2881 atatttaaaa gtttcatact gttttcataa tagctttgca aagagtcaat atgtgcaaaa
     2941 aatccggtat attcatatcc gtaaattctt aaagaatgaa ccctggcaga taaaacatca
     3001 aagctaaaat ccttgtaatt tcttgcaata gcatcataaa taagggattc caagaattcc
     3061 ttgttcataa cccatgtatt taatcctata ttgtaatttc cgcttgtaac cggataaact
     3121 aagatatcat cagctcttcc atcctcatca aattcatata atattttatg aattgaa
//
LOCUS       DAOSOW010000217         3175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_30486_length_3175_cov_3.428526, whole genome shotgun sequence.
ACCESSION   DAOSOW010000217 DAOSOW010000000
VERSION     DAOSOW010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30486_length_3175_cov_3.428526"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /locus_tag="PLD67_06275"
     CDS             complement(<1..1727)
                     /locus_tag="PLD67_06275"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOT22160.1"
                     /translation="MKKIKGLELSSQQINAASFDKGNALVLSTAGSGKTTVIISRAGR
                     LLFENKCSKKILTITFSKMAANDMKKRFACIGNCQNYETKTEFSTIHAFSYKIVRDFY
                     RRKGINFGLLTNNYQALSEILKNHYSKSYFSYVSDEEIENLSSAIGYVNNMMIAPEEY
                     KDYGIEIKGFDKLYNDYKMYKSQNRLIDFDDMLDYSYKILSDYANYKEMILNTFEYVQ
                     IDEMQDTSKIQHELIKLITRDNLFMVGDDDQAIYSFRGSYPDFMLKFKDIFNDGKIFY
                     LDNNYRSDKYIVEGARNFIEINKNRYKKAIKTENSKENEINIIKVKDRAEQAKLITDI
                     VAKAKYENKTIGILYRYNMSAMIPANNLYENNIDFYIKEDKTKFFSSAVLYDILAFLN
                     LAINPKDKDSYARIYYKSYTYFTKGMCKMVLESNEDKSVLDILYGYKYFDYNVYDKIK
                     QFKTDIDYLNKLKPSNAIRFIKFDLEYLNYLERMQEEGRYSLSTSLHILEILEEIGSY
                     CSDIKEFLRKINNLRDVITRASENGNALVTMTTIHSAKGLEYDYVYMIDNLNELNIDK
                     NLSVEDYRS"
     gene            2203..3072
                     /locus_tag="PLD67_06280"
     CDS             2203..3072
                     /locus_tag="PLD67_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22161.1"
                     /translation="MKEKKYKSNQKKALIAFITIIIIIAAIATVEKLIEPMGYFRPDY
                     DKRDITGILEKEVLSDDDYKELFYQTGLGKTAVDEILKDKSGKEKILEFQKNFFTEYN
                     ISSEKFAVIVNHESLVDEKGKIIYGFDLAPYENGYVLITKATHSLGWRHGHAGIITDA
                     ENEETLEAVLLGQDSMLQNINKWRSFPSFIMLKLKDASQEELDKIAEFAKINLLDVPY
                     GLSVGLTSKKNPEPSNITTTQCAHIVWYPFMQFGYDIDSDGTWLVTPKDIANSDLFEV
                     VQIYGVDPDEIWP"
BASE COUNT         1027 a          577 c          490 g         1081 t
ORIGIN      
        1 ttgcttcgat agtcttcaac ggataagttt ttatcaatat ttaattcatt taaattatca
       61 atcatataaa cataatcata ttccaatccc ttggcactgt ggattgtagt cattgttaca
      121 agagcattgc cgttctctga agctctagtt attacatctc tcaaattatt gatttttctt
      181 aaaaattcct ttatatcgct gcagtatgag cctatttcct ccaaaatctc caatatgtga
      241 agagaggttg ataagctgta tcgtccttcc tcctgcattc tctccaaata atttaaatat
      301 tccaaatcaa attttataaa tcttatcgca ttggatggct tcaacttgtt taaataatca
      361 atatccgttt taaactgctt aattttgtca taaacgttat agtcaaaata cttgtacccg
      421 tacaaaatat caagaacact cttgtcttca ttgctttcca gtaccatctt acacataccc
      481 ttggtaaaat aggtgtagct tttgtaatat atccttgcat atgaatcctt gtccttcggg
      541 tttattgcca aattcaaaaa tgcaagaata tcataaagta cagcactgct gaaaaactta
      601 gttttatcct ctttaatata aaaatcaata ttattttcat agagattgtt tgccggtatc
      661 atagccgaca tattgtatct gtataaaata cctatggtct tattttcata ctttgctttt
      721 gcaactatat ccgtaatcaa cttagcctgc tctgctctgt ctttaacctt gattatatta
      781 atttcatttt cttttgaatt ttctgttttt atagcttttt tatatctgtt cttatttatt
      841 tcaataaaat tccttgcacc ttccacaata tatttgtctg agcggtaatt gttgtccaag
      901 taaaatattt taccgtcatt aaaaatatct ttaaacttaa gcatgaaatc cggatagctt
      961 cccctgaatg aatatatggc ttggtcatca tctcctacca taaataaatt gtcacgtgtt
     1021 ataagcttaa tcagctcgtg ctgaatttta gaagtatcct gcatttcatc aatctgcaca
     1081 tattcaaatg tatttaaaat catctctttg taatttgcat agtctgataa tattttatat
     1141 gaatagtcca gcatgtcgtc aaaatcaatc aatctgtttt gtgacttgta cattttgtaa
     1201 tcattgtaaa gcttatcaaa accttttatt tctattccat aatctttgta ttcttcggga
     1261 gcaatcatca tattgttcac atatccgatt gcagaagata aattttcaat ttcttcatca
     1321 ctcacatagc tgaaatatga tttggaataa tgatttttaa gtatttccga aagggcctga
     1381 taattattcg taagcaatcc aaaattaata cccttcctcc tgtaaaaatc cctcacaatt
     1441 ttgtaagaaa aggcatgaat agttgaaaat tcagtttttg tttcataatt ttgacaattt
     1501 ccaatacatg caaatctttt cttcatgtca ttggctgcca ttttagaaaa tgtaatggtt
     1561 agaatttttt tgctgcattt attctcaaat aaaagccttc ctgcccgtga aatgataacg
     1621 gtagtcttgc ctgagcctgc agtggaaaga accagtgcat ttcctttatc aaaagaggcg
     1681 gcatttatct gttggctgct taattctaac cccttaattt ttttcaaata atcaaagtac
     1741 tccatatatc accacaatta gagacgtttg aggctcgttt gctttgctca gagtgacaat
     1801 tcttcgcaac ctgacgggta cggtttttaa acgtcccata tataagaatt atactattta
     1861 aaaaaagtat gccaataaac tcataaatgc ggttaaaacc attcctacgg ccaagattaa
     1921 acaaataact ctcttcattc tatcagcttt tttatatttg ttcatgtaca ttcctccaac
     1981 ttacttatat acatattgta acataaaaaa tgcccctttg ccattgtaat ttttacttat
     2041 tatttatata atatagtaga atccgctttg tcatcctgag cggaagcgaa ggatctcggt
     2101 tttgtcatcc tgagcggaag cgaaggatct catgagattc ttcgggctag ccctcagaat
     2161 gactctccac gtaagacact ataagaaaca atggtagaaa ctatgaaaga aaaaaagtat
     2221 aaatcaaatc aaaagaaagc acttattgct tttataacaa taattattat aattgctgca
     2281 atagcaacag ttgaaaagct cattgagcca atgggctatt ttcgtcctga ttacgataaa
     2341 agagatatta ccggtatatt ggaaaaggaa gtattaagtg acgatgacta caaagagctg
     2401 ttctatcaaa caggcctcgg taaaacagcc gttgatgaaa ttttgaaaga taagtccggc
     2461 aaggaaaaaa tactcgagtt tcagaaaaat tttttcacgg aatacaatat atcaagcgaa
     2521 aaatttgcag taatagtaaa ccatgaatct cttgttgatg aaaaaggaaa aataatatac
     2581 ggatttgacc ttgctcccta tgaaaacgga tatgtattga taacaaaagc aacccattca
     2641 ttaggctgga ggcatggtca tgcaggaatc ataacagatg cagaaaacga agaaactttg
     2701 gaagcggtac tattaggtca ggattcaatg cttcaaaata ttaataagtg gagaagtttc
     2761 ccttctttta taatgcttaa attaaaagac gcatctcagg aagaattaga taaaatagct
     2821 gaatttgcca aaataaattt attagacgtt ccctacgggc tttctgtagg actgacaagc
     2881 aaaaaaaatc ctgaaccgag caatataaca actacccagt gtgctcacat tgtttggtat
     2941 ccatttatgc agttcggata cgatattgat tcggacggta cttggcttgt gacaccaaaa
     3001 gatattgcaa acagtgattt gtttgaagta gtccaaatat acggagtcga ccccgatgaa
     3061 atctggccat aataaatgag atccttcgct ccgctcagga tgacaacttg ttatttctgt
     3121 ggacaacccg tagggtgtca ttctgagggc tttagcccga agaatctcat gagat
//
LOCUS       DAOSOW010000218         3153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_30787_length_3153_cov_3.528405, whole genome shotgun sequence.
ACCESSION   DAOSOW010000218 DAOSOW010000000
VERSION     DAOSOW010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30787_length_3153_cov_3.528405"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..1241
                     /locus_tag="PLD67_06285"
     CDS             228..1241
                     /locus_tag="PLD67_06285"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005977447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOT22162.1"
                     /translation="MKKYNVAILGATGAVGQEMLKVLGERNFPINDLKLLASKRSAGA
                     IVEFNGEEYAIEEATESSFENIDVVLCAAENDISEKLSPAAVKAGAVVVDNSSAFRLY
                     DDVPLVVPEVNGEDVKNHQGIIANPNCSTTIALTAINELNKYSPIKRMIVTTFQAVSG
                     AGVNGIKELNQQIKDISEGKDVEINTFQHQIAYNVIPQIGGFNEMGYSQEEMKLLNEG
                     RKILHNPDLKVNCTCVRVPVYRSHSESITIETEKEITVEKARELLSKAAGVKLIDDLE
                     NNKYPMPLDSSDQDLIYVGRIRKDISGENSLVLWCCGDQIRKGAATNAVQIAEVLVKE
                     NII"
     gene            complement(1303..3045)
                     /locus_tag="PLD67_06290"
     CDS             complement(1303..3045)
                     /locus_tag="PLD67_06290"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOT22163.1"
                     /translation="MKKAISLVLVIIIFFSAAQPCVLAADIETYNQLDKMVQRIVDEG
                     KSKGAVLSVVKDGNIELCKGYGLADDNLGIASDGEKIAFRIGSVSKTFVAVAAQILVE
                     KELLNMDSDISIYLDLDLKLTYPVSMRQLLTHTAGFEDMVTGMAFYNVSDTEPLEISV
                     RKYLPRQIYKPGEVISYSNYGIALAAYVIECITEQDFALFCEEKIFEPLNMNRTTFRF
                     MHDKAYVSKAYSPNGNETIEPYMNLYPEGSAVSTAEDMAKYMIWLMDGDDNRVLSPDS
                     KAELFKKQFTMSEEFEGIGLIWNRKEQDNKVYYDKKGETLNFYTRIALYSEADTGVFL
                     SFNTYVPEHEINNIMNKATDLIYGEKQEYTIKNADSSIKIGGLYVNNWSSFNTPEKIL
                     RYFIPNKMIYIDTEKDGNYTNGQKMTLIGEDLYSSNLGLLKFKEVQNKIIMSSESAIT
                     YVKVPLWQHRIIQIFIPIIFVIVTFIIFIRELIKKFSNKNNKYSFAFALNSFFQIVSF
                     VILCLLMYKGIVTFSILKYAPYMKFLGWFIASLVISNIIIAIKNKKYKELLPAIWVLS
                     GILFSAWMLFFHIL"
BASE COUNT         1097 a          527 c          543 g          986 t
ORIGIN      
        1 gaagttttga gcatgttttt tatttacaat catcataata taattggaat ttggaacatt
       61 ttaaagccta tgcaatcaat ccgtaaaaag atgtacactg aattaaaaca catgtacata
      121 aaataaagta caaaaaataa atcaaaaagc ttgacaagct aaaatttatt gttataatat
      181 tctttaaata tttaaaagca ggtaagcttg cagaaggtga ggacgatatg aaaaaataca
      241 atgtagcaat attgggagca acaggcgcag tagggcaaga aatgctaaaa gtgttgggag
      301 aaaggaattt tcccataaat gatttaaaat tgctggcaag caaaagaagt gcaggagcaa
      361 tagttgaatt caacggggaa gaatatgcaa tcgaagaagc aacagagtca tcctttgaaa
      421 atatagatgt tgtgttgtgt gccgctgaaa atgatataag cgaaaaattg tctcctgctg
      481 ctgtaaaagc aggtgcggtt gtagttgata attcaagtgc attcagatta tatgatgatg
      541 ttcccctggt ggtacctgaa gttaacggag aagacgttaa aaaccatcag ggaataattg
      601 caaacccaaa ctgctcaaca acaatagctc taacggcaat aaatgagctt aataagtatt
      661 ctccaataaa aaggatgata gttacaacat ttcaggcagt atcgggtgcc ggcgttaacg
      721 gtataaagga acttaatcag cagattaagg atataagtga aggaaaagat gtagaaataa
      781 atacattcca gcatcagata gcatacaatg taattcccca aataggaggc tttaatgaaa
      841 tggggtattc gcaggaagaa atgaaattgc tgaatgaagg aagaaaaatt cttcataatc
      901 ctgacttaaa agttaattgt acatgtgtaa gggttcccgt ttacagaagc cattccgaat
      961 caattaccat agaaacagaa aaggaaataa cggtagaaaa agcaagagag ctgttgtcaa
     1021 aagcagcagg cgtgaagtta atcgatgatt tggaaaacaa taaatatcca atgccccttg
     1081 attcatcaga ccaggattta atttatgtag gcagaatcag aaaggatata agcggcgaaa
     1141 attcattggt tctgtggtgc tgcggagacc aaataagaaa aggtgcagct acaaatgcgg
     1201 tacaaatagc agaagttttg gtaaaagaaa atataattta gttttcccgg tgccattttc
     1261 ccggtgccag gcaccattgt ggtgcctggc accgatgtct atttatagga tatgaaagaa
     1321 tagcatccat gctgagaata atattcctga tagaacccat attgcaggca gcagttcctt
     1381 atatttttta ttttttattg caataattat atttgatata acgagtgatg caatgaacca
     1441 tccgagaaat ttcatgtaag gagcatattt aagaattgaa aaggttacaa ttcctttata
     1501 cataagcaaa cataagatga caaacgaaac aatttggaaa aaggaattca atgcaaatgc
     1561 aaaagaatat ttattgtttt tattactaaa ctttttaatc agttctctaa taaagatgat
     1621 aaatgttacg ataacaaaaa ttatcggtat aaaaatttgg ataattctat gctgccacaa
     1681 tggaactttt acatatgtta tggcagattc tgaagacata attattttat tttgtacttc
     1741 cttaaatttt agcaatccta aatttgacga atacaaatcc tcaccgatta aagtcatctt
     1801 ttgaccgtta gtataatttc cgtctttttc cgtatctata taaatcattt tgttaggtat
     1861 aaagtaacgc agaatttttt cgggagtatt gaagctggac caattattta catataaacc
     1921 gcctatctta attgatgagt cggcattttt tatagtgtat tcttgtttct cgccataaat
     1981 caagtcagtg gctttattca taatattatt aatttcatgc tcaggtacat aagtgttaaa
     2041 tgatagaaaa actcccgtat cagcttccga ataaagggct atccttgtat aaaaatttaa
     2101 ggtttctcct tttttatcat aataaacttt attgtcttgt tctttcctgt tccagattag
     2161 tcctatacct tcaaattcct ctgacattgt aaattgtttt ttaaacagct ctgctttgga
     2221 atcaggtgat aatacacgat tatcatcacc gtccatcaac caaatcatat attttgccat
     2281 atcctctgcc gttgacacag ccgagccctc cggatataag ttcatgtaag gctcaatagt
     2341 ttcattgcca ttcggcgaat atgcttttga aacataggct ttatcatgca taaatctaaa
     2401 tgttgtccta ttcatattta aaggctcaaa aatcttctcc tcgcaaaata aggcaaaatc
     2461 ctgctctgta atacattcaa ttacatatgc ggcaagtgct ataccgtaat tcgaataaga
     2521 tataacctca cccggcttat atatttgtct tggcaggtac ttacgtacac ttatttcaag
     2581 aggttcagta tcgcttacat tataaaaagc catacctgta accatatctt caaatcctgc
     2641 ggtatgtgtg agaagctgtc tcatagaaac aggataagta agttttaaat ctaaatccag
     2701 ataaattgat atatcactat ccatgttgag caattctttt tcaacaagaa tttgagctgc
     2761 aaccgcaaca aaggtttttg atactgagcc gattcgaaat gcaatttttt ctccatcaga
     2821 agcaatgccg aggttatcat cagccaagcc ataccctttg cacaattcga tatttccatc
     2881 ctttacaaca gacagtacgg cacctttgga ttttccttca tccactatcc tttgaaccat
     2941 cttatccaac tgattgtatg tttctatatc ggctgccaaa acgcacggtt gtgcagctga
     3001 gaaaaaaatt attataacta atactaaact tattgctttt ttcataatat cctcctttga
     3061 ttttttaaat ttattttatt ttgacagcct gaaatataaa tgttatttac cttaaatttg
     3121 ccttaaatta acaagaaagt atttattcaa cat
//
LOCUS       DAOSOW010000219         3147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_30861_length_3147_cov_3.004204, whole genome shotgun sequence.
ACCESSION   DAOSOW010000219 DAOSOW010000000
VERSION     DAOSOW010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30861_length_3147_cov_3.004204"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLD67_06295"
     CDS             complement(<1..313)
                     /locus_tag="PLD67_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HOT22164.1"
                     /translation="MTDKLTYKDSGVDVNSGYETVKLFKEHVRKTHIKGVISNIGGFG
                     GMFEIDKDEYEEPVLVSGTDGVGTKLMIAFMADKHDTIGEDAVAMCVNDVICQGAKPL
                     FF"
     gene            complement(323..1711)
                     /gene="purF"
                     /locus_tag="PLD67_06300"
     CDS             complement(323..1711)
                     /gene="purF"
                     /locus_tag="PLD67_06300"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOT22165.1"
                     /translation="MFNLEEDKLHEECGVVGVYSDNPDITSQLIYYGLFALQHRGQES
                     AGIAIHTGKRIEFHKDMGLVADVLSQKVIDSLKGNIAIGHDRYSTSGESEKQNAQPLV
                     VRYKEGSIALAHNGNLVNADSIREILENEGVIFQTSTDSEVVANLISRHYRGSIIKSI
                     QTTMEVVKGAYAFVVMTDKELIGVRDNYGLRPLCLGKREDGYLLASESCGITAMGGEF
                     IRDIEPGEIVVINEEGVNSIKTTKYAQQRLCIFEHVYFARPDSNLDGVNVYETRYKSG
                     RLLAQEAPVKADLVFCVPDSGTPAALGYAEELSIPYGLGIIKNRYAKRTFIEPTDKLR
                     QQGVKTKLSILKEIVQDKVVVMVDDSIVRGTTTKQIVNMVREAGAKEVHVRIASPPVT
                     HSCFFGINTPDRKELLGANKTIEEIKDYIKADSIHFLSLENLIKSTGSNLGYCKACLN
                     GDYPMEVPNNGI"
     gene            complement(1768..2262)
                     /gene="purE"
                     /locus_tag="PLD67_06305"
     CDS             complement(1768..2262)
                     /gene="purE"
                     /locus_tag="PLD67_06305"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOT22166.1"
                     /translation="MKVAIVMGSDSDFPVVEKGYKVLKDFEVMADVRVISAHRTPYEA
                     LEFARNAYKNGYDVIIAAAGKAAHLPGVLAGCTTVPVIGLPIKSSELDGMDALLSIVQ
                     MPPGVPVATVGINAAENAALLAIQILSVKYEDLRNKFLDYKKQMAEKVFEKDKKLNEK
                     LMSS"
     gene            complement(2273..>3147)
                     /locus_tag="PLD67_06310"
     CDS             complement(2273..>3147)
                     /locus_tag="PLD67_06310"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HOT22167.1"
                     /translation="KDGAGEIENYSFNSKHIAKSSISIAKPRVFIPVFPGTNCEYETK
                     RAFERAGAKADIFVFKNLSSQDIKDSITEMNSLIRQSQIIAFPGGFSAGDEPEGSGKF
                     IAAVFRNELLKDAVTDLLNNRDGLIIGICNGFQALIKLGLVPFGEITDMENDYPTLTY
                     NKIGRHIATFVKTKVVSNLSPWLRMCSVGDTHYLPVSHGEGGFAANQKWIEKLKTNGQ
                     IATQYVDLNNNPTYDGFYNPNGSVEAIEGITSPDGRVFGKMAHSERLDENLYKNIPGN
                     KNQPIFESGVKYFK"
BASE COUNT          832 a          720 c          470 g         1125 t
ORIGIN      
        1 ggaaaaataa aggttttgca ccctggcata taacatcatt tacacacatg gctaccgcgt
       61 cttcgccgat agtgtcgtgc ttgtctgcca taaatgcaat catgagtttt gttcccacac
      121 cgtctgttcc agaaactaat accggctctt cgtattcatc cttatcaatt tcaaacattc
      181 ctccaaaacc gcctatgttt gaaatcacac cctttatatg tgtttttctt acatgctcct
      241 taaagagctt taccgtttca tagccggaat ttacatccac gcctgaatct ttataagtca
      301 gtttatcagt cataatacct ccttatatac cgttattggg tacttccatg ggataatcgc
      361 cgtttaaaca tgccttacag tagccaagat ttgaacctgt agatttgatt aaattttcta
      421 aacttaaaaa atgtatgcta tctgctttaa tataatcttt tatttcttct attgttttat
      481 ttgcacctaa caattctttt ctgtctgggg tatttatgcc gaagaaacaa gaatgggtta
      541 ccggaggtga agctattcgt acatgaacct cttttgcccc tgcttcccta accatattaa
      601 caatttgttt tgtagttgtc cctcttacta tgctgtcatc aaccatgact acaactttat
      661 cctgaacaat ttcctttaaa atgcttagtt ttgttttgac tccctgctgt cttaatttat
      721 ctgtgggctc aatgaaagtc ctctttgcat atctgttttt tataattcca agaccatatg
      781 gaatacttaa ttcttctgca tatcccaatg cagccggtgt tcctgagtca ggcacgcaaa
      841 ataccagatc tgctttcacg ggagcttcct gtgccaacaa tctacctgat ttatatctcg
      901 tctcataaac atttacacca tctaaattgc tgtccggtct tgcaaaatat acatgctcaa
      961 atatacacaa tctttgttgt gcatatttag tggtttttat gctgttaact ccttcttcat
     1021 ttattacaac aatctcaccc ggctcgatat ctcttataaa ttcaccgccc attgcagtaa
     1081 ttccgcagct ttccgaagca agcaaatatc cgtcttccct tttcccaagg caaagaggtc
     1141 taaggccata gttgtccctt acgccgataa gctctttatc tgtcataact acaaatgcat
     1201 atgctccttt tacaacctcc attgtagttt gtatagattt aattatactg cctctgtaat
     1261 gtcttgaaat taaattggct acaacctcag aatcagtgga agtctgaaat attactcctt
     1321 cattttcaag tatttctctt atagaatctg cattaaccaa atttccgtta tgggctaagg
     1381 ctatcgaacc ttctttatat cgcactacta agggttgtgc attttgcttt tcgctttctc
     1441 cggatgtgga atatctgtca tgccctattg caatattacc ttttaaacta tctattacct
     1501 tttgcgaaag aacatcggca accaatccca tatccttatg aaattcaatt ctttttcctg
     1561 tatgaattgc aatacctgca ctttcctggc ctctatgctg aagtgcaaat aaaccgtaat
     1621 atataagctg acttgtaata tccggattgt ctgaatatac tccaacaacc ccgcattcct
     1681 catgtaattt atcttcttct aaattgaaca tagtatctcc ttcttgtcat tggtatgaat
     1741 gtttgtctcg tcatctttat cctgtgctca ggatgacatc aatttttcgt ttaatttctt
     1801 gtctttttca aatacttttt cagccatttg ttttttataa tccaaaaact tattccttaa
     1861 atcttcgtat ttcaccgata atatctggat agccagtaat gcagcatttt ctgcggcatt
     1921 tatgcccact gttgcaaccg gcactcccgg aggcatctgt actatcgata acaaggcatc
     1981 cattccgtct aattccgaag atttaatcgg aagtcctatt accggcacag tcgtgcatcc
     2041 tgccaacact cccggcaaat gggcagcctt tcctgctgca gctataatga catcataacc
     2101 gtttttatat gcatttcttg caaattccaa agcttcatat ggtgttctgt gggctgatat
     2161 aacccttaca tcagccataa cttcgaaatc tttcaaaact ttataaccct tttcaactac
     2221 cggaaagtct gaatcacttc ccataactat tgctactttc attactcctc cattatttaa
     2281 aatattttac gcctgattca aatatcggtt ggtttttgtt tccgggaata tttttatata
     2341 aattttcgtc cagtctttca gaatgtgcca tcttgccgaa aacccttccg tcagggcttg
     2401 ttattccttc aattgcttcc acagagccgt tcggattgta aaaaccgtca taggtcggat
     2461 tattatttaa atcaacatat tgagttgcta tttgaccatt tgttttcaat ttttctatcc
     2521 atttttgatt tgctgcaaat cctccttctc cgtgagacac aggtaagtag tgggtgtctc
     2581 ccacactgca cattcttaac cacggggata ggttgcttac aacctttgtt ttaacaaatg
     2641 ttgctatatg ccttcctatc ttattataag ttaaagtcgg ataatcattc tccatatcag
     2701 tgatttcgcc aaatggtacc aatcccaatt taatgagtgc ctggaagccg ttgcatatac
     2761 caataatcaa gccgtctcta ttgtttaaca aatctgtaac tgcatccttt aacaattcat
     2821 tcctgaatac tgccgcaata aactttccgg agccttcagg ttcatctccg gcactgaatc
     2881 ctcctggaaa tgcaataatc tgtgactgcc ttatgagtga attcatttct gttattgaat
     2941 ctttaatatc ctgtgagctt aaatttttaa aaacaaaaat atctgctttt gctccggctc
     3001 tttcaaaggc tctcttggtt tcatattcgc aatttgttcc cgggaatacc ggaataaata
     3061 ctcttggttt tgcaatggat atggaggatt tagcaatatg tttagaatta aaactatagt
     3121 tctcaatctc tccggctcca tcctttt
//
LOCUS       DAOSOW010000220         3139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_30987_length_3139_cov_3.313554, whole genome shotgun sequence.
ACCESSION   DAOSOW010000220 DAOSOW010000000
VERSION     DAOSOW010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30987_length_3139_cov_3.313554"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="PLD67_06315"
     CDS             complement(<1..324)
                     /locus_tag="PLD67_06315"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT22168.1"
                     /translation="MEIITSYENLQELIEKNDMVMIYFGSKTCSVCKSMTPKVENMIK
                     RYPNIYAIMVEADKSLDISSKFNVFTVPVILLYIEGKETIREVRIISLDNLEQKIARY
                     CQIFYV"
     gene            476..775
                     /locus_tag="PLD67_06320"
     CDS             476..775
                     /locus_tag="PLD67_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT22169.1"
                     /translation="MIDFSNKERVKKVLSEKAELFKSIAHPVRLCILAMLIKEEESNV
                     TDIQCCLDVPQPTVSQHLSKLKSAGILSAERNGTEIYYKIINEEVKKIVSQILDA"
     gene            786..>3139
                     /locus_tag="PLD67_06325"
     CDS             786..>3139
                     /locus_tag="PLD67_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT22170.1"
                     /translation="MSKKILIIGGVAGGASAAARLRRLDETSKIIMFEKGPYISYANC
                     GLPYHIGKVIKERSSLLLQTPEAMSKKYNIDVRVESEVISINRESKTLDVKNLKTGEI
                     YTESYDTLIISTGSSPLKPPIEGINSPGIFTLWTVPDTDNIIKFINEKKPKNALVIGG
                     GFIGLEVAENLAAAGLNVTVAEAMNQVMAPIDYEMAQLVHENMVSNNIELILGDGVSK
                     FESNDGVITITLASGRTLTADMVILSIGVRPNGQLAKEADLEVNQRGGIIVDKYLKTS
                     DPSIYAIGDVIEVEEFINKGKAMIALAGPANKQGRICANNIYGAEEEYKGTQGTSVAK
                     VFDLTVASTGTNEKTLNRMGMVEGKDYYTAIISQKSHAGYYPESAEMILKLIFSTDGQ
                     KIFGAQIIGEDGVDKRIDTIATTIGLKGSIYDLKELELAYAPPYSSAKDPVNMLGFVA
                     ENIIQNKVKFSKWNDIDPSKQNEYTLLDIREEAEHMVFDIPRTVNIPLGQLRGRINEL
                     NKDDNIIILCSIGVRSYNAARVLMENGFKNVLVYPAGTNFYKSVYYNYFNETGRESND
                     DTLVVSNTNEKKTDTTVKIKSSIQVDCSGLQCPGPIMKVYETMKNANDGDIVQVSATD
                     SGFAKDVEAWSRRTGNTLLKIEKDGKNNIVYLQKGIDNKKAETALEKTGLQPSNDGKT
                     IIVFSGDMDKVLASFVIANGAAAMGRPVTMFFTFWGLNVLRKTEKQNAKKPFIDTMFG
                     KMMPRGVNKLKLSKMNMGGLGTAMMRKVMKDKNIDSLEDLIKKA"
BASE COUNT         1152 a          494 c          598 g          895 t
ORIGIN      
        1 aacataaaat atctggcaat accttgcaat tttttgttct aaattatcca agcttattat
       61 tcttacttcc ctgattgttt ctttaccttc aatatataat agtattaccg gcacggtaaa
      121 cacattaaat ttggaagata tatctaaact cttatccgct tcaaccatta ttgcataaat
      181 attaggatat cttttaatca tattctccac tttaggtgtc atggatttac ataccgagca
      241 tgtcttgctt ccaaaataaa tcattaccat atcgttcttt tcaataagct cttgtaaatt
      301 ttcataagat gttataattt ccatagtact cctcattatt aatgttctta tttacatttt
      361 accattattt cataaaatta accatttttt taaaaacaat aaagtttttc ttgcgtatta
      421 tattattata tgctaatatt ctaatatatt aatattgcaa tatatggagg gtaaaatgat
      481 agatttcagc aataaggaaa gagttaaaaa ggtattgtcg gaaaaagcag agctgtttaa
      541 atcaatagca catcccgtaa gactttgcat attggcaatg cttataaaag aagaggaaag
      601 caatgttacc gatattcaat gctgtttgga tgttccgcag cctactgtat cacagcatct
      661 ttccaaactt aagtcagcag gcatactttc agcggaaaga aacggaacgg aaatttatta
      721 taaaattata aatgaagaag taaaaaaaat agtaagccaa attttagatg cataagggag
      781 gatttatgag caagaaaatt ttaataatag gcggtgttgc aggaggagca tccgcagcag
      841 ccagacttag aagacttgat gaaacatcaa aaattataat gtttgaaaaa gggccttata
      901 tatcgtatgc aaattgcggt ttgccttacc atataggaaa agttataaaa gaaaggtcat
      961 ccttattgtt gcagactccc gaagctatgt caaaaaaata taatattgac gtgcgtgtag
     1021 agtctgaagt tatttcaatt aacagagaga gcaaaaccct agatgttaag aatctaaaaa
     1081 ccggcgaaat ttatactgaa agctatgata ccttgattat atccacgggc tcatcgccct
     1141 taaaaccgcc gattgaagga attaattcac cgggtatttt taccttgtgg actgtgcccg
     1201 atacagataa tataataaaa ttcataaatg aaaagaaacc taaaaacgct cttgtaatag
     1261 ggggtggttt catagggctt gaggttgcag aaaatcttgc ggctgcagga ttaaatgtaa
     1321 ctgttgccga agcaatgaac caggttatgg ctcctataga ttatgaaatg gcacaattag
     1381 tacatgaaaa catggtatca aataatattg agttgatttt aggagatggt gtatctaaat
     1441 tcgaaagcaa tgacggcgta ataaccataa cattggcaag cggcagaacc ttaacagcag
     1501 acatggttat attgtccatc ggtgtccgcc caaacggtca gctggcaaaa gaagcagacc
     1561 ttgaagttaa tcaaagaggc ggaataattg tagataaata tctgaaaaca tccgatccaa
     1621 gtatatatgc aatcggtgat gttattgagg ttgaagaatt tataaacaaa ggcaaagcaa
     1681 tgattgccct tgcaggtcct gccaacaagc aaggaagaat atgcgccaac aatatttatg
     1741 gtgctgaaga agaatataaa ggtacccagg gaacttcagt ggccaaggtt tttgatttaa
     1801 ccgttgcatc gacaggtacc aatgaaaaaa ctctaaatag aatgggaatg gttgaaggca
     1861 aggactatta tacagcaatt atatcacaaa aatctcatgc aggttattat cctgaatctg
     1921 ctgaaatgat attaaaatta atattttcaa cagacggaca aaaaatattc ggtgcccaaa
     1981 ttataggaga ggacggagtt gataaacgaa ttgatacaat tgcgacaaca attggtctga
     2041 agggcagtat ttatgattta aaagagcttg aacttgctta cgcacctcca tattcctcag
     2101 ctaaggaccc tgtcaatatg cttggatttg tggctgagaa tattattcaa aacaaggtta
     2161 aattcagcaa gtggaatgat attgaccctt caaaacaaaa tgaatacaca ttgcttgaca
     2221 tacgagagga agcggagcac atggtatttg atatacccag aaccgtaaat attcctttgg
     2281 gccagcttcg tggcagaatt aacgaattaa ataaagatga taacataatt atactttgct
     2341 ctataggggt tcgctcatac aatgcagcca gggttttaat ggaaaatgga tttaaaaatg
     2401 ttttagttta tcctgcagga acaaactttt ataaatctgt ttattacaac tatttcaacg
     2461 aaaccggaag agaaagtaat gatgacacat tagtagtaag caatacaaat gaaaagaaaa
     2521 cagatacaac agtaaaaatt aaatcatcta ttcaagtgga ttgcagcgga ttacagtgcc
     2581 cgggccccat tatgaaggta tatgaaacaa tgaaaaatgc aaatgatgga gatattgttc
     2641 aagtatctgc aacagattca ggatttgcta aagatgttga agcatggagc agaagaacag
     2701 gcaataccct tctaaaaata gaaaaggatg gtaaaaataa tattgtatat ttgcaaaaag
     2761 gaatagacaa taaaaaagct gaaacagctt tagaaaaaac aggacttcaa ccgtctaatg
     2821 acggtaaaac cataattgta ttcagcggcg acatggataa agttctggca tcctttgtta
     2881 ttgcaaacgg tgctgcagca atgggaagac cggttactat gtttttcaca ttctggggac
     2941 ttaatgtttt aagaaaaacc gagaagcaaa atgctaaaaa acctttcatt gataccatgt
     3001 tcggcaaaat gatgcctcga ggagttaaca aactaaaact ttcaaaaatg aacatgggcg
     3061 gattgggaac agctatgatg cgaaaagtaa tgaaggataa aaatatcgat tcattggaag
     3121 atttgattaa aaaggcaat
//
LOCUS       DAOSOW010000221         3137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_31013_length_3137_cov_3.572031, whole genome shotgun sequence.
ACCESSION   DAOSOW010000221 DAOSOW010000000
VERSION     DAOSOW010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31013_length_3137_cov_3.572031"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="PLD67_06330"
     CDS             <1..280
                     /locus_tag="PLD67_06330"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOT22171.1"
                     /translation="FEDIGLVYKHNFDDGFSRYEILSQNSNEIHQHHHLLCKQCGKII
                     EVKEDLMNTLEEIIEKQYNFEILNHVVKFTGICAQCRDKENKDGRKET"
     gene            261..1937
                     /locus_tag="PLD67_06335"
     CDS             261..1937
                     /locus_tag="PLD67_06335"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004396470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HOT22172.1"
                     /translation="MAEKKHKLKVIPLGGLGEIGKNITVIEYHNDIIVIDSGMAFPGD
                     EMLGIDIVIPDITYLIKNKEKVKGIILTHGHEDHIGSIPYFLKKLNIPIYGTKLTLGL
                     VENKLLEHKIEDASLNPINAGDTLKLGCFKITPIRVNHSIPDSVGFAIDTPIGTIVHT
                     GDFKIDYTPINEDVIDLGKFADIGRKGVLLAFSDSTNVERPGFTESEKTIGQKFMDIF
                     KNCDSRIIVASFASNIHRLQQVIDAAVVNKRKVAISGRSIINAIKVSLELGYLNAPQG
                     TIINVNELNSLKDDEIVILTTGSQGEPMSALTRMANNDHKKIQIKEGDLVIISANPIP
                     GNEITVSRVINMLYEKGAKVLYDALTQVHVSGHARRDELKLMLTLLKPKFFIPVHGEY
                     RHLVQHAKLANEIGIPINNTVIGSNGDVIELTSKSIVKNNTVTSGNILVDGLGVGDVG
                     NIVLRDRRLLSENGLIIVVLSTDRSTGKILAGPEIVSRGFIYVRENIDLIEESKTVVR
                     KALEKCEDTNVKEWNNIKMVIKDSLGSFIYEKIKRTPMILPIIVEVDLEV"
     gene            2166..2867
                     /locus_tag="PLD67_06340"
     CDS             2166..2867
                     /locus_tag="PLD67_06340"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOT22173.1"
                     /translation="MINNETIEAERTRVCLTIGRIAPDFTAVTTEGPITLSQYRGKWV
                     LFFSHPGDFTPVCTSEFIAFAQLQPEFEKRNVQLLGISIDSNPAHLGWLYDIFQMTGI
                     VMPFPLIADRDASIAALYGMVSPDRIYEQSVRDVFIIDPNQRIRSILIYPATNGRNMY
                     ELLRLIDALQVSTTYGVSTPANWMPGDPVIVPVASTLDEAIERGMDPESLELNCLAWW
                     YCFTDLSELTPLPNP"
BASE COUNT         1102 a          517 c          627 g          891 t
ORIGIN      
        1 gtttgaggat ataggacttg tatataagca caacttcgat gacgggtttt ccagatatga
       61 aatattatca caaaacagta atgaaattca tcagcaccat catttattgt gcaaacaatg
      121 cggcaaaatc atagaggtta aagaagacct tatgaacaca ttggaagaaa taatcgaaaa
      181 acaatacaat tttgaaatat taaatcatgt ggtcaaattc accggaatct gtgcacaatg
      241 cagagataag gagaataaag atggcagaaa agaaacataa attaaaagta ataccattgg
      301 ggggactcgg tgaaatcgga aaaaacataa ccgttattga atatcataat gatattatcg
      361 taatagacag cggaatggct tttccgggag atgaaatgct tggtattgac atagtgatac
      421 cggatattac ttatttaata aagaacaaag aaaaagtaaa gggtattata ttaactcatg
      481 gtcatgaaga ccatatcggc tcaatacctt actttttaaa aaagctaaat ataccgattt
      541 acggaacaaa acttacctta ggtcttgttg aaaataagct tttagaacat aaaatagaag
      601 atgcatcctt aaatcccatt aatgctgggg atacattaaa attaggatgt tttaaaatta
      661 cgcctataag agttaatcac agtatacctg attcagtggg ctttgccata gatacaccta
      721 tcggtacaat agtccatacg ggagatttta aaattgatta tacacccatc aatgaagatg
      781 ttatcgattt aggtaagttt gcggatattg gaagaaaagg cgttcttctt gccttttctg
      841 acagcactaa tgttgaaagg cctggcttca cggaatcaga gaaaacaatt ggtcagaaat
      901 ttatggatat atttaaaaac tgtgattccc gaataattgt tgcgtctttc gcttcaaata
      961 ttcataggct gcagcaggtt atcgacgcag cggtcgttaa caaaagaaag gttgcaatat
     1021 caggtagaag cataataaat gcaattaaag tttctctgga attaggatat ctgaatgctc
     1081 cacagggaac aataataaat gtaaacgagc taaacagcct taaggatgat gagattgtta
     1141 tattgactac aggaagccag ggagagccta tgtctgcctt gacaagaatg gctaacaatg
     1201 accataaaaa aattcaaata aaagaaggag acttggttat aatttcagcc aatccaatac
     1261 ccggaaatga aataacagtt tcaagagtaa tcaatatgct ctatgaaaaa ggcgcaaagg
     1321 tgctttatga tgcactgaca caagtgcatg tatcaggcca cgccagaaga gatgaattga
     1381 agcttatgct tactctgtta aaaccaaagt ttttcatacc agttcacggc gaatacagac
     1441 atttggtgca gcatgcaaag ctggcgaatg aaataggcat tcccattaat aatactgtaa
     1501 taggcagcaa tggtgacgta attgagctta cttccaagtc aatagttaaa aacaacactg
     1561 ttacatccgg aaatattctt gtagacggct taggagtggg ggatgtcggg aatatagtcc
     1621 ttcgagacag aagacttctt tcagaaaatg gtttgataat agttgtacta tctaccgata
     1681 gaagtacggg aaaaatttta gccggtcctg aaatagtatc gagaggattc atatatgtac
     1741 gcgagaatat agatttaata gaagaatcaa aaacagtggt aagaaaagca ttggaaaagt
     1801 gtgaagatac aaatgttaaa gaatggaaca atattaaaat ggtaataaag gattccttag
     1861 gcagctttat ttatgaaaaa ataaaaagaa ctccaatgat acttcccatt atagtagaag
     1921 ttgatttgga agtataaatt tagggacaca aaataaatag ggacttaacg tccctatttt
     1981 tgttaagaac attggtgcca ggcaccatca accaccatgg tgccaaacca ccgtggtgcc
     2041 aggcaccagg aaatactgaa cgcagtgaag aatctcataa ccatttatag tgtaatagat
     2101 tcgaactata tcatttgtcc tttcatataa taaataaaat acatatggga ggtaacaaac
     2161 aaattatgat caataatgaa actatagaag ccgaacgtac aagggtatgc ctgacaatcg
     2221 gaaggattgc acctgacttt acagccgtta ctacagaggg accaataacc ctgtctcaat
     2281 atagagggaa gtgggttttg tttttcagcc atccgggaga tttcactcct gtttgcacat
     2341 cagaatttat tgcatttgct caacttcaac ctgaatttga aaaaagaaat gttcagctct
     2401 taggaataag cattgattca aatcctgcac accttggctg gctctacgat atattccaaa
     2461 tgacaggtat agttatgccc tttcctctta ttgccgacag ggatgcaagt atagcagcgc
     2521 tttacggcat ggttagtcct gacagaatct atgagcaaag tgttagagat gtttttatta
     2581 tagacccaaa tcaaagaata aggtcaattc tcatctatcc tgcaactaac ggcagaaaca
     2641 tgtatgaatt gttaagatta attgatgcac tgcaagtgtc aacaacatat ggtgtaagca
     2701 cccctgcaaa ctggatgccg ggggacccgg taattgttcc tgttgcaagc accctcgatg
     2761 aggcaattga aagaggcatg gatcccgaaa gtttagaact gaactgtctt gcatggtggt
     2821 attgttttac ggatttatct gaactgacac ctttaccaaa tccataatat gtcgataaaa
     2881 tccttcacta agttcaggaa gacatacaat aagtccctat ttatttttta gttgaaaaaa
     2941 tggggtaaaa aaacaaccgg tgagctttta ggtcaccggt tattaatgct tattatcttt
     3001 tgatgttgtg aaagtttact cctaagattg gtctgaactg ggatggtttg ttgtttacaa
     3061 tacttttatc ttccattctt tgatttgacc tttttgaagt caaatttaga tttggggtta
     3121 ttctaccgtt gatagca
//
LOCUS       DAOSOW010000222         3116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_31302_length_3116_cov_3.766743, whole genome shotgun sequence.
ACCESSION   DAOSOW010000222 DAOSOW010000000
VERSION     DAOSOW010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31302_length_3116_cov_3.766743"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1808)
                     /locus_tag="PLD67_06345"
     CDS             complement(<1..1808)
                     /locus_tag="PLD67_06345"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOT22174.1"
                     /translation="MMLKRIAAISAAAILLLSSSAYGSSLYTVYDLSEEIRLSKSITY
                     ERIEKYTSSGWMNINVIRADLTDEHTEIKPINNEDGISNRSTLSSMIRSSGAVAAVNG
                     DFFYMGDPTYTYGPIIRDNKLITSPLPFNDGYPTVSKLKDEIDISVWNPKITLYGSDS
                     TEFNVVVMNKTSNIEWGPTILTSDWNKTSPGYTTQDIVEVVVVDNIVSSVRKNQPSTI
                     IPENGYIVASSNPATMELMLNSFKEGHPVSLNIELDFSPEDIDWSFGALNYLVKDGRE
                     NVISSQVLGVNPRTAIGFNKDNTEIILVTVDGRHRDYVGAKQTELAKIMIELGAYNAV
                     NMDGGGSTTMAVDFLRNANVTVVNFPSDGRERKIASGVGVFNNYPESNNVDKIEITAS
                     QNTVFNKTETNLDLKFYNEYYTPVEFDLKNVDYTVSPAGAGKVVNNVFMPEKPGKATI
                     TAHAGNAEGHIEIDILDKPVALKLDTDSLTLGFNDKYELGTVLGIDKDGNSAYIPWKY
                     ITASYRNRVGKIEDGVFISSDIANTGAVTLTFGDAIKHIQVKVGYRYKTLNRFENLDN
                     LKLSLYPEESAGSISITKDFVKEGKNALRLDYDFTK"
     regulatory      2126..2221
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2386..>3116
                     /gene="metK"
                     /locus_tag="PLD67_06350"
     CDS             2386..>3116
                     /gene="metK"
                     /locus_tag="PLD67_06350"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003445795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOT22175.1"
                     /translation="MRKWLFTSESVTEGHPDKMCDQIADGILDAIMEQDPNGRVACEA
                     ITKTGFVLVAGEISTSCYIDIPHLVRETIKNIGYDNALYGFDYKTCAVFTSIEEQSQD
                     IALGVNEADEYKKNNNDINDMIGAGDQGMMFGFACNETPELMPMPISLAHKLAKKLAE
                     VRKNNIVDYLRPDGKTQVTVEYHDNKPVRIDTIVISTQHNPEVNIDTIKKDLIDIVIK
                     PTVPDELLDENTKYIVNPTGRFVIGG"
BASE COUNT         1001 a          555 c          544 g         1016 t
ORIGIN      
        1 atttttgtaa agtcgtaatc caatcgaagt gcatttttcc cttcttttac aaagtccttt
       61 gtaatggata tagaacctgc cgattcttca ggatacaagc ttaattttaa gttatcaaga
      121 ttttcaaaac gatttaatgt cttatatctg tatcctacct taacttgaat atgttttatg
      181 gcatctccaa aggttaaagt taccgctccg gtatttgcaa tatcacttga tataaatact
      241 ccatcttcaa tttttccaac cctgtttcta taggaagcag taatgtactt ccatggtata
      301 tatgcggaat tgccgtcttt atcaattcca agaaccgtgc ctagttcata tttatcatta
      361 aatcctaggg ttaatgaatc agtatccaac ttcaatgcca ccggcttgtc taatatatct
      421 atctcaatat gtccttctgc attcccggca tgagcggtaa tcgttgcttt tcccggtttt
      481 tcaggcatga atacattatt tacaacctta cctgcaccgg caggagaaac agtgtaatct
      541 acatttttca aatcaaattc tacaggagta taatattcat tgtaaaactt taaatctaaa
      601 tttgtttctg ttttattaaa taccgtattt tgtgatgctg ttatttcaat tttatcaaca
      661 ttgttactct caggataatt attaaatact ccgacacccg atgcaatttt tctttctctt
      721 ccgtctgacg ggaagttaac aacagtaaca tttgcatttc ttaaaaagtc aacagccatg
      781 gttgtggagc ctcctccatc catattaaca gcattgtaag cgcctaattc tatcattatt
      841 tttgcaagtt ctgtctgttt tgcacccaca taatctcggt gcctcccgtc aacagttacc
      901 aaaattattt ccgtattgtc tttgttaaaa ccgattgccg ttcttggatt tactcctaag
      961 acctgcgaac ttatcacatt ttccctgccg tctttaacca agtagttcag tgcaccaaaa
     1021 gaccaatcaa tatcttccgg gctaaaatcc aactcaatat ttaaggatac gggatgacct
     1081 tccttgaatg aattaagcat taattccatt gtagcaggat ttgagctagc aactatatac
     1141 ccgttttccg gaataatcgt agaaggctga ttttttctta ctgatgatac gatattatca
     1201 actacaacaa cttcaactat atcctgtgtt gtatatccgg gagatgtttt attccagtcg
     1261 gatgtgagaa ttgtcggacc ccattcaata tttgaagttt tattcattac aacaacgttg
     1321 aattccgtag aatccgagcc ataaagggtg attttggggt tccatacgga aatatcaatt
     1381 tcatctttta attttgaaac ggtgggatat ccgtcattaa aaggaagagg agaagttatt
     1441 aatttattgt ctctgattat tggaccgtat gtatatgtgg ggtcccccat gtagaaaaag
     1501 tctccgttaa cagctgcaac cgctcccgat gacctaatca tggagcttaa agttgatctg
     1561 tttgaaatgc cgtcttcatt gttaataggt ttaatttctg tatgctcatc ggttaaatct
     1621 gcccggataa cattaatatt catccaccct gatgatgtat acttttcaat tctttcatat
     1681 gtaatactct ttgagagtct aatttcttca gataagtcgt aaacagtata aagactgctg
     1741 ccgtatgctg aagatgataa taatagtatt gctgcagcag agatggctgc tattctttta
     1801 agcatcatgt taatcattcc tttctttttt aaattataac ataagtgcaa gacgaatgcg
     1861 cttgcttcgc accgatacaa tgagaacatt aaattcaaaa agacttagta caagtttcat
     1921 cttgtattca ttttatcaga attcacccat aattgccatt aatttaagga tattagagca
     1981 tgttaaaata tagcaaatca gggttttatt tatctattat agacgtaatt ataataatat
     2041 agttccatat aatcaataat aatagtaata cgataaaaat ttaaacgttg acaataaaaa
     2101 acggatgtga taatattaaa aaaaatcctt atcaagagtg gtggagggac gaggcccaat
     2161 gaaacccggc agcctgatta ttcaaggtgc caattcccgc ggattttccg gaagatgagg
     2221 ctataggaaa ctatataacc tcttcttgtg aagaggtttt ttagtgataa attgttgata
     2281 gcctatacgg ccgttcaaat tttcgagacg aatttattgc attcattacg tgcaaaatat
     2341 taaaggtatg caaaatttaa atacaaaaaa ggaaaggata aaataatgag aaaatggtta
     2401 tttacatctg aatctgttac agaaggtcat cccgataaaa tgtgcgatca gattgcagat
     2461 ggcatcttag atgcaattat ggaacaagat cctaatggaa gggttgcctg cgaagcaatt
     2521 actaagaccg gatttgtact tgtagcagga gaaatcagca cgagctgtta tattgatatt
     2581 ccgcatttag taagagaaac tataaagaat atcggttatg acaatgcctt atatggattt
     2641 gattataaaa catgtgctgt attcacatca attgaagagc aatctcagga catagcattg
     2701 ggtgttaatg aagcagatga atataaaaag aataataatg atataaatga tatgattgga
     2761 gcaggggacc agggtatgat gtttggtttt gcctgcaatg aaacacctga attgatgccc
     2821 atgccgatat ccttggcaca taagctggca aagaagcttg cagaggtaag gaaaaacaat
     2881 atagtggatt acttaagacc ggacggaaaa actcaggtta ctgttgaata tcatgataat
     2941 aaacctgtta gaattgacac aatagttata tctacccagc ataatcctga agttaacata
     3001 gatacaataa aaaaagacct tattgacatt gtaataaaac caaccgttcc cgatgaattg
     3061 ttggatgaga atacaaaata tatcgtaaat cccaccggaa gatttgttat aggcgg
//
LOCUS       DAOSOW010000223         3096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_31561_length_3096_cov_3.401513, whole genome shotgun sequence.
ACCESSION   DAOSOW010000223 DAOSOW010000000
VERSION     DAOSOW010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31561_length_3096_cov_3.401513"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="PLD67_06355"
     CDS             <1..120
                     /locus_tag="PLD67_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOT22176.1"
                     /translation="EAAGQTVMHLHIHLIPRYSGDIKNPRGGVRGVIPDKRIY"
     gene            143..418
                     /locus_tag="PLD67_06360"
     CDS             143..418
                     /locus_tag="PLD67_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22177.1"
                     /translation="MKRKTKKINNHYVVDEIINHDENGYSGEAIELLAKFENYYEDLV
                     SRQEAIIKEMDKLKAENKTNTVKFKQLFANKLNNSANLLILKQFGIH"
     gene            452..2296
                     /locus_tag="PLD67_06365"
     CDS             452..2296
                     /locus_tag="PLD67_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22178.1"
                     /translation="MANNKNKEKEYQPIKLLKNRIYPTYQLYAVINSKVSAEEAMVIA
                     VLETFSWLRKRFRELTIPDEIDYPEPDKYKSVRLDDFKSFRINEGYVVDVVFIKEKGV
                     FAFQLVEPDLGPEPGNENQGRKPAPGRVFATNIAYRIYNGKLECGFKTVCSEPENTKA
                     ACEVFRLAVIRSIVLNELLGLDQIIPIIRDMHQINSIDKVDRFIDYIRSNERQLPFVL
                     TVEYKKPVDFNTLVKTKPNFDINNLLNRKIEDLIATTKVEDKEEIFEDRMRELAGKKM
                     GYAQFGFLPYKYINNFNDKLGCNISGGDVCVFYPERYNHINIIKRDSIKLNEKKFLKD
                     LENELQEFPKYKNINFGNVIFINEARIEELNKIIELSNSKEEIIKAIEDKIEIIEQKY
                     EDKIAVLNNEMHEKDEKIDTLKAEIKELNDKFDKFYLQVEKLKSDYNDQINKLNLKIE
                     WKDNKLKQPRKTEDIPEWVEKQFAGKLIFHNRAIGEIKKTPTGEIDMSLLCDAIEYLA
                     NEYRDELLGLISEVESNNICSDKYNRSFVVAPTGDKSIEMYTKEYKVKYKKGPAEKSR
                     EVLLDQHLKVGKGNKDNLRIYFFYDEEKDLIVVGSLPKHLSSVNKKKD"
     gene            2396..>3096
                     /gene="panB"
                     /locus_tag="PLD67_06370"
     CDS             2396..>3096
                     /gene="panB"
                     /locus_tag="PLD67_06370"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOT22179.1"
                     /translation="MAKKKTILDLYKMKENGEKAVWITAYDCCFAAYAEKAGMDMILV
                     GDSMGMIVYGYQGTTPVTMDMCINHCQAVRRGAPNTYIIGDMPFGSYHESVEDAVRNA
                     VRFIKEADVDAIKLEGGVSVADKIKAIAQAGIVVFGHVGLTPQSSAALGGFKAQGRTT
                     ESARAVIEDAIAVYEAGAKFILLEGVPEEVCAFVHKILPIPVYGIGAGADTDGQVLVI
                     GDALGMFEAFTPKFT"
BASE COUNT         1162 a          389 c          662 g          883 t
ORIGIN      
        1 gaggcagcag gacagacagt aatgcacttg catattcatt taataccaag atacagcgga
       61 gatataaaaa atccgagagg tggcgtaaga ggagttatcc ctgataaaag aatttattga
      121 ttatatataa ggatgtaata atatgaaaag aaaaactaag aaaataaaca atcattatgt
      181 agttgatgaa attattaatc atgatgaaaa cgggtattca ggcgaagcaa ttgaattgct
      241 tgctaagttt gaaaactatt atgaggattt agtttcaaga caggaagcaa taattaaaga
      301 aatggataag ctaaaggctg aaaacaaaac aaatacggta aagtttaaac agctttttgc
      361 aaataagcta aataacagtg ctaacttgtt gatattgaaa caatttggaa ttcattaata
      421 aatcagtgtt tctattgaag gagtggggga tatggcaaat aataaaaata aagaaaaaga
      481 atatcaaccg attaagctgc taaaaaatag aatttatcct acatatcagc tttatgctgt
      541 tataaacagc aaggtatctg ctgaagaagc aatggttata gctgtgttag aaactttttc
      601 gtggttaaga aaacgtttta gagagttaac tatacccgat gaaattgatt accctgaacc
      661 tgataaatat aaaagtgtta gattagatga ctttaaatcc ttccgcatta atgaaggtta
      721 tgtggtggat gtggttttta tcaaggaaaa aggtgtcttt gcatttcagt tggttgagcc
      781 tgatttagga cctgaacccg gaaatgaaaa tcaaggacga aaaccggcac ccggcagagt
      841 atttgctacc aatatagctt atagaattta taacggaaaa ttggagtgcg ggtttaagac
      901 cgtctgctct gagcctgaaa atacgaaggc tgcttgcgag gtgtttagat tagccgttat
      961 taggtctatt gttttgaatg agctgttggg actggaccaa attattccca ttataaggga
     1021 tatgcatcaa atcaacagca ttgataaggt tgatagattt attgattata tcagaagcaa
     1081 tgaaagacag cttcctttcg ttttgactgt cgaatataaa aagccggttg atttcaatac
     1141 tttagtaaaa accaaaccga attttgacat taataatcta ttgaaccgca aaattgaaga
     1201 tttaattgca actacgaagg ttgaagataa agaagaaata tttgaagata gaatgagaga
     1261 attggcaggt aaaaaaatgg ggtatgcgca gtttggattt ttgccttata aatatattaa
     1321 taattttaat gataaactcg ggtgcaatat ttcaggcggt gatgtatgtg tattttatcc
     1381 tgaaaggtat aatcatataa atataattaa acgtgatagt attaaattaa atgaaaagaa
     1441 attcttaaaa gatttagaaa atgaactgca ggaatttcca aaatacaaaa acataaattt
     1501 cggcaatgta atttttataa atgaagcaag aatcgaagag cttaacaaga ttattgaatt
     1561 aagcaattca aaagaagaaa ttattaaagc tattgaagat aagatagaaa ttattgagca
     1621 gaaatatgaa gataaaatag ctgtcttaaa taatgagatg catgaaaagg atgagaaaat
     1681 tgacacatta aaagcagaaa taaaagaact taatgacaag tttgataagt tttatctgca
     1741 agtagagaaa cttaagagtg attacaatga ccagattaac aagctgaatt taaaaattga
     1801 atggaaggat aataaactta agcagccgag gaagacggaa gatatacctg aatgggttga
     1861 aaaacaattt gccggaaaac ttatttttca caacagggca atcggtgaga ttaaaaagac
     1921 tcctacaggt gaaatagata tgagtttgct ttgtgatgct attgagtatc ttgcaaatga
     1981 atatcgcgat gaactattag gtttaatcag tgaggtggaa agcaataata tttgttccga
     2041 taaatataac agaagttttg ttgtagctcc cacaggcgat aagagtattg aaatgtatac
     2101 caaagaatat aaggtcaagt acaaaaaagg accagccgaa aaatcaagag aagtcttgct
     2161 tgatcaacat ctgaaggtag gtaaaggcaa caaggataat cttcgaatat atttctttta
     2221 tgacgaggaa aaagacttaa ttgtcgttgg ctccctgcca aagcatttaa gctcggttaa
     2281 taaaaagaaa gattagaaat cagaaaatta aaagaaaaag tggtcaaccg aaattgtatg
     2341 gtatataatt aaaaaaataa tatttagtaa actatgatga aagaggggtg ccgatatggc
     2401 taagaaaaaa actattttag acttatataa aatgaaagaa aatggtgaaa aggcagtatg
     2461 gattactgca tatgactgct gttttgcagc atatgcggaa aaagccggca tggatatgat
     2521 tttggtggga gattcaatgg gaatgatagt ctacggatat caaggcacta ctcccgttac
     2581 tatggatatg tgcattaatc attgtcaggc agtaagaaga ggagctccca atacatatat
     2641 aatcggtgat atgccatttg gctcatatca tgaaagtgtt gaggatgctg tgcgtaacgc
     2701 agtacgtttt ataaaagaag ctgatgttga tgccataaaa ttagagggcg gcgtttctgt
     2761 tgcagataaa attaaagcta tagcacaagc aggaatagtt gtatttgggc atgtcggatt
     2821 aactcctcaa agttctgctg ctttaggcgg gtttaaggct caggggcgta caacggaaag
     2881 tgcacgtgct gtaattgaag atgccatagc tgtttatgaa gcaggagcta aatttatatt
     2941 gttggaaggt gttcccgaag aggtatgtgc ttttgttcat aaaatattgc ctataccggt
     3001 ttatggaatc ggggcaggag ctgatacgga cggtcaagta ttagttatcg gcgatgctct
     3061 tggaatgttt gaagccttta cacctaaatt cacaaa
//
LOCUS       DAOSOW010000224         3094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_31588_length_3094_cov_3.350444, whole genome shotgun sequence.
ACCESSION   DAOSOW010000224 DAOSOW010000000
VERSION     DAOSOW010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31588_length_3094_cov_3.350444"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..635)
                     /locus_tag="PLD67_06375"
     CDS             complement(114..635)
                     /locus_tag="PLD67_06375"
                     /inference="COORDINATES: protein motif:HMM:NF025635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HOT22180.1"
                     /translation="MISSNKSTQDKLKLLYILDYIDIPLTNNEITNYILEYSMMDYFT
                     LQLLLSDLCEVKFIHLKPYNGSEYYSVSKSGKAALEMFGDKLPEYFINEVARNYSGIK
                     KHIKKQREIFGHYYKRKDDEFIVSLQVSENSTTIFNLSINVTDEIMAKKIIKRWETNP
                     EKIFGQIINALTN"
     gene            871..1959
                     /locus_tag="PLD67_06380"
     CDS             871..1959
                     /locus_tag="PLD67_06380"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOT22181.1"
                     /translation="MKKIIILIALIMMTGCGAPTAEIPENPPAVEAPGEEYPEEDKED
                     TDNLNEDKELSDEEIIASIDLSLNPNELGEIMILMYHGIGPEESVWQRTPDNFRKDLE
                     YMYQNGYRMISLNDYAKGEIKTEAGYTPIILTFDDGRQNNFSYIEENGKIVIDPDCAI
                     GILEEFKNKYPDFNVTASFFLGTNLFGQKEYAEKKLNWLIENGYDLGNHTYSHNEMAE
                     LNSDEIIIEIGTVNNIIKQYLPDYAVETLALPHGSNPKDEFIESVLEGEYEGNKYRTL
                     AVLDVGWRPAYSPFDILTDFSNLYRVTASEINVDNCGINDYFKQYEENKRERFISDGN
                     PDVVTIPKRHVDYLNMDIVGDRIVNIYE"
     gene            2135..2839
                     /locus_tag="PLD67_06385"
     CDS             2135..2839
                     /locus_tag="PLD67_06385"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008401036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HOT22182.1"
                     /translation="MIAELKEIISKSDNIVFFGGAGVSTESGIPDFRSVDGLYSKKYK
                     YPPEMMVSHSFFRSNTEEFFDFYINKMIFLNAKPNSAHKKLAELEKEGKVKAVITQNI
                     DGLHQAAGSKNVLELHGSVHRNYCMKCRKFYDVNFIVNSKGIPKCQCGGIVKPDVVLY
                     EESLNTDVIEKAVNFIERADVLIVGGTSLVVYPAAGLINYFKGSKLVLINKSSTPMDR
                     RADLVINDSIGKVFED"
     regulatory      2896..3012
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
BASE COUNT         1104 a          477 c          571 g          942 t
ORIGIN      
        1 aatatttatg ttaccaattc caaaaagcag caaattgatg taatagaaaa acaagggcat
       61 aatataagca ccttaaaact aatgtttatc cccggtttaa tagaaattat accttaattt
      121 gttaatgcat taataatttg tccgaatatt ttctcaggat tggtttccca tcttttaatt
      181 attttttttg ccataatttc atcagtcaca ttaatgctta aattaaaaat agttgtggag
      241 ttttctgaaa cttgaagaga tactataaat tcatcatctt ttcttttata ataatggccg
      301 aatatttctc tctgcttttt tatatgtttt ttaatacctg aataatttct tgctacttca
      361 ttaataaaat attcggggag tttatctccg aacatttcta aggcagcctt gcctgacttt
      421 gagacggagt aatactcact tccattataa ggcttaaggt gtataaactt aacttcacaa
      481 agatcactta acaaaagctg caatgtgaaa taatccatca tgctatattc caatatataa
      541 ttagtaattt cattatttgt caaaggtata tcaatataat ccaatatata caaaagttta
      601 agcttatctt gggtactttt attgctgctt atcaattttg ccctccatta atcatataat
      661 tattccgggg acgaagactg tcatccccct aagtacaggg acattccttc tacaagcaaa
      721 gactttccct aaaataagaa ggtgtgtccc tcttcatctt ttcctattat atcaaacatg
      781 cagttaataa taaactaaaa tttgaaaata cttcatgtag acatttatac ttaaataata
      841 tagtataata ataaggaaag aggaggaaaa atgaaaaaaa taattatttt aatagcatta
      901 ataatgatga caggatgtgg agctcccaca gcagaaattc ctgaaaaccc ccctgccgtc
      961 gaagctccgg gtgaagaata ccctgaagaa gataaagaag ataccgataa tttaaatgaa
     1021 gacaaagagc taagcgacga agaaataatt gcttccatcg atttgtcgtt gaaccctaat
     1081 gagcttgggg aaattatgat attgatgtat catggaatag gtccggaaga atcagtatgg
     1141 caaagaactc cggataactt tcgcaaggac ttggagtaca tgtatcaaaa cggttaccga
     1201 atgataagtt taaatgatta tgctaaggga gaaataaaaa ctgaagcagg atatactcca
     1261 ataatactta cctttgacga cggaaggcaa aataatttca gttatattga agaaaacggg
     1321 aaaattgtaa tagatcctga ctgtgccatc gggattttgg aagaatttaa aaataaatat
     1381 cccgacttta atgtaactgc cagctttttt ttgggaacta atttgttcgg acaaaaggaa
     1441 tatgcagaaa aaaaacttaa ttggcttatt gaaaacggat atgatttggg aaaccacacc
     1501 tacagccata atgagatggc agaattaaat tcggatgaaa taataattga aataggcacc
     1561 gtaaacaaca taataaaaca atatttgccc gattatgcag tagaaacatt ggctcttcct
     1621 cacggcagca atccaaaaga tgaatttata gaatcggtgc ttgaaggaga atatgaaggc
     1681 aataaatata ggacccttgc agttctggac gtaggctgga gacctgccta ttcacctttt
     1741 gatatattga cagacttcag taacctctac cgtgtaactg caagtgaaat taatgtggat
     1801 aattgcggga ttaacgatta ttttaaacaa tatgaggaaa acaaaagaga aagatttata
     1861 agcgatggca atcctgatgt tgtaaccatt ccaaaaagac atgtcgacta ccttaacatg
     1921 gatattgtag gggacagaat agtaaatatt tacgaataat gacaagggga gctctgctcc
     1981 tcaggttatt ggcatactcc attatgtcat cctgagcgta agcgaaggat ctcttcttat
     2041 agggagctct gctccttttt tattgcgata ggtttgagag aaataaggaa atatagtata
     2101 atggttaaaa gaataataag agggagatgt tgttatgata gctgaattaa aagaaataat
     2161 atcaaaaagt gataatatag tattttttgg aggagcaggt gtttcgaccg aaagtggtat
     2221 accggatttc aggagtgttg acggattgta cagcaaaaaa tataaatatc cgcctgaaat
     2281 gatggtttcc cacagtttct tcaggagtaa taccgaggag ttctttgatt tttatataaa
     2341 taaaatgatt tttttaaatg ctaagccaaa cagtgcccat aaaaagcttg ctgaacttga
     2401 aaaagagggg aaagtaaaag ctgttattac acaaaatatc gatggacttc atcaagccgc
     2461 aggaagcaag aatgtattgg aactgcatgg ctcggtacat agaaattact gcatgaagtg
     2521 cagaaaattt tatgatgtga attttattgt caactcaaaa ggaataccta aatgccaatg
     2581 cggaggaata gttaagccgg atgttgtatt gtatgaagaa agtcttaata ccgatgttat
     2641 agaaaaagcg gttaacttca tagaaagggc agatgtttta attgtcggcg ggacatccct
     2701 tgtagtttat cccgctgccg gccttattaa ttactttaag ggaagcaaat tggtactcat
     2761 taacaaatca tcaactccaa tggatagaag agccgattta gttatcaatg acagcatcgg
     2821 taaagtcttt gaagattaat tatcaattaa tgtatttaca ctccattcac attgtgatat
     2881 aattaaaact aataaaaagc ttaatagctc cgagcataac agcctaagaa tgatatttat
     2941 ggccttatgc cgatagggtt tgattggaaa cggtcaagcc tcctggtgga aaggagaaac
     3001 tagataatat gtgaacagca attccatcag aagtgctgtt ttttagttcg tttacttttc
     3061 tgatttatat ttagtctaac taaattaaaa taaa
//
LOCUS       DAOSOW010000225         3084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_31703_length_3084_cov_3.105315, whole genome shotgun sequence.
ACCESSION   DAOSOW010000225 DAOSOW010000000
VERSION     DAOSOW010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31703_length_3084_cov_3.105315"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..434
                     /locus_tag="PLD67_06390"
     CDS             132..434
                     /locus_tag="PLD67_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22183.1"
                     /translation="MNMIKNYNKYIKEADFYYNEKDKTIFRLLRFLQFENIESYFIKM
                     ESSLLEGKLYEIVLKDFEYAVLHSKSLWLLDVLKKEKVFTEKRKKSVIDYYVNQRY"
     gene            complement(571..1032)
                     /locus_tag="PLD67_06395"
     CDS             complement(571..1032)
                     /locus_tag="PLD67_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22184.1"
                     /translation="MKRIINAIRDFIYNITDYGLIITVIVVMTVILVWRFDLLFSRGI
                     EKEIIKDLPIINEPEVNEPIAEEPDPEDEEPEPEDETPTGLIAAISIPQGSFPSQIAE
                     ILLNNNLIKSKDEFLTRCVELDLDTRLQSGTFNIEVGTPLDDVIKIIANAY"
     gene            1234..2142
                     /locus_tag="PLD67_06400"
     CDS             1234..2142
                     /locus_tag="PLD67_06400"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOT22185.1"
                     /translation="MKEIKITKNEEGQRLDRFLKKYLNKANQSFIHKMIRKKNIKVNG
                     LKAGPDIVLKTDDTVQLYLSDETIDKFRDKKTLQKTDIHFDIVYEDDNILVVNKPIGL
                     STQPDKTGKKNLVDEIKVYLDAKEENISFTFKPAVCNRLDKNTSGLVIAAKNYDALKQ
                     TNKAIRERKIRKYYMAKVHGIIKDDLELKDYLIKKDNKNMVKIINEYRENSKSVITYA
                     HPLKREGKYTWLEVEIETGRAHQIRAHLASIGHPIAGDKKYGKKDNEKYQVLHAYKLL
                     LDGFEGNLSYLNGMEIVSDISERKIL"
     gene            2153..>3084
                     /locus_tag="PLD67_06405"
     CDS             2153..>3084
                     /locus_tag="PLD67_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOT22186.1"
                     /translation="MKIKLANTDEVIEVYENETLYRILKRVYKNDYFKYLGAKVNNKL
                     KGLCSKDFKENDRIEFIDITNPDGHRIYVRTLSLIYVKACKEIYNDIDVSINHSLNKG
                     LYTELQYKHLVTKKHLDAIKNKMQEIIDAKKNITTYFLSVDKAREIFSAQGMMDKVEM
                     LKYWNKDTIRVYELDGYYDTFYGYVAPNTGFVNKFDLRLFYPGIILNYPTREYNFELP
                     EYIEQKKLSKVFKEAEDWGEIMDVAYVGALNKKIVNNEIYDMIRINEALHEKKIAYIA
                     DEITNDKNIKIVLIAGPSSSGKTTLAQRLSIQFRV"
BASE COUNT         1207 a          427 c          526 g          924 t
ORIGIN      
        1 gatttagatg atgaacaatt agaatatgta aatttaataa aaaaatcttc aaaattttta
       61 atattattca ttaatgtata attgcaattt atcttatata taaaaaaata atagtgaaat
      121 tttatatata aatgaatatg ataaaaaatt ataataaata tataaaagaa gctgattttt
      181 attacaatga aaaggataag acaattttcc gcctgttaag gtttcttcaa tttgaaaaca
      241 tagaatcata ttttattaaa atggaatcat cattattaga aggcaagctt tacgaaatag
      301 tattgaagga ttttgaatat gctgtacttc attctaagag cttgtggctt ttggatgttt
      361 taaaaaaaga aaaagtattt acagaaaaga ggaagaaatc agttattgat tattatgtaa
      421 atcaacggta ttaaggtgtg gaaacacacc ttcttttatt gtccaacggg gacggttctt
      481 tctggacaaa taaattgtcc agaaagaacc gtccccgttg gataagaacc gggaaggttc
      541 tttacggaaa gaaccttccc aatctgatat ttaataagca ttcgcaatta ttttgataac
      601 atcatcaaga ggtgttccta cttcaatatt aaatgttccg ctctgcagtc ttgtatcaag
      661 gtcaagctca acacagcgag taagaaactc atctttgctt tttattaaat tgttgtttag
      721 cagaatttcc gctatttgtg aagggaatga gccttgagga atactaatgg cagcaattaa
      781 ccctgtcggt gtttcatctt ccggctccgg ctcttcatct tccgggtccg gttcttccgc
      841 aatcggttca tttacttcgg gttcatttat tataggcagg tctttaatta tttctttttc
      901 aattcctctg ctgaacaaca aatcaaatct ccaaaccagt ataactgtca taacaacaat
      961 tacggttata ataagtccgt aatcagttat attatatata aagtctctta ttgcattaat
     1021 tattcttttc atttgtcacc tcttagctta tcgctcttat tatattaaca caaaaatgag
     1081 atgcatacaa tatccacatt taataaataa ttgtaaaaac tgtaaaatgc atatactttc
     1141 ataaaatatt tgttataata gtatagagaa acatagtgcc cgtaccctcc ggacaaatat
     1201 ttaatttacc taagaaaaag taaaggataa cacatgaaag aaattaaaat taccaaaaat
     1261 gaagaaggtc aaagactgga caggttccta aagaaatatt taaataaggc aaatcagtca
     1321 tttatacata aaatgattag gaaaaagaat ataaaggtaa acggcttaaa agcaggaccg
     1381 gatattgttt taaaaacaga tgacacagtc cagctgtatt tgtctgatga aacaatagat
     1441 aaatttagag ataagaaaac acttcaaaag accgatatac attttgatat tgtatacgag
     1501 gatgataata tattagtagt aaacaaaccc ataggcctaa gcactcagcc tgataaaaca
     1561 ggaaagaaaa atttagttga tgagataaaa gtatatttag atgcaaaaga agaaaatatc
     1621 agttttacat ttaagcctgc cgtgtgcaac cgcttggata aaaatacctc aggccttgta
     1681 atagcggcaa aaaactatga tgccctgaaa cagacaaaca aggcaataag agaaagaaaa
     1741 atcagaaaat actacatggc aaaggtacac ggaatcatta aggatgattt ggagcttaag
     1801 gattatctta ttaaaaaaga caataaaaat atggttaaaa ttataaatga gtacagagaa
     1861 aattcaaaat cagtcataac ctatgcacat cccttgaaaa gagaaggaaa atatacatgg
     1921 ctggaagtag aaattgaaac aggcagagca catcaaattc gggctcattt agcttcaatc
     1981 ggacatccca tagcaggaga caaaaaatac gggaaaaaag acaatgaaaa atatcaggta
     2041 ctgcatgcgt ataagttgtt gcttgatgga tttgaaggca atttatcata tttaaacgga
     2101 atggaaattg tatcggatat cagtgaaaga aaaatattgt aggaggttag gaatgaaaat
     2161 taaattagca aatactgacg aagtaattga agtatacgaa aatgaaacat tataccggat
     2221 acttaagaga gtttataaaa atgattattt taaatatttg ggtgctaagg taaacaataa
     2281 gcttaaggga ctttgcagca aagattttaa ggaaaacgac aggatagaat ttattgatat
     2341 tacaaatccc gatggtcaca gaatttatgt tcgcaccttg tcattaattt atgttaaagc
     2401 atgcaaggaa atatataacg atatagatgt ttccattaat cattctttaa ataaaggact
     2461 ttatacggaa cttcaatata aacatttagt aacaaaaaaa cacctcgatg ccataaaaaa
     2521 taaaatgcag gaaattattg atgctaaaaa aaatattaca acttactttt taagtgttga
     2581 caaggcaaga gaaatatttt cggctcaggg tatgatggat aaggttgaaa tgcttaagta
     2641 ttggaataag gatacaataa gagtgtatga attggacgga tattatgata ccttttacgg
     2701 atatgtcgct ccaaatacag gatttgtaaa taagtttgat ttaagattat tttatcccgg
     2761 aataatacta aattatccca cccgtgaata caattttgaa cttcccgaat atattgagca
     2821 aaagaaactt tcaaaagtat ttaaggaagc tgaggactgg ggggaaataa tggatgttgc
     2881 atatgtcggc gctttgaaca aaaaaatcgt caataatgaa atatatgata tgataaggat
     2941 taacgaagct cttcacgaaa aaaagattgc atatattgcc gatgaaatta ctaatgataa
     3001 aaatataaaa atagtcctaa ttgcaggtcc ttcttcttca ggtaaaacaa cattggcaca
     3061 aagattgtcg attcaatttc gagt
//
LOCUS       DAOSOW010000226         3075 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_31813_length_3075_cov_3.964238, whole genome shotgun sequence.
ACCESSION   DAOSOW010000226 DAOSOW010000000
VERSION     DAOSOW010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31813_length_3075_cov_3.964238"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1960)
                     /locus_tag="PLD67_06410"
     CDS             complement(<1..1960)
                     /locus_tag="PLD67_06410"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOT22187.1"
                     /translation="MLFNKIFTDESLDKIKIQKIEVIERTNTINLHALSQEIIDFNHI
                     ENIKDEISKHYNNSVNVEIYLKYKLEKNINIEKIKENLLYIINKSSNILSKAKDNIKI
                     EFKDECFFVIADNKLLIDELNDNNTERKLKKLICDYNLDYNIKAIYNEAEEEAAITAQ
                     RIFDQALKKNLEIIKNNSSEKKPANKSQEGSYKKRSRKKIDFDSIPVSLISELTNDSG
                     VVNIKGKIVNVDKRELKNGSFLISLIITDSTDSIMCKYFCPNGFDDEGIENGNYIQIA
                     GNVEYDDYEKELIVKIQDLQLTTEEIKRMDNAKEKRVELHLHTGMSSMDGINSFKEYA
                     KKARQWGHKAIAITDHGVVQGYPEAMDCADDNFKVIYGMEGYLVNDTVSIVQNCQNVN
                     LDSVFVVFDIETTGLNPKNDNIIEIGAVKISNRKVIDSFSTFVHIDKKLPGKIIELTS
                     ITDDMLQGQPEIDEALPAFLDFAKECVLVAHNAKFDLGFIKENTKVLSIENYNPPVLD
                     TLELSKALIKDVKNHRLNTLTKKLGINLINHHRAVDDANATGQLLIILLNKLSEREIY
                     DVSKLNDILMEDIDIVKQPSYHINILVTNYIGLKNLYSLVSDSHINNFYKKPRLLKSN
                     LNQLREGLLLGTACESGELFSAFLEGKSD"
     gene            2162..2782
                     /locus_tag="PLD67_06415"
     CDS             2162..2782
                     /locus_tag="PLD67_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HOT22188.1"
                     /translation="MICGGIIGLERSRKNRPAGFRTYMIVCLSSALVMMTNQYIYNNF
                     SGSDPARLGAQVISGIGFLGAGTIMITSRNQIKGLTSAAGLWASACLGLAIGVGFYSG
                     AVIVALIVFIIITFFKKIDYMLTSNNRIINIYTSFKSIEQLDKFIIFCNLSDFKVRNI
                     EITKDTIPHEISVIAIITLEAKERINHVDVIQKLNSFEGLVHIEEL"
     gene            complement(2834..>3075)
                     /locus_tag="PLD67_06420"
     CDS             complement(2834..>3075)
                     /locus_tag="PLD67_06420"
                     /inference="COORDINATES: protein motif:HMM:NF026044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22189.1"
                     /translation="TQLDSITVKGLPQNIPDDLTIDVSNIEKVENICLKDVKFTEGIV
                     PEGHPEQVLISIVEAKRTVEEEETGEETDDEVAGE"
BASE COUNT          943 a          513 c          433 g         1186 t
ORIGIN      
        1 catcactctt gccttcaaga aaggcactga aaagttctcc tgattcacaa gcagttccga
       61 gcaatagacc ttctcttaat tggtttaagt tagattttaa cagtcttggt ttcttgtaaa
      121 aattatttat atgtgaatct gaaaccaaac tgtataaatt tttaagtcct atgtagttgg
      181 taacaagtat attgatatga tatgacggct gtttaacgat atcgatgtct tccataagga
      241 tatcatttaa tttagaaaca tcatatattt ctctttcact taacttgttc aataaaatta
      301 tcaaaagctg tcccgttgca tttgcatcat caactgctct gtggtggttt ataagattta
      361 tgcctaattt tttcgttaaa gtattaagtc tatggttttt aacatccttt atcagagctt
      421 ttgagagctc caaagtatcc aagaccggag gattataatt ttctatagac agtaccttag
      481 tgttttcttt aataaaacca aggtcaaatt tagcattgtg agcaaccaat acacattctt
      541 ttgcaaaatc caaaaatgcc ggcaaagcct catctatttc gggttgacct tgaagcatat
      601 catctgttat tgatgttaat tcaatgattt ttccaggaag ctttttatca atgtgtacaa
      661 atgttgaaaa gctgtcgata acctttctgt ttgaaatttt cactgctccg atttctatta
      721 tgttgtcgtt ttttggattt aaacctgtag tttctatatc aaatacaaca aatacagaat
      781 ccaaattaac attttgacaa ttttgaacta tagagacagt gtcatttaca aggtatcctt
      841 ccataccgta tatgacttta aaattatcat cggcacaatc catcgcttca ggatagcctt
      901 gcacaacacc gtggtctgtt attgcaattg ccttatgacc ccactgcctt gccttcttag
      961 cgtattcctt aaagctgttt ataccatcca ttgagctcat acctgtatgc agatgaagct
     1021 caaccctctt ttcttttgca ttatccattc gcttgatttc ttcggttgtt aactgtaaat
     1081 cttgtatttt aacaatcaat tctttttcat aatcatcata ttcaacattg cctgcaattt
     1141 gaatataatt cccattttca attccttcat catcaaaccc attaggacaa aaatatttgc
     1201 acattattga atccgtggaa tcggttatga ttaaggatat gaggaaagag ccgtttttta
     1261 actccctttt gtcaacattt acaatttttc ctttaatatt aactacacct gagtcatttg
     1321 ttaattcgga aattaaagac acaggtatgc tgtcaaagtc aatctttttt cttgaccttt
     1381 tcttataact gccttcttga gatttgttgg caggtttttt ttcggaagaa ttatttttaa
     1441 ttatttctaa attctttttc aatgcttggt caaaaattct ttgtgcagta attgctgctt
     1501 cttcttccgc ctcattatat atagctttta tattatagtc taaattgtaa tcgcatataa
     1561 gtttcttaag ctttctttct gtgttattgt cattaagctc gtctatcagc agtttattgt
     1621 ctgcaattac aaaaaaacat tcatctttaa attcaatttt tatattgtcc tttgcttttg
     1681 acagtatatt tgaactttta ttgatgatgt aaagcaggtt ttctttaatt ttttcaatgt
     1741 ttatattttt ttctaattta tattttaaat atatttctac atttactgaa ttgttataat
     1801 gcttacttat ttcgtcctta atattttcta tatgattaaa atctataatt tcttggctta
     1861 acgcgtgtaa attaatagta tttgttcttt ctattacttc aatcttttgg atttttatct
     1921 tatcaaggct ctcatcagta aatattttat taaataacat agcagtctcc ctttattttc
     1981 tcctattgta gtataacaaa attaaaattt taattcaata tacttaaaat atattttaaa
     2041 agtaaacata tgtaataaat tttacattta ttaatatatt aataaataca aaggaggaat
     2101 tccaatcgaa acgctatatg aactcaatat atattccata acattgcgaa ttgtactttc
     2161 tatgatttgc ggaggtatta taggacttga aagaagcagg aaaaacagac ctgcaggttt
     2221 cagaacttat atgattgtat gtttaagctc tgctttggta atgatgacaa atcaatatat
     2281 ttacaataat ttcagcggca gtgaccctgc tagacttggt gcacaggtta tcagcggtat
     2341 cggttttttg ggtgcaggaa caataatgat aacaagcaga aaccaaataa aaggacttac
     2401 ttcagctgca ggtttatggg catctgcatg cttaggcctt gcaataggtg tagggtttta
     2461 ttcaggtgca gtaattgttg cattgattgt ttttattata ataacctttt ttaaaaaaat
     2521 tgattatatg ctaacatcaa acaatagaat tatcaatata tacacttcgt ttaaatccat
     2581 cgagcaattg gataaattca taattttttg caatttatct gattttaaag tacgcaacat
     2641 tgaaattaca aaagacacta taccccacga aatatcggtt attgcaataa ttacactgga
     2701 agcaaaagaa agaataaatc acgttgatgt aatacaaaag ctaaactcct ttgaaggact
     2761 tgttcatata gaagaattgt aaaaaaaata cccgtaaatt gggtattttt atttaatact
     2821 attattaatc ttgttattca cctgctactt catcatcggt ttcttcgccg gtttcttctt
     2881 cctctactgt tctcttagct tcaacaatag agattaaaac ttgttcaggg tgaccttcag
     2941 gaactattcc ttcagtaaat tttacatctt ttaaacatat attttctact ttttcgatat
     3001 ttgaaacatc aattgttagg tcatcaggga tattctgcgg caaacctttc actgtgattg
     3061 aatctagttg tgtta
//
LOCUS       DAOSOW010000227         3064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_31966_length_3064_cov_3.593220, whole genome shotgun sequence.
ACCESSION   DAOSOW010000227 DAOSOW010000000
VERSION     DAOSOW010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31966_length_3064_cov_3.593220"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..594
                     /locus_tag="PLD67_06425"
     CDS             106..594
                     /locus_tag="PLD67_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015045139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT22190.1"
                     /translation="MLYLDNRFMEVALPIIEHEEYQQMRYIKHHDESVFEHSLKVAFY
                     SYQLAYKQNLDWKSTIRGALLHDFFLYKFKKCFSLRIITDSIKHAIIHPKIAFDNAVK
                     FFDLNKKEENIIKGHMFPFGFPKSKEAWIVSFVDKYIAVFEYSSNFKKMMHKKQPAYQ
                     AE"
     gene            806..1336
                     /gene="raiA"
                     /locus_tag="PLD67_06430"
     CDS             806..1336
                     /gene="raiA"
                     /locus_tag="PLD67_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOT22191.1"
                     /translation="MKISIYSKNLQLTDALREASIKKLRRLDKFFQQDIEAKVVLSIE
                     KKIHKVEVTIPFNGRIVRVEEYSDDMYNALDEAVESLEQQIRKHKTKLQNKRHSSQSI
                     KFENIEPLDYEEEEEFKVVKTKRFAIKPMNIEEAILQMDLLKHNFFVFLNADTEEVNV
                     VYKRKDGNYGLIEPEL"
     gene            1972..2355
                     /locus_tag="PLD67_06435"
     CDS             1972..2355
                     /locus_tag="PLD67_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22192.1"
                     /translation="MLETILRIAVPILTLIFGTLSAYFRARDKLRDSSIKYIAEAEEL
                     YKDNTKAGKEKFSWVVEALYDLIPAPLKMFITKKCIEKIVQSSFDAIEDYAKMQFEIA
                     IEKYLKHLSDGKSGDFIDFIKSDGE"
     gene            2352..2945
                     /locus_tag="PLD67_06440"
     CDS             2352..2945
                     /locus_tag="PLD67_06440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT22193.1"
                     /translation="MIQLKHLPLDDISISSPYGARSITVNGRYYWWHNGLDLKAQLNT
                     PVYAVADGEVMAARYDNSYGYYIAIDHGKFGTLYAHLSHYKVTEGSIVKSGEIIGHTG
                     NTGDTTGPHLHFEIRLGSYANFWERAHCDRSVFMNTIDPMLFIEDFFNKKDNLSVEEA
                     AKIVQSAAGLEDKTMDYIVKHYRFGDDLVKKLAKAIQ"
BASE COUNT         1138 a          466 c          513 g          947 t
ORIGIN      
        1 tgcatgtttt attaaaaatt tttattgcaa ttttttgagt ttcattataa gatgtaaaaa
       61 gcattatgca atgcgcaacc cttacaacta ataaaggatg gacggatgtt atatttagat
      121 aatagattta tggaagtagc acttccaatt attgagcatg aagaatatca gcaaatgaga
      181 tatattaagc accatgatga aagtgtattt gagcattcat taaaagtagc attttattca
      241 tatcaattgg catataaaca aaatttagac tggaaatcaa caataagagg agctttactc
      301 cacgactttt tcctgtataa atttaaaaaa tgttttagtt taagaataat aacagattca
      361 ataaaacatg caataattca tcccaaaatt gcttttgaca atgcagttaa attttttgat
      421 ttaaacaaaa aagaagaaaa tatcataaag ggacatatgt ttccttttgg ttttccgaaa
      481 tcaaaagaag catggattgt aagctttgtg gataagtata ttgctgtatt tgaatactct
      541 tctaatttta agaaaatgat gcataagaaa caaccggcat accaagccga atagtattat
      601 ggctataaat aattccatag tgtcattctg agggcattgc ccgaagaatc tcataagagc
      661 cttcgcttac gctcaggatg acagtatata attagacacc ccaacggatg tctttttttt
      721 attttgacaa atattttgtt tatttttctc ataaatgggt aaaatttaaa tatatataaa
      781 taaatacttg aaaggggctg actaaatgaa aatatctatc tacagcaaaa atttgcaact
      841 taccgatgca ttaagagagg catcaattaa aaagctaagg aggttagata aattttttca
      901 acaagacatt gaggcaaaag ttgttttaag tattgaaaag aaaatacata aagtagaggt
      961 cacaattccc ttcaatggaa gaattgtacg tgttgaagaa tattcggacg acatgtacaa
     1021 tgcattagat gaagcagttg aatctttaga gcagcaaata agaaaacata aaacgaaact
     1081 acagaataaa aggcactcaa gtcaatcaat caagtttgaa aacatcgagc ccttagacta
     1141 cgaggaagaa gaagaattta aggtagtaaa aaccaagcgc tttgcaatta agcccatgaa
     1201 catagaggaa gcaatactgc aaatggattt attaaaacac aatttttttg ttttcctgaa
     1261 tgcagatacc gaagaagtaa acgtagtata taaaaggaaa gatggaaatt acggtttaat
     1321 tgagccggaa ttgtaaaacc taactttaaa tatagatatc aaaaagacgc agcacgcgtc
     1381 tttttttact tttacctgca tccttaacgt accataataa tactaggaca cttaatgtat
     1441 tttagaaata cgtttgcatg attgtcgaat aatgtataaa tttagcgctt atgctttact
     1501 tataattata ccttgttaat aatttaagtg tataaatcaa gttgtccaca ggcgtggata
     1561 aattgtttaa tttctaataa tgcttaatat tgaaattcca acaaaaaata tttttgtttt
     1621 tttgtaaaaa aacacttgac aaatatttcc cttaaagtcc ccgaatttaa gaattgttaa
     1681 caatttaatc cacagctgtg gataagttga aaacatatag aacatatctt ctattttttt
     1741 gagattgtta aatatattta accggtttta atcttattta attagttgaa catccaatgg
     1801 aaaagtctgg aatgttaatt ttctattttt ttgaataatt aatagttaac tttacttgta
     1861 attttataaa actaataatt attatacaca tttttcacaa ttgccttaat tacaattcat
     1921 taagaacaaa aatttatctt aatgaatata atgccttagg gatgtgataa catgttagaa
     1981 acaatactaa gaattgctgt tccgatttta accttaatct ttggaacatt gtcagcttat
     2041 tttagagctc gtgacaaact cagagacagc tctattaaat atattgcaga ggcagaagaa
     2101 ttatacaaag ataacacaaa agcaggtaaa gaaaaatttt cttgggttgt cgaagcctta
     2161 tatgacttaa tacctgcccc tttaaaaatg tttataacaa aaaaatgtat tgaaaaaatt
     2221 gttcaatcct cctttgacgc aatcgaggat tatgcaaaaa tgcaatttga aatagcaatt
     2281 gaaaaatacc ttaaacatct tagcgatgga aaatcaggag acttcattga ttttataaag
     2341 agtgatggtg aatgattcaa ttaaaacacc tgcccttaga cgatatttca ataagcagcc
     2401 cctacggagc cagaagtata actgttaacg gcagatacta ttggtggcac aacggattgg
     2461 atttaaaagc acagcttaat actcccgtat atgcagtagc agatggagag gtgatggctg
     2521 caagatacga taacagctac ggctactata tagccataga ccacggcaaa tttggcacct
     2581 tgtatgctca tctttcacat tacaaagtta ctgaaggaag tattgtaaaa tcaggtgaaa
     2641 taatcggcca taccggcaac accggcgata ctacaggtcc ccacctgcat tttgaaataa
     2701 gacttggaag ttacgcaaac ttctgggaga gagcacactg tgacagaagt gtttttatga
     2761 acactataga ccccatgctt tttatagaag acttttttaa taaaaaagat aatttaagcg
     2821 tagaagaagc tgcaaagatt gtccagtccg ctgccggatt agaggataaa actatggact
     2881 atattgtaaa gcattacaga ttcggagacg acttagtaaa gaagctggca aaagctatac
     2941 aatagcaaaa aaaggcggtg ccaggcacct ttattggtgc tgacaccacg tatgtggcac
     3001 cgcctaaaac ttatcgaagt atattttatc cgcaggcaca ttctttttca ccaatgcttc
     3061 aact
//
LOCUS       DAOSOW010000228         3049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_32186_length_3049_cov_2.555110, whole genome shotgun sequence.
ACCESSION   DAOSOW010000228 DAOSOW010000000
VERSION     DAOSOW010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32186_length_3049_cov_2.555110"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="PLD67_06445"
     CDS             complement(<1..606)
                     /locus_tag="PLD67_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HOT22194.1"
                     /translation="MSENCNESCSSCSKECDSRKEEQTNFLERPHPQSNIKKVIGVIS
                     GKGGVGKSLVTSLLAVTMKRRNHNVAILDADITGPSIPKLFGITEKARGGSNGVLPVK
                     SKTGIEVMSINLLLENTTDPVVWRGPIIAGTVKQFWTDVIWKDIDFMFIDMPPGTGDV
                     PLTVFQSIPVDGIIVLTSPQELVSMIVEKAVKMAESMNIPII"
     gene            complement(616..1467)
                     /locus_tag="PLD67_06450"
     CDS             complement(616..1467)
                     /locus_tag="PLD67_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT22195.1"
                     /translation="MKQLLILSGKGGTGKTTVAGAFIKLSNVKAYADCDIDAPNLHLI
                     MQQESIPENSDYYGLDKAVIDKDKCLMCNLCYDSCRFDAINDYTVSLYDCEGCGVCEA
                     VCPASAINLMPYKAGDMTLYRGNQVFSTAELKMGSGNSGMLVTEVKKNMRFAAADADF
                     AIIDGSPGIGCPVIASISGVTMVLVVAEPSLSGISDMKRIIETSKKFNTKLAVCVNKY
                     DTNIENNETIKQFCQGNKIDFVGEIPFDKKAVEAVNNGLTIVDIDCAAGEAVRKIYKK
                     TMKILFN"
     gene            complement(1464..2297)
                     /locus_tag="PLD67_06455"
     CDS             complement(1464..2297)
                     /locus_tag="PLD67_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT22196.1"
                     /translation="MKIAVLSGKGGTGKTFVSVNLSFAVDNSNYIDCDVEEPNGHLFF
                     KPNIIKSEKVHVKIPNINNELCNGCRRCVDFCKFNALAYIKDKVYVFDEICHSCGGCM
                     LLCPEKAISEKDKEIGEIQEGVFNNSNIYTGILNPGEETGVSIIKKLLKITDNKPYTF
                     IDCPPGSSCSVMESIKDVDYCILVAEPTLFGVHNFNMVYNLVKLFDKPYGVVLNKCLD
                     GENPAEKYCNEKNIKVLCKIPFEHELGKLNSNAEIASEKNEKYKSLFSSLLKTIKEEV
                     K"
     gene            complement(2297..2650)
                     /locus_tag="PLD67_06460"
     CDS             complement(2297..2650)
                     /locus_tag="PLD67_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOT22197.1"
                     /translation="MKIAIPVDDGNVCVSFGRTPYFMLYDTENNDSSFINNSAANSQG
                     GAGIKAAQIIVDNDADILITPRCGQNAAEVLTAANVKIYKALNDSIEENLTAFKNDKL
                     KILTEIHSGFHGGEK"
     gene            complement(2719..3039)
                     /locus_tag="PLD67_06465"
     CDS             complement(2719..3039)
                     /locus_tag="PLD67_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOT22198.1"
                     /translation="MYDYLGRAPYFLIYSTVTKEYEFLDNRAVVEQGGAGIRAAQVIA
                     DNGVKAVITNRCGENAEKLFRNAEVFVYAAQKGTTEENIVYFNENKLSLLNEFHSSFH
                     GKEK"
BASE COUNT          861 a          654 c          441 g         1093 t
ORIGIN      
        1 aatgattgga atattcatcg attcagccat tttcaccgct ttctccacaa tcattgacac
       61 caattcctga ggagatgtta atacaatgat tccatcaacc ggtatggatt gaaatacagt
      121 aagtggcaca tcacctgttc ccgggggcat gtcaatgaac ataaaatcta tgtctttcca
      181 aattacatct gtccaaaact gttttaccgt tcctgctatt atggggcctc tccatacaac
      241 agggtcggta gtgttttcca acagaagatt aattgacatt acttctatgc cggtcttact
      301 tttcacaggc agtaccccat tggaaccacc ccttgctttt tcggttatac caaataattt
      361 tggtattgaa ggccctgtaa tatcagcatc caatattgca acattatgat ttcttctctt
      421 catggtaact gccagcaagg atgttaccaa tgatttgcct actccaccct taccgcttat
      481 aacaccaatc acttttttga tgttgctttg agggtgcggc ctttccaaaa aattagtttg
      541 ttcttctttt ctgctgtcgc attccttact gcaggaacta cagctttcat tacaattttc
      601 actcattttc ttctcctaat tgaataaaat tttcattgtc tttttatata tttttcgcac
      661 ggcttcacct gccgcacaat caatatcaac aattgttaat ccattattta cagcttccac
      721 ggctttttta tcgaaaggaa tttcccccac aaaatcaatc ttattacctt gacaaaattg
      781 ttttattgtt tcattgtttt caatattggt atcatattta tttacgcaaa cagccaattt
      841 tgtattaaat ttttttgatg tttctataat tcttttcata tcgctgattc ccgacaaaga
      901 aggctcggca acaaccagta ccatagtgac accgcttatt gatgcaataa caggacaccc
      961 tatgcccggc gagccgtcaa ttattgcaaa atcagcatcc gctgctgcaa acctcatgtt
     1021 tttctttact tctgttacaa gcatgcctga attcccgctt cccatcttta attcggcggt
     1081 tgaaaaaact tgattgcctc ggtataatgt catatctcct gctttataag gcatcaaatt
     1141 tatggctgaa gcaggacaaa cagcttcaca gactccgcat ccttcacaat catagagact
     1201 gacagtgtaa tcattgattg catcaaatct gcagctatca taacacaaat tacacattag
     1261 gcatttatcc ttatcaatca ctgctttatc taatccgtaa taatccgaat tctccggtat
     1321 ggattcttgt tgcataatca aatgcagatt aggagcatct atgtcgcagt cagcgtaagc
     1381 ttttacattt gaaagtttga taaatgcacc ggcaacggtt gtcttccctg tgccgccttt
     1441 accgcttaga attaacagct gcttcattta acctcctcct ttattgtttt tagaagagag
     1501 gagaatagac ttttgtattt ttcatttttt tcagatgcaa tttctgcatt cgaatttaat
     1561 ttgcctaatt catgctcgaa aggtattttg cataaaactt taatattttt ttcattacaa
     1621 tatttttccg ccggattttc tccgtctagg catttattta aaactacgcc gtaaggttta
     1681 tcgaataatt taactaaatt ataaaccatg ttgaaattat gtacgccaaa taaggtgggc
     1741 tcagccacca atatgcagta atcaacatcc tttatgcttt ccataacgct gcaggagctt
     1801 cccggaggac agtcgataaa ggtatatggt ttattgtcgg tgatttttaa caatttcttg
     1861 attatgctta ctccggtttc ttcaccggga tttaaaattc cggtataaat atttgaatta
     1921 ttaaaaacgc cctcctgtat ttctcctatt tctttatctt tttccgatat tgctttttcg
     1981 gggcataaca gcatgcatcc tccgcaagaa tgacatatct catcaaatac atatactttg
     2041 tcttttatat atgccaaagc attgaacttg caaaaatcta cgcatcttct gcacccgttg
     2101 caaagctcat tatttatgtt tggaatttta acgtgcacct tttcactttt aataatatta
     2161 ggcttaaaga aaagatgtcc gttaggttct tccacatcac aatcaatgta atttgaatta
     2221 tcgacagcaa aagataaatt cacagataca aatgttttgc ctgttccgcc cttgccgctt
     2281 aaaacagcaa tcttcattat ttctctccac catgaaaacc tgaatgtatt tcagtcagta
     2341 tttttagttt atcattttta aatgcggtta aattttcttc aattgaatca tttaatgctt
     2401 tataaatttt aacattagct gctgttaaca cctctgctgc attttgcccg catcttggag
     2461 ttattaatat atccgcatca ttatctacaa taatctgagc tgctttaata cctgcaccgc
     2521 cttgactgtt tgccgcactg ttattaataa aactactatc attattttct gtatcataaa
     2581 gcataaaata tggtgtcctg ccaaatgata cacatacatt tccatcatct accggaattg
     2641 ctattttcat tatttcctcc atatttatga catatgccaa taataaatta tatatttata
     2701 atcggcatat gtcaatattt atttttcttt gccatgaaag cttgagtgaa attcatttag
     2761 taaagaaagt ttattttcat taaaatacac tatattttcc tctgtagtgc ctttttgggc
     2821 tgcgtaaaca aaaacttcag catttctgaa taatttttct gcattttccc cacaccgatt
     2881 tgtaattaca gcttttacac cattgtccgc aatcacttgt gcagctctaa ttcctgcacc
     2941 tccctgttca actactgccc tgttgtccaa aaattcgtat tcttttgtaa cagtgctgta
     3001 gataaggaaa tacggtgcgc gaccaagata atcatatata tttgattta
//
LOCUS       DAOSOW010000229         3048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_32192_length_3048_cov_3.810224, whole genome shotgun sequence.
ACCESSION   DAOSOW010000229 DAOSOW010000000
VERSION     DAOSOW010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32192_length_3048_cov_3.810224"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLD67_06470"
     CDS             <1..391
                     /locus_tag="PLD67_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22199.1"
                     /translation="KIKENQLRQRLNENWKTANKSYINNEITNKNYNNVEPIKDNKEK
                     LVYRKEELIPNKEKYLNPSGFSYDEIDENFKKIVDTVLDKEWYYKGFRWVKCKRCNRY
                     YVINFCWSFGGLKKEAILGICNNCTVD"
     gene            484..636
                     /locus_tag="PLD67_06475"
     CDS             484..636
                     /locus_tag="PLD67_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22200.1"
                     /translation="MADNFGLKIGIEGEREFKKALSEINQSFKVLGSEMNLVTSQFDK
                     QDKSIC"
     gene            complement(696..1454)
                     /gene="istB"
                     /locus_tag="PLD67_06480"
     CDS             complement(696..1454)
                     /gene="istB"
                     /locus_tag="PLD67_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOT22201.1"
                     /translation="MPVNKLLIETYFKKLKMPQAAKVYVSLAREAADNNLDYEEYLLC
                     VLEQEVQQRESNRIQRSIRQAGFPVIKTFESFDFLAIPSLNKPRVLKLMQGEYIRRKE
                     NVLLIGNSGVGKTHIAIALGYEACRQGMKVKFYTAAGLINELLAAQQEYRLNKLEKQW
                     LAPHLIILDELGYVPFSKTGAELLFQFCSSRYERGSLIITTNLEFPKWTEVLGEEQMT
                     AALLDRLTHNAHILNINGESYRFKQALAKQTNID"
     gene            complement(1454..2938)
                     /gene="istA"
                     /locus_tag="PLD67_06485"
     CDS             complement(1454..2938)
                     /gene="istA"
                     /locus_tag="PLD67_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOT22202.1"
                     /translation="MIKMAQLENIRKMYYMEDLSIREISRRTGIHRDTISKYISLEEP
                     KPPKYKLSKERKHPVLGPYIPMIEKILEDDKSRPRKQRYTGTKIFEILKAEGFTGGYN
                     TVMDYLRKEYKKQREAFLPLEFELGTYAETDWTEAYFYLKGKEAKAHLFVMKLRGSGG
                     FYVRAYPFEKQEAFFDGHIKCFEFMKGVPYKIAYDNLKTAVKRILEGSNREEQEQFIA
                     LRTHYLYEATFCRPAKGSDKGGVENAGKEAVRKFFVPYPEVDSFEELNEYLHNECNKL
                     LENNPKWEAEKAALRPLPATRFDGARYKEAKVNRYSMVQFETNRYSLPTMYVGERVTV
                     KATADEVKILYKGAIIASHRRVYGQYQEEIKLDHYLELLLQKPRALDNTKVYKPNTLA
                     PIYEQYRRSLNARNHKGNREFVRILMLHRDYPPALVTEAVEIAMAYNVYSYDGVLNIL
                     GQLLVSGRPKIVPVSKDKLQNIPEVVVIPPDLSKYSVLMSGGGK"
BASE COUNT          877 a          596 c          547 g         1028 t
ORIGIN      
        1 aaaaataaaa gaaaaccaat tacgacagag attaaatgaa aattggaaaa ctgctaataa
       61 atcatatata aataacgaaa ttactaataa aaattacaat aatgtagaac caataaaaga
      121 caataaagaa aaactggttt atagaaaaga agaactcatt cctaataaag aaaagtattt
      181 aaatccaagt gggtttagtt atgatgaaat agatgagaat tttaaaaaaa tagttgatac
      241 tgttttggac aaggaatggt attataaagg ttttaggtgg gttaaatgca aaagatgtaa
      301 tagatattat gtaataaatt tttgttggtc atttggtgga ctgaaaaaag aagcgatact
      361 aggaatttgt aacaattgta ccgtagattg attaactaaa taaatcatat gataagtatt
      421 gagataagca ggcactctta acaagggtgc cttttttata ctcaaaaata aggaggtgga
      481 agcatggcag acaattttgg acttaagatt ggaattgaag gagaacggga atttaaaaaa
      541 gctctaagtg aaataaacca aagcttcaag gttctaggct ctgaaatgaa ccttgttact
      601 tctcagtttg ataagcagga caaatcaata tgttaatgtc aattaaattt taggccagtt
      661 accggtctaa aattaggcca ccacttcata aaaaatcagt caatatttgt ctgtttagca
      721 agagcctgct tgaacctata gctttcacca ttaatattca gtatatgagc attatgggtc
      781 aaacgatcaa gtaaggcggc tgtcatctgt tcttcaccta acacctccgt ccatttagga
      841 aattctaagt ttgtagttat aataaggctg cctctctcgt atcgtgagga gcaaaactgg
      901 aaaagcaatt cggctccggt tttactgaaa ggcacatagc ctaattcatc aagaattatt
      961 aagtgaggtg ccaaccattg tttttcaagt ttattgaggc ggtattcctg ttgggcggca
     1021 agtaattcat taatcaaacc ggctgctgta tagaatttta ccttcatacc ctgtcgacag
     1081 gcttcatatc caagtgcaat tgctatatga gttttgccta caccggagtt gcctattagc
     1141 aagacatttt cttttcttcg gatatattca ccctgcataa gcttcaatac ccgtggcttg
     1201 ttcagggaag gtatagcaag aaaatcaaag ctttcaaatg ttttgattac cgggaaacct
     1261 gcttgccgaa tacttctctg gatccggttg ctttcccgct gttgtacttc ttgttccaat
     1321 acgcatagca ggtactcttc ataatccagg ttgttgtcag cagcctctct tgctagggat
     1381 acatatactt ttgcagcctg gggcattttt agcttcttaa aataagtttc tatcagtaat
     1441 ttattgaccg gcattacttc ccacctcctg acatgagtac gctatatttg ctaagatcag
     1501 gaggtattac tacaacctca gggatgttct gaagcttgtc cttgcttaca ggaactatct
     1561 tgggacggcc tgagaccaat agctgcccta atatattcaa aacaccatca tagctatata
     1621 cgttatatgc catagctatt tcaacagctt ctgtcactaa cgccggagga taatccctgt
     1681 gcagcataag tatccttaca aactccctgt tccctttatg gtttctggca tttaagctcc
     1741 ggcgatattg ctcataaata ggtgccagag tattgggctt atatactttt gtgttgtcta
     1801 atgcacgagg tttttgcagc agtagttcca aatagtgatc tagttttatt tcttcttggt
     1861 attggccgta tactcttcga tgacttgcta ttattgcccc tttatatagt atttttactt
     1921 catccgcagt agctttaaca gtgactcttt cccctacata cattgtaggt aaagagtatc
     1981 tgttcgtctc aaactgcacc atggaatatc ggtttacctt tgcctcttta tatcttgcac
     2041 catcaaacct tgttgccggt aatggcctta atgcggcttt ttctgcctcc catttcggat
     2101 tgttttccaa taacttatta cattcattat gtaggtattc gttcagctct tcaaaagaat
     2161 caacctcagg atagggtaca aaaaattttc tgacagcctc ttttcctgca ttctctactc
     2221 cgcctttatc gctccctttc gcaggacggc aaaacgtggc ttcatatagg taatgggttc
     2281 gtagtgctat aaactgttcc tgttcttctc tgttgcttcc ttcaagtatt ctctttactg
     2341 ccgtctttag attgtcatat gcaattttgt atggtacacc tttcataaac tcaaagcatt
     2401 tgatatgtcc atcaaaaaag gcttcctgtt tctcaaatgg atatgctctt acataaaaac
     2461 ccccagagcc tcttaatttc attacgaata aatgtgcctt ggcttcctta cccttaagat
     2521 aaaaatatgc ctctgtccag tctgtttcgg cataggttcc taattcgaat tcaagaggca
     2581 ggaaagcttc cctttgcttc ttgtattctt tcctcagata gtccattaca gtattgtacc
     2641 cacctgtgaa cccttctgcc ttaagtattt caaatatttt tgtccctgta taacgctgtt
     2701 ttcgaggtct gctcttatca tcttccagta tcttttcgat cattggtatg tatggaccca
     2761 gtaccggatg tttcctctcc tttgataatt tgtattttgg gggttttggt tcttccaggg
     2821 atatatattt tgagatagta tccctgtgta taccagtcct acggctaatt tccctgatac
     2881 ttaggtcttc catatagtac atttttctga tattctctaa ttgtgccatc ttaatcatac
     2941 ctttcctcct gtacgatata gtattggtaa cttaatcata caggaaattt gattaaggtg
     3001 gcctactttt tcaccggtat gtggtacaat tttagagtag cattaaca
//
LOCUS       DAOSOW010000230         3046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_32230_length_3046_cov_2.848546, whole genome shotgun sequence.
ACCESSION   DAOSOW010000230 DAOSOW010000000
VERSION     DAOSOW010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32230_length_3046_cov_2.848546"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="PLD67_06490"
     CDS             <1..965
                     /locus_tag="PLD67_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOT22203.1"
                     /translation="LGAGAFVCGEETALLQSLEGKRGMSRNKPPFPANKGLWGKPTVI
                     NNVETLANIPQIILRGPEWFKSFGTEKSPGTKVFALGGKINNTGLVEVPMGTTLREVI
                     YDIGGGCPNGKEFKAVQTGGPSGGCLTTKHLDTPIDYDSLTAAGSMMGSGGMIVMDED
                     NCMVDIARFFLDFTVDESCGKCTPCREGTKRMLEILNKITEGKGTMEDLDKLEKLAIN
                     IKETSLCGLGQTAPDPVLSTLKYFRDEYEAHVNEKRCPAGVCQSLLKYFITADCRGCT
                     KCARICPAGAIEGKVKEIHIINQDKCIKCGACMDACTFHAIIKK"
     gene            982..2793
                     /locus_tag="PLD67_06495"
     CDS             982..2793
                     /locus_tag="PLD67_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOT22204.1"
                     /translation="MDKVNLTINGIEVSVPKDYTVLLAAREAGIDIPTLCYLKDINEI
                     AACRLCIAEVDIKGVPMRNLPATCVLQVQEGMNVRTNTKRVRNAVKMNLELILANHNR
                     ECLTCLRNGTCELQKLCDEMNISDIEYQGTKREAKIDNLSYSIVRDTSKCILCGRCVS
                     TCKEVQGIGILDFTKRGFVTEVAPAFDYSMKDVPCVYCGQCIQACPVAALRERTYIDD
                     VWDAIDDPEKFVVVQTAPGVRASLGEEFGLPVGTDVTGRMVAALKRLGFDKVFDTNFS
                     ADLTILEEGTELISRVKNGGKLPLITSCSPGWIRYCELNYPEFLDNLSTCKSPQQMFG
                     AIVKSYYAEKAGIDPKKIFSVSVMPCTSKKTEANRPEMEVDGLRDVDVSLTTRELGDM
                     IKQARIDFVNLPDEEPDSILGQYSGAGAIFGATGGVMEAALRTVADILTGKDLDNFEY
                     QGVRGLDGVKEASVVLPIDGKDTEIKVAVAHGTANAAKVLEAVKNGEKEYHFIEIMAC
                     PGGCVHGGGQSHVSAKKRLEVNPKSKRADALYELDKSLPLRKSHKNPEVIKLYEEFLK
                     EPNSHVSHKYLHTHYYKRDSYATQEQEEYDKLMEKLK"
BASE COUNT         1046 a          480 c          714 g          806 t
ORIGIN      
        1 ggctgggagc aggtgctttt gtctgtggag aggaaacagc cttgttacag tcattggaag
       61 gcaaaagagg aatgtccaga aataaaccgc catttcctgc taacaaaggg ttgtggggaa
      121 aacctacagt aataaataat gttgaaacat tagcaaatat ccctcaaatt atactcaggg
      181 gacccgaatg gtttaaaagt ttcggtaccg aaaaatcacc gggtacaaaa gtatttgcct
      241 taggcggaaa aattaataat accggccttg tggaagtacc tatgggtact actttaaggg
      301 aggttattta tgatataggc ggaggatgtc ctaacggcaa agaatttaag gcagttcaga
      361 caggaggtcc atcaggcgga tgtctcacca cgaagcactt agatactcca atagattatg
      421 atagtcttac cgcagccggc tctatgatgg gctccggtgg tatgatagta atggatgaag
      481 ataactgtat ggttgatatt gcaagatttt tccttgattt tacggttgat gaatcctgcg
      541 gcaaatgtac tccttgcagg gaaggtacaa aacgaatgct ggaaatttta aataaaatta
      601 cagaaggaaa aggcacaatg gaggatcttg ataagttaga aaaacttgct attaatatca
      661 aggaaacctc actttgcggt ttaggtcaga ctgcaccaga tcctgtatta tcaacattaa
      721 aatatttcag agatgaatat gaagcccatg ttaatgaaaa aagatgcccg gcaggtgttt
      781 gccagtcact tttgaaatat tttattaccg cggattgtag gggatgtaca aaatgtgcga
      841 gaatatgtcc tgcaggagca attgagggta aggttaaaga aattcatata atcaatcagg
      901 ataagtgtat taagtgcggt gcttgtatgg atgcttgtac gttccatgca attatcaaga
      961 agtagaaagg ctggtgcaaa catggataaa gtaaatttaa ctataaacgg aatagaggtc
     1021 agcgtaccga aagattatac agtgcttttg gcagctaggg aagcaggaat agatatacca
     1081 accctttgct atttaaagga tataaatgaa atagccgcgt gcagactatg tattgcagaa
     1141 gttgatataa aaggtgttcc gatgagaaac ctgcctgcaa cctgtgtgct tcaagtacag
     1201 gaaggtatga atgtaaggac gaatacaaaa agagtaagaa atgccgttaa aatgaattta
     1261 gagctgatac ttgcaaatca caacagagaa tgcctcacct gtctgagaaa tggtacctgt
     1321 gagttgcaga agctttgtga tgagatgaat atcagtgaca tagaatatca aggtacaaag
     1381 agagaagcaa aaattgataa tttgtcatat tccattgtaa gagatacaag caaatgtatt
     1441 ctttgcggaa gatgcgtcag cacatgcaaa gaagtacaag gaataggaat attggatttc
     1501 acgaagagag gatttgttac agaagttgct cccgcatttg attacagcat gaaggatgta
     1561 ccctgcgtat attgcggaca gtgcatccag gcatgtccgg tggcagcttt gagagaaaga
     1621 acatatattg acgatgtatg ggatgccata gatgatcccg aaaaatttgt tgtagtacaa
     1681 actgcaccgg gagttcgtgc atcacttgga gaagaattcg ggctgcctgt tggaacagat
     1741 gtgaccggca gaatggttgc agcattaaaa agactcggat tcgacaaagt atttgataca
     1801 aacttttcag cggacctaac aattttggaa gaaggaacag agcttataag cagagttaaa
     1861 aacggcggta agctgccttt aataacttct tgctccccgg gatggattcg ttactgtgaa
     1921 ttaaattatc cagaattttt ggataattta tcaacatgta aatctccaca gcaaatgttt
     1981 ggtgcaattg taaagtccta ctatgctgaa aaagcaggta tagacccgaa aaagatattc
     2041 tctgtatcag taatgccatg tacgtcaaag aaaacagaag caaacagacc tgaaatggaa
     2101 gtagacggac tgagagatgt ggatgtttca ttaactacaa gagaattagg tgatatgatt
     2161 aaacaagcaa gaattgattt tgtcaacctt cctgatgagg aacctgacag catattagga
     2221 caatattcgg gagcaggtgc aatcttcggt gcaacaggcg gagttatgga agcagcgctc
     2281 cgtacggtag cagacatact gacaggtaag gatttggata attttgagta tcaaggcgta
     2341 aggggtcttg atggggttaa ggaagcatcg gtagtactac ctattgacgg aaaggatacc
     2401 gaaattaaag ttgcagttgc tcacggaaca gccaatgcgg caaaggtttt ggaagctgtg
     2461 aaaaatggcg aaaaggaata tcatttcata gaaattatgg catgccccgg gggatgtgtg
     2521 catggaggag gtcaatctca cgtatcagca aagaaaagac ttgaagttaa tccgaaatct
     2581 aaacgtgcag atgcattata tgagcttgac aagtctctgc cacttaggaa gtcgcacaaa
     2641 aatcctgaag taataaaact gtatgaagaa tttttgaagg aacccaacag tcatgtttcc
     2701 cacaagtatc ttcacactca ttactataaa cgagattcct atgcaactca ggaacaggaa
     2761 gaatatgata agctaatgga aaaattgaaa taaaataacc aaaaaaatat tctctgcaga
     2821 cagcagggaa tatttttttg tattattttc attataatca tattgtgtta taatgaatca
     2881 aaaatattat aacctaatgg ttatatgaat aatggatatt gtatcggtgt gaagcaagcc
     2941 gattttgcgg cttgcactta tgatataatg aatataaatc aattcaggtt atactgagct
     3001 aatttacata tatggcaggt gagaggtaaa tatgaactac agaatt
//
LOCUS       DAOSOW010000231         3038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_32349_length_3038_cov_4.006034, whole genome shotgun sequence.
ACCESSION   DAOSOW010000231 DAOSOW010000000
VERSION     DAOSOW010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32349_length_3038_cov_4.006034"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2679)
                     /locus_tag="PLD67_06500"
     CDS             complement(<1..2679)
                     /locus_tag="PLD67_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOT22205.1"
                     /translation="MGKRILSMVLTFMLVITMLPLTSLPVYADSSSGGETLDNMNALQ
                     ALGIDTDEIPEGFDEESEDNPYGKDKITINPVSELFVSEQSSGVDGYKMSRSLRGHEG
                     ILTGTTNDFYSNTKNSTNNLITLDSNKDISTEYYKSYKAFKAVSGNFDGKGMKGQVVT
                     VAAGANANDGGLYMYFTDPVSGEMSIEPIELMGTGDIIGNYDRYDYEDLTTQFYQLQN
                     YLQISTGDYDGDGIDEVAVYIPEQGNSQVAIYKLQTTDSNPGNLYLNTSKWSIAWTYA
                     FNESPYVSNMVSITSGDFNRDGTDDIALTWGTYYGPNYNTPCKAAVLYGSKLGNMLQK
                     SKTFDLIYANSQIVRAAFTYGDINGDGTDELILGGQLVSDINKKVFSNRVIMMYNYNG
                     DEDRFVLTYSENFEIIDEDQQGEDNKYFSSPACVANITTVKMNGVGKAAHIYLDSVIF
                     EYGNDGLKIVLELDEADEMKDTRPFSTYNDYAEYGVLAADFDGDGMETLQVMQYFFPQ
                     TLTYKDFWWWFFGTFDPVNIPGNLFMVSIYASGEDLIYNRKDCGEYLTTYYTNVNTDD
                     DTTFIDYTGNHYIAYSDPKVLAVLASPPYFEDIAIQEDSGNYLEGSTEYSSTGGYSTG
                     QTTSHTLSVGTYISFEQEFSVFGIKVASFETEFAYSHGFTWETEKTSTLEQSVTYGTA
                     TGEDAVAFYSIPVEVYVYKSYYPEINPDGTATMKEQTMTVNIPHTAAVVTMPLKTYES
                     IAADYEELPRISGRVLNHTLGKPSTYPFSVSEFAAQLGSGIYTGANSAIVFGGDWAAV
                     NYGGLGYVTQEIAMSNESSSSFSNSESIDFKIGGGAGGLVVGITAGYEYGYGTTTVTT
                     NGNSFSGTVNNLPTGAEDYYYAWKLFAYYYSDGMNAF"
BASE COUNT          843 a          645 c          565 g          985 t
ORIGIN      
        1 aaatgcattc atgccgtcag agtagtaata tgcgaaaagc ttccaggcat aatagtaatc
       61 ttcagcccct gttggcaaat tattcactgt tcccgagaaa ctgtttccat tggtcgtcac
      121 tgttgttgta ccgtaaccgt attcatagcc ggcagtgatt cctactacta aaccccctgc
      181 tccgcctcct attttaaaat ctatgctttc agaattggaa aagctgctgg aggattcgtt
      241 gctcattgca atttcttggg tgacatatcc caagcctcca tagttaactg ctgcccaatc
      301 tcctccaaat actattgcgc tattagcacc ggtataaatg cctgacccaa gttgggcggc
      361 aaattcagag acagaaaatg gatatgtgga aggcttgccc agggtatggt ttaaaactct
      421 ccctgaaatt cgaggcagtt cttcataatc ggctgctatg gattcatagg ttttcaaagg
      481 cattgtaact actgcagccg tatgaggtat atttacggtc attgtctgct ctttcatggt
      541 cgcagtaccg tccgggttaa tttcgggata atatgatttg taaacataaa cttccacagg
      601 tatcgaataa aaggctactg catcttctcc cgtggctgtc ccgtatgtaa cggactgttc
      661 cagggtgctg gttttttcag tttcccaggt gaatccgtgg ctgtatgcaa actcggtttc
      721 aaatgaagcg acttttattc cgaaaacgga aaattcttgt tcaaaactta tataagtgcc
      781 cacagatagg gtatgagagg tggtttggcc tgtagagtag ccccctgtcg aagagtattc
      841 ggtggagcct tccaaataat tgccgctgtc ttcctgaatc gcaatatctt caaaatacgg
      901 aggcgatgcc agcacagcca gcactttagg gtcggaataa gcaatatagt ggtttcctgt
      961 ataatctata aatgtagtat catcatcggt atttacattt gtataatatg tggttaaata
     1021 ttcgccgcaa tcttttctat tgtagataag atcctctccg cttgcatata tggagaccat
     1081 aaacaaattg ccgggtatgt ttaccggatc aaaggtacca aaaaaccacc accaaaaatc
     1141 tttgtaagtt aatgtctggg ggaagaaata ctgcataacc tgtagtgttt ccatgccgtc
     1201 tccatcaaaa tcagccgcaa gaactccata ttcggcataa tcattgtatg tggaaaaggg
     1261 acgagtatct ttcatctcat ctgcttcatc caattcaagg acaattttta ggccgtcgtt
     1321 tccatactca aagattacgc tgtccagata tatatgggcg gccttcccaa ccccgttcat
     1381 cttaaccgtt gtaatattgg ctacacaagc aggagaactg aaatatttat tatcttcccc
     1441 ttgttggtct tcgtcaataa tttcgaagtt ctcggaatat gtaaggacaa atctgtcctc
     1501 atctccgtta taattgtaca tcataataac ccggttgctg aatacttttt tgttaatatc
     1561 ggaaaccagc tgtccgccta atatcagttc gtccgttccg tcaccgttta tatcaccgta
     1621 ggtaaatgca gcacgaacta tttgggaatt agcatatatc aaatcaaagg ttttgctctt
     1681 ttgaagcatg tttccaagct tgcttccgta aagaacagct gccttgcatg gagtgttgta
     1741 attaggtcca tagtaggtgc cccaggtcaa agctatgtca tccgtgccat ccctgttgaa
     1801 atctcccgat gtaattgaaa ccatattgga aacataaggg gattcattga atgcataggt
     1861 ccaggctatg ctccacttgc ttgtattaag ataaaggttg cccggatttg aatccgttgt
     1921 ttgaagttta tatatggcta cctgagaatt accctgctcc ggtatgtata cggcaacttc
     1981 gtcaataccg tctccgtcat aatcaccagt tgatatctgc aggtagtttt gaagctgata
     2041 aaattgtgtt gtcaaatctt catagtcata tctatcatag ttgcctataa tatcacccgt
     2101 gcccatgagt tctataggtt caatgctcat ttcacctgat acggggtcgg taaaatacat
     2161 atatagcccg ccgtcattag catttgctcc ggcagctacc gttacaacct gccccttcat
     2221 tcccttaccg tcaaaattgc cggaaacagc cttgaaagcc ttataggatt tgtaatattc
     2281 tgtcgaaatg tctttgttgc tatccaaggt aataagattg ttggttgaat tctttgtgtt
     2341 agaataaaaa tcattggttg tgccggtcaa tataccttcg tgtcccctta agcttctgct
     2401 cattttgtac ccatcaactc cagatgattg ctctgaaaca aataattccg atacaggatt
     2461 tattgtaatc ttgtctttgc catatgggtt gtcttcactt tcttcatcaa aaccttcagg
     2521 aatttcatca gtatctattc ccaatgcctg caaagcattc atgttgtcca atgtttctcc
     2581 acccgaactg ctgtctgcat aaaccgggag gcttgtgagg ggtaacattg taataacaag
     2641 cataaatgtt aaaaccatgc ttagaattcg ttttcccatt acttatagcc cctttatatt
     2701 ttattttttt aaaaaataac aggataatta attatatatt catttattta aaaaaacaaa
     2761 acattttttg aaattattca catttttgtc tttttattct aaaataataa ttcgttctag
     2821 tttcacaatt ttcttcataa ttattgttga taaataccat aatactgcct aaaaatcttg
     2881 ggtaatgtat tgtcacactt gaatctccgc tgagagttgt agaatgtata cccaaaaaca
     2941 caaacagcgc ctgatatcat aataattgat aatcaggcgc tgcttattat aggatgtttt
     3001 gtccttattg ttttataaag actatgtttt catccccc
//
LOCUS       DAOSOW010000232         3025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_32518_length_3025_cov_4.150168, whole genome shotgun sequence.
ACCESSION   DAOSOW010000232 DAOSOW010000000
VERSION     DAOSOW010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32518_length_3025_cov_4.150168"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="PLD67_06505"
     CDS             <1..917
                     /locus_tag="PLD67_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOT22206.1"
                     /translation="GFTGLFSLGQAGFMAIGAYTVAIFTVPLAARAKIFYITPQNPVL
                     AQIELPFVIALILGGLLAALVALLIGLPVLRLKSDYLAIATLGFSEIIRIVFTNIKSI
                     TNGALGIKNIPTMPTMWVFFGIAIISIGFMWLLINSSYGRAFKAIREDEVAAEAMGIN
                     LFKHKVMSFSIGAFFAGIGGGLLATLFATVDPNQFYFPVTYNFLLIIVLGGMGSISGT
                     IIASFVVTSGLEILRFFDEPLSLFGLNIPLFRPGLRMVIFSILLMVLVLFFRNGLLGQ
                     KELSWKLIFDLLSGKKPRKKTGEGGAAQ"
     gene            914..1684
                     /locus_tag="PLD67_06510"
     CDS             914..1684
                     /locus_tag="PLD67_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010074112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT22207.1"
                     /translation="MSVLKINNVTMQFGGVTAVNNLNIEVQEGRIEALIGPNGAGKTT
                     AFNVITGVYSPTKGNVFYKNENITGLTPDKITKKGIARTFQNIRLFKNLTVLENILIA
                     NHLRINSNFLSSTLRMPWSNKEERQMREKSEMLLDKLDLLRLKNEIAHSLPYGEQRKL
                     EIARALATDADLLLLDEPAAGMNPSETMELSSFIHNIRDEFHLTIFMIEHHMDLVMEI
                     SEKIYVLDFGQLIAEGTANEVKNDQRVIEAYLGVEEDA"
     gene            1677..2384
                     /locus_tag="PLD67_06515"
     CDS             1677..2384
                     /locus_tag="PLD67_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002586268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT22208.1"
                     /translation="MLKINGLHVNYGGISAVKGISIHVPEKSIVTLIGANGAGKSTTL
                     KAIAGLVKARNGEIIFQNENILGKDTIDIVARGITMVPEGRKVFVNLSVLENLRVGAY
                     LRRDGIEDDIKRVYDLFPILKARAWQLAGTLSGGEQQMLAVGRALMARPKLIMMDEPS
                     LGLAPLIVNSIFEIIKEINKQGTTILLIEQNANKALKVADYGYVLETGNITMEGKGIE
                     LLKNEKIIEAYLGTSKK"
     gene            complement(2469..>3025)
                     /locus_tag="PLD67_06520"
     CDS             complement(2469..>3025)
                     /locus_tag="PLD67_06520"
                     /inference="COORDINATES: protein motif:HMM:NF037962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HOT22209.1"
                     /translation="VKAIFAVIAGAMADFIFKGFFNKSNNEGIASSIHHHVCTDCGCH
                     DDPGILKPALKHTINIFIFLFITNFLINFFIEYVGEDNLSEILLHDSIFQPALAGIIG
                     FIPNCAASIILTQLFIAGSISFGSVIAGLSTGAGVGLLILFKVNRNINENLKIMLYIY
                     VFSVLAGSLIQTMGTYLTVPHGFF"
BASE COUNT         1012 a          528 c          620 g          865 t
ORIGIN      
        1 acggttttac gggattattc tcattgggac aggcaggttt tatggcaatc ggcgcttaca
       61 cggttgcaat atttactgtt cccttagccg ccagagctaa aatattttat attactcctc
      121 aaaatcctgt gttggcacaa attgagctgc catttgttat tgcacttata ctgggaggtc
      181 ttttagcagc cttggtagca cttttaatag gtttacccgt tctcaggtta aagagcgact
      241 atttagcaat agcaacatta ggattttcgg aaataatccg tattgttttt acaaacatta
      301 aatcaattac caacggtgca ttgggaatta aaaatatacc tacaatgcct accatgtggg
      361 ttttctttgg aatagccata atttcaatcg gttttatgtg gctgttaata aactccagct
      421 acggcagggc ttttaaagcg ataagagagg atgaagtggc agcagaagcc atgggtatca
      481 atttgtttaa acataaagta atgtcattca gcataggagc attttttgca ggaatcggag
      541 gcggactatt ggctacactg tttgctacgg tagaccctaa tcaattttat ttccctgtta
      601 cctacaactt ccttttgatt atcgtattgg gaggtatggg aagtatatca ggtacgatca
      661 tagcatcctt tgtagttacg tcaggacttg aaatactaag atttttcgat gaacccctat
      721 cattgtttgg attaaatatt ccattattcc gtcccggttt aagaatggta atattctcaa
      781 tattattgat ggtgttggtg ctcttcttca gaaacggcct attaggacag aaggaattgt
      841 cctggaagtt gatttttgac ctgctttccg ggaaaaaacc acgaaaaaag accggtgaag
      901 gaggtgctgc acaatgagtg ttttaaagat aaacaatgta actatgcaat ttggtggagt
      961 tactgctgtc aacaacctta atattgaggt tcaagaggga agaattgaag ccttgatagg
     1021 tcccaacggt gcaggaaaaa caaccgcctt taatgttatt accggtgtat attctccaac
     1081 taaaggaaat gtattttata aaaatgaaaa catcacaggc cttacacccg ataaaataac
     1141 caaaaaaggt atagctcgca catttcagaa tatcagatta ttcaaaaatt taaccgtgct
     1201 tgaaaatata ctgattgcca atcatttaag aataaacagc aatttcctgt ccagcacact
     1261 tagaatgcct tggtctaata aagaagaaag acaaatgcgt gaaaaatcgg agatgcttct
     1321 agacaaattg gatttgttaa gattgaaaaa tgaaattgct cattctcttc catatggtga
     1381 gcagcgaaaa ttggaaatag ccagagctct tgcaactgat gccgaccttc tgctcttgga
     1441 tgagccggct gcaggtatga atccctcgga aacaatggag ttgtcatcat tcattcataa
     1501 tataagagat gaatttcatc ttacgatttt tatgattgag catcatatgg acctggttat
     1561 ggaaatatct gaaaaaatct acgttttaga cttcgggcag cttatagcag aaggaacggc
     1621 aaatgaagtt aaaaatgacc aaagagttat agaggcttac ttgggggtgg aagaagatgc
     1681 ttaaaattaa tggactacat gtaaattacg ggggaatttc cgcagttaag ggtataagca
     1741 tacatgttcc ggaaaaatca atcgtaacat taataggagc caacggtgcc ggtaaaagca
     1801 ccacccttaa agccatagca ggacttgtta aagcaaggaa cggcgaaatt atttttcaaa
     1861 atgaaaacat actcggaaaa gatacaatag atattgtagc aagaggaatt accatggttc
     1921 cggaaggaag aaaagtcttt gtgaatcttt cggtgctgga aaacctaaga gtcggtgcct
     1981 acttgagaag agacggaatt gaagatgata taaaaagagt ttatgatttg tttccaatat
     2041 taaaagctag agcttggcaa ttggcaggta ccttatctgg aggcgagcag cagatgctgg
     2101 cagtaggaag agccttaatg gcaaggccta agctgataat gatggatgag ccttccttgg
     2161 gattagctcc cttaatagtt aattcaatat ttgaaattat taaagaaata aacaaacaag
     2221 gaactacaat tttgttaatc gagcaaaatg ctaacaaggc actgaaggtt gcagattacg
     2281 gttatgtctt ggaaacgggc aatataacca tggaaggaaa aggaattgag cttctgaaaa
     2341 acgagaaaat tatagaagca tatttaggaa catcaaaaaa ataaagaaac gggacagtgg
     2401 ggaactacct tactgtcccg attttgtaat atccaagata cgtccccatt tccacgtcgt
     2461 ttcctgaatc aaaaaaaccc atgtgggaca gtaaggtacg tccccattgt ttgaatcaaa
     2521 gaccctgcca atacagaaaa aacatatata tacaacataa tcttgaggtt ttcattaata
     2581 tttcgattta ccttgaataa aatcaacaac ccaactcctg ctcccgtgga gaggccggca
     2641 attaccgagc caaagcttat gcttcctgcg ataaacagct gagtgagtat aattgatgcg
     2701 gcgcaatttg gtataaatcc tattatacct gccaaggcag gctgaaaaat actgtcatgt
     2761 agtaaaattt cagataaatt atcctcgcct acatactcta tgaaaaaatt tataagaaaa
     2821 ttagtaataa acaaaaatat aaatatattg attgtatgct tcaatgctgg ctttaatata
     2881 cccgggtcgt catggcatcc gcagtcggtg caaacatggt gatgaataga acttgcaatc
     2941 ccttcattat tgcttttatt aaaaaaacct ttaaaaatga aatccgccat agctccggct
     3001 ataacagcaa atattgcttt tacgg
//
LOCUS       DAOSOW010000233         3025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_32521_length_3025_cov_3.711448, whole genome shotgun sequence.
ACCESSION   DAOSOW010000233 DAOSOW010000000
VERSION     DAOSOW010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32521_length_3025_cov_3.711448"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLD67_06525"
     CDS             <1..460
                     /locus_tag="PLD67_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011723036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOT22210.1"
                     /translation="CDGRAKSRNYYTEFAKALPKDTVILTAGCAKYKYNKLNLGDIGG
                     IPRVLDAGQCNDSYSLALIALKLKEVFELQDINELPLAFNIAWYEQKAVIVLLALLYL
                     GVKNIHLGPTLPAFLSPNVANVLVEKFGIAGIGTVEDDLKIFLGDSTVTA"
     assembly_gap    476..485
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            506..715
                     /locus_tag="PLD67_06530"
     CDS             506..715
                     /locus_tag="PLD67_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HOT22211.1"
                     /translation="MAKEITKDMYIGEILQIDPSLAEILMEAGMHCLGCPSSQRESIE
                     EAAMVHGFDADELVKKLNSHINSAK"
     gene            976..1248
                     /locus_tag="PLD67_06535"
     CDS             976..1248
                     /locus_tag="PLD67_06535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009288905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT22212.1"
                     /translation="MEFKYEKDRIYLVNQVGESIAEVTFPQISENEVNINHTYVNPAL
                     RGQGIADKLVYELASYLRKNNIKAISTCSYAIDWFEKHPEFKDVCK"
     misc_binding    1308..1543
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1785..2567
                     /locus_tag="PLD67_06540"
     CDS             1785..2567
                     /locus_tag="PLD67_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005216079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT22213.1"
                     /translation="MKKNVSKKVKLAVLLILTAILAVGCAAQSSQPETEELIMGLDDT
                     FAPMGFRDEKGELVGFDVDLANEVAERIGVTIKFQPIDWSMKETELNAGNIDLIWNGY
                     TITAERQEKVAFTKPYLENSQIIVTLADSDINTKVDLAGKNVAVQAESSALDAINSEP
                     EVAESFGELVEFSTNNEAFSDLESGRTDALVVDEVNARYFMKQVGEEKYKVLDEDFGD
                     EEYGIGLRKEDTELLKKINDAMDEMKKDGTYDAIYAKWFSEN"
     gene            2579..>3025
                     /locus_tag="PLD67_06545"
     CDS             2579..>3025
                     /locus_tag="PLD67_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HOT22214.1"
                     /translation="MEAKVLRMLVPMALEGLKVTMGVFAVTLIMSLPLAIVVAKLRMS
                     KNKIVSKITGTYIYIMRGTPLLLQLMFIFFGFYRVPYIGYPFSREQAIVIAFVLNYTA
                     YFAEIFRGGINSIDVGQQEASLVLGLSKTHTFMKIILPQVIKNVMPS"
BASE COUNT         1086 a          444 c          628 g          857 t
ORIGIN      
        1 ctgcgatggc agagctaaat caagaaacta ttacacggaa tttgcaaaag ctttacccaa
       61 agatacggtt attttaacag ccggctgcgc aaaatataaa tacaataaat taaatttagg
      121 tgatataggc ggaataccaa gggttcttga tgcagggcag tgtaacgatt catattcatt
      181 ggctcttata gcgcttaaat taaaggaagt atttgaatta caggatataa atgaattgcc
      241 cttagcattt aacattgcat ggtatgagca aaaagcggtt atagtactct tggcgcttct
      301 atatttagga gttaagaata ttcacttagg accgacttta cctgctttcc tttcaccaaa
      361 tgtcgcaaat gtattagttg aaaaatttgg tatagcagga atcggtacag tagaagacga
      421 tttaaaaata ttcttgggag actcaactgt tactgcttaa tgaattttaa ttaatnnnnn
      481 nnnnngagaa caaaggaggt tctttatggc aaaagaaata actaaggata tgtatatagg
      541 agaaatactg caaatagacc caagtttagc cgaaatatta atggaagcag gaatgcactg
      601 cctcggatgt ccttcttcac aaagagaatc aattgaagaa gcagccatgg tgcatggttt
      661 tgatgcggat gaactggtta aaaagttaaa ttctcatata aactctgcca aataaattat
      721 aagagagcat ggacaacatg ctctcttgtt tcatgctatg aatggtttat aaaaaatgta
      781 tccctattaa tatccaacag gaaaattaaa cattaaaatt tcaattgtaa ataatattaa
      841 aagaaaggca ggtgacaaac ctgattcgaa accgcccaac aataaataaa tatgttgtcg
      901 ggtactcgga aatcttgttg taaatgcaat aagataaaaa gcataaggat aatattatgt
      961 ttagaaccag gaactatgga atttaaatat gaaaaagaca ggatatacct agtaaaccaa
     1021 gtaggagaaa gtattgctga agtaactttt ccccaaatat ccgaaaatga agttaacata
     1081 aatcatactt atgtaaaccc cgctctacga ggtcaaggca tcgcagataa attagtttat
     1141 gaattggctt catatctgag aaaaaataat ataaaagcaa tatcaacatg ctcctatgcg
     1201 atagactggt ttgaaaaaca tccggaattc aaagatgtgt gcaaataaaa atatttttta
     1261 aaaaacactt tacatttata aatatttgtg ataatgtatg taaaatgata aatgccgtga
     1321 caaggaatag tagatataaa agttttttat agagagtgca tggtaggtgg aaatgcataa
     1381 aaacctatat tgaacacacc tttgagcaaa tggatgaaat tcagtagtct tattcggaaa
     1441 tgccaccgtt acagggcaag agtatgttag tacttagtga ggctgatgct gtgaagtatc
     1501 agcgaagtga ggtggaacca cgtgaatttc tctcgtcctc taggaaatcc taggacgaga
     1561 gtttttttta tagacttgac ccaatttatt tgaccgaagg gaaaagaaat tgttggtaag
     1621 tcttacacag cgcaattcca atttgcgcga caatcatagt atggggacac acctcttcta
     1681 tggggtaagt ctttgcagat agaggaatgt ccccaaataa gcaaattggt gatggagcct
     1741 gtgatattat tattatgatt attaaataag aggagaagag aaaaatgaaa aaaaatgtat
     1801 caaaaaaagt taaattggcg gtattgctta ttttaaccgc aatcttggct gtaggttgtg
     1861 cagcacaatc atcacaacct gaaactgaag agctaataat gggacttgat gatacctttg
     1921 cgcccatggg tttcagagat gaaaaaggag aattggtagg atttgatgtg gacttagcaa
     1981 atgaggttgc agaaagaatc ggtgtaacaa ttaaattcca gcctatcgac tggtcaatga
     2041 aggaaaccga attaaatgca ggaaatattg atttaatttg gaacggctac accataactg
     2101 ccgagagaca agaaaaagtg gcatttacta aaccttactt ggaaaacagt caaataatcg
     2161 tgaccttagc cgactcagat attaatacaa aagttgactt ggcaggtaaa aatgtcgccg
     2221 tgcaagctga atccagtgca ttggatgcaa taaattccga gccagaggta gctgaatcct
     2281 ttggggaatt agtagagttt tccacaaaca acgaagcatt cagcgatttg gaatcagggc
     2341 gtacagatgc tttggtagta gatgaagtaa atgcgagata cttcatgaag caagtcggtg
     2401 aggaaaaata caaagtatta gatgaagact ttggtgatga agaatatggt atcggcttga
     2461 gaaaagaaga tacagagtta ttgaaaaaaa taaacgacgc aatggacgaa atgaaaaaag
     2521 acggaacata tgatgctatt tatgcaaaat ggttttctga aaattaaagg aatgtaatat
     2581 ggaagcaaaa gttttgagaa tgcttgtgcc catggcattg gaagggctaa aggtaactat
     2641 gggggttttt gcggttacat tgattatgtc ccttcctttg gccatagtgg ttgcaaagct
     2701 tagaatgtca aaaaacaaaa tagtttcaaa aattacggga acttatattt atataatgag
     2761 gggtacgccg ttactattgc agttaatgtt tatattcttc ggattttatc gtgtaccgta
     2821 cattggatat cccttcagca gagaacaggc tattgtcatt gcttttgttt taaattatac
     2881 tgcttatttt gcagaaatat tcagaggagg aataaattcc atagatgtcg gtcaacagga
     2941 agcatctctt gtattgggct taagcaagac acatacattt atgaaaatta ttctccctca
     3001 ggttataaaa aatgttatgc cctca
//
LOCUS       DAOSOW010000234         3016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_32642_length_3016_cov_4.116852, whole genome shotgun sequence.
ACCESSION   DAOSOW010000234 DAOSOW010000000
VERSION     DAOSOW010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32642_length_3016_cov_4.116852"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1938)
                     /locus_tag="PLD67_06550"
     CDS             complement(<1..1938)
                     /locus_tag="PLD67_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22215.1"
                     /translation="MSLKIIAGRANTGKSSFIYDEIKTQSKNSKAKLILIVPELMTYQ
                     AESNIIERFDLPGIMNIEILSFKRLERKILEEVGGLKMQDLSVFGKIMLLKKIFEENK
                     DKLKVYGNSYKQSGFLKEFEELITEFKQNLVEGQSLVQAAQDMDDEILIRKLSDIQLI
                     YSEFIERTKNKYFDEEDKTNLFISLIKESAYIRNSNIWIDGFESFNRQRLHLIKNLCD
                     YSKSVTLSLNIDSSYLDNLEEKDDFEAFKIIYDTYHTLKNFDTDIEIISLSENKSPST
                     EIKAIEKNIFSLNIEEYENNTDKINIFSSLNPYSETQKTAANIISLVRDENLRWRDIK
                     VAVGNMEVYETNIKRIFSQFEIPFFLDVKRDILNNPLSKYILSILDMFIWNFKHDNVF
                     EYLKTGFSPLNINQVQYLENYALQFGIEGDKWFREFKSFDKDGNQITGEKNNIERYRK
                     LFAEDFEVERKKIHNLKTANEITKLIFKYLDKHKIKEKMEKAVKEFNNAQKYEEASEY
                     SQCWNYVMEIFEQLLLIGQDTEISLNDYRKMLEAGLGEVEVSIIPPTIDKVEVGTIDR
                     IAVTKPKALFIIGANDSNLDTKNTDKGLLLNEERDILLENDVRLTKGADYETSKEKHM
                     LYKLFSSPSHSLSVSYPLGLEN"
     gene            2147..2821
                     /locus_tag="PLD67_06555"
     CDS             2147..2821
                     /locus_tag="PLD67_06555"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HOT22216.1"
                     /translation="MKNYCYNAEINMYEYRKTFSYNNTEMLKLTIIYPQISLYNLQAE
                     YKINTRINMDVSNFKRYAKYLFKQAIKSYTESQNKGYPFREYEAYMDYSITYNENCFL
                     SLYYDKYEFTGGAHGSTVRASDTWELCLGTYLPLNSFFRPGTDYRHLLINEMIKQAEV
                     IQNQQNIYFDDYKSLIIKNFDEDSFYLTPSGITIYCQQYDIAPYSTGIVEFTIPYSVI
                     GWRPNC"
BASE COUNT          972 a          521 c          420 g         1103 t
ORIGIN      
        1 gttttctaag ccgaggggat aactgacgga caaactatgg gaaggacttg aaaacaactt
       61 gtaaagcata tgtttttctt tagaggtttc ataatctgct ccttttgtaa gtcttacatc
      121 attttcaaga aggatatctc tttcttcgtt taataataac cccttatctg tattcttagt
      181 atcaagattg ctgtcgtttg ccccaataat aaacaatgcc tttggctttg taacggcaat
      241 tctgtcaatt gtccctactt caaccttatc gatagtaggc ggaattatac ttacctcaac
      301 ttctccaagt cctgcttcca acatttttct atagtcattt aaagaaattt cggtatcctg
      361 cccaatcaat aaaagctgct caaaaatttc cattacatag ttccagcact gagaatattc
      421 gcttgcttcc tcatattttt gagcattatt aaattctttg acagccttct ccattttttc
      481 ttttatcttg tgtttgtcta aatatttgaa tattagtttt gtaatttcat tggcagtttt
      541 aagattatga attttttttc tttcaacttc aaaatcttcg gcaaataatt ttctgtaccg
      601 ctcaatgtta ttcttttcac cagtaatttg attgccatcc ttatcaaaag acttaaattc
      661 tctaaaccat ttatctccct caataccaaa ctgcagcgca taattttcca aatattgaac
      721 ttggttaatg tttaaaggtg aaaatcctgt ttttaaatat tcaaaaacat tgtcatgttt
      781 aaagttccaa ataaacatat caagaattga aagtatatac ttgcttaaag ggttgtttaa
      841 aatatctctt tttacatcca agaaaaatgg tatttcaaat tgagaaaaaa ttctttttat
      901 atttgtttcg taaacttcca tattcccaac tgcaaccttt atatctctcc atcttaaatt
      961 ttcgtcccga accaaggata taatattagc tgctgttttt tgtgtttccg aatatggatt
     1021 taaagaagag aaaatattta ttttatctgt attattttca tattcttcga tatttaaaga
     1081 aaatatgttt ttttcaattg cttttatttc agttgaaggt gatttgtttt cgcttaggga
     1141 tataatttct atatcagtat caaaattttt taaagtatgg tatgtatcat aaataatttt
     1201 aaaggcttca aaatcgtcct tttcctctaa attatctaaa taagaagaat cgatatttaa
     1261 tgataatgtt acggattttg aataatcaca aaggttctta attaaatgaa gtctttggcg
     1321 gttaaaactt tcgaaaccgt caatccatat attagagttt cttatatatg ctgattcttt
     1381 aatcagagaa ataaaaagat ttgttttatc ttcttcatca aaatacttat ttttggttct
     1441 ttctataaat tcagaataaa tcaattgaat atccgataat tttcgtatta gaatttcatc
     1501 gtccatatct tgagctgctt gaactaatga ctggccttca actaagtttt gtttgaattc
     1561 agttatcaat tcttcaaatt cttttaaaaa tccgctttgc ttgtatgaat tgccataaac
     1621 tttaagtttg tccttatttt cttcaaatat tttttttaga agcatgattt tgccgaaaac
     1681 acttaaatcc tgcattttca ggcctccaac ttcttcaagt atcttcctct caagtctttt
     1741 aaagcttaaa atctcaatat tcataatgcc gggcaaatca aatctctcaa taatgttgct
     1801 ttcagcttga taggtcataa gttcaggcac aataagtatt aacttggctt tagaattttt
     1861 tgattgtgtt ttaatttcat cataaatgaa tgatgatttt cccgtgtttg ctcttcctgc
     1921 tattattttt aaactcatat cctatgcctc tatatacgga aatgtatttt cttaattata
     1981 caaggatttt tcgtttatgg caattaaatt ccgattgggg acattcctcc tgccagacgg
     2041 gcattcccga atatggagcg tatccccatc actataaaaa tagggttagt caatctttga
     2101 accataaaat aattctcaca tattataatt caaggagggt tatgaaatga aaaattattg
     2161 ctataatgca gaaataaaca tgtatgaata cagaaaaaca ttcagctata acaataccga
     2221 aatgcttaaa ttgacaatta tatatccgca aatcagttta tataacttac aggcggaata
     2281 caaaataaat actcgcatca atatggacgt aagtaacttt aaaagatatg ccaaatattt
     2341 atttaaacaa gcaataaaat catacacaga gtcccaaaac aagggctatc cgtttaggga
     2401 gtatgaagct tatatggatt attcaataac atataacgaa aattgttttt taagcctgta
     2461 ctatgacaaa tatgaattta cgggaggagc acacggtagc accgtaaggg cttcagatac
     2521 ttgggagtta tgtttaggaa cctatctgcc tctgaacagt ttcttcagac cgggaacaga
     2581 ctacagacat ctattgatta atgaaatgat aaagcaagca gaagttatac aaaatcagca
     2641 aaatatatat tttgatgatt acaaatcttt gattataaaa aattttgatg aagacagttt
     2701 ttatcttact ccttcaggca ttacaattta ttgtcagcaa tacgatattg caccttactc
     2761 aaccggcatt gttgaattta caattcctta ttcagtgata ggctggagac caaactgctg
     2821 ataaaaaaga gtccgccttg tgcgggctca atttatacga tcctttttaa agcataaggt
     2881 aaacatttga caatatcccc tgcagtcagt gaatactccc ctatatattc tgctcctata
     2941 tcgccggcta agccatgtaa atacactgca gccttcaatg cattcttaat attaatccct
     3001 tgtcctataa atgaag
//
LOCUS       DAOSOW010000235         3003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_32827_length_3003_cov_3.158073, whole genome shotgun sequence.
ACCESSION   DAOSOW010000235 DAOSOW010000000
VERSION     DAOSOW010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32827_length_3003_cov_3.158073"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PLD67_06560"
     CDS             complement(<1..213)
                     /locus_tag="PLD67_06560"
                     /inference="COORDINATES: protein motif:HMM:NF016365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 family protein"
                     /protein_id="HOT22217.1"
                     /translation="MIGTIANALAIIVGGIIGLLFKNIIPEKISEALLKVIGLAVIAI
                     GINLMLSGENFTLLIISLVIGTIKGEI"
     gene            complement(219..1085)
                     /gene="rsmI"
                     /locus_tag="PLD67_06565"
     CDS             complement(219..1085)
                     /gene="rsmI"
                     /locus_tag="PLD67_06565"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOT22218.1"
                     /translation="MSKLYVVPTPIGNLGDMTNRAVETLNSCDLILAEDTRHTQKLLN
                     YFNIKTKMISYHKFNEKQRSEEIIHKLLNEDINAALVTDAGTPCISDPGYELVREVRE
                     KGIEVIGLTGASALTTALSISGIETYEFAFYGFLPRKKSELDTALKRVLNNPVKTFVF
                     YESPKRIISLIENIKIHMPNSTLCVCKELTKIHEKTFYGSVDDVLEQLNNHGDVEKGE
                     YVIVGYNNDYKEKGDTDIQVSLHAILFDKLFKNKYSLKEAVNEAALEGIASKNEAYKA
                     SLEIKKIIEKLR"
     gene            complement(1259..1341)
                     /locus_tag="PLD67_06570"
     tRNA            complement(1259..1341)
                     /locus_tag="PLD67_06570"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1306..1308),aa:Leu,seq:caa)
     gene            complement(1401..2624)
                     /locus_tag="PLD67_06575"
     CDS             complement(1401..2624)
                     /locus_tag="PLD67_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012860201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOT22219.1"
                     /translation="MYDSVIISLLSLSLIINIFLLIKQIKNNTNERIISELKIYLKDN
                     NLDLIDSVNRKIAESEQKQLRELLTNKLETVERIDRNSNKLTESFAKFSADTSKTLNE
                     NFISLNERVSQNLDRINLRVEERLNEGFEKTNKTFVNILERLSRIDEAQKKIDSLSTN
                     IVSLQDVLTDKKSRGTFGEVQLNHILSVVFGEKNDKIYEIQKKLENSMIADAVLYIPE
                     PVGMLCIDSKFPLENYQRMIDVNISEAERKQYSRDFKLNVKMHINDISSKYIIPGQTS
                     DQAIMFIPAEAIFAEINAYHQDLLDYAGEKKVWITSPTTLMSVLSTVQVVLRNIEREK
                     YAGIIHEELNKLGKEFKLYKDRWESLSRNIKRVSDDVDKINITSNKIERKFDRISRVE
                     MGCEPEIAEIEYSEI"
     gene            complement(2751..>3003)
                     /locus_tag="PLD67_06580"
     CDS             complement(2751..>3003)
                     /locus_tag="PLD67_06580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT22220.1"
                     /translation="SLYDDKTNVLIYFSDDGIGIPSHLAKNIFKPFVRIDDSRNSQTG
                     GTGLGLSIAYKIIKSHGGDMELKTDLNKGCTYIITLPKI"
BASE COUNT          880 a          571 c          415 g         1137 t
ORIGIN      
        1 aatttcgcct tttattgtac ctattaccaa tgatatgatt aaaagcgtaa aattttcacc
       61 ggatagcatc aaatttatgc ctatggcaat tacagccaag ccgataacct ttagaagcgc
      121 ttcggatatt ttttcaggta ttatgttttt aaataaaagt cctattattc cgcctactat
      181 tattgcaagt gcatttgcga ttgtgcctat catatgccct accttaactt ctcaataatt
      241 ttttttattt ccagcgaagc cttatacgct tcatttttag aagcaattcc ttctaatgct
      301 gcctcattta cggcttcttt taaagaatat ttatttttga ataatttgtc aaataaaatt
      361 gcatggaggc ttacctgtat gtctgtatca cccttttctt tataatcatt gttatatcct
      421 acaataacgt attcgccttt ttccacatca ccatgattgt ttaactgctc aagtacatca
      481 tccacacttc cataaaaagt tttttcatga atctttgtca attccttgca aacacaaaga
      541 gttgaattcg gcatatgaat ttttatattt tcaatcaaac ttattatcct ctttggagat
      601 tcataaaaaa caaatgtttt aacgggattg ttaagcactc tttttaatgc agtatccagt
      661 tcgctttttt tacggggcaa aaatccgtaa aatgcaaatt cgtatgtttc aattccagat
      721 atggaaaggg ctgttgtaag agcagatgca ccggttaaac ctattacttc aatacccttt
      781 tccctgactt ccctcactaa ttcatatccg gggtctgaaa tacacggtgt tcctgcatct
      841 gttaccaagg ctgcattgat atcctcattc aaaagcttat gtattatttc ttcgcttctt
      901 tgcttttcat taaatttatg atatgaaatc atttttgttt taatattgaa ataatttaac
      961 agcttttgag tatgcctggt atcttcagct aatattaaat cacaagaatt cagtgtttca
     1021 actgccctgt ttgtcatatc gcctaaattc ccgataggcg taggtaccac atataattta
     1081 ctcatctttt cacctttatt tcttagttaa aaaattatat cacaaaaaac tataaatata
     1141 tagttaaaaa tgcagtataa caatattcat aattatcgct tgggtgtaat tttctttact
     1201 aacgcatttt aacacataaa aaaataaatc gactataagg tcgattttat attgattatg
     1261 ccgcagaagg gacttgaacc cccacggtgt tgccaccaac ggattttgag tccgtcacgt
     1321 ctgccattcc atcactgcgg caaaatgcta ctttaagata gtagcataaa caaaagtaaa
     1381 atgcaagtat tttttgcaat tcatatttcg ctgtattcta tttctgcgat ttcaggctcg
     1441 cagcccattt ctacccttga tattctgtcg aacttccttt caattttgtt ggaagttatg
     1501 ttaatcttat ccacatcatc tgaaacacgt ttaatatttc tagataaaga ctcccatctg
     1561 tccttgtaga gcttaaattc ttttcctaat ttattaagct cttcgtgtat tattcctgca
     1621 tacttttctc tctcaatatt tcttaaaaca acctgaaccg ttgacaaaac tgacatcaat
     1681 gttgttggag aagttatcca aactttcttt tcgcctgcat aatccaacaa gtcctgatga
     1741 tatgcgttta tttccgcaaa tattgcttct gccggaataa acattattgc ctggtctgaa
     1801 gtttgtcccg gaattatgta ttttgaagat atatcattaa tatgcatctt tacatttaat
     1861 ttaaagtctc ttgaatattg ttttctttca gcttcagaaa tattgacatc aatcatgcgc
     1921 tggtaatttt caagaggaaa tttcgagtct atgcaaagca ttcctaccgg ctccggtatg
     1981 tataaaacag catcggcaat cattgaattt tctaattttt tttggatttc atatatttta
     2041 tcgttctttt ccccaaagac cacagacaaa atgtggttaa gctgcacctc tccaaaagtg
     2101 cctctgcttt ttttatcggt taaaacatcc tgcaaggaaa ctatatttgt ggaaaggctg
     2161 tctattttct tttgtgcttc atctattctt gacaatcttt caagtatgtt gacaaatgtt
     2221 ttgttggttt tctcaaaccc ttcgttcaat ctttcttcaa ctcttaagtt tatcctgtca
     2281 agattttggc ttactctttc attcaaagat ataaaatttt catttagagt tttagatgta
     2341 tcagcgctga attttgcgaa tgattctgtc agcttattgg agtttctatc tattctttca
     2401 actgtttcca gcttgtttgt caacaattcc ctcagctgct tctgctcaga ctcagcgatt
     2461 tttcgattta ctgaatcaat taaatccaaa ttattatcct ttaaataaat tttaagctct
     2521 gatattatgc gctcgtttgt attgtttttt atttgtttta taagtaaaaa aatattgatt
     2581 atcaatgata aagaaagcaa tgatataata actgaatcgt acatggacac ctccccttta
     2641 cttattaata atactacaaa atcgaacatt tgtccactta aattactctc gtatgcttac
     2701 aatcttatag attacttcat aaaaactcta aaggattttt tggaggtata ttaaattttt
     2761 ggaagagtga taatataagt gcatccttta tttaaatcag tttttaattc catgtctccg
     2821 ccatgtgact taattatttt atatgctatt gacagcccta agcccgttcc tcccgtctga
     2881 gaattacgag agtcatcaat cctgacaaag ggtttgaaaa tattttttgc aagatgtgac
     2941 ggaattccta taccatcatc actgaaataa attagtacat ttgttttatc atcatataaa
     3001 ctt
//
LOCUS       DAOSOW010000236         2994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_32953_length_2994_cov_2.434842, whole genome shotgun sequence.
ACCESSION   DAOSOW010000236 DAOSOW010000000
VERSION     DAOSOW010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32953_length_2994_cov_2.434842"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1712
                     /locus_tag="PLD67_06585"
     CDS             93..1712
                     /locus_tag="PLD67_06585"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HOT22221.1"
                     /translation="MKYNLIFGHKTPDTDSVCSAIALSHLKNKLNEPSKAYILGDINK
                     ETAFVLKYFNVSVPEILDNVKIQIKDLDFERVKPFTKNKSVHFAYFHMNENKLRTLPI
                     VDEENNLCGLITMKDIAMSLINTDQHYLCTSYDNIIETLLGKSILKFDEIIKGYVIVA
                     AFEEKTLRKSDIVNENSIVITGDRYDVIKYAIESKVRLIIVTGDLKVTEELIEMAIKN
                     KVNIIGTAYHTYYTAKNISLAKYVDDIMKKSDIMMFSEEDYLNDCKEDIEQSKHSKFP
                     IISKDKKYLGLLSRSHLINPKKKKVILVDHNEMNQSADGIEEAEILEVIDHHKIGDIK
                     TSIPISFRNTPVGSTSTIIFQMYRENNVELSKDIAGLILSGIISDTLLFKSPTTTGRD
                     KYAVNELLKIVDLDLHEYAMDMFRAGTSLEGKTLEEIIYQDFKKFNLVYKNVGISQVF
                     TLDINEIMNRKEEFVKLIDDITSDKYYLIIMAVTDIVNEGSYIFYTSSKEKLVRMIFE
                     EDVHQGIYIDKCISRKKQIVPKVINALNQLK"
     gene            complement(1741..2280)
                     /locus_tag="PLD67_06590"
     CDS             complement(1741..2280)
                     /locus_tag="PLD67_06590"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HOT22222.1"
                     /translation="MHYNTMDLLLFFLIYGFLGFLLETVFRSIADRKVYISRGFLTNG
                     FCPLYGICALLIIEGFILCELALSNPLTAMIVATAGSIFLVTLIEYIVGRMLDRVFHY
                     KLWDYSAYRLNLHSYICLEFSLLWGIMALLLVYFMHPVMEVLVFAIPYNTRAVAVVLF
                     LSMLIINASYQIRKRVYGG"
     gene            complement(2405..>2994)
                     /locus_tag="PLD67_06595"
     CDS             complement(2405..>2994)
                     /locus_tag="PLD67_06595"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOT22223.1"
                     /translation="LSQVIHFTTISGVLVVAVFLSFPNEIAMAVYNNRQVGLMIKLIS
                     YVCPFMYLNMVISSMLNALGEQVSSFVVNIIESVLKIFIIYVFVPVYGFNAYLFALFI
                     TTILNTILYLFRLLQISSIVFDISDWIFKPIVAAVIAGILSRYSYFLFVQNSRHIISL
                     ISSICILCLLYLMGLIAFKSVRLESINDLKKYVLK"
BASE COUNT         1126 a          474 c          513 g          881 t
ORIGIN      
        1 tagttacggg tacggttctt ttagtcctcg atatttgggg gacgattcct ttcactctga
       61 aactcactaa tattacgtaa atgaggtatt ttatgaaata taatttaatt ttcgggcata
      121 aaaccccgga cactgacagc gtatgttctg ctatcgctct tagtcactta aaaaataagc
      181 tcaatgaacc ttcaaaagca tatatattag gtgatattaa caaggaaaca gcctttgttt
      241 taaaatattt caatgtaagt gtcccggaaa ttctggataa tgtaaaaata caaataaagg
      301 atttagattt cgaaagagta aagcccttta ccaagaataa atcagtacac ttcgcttatt
      361 ttcacatgaa tgaaaacaaa ctaaggactc ttcccatagt tgatgaagaa aataatttgt
      421 gcgggctgat aacaatgaag gatattgcca tgagtttgat aaatacagat cagcattatc
      481 tatgcacatc ttatgataat ataattgaaa cactccttgg aaaaagtatt ttgaaatttg
      541 atgaaataat caaaggttat gtaattgttg ctgcctttga ggaaaaaaca cttcgcaaat
      601 cagacattgt aaatgaaaat tcaattgtaa tcacaggcga cagatatgat gtgataaagt
      661 atgccattga atcaaaagta agactcataa ttgtcacggg ggatttgaag gtaactgaag
      721 agcttattga aatggcaatt aaaaataagg ttaatataat aggcactgct tatcatacat
      781 attatacagc aaagaatata tctcttgcta aatatgtgga tgatattatg aagaagagtg
      841 atattatgat gttttcagag gaagattatc tgaatgattg caaggaggat attgagcagt
      901 cgaagcattc aaaattcccc ataatatcaa aggataaaaa atatctgggg cttcttagca
      961 gaagccattt aatcaatccg aagaaaaaaa aggtaatctt ggttgaccat aatgaaatga
     1021 atcaaagtgc cgatgggatt gaagaagccg aaatattgga agttatagac caccataaaa
     1081 tcggggatat taagacttct attccgatca gtttcagaaa tacacctgtt ggaagcacaa
     1141 gcaccataat ttttcaaatg tacagggaga acaatgtaga attatcaaag gatatagccg
     1201 ggctcatact gtcgggaata atctccgata cgctgctttt taagtctcct accacaaccg
     1261 gaagagacaa atatgcagta aacgagctgc taaaaattgt tgatttagat ttacatgaat
     1321 atgcaatgga tatgtttagg gcaggaacat ccttggaagg aaaaacattg gaagaaataa
     1381 tttatcagga ttttaaaaaa tttaacttgg tttataagaa tgtgggtata tcccaggtat
     1441 tcaccttgga tattaatgaa ataatgaaca ggaaggaaga gtttgtaaag cttatcgacg
     1501 atattacctc tgataaatat tacttaataa taatggcagt aacggatatt gtaaacgagg
     1561 gctcttatat tttttatact tcatcaaagg aaaagttggt aagaatgatt tttgaagaag
     1621 atgtacacca aggtatctat atagataaat gcatctcaag aaaaaaacaa atagttccca
     1681 aagtaataaa cgccctaaac cagttaaaat aatttcaccg gattaattcc ttaaagggtc
     1741 tcatcctcca tagaccctct ttcttatttg gtaggatgca ttaataatta acattgataa
     1801 aaataaaact acagcaactg ccctagtatt atatggtatt gcaaacacca gtacctccat
     1861 cacgggatgc ataaaatata ccaacaaaag tgccataatt ccccaaagca atgaaaattc
     1921 aaggcatata tatgaatgca ggtttaacct gtatgcactg taatcccata atttataatg
     1981 aaataccctg tcgagcattc taccaactat atattcaatt agggtgacca aaaaaatact
     2041 tcccgctgtc gcaactatca ttgccgttaa ggggttgctt agcgctaatt cgcacaatat
     2101 aaagccctca attatcaata atgcgcagat tccatataag ggacaaaatc cattagtaag
     2161 aaatcctctg cttatataaa cctttctgtc cgcaatactt ctaaagactg tttcaagcaa
     2221 gaatcccaag aatccgtata ttaaaaaaaa caacaataaa tccatagtat tataatgcat
     2281 tttctcactc cttgaaatgt attctttgga gtaatttaag aaatatgcta ttataagaat
     2341 gccaatttat gttttgaatt attgaaattt ccagttttat gacaagggga cgggggtgtt
     2401 gtcatcattt taaaacatat ttttttaaat catttatgga ttcaagtcgt acacttttaa
     2461 aagcaatcag tcccattaag tataagaggc ataatatgca tattgacgat attaaggaaa
     2521 taatatgacg ggaattttgc acgaacaaaa aatatgaata tctagataat attccggcaa
     2581 ttactgcagc aacaatgggt ttaaaaatcc agtcggaaat atcaaaaaca atgcttgaaa
     2641 tctgcaataa tctaaacagg tataaaatcg tattaagtat tgtagttata aacaatgcaa
     2701 acaaatatgc attgaatccg taaacaggca caaatacgta tatgataaaa attttcaaca
     2761 cagattcaat aatatttaca acaaaagagc ttacctgctc tccaagagca tttagcatgg
     2821 atgatataac catgtttaaa tacatgaaag gacacacata tgatattaat tttatcatga
     2881 gacccacttg gcggttgttg taaacagcca tggctatttc attggggaat gataaaaata
     2941 ccgctaccac caatactcca gaaatagttg taaagtggat tacctgagac agtg
//
LOCUS       DAOSOW010000237         2977 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_33183_length_2977_cov_2.457906, whole genome shotgun sequence.
ACCESSION   DAOSOW010000237 DAOSOW010000000
VERSION     DAOSOW010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33183_length_2977_cov_2.457906"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="PLD67_06600"
     CDS             <1..1142
                     /locus_tag="PLD67_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22224.1"
                     /translation="NNNKVIKITMSLTLFLGCVAYLAAQIKAGSELFAHLMGWDPLLT
                     GFVIFGVLALYMAFSGEVGGLLTQAFQGLVMIVAGAVIIAAFFNITGGFGEVLRVTAE
                     AEAVTVNNITKEFSPDMFNAWGSMPRVVAMSWMLIPILGTVGQPQVITRMYALKDPRD
                     MPKMGMYTALSHTMVGLLTLVVANGALYLVATGKIDPLVSGDRAIYALADYSGVVAQL
                     FVYAGVLAAAMSSASLFLSICSTVVSRDIPNALGFKFEPKKQVQISRISMLVLGIASI
                     IFSITQGEMVGILGTFGWGTLMSATFPVFVIGLLWRRANEKGVMCGSVVGLALNIISF
                     AGFKWPGALPWYFNVVALSIAVTVFVSLVTKEQEINKKLEAVIEL"
     gene            1203..2768
                     /locus_tag="PLD67_06605"
     CDS             1203..2768
                     /locus_tag="PLD67_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOT22225.1"
                     /translation="MMENKGWIGNYSYFRARINGNSEAVYDLDNNIRYTYNDLDERAN
                     ILANYLKEKLGIKEGDRIAFISRNRIEMIDAYYATAKLGAVLIPYNVRLSSVEFEKLL
                     NNETPKILFYEVAFKDLVSQFRSKVNIESYIVLNDKDNYGEDIPYSDVINYENKNMIY
                     CSTLKLDDIHLIIHTGGTTGLPKGAMISHQSLMFNSFNEICTWGLNYQDSAFIILPLY
                     HTGGWNLLTLPLLHCGGRIIINKQFDPRLTLEVIEKEKTTLLFGAATIFRMISDLPEF
                     EKADLSSLKWVMAGAAPTPINIMERYWNKGLKFILGYGMTEAGPNNLSSTPQFMTDQM
                     IRDKYASVGKPMYMTMVKVVDEKGESVGVNEVGELLWSGPQIFSGYWNHEIETNKTLA
                     DGWVYTGDMAKVDEDGYYYIVGRKKNMYISGGENVFPPEVESAIYEIPQVQEVCVFGV
                     PDEKWGEVGKAVISLKKGQSITKEEIIKILRNKLAGYKIPKYIQFIDDIPKNNVGKIV
                     VSKIMELYGECRD"
     gene            2780..>2977
                     /locus_tag="PLD67_06610"
     CDS             2780..>2977
                     /locus_tag="PLD67_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22226.1"
                     /translation="MSIVGIRNIGIYMPPKIKDSAKISELSGIPEEIIKEKFGILHVH
                     EANDEETVSYMGIEASKRALNG"
BASE COUNT         1032 a          397 c          649 g          899 t
ORIGIN      
        1 ataataataa caaggttatc aaaattacta tgtctctaac gttatttttg ggttgtgttg
       61 cttatttagc ggcccaaata aaagcaggct ccgaattgtt tgcacattta atgggttggg
      121 atcccttgtt gaccggcttt gttatatttg gtgtattggc tttatatatg gcattcagcg
      181 gagaagtagg cggattgttg actcaagcat ttcaaggatt agtaatgata gtagcaggag
      241 ctgtaatcat cgcagcgttt tttaacataa caggcggatt tggagaggtt ttaagggtta
      301 cagccgaagc ggaagcagta acggttaata atattacaaa agaatttagt cctgacatgt
      361 ttaatgcctg gggaagtatg ccaagagtag ttgcaatgtc ttggatgctc ataccgatac
      421 tcggtactgt cggacaacca caggttataa caagaatgta cgctttaaaa gaccctaggg
      481 atatgcctaa gatgggtatg tatacagctt tgtctcatac aatggtgggt ttgctcacat
      541 tggtagtagc aaatggagca ttgtacttag tagctaccgg aaaaatagat ccactggtaa
      601 gcggggacag agcaatatat gctttagcag attattccgg tgttgtagct cagttatttg
      661 tatatgcagg agttttagct gctgcaatgt catctgcaag cttgttttta tcaatatgct
      721 caacagttgt ttcgagagat attccaaatg ctttagggtt taagtttgag cctaaaaaac
      781 aagttcaaat atcaagaatt tcgatgcttg tacttggaat tgcttcaata atattttcaa
      841 taactcaggg cgaaatggtt ggtattttgg gaacatttgg ctggggtaca ttaatgtctg
      901 ccactttccc cgtgtttgtt ataggtttgt tatggcgcag agcaaatgaa aaaggcgtaa
      961 tgtgcggctc ggtagttgga ttggcattaa atataatttc atttgccgga tttaaatggc
     1021 ctggcgcact tccttggtat ttcaatgttg tggcattaag tatagcagta actgtttttg
     1081 tttcattagt aacaaaagaa caagagataa ataaaaaatt ggaagctgtt attgaattat
     1141 aaaattttga ggcagtattt agattttaaa tactgcctca aacctaaatt atatggaggt
     1201 gcatgatgga aaacaaaggt tggataggca attattcata ttttagagct agaataaatg
     1261 gaaacagtga agcagtatat gatttagata ataatattcg ctatacatat aatgacttgg
     1321 atgaaagagc taatatcctg gcaaattatt taaaagaaaa gctcggtata aaagaaggag
     1381 atagaatagc attcatctct agaaatagaa ttgaaatgat tgatgcctat tatgcaacag
     1441 caaagttagg agcggtattg ataccttaca atgtaaggtt atcttccgtt gaatttgaaa
     1501 aactattaaa taatgaaaca cctaaaattc tattttatga agttgcattt aaggatttag
     1561 tatctcaatt taggagcaag gttaatatag aaagctatat tgttcttaat gataaagata
     1621 attacggcga agatattcca tacagtgacg ttataaatta tgagaataaa aacatgattt
     1681 attgcagtac tcttaaattg gatgatattc acttaataat acatacagga ggaacaaccg
     1741 gacttccaaa gggagcaatg atttctcatc aatcattgat gtttaattcc tttaatgaaa
     1801 tatgcacctg ggggttgaat tatcaggaca gcgcttttat catacttccc ttatatcaca
     1861 caggaggatg gaatttactt acattaccgt tattgcactg cggaggcaga atcataatta
     1921 ataaacaatt tgacccaaga ctaacattgg aagttataga aaaagagaaa actacattac
     1981 tttttggagc tgcaacaata tttagaatga tttctgattt acctgaattt gaaaaagctg
     2041 atttatcaag cttaaaatgg gttatggcag gcgctgctcc tacaccaatc aatataatgg
     2101 aaagatattg gaataaagga ctgaaattta ttttaggata cggaatgaca gaagccggcc
     2161 ctaataattt gtcaagtacg ccccagttta tgacagacca gatgattaga gataaatatg
     2221 catcagttgg aaaaccaatg tatatgacta tggttaaagt tgtagatgaa aaaggtgaat
     2281 ccgtaggggt aaatgaagta ggagaattgt tatggagcgg tccgcaaatt ttctccggat
     2341 attggaatca tgaaattgaa acaaataaaa ctcttgcgga tggatgggta tatacaggcg
     2401 atatggcaaa ggttgatgaa gatgggtatt actatatagt tggaagaaag aaaaatatgt
     2461 atataagcgg gggagaaaat gtatttcctc cggaagtaga aagtgcaatt tatgaaatac
     2521 cccaggtaca agaagtatgt gtatttggcg tacctgatga gaaatggggg gaagtcggaa
     2581 aggctgtaat atctttgaaa aagggccaaa gtattacaaa agaagaaata ataaaaattc
     2641 tacgaaataa gcttgcagga tataaaattc caaaatatat tcaatttata gacgatattc
     2701 cgaaaaataa cgtcggtaaa attgttgtat ctaaaattat ggagttgtat ggagaatgta
     2761 gagactaagg agggtattta tgagcatcgt aggaatcaga aatattggta tatatatgcc
     2821 tcctaaaata aaggacagcg ctaaaatatc agaattatca ggaatacctg aagaaataat
     2881 aaaggaaaag tttggtatat tgcatgtaca tgaagctaat gacgaagaaa cagtgtcata
     2941 tatggggatt gaagcatcaa aaagggcatt gaatggt
//
LOCUS       DAOSOW010000238         2941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_33707_length_2941_cov_2.940748, whole genome shotgun sequence.
ACCESSION   DAOSOW010000238 DAOSOW010000000
VERSION     DAOSOW010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33707_length_2941_cov_2.940748"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..721
                     /locus_tag="PLD67_06615"
     CDS             35..721
                     /locus_tag="PLD67_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008879418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOT22227.1"
                     /translation="MKKKILSLTLALIIALSSTSAFAAEQVYYTNFNIYDLCKNLGYY
                     YFEPVNTNNNIGIKTNIEKAPVNNGAETTQAPVNNGAETAQAPSNERATTEAATTAAP
                     SVSNYERRVVELVNVERQKNGLAPLTLDPAISNVARIKSKDMSDNNYFAHQSPTYGSA
                     GDMLSQHGIRYSAWGENIAAGQRTPEEVVSAWMNSPGHRANILGSHYTKIGVGYVNNS
                     RPYWTQMFTN"
     gene            complement(886..1614)
                     /locus_tag="PLD67_06620"
     CDS             complement(886..1614)
                     /locus_tag="PLD67_06620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HOT22228.1"
                     /translation="MDYLYDGTFEGLMTCIYYHYKKEKATGIYEISEYQQSIVYEAEI
                     VKTDISKAKIVCDSINRQISKEAYIHVYYCYLSNDSKKENLILDFLIFAFKYGRDTMN
                     FYTHEKVLPINEIYKKVAREEHRVLGILRFSDIGGILFAKYSPDNDISILLADHFADR
                     YKHEKFIIYDEKRKKAVVYANNSWEIKENINIDNVEFSLDETKIHKLWKQYFTDLAIK
                     ERKNINLQFQFVPARYRKNMTEFK"
     gene            complement(1617..2864)
                     /locus_tag="PLD67_06625"
     CDS             complement(1617..2864)
                     /locus_tag="PLD67_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015312295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HOT22229.1"
                     /translation="MEDNLFEKLRILSDAAKYDVSCVSSGVERKNNTVGGIGNASAAG
                     ICHAWSTDGRCISLLKILLTNDCIYDCKYCLNRVTNPIKRATFTPEEVAELTIQFYRR
                     NYIEGLFLSSAVEKSPDHTMENIYRVLELLRYKYNFWGYVHVKVIPGANPLLVQKTGM
                     LADRMSVNIELPTRESLSLLAPQKKMENILKPMSLIHNEISRAIEDKSHFKNAQKFVP
                     GGQSTQMIIGATPDSDKTILSASQNLYDKFRMKRVFFSAYVPIMSSPNLPAVSTMPPL
                     LREHRLYQADWLMRFYYYKSEELFTEVEQNLDLEFDPKMVYALRNIDKFPIEINNASY
                     EELLRIPGLGVTSAQRIIRERKNAQISYDSLKKMRTVLKRAKYFITVKGKYYGNTKID
                     PEAIKAEIRSKEIQRQISIFELL"
BASE COUNT          933 a          555 c          453 g         1000 t
ORIGIN      
        1 aatcttatta ttaataattt catattttta cgaaatgaaa aagaaaattt tatctttaac
       61 attagcgcta attatagcac tatcttcaac atctgcattt gctgcagaac aagtgtatta
      121 tacaaacttt aatatttatg acttgtgtaa aaatttaggt tattattatt ttgaacctgt
      181 taatacaaac aataacattg gaataaaaac aaatattgaa aaagctcctg taaataacgg
      241 agccgaaaca actcaagctc ctgtaaataa cggagccgaa acagctcaag ctccttcaaa
      301 tgaaagagca acaacagaag ctgcaacaac tgctgctcct tcagtatcaa attatgaaag
      361 aagagtagta gagcttgtaa atgttgaaag acagaaaaac ggattagcac cgcttacttt
      421 ggatcctgca atatcaaatg ttgcaagaat caaatcaaaa gatatgtcag acaacaatta
      481 ttttgcacac caatctccaa cttacggtag tgccggtgat atgctttctc agcacggaat
      541 cagatacagt gcatggggtg aaaatatagc agcaggccag agaacaccgg aagaagtagt
      601 aagtgcatgg atgaattcac cgggccacag agcaaatata cttggatcac actatacaaa
      661 aataggtgtt ggctatgtga ataacagtag accatattgg acacaaatgt ttacaaatta
      721 agcaaatgac ccaaatcttt gatttggtgt cagttgctta tcctgagcgt atgcgaagga
      781 tcccatgtct taaaagatag attcttcact gcgttcagaa tgacaaagaa aaatatactc
      841 taaatcaata acagcaggca tttctaattg aagtgtctgc tgtttttatt tgaattcagt
      901 catatttttt ctgtatcttg cgggtacaaa ttgaaactgt aagttaatat tttttctctc
      961 cttaattgcc aaatcggtaa agtattgttt ccacaattta tgtatctttg tttcatctaa
     1021 agaaaattca acattatcaa tatttatgtt ttctttaatt tcccaactgt tattagcgta
     1081 cacaaccgct ttttttcttt tttcatcata tataataaac ttttcatgct tatatctatc
     1141 tgcaaaatgg tcagccaata agatgcttat gtcattatcg ggagaatatt tagcaaacaa
     1201 aattcctcct atatcagaaa acctgagtat gccaagcacc ctgtgctctt ctcttgctac
     1261 ctttttgtat atttcattaa taggaaggac cttttcatgt gtatagaagt tcatggtatc
     1321 tctgccgtac ttaaatgcaa atattaaaaa atctaaaatc aagttttcct ttttagaatc
     1381 atttgaaaga tagcaataat aaacatgtat ataagcctct ttagaaatct gcctattgat
     1441 tgaatcgcac actatttttg ctttcgaaat atctgttttt acaatttccg cctcatatac
     1501 aattgattgc tgatattcag atatttcata tataccggtc gccttttctt ttttataatg
     1561 ataataaatg catgtcatca atccttcaaa tgtaccgtcg tataaataat ccatgattat
     1621 aacaattcaa agatactaat ttgtctttga atttccttac tccttatttc tgcttttatt
     1681 gcttccgggt caatctttgt attgccatag tatttacctt taacggtaat aaaatattta
     1741 gctctcttta aaacagtcct cattttttta agagaatcat aggaaatttg ggcatttttt
     1801 ctttccctta ttatcctctg ggcactggtt accccaagcc ccggtatcct taaaagctct
     1861 tcgtatgatg cattgttgat ttctattgga aatttgtcaa tatttctcaa tgcatacacc
     1921 attttcgggt caaattccaa atcaagattt tgctcaactt cagtaaacaa ttcctcagat
     1981 ttataataat aaaaccgcat gagccagtct gcctgataca acctgtgctc ccttaataat
     2041 ggaggcatgg tacttacggc aggcaaattc ggtgatgaca taatcggaac gtatgcagaa
     2101 aaaaacactc ttttcatcct gaatttatca tacaaatttt gtgatgcgct taggattgtt
     2161 ttatcgctgt caggggtggc tccgattatc atctgtgtcg attgaccgcc cggtacaaac
     2221 ttctgagcat ttttaaaatg actcttatct tcaatagcac gagaaatttc attatgtatg
     2281 agagacattg gcttaagaat attctccatc tttttttgag gtgccaataa ggataagctt
     2341 tctcttgtgg gaagctcgat attaacgctc atcctgtctg ccagcatacc tgttttttga
     2401 actaatagag gatttgctcc cggaataacc ttcacatgaa catagcccca aaaattatac
     2461 ttatatctta aaagctcaag taccctgtaa atattctcca tggtatggtc gggacttttt
     2521 tcaacggctg aacttaaaaa taacccttca atataatttc ttctgtaaaa ttgaatagtt
     2581 agctccgcaa cctcttccgg ggtaaaagtt gctcttttga ttggatttgt caccctgttc
     2641 aaacaatatt tgcaatcata aatgcagtcg tttgtaagta aaatttttag aagtgaaata
     2701 catctgccat ccgtggacca ggcatgacaa atgcctgcgg cagaagcatt gcctattccg
     2761 cccactgtat tgttttttct ttctacaccg cttgaaacac atgatacgtc gtattttgcc
     2821 gcatcagaca aaatccttaa cttttcaaac aaattgtctt ccatattttc accaagtttt
     2881 tcttagtctt ctttgattat tatattataa agaaaaacat ttgtcaaaca tttgttcgat
     2941 t
//
LOCUS       DAOSOW010000239         2920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_34016_length_2920_cov_3.735079, whole genome shotgun sequence.
ACCESSION   DAOSOW010000239 DAOSOW010000000
VERSION     DAOSOW010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34016_length_2920_cov_3.735079"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..1857
                     /locus_tag="PLD67_06630"
     CDS             40..1857
                     /locus_tag="PLD67_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009015136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOT22230.1"
                     /translation="MKRLVADQMVEYLERRNVEYIFGLCGHTVIGFLDALSRSEKLKY
                     VSFRNEQLAAMAADGYARIKKKAGVVLCHLGPGLANTVTGVANASFDSIPMVVIAGDV
                     PRYYYGKHPHQEVNMHADASQYEILRPIVKRAWRIDDAEALPEILDKAFRLAESGRPG
                     PVLISIPMDVFSVKIDEELFNRTFKDSSETIKPALAPSAAKAIAKKLIEAKNPVIHAG
                     GGILLAGASEELRQLVEFLDIPVSRTLMGQGCLSDTHPLMLGQTGFWGLEFTHKFTAN
                     ADVILGLGTRFAEADSSSWYTGVTFDMNKTEFLQIDIDPAEIGRNYPVSIGAIADLKL
                     ALAQILEEAKALVPEGIKRPGLRDMIKANRDEFRKSNIEVSTDGRFPMTPQRILRDVK
                     SVIPEDAVIFTDVGWNKNGVAQQFDITIPGTIHHSSGLATMGFGGSALIGGKLAAPDK
                     VVLTLVGDGGFGVNPGALATAVEQNIPVVWVVMNNSAFGTIAGLEYSNYQTKFGTVFT
                     TPDGKPYSPNWAEVAKAYGVDAVKIKSADDFKPELEKAIKSNKPYLLDVPMENIAVPT
                     PGSWNINDIYNPKEGVVNGKLVKNEKGEYMPPDHSTSHK"
     gene            1879..2724
                     /locus_tag="PLD67_06635"
     CDS             1879..2724
                     /locus_tag="PLD67_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HOT22231.1"
                     /translation="MKRKFSLAYLTIPGTHPVDQIRIAAEAGYDFVSLRPIPMHLPNE
                     PLFQFDLDRKLFKDIRDALEQNDIKLMDIELARVREDLKVEDYESAFAAAAELGATDV
                     LSSIWTKDKEFAFKEFAKICDLAAKYNLKVNLEFVTFSGVTDLTGALEVLDKVGRPNA
                     YLMVDTLHAHRSRVSPEDLAKVDRKKFGFIHLCDGPGEIPSLEDPSMIGVAREGRLYA
                     GEGEIDLKGMLLAMPDNPISIELPNSKEMKERGAAGHATRCLITAKELLVNMAKEEDI
                     ECQSI"
     gene            2709..>2920
                     /locus_tag="PLD67_06640"
     CDS             2709..>2920
                     /locus_tag="PLD67_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOT22232.1"
                     /translation="MPVNIKTKMVTLLGKPLSQSYAARMQNAAYRAAGIDMLYFYTEV
                     ENDHLPDVVNGIRYMNFAGFAVTKPN"
BASE COUNT          967 a          474 c          694 g          785 t
ORIGIN      
        1 gagcctgaaa gagaaggaaa ataattaagg aggtaatata tgaaaagatt agttgcagac
       61 caaatggtag aatatttgga aagacgtaat gtagagtaca tttttggttt atgcggtcat
      121 acggtaattg gttttttgga cgcattatcg agaagcgaaa aactaaagta tgtatcattt
      181 aggaatgagc agcttgctgc tatggctgcc gacggttatg caagaatcaa aaagaaggcc
      241 ggagtagtac tatgccattt aggcccgggg ttggctaata ccgtaaccgg agttgccaat
      301 gcttcgtttg attcaatacc catggttgtt attgcaggag atgttccaag atattattat
      361 ggaaaacatc ctcatcaaga agttaatatg catgcagatg cttctcaata cgaaatacta
      421 agaccaattg taaaaagagc ttggagaatt gacgatgcgg aagcactgcc tgaaattttg
      481 gataaggctt tcaggcttgc agaaagcggg agaccgggac cggtgcttat atcaatcccc
      541 atggatgtat tttctgttaa aatagatgaa gaattattta accgtacatt taaagacagc
      601 tcggaaacaa taaaaccggc actggcacct tcagcagcaa aggcaatagc taaaaaactt
      661 atagaagcca aaaatcctgt tattcatgca ggaggaggaa tacttttggc aggtgcatca
      721 gaggaattaa gacagctggt agaatttttg gacattcctg tttcaagaac acttatgggg
      781 caaggatgtt tgtcggatac ccatcccttg atgcttggtc aaacaggatt ctggggtctt
      841 gagtttacac acaaatttac tgcaaatgca gatgtaatac ttggattagg aacacgattc
      901 gccgaagcag acagttcaag ctggtatacg ggtgttactt ttgatatgaa taaaacagaa
      961 tttttgcaga tagatattga cccggcagaa ataggaagaa attatcccgt atcaatcggt
     1021 gcaatagcgg atttaaaact tgccttggct caaattcttg aagaagccaa agcccttgta
     1081 ccggaaggga ttaaaagacc gggtttaagg gatatgatta aagcaaatcg tgatgagttt
     1141 agaaagtcaa atattgaagt ttcaacagat ggcagattcc caatgacacc tcaaagaatt
     1201 ttaagagatg ttaaatcggt tataccggaa gatgcggtta tctttacgga tgtaggctgg
     1261 aataaaaacg gagttgctca gcaatttgat attactatac cgggaacaat ccaccacagt
     1321 tcagggttgg caactatggg atttggcggc tcggcgctta taggcggcaa gcttgcagca
     1381 ccggataagg ttgtattaac acttgtagga gacgggggat tcggagttaa tcccggagca
     1441 ttggcaactg cagtagagca aaacattccg gtagtgtggg tagtaatgaa caattctgca
     1501 tttggaacaa ttgcgggatt agaatattca aattatcaaa caaaattcgg aacagtattc
     1561 acaacacctg acggtaaacc ttattcgcca aattgggcag aggttgccaa agcttatgga
     1621 gttgatgctg taaaaataaa atcagctgat gatttcaaac cggaacttga gaaggcaatc
     1681 aagagcaaca agccatattt gttggatgta ccgatggaaa atattgcagt gcccacaccg
     1741 ggaagctgga atataaatga tatttataat cctaaggaag gtgttgttaa cggtaaattg
     1801 gtaaagaatg aaaaagggga atatatgcct ccagatcatt cgacatcaca taaataagtg
     1861 ttaccaaagg aggataatat gaaaagaaag ttttcgctgg cttatttgac cataccgggt
     1921 actcatccgg ttgatcagat aagaattgcc gctgaggcag gatatgattt tgtgagttta
     1981 agaccaattc ctatgcatct tccaaatgag ccattgtttc aatttgattt ggacaggaaa
     2041 ttgttcaagg atataagaga tgcattggag caaaatgata taaagcttat ggatattgag
     2101 ctcgcgcgag ttagagaaga tttaaaagtt gaagattatg aatcagcatt tgctgcagca
     2161 gccgagctag gtgcaacaga tgtgctctca agtatctgga ctaaggataa ggaattcgct
     2221 tttaaagaat ttgcaaaaat ttgcgatttg gctgctaaat ataatttaaa agttaatctt
     2281 gaatttgtaa cattctccgg agtaaccgat cttacggggg ctttagaagt tcttgacaaa
     2341 gtaggtagac ctaatgcata cttaatggta gacactctgc atgctcatag gtctagggtt
     2401 agtcctgagg atttagctaa agttgacaga aagaagtttg gatttattca tctttgtgac
     2461 ggtcccggtg aaattccgtc cttagaagac ccttccatga ttggtgttgc cagagaagga
     2521 agactatatg cgggagaagg agaaattgac cttaagggta tgcttttagc catgcccgat
     2581 aatccgatat caatagaact gccaaattct aaggaaatga aagaaagagg agcagcaggg
     2641 catgctacaa ggtgtctcat tacggcaaaa gaattattag ttaatatggc taaagaggag
     2701 gatatagaat gccagtcaat ataaaaacga aaatggttac attgctggga aaacctttat
     2761 cccagtccta tgctgcaagg atgcaaaatg cagcctatag agctgccgga attgatatgt
     2821 tgtatttcta taccgaagta gaaaatgacc acctgcctga tgtggtaaat ggaataaggt
     2881 atatgaattt tgcaggtttt gcggttacaa aaccaaacaa
//
LOCUS       DAOSOW010000240         2920 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_34019_length_2920_cov_3.431065, whole genome shotgun sequence.
ACCESSION   DAOSOW010000240 DAOSOW010000000
VERSION     DAOSOW010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34019_length_2920_cov_3.431065"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            231..371
                     /locus_tag="PLD67_06645"
     CDS             231..371
                     /locus_tag="PLD67_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22233.1"
                     /translation="MEGSKLFWSSGTWIFFLVQALVVALAVYLYWYLGKKEKEACEEK
                     KE"
     gene            401..1762
                     /locus_tag="PLD67_06650"
     CDS             401..1762
                     /locus_tag="PLD67_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HOT22234.1"
                     /translation="MKLNVIIPFVSYLLIMLGIGMYAMRRIKTYDDFIIGGRSIGAWV
                     SSFALITSYASGYTYTAAPGMSYTGGWSTMWWSTGDAPGNALSFGLLGRRLRKFSELL
                     GAITLPEYYEKRFKSPGLRLLTSIIIITTVSLHLVAQWQASGILLSVTFGTDYLVGML
                     IGGAVVLAYTIMGGYLATVYTDFMQGVVMFIGTHILFWSAISKVGGFNALNDQLALIN
                     PGLVTPWGPDGSYFGLIAAASPIILIIMGSFGMPHVTVRHLSLKNPDTARKAMLITAI
                     FVALFSFAYYMVGAAGLILLGPGLENPETSGVLLWFEVLPPVGAGILVSAAVAAIMST
                     ADGFLMLIVSTIAHDILNRFLLPNTPQEKRIRIARIITAIIVILSLVAAINPPAGVFT
                     IVMTVFGGMALAFGIPNIFSVYWKRSTKTGAIACVVLSLTVYVWLTAAGIELFGMSPF
                     IMD"
     gene            1822..2007
                     /locus_tag="PLD67_06655"
     CDS             1822..2007
                     /locus_tag="PLD67_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22235.1"
                     /translation="MEELFDIGTSYGPLPESFVKSSNVSEELKAEALRAEKLMTKNNV
                     LKQSLNINALNEPIPTN"
     gene            2020..2526
                     /locus_tag="PLD67_06660"
     CDS             2020..2526
                     /locus_tag="PLD67_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22236.1"
                     /translation="MDIVCLQTNAEYNSIKNEAADFKDYDCQEIFYPLWLLEVEVCMH
                     LLFSKVKRMSMKVAVDGLTGDISFICKQIETYHAEVKPEQLLPVTAKMDAEGLAKVKD
                     SLLPFVLSKARSFLVRPSINIQNKSIIFKPFYRLSSKDGDDRIIYLDKSNGKIAVLRQ
                     EGKSNDSF"
     gene            2513..>2920
                     /locus_tag="PLD67_06665"
     CDS             2513..>2920
                     /locus_tag="PLD67_06665"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOT22237.1"
                     /translation="MTLFRWDELTWTELTNLRDGIIIIPTGSVEQHGPHLPIGTDTFI
                     AEGFAKKLYNKAIDKKIIIAPSISYTYAKLNSNYLGTINLNGSTLIKLGLDLISEFLR
                     QGFNKIILINGHLESIPFLMEGIELALKAYEKSK"
BASE COUNT          892 a          463 c          629 g          936 t
ORIGIN      
        1 agagtggtac cgcggtgtaa acactgcctc tttataggca gttttttttt gccctttaga
       61 aaccaagatt cttcatatgg ggatgaaagg catgcataaa tattgataat aattccggag
      121 ctttacacat ataggtttat agatagtaat tattttcaga tatatatttc aaatttagtg
      181 tagtaatcgt ggacaaagcc gcaagtttat ttaaagaagg aggtgaaaag atggaaggtt
      241 caaaactttt ttggtcatca ggaacatgga tttttttcct tgttcaggcg ttagtagttg
      301 ctctggcagt ctatttgtat tggtatttgg gaaagaaaga aaaggaagca tgtgaagaaa
      361 aaaaagaata agataaagct aaattaaagg aggcaaaaaa atgaaattaa atgtaataat
      421 accatttgta tcttatctgt taataatgct tggaataggc atgtatgcaa tgcgacgaat
      481 caagacttat gatgatttta ttattggagg aaggtccata ggagcatggg tttcatcctt
      541 tgcacttatt acatcttatg ccagcggtta tacttatact gctgctcccg ggatgtccta
      601 tacgggagga tggtctacca tgtggtggtc aaccggtgat gctcccggaa acgcactatc
      661 atttggatta cttggaagaa ggcttaggaa gttttcggag ttgctgggag ctattacctt
      721 gcctgaatat tacgaaaagc gtttcaaaag cccgggatta agattgttga cttctattat
      781 aattattact actgtaagtc tgcaccttgt agcacaatgg caggcatccg gaatattgct
      841 gagtgtaact tttggaacag attatttagt tggaatgctt ataggcggtg cagtggtact
      901 tgcttataca ataatgggag gatatctggc cactgtttat actgatttta tgcagggagt
      961 ggtgatgttt atcggtactc atattctatt ttggagtgca atttctaaag taggcgggtt
     1021 taacgcatta aatgaccagt tggctttaat aaaccccggt ttggttacac cttggggacc
     1081 tgacggctca tatttcggcc ttattgcagc agcttctcct ataatactta ttataatggg
     1141 gtcatttgga atgcctcatg taaccgtacg ccatttatcc cttaaaaacc ctgatacggc
     1201 aaggaaagca atgcttataa ctgcaatatt tgttgcactc ttttcttttg catattacat
     1261 ggtgggagct gccggattga ttttattggg acccggtttg gaaaaccctg agacatcagg
     1321 tgttttgctt tggtttgaag tgcttccgcc ggttggagcc ggtatccttg tttctgcagc
     1381 tgttgcagct attatgtcta cagcagatgg attcttgatg cttatagttt caaccattgc
     1441 tcatgatata ttaaatagat ttttacttcc aaatacgcct caggagaaaa gaatccgtat
     1501 tgctcgtata attacggcaa tcattgtaat tctttcactt gttgctgcaa taaatcctcc
     1561 ggcaggagtc tttactatag taatgacagt atttggaggc atggctcttg cttttggtat
     1621 tcccaatatc ttctcggttt attggaagcg aagtacgaaa accggtgcaa ttgcttgtgt
     1681 cgttttgtca ttgacggtat atgtatggct tacagctgcc ggaatagagt tgtttggtat
     1741 gagtcccttc ataatggatt aattgttgct atattagcat ttattattgg aagtctgctt
     1801 acacaaaaac caggtcctga gatggaagag ctttttgaca ttggaacttc ttacggaccg
     1861 cttccggaat cttttgtaaa gtcatcaaat gtttcggagg aattaaaagc tgaagctttg
     1921 cgtgcagaaa agcttatgac taaaaacaat gttttaaaac aatcattgaa cataaatgct
     1981 ttgaacgagc cgataccaac taattaggca gtgataacta tggatatagt atgcttacaa
     2041 actaatgcag aatacaattc aataaaaaat gaagcagctg attttaaaga ttatgactgc
     2101 caagagatat tttatccgtt atggttgtta gaggttgaag tttgcatgca tttacttttt
     2161 tcgaaagtaa aaaggatgag tatgaaggta gccgtggatg gattaaccgg tgatataagt
     2221 tttatttgta aacaaataga gacatatcac gcagaagtaa agcctgaaca attactgcct
     2281 gtaaccgcaa aaatggatgc ggaaggattg gcaaaagtga aagattctct gctgcctttt
     2341 gtactatcta aagcacggtc gtttcttgtc agaccttcaa ttaacataca aaacaaaagt
     2401 attattttta agccctttta caggctttct tctaaggatg gggatgacag aatcatttat
     2461 ttagataaga gtaacggtaa aatagccgta ttaagacagg agggaaaaag caatgactct
     2521 ttttagatgg gatgaattaa catggacaga attaaccaat cttcgtgatg gcatcattat
     2581 cataccgaca ggctcggttg aacagcatgg accgcatttg cccattggta cggatacatt
     2641 tatagctgaa ggttttgcta aaaaattgta taataaagct atagataaaa aaataataat
     2701 agctccttct atatcatata cttatgcaaa actaaacagt aattatttag gaacaatcaa
     2761 tttgaacggg agtacactga ttaaactggg cttagacctt atttcggaat tcttgagaca
     2821 gggatttaat aagattattt taataaacgg gcatcttgaa agtatacctt ttctgatgga
     2881 aggaattgag ctggctctta aggcttatga aaaatcaaaa
//
LOCUS       DAOSOW010000241         2901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_34324_length_2901_cov_2.681658, whole genome shotgun sequence.
ACCESSION   DAOSOW010000241 DAOSOW010000000
VERSION     DAOSOW010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34324_length_2901_cov_2.681658"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PLD67_06670"
     CDS             complement(<1..413)
                     /locus_tag="PLD67_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT22238.1"
                     /translation="MEIRRSTINDIDEILKIFEGARKFMKASGNPNQWKDSYPERTLI
                     ESDIENNISYVCVDEGRIVGTFVYFKGIEPDYAEIHDGQWLNEEPYGVVHRIASATGK
                     RGVATYCLNWSFEQCRNLKIDTHHDNIPMHNLLTK"
     gene            719..1672
                     /locus_tag="PLD67_06675"
     CDS             719..1672
                     /locus_tag="PLD67_06675"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOT22239.1"
                     /translation="MTKVKKVVYYICDNHDWGYVSYLVWDILKEEGYLNNKVDFTFDG
                     REVYKYIDNKNNEFYFVPTDIALCRDYPRYLPDMNRYFSDFDMSGMVTWHEGTNALDK
                     VITVHSIGDVNSGCYGPANPLYMHNLMWAMEKLRKEIELDFSVVTEATHWSGVYEGQG
                     DPELLLKYPVPIVDIEIGSTPESWVNMDACRVLALSLLHIFDKDIDDIKLHNLLCVGG
                     VHFDPNFAEAVHTCWNENEFFGVSHIIANQWLVSGEYEGENGIEKASECVDAIIGGID
                     AIVFHDKLKGCYKDVVRALGEKYKVPILKHQALRKPESINW"
     gene            complement(1643..2845)
                     /locus_tag="PLD67_06680"
     CDS             complement(1643..2845)
                     /locus_tag="PLD67_06680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT22240.1"
                     /translation="MLARAFKLIKNLFSTSKTDDCKKEFAIDFCRINLAKLIISAKTL
                     IALGIIQLIVYCIVSKGNLFTKPQIYFGAMNFILLFAMTIFLSVIVKLEKDAAKNVVS
                     IHNAQIFFINFILLWSGGISLLHQLTHGQTIVYIAAVIITAGALYIEPSVSFLIYFTS
                     HLIFSVLLPNFQKSPDLLFGIYLNTTSLVIMSFAISYTRYKRQEQDFKNRKIMQENSD
                     ELKRINKELEEANKKLKIISQYDGLTGIFNRHTFDTKIREEWNRCKRHSMPLSLIMAD
                     IDHFKSFNDKFGHQAGDNCLKLVAEILSECAKRSSDVVARYGGEEFVILLPQTERERA
                     YDLAEKMRKRVEELNITISLGVNTTIPSDESSVREFIKNTDKALYIAKNKRNSTEVYL
                     PIDTFGFS"
BASE COUNT          920 a          520 c          485 g          976 t
ORIGIN      
        1 tttttagtta ataaattatg catgggaata ttgtcatgat gggtatctat ctttaaatta
       61 cggcattgct caaagctcca attcaaacaa taagtagcaa caccccgttt gccggttgct
      121 gaagcaatcc tgtgaacaac tccgtaaggc tcttcattaa gccactggcc gtcgtgtatt
      181 tctgcataat caggctcgat acctttaaaa tatacaaatg ttccgacaat tctaccttca
      241 tcaacacaca cgtaacttat attattttca atatcacttt caatcaatgt tctctcaggg
      301 taactatcct tccactgatt tggatttccg gaagccttca taaattttct tgctccctca
      361 aatattttta aaatttcatc aatatcattt attgtacttc ttctaatttc catattattt
      421 cctttcttta tttcttcaat atcagaatta ttagtcaatt tttgtttcag tttaattata
      481 tatcataact aaaaatatag ccacatacct tctatattta tctcaaaaac cgaagaagca
      541 tcgctaattc catctgaaaa tgaaagagct gtcccgaaga attctcccaa aaagatgaca
      601 gtacccccgt ccccttgtca caaaaagatg acaatacccc cgtccccttg tcacgtcccc
      661 ttgtcattgt gcaaaaaggt ggtataatac taattaaaat aattatagga gtggcataat
      721 gacaaaagtt aaaaaagtcg tatattatat ctgtgacaat catgattggg gatatgtcag
      781 ctacttggtt tgggatatac taaaagaaga aggatactta aacaataagg ttgactttac
      841 ctttgacgga agagaagtct ataaatatat cgataataaa aataatgaat tctattttgt
      901 tcctacagat atagcattat gcagggatta tcccaggtat cttccggata tgaacaggta
      961 tttttctgat tttgatatgt ccggaatggt tacatggcac gaaggtacaa atgcccttga
     1021 caaggttatt accgtacata gtatagggga tgttaattca ggatgttacg gtcctgcaaa
     1081 tcctttatat atgcacaatc ttatgtgggc tatggagaaa ttaagaaaag aaatcgaatt
     1141 ggatttcagc gtagtaactg aagcgaccca ttggtcgggt gtttatgaag ggcaggggga
     1201 ccctgagctt ctcttaaaat atcccgtacc catagtagac atagaaatag gaagtacacc
     1261 ggaaagctgg gtaaatatgg atgcctgcag ggttcttgct ctttctctgc ttcatatatt
     1321 tgacaaagat attgatgata taaagctgca taatcttctt tgcgtgggag gagtacattt
     1381 tgacccgaat tttgcagagg cagtccacac atgttggaat gaaaatgaat tcttcggtgt
     1441 ttctcacata attgcaaatc aatggcttgt ttcaggcgaa tacgaaggag aaaacggaat
     1501 agaaaaagca tctgaatgtg tagatgccat aattggcgga attgatgcaa ttgtttttca
     1561 tgataaatta aaaggatgct ataaggatgt tgtacgcgcc ctgggagaaa aatacaaggt
     1621 gcctatatta aagcatcaag cattaagaaa acccgaaagt atcaattggt aagtatactt
     1681 ctgtgctgtt acgtttattc ttagctatat atagagcttt atcggtattt tttatgaatt
     1741 ccctaacgga tgattcatct gatgggattg tagtattaac acctaagctg attgttatat
     1801 ttaattcttc tactcttttt ctcatttttt cagctaaatc atatgctctt tctctttctg
     1861 tttgaggaag caatatcaca aattcttccc ctccatatct ggccactaca tcggaagagc
     1921 gctttgcaca ttccgataaa atttctgcaa ccagttttaa acaattatca ccggcctggt
     1981 gcccgaattt atcgttaaat gatttaaaat ggtctatatc tgccattatt aatgataatg
     2041 gcattgaatg ccttttacat ctgttccatt cttctctaat tttggtatcg aaggtatgtc
     2101 gattgaaaat acctgttaat ccatcatatt gggatatgat ttttagcttt ttattcgctt
     2161 cttctaattc tttattaatc cgcttcagtt catcgctgtt ttcctgcatg atttttctgt
     2221 ttttaaaatc ctgctcctgt ctcttatatc tcgtatagga aatagcaaat gacattatta
     2281 ccaatgaagt agtattcaaa tatattccaa acaacaaatc cggcgacttt tgaaaattag
     2341 gcaatagtac ggaaaaaatc aagtgtgaag taaaataaat aagaaatgaa acagagggtt
     2401 ctatatataa agcgcctgcg gttattataa ccgctgcaat atatactatt gtctgaccgt
     2461 gtgtcagttg atgcagcaat gatatcccac cgctccataa taatatgaag tttataaaga
     2521 atatttgagc attgtgaata ctgaccacat ttttagctgc atccttttcc agcttaacaa
     2581 taaccgataa aaatatagtc attgcaaaca gcaaaataaa attcattgct ccgaaatata
     2641 tctgtggctt tgtaaaaaga tttcccttac tgactataca atatacaatg agctgaataa
     2701 ttcctaatgc gataagtgtt tttgcagaga ttattaactt agctaaatta atcctacaaa
     2761 aatcaatagc aaattccttt ttgcaatcat ccgtcttaga tgttgaaaaa aggtttttaa
     2821 ttaatttgaa tgctctggct agcaatatta agccacctca tttttaggat atgcttttat
     2881 ttaattgtaa catttttaca g
//
LOCUS       DAOSOW010000242         2887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_34538_length_2887_cov_5.415254, whole genome shotgun sequence.
ACCESSION   DAOSOW010000242 DAOSOW010000000
VERSION     DAOSOW010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34538_length_2887_cov_5.415254"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLD67_06685"
     CDS             <1..250
                     /locus_tag="PLD67_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOT22241.1"
                     /translation="TQFTSAIGSAVMIETSLSFLGLGVQPPYASWGLMLSESRQFFLG
                     YPYLAVGPGIAIIITVLGFNLIGDGLNDIIINRGNSDE"
     gene            243..1193
                     /locus_tag="PLD67_06690"
     CDS             243..1193
                     /locus_tag="PLD67_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT22242.1"
                     /translation="MNETVVLNNLHIKAGSNTLVKNTSLIINEGEIFGLVGESGSGKT
                     ITAKAIINLLPKGLSYSADEIITAGKNMLTASAKEKKEHIGKNIGYIPQNTVFYLHPM
                     IKIKNQIGDGYIHHMNKTKKEALEKAEALLYKVGFKYPKIVLNYYPWQLSGGMRQRCN
                     IAMALMNDPKLIIADEPTTALDSTVQRQVMELFRKINEEMKLSILLISHDLGLVKHYS
                     HSLAVMYAGQIVESGYTNAVFSNPKHPYTQLLIKIIPSLKIKKNEPLTEIPGLISENG
                     RDIEGCLFFNRCPKAMDICKEMVAEHVDDKHEHYYRCNLE"
     gene            1202..1999
                     /locus_tag="PLD67_06695"
     CDS             1202..1999
                     /locus_tag="PLD67_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT22243.1"
                     /translation="MTKKIVISAVNIRKEYVVKKHLFTGKIKEKYVAVKGAAFDVEEG
                     KTIGIVGESGSGKSTIGEIVGGLQKPTYGCVLYFGKNIKELSEEEYHDYRKSVQFIFQ
                     DPKGSMDPNYEIIDILTMPLLTTNIAKNKDEAEKLIDKMLLRVGLDLTIKNKYPAEVS
                     GGQCQRAAIARALLVNPKLIICDEPVSALDVSIQAQILNLLRELQRELNISYIFISHD
                     IGVVNYMSDTILVMQNGNIVERGEAKEVLLHPKKEYTKILIESAFVH"
     gene            2141..2887
                     /locus_tag="PLD67_06700"
     CDS             2141..2887
                     /locus_tag="PLD67_06700"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HOT22244.1"
                     /translation="MEIKLKDNERIEDLQCKGLKIIQNKKWFCFGMDAVLLTNYCDIR
                     NNSKIVDLGTGTGIIPILLSGKRNYSKAYGIEIQPEVAEMAERSVKLNSLQEKIEILN
                     IDLKDTLKYLDAGSFDAVISNPPYKLNNSGIINPDDKKAISRHEIKCTLEDVISTASH
                     LLKQYGRFYMVHRPDRLADIICLLRKYRLEPKQIRFVHPKVKAKPNMVLIRASKNGNP
                     ELKFDPPLYIYDDEGNYTEDVHEIYGLENC"
BASE COUNT         1034 a          457 c          581 g          815 t
ORIGIN      
        1 cacccagttt acttcagcta taggctcggc agttatgatt gaaacatctc tttctttttt
       61 gggacttgga gttcagcctc catatgccag ctggggcttg atgttaagtg aatcaagaca
      121 gttttttttg ggttatccgt atttggctgt tggccccgga attgcaataa taattacagt
      181 gttaggattt aaccttattg gagacgggtt gaacgatatt attataaaca gggggaattc
      241 tgatgaatga aactgttgtg cttaacaatc tccatataaa ggcaggaagt aatacattag
      301 taaagaatac ttcactaatt attaacgaag gagaaatttt cgggcttgta ggagaatcag
      361 gctccggaaa aactattact gctaaggcta ttatcaactt gcttcctaag ggattaagct
      421 attcggctga tgaaataatt actgccggta aaaatatgtt aactgcttcg gcaaaagaaa
      481 aaaaagaaca tatagggaaa aatattggat atattcctca aaatacggta ttttatctgc
      541 atcccatgat taaaataaaa aatcaaatcg gagacggata tattcatcat atgaataaaa
      601 ccaagaagga agctttggaa aaagcagaag ctttgttata taaggttgga tttaaatatc
      661 ccaaaatagt gctgaattat tatccgtggc aattaagcgg agggatgcgt caaagatgca
      721 atattgcaat ggctttgatg aacgacccga agcttattat tgcagatgag ccgacaacgg
      781 ctttggacag cactgttcaa cgtcaggtta tggaattatt cagaaaaata aatgaagaga
      841 tgaaattgtc catacttttg atttctcatg acttaggatt ggtaaaacat tacagccaca
      901 gcttagcggt aatgtatgcc ggtcagattg tagaatcagg atacacaaat gcagtttttt
      961 ctaatcccaa acacccttac acacagcttc tgattaaaat cattccttca ttgaaaatta
     1021 agaagaatga gccattaaca gaaataccgg gtcttatatc ggaaaatggc agagatatcg
     1081 aaggatgttt gtttttcaac agatgcccca aggcaatgga tatatgtaaa gaaatggttg
     1141 ctgagcatgt agatgataag cacgagcact attacagatg taatttagaa tagaggcaat
     1201 tatgacaaaa aaaattgtga tatctgcagt aaatatcaga aaagaatatg ttgttaaaaa
     1261 gcatcttttt acaggcaaga taaaagaaaa atatgttgcc gtaaaaggtg ccgccttcga
     1321 tgttgaagaa ggaaaaacca tcgggattgt aggagaatcg ggaagcggca aatctaccat
     1381 aggcgaaata gtaggagggc ttcaaaaacc tacatatggc tgtgttcttt attttggcaa
     1441 gaatattaaa gaattatcgg aagaagaata ccatgactat agaaaaagcg tacagtttat
     1501 ttttcaagac cccaaaggct ctatggaccc taactatgaa attatcgata ttttgactat
     1561 gcctttatta acaactaata ttgcaaagaa taaggatgaa gctgaaaaac ttattgataa
     1621 aatgctttta agagtaggtc ttgatttaac cataaagaat aaatatcctg ccgaagtaag
     1681 cggagggcag tgccagaggg cagcaatagc aagagcattg cttgttaatc caaaactaat
     1741 aatatgcgat gagcctgtgt ctgccttaga tgtatccatt caggcacaga tactgaattt
     1801 actgagggag cttcaaagag agcttaatat ttcatatata ttcatatctc acgatatcgg
     1861 agtagttaat tatatgtccg acacaatatt agttatgcaa aacggaaata ttgttgaaag
     1921 aggggaggct aaggaagttc tcctgcaccc gaaaaaagaa tacacaaaga ttctcataga
     1981 aagcgcattt gtacattaac acacgtaggg tcattctgag ccgcagcatg tgtcattgct
     2041 atgaataaca ttgtcattct gaacgcagtg aagaatctca aacatgagac ccccattgtc
     2101 atttaggatg gcatataaca tactatatgg aaggtttaca atggaaatca aactgaaaga
     2161 taatgaaaga atagaagatc tgcagtgcaa aggtttaaaa atcattcaaa acaaaaaatg
     2221 gttttgtttt ggcatggatg ccgttctttt gacaaattac tgcgatataa gaaataattc
     2281 caaaattgtt gacttaggta caggtaccgg aataataccc atcctgttaa gcggtaaaag
     2341 aaattattca aaagcatacg gtattgaaat tcagcccgaa gttgcagaaa tggcggaaag
     2401 aagcgtaaaa ttaaattctc ttcaggaaaa aatagaaatt ttaaatattg atttaaaaga
     2461 tactttaaaa tacttggatg ccggttcttt tgatgcggtt atatcaaatc ctccctataa
     2521 acttaataac agcggaataa ttaatccgga tgacaaaaaa gcaatttcaa gacatgaaat
     2581 taaatgcacc ttggaggatg ttatatcgac cgcttcacat ttactgaaac aatacggcag
     2641 attctacatg gttcatcgcc ctgacaggtt ggcagatata atttgcctat taagaaagta
     2701 ccggttagaa ccgaaacaaa ttcgctttgt tcatccaaaa gttaaagcaa agccaaatat
     2761 ggttctaatt cgtgcatcta aaaacggcaa tccggaatta aaatttgacc ctcctcttta
     2821 tatttacgat gatgaaggaa attatacgga ggatgtccat gagatatatg ggttggagaa
     2881 ttgttaa
//
LOCUS       DAOSOW010000243         2886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_34552_length_2886_cov_4.201342, whole genome shotgun sequence.
ACCESSION   DAOSOW010000243 DAOSOW010000000
VERSION     DAOSOW010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34552_length_2886_cov_4.201342"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..133
                     /locus_tag="PLD67_06705"
     CDS             <1..133
                     /locus_tag="PLD67_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HOT22245.1"
                     /translation="IAFPDLGAEAIYKLRVENFPVTVIIDNKGNDLYKLGKEKYKIV"
     gene            196..1635
                     /locus_tag="PLD67_06710"
     CDS             196..1635
                     /locus_tag="PLD67_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOT22246.1"
                     /translation="MNLKVVLKVLGTVLFWESVLMVPPLIISIIDNSYDRDAFIVTIV
                     LVGFIGIMLKSIKVTVNIRKREAYASVALSWLLMSLFGALPYYVSGTAPNYIDALFET
                     ASGFTTTGATIFTNVEILPRSILFWRSFTLWIGGMGVLVFTLALAPSLGARSIFLMRA
                     EFPGPSSGKLVPKLSDTAKILYIIYAIMTGVTIILFLIGGMPIFDAFIHAFGTAGTGG
                     LSLKASGLGYYGSSFIEWTTLVLMFLFGINFSIYYGALKGNWKTVKNEELIYYFILVI
                     SAVFLIFINILPVYNYSISDSIRNSAFHVTSIITTTGFATTDINMWPLLSKMVLLILM
                     VTGACSGSTTGGLKMIRVIILFKSVIRGINHTIHPSSVSKIKIGGKTVDDEVVDSTLI
                     FFFTYILILFVSATILSLDNFDFMTTFSAALTSISNLGSGFGLIGPLGNFAGFSNLSK
                     FTMTLCMIIGRLEVLPVIALLSPTMWKKQ"
     gene            1723..2238
                     /locus_tag="PLD67_06715"
     CDS             1723..2238
                     /locus_tag="PLD67_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22247.1"
                     /translation="MNRYPYMDFLPIYPEYILAQQQEDTRQIMQTIRSEHSDVYSELQ
                     RMGLNRNLADLLITFIVNFTIRQERNLQPRQIYDRFRNQTPWFNLILRQINIRGSELD
                     RILLRIIEIILNIIRNGTRPPVPDRGWSSWESLGGILTSAPAAVSWGPNRIDVFARGP
                     RRDLIHTFEGR"
     gene            complement(2354..2638)
                     /locus_tag="PLD67_06720"
     CDS             complement(2354..2638)
                     /locus_tag="PLD67_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOT22248.1"
                     /translation="MKLILTEKHSVKIDEAYAKALDIQHTYQYITVDTANLRDAPSMD
                     GEILGTIGKGSGFYINYLEVDEEANIWCFLEFYDQNYNTVYGWCAYSNFE"
     gene            complement(2595..>2886)
                     /locus_tag="PLD67_06725"
     CDS             complement(2595..>2886)
                     /locus_tag="PLD67_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22249.1"
                     /translation="RKTLILDDIMGIWHAYENEETILIEFTSDRNINIYDNGEIEWTW
                     SAELTTRNIEGYDVSDLYFGTYPNSEFENFNSLHYINENEININGKTFRKDR"
BASE COUNT          924 a          462 c          544 g          956 t
ORIGIN      
        1 aattgcattt cccgatttag gagcagaagc aatatataaa ttgagagtgg aaaattttcc
       61 tgttaccgtg ataattgaca ataaaggaaa tgacttatat aaactaggga aagaaaagta
      121 caaaatagtt taataaagat acagttgttc aggcaaagag accggaacag aacaaaaaaa
      181 ctttccggag gaaaaatgaa tttaaaagtt gttcttaaag tacttggaac tgttttgttt
      241 tgggaatcgg tattaatggt tccgcctctt attatttcaa taatagataa cagttatgac
      301 agagatgcat ttattgttac aattgtttta gttggcttta taggaattat gctgaaaagt
      361 attaaagtta ccgtgaatat cagaaaacgt gaagcttatg cttcagtagc gctgagctgg
      421 cttttgatgt ctttatttgg agctctgcca tactacgtca gcggtactgc acccaattat
      481 atagatgctt tgtttgaaac tgcttccggg tttacgacta caggagcaac tatatttaca
      541 aatgtagaaa ttcttcctcg aagcatattg ttttggagga gttttaccct ttggattgga
      601 ggaatgggag tattggtgtt caccttggcg ttagcacctt cactgggggc acgctcaata
      661 tttttgatgc gggctgaatt tccgggaccc tcttccggaa aattagttcc aaagctttca
      721 gatacagcaa aaatattgta tattatatat gcgataatga cgggagttac aataatttta
      781 tttttgatag gcggcatgcc catatttgat gcgtttattc atgcattcgg aactgcaggt
      841 acaggcggtt tatcactgaa agcaagcggc ttaggatatt acggcagttc gtttatagaa
      901 tggacaaccc tagtgttaat gtttttattc ggaattaatt tttctattta ctatggagcg
      961 ttgaagggca actggaaaac tgtcaaaaat gaggaattga tatattattt tatattagtt
     1021 atttcagctg tattcttaat attcataaat atattacctg tttataatta ttctatatct
     1081 gattcaataa gaaactcagc cttccacgta acttcaataa ttactactac aggttttgca
     1141 acaactgaca taaacatgtg gcccttgttg tccaagatgg tcttgctgat attgatggtt
     1201 accggtgcct gctccggctc aacaacaggc ggtttaaaaa tgatacgtgt aataatactg
     1261 tttaaatcag ttataagagg aattaatcat accatacatc caagttctgt cagcaagata
     1321 aaaataggcg gtaagacagt tgatgatgaa gtagtagata gtacactgat ttttttcttt
     1381 acctatatat taattttatt tgtttcggct acgatattgt cattggataa ttttgacttt
     1441 atgacaacat tttcggctgc cttgacatcc ataagcaact taggctcagg atttggtctg
     1501 attggacctt tgggaaactt tgcaggattt tcaaacttaa gcaagtttac aatgaccttg
     1561 tgcatgataa taggaagatt ggaagtgctg ccggtaatag cattattatc tccaactatg
     1621 tggaagaagc aataattatt gttaataatt acctccgtta agggaacaaa tcttgctctg
     1681 ttaaataaaa tagagtgaga ttatttattt agggaggtta ttatgaacag atatccatat
     1741 atggattttc ttccaatata tccggaatat attttagctc agcagcaaga agatactcgt
     1801 caaattatgc aaaccatccg ctcagaacac agtgatgtat actcagaact gcagaggatg
     1861 gggttaaaca gaaatcttgc tgatttactg ataactttta ttgttaactt cacaataagg
     1921 caagagagaa atctacagcc gagacagata tatgacagat tcagaaatca aactccatgg
     1981 tttaacttaa tattaaggca gattaatatt cgaggcagtg agcttgacag aattttattg
     2041 cgcattattg agattatact aaatattatc aggaacggca caagaccgcc ggtaccggat
     2101 aggggatggt caagttggga aagcttggga ggtattttga cttctgcacc ggcagcagta
     2161 tcctggggac caaatcgtat agatgtattt gccagagggc caagaagaga tttgatacac
     2221 acttttgaag gaagataatg gactacagta attcaatgaa ttcaataacg gacatttaaa
     2281 ttaattttca gacctttgag ttaatatcaa aggtctggtt ttgacaaaaa attaaaccct
     2341 tatagggttt aatttattca aaattagaat atgcacacca tccgtacacg gtattataat
     2401 tttggtcata gaactctaaa aaacaccaga tatttgcttc ttcatccacc tcaagataat
     2461 taatataaaa cccggaacct ttacctattg ttcctaaaat ttctccatcc attgatggag
     2521 catctctaag atttgcagta tctacggtaa tatattgata tgtatgttga atatcaagag
     2581 ccttagcgta agcttcatct atctttacgg aatgtttttc cgttaatatt aatttcattc
     2641 tcgttaatat aatgcagaga attaaaattt tcaaattcgc tgtttgggta agtaccaaaa
     2701 tacaaatctg aaacatcata accttctata tttcgagttg taagttctgc agaccatgtc
     2761 cattctatct cgccgttatc ataaatattt atatttctat cgcttgtaaa ttcgattagt
     2821 atggtttctt cattttcata agcatgccat attcccatta tatcatcaag tatgagagtt
     2881 tttcta
//
LOCUS       DAOSOW010000244         2880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_34645_length_2880_cov_4.096637, whole genome shotgun sequence.
ACCESSION   DAOSOW010000244 DAOSOW010000000
VERSION     DAOSOW010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34645_length_2880_cov_4.096637"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PLD67_06730"
     CDS             complement(<1..640)
                     /locus_tag="PLD67_06730"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HOT22250.1"
                     /translation="MASGFLILISFFTRIPVGEKIEYNEENFVKALNLFTLVGVVIGF
                     LLAVVYLIFNNQISFIRGLVITVMYILISGGIHLDGACDTADGLLSGRTGDRIFEIMS
                     DSHIGAFGVIALILIILSQFVLFSFCSIETVFMMPLVGRASTIVSCWNKKYAKKIKGM
                     GTVFIENIDKNVLLINLLILFVFSMIPWYRAIMFTACFAAIAVSYFISILIEE"
     gene            complement(622..1215)
                     /gene="cobU"
                     /locus_tag="PLD67_06735"
     CDS             complement(622..1215)
                     /gene="cobU"
                     /locus_tag="PLD67_06735"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOT22251.1"
                     /translation="MSLIFVIGGARSGKSTFAELKAKEYSENVLYIATAAVTDEAMAD
                     RVKKHREQRPAFWQTLEMYSDFKTLINKAEFNQSEVVLLDCITTMIGNYMVESKLDFD
                     DCSVEDVNILENKITEEVFSLINVCKENNKKLIIVSNETGMGLVPSYYMGNYFRDISG
                     RINNKIGVQAEHMYFIFSGIPIKLKHKGEMIKWPQDF"
     gene            1440..2639
                     /locus_tag="PLD67_06740"
     CDS             1440..2639
                     /locus_tag="PLD67_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOT22252.1"
                     /translation="MRITGVVAEYNPFHSGHEYQLRMARRLSGCDCIAVVMSGNYVQR
                     GEPAIIDKFKRAEAAIYGGADIVIELPMPFSCQNAEMFAHAAIMELKKLNVDSISFGC
                     ETYNVRLLKKIALLQLTPGFNNYIKEEMKKGLSYPNAMSNALKFILNEEAEAVYSPNN
                     VLAVEYIKSAVKLNLELQYYPVKRVGMAHNDTEISGLFHSATAIRNAIMEGSFDNLKL
                     TEKSIELIKKFCKEHKKYNILNNYRDYLYYKIITCDDLEEIYEINEGLNNKIISQVYK
                     HETIDEFIMSLKSKRYTYSKLRRSLLNILLGIKKVDIINIMSTNDKYVKVLAFNNTGR
                     KAIKNAVNNGTIVINRYSDYKKNNLTPEKLMRFNITNKTSNIYYLPLENRKMNSEYFE
                     NAVYVDV"
BASE COUNT         1030 a          479 c          451 g          920 t
ORIGIN      
        1 tttcttctat cagtatggat atgaaatatg aaacagcaat tgcagcaaaa catgcagtaa
       61 acattatagc ccggtaccat ggtatcattg aaaaaacaaa taatatcaat aaattaatca
      121 ataatacatt tttatcaata ttttctataa atacagttcc catacctttt attttcttag
      181 catatttttt attccaacaa gaaactattg tgcttgcccg tcctacaaga ggcatcataa
      241 aaacagtttc aatgctgcaa aaagaaaaca gaacaaattg tgaaagtata attaaaatca
      301 atgctataac accaaaagct cctatgtggg aatcactcat tatttcaaag attctatccc
      361 ctgtccttcc ggataataag ccgtctgcag tatcacaagc accatccaaa tgtataccgc
      421 cgcttataag aatgtacata acagtaatta ccagccctct tataaaggat atttgattgt
      481 taaaaattaa ataaacaaca gccaaaagaa aacctattac cactcctact aaagtgaaaa
      541 gattcaatgc ttttacaaag ttttcttcat tatactctat tttttcccca acaggaatcc
      601 gagtaaaaaa ggaaatgaga attagaaatc ctgaggccat tttatcattt ctcctttatg
      661 tttaagttta attggtattc ctgaaaaaat aaaatacata tgctcggctt ggactccaat
      721 tttattgtta atccgcccgc ttatatccct gaaataattt cccatgtagt aagaaggtac
      781 aagacccatt cctgtttcat tagagactat tataagtttt ttattgtttt ccttgcatac
      841 attaattaat gaaaaaacct cttcggtaat tttgttttcc aatatattta catcttcaac
      901 actgcaatca tcaaaatcca atttgctttc aaccatgtaa tttccaatca tcgttgttat
      961 gcagtccaaa agaactactt cagactgatt gaattcagct ttattaataa gtgttttaaa
     1021 atcagagtac atctccaatg tctgccagaa agccggtctt tgctccctgt gctttttaac
     1081 tctatctgcc atagcctcgt ctgttacggc tgcagttgct atatacaata cgttttccga
     1141 atattctttt gcttttaatt cagcaaaggt gctcttcccg cttcttgcac cgccgataac
     1201 aaatataaga ctcattctat caccatttaa tatttttaac cattaacatt taacagtata
     1261 tcatttattt aataaaaaat aaacaatgaa ttttaaaatt ttaaggacgg gtgttctcgc
     1321 ggtgcctggc gccgggctgc attaacaaag gacggttcca aatagaaccg tcccgaaatt
     1381 ttattgacat tgatattgat tataattaag atatatgtaa agggcgaaag gataaaatta
     1441 tgagaataac aggtgttgtg gcggaatata atccatttca ttcaggacat gaatatcagc
     1501 taaggatggc aagaaggcta tcaggctgtg attgtattgc agttgtaatg agcggaaatt
     1561 atgtacagcg cggagagcct gcaattatag ataaatttaa acgggcggag gctgcaattt
     1621 atggcggtgc tgatattgtt atagagcttc ccatgccttt ttcatgtcaa aatgctgaaa
     1681 tgtttgctca tgcagcaata atggaattaa aaaagcttaa tgttgattca ataagctttg
     1741 gctgtgaaac ctacaatgta agactattga aaaaaattgc tttattacaa ttaacacccg
     1801 gttttaacaa ttatattaag gaagaaatga aaaaaggatt atcatatccc aatgccatgt
     1861 ctaatgcact taaatttata cttaatgaag aagcagaagc cgtatattca cctaataatg
     1921 tcttggctgt cgagtatata aaaagtgccg tgaaacttaa cttagaactt caatactatc
     1981 ctgtaaaacg tgtgggaatg gctcacaatg acaccgaaat ttcagggctc tttcacagtg
     2041 ctaccgcaat aagaaatgcc ataatggaag gctcttttga taatcttaag cttacggaaa
     2101 aaagtattga attgataaaa aaattctgca aggagcacaa aaaatataat atattgaaca
     2161 attataggga ttatttgtat tataaaataa ttacatgtga cgatttagaa gaaatttatg
     2221 aaataaatga aggattaaac aataaaataa tatcacaggt ttacaagcat gaaactattg
     2281 atgaatttat aatgtctctt aagtcaaagc gttataccta ttcaaagttg agaagaagcc
     2341 ttctgaatat cttactcggc atcaaaaagg ttgacataat taatattatg tcaaccaatg
     2401 ataaatatgt taaggttctt gcttttaata ataccggcag gaaggcaata aaaaatgccg
     2461 taaataatgg aaccatagta ataaacagat attccgatta taaaaagaat aatttaactc
     2521 ctgaaaaact tatgaggttt aatataacaa acaaaacatc aaacatttat tatcttccgt
     2581 tggaaaacag aaaaatgaac agcgaatatt tcgaaaatgc agtctatgta gatgtataat
     2641 tttagggggc ccgtttattt actgaagaat tgtgattgtc ataataatta tatactaagc
     2701 atataaagca ctcttggaca ataaaataaa aagggggtgt ttttatgctt agaagaaatt
     2761 ttattcctgt tttcataatc cttattattt gcttattcgt tatgaaaccg ggcatagtat
     2821 cggaaggtgt tttcaacggt cttaaaatat gggtcaacaa tatgattccg tatttgcttc
//
LOCUS       DAOSOW010000245         2855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_35064_length_2855_cov_3.407857, whole genome shotgun sequence.
ACCESSION   DAOSOW010000245 DAOSOW010000000
VERSION     DAOSOW010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35064_length_2855_cov_3.407857"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..>565)
                     /locus_tag="PLD67_06745"
     CDS             complement(99..>565)
                     /locus_tag="PLD67_06745"
                     /inference="COORDINATES: protein motif:HMM:NF040019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HOT22253.1"
                     /translation="QIALLRKNFKPIQLLQIVCTFLFGLFVTYTGKEFLLFWLPIPSL
                     YIIKLLYLFVSIVIIGIGVSFYLIPDYIPLPAEGTMNSIVELSKGKLKFSNVKVSVDS
                     SMVAVSALLSIIFLGQLKSVREGTIIAALLIGKVVGFVFKRYKPGIVEWIEN"
     assembly_gap    566..575
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<576..761)
                     /locus_tag="PLD67_06750"
     CDS             complement(<576..761)
                     /locus_tag="PLD67_06750"
                     /inference="COORDINATES: protein motif:HMM:NF040019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22254.1"
                     /translation="MKKSVKKIFLYVSGLLILAMGVNVSKAAQLGISPVSAIPYALEL
                     IWNIELGKAVFIFNIFLI"
     gene            complement(902..1264)
                     /locus_tag="PLD67_06755"
     CDS             complement(902..1264)
                     /locus_tag="PLD67_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008800843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HOT22255.1"
                     /translation="MGKFVVKEVKTGFKFDLKATNGQVIASSEIYKTKASCLKGIDSV
                     INNAPIANLEDQTVENFKVEKNPKFEIFTDKAGEYRFRLKAKNGEIIATSEGYKAMDS
                     CKNGVESVRKNAANSTIE"
     gene            1884..>2855
                     /locus_tag="PLD67_06760"
     CDS             1884..>2855
                     /locus_tag="PLD67_06760"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOT22256.1"
                     /translation="MESLKHVKLGILGMGTVASGLINIMEFNKDKVHGNINGELMVNK
                     VLVNNPNKIRNVNLPKEVYTTDAYEVINSDADIIVELIGGLSPAYEYIKAALNNKKHV
                     VTANKALIATYGEELHILAEKNNVKLMYEASVGGGIPILNTIRDNLCANEFEYIFGII
                     NGTTNYILTQMTENGLDYRQALKEAQALGFAEADPSSDVEGDDAAYKLSILSTLAFGQ
                     RIHLKDIPKEGITKISKEDIEYAKELGYNIKLLAAGIKKGNEIELNVQPAFIPAAHPL
                     STVKNEFNAVFLKGNAVGELMLYGKGAGSLPTGSAVLGDIIHIVKNAG"
BASE COUNT          977 a          480 c          484 g          904 t
ORIGIN      
        1 ccaaagtcct ccgcagtcgc actttgactt tttagatgta acattcattg ttgtattaca
       61 ttttgaacat atatacttca tttgtttcac ctcacgtgtt aattctcaat ccattcgaca
      121 attcccggtt tataacgctt aaagacaaat cctacaacct tccctattaa gagcgctgct
      181 atgatagttc cttctcttac tgatttcagc tgtcctaaaa aaattatgga cagtaaagca
      241 gaaacagcta ccatgctgct gtcaaccgaa accttaacat ttgaaaattt caacttgccc
      301 ttactaagtt cgactattga attcatagtt ccttcggcag gaagcggtat atagtccgga
      361 ataagataaa atgatacgcc tataccaata attactatac tcacaaacaa atataataat
      421 tttatgatat acaaagatgg tattggcagc caaaataata agaattcttt gccggtatat
      481 gtaacaaaca aaccgaataa aaatgtgcat acaatctgca gcaattgtat gggcttgaaa
      541 tttttcctta ataaagctat ttgcannnnn nnnnnaatta aaaaaatgtt aaatataaaa
      601 acagctttgc cgagctctat attccatata agttctaatg cataaggaat tgcgctgacc
      661 ggtgatatac caagctgtgc agccttagaa acattaactc ccattgcaag aattaatagg
      721 ccgctcacat ataaaaatat tttttttact gactttttca agtaaacttc ccccgatgta
      781 tttttttgaa cctcaatgtt attataacat ataataagtc atatatagta ttatttttat
      841 ctttaagaaa ttagaaaaaa agaacagcgg atgctgttct tcgtaacatt atggcatatt
      901 actattctat agttgaattt gctgcgtttt tacgaacgct ttctaccccg tttttacagc
      961 tgtccatggc tttataacct tccgatgtag ctataatttc tccgttcttg gcttttaggc
     1021 ggaatctgta ctctcctgcc ttgtcggtga aaatttcaaa cttaggattc ttttctactt
     1081 taaaattttc aacagtctga tcttccaaat tagcaatagg tgcattattt ataacgctgt
     1141 ctatgccttt caaacacgat gcctttgtct tgtaaatttc agagcttgca ataacttgtc
     1201 catttgtagc ctttaaatcg aatttaaaac ctgttttaac ttctttaaca acaaatttac
     1261 ccatgttccc gctcctttca ttgatagact ttttttagta tatcaatttt gtcaaaatgt
     1321 gtcaatgaac ttttttatat ctaactatga ttcctaataa ttctacatct ttttcacaat
     1381 tagtaacata taattatata tagcataata tgtattataa aagtttactg tttaaacatc
     1441 taaattttgg gtatattatt tatacaacaa tacaaagtct tacgaaaaat ctgtgagtaa
     1501 ttataaggtc gctgcaatca aattgtttgg ggagaattct ataggaatat ctcaaagtgc
     1561 agtaaaagtc tatcgaggaa tgaaaataga ggtaagattt gtaaagttga taatatagat
     1621 gattgacaaa tgaggtgatt ccaacaggat acggaaaaga aaactcaaat aaatagcacc
     1681 cggctttgaa ttgaaaagaa gttcgggtgc tatttatgtg cgttgccggg ggtaacccct
     1741 tgagtatcat tcttatggct tgcccgaaga ctttcatgaa tggaatatta tttttcttga
     1801 acttaatata aaattgtgct atattgatat agtgtttttt aaaagtacac gtgtatttat
     1861 accgggaaca agggaggatt aaaatggaat cattaaaaca tgtaaaatta ggaatattag
     1921 gcatggggac agtagcatca ggacttatta acattatgga atttaataaa gataaagtgc
     1981 atggaaatat aaacggggag ttaatggtaa ataaggttct tgttaacaac cccaataaaa
     2041 taagaaatgt taacttgccc aaagaagttt acacaaccga tgcatatgaa gtaataaaca
     2101 gcgatgcaga tataatagtt gaattaattg gaggattaag tccagcttac gaatacataa
     2161 aggcagcact caataacaaa aagcatgttg taaccgcaaa taaagctctt atcgcaactt
     2221 acggagaaga actgcatata ttggcagaaa aaaataatgt taagttaatg tatgaagcaa
     2281 gcgtgggtgg aggaattcca atactaaaca caataagaga taatctctgt gcaaatgaat
     2341 ttgaatatat ttttggaatt attaacggaa caacaaatta tattctcacg caaatgactg
     2401 aaaacggtct ggattataga caagccttaa aggaagcaca agctctcggc tttgcagaag
     2461 ctgacccttc ttccgatgtt gaaggtgatg atgcggcata caaactgtct atactgtcta
     2521 ccttagcttt tggacagaga atccacctta aagacattcc aaaggaaggt attacgaaaa
     2581 tatcaaaaga ggatattgag tatgcaaagg aattaggata caatatcaag ctgttggcag
     2641 ccggaatcaa aaaaggaaat gaaattgagc ttaatgtcca acctgcattc attcctgccg
     2701 ctcacccttt atcgaccgtt aagaatgaat ttaatgccgt atttttgaaa ggtaatgcag
     2761 tcggcgaatt aatgctgtac ggcaaaggtg caggctctct gcctacgggt agtgcagtac
     2821 ttggagatat aattcatatt gttaaaaatg caggc
//
LOCUS       DAOSOW010000246         2848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_35161_length_2848_cov_4.578947, whole genome shotgun sequence.
ACCESSION   DAOSOW010000246 DAOSOW010000000
VERSION     DAOSOW010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35161_length_2848_cov_4.578947"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /gene="dnaN"
                     /locus_tag="PLD67_06765"
     CDS             complement(<1..939)
                     /gene="dnaN"
                     /locus_tag="PLD67_06765"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOT22257.1"
                     /translation="MKLKINQNELNKAINIVQKAVSSRTPLPILSGILIEAKDNILTL
                     TATDLELGIKTYFPCEIEEEGSIVVQSRLIGDFVRKLPSNTYVFIETMGNNNMEIKCL
                     NSEINILGNSADEYPNNTFDNEGETFKIKSEVLKNLIKHTYFAAAIENIKPIFTGCLI
                     EIKNNLCTVVALDGYRMAVKKEKIDYKGDISIVIPSKTLFEILRIIEENKDETEITVS
                     ESHISFKVENTIIISNLLEGKFIDYEGIIKDNYVTVVKVNTEDMRNSIERASLLARDD
                     KNNLIIMDIKDFNMQINSASEYGNVEENISIDKEGED"
     gene            complement(1181..2515)
                     /gene="dnaA"
                     /locus_tag="PLD67_06770"
     CDS             complement(1181..2515)
                     /gene="dnaA"
                     /locus_tag="PLD67_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOT22258.1"
                     /translation="MNFEELWNEVLEIVKEDTNQVSFNTWFKPLKIVAYKNNTIYLET
                     ADDFLRNTIKKRHYNFLKNAFNYVLKKEIDIIFTIPGENIDKEESKKYNTNSLDDTDA
                     IYNGRILNPKYRFDNFIIGNSNRFAHAASLAVAEAPSTAYNPLFIYGGVGLGKTHLMH
                     AIGHYILDNNPDTYVLYVTSEKFTNDLINSIKDGTNEEFRNTYRKADVLLVDDIQFIA
                     GKESTQEEFFHTFNALHEANKQIIVSSDNPPSEIPTLEDRLRSRFEWGLIADIQPPDY
                     ETRIAILRKKAEAENYNIPDEVITYIAQHIQSNIRKLEGALIRIYAYASLTNKNEVSL
                     ELAQEALKHLISKNKKVSASDIKEVVANYFNISIDEIESKKKTKNIAYPRQIAMYIAR
                     KLTDLSLPKIGEEFGGRDHSTVLHAFNKVEEDMENSQEVKKNIEDLISMLNN"
BASE COUNT          887 a          433 c          382 g         1146 t
ORIGIN      
        1 atcttcccct tctttatcaa ttgatatatt ttcttcaaca ttgccgtatt cggaggcaga
       61 atttatctgc atattgaaat ctttaatatc cataataatc aaattatttt tatcatctct
      121 tgcaagaagc gaagctcttt caatactgtt tctcatatct tcggtattta cttttacaac
      181 agttacataa ttgtctttaa taattccttc atagtctatg aattttcctt ccaggaggtt
      241 tgaaataata attgtatttt caactttaaa ggaaatatga ctttcagaca ccgttatttc
      301 tgtttcatct ttattttctt caataatcct cagtatttca aacaatgtct tagaaggtat
      361 aactattgaa atatctccct tataatcgat tttttccttt tttacagcca tcctgtaccc
      421 gtcaagagct acaacggtac acaaattatt ttttatttca ataagacaac ctgtaaatat
      481 cggttttatg ttttctattg ctgcagcaaa atatgtatgt tttattaaat ttttaagtac
      541 ttcggatttt atcttaaaag tttctccttc attatcgaat gtattgttag gatattcgtc
      601 tgctgaattt ccaagtatat taatttcaga atttaagcat tttatttcca tattattgtt
      661 tcccatggtt tcaataaaca catatgtatt tgatggaagt tttctgacaa aatctcctat
      721 taatcttgac tgtactacaa ttgaaccttc ttcttcaatt tcacagggga aatatgtttt
      781 tattcctaat tctaaatctg ttgctgttaa agttagaata ttatcctttg cttcaataag
      841 tattcctgac aaaataggaa gaggggttct tgaagataca gccttttgta caatattgat
      901 agctttatta agttcatttt ggttaatttt taatttcata atagactcct tagtaataat
      961 atttattgtt gataaaataa ttttgcttgc catagtagaa aaatataata ataataagta
     1021 atattagtag gtattgttaa tttgttgaaa actatataaa agtttgtatt ttcaagatta
     1081 taaaaaattt atactgttga ttgttttatt atttatccaa gacatataaa atctttatgt
     1141 tttcataaat aattacaaac agtttatcaa cattttaatg ttaattatta agcattgaaa
     1201 ttaaatcttc tatatttttt ttcacttctt gactgttttc catatcttct tcaactttat
     1261 tgaaggcgtg gagaaccgtt gaatggtctc tgcctccaaa ttcttcacct attttaggca
     1321 gagataaatc agtcagtttt cttgctatat acatggcaat ttgccttgga tatgcaatat
     1381 ttttggtttt ctttttggat tctatttcat caatcgaaat attaaaataa ttagcaacaa
     1441 cttctttaat atcagatgcc gatacttttt tattttttga tatcaggtgc tttaatgctt
     1501 cctgagctaa ttcaagagat acttcgtttt tgttcgttaa ggaagcataa gcataaatac
     1561 gaattagtgc tccttcaagc ttacgaatat ttgattgtat atgctgggcg atatatgtaa
     1621 ttacttcatc aggaatatta taattttctg cttcagcctt ttttcttaat attgcaattc
     1681 tcgtttcata atccggcggc tgaatatctg caattaaacc ccattcaaaa cgtgagcgca
     1741 gcctgtcttc caaagtgggt atttcagaag gcggattatc gctcgataca ataatttgtt
     1801 tatttgcttc atgtaaagca ttaaaggtgt ggaagaattc ttcctgggta ctttcttttc
     1861 ctgcaataaa ctgaatatcg tctaccaata atacatcggc ttttctgtag gtatttctaa
     1921 attcttcatt ggtgccgtct ttaattgaat taattagatc atttgtaaac ttctcgctgg
     1981 ttacatacag tacataagtg tcgggattat tatctaatat gtaatgtcca attgcatgca
     2041 ttaaatgagt ttttccaaga cctacgcccc catatataaa caagggattg tatgctgttg
     2101 aaggtgcttc cgcaacagct aaggaagcag catgagcgaa acgattgctg tttcctatta
     2161 taaagttatc aaatctgtat tttggattta atattcttcc gttatatatt gcatccgtat
     2221 cgtctaaact atttgtgtta tatttcttgc tttcttcctt atcaatgttt tctcccggaa
     2281 tagtaaatat aatatcaatt tcttttttta ggacatagtt aaatgcattt ttaagaaaat
     2341 tataatgcct ttttttgatt gtatttctta aaaaatcgtc ggcagtttcc aaataaattg
     2401 tattgttttt atatgctact attttaagag gtttaaacca ggtgttaaaa cttacttgat
     2461 tagtatcttc ttttactatt tcaagtactt cattccataa ttcttcaaaa ttcataatta
     2521 cctccggtat tgataaaaaa aattaacaat aggttgatta atatcttata tattgataat
     2581 attatgggta taaacagttg tgtataaaaa tatgaacata aaagtaattg aatatttaat
     2641 atttgtaatg gttgataata agttgtccat tttaaatgtt gataaaaaaa gttatccaca
     2701 gacacttacc atactatcat tttttatcca caaattcaat tcaattttaa atattttaaa
     2761 aaatatttgc aatatattta aaaatatagt atattacaat agtcaacgga gacgccatct
     2821 actctctgcg ggaatgagga aatgtcct
//
LOCUS       DAOSOW010000247         2824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_35531_length_2824_cov_4.240159, whole genome shotgun sequence.
ACCESSION   DAOSOW010000247 DAOSOW010000000
VERSION     DAOSOW010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35531_length_2824_cov_4.240159"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="PLD67_06775"
     CDS             <1..773
                     /locus_tag="PLD67_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22259.1"
                     /translation="ILLYILAAKYIFDGNQLSGVVSKLSGTTKTMASGILKDPAKMEN
                     LIGMIRTFAPLTSPQTVSKVNTYLPAVEKASTLLGMYSFISKAQNFRPIQAIDAKTPA
                     DKVTALLKNGNIPVTKLMAQPLIAENMQKIVSALAMNMAKNGGLNEMLSSLTNSNNNN
                     LSDMLSSFSDGNKENTDLSNLIEKFMPMINNVMSSSNKTEEPNKPYEEEVFKKAQFDH
                     KKNDEINNKTINTPPSAEKNIRSEKHIQKPIRIRQRRR"
     gene            complement(798..1769)
                     /locus_tag="PLD67_06780"
     CDS             complement(798..1769)
                     /locus_tag="PLD67_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT22260.1"
                     /translation="MIKTNKTIAELKPGEKADGFYLIKSYEVKKTTNGKQYIDIELVD
                     KTGEINAKVWDYSKETEVLIEKNPIVKVRGEVLEWSGTRQLKVNKIREKKAEDNVQIQ
                     ELIPAAPIEPSEMLEKVKSYINKIKDNEIRTLTDNIFKKYENKLYYYPAAKRNHHSYR
                     GGLLYHNLRMLQSGEKLCQVYDFLNTDYVYAGIILHDMCKILEMESDEYGIVSDYSME
                     GKLLGHIIQGVKEIEFEGEKIGIDREKSIVLQHMVLSHHFEPEFGSPKKPMTPEAELL
                     HFLDIMDARMFDFEHFLEMVEPGQFTDKIWLLDNRNLYKLKGDNKDS"
     gene            complement(2073..>2824)
                     /locus_tag="PLD67_06785"
     CDS             complement(2073..>2824)
                     /locus_tag="PLD67_06785"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HOT22261.1"
                     /translation="DDLSYRTNDSVMMTSMEQREMKIIKTGTISAQTEDYDMFLNDLS
                     MKVSSMGGFLESNNTEVYQVYNEKKLMYGSIRIRVPQESFYELVDYLENTVDIRRKNI
                     NETDVTKEYYEKDNKVKNLEVQEQHLRELFEKATTVEEMLLIENELRRIRTEIDALNI
                     SLSDINDRAKMSTVNLEIEEVMTASLTLKTKDSVWDRSKEGFINTVNGIIRTVENIIV
                     SIVSSSPILIPAIIICIVGFLKIRKYWHHKI"
BASE COUNT          887 a          571 c          396 g          970 t
ORIGIN      
        1 aaatattatt atatatttta gcggcaaaat atatttttga cggcaatcaa ttaagcggtg
       61 ttgtttccaa gctttcggga acaactaaaa cgatggcctc ggggatactc aaggacccgg
      121 ctaagatgga aaacttaata ggcatgattc gaacctttgc tccccttact tcaccgcaaa
      181 cagtatcaaa agtaaataca tatctgccgg cagtagagaa agcaagcact ttgctcggca
      241 tgtactcatt tatcagcaag gctcagaatt ttaggccaat ccaagctata gacgccaaaa
      301 caccggcgga taaagtaaca gcattgttga aaaacggcaa tattcctgtt acaaagttaa
      361 tggcacaacc acttatagcc gaaaatatgc aaaaaatagt aagtgccttg gcgatgaata
      421 tggctaaaaa cggcggttta aacgaaatgc tttcttcttt aacaaacagc aataacaata
      481 acctaagtga tatgctttct tctttttcgg atggcaacaa ggaaaatact gatttaagca
      541 atttaataga aaaatttatg ccgatgataa acaatgtgat gtcttcttca aataaaacag
      601 aagagccaaa taaaccttat gaagaagaag tatttaagaa agctcaattt gaccacaaga
      661 aaaacgatga aataaataat aaaactatta acacgccccc ttcagcagaa aaaaacataa
      721 ggagtgaaaa acatatacaa aaaccaattc gcataagaca aagaagaaga taaaaaaaga
      781 ttcttaatcc taaggggtta agaatcttta ttgtctcctt ttaatttata aagatttctg
      841 ttatccaaaa gccatatttt atcggtaaat tgtcccggct cgaccatttc aagaaagtgt
      901 tcaaaatcaa acattcttgc atccattata tccaagaaat gcaaaagctc tgcttccggt
      961 gtcatgggtt tcttaggact gccaaactcc ggttcaaaat gatgtgataa aaccatatgc
     1021 tgcaaaacaa tacttttttc tctgtctata cctatctttt caccttcaaa ttcaatttcc
     1081 tttacccctt gaattatatg tcccagcaat ttaccttcca tgctgtaatc agatactatt
     1141 ccgtattcat cggattccat ttccaatatc ttgcacatat catgaagaat tatgcctgca
     1201 tatacataat ccgtattaag aaaatcatat acctgacaaa gcttctcgcc tgactgaagc
     1261 attcgaagat tatgatacaa aagtcctccc ctgtatgaat ggtgatttct ttttgccgca
     1321 gggtaataat acaatttatt ctcatatttc ttaaagatat tatccgttaa ggttctaatt
     1381 tcattatcct ttattttatt tatataggat tttacttttt caagcatttc gctcggctca
     1441 atcggtgcag caggtattaa ttcctgaatc tgcacattat cctcagcctt cttctctctt
     1501 attttattaa ctttaagctg tctggttcca ctccattcca agacctctcc ccttactttt
     1561 acaatggggt ttttctcaat taatacttcc gtttctttag agtaatccca cacctttgca
     1621 tttatttcac ctgtcttgtc aaccagctca atatctatat attgtttacc gttagtagtt
     1681 tttttaactt cataactctt aatcaaatag aacccgtccg ccttttctcc gggcttaagc
     1741 tcggcaattg ttttatttgt tttaatcata cttttcatct gttctcttga ttcatcacta
     1801 agcatatcag caatactttg gctcataatt ttcctcactt ttaatttgat gattaattgt
     1861 atcataggtg caagccgcaa aatggacttg cttcacaccg atacaatgaa tgcattaggg
     1921 caaagaccaa agtctttgct tcattgattt taatcattat accatactta ttcaaatcta
     1981 taaacagaat tttgtaaaac aaaaacacac ctgaataaaa ggtgtgtctt caatgcatga
     2041 gaaatgtctc aactgcacag gatgtcaaac tgttaaattt tatgatgcca atactttctt
     2101 attttaagga agccaactat acaaatgatt attgcaggaa ttagtatggg tgaacttgat
     2161 actatgctta caattatatt ttcaacagtc cttataatcc cgttaactgt attaataaaa
     2221 ccttccttcg atctgtccca cacactgtcc ttagttttta aggtaagact tgctgtcatc
     2281 acttcttcaa tttccaaatt aacggttgac attttagccc tgtcatttat atcgcttaaa
     2341 ctaatattta aagcatctat ttcagttcta attcttctaa gctcattttc tatgagaagc
     2401 atttcttcaa ccgttgttgc cttttcaaac aattctctta agtgctgctc ctgaacttcc
     2461 aagtttttta ctttgttgtc cttttcataa tattccttgg ttacatctgt ttcattgata
     2521 ttctttcttc ttatatcaac ggtattttcc agataatcga ccaattcata aaaactttcc
     2581 tgaggaactc gtattctaat gcttccatac attaattttt tctcattata tacttggtaa
     2641 acctcagtat tgttgctttc caaaaatccg cccatggaac ttactttcat tgacaaatcg
     2701 tttaaaaaca tgtcataatc ttctgtttgt gctgaaattg ttccggtctt gattattttc
     2761 atttctcttt gttccattga agtcatcatt acagaatcat ttgtccgata tgaaaggtcg
     2821 tctc
//
LOCUS       DAOSOW010000248         2818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_35656_length_2818_cov_3.260224, whole genome shotgun sequence.
ACCESSION   DAOSOW010000248 DAOSOW010000000
VERSION     DAOSOW010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35656_length_2818_cov_3.260224"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..1111
                     /locus_tag="PLD67_06790"
     CDS             59..1111
                     /locus_tag="PLD67_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOT22262.1"
                     /translation="MEIPANKILSPSELSFFCMQIAMILKSGMLISDGIDWMYEDIKE
                     GNVKDALHYLKNMLSEKVPLYVAMKNSGYFPSYIVNMSQIGSITGKLEDVMLSLSEYY
                     ERERLLKEKIRNSIFYPAMLFIMMSFIIILLVTKIFPIFEGMLEKLGASSNDSSSLSF
                     NKGIMAGRISMILVIIIIAVIAFIYILYRSEKGKKNLNMFLSNFALTKSIMKKITAYR
                     FSSAMSLLLNSGMNVDNSIDILLDIAEEPVFKENIKLCKDSMKSGDSFLDSINKLSLF
                     SSMHMQMLHMGEKIGEMDVVMNKLTDIYENEAEQAISNTVSLIEPVLVGILSVLTGVI
                     LISVMLPLMNIMSSIG"
     gene            1129..1434
                     /locus_tag="PLD67_06795"
     CDS             1129..1434
                     /locus_tag="PLD67_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22263.1"
                     /translation="MRKNFKFIFVLSIIILIAYMYIAVDSAQRKSDEESYKILSDAII
                     RSAVQCYAIEGFYPPDIEYLENNYGLLVDHNKYFISYRVFASNIIPEVQVFLKNPNQ"
     gene            1450..1932
                     /locus_tag="PLD67_06800"
     CDS             1450..1932
                     /locus_tag="PLD67_06800"
                     /inference="COORDINATES: protein motif:HMM:NF027478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4860 domain-containing protein"
                     /protein_id="HOT22264.1"
                     /translation="MDRQNQFSFQFIFIMLLYLVIVILSVIVISLGKNIYDKINEDRN
                     KNYELQVSLSYIANKIRQSDINDAVEIKDLNGIDAVVINEVYDEEEYQTWIYFYDGAI
                     YEMFTDAGSTFELSDGSKVVEADLFKIEKVKDNLYKFTAVSKEENSELYLSLYSKAVT
                     "
     gene            1932..2417
                     /locus_tag="PLD67_06805"
     CDS             1932..2417
                     /locus_tag="PLD67_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22265.1"
                     /translation="MNKVSLNSKHSLLEIILSVLIFLAAGIIMLNCFGIARFTQIRAN
                     DKVTAGAIIQSDFEIIKSLNSTDELYEFLNTYGTKESGSINTYTKYYDENWIQGSGKE
                     YAVTIVIDDENTSSGTLININISAQKNNPYPFIKAGGQQIYSIESKKFFPSIGGAYED
                     K"
     gene            2404..>2818
                     /locus_tag="PLD67_06810"
     CDS             2404..>2818
                     /locus_tag="PLD67_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22266.1"
                     /translation="MRISKTNVSVGMGGTLIITIFVVLCLMIFAVLSFVTAYSDLKLS
                     NKAQEITADYYYIHGKAEEKLAEISDVLYGIDINEEKDNILKKLSDIDGVYVDEENFK
                     LQYEVFGEENQKISVTLNLLHKDRFYYEIEAWNLSK"
BASE COUNT         1008 a          390 c          501 g          919 t
ORIGIN      
        1 tgaattttaa atatatctat aatataatta ttaaaaagta aaaagttgtg aggttattat
       61 ggaaataccg gcaaataaaa tcttatctcc ttctgaatta tcatttttct gcatgcaaat
      121 tgcaatgata ttaaaatcag gaatgcttat atccgacggc attgattgga tgtatgaaga
      181 tataaaagaa ggaaatgtca aagatgctct tcattactta aaaaacatgt tgtccgaaaa
      241 ggttcctctt tatgttgcta tgaaaaactc cggatatttc ccttcatata tagtaaatat
      301 gagtcaaata ggaagtataa ccggtaagct tgaagatgta atgctttctt tatccgaata
      361 ttatgagaga gaaagactct tgaaagaaaa aatcaggaat tctatttttt atccggctat
      421 gctttttatt atgatgtctt ttataattat attattggta acaaaaattt ttcctatatt
      481 tgaagggatg cttgaaaagc ttggtgcttc ttctaatgat tcatcttctc tttccttcaa
      541 caagggtatt atggccggaa gaatttccat gatactagta atcataataa ttgcagttat
      601 tgcttttatc tatattttat atagatccga aaagggcaaa aaaaatctta acatgttttt
      661 aagcaacttt gcattgacta aatcaataat gaaaaaaatt accgcataca ggttttcatc
      721 tgctatgtct cttttgttaa acagcggtat gaatgttgat aattcaattg atattcttct
      781 cgatattgca gaagagccgg tttttaaaga aaatattaaa ttgtgcaagg actcaatgaa
      841 gtcgggagat agttttcttg attccatcaa taaattatct ctgttttcaa gcatgcatat
      901 gcaaatgctt cacatgggtg agaaaatcgg tgagatggat gttgtaatga ataaacttac
      961 cgatatatat gaaaatgaag ccgaacaagc aatcagcaac actgtttcat tgatagagcc
     1021 cgtcctggta ggaattttgt cggttttgac aggtgttata ctgatttctg ttatgcttcc
     1081 tttaatgaac ataatgtcat ctataggata actgaaagga atgatattat gagaaaaaac
     1141 tttaaattta tttttgttct gtcaataatc attttgatag cctatatgta catagctgtt
     1201 gatagtgccc aaagaaaaag tgacgaggaa agttacaaaa ttttatctga tgcaataata
     1261 aggagtgccg ttcaatgcta tgctattgaa ggattttatc cccccgatat agaatatctt
     1321 gaaaataact acggtttgtt agtagaccat aataaatatt ttataagcta cagagttttt
     1381 gcttccaaca taattcccga agtacaggta tttctaaaaa acccgaatca ataataggaa
     1441 ggaaaaaata tggacagaca aaatcaattt tcatttcaat ttatattcat tatgcttctt
     1501 taccttgtta ttgtaatcct gtctgtaata gttatttctt tggggaaaaa tatatatgat
     1561 aagataaatg aagacagaaa caaaaattat gagctgcagg tttccctttc atacatagca
     1621 aataaaatca ggcagtctga tataaatgat gctgttgaaa ttaaagattt gaacggtatc
     1681 gatgccgttg tgataaatga agtatacgat gaagaagaat atcaaacttg gatatatttt
     1741 tatgacggtg cgatttatga aatgtttacc gatgcaggca gtacctttga attgtctgac
     1801 ggctctaagg tagtggaagc ggacctcttt aaaattgaaa aagtcaagga taatttatat
     1861 aaattcactg cagtaagcaa ggaagaaaat tcagagctgt atttatccct gtactcaaag
     1921 gcggtgactt gatgaataaa gtatccctga attcaaagca ttcacttttg gaaataattt
     1981 tatccgtttt aatattttta gccgccggaa taataatgtt aaattgcttc ggcatagcaa
     2041 ggtttacaca aataagagca aacgataagg taacagccgg tgcaataata caatcagatt
     2101 ttgaaattat aaaatcatta aatagcaccg atgaattata tgaatttctg aacacttacg
     2161 gcacaaaaga atcaggaagc atcaatacat acaccaaata ttacgatgaa aactggattc
     2221 aaggcagcgg taaggaatat gccgtaacca tagtaataga tgatgaaaat acgagctcag
     2281 gcacattgat aaatataaat atttctgccc aaaaaaataa tccctatcct tttattaaag
     2341 cagggggaca acaaatttac tctattgaaa gcaagaagtt tttcccttcc attggaggtg
     2401 cctatgagga taagtaagac taatgtttct gtaggaatgg gaggaactct tataataact
     2461 atatttgtag tgctgtgctt aatgattttt gctgtattgt catttgtaac tgcttactcg
     2521 gatttgaaac tgtccaataa agctcaggaa attacggctg actattacta tattcacggc
     2581 aaggctgaag aaaagctggc tgaaataagc gatgtattgt atggtattga cataaatgaa
     2641 gaaaaggata atattttaaa aaaattatcc gatattgacg gagtttatgt tgatgaagaa
     2701 aatttcaagc ttcaatatga agtatttgga gaggagaatc aaaaaataag cgtaacttta
     2761 aatttacttc ataaagacag attttattat gaaattgaag cttggaattt atctaaaa
//
LOCUS       DAOSOW010000249         2799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_35965_length_2799_cov_3.858965, whole genome shotgun sequence.
ACCESSION   DAOSOW010000249 DAOSOW010000000
VERSION     DAOSOW010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35965_length_2799_cov_3.858965"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            203..661
                     /locus_tag="PLD67_06815"
     CDS             203..661
                     /locus_tag="PLD67_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HOT22267.1"
                     /translation="MKTEHKEITGKGHNFEYIVSACLCGEKTRYDGKVFVSDKIKRLV
                     DEGKAITVCPEVLGGLKVPRLPCEIKNSRVINIASDDKTEHFVDGAFKTLEIAKKYGI
                     KKAILKEYSPSCGSKYIYDGTFTKTLIPGQGITTRLLQLNDIEVISDQEF"
     gene            767..2413
                     /locus_tag="PLD67_06820"
     CDS             767..2413
                     /locus_tag="PLD67_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT22268.1"
                     /translation="MQTKYLKRIIAFVIVLSMVFTTVAMAMPYGQAKKLIKNKYSWAQ
                     VQKYVKGRGIMKGYPDGMFRENDKVKRADAIIMIDRAFKLSALIDIINRDYKDVFNDV
                     DKNEYYFDAVHIARLLGITKGKGNNNFHPKQAITIREAILLIERAMDRNKYFDFDDDI
                     DLWKEYEEKELDKSATRGDIAAMLYYVLTGSKYKGVPQEYDLEEISLTIEKDEILRFD
                     DEYGKKDSIIESIEDMVDDLEYVQFTKPLNKDNTFLYYDYNSRSRSNSFVTNRNKYYI
                     DPDDEQPDLSKVSVVPKTSGVLEIEYIAYDEDGESYKGLIKITVEGKYDDLDDITFTG
                     YENTPIKFMISEFKKVFNKEFEYTPDDEISFELPDEKFGTLYFDENKDGELKDAEEIE
                     AKNSFKLKQMNFIYFNPAEDFVDEDEPVEIKYTVEDQEGNLYEGKINITVKSLLGELT
                     IDEDDDFGIEVKDYLDEFDDYDFQKIKFEKADKDDKSTLTIETNTGTRSVIGMELNIR
                     RLQDIEFEPEKDFDGTTFKYTVTTDEGEQFIGLVTVEMDD"
     gene            2558..>2799
                     /locus_tag="PLD67_06825"
     CDS             2558..>2799
                     /locus_tag="PLD67_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein, ric"
                     /protein_id="HOT22269.1"
                     /translation="MAIFNETVKKLIPTLDKFVPIVARVHGGHHPEFHDVRKIFDVIK
                     EKVKEAGAEKPNLSEEFAELREITDNYTVPGDVCES"
BASE COUNT         1116 a          342 c          550 g          791 t
ORIGIN      
        1 aaagtaaaaa attcataaag aaatatgaga atagttttat agaaaaatat tcggcggtgg
       61 gtactttatc ataactaaaa aattatttgg ataagtccga taaaatggta aaatattatt
      121 atatgtggta aaatatatta aattttatat ggagatatag ttgatgaata ctgatgaagg
      181 ggaagaagtt gatttgttga atatgaaaac cgagcataaa gaaataacag ggaagggaca
      241 taatttcgaa tatattgtaa gtgcatgcct ttgcggagaa aaaacaagat atgatggaaa
      301 agtgtttgtt tccgataaaa tcaaaagatt agttgatgaa ggaaaagcaa ttacggtatg
      361 cccggaagta ttgggcggct taaaggtgcc gaggcttcct tgtgaaataa aaaatagcag
      421 agtaattaat attgcttctg acgataagac ggagcatttt gtggatggtg ctttcaaaac
      481 gttggaaatt gctaaaaaat atggcataaa aaaagcaatt ttgaaggaat atagcccatc
      541 ctgcggcagc aagtacatat atgacggtac ttttacaaag acattgattc ccggtcaggg
      601 aataactacc agacttcttc aattaaacga cattgaggta ataagtgacc aagaatttta
      661 gctgcttaaa aatacattaa catctcatac ccctaaatat ttaacataga taacgaagaa
      721 tgtaatacaa gaaaataatg atataaatca aacatggagg tttagaatgc aaacaaaata
      781 tttaaaaaga attattgctt ttgtaattgt attgtccatg gtatttacca cggtagctat
      841 ggctatgcct tacggacagg cgaaaaagct tattaaaaac aagtacagct gggcgcaggt
      901 tcaaaaatat gtaaagggaa ggggcataat gaagggatat cccgatggta tgttccgtga
      961 aaatgataaa gttaagcgtg ccgatgccat tataatgatt gatagggcat ttaaattaag
     1021 tgctttgata gacataatca atagggatta taaagatgta tttaacgatg ttgacaaaaa
     1081 tgaatattat tttgatgcag tacacatagc tagactatta ggaataacaa agggcaaagg
     1141 aaataataat ttccacccta aacaagccat aactatcaga gaagctatat tgttaataga
     1201 acgtgcaatg gacagaaaca aatactttga ttttgatgat gacattgatt tatggaaaga
     1261 atatgaagaa aaagaattag ataaatctgc taccagaggc gatatagccg ctatgcttta
     1321 ctatgttcta acgggaagta aatataaagg tgtacctcaa gaatatgacc ttgaagaaat
     1381 atctcttaca atagaaaaag atgaaatttt aagatttgat gatgaatatg gaaaaaagga
     1441 cagtattatt gagtctattg aagatatggt agatgattta gaatatgttc aatttacaaa
     1501 gcccttaaat aaagacaaca ccttcttata ttatgattat aacagccgta gtcgttctaa
     1561 ttcatttgta accaaccgta ataaatatta tattgacccg gacgatgagc agccggatct
     1621 atcaaaagta tcagtagtgc ctaaaacaag cggtgtctta gaaatagaat acatagctta
     1681 tgatgaagat ggagaatcat ataaaggttt aatcaaaatc actgttgaag gtaaatatga
     1741 tgatttagat gatattacat ttacgggata tgagaatact cctataaaat tcatgatttc
     1801 agagtttaaa aaagtgttta ataaagaatt tgaatataca ccggatgatg aaataagctt
     1861 tgaacttccg gatgaaaaat tcgggacctt gtattttgat gaaaacaagg acggcgaact
     1921 aaaagatgcg gaagagattg aagcaaagaa ttcattcaaa ttaaaacaaa tgaattttat
     1981 ttatttcaat cctgctgaag actttgttga tgaagatgag ccggtagaaa ttaaatatac
     2041 ggtcgaagac caagaaggca atttatatga aggcaaaata aatatcacag taaaatctct
     2101 gcttggtgaa ttgactattg atgaagatga tgatttcggc atagaagtta aagactactt
     2161 ggatgaattt gatgattatg attttcaaaa aattaaattt gaaaaagcag ataaagatga
     2221 taagtcaact ctaacaatag aaacaaacac aggaacaagg tcagtaattg gaatggaact
     2281 aaatattaga cggttacaag atatagaatt tgaacctgaa aaagactttg acggaaccac
     2341 atttaaatac acggttacca cagatgaagg agaacaattt atcggattag ttacagttga
     2401 aatggacgat taaatatata atagttaaag gactgagttt gataaccaag ctcaatcctt
     2461 ttttattttt aattattgat ttaaatcaat ttattttttc aaataatttg gtagaatata
     2521 gacatcagaa aaaaagaaaa agaaagaagg aggaaatatg gcgatattta atgaaacagt
     2581 taaaaaactt attccgacat tggataaatt cgtaccgatt gtagcaagag ttcatggagg
     2641 gcatcacccg gaatttcatg atgttagaaa gatatttgac gtaatcaaag aaaaagtaaa
     2701 agaagcggga gcagaaaaac ccaacttgag tgaagaattt gctgaactgc gtgaaataac
     2761 cgataattac accgttcccg gtgatgtatg cgaaagtta
//
LOCUS       DAOSOW010000250         2799 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_35967_length_2799_cov_3.447157, whole genome shotgun sequence.
ACCESSION   DAOSOW010000250 DAOSOW010000000
VERSION     DAOSOW010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35967_length_2799_cov_3.447157"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLD67_06830"
     CDS             complement(<1..318)
                     /locus_tag="PLD67_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine / lysine / ornithine decarboxylase"
                     /protein_id="HOT22270.1"
                     /translation="MKKLNIISGIKQYRSEVDTNFHMPGHKGKPEILGELEKNLNFFD
                     ITETLGTDNLHYPTGFLKDAMNYISKVYGTKKSYMVVNGTSCGIISAIMACTNPGDKI
                     LVQR"
     gene            complement(579..848)
                     /locus_tag="PLD67_06835"
     CDS             complement(579..848)
                     /locus_tag="PLD67_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HOT22271.1"
                     /translation="MTGADLGIILAYTAGIMLVFMTSWIFFKPLKFIGKIILNSVIGA
                     IVLIIFNYFGQYTGVHIGVNEFTALIIGILGVPGFFAILLVKLLI"
     gene            complement(949..1473)
                     /locus_tag="PLD67_06840"
     CDS             complement(949..1473)
                     /locus_tag="PLD67_06840"
                     /inference="COORDINATES: protein motif:HMM:NF017856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HOT22272.1"
                     /translation="MIKIGMRNIKTTISVFICLLVFELINRNNPIFACIAAVICVQNT
                     IVDSKEIGFSRIIGTIIGGLAGAFTLFIVNTFFNHKILIFIIPLGIMLLIQICVTINM
                     KKAVVICCVVFLVITLNHDEGYILYTFNRVLDTSIGIIIALFVNKYVKIPEKLKSKIG
                     DKEELIETHENSEP"
     gene            1717..>2799
                     /locus_tag="PLD67_06845"
     CDS             1717..>2799
                     /locus_tag="PLD67_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HOT22273.1"
                     /translation="MERIVGTVVRGLRAPIITKGDDIVKITVETVLKASKTEGFSLRD
                     KDIVGITEAIVARAQGNFANIEQIALDVKQKFDSDTVGLIFPILSRNRFAVCLKGIAK
                     SFKKIYLMFSYPSDEVGNPLVDYDLLDKEGVNPWTDILKENEFRSHFKNTKHIFTGVD
                     YIKYYSDIIRESGCEVEVIFANNPISILNYTKNVLTCDIHTRNRSKRLLKSNGAETVY
                     GLDDILNQSVEGSGFNEKYGLLGTNKATEDTVKLFPRDCQSLVEKIQEKLFELTGKKI
                     EIMIYGDGAFKDPVGKIWELADPVVSPGYTEGLIGTPNEFKLKYLADNQFPHLKGDEL
                     KKAICDYIMNKDRDLKDSMESQGTTPR"
BASE COUNT          976 a          447 c          453 g          923 t
ORIGIN      
        1 tctttgcaca agtattttat ctccggggtt tgtgcatgcc ataattgccg agataatacc
       61 acatgatgta ccgttaacaa ccatgtacga ctttttagta ccgtatactt tggaaatata
      121 attcattgca tcctttaaaa agcctgtagg ataatgtaaa ttatcagttc caagtgtttc
      181 cgtaatgtca aaaaaattaa gatttttttc gagttcacct aaaatttccg gttttccttt
      241 gtgtcccggc atgtggaaat ttgtatctac ttcacttcga tactgtttaa tgcctgaaat
      301 aatattaagt tttttcatct ttttttcccg ttaataaaat atatccttct atttattggg
      361 attagtactc agctccttta agattatagt tttttatttc tttttctaga aatattattt
      421 gtggtatcaa gagtttcaac tgtaagtacc actgttattg agaaaaacgt catagcaatt
      481 gaaaatagta caatatattc aagccacata caatcaatca ccttcctgta catattatat
      541 aacagcacat taccattgtc caataaaact tgaatattct atataagcag ttttaccaac
      601 aatatagcaa aaaatccagg tacacctaat attcctataa taagtgcggt gaattcattt
      661 actcctatat gaacaccggt atactgtccg aaataattga atattattaa gactatagcg
      721 cctatcactg aatttaaaat tatttttcct ataaatttaa gcggtttaaa aaatatccag
      781 gatgtcatga atacaagcat tatccctgcc gtataagcta atattatacc taaatctgct
      841 ccagtcacaa aacaacctcc attactaaat aatccgttaa cccggtttat taattttatg
      901 aagttgtcct tatatttatg tcatctatat acattgcaga catctttact aaggctcaga
      961 attttcgtgt gtttctatca gctcttcctt atccccaatt tttgacttta gcttttcagg
     1021 aatttttaca tatttattaa caaaaagagc aattattata ccgattgagg tatccaaaac
     1081 tctgttaaag gtatatagga tataaccttc atcatgattc aaggttataa ctaaaaatac
     1141 aacgcagcaa ataactactg ctttcttcat gttaattgtt acgcagattt gaattaacaa
     1201 cataattcct aatggtataa taaaaatcaa aattttatga ttaaaaaaag tatttacgat
     1261 aaacaatgta aaggctcccg ccaggcctcc tattattgtt ccgataatcc ttgaaaatcc
     1321 tatttccttt gagtcaacaa tcgtattctg tacacagatt actgcagcta tacatgcaaa
     1381 tatgggatta tttctgttaa taagttcaaa aaccaataag catataaaga ctgaaattgt
     1441 tgtttttata ttgcgcatac ctatttttat cacgacgttt cctccgtgga atcataataa
     1501 cattttgatt tttaaacata ttgtaattaa taattataaa tttgtccagt aatatataat
     1561 aatattttac acatgtctta aaatgtatta taatgacaat gaagcaaaag ctctggcttt
     1621 tgcactgatg cattcattgt atcggtgtga agcaagccga ttttgcggct tgcacttatg
     1681 ataaatagat ttgaattcaa aattaaggag acaaaaatgg aaagaatagt gggaactgtt
     1741 gtcagaggat taagagctcc aattattaca aaaggtgatg atatagtaaa aataactgtt
     1801 gaaactgtgc tgaaggcttc caagactgaa ggcttttcat taagagataa ggatatagta
     1861 ggtattacgg aagcaattgt agcaagagct caaggtaatt ttgcaaatat tgaacaaata
     1921 gcgctggatg taaaacaaaa atttgatagt gatactgtag gtctaatatt tccgatttta
     1981 agcagaaaca gatttgctgt ttgtcttaaa ggtattgcta aatcattcaa aaaaatatat
     2041 ctgatgttca gttacccttc agatgaagtg ggtaatccct tagttgatta tgacctcctt
     2101 gataaggaag gagttaatcc ttggacagat atacttaaag agaatgaatt caggagccac
     2161 tttaagaata caaaacatat cttcacggga gtggattata ttaaatacta ttcagatatt
     2221 ataagagaaa gcggatgtga agttgaagtc atatttgcaa ataatcccat aagtattttg
     2281 aattatacaa agaatgttct tacctgtgac atccatacaa gaaacagaag caaaagattg
     2341 ttgaaatcaa atggcgctga aaccgtatac ggcttggatg atattttgaa tcaatctgta
     2401 gaaggaagcg gtttcaatga aaagtacgga ctgttaggaa caaataaggc tactgaagat
     2461 accgtaaagc tttttccgag agattgccag tccttagttg aaaaaattca agaaaaactt
     2521 tttgagctta caggcaagaa aattgaaata atgatatacg gggatggtgc ttttaaagat
     2581 cctgtcggga aaatttggga gttggcagac ccggttgtat ctccgggata tacagaagga
     2641 ttaatcggta ctcccaatga atttaaactt aaatatttgg cagacaacca atttccgcac
     2701 cttaaaggcg atgaacttaa aaaagctatt tgcgattata ttatgaataa ggaccgtgat
     2761 ttaaaagaca gcatggaatc tcaaggaact actccgaga
//
LOCUS       DAOSOW010000251         2774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_36382_length_2774_cov_3.579257, whole genome shotgun sequence.
ACCESSION   DAOSOW010000251 DAOSOW010000000
VERSION     DAOSOW010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36382_length_2774_cov_3.579257"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..253)
                     /locus_tag="PLD67_06850"
     CDS             complement(62..253)
                     /locus_tag="PLD67_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HOT22274.1"
                     /translation="MRIITAIASILIIIGALNWGLYGLSKIDIVENLFGGWKSKFGRI
                     IYLLIGAAGLYSLLYVIFA"
     gene            complement(332..1165)
                     /locus_tag="PLD67_06855"
     CDS             complement(332..1165)
                     /locus_tag="PLD67_06855"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HOT22275.1"
                     /translation="MKYKMVVLDLDGTLLNSEKTISDKNAHILIELHKRGIHIVIATG
                     RNYYMAKTLIERLKFTEPVILANNGAVVRHYVNDEFIESNYLDFSTFERIYELGLKHD
                     LNPIIHVDEYNQGYDIIYECENYEEVYLGYIKKDYKRTRCKKFKTEEINNILSVCYFN
                     EYQKLLKLKSEIEKIPGKFNIILNQNISNWSLLELLNINGCKWCALKKYIDRLGIDAN
                     QVIAIGDDNNDIELIKNSGLGIAMKNATDELKKAADSISRFSNDESGVYYELSEIFKI
                     K"
     gene            complement(1382..>2774)
                     /locus_tag="PLD67_06860"
     CDS             complement(1382..>2774)
                     /locus_tag="PLD67_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT22276.1"
                     /translation="ANPATAADYGYMFEVVDGYEDVINEVPGAKLNVTASEDGKTLTV
                     VLPVDVPYFNELVAFPTYMPVKAEVAEGNDAWATAADTYIGNGPYKVVEFSQSQLIMQ
                     KNEYYHDADRVITNELVFAFNDDDTSTLANFQNGSYLFIDQVPNDEIKSLQEQYPDEF
                     FIEGQLGTYYVSFNVNDENFKAFGEADKAKIRKALGLMIDRTYIVEEIGQAGQVPAAG
                     FVPMGLTEPDGKTEYIAKNGPKGDGAGYYSVDPDDYEKNCEEAVALLREVAESTGLYT
                     VDENGVVSKEFPTIAYLTNEGTGHEAIAINLQATFAQYGINMTIEKQEWATFLNTRKN
                     GDYSVARNGWLGDYNDPISFLDMWITSSGNNDSQFGKGEHATYAGYTYKGEGGKTWAE
                     SYDKIITEVKASKDSEERFALMHEAENILMETGAICPIYYYTDIYMKSTDLQGAFASP
                     LGYKYFMYAYVEE"
BASE COUNT          810 a          560 c          393 g         1011 t
ORIGIN      
        1 agatgcccaa aaaaacaaaa catgccgcgt gagcatgttt ttgttttttg tctgccctta
       61 ttcaagcaaa aatcacatac aataatgaat ataagccggc tgcaccaatt aatagataaa
      121 taatcctgcc aaatttactt ttccagcccc caaacaaatt ttccactata tcaatttttg
      181 atagcccgta caacccccag tttaaagctc ctataattat aagaattgaa gctattgcag
      241 taattattct catagttcct cctcaatatt gcataatatt aatacattat attttaggag
      301 ttatgaaaat atgtacaaag cttaaatatt tttatttaat tttgaatatt tctgacaatt
      361 cataatacac tccggattca tcgtttgaaa agcgcgaaat agaatctgca gccttcttta
      421 attcgtcggt tgcatttttc atagctattc ccagacctga gtttttaata agctcaatat
      481 cattattgtc gtctccgatg gcaattactt gatttgcatc tattcccagt ctgtcgatat
      541 atttttttaa tgcacaccat ttacatccat taatatttag cagttccaat agagaccagt
      601 tgcttatgtt ttgattgagt attatattga acttacctgg tattttttca atttcacttt
      661 taagttttaa taatttttga tattcattga agtagcaaac cgacaaaata ttatttattt
      721 cctctgtctt aaactttttg caccttgttc ttttataatc cttcttaata tatcctaagt
      781 atacttcttc gtaattttca cattcgtaaa taatgtcata tccttgattg tattcatcaa
      841 catgaattat cggatttaaa tcatgcttta aacctaattc atatattctc tcaaatgtag
      901 aaaaatccaa ataattgctt tcaataaatt cgtcattcac ataatgcctt acaaccgcac
      961 cattatttgc taaaataacc ggctccgtaa atttaagcct ttctattaaa gttttagcca
     1021 tataataatt tctgcctgtt gctataacaa tatgtatgcc ccttttatga agttcgataa
     1081 gtatatgtgc atttttatca cttattgttt tttcgctatt tagaagagta ccgtctaaat
     1141 ccaatactac cattttgtat ttcattttag tctcctgctc tgtactattt ttaattatac
     1201 tacagaaaac aaaaaaaata aacagaatga ttacagcata atgatactgt tcatcatccc
     1261 gagcgtagca aagatctcat aagattcttc gagctaaagc tctcagaatg acaataatgt
     1321 tattcatagg attacaaaag ccgggatttc tcccggctta gtttatattt ataatattat
     1381 tctattcttc tacataagca tacataaagt atttataacc taatgggctt gcaaaagcac
     1441 cttgaagatc tgtactcttc atgtaaatat ctgtatagta gtagattggg caaatagctc
     1501 ctgtttccat caatatattt tctgcttcat gcattaaagc aaatctttct tcagaatctt
     1561 tagaagcttt tacttcagta attatcttat catatgattc tgcccaagtt ttgccgcctt
     1621 ctcccttgta agtgtaacct gcatatgttg catgctcgcc cttgccaaat tgtgaatcgt
     1681 tgttaccgct tgatgtaatc cacatgtcaa ggaatgatat aggatcgttg tagtctccta
     1741 accagccgtt tcttgcaact gaatagtccc catttttacg agtgttaagg aaggttgccc
     1801 attcttgttt ttcaattgtc atgttaattc catattgtgc aaaagtagct tgcagattta
     1861 tggcaattgc ttcatggcct gttccttcat ttgttaaata agcaattgtc gggaattctt
     1921 ttgaaactac gccattttca tccacagtgt acaatcctgt tgattctgca acttctctta
     1981 acaaagctac agcttcttcg caattctttt cataatcatc agggtcaacg ctgtaatatc
     2041 ctgctccgtc tcctttagga ccatttttag ctatatattc tgtttttcca tccggctctg
     2101 ttaatcccat aggtacaaaa cctgctgccg gtacctgacc tgcctggccg atttcttcaa
     2161 ctatataagt tctgtcaatc ataagaccaa gagcttttct tattttcgct ttgtcagctt
     2221 caccgaaagc tttgaaattc tcatcattta cgttgaaaga aacatagtat gttccaagtt
     2281 gtccttctat aaagaattca tcaggatact gctcctgcaa tgatttaatt tcatcatttg
     2341 gaacttggtc gatgaacaag tatgagccgt tctggaagtt tgctaaagtt gaagtatcat
     2401 catcattaaa tgcaaatact aattcatttg taattacgcg gtctgcatca tgataatatt
     2461 cgttcttctg cattatcaac tggctttgtg agaattcaac aaccttgtat ggtccgttcc
     2521 caatatatgt atcagctgct gtagcccaag catcatttcc ttcggcaact tctgctttta
     2581 caggcatata tgtcgggaat gcaactaatt cattgaaata cggcacgtct acaggaagaa
     2641 ctactgtcag tgtttttccg tcttctgatg ctgttacatt aagctttgca cctggaactt
     2701 cattaatcac atcttcataa ccatcaacaa cttcgaacat gtaaccatag tctgctgcag
     2761 ttgccgggtt agct
//
LOCUS       DAOSOW010000252         2774 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_36386_length_2774_cov_3.033100, whole genome shotgun sequence.
ACCESSION   DAOSOW010000252 DAOSOW010000000
VERSION     DAOSOW010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36386_length_2774_cov_3.033100"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLD67_06865"
     CDS             <1..614
                     /locus_tag="PLD67_06865"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOT22277.1"
                     /translation="YMGASVLHEEAVFPVRQAGIPINIKNTNDPENPGTLIIRDDQAG
                     EDYANGHIITGIAGRKDFSFFYIHKEHMAHEVGIVKKALEVFEERGISIDHIPSGIDS
                     FTIVLPSESVEKISHQIVEELKKKCKTDNVQVFRNLSLITTVGIKMAYRPGISAKLFT
                     ALGNRNINIRMINQGSSEINIIVAVEDKDFEEAIRAIYDAFIK"
     gene            complement(696..1229)
                     /locus_tag="PLD67_06870"
     CDS             complement(696..1229)
                     /locus_tag="PLD67_06870"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HOT22278.1"
                     /translation="MDFIMNLEGPLLYFIIFFAKIIEVSISTVRIVFINKGEKIKGAL
                     LGFIEIMIWLIVVSSVLNNITEDPIKVFIYAIAFSLGNFFGVTIESKIAVGLSSIQVV
                     VDQKEGEVLADILREQGFGVTILEGKGKDDSVKNLLFIQLKRKKIPVATKLIKEHNPE
                     AYITVNEIKTMFGGYIK"
     gene            1640..2284
                     /gene="trmB"
                     /locus_tag="PLD67_06875"
     CDS             1640..2284
                     /gene="trmB"
                     /locus_tag="PLD67_06875"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOT22279.1"
                     /translation="MRLRYVPGQYQYLKNCEKVITEPEKQGFKLNKVFNNNNPIHVEL
                     GCGKGKFLRENAINNPHINYFGFEKSVKVAYRCAKSAFENDPPNYYIVYSNGDILKDV
                     FDAGSIDRIYLNFSDPWPKPSHYKRRLTHKNFLDVYKIVLSKNGEIHMKTDNNDLFEY
                     SLEQFEKDNWEFIMVTRDLHNSIHKEDNIMTEYEERFVNEGIKINKLIVRRKNG"
     gene            2277..2549
                     /locus_tag="PLD67_06880"
     CDS             2277..2549
                     /locus_tag="PLD67_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003729458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HOT22280.1"
                     /translation="MVNIKYIMFYLLIINLISFALFFADKQKSKRDKWRIKESTLHFE
                     SFMGGTLGSIAAMILFHHKTKKPKFVIITIIALIFNVVLVYELLTN"
     gene            complement(2625..>2774)
                     /locus_tag="PLD67_06885"
     CDS             complement(2625..>2774)
                     /locus_tag="PLD67_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOT22281.1"
                     /translation="SVVQHMIDQSPVKRVGLPEDIANAALFLASDDSSFIDGQIIKVD
                     GGFEI"
BASE COUNT         1011 a          459 c          436 g          868 t
ORIGIN      
        1 catatatggg agcatcagtt ttacatgagg aagcagtttt tccagtgaga caagcaggta
       61 ttccaataaa tattaaaaat accaacgacc ctgaaaatcc cggaacttta ataatcagag
      121 atgaccaagc aggagaagat tatgcaaacg gacatattat tacggggatt gcaggaagaa
      181 aagatttttc atttttctat attcataagg agcacatggc acatgaggtg ggtattgtaa
      241 aaaaagccct ggaagtgttt gaagaaaggg gcataagcat tgaccatatt ccttcaggca
      301 tcgacagctt tacaattgtt cttccttcag aatccgttga aaaaatcagt catcaaattg
      361 ttgaagaact taagaaaaaa tgcaaaacag ataatgtaca agtttttaga aacctcagtc
      421 ttataaccac ggtcggaatc aaaatggcgt atcgtcccgg tatatcagct aaattgttta
      481 cagcattagg aaacagaaat attaatattc gtatgataaa ccagggctca agcgaaataa
      541 acattattgt ggcagtggaa gacaaagatt ttgaagaagc tataagggca atatatgatg
      601 cattcataaa ataaattagg ggacacattg gatattcatc aataagggtg tgtccccttt
      661 accattagct gtgttttaat aattttatca aataattatt ttatatatcc gccaaacata
      721 gtttttattt catttactgt aatgtatgct tcaggattat gctcttttat taattttgta
      781 gcaacaggaa ttttctttct tttcaactga ataaataaaa gatttttaac actatcatct
      841 ttaccttttc cttctagtat cgtaacacca aatccttgtt ccctcaaaat atccgcaaga
      901 acctctccct ctttttggtc tacaacgacc tgtattgaag atagtcccac ggcaattttt
      961 gattctatag taactccaaa gaaattaccc aaagaaaatg ctatcgcgta aataaaaacc
     1021 tttataggat cttcagtaat attgtttaaa acagagctta caacaataag ccaaatcatt
     1081 atttctataa agcctaaaag ggcacccttg attttttctc ctttattaat aaagactatt
     1141 ctcactgttg aaattgaaac ttcaataatt tttgcaaaaa agattataaa gtacagcaac
     1201 ggaccctcta aattcatgat aaaatccatt tgttatgtcc acctccaaat ttattctact
     1261 cattttatca gtaatatgca atagacgcaa atattatttt attaaagaca gctgattatt
     1321 tttgttataa taatacgcag caatagggtt acataatttt atttatgtaa atctttttgt
     1381 cattctgaac ggatgtgaag aacctatact cttgtcattc gagagctagc ttgaagaatc
     1441 tcatccttgt cattctgaac gcaagtgaag aatctctact cttgtcattc tgagagcttc
     1501 cgctcgaaga atctctactc ttgtcattct gaacgcaagt gaagaatctc atcttttaaa
     1561 ccaatagtaa aatatcgtct gttaattttc attaaaataa tgtataataa attttaccaa
     1621 cataagagga aaattaaaca tgagattaag atatgttccg ggacagtatc aatatttaaa
     1681 aaattgcgaa aaagtaatta ctgagcccga aaaacaagga tttaaactga ataaagtatt
     1741 taacaacaac aatccgatac atgtggaatt aggttgcgga aagggaaaat tcttaagaga
     1801 aaatgcaatt aataaccccc atataaacta tttcggattt gagaaaagtg tcaaggttgc
     1861 atacagatgt gctaaatctg cctttgaaaa tgaccctccg aattattata tcgtttattc
     1921 aaacggagat atattaaagg atgtatttga tgcaggttca atcgacagaa tctacctgaa
     1981 cttttcagac ccttggccta aaccttctca ttacaaaaga cgattgactc acaaaaactt
     2041 cttggatgtc tataaaattg ttctttccaa gaatggtgaa attcatatga aaacagacaa
     2101 caatgaccta tttgaatact cccttgagca atttgaaaag gacaactggg aattcataat
     2161 ggtaacaaga gatttgcaca acagcatcca taaggaagat aatattatga ccgagtatga
     2221 agaacgattt gtaaatgaag gtataaaaat aaataaattg atagtgagaa ggaaaaatgg
     2281 ttaacataaa atatattatg ttctacttat taataataaa tctaatctcc tttgccttat
     2341 tctttgcaga caaacaaaaa tcaaaaagag acaaatggag aattaaagaa agcactcttc
     2401 attttgaaag ttttatgggt ggaacacttg gaagcattgc agcaatgatt ttgtttcatc
     2461 acaaaaccaa gaagcctaaa tttgttataa ttacaattat tgcattgatt tttaatgtgg
     2521 tattggtata tgaactctta acaaattaac agcagtattt caattattat atgataaaaa
     2581 taatgactga gcacatagct cagtcattta tcttactttg gtacttatat ttcaaaacca
     2641 ccgtcaactt taataatctg tccgtcaata aatgatgaat catcagaagc caagaaaagt
     2701 gcagcattag ctatatcttc aggaagtccc actcttttga caggactctg gtctatcata
     2761 tgctgaacaa caga
//
LOCUS       DAOSOW010000253         2759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_36668_length_2759_cov_2.903846, whole genome shotgun sequence.
ACCESSION   DAOSOW010000253 DAOSOW010000000
VERSION     DAOSOW010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36668_length_2759_cov_2.903846"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="PLD67_06890"
     CDS             complement(<1..1350)
                     /locus_tag="PLD67_06890"
                     /inference="COORDINATES: protein motif:HMM:NF014237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HOT22282.1"
                     /translation="MREILIGLIFALTFTPVNAEETFTNTIEYLQPDDTNDATYPEIP
                     QLPEIDKSTELIKYSPEYNKYINSAYGYEITIPNNLTLNEDIVSVKSRFESDDLVVEV
                     FYDNFYNTINSHATHRNYCNRGILKNPEFKITDEYNHSIGGLSGHVTLFERRKVKTNE
                     PDRNYYATITFEKNTYESITVFIKSAAPINIDDIMPEFKFTAKTETLKNDAVFHPEPK
                     NFDEKTRDFYDKYFINNEKTAFGIFEPTFPLYAYRLLQIEKMLDYNFPVALLYNNFSL
                     PFKTDYMNKAKELGKVVEYGLYTIDIINGREKDITLDILEGKYDDYLDNLAQSFNEYD
                     YPVLFRLNNEMNGEWVLYCSHKVGKDTDLFIDCWKYIYDKFEELGVDNLIWVWNPNEK
                     SFPDFSYNNYLCYYPGNKYVDIVGLTSYNTGNYYRGESWRSFSEAYDHFYFDYIDRFT
                     "
     gene            1519..1941
                     /locus_tag="PLD67_06895"
     CDS             1519..1941
                     /locus_tag="PLD67_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOT22283.1"
                     /translation="MVTADTFKRGFKNGISTLIDLSKVLVPVYIGVELLAASGFLNTL
                     AEIFRPVMSILGLPGEASLIIILGYFLGTYAGLGAIAAIDLNTVQITTIAIMLSISHS
                     LISETAIVKKLGASAAASITVRVFFSFFMGFLYYRIFG"
     gene            1946..2413
                     /locus_tag="PLD67_06900"
     CDS             1946..2413
                     /locus_tag="PLD67_06900"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOT22284.1"
                     /translation="MNGIMDILINLLSFLWNITKVLIPIMIVIEIFKDAKLIDKISDF
                     FYPVTKFFTLSKISGISLIFGMFFGLTLGAGAILQSVKDYNVDKRSVFLVAVFLSPCH
                     AIFEDAFIMKAAGSNILPLLAARFISAIAATYIFSRAVKNNTTDVVNISNHEK"
     gene            complement(2495..>2759)
                     /locus_tag="PLD67_06905"
     CDS             complement(2495..>2759)
                     /locus_tag="PLD67_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOT22285.1"
                     /translation="GFERLVNQLMEENFKVPDLEKVVLLYEPSDRYIDVLAKAESIRK
                     QGYIVSVYEKSKKLGKQLNQFQGYGYKKFAVYNGDETEIKELN"
BASE COUNT          809 a          467 c          463 g         1020 t
ORIGIN      
        1 agtaaatcta tctatatagt caaaataaaa atggtcatat gcttcggaga aacttctcca
       61 cgattctccc ctgtaatagt tgcctgtatt gtatgatgtc aagccgacaa tatcaacata
      121 tttatttccg ggatagtagc acaaatagtt gttgtatgaa aaatcaggga atgatttttc
      181 gttcggattc cacacccata taagattatc cacaccgagc tcttcaaatt tatcatagat
      241 atatttccag cagtctatga ataaatccgt atcctttcct actttatggg agcagtataa
      301 aacccattcg ccgttcattt cattgtttag tctgaacaac acgggataat catattcatt
      361 aaagctttga gctaagttat ctaaataatc atcgtacttt ccttccaata tgtctagggt
      421 tatatctttt tcccttccat ttattatgtc aatcgtatat aagccgtatt ccacaacctt
      481 ccctaattcc ttcgctttat tcatataatc tgttttaaaa ggcagggaaa aattgttgta
      541 cagcaatgct accggaaaat tataatcaag cattttttct atttgcaaaa gcctgtatgc
      601 atataaagga aatgtaggct caaaaatacc gaaagctgtt ttttcgttgt taatgaagta
      661 tttatcataa aagtcccttg ttttttcatc aaaattcttt ggctcgggat gaaagaccgc
      721 atcatttttc aaagtttcag tcttagccgt aaatttaaat tccggcataa tatcatcaat
      781 atttatgggt gctgctgatt ttataaatac ggttatggat tcatatgtat ttttttcaaa
      841 tgtaattgtt gcgtaataat tcctgtccgg ttcgttagtt tttacctttc tcctttcaaa
      901 gagtgttaca tgccctgaca gtccgccaat actatgattg tattcatctg taattttaaa
      961 ttcaggattt tttaatatgc ctctgttgca gtagtttcga tgtgtagcat gtgaatttat
     1021 cgtattgtaa aaattatcat aaaaaacttc cactaccagg tcatccgatt caaatcggct
     1081 ttttacgctt actatatctt cattaagcgt aagattattt ggaattgtta tttcataccc
     1141 gtaggcacta tttatatact tattgtactc tggagaatat tttattaact ctgtactttt
     1201 atcaatttcc ggaagctgtg gaatctccgg atacgttgca tcgtttgtat cgtcaggttg
     1261 aagatattct attgtgttgg taaatgtttc ttctgcattg acaggtgtaa atgtaagtgc
     1321 aaaaattagt ccgattaata tttctctcat gtaattctcc ttggtcttat tcattcttat
     1381 taattttatc aaagatagca attatagtca aactcaattg tgtcacttcc cttattaacc
     1441 gtaacgttta gaatcgcaga agttttgatt gttaataaat aatttgtatg ctataatttt
     1501 ccctgttagg aggtagcaat ggttactgca gacactttta aaagaggttt taaaaacggt
     1561 atttctactc ttatagattt atcgaaagta ttggttcccg tgtacatagg agttgagctg
     1621 cttgccgcat caggtttttt aaatacatta gccgaaatat tcaggcctgt aatgtcaata
     1681 ctgggactgc ccggtgaagc ttcactgata attatattag gttatttttt gggaacatat
     1741 gccggcttgg gtgcaattgc agctattgat cttaatacag tacaaataac aacaatagca
     1801 attatgcttt caatatccca cagtctgata tcagaaacgg caatagtaaa aaaattaggt
     1861 gcaagtgcag cagccagtat tacagtaagg gtgttttttt cattttttat gggattttta
     1921 tattatagga tatttgggtg atattatgaa tggaataatg gatatattga taaatctctt
     1981 gagcttttta tggaatataa ctaaggtact gattccaatt atgattgtaa ttgaaatatt
     2041 taaggatgca aaattaattg ataaaatatc tgactttttt tatcctgtca caaaattttt
     2101 tacattaagc aagatatccg gaatatccct tatattcggt atgtttttcg gactgacact
     2161 tggcgcgggt gccatccttc aaagtgttaa ggactacaat gtagacaaaa gaagtgtttt
     2221 tttagtagcc gtgtttttgt ccccgtgtca tgcaatattt gaagatgcat ttataatgaa
     2281 agctgcaggc tcaaatattt tgcccttgtt agcagcaaga tttatttctg caattgctgc
     2341 aacatatata ttttcacgag ctgtaaaaaa caatacgact gatgttgtaa atataagcaa
     2401 ccatgaaaaa tgagattctt cgggctgcgc cctcagaatg acgattgata ggtcattcag
     2461 tgctgcaggt gaagaatctc atagtatcta ttctttaatt taattctttt atttcggttt
     2521 cgtctccgtt gtaaacggcg aattttttgt atccgtatcc ttggaattga tttaattgct
     2581 tgcctaattt cttggatttc tcatatactg atacgatata gccttgtttt cttatacttt
     2641 cagcttttgc aagaacatct atgtatctgt ccgatggctc atataaaagc actacttttt
     2701 ccaaatcggg aactttgaag ttttcttcca tcagctggtt aaccagtctt tcaaatcct
//
LOCUS       DAOSOW010000254         2756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_36727_length_2756_cov_3.092558, whole genome shotgun sequence.
ACCESSION   DAOSOW010000254 DAOSOW010000000
VERSION     DAOSOW010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36727_length_2756_cov_3.092558"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PLD67_06910"
     CDS             complement(<1..463)
                     /locus_tag="PLD67_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOT22286.1"
                     /translation="MDTLMIAPIVGVLALLFAFYKASIVSKAEPGNDRMQEISSYIHE
                     GAMAFLAREYKSVSIFAIVMFFILGFAINWPTAIAFAAGASCSALAGYFGMTVATKAN
                     VRTTNAAMQSGMGKALDIAFSGGSVMGMTVVGLGILGTGIFWLIFEDAAVVT"
     gene            complement(593..823)
                     /gene="secG"
                     /locus_tag="PLD67_06915"
     CDS             complement(593..823)
                     /gene="secG"
                     /locus_tag="PLD67_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOT22287.1"
                     /translation="MELVVRILLIIASLILIASILLQSGKSAGMSGEIAGGAESIWGK
                     SKGRSFEGKLEKATTISAIIFVIASLVLVAIQ"
     gene            complement(996..2285)
                     /gene="eno"
                     /locus_tag="PLD67_06920"
     CDS             complement(996..2285)
                     /gene="eno"
                     /locus_tag="PLD67_06920"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOT22288.1"
                     /translation="MSIITDIYAREILDSRANPTIEVEVWTESGGYGRAGVPSGASTG
                     AFEAVELRDGDKNRYLGKGVLKAVDNVNNIIAPELIGRDSLAQVEIDKLLIELDGTPN
                     KGKLGANAILGVSIACAKAAADTLGLPLYQYLGGVNGKVLPVPMMNILNGGQHADNNV
                     DIQEFMIMPVGAESFKEALRMGAEIFHNLKIVLKGRGLNTAVGDEGGFAPNLSSNEEA
                     LVTIVEAIKKAGYIPGKDVMLALDVAASELFDAETKMYNLEGEGRKFTQTEMVDYYTE
                     LVSKYPIISIEDGMDEEDWEGWKMLTEKLGDKVQLVGDDLFVTNTVRLKKGIELNVAN
                     SILIKLNQIGTITETLDAIEMAKRAGYTAVVSHRSGETDDSTIADLVLAVNAGQIKTG
                     APSRMDRVAKYNQLLRLEDILGDTAQYKGLEVFYNIK"
     gene            complement(2297..>2756)
                     /locus_tag="PLD67_06925"
     CDS             complement(2297..>2756)
                     /locus_tag="PLD67_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOT22289.1"
                     /translation="PDVPTYDLKPEMSAFEVKNTVMEQLEKDMYKVMIINFANPDMVG
                     HTGDIEAAIKAVEAVDKCFGEIVNYIIRAEGTAIITSDHGNCEEMFDRETLDIITSHT
                     GNRVPFIVVDNKNFKLREGILADVAPTILELMGLEKPAEMTGKSLIVHNE"
BASE COUNT          763 a          640 c          401 g          952 t
ORIGIN      
        1 ccgtaactac agctgcatct tcaaaaatta accaaaatat gcctgttcct aatattccaa
       61 gaccaacaac tgtcattccc atcactgagc cgcctgaaaa tgcaatatcc aaggctttgc
      121 ccattcctga ttgcattgct gcatttgttg ttcttacatt ggcttttgtt gctactgtca
      181 taccgaaata tcccgcaagt gccgagcatg aagcccctgc cgcaaatgca attgcagtag
      241 gccaattaat tgcaaatcct aaaataaaga acattactat tgcaaaaata ctaactgact
      301 tatattctct tgcaagaaat gccattgctc cttcgtggat atatgaagat atttcttgca
      361 ttctatcatt accgggctca gccttgctta caattgatgc tttgtagaat gcaaataata
      421 aagccaaaac tcccactatg ggagctatca ttaaagtatc catttgtttc tttcctccat
      481 agtatttaca atttggcaga ttaaataaaa tctgctcatt gatatttaat aaattctaac
      541 tcaatatcaa cattagaata cttactcatt ctttccaatt aattacaaaa tattattgaa
      601 ttgcaaccaa gactaatgat gcaataacaa atataattgc tgatatagtt gttgcttttt
      661 ccagttttcc ttcaaaactt ctgcctttgc tctttcccca tatagattcg gctccacctg
      721 caatttctcc tgacattcct gccgatttgc ctgattgaag caaaatactt gcaattaaaa
      781 ttaagctagc aattattaaa agtattctta caactaattc cactaaaaca cctcctgttc
      841 ctttcttcgc ataacattag aattataaca ccaaagattt atgaaatcaa ccatattttt
      901 ttagatgagc catttctcag gcctgatttg gggacatgtc tcctaccggc agaggctatc
      961 ccataaaaag ggagcgtgtc cccatacctc taacattatt ttatattata gaaaacttcc
     1021 aagcccttat attgagcagt atcgcctaag atgtcttcca acctgagcag ttggttatat
     1081 ttagcaacac ggtccatcct tgaaggagct cctgttttaa tctgtcctgc attaactgca
     1141 agaaccaaat cagcgatagt tgaatcgtcg gtttctcccg agcggtgtga aacaaccgca
     1201 gtataacctg ctctttttgc catttcaata gcatctagtg tttctgttat tgtgcctatt
     1261 tgatttaatt ttattaatat agaattagca acatttaatt cgataccctt ttttagtctt
     1321 acagtgttgg ttacgaataa atcatcacca actaactgaa ctttatcacc cagtttttcg
     1381 gtaagcatct tccaaccttc ccagtcttct tcatccatac cgtcttctat agatattatc
     1441 ggatatttgc tcaccaattc tgtataataa tcaaccattt cagtctgagt aaactttctg
     1501 ccttcacctt ccaagttata catttttgtt tctgcatcga ataattccga agcagccaca
     1561 tcaagagcca gcataacatc tttcccggga atgtagcctg cctttttaat agcttcaact
     1621 attgtaacta atgcttcttc atttgatgat aaattcggtg caaatcctcc ttcatcacca
     1681 acggccgtat ttaatcccct tccctttaaa acgattttta aattgtggaa tatttcagct
     1741 cccattctta atgcttcttt gaaactttca gctcctacag gcataatcat gaattcctga
     1801 atatccacat tgttgtcggc atgctgcccg ccgtttaaaa tattcatcat gggtacagga
     1861 agaactttgc cgtttactcc cccaagatat tgataaaggg gtaaccccaa tgtgtctgct
     1921 gctgcttttg cgcatgcgat ggatactccc aatattgcat tcgcgcccaa ttttcctttg
     1981 ttgggagtgc cgtcaagttc aattaaaagt ttatcgattt caacttgtgc caatgaatct
     2041 cttcctatta attccggtgc aataatattg ttaacattat cgacagcttt taacacgcct
     2101 ttgcctaaat atctgttttt atcgccatct ctaagttcta cagcttcaaa tgctcctgtt
     2161 gaagctccgg atggtacgcc tgcccttccg tacccgccgc tttctgtcca tacttctact
     2221 tcaattgtag gattcgctct ggaatccaat atttctcttg cataaatatc tgttattatt
     2281 gacatactat tctcctttat tcattatgaa ctatcaaact ttttcctgtc atttctgcag
     2341 gtttttcgag tcccataagc tcaagaatcg taggggctac atctgcaaga attccttctc
     2401 ttaatttgaa atttttgttg tcaacaacta taaagggcac tctgttgccg gtgtgagaag
     2461 tgattatatc aagggtttct ctgtcaaaca tttcttcgca gtttccatgg tctgatgtaa
     2521 ttattgctgt tccttccgcc cttattatat agtttactat ttcaccgaaa catttatcca
     2581 ctgcttccac tgcctttata gctgcctcta tatctcccgt gtgtcccacc atatcaggat
     2641 ttgcaaaatt aattatcata accttataca tatccttttc taattgctcc ataacagtgt
     2701 tttttacctc aaaagcactc atttcgggct ttaagtcata agtgggcaca tcaggt
//
LOCUS       DAOSOW010000255         2751 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_36816_length_2751_cov_3.507418, whole genome shotgun sequence.
ACCESSION   DAOSOW010000255 DAOSOW010000000
VERSION     DAOSOW010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36816_length_2751_cov_3.507418"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..1228
                     /locus_tag="PLD67_06930"
     CDS             32..1228
                     /locus_tag="PLD67_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HOT22290.1"
                     /translation="MSERDKSQDKRILIGAAFLMATSAIGPGFLTQTTTFTAQLGPTF
                     GFVILVSVLFSLIAQVNIWRVICISKARGQDIANKVFPGLGYVVAILVAMGGLAFNIG
                     NVGGAGLGLNVLFGLDLNVAIAISGIIGIIIFSVKQVGNAMDKFATFCGALMIILILY
                     VSISSKPPVGQAIKETFVPSSFPYLQILTLIGGTVGGYISFSGGHRLIDAGIVGEENL
                     NRVNQSALMGMGVATAVRILLFLAVLGVVSKGVTLDASNPAADAFKVAAGTLGYKIFG
                     MVMAAAALTSVVGAAFTSVSFLRTLIKPIGDHVNITTIAFIVASTAIQIFVGSPAKVL
                     VIVGSLNGLILPLTLGTILVASKRKDIVGDYKHAQWLFILGWIVVAVTAVLGLRSLTG
                     IAALWK"
     gene            1256..2002
                     /gene="pxpB"
                     /locus_tag="PLD67_06935"
     CDS             1256..2002
                     /gene="pxpB"
                     /locus_tag="PLD67_06935"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005365486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HOT22291.1"
                     /translation="MYEKIKYLSAGDKAVVMEFGNEISKEINAKIRNVVKLIDEAKID
                     GIVELLPTYRSLMIMYDPLKIEYSDLISTLDSMSSKQVESSDEKIRIVEFPTVYGGEY
                     GPDINFVAEHNNITVDEVIKIHTGTDYLVYMMGFTPGFTYLGGMSNKIATPRLSSPRT
                     KIPAGSVGIAGAQTGMYPSETPGGWQLIGRTPLKLYDPDKEPPVMLSAGDYVRYVSVS
                     EEEYLEIKKQVEEGTYEVKVIISEGGDLRE"
     gene            1995..>2751
                     /locus_tag="PLD67_06940"
     CDS             1995..>2751
                     /locus_tag="PLD67_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HOT22292.1"
                     /translation="MSSIKVKKGGFLTTVQDLGRIGYQKFGMPVAGVMDNFSYRVANF
                     LVGNNEGEAVLEATLIGPELEFDCDMSFAVTGANMKPMLNGKEISMWESYKVTKGDIL
                     SLGVVTSGLRAYIAFSGSIEVPLLNNSKSTYLKTGIGGFKGRKLMNDDVLEIKVSDKI
                     VYGKYMDPKYIPEFKRKGNVRVVLGPQDDYFTEEGINTFLSPEGYKITKEADRMGYRL
                     DGKTIKHIDKADIISDGALFGSVQVPANGLPIIL"
BASE COUNT          925 a          414 c          600 g          812 t
ORIGIN      
        1 gactaaaata ataatatagg agattaatat tatgtcagaa agagataaat cacaggacaa
       61 aagaatcctt ataggtgctg catttttgat ggcaacatct gcaataggac ctggattttt
      121 aacacaaact acaactttta cagcacaatt aggaccgaca ttcggattcg ttattttagt
      181 atcagttctt ttttctttaa ttgctcaggt taatatttgg agagttattt gtatatcaaa
      241 agcaagaggt caagatattg caaataaagt attcccaggc ttgggatatg tagtggcaat
      301 tttggttgca atgggtggat tggctttcaa catcggtaac gttggtggag ccggattagg
      361 attaaatgtt ttattcggtt tagacttaaa tgtagctatt gcaatttcgg gaattatcgg
      421 tataatcata ttctccgtta agcaagttgg aaatgcaatg gacaaatttg caacattctg
      481 cggagcatta atgattattt tgatactata tgtttcaatt tcatcaaagc caccggttgg
      541 tcaagcaata aaagaaacat tcgttcccag ttcattccct tatctgcaaa tattgacctt
      601 gataggagga acagtaggag gatacatttc tttctcaggc ggacacagac ttattgatgc
      661 aggaatcgta ggggaagaaa atttaaacag ggttaatcaa tctgcactta tgggtatggg
      721 agttgcaact gcggttagaa tacttctatt cttagctgta ttaggtgttg tttcaaaagg
      781 agttacctta gacgcaagta accctgcagc agatgcattc aaagtagcag caggtacctt
      841 aggttataaa attttcggaa tggtaatggc tgccgctgca ttaacttctg ttgttggagc
      901 agctttcaca tcggtttctt tcttaagaac attaattaaa ccaataggcg accatgttaa
      961 tataactacc atagcattta tagtagcatc aacggcaata caaatattcg taggcagccc
     1021 tgctaaagta ttggttatag taggctcgtt aaacggactt attttaccgc ttacattagg
     1081 aacaatactt gtagcttcta agagaaaaga cattgtgggt gattacaaac atgcccagtg
     1141 gttatttata ttaggatgga tagttgttgc tgtaacagct gttttaggtt taagatcact
     1201 taccggtata gcagcattgt ggaaataaac aagaattgat gaaaggaata atgatatgta
     1261 tgaaaagata aaataccttt ctgcaggaga taaagctgta gtaatggaat tcggcaatga
     1321 aatttccaaa gaaatcaatg ctaaaatcag aaatgttgta aaactgatag atgaagctaa
     1381 aattgatgga atagttgaat tgttgcctac atacaggtct ttgatgatta tgtatgaccc
     1441 gttaaagata gagtattctg atctaatttc aactcttgat tctatgagta gcaagcaagt
     1501 ggaaagttca gatgaaaaaa ttcgaattgt ggagtttcct actgtgtatg gcggagaata
     1561 tggacctgat attaactttg ttgcagaaca taacaatatt actgtagatg aggttattaa
     1621 aattcataca ggtacggatt atctggttta tatgatggga tttactcccg gattcacata
     1681 tttaggaggt atgagcaata aaatagccac acctagactg tcctcaccaa gaacaaaaat
     1741 tcctgcaggc tctgtaggaa ttgcaggggc acaaacagga atgtatccgt cagaaacacc
     1801 cggcgggtgg cagctgattg gaagaactcc attaaagtta tatgaccctg acaaagagcc
     1861 cccggttatg cttagtgcag gcgattatgt aagatatgta tcagtaagtg aagaagaata
     1921 cttagaaatc aaaaaacaag tagaagaagg tacatatgaa gtaaaagtaa taatttccga
     1981 aggaggtgac ctgcgtgagt agcataaaag ttaagaaagg cgggttttta actactgtac
     2041 aagatttagg tcgtattggt taccaaaaat tcggaatgcc tgttgctggt gttatggaca
     2101 atttttcata tagagttgct aatttcttgg ttggaaataa tgagggcgag gctgtattgg
     2161 aagctacatt aataggaccg gaattggaat ttgactgtga tatgtcattt gctgtaacag
     2221 gtgcaaacat gaaacctatg ctaaacggca aagaaatttc aatgtgggaa agctataaag
     2281 ttacaaaggg tgacatatta tctcttggag tagttacaag cggtctcaga gcctatatag
     2341 ctttcagcgg ttcaattgaa gtacctctct taaacaacag caaatcaact tacttaaaaa
     2401 caggaatcgg cggatttaaa ggccgaaaat tgatgaatga tgatgtcttg gaaattaaag
     2461 tatccgataa aatagtgtac ggaaaatata tggatccgaa atatataccg gaattcaaaa
     2521 gaaaaggtaa tgtaagagtg gtattaggtc cccaggacga ttattttacg gaagaaggta
     2581 taaatacttt tttaagtccc gaaggatata aaataaccaa agaagcagat agaatgggat
     2641 acaggcttga cggaaaaact attaagcata tagataaagc cgacataatt tccgacggtg
     2701 ctttattcgg ctcagtgcag gtgcctgcta acggacttcc tattatactg a
//
LOCUS       DAOSOW010000256         2747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_36885_length_2747_cov_3.700594, whole genome shotgun sequence.
ACCESSION   DAOSOW010000256 DAOSOW010000000
VERSION     DAOSOW010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36885_length_2747_cov_3.700594"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..1525
                     /locus_tag="PLD67_06945"
     CDS             314..1525
                     /locus_tag="PLD67_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT22293.1"
                     /translation="MKRILSLLLSVLMVLALFTACTPASNNGNANEEPETESEAEPEA
                     EDADVIKIGVFEPLTGMNGGGGLQELGGIEYANKMYPEVLGKKIQLVVVDNKSDKGEA
                     TTAVTRLIEKDKVVAIIGSYGSAVSIAAGDIIKNAQIPAMGASCTNPMVTQGNEFYFR
                     ACFLDPFQGKVMATYAIEQGAKTAAVIMQNGDDYSVGLANFFINAFKDLTGDENSIVD
                     VSEFQLNESDFNAILTNIKAKNPDVIFAPSAATTAPLIIKQARDLGIDSLIMGGDTWE
                     NPAIIDVAGEDAEGIVLSTFFDENDPATEEARKFVEGYKKELAGKEPIIPAVAALGYD
                     AYILLRDAIERAGSTDGVAIRDALKATKDFEGVTGIINFTEEGDADKNLAVIKTVKDG
                     QFVFVDSVEVK"
     gene            1598..2491
                     /locus_tag="PLD67_06950"
     CDS             1598..2491
                     /locus_tag="PLD67_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOT22294.1"
                     /translation="MTGEIFMQQLANGISLGSLYALLAIGYTMVYGILRLINFAHGDI
                     FMMAAYFMVFSIITFNIPWFISIIITIIATVTLGVLVEKTAYRPLRNAPRMSIMISAI
                     GVSFLLENLATYLFTGVPRGFPDIKVLTTTMKLGSLSLTVATLITPFVTLMLLYVVLF
                     ITNKTKIGMAMRAVSKDFETAKLMGININQVISITFIIGSFLAAIGAVLWGSRYPSVM
                     PLMGVMPGLKCFIAAVLGGIGNTTGAVLGGFILGMAEIMLITFLPSLTGYRDAMAFVI
                     LIIVLLVKPTGLLGEKVTDKV"
     gene            2488..>2747
                     /locus_tag="PLD67_06955"
     CDS             2488..>2747
                     /locus_tag="PLD67_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005539330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22295.1"
                     /translation="MMDKLNNKNNKLILNILLIIIFASIIILLDTKKIGDAYIRRILN
                     LSAIYAIVSVSMNLVNGFTGLFSLGQAGFMAMDPLSGEVKAWV"
BASE COUNT          891 a          415 c          584 g          857 t
ORIGIN      
        1 atgattgaat tagcagatat tatggattca gcactttcta agaaagaaaa tgaaagtatg
       61 ttgaaagaac gcgtattaaa actgacttca aaattctctt tagaataaaa cagacaaccc
      121 attaatataa tagtatcagt aatgacaatc tgactgtttt ttcatatagc agcatagttt
      181 ttattttaca tacaaattat gctgtttgta tgtaaaatgt gcaagactgt ttacatagcg
      241 cggaaaattt tcttgcaata atatttttta aatgttataa tcatagaaat ttataataat
      301 gaggagagag tatatgaaaa gaattttatc tttgttatta agtgtgttga tggtgcttgc
      361 cttgtttaca gcttgtaccc cggctagcaa taacggtaat gcaaatgaag agccggaaac
      421 agagagtgag gcagagcctg aagcagagga tgcagatgtt attaaaatcg gtgtgtttga
      481 accgcttaca ggtatgaatg gcggcggtgg tcttcaggaa cttggaggca tcgaatatgc
      541 aaacaaaatg tatcctgaag tattaggcaa aaaaattcaa ttagttgtag ttgacaacaa
      601 aagtgacaaa ggcgaagcaa ctaccgcagt aacacgtctt attgaaaagg acaaggttgt
      661 tgcaataatt ggttcttacg gctcagctgt ttctattgca gccggtgata ttattaagaa
      721 tgcacagatt cctgcaatgg gtgcttcttg tacaaacccc atggtaacac agggcaatga
      781 attttacttc cgtgcatgct tccttgaccc cttccaaggt aaggttatgg ctacatatgc
      841 aattgagcag ggagcaaaga cagcagctgt tatcatgcaa aatggagatg actactcggt
      901 aggtcttgca aacttcttta ttaatgcatt taaagattta accggagacg agaacagtat
      961 agttgatgtt tcggaattcc agttgaatga atctgacttt aatgccattc ttacaaatat
     1021 caaggcaaag aatcctgacg taatatttgc tccaagtgca gcaactacgg ctccattaat
     1081 aattaaacaa gcaagagacc ttggaatcga cagcttgata atgggcggag atacatggga
     1141 aaatcctgcc attattgatg ttgccggaga agacgccgaa ggtatagttc tttcgacttt
     1201 ctttgatgaa aatgaccctg ctacggaaga agccaggaaa tttgttgaag gctacaaaaa
     1261 agaattagca ggtaaagagc ctataattcc tgcagttgca gcattaggtt atgatgcata
     1321 tatattgctc agagatgcaa tagaaagagc aggttcaacc gatggcgttg caataagaga
     1381 tgcattaaaa gcaacaaaag attttgaagg cgtaacagga attattaact tcactgaaga
     1441 gggagatgcc gataagaatc ttgctgttat taagacagtt aaggatggac aatttgtgtt
     1501 tgtagattca gtagaagtaa aataactgat atagaactat tagggcggac tgttcgttcg
     1561 cccttttaca gttttaacag atatttggag gaataatatg actggtgaaa tttttatgca
     1621 gcagcttgcc aacggcattt cattaggaag cttgtatgcc ctgttggcaa taggttatac
     1681 gatggtttat ggaattctaa gacttattaa ttttgcccat ggggatattt ttatgatggc
     1741 agcgtatttt atggtgttta gcattattac ttttaatata ccatggttta tatcaattat
     1801 aattacaata atagcaacgg ttacccttgg agtattagtt gaaaaaacag cttatagacc
     1861 attaagaaat gctcctcgta tgtccattat gatttctgcc ataggagtat catttctgtt
     1921 ggaaaactta gcaacatatt tgtttacagg tgttcccaga gggtttcccg acataaaagt
     1981 tttaactaca acaatgaaat taggaagtct ttcattaacc gttgccacat tgattacacc
     2041 ttttgttacg cttatgcttt tatatgttgt attgtttata acaaataaaa caaaaatcgg
     2101 tatggcaatg agggccgttt caaaggattt tgaaacagcc aaactaatgg gaataaatat
     2161 aaatcaagta atatccataa cttttataat aggttcattt ttagcggcga ttggagcagt
     2221 actgtggggt tcaagatatc cttccgtaat gccgctcatg ggtgttatgc ccggacttaa
     2281 gtgtttcata gcagcagtct tgggcggtat cggaaatact acaggagctg tattaggagg
     2341 atttatacta ggtatggctg aaattatgct tattacattt ctgccatccc taaccggtta
     2401 cagagatgca atggcatttg ttatattaat aattgtgttg ttggttaaac ctacagggct
     2461 tcttggagag aaggtgacgg ataaggtatg atggataaac ttaataataa aaataataaa
     2521 ttaattttga atatattgct tattattata tttgcatcga taataattct tttagataca
     2581 aaaaaaatcg gtgatgccta tataagacgt attttaaatt tatctgcaat atatgcaata
     2641 gtgagcgttt caatgaactt ggtcaacggt tttacgggat tattctcctt ggggcaggca
     2701 ggctttatgg caatggatcc tttaagtggc gaagtaaagg catgggt
//
LOCUS       DAOSOW010000257         2731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_37164_length_2731_cov_3.628924, whole genome shotgun sequence.
ACCESSION   DAOSOW010000257 DAOSOW010000000
VERSION     DAOSOW010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37164_length_2731_cov_3.628924"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1694)
                     /gene="recN"
                     /locus_tag="PLD67_06960"
     CDS             complement(36..1694)
                     /gene="recN"
                     /locus_tag="PLD67_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOT22296.1"
                     /translation="MLLSLNISNFAVFENVSVDFDSGFNVFTGETGSGKSVLINAIEL
                     ILGDRASKELIRKGKNSALIEGIFDIGDNDSLYEDLKSLNIEIDKDDFLIISRELLQN
                     GKSINKVNGRTYPLNTIKAIGSLLIDIYGQFGHESLFKKENHIKMLDSLIQNELSPLL
                     QSYNELFVKYNRVINEISTLKEKLLNKDKKIEQLQYEINEIDSAELKEFEEEELLKNI
                     KKLSNIQEIKKSLYDAIQILNSDNINNVYSIINKIKNYDEKLEEFLSRVDIQLEELKL
                     LSYDLRDYSENLDLDEKKLDNIENRMSLINRLKRKYGFSIEKINEYRNASHEELSLLL
                     KADSKLNSLITEKNEIYNLLIEKAGIISKKRKTAAEYLEKQILIELSELNMKNIEFEV
                     KINKKNISADGVDDVEFLISTNVGSDLNSVQKIVSGGEASRIMLAFKKIKSDIGQTMV
                     FDEIDMGISGKTAQMAGVKMNYIAKNNQVICVTHSPQIASISKNHFLIEKKVVDNNTF
                     STVKKLDKENKIYEIARLLSGMKITEKSINNAKELIEFNSKISE"
     gene            complement(1705..2157)
                     /locus_tag="PLD67_06965"
     CDS             complement(1705..2157)
                     /locus_tag="PLD67_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HOT22297.1"
                     /translation="MKKYTRQTKILELISKSEIETQEELAEALKNMGIDVTQATISRD
                     IKELRLVKVMSKSGKYKYATISQSQEGITDRLNKIFENSVVSIDNAMNMIIIKTIPGA
                     AQICASAIDFMGIEEIVGTLAGDDNVFVAVRKIEQVDKVLQEFKKNIR"
     gene            complement(2177..>2731)
                     /locus_tag="PLD67_06970"
     CDS             complement(2177..>2731)
                     /locus_tag="PLD67_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOT22298.1"
                     /translation="ENKTAVDIGASTGGFTDYLLKNGAKKVYAIDVGCGQLHDSLKND
                     IRVVNLENTNFRYIDTSIITDIIDIITVDVSFISLQHIIPKIVELSHNDTDIVALVKP
                     QFEAGKVNIGKKGIVKKREVHLGVLNSINNYCKTNNLKLINMDYSPIRGGAGNIEYLA
                     HLKITGNTNEINFKDLINKAFEKL"
BASE COUNT          782 a          493 c          321 g         1135 t
ORIGIN      
        1 agttttatcc ttaacttttc catttaaaac ttgttctatt cacttatttt actgttaaat
       61 tctatcaatt ccttagcatt atttatagat ttttcagtta ttttcattcc gctcaacaat
      121 ctggcaattt cataaatttt gttttctttg tctaactttt taactgtact gaatgtatta
      181 ttgtcaacaa cttttttttc aatcaaaaaa tgattttttg aaattgaagc aatttggggt
      241 gaatgcgtga cacagataac ttgattgttc tttgcaatat aattcatttt aacgccggcc
      301 atctgtgcag tcttgccgct tattcccatg tctatttcgt caaacaccat ggtttgacca
      361 atgtcgcttt ttattttttt aaaagctaac attattcttg acgcttctcc tccggaaact
      421 attttctgaa cggaatttaa gtcgcttcct acattagtgg aaattaaaaa ttcaacatcg
      481 tcaacaccgt ctgcagatat atttttttta ttgattttaa cttcaaactc aatatttttc
      541 atattaagct cgcttaattc gattaaaatc tgcttttcaa gatattctgc agcggttttt
      601 cttttcttag atataatacc tgctttttct atcaataaat tatatatttc atttttttct
      661 gtaattaaag aatttaattt tgaatcagct ttaagaagca gacttaattc ttcatgggat
      721 gcatttctgt attcattaat tttttctatt gaaaaaccat attttctttt taaacgatta
      781 ataagagaca tcctgttttc aatattatcc agtttttttt cgtcgaggtc caaattttct
      841 gaatagtccc ttaaatcata gcttaatagt tttaattcct ctaactgaat atccaccctt
      901 gaaagaaatt cttccagttt ttcgtcatag tttttaattt tatttataat gctgtaaaca
      961 ttgtttatat tatcgctgtt aagaatttgt attgcatcat acaaagattt tttaatttcc
     1021 tgtatgttag aaagcttttt gatattttta agaagctcct cttcttcaaa ttcctttaac
     1081 tctgcactat caatttcatt tatttcatat tgtaactgct caattttttt atctttattt
     1141 aatagttttt cttttaatgt gcttatttcg tttataactc ggttatattt gacaaacagc
     1201 tcattatagg actgaagcaa gggtgacaac tcattttgta taagactgtc aagcatttta
     1261 atatggtttt cttttttaaa taagctttca tgaccaaatt gaccgtaaat gtcaatcaat
     1321 aaactgccta ttgccttaat ggtgttaaga ggataagttc ttccgttaac cttgtttata
     1381 cttttgccgt tttgtaaaag ctcccttgat attattaaaa agtcatcctt atcaatttca
     1441 atgtttaaac tctttaagtc ttcatacagg ctgtcattat cgcctatatc aaaaattcct
     1501 tctattaatg cagaattttt gccttttctt attagttctt ttgaagctct atcccccaaa
     1561 ataagctcaa ttgcatttat caaaaccgat tttccggacc cggtttctcc tgtaaaaaca
     1621 ttgaatccgg agtcaaaatc aacagataca ttttcgaata ctgcaaaatt gcttatattt
     1681 aaactaagca gcatatcatc accattatct tatattcttt ttaaattcct gcaatacctt
     1741 atctacctgt tcaatttttc gcacagctac aaagacgtta tcatctcctg ccaatgttcc
     1801 tacaatttct tcaattccca taaaatcaat ggctgatgca catatctgag cagctcccgg
     1861 aattgtttta ataattatca tattcattgc attgtcgatt gaaacaacgg aattttcaaa
     1921 tatcttattc aatctgtccg ttattccttc ctggctttga cttatggtag catacttata
     1981 cttaccgctt ttagacatta cctttacaag tctaagctcc tttatatctc tggaaattgt
     2041 agcttgtgta acatcaatac ccatattttt cagggcttca gctaattcct cctgggtttc
     2101 aatttcactt tttgaaatta actcaagaat ttttgtttgt ctggtatact ttttcatttg
     2161 acacctctta gtttttttat agtttttcaa aggctttatt tattaagtct ttgaaattaa
     2221 tttcatttgt atttcctgta atttttaagt gggcaagata ttctatgttt cctgcccctc
     2281 ctcttattgg agaataatcc atattaatta atttcaaatt atttgtttta caataattat
     2341 taatactatt aagtacccct aaatgtactt ctcttttttt tactatacct tttttcccta
     2401 tgtttacctt acctgcttca aattgaggtt ttaccagtgc gacaatatct gtatcattat
     2461 gacttaattc gacaatttta ggtataatat gttgaagcga aataaaagaa acatcaactg
     2521 ttataatgtc tataatatca gtaattatag atgtatcaat atatctgaaa tttgtgtttt
     2581 ctaaattaac aactcgaata tcattcttta atgaatcatg aagctgaccg cagcccacat
     2641 ctattgcata tacttttttt gctccattct ttaacagata atccgtaaat cctccggttg
     2701 aagcaccgat atctactgct gtcttatttt c
//
LOCUS       DAOSOW010000258         2717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_37428_length_2717_cov_3.329452, whole genome shotgun sequence.
ACCESSION   DAOSOW010000258 DAOSOW010000000
VERSION     DAOSOW010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37428_length_2717_cov_3.329452"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            212..790
                     /locus_tag="PLD67_06975"
     CDS             212..790
                     /locus_tag="PLD67_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004427116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HOT22299.1"
                     /translation="MKNINTANKKELTIEQCEEIIKILKDRFEKNMNRHKDLVWDKIQ
                     VKLKANPGKLWSLNEMEKTGGEPDVIAYDENTDEYIFFDCSPESPIGRRNFCYDNEAL
                     ESRKKNKPENSAINTAHEMGIEILTEEQYRELQKLGSFDLKTSSWVKTPDKIRKLGGA
                     LFCDRRYDTVFVYHNGAESYYGARGFRGSLRV"
     gene            complement(804..1577)
                     /locus_tag="PLD67_06980"
     CDS             complement(804..1577)
                     /locus_tag="PLD67_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT22300.1"
                     /translation="MIKLNNVTSGYMGVNIIKNISLSFEKGKIISIIGKNGCGKTTLL
                     KTASKIIEPYSGDILLNGKNLFDMTSKESAKIISYLPQNRDIPNIRVYDLVMHGRFPY
                     LGFPRIPAYEDKIKVQEALHVLEIEKYKNKNIRELSGGQRQKVYIAMVLAQDTDIVFL
                     DEPTTYLDINHQLEILEIVKDLKKMGKTVVMVIHDLNSALTYSDMVCLMDKGEIKIYD
                     KPQVVYESGKIEEIFNVKCHEVGAYDKYEKQYIFSYMSA"
     gene            complement(1570..2622)
                     /locus_tag="PLD67_06985"
     CDS             complement(1570..2622)
                     /locus_tag="PLD67_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT22301.1"
                     /translation="MKAIDTLQKLSIPKSLINYKKAYNFFVISFLLILLIVLIFTSIS
                     VGSSSIEISDIFNAISGNGQLTSEYRIINYVRIPRTLSAIIAGSGLAISGLILQSVLN
                     NALASPTIIGVNSGAGLFTALATAFFPANLSIMPLAAFLGAFLATIVVYYVARKTGAS
                     RMTIILAGVAVSSLVGAMTDTVLILFPDTVISRTSFIIGGLSGVTMNRLLMPGIIIFI
                     SLITTFIFSYDLNVLSLGDQTSKSLGLNVDKTRFLFIILSSLLSGSVISFAGLLGFIG
                     LIVPHISRLIVGYDNRILIPTSVLIGSSFALFCDILARTIFAPYEVPVGIIMSFLGSP
                     FFIYLLLNKKRRLMYD"
BASE COUNT          901 a          468 c          468 g          880 t
ORIGIN      
        1 ataattaatt ttgttatgaa ttccattata atatatttat aattgaataa acaatattat
       61 tattaagttt ttaatttata actaacttta tctttgaata cagggttttg tttttttttg
      121 ctttttaata atggcaggtt ttaaatttgt gtgtttatgg gtatataaat aaaaaaccaa
      181 attaaactaa tgttatcagg aaggaatcca tatgaaaaat ataaatactg caaataaaaa
      241 agagttaacc atagaacaat gtgaggagat aattaaaata ttgaaagacc gctttgagaa
      301 aaacatgaat cgtcacaaag accttgtatg ggataaaata caagtaaagc tgaaagctaa
      361 tcccggaaag ctttggtcac taaacgaaat ggaaaaaacc ggcggagagc cggatgttat
      421 tgcttatgat gaaaatacgg atgaatacat tttttttgac tgttctccgg aaagccccat
      481 tggcagaaga aatttttgct atgacaatga agcattggag tcaagaaaga aaaacaaacc
      541 tgaaaacagc gcaattaata cagcccatga aatggggatt gagattttaa ccgaagaaca
      601 gtaccgagaa ttacagaagc ttggaagttt tgacctgaaa acatcaagct gggtcaaaac
      661 acccgataaa atcagaaaac tcggaggcgc cttattttgt gatcgccgct atgatactgt
      721 gtttgtatac cacaatggag cagaatccta ttatggtgcc aggggatttc gaggctcgct
      781 aagagtatag cactcgagtg cgattaggct gacatatatg aaaatatata ttgtttctca
      841 tatttatcat aggcacctac ctcatgacat tttacgttaa atatttcttc tatttttccg
      901 ctctcatata ccacttgagg tttatcgtaa atttttattt ctcccttatc cattaaacaa
      961 accatatcgg aataagtgag ggcgctgttt aagtcatgta taaccataac aacagtcttg
     1021 cccatttttt ttaaatcttt tacaatttcc aaaatttcca actgatgatt tatatctaag
     1081 tatgtggtcg gctcgtccaa gaaaactata tctgtatctt gtgctaatac cattgctata
     1141 tatactttct gcctttgtcc gcctgaaagt tctcttatgt ttttgttttt atatttttca
     1201 atttccaata catgaagggc ttcctgtact ttaattttat cctcatatgc aggtattctc
     1261 gggaatccta aataaggaaa tctgccgtgc ataactaaat cataaactct tatattgggt
     1321 atatctctgt tttgaggcag gtatgaaata attttggcgg attctttgct cgtcatatca
     1381 aatagatttt ttccgtttaa taaaatatct ccggaatagg gctcaataat tttcgaagct
     1441 gtttttagga gggtggtttt cccgcatccg tttttaccga taatgcttat aattttgccc
     1501 ttttcaaagg acaggcttat atttttaatt atattgacac ccatataccc tgatgttaca
     1561 ttattcaatt taatcataca taagcctcct tttcttgtta agcaataaat atataaaaaa
     1621 cggacttccc aaaaatgaca taataattcc aacaggtacc tcataaggtg caaatatagt
     1681 acgagccaat atatcgcaaa ataatgcaaa agagcttcct attaaaacac ttgtcggaat
     1741 taatattctg ttgtcatagc cgactataag ccttgatata tgtggaacta taagacctat
     1801 gaagcctaag agtcctgcaa aacttatgac actgcccgat aacagtgaag acagtattat
     1861 aaataagaaa cgggttttat ctacatttag tccaagagac ttgctagttt ggtcccctaa
     1921 ggataaaaca tttaaatcgt aactgaatat aaatgttgtt ataagggaaa taaatattat
     1981 gattccaggc attaagagcc tgttcattgt aactcccgat agtccgccga ttataaatga
     2041 agtcctgctt attacagtat ccggaaaaag aattaaaacc gtatcggtca ttgcacctac
     2101 aaggctggat accgcaacac ctgccaatat aattgtcatt cgtgatgccc ctgtttttct
     2161 tgccacgtaa tatactacaa tggtggccaa aaatgcacct aaaaatgcag ctaggggcat
     2221 tatgctaagg ttagcaggaa aaaaggctgt agccagtgct gtaaacagac ctgctcctga
     2281 atttacccca attatagtgg gacttgccaa ggcattgttt aacacagact gcaaaatcag
     2341 tccggatata gctaatccgc ttcctgctat tattgctgac agggtccttg gtatccgtac
     2401 atagttaata attctatatt cagatgttaa ctgaccatta ccggatatgg cattaaatat
     2461 atcggatatt tcgatacttg aagaaccgac ggatatgcta gtaaaaataa gtactattaa
     2521 taggattaac agaaagctta taacaaaaaa gttataagct tttttgtaat taattaaaga
     2581 tttcgggata gataatttct gcaaggtatc tatagctttc accccatttt gcatttggtt
     2641 tatagtggaa caattctttt ggcagtatga tatacctgtc attttctaca gcagataagc
     2701 tgttccaggc cggattt
//
LOCUS       DAOSOW010000259         2713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_37496_length_2713_cov_2.309631, whole genome shotgun sequence.
ACCESSION   DAOSOW010000259 DAOSOW010000000
VERSION     DAOSOW010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37496_length_2713_cov_2.309631"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..721
                     /locus_tag="PLD67_06990"
     CDS             245..721
                     /locus_tag="PLD67_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOT22302.1"
                     /translation="MTTIGLYLITGTALVVSFIKNKDKTILSLKKAWKSFENILPQFL
                     TILVIIGIALAILSPEQISRLVGSESGWIGVLVAALIGSVTLVPGFIAFPLASALLKS
                     GAGYMQIAAFISTLMMVGVVTIPLEMKYFGKKAAIIRNMEAFIFSLAVAYIMGVVM"
     gene            721..1266
                     /locus_tag="PLD67_06995"
     CDS             721..1266
                     /locus_tag="PLD67_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOT22303.1"
                     /translation="MDKIIKVIKRYRFFIILLVINTVILIVKPDIGLKSLQLTKSNAL
                     EMLSILPPVFILLGLLDVWVKRETMIKFMGEKSGIIGVLLAFLLGSAAAGPLYAAFPV
                     AAVLLKKGSKFSNVLIMIGAWSTTKIPLLLFEASSMGIKFMITRFILNIFGIAIIAYT
                     VEKLLSKREIDKIYNNAASME"
     gene            1441..2655
                     /locus_tag="PLD67_07000"
     CDS             1441..2655
                     /locus_tag="PLD67_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005946494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOT22304.1"
                     /translation="MSKYAKRMKTMEEPANIIKGLFSSMNDPGVISFGGGAIAKECLP
                     VDIVREITSDIMTVEKRGYEILSYGPVIGVKDLRDVIVKYLLAPKGVKANADNIIITT
                     GGLEAMSLMCQLFIEPGDVILVESPTFVHSVETFSMFEAKCVAVEMDDDGMVTEELEE
                     KIIKYNPKIVYVIPTFQNPTGRTLSLERRQKIAELGSKYDVIILEDDPYRDIRYSGED
                     LLPIKAFDKTGHTVLANSFSKIFSPGSRIGYVYANDDIISKMTDAKSASNSHTSLLPQ
                     IICAEFFKRGYYPEHHKLLCDIHRERRDAMLESIDKFFPEGTKRTFPDGGLFTWVELP
                     GGINTSELLKESVSNPEVKVAFVAGEGFFTEGKGKGSNCMRLSFGANPPDKIRIGIER
                     LGNLIKSKLEEK"
BASE COUNT          908 a          398 c          560 g          847 t
ORIGIN      
        1 tttaaaattt cgatagcttc atcccttttt atttctctgt cttttcttcg catttctcta
       61 aacatactta tccctccaat tagaaataaa cgttattatc tatgctgtat tatataccaa
      121 atattactaa ttgtaaattt gatagaatga tttgtattat aaattccatc agtaactttt
      181 gttacataca tacatggtga aaagtgatat aataggaaac aaaaaccaat taacggagga
      241 tgttatgacc acgattggat tgtatttaat tacaggtact gcattggttg tatcatttat
      301 taaaaataag gataaaacaa tactttcatt gaagaaggcg tggaaatcat tcgaaaatat
      361 tctacctcaa tttttgacta tacttgtcat tataggaatt gcacttgcta tactctcacc
      421 tgaacaaatt tcaaggttgg ttggaagcga atcggggtgg attggagtat tggttgctgc
      481 tctcatcggc tcagtaactc ttgtgcccgg ttttatagca tttcctcttg cttccgcatt
      541 attaaagagc ggggcaggct acatgcagat agcggcattt atttcaacac ttatgatggt
      601 tggagtagta accatccctc ttgaaatgaa atatttcggc aaaaaagctg caataataag
      661 gaatatggag gcatttattt tttccttagc agtagcatac attatggggg ttgtaatgtg
      721 atggataaaa ttataaaagt aattaaaaga tatagatttt ttataatcct cttagttatc
      781 aatactgtaa ttttaattgt aaaaccggat attggactaa aatctttaca gttaacaaaa
      841 agcaatgccc ttgaaatgtt gtccatactg ccgcctgttt ttattctgtt ggggcttttg
      901 gatgtttggg taaaaagaga aacaatgatt aaattcatgg gtgaaaaatc agggattatc
      961 ggtgtcttgc tagcattttt attaggttct gccgcagcag gtccattata tgcggctttt
     1021 ccggtggctg cagtgcttct taaaaaggga agcaaatttt caaacgtttt aattatgatt
     1081 ggtgcctggt ctacaacaaa aataccatta ttattattcg aagcatcatc aatgggaata
     1141 aaatttatga ttacaagatt catattgaat atatttggga ttgccattat agcatataca
     1201 gttgaaaagt tattatcaaa gagagaaata gataaaatct ataataatgc tgcatcaatg
     1261 gaataattga tgacatacat agagtttgat aacagtctaa gcaccggcat gccggtgctt
     1321 ttacatatat ttgaactttt atttggctaa agtaattttt gatggtataa tttaagaaat
     1381 ctaataatgc tcacggaaga ttactggtaa ggataataat aagaattaag gagaagtaat
     1441 atgtcaaaat atgcaaaacg aatgaaaaca atggaggagc cggccaatat tataaaaggt
     1501 ttattcagct ccatgaatga tcccggtgtg atttcctttg gcggcggagc aattgcaaag
     1561 gaatgtcttc ctgttgatat tgtaagagaa attacctctg atattatgac agttgagaaa
     1621 agaggttatg aaatactgtc ctacggacct gtaattggtg ttaaggattt aagagatgta
     1681 atagtaaagt atctactggc tccaaaaggg gtcaaggcaa atgccgacaa tattataatt
     1741 acaaccggcg gattggaagc aatgagctta atgtgtcagc ttttcattga gccgggtgat
     1801 gtaattttgg tggaatctcc taccttcgtt cattctgttg aaacattcag catgtttgaa
     1861 gcaaaatgtg ttgctgttga aatggatgat gatgggatgg ttaccgagga gctggaggaa
     1921 aaaattatta aatacaatcc aaaaatagta tatgtaattc ctacattcca aaatcctaca
     1981 ggcaggacct tatccttgga gagaagacag aaaattgcag agcttggaag caagtatgat
     2041 gtaattatat tagaagatga cccataccgg gatataaggt acagcggcga agatttattg
     2101 ccgattaaag catttgataa aactggtcac actgtgctgg ctaatagttt ttcgaagata
     2161 ttttcgccgg gaagcagaat aggatatgtg tatgcaaatg atgatataat atcaaagatg
     2221 acagatgcaa agtcagcttc aaattcacat acctcgctgt tgcctcagat aatttgtgcc
     2281 gagtttttca agagaggata ctaccccgaa catcacaagc tgctgtgcga tattcacaga
     2341 gaaagaagag atgcaatgct ggaaagtata gataagtttt tccctgaagg aactaaaagg
     2401 acatttcctg acggaggatt attcacatgg gtggaattgc ccggtggaat aaatacttca
     2461 gaattattaa aagaatctgt ttcaaatcct gaagttaagg ttgcctttgt tgccggagag
     2521 ggatttttta ccgaaggtaa gggaaagggc agtaattgca tgagacttag ctttggtgcc
     2581 aatcctcccg ataaaataag aattggaatt gaaagattgg gaaatttaat taaatctaaa
     2641 ttggaggaaa aatgataaag aataagctca aagaaaagtt aaatgccgga aaggcggcaa
     2701 ttggaacgtt cat
//
LOCUS       DAOSOW010000260         2712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_37511_length_2712_cov_3.643583, whole genome shotgun sequence.
ACCESSION   DAOSOW010000260 DAOSOW010000000
VERSION     DAOSOW010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37511_length_2712_cov_3.643583"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..635)
                     /locus_tag="PLD67_07005"
     CDS             complement(48..635)
                     /locus_tag="PLD67_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22305.1"
                     /translation="MKNRHIYIILTRTNTVLSNLISIIKNDEYTHASISLNKNLNTMY
                     SFGRKYTYNPFIGCFVKENLYEGVFGFHNKLKGQVLELNATEEQYDKVLKLLDEFISN
                     SKYLKYNYLGLINSLFNRESICEDRFLCSEFVYYILNKAGIAELNKPRNLVRPQDLLN
                     LKSRVIFKGNLKNKKFIKKYENNFIEKYSAVGTLS"
     gene            complement(759..1871)
                     /gene="cobT"
                     /locus_tag="PLD67_07010"
     CDS             complement(759..1871)
                     /gene="cobT"
                     /locus_tag="PLD67_07010"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HOT22306.1"
                     /translation="MHVEFYKNFNIKEASEKELESVLNEIIKGIKKPDSISAEGMQKR
                     LDAKIKPLRSLGVLEDIAVQLAGIQGTEFPEVKGKAVLLMAGDHGVVKEGVSAAPQEV
                     TAQLYYSYLNGGGGINVLTGHAGAKLICNDIGICGRLEPPDLMANRIKNGTDNIAEGP
                     AMTREEALRSLLTGAKIASEAIESGINILAIGEVGIGNTTPSAALITVLTGCSVEEAT
                     GSGTGLKGDALKHKCNIIKKAIEINKPNLNDPIDILSKIGGLEIAAMVGAILEAAYQN
                     VPTILDGIISSAAALIASKLNPIVVEYMIPSHSSEEKGELVALKHMGLEPRLFLNMRL
                     GEGTGAALMFHIVEAAVKIADEMATFETSGVTTGDF"
     regulatory      complement(2003..2177)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2263..>2712)
                     /locus_tag="PLD67_07015"
     CDS             complement(2263..>2712)
                     /locus_tag="PLD67_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014118146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HOT22307.1"
                     /translation="LAELLSKNNTVIDPAPYSGDEFNEVAKKALLTDVFFTSVNGVAE
                     TGELVNIDGTGNRVAGSLFGHKKIYFVFGTNKIEPNLEKAIWRARNIAAPQNSKRLGY
                     NTPCAVKADRCYDCSSSERICNTLNIHLKKMKYVEAECIIINENLGM"
BASE COUNT          775 a          568 c          386 g          983 t
ORIGIN      
        1 ataatagtat tttaccactt tatcggactt atccaaataa tttttagtta tgataaagta
       61 cccaccgccg aatatttttc tataaaatta ttctcatatt tctttatgaa ttttttattt
      121 tttaaatttc ctttaaagat aaccctgctc ttcagattta ataaatcctg tggccttact
      181 aaatttctgg gtttgttaag ttctgcaata ccggctttgt ttaaaatata atatacaaat
      241 tcagagcata aaaatctgtc ttcgcaaatg gattctctat taaacaaact gtttatcagt
      301 cctaaataat tgtattttag atatttgctg tttgaaataa actcatccaa tagttttaaa
      361 accttatcat attgctcttc ggtggcatta agttccaata cttgtccctt aagtttattg
      421 tgaaatccga aaactccttc atataaattt tctttcacaa aacacccgat aaatggatta
      481 taggtatatt ttctcccaaa actatacatg gtgtttaaat tcttattcaa ggatatagaa
      541 gcatgtgtgt actcatcatt tttaattatg ctgataagat ttgataatac cgtattggtt
      601 cttgttagaa taatataaat atgtctgttt ttcataatcc ctcctacgta cggattgatt
      661 atggcaaaac agacttaaat atatataaaa aatatattaa tgattcatta aaatcatatt
      721 taatttactt aatgaaatta tcgctaactt ccacaagatt aaaagtctcc ggtcgttact
      781 cctgaagttt caaaggtagc catttcatca gcgattttta cggctgcttc aactatgtga
      841 aacataagcg cagcacctgt accttcccct aatctcatat ttaaaaataa tcttggctct
      901 aatcccatgt gtttcaaagc taccagttca cctttttcct ccgatgaatg agatggtatc
      961 atatattcaa caacaatcgg attgagtttt gatgctataa gtgctgcagc ggatgatatt
     1021 attccatcca atatcgttgg aacgttttga taagcagctt ctaaaattgc accaaccatt
     1081 gcagcaattt ctaacccgcc tatttttgat aatatatcta tcgggtcatt aagattaggt
     1141 ttgtttattt caattgcctt ttttattata ttacatttat gctttaatgc atcacccttt
     1201 aatccggtac cgctgcctgt agcttcttca acgctgcatc ctgttaatac cgtgattaaa
     1261 gctgcacttg gagtagtatt tccgattcca acctctccga ttgccaatat atttattccg
     1321 gattcgattg cttctgatgc aatttttgca ccggttaaca ggcttcttaa agcttcttcc
     1381 cttgtcatgg caggaccttc ggcaatatta tccgtgccgt tttttatacg atttgccatt
     1441 agatccggag gctcaagacg accacatata cctatatcat tgcatataag ctttgcgcct
     1501 gcatgtccgg ttaatacgtt aataccgcct cctccgttaa ggtaactata atacaattga
     1561 gcagttacct cttgtggcgc tgcactcact ccttctttta caacaccatg gtcccctgcc
     1621 attaaaagta ctgcttttcc cttaacttca gggaattcgg tgccttgtat gcctgccagc
     1681 tgaacggcaa tgtcttctaa aacacccaga cttctaagcg gttttatctt ggcatcaagt
     1741 ctcttctgca ttccttcggc tgaaattgaa tcaggctttt taattccttt tataatttca
     1801 tttaataccg actctaattc tttttcactt gcttctttaa tgttaaaatt cttgtaaaat
     1861 tctacgtgca ttctttacct ccaatttcat atatgtaact ctcatcaaac aataatagtt
     1921 tcccggcaac caacaaaaaa atcccgacat aataaaatta ttatgttcgg gactttacct
     1981 tcatcgccgt ttgctttcaa tcgaaagact tctattcata ggcaggtctc ctgactcaga
     2041 ctcaaacgtc cttttactcc ttcccggcat ataccagtgg tatagtaaag gactaatctt
     2101 cacagtgtcg ggtacgtacc ggattttcac cggtttccct attctccttc ttaggcacct
     2161 atgaatttta ttaatgctat tctaatacaa agattaaacc atgtcaatta ttttaattaa
     2221 cactcaggct aataacagtt ggatgttatc agtaacgctt ttttacattc ctaaattttc
     2281 atttattatt atacactctg cttcaacata tttcatcttt ttaagatgaa tattcagcgt
     2341 gttgcatatt ctttccgaac ttgaacaatc atagcagcgg tcagctttta ccgcacacgg
     2401 agtattataa cccagacgtt ttgaattttg aggagcagca atgtttcttg cacgccagat
     2461 agccttttct aaattaggct cgattttatt tgttccgaaa acaaagtaaa ttttcttatg
     2521 tccaaacagc gaacctgcta cacgatttcc tgttccatct atatttacca gttctccggt
     2581 ttcagctaca ccgttgaccg acgtaaaaaa tacatctgtc agaagcgctt ttttagcaac
     2641 ttcgttaaat tcatctccgg aatatggtgc cgggtctatt actgtattat tttttgataa
     2701 aagctcagct aa
//
LOCUS       DAOSOW010000261         2707 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_37600_length_2707_cov_3.433258, whole genome shotgun sequence.
ACCESSION   DAOSOW010000261 DAOSOW010000000
VERSION     DAOSOW010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37600_length_2707_cov_3.433258"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="PLD67_07020"
     CDS             <1..510
                     /locus_tag="PLD67_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010899484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOT22308.1"
                     /translation="VNYEPSCISIAEWWKQLYGESEGKEGKGIFPASLNFTTDLHSMG
                     QFVQEGREIIIETTLLIENSEDDMVIPFDLNDFDKLNYISGKTFNFVNLKAFEGTFMA
                     HNEGKVPGIIISVPKMDEFYLAKLFYFFMMSCGISGYTNKVDPFTQPGVEAYKKNMFK
                     LLKEEEHTK"
     gene            522..1421
                     /locus_tag="PLD67_07025"
     CDS             522..1421
                     /locus_tag="PLD67_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1-like domain-containing RNA-binding protein"
                     /protein_id="HOT22309.1"
                     /translation="MMKLGEMQELEVAKIVNIGAYLQSEDKEKTEDRVLLPIKQVPAG
                     TKVGDKINVFIYRDSDDKIIATVKKPKITLGEIASLKVVEMSRIGAFLNWGLEKDLFL
                     PFKEQIGDIRLNGEYMVGLYIDKSDRLCATMNLFKVLRTDSPYKVIDIVKGTVFSLKR
                     GLGAMVAVDNKYLGLIHEGEIIKPLHPGQSVEVRVSNIKEDGKLDLSLKDAPRLQIDK
                     DGEKILKVLIRNKGSLPLNDDSSPEEVNKILGMSKSSFKKASGRLMKRRLIIMTKNGI
                     EMLSDDEKQQSKVKKSNIRYKKK"
     gene            1480..2181
                     /gene="deoD"
                     /locus_tag="PLD67_07030"
     CDS             1480..2181
                     /gene="deoD"
                     /locus_tag="PLD67_07030"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOT22310.1"
                     /translation="MSTPHNSAVIGQIAETVLLPGDPLRAKFIAENFLEDVEQFNTVR
                     NMYGFTGSYKGKKVSVMGSGMGCASIGIYSYELIHMYGVKNLIRVGSCGAYAPDLNLY
                     DIVLAIGASTDSNYAYQYELPGIYSATASWELLSKAKKVADDNNIKAVVGNIVTTDIF
                     YHDNSENWRKWAKMGCLAVEMETYALYCNANRAGVNALTILTVSDSLEHKTETTPEQR
                     EKSFTDMIKIALEIL"
     gene            complement(2255..2455)
                     /locus_tag="PLD67_07035"
     CDS             complement(2255..2455)
                     /locus_tag="PLD67_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOT22311.1"
                     /translation="MKTGTVKWFDSAKGFGFITSDEGNDVFVHFSAINADGFKTLEEG
                     QKVTFEVVEGTRGPQAANVEII"
     gene            complement(2602..>2707)
                     /locus_tag="PLD67_07040"
     CDS             complement(2602..>2707)
                     /locus_tag="PLD67_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006081219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOT22312.1"
                     /translation="INSDGFKTLEEGQKVNFDIVDGQKGPQASNVTVA"
BASE COUNT          948 a          441 c          559 g          759 t
ORIGIN      
        1 gttaactatg aaccatcctg catatcaatt gccgaatggt ggaagcagct ttacggcgaa
       61 agtgaaggaa aagaagggaa gggtattttc cctgcttcgc ttaatttcac tacagacctt
      121 cattccatgg gacaatttgt tcaagaggga agagaaataa ttattgaaac aaccctatta
      181 attgaaaata gtgaagatga tatggtaatt ccttttgact taaatgattt tgataaatta
      241 aattatatat cgggaaaaac atttaacttt gtaaacttaa aagcatttga aggaaccttc
      301 atggctcaca atgagggaaa agtaccgggg attattatca gtgttcctaa gatggatgaa
      361 ttttatcttg ccaagctttt ttactttttt atgatgagct gtggaataag tgggtatact
      421 aataaggtag acccctttac tcagccggga gtggaagcat acaaaaagaa tatgttcaag
      481 cttctaaagg aagaggaaca caccaagtga gaggagaaat tatgatgaaa ctgggagaaa
      541 tgcaggaatt agaagttgca aaaatagtaa acataggtgc ctatttgcaa agtgaagaca
      601 aggagaaaac tgaagacaga gtattgcttc caataaagca agttcctgca ggaacaaaag
      661 taggagataa aataaatgta tttatttaca gagactctga tgacaaaatt atagccactg
      721 taaaaaaacc aaaaataaca ttgggtgaaa ttgcatcctt gaaggttgtc gagatgtcaa
      781 ggataggagc atttcttaac tggggtttgg aaaaagactt attccttccc tttaaggaac
      841 agataggcga tataaggcta aatggagaat acatggtcgg cctttatatt gacaagtcgg
      901 acagactttg tgcaaccatg aatttattta aggtattgag aacagactca ccttacaagg
      961 ttatcgacat tgtaaagggt actgtattca gcttaaaaag agggctcggt gcaatggttg
     1021 cggtagacaa caagtattta ggattaatac atgagggaga aattataaag cctcttcatc
     1081 ccggacagtc agttgaggtg agggtcagca atataaagga agacgggaaa ctggatttaa
     1141 gcctaaagga tgctccgagg cttcaaatag acaaggacgg tgaaaaaatc ctaaaggtac
     1201 ttattagaaa caagggctcc ttacctttaa acgatgacag cagtcctgaa gaagttaata
     1261 agattttggg gatgagcaaa agctccttta aaaaggcttc agggcgttta atgaaaagaa
     1321 gattgattat tatgaccaaa aacggcatag aaatgctttc tgatgatgaa aaacaacaat
     1381 ccaaggtaaa aaagtccaat ataaggtaca aaaagaagta gcttagttga caaaagatta
     1441 ttagctgata acctatataa aaaacacaag gaggattata tgtctacacc acacaatagt
     1501 gcagttatag ggcagattgc cgaaacagtc ttgttgcccg gcgacccctt gagggcaaaa
     1561 tttattgcag aaaactttct tgaagatgtt gagcaattta acactgtaag aaatatgtac
     1621 ggttttaccg gaagctacaa aggcaaaaaa gtttctgtta tgggaagcgg aatgggctgt
     1681 gcttcaatag gaatttattc ttatgaatta atacatatgt acggagtcaa aaatttaatt
     1741 cgtgtaggct cctgcggggc ttatgcacct gatttgaatt tgtacgacat agtacttgca
     1801 ataggagcaa gcacagattc aaattacgcc tatcaatatg agctgccggg aatttattcg
     1861 gcaacagctt catgggagct tttatcaaag gctaagaagg ttgcggatga taacaatata
     1921 aaagcagtag tggggaatat tgttactact gatatttttt atcatgataa ttctgagaat
     1981 tggagaaaat gggcaaagat gggctgttta gccgtcgaaa tggaaaccta cgccttgtat
     2041 tgcaatgcca acagggcagg agtaaatgcc cttacaatat tgacagtatc tgattccctc
     2101 gagcataaga ctgaaactac cccggagcaa agagaaaaga gctttacgga catgataaaa
     2161 atagcattgg agatattata acatataatg tgcagcgagc ctgcgagccg gaaaaaaaag
     2221 cacctattct tttataggtg ctttagagta tacattagat gatttctacg ttagcagctt
     2281 gagggcctct cgttccttca accacttcaa aggttacttt ttgtccttct tccaaagtct
     2341 tgaatccatc tgcgtttata gcggaaaaat gaacaaacac atcattccct tcatcgcttg
     2401 tgataaagcc aaaacctttc gcagaatcaa accattttac tgtacctgtt ttcattaaaa
     2461 ctcctccatg ttttttaaaa ataaataata taatgaaaaa gaactacaca tgtagttctt
     2521 atcacgtctc cttcaaatac tatattaata taagtaatgc atttatctat attaatgtat
     2581 tatttcgaaa aacaattact actatgctac agtaacattg ctagcctggg gtcctttttg
     2641 accatcaact atgtcaaagt taactttttg tccttcttcc aaagtcttga atccatccga
     2701 gtttata
//
LOCUS       DAOSOW010000262         2706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_37619_length_2706_cov_3.670690, whole genome shotgun sequence.
ACCESSION   DAOSOW010000262 DAOSOW010000000
VERSION     DAOSOW010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37619_length_2706_cov_3.670690"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PLD67_07045"
     CDS             <1..420
                     /locus_tag="PLD67_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22313.1"
                     /translation="NNIVSLLNISFNSNYTNEIDFRNYNFGNYSFSLYKDTTLFRYLR
                     NMTMPLNINLRVVELSNLLMIEKNPKPTVNMADYENLFTKYSVNKLYVLNFLGEKERA
                     FVEGINDYDIIKTFEMYKKNPSIFDDTYTGILKKVKE"
     gene            complement(475..1683)
                     /gene="pepT"
                     /locus_tag="PLD67_07050"
     CDS             complement(475..1683)
                     /gene="pepT"
                     /locus_tag="PLD67_07050"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOT22314.1"
                     /translation="MKVHERFLNYVKIDTQSVHDAKKIPSSEKQKDLGRLLVEEMISI
                     GIEDAYMDENGYVYGTVKGNTDAPVIGFIAHMDTSPDMPGSGVKPKIIYNYDGSDIML
                     NDEKQIVMKTEMFEHLSKYIDQDLIVTDGTTLLGADDKAGIAEIMSMAEYLINNPQIK
                     HGTIKIGFTPDEEVGQGAELFNIEKFGADYAYTVDGGEIGELEYENFNAASAKVTING
                     VNIHPGTAKNKMKNSILIGMEFHNMLPVYETPENTEGYEGFSLLTDMSGNVEKTLLEY
                     IIRDHDIAKFNIKKERFQKIADYLNEKYGQGTIELKLEDSYFNMKEKILPHMHIVENA
                     IRAMENVGIEPKVVPIRGGTDGARLSYMGLPCPNLCTGGHNFHGRFEYISIQSMEKVV
                     ELLIEIATVK"
     gene            1994..2629
                     /locus_tag="PLD67_07055"
     CDS             1994..2629
                     /locus_tag="PLD67_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014552713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOT22315.1"
                     /translation="MDFIKEGYKVKSATVIKNLEKRNMEGYYCETVEEAVEKAMSMIK
                     QDDTVGWGGSTTIDQIGIKKLLEEKNIAVYDRDKETDPAEKVKMMKKALTSDVFLTSA
                     NAITMDGELLNIDGNGNRLAAYCYGPNSVIVVAGMNKIVTDLDVALKKARLDATVPNT
                     FRTNSKAPCHFTGKCIECTMSDTLCGQILITRFCKPKNRIKVILVGENLGF"
BASE COUNT          837 a          478 c          478 g          913 t
ORIGIN      
        1 aacaatattg tttctctttt gaatatcagc tttaattcaa actatacaaa cgaaattgat
       61 tttagaaatt ataattttgg aaattacagt ttttcattgt ataaggatac aaccttattc
      121 agatacttga gaaatatgac tatgccctta aatattaact taagagttgt cgagctgtca
      181 aatttgctga tgattgaaaa aaacccgaaa ccgacagtaa acatggctga ttacgaaaat
      241 ctgtttacca agtattccgt taacaaattg tatgttctta atttcttggg tgaaaaggaa
      301 agagcatttg ttgagggaat aaacgattat gatattatca aaacatttga aatgtacaag
      361 aaaaatccct caatatttga cgacacctat acagggattt tgaaaaaagt aaaagaataa
      421 gtcatagaag tttggtgcca ggcaccggta agtggtgcct ggcaccaagt gtctctattt
      481 cactgttgca atttctatca acagttccac aaccttttcc attgactgaa ttgatatgta
      541 ttcgaatcta ccgtgaaaat tgtgacctcc ggtacataga ttcggacaag gtaaacccat
      601 gtatgacaat ctagctccgt cggttccacc tcttattgga actacctttg gctcaatacc
      661 tacattttcc atagccctaa ttgcattttc cacaatgtgc atgtgaggga gaatcttctc
      721 cttcatatta aaatatgaat cttctaattt taactctatc gttccttgac cgtatttttc
      781 gtttaagtag tctgcaatct tttgaaatct ttcctttttt atattgaatt ttgcaatatc
      841 gtggtcgcga ataatatatt cgagcagtgt tttttcaaca tttccggaca tatcggttaa
      901 gaggctgaat ccttcgtatc cttcggtatt ctccggagtt tcatatacag gcagcatgtt
      961 gtgaaattcc attccgataa gtatggagtt tttcattttg tttttggcag ttcccggatg
     1021 aatattcact ccgtttattg ttacttttgc acttgcagca ttgaagtttt catactccaa
     1081 ttctcctatt tctcctccgt caacagtata tgcataatca gccccaaatt tttcaatatt
     1141 gaacagctct gccccctgac caacttcctc atcgggagtg aatcctattt ttattgtacc
     1201 gtgtttaatt tgtggattgt tgattaagta ttcagccatt gacattattt cagcaattcc
     1261 tgccttgtca tcggcaccta gaagggttgt gccgtctgtt acaatcaaat cctgatctat
     1321 atatttagat aaatgctcaa acatttcagt tttcattaca atttgttttt catcatttag
     1381 cattatgtcg ctgccgtcat aattatatat tatcttaggt tttacaccgc tcccgggcat
     1441 atcgggcgat gtgtccatat gagcaataaa tcctataaca ggtgcatctg tattaccttt
     1501 aacagttccg taaacgtatc cgttttcatc catgtatgca tcttcgattc ctattgatat
     1561 catttcttca accagcagtc ttcctaagtc cttttgtttt tcactgcttg gaattttttt
     1621 tgcatcgtgt acggattgag tatcaatttt aacataattt aaaaatcttt cgtgaacttt
     1681 cattttgtcc tcgctttatt aattatttta tgatattagt atatcattat ggattttata
     1741 tatcaatatt ctttatgata agaacatacc tgagggtgca gcctggcatt ctgagagctt
     1801 tagctcgaag aatctcatct tataaaacct gagatccttc gcttacgctc aggatgacaa
     1861 tcgcagagta tattcgttta tagtggtgtg gtaatcacat gataattctg aaagctctcg
     1921 ctcgaagaat ctaattgttg aattgaagat taaaataata tataatatgg acaagaatat
     1981 atgggaggtt gttatggatt ttattaaaga aggatataag gtaaaaagtg ctactgttat
     2041 taagaatctt gaaaaaagaa atatggaagg ttactattgt gaaacagtag aagaagctgt
     2101 agaaaaagca atgtcaatga ttaaacagga tgatacagtt ggatggggcg gttcaactac
     2161 tatagaccaa attggaatca agaaactttt ggaagaaaaa aatattgctg tctatgatag
     2221 ggataaagaa actgaccctg cagaaaaagt aaaaatgatg aaaaaggcat tgacttctga
     2281 tgtgttcctt acaagtgcca atgctattac aatggacgga gaacttctaa atattgacgg
     2341 aaacggtaat cggcttgctg catattgcta cggtcccaac agcgttatag ttgttgcagg
     2401 aatgaacaaa atagtaacag atttggatgt tgcgctgaaa aaagcaagac ttgatgcaac
     2461 tgttccaaac accttcagaa caaactcaaa agccccatgc cactttacgg gcaaatgtat
     2521 agagtgcacg atgagcgata cattgtgcgg tcaaattctt ataacaagat tctgcaaacc
     2581 taagaataga ataaaagtaa tattggtcgg tgaaaattta gggttttaag atgccctaac
     2641 cctatttttc agagtctttt ttctgaaaaa tagcggtagg tcattcgcaa cgaattccca
     2701 atttat
//
LOCUS       DAOSOW010000263         2692 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_37891_length_2692_cov_3.245355, whole genome shotgun sequence.
ACCESSION   DAOSOW010000263 DAOSOW010000000
VERSION     DAOSOW010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37891_length_2692_cov_3.245355"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1062)
                     /gene="hflX"
                     /locus_tag="PLD67_07060"
     CDS             complement(<1..1062)
                     /gene="hflX"
                     /locus_tag="PLD67_07060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOT22316.1"
                     /translation="MEKERCLLVAVQLPGEEENDMEELEQLVEAAGGEVISSIVQNKQ
                     SIDNRFYAGQGKIEEIANYVKELDIDTVIFNDELSGSQIRNIEEIVEAKVIDRTNLIL
                     DIFAKRALTKEGQLQVELAQLKYSLPRLIGVNKNLSRLGGGIGTRGPGEQKLEIDRRR
                     IKEKITDIQNQLEVLEKVRETKRKKRMKDQVPIVSIVGYTNAGKSTLLNALMDNEYRE
                     EGENKKVFAHDMLFATLDTELRRVRLPGGRSAVFSDTVGFIKKLPTQIVEAFKGTLEE
                     IKYADLIIHLIDINDENLSAHKETTIELIEKITGSEIPILTVYNKVDKILNEKVSVLN
                     NGIIYISAKYKYNIQELLEA"
     gene            1335..>2692
                     /locus_tag="PLD67_07065"
     CDS             1335..>2692
                     /locus_tag="PLD67_07065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOT22317.1"
                     /translation="MSKEKKNNEINENNSDSINKNDKKKRTAKKKRKVIIRVTLLLIL
                     VAVIIAGGAVLGMVMGIIRNAPEIDPRNVLGTLTESSVIVDENGNVIEQIHDPNENRE
                     IVPLKQIPKYLKDAFIAIEDHRFEDHPGIDIRRIMGAVIHNVREGDPTAQGASTITQQ
                     LVKNLYLTSDKVWERKITEIYLAIKMERVLSKEQILENYLNTIPLGQSQYGVQAASYA
                     YFSKDVSDLTLAESALLAAAAKSTVKYAPFNRYNLEDISDIPEEDIVGNVFIGSVQYA
                     CVYNQNAIDRQHTILNRMLELEYITQEEYDAAMAEDMRVALNPGQTKMEGISSTPMDY
                     VKEKVVEDLIATRGMSYEEAENYLYKGGLTITSTIDVNIQKSLEQSYDNFPVLYLGAE
                     PTGDKPIAQDWRYFRWSGGEGTGMLDAGLNILNESGQLIFFAKENIMDEENNIYLNPD
                     EY"
BASE COUNT          856 a          503 c          463 g          870 t
ORIGIN      
        1 agcttctagc agctcttgaa tattatattt atattttgct gaaatgtaaa taataccatt
       61 gtttaaaaca gatacttttt cattcaaaat tttatccacc ttattataaa cggtaagaat
      121 aggtatttca cttcccgtaa ttttttcaat cagctcaata gtagtttcct tatgagccga
      181 caaattttca tcatttatgt caataagatg aattattaag tcagcatatt tgatttcttc
      241 caatgttccc ttgaaggcct ccacaatttg tgtgggaagc tttttaatga atcctactgt
      301 atcggaaaag acagcactcc ttcccccggg caatctcact cttctaagct ccgtatcaag
      361 ggttgcaaaa agcatgtcat gggcgaaaac cttcttgttt tctccttctt ccctgtattc
      421 attatccatc agtgcattta gcaaggttga tttccctgca ttggtataac ccacaatgga
      481 aactatcggc acctggtcct tcattctttt ctttcttttg gtttccctta ccttttccaa
      541 tacttccaac tgattttgta tatcggtgat tttttctttt attcttcttc tgtcaatttc
      601 cagcttttgc tctcctggtc ctcttgtgcc tattcctccg cctaatcgag acaggttttt
      661 atttacacca ataagtctcg gaaggctgta ttttaactgc gccaattcca cttgaagctg
      721 tccttccttg gttaaggctc tttttgcaaa tatatcaagt atgaggttag tcctgtctat
      781 tacctttgct tcgacaatct cctcaatatt tcgtatttga gaaccactta attcatcatt
      841 gaaaattact gtatcaatat ctaattcttt cacataatta gctatttctt caattttccc
      901 ctgacctgca taaaatctgt tgtcaatact ttgtttgttt tgaacaatgc ttgaaataac
      961 ttcgccgcct gcagcttcaa ccaattgctc caattcttcc atatcgtttt cttcttcgcc
     1021 cggcagttga actgcaacca gcaaacatct ttctttttcc attaaacctc cgtaatatat
     1081 agcaatctaa aaaattcctt tatgtcatcc tgaatatacc ctaagcaatg atactgcggg
     1141 attatatata atataacatt acaaatgtct ttttgcaatt actctttatt tcttacaatt
     1201 ttcataattt ttcctgagta atttttaaac tattgctttc aagctttttc tattatataa
     1261 ttattaagat gaaaatcttt gacaatattt gctttaaatg tggtatacta actaatgcaa
     1321 acgcggaggt agttatgtca aaagaaaaaa agaataatga aataaatgaa aataattcag
     1381 attctataaa taaaaatgac aagaaaaaaa gaaccgcaaa aaagaaaaga aaggttatta
     1441 tcagagtaac ccttctttta atacttgtgg cagtgattat tgccggggga gccgttctgg
     1501 gaatggtgat gggaataata agaaacgctc ctgaaatcga tcctaggaat gttcttggta
     1561 ctcttacgga aagctcagtt atcgtagatg aaaacggaaa tgttattgag caaatccacg
     1621 acccaaacga aaacagagaa atagtaccgc taaaacaaat acctaaatac ttaaaagatg
     1681 cttttattgc aatagaagat catcgttttg aagaccatcc cggtattgac ataagacgta
     1741 ttatgggagc tgttatacat aatgtgcgag aaggagaccc tactgcacaa ggtgcaagca
     1801 cgattaccca acagcttgtt aaaaatttat accttactag cgataaagtt tgggagcgaa
     1861 aaataacgga aatttatttg gctattaaga tggaaagagt tctttccaag gaacaaattc
     1921 tagaaaatta ccttaacacc ataccactag ggcaaagcca atatggggtt caagctgcat
     1981 catatgctta tttttcaaaa gatgtaagtg atcttacctt agctgaaagt gcattgttag
     2041 cagcggctgc aaaaagtacg gtgaaatacg caccgtttaa tcgttataac ttggaggata
     2101 taagcgatat tcccgaagaa gatatagtag gaaatgtatt tatcggctct gttcaatatg
     2161 cctgtgtgta caatcaaaac gcaatagaca ggcagcatac tattttaaac agaatgcttg
     2221 aattagaata cattacccaa gaagaatatg atgctgcgat ggcagaggat atgcgggttg
     2281 cattaaatcc gggacagaca aagatggaag gtatttcatc aacacctatg gactatgtga
     2341 aagaaaaggt tgtggaagac ttaatagcta ctcgaggcat gtcttatgag gaagcagaaa
     2401 actatctgta taaaggcggt ttaaccatta cttctacaat tgatgttaat attcaaaaat
     2461 ccttagagca gtcttatgat aatttccctg tattatatct gggtgccgag cctacaggag
     2521 ataaacctat tgctcaggac tggagatact tcagatggtc aggaggggaa ggaaccggaa
     2581 tgcttgatgc aggattaaat atcctgaatg aaagcggcca gcttatattc tttgccaaag
     2641 aaaatataat ggatgaagaa aacaacattt acttgaatcc tgatgagtac ag
//
LOCUS       DAOSOW010000264         2690 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_37924_length_2690_cov_3.184440, whole genome shotgun sequence.
ACCESSION   DAOSOW010000264 DAOSOW010000000
VERSION     DAOSOW010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37924_length_2690_cov_3.184440"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="PLD67_07070"
     CDS             complement(<1..1439)
                     /locus_tag="PLD67_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOT22318.1"
                     /translation="MIDYNNFESIRIGLASPDKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCEKIFGPTKDWECHCGKYKRVRYKGVVCDRCGVEVTKAKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLILDMSPRALEKVLYFAQYVVTEAGDTSLSEKQLLTEMEYREYKE
                     HYKNAFKAEMGAEAIKKLLEKIDLESEAAELKAQLRDSKGQKKIRITRRLEVIEAFRA
                     SGNRPEWMILDVIPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFNQCGLPKRMALELFKPFVMKKLVETGAAHNIKSAKKKVEK
                     VDTAVWDVLDEIIKDHTVLLNRAPTLHRLGIQSFEPVLVEGKAIKLHPLVCTAYNADF
                     DGEQMAVHVPLSVEAQAETRFLMMSVNNIL"
     gene            complement(1571..>2690)
                     /locus_tag="PLD67_07075"
     CDS             complement(1571..>2690)
                     /locus_tag="PLD67_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOT22319.1"
                     /translation="ERLLRAIFGEKAREVRDTSLKVPHGESGIVVDVKVFTRENGDEL
                     SPGVSKLVRVYIATKRKINVGDKMCGRHGNKGVISMIMPEEDMPFLPDGTPLEVVLNP
                     LGVPSRMNIGQVLEVHLGLAAKKLGWEVATPVFDGAVEDDIWDALEMAGYPRSGKIDL
                     RDGRSGEYFEGPVTVGYMYMLKLHHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHTLQEILTVKSDDVNGRVKTYEAIVKGENIPEPGIPESFKVLVK
                     ELQSLALDIKIITSDDEVEIIETIDNDDDDLPIDSESLGDLALIEGDMEGMQIDSDDI
                     EDIDDIDDLDDLGDIDDIDSDELDELDIEEDFDDEDKF"
BASE COUNT          705 a          655 c          423 g          907 t
ORIGIN      
        1 agaatattat tcacggacat catcaaaaat cttgtttctg cctgtgcctc caccgataaa
       61 ggaacgtgaa cggccatctg ttctccgtca aagtctgcat tatatgcggt acataccaaa
      121 ggatgaagct ttatagcctt tccttcaaca agtacaggct caaaagactg aattccgagt
      181 ctgtgcaaag tcggtgcacg gtttaacaat acggtatggt ctttgattat ttcatccagt
      241 acatcccata cagcagtatc aaccttttca acctttttct ttgcactttt aatattgtgg
      301 gcagctcccg tttcaacaag ctttttcata acaaaaggct taaataattc aagagccatt
      361 cttttcggaa gcccgcactg attaaacttc aactccggtc caacgactat aaccgaacgt
      421 cctgaataat ccacacgctt accgagaagg ttttgcctga aacggccttg cttaccttta
      481 agcatatcag atagtgattt taaaggtctg tttcccggac ctgttacagg tcttcctcgt
      541 cttccgttat ctattaaagc atcaactgct tcctgaagca ttcttttttc atttcttaca
      601 attatatcag gtgctcctaa atcaaggagt ctttttaaac gattatttct gtttataact
      661 cttctgtaca agtcatttaa atctgaagtt gcaaaacgac ctccgtcaag ttgtaccatt
      721 ggtcttagct ccggtgggat gacaggtata acatccaaaa tcatccattc cggacggttg
      781 cctgaagctc tgaaagcttc aattacttcc aatcttcttg taatacgaat ttttttctgt
      841 cccttgctgt ctcttaactg agcttttaat tcggcagctt cgctttccag gtcaattttt
      901 tcaagaagtt ttttaatggc ttctgcaccc atttcagctt taaatgcgtt cttataatgc
      961 tccttgtatt ctctgtattc catttctgtc aataattgtt tttctgacaa ggatgtatcc
     1021 cctgcttccg taacaacata ttgagcaaag taaagtactt tttcaagtgc tcttggagac
     1081 atatcaagaa taaggcccat tcttgaagga atacccttga aataccatat gtgagaaaca
     1141 ggagcagcaa gctcgatgtg ccccattctt tctcttctta cttttgcctt tgtaacctca
     1201 actccgcacc tgtcacaaac tacacctttg tatcttaccc ttttatattt gccgcaatgg
     1261 cattcccagt cctttgtagg accgaagatt ttttcgcaaa aaagtccgtc tttttcaggc
     1321 tttagcgttc tgtaatttat tgtctcaggt ttctttactt ctcctcttga ccattgtctg
     1381 attttatcgg gagaagctaa cccgattctt attgattcga aattattgta atctatcaag
     1441 gagttcgctc ccttcttttt tagtttttaa tcggtacttg ttttgttgtt cgatgtaacc
     1501 aactgtccgg ggacattcct ctatgcctgc tccaaagact tgctctacat agaggtgtat
     1561 ctccattcat ttaaaactta tcttcgtcgt caaaatcctc ttcaatgtcc aactcatcta
     1621 actcatcaga atcaatatca tctatatcac ctaaatcatc taaatcgtca atatcgtcaa
     1681 tatcttctat gtcgtcacta tcaatctgca tgccttccat gtctccttca atcaatgcca
     1741 aatcgcctaa gctttcagaa tcaataggta aatcatcatc atcattatcg atagtttcga
     1801 ttatttcaac ttcatcatca ctggtaataa tttttatatc caaagccaaa ctctgcaatt
     1861 cttttacaag taccttgaaa gattcaggga ttccgggttc aggaatattt tcgcctttta
     1921 cgattgcttc atatgtttta accctgccgt taacatcatc ggattttacc gttaatattt
     1981 cctgcaatgt atgagccgca ccatatgctt ccaaagccca aacttccatt tctccaaatc
     2041 tttgacctcc aaattgtgct tttccgccta aaggctgctg agtaaccaat gaataaggtc
     2101 ccgtggagcg cgcatgtatt ttatcgtcaa caagatggtg aagcttaagc atatacatat
     2161 atcccacggt tacgggtccc tcgaaatatt ctcctgaacg accgtctctt aaatcaatct
     2221 tgccgcttcg cggatagcct gccatttcaa gagcatccca tatgtcatct tcgactgcac
     2281 cgtcaaatac cggcgttgca acttcccagc ccagtttttt ggcagccaat ccaaggtgga
     2341 cttccaaaac ttgaccaata ttcatacgag aaggaacacc aagaggattc aatactactt
     2401 caagcggagt tccgtccggt aaaaacggca tatcctcttc aggcattatc attgaaataa
     2461 cacctttatt tccgtgtctt ccgcacatct tgtcgcctac atttatttta cgttttgtag
     2521 ctatataaac tcttacaagt ttgcttactc cggggcttag ctcatcgccg ttttcccttg
     2581 taaatacttt aacatcaaca acaattccgc tttcaccgtg aggaacctta agggaagtat
     2641 ctcttacttc tcttgctttt tcgccgaata tcgcccttaa aagtctttct
//
LOCUS       DAOSOW010000265         2688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_37956_length_2688_cov_3.131789, whole genome shotgun sequence.
ACCESSION   DAOSOW010000265 DAOSOW010000000
VERSION     DAOSOW010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37956_length_2688_cov_3.131789"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="PLD67_07080"
     CDS             complement(<1..947)
                     /locus_tag="PLD67_07080"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HOT22320.1"
                     /translation="MYYNNFGERRLSMLMDFYELTMANGYFSNGMKDEIVYFDLFFRK
                     NPDGAGFSIVAGLEQIIQYIKELKFYEEDINFLRSKKLFTEEFLQYLKDFKFSGDIYA
                     IPEGTPVFPNEPLITVRAKTIDAQIIETMVLLTINHQSLIATKANRVVRAAKGKPIME
                     FGARRSQGYDGAIYGARAAYIGGVIGTATTLADEAFGVPALGTMAHSWVQMFESEYEA
                     FKSYALNYPDACNLLIDTYNVIKSGIPNAIRISKEVLEPMGKRLKGVRIDSGDIAYLS
                     KKCRKMLNDAGLHDCAIVASNSLDEFIISDLLDQGAKID"
     gene            complement(1089..1898)
                     /locus_tag="PLD67_07085"
     CDS             complement(1089..1898)
                     /locus_tag="PLD67_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOT22321.1"
                     /translation="MVIVKAVAVTGGIGAICGLLLALAAKLFHIDVDQRITDVRNALP
                     GANCGACGYAGCDAMAEAIAAGRAPVDGCPVANYQAHAKIADIMGTSAEEGEKKVAHV
                     LCRGNDCLASKKHEYHGVRDCKAAAAVQGGDKSCDKGCLGYGNCVRVCDFDAIDIIDG
                     IAVIDKEKCTACGKCIEECPKAVIEFVPYKNNVIVGCNNRDFGKAVKDVCKIGCIACK
                     ICEKNCPYDAIHVTNNLAKVDYDKCTHCMICVEKCPTKVIEGDLSIKDKAV"
     gene            complement(1910..2485)
                     /gene="rsxA"
                     /locus_tag="PLD67_07090"
     CDS             complement(1910..2485)
                     /gene="rsxA"
                     /locus_tag="PLD67_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOT22322.1"
                     /translation="MELVSLFITSFIVNNIIFMQFLGTCPFLGVSKKIDTAAGMGIAV
                     IFVITLASVITYIIHYAILVNLELEYLQTIVFILIIAALVQFVEMFLKKSAPGLYQAL
                     GIFLPLITTNCAVLGVAINNIIYEYNFVESLVYSVGISAGFLLAIVLFAGVRDKVDYA
                     DLPESLKGSAISLITAGLMSVAFLGFSGLQL"
     gene            complement(2496..>2688)
                     /locus_tag="PLD67_07095"
     CDS             complement(2496..>2688)
                     /locus_tag="PLD67_07095"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HOT22323.1"
                     /translation="GYLVAMILLSSVREILGNGTILGFRILPEAYEPMMMAIQPPGAF
                     ILLGLLIGIVNYLSRRKKA"
BASE COUNT          821 a          599 c          371 g          897 t
ORIGIN      
        1 taatctattt tagctccttg gtccaataaa tcagaaataa taaattcatc cagactgttt
       61 gatgcaacaa ttgcacagtc atgaagccct gcatcattta acatcttcct gcatttcttg
      121 cttaagtatg caatatcgcc gctgtcaatt ctaactcctt taagtctttt gcccataggc
      181 tccaacactt ctttggatat cctgattgca tttggtattc cgctttttat aacgttgtaa
      241 gtatcaatca gtaaattgca cgcatcagga taatttagtg catatgattt aaatgcttca
      301 tactcgcttt caaacatttg cacccatgaa tgagccatgg ttcccagtgc cggaactcca
      361 aaggcttcat ctgccaatgt tgtggctgtt cctattactc cgccgatata agcagctctt
      421 gctccataaa ttgctccatc atagccttga cttcttcttg cgccaaattc cattattggc
      481 ttaccttttg ctgctctaac cactcgattt gctttagtgg caattaagct ttgatgattt
      541 attgttaaaa gcaccatggt ttcaattatt tgagcatcaa ttgtcttggc tcttaccgta
      601 attaaaggct cattgggaaa tacaggagtc ccttcaggta ttgcatatat atcacctgaa
      661 aatttaaaat ctttcaaata ctgcaaaaat tcttccgtaa ataatttttt gcttcttaga
      721 aaattaatat cctcttcata aaattttaat tcttttatat attgaattat ttgctctaat
      781 cctgcaacta ttgaaaaccc tgcaccatca ggattttttc tgaaaaataa atcaaaatat
      841 actatttcat ccttcattcc gttagaaaag tatccgtttg ccattgtaag ttcataaaaa
      901 tccatcagca tagataatct tctttcgcca aaattattgt aatacataat atactcctgt
      961 ttttatcttc ctatggttta ttttatcaca gattatacat tttgtatatt atagtgccaa
     1021 aatcattttt tgtcaaaaaa tatatctacc cattgattct caatcgctga gtagatatta
     1081 tatttgctct atactgcttt atcttttatc gataaatctc cttctattac ctttgtagga
     1141 cacttttcta cacaaatcat gcaatgcgta catttgtcat aatctacctt ggctaaattg
     1201 tttgtaacat gtattgcatc gtaaggacag ttcttttcac agattttgca tgctatacaa
     1261 ccgattttgc aaacatcttt gactgctttt ccgaagtctc tgttgttgca tcctacaata
     1321 acattattct tatacggaac aaattctatt acggcttttg gacattcttc aatacatttt
     1381 ccgcaagcag tacatttttc tttgtctatt actgctattc cgtcaattat gtcaatagca
     1441 tcaaaatcgc atactctgac acagtttccg taaccgaggc atcccttatc acaggactta
     1501 tctccgcctt gaacagccgc tgctgcctta caatccctta ctccatgata ttcatgtttt
     1561 tttgaagcca ggcagtcatt tccacgacat aaaacatgag ctaccttttt ttctccctct
     1621 tcagctgaag tacccataat atcggctatt tttgcatgag cctgataatt tgccaccgga
     1681 catccgtcta caggagctct tccagctgct attgcttcag ccatagcatc acagcctgca
     1741 tatccacagg caccgcaatt tgcacccggc aaagcatttc ttacatcggt aattctttga
     1801 tctacatcta tatgaaacag tttagctgca agagccaata ataatccgca tatggcacct
     1861 atgccgccag tgactgctac tgctttaact ataaccatct ttttacctcc tataactgca
     1921 atcctgaaaa tccaaggaaa gcaactgaca taagccctgc agtaatcaat gaaatagcag
     1981 agccttttaa agattcaggc aaatcagcat aatcgacctt atctctaact cctgcaaaca
     2041 atactattgc cagcaagaaa cctgcggata tacctacaga ataaactaat gattcaacaa
     2101 aattgtactc ataaataata ttatttatgg caacacctaa gactgcacag tttgtagtaa
     2161 taaggggcag gaaaattccc aacgcctgat acaaaccggg agctgatttc ttaagaaaca
     2221 tttcaacaaa ttgtaccaat gcagcaatta tcaatatgaa tactattgtc tgcaaatatt
     2281 ccaattccaa attaaccaaa atagcataat gaataatgta tgttattacg gatgccaagg
     2341 ttataacgaa tataaccgca atacccatac cggctgcagt atcaattttc tttgatactc
     2401 cgaggaaagg acatgttccc aaaaactgca tgaatataat attattaact ataaatgatg
     2461 ttataaataa actaactaat tccattccct acctcctaag ctttctttct tcttgaaaga
     2521 tagtttacta tacctatcaa caacccaagg agaataaatg ctcccggcgg ttgaattgcc
     2581 atcatcatag gctcataagc ttccggaaga attctaaatc ccaaaattgt accgttgcct
     2641 aatatttctc ttacagagct gagtaaaatc atagcaacta agtatcct
//
LOCUS       DAOSOW010000266         2679 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_38112_length_2679_cov_5.933689, whole genome shotgun sequence.
ACCESSION   DAOSOW010000266 DAOSOW010000000
VERSION     DAOSOW010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38112_length_2679_cov_5.933689"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLD67_07100"
     CDS             <1..234
                     /locus_tag="PLD67_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22324.1"
                     /translation="EALETEKQRLQNEIADFSKKVTLLEDKIKLQDEELVAKNQEIQI
                     LKAQNIEKIEGDTVITDEGAAQIDNETSEVESQ"
     gene            206..2119
                     /gene="recJ"
                     /locus_tag="PLD67_07105"
     CDS             206..2119
                     /gene="recJ"
                     /locus_tag="PLD67_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOT22325.1"
                     /translation="MKPVKLKVNEYNNDEIERISKRFNLSKTSAKILLNRNIKSFDDI
                     EQFLEPNFKYFERAENYKDLHKGCKRVIEAINNNDRILIYGDYDVDGVTSISQFVVFL
                     KKAGAYVNYYVPERESEGYGISSDFVNSVKSGEINFELLITVDCGIAEIEKIDEITKL
                     DKDVIIIDHHESREELPNAYAIINPKQKDCPSENKHLCAAGLSFKFLINLNKFLKIDN
                     IEDVLLEYACLGTIADIVDLVKDNRIIASQGLKKINKTKIIGLKKLIEVSGINGEIKS
                     YHIGFILAPRINASGRMDTAKKAIKLMLTKDEEEAEKLASELDSLNNARKEAENIIYK
                     EAIKKIESDFLYKNNVMVVYGDNWHEGVLGIVASRLTEKYYKPCVVISVKDGIGKGSA
                     RSVEHVDIFESFKHTDIYLEKYGGHKLAAGLTILEKNINSFTNELNNYIGSTTDDEYN
                     QIRADAILNISDINLRLYDEINRFEPFGSGNPKPLLALRDVSIKNIRKVGKEGKHISF
                     MLSDGDRHIPVIGFCKIGILEKILTMPQSYIVSLSENIYNGSRSLQLFLHDVEEHEEF
                     DYKIDVNKLKALEFLINKTKSKIIKTDLFILVEKLNKRYNTKITAEEIICMLKKADNI
                     QFALKNDILYIKK"
     gene            2136..2654
                     /locus_tag="PLD67_07110"
     CDS             2136..2654
                     /locus_tag="PLD67_07110"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003470557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOT22326.1"
                     /translation="MDLKEKIRVIEDFPAKGISFKDITTLLKDSEALHECVKEMAERF
                     KDVHVDIIAGPESRGFIFATPLAYLLKTGFVPVRKPGKLPAETIKYEYQLEYGTDSLE
                     IHKDAIKKGQKVLIIDDLLATGGTMYATAKLVEKLGGEVAGLGFLIELKDLKGREKLK
                     EYRVESLITYDK"
BASE COUNT         1045 a          364 c          537 g          733 t
ORIGIN      
        1 gaggctcttg aaaccgaaaa acaaagactt cagaatgaaa tagccgattt cagtaaaaaa
       61 gttacactgc ttgaagacaa aattaaattg caggatgaag aattagttgc taaaaaccaa
      121 gaaatacaaa ttttaaaggc tcaaaatatt gaaaaaattg aaggggatac ggtaattaca
      181 gatgaaggtg ctgcacagat agacaatgaa accagtgaag ttgaaagtca atgaatataa
      241 taatgatgaa atagaaagaa tcagcaaaag gtttaatctt tcaaaaacat ctgcaaaaat
      301 acttttaaac agaaatataa aatcttttga tgatatagag caatttcttg aaccgaactt
      361 caaatatttt gaaagagctg aaaattacaa agatttacat aagggctgca aaagagttat
      421 tgaagcaatt aataataatg acaggattct gatttacggc gactacgatg ttgatggagt
      481 tacttccatc agccagtttg ttgttttttt aaaaaaagca ggtgcttatg tcaattatta
      541 tgttccggaa agagaaagcg aaggatatgg aataagctcc gattttgtta acagtgttaa
      601 aagcggtgaa ataaattttg agcttctgat aaccgtggat tgcggcatag ctgaaattga
      661 aaaaattgac gaaattacaa agcttgataa agatgtaatt ataattgacc atcatgagag
      721 cagggaagag cttcccaatg cttatgccat aattaatccg aagcaaaaag attgtccgtc
      781 cgaaaacaag catttatgtg cagccgggct atcttttaaa tttttgataa atttaaataa
      841 gtttttaaaa atagataata ttgaggatgt gctcctggaa tatgcatgtc ttggcactat
      901 tgccgacata gttgatctgg taaaggacaa caggattatt gccagtcaag gtttaaaaaa
      961 aataaataaa actaagatta tagggcttaa aaaattaatt gaagtatcag gcataaacgg
     1021 agaaattaaa tcctatcata ttggttttat acttgcccca aggattaatg cctcaggaag
     1081 aatggatact gcaaaaaaag caattaagct catgcttaca aaagatgaag aagaagcaga
     1141 aaagcttgca tctgagctgg acagtttaaa caatgcaaga aaagaagcag aaaatataat
     1201 atataaagaa gcaataaaga aaattgaatc tgatttcctg tataaaaata atgtaatggt
     1261 agtatacggg gataattggc atgaaggtgt gcttggcatt gttgcctcaa gacttactga
     1321 aaaatattat aagccctgtg ttgttatatc cgtaaaggat gggataggaa aaggttcagc
     1381 tcgaagtgta gagcatgttg atatatttga atcatttaag catacagata tttatctcga
     1441 aaagtatggc ggccataaat tagcggcagg tcttaccatt ttggaaaaaa atattaattc
     1501 cttcacaaat gaactgaaca attatatcgg ctccactact gacgatgaat ataatcaaat
     1561 tagagcagat gcaattctga atatatcaga cataaattta aggctttatg atgaaataaa
     1621 cagatttgag cctttcggtt caggcaatcc gaaacccttg ctagccctaa gagatgtgtc
     1681 gataaaaaat ataaggaaag tgggcaaaga agggaaacat atatccttta tgctgtctga
     1741 cggagacagg cacattccgg taatagggtt ctgcaaaatc ggaatacttg aaaaaatact
     1801 taccatgcct caatcctaca ttgtatcttt aagtgaaaat atatataacg gcagcagaag
     1861 cttacagctt tttttacatg acgtagagga gcatgaagaa tttgattaca aaattgatgt
     1921 taataaactg aaagcattgg agtttctgat taacaaaaca aaatccaaaa taattaagac
     1981 ggatttattt atacttgtag aaaaacttaa taaaagatat aatacaaaaa taacagcaga
     2041 ggaaataata tgcatgttga aaaaggctga taatattcag tttgctttaa aaaatgacat
     2101 attgtatata aaaaaataaa tgcttggagg aagaaatgga tttaaaggaa aaaattcgtg
     2161 ttatagagga tttcccggct aaggggatta gttttaaaga cattacaacc ttgctgaaag
     2221 attctgaagc tctgcatgaa tgcgtcaagg aaatggcaga aaggtttaaa gatgttcatg
     2281 tcgacataat tgcaggaccg gaatcaagag gctttatatt tgcaacacct cttgcatatt
     2341 tattaaaaac aggatttgta cctgtaagaa agcccgggaa actgccggca gaaactatta
     2401 aatatgaata tcaattggaa tacggcacag attctttgga aattcataag gatgctatta
     2461 aaaaaggaca gaaggtatta attattgatg acttgttggc taccggagga accatgtacg
     2521 ccactgcaaa actggttgaa aagttaggcg gcgaagttgc cggacttggt tttctgatag
     2581 agctgaagga tttaaaagga agagaaaaac taaaagaata cagggtggaa tcattaatca
     2641 catatgataa ataaaactgc gagactgcct gcggataag
//
LOCUS       DAOSOW010000267         2674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_38207_length_2674_cov_3.221840, whole genome shotgun sequence.
ACCESSION   DAOSOW010000267 DAOSOW010000000
VERSION     DAOSOW010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38207_length_2674_cov_3.221840"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..473)
                     /gene="rpsK"
                     /locus_tag="PLD67_07115"
     CDS             complement(75..473)
                     /gene="rpsK"
                     /locus_tag="PLD67_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOT22327.1"
                     /translation="MAAKKQKTTRVRRRERKNIEKGQAHIKSTFNNTLVTLTDLQGNA
                     ISWASSGQLGFKGSRKSTPYAASMVAEEAAKKAMEHGLKTVEVYVKGPGSGREAAIRS
                     LQAAGLEVNLIKDVTPIPHNGCRPPKRRRV"
     gene            complement(489..857)
                     /gene="rpsM"
                     /locus_tag="PLD67_07120"
     CDS             complement(489..857)
                     /gene="rpsM"
                     /locus_tag="PLD67_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOT22328.1"
                     /translation="MARIAGVDLPRDKRVEIGLTYIYGIGRPTSIKILKATGINPDTR
                     VKDLTEDEIQKLRTEIDKHPVEGDARREVALNIKRLKEINCYRGLRHKKGLPVRGQNT
                     KNNARTRKGPKRVSGKKPKK"
     gene            complement(904..1017)
                     /gene="rpmJ"
                     /locus_tag="PLD67_07125"
     CDS             complement(904..1017)
                     /gene="rpmJ"
                     /locus_tag="PLD67_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOT22329.1"
                     /translation="MKVRPSVKPLCEKCKIIRRKGKVMVICENPRHKQKQG"
     gene            complement(1030..1248)
                     /gene="infA"
                     /locus_tag="PLD67_07130"
     CDS             complement(1030..1248)
                     /gene="infA"
                     /locus_tag="PLD67_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOT22330.1"
                     /translation="MSKKDVIEVEGKVLEALPNAMFIVELQNGHQILAHISGKLRMNY
                     IKILPGDTVAVELSPYDLTRGRITWRKK"
     gene            complement(1252..1545)
                     /locus_tag="PLD67_07135"
     CDS             complement(1252..1545)
                     /locus_tag="PLD67_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing RNA-binding protein"
                     /protein_id="HOT22331.1"
                     /translation="MEETTDLKIGQIVKSKAGRDKGRIFLICEVVDEQFVLVCDGDLR
                     KLNNPKKKKVKHLMIYKTVLTEFAEKLQRNENLNDAYVKKLLEPYVSKEMEVE"
     gene            complement(1556..2302)
                     /gene="map"
                     /locus_tag="PLD67_07140"
     CDS             complement(1556..2302)
                     /gene="map"
                     /locus_tag="PLD67_07140"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOT22332.1"
                     /translation="MIILKTRREIEIMKKAGRLVAQSLELVRRHIKPGVTTKELDRLV
                     EDFLKSKNAIPTFKGYNGFPFSICASVNEEVVHGFPSERELREGDIVSIDIGATFEGY
                     VGDSAKTFLVGEVDEEKRHLVEATRQCFYEGIKYARSPYRLSDISHAIQAYAESQGLS
                     VVRDYVGHGVGKNMHEAPQVPNFGKPNKGPRLQEGMVLAIEPMINAGAYNVKVLENNW
                     TVVTVDGKPSAHYEHTVAITDGEPELLTVL"
     gene            complement(2304..>2674)
                     /locus_tag="PLD67_07145"
     CDS             complement(2304..>2674)
                     /locus_tag="PLD67_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOT22333.1"
                     /translation="ADALEDALESMGQKLDYVINIMVRKELLVERAVGRRICKDCGQT
                     YHMTFNKPSKEGVCDNCGGVLLQRKDDTKETVENRINVYMEQTEPLIDYYTKKGIIIN
                     IDGEKPIEQVGKDIISKLRK"
BASE COUNT          627 a          632 c          387 g         1028 t
ORIGIN      
        1 aactttaaac cttctcttct gcatattctg caaacaggcc cggtatatct tgccatcttt
       61 tcacctcgtt tctattatac tctcctgcgc tttggcggtc tgcaaccatt gtgcggtatt
      121 ggagtaacgt cttttattaa atttacttca agtccggctg cctgcagtga acgaatcgct
      181 gcttctcttc ctgagcccgg tcctttaaca tatacctcta ctgttttcaa tccatgctcc
      241 atagcttttt ttgctgcttc ttcagcaacc atgcttgccg catatggtgt agattttctt
      301 gaacccttaa atcctaattg accggaactt gcccatgaaa ttgcatttcc ctgcaagtcg
      361 gtaagagtaa caagtgtatt gttaaaggta gacttaatat gagcctggcc tttttctata
      421 tttttacgtt ctcttcttct tactcgagtt gttttttgtt ttttagctgc cacttaagtc
      481 cctccttttt attttttagg ttttttacct gaaactctct taggaccttt tcttgttctt
      541 gcattatttt tagtattttg tcctcttaca ggcaatcctt ttttgtgtct taatcctcgg
      601 tagcagttga tttctttaag tcttttaata ttcaacgcta cttctcgtct cgcatctcct
      661 tctacgggat gcttatctat ttcagttctt aacttttgaa tttcatcttc cgtcaaatcc
      721 ttaacccttg tatcagggtt gatacctgtt gcttttaaaa ttttgataga agttggtctg
      781 cctattccgt aaatatatgt taagccaatt tcaactctct tatctcttgg caaatcaaca
      841 cctgcgattc tggccattaa gctacacctc ctacattcgt agttttaata tttaatttat
      901 ccattaacct tgtttttgtt tgtgccttgg attttcgcaa attaccataa cctttccttt
      961 tcgtctgata atcttgcact tttcgcacaa aggttttact gatggtctta ctttcattgt
     1021 aatacctcct tactttttcc tccatgttat tcttcctctt gttaaatcat atggtgataa
     1081 ttcaacagct actgtgtctc ccggaagaat cttgatgtag ttcatcctca gttttccaga
     1141 aatatgcgct aaaatctggt gtccgttctg aagctctact atgaacattg catttggaag
     1201 tgcttctaag actttacctt cgacttctat tacatctttc ttggacattg atcactcaac
     1261 ctccatttct tttgatacat acggctctaa taattttttt acatatgcat cgtttagatt
     1321 ttcatttctc tgtaattttt ctgcaaattc tgtcaatact gttttataaa tcattaaatg
     1381 cttcactttc ttctttttag ggttatttaa ttttcttaaa tccccgtcac ataccaacac
     1441 aaattgctca tcaacaacct cgcatataag gaatattctt cccttatctc tgcctgcttt
     1501 tgatttaact atttgaccga ttttcaaatc tgttgtctct tccattagcc cacctctaca
     1561 acacggtcag caattccggc tctccgtctg ttattgcaac tgtatgctcg taatgagcgg
     1621 atggtttgcc gtcaacagtg accactgtcc agttgttttc caaaacctta acattgtatg
     1681 ctcctgcatt tatcataggc tcaatagcaa ggaccattcc ttcctgcagc cgcggacctt
     1741 tattaggttt tccgaagtta ggtacttgcg gtgcttcatg catatttttc cctactccgt
     1801 gcccaacata atctcttact accgataaac cctgtgactc tgcataagct tggattgcgt
     1861 gtgatatatc agacaaacgg tatggacttc gtgcatattt tatgccttca taaaagcact
     1921 gtcttgttgc ttcgactaaa tgcctttttt cttcatccac ttcccccact aaaaatgtct
     1981 ttgcactgtc acccacatat ccttcaaatg ttgcacctat atctatactt actatatctc
     2041 cttcccttaa ctccctttct gaagggaatc catgcactac ttcttcatta acagaagcgc
     2101 atatggaaaa gggaaatccg ttataacctt taaatgtagg tatggcattt ttagatttta
     2161 agaaatcctc aaccaaccgg tcaagctctt tggtggtaac accgggttta atatgccttc
     2221 tcaccaattc gagagactgt gctaccaaac gtccggcctt tttcattatt tcaatttctc
     2281 ttcttgtttt gagaataatc atactatttc ctcaacttag aaataatatc tttgccaact
     2341 tgttctatgg gtttctcacc gtcaatattt attattatac cctttttggt gtaataatca
     2401 ataagcggct ctgtttgctc catataaaca ttaattctgt tttcaacggt ttctttggta
     2461 tcatccttgc gttggagaag cactccgccg cagttgtcac aaactccctc ttttgaaggt
     2521 ttgttaaatg tcatatgata tgtctgaccg caatccttgc aaattcttct gcctacagct
     2581 ctttcaacaa gaagttcttt tctcaccatt atatttatta catagtccag cttttgaccc
     2641 atactttcta aagcatcctc taatgcatct gcct
//
LOCUS       DAOSOW010000268         2665 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_38368_length_2665_cov_4.316858, whole genome shotgun sequence.
ACCESSION   DAOSOW010000268 DAOSOW010000000
VERSION     DAOSOW010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38368_length_2665_cov_4.316858"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..2463)
                     /locus_tag="PLD67_07150"
     CDS             complement(142..2463)
                     /locus_tag="PLD67_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOT22334.1"
                     /translation="MKNMLCSICKKNVAVIYVNKIVEGKMQTTGLCIPCAKKQGIAPM
                     DQIMRQSGMKPEDFDNINKQLTEIIGDMDLEKLSGDMSESSDGINSLMNLMNNAFTGN
                     TSSTSNEFIPEDEDADYTGPGSGTGPKIKEKVKRKKLKYLDTYGLNLTEQARFGELDR
                     IIGREREIDRVVQILNRRSKNNPVLIGEPGVGKTAIAEGLAVRIAEKKVPVKLFNTEI
                     YLLDLTAVVAGTQFRGQFEARMKAIIDEAKKAGNIVIVIDELHNIVGAGEAEGGAMNA
                     ANILKPALAKGDVQIIGATTLDEYRKHIEKDTALERRFQPVIVDEPSIEDSIEIIKGI
                     KDYYEQFHNVKITDEIIRQAVLMSERYITDRYLPDKAIDVIDEAGSRANLKNKGLVEL
                     EALKAELSRIRDEKEEAAENDDYERAAELKVQEIRIESKLKELSNEYENILLTEEDIA
                     YVIEAWTKIPVQRISEAEADKLIHLEERLHRRVVGQHPAVTSLAKAIRRNRSGFKKKK
                     KPSSFIFVGPTGVGKTELARTLAYELFENEDAMIRLDMSEYMEKHTVSKLIGAPPGYV
                     GFDQAGFLTEKVRRKPYSVILLDEIEKAHEDVFNILLQILEDGRLTDNQGRTVNFENT
                     VIIMTSNAGTSIKANRIGFGNTEYEAMESKVKDALKQAFRPEFLNRIDDIIVFSELTK
                     EELSQIVDLMLKDVIKEAEEKKITLTVTDKMKEHILEKGYNTKYGARPLRRAIQKYVE
                     DEISEAYLKGVFKENSLVTVDVNDEGKTVLTII"
BASE COUNT          708 a          579 c          391 g          987 t
ORIGIN      
        1 aaaaataaaa ggtgaacttc ctttggagaa gtgttcccct ttattttttt tgtcattctg
       61 aacgcagtga agaatctcat gagttccttc gctcaaacac aggatgccaa aacgtatcag
      121 cggatagcaa aatgcaggtg actaaataat cgtcagtaca gtttttcctt catcatttac
      181 atcaacagta accaatgaat tttccttaaa tacacctttt aaatatgctt ccgagatttc
      241 gtcttcaaca tacttttgta ttgcccgcct caaaggtctt gcgccatact ttgtattata
      301 gcctttttca agtatatgtt ctttcatttt atcggtaacc gtcaaagtaa ttttcttttc
      361 ttcagcttct ttaataacat ccttaagcat taaatcaaca atctgactta attcttcctt
      421 ggttaattcg ctaaatacta ttatatcatc gatacgattc aagaattcag gcctgaatgc
      481 ttgtttcaga gcatccttta ccttggattc cattgcttca tattctgtgt tgccaaaacc
      541 gattcggtta gccttgatag atgttcctgc atttgaagtc ataattatta ccgtattttc
      601 aaagtttacg gttcttcctt gattgtctgt aagccttccg tcttccaata tctgcagcaa
      661 tatattaaat acatcttcat gagccttttc aatttcatcc aataaaatta ccgaatatgg
      721 ttttcttcgc actttctcgg ttaagaatcc tgcctggtca aagccgacat atccgggagg
      781 agctcctata agtttggaaa ccgtgtgttt ttccatatat tcggacatat caagccttat
      841 cattgcatct tcattctcaa acagttcata agccaaggtt cttgcaagtt cggtcttacc
      901 cacacctgta ggacctacaa atataaatga agatggtttt ttctttttct taaatcccga
      961 acgatttctt cttatagcct ttgcaaggct tgtaactgca ggatgctgac ctacaactct
     1021 cctgtgaagt ctttcctcta aatgtattag tttatcggct tcagcttcgc ttattctctg
     1081 aacgggaatt ttagtccaag cttcaataac atacgcaata tcttcttcag ttaaaagaat
     1141 attctcgtat tcattggata actcttttaa ttttgattca attctgattt cttgtacctt
     1201 aagctctgca gctctttcat aatcatcatt ttctgcagct tcttcttttt cgtctcttat
     1261 tctgctaagc tctgctttta aagcttcaag ttcaaccaat cctttgttct ttaaatttgc
     1321 ccttgaaccg gcttcatcta taacatcaat tgctttatca ggaaggtatc tgtcggtaat
     1381 atatctttca gacattaaaa ctgcctgcct aataatttca tcggttattt ttacattgtg
     1441 aaactgctca taataatctt ttattccttt tataatttcg atggaatcct ctatggaagg
     1501 ttcatctact attacgggct ggaatcttct ttcaagtgca gtatcctttt caatatgctt
     1561 cctgtactca tccaatgttg tggcaccgat aatttgaaca tcacccttgg caagtgccgg
     1621 tttaagtata ttagcggcat tcatagctcc gccttcagct tctcccgcac ctacaatatt
     1681 atgaagttca tcgattacaa taactatatt gcctgctttt ttggcttcat caataatagc
     1741 tttcatgcga gcttcaaatt gacctcgaaa ctgtgtgcca gccacaacgg cagttaagtc
     1801 aagcaggtaa atttcggtgt tgaaaagttt tacgggaact tttttttcgg caattcttac
     1861 cgctaatcct tcagctatcg cagtcttgcc tacacccggc tcaccgatta aaacgggatt
     1921 gtttttagag cgtctgttta atatttgcac aactctgtca atttctctct ctctgcctat
     1981 tatcctgtct aattcgccaa atcgtgcctg ctctgtcaaa ttcaatccat aagtatcaag
     2041 atattttaat ttttttcgtt ttactttttc ctttatttta ggacctgttc cggaacccgg
     2101 cccggtatag tctgcatcct catcctccgg aataaattca ttgcttgtag agctggtgtt
     2161 tcccgtaaaa gcattattca ttaaattcat taaagaatta atgccgtcac tgctttcaga
     2221 catatctccg gatagttttt ctaaatccat atctcctatt atttcagtaa gctgtttgtt
     2281 tatattatca aagtcctcag gtttcatgcc gctttgtctc attatctgat ccataggtgc
     2341 tataccctgc tttttagcgc aaggaatgca caaacctgta gtctgcattt taccttcaac
     2401 aattttatta acatatatta ctgctacatt ctttttacat attgagcaca acatattctt
     2461 catctccttt cctcgaggac attccttttt acccgtcctc attaggagcc gggtataccg
     2521 acagtattga ttataatata atattcttaa aaatgcatta aaaatttgta gaaaaaacta
     2581 tttgcaagaa ttgcttgaaa ttttgtccat atttacaagc tactgccatt ataaaaaata
     2641 taatattatg cttgacttgt aatgt
//
LOCUS       DAOSOW010000269         2655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_38562_length_2655_cov_3.190000, whole genome shotgun sequence.
ACCESSION   DAOSOW010000269 DAOSOW010000000
VERSION     DAOSOW010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38562_length_2655_cov_3.190000"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PLD67_07155"
     CDS             complement(<1..312)
                     /locus_tag="PLD67_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOT22335.1"
                     /translation="MMKKYLDINPEVKTALEQGKPVVALESTIISHGMPYPKNVETAL
                     NVEKIIRDKGAVPATIAILSGKLKVGLTEEEIEYLGKAPKVIKASRRDIPYIVSKKLD
                     GA"
     gene            complement(367..2034)
                     /locus_tag="PLD67_07160"
     CDS             complement(367..2034)
                     /locus_tag="PLD67_07160"
                     /inference="COORDINATES: protein motif:HMM:NF019450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HOT22336.1"
                     /translation="MKTRIFIILILTLLMTVAPFVTEDVYGAEYVKGQSIVAEDQEKA
                     SRGLEFPGLDITWLDIGDSHIQSYSQMDIFSEGIFTLRLSGQTICFDTTGKIIAVGDY
                     NGIHEFSDGMAKVYKYIPRDEPDVPGRLMAPPGQMEGFIDSEGNEVIPLGMHSNMGDM
                     FHEGFTTIGGYEENKGFINKTGEIVIPQIYKDAGNFSEGLAAVQDIETELWGYINKDG
                     DIVIPMLYDEAKPFREEVAYVVKNGMAGYIDKEGNIIIDFMLMPETDKYVDNSFYGGL
                     AVAWDSSGKYGYINKNGDYVLPVKYKEANPFIGEAVYVVIENQNYPNGYGSSFLINRD
                     GERLTPLWQYGRYAGEYMRDGLIRALSTYGPSPKEFIVMLNKYGAEVIPASLNIQYLS
                     SFNEGYALIIADNNGGTAIGLVELPENIDAYKDRKLIRVFIDDKLLNFEDTDPIIENS
                     RTLVPMRAIFEALGAEVEWDDTNKTAIARKDNTTISLKIDDNTAYINNEPVELDVPAK
                     IKNSRTLVPVRFIAESLNADVKWNDEGRAVLIKTSGIDNTAVDDYQE"
     gene            complement(2071..>2655)
                     /locus_tag="PLD67_07165"
     CDS             complement(2071..>2655)
                     /locus_tag="PLD67_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22337.1"
                     /translation="VIYVGATLATFAAFFMISSAQQLPPNKGDINIAEFAENYNYYAK
                     LYKIDFGNEYLDEDGKWAEKETDGTVIYINLTEKPEYKFTIENGYVTGVSFDIEMKNH
                     DYMISSYDKHMFLASLALAGAQNEMGLFSKIPKRIARSIENNILNDYQFVEAGIAFNC
                     DIEHSGYNKITNILVPSENTKENYFSLKFSMNKE"
BASE COUNT          734 a          563 c          373 g          985 t
ORIGIN      
        1 agccccgtct aattttttgg aaacaatata tggaatgtct cttcttgatg ctttaattac
       61 cttaggtgct tttcccaagt attctatttc ttcttcagtg agtccgactt tcaatttgcc
      121 gcttaaaatt gctattgtgg caggcacggc tcccttatcg cgtataatct tttcgacatt
      181 aagtgctgtt tcaacattct taggatatgg cataccgtgt gatataattg ttgattctaa
      241 tgctacaaca ggttttccct gttctaatgc tgttttcact tccggattaa tatctaaata
      301 ctttttcatc ataattcaac tccttcatcc ctccaacatt ccatctattc caccggtttc
      361 tagaatttat tcttggtaat catcaactgc agtgttatct atgcccgatg ttttgataag
      421 aacggctctt ccttcatcat tccattttac atctgcattt aagctttctg caatgaatct
      481 aacaggtact aatgttcttg aatttttaat ttttgcagga acatctaact ccaccggctc
      541 attgttaata tatgctgtat tatcgtcaat ttttaaagat atggttgtat tatctttcct
      601 agcaatagct gttttatttg tatcatccca ctcaacttcc gctccaagag cttcaaatat
      661 tgctctcatc ggcacaaggg ttcttgagtt ttctataatt ggatctgtat cctcaaaatt
      721 tagaagcttg tcatctataa atactctgat tagttttctg tccttataag catcaatgtt
      781 ttccggtaat tctaccaagc ctatagctgt acctccatta ttgtcagcta taataagagc
      841 atagccttca ttaaaagaac ttaaatattg tatgtttaac gatgccggaa ttacttcagc
      901 accatattta tttagcatga ctatgaattc ttttgggctt ggaccatagg ttgacaatgc
      961 tcttatgaga ccatctctca tgtattctcc tgcgtatcta ccatattgcc aaagaggagt
     1021 caacctttca ccatctctat taattagaaa acttgagccg tagccatttg gataattttg
     1081 attttctata acaacataga cagcttctcc tataaacgga tttgcttctt tgtattttac
     1141 aggtaataca tagtctccat ttttatttat atacccgtat tttcctgaac tgtcccaggc
     1201 aactgcaaga cctccataga aactgttgtc aacatatttg tcggtttcag gcattagcat
     1261 aaaatcaatt ataatgtttc cttctttgtc tatgtaacct gccataccgt ttttgaccac
     1321 ataggcaact tcttctctaa atggcttagc ttcatcatat agcataggta taacaatatc
     1381 gccatcttta tttatgtacc cccaaagctc tgtctcaata tcttgcacag cagcaagacc
     1441 ttctgagaaa tttcctgcat ctttatatat ttgaggaatt actatttctc ccgttttatt
     1501 aataaatcct ttattttctt cataaccgcc aatagtcgta aacccttcat gaaacatatc
     1561 tcccatatta ctatgcatac caagaggtat tacttcattt ccttcactgt ctatgaaacc
     1621 ttccatttga cctggaggtg ccataagtct ccccggaaca tcaggttcat ctcgtggtat
     1681 gtatttatat acttttgcca taccatcaga aaattcatgt attccattgt aatcaccaac
     1741 agctattatc ttaccggttg tgtcaaaaca tatagtttgt cctgataatc ttagagtaaa
     1801 gatcccttca ctgaatatat ccatctgtga ataagactgt atatgtgagt ctccaatatc
     1861 aagccaagtg atatcaagtc ccgggaattc aagtcccctg cttgcttttt cttggtcttc
     1921 cgcaacaatt gactgccctt ttacatattc ggctccatat acatcttctg taacaaaagg
     1981 tgcaactgtc attagcagag ttaaaattag tatgataaaa atcctggttt tcattgtatt
     2041 tcctcctata gtattactct tcaaatatta ttattcttta ttcattgaaa attttaaact
     2101 gaaataattt tctttcgtat tttctgacgg cactaatata tttgttatct tattgtagcc
     2161 tgaatgctct atatcgcaat taaatgctat tccggcttca acaaattggt agtcatttag
     2221 aatattattc tctatacttc ttgctattcg tttcgggatt tttgaaaata gccccatttc
     2281 attctgtgcc cctgctaaag caagggatgc caaaaacata tgcttatcat atgaactaat
     2341 catataatca tggtttttca tttcaatgtc aaatgataca cctgtcacat atccgttttc
     2401 tatcgtaaat ttatattccg gcttttcagt taaattgata taaattacag tcccgtcagt
     2461 ttccttctct gcccatttcc cgtcttcatc caagtattca ttaccaaaat caattttgta
     2521 cagctttgca taatagttgt aattctctgc aaattctgct atatttatat ctcctttgtt
     2581 gggaggaagt tgctgtgcag aggatatcat aaaaaaagca gcaaatgttg ccaaagttgc
     2641 accgacataa atcac
//
LOCUS       DAOSOW010000270         2652 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_38607_length_2652_cov_3.627262, whole genome shotgun sequence.
ACCESSION   DAOSOW010000270 DAOSOW010000000
VERSION     DAOSOW010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38607_length_2652_cov_3.627262"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..797)
                     /locus_tag="PLD67_07170"
     CDS             complement(72..797)
                     /locus_tag="PLD67_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT22338.1"
                     /translation="MIIRYIYHSCFLIETMNSFLLFDYFNHKNDKTDFDFSKLLKDVF
                     SSPKSLYIFSSHSHQDHFNYNVLSWINKKPSTYYILSSDIKIYNKIDNLFIVRPRDEL
                     TINEIKLNIFGSTDEGVSFMVNIDGLNLFHAGDLNWWKWYDDTPEEEKTMEDNFKSIM
                     ADIVKHGVKIDIAFFPVDRRLENNYLCGAQYFIDKLNPKLFIPMHFWDDYKVTSDFKN
                     AIAGHGTNIIEIKHPNEILYANI"
     gene            988..2640
                     /locus_tag="PLD67_07175"
     CDS             988..2640
                     /locus_tag="PLD67_07175"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOT22339.1"
                     /translation="MKIENIVLNNNIQRALDDMGFEESTQIQQEAIPLILEGKDVVGQ
                     SNTGTGKTAAYAIPILEKIDKELRVPQALILLPTRELAVQVANEVRKIGKYMEGIKAV
                     TVYGGADIREQINKLKGGAQIIVGTPGRIIDLIERHVIKLSELRIAVLDEADEMLKMG
                     FREDIELILSKIDHPVQSLLFSATIPDDIRKIIKRFLNSPVYVKVLREGITAKEVKQS
                     YFLVKHSDKVEALSRLIDTYTPKLTLVFCNTKKAVDELYNKLIDKGYNCDKIHGDIKQ
                     SQRLDTLNKFNNGLIDILIATDVAARGLDIKEVDLVINYEVPSKEDYYVHRIGRTGRA
                     GREGASFTLVSAKELRKIEDIEKYTKKSIRKRAIPTVDRVNEVKQDKFIRDIVNVIES
                     GDLGENRELAEKLMDKGYDAETVIAAMIKKLVKFDLSEERDLNEVIPERKKRGGKLEN
                     TERFHINLGKKQGIRPGDILGAVAGECDIPGYDIGEIDVLDNFSFFAASKEHKKRILD
                     RMNRSQIKGMDVVVELSKEKKSRFGRDKSSDDNSSRKRKKRK"
BASE COUNT         1024 a          371 c          499 g          758 t
ORIGIN      
        1 atgaataact ttcattagaa ggcaacgaca agaacgtgct atccatagaa atgacaatga
       61 agaagataat actatatatt tgcatacaat atttcattgg ggtgcttgat ttctattata
      121 tttgtgccat gcccggcaat tgcattttta aagtctgatg taactttgta atcatcccaa
      181 aaatgcattg gaataaacag cttagggttt aatttatcaa taaagtattg agcgccgcac
      241 aagtaattat tttcaagcct tctgtcaaca ggaaagaatg caatatcaat tttcacgccg
      301 tgctttacaa tatctgccat aatgcttttg aaattatctt ccatagtttt ttcttcttca
      361 ggcgtatcat cataccattt ccaccagttt aaatcaccgg catgaaataa gtttaaaccg
      421 tctatattga ccataaaact aactccttca tcagtagaac caaaaatatt cagttttatt
      481 tcattgattg taagttcgtc tcttggtctt actataaata gattatcaat tttattataa
      541 atttttatat cagaggataa tatgtaatat gtactcggtt ttttatttat ccaggataaa
      601 acattataat taaaatggtc ttgatgtgaa tgagagctga atatataaag agatttaggg
      661 cttgagaata catctttgag caatttagaa aaatcaaaat cagttttatc atttttgtga
      721 ttgaaataat caaaaagcaa aaatgaattc attgtttcta tcagaaaaca gctatggtaa
      781 atatatcgaa ttatcataat acctccacaa atatatatga gtttcttaat tataatataa
      841 tcctaaatta tgagcaatac tataattaaa tataacttaa gacagtatgc tcataaattt
      901 ttgtaattac aaaataaatg tttgtaatta ttaaatccgc attatataat gagaagttga
      961 aaaaaatgga aagaggtaaa aaaatcaatg aaaattgaaa atatagtttt aaataataac
     1021 attcaaagag ctttggatga tatgggcttt gaagaaagca cccaaatcca gcaagaggca
     1081 attcctttaa tattggaagg gaaagatgtt gtaggacagt caaatacggg aaccggaaaa
     1141 acagcagctt atgcaattcc tattcttgaa aaaatagaca aagaattgag agtgccccag
     1201 gcattaatcc ttctgccaac gagagagctg gctgtccagg ttgccaatga agtaagaaaa
     1261 ataggcaaat atatggaagg tatcaaagct gtaacagtgt atggaggagc tgatatcaga
     1321 gaacagataa ataaacttaa gggaggagct caaataattg ttggaacccc cggcaggatt
     1381 atagatttga ttgaaagaca tgtaataaaa cttagcgagc taagaattgc tgttttggac
     1441 gaagctgatg aaatgcttaa aatgggattt agagaggata tagagcttat tttaagcaaa
     1501 attgaccatc ccgttcaatc cttattgttt tccgcaacaa ttcccgatga tatcagaaaa
     1561 ataattaaga gatttcttaa ttcccctgtt tacgtgaagg ttttaagaga aggaataaca
     1621 gccaaggaag ttaaacaaag ttattttttg gtgaagcatt ccgataaggt tgaagcactt
     1681 tccaggttaa ttgacaccta tacaccaaaa cttacattgg tattttgcaa tacaaagaag
     1741 gcagtggatg agttatataa caagcttatc gataaagggt ataactgcga taaaattcac
     1801 ggagatataa aacaatcaca aagattagat actttaaaca agtttaacaa tgggcttatt
     1861 gatatactta tagctacaga cgtggctgca agaggccttg acataaaaga agtagatctt
     1921 gtaataaact acgaagttcc ttcaaaagaa gattattatg ttcaccgtat aggcaggacg
     1981 gggagggccg gaagagaagg agcatcattt accttggtaa gtgcaaaaga attaagaaaa
     2041 atagaggata ttgaaaaata taccaaaaaa tcaatccgaa aaagagcaat acccacagtg
     2101 gacagggtaa atgaagttaa acaggataaa tttataagag atatagtaaa tgtaatagaa
     2161 agcggagatt taggggaaaa cagagaactg gcagaaaaac ttatggataa gggctatgat
     2221 gccgagactg taattgcagc tatgattaaa aagcttgtta aatttgattt atcggaagag
     2281 agagatttaa atgaagttat tcccgaaaga aaaaagagag gcggaaagct tgaaaataca
     2341 gaaagattcc atattaatct cgggaaaaag caagggattc gaccgggtga catattagga
     2401 gcagtagcag gagagtgcga tattcccggc tatgatatcg gagaaataga tgtactggat
     2461 aatttttcat tctttgctgc ttcaaaggag cataaaaaaa gaattcttga tagaatgaac
     2521 cgctcgcaaa taaaaggaat ggatgttgtt gtagaattat ctaaagaaaa aaaatcacga
     2581 tttggaagag ataaatcatc agatgacaac tcatcccgaa aaagaaagaa acgaaaatga
     2641 ttaactaacc ca
//
LOCUS       DAOSOW010000271         2637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_38884_length_2637_cov_3.625097, whole genome shotgun sequence.
ACCESSION   DAOSOW010000271 DAOSOW010000000
VERSION     DAOSOW010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38884_length_2637_cov_3.625097"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="PLD67_07180"
     CDS             complement(<1..353)
                     /locus_tag="PLD67_07180"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22340.1"
                     /translation="MADILILTASFGMGHKSVSNALKEQIEAEYKHVKVSIEDVLEIV
                     NPKVKKFSSKIYSELTESYPSIYNKLYDVKAKNKNNIIDNVLSSLYYKRFYEYMEIEK
                     PKVIISTFPLCSCIVA"
     gene            complement(591..1109)
                     /locus_tag="PLD67_07185"
     CDS             complement(591..1109)
                     /locus_tag="PLD67_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HOT22341.1"
                     /translation="MKITIIGYSSSGKSTLAAKLGNIYNCPVLYLDRVHFKENWIERD
                     DETAKAMVKEFMDHNDNWVIDGNYTNLLREQRLKEADMIIFMCFSRLACLRQAINRYK
                     TYKGKTRESMADNCSEKLDIEFIKWILLDGRSKEKKEQYAETIRKYNQKIVILRNKKE
                     TASFLEKITHRK"
     gene            complement(1142..1900)
                     /locus_tag="PLD67_07190"
     CDS             complement(1142..1900)
                     /locus_tag="PLD67_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOT22342.1"
                     /translation="MDNKNYKERIESNRTFMKSAFTEKGYKSDQQKQLPQPPLAKQSV
                     NENRIALTKEFSGIIKESNILTIINERKSHRVFKNDSITLEQLSFLLWVTQGVKGIRG
                     DNYATERTVPSGGARHPFEVYIAVINVEGLEKGIYHYLSLSHELELIRLLENIENSVV
                     ESLDNQKWAGKSAAVFYYTAIPYRSEWRYTISAHKLVLLDAGHAVQNLYIGCGAIGCG
                     TCAAADYNQELADKLLQVDGEDEFVVYIAPLGLI"
     gene            2056..>2637
                     /locus_tag="PLD67_07195"
     CDS             2056..>2637
                     /locus_tag="PLD67_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6143 family protein"
                     /protein_id="HOT22343.1"
                     /translation="MNNHLLFKQNQQLAGAVNINSNLMNYTVNVPISLMKSIEGKYFV
                     GTAPNLRFGNATNAWARLYNPPNSGVNLFVNTWTVSDIFTTPYSVQIWFNSTPPGFIQ
                     FSQSVTPSNLTIIPQPQSHIQLQYAISVSGLPTGGIKAYGRYGLAGTTINSEEEGKFI
                     FPPGGSYLIFLSNPERPTLLATGNIAFGWWEEPI"
BASE COUNT          776 a          514 c          377 g          970 t
ORIGIN      
        1 gcaacaatgc atgaacacaa gggaaaagtg ctgattataa ccttcggttt ttcaatttcc
       61 atatattcat aaaatctttt ataatataag ctgctcagca cattgtctat tatattgttc
      121 ttattttttg cctttacgtc atacagcttg ttataaattg atggatagct ttctgtcaat
      181 tctgagtata tttttgaact aaatttttta accttggggt ttactatttc caatacatct
      241 tcaatactca ccttcacatg cttatattca gcttcaattt gttcttttaa ggcattggaa
      301 acacttttat gccccattcc aaaagatgcg gtcaatatca aaatgtcagc catataaatg
      361 ctcctttctt catatgtaat tgaattctaa atacataaac atataatgat atagttagta
      421 ttagatttat atattaatta taatatagaa tattattaat ttttcattaa aaaaaggtaa
      481 atttggatct acctttttat aaaaaattat cataatacat aaatttttgc ggttcgttcg
      541 gctcaaaata cacctcgaag agctttttat ccttaacaca taataatatt tcatttccta
      601 tgcgtaattt tctccaaaaa agaagcggtt tcttttttgt ttcttaatat cacaattttt
      661 tgattatatt tacggattgt ttcagcatac tgttcttttt tttcttttga tcttccatcc
      721 agtaaaatcc atttaatgaa ctcgatatcc aatttttcag aacaattatc agccatgctt
      781 tcacgagttt ttcccttata tgttttgtat ctgttaattg cctgcctcag acacgccagc
      841 cttgaaaaac acataaatat tatcatatct gcttctttta atctttgctc tctaagaaga
      901 ttagtataat tgccgtcaat gacccagttg tcgttgtggt ccataaattc cttcaccatg
      961 gccttagccg tttcatcatc ccgttctatc caattttctt taaagtgtac tctatccaaa
     1021 tataaaaccg gacaattata aatattcccc agcttagctg ccaaggtgga cttgcctgaa
     1081 ctgctgtaac caattattgt aatcttcatg aaaacacctc gtatttattg tttgatgtct
     1141 cttatataag tccaagagga gcaatatata ctacaaattc atcctcgccg tccacttgca
     1201 gtaatttatc ggctaattcc tgattataat ctgctgcggc acaagttccg catcctattg
     1261 ctccgcatcc aatatataag ttttgaactg catgaccggc atccaataat actaatttat
     1321 gcgcgctgat tgtatacctc cactcacttc tatatggtat ggcagtataa tagaatacag
     1381 ctgccgattt ccctgcccat ttttgattgt cgagactttc gactacagag ttttctatat
     1441 tttctaataa cctgattaac tctaattcat ggcttagcga cagataatga taaattcctt
     1501 tttctaaacc ttctacattt ataactgcta tgtaaacttc aaatggatgt ctggcacctc
     1561 ctgacggaac agttctttcc gtagcataat tatcgcctct tattcccttt acgccttgag
     1621 taacccataa taagaatgac agctgctcta atgtaatact atcgttttta aaaacacggt
     1681 ggctttttct ttcattgata atcgttaaaa tatttgattc tttaattatg cctgagaatt
     1741 ctttggtaag tgcaattcta ttttcattga ctgattgttt agctaaaggc ggctgaggca
     1801 gctgcttttg ttggtctgat ttatatcctt tttccgtaaa tgcacttttc atgaatgttc
     1861 tgttgctttc tattctctct ttataatttt tgttatccat aataccatta tctaatggat
     1921 tgctccattt ttcccctttc taaattattt tattattaaa gattacgttt gtaaagttca
     1981 gatagacatg gtaaattcac ctttcatata ttatatcata taataaataa ctataatatg
     2041 aaaggttgaa atctaatgaa taaccatcta ttatttaaac aaaatcaaca gcttgcagga
     2101 gctgtaaata ttaattcgaa tttaatgaat tacactgtta atgttcccat aagcttaatg
     2161 aaatctattg agggaaaata ttttgtcggg acggctccaa acctgcgatt cggtaatgct
     2221 accaatgcat gggcaaggct gtacaacccg ccgaattcgg gtgtaaattt atttgtaaat
     2281 acttggactg taagtgacat ctttacaact ccatacagtg tacaaatttg gtttaacagt
     2341 actcctcccg gatttatcca attttctcaa tctgttactc catccaattt gacaattatt
     2401 ccacagccgc agtctcatat acagctccaa tatgctattt cggtcagtgg tttgccaaca
     2461 ggcggaatca aggcttacgg cagatacggt cttgccggta ctaccataaa ttcagaggaa
     2521 gaaggaaaat ttatatttcc tcccggcggt tcatatttaa tttttttgtc aaaccctgag
     2581 agaccaaccc tgctcgctac aggcaatatt gcatttggat ggtgggaaga accaatt
//
LOCUS       DAOSOW010000272         2636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_38904_length_2636_cov_2.817513, whole genome shotgun sequence.
ACCESSION   DAOSOW010000272 DAOSOW010000000
VERSION     DAOSOW010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38904_length_2636_cov_2.817513"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLD67_07200"
     CDS             <1..531
                     /locus_tag="PLD67_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014822314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOT22344.1"
                     /translation="STEVKNGGTVDTKTLIRPKVEGEVAFVLKEDLKGPGITVEDVYK
                     ATEYICASIEIVDSRIKDWKIKLVDTIADNGSSALYILNDKKVKIDDVSLPDIRMTLY
                     KNGEVVNSGTGKDVLGNPAISVAWLANKLCEYGITLKKGEIILSGAITASPEAFKGDM
                     FECVFPELGNVKVEFK"
     gene            697..1350
                     /locus_tag="PLD67_07205"
     CDS             697..1350
                     /locus_tag="PLD67_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22345.1"
                     /translation="MKDSGCKKREYRDNPNVINNINTCTDTKNSNNLATGNSCTPEEL
                     IRNGGFEEVGIQTVFANWNDETEGLDINIRPSTSITYEGRQSANFDIGPVPGNTTDRA
                     TLTQTVSVNPGCYLTLSFAERLAEKPVDIELYFSAEVFYISGSNEVELIKIEKIAPRA
                     VTVESSAFSYHEKTSDIIVPQNVNFVIVRFTVSINTINASNNDTDLYLDGVSLRASR"
     gene            2313..>2636
                     /locus_tag="PLD67_07210"
     CDS             2313..>2636
                     /locus_tag="PLD67_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase XerC"
                     /protein_id="HOT22346.1"
                     /translation="MMYINYNDLCPSPLSDFLEYSIAIKGRADKTVSQYFLDLRTFYR
                     FLVIKFKLSPDVENFEEIDISPVTLDIIKKVKIMDLHSFIAYTDRERKNSNRTKARKM
                     SALRSY"
BASE COUNT          941 a          384 c          452 g          859 t
ORIGIN      
        1 agcacggaag taaaaaacgg cggtacagta gacacaaaaa cgttaataag gccaaaagta
       61 gagggtgaag ttgcttttgt actgaaagaa gatttaaagg gtcccggtat aactgtggaa
      121 gatgtttata aagcaactga atatatatgt gcttccattg aaatagtgga ttcgagaatt
      181 aaagattgga aaattaaact ggtagatact attgcagaca acggttcaag cgctttgtat
      241 atattaaacg ataaaaaagt aaaaatagac gatgtgtcat tacctgatat aaggatgaca
      301 ctgtataaaa acggggaagt ggtaaacagc ggaacaggta aggatgtatt agggaatccg
      361 gcaataagtg ttgcatggct tgcaaataaa ctgtgtgaat acggaattac tcttaaaaag
      421 ggcgaaataa ttttatcggg agcaattact gcatcaccgg aagcatttaa aggtgatatg
      481 tttgaatgtg tttttcctga actggggaat gtcaaggtag aattcaaata ataacagata
      541 ataacaaaac atcggggctg aaaagctccg atgttttttt gattctccgt catttaatac
      601 aatttatgcc acatttattt atgtaacaat tataaaggtt gtacatatta tatttatgag
      661 cttaagcttg ttttatatat tggaggcgtt aacattatga aagattcagg ctgtaagaaa
      721 agggaatata gagataatcc taatgtaata aataatataa atacttgcac agatacgaaa
      781 aacagcaata atttagcaac aggaaattca tgtactcctg aagagcttat aagaaatggt
      841 ggctttgaag aagtaggaat tcaaacagtt tttgctaatt ggaatgacga aacggaaggt
      901 cttgatataa atatacgacc aagtacttct ataacatatg aaggcagaca aagcgccaat
      961 tttgatattg gtccggtacc cgggaataca acagacagag caacattaac acaaacagtt
     1021 agtgttaatc ccggatgtta tttgacgcta agctttgcag aaagattagc agaaaaaccg
     1081 gtagatatag aattatattt tagtgctgaa gttttttata tttcaggttc taatgaagtt
     1141 gaattaatta aaatagaaaa aatagctcca agagctgtta cagtagaatc atcagcattt
     1201 tcttatcatg aaaaaacgtc agacataatt gtacctcaaa atgtaaattt cgtaatagta
     1261 agattcactg tcagcattaa tactatcaac gcaagcaata atgatacaga cttatatttg
     1321 gacggagtat cattgagagc ttctcgttga ccgggatatc aagggaatag cagtaataat
     1381 tacaaaattg ttaatttata taaatattat tattttttat tatgtcgcta aatgctaatt
     1441 tagcgacata atatccctga aataagcact tttccgaaag accccttctt tttggaattt
     1501 caacgttctt ctcgattttt ttatcgattt gattattttt cttttataat ttcaattcac
     1561 aaatttataa ttcttgtaat attttttaat aatttacctt tgcggtttgg atattttttt
     1621 ctttacagtt ctgtcctgat tgttgtagct cgaaaatctc accggatctt tctcttatgt
     1681 attagatact acgcttattc ttataatgac aatgttaata gttactacgc tatgctcaag
     1741 atgaaaaaat aaaaatattc gttcactctt gcacactata gacaaaaaac gaccaagtca
     1801 agtaaccggc tgaaatttca aagatatgcc caaaaaacga ggctttaaaa tgcgatttaa
     1861 cgcacgaaac ttctttcctt gaccatttat accctttaaa tatagctcgt ttttaattaa
     1921 caggcaaaat ttgatttcta tcatctataa gacagatata ttcctcatac ctctaagact
     1981 tgtccgaatt ggggactttc ctctaatgct catcagagaa tactaagttt aatgagatgc
     2041 tcaaaatttc aattaatgtg tggttatttt ttattaaaag cagatatacg ggtcgaatat
     2101 atgaataaat actggttata tgtcgaagta tcgaaaaaag ttaattgcaa ttcacacaat
     2161 acttgtaaat ttgagcactt gcatttaaga aaagtatttt tgtgttattt tattaatcaa
     2221 aatgatatag gttttactaa aaaagcttgt ctttttccta attcttctat ataattaagt
     2281 aagaattttg cgacattttt gtaaaggaga taatgatgta cataaattat aatgacctat
     2341 gcccttcccc acttagtgat tttttagaat actcaattgc tattaaaggc agagcggata
     2401 aaactgttag tcagtacttt cttgaccttc gtacctttta tagatttcta gtaatcaaat
     2461 tcaaattaag tcctgatgtt gaaaattttg aagaaatcga tatctcccct gttaccttgg
     2521 atattataaa aaaagtaaaa attatggacc ttcattcttt tattgcctat acggacaggg
     2581 aaagaaaaaa ttcaaaccgt acaaaagctc gaaaaatgtc agcacttagg tcatac
//
LOCUS       DAOSOW010000273         2625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_39099_length_2625_cov_4.023346, whole genome shotgun sequence.
ACCESSION   DAOSOW010000273 DAOSOW010000000
VERSION     DAOSOW010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39099_length_2625_cov_4.023346"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="PLD67_07215"
     CDS             <1..251
                     /locus_tag="PLD67_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HOT22347.1"
                     /translation="TIENYKIKTSNGTFAEFKEIQSKKKLDKDVNMQIILLENRLSDI
                     VGRLSMQLKKEELEALDKEYYEVLEKLNKLKTLRTTPV"
     gene            complement(277..756)
                     /gene="rlmH"
                     /locus_tag="PLD67_07220"
     CDS             complement(277..756)
                     /gene="rlmH"
                     /locus_tag="PLD67_07220"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOT22348.1"
                     /translation="MKITIISVGKIKEKFFTEAIKEYIKRLSKYCKFIEEIVPDERAD
                     ESFSPAEIEQVKIKEGQKILNKIPKNSYVIVLDINGAQLSSEAFAEKLNTFGIEGISD
                     ITFIIGGSNGLWKEVIKSADFNLSFSKMTFPHQLFKVILLEQIYRAFKINSGETYHK"
     gene            1018..1380
                     /locus_tag="PLD67_07225"
     CDS             1018..1380
                     /locus_tag="PLD67_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22349.1"
                     /translation="MIKIKKIICLVICISMLGTQAFGFSQAYNEFDIKKSIENTYGIN
                     IIIPEGVDYSEFIDCMSILERSIKKFPDNIIKEITDYYLSKGIATNVILDKTEIIKEL
                     FTLSIIDESSVNIYIKTL"
     gene            1459..2379
                     /locus_tag="PLD67_07230"
     CDS             1459..2379
                     /locus_tag="PLD67_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT22350.1"
                     /translation="MLETYDLDNLKDEFDKLNEGFEYGTWSEEYSKVFVNRHSAISFE
                     NDIAHLIWYAEANPFILRNINGGDYAIIHEKLRLIAEEFSKIFNSITENTKLWLDAIP
                     QTPQDWAKDTITEMKNQSLIPDEFNGLYEAYIKRGDFYKLVLNMLHIKLGENELNKYF
                     NLTDYEEHVALDPLRGEVYVTDGLNYTSYYNYLANSSNAFYEAYQMGILNTDSFYEPD
                     EYMTRLEIAKLLAYIANELGMNISEFQTVSFNDIEQVAENEKPYIYIAADKGFIKGDG
                     LNFKPFNYCTYQEAYLILNRFYNSLYDYIQ"
     gene            2400..>2625
                     /locus_tag="PLD67_07235"
     CDS             2400..>2625
                     /locus_tag="PLD67_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22351.1"
                     /translation="MFDLKSIVIGLVLMIKKYSCLSLTIYEEANEEDYKNGIIALPII
                     GLAIGLVAFIIVSFRYIYDGFFISSAVLLYY"
BASE COUNT          947 a          363 c          434 g          881 t
ORIGIN      
        1 tgactattga aaattataaa ataaaaacat ctaacggtac ttttgcagaa ttcaaagaaa
       61 tacaaagtaa gaaaaaacta gacaaagatg ttaatatgca gataattttg ttagaaaaca
      121 ggctttccga tatcgtagga agactgtcta tgcaattaaa aaaggaagag ctagaagctt
      181 tggataaaga atattatgag gtcttagaaa agttaaataa actaaagacg ttaagaacaa
      241 ctcccgtata ggataatccc ggagttgtcg gaattattac ttgtggtagg tttcgcctga
      301 atttattttg aaagctctgt aaatttgctc taataaaata actttaaaca gctggtgggg
      361 gaaggtcatt ttggaaaatg aaagattaaa gtcggcactc ttgattactt ctttccaaag
      421 tccgttgctc ccgcctataa taaaagttat atccgaaata ccttcgattc cgaaggtatt
      481 taatttttcg gcaaaggctt cggagctaag ctgagcaccg tttatatcca aaacaattac
      541 ataagaattt ttgggaattt tattaagtat tttttgccct tcttttatct taacctgctc
      601 tatttctgca ggtgaaaaac tttcatccgc cctttcatcg ggcacaatct cttcaataaa
      661 cttgcaatat ttcgaaagac gtttaatata ttccttaatt gcttcagtaa aaaacttttc
      721 ttttattttg cctacggata ttattgttat tttcatttca atcctctgtt aatattaatc
      781 atatagtatt ttttacaata tattattata ccattatatt attgaattta ccagactaag
      841 agtaagttaa atcctacaga atgaaaatta acgcttattc atagcaaaac tccggatggc
      901 actcttagcg taaacgtttg caaattttga ttgcattttg ttttaagcta tgctataatt
      961 tttatacttt taacaatatt tcatccaatc aaataattta tcggcgaggt aaataagatg
     1021 attaaaataa aaaaaataat atgtttggta atatgtatta gtatgttagg aactcaagct
     1081 tttggtttca gccaagctta taatgaattt gatattaaaa aaagtattga aaatacttac
     1141 ggaataaata ttataatacc ggaaggtgtt gattacagcg aatttattga ttgcatgtca
     1201 atattggaaa ggagtattaa gaaatttccc gacaatatta ttaaagaaat taccgactat
     1261 tatttaagca agggaattgc tacgaatgta atattagata aaacagaaat aataaaagaa
     1321 ttgtttacat tatcaataat agatgaaagc tcagtcaata tttacataaa gaccttgtaa
     1381 ggcagtctgt attctgaatc tcattatgca tctgaacaag ttataacaca tgtttttagt
     1441 catttcataa gtgattatat gcttgaaaca tatgatcttg ataatttaaa agatgaattt
     1501 gacaagttaa atgagggatt tgaatacggt acctggagcg aggagtattc aaaagttttc
     1561 gtcaaccggc attccgcaat cagttttgag aatgatattg cacacttaat atggtatgcg
     1621 gaagcaaatc cgttcatact tcgtaatata aacggcgggg attatgcaat aatacatgaa
     1681 aaactcagat tgattgccga agaattcagt aaaatcttta attcaattac agaaaataca
     1741 aaactctggc ttgatgcaat acctcaaact cctcaggatt gggctaagga cacaattacg
     1801 gaaatgaaaa atcagtctct tattccggat gaattcaacg ggctgtatga agcttatatt
     1861 aaaagaggag atttctataa actggtttta aacatgcttc acattaaatt aggagaaaat
     1921 gaattaaaca aatactttaa tttaacggat tatgaagagc atgtggcatt agaccctctt
     1981 aggggagaag tttatgtaac cgatgggttg aattatactt cttattataa ttatttggca
     2041 aactcaagca atgcatttta tgaagcatat cagatgggaa ttttaaacac agattccttt
     2101 tatgagccgg acgaatatat gacaagactt gaaatagcaa aactattagc atatatagcc
     2161 aatgaattgg gtatgaatat ttcagaattt cagaccgtat cttttaatga tatagagcag
     2221 gttgcagaaa atgaaaaacc ctatatatat attgcagctg ataaagggtt tataaaagga
     2281 gacggattaa acttcaaacc gtttaattat tgtacatatc aggaagcgta tttaatccta
     2341 aatagatttt ataacagcct atacgattat attcaataat ataaaattga ggtagtatta
     2401 tgtttgactt aaaaagtatt gttataggcc ttgtattaat gataaaaaaa tattcctgtt
     2461 taagtcttac catttatgaa gaagcaaatg aggaggatta taaaaacggt ataattgcac
     2521 ttcccataat aggacttgcc attggattag tagcatttat tattgtttcc tttagatata
     2581 tatatgacgg tttttttatc agttctgcag ttcttttata ttact
//
LOCUS       DAOSOW010000274         2621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_39182_length_2621_cov_2.744739, whole genome shotgun sequence.
ACCESSION   DAOSOW010000274 DAOSOW010000000
VERSION     DAOSOW010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39182_length_2621_cov_2.744739"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..1401)
                     /locus_tag="PLD67_07240"
     CDS             complement(97..1401)
                     /locus_tag="PLD67_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HOT22352.1"
                     /translation="MKQFFKLEENNTNVKTEVLAGITTFMTIAYILIVNPIILSETGM
                     DFGGIFTATALSSLIATLMMALYANYPFALAPGMGLNAFFAFTVVLGPMGKSWEFALT
                     AVLIEGIIFILLSFIKAREAIFESIPLNLKNAVSVGIGIFISFIGLSGAGIVKAGNGT
                     LVALGELTSGAPLLAIIGIIITGLLLIKNVKGALLIGIVITTIIGIPMGVTILPENFS
                     TIVSMPPSLKDVAFKFVGFDQIFSFEMLVVVFTFLFVDIFDTVGTLAGVATKANMLDE
                     EGKLPRVGAALMSDAVGTVVGACLGTSTVTTYVESASGVAEGGRTGMTSLVTAAMFGV
                     ALFFAPLFTLVPSAATAPALVIVGLFMMSPITKINFNDYTEAIPAFLTIVMMPFAYSI
                     AEGIVFGMVSYVILKAISGKFKDISLVMWILSVLFVLRFIFI"
     regulatory      complement(1569..1668)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(1739..>2621)
                     /locus_tag="PLD67_07245"
     CDS             complement(1739..>2621)
                     /locus_tag="PLD67_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HOT22353.1"
                     /translation="KYIIDVKIRGNNVIEKIFAEHMPSTFMSVLTDGCKESGRDYNAG
                     GSKYNTRYIQVVGIATITDCLSSIKYNVYDNKRFTMGELLKALEANFEGYAHIKNLVS
                     NHTPKYGNDDDYADDIMIDIFTDVRDLIKGRKTVCGGTYQIDMLPTTCHVYFGSVMGA
                     SPNGRLAKKPVSDGISPEKGADVNGPTAVIKSCSKMDHTSTGGTLLNQKFSPSSIAGE
                     EGIENVSSLIRSYFELIGHHIQFNVIDKKTLLDAQKHPEEYKDLIVRVAGYSDHFNNL
                     EKSLQDEIIERTEQSFN"
BASE COUNT          850 a          526 c          400 g          845 t
ORIGIN      
        1 taaaatgcaa cactttttct aaaaattttt ttaatctatt taaaaataaa aaaacctgcc
       61 gtttatctcc aacggcaggc tgttttatct tttactttaa ataaatataa atcttaaaac
      121 aaatagtacc gataaaatcc acataaccaa agatatatct ttgaatttgc cggatatagc
      181 tttcaaaatc acatatgata ccattccaaa tacaattcct tccgcaatac tatatgcaaa
      241 aggcatcata acaattgtta agaaagccgg tattgcttct gtataatcgt tgaaatttat
      301 tttggttata gggctcatca taaacaaacc tacaataacc aatgcaggtg cagttgcagc
      361 tgatggaacc aatgtgaaca atggagcgaa aaacagtgca actccaaaca ttgctgcagt
      421 aacaagcgat gtcattcctg ttcttccgcc ttcagcaacg cctgaagcac tttcaacata
      481 tgttgtaaca gtacttgttc ctaaacatgc accaacaacc gtaccaacag catcagacat
      541 tagagcagct cctactctcg gcaatttccc ttcttcatcc agcatatttg cctttgtggc
      601 aacgcctgcc aatgttccga cagtatcaaa aatgtctaca aagaggaatg tgaatactac
      661 caccagcatt tcaaaagaaa atatttggtc aaatcctaca aatttaaatg caacgtcctt
      721 aagtgaagga ggcatgctta caattgtact gaaattctcc ggcagtattg taactcccat
      781 cggaattcca ataattgttg ttataactat tcctattaac aatgctccct taacgttttt
      841 tatcagcaat aatcctgtaa taataattcc tataatagcc aataacggag cacctgatgt
      901 caattctccc agagcaacaa gcgtaccgtt gcctgctttt actattcctg caccggataa
      961 acctataaaa gatataaata tacctattcc aacggaaaca gcattcttta aatttaaagg
     1021 tatagattca aatattgctt ctctggcttt tataaaggat agtaaaataa atataattcc
     1081 ctcaattaat acagcagtta gagcaaattc ccatgacttg cccattggtc ccaatacaac
     1141 agtgaatgca aagaatgcat taagtcccat accgggggct aaggcaaacg gatagttggc
     1201 atagagggcc atcataagtg ttgcaataag tgatgataag gccgtagcag taaatatacc
     1261 gccaaaatcc attccggttt ctgatagaat gataggatta acaattaaaa tataggctat
     1321 tgtcataaaa gtagtaatgc ctgctaatac ttctgttttt acattagtat tattttcctc
     1381 aagcttaaaa aattgtttca aagtattatt cctcctgatt ttttaatatt ttatcatatg
     1441 aattaaccac aaaaaataaa taaagcctgt cgaggtagga agtgaaacct acccgactgg
     1501 gcttttatcc cttcggtgta atacttaaag tatctgtacc gctcggtcca gcaagcaatc
     1561 ttcggaaccc tagatacact ttcactcata gccaaatgat taggtcattt tgtagaaaca
     1621 tctcaaccat ataatgagat atatatgagc tatatttatt ttcatattga taataccaat
     1681 atgttttaaa tatgtcaata ggaaaatgat gtatacttaa aacgcttgaa gtatgagctt
     1741 aattgaaaga ctgctctgtt ctttcaatta tttcatcctg caaagatttt tcaagattgt
     1801 taaagtggtc cgagtaaccg gctaccctaa ctattaaatc cttgtattcc tccggatgtt
     1861 tttgtgcatc taagagagtc tttttatcaa ttacgttaaa ttgaatgtga tgacctatca
     1921 attcaaaata tgaccttatt agtgaagata cattttctat tccttcctcg cctgctatgg
     1981 atgaaggtga aaatttttga ttcagcaagg ttcctcccgt agatgtatgg tccattttag
     2041 agcatgattt gattaccgca gtagggccgt tcacatctgc acctttttcg ggggatatac
     2101 cgtctgacac aggttttttt gctaaccttc cgtttggaga agcacccatt accgagccga
     2161 aatatacgtg gcaagtagta ggaagcatat caatttgata tgtacccccg cacacagtct
     2221 ttcttccttt tatcaaatct cttacgtcag taaatatatc tatcattata tcatcagcat
     2281 aatcatcatc attgccgtat ttgggcgtgt gatttgatac caaatttttt atatgagcat
     2341 atccttcaaa atttgcttcc aatgctttta acaattcacc catcgtaaat cttttattat
     2401 catatacatt gtatttaatt gaagataaac aatcggtaat agtagctatt ccaacaactt
     2461 gaatatatct ggtattgtac tttgagcctc ctgcattgta gtccctgccg ctttccttgc
     2521 atccgtcggt cagcactgac ataaatgttg aaggcatatg ctctgcaaag attttttcaa
     2581 ttacattatt acctctaatt ttaacatcaa taatatattt a
//
LOCUS       DAOSOW010000275         2613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_39329_length_2613_cov_4.055903, whole genome shotgun sequence.
ACCESSION   DAOSOW010000275 DAOSOW010000000
VERSION     DAOSOW010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39329_length_2613_cov_4.055903"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="PLD67_07250"
     CDS             <1..484
                     /locus_tag="PLD67_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HOT22354.1"
                     /translation="RKEMALKTNKANKKGALSDALKGADIFIGVSAPNIVTKEMIESM
                     NENSIAFAMANPIPEILPEVALAGKVKVMGTGRSDFPNQINNVLAFPGIFRGALDVRA
                     KEINEEMKIAAAHAIAELVTEEELNEEYIIPKAFDKRVAKAVARAVSEAAIKTGVNRI
                     "
     regulatory      575..761
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            963..1904
                     /locus_tag="PLD67_07255"
     CDS             963..1904
                     /locus_tag="PLD67_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT22355.1"
                     /translation="MKRNSILVLTLICIMALLTACAENTGVKSPQPEDINITLPTKDR
                     AGNEISIPNEINRIISISPANTEILIELGFGDKIIAVDEYSKDIEGLPNNIKLFDMMA
                     PDAEQMVALEPDIIYTTGMVMAEGNDPYKPIRDMGVCVAYIPASLSIEGIYEDILFFA
                     KSLGVEKKGVDLVNSMKFKIKEIEEIGKSIENKKTVYFEIAAAPTLYSFGKGVFLNEM
                     IEIIGAENVLSDHDWWISVSEETVIASDPDVIITNVDYIENPAEEIKSRSGWENMKAV
                     KNNDVYYVDNYATSHCNHNIITGLEQMARAIYPDIYQ"
     gene            1901..>2613
                     /locus_tag="PLD67_07260"
     CDS             1901..>2613
                     /locus_tag="PLD67_07260"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT22356.1"
                     /translation="MKIKFKIILSIIFSIIVVIMGVGLGSVNVPPSHIISVILNKIFS
                     APLPSDIEGTTAISIIWKLRLPRTLLAFLVGGAVAVSGSVMQSVLKNPLASSYTLGVS
                     SGASLGAALVIVTGFNIPFLGMFTLPVTGLVFGMATVFLAVKFAAMMDKNLETNTIIL
                     VGMVFSLFVNAILTVITAMARDHLAQLTYWQMGSFSLKDWSSVIILFPIVLIGIAVLQ
                     NYSKEMDIMTFGEEQAFTMG"
BASE COUNT          911 a          417 c          535 g          750 t
ORIGIN      
        1 tagaaaagag atggccttaa agacaaataa ggcaaacaaa aaaggagctc tttcggatgc
       61 tttaaaagga gcagatatat ttataggtgt ttctgcacca aatatagtta caaaagaaat
      121 gattgaaagc atgaatgaaa attcaattgc ttttgcaatg gcaaatccga tacctgaaat
      181 attgcctgaa gttgcattgg caggcaaagt caaagtaatg ggaacaggaa gatctgattt
      241 ccccaatcaa attaataatg tattagcatt ccccggtatc ttcagaggtg ctcttgatgt
      301 aagggctaaa gaaattaatg aagaaatgaa aatagcagca gcacatgcaa ttgcagaatt
      361 ggtaaccgaa gaggaattaa atgaagaata tataattcca aaagctttcg ataaaagggt
      421 agccaaagca gtagctaggg ctgtttctga agctgcaata aaaaccggcg taaatagaat
      481 ttaaaagcta agtagtttta gtataagtat cgatataatt ttgcatattt tcttaattga
      541 caatactaat ttaatgtaat atacttatct taattaaaca tctttcgata ttggtgcgct
      601 taaatgcgat aatagggaat taggttaaaa tcctaaacag tcctgctgcc gtgatgagag
      661 agcggttttt atgcttatgc aaccactgga tatctccggg aaggttaaaa accagcaatg
      721 acctctgagt cggaaaacct gccagtatta tttgcgtaga tgccgcaaat gctatgcttt
      781 acagagtata aaagcgatag cgcaaaaatg gtttatccaa aatatttgtg gaatttacgt
      841 ttcgggaacg agacgttttt ttatgcccta agccaagact tcgaccggaa ggagaaagtc
      901 ttgatggtag gtcaaacgca atgcctctct tagatgttta aaaaacaaaa atggaggatg
      961 aaatgaaaag aaacagtatt ttagttttaa cattaatttg tattatggcc ttgcttactg
     1021 cttgcgccga gaataccgga gtaaaatctc ctcaaccgga ggatatcaac ataacattgc
     1081 ccacaaaaga cagagcgggc aatgaaatat caataccaaa cgaaatcaac agaataatct
     1141 ccatatcacc tgcaaatacg gaaatactaa ttgaattagg ttttggcgat aaaataattg
     1201 cagtggatga gtattcaaaa gatattgaag gactgcctaa taatataaaa ttatttgata
     1261 tgatggcacc tgatgctgag cagatggttg ccttagagcc ggacataatt tatacgaccg
     1321 gaatggttat ggcagaagga aacgaccctt acaaacctat cagggatatg ggtgtttgtg
     1381 tagcatatat accggccagt ttaagcatag aaggaattta tgaagatata ttattttttg
     1441 caaaatccct cggtgttgaa aaaaaaggtg ttgaccttgt aaacagcatg aaatttaaaa
     1501 ttaaagaaat tgaagaaatc ggcaaatcta ttgaaaacaa aaagacagtg tattttgaaa
     1561 ttgcagcagc accaacactt tacagttttg gcaagggagt atttttaaat gaaatgattg
     1621 aaataatagg tgccgaaaat gttttatcgg accatgactg gtggatttca gtttcagaag
     1681 aaacagttat tgcatctgac cctgatgtta tcataaccaa tgttgattat attgaaaatc
     1741 ccgcagaaga aatcaaatcc cgctcaggat gggaaaatat gaaggctgtt aaaaataatg
     1801 atgtttatta tgtagacaat tatgcaacct cccactgcaa ccacaacatt attacaggct
     1861 tggagcaaat ggcgagggca atctatccgg acatatatca atgaaaataa aatttaaaat
     1921 aatattatca ataatattca gtattatagt agtaataatg ggagtgggat taggaagcgt
     1981 caacgttccc ccttcccata ttataagtgt aatattaaac aaaatatttt cagcaccgct
     2041 tccttccgat atagaaggta ccactgctat atcaataata tggaaattaa gactcccaag
     2101 aacactcttg gcatttttgg tggggggtgc tgtagctgta agcggctcgg taatgcagtc
     2161 ggttttgaaa aatcctcttg cttcctcata cacattaggg gtatcttcag gtgcttcatt
     2221 gggagcagct ctagtaatag taaccgggtt caacataccc ttcctgggaa tgttcacatt
     2281 gcctgttacc ggcttggtat ttggcatggc gacggtattt ttggctgtta aattcgcagc
     2341 tatgatggat aaaaacttag aaactaatac aataattttg gtaggtatgg ttttttcatt
     2401 gtttgtaaat gccatactga ctgtcataac ggcaatggca agagaccatc ttgcacagct
     2461 gacttattgg caaatgggaa gtttttcatt aaaagactgg tctagtgtaa taatactctt
     2521 tcccatcgtt ttaataggaa tagcagtatt acaaaattat tcaaaagaaa tggatataat
     2581 gacttttgga gaggagcagg cattcaccat ggg
//
LOCUS       DAOSOW010000276         2602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_39555_length_2602_cov_3.780919, whole genome shotgun sequence.
ACCESSION   DAOSOW010000276 DAOSOW010000000
VERSION     DAOSOW010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39555_length_2602_cov_3.780919"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /locus_tag="PLD67_07265"
     CDS             complement(<1..1222)
                     /locus_tag="PLD67_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22357.1"
                     /translation="MAVKDRIKYTLRLRLFLFLIVLVITILLGALIILFVSGNITAGL
                     KENENFIKSEHSLIIKNTVGFYDNLAAEAVAFSRSISMSIESSLRDNDLTVDDLNNNP
                     ELLKEILSDELHQIIFYLQKSKSSGAFIILDATSNSNLPDSEYSKAGIYLQNMEPNIV
                     SSSSPTIYVLRGMADIAYKNSLPLHPQWRMEFNVADAPYYNLPMDKANKDTSLSNLYY
                     WSKAFTFPKTNEKIMMCSVPLIDIEGNVFGVCGFDVSYMLFKLLNMPDNSRYERIFCL
                     ISPVENNILKTDGSLFSGGYSARSLINNNDLKISSGKRSLYIYENSGNNFIGYHELLK
                     LYPENSSFADNEWALALLIPQEDISSVIVSTNLKFIYISAFLMILGIIISYILSKYYI
                     LPISKGFDIIKNNPK"
     gene            complement(1228..>2602)
                     /locus_tag="PLD67_07270"
     CDS             complement(1228..>2602)
                     /locus_tag="PLD67_07270"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOT22358.1"
                     /translation="FMKKLVVCILIFIMLLTGCKSNDPLSAKNPVTINLWHNYGGLLK
                     DTMDLLIDEFNDTVGREKGIIINVTSLTGSATIAEKLKMAVNDEPGAPELPDITTLYP
                     NTAYMLAEKGLLVDIESLFSKEELSLFIPEFIEEGRLPDGKIYVLPTGKSTEVMFLNT
                     TIFNKFMKETNVSYNDLYTFEGLIDIAEKYYKWTDNQTPDIDNDGKAFICYDSVFNMA
                     QTIYKQSGDELIADGSLNLSSDAFKDVWKYCFEPAVKGYSAIYDGYGSDLIKTSRVVA
                     SIGSTAGVLFYSDIVTYEDNITEPAEFLILPYPVVDGGKKVAIQRGGGMCIIKSNEAK
                     EYAAGIFLKWFTAPEQNLKFVSSTGYVPVTQMAVEMTVVNGDDIDDKIMKLQKTTKEM
                     AEQYTFFYPPVIENFDELQENYDNNIKNYAIQCKNDYFNLLSNMNDTDAFNTATENRY
                     EEFTGQR"
BASE COUNT          809 a          495 c          401 g          897 t
ORIGIN      
        1 cttttgggtt gtttttaatt atgtcaaacc cttttgatat tggtaaaata taatatttgc
       61 tcaaaatata cgagataata attcccagta tcattagaaa ggcgcttata tatatgaatt
      121 ttaaatttgt acttactatg acactgctta tatcttcttg cggaattaac agtgccaaag
      181 cccattcatt atcggcaaaa gatgaattct cagggtagag tttcaataat tcatgataac
      241 ctataaaatt atttccacta ttttcataaa tatatagaga cctttttccg gaagaaattt
      301 tcaaatcatt gttgtttatc aaacttcttg ctgaatatcc ccctgaaaac aatgagccgt
      361 ctgtttttaa aatattattt tcaaccggtg aaatcaaaca aaaaattctt tcatatctac
      421 tgttatcagg catatttaga agcttgaaaa gcatgtagct aacatcaaaa ccgcacacac
      481 caaaaacatt tccttcaata tcaatcagag gaacagaaca catcattatt ttttcatttg
      541 tttttggaaa tgtaaatgct ttgctccaat aataaagatt tgataaagag gtatccttgt
      601 tcgccttatc catgggcaaa ttataatagg gtgcatctgc tacattgaat tccattctcc
      661 attgagggtg aagaggcagg gaattcttat aagcaatatc agccataccc ctcaatacat
      721 aaattgtagg gcttgacgaa ctgactatat tcggctccat attttgtaaa tatattccgg
      781 cctttgaata ttccgaatcg ggtagattgc tgtttgatgt ggcatccaaa attataaaag
      841 cgccgctgct tttggatttt tgcaaataaa agattatctg atgaagctca tcagataaaa
      901 tttccttcaa aagctccgga ttgttattaa ggtcatcaac agtcaggtca ttgtccctca
      961 aactgctctc tatactcatg gatatagagc gtgaaaaggc tacggcttca gcggcaagat
     1021 tgtcataaaa cccgacagta ttttttataa ttagtgaatg ttcacttttt ataaagtttt
     1081 cattttcttt taatcctgcg gtaatgttgc ctgatacaaa cagtattatc aaagcgccta
     1141 acaatatagt tattacaaga actattaaaa acaaaaacaa tctaaggcgc aaggtgtatt
     1201 taattctatc ttttacagcc aaacttctca cctctgcccg gtaaattctt catatctgtt
     1261 ctcagtagcc gtattaaatg catcagtatc attcatattg ctcaataaat tgaaataatc
     1321 atttttacat tgtattgcat agttctttat attattatcg taattttctt gcagttcatc
     1381 aaaattttct attaccggag gataaaagaa agtatattgt tcagccattt cctttgtagt
     1441 tttttgaagt ttcataattt tgtcgtctat gtcatctcca ttaacaacag tcatttcaac
     1501 cgccatttgc gttacgggaa catatcccgt agatgataca aattttagat tttgctcggg
     1561 agcagtgaac catttaagaa aaattcctgc cgcatattct ttcgcttcat tggattttat
     1621 aatgcacata ccaccgcctc gttgaattgc aacctttttc ccgccgtcta caaccggata
     1681 gggcaaaatc aaaaactctg ccggctcagt tatattgtct tcatatgtaa ctatatcaga
     1741 gtaaaaaagc acaccggcag tagatccaat gcttgcaaca actctgctgg tcttaataag
     1801 gtctgagcca tatccgtcat atatggccga ataacctttc acggcaggct caaaacagta
     1861 tttccaaaca tctttaaatg catcggaaga taaattcaag cttccgtctg caatcaactc
     1921 atcgccggat tgcttgtata ttgtttgagc catgttaaaa acagagtcat aacatatgaa
     1981 ggctttgccg tcattatcaa tatcaggtgt ttggttatct gtccatttat aatatttttc
     2041 agctatatct ataagtcctt cgaatgtata caagtcatta tagctcacat ttgtttcttt
     2101 cataaattta ttgaatattg ttgtgtttaa aaacataact tccgtagatt tacccgtagg
     2161 cagcacataa attttcccgt cgggaagcct tccttcttct ataaattcag gaataaataa
     2221 tgacaattct tctttggaaa aaagcgattc aatatcaacc aacaatcctt tttcggccaa
     2281 catatatgca gtattgggat ataaggttgt aatatcaggc agttcaggtg caccgggttc
     2341 gtcgtttact gccattttta atttttccgc tattgttgcc gagcctgtga gtgaagttac
     2401 atttataata atgccctttt ctcttcctac agtatcattg aattcatcta ttagaaggtc
     2461 cattgtatcc ttcaatagac ctccgtaatt atgccataaa ttaattgtta cgggattttt
     2521 tgcgcttagc gggtcattgc ttttgcatcc tgttaaaagc attataaaaa tcaatataca
     2581 tacaaccaat tttttcataa aa
//
LOCUS       DAOSOW010000277         2595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_39683_length_2595_cov_3.760630, whole genome shotgun sequence.
ACCESSION   DAOSOW010000277 DAOSOW010000000
VERSION     DAOSOW010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39683_length_2595_cov_3.760630"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..1596)
                     /locus_tag="PLD67_07275"
     CDS             complement(112..1596)
                     /locus_tag="PLD67_07275"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOT22359.1"
                     /translation="MNIKNLLKEMDYLSYIGEDDIEIRGITNDSRKVNRNDIFVAIKG
                     FTNDGHKFIENALENGASAVICENIPENVKGKSNFIIVKSARASMAKAANIIYGRPSE
                     KMKIAGVTGTNGKTSTTYLLNGIYDYLGVKSGIIGTMGVLIEGKKIKVDNTTPEAFDI
                     QHYLAMMLEKDITHCCMEVSSHALELNRIDEVKIDVGIFTNVTRDHLDFHKTMENYYQ
                     AKKKLFYLTKVNNIINVDDPYGLRLYNELIKEGIKAISVGIENDADIKASNLKTTMNG
                     TTFNLNVMGKEKTAHVNTPGTFSVLNSLGALGAAYALGGNIDDIIEALKQIKGVTGRF
                     EILPNKLDCTIILDFAHTPDGLQKVMETINEFAEGRKIVMFGAGGERDLSRRAPMGEI
                     AGKYCDLSILTSDNPRFEDPYEICKEIAEGVKKSNGEYKIIVERDQAIYYAIDKSQKK
                     DVILLAGKSTEPYQDMGTEKVPYDETMLAKRAIKEVERKRGLSS"
     gene            complement(1737..>2595)
                     /locus_tag="PLD67_07280"
     CDS             complement(1737..>2595)
                     /locus_tag="PLD67_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOT22360.1"
                     /translation="LAQIGLDIGKDVFYKYVKSFGFEEQSGIMLNGEATGIVKKPEYM
                     KDVDVVTQAFGHGVAITPIQLINAVSAISNGGNLMVPRIVKQLIDEDGNIIKNFDTEV
                     RRKVISEETSKIMLQIMKDSAEAGTKQAYRPGYRIGGKSGTAQKVIDGKYIEGKFISS
                     FIGVAPVDNPRAVALVMVDEPEMSIGYYGSIVAGPVAADLLENILKYYDVEPVYTEEE
                     LGHVEKQMVTVPNLLGLTVAEATDKLISAGLESNITIEVDAERTVKSQFPKAGEQVVK
                     SSMVTLTLN"
BASE COUNT          677 a          573 c          396 g          949 t
ORIGIN      
        1 attgctatga ataagctacg tgtcattctg agagctttag ctcgaagaat ctcatattat
       61 aaaccctgag atccttcgct gcgctcagga tgacaaaaag gcagctatcg ctcaggatga
      121 caatcctcgc tttctttcca cttcttttat tgctctttta gcaagcatag tttcatcata
      181 aggtactttt tcggttccca tatcctgata aggctctgtg gatttgcccg ctaacaatat
      241 tacatctttt ttttggctct tgtcaatagc atagtatatg gcttggtcgc gctcaactat
      301 tattttatat tcaccgtttg actttttaac tccttccgct atttccttac atatttcgta
      361 agggtcttca aatctgggat tgtcggatgt aagtatgctt aaatcacagt acttgcctgc
      421 aatttcaccc attggggcac gtcttgacaa atctctttct ccgccggctc caaacataac
      481 aatttttctt ccttctgcaa actcatttat agtttccatt accttctgaa gaccgtcggg
      541 agtatgggca aaatcaagta ttatagtaca atcaagcttg ttagggagga tttcaaacct
      601 tccggtaact ccctttattt gttttaaagc ttcaattata tcatcaatat ttccacccag
      661 tgcatatgct gctcccaaag ctccaaggga attaagaaca ctgaacgttc cgggagtatt
      721 tacatgggct gttttctctt tccccattac attaaggtta aaagttgttc cattcatcgt
      781 ggttttcaag tttgaagctt taatatccgc atcattttca atacctacgg atattgcttt
      841 tataccttct tttataagtt cattgtaaag tctcaagcca taagggtcat ccacatttat
      901 aatattgtta acttttgtta aataaaacag ctttttcttt gcttgataat agttttccat
      961 tgttttatga aaatccaaat ggtctcttgt aacattggtg aaaataccca catctatctt
     1021 aacctcgtca attctgttca attcaagagc atgagatgaa acctccatac aacaatgtgt
     1081 aatatccttt tcaagcatca ttgccaaata gtgctgtata tcaaaagctt caggagtagt
     1141 gttgtctact ttgatttttt taccttcaat aagaacaccc atcgttccta ttattcctga
     1201 tttcactcct aaataatcat atattccatt taataaatat gtagtgctgg ttttgccgtt
     1261 agttccggtt actccggcaa ttttcatttt ttcggaaggt cgtccgtata ttatgttggc
     1321 tgctttagcc attgatgctc tcgctgattt aacaatgata aaattgcttt taccttttac
     1381 attttcagga atattttcgc atataaccgc agatgctcca ttttccaaag cattttcaat
     1441 aaatttgtga ccatcgtttg taaatccttt aattgccaca aatatatcat ttctgttaac
     1501 ttttcttgaa tcgtttgtaa tgccccttat ttctatatcg tcttcgccta tatagcttaa
     1561 ataatccatt tctttgagca gattttttat attcataagt atactcctat ttaaatctat
     1621 gatagtatgt gcaattttat cataattaat taaactgcgc aattgattct tgtgacactg
     1681 gggacggtcc tttttgtcac tttggcggga cgctgaggac cttccccaat gtcccattaa
     1741 tttagtgtca gtgtaaccat ggaagattta acaacctgct ctccagcttt cgggaattga
     1801 gattttaccg ttctttcagc atccacttca attgtaatgt tgctttccaa acctgcgctt
     1861 attaatttat ctgttgcttc cgcaaccgta agtcccaaaa gattcggaac cgtcaccatt
     1921 tgtttttcca catggccaag ctcttcttcg gtataaaccg gctctacatc ataatatttt
     1981 aatatattct ccagtaaatc tgcagcgacc ggtcctgcca ctatacttcc gtaatacccg
     2041 atgctcattt cgggctcatc gaccataaca agcgccacgg ctctgggatt atctacggga
     2101 gccacaccta taaacgaaga tataaacttg ccttcaatat atttaccgtc tattactttt
     2161 tgagctgttc ccgatttacc gcctattctg tatcccggtc tgtaagcttg cttagttcct
     2221 gcttctgcgg aatctttcat aatttgaagc ataatttttg atgtttcttc agatattact
     2281 tttcttctta cttctgtatc gaaatttttt ataatatttc cgtcttcgtc aataagctgc
     2341 tttacaattc tcggcaccat taaatttccg ccgtttgaaa ttgccgaaac ggcattaatc
     2401 aattgtatgg gagttattgc aacaccgtgg ccgaaagcct gtgtcacaac atccacatcc
     2461 ttcatatatt ccggcttctt tacaatacct gttgcttctc cattgagcat aatacccgat
     2521 tgctcttcaa acccgaatga ttttacatat ttgtaaaata catcctttcc tatatcaaga
     2581 cctatttgtg caagt
//
LOCUS       DAOSOW010000278         2579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_40023_length_2579_cov_4.068938, whole genome shotgun sequence.
ACCESSION   DAOSOW010000278 DAOSOW010000000
VERSION     DAOSOW010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40023_length_2579_cov_4.068938"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1047
                     /locus_tag="PLD67_07285"
     CDS             136..1047
                     /locus_tag="PLD67_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009298807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HOT22361.1"
                     /translation="MILPSEIRLCEVGLRDGLQNEPKILTTSEKLKLAEGLINAGFKV
                     IELGSFMSPKAVPQMADTDELFRHFNNVEGVELRALVANKKGVERAINCGCGKIKLNV
                     SASKKHNLANINKTPEETVAGFAENVDLALENGLEVSGSISMPFGSPWEGVIPIKDVE
                     EIVEAYLRVGIKEISLSDAAGVATPRQVYDICSSMQKKYPDVKWWLHFHNTRGMAMAN
                     ILASMEAGITNFDSSLGGFGGCPFVPGAAGNVSSEDVVHMMDEMGIKTGIDILKLIEL
                     SRNLQDMLGNEGDSYILKAGRTKDLFK"
     gene            1117..>2579
                     /locus_tag="PLD67_07290"
     CDS             1117..>2579
                     /locus_tag="PLD67_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HOT22362.1"
                     /translation="MSRMSSMDEAYAKLNQDQIDEIEEKFAKADAAQKIFEKWSQEDI
                     DRTIRAIAWKVANTQTFKELVKMSIEESKLGDPVSRENKKYKIRGVLRDALRQKSVGI
                     IEEIPEKGIVKYAKPVGVIACVIPATNPDLTPAGNAIYALKARNAIIFSPHPRAAKTG
                     GRTIELMREGLRQVGAPEDLIQILGQGKAKINRAMSEALNTKCDLVIATGGQGVVRRA
                     YHSGTPAYGVGAGNATMIWDETAKDELYKAAYNTMLSKTSDFGSGCSADGNIVMHESI
                     FEEGINALIEAGGYLLNESEQESIKKIMWDEECHRLPDSVAQSPQRMAQLAGFEIPED
                     RKFLIAMGTGSYTTAYEDVWCREKLTTLLAVHKYEGEFQNAIDIIKAIHEVGGKGHSV
                     GIYSYDEDHIHRLALEAQVGRIMVRQPQSKSNAGSFTNGMPMTSSIGCGTWGGNATSE
                     NVHLKHYMNVTWVSKEIPEDRPTDEELFGEFYEPEREG"
BASE COUNT          916 a          401 c          600 g          662 t
ORIGIN      
        1 tatgcaattt atatttgtta tatagagaaa ttctattgat actatataaa actagtattg
       61 gacatataga tattattaat ataaaataat attaataaca ttgcgtgcat aaataatatt
      121 gaaaggagtg gcattttgat acttccatca gaaataagat tatgcgaggt aggtttgaga
      181 gatgggttgc aaaacgaacc taaaatttta accacatcag aaaagctcaa gcttgctgaa
      241 ggtctaatta atgcaggttt taaggtaata gaactaggtt catttatgag ccctaaagca
      301 gttccccaga tggcagacac ggacgagctg ttcaggcact ttaataatgt tgaaggcgtg
      361 gaattgcgtg cgcttgtagc caataaaaag ggagttgaaa gggcgataaa ttgcggttgc
      421 ggtaaaatta agctgaatgt ttctgccagt aaaaaacata atcttgcaaa tataaataaa
      481 acacctgagg aaactgtggc agggtttgcc gaaaacgttg accttgcttt ggaaaacggc
      541 cttgaagttt caggctccat atccatgcca ttcggttctc cttgggaggg agtaattcct
      601 attaaggatg tagaagaaat tgttgaagct tatttaagag taggaataaa agaaatttcc
      661 ctttcagatg ctgcaggtgt ggcaacacca aggcaggttt atgacatatg ctcttcaatg
      721 caaaagaaat acccggatgt aaaatggtgg ctgcactttc acaatacaag aggaatggct
      781 atggccaata ttttagcttc aatggaagca ggaataacaa actttgattc atccttagga
      841 ggctttggag ggtgtccttt tgttcccgga gcagcaggta atgtatcttc ggaagatgtt
      901 gttcatatga tggacgaaat gggaataaaa acaggaattg atatattgaa gcttattgag
      961 ctttcgagaa acctgcagga tatgcttgga aacgagggag actcatatat tctaaaagcc
     1021 ggcagaacta aggatttatt taaatagaat tatatattaa tctaaattgt ttaaactatt
     1081 ctaaaatata gttttctata taatatagga ggaaatatga gcagaatgag ttcaatggac
     1141 gaggcttatg caaaacttaa tcaagaccaa attgacgaaa tagaagaaaa gtttgcaaaa
     1201 gctgatgccg cacaaaaaat ttttgaaaaa tggagtcaag aggatattga tagaactatt
     1261 agggctattg catggaaagt agccaataca caaactttta aagaattagt caaaatgagc
     1321 atcgaagaaa gtaaattggg agaccccgta agcagagaga ataaaaaata taaaataagg
     1381 ggagtacttc gtgatgctct cagacaaaaa agcgtaggaa taattgagga aattcccgaa
     1441 aaaggaattg taaaatatgc aaaaccggtt ggggttatag catgtgttat acctgcaact
     1501 aacccggatt taacacctgc agggaatgca atatatgccc ttaaggctag aaatgcaata
     1561 atattttctc cccatccaag agcagcaaag accgggggca gaacgattga actgatgaga
     1621 gagggtttaa ggcaggtagg agctcccgag gatttgatac aaatcctggg acagggaaaa
     1681 gctaagataa acagagccat gtcggaagct ttaaatacaa aatgtgattt ggtaattgca
     1741 accggcggtc aaggtgtagt aagaagggca tatcactccg gcacaccggc ttatggagta
     1801 ggtgcaggaa atgctacaat gatatgggat gaaacagcca aggatgaatt atataaagca
     1861 gcatataata ccatgctttc caaaacatcc gattttgggt cgggatgttc tgcagacggc
     1921 aatattgtta tgcatgagag catttttgaa gaaggaataa atgcattaat agaagcaggc
     1981 gggtatttat tgaatgaaag cgagcaagaa agcattaaaa aaataatgtg ggatgaagaa
     2041 tgccaccgct tgcctgattc ggtcgcccaa tcgcctcaga gaatggcaca gcttgcagga
     2101 tttgaaatac ccgaagacag aaaattcctg atagcaatgg gaaccggaag ctatacaact
     2161 gcttacgagg atgtgtggtg ccgtgaaaaa ttaacaacac ttttggctgt tcataaatat
     2221 gaaggtgaat ttcaaaatgc gattgatata atcaaagcca ttcacgaagt agggggtaag
     2281 ggtcacagcg tgggaatata ttcatacgat gaagatcata tccatagact tgctcttgaa
     2341 gcccaagtag gcaggataat ggttagacaa cctcaatcga aatcaaatgc cggctccttc
     2401 acaaacggaa tgcccatgac atcaagcata ggatgcggca catggggcgg taatgcaaca
     2461 tcggaaaatg ttcatttaaa acattatatg aatgttacct gggtttctaa agaaatacct
     2521 gaggacagac cgactgatga agaattgttc ggagaatttt atgagcctga aagagaagg
//
LOCUS       DAOSOW010000279         2567 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_40276_length_2567_cov_4.156847, whole genome shotgun sequence.
ACCESSION   DAOSOW010000279 DAOSOW010000000
VERSION     DAOSOW010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40276_length_2567_cov_4.156847"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..885
                     /locus_tag="PLD67_07295"
     CDS             31..885
                     /locus_tag="PLD67_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22363.1"
                     /translation="MKECTIYIDVPEKFLKGAGIPNSRFIPSYIYQIKIILKNNSSQK
                     KQCSFSATMGNGIRYLENIKHEGPDINLLDTVSAVEPCKELGNDNVIIFGNNLTLSPN
                     STNTIIFDGALCDKYTINSIENSGDKIPHRSKINFYAHLSDEDKIDDSYFISSTAMDS
                     DMIVKCDDEKLNAGDITKYYVDICTGQYDLARKVYLRSILDAGLEYIGDSCNLEPRNI
                     YEFNGKTIIKWDIGSLQPSEIKRIGYKVKVKNDAKGKLTNKLNSNCINNSTYTQCPVS
                     CKYTIEVC"
     gene            complement(934..>2567)
                     /locus_tag="PLD67_07300"
     CDS             complement(934..>2567)
                     /locus_tag="PLD67_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOT22364.1"
                     /translation="LRNNIAELFAIFLPLGVLVYYHRKLQFDKIHKNYIIKVAVSAVI
                     MQAAVVITVLSSDVGMLSPSYLYSETFLVVESVDKFGLLTTGRLDIKNILKSIVFAVE
                     PEHSEIIESEEPDPADELPGEPQSEEPTIEVAEYNTMDIPFEELIKKEGNEAIRSMHK
                     YFSSVKPTKKNEYTGKFKGKNLILITAEGFSPYAVRKDITPTLYKMQEEGFKFNNFYT
                     PTWGVSTSDGEYVACTGLLPKEGIWSFYKSSKNYMPFAMGNQLKKEGYETKAYHNHYF
                     DYYYRHKSHPNLGYTYKGLGNGLNVTETWPESDLEMIELTVPEYIDSQPFHVYYMTVS
                     GHLQYTFTGNYIAAKNRSLVENLPYSKNVKAYLACNIELDKAMEKLIQELEKKGIAED
                     TLIAISPDHYPYGLTLNEISELAGKEIKSDVDLDRGIFILWSKGMEPVKIDKVCSSLD
                     IIPTLSNLMGLEYDSRLLMGRDIFSDSEPLVMFSDRSFITDKAFYNARTNEVTSFTDE
                     NIESSYIKETAETVINKFKYSSMILDTDYYKIILE"
BASE COUNT          846 a          474 c          424 g          823 t
ORIGIN      
        1 gaattagatt agcagaaagg attgaaataa atgaaagaat gtaccatata catagatgtg
       61 cccgagaagt ttttgaaggg tgcaggtatt ccaaattccc ggtttattcc ctcttatatt
      121 tatcaaataa aaattattct taaaaataac agcagccaaa agaaacaatg ctcctttagc
      181 gctactatgg gaaacggaat caggtatttg gaaaatatca agcatgaagg tcctgacatt
      241 aaccttttag atacagtaag tgccgtcgaa ccatgtaaag aattgggcaa cgataatgta
      301 attattttcg gaaacaatct tactctatcc cccaattcaa caaatacaat aatatttgac
      361 ggagcactat gcgacaagta tacaattaac agcattgaaa attcaggtga taaaatacct
      421 cacagaagca aaatcaattt ttatgctcac ttatcagatg aagacaaaat cgatgactca
      481 tattttattt cgtcaactgc tatggactcc gatatgattg taaaatgcga tgatgaaaag
      541 ctaaatgcag gcgatattac aaagtattat gttgacatat gcacaggaca atatgactta
      601 gcaagaaaag tttatttaag aagtatactg gatgcaggac ttgagtacat aggtgattcg
      661 tgtaatttag aaccgagaaa tatatatgaa tttaacggca aaaccattat aaaatgggat
      721 ataggcagcc ttcaaccttc agaaataaaa agaatcggat acaaagttaa agtaaaaaat
      781 gatgcaaaag gtaaactaac aaataaactt aattcaaact gcataaacaa ctctacctat
      841 actcaatgtc cggtaagctg taaatacact attgaggtat gttgaaagaa aatcaggaga
      901 cgttcaatta aaataaagaa atcgtctcct gttttactct aatattattt tatagtagtc
      961 agtatctaat atcattgatg aatatttaaa tttatttata actgtttccg ctgtttcttt
     1021 aatataggag ctttcaatat tttcatcggt aaatgaagtt acctcgtttg ttctggcatt
     1081 gtaaaatgct ttgtccgtaa tgaagctcct gtccgaaaac ataactaagg gctctgaatc
     1141 cgaaaagata tcccttccca taagaagcct tgaatcatat tccaacccca ttaaattcga
     1201 caaggtagga attatatcca aacttgaaca aaccttgtct attttaacag gctccattcc
     1261 cttgctccaa agaataaaaa ttcctctatc caaatcaaca tctgatttga tttctttacc
     1321 ggcaagctcg cttatttcat ttaatgtcaa accgtaagga taatggtcag gacttattgc
     1381 tatgagagta tcttcggcaa tccctttttt ctccagttcc tgtatcagtt tctccattgc
     1441 tttatccaac tcgatattac atgccaagta tgcctttaca tttttggagt atggcaaatt
     1501 ttccaccaga cttctgtttt tagcagctat atagttgcct gtaaaggtat actgcaaatg
     1561 gccgctaact gtcatatagt acacgtggaa aggttgagaa tctatgtatt ctggtaccgt
     1621 taactcaatc atttccaaat ccgactcagg ccaggtttct gttacattaa gaccgtttcc
     1681 caaaccttta tatgtgtaac ctaagttagg atgagattta tgtctgtaat aataatcaaa
     1741 ataatggtta tgataggctt tggtttcata accttccttt ttaagctgat ttcccattgc
     1801 aaagggcata tagtttttgg aagatttata aaagctccat atgccttcct ttggcaaaag
     1861 tcccgtacaa gctacatatt caccgtcgga ggtagagact ccccaggtag gagtataaaa
     1921 gttattaaac ttaaaacctt cctcttgcat tttgtataaa gtaggcgtta tatcctttct
     1981 gactgcatat ggcgaaaagc cttccgcagt aatcaaaatc aaattcttgc ctttaaattt
     2041 acctgtatat tcatttttct ttgtcggctt gacgctgctg aaatacttat gcatacttct
     2101 tattgcttca tttccttctt tttttatcaa ttcttcaaaa ggaatatcca tagtattgta
     2161 ttctgcaacc tcaattgtcg gttcttcgga ttgaggctct ccgggtagct catctgcagg
     2221 gtccggctct tcagattcaa ttatttccga atgctccggc tcaacggcaa atacaatgct
     2281 tttcaatata ttttttatgt ctaatcttcc tgttgttaaa agtccgaact tatcaacgga
     2341 ttctacaacc aaaaaggttt cggaatataa atatgatggg ctgagcatac ccacatcaga
     2401 agaaagcacg gttataacaa ctgcagcctg cattattact gcagatacag caacttttat
     2461 gatatagttt ttatgtattt tatcaaattg taacttcctg tgataataaa ccaatacgcc
     2521 aaggggaaga aatattgcaa acaattcggc aatattgttt cgaagtg
//
LOCUS       DAOSOW010000280         2562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_40383_length_2562_cov_1.783008, whole genome shotgun sequence.
ACCESSION   DAOSOW010000280 DAOSOW010000000
VERSION     DAOSOW010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40383_length_2562_cov_1.783008"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="PLD67_07305"
     CDS             <1..357
                     /locus_tag="PLD67_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT22365.1"
                     /translation="KRNLSLVMVSHSIGVIREITDYVYIMYAGMIAEEGPTNLVLRNP
                     THPYTVALMECIPKLTGTGISNGIEGRTPDYVNPPKGCRFSPRCKFAFEKCKKHNPEL
                     VETEQGHFVACFKMEA"
     gene            362..1354
                     /locus_tag="PLD67_07310"
     CDS             362..1354
                     /locus_tag="PLD67_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006443109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT22366.1"
                     /translation="MDEKEKILEIKGLKKYFPLRNKKFVKAVDGVDLEIYKGETLGLV
                     GESGSGKSTIAYMVVGMYSATEGKIIFEGEDLTKNGVKRRKKQKGAIQIVFQDPGSSL
                     NPRKRIKKSIDLSLSLHNNIERSKHDDKICELLEMVGLPTEFSEKFPKAIGGGERQLV
                     SVARALAANPSLIILDEPTSALDVSVQAKVIKKLMDLQKEIGLSYLFITHDLSLMRNV
                     AKRVAILYLGKVCELAPTTEFFQKPLHPYTNMLLSSIPVATYEEEAYRPNRIESTGEI
                     PSPVDVPPGCAFHKRCIDCMEICTKEPPRMIEVYPGHQVCCHKYTKPFENMNEV"
     gene            1357..2379
                     /locus_tag="PLD67_07315"
     CDS             1357..2379
                     /locus_tag="PLD67_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOT22367.1"
                     /translation="MDKKKMTAGKLLERHFMAKKGEAIVITLDTLSNMEAAELIAEAG
                     HKMGLKVITVNMPAPTGVGMEVDGQIAGECIKGALNQADIWIELNKKWIFGSSTYSNI
                     MGMNRKLRHMCLTGADMLLLENCIGEVNYDVLIPFANRLVDIIKDANTMRMTTKMGMD
                     ISFKNVKGRAVTCELGSANVPGSFMLPGQIAWTPDIDTIQGKLVFDGALAPVCGVPKK
                     PVKIEIVNGKVNSVTGGEEAAKYKNWLESFGHPQMLRISHTGLGLNPGAILSGDVIQD
                     QRVWGSATWAFGSIGSNLVEKAVDAPSHSDCVSLGITLYIDGRTIFEDGEVVDSELSE
                     IVRKIK"
BASE COUNT          879 a          381 c          589 g          713 t
ORIGIN      
        1 aaaagaaatt tgtcactggt tatggtatct cattcaatag gtgtaattcg tgaaataact
       61 gattatgtat atataatgta tgccggaatg attgcagaag aaggtccgac aaatcttgtt
      121 ctccgtaatc cgacgcaccc gtatacagtt gcattaatgg aatgtattcc aaaattaaca
      181 gggaccggca tttccaacgg aatagaagga cgtacaccgg attatgtaaa tccgcccaag
      241 ggatgtagat tttcaccgcg ttgcaagttt gcatttgaaa aatgtaaaaa acacaatcct
      301 gaattggtcg aaacagaaca aggacatttt gtagcatgct ttaagatgga ggcttagaaa
      361 tatggatgaa aaagaaaaaa ttttagaaat aaaaggattg aagaaatatt ttcctttaag
      421 aaataaaaaa tttgtaaaag cggtagacgg tgttgacttg gaaatatata agggagaaac
      481 tctgggattg gtaggagagt caggctcagg taaaagtact attgcgtata tggtagtggg
      541 tatgtatagt gcaacggaag gaaagataat ttttgaagga gaagatctta caaaaaatgg
      601 agttaaacgc agaaaaaaac aaaagggtgc tatacagatt gtgtttcagg atccgggatc
      661 ctctctgaat ccacgtaaga ggataaagaa aagcattgat ttatccctaa gcttgcataa
      721 taatattgaa cgctcaaaac acgatgacaa aatatgtgaa ctacttgaaa tggtaggttt
      781 gcctacagaa tttagtgaaa agtttcctaa ggctatcgga ggaggggaac ggcagctggt
      841 atccgtcgca agagcgctag ctgcaaatcc atccctgatt attttggatg aaccgacaag
      901 tgccttggat gtttcggtgc aggcaaaagt aattaaaaag ttaatggatt tgcaaaaaga
      961 aatagggtta tcatatttgt ttattactca tgatttaagc cttatgcgca atgtagcaaa
     1021 gagagttgcc attctttatt tgggtaaagt ttgtgagctg gcaccaacaa cagaattttt
     1081 ccaaaagccg ctgcacccgt ataccaatat gttattatcg tcaattcccg ttgcaactta
     1141 tgaagaagaa gcatatagac caaatagaat agagtctacc ggtgaaatcc catcaccagt
     1201 agatgtaccg ccgggatgtg cattccacaa gaggtgtata gactgtatgg aaatatgcac
     1261 aaaagagcca cctcgcatga tagaagttta tcccggtcac caggtatgtt gtcacaaata
     1321 tacaaaaccc tttgagaata tgaatgaggt gtaataatgg ataaaaagaa aatgacagca
     1381 gggaaactct tggaaagaca ttttatggca aagaaaggcg aagcaatagt aataacactt
     1441 gatacattaa gtaatatgga agctgctgaa ttgattgctg aggcaggtca caagatgggg
     1501 cttaaagtta taacggttaa tatgccggca ccaacaggtg taggtatgga agtggatggg
     1561 cagattgcag gtgaatgtat taaaggggca ttaaaccaag cagacatctg gattgagttg
     1621 aataaaaaat ggattttcgg ttcatccacg tactcaaata ttatgggtat gaaccgaaag
     1681 ctccgtcata tgtgtcttac gggagctgat atgttattac ttgaaaactg catcggcgaa
     1741 gtaaattacg atgtgcttat tccgtttgct aataggcttg tagacattat taaagatgca
     1801 aatactatga ggatgactac aaaaatgggt atggatattt cttttaagaa tgtaaaagga
     1861 agagctgtta cctgtgaact tggttctgca aatgtgccgg gaagctttat gttgccggga
     1921 caaatcgcat ggactcctga tattgataca attcagggta agcttgtttt cgacggggca
     1981 ttggcacctg tatgtggtgt tcccaaaaag cctgtaaaaa ttgaaattgt aaatggaaaa
     2041 gtaaattcag tgacaggtgg agaagaagct gccaaatata aaaattggct tgagagtttt
     2101 ggacatcctc agatgcttag aatttcacat acaggacttg gattaaatcc tggagcaatt
     2161 ttatcaggag atgttattca agaccagcgt gtgtggggat ctgcaacatg ggcttttgga
     2221 agtattggtt ctaatttagt ggaaaaagcg gtagatgctc cttcccactc tgattgtgtc
     2281 agcctgggaa tcacattata tattgatgga agaacaattt ttgaagatgg agaagttgta
     2341 gacagcgaat tgtctgaaat tgttcgcaaa ataaaataga atgaggacat gaatatacaa
     2401 taaattgact tttaaatgag tgaggttaac tacctaagtt ttagtgaaag gaagtgcaat
     2461 tttggattac aaaaagtata tatcatccaa tgttctgtct atgaatttat ctaccataag
     2521 aatatttttt gacttggtaa accaaatgga agaagtcact tc
//
LOCUS       DAOSOW010000281         2548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_40678_length_2548_cov_4.317288, whole genome shotgun sequence.
ACCESSION   DAOSOW010000281 DAOSOW010000000
VERSION     DAOSOW010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40678_length_2548_cov_4.317288"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PLD67_07320"
     CDS             <1..771
                     /locus_tag="PLD67_07320"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOT22368.1"
                     /translation="LLNKEAKAQNKIYGVVLMYDLGDLREGVFYKEELIRLAEYVEYE
                     SDNLYLEGIGSNLSCYGSVAPTTENLSQLAEAATEIEEKLNRKLNIISGGGTTTLPLL
                     VRGGVPKKINHLRIGEGINNTQDLPLYWDTNIEGLDPDVFVLKAQIVEINEKPTHPIG
                     QLMVNAFGDLPHYEDRGIRKRAIIALGNQDLGDSSRLVPKDKNMIVLGASSDHTILDI
                     HDCKTEYKLGDIIEFNILYQAMLMTTLSDYVHKKIIKY"
     gene            804..989
                     /locus_tag="PLD67_07325"
     CDS             804..989
                     /locus_tag="PLD67_07325"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HOT22369.1"
                     /translation="MPIIQVEMLKGRTVEQKREMVKEITEVVSRTANCPKEAVRIVIR
                     EMEFENFGISGKLQCDI"
     regulatory      1138..1239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            1307..2470
                     /locus_tag="PLD67_07330"
     CDS             1307..2470
                     /locus_tag="PLD67_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003502167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOT22370.1"
                     /translation="MKKFLGIILALTMILSLFTACGSNGQQTKPESEGPEVSGEGIFP
                     AIAKEDIKVGVIHIGNPADGSGYTFAHDQGIIEMQKALGLSDSQIIRKNNVNDTDPTA
                     TDAAIRECIEEGAHIIFGTSWGYMDTMEALAEEYPDVIFSHGSGYKSNDRNFNNYFGR
                     IYQARYLTGIAAGLKTKTNKLGYVAAMGKDNSEVTGGLNAFAMGVHSVNPEAKVYVKV
                     TNSWYSPEEETNAAKALIAEGCDVIAQHCDTPNPQLEAEKAGVFGIGYNSDMSKDAPK
                     ATITSAVWNWGAYYTWAVEKVINGTWLSENYYGGMKEGMIDISPINESIAAEGTVEKI
                     EEAKQSIMDGTFNVFDGVIETNDGSTVGEEGKTLDDGTITGGINWYFETVVEK"
BASE COUNT          909 a          387 c          551 g          701 t
ORIGIN      
        1 ttgctaaata aagaagcaaa agctcaaaat aaaatctacg gcgttgtgct tatgtatgat
       61 ttgggggatt taagagaagg agtattttat aaagaagaat tgattcgatt agccgaatat
      121 gttgaatatg aatcagataa tttatatctt gaaggtatag gctcaaattt aagctgttac
      181 ggctcagttg ctccaactac ggaaaactta agtcaattgg cagaagcagc aacagaaata
      241 gaagaaaaac taaacagaaa actaaacata atatccggag gagggacaac cacgctgcct
      301 cttttggtga gagggggagt tccaaagaaa ataaatcatc ttcgtattgg agaaggaata
      361 aataatacac aagatttgcc tctttactgg gatacaaata ttgaaggtct ggaccctgat
      421 gtatttgttt taaaggcaca aattgttgaa ataaatgaaa aaccgactca ccccatcgga
      481 caattaatgg taaatgcttt tggagatttg cctcattacg aagacagggg aatcagaaaa
      541 agggcaatta ttgcattggg aaatcaggat ttaggggact catcaaggct tgttccaaag
      601 gataagaata tgatagtatt aggagccagc agcgaccata ccattttgga tattcacgat
      661 tgcaaaacag aatataaatt aggagatata attgaattta atattttgta tcaagcaatg
      721 ctgatgacca ccttgtccga ttatgtacat aaaaaaataa taaagtatta aatttaccat
      781 attgagaaag aggaggtgga atcatgccca ttattcaggt agaaatgcta aagggaagga
      841 ccgttgagca aaaacgagaa atggttaagg aaataacgga agtagtatca agaaccgcca
      901 attgtccaaa ggaagccgta agaatcgtta taagagaaat ggaatttgaa aactttggaa
      961 taagcggaaa gcttcaatgt gacatttaag actgaccttc taattactga taaattctgt
     1021 atctgagcat tagttagtat tcaactatta atattaaatt atttctgcga accctgtccc
     1081 accgggctga taacaaaata atttgttgat tattgtcaca gcttaagtta taattatgtt
     1141 gaatatatag actgcatcaa tataatatgg ataatatggt tccatagttt ctaccaaaca
     1201 accttaaatt gtttgactat tgatagtatt attctattga tgcgattagg gatacttttc
     1261 gcatttttta tttcttgatg caaagtttat tacataggag gataaaatga aaaaattttt
     1321 aggtattatt ttagcgctga caatgatact ttcattgttt actgcatgcg gttccaacgg
     1381 acaacaaaca aaacccgaat cagaggggcc ggaagtttcc ggagaaggaa tatttcctgc
     1441 aattgccaag gaagatatta aggttggagt aatacatatc ggaaatcctg ctgatggctc
     1501 cggttatacc tttgcacacg atcagggaat aatagaaatg cagaaagcac ttggtcttag
     1561 cgacagccag ataattcgta aaaacaatgt taatgataca gaccctacag ctactgatgc
     1621 agctatacgc gaatgtattg aagaaggagc acacatcatt ttcggtacaa gctgggggta
     1681 catggataca atggaagcct tagccgaaga atatcctgat gtaatatttt cacacggttc
     1741 agggtataaa agcaatgata gaaactttaa caattatttc ggaagaatct atcaggcaag
     1801 atatttaaca ggaattgcag ccggattaaa aacaaaaact aataagttgg gatatgtggc
     1861 agcaatgggt aaggataatt cagaggtaac aggcggacta aatgcatttg caatgggtgt
     1921 gcattcagtt aaccctgaag caaaagttta tgtaaaggtt acaaacagct ggtattcacc
     1981 cgaagaagaa acaaatgccg caaaagcatt gatagctgaa ggctgtgacg taatagccca
     2041 gcattgcgat actcccaacc ctcaattgga agcagagaag gcaggagttt ttggtatagg
     2101 ctataattca gatatgtcta aggatgctcc aaaggctacc attacatctg cagtatggaa
     2161 ttggggagct tattatacat gggctgtaga aaaagttatt aacggaactt ggttaagtga
     2221 aaattattat ggtggaatga aggaaggcat gattgatatt tcacctatta atgaaagtat
     2281 tgctgcagaa ggaaccgttg aaaaaatcga ggaagcaaaa caatctatta tggatggcac
     2341 cttcaatgtt tttgacggag taatagagac taatgacggc tccacagttg gagaagaagg
     2401 caaaaccttg gatgacggta ctattaccgg cggaatcaat tggtattttg aaactgtagt
     2461 agaaaaataa ttcaatgggg ttggttgttg ccaacccctt aatacagcgt cattttgatg
     2521 gcaatagccg aaatctcatg cggtatta
//
LOCUS       DAOSOW010000282         2522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_41226_length_2522_cov_3.820835, whole genome shotgun sequence.
ACCESSION   DAOSOW010000282 DAOSOW010000000
VERSION     DAOSOW010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41226_length_2522_cov_3.820835"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..672
                     /locus_tag="PLD67_07335"
     CDS             136..672
                     /locus_tag="PLD67_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOT22371.1"
                     /translation="MNIGENLKRLRVANSLTQQELADRCELTKGYISQLERDLTSPSI
                     ATLTDILECLGTDLGSFFNEAVDSKIVFKQDDVFVKESEDNYIINWIVPNAQKYKMEP
                     ILVELGSGSKTKTDEPHEGEEFGYVLNGSILLNYGNQVYKVKKGESFYIKANKTHYIE
                     NVGKSKARVLWVSTPPYF"
     gene            685..1725
                     /locus_tag="PLD67_07340"
     CDS             685..1725
                     /locus_tag="PLD67_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT22372.1"
                     /translation="MNNAIIQLNNITKKFDGEIVLDDITLSVKENEFVTFLGPSGCGK
                     TTTLRIIGGFETPDEGEVYFDGKLINDVPPFKRQLNTVFQKYALFPHLNVFENIAFGL
                     RIKKMDEKTISEKVEKMLELFNLKNYGKRRIDQLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRKEMQHELKAMQKQLGITFIYVTHDQEEALTMSDTIIVMNEGIIQQMG
                     TPIDIYNEPENAFVADFIGESNILDGIMIDDYLVEFLGKRFECLDSGFGRGEEVDVVI
                     RPEDLDLTPAGEGKLEGIVKSVTFKGVHYEMDIVANNFEFLVHSTDMAPVGTTVGLTL
                     TPDDIHIMEKGE"
     gene            1728..>2522
                     /locus_tag="PLD67_07345"
     CDS             1728..>2522
                     /locus_tag="PLD67_07345"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT22373.1"
                     /translation="MKNRTKYFAYPYVLWIFLFIALPAFLVLLYSITTKESNGLTTIH
                     FTLENFKKFFQPMYLNILWDSIYLAAISTIICLILGYPAALIISRAHVSKRNTLLFLC
                     ILPMWMNMLLRTYAWMTILGNNGILNNIFAFLGLPRFNMLYTRGATVMGMVYNFIPFM
                     ILPIYTALSKMDKALLEAADDLGADKRTVFRKVIFPLSMPGVISGIIMVFMPAVSTFI
                     IPQLLGGNKSMMIGNLVEKLFILNGDWNFGSAISIVMMLIILLSMSI"
BASE COUNT          840 a          373 c          525 g          784 t
ORIGIN      
        1 ataattataa ctttgttgaa gcgaaaaata ataaaaaatt attattgaca gaaggggtgt
       61 ggatatgtat aataaaagct gtttcagtgt ttagtaattt taaactttga gtttagtaaa
      121 aaatgtgagg tttatatgaa tattggagaa aatttaaaaa ggttgagagt tgccaattct
      181 ctcacacagc aggaattggc tgacagatgc gaattgacaa aggggtatat ttcgcagctt
      241 gagagagatt tgacttctcc ttccatagca actcttacgg atatattgga gtgtctgggg
      301 acggatttag gaagcttttt caatgaagcc gtggacagta aaattgtgtt taaacaagat
      361 gatgtatttg taaaagaatc agaggacaat tatattatta actggattgt tccaaatgca
      421 cagaaatata aaatggagcc catattggtg gaactcggaa gcggcagcaa aacaaaaaca
      481 gatgagcctc atgaaggcga agagttcggt tatgttttaa acggctccat acttttaaac
      541 tacggcaatc aagtgtacaa ggttaaaaag ggagagtctt tttacattaa ggcaaacaaa
      601 actcattata ttgaaaatgt gggaaaaagc aaagcgcgtg tactatgggt aagtactccg
      661 ccgtattttt agcaggaggt aagaatgaat aatgcaatta tacaattaaa taatattacc
      721 aaaaaatttg acggagaaat tgttcttgac gatattacct tatccgtgaa ggaaaatgaa
      781 ttcgtaactt ttttgggacc aagcggctgt ggaaaaacaa ctactcttag aattatcgga
      841 ggctttgaaa ctcctgatga aggtgaagtt tattttgacg gcaaattaat caatgacgtt
      901 cctcctttta aaagacagct gaataccgtg tttcagaaat atgctttgtt tcctcattta
      961 aatgtttttg aaaacatagc atttggtctg agaataaaga aaatggatga aaagaccatt
     1021 tccgaaaagg ttgaaaaaat gcttgagctt tttaatctca aaaattacgg caaaagaagg
     1081 attgaccagt taagcggagg acagcagcag cgtattgcaa tagcaagagc tttggtaaat
     1141 gagcccaagg tacttttgct cgatgaaccc ttaggtgctt tggatttaaa attaagaaaa
     1201 gaaatgcagc atgagctcaa agcaatgcag aaacagcttg gaataacatt tatttatgta
     1261 actcatgacc aggaagaagc tttaacaatg tcagacacga ttatagtcat gaatgaaggc
     1321 attattcaac aaatgggtac tcccatagat atatataatg agcctgaaaa tgcgtttgtg
     1381 gcagatttta tcggagaaag caatatattg gacggaatta tgatagatga ttacttggtt
     1441 gaatttttag gaaagagatt cgaatgtctc gattcaggat ttggaagagg cgaagaagtt
     1501 gatgtggtta ttcgccctga agatttagat ttaactcctg ccggtgaagg aaagctggag
     1561 ggaattgtaa agtcggtaac atttaaaggt gttcattatg aaatggacat agtagcaaat
     1621 aattttgagt ttttggttca ttcaaccgat atggcccctg taggaacaac tgtcggttta
     1681 accttaactc ctgacgatat tcatataatg gaaaaggggg aatagctttg aaaaacagaa
     1741 caaaatattt tgcttacccc tatgtcttgt ggatattttt gttcattgct ttaccggcat
     1801 ttttggtgct tttatacagc attaccacaa aagaatctaa cggacttaca acgattcatt
     1861 ttacattaga aaatttcaag aaattttttc aacccatgta tttgaacatt ttatgggact
     1921 ccatctactt agcggcaatt tcaaccataa tatgtttgat actcggatac cctgcggcat
     1981 taataatttc tagggcacat gtgtcaaaga gaaatacttt gttgtttctt tgtattttgc
     2041 ccatgtggat gaatatgctc cttagaactt atgcttggat gaccatactt ggaaacaatg
     2101 gaattttaaa taacattttt gcttttttag gtcttccgag gtttaacatg ctttatacaa
     2161 ggggcgctac cgtaatgggt atggtttaca actttattcc ctttatgata ctgcccattt
     2221 atactgcatt aagtaaaatg gataaagccc ttttggaagc agccgacgat ttgggagcag
     2281 acaaaagaac agtttttaga aaagttattt tccccttaag catgcccggt gtaatttcgg
     2341 gcatcataat ggtttttatg cctgcggtaa gcacatttat cattcctcaa ctcttaggag
     2401 gaaataaaag catgatgata ggaaacttgg tggaaaaatt gtttatttta aacggagact
     2461 ggaattttgg ctcagccata tccattgtaa tgatgcttat aattctttta tccatgtcaa
     2521 tt
//
LOCUS       DAOSOW010000283         2522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_41227_length_2522_cov_3.481962, whole genome shotgun sequence.
ACCESSION   DAOSOW010000283 DAOSOW010000000
VERSION     DAOSOW010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41227_length_2522_cov_3.481962"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            36..923
                     /locus_tag="PLD67_07350"
     CDS             36..923
                     /locus_tag="PLD67_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOT22374.1"
                     /translation="MANIFSMTGYGKGEHNDGKRSITAEIKAINNRYCDINIKTPRHL
                     RIYEDNIRKILKNGIQRGRIDVYINIEYISESDTVILPNLALAKQYMDAIEKIKSELG
                     INGNPSLDTIIKFQDVLIAKEDDADDDELRICLENAMNEAVKNLLTMRVKEGIQLKDD
                     ISLSLVKIRELMEVISENSHTIVEDYKIKFEDRIKELLGNTHELDENRLYNEIVIYAD
                     KSDINEEIVRFNSHMVQLSDALDSGGAIGRKLDFILQEANREINTIGSKVSSLDIIQV
                     VIEIKNLLEKIREQVQNIE"
     gene            933..1181
                     /locus_tag="PLD67_07355"
     CDS             933..1181
                     /locus_tag="PLD67_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HOT22375.1"
                     /translation="MSNTINIGFGNIALANRIVAIVSPESAPIKRLIQETREKNKLID
                     ATYGRKTRAVIITDCDYIILSAIQPDTMALRFENHNIK"
     gene            1191..1820
                     /gene="gmk"
                     /locus_tag="PLD67_07360"
     CDS             1191..1820
                     /gene="gmk"
                     /locus_tag="PLD67_07360"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOT22376.1"
                     /translation="MDEGLLVVISGPSGAGKGTICAQLLLEMSYLKVSVSVTTRKPRA
                     GEVDGISYYFIEEEEFINRINNDEFLEYAKVYGNYYGTPKEEVFKQLKAGNDIILEID
                     IQGALQVKKNYPMGVFIFILPPSLEELKSRIEGRGTDSKEVILRRMESAYEELNYAFQ
                     YDYVVVNDHVDTATEKIKHIILAEKNRVIRKKEIISKIREIKNEKNINR"
     gene            1798..2085
                     /gene="rpoZ"
                     /locus_tag="PLD67_07365"
     CDS             1798..2085
                     /gene="rpoZ"
                     /locus_tag="PLD67_07365"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF013365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOT22377.1"
                     /translation="MKKISIDKLNDMVDSQYSLVTIISKRARQIIDGSEPLIKTKTKK
                     PVSIAIEEFYERKFEAIYNYDKYKKSLTANKETAVETTDEKKDMDETTAQE"
     gene            2139..>2522
                     /locus_tag="PLD67_07370"
     CDS             2139..>2522
                     /locus_tag="PLD67_07370"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22378.1"
                     /translation="MYNTFVQVILNNISKSTDKIYTYGVPDALKSFVDEGKRVRINFG
                     RGKNIIDALIVSVDLTCDYPIEKIKPILDIIDEKPIVTKEMIKILFWIRERYLCKYSD
                     AMRLMIPSETKSIYEKHIRLKEDKEI"
BASE COUNT         1017 a          329 c          510 g          666 t
ORIGIN      
        1 aagtaggaaa ataattaaaa taattggaga ataaaatggc aaatatattt agtatgaccg
       61 gttacggcaa aggtgaacac aatgacggta aaagaagtat tacagctgaa ataaaagcta
      121 tcaacaacag gtattgcgat ataaatataa aaacccccag acatttgcgt atttatgagg
      181 ataatataag aaaaatcttg aaaaacggca ttcaaagggg tagaatagat gtatatatta
      241 acattgaata tataagtgaa tcagatacag taattttacc gaatttggcc ctggctaaac
      301 agtatatgga tgccattgaa aaaattaaat cagaattagg aattaacggc aacccatctc
      361 ttgacacaat aatcaaattt caggatgttc ttattgcaaa ggaagatgat gccgatgatg
      421 atgaactccg tatttgtctg gaaaatgcaa tgaatgaggc tgtaaaaaac ctgctgacaa
      481 tgagagttaa agaaggtata cagttaaagg atgacattag cctaagcctg gtaaaaatac
      541 gtgaattaat ggaagtcata tctgaaaact cccatacaat tgtggaggat tataaaatta
      601 aatttgaaga caggattaaa gagctgttgg gaaatactca cgagcttgat gaaaacaggc
      661 tttacaatga aattgtaatt tatgccgata aaagcgatat taatgaagaa attgtaagat
      721 ttaactcgca tatggtgcaa ttgtccgatg ctttggacag cggaggtgca ataggaagga
      781 agcttgattt tatcttacaa gaagctaaca gagaaattaa taccataggc tctaaagtca
      841 gcagcttaga tattatacaa gttgttattg aaataaagaa tcttttggaa aaaatcagag
      901 agcaagtaca aaatattgaa taggagttaa atatgtcaaa tactattaat attggttttg
      961 gtaatattgc gctggcaaac agaattgtgg caattgtaag tcctgaatcg gcaccgataa
     1021 aacgccttat tcaggaaacc cgtgaaaaaa ataaacttat agatgcaact tacggcagaa
     1081 aaacaagagc tgtaattatt actgattgcg attatataat tctatcggca attcaaccgg
     1141 atacaatggc actgagattt gagaaccaca atatcaagta gaggtgagtt atggatgaag
     1201 gattattagt ggtaatatca ggcccttcgg gagcaggcaa aggcacaatt tgtgctcagc
     1261 ttctgttgga aatgagttac ctgaaagttt ccgtatctgt tacgaccaga aagccaaggg
     1321 caggcgaggt agacggaata agctattatt ttattgaaga agaagaattc ataaatagaa
     1381 taaataatga cgaatttctt gaatacgcaa aagtttacgg caattattac ggtacgccca
     1441 aagaagaagt gtttaagcag cttaaagccg ggaatgatat aatattagaa atagatattc
     1501 aaggagcact acaggttaaa aagaattatc cgatgggagt gttcatattt atccttcccc
     1561 cgtcactgga agaattaaaa tccagaattg aaggaagagg tactgattca aaagaagtga
     1621 ttctaagaag aatggagtca gcttatgagg aattaaacta tgctttccaa tatgattatg
     1681 tggtggtgaa tgaccatgtg gatactgcaa cagaaaaaat taaacatata atattggcag
     1741 aaaaaaacag agtaataagg aaaaaagaaa taatcagtaa aatcagggag ataaagaatg
     1801 aaaaaaatat caatagataa attaaatgat atggttgaca gccagtattc attagtgaca
     1861 ataatatcaa aaagggcaag acagataatt gacggctcgg agcctttaat taaaacaaag
     1921 actaaaaagc ccgtgtcaat cgctattgag gaattctatg aaagaaaatt tgaagccata
     1981 tacaattatg acaagtacaa aaaatcattg acggcaaata aggagacagc tgttgaaaca
     2041 acagatgaaa aaaaagacat ggatgaaaca acggcacagg aatagtcaga taatcagtca
     2101 aaggactgat tatttaattt tagttgagcg gagaaaatat gtataatact tttgtacaag
     2161 ttattttaaa taatatttcc aaaagcacag acaaaattta cacatacgga gtaccggatg
     2221 cattgaaaag ctttgttgat gaaggaaaaa gggtcagaat taatttcggc agagggaaaa
     2281 atattattga tgcattaata gtatccgttg atttaacctg tgattatccc attgaaaaaa
     2341 ttaagcctat tttagatata atagatgaaa aacctattgt aacaaaagaa atgattaaaa
     2401 tccttttttg gataagggaa aggtatttat gtaagtattc cgatgccatg agattgatga
     2461 taccttccga aacaaaaagt atttacgaaa aacatatcag actgaaagag gataaggaaa
     2521 ta
//
LOCUS       DAOSOW010000284         2502 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_41673_length_2502_cov_3.570903, whole genome shotgun sequence.
ACCESSION   DAOSOW010000284 DAOSOW010000000
VERSION     DAOSOW010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41673_length_2502_cov_3.570903"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            294..1037
                     /gene="aroD"
                     /locus_tag="PLD67_07375"
     CDS             294..1037
                     /gene="aroD"
                     /locus_tag="PLD67_07375"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HOT22379.1"
                     /translation="MKNTRIKNINLNGMPKICVPVTGVTENEIIDEFSKLKNAPIDMV
                     ELRADYFEFVQDQTKVTDILEKIRKLYEKPLLFTLRTKNEGGVSYIDADYYFKLNNSV
                     IESKLVELIDIEFFSQAEKVDKTVKLARKNNVMTILSNHDFLKTPPEEEIISRVNKMM
                     DCGDIAKIAVMPNSEEDVLTLLSAALKLKKEKKGHFIAISMGPLGVISRISCELIGSC
                     ITYASYGDKSAPGQLDVRLIKELLKIMHI"
     gene            complement(1034..2338)
                     /locus_tag="PLD67_07380"
     CDS             complement(1034..2338)
                     /locus_tag="PLD67_07380"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT22380.1"
                     /translation="MKNKIIIIGVVFIFLIAASYLIPQEVPMINIDDSIKMYIVADPH
                     LMSDKLTEDSEVFNKYLNTLDRMMKYTGVFLDIIEDDVKKNQPDIVVFPGDLTNNGSK
                     VNHLEFEKRLKRIKSTGTKVYVVPGNHDINNEKALYFKDNEIHITESINKEEFVEIYK
                     SYGYNEAISRDENTLSYLVKPYKNLWLLMLDTTKDYPEPGGYINRDTLNWIASCSDMA
                     KEENAEIIVVMHHNLLDHSDIIWEDYTVDNNTSVINTFHECDIQLVLSGHIHIQDVKT
                     HTSVETGNTIYDIATSSLPVFTHQYGQLTYSPSRGYDYETIKLNVEEYALKNNIEDEN
                     LLSFNEYAEKFFVENLCKTHKDSIGLLEIPDEHKEEVFKTVTEMNKKYFSGYRNEALK
                     QLIETEGFKIVEELDECFIQEYIMGMIKDQNADNNKLHIPIN"
BASE COUNT          799 a          420 c          390 g          893 t
ORIGIN      
        1 tttatattga caaaccttat ataaatttag taatctttga aatatattgc tcccgattta
       61 tataattata ttactatata caatgcattg tcaattataa atattgcaat ttcaacattt
      121 tttgggacaa tgttttcatt gttgtctttc tttaattctt attatataat aatacaatcg
      181 atgagcaaag accttgcctt tgcaccaatg tataatagta tcagcgtggc aagcctatat
      241 caacttgaat taatgataaa ataaataaca ataataaaat ttggagaatt tacgtgaaaa
      301 atacccgaat aaaaaatata aatttaaatg gtatgccgaa aatttgtgtg ccggtaaccg
      361 gtgttacaga gaatgaaatc attgatgaat ttagcaagct aaaaaatgct cccatagata
      421 tggttgagct tagagcggat tattttgaat ttgtacagga ccagacaaag gtaacagata
      481 ttcttgagaa aataagaaaa ttatatgaaa aacctcttct gtttacattg cgaacaaaaa
      541 atgaaggcgg ggtttcatac atagatgctg actattactt taaactaaac aacagtgtaa
      601 tcgaaagcaa gttggttgag cttatagata ttgaattttt ctctcaagcc gaaaaagtag
      661 ataaaaccgt taaattagca agaaaaaata atgtgatgac aatattgtcc aaccatgatt
      721 ttttaaaaac tcctccggag gaagaaataa tctcaagggt taataagatg atggattgcg
      781 gagatattgc caaaattgca gtgatgccta attctgaaga agatgtactg acactgcttt
      841 ctgcagccct taaattaaag aaagaaaaaa aaggccattt catagccatt tctatggggc
      901 ctcttggagt tatttcaaga atatcatgtg aattaatcgg ttcctgcata acctatgctt
      961 cttatggcga taagtcggca ccgggtcaat tagatgtcag attaattaaa gaactgttaa
     1021 aaatcatgca tatttagttt atgggaatat gcaatttatt gttgtctgcg ttttggtcct
     1081 taatcattcc cattatatat tcttgtatga agcattcatc taattcttca acgattttaa
     1141 acccttcggt ttctataagc tgcttcaatg cctcatttct gtatcctgaa aagtattttt
     1201 tattcatttc ggttaccgtt ttgaatactt cttctttatg ctcgtcggga atttccaaca
     1261 gacctatact atctttatgt gttttgcata aattttcaac gaaaaacttc tcggcatatt
     1321 cattaaatga aagtaaattc tcatcttcga tattattttt taaagcatac tcttccacat
     1381 tcagcttgat tgtttcataa tcatagcctc ttgacggaga ataggtcaac tgtccgtact
     1441 ggtgggtaaa tacgggcagg ctgctcgtag ctatatcata tattgtattg cctgtttcta
     1501 cggatgtatg tgttttaaca tcttgaatat gtatatgtcc agacaagaca agctgtatat
     1561 cgcactcatg gaatgtatta attacgctgg tgttgttgtc caccgtatag tcttcccata
     1621 taatatcgct gtggtcaagc aagttgtggt gcattacaac tattatttcg gcattttctt
     1681 cctttgccat atctgaacat gatgcaatcc aatttagtgt atctctgttt atataaccgc
     1741 ccggttcagg gtaatccttt gtcgtatcaa gcattaacag ccacaaattc ttgtaaggct
     1801 taactaagta gctgagtgta ttttcgtctc ttgatattgc ttcattatat ccgtatgatt
     1861 tatatatttc cacaaactct tctttgttta tactttcagt tatgtgtatt tcattatctt
     1921 tgaagtataa tgctttttca ttattaatat catggtttcc cggaactaca tatacctttg
     1981 tacccgttga ttttatcctt tttaaacgct tctcaaattc caagtgatta accttggagc
     2041 cgttatttgt taaatctccc ggaaaaacaa caatatcggg ctgattcttt ttaacatcat
     2101 cttctataat atcaaggaaa acaccggtat atttcatcat tctgtcgagg gtgttcaaat
     2161 atttgttaaa tacttcgcta tcttctgtca atttgtcaga cattaaatga gggtcagcaa
     2221 ctatgtacat cttaatacta tcatcaatat taatcatagg tacttcctga gggatgaggt
     2281 aggatgcagc aattaaaaat ataaaaacaa ctcctattat gataatctta tttttcattg
     2341 ctccatcctc cggttatata attctattta tttcattata caataaaatc aaaaaaataa
     2401 caatgttttt attttttagt tttccaataa aatggcataa aaattggaac acccctctat
     2461 tatggagtgc cccattccta ttctaaagta taaatcagct at
//
LOCUS       DAOSOW010000285         2494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_41858_length_2494_cov_2.886839, whole genome shotgun sequence.
ACCESSION   DAOSOW010000285 DAOSOW010000000
VERSION     DAOSOW010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41858_length_2494_cov_2.886839"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1292
                     /locus_tag="PLD67_07385"
     CDS             180..1292
                     /locus_tag="PLD67_07385"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOT22381.1"
                     /translation="MLYITKNHLKPGMVLAKDIYLYNRRNFNTFLLAKGQVLSSTYIN
                     RINFHNIDGVYIENDAFSDINVEPYIADDLKAESLQRISDTYYEFQVSSGKINDYMIN
                     RLSKTVKNIIIELLNKDDLSYNIMDFKNYDSYTFQHCLNVAMLSISTGISLKLSEQDL
                     LDLGLAGMLHDIGKMLIPIEILNKPSTLTKEEFEIIKTHPVNASKQLRNMVPPEVLRA
                     VEDHHERLDGTGYPHEKRDNNISLYARILSISDVYDSLISDRPYRKSIFPNEVVEYLM
                     GCADKQFDYNILKHFLKIIFAYPVGTFVKLSNEELAVVIKNHSENILRPIVRIIKNDG
                     TVGEDVDLLYDKDYMNITIIDMGYDLLSMYPLADSY"
     gene            complement(1384..2298)
                     /locus_tag="PLD67_07390"
     CDS             complement(1384..2298)
                     /locus_tag="PLD67_07390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HOT22382.1"
                     /translation="MKINKIALIGMGAVGTVYGSLLYNKYGSNFTVISDESRKDKLKK
                     NGFTLNGNTFYPNVSSNSDNTIKYDLIIFAVKNYQLAGALEDVRSFVGENTILITFLN
                     GVTSRDIIQEAYPNNKVLYGLAMRIDAVRNEAGVVNTEDGEIHFGEADNRVISEEVQA
                     VKDCFDAAGIRNIVFPDMIRMYWKKFMLNVGFNQVTAVTRAPYGKVTNIETSLILFKE
                     AMNEVLTIAKALNIDLREEDIEEFVALMDHFSPFGKTSMLQDVEAKRKTEVDYFGGTV
                     VELGKKLNIPTPVNHVLYCIIKSLEELY"
BASE COUNT          820 a          455 c          392 g          827 t
ORIGIN      
        1 tttagcttcg caacctgcta ttccaacaca aaaatccagt cccaacaatt cacttaatac
       61 tataatcggt gctgtccttg gaggtatggt tggagtcatg gcagcattat ttatggaata
      121 ctggtttaaa aaagaaaata attaatattt ctatacaata ttactgcttg gagaattcta
      181 tgttatatat cactaaaaat caccttaagc cgggtatggt attggcaaaa gatatttatc
      241 tatataaccg aagaaatttt aatacttttc ttttggctaa gggacaagta ctcagcagca
      301 cttacatcaa cagaatcaat tttcacaata ttgatggagt ttatatagaa aacgatgcct
      361 tttctgatat caatgtggaa ccctatattg ctgatgactt aaaggcagaa tcactgcaac
      421 gcattagtga cacatattat gaattccaag taagttcggg gaaaataaac gattatatga
      481 ttaaccggct gtcaaagacc gttaaaaaca ttataataga gttattgaat aaagatgatt
      541 tatcatataa cattatggat tttaaaaact atgacagcta cacatttcag cattgcctga
      601 atgtggctat gctaagcatt tctacgggaa tatcattgaa actcagcgag caagaccttc
      661 ttgatttagg tttggccgga atgctgcatg atatcggtaa aatgttaata cccattgaaa
      721 tacttaataa gccgtcaaca ctgacaaaag aagagtttga aattataaag acacatcctg
      781 tcaatgcatc aaagcaatta agaaatatgg tcccacctga agttttaaga gcagttgaag
      841 accatcatga aagacttgac ggcacaggat atccacatga aaaaagagac aataacataa
      901 gtctatacgc aaggatattg tcaataagcg acgtgtacga ttctttaatt tcagacaggc
      961 catatagaaa atctatcttt ccaaatgaag ttgtagagta tttgatggga tgtgccgaca
     1021 aacaatttga ttataatatt ttaaaacact ttttgaaaat tatttttgcc tatccggtag
     1081 gaacatttgt taaactaagc aacgaagaat tagcggtagt aattaaaaat cattccgaaa
     1141 atatattaag accaatagtg cgaattataa aaaatgacgg cactgtaggc gaagatgttg
     1201 atttacttta tgataaggat tacatgaata tcacgataat tgatatggga tatgacctgc
     1261 tttctatgta tccccttgct gattcttatt aataagtttc caacagtaaa aaagggcgat
     1321 attcaattag tcgcccatgt ttgttttagc agttccgcat aaagttagtt atattcttaa
     1381 tgcttaatat aattcttcta atgatttaat tatgcaataa agcacatggt taacaggcgt
     1441 aggtatatta agcttcttgc ccaattcaac aaccgtccct ccgaaataat caacttcagt
     1501 ttttctctta gcctcaacat cctgcagcat ggatgttttg ccaaagggtg aaaagtggtc
     1561 cattaaggca acaaattcct ctatatcctc ttccctcaaa tcaatgttta atgctttcgc
     1621 aatggtcaga acttcattca ttgcttcctt aaacaatatt aaactcgtct caatgtttgt
     1681 caccttgccg taaggagccc ttgtaactgc cgtaacctgg ttaaatccca cattgagcat
     1741 gaattttttc caatacatcc ttatcatatc cggaaataca atgtttcgta ttcctgcagc
     1801 atcaaagcag tcctttacag cctgcacttc ttcagaaatt actctattat ccgcttcccc
     1861 aaaatgaatt tctccatctt cagtatttac tactccggct tcatttctta cagcatctat
     1921 tctcattgcc agcccataaa gaaccttatt attcggataa gcctcttgaa ttatatccct
     1981 tgaagttacc ccgtttaaga atgttattaa tattgtgttt tcacccacga aacttcttac
     2041 atcttcaaga gcaccggcta gctgataatt ttttaccgca aaaattatta aatcatattt
     2101 aatggtatta tcgctgtttg atgatacatt tggataaaat gtgtttccat tcaatgtaaa
     2161 tccgtttttc ttcaacttgt cttttcttga ttcatccgat ataacagtaa aattcgaacc
     2221 atacttgtta tagagcagac ttccgtacac ggtgccaacc gcacccattc ctattaatgc
     2281 aattttgttt attttcatgt tcatcaccta taatatttat ttaatttcat agttattgat
     2341 tataataaag ttatccttta aatgcaatga ataaatttat tttactatat ttttgaaatg
     2401 tatgttataa tgaatacgaa aaatgaagca aagactttgg tctttgcact gatgtattca
     2461 ttgtatcggt gtgaagcaag ccgattttgc ggct
//
LOCUS       DAOSOW010000286         2490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_41959_length_2490_cov_3.584394, whole genome shotgun sequence.
ACCESSION   DAOSOW010000286 DAOSOW010000000
VERSION     DAOSOW010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41959_length_2490_cov_3.584394"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /gene="amrB"
                     /locus_tag="PLD67_07395"
     CDS             complement(<1..1116)
                     /gene="amrB"
                     /locus_tag="PLD67_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOT22383.1"
                     /translation="MMIVNSFIVPHPPLIIPEIGRGEEIEIQKTIDAYHNIAKQISEI
                     KPDTIIITTPHSIMYSDYIHISPGKRAKGSFREFGQRDVTMEVEYDVELVGEISNEAE
                     KAGISAGTLGEKNSSLDHGVMVPLYFVNKYYNNYKLVRISISGLSYLEHYKFGKCIKK
                     AAEKSNKKIVFIASGDLSHMLKETGPYGYREEGVIFDREVTKAMDSGNFSEFLKFDED
                     FCEAAAECGLRSFIIMAGALDGKKLSSKLLSYQGPFGVGYAVAEFKVMGDDEGRRFDE
                     IYLKTEMERIESLKSEEDEYVRLARQTLESYVINRKVIKRPDNLSPELIKNKAGVFVS
                     LKLDGSLRGCIGTISPTTSSIADEIIRNAISAGTEDPR"
     gene            complement(1178..2428)
                     /locus_tag="PLD67_07400"
     CDS             complement(1178..2428)
                     /locus_tag="PLD67_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOT22384.1"
                     /translation="MDKSKGKLTKLERSWILYDVGNSAFILLISTIIPIYFKNIASSD
                     GVLPSDSTAYWGYAMSTSTLIVAILGPVLGALADTKGYKKPLFTFFLMSGVLGCAALS
                     LPVPWIIFLALFVFARVGISGSLIFYDAMLPDVTSDERMDLLSSHGYAWGYIGSCIPF
                     TVSLLLILFADKIGIATALATGIAFILNALWWFLISLPLLKNYKQLHFVEVDNAPMAM
                     AFRRLGRTFGDIKKHKEIFIFLLAFFFYIDGVDTIIGMATSYGKDVGISDNNLLLALL
                     LTQVVAFPFAIIFGKLSKRYKTSELISVCIIGYFLITLFALQLDKAWEFWFLAVCVAV
                     FQGAIQALSRSYYAKIIPKESSSEYFGFYDIFGKGASFTGTTLMGITTQITGNSKFGV
                     LTICIMFIIGYVIFRKAVKMQTVS"
BASE COUNT          749 a          545 c          396 g          800 t
ORIGIN      
        1 cctcgggtct tctgtaccgg cacttattgc attccttatt atttcatcag ctatgcttga
       61 tgttgtcgga gaaattgttc caatgcagcc tcttaagctg ccgtctagtt tcaaggatac
      121 aaatactcct gccttgtttt taataagttc agggctcaaa ttgtccggtc tcttaattac
      181 ctttcggttt attacatagc tttccaatgt ttggcgggca aggcgcacat attcatcctc
      241 ttctgatttc aatgactcaa tcctttccat ttcggttttt aagtatattt catcaaatcg
      301 acgcccttca tcatctccca taaccttaaa ttctgcaact gcatagccta ctccaaaagg
      361 tccttgataa gacagaagct ttgagcttaa ttttttcccg tccaatgcgc ctgccattat
      421 tatgaaggag cgaagtccgc attcagcagc agcttcacag aaatcttcgt caaatttaag
      481 aaactccgag aaatttccgc tatccattgc cttggttact tccctgtcaa atatcactcc
      541 ctcttccctg taaccgtaag ggcctgtttc cttaagcatg tgagacaaat ccccgcttgc
      601 tataaaaaca atttttttgt tgcttttttc tgctgccttt ttaatgcatt ttccaaattt
      661 ataatgctct aaataagaaa gtcctgatat ggaaatgcgc accaacttat aattattgta
      721 gtatttattc acgaaataaa gaggcaccat aacaccgtgg tcaagcgagc tgtttttctc
      781 acccaaagtt ccggcagaaa tcccggcttt ctctgcttca tttgaaattt caccgactaa
      841 ctcaacatca tattctactt ccattgtaac atcacgttgt ccaaattccc ggaagctgcc
      901 ttttgccctc ttccccggtg aaatatgtat ataatctgaa tacattattg aatggggagt
      961 tgttataatt attgtgtcag gtttaatttc agaaatttgt tttgcaatat tgtgatatgc
     1021 atctattgtc ttttgaattt caatttcttc tcctcttcct atctcaggaa ttatcaatgg
     1081 aggatgcgga actataaaac tattcacaat catcataatt tctcctttct ttcttatatg
     1141 attgctatga ataacatatg tcattccaaa agctatctca tgagacagtc tgcattttta
     1201 ctgcttttct gaatattaca tatcctatta tgaacataat acaaatggtc aatacaccga
     1261 attttgagtt gcccgtaatt tgtgtcgtaa ttcccataag cgttgttccg gtaaaggatg
     1321 cacctttacc aaaaatatcg taaaaaccga agtattcgct ggagctttcc tttggaatta
     1381 tttttgcgta atatgagcgg gacaaagctt gtatagctcc ctggaaaacc gctacgcata
     1441 ctgccagaaa ccaaaattcc caagctttat caagctgcag cgcaaacagt gtaattaaaa
     1501 aataacctat gatgcacacg ctgattagct ctgaagtctt atacctttta gacagcttgc
     1561 caaatattat tgcaaaagga aaagcaacca cctgtgttaa aagaagcgcc aataatagat
     1621 tattgtcact tattccaacg tccttgccgt atgatgtggc cattcctata attgtatcta
     1681 ccccgtctat ataaaagaaa aaggcaagca agaatatgaa tatttcctta tgttttttta
     1741 tatcgccaaa tgttcttcca agccgcctaa atgccatagc catgggagca ttatccactt
     1801 ccacgaaatg aagctgcttg taatttttaa gaagaggcag gcttattaaa aaccaccata
     1861 atgcatttag tataaaagct attcccgttg caagggcggt ggcaattcca attttatctg
     1921 caaataaaat caataaaaga cttacggtaa aaggaataca gcttccaata taaccccagg
     1981 cataaccgtg tgaagacaat aaatccatcc tctcgtcact tgttacatcc ggaagcatgg
     2041 catcgtaaaa tatcagactt cctgaaatac ctacccttgc aaatacaaac aaggctaaaa
     2101 atattatcca cggaacggga agtgatagtg ctgcgcaccc aagtaccccg gacattaaaa
     2161 aaaaggtaaa caaaggtttc ttatatcctt ttgtatctgc taatgctcct aatacaggtc
     2221 ctaatatcgc aactatcaaa gttgaagtcg acatggcata tccccaataa gcagtcgaat
     2281 cggaaggtaa aacaccgtcc gatgaagcaa tatttttgaa atagataggt ataattgttg
     2341 atatgagcag aataaatgca gaattcccga catcatacaa aatccaggac ctttccaatt
     2401 tagttaattt tcctttagat ttatccataa tctccacctt aattgtatat ttttctttag
     2461 ttacattttc ggtgtgtcac catacctttt
//
LOCUS       DAOSOW010000287         2484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_42086_length_2484_cov_3.433923, whole genome shotgun sequence.
ACCESSION   DAOSOW010000287 DAOSOW010000000
VERSION     DAOSOW010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42086_length_2484_cov_3.433923"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2484
                     /locus_tag="PLD67_07405"
     CDS             <1..>2484
                     /locus_tag="PLD67_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HOT22385.1"
                     /translation="DGEILYSYYGSNEGNPLNSALDAVKGLYSQLHSGIKIAYTAVTG
                     YGERLIKAALNVDIGEIETVAHYKAADFFLKGVDFIIDIGGQDMKSMVIKNGVIDSIM
                     LNEACSSGCGSFIETFAKSLDMTVEEFAQEATWSKNPVDLGTRCTVFMNSKVKQAQRE
                     GATISDISAGISFSVIKNALFKVIRLNNLDSLGEKIVVQGGTFYNDSVLRAFEQLTGR
                     EVVRPDIAGIMGAFGAAVIAKNKYSEEKESIFVKAADLDNFSFNTSMERCGLCSNNCL
                     LTVNKFSDGRYYVSGNRCERGAEKYISITKKESLPNLYKYKTKRVFNYKPLPLSEARR
                     GIIGIPRVLNMYEDFPFWFTFFSELKYRVVLSGISSKKIYELGMGTIPSESVCYPGKI
                     AHGHITDLINKKVKKIFYPCIFYNIKEDESAGNHYNCPIVVSYPESIEANIDMKDVVF
                     YKPFLNLNDKERLIEKLYRELGEAEGLTLFEIRSAAEKAYEELFSYKQDVRNEGERAL
                     TYISENNIKGIVLAGRPYHIDPEINHGIPEMIESYGLAVLSEDSISDKGTVERPLRVI
                     DQWSYHTRLYKAATFVSNTDMLELIQLNSFGCGLDAVTTDQVREILEKNNKIYTVIKI
                     DEITNLGAVRIRLRSLLASMEEREKNGVVHKYSPSNFKRVIFTKEMKENHTILIPQMS
                     PIHFQFISTAAKMSGYRAVVLPAVDKKAVDAGLKVVNNDACYPSIIVIGQLINALTSG
                     KYDLNNTSLMISQTGGGCRATNYIGFIRKALKDSGYGDIPVISFNVVGLEKQPGFKLT
                     APFFNRLIMAIVIGDTLMRTTYRTRPYEKI"
BASE COUNT          834 a          413 c          511 g          726 t
ORIGIN      
        1 gacggggaaa ttctttattc ttattacgga agcaatgaag gaaatcctct gaattctgcc
       61 cttgatgcag ttaaaggatt atattctcaa ttacattcag gcattaaaat tgcatatacg
      121 gcagtgaccg gttatggaga acgcttaata aaggctgcat taaatgttga tataggagaa
      181 attgaaaccg ttgctcacta taaagctgca gatttctttt taaaaggcgt ggactttatt
      241 attgacatag gcggccaaga tatgaaaagt atggttatta aaaatggagt tattgattcc
      301 attatgctta atgaggcatg ctcctcaggc tgcggctcct ttattgaaac atttgcgaaa
      361 tctctggata tgactgttga agaatttgcg caagaagcaa cttggtctaa aaatcctgta
      421 gaccttggca caagatgcac tgtatttatg aattcaaagg taaagcaagc tcaaagagaa
      481 ggagcaacca taagcgacat ttctgccgga atttcatttt cggtgattaa gaatgcctta
      541 tttaaagtaa taagacttaa taacctggat tcattgggag aaaagattgt tgttcaggga
      601 ggaacatttt acaacgattc tgttcttcgc gcatttgagc aattaacggg acgagaagtt
      661 gtaagacctg acattgcagg tattatggga gctttcggtg ctgccgtgat tgccaaaaat
      721 aaatatagcg aagaaaaaga aagtattttt gttaaagcag ccgaccttga taatttttct
      781 ttcaatacat ccatggagcg ctgcggtctt tgctcaaaca attgcttatt aactgtcaat
      841 aaattttccg acggcagata ttatgtatcc ggcaatcgct gtgaaagagg tgctgaaaag
      901 tatatttcaa ttactaaaaa ggaatctttg ccaaacctgt acaaatataa aaccaagcgt
      961 gtttttaatt ataaaccgct gcccttgagt gaagcaagaa gaggaatcat cggcattcct
     1021 agggttctta atatgtatga agattttccc ttctggttta catttttcag cgaacttaaa
     1081 tacagggttg tgctttcagg aatatcaagc aagaaaattt atgagcttgg aatgggaaca
     1141 ataccatccg aatcagtttg ttatcccggt aaaattgcac atggacacat aaccgacctt
     1201 attaataaaa aggtaaaaaa aatattctat ccttgtattt tttataatat taaggaagat
     1261 gaaagtgcag gcaatcacta taactgtcct attgtagtat cttatccgga atcaatcgaa
     1321 gctaacattg atatgaagga tgtagtattt tacaagccat tcttgaatct gaacgataag
     1381 gagagactca ttgaaaaact ttacagagaa ttaggagaag ctgaaggttt aactctgttt
     1441 gaaatccgtt cagctgcaga gaaagcttat gaagaattgt tctcttacaa gcaggatgta
     1501 agaaatgagg gggaaagagc actaacatat ataagcgaga ataatataaa aggtattgtt
     1561 cttgcaggaa gaccctacca tatagatccg gaaatcaacc acggcatacc tgaaatgata
     1621 gaaagttacg gtttagctgt attgtcagag gattccatca gtgataaagg cactgttgaa
     1681 agaccccttc gtgttattga ccaatggtct tatcatacaa gactttacaa agcggcaaca
     1741 tttgtttcaa atactgatat gttggaatta atccagctta attctttcgg atgcggcttg
     1801 gatgccgtaa caaccgacca ggtaagggag atacttgaaa aaaacaataa gatatacacg
     1861 gtaattaaaa tagatgaaat tactaacctt ggggcagtca gaattcgttt gcgctcattg
     1921 ctggcttcta tggaggaaag agaaaaaaac ggagttgttc ataagtattc tccctcaaac
     1981 ttcaaaaggg ttatctttac aaaagaaatg aaggagaatc atactatact gatacctcaa
     2041 atgtctccca ttcattttca atttattagt actgctgcaa aaatgtccgg ttatagggct
     2101 gttgttcttc cagcagtaga caaaaaagcc gtggatgcag gactgaaagt cgtaaacaat
     2161 gatgcctgct atccttcaat tatagtaata ggacaactta ttaatgcttt gacatcggga
     2221 aaatacgatt taaacaatac ctcgcttatg atttctcaga caggaggagg ctgccgtgct
     2281 acaaattata taggatttat aagaaaagca ctgaaggact caggttacgg agatattccg
     2341 gtaatatcat ttaatgtggt aggtcttgaa aagcaaccgg gatttaagct gactgctccc
     2401 ttttttaaca gacttataat ggctatagta ataggggata ctctgatgcg taccacttat
     2461 cgcacaaggc cttatgaaaa aata
//
LOCUS       DAOSOW010000288         2484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_42098_length_2484_cov_2.439275, whole genome shotgun sequence.
ACCESSION   DAOSOW010000288 DAOSOW010000000
VERSION     DAOSOW010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42098_length_2484_cov_2.439275"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PLD67_07410"
     CDS             complement(<1..292)
                     /locus_tag="PLD67_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT22386.1"
                     /translation="MDKKLLEVKNLKTYFYSDGVIIKSVDDLSFYVNEGETLGIVGES
                     GCGKSVACLSLARLVETPPGKYEYGEILFKGEDLLRVSDARIRQIRGNDISFI"
     gene            complement(297..1181)
                     /locus_tag="PLD67_07415"
     CDS             complement(297..1181)
                     /locus_tag="PLD67_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT22387.1"
                     /translation="MAKDKTNNNYTGNQSYWGQVLSRLRKNKMAMISLYILIIIIALC
                     IIIPIISPYEIQTTNLSGKDQKPNSRHLLGTDLIGRDIFTRLFYAGRISLGIALATVI
                     IECVIGVVLGSLSGFYGGIIDSIIMRITEMFMSFPFMIICITVVAVFGNSIPNLILVL
                     AILSWPNIARIVRGQILVLREMEYMEACEALGISDLRRIFKHLLPNVSAYVIVYATLG
                     MASVILVETSLSFLGLGISPPTPTWGNMIQEARNILIIQQKWWYWVPPGTAIFISVMC
                     FNILGDGIRDALDPKMMR"
     gene            complement(1192..2157)
                     /locus_tag="PLD67_07420"
     CDS             complement(1192..2157)
                     /locus_tag="PLD67_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT22388.1"
                     /translation="MRYYILRRIIQIIPVLIGIIFILFFILEQAPGTPVSYMMHPRMT
                     PEQKAELEEKLGLDIPWYERFVNYIKEASQGNLGYSSTHKKPVTEVIGNYAGPTLLLA
                     LVSLTISIFIGIPAGIISAVKQHTVIDNILTVMSFIGISIPSFFFGLLLLKAFAVDIQ
                     LFPLFGLKDPLLMSDNIFVKIKDIAWHLVLPSIVLGLNSTASFMRYTRSSMLEVIKQD
                     YIRTARAKGLKEKTIIYRHAFRNGIIPLITLLGFWIPLLLSGAIVTESIFALPGLGKI
                     SVEAAMSRNYPLILGINSMLAILTLIGALVADILYALADPRIRYD"
     gene            complement(2226..>2484)
                     /locus_tag="PLD67_07425"
     CDS             complement(2226..>2484)
                     /locus_tag="PLD67_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT22389.1"
                     /translation="MAGGFNASGYKNAQAMDLVRAGKKEFDIEKRAEIYKEWAKIMNE
                     EIPHAIIAYRSEIWGINKRVDGMFIDTYATWDLSLKNVTLK"
BASE COUNT          752 a          512 c          423 g          797 t
ORIGIN      
        1 aaataaaaga tatatcattt cctcttattt gtcttattct tgcatctgaa accctaagca
       61 aatcttcacc cttaaaaagt atttctccgt attcatattt ccccggcggt gtttccacca
      121 agcgtgccaa agacagacag gctacgcttt tcccgcatcc cgattcgcct actataccca
      181 aagtttctcc ttcattaacg taaaagctta aatcgtcaac tgactttata ataactccgt
      241 ctgaataaaa atatgttttc aggtttttaa cttccagtaa ttttttatcc ataaaattac
      301 ctcatcatct tggggtctaa ggcatcgcgt atgccgtcgc ccaatatgtt aaagcacata
      361 acagatatga aaatagctgt tcccggagga acccagtacc accacttctg ttgaattatt
      421 agtatattcc ttgcttcttg aatcatgttt ccccaggttg gagtaggagg agaaataccc
      481 agacctaaaa agcttaaaga agtctccact aagattacac ttgccatccc aagtgttgca
      541 taaacaatta cataggctga tacattaggc aaaaggtgtt taaatattct tcttaggtca
      601 ctgatgccaa gagcttcgca ggcctccata tattccattt ctctaagaac aagtatttgt
      661 cctcttacaa tacgtgctat gtttggccaa ctcaatatag ccagtactaa aattagattc
      721 ggtatactgt tcccgaatac tgctacaacg gtaatgcata ttatcataaa aggaaatgac
      781 ataaacattt cggtaatacg cattattatc gaatcaatta ttcctccgta aaagccggat
      841 aaactgccca aaacaacacc tattacacat tcaattataa ccgttgcaag tgcaatgcca
      901 agtgatattc ttcctgcata aaacagtctg gtaaaaatat ctcttccaat taaatccgtg
      961 cctaaaaggt gccgggaatt aggtttttga tctttgcctg agagattcgt tgtttgaatt
     1021 tcatagggtg atataattgg aataataatg catagggcaa ttattattat aagaatgtaa
     1081 agacttatca tcgccatttt gttttttctt agtcttgaga gtacttgtcc ccaataggat
     1141 tgatttccgg tataattatt atttgtttta tccttagcca tatgtacctg cctaatcgta
     1201 tcttattctc gggtctgcaa gagcatacag tatgtctgca accaaagctc ctatcagtgt
     1261 aaggatcgca agcattgaat tgatacctaa aatgagggga tagtttcttg acattgctgc
     1321 ttcgacagat atttttccaa gtccgggcaa tgcaaaaata ctttccgtta ctatcgctcc
     1381 cgataaaagt aaaggtatcc agaaaccaag caatgtaata agggggatta ttccgtttct
     1441 aaatgcatgt ctgtaaataa tggttttttc tttcagaccc ttggctcttg cagtcctaat
     1501 ataatcctgc ttaataactt caagcatgct agagcgggtg taacgcataa agcttgcagt
     1561 tgagtttaag cctaagacaa tactcggcaa tactaaatgc caggcaatat cttttatttt
     1621 aacaaaaata ttgtcagaca ttaacaaggg gtctttcagc ccaaacagcg gaaagagctg
     1681 tatgtctacg gcaaaagctt ttaacagcaa gaggccaaag aaaaacgacg gtatgcttat
     1741 gccgataaaa gacatcaccg ttaatatatt gtctattacg gtatgttgtt ttacagcact
     1801 tattataccg gcgggaatac cgataaaaat agaaattgtt aaagagacta aggcaagcag
     1861 cagggtaggt cccgcataat ttccgataac ttcggttaca ggcttcttgt gggtgcttga
     1921 atagcctaaa ttaccttgag atgcctcctt tatatagttg acgaatcttt cataccatgg
     1981 aatgtccaag cctaattttt cctccagttc ggctttttgc tcaggagtca ttctcggatg
     2041 catcatatat gaaaccggag tacccggtgc ttgttcgagg ataaaaaata atataaatat
     2101 aattcctatg agtacaggaa ttatttgaat tatgcgcctc aaaatataat atctcataat
     2161 accgcctctt ttataatgat atagtctaag gtcaataaat tagggcagga ctgcctgccc
     2221 ctttgttatt ttaaagtaac gttcttcaaa gataagtccc atgttgcata ggtatcaata
     2281 aacattccat ctaccctttt gtttattccc caaatttcac ttctgtatgc tatgattgcg
     2341 tgaggaattt cttcattcat tattttagcc cattctttat aaatttcggc acgcttttct
     2401 atgtcgaatt ctttctttcc tgctcttaca aggtccattg cttgggcatt cttataaccc
     2461 gatgcgttaa atccgcctgc cata
//
LOCUS       DAOSOW010000289         2473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_42360_length_2473_cov_2.457403, whole genome shotgun sequence.
ACCESSION   DAOSOW010000289 DAOSOW010000000
VERSION     DAOSOW010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42360_length_2473_cov_2.457403"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..475)
                     /gene="trmL"
                     /locus_tag="PLD67_07430"
     CDS             complement(5..475)
                     /gene="trmL"
                     /locus_tag="PLD67_07430"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HOT22390.1"
                     /translation="MSLNIVLFEPEIPQNTGNIARTCAATGSKLHLIKPLGFSVNDKY
                     LKRAGLDYWDLVDITYYENFEEFKKTTGNNKVFISTTKESRFYTEVAYSDECYIIFGK
                     ETAGLPGYIHEEYIKNRIKIPMVQKVNARSLNLANSVNIIVYEALRQLGFPQMI"
     gene            complement(557..1195)
                     /gene="nth"
                     /locus_tag="PLD67_07435"
     CDS             complement(557..1195)
                     /gene="nth"
                     /locus_tag="PLD67_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008680233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOT22391.1"
                     /translation="MMTKTEKVYDLLKKAYPDAKCGLVFNTPLQLLISTILSAQATDK
                     SVNKVTEELYMQYPDLDSLLTLSHEEISLKIKQIGLYRTKSKNIYNMLRVIKNNYKGE
                     VPKNMDDLMSLPGVGRKTASVVLAEAFNIPAFPVDTHVFRVTRRIGIAKENTADKVSD
                     EMMKKLPKYKWHLMHHLLITHGRTTCTAKSPKCTICNVNEICTYYKKVVKEK"
     gene            complement(1192..1473)
                     /locus_tag="PLD67_07440"
     CDS             complement(1192..1473)
                     /locus_tag="PLD67_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HOT22392.1"
                     /translation="MIVCSCEIEILIYESNSLKEKRQVIKSIIERIKSKFNASVAEVG
                     YNELWNRSLIGIAVVSNNKRVCDESISKIVKFIDYDERVEILSCNKEII"
     gene            complement(1470..>2473)
                     /gene="queG"
                     /locus_tag="PLD67_07445"
     CDS             complement(1470..>2473)
                     /gene="queG"
                     /locus_tag="PLD67_07445"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOT22393.1"
                     /translation="ELLTNRKINNQDCEFEEQDIKKRINAKNLFPELKSIIAIGVPYG
                     EGYKLPSCRNKGLLSVSCHGEDYHKTVNSLLVKLAEEIMRHKSFKFLPCVDTSYLIDK
                     EICRSAGIGSYGKNSLLINKNYGSFINLGYLLTDIETETDIISEKDICGTCNLCVKSC
                     PNKAIFEIGGINPKKCISYLTQTKNYIPLEYRENMGRQIYGCDICQIVCPLNKEVLNK
                     EVNTVYSDLIVDLEELLNISNKGFINKYGHTAGSWRGRNIWKRNALISIGNLNIKSLY
                     QNVKDELQNQSEIIRCYAAWSLLKLNRPLAEEILYSNLKYENDYVKNEYLKLLEAKL"
BASE COUNT          716 a          456 c          328 g          973 t
ORIGIN      
        1 cctcctatat catctgtgga aatcccaatt gtcttaaagc ttcataaaca attatattaa
       61 cagaattagc cagatttaat gaccttgcat tcaccttttg aaccattgga attttaatcc
      121 tgttttttat atattcttcg tgaatataac cgggaagacc tgcagtttct tttccaaaaa
      181 ttatataaca ctcatcagaa taagctactt ctgtataaaa cctgctctct tttgttgttg
      241 aaataaatac tttattattt ccggttgttt tcttaaattc ttcaaagttt tcataatatg
      301 taatatcgac caaatcccaa taatcaagtc ctgcacgttt caagtattta tcatttaccg
      361 aaaaacctaa tggttttatt aaatgaagtt ttgagcctgt agctgcgcat gttcttgcaa
      421 tattacccgt attttgcggt atttcaggct cgaataaaac tatatttaat gacatttatt
      481 attttctcct acgttattaa ttaaatagac attccctgat tataataagg cataatttta
      541 gtaaatacaa gaaaatttac ttttccttta ctactttttt ataataagta catatttcat
      601 ttacattgca gattgtacat ttaggagact tcgcagtgca tgttgttctc ccgtgtgtta
      661 ttaacagatg gtgcatgagg tgccacttat attttgggag ctttttcatc atttcatccg
      721 atactttgtc tgcagtattt tcctttgcaa ttcctattcg cctggttact ctaaaaacat
      781 gagtatctac aggaaaggca ggtatattaa aagcttcggc caaaactacg gaagcagtct
      841 ttcttccgac acccggcaga gacatcagat catccatatt tttaggcact tctcccttat
      901 aattgttttt tatcactcgc agcatattgt aaatattctt agattttgtt ctatataatc
      961 ctatttgttt aatttttagt gaaatttctt catgagataa agttaataga ctatccaaat
     1021 caggatattg catgtacaac tcctctgtta ccttattaac acttttatct gtagcctgtg
     1081 cacttaaaat tgttgaaatt aacagctgaa gaggggtatt aaaaacaagt ccgcactttg
     1141 catcaggata agcttttttt aacaaatcgt acactttttc cgtcttggtc attatattat
     1201 ctccttattg cagcttagta tttccactct ttcatcatag tctataaatt tgactatctt
     1261 tgatattgat tcatcgcata ctcttttatt gtttgataca actgcaattc ctatcaagga
     1321 cctgttccat aattcattat atccgacttc tgctacggaa gcattgaatt ttgatttaat
     1381 tctttctatt atacttttaa ttacttgtct tttttccttt agggaattag attcatatat
     1441 caatatttca atttcacatg agcaaactat cataattttg cctccaatag ttttaagtat
     1501 tcatttttta cataatcatt ttcgtatttt aaattactat atagtatctc ttcagccaag
     1561 ggtctgttta attttaataa agaccaagct gcgtagcacc ttattatctc cgactgattt
     1621 tgaagctcat ctttaacatt ttgatacaat gatttaatat taagattccc tatagaaata
     1681 agagcatttc tcttccatat atttctgccc ctccagctgc ctgcggtgtg tccatattta
     1741 tttataaaac ctttgtttga tatattcagc aattcttcca aatcaacaat taggtctgaa
     1801 taaactgtat ttacctcttt attcagaacc tctttattta aagggcagac aatttggcat
     1861 atatcgcagc catatatttg tcttcccata ttttctcggt attccaaagg tatatagttt
     1921 tttgtctgag ttaaataaga aatgcatttt tttggattaa tacctccgat ttcaaatatt
     1981 gccttgttag gacagctttt tacacataga ttgcatgtac cgcatatatc cttttcgctt
     2041 ataatgtctg tttctgtttc aatatcagtc aaaagatagc ctaaatttat aaatgagccg
     2101 taatttttat ttatgagcag gctgtttttc ccataacttc ctatacctgc acttctgcat
     2161 atttctttat ctatcagata tgatgtgtca acacagggaa gaaatttaaa acttttatga
     2221 cgcattattt cttcagccag cttaaccaat aaagaattaa ccgtcttatg gtaatcttct
     2281 ccatgacatg atacgcttaa taaaccttta tttctgcatg aaggaagttt gtaaccttct
     2341 ccgtaaggta caccaatagc aattatgctc tttaattcgg gaaagagatt tttggcattt
     2401 attctttttt ttatatcctg ctcttcaaat tcgcagtcct gattattgat ttttctgttt
     2461 gttaaaagct ctt
//
LOCUS       DAOSOW010000290         2466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_42514_length_2466_cov_3.208212, whole genome shotgun sequence.
ACCESSION   DAOSOW010000290 DAOSOW010000000
VERSION     DAOSOW010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42514_length_2466_cov_3.208212"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..2352
                     /locus_tag="PLD67_07450"
     CDS             127..2352
                     /locus_tag="PLD67_07450"
                     /inference="COORDINATES: protein motif:HMM:NF021355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HOT22394.1"
                     /translation="MKNTYKIILLTLILILCFTVTSFAQNEIKVYVDNERVDFDVEPI
                     IENGRTLIPLRGVFEKLGARVDWNKNLLEVVIKDDNNEIEMILGKDKVMVNGIIKDID
                     VPTRMINSRTFAPLRFIAENLGHTVRWDGTTSSVYITKNDTFPVEKGISTVGTKENLI
                     ALLEYNSKLHNYIWMGRDIVVDFAEDSITEAQEAPKAEAESAAPGMDSSDTNVQVEGV
                     QEGDIIKNDGKYIYINTNRGLKIIDSNPDAPKIVYTVPVPENTSISEIFTAGNKLIVI
                     GQNNLFYIMDKSAIDSIDMLEPSIMPPRYYQDRTDVLIYNIENIEKPVLEREYLFDGN
                     YLSGRVIDDSFYLINNKYLNLGYRYYDNKDIDIPLPYYTDVLNYEDYKFGYDEIKYFP
                     NYIDTRYMYIIGINLNDTKSKPDVDVYLGGTDTIYVSKENLYAAVADYSYDYIVRPLE
                     EFSPEYTTSTVIYKFGLKDGKIGTAVQGEVPGTIINQFSMDEHNNTFRIATTTGDMWM
                     NTSKNNVYILDEDLKVLGKLEGLAEGERIYSTRFAGDRMYMVTFRQVDPLFVIDTSNP
                     RAPELKGMLKIPGYSTYLHMVDENHILGFGYDTEVNQWGGTVNGGLKISMFDVSNVNK
                     PKETFTEIIGKSGTYSELLYNHKALMFSLNKGLMAFPVNRTAENYKSDFSGAYVYNVS
                     NDSISLKNTITHKESEFDSYGDNIKRIIYIGDYLYTFSENKMQIHGINTNKKVNELII
                     K"
BASE COUNT          902 a          348 c          494 g          722 t
ORIGIN      
        1 ggggggacac caataaagat ggcagatgaa accatctttt tatatgttta taattagaat
       61 tatttggtat aattaaggaa caagatattg ctaaaatacg tcttatgaaa agagaggtga
      121 gaataaatga agaatacgta taaaattatt ttattaacgt taattttaat tttatgtttc
      181 actgtaacat catttgctca aaatgaaata aaggtttatg tggataatga aagagtggat
      241 ttcgatgttg agcctattat agaaaatggc aggacattga taccattgag gggtgttttt
      301 gaaaagctgg gagccagggt agactggaac aaaaaccttt tggaagtggt tattaaggat
      361 gacaacaatg aaatagaaat gatattgggc aaggataaag taatggttaa cggtattatc
      421 aaggatattg atgtgcctac aagaatgata aacagcagga cctttgcccc ccttagattt
      481 atagccgaaa acttagggca tactgttagg tgggatggaa caacaagctc ggtatacata
      541 accaaaaatg atacttttcc tgttgaaaaa ggcatttcaa ctgttggaac aaaggaaaat
      601 ttaattgctt tattggagta taattcaaaa ctccacaatt atatctggat gggcagagat
      661 attgttgtcg attttgctga agattccatt actgaagctc aagaagcacc aaaagccgaa
      721 gcagaatctg ctgctcccgg catggacagc tcagatacaa atgttcaagt tgaaggtgtt
      781 caggaagggg atattattaa aaatgacgga aaatacattt atattaatac aaacagaggc
      841 ttaaaaataa ttgattcaaa tcctgacgcc ccaaaaatag tttatacagt tcctgtcccc
      901 gaaaatacaa gtatcagtga aatttttacg gcaggaaata agctgattgt aatcggtcag
      961 aacaaccttt tctatattat ggataaatcg gcaattgaca gcattgatat gcttgagcct
     1021 tccataatgc cgccgagata ctatcaggac agaaccgacg tattgattta taatatagaa
     1081 aatattgaaa aaccggtact tgaaagggaa tatctttttg acggcaacta tttatcaggc
     1141 agggttattg atgacagttt ttatttgata aataacaagt atcttaactt aggatacagg
     1201 tactacgata ataaggatat tgatatacct cttccatatt atacggatgt gttgaattac
     1261 gaggattata aatttggata tgatgaaatc aagtattttc caaattatat agacacaagg
     1321 tatatgtata ttataggaat taatttaaac gataccaaat caaaaccgga tgttgatgtg
     1381 tacttaggcg gtacggatac aatatatgta tcaaaagaaa acttgtatgc agcagtagct
     1441 gattattcat atgattatat agttcgtccg cttgaggaat ttagccctga atatactaca
     1501 agtacggtaa tttataaatt tggattgaaa gacggcaaaa taggaactgc tgtccaaggt
     1561 gaagttcccg gaactataat taatcaattt tcaatggatg agcataacaa cacctttaga
     1621 attgcaacaa ccacaggtga tatgtggatg aatacatcca aaaacaatgt atatattttg
     1681 gatgaagacc taaaagtttt gggaaaattg gaaggtttgg ctgaaggtga aagaatttac
     1741 agcacacgct tcgccggaga cagaatgtac atggttacat tcaggcaggt tgaccccttg
     1801 tttgtaattg acacatcaaa cccaagagct cccgagttaa agggaatgct taaaataccg
     1861 ggatacagca catatcttca tatggttgat gaaaatcaca ttttaggatt tgggtatgat
     1921 actgaagtaa atcaatgggg cggaaccgta aacggcggct taaaaatttc aatgtttgat
     1981 gtttcaaatg tgaataaacc aaaagaaaca tttacggaaa taatagggaa gtcgggaaca
     2041 tattcagagc tcttgtacaa tcacaaggca ttgatgtttt cattaaataa gggattaatg
     2101 gcatttcccg taaacagaac agcagaaaat tataaaagcg actttagcgg agcatatgta
     2161 tataatgttt ccaatgacag cataagttta aaaaatacaa taactcacaa agaatctgaa
     2221 tttgattctt acggagataa tatcaaaagg ataatttata tcggtgatta tttgtacaca
     2281 ttttctgaaa ataaaatgca aattcacggc atcaatacaa ataagaaggt taatgagtta
     2341 ataattaaat aaataaaggt gccaggcacc tttttgggtg cctggcacca atatacctat
     2401 gggagagttt atgaaaaagt ctaatttttt tgcatttata tcaagaatga agtatattga
     2461 ccgttg
//
LOCUS       DAOSOW010000291         2448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_42943_length_2448_cov_3.744254, whole genome shotgun sequence.
ACCESSION   DAOSOW010000291 DAOSOW010000000
VERSION     DAOSOW010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42943_length_2448_cov_3.744254"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PLD67_07455"
     CDS             <1..550
                     /locus_tag="PLD67_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT22395.1"
                     /translation="EGGGMDMPIEEPMPEPVPEEPEEPGEKENVFEKGEEPLTITSLN
                     NLVKEADQANRIMTVLLGGIAAVSLLVGGIGIMNIMLVAVTERTSEIGVRRALGAKKE
                     DLLIQFLLEAVYVSIIGCVAGIVLGAWAISIVAKYGLSAVVSLDSIRIAVFVALTSGL
                     LFGVYPAINASNLPPVEALKRQ"
     gene            695..1078
                     /locus_tag="PLD67_07460"
     CDS             695..1078
                     /locus_tag="PLD67_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOT22396.1"
                     /translation="MELNLSVGLKYKEEKTVTINDTAKVFGSGAAEVFATPMMIGLME
                     AASMNAVKNFLPRGCSTVGTLVNVKHMSATRVGKKVWAEAELIEIDRRRLVFNVTAYD
                     EDKKIGEGTHERFVIDDEKFMSNLK"
     gene            1108..1569
                     /gene="nrdR"
                     /locus_tag="PLD67_07465"
     CDS             1108..1569
                     /gene="nrdR"
                     /locus_tag="PLD67_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOT22397.1"
                     /translation="MRCPKCNYTESKVIDTRPTDDGFKIRRRRECIECGHRFTTYEKI
                     EETQIVVIKRDGTRQGYNRDKIINGLIRACDKRSVSLEQIEKIADKVEKQLYNTFQNE
                     VSTEIIGELVMNELQNVDDVAYVRFASVYRKFKDIDTFMDELTKILNERKK"
     gene            1579..2211
                     /locus_tag="PLD67_07470"
     CDS             1579..2211
                     /locus_tag="PLD67_07470"
                     /inference="COORDINATES: protein motif:HMM:NF025391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4241 domain-containing protein"
                     /protein_id="HOT22398.1"
                     /translation="MYINLDVLNITNGNTHIIDSVFGRMNIEKIEAGKLKLLTGRIIT
                     TDPVLLYDDECFSEHVKPGLYTVNIYVGKCGKRKKQTVAAELRINENVPVKWEMALLE
                     GESAKGFSSDEFMGYEVENGLGCFMDEKVMEMLDIMSEEELNDYETKVKDAVRQSDSS
                     CADIIMDEEKGLNIIVFASGWNEGTFPTYYGFDKNNKLSRLVTDFMVIEK"
BASE COUNT          883 a          323 c          540 g          702 t
ORIGIN      
        1 agaaggcgga ggaatggata tgccaataga ggagccaatg cctgaacctg ttcctgaaga
       61 accggaagaa ccgggtgaaa aagaaaatgt ctttgaaaag ggtgaagagc cgcttacaat
      121 tactagttta aataatttag taaaggaagc tgaccaggca aacagaataa tgacagtact
      181 acttggaggt attgcggctg tatcgcttct ggtaggcggt ataggaatta tgaacataat
      241 gcttgtggca gtgacggaaa gaacaagcga aataggagta agaagagctt taggagctaa
      301 gaaggaagat ttattaattc agtttttatt ggaagctgtc tatgtaagca taatcggatg
      361 tgttgcggga attgttcttg gtgcttgggc aataagtatt gtggcaaaat acggactttc
      421 tgcagtagta agtcttgatt cgatcagaat agcagtattt gttgcactga cttccggact
      481 tttgttcggt gtatatcctg caataaatgc ttcaaattta ccgccggttg aagcattgaa
      541 aagacagtaa gaacgtttgg tgacatgctt ttctcggtgc caggcaccat ggcggtgcct
      601 ggcaccaatg tctatgaggt tttaatttca aaacctcttt tattttatat tattttattg
      661 tataatacgg taagaacagg tattggaggt aataatggaa ttaaatctat cagttgggtt
      721 aaaatacaag gaagaaaaaa ctgttacaat aaatgatacc gcaaaagttt tcggcagcgg
      781 tgctgctgaa gtttttgcaa cgcctatgat gattggtcta atggaagcag cttccatgaa
      841 tgccgtaaaa aattttttgc caaggggctg ttcaactgtt ggaaccttag ttaatgtaaa
      901 acacatgagt gctacaagag tcggcaaaaa agtgtgggca gaagcagagc ttattgaaat
      961 agacagaagg agattggttt ttaatgtgac agcttatgat gaagacaaaa aaataggaga
     1021 aggaacccac gaaagatttg ttattgatga tgaaaaattc atgagtaatt tgaaataatt
     1081 tgtcattgct atgaaaagga gtaatttatg agatgtccaa aatgtaatta tacagagagc
     1141 aaggttattg atacaagacc taccgatgat gggtttaaaa taagacgtcg aagggaatgc
     1201 atagaatgcg gacatcgttt tactacatac gaaaaaatag aagaaactca aattgtagta
     1261 attaaaagag atggaacaag gcagggttat aacagagata aaataataaa cggtcttatc
     1321 cgtgcttgcg ataaacgctc cgtaagtctt gagcaaattg aaaaaattgc ggataaggtt
     1381 gaaaaacaat tgtacaatac atttcaaaat gaagtttcaa ccgaaattat cggggaattg
     1441 gtcatgaatg aacttcaaaa tgtggatgat gtggcatatg taagatttgc atcagtttac
     1501 agaaaattta aagacattga tactttcatg gatgaattaa caaaaatttt aaatgaaaga
     1561 aaaaagtaag gtgataagat gtatataaac ttagatgttc ttaatattac aaacggcaat
     1621 acacatatca tagactccgt atttggcagg atgaatattg aaaaaattga agcagggaaa
     1681 cttaaattgt tgaccggcag aataataacc acagacccgg tattattgta tgatgatgaa
     1741 tgtttttcgg agcatgttaa gccgggatta tatactgtca atatatatgt tggaaaatgc
     1801 ggaaaaagaa aaaaacaaac tgttgccgcc gagctgagaa tcaatgaaaa tgtgcctgtt
     1861 aaatgggaaa tggctttatt ggaaggtgaa tccgcaaaag ggttcagcag cgacgaattt
     1921 atgggatatg aagttgaaaa cgggctcgga tgtttcatgg atgaaaaggt catggaaatg
     1981 cttgatatta tgagcgaaga agaattaaat gattatgaaa caaaagtaaa ggatgcggta
     2041 cgacaaagtg acagctcatg tgcagatata attatggatg aagaaaaagg tctaaacata
     2101 attgtttttg ccagcggctg gaatgaagga acattcccta cttattacgg ttttgacaag
     2161 aataataaac tatcccggct tgtaactgat tttatggtaa tagaaaagta gtactgccta
     2221 accgatactt ctccctgcaa gccctatttt tcagagtctt tttttgcgtt tcttaaataa
     2281 ttattaaaaa cacatatata aacatataca ttcttgaaat attaattaat tggtgataaa
     2341 ataaataatc tattaaatat gacgaaggaa tactatgaat aagaaaatca acaaatatga
     2401 tattggcctt atagtatcta taataattat tagtcttttc tttatttt
//
LOCUS       DAOSOW010000292         2448 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_42952_length_2448_cov_2.950272, whole genome shotgun sequence.
ACCESSION   DAOSOW010000292 DAOSOW010000000
VERSION     DAOSOW010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42952_length_2448_cov_2.950272"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1450
                     /locus_tag="PLD67_07475"
     CDS             23..1450
                     /locus_tag="PLD67_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003478598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOT22399.1"
                     /translation="MRVLEGLKPEKVFYYFEEISKIPRCSGKEKQISDYLYNFAKSRN
                     LEVIQDEHLNVIIKKPASRGYENAPVVMLQAHMDMVCEKNEGVEHDFDKDPIKLRIED
                     GYIYAADTTLGADNGIGVAFALSVLDSELEHPSLEVLITTDEEKGMTGADKLDGSLFK
                     SKYLLNLDSEEEGVFTAGCAGGSEIDFKIPVKYQNTKGRAYKISVKGLSGGHSGVDIH
                     KEKGNANKILGRILYDLIDDVELASIEGGSKTNAIPREANAIVTINSDKVPEKIAKWN
                     KILKNEYAFTDPSIRVALTDLNEEVFPLEKETYKKTVALINLIPVGVLSRSTAIDLVI
                     SSNNLGVIRTDEKYITLFNHPRSSVETLLTEDFIPAMKQLASILDVEYVLGSYYPGWE
                     YAKESNIRDICIEAYKDMFNKEPLVGAIHAGLECGLLMDKIPGLDAISMGPDAYDAHS
                     PNEHVSISSVENIYNLLCEILKRIK"
     gene            1717..>2448
                     /locus_tag="PLD67_07480"
     CDS             1717..>2448
                     /locus_tag="PLD67_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT22400.1"
                     /translation="MDSKLALYIVKRLTLAVITIFLIITITFFVMHEVPGGPFLSEKA
                     PSAAVTAALEAKYGLDKPKSEQYVTYLKGIMKGDFGPSIKQRGREVTAVIMQGFKVSF
                     KLGGTAAVIALCIGTVLGSVAAIFHNKTIDKIIMVVTTGLVAMPSFIIATLLLLVFCV
                     WMGIFPANGNEPGGLILPIISLSLYPMSYTTRLTRSSMLDVLSQDYIRTARAKGVKPA
                     KVIFKHALRNAVTPVVTYAGPMFAYI"
BASE COUNT          847 a          347 c          519 g          735 t
ORIGIN      
        1 atcaaactat atggaggttt acatgagagt tttggaaggt ttaaaaccgg aaaaggtatt
       61 ttattatttt gaggaaatat ctaaaatacc tcgttgttca ggaaaagaaa agcaaatttc
      121 agattatctg tacaattttg ctaaatcaag aaatttagaa gtgatacaag atgagcacct
      181 gaatgtaatc attaaaaagc ctgcttcaag aggatatgaa aatgcacctg tagtaatgct
      241 tcaagcccac atggacatgg tgtgtgagaa aaatgaaggt gtcgagcatg attttgacaa
      301 agacccgatt aaattaagaa tagaagatgg atatatttat gctgctgaca caacccttgg
      361 agccgataac ggaataggag tggcttttgc attgtctgtg cttgattccg agctggagca
      421 tccttccctt gaagtattaa taacaacaga tgaagaaaag ggaatgacag gagctgataa
      481 actcgacggg agcttgttta aaagcaagta tttattgaat ttagacagcg aagaagaagg
      541 agtgtttact gccggatgtg caggaggaag tgaaattgat tttaaaatac ctgttaaata
      601 tcaaaacaca aagggcaggg catataaaat aagtgttaaa ggattgtccg gaggtcactc
      661 gggagtagat attcataaag aaaaagggaa tgcaaataaa attcttggaa gaattcttta
      721 tgatttaata gatgatgttg aattagcaag cattgagggt ggctcaaaaa caaatgcaat
      781 accgagagaa gcaaatgcaa tagtaacgat aaattcagac aaggtacctg aaaaaatagc
      841 taaatggaac aaaatactca aaaatgagta tgcatttacc gatccgtcaa taagggtagc
      901 attaacggat ttaaatgaag aagtatttcc tttggagaaa gagacatata agaaaacagt
      961 tgctttaata aatttgattc ccgttggtgt actatcaaga agcacagcta ttgatttggt
     1021 tataagttcc aataacttgg gtgtcataag aactgatgaa aaatatataa cactttttaa
     1081 tcaccctcga agcagtgtgg aaacattgct taccgaagac tttattcctg ccatgaaaca
     1141 gttagcatca atattggatg tagagtatgt attgggaagt tattatccgg gctgggaata
     1201 tgcaaaggaa tctaatatta gagatatttg cattgaggct tataaggata tgtttaataa
     1261 ggagcccctt gtaggtgcaa tacatgcagg tctggaatgc ggacttctga tggataaaat
     1321 tccgggactt gatgcaatat caatgggacc tgatgcatat gatgcgcatt ctcctaacga
     1381 gcatgtaagc ataagctctg tagaaaatat atataattta ttatgtgaaa tattaaagag
     1441 aataaaatag ttataacaag gaatatgttg tcgttttttt atattgataa catattcttt
     1501 taatataatt ttttgtatat tagaattact aaacttatgc atattatggt tttaatatta
     1561 ttttcctggc atataaatac aattcaggca taaaaataaa tatattgata taggaaaaat
     1621 cttatggtat aattatttgt gttttaaata gaattgagat gactctgagg gagctttatt
     1681 agcatcctca ttatatttaa aatgttggag gtaataatgg attcaaaatt agcgttatat
     1741 attgttaaga gactgacctt ggcagtgata acaatatttt taataatcac cattactttt
     1801 ttcgtaatgc atgaagttcc gggaggtcct ttcttgagcg agaaagcacc aagtgcggct
     1861 gttacggcgg cgctggaagc aaaatacgga ttggacaaac ctaagtctga acagtatgta
     1921 acatacttaa agggtattat gaaaggtgac ttcggtcctt cgattaaaca aagaggaaga
     1981 gaagtaacag cagttattat gcagggcttt aaagtaagtt ttaaattagg aggaactgct
     2041 gcggtgatag ctctttgtat aggaactgta ttaggctcag ttgcggctat ctttcataat
     2101 aagactatag ataaaataat tatggttgtt acaaccggcc tggtcgccat gccgtcattt
     2161 attatagcaa cattgctttt actggtcttt tgtgtgtgga tgggaatttt tccggccaat
     2221 gggaatgagc ccggcggact aatacttcct attatatctc tcagccttta tcccatgtca
     2281 tacactacca gacttacaag gtccagtatg cttgatgtat taagtcagga ttatataaga
     2341 acggcaaggg caaaaggtgt gaaaccggct aaggtaatat ttaaacacgc tctccgaaat
     2401 gccgtaacac ctgttgtaac atatgccggt ccaatgtttg catatatt
//
LOCUS       DAOSOW010000293         2433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_43254_length_2433_cov_4.038688, whole genome shotgun sequence.
ACCESSION   DAOSOW010000293 DAOSOW010000000
VERSION     DAOSOW010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43254_length_2433_cov_4.038688"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1453)
                     /locus_tag="PLD67_07485"
     CDS             complement(<1..1453)
                     /locus_tag="PLD67_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOT22401.1"
                     /translation="MEQDNKRGQWASNFGFLMAAIGSAIGLGNIWGFPYKMGANGGFI
                     FLLIYLTLVAFVGFATMLGELTLGRKYGMGVIGTYKKISEKYAWIGWLGWLSGFLILA
                     FYSVLGGFTVRYFFGFLMELFSSGAGFGGMDSGAFFGNFIINAPMNILYHAIFMGATV
                     IIVMGGIQGGIEKFTAVAMPALFVMIIIVVIRSITLEGSFVGLEFMFKPDFETLKEIG
                     LMRVLKTAAGQMFFSLSLGMGCMITYGSYLSKNENLEKNAMIIPIADTTFATLSALAV
                     LPAVGAFMYQGVEGVSFGAGPGLLFVTLHRVFSVGMGGFMGNFFGAFFYFLVFIAAVT
                     SSISLLEVCTAYFIDKRVEKGLDPDRKKITLIMGVIIFLLGFPVAMDGLGSGVAGGAM
                     IDIPAVVFGMKEVRVAFDCWLDFYDMISEGVFMPLGAMLMSIIIGWKLKTVLIKEEVE
                     ETPGYKMRSYNFWDVCFKYLVPAAMFFVLLGQLD"
     gene            complement(1863..>2433)
                     /locus_tag="PLD67_07490"
     CDS             complement(1863..>2433)
                     /locus_tag="PLD67_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HOT22402.1"
                     /translation="TIPESTAHVYQLDIYVNDLAKKKGAGKIYDIENKLGLNLIGDGM
                     GDIISGFIGGPAGTNYGENISAMAITKNFSVHVLILASILTMIISCFTPLINIIYSIP
                     TAVIGGLSIYLFGVIGAQGITIMLDRKVDMFSAKNLAVIAVILIVGLGGSFGFAGGMI
                     PMFGIEVPAIASAAVVGILLNLMLSIGEK"
BASE COUNT          834 a          526 c          337 g          736 t
ORIGIN      
        1 catccagctg tccaagcaat acgaaaaaca ttgcagccgg aacaagatat ttaaagcaaa
       61 catcccagaa attatatgat ctcatcttat agccgggagt ttcttcaacc tcttccttaa
      121 tgagaactgt cttgagtttc cagccgatta taattgacat taacattgca ccaagaggca
      181 tgaatacacc ttcagaaatc atgtcataga agtcgagcca gcagtcaaat gcaactctaa
      241 cctctttcat gccgaataca acagcaggaa tatcaatcat tgcacctcct gctacgcctg
      301 agccaagacc atccattgct acggggaatc ctaataagaa tattataact cccataataa
      361 gggtaatttt cttacgatca ggatctagac ccttctcaac tcttttgtct ataaagtatg
      421 ccgtacaaac ttccaataga gaaatagacg aagtaacagc agctataaat actaagaaat
      481 agaaaaatgc accaaagaaa ttacccatga atccgcccat acctacactg aatactctat
      541 gcagagtaac gaataatagt ccgggacctg ctccgaaaga aacaccttca accccttgat
      601 acataaatgc acctactgcg ggtaatacag ctaaagctga tagtgttgca aatgttgtgt
      661 ctgctatagg aataatcatg gcatttttct ctaagttttc atttttgctg aggtaggagc
      721 cgtaagtaat catgcatccc ataccgagag acagtgagaa gaacatctgt ccggctgcgg
      781 tttttagaac cctcataagg cctatttcct ttaatgtctc aaagtcaggt ttgaacataa
      841 attctagacc tacaaaagaa ccttccaaag taatacttct gataactacg attataatca
      901 tgacaaacaa tgccggcata gctactgcag taaatttttc aataccgcct tgaattccac
      961 ccattactat aattactgta gcgcccataa aaattgcatg atataaaata ttcattggag
     1021 cattgataat gaaattacca aaaaatgcac cgctgtccat tcctccaaaa ccggcacctg
     1081 aactaaacag ttccattaag aacccaaaga aataacgtac agtaaaaccg cctaatacgc
     1141 tataaaatgc taaaattaaa aaacctgaaa gccagcccag ccatcctatc caagcatact
     1201 tttcgctgat tttcttataa gttccgataa ctcccatacc atacttacgg cctaatgtaa
     1261 gctccccaag catagttgca aagcctacaa aagctaccaa agtcaaataa attaatagaa
     1321 atataaatcc accgtttgca cccattttat atgggaatcc ccaaatatta cccaagccta
     1381 tagctgagcc tatagctgcc atcaagaaac caaaattact ggcccactga cctctcttat
     1441 tatcttgttc cattttacac catcctttct tcttttaaga tacgaatgca ataccaataa
     1501 ttacttatta ttaactatat ctaaataacc tcctatcttt tttaagtttt ttttaatttc
     1561 acccttataa aataaggttt tataacgtat gcatttaata atcttatgta taaaaatgaa
     1621 tgcataaaat actattatgt ccctgcaaac gtatcctttc atgaattata ctatatagca
     1681 tttggtttgt ccagcaaaaa ctatgttata tttaaaacaa tcaatgaata aaataataat
     1741 tcataatgtt tatattattc atttgtataa aatactgcat atgaatatat attgatatac
     1801 actaaataaa gattcctcta cggtcagagg aatcttattt tttgtatatt attttttgta
     1861 tactattttt cacctattga cagcataaga ttaagaagta ttccgacgac ggctgctgaa
     1921 gctatggcag gcacttcaat accaaacatc ggaatcatac cgcctgcaaa tccaaagctg
     1981 ccgccaagcc ctactattaa aattacagct atcaccgcta aatttttagc gctgaacata
     2041 tcaacctttc tatccagcat aatggttata ccctgtgctc ctataacacc gaataaataa
     2101 atcgataatc ctccgattac tgctgtagga attgaatata ttatgtttat taaaggtgta
     2161 aagcaggata taatcatagt taatattgat gctaatatga gaacatgtac ggagaaattt
     2221 ttggttattg ccattgcact tatattttct ccatagtttg tacctgcagg acctccaata
     2281 aatcctgaaa taatgtcccc cattccatcg cctataagat ttaatcctaa tttattttca
     2341 atatcatata ttttgccggc accttttttc ttggctaaat catttacata tatatctaac
     2401 tgataaacat gtgccgtcga ttccggtatt gtt
//
LOCUS       DAOSOW010000294         2429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_43344_length_2429_cov_4.080876, whole genome shotgun sequence.
ACCESSION   DAOSOW010000294 DAOSOW010000000
VERSION     DAOSOW010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43344_length_2429_cov_4.080876"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PLD67_07495"
     CDS             complement(<1..298)
                     /locus_tag="PLD67_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HOT22403.1"
                     /translation="MNKKKLFAIIVCIIIALVATYIGGAQRIIGAPMLGLLIGMAVVN
                     MIPSVDKDFKSGTSFAGKKFLNLGIILTGATLNFNQILGYGARAMPLLLFNICVA"
     gene            complement(449..1399)
                     /locus_tag="PLD67_07500"
     CDS             complement(449..1399)
                     /locus_tag="PLD67_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HOT22404.1"
                     /translation="MANLKVTPAEEKTVKALGFLRNKGTDNFSARIITVNGKITAAQQ
                     KIIGEAAEKFGNGDVTFTSRLTIEVPGIPYEKITEFQDYIGKEGLVTGGTGSKVRPVV
                     ACKGTVCHHGLIDTFDVSEDIHNIFFEGYAPVKLPHKFKIGVGGCPNNCMKPSLNDLG
                     VIGQMVPNYDEELCMGCKKCSVEEACPIGAAKVVDGTLVIGDDCNNCGKCIGTCNFDA
                     IPDGDKGYKVFIGGMWGKRVNPGVPINKIFKTKEEVFEIIEKMILLYREQGQTGERVA
                     QTVNRIGFENVEAQLLSDEIFERKQEILEAELHMTGGATC"
     gene            complement(1432..>2429)
                     /locus_tag="PLD67_07505"
     CDS             complement(1432..>2429)
                     /locus_tag="PLD67_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT22405.1"
                     /translation="TETGLEAILGDGKVEKIKTNNRTMKADAVVLSIGIRPNTAFLNG
                     SGIELAPNRAIKVNEKLETNIKDIYAVGDCAFVTNIITGKGAYAPMGSTANIEGRILA
                     QNINGNNLNYKGAIGTAVVKLPGINAGKTGLTEDAAKSEGYNVETVTTVMEDKAKYYP
                     GASVFIIKMIADKETKKLLGVQVVGEGNIDKIVDMAVTAIGLDAKLDDIQNLDYAYAP
                     PFSTAIHPFAATVNVLLNKINGNLVSVTPREYKEGKADGYKIIDSSIVPKITGAPYVD
                     ISKAHEELPEFDKDEKLLLVCDKGKRSYMLQQRLKDFGYTNTLVLEAGTMFNELK"
BASE COUNT          698 a          506 c          339 g          886 t
ORIGIN      
        1 aagctacaca tatgttaaat aataataaag gcatagctct tgccccatag cctaatattt
       61 gattaaaatt aagtgttgca ccggttaata taatacctaa attcaaaaat ttctttcctg
      121 caaagcttgt acctgattta aaatctttgt ctacagacgg aatcatgttt actacagcca
      181 taccaatcaa tagtcctaac attggagcac cgattattct ttgagctcca cctatatatg
      241 ttgcaacaag agctataata atgcagacaa ttattgcaaa aagtttcttc ttattcatga
      301 cattttctcc ttctaattac ataataatac caagcgttca aaaacaccat acttaaaatt
      361 taatctttct ccggtttgtt aattattttt ttaacgaagt atgagaaaat tatctccgga
      421 agaaatattc ttccggatat agttttcttt agcaggtagc tccgcctgtc atatgaagtt
      481 cagcttctaa tatttcttgt tttctttcaa atatttcatc agataacaat tgagcttcaa
      541 cattttcaaa tccgattctg tttactgttt gagcaactct ttcacctgtt tgtccttgct
      601 ccctgtaaag gagtatcatt ttttcaataa tttcgaatac ttcttccttt gttttaaata
      661 ttttgtttat gggaactccg ggatttactc ttttacccca cattccgccg ataaatactt
      721 tatatccttt atcaccatcc ggaatggcat cgaaattaca agttcctata cattttccgc
      781 agttgttgca atcgtcaccg attactaatg taccatcaac aacttttgca gcaccaattg
      841 gacatgcttc ttctacagag catttcttgc aacccatgca aagttcttca tcatagttag
      901 gcaccatctg cccgattacg ccgaggtcat ttaatgaagg cttcatgcag ttatttggac
      961 atccgcctac acctattttg aatttatgcg gtaattttac cggtgcataa ccttcaaaaa
     1021 atatattgtg tatatcttct gatacatcaa aagtatcaat taatccatga tgacaaactg
     1081 tacccttaca agctactacc ggacgaacct tagagcctgt acctcctgta actaatcctt
     1141 ctttaccgat ataatcttgg aattcagtga ttttctcata aggtatgcct ggtacttcaa
     1201 ttgtcaacct gctggtaaat gttacatctc cgtttccaaa tttctctgca gcttctccta
     1261 ttattttttg ttgagctgct gtaatctttc cgttaacagt aataattcga gcagaaaaat
     1321 tgtctgtacc cttatttctc aaaaaaccaa gagctttaac agtcttttct tctgcaggtg
     1381 taacctttaa atttgccata attttattct cctcttctaa atctatatta tttattttaa
     1441 ttcattaaac atagtaccgg cttcaagtac taaggtattt gtatatccga aatcttttaa
     1501 tctctgttgc agcatatagg atctcttgcc tttatcacat actaacaata gtttttcatc
     1561 tttatcaaat tcaggcaatt cttcatgtgc ctttgaaata tctacataag gagcacctgt
     1621 aattttagga actattgatg aatctataat cttatatccg tcagccttgc cctctttata
     1681 ttctcttggt gtaacagaaa ccaaattacc gtttatttta tttaataaaa catttacggt
     1741 agccgcaaaa ggatgtattg ctgttgaaaa tgggggagca taagcatagt cgagattttg
     1801 gatatcgtct aatttagcat ctaatccgat tgccgtaaca gccatatcta ctatcttgtc
     1861 aatattgcct tcaccgacaa cttgtacacc caacagtttc tttgtttctt tatctgcaat
     1921 catctttatg ataaaaactg aagcaccggg ataatatttt gctttgtctt ccattacggt
     1981 agtaacagtt tctacattat aaccctctga cttagcagcg tcttccgtca aaccggtttt
     2041 tccggcatta attcccggaa gtttaacaac tgctgttcca atagcacctt tgtaatttaa
     2101 attgtttcca tttatgtttt gtgctaatat tcttccttca atattggcag tagagcccat
     2161 gggagcataa gcacctttac cggtaataat gtttgtaaca aaagcacaat cacctactgc
     2221 ataaatatct ttgatattgg tttcaagttt ttcattaacc ttgatagctc tatttggtgc
     2281 taattcaata ccgctaccgt ttaaaaaagc tgtattaggc cttataccta ttgataaaac
     2341 tactgcatct gctttcatgg tacgattgtt tgttttgatt ttttcaactt ttccatcgcc
     2401 caatatagct tctaaaccgg tttcggtaa
//
LOCUS       DAOSOW010000295         2423 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_43501_length_2423_cov_2.880490, whole genome shotgun sequence.
ACCESSION   DAOSOW010000295 DAOSOW010000000
VERSION     DAOSOW010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43501_length_2423_cov_2.880490"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLD67_07510"
     CDS             complement(<1..196)
                     /locus_tag="PLD67_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019035000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT22406.1"
                     /translation="MSDNLLSLKNVSKEYSGNRVLKDINIELKKGQIHALIGENGAGK
                     STLMNILFGMSVIHTTGGFEG"
     gene            complement(316..1539)
                     /locus_tag="PLD67_07515"
     CDS             complement(316..1539)
                     /locus_tag="PLD67_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HOT22407.1"
                     /translation="MKRLLSLLLALMMVLSLAACTQPAQTPPAEEKPAEEKPAEEKPA
                     EEKPAEEEPFEGIIAIVTNTLSQNEEEYRSAELMVQTYGEDKIMHVLWPDNFMTEQEQ
                     MISTIAKIGSNPDVKGLIINQAVPGTNAAVDKLLETRDDIFISYCTPQENPPDVAERA
                     DLILNPDELKMGDAIPIQAQKLGAKTLVHYSFPRHMSIVMLAARRQLMIDKCKEIGLE
                     FYDATAPDPTSDVGVPGAQQFILEDVPKLVDRFGKDTAFFSTNCAMQTPLIKATSDAG
                     AIYPQPCCPSPYHGYPSALGIESTGYSVDAMANVISETAKALKAKGVLGRFSTWPVPV
                     AMMNTVASTEYIIEWINGNVGDELDVEVLEEKMAEYAKLAVTTSSYTEEGLEIPHFRL
                     IMMDFLTYGEEHILD"
     gene            complement(1826..2134)
                     /locus_tag="PLD67_07520"
     CDS             complement(1826..2134)
                     /locus_tag="PLD67_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22408.1"
                     /translation="MSNIFRSDNEINKIKDSKEFKDAEAKANELAKNKQFQEFKKKYE
                     GKSEEDILRDARNMSKKLKQQYGEEEYNKKIEDLKNFEKFLTPEQKRKMRKFLENLED
                     "
BASE COUNT          665 a          462 c          380 g          916 t
ORIGIN      
        1 ttccttcaaa tccgccggtt gtatgaatta ccgacattcc aaatagaata ttcatcaatg
       61 tggattttcc tgcgccgttt tcgcctataa gcgcatgaat ttgacctttt tttaattcaa
      121 tatttatatc cttcaatact ctatttcctg aatattcttt tgaaacattt tttaagctta
      181 gaaggttatc actcattact acctcctgtt tattgacata actaattagc aaaggaatga
      241 atcagaagtt cctaataaga aattctgaaa cattccttaa tgtttacatt atttaattat
      301 agtactatat atcgattaat caagaatatg ttcttctccg taagttaaga aatccatcat
      361 gattaatctg aaatgaggaa tttctaatcc ttcttctgta tatgaacttg tagtaacagc
      421 taattttgca tattcagcca ttttttcttc aagtacttct acatcaagct catcgcctac
      481 attaccgtta atccattcaa tgatatattc tgtagaagct accgtattca tcatagcaac
      541 aggtaccggc catgttgaga atcttcctaa tactcctttt gccttcaatg ctttggctgt
      601 ttcgcttatt acgtttgcca tagcgtcaac ggaataacct gtagattcaa tacctaatgc
      661 agatggatat ccgtggtatg gtgacgggca gcaaggttgc ggataaattg ctcctgcatc
      721 gctcgttgct ttaataagcg gagtctgcat tgcacagttt gttgagaaga atgctgtgtc
      781 ttttccaaat ctatcaacta acttcggcac gtcttcaaga atgaactgct gtgctccagg
      841 tactccaacg tcacttgtcg ggtcaggtgc tgttgcatca tagaattcaa gtccgatttc
      901 tttacattta tcaatcatta attgacgtct tgcagcaagc attacgattg acatatgtct
      961 cgggaatgaa tagtgtacca atgttttcgc acccaatttc tgtgcttgta taggaatagc
     1021 atctcccatc ttcaattcgt cagggttaag tattaagtct gctctctcag caacgtcagg
     1081 tggattttct tgtggtgtac agtaagaaat aaatatgtca tcacgagttt ccaagagttt
     1141 atctactgct gcgtttgttc ccggaacagc ctgatttata atcaatccct taacatccgg
     1201 gttagagcct atttttgcaa ttgtagaaat catttgttcc tgctcggtca tgaaattatc
     1261 aggccataat acgtgcatga ttttgtcttc gccatatgtt tgaaccataa gttcagctga
     1321 acgatattct tcttcgtttt gagaaagagt gttcgtaaca atggctatta tgccttcaaa
     1381 cggttcttct tctgcaggct tttcttctgc aggtttttct tctgccggtt tttcttctgc
     1441 cggtttttct tctgccggcg gagtttgtgc aggttgtgta caagctgcta acgataaaac
     1501 catcatcaga gccaacaata aacttaataa tcttttcatt acttgtccct cctaatttat
     1561 acacataaaa atatgtattc atattataat tgaatatact tctcatgtca aactattttt
     1621 gaaaacgtta tatacttctt acttactaaa ttatggtttt gtgtgtgttg tgcgtacatt
     1681 tcaccgcatt ttttgtatgt cagcaatatt tataaaatgt taataattgt aaactttatt
     1741 cttgcgcata ttacaagcgg agcttttgat tatatctatg gaatataatt atgggtttga
     1801 atggctaaaa gggagctttt gctccctaat cttccaagtt ttctaaaaac tttctcattt
     1861 ttcttttctg ctccggtgtc aggaatttct cgaaattttt taaatcttca atttttttgt
     1921 tatattcctc ttcaccatat tgctgtttta actttttgct catatttctt gcatcccgta
     1981 aaatatcttc ttccgatttc ccttcatact tttttttaaa ttcttgaaat tgtttatttt
     2041 ttgctaattc atttgcttta gcttcagcat ccttaaattc tttgctatct ttgattttat
     2101 taatttcatt gtcacttcta aatatatttg acattcatct acctcctata tgttatataa
     2161 tatgccgccg ctgtcattta attacaacag tgtttataca attacagttt gctctgtaac
     2221 ataatcatgc aatcggaata tgatatatgg agatatttat aagaaggtga tattattccg
     2281 gttcaaatat tattatatat tttagcggca aaatatattt ttgacggcaa tcaattaaac
     2341 ggaatcgttt ccaagctttc aggaacaact aaaacgatgg cctcgggaat acttaaagac
     2401 ccggctaaga tggaaaattt aat
//
LOCUS       DAOSOW010000296         2420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_43564_length_2420_cov_4.446512, whole genome shotgun sequence.
ACCESSION   DAOSOW010000296 DAOSOW010000000
VERSION     DAOSOW010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43564_length_2420_cov_4.446512"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..521)
                     /locus_tag="PLD67_07525"
     CDS             complement(138..521)
                     /locus_tag="PLD67_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOT22409.1"
                     /translation="MMNSVIHTNNAPAAVGPYSQGIKAGNTIYVSGQLAINPATGELY
                     KGGDIKEETRMSLMNVKAILEEAGATLKDVVKCTVFIKDMNQFGLINEAYAEVFQDTK
                     PARVCVEVARLPKDGNVEIDAIAVV"
     gene            complement(528..896)
                     /gene="rsfS"
                     /locus_tag="PLD67_07530"
     CDS             complement(528..896)
                     /gene="rsfS"
                     /locus_tag="PLD67_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOT22410.1"
                     /translation="MIEVQKNIETILKACDNKKAYNFVVLNVSKTSSITDYFIICSGN
                     NEKQTVAIAEEVLEKGAKEGIDFYYKEGFETGRWILLETDDIIVHIFHKDEREIYDLE
                     SLWYDNNSLDVEQYGIKNWK"
     gene            complement(893..1393)
                     /gene="yqeK"
                     /locus_tag="PLD67_07535"
     CDS             complement(893..1393)
                     /gene="yqeK"
                     /locus_tag="PLD67_07535"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HOT22411.1"
                     /translation="MHIEEMKKELKIELGEKLYRHSIGTMEEAEKLASVYGCDVSKAI
                     IAGLLHDCGKRICSDNLKHAKKSAELAGVKYGVKDNDILNAIMFHTTGKVNMSLLEKI
                     IYIADKIEPNRNYEGVEEIRNKAYTRLDEAIIKSLENTIEYVKNRNLVLDMESVNTLN
                     YLKEGK"
     gene            complement(1428..2045)
                     /gene="nadD"
                     /locus_tag="PLD67_07540"
     CDS             complement(1428..2045)
                     /gene="nadD"
                     /locus_tag="PLD67_07540"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOT22412.1"
                     /translation="MKKKVGIMGGSFDPIHMGHLVAANEALNIYKLDEIIFIPTGDPP
                     HKDYPNAESFHRLLMVNLAVLTNDKFSVSDIEIKNPSKSYTLSTLRELHKLYKNTEFY
                     FITGTDAVLNLPSWHQPEEVLKLCRFIAATRPGISIEEVNTKINEIKRMFGGNIEILQ
                     VPMLQISSSDIRNRIKMGVSIKYLLPDSVEQYIIKNGLYKKGQEK"
     gene            complement(2042..>2420)
                     /locus_tag="PLD67_07545"
     CDS             complement(2042..>2420)
                     /locus_tag="PLD67_07545"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HOT22413.1"
                     /translation="AGLGDSFTQAILAPLFISMSVMLCLDGSFYLAFIPVIILSVFIL
                     FISYNGFMNGYFQGRESMLQRIKDVKQSKIKLYFPYIFSGILGFSISKLLFNNIRPYE
                     DIFTLSIILFAAVIMFIRKRGEQ"
BASE COUNT          711 a          514 c          321 g          874 t
ORIGIN      
        1 acacaagtac ggggacattc cttcatggcg ttaccccaac ggctgactca ccatgaaggt
       61 gtgtcccccc aatttgggga cattcctcct atccctcaga gactatccat aaaaaaaaga
      121 ggtgtgtccc cacaccttta aacaactgct attgcatcaa tttctacatt tccgtcctta
      181 ggaagtctag ccacttctac acaaactctt gccggttttg tatcttggaa tacttcagca
      241 taagcttcat taattagtcc gaattgattc atatccttga taaataccgt gcatttaaca
      301 acatccttca atgttgctcc tgcttcttcc aaaatagctt tcacattcat taaggacatt
      361 cttgtttctt ctttaatgtc accgcccttg tacagctccc ctgttgccgg atttattgca
      421 agctgtccgg aaacatatat tgtgttgccg gcttttattc cttgagaata agggcctact
      481 gctgccggtg cattgttagt atgaataaca ctattcataa ttacctccta tttccaattt
      541 tttattccat attgttcaac atccaatgaa ttgttatcat accacaaaga ttccaggtca
      601 taaatttccc tttcatcctt gtgaaaaata tgaacaataa tgtcatcagt ttctaataaa
      661 atccatcttc cggtttcaaa accttcctta tagtaaaaat ctattccttc ctttgctcct
      721 ttttccaaca cttcttcagc aattgcaact gtctgtttct cgttgttccc gctgcaaatg
      781 atgaagtaat ccgtaatcga gctggtttta gacacattta aaacaacaaa gttataggct
      841 tttttattat cgcaagcttt taaaatagtt tcaatatttt tttgaacttc tatcatttac
      901 cctcctttaa ataattcagt gtatttactg attccatatc aagaaccaaa ttcctgtttt
      961 taacgtattc aattgtgttt tccaaagatt ttattattgc ttcatcaagt ctagtatatg
     1021 ctttatttct aatttcttca acaccttcat aattcctgtt tggctcaatt ttatctgcaa
     1081 tatatattat tttttccaaa agagacatat ttacttttcc cgttgtatga aacattattg
     1141 cattgagtat atcattgtct tttactccat actttacccc ggcaagctct gcactttttt
     1201 ttgcatgttt caaattgtcg ctgcatattc tttttccgca gtcatgcaat aatcctgcaa
     1261 ttattgcctt tgaaacatca caaccataaa ccgaagcaag tttttcagct tcttccattg
     1321 taccgatgct gtggcggtac agcttttcac ctaattcaat ttttaattct tttttcattt
     1381 cttcaatatg cattttactc tccgctgtct aaataatgtg atatccttca cttttcttgc
     1441 ccctttttat ataacccgtt cttaattata tattgctcca cagaatcagg taacagatat
     1501 tttatggaaa cacccatctt gatcctattt cttatatcgg aagaagatat ttgaagcatg
     1561 ggaacctgta aaatttctat atttccgcca aacattcttt ttatttcatt tatctttgtg
     1621 ttgacttcct caatactaat ccccggtctt gtagctgcaa taaacctgca aagctttaga
     1681 acttcttcag gctggtgcca gcttggaaga tttaaaacag catcagtacc ggttataaaa
     1741 taaaattctg tgtttttata cagcttgtgt aattccctta aagtactcaa ggtataactt
     1801 ttgctcgggt ttttaatttc aatatccgat acactgaatt tgtcattagt cagcactgcc
     1861 aaattaacca ttaacaatct gtgaaatgat tcagcattag gataatcttt atgcggcggg
     1921 tcacctgtcg gaataaaaat aatttcatct aatttatata tatttaatgc ttcatttgct
     1981 gcaactaagt gtcccatatg tataggatca aaagagccgc ccattattcc aacttttttt
     2041 ttcattgctc tccccttttt ctaataaaca taataacagc tgcaaataaa ataatactca
     2101 atgtaaatat atcttcatag ggtcttatgt tgttaaaaag caatttgctt attgaaaatc
     2161 ctaatattcc cgaaaaaata taaggaaaat ataattttat cttgctttgt ttaacatctt
     2221 ttattctttg aagcatactt tctcttccct gaaagtaacc attcataaaa ccgttgtaag
     2281 aaataaaaag tataaagact gacaggataa ttacaggtat aaacgccaaa taaaaactac
     2341 cgtctaaaca aagcataaca gacatggata tgaaaagcgg tgccaaaatt gcctgagtga
     2401 aagaatcccc caatcctgcc
//
LOCUS       DAOSOW010000297         2401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_44077_length_2401_cov_3.909633, whole genome shotgun sequence.
ACCESSION   DAOSOW010000297 DAOSOW010000000
VERSION     DAOSOW010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44077_length_2401_cov_3.909633"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="PLD67_07550"
     CDS             complement(<1..169)
                     /locus_tag="PLD67_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HOT22414.1"
                     /translation="MQGKVQKMDHHNPGIKCVVNTCYYYDEGDHCKAQKIEVQRRNAT
                     SSHETDCATFTK"
     gene            397..1470
                     /locus_tag="PLD67_07555"
     CDS             397..1470
                     /locus_tag="PLD67_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22415.1"
                     /translation="MKEKHIFAGNNTSEGFFSYFDYLLKPEEAEHIYILKGGPGVGKN
                     RFMKKFADEMAKKNYSIEYIHCSSDNASLDGILIPELKILIVDGTSPHTIDPVIPGAV
                     DEIINLGAFLNVQKLNKHKTAIIQTNKAKSNKYLSAYRYLKAAGIIYNEISSIYDLYV
                     DVEKFNEMCEKAITGLFAHSSDKKSDNVRKLFTESFTSNGYISYTEKFFEENEVWAVV
                     GNDTNYSSVFLDRILNEAIKKGYSVECYYRPLSPKKLQHIYLPEKKLILVTAENHMNE
                     KYKKVFDIHGIMDSESIKTRVSEIEKNLHLYDLLIRNALDKLSETKKLHDLLEVFYAD
                     SMDFERVNECFEKIVRGIGTQIL"
     gene            1575..>2401
                     /locus_tag="PLD67_07560"
     CDS             1575..>2401
                     /locus_tag="PLD67_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22416.1"
                     /translation="MALTSLHYLYMVMVVIILVIMLMKKEIVIPCIVGIFAMGFLYSG
                     SAVFAVQSIFNTIIYSGNEFWGIILIISLVVAMSKSLQAVGADALIMSPIKKIMVNQT
                     MAFWGIGVAMMIISWLVWPSPAVPLIGAVLLPAAVATGLPVIYAAVAMNLFGHGIALS
                     SDFFIQGAPTITAAGAGVDVPEIIGASIPIWLTMSAVTIATAFFLMKRDLKKSSQLTP
                     IANNNFKHEEITQSKTAKYVAVLTPIAFLIDIVAMVKFNLAGGDATALVGGTAIIIML
                     "
BASE COUNT          802 a          390 c          432 g          777 t
ORIGIN      
        1 ttttagtaaa tgttgcacaa tccgtttcat gtgatgaagt tgcattgcgc ctttgcactt
       61 caatcttctg tgccttgcag tggtcccctt catcatagta ataacatgta tttacaacac
      121 atttgatgcc gggattatga tgatccattt tttgtacttt tccttgcata atatgtctcc
      181 ttttttgtta ttgaaagtca aaactttcaa aattattatg ccctttaata ttttttttat
      241 aatatctatt tttttattac aaataaatac aaatctaaag aagaatttct ccttatgctt
      301 aatctttcag gatgtaatca ggaataataa aaaagaatta attttaacaa gaacagaaat
      361 cgttcatata atggaataac attgaggagg tagaaaatga aagaaaaaca tatttttgcc
      421 ggcaacaata catctgaagg atttttcagc tattttgatt atctattaaa gccggaagaa
      481 gctgaacaca tttatattct taaaggagga ccaggagtag gcaaaaatcg tttcatgaaa
      541 aaatttgcag atgaaatggc aaaaaagaac tattcaatag aatacataca ctgctcaagc
      601 gacaatgcaa gtcttgacgg aattttgatt cctgaactaa aaattcttat tgttgacggt
      661 acatcacctc ataccatcga ccctgtcatc ccgggagcag ttgatgaaat tattaatctc
      721 ggagcctttt taaatgttca aaaactaaat aaacataaga cagctataat ccaaaccaat
      781 aaagctaaat caaataaata tttaagtgct tacaggtatt taaaagctgc aggtattatc
      841 tataacgaaa tcagctcgat ttatgatttg tatgttgatg tcgaaaaatt taatgaaatg
      901 tgcgaaaaag caattaccgg actgtttgct cattcatctg ataaaaaaag cgacaatgtc
      961 agaaaattgt ttaccgaatc ttttacgtca aacggatata taagctatac cgaaaagttc
     1021 tttgaagaaa atgaagtgtg ggcggttgta ggtaatgata ctaactattc ttccgtattt
     1081 ctggacagaa ttttaaacga agcaattaaa aaaggatact cagtagaatg ctattacaga
     1141 cctttaagcc cgaaaaagct gcagcatata tatttgcctg aaaaaaaact tatacttgtt
     1201 actgcagaaa atcatatgaa tgaaaaatat aagaaggttt ttgatattca cggcataatg
     1261 gattctgaaa gtataaagac acgtgtttct gaaattgaaa aaaaccttca tttatatgat
     1321 ttattaataa gaaatgcctt agataagctt tctgaaacga aaaagctgca tgacctatta
     1381 gaagtattct atgctgattc catggatttt gaacgtgtta atgaatgctt tgaaaaaata
     1441 gtaagaggaa taggtacaca aattttatga cactaattta tatgtaaggg gacacaatta
     1501 cataaggcag ggggacacac ttataaaatt tccttgaagg aatgttcctc tgcttaattc
     1561 caaggaggtt ttatatggct ttaacttctc ttcactactt atatatggta atggtagtaa
     1621 taattcttgt tattatgctc atgaaaaaag aaattgtaat tccatgtatt gtcggtattt
     1681 ttgccatggg tttcctttac tcgggcagtg cggtatttgc tgttcagtct atatttaaca
     1741 caattattta ttcaggcaat gaattctggg gtattatact aataatttca ttggttgtcg
     1801 ccatgtctaa gtccctgcag gcggttggtg cagatgcgtt aattatgtcg ccaatcaaaa
     1861 aaataatggt taatcaaaca atggcatttt ggggaatagg tgtggcaatg atgattatat
     1921 cctggctcgt ttggcccagt cctgcagttc ccttgatagg agcagtcctg ctgcctgccg
     1981 cggttgccac aggcctgcct gttatttatg cggcagttgc tatgaattta ttcggtcatg
     2041 gcattgcact ttccagtgac ttttttatac aaggagctcc taccataacc gctgccgggg
     2101 cgggcgttga tgttcctgaa attattggag cttcaattcc catatggctt acaatgtctg
     2161 cagtgactat agcaactgca tttttcctga tgaaaagaga cttaaaaaag tcttcccaac
     2221 ttacacctat tgcaaataat aattttaaac atgaagaaat aactcaaagt aagacagcaa
     2281 aatatgttgc tgttttaact ccaatagcat ttttaattga tattgtggca atggtaaaat
     2341 tcaatttggc aggaggcgat gcaaccgcac ttgtaggagg aaccgcaata atcataatgc
     2401 t
//
LOCUS       DAOSOW010000298         2400 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_44097_length_2400_cov_4.194030, whole genome shotgun sequence.
ACCESSION   DAOSOW010000298 DAOSOW010000000
VERSION     DAOSOW010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44097_length_2400_cov_4.194030"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /gene="hutH"
                     /locus_tag="PLD67_07565"
     CDS             <1..1146
                     /gene="hutH"
                     /locus_tag="PLD67_07565"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOT22417.1"
                     /translation="EMLNRKVHPIIPEKGSLGASGDLAPLSHMVLPMIGLGQAEYEGE
                     IMAGEEAMEKAGIPVIELTAKEGLALINGTQVMTALAALTVYDSLNIIKTADIAAALS
                     FEAQNGIIDALDQRLHDIRPHKGQIDTARVLTELLTGSKMTTKQGEIRVQDAYSLRCV
                     PQVHGASKDAINYVKEKIEIEINSVTDNPIIFPETNEGISGGNFHGQPMALSLDFLGI
                     ALSELANISERRIERLVNPTLSGLPAFLVEHGGLNSGFMIVQYSAAALVSENKILAHP
                     ASVDSIPSSANQEDHVSMGTIAARKAKEIMENVRRVLAMEIMCACQGIDLRGNKGLGA
                     GTKPVYDEVRKVVPMLKEDRPIYDDINKCEELIKNGTITKACEGRIR"
     gene            1276..1857
                     /locus_tag="PLD67_07570"
     CDS             1276..1857
                     /locus_tag="PLD67_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOT22418.1"
                     /translation="MNNNERWLVEESRKGNVEAFEELIKDYKKTAYNIALRIMRNVED
                     AEDASQEALIKIFKNISSFNMESTFKVWMYRIIVNTCIDFKRRKNISTVSIDETMDLG
                     SGREVQREISDESYNPDALIERNYSTQLVNDAINALEDDFKTIIILRDIKGFTYDEIS
                     QILSCNLGTVKSRLSRARKRLRELLENEMKVQG"
     gene            1870..>2400
                     /locus_tag="PLD67_07575"
     CDS             1870..>2400
                     /locus_tag="PLD67_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOT22419.1"
                     /translation="MKCNEINDKLSLYIDNELSHEEMQQVEEHLHSCEKCQKILEEYK
                     NLISVLRNLPEEEPPVGYCKRLHEKLLESNPQSKTKKAASKIKSFNRNKWLKYGSLAA
                     ALVLVFVAANINNIGRMGMKAKNESYDMATTESPAEYYGAGSPAPEAPQAGPPVMTEE
                     AAEEGYDGDNKSARDDL"
BASE COUNT          888 a          389 c          537 g          586 t
ORIGIN      
        1 gaaatgctga acagaaaagt tcatccaatc atacctgaaa aaggctctct cggtgcaagc
       61 ggggatttag ctcctctgtc acacatggtg ctgcctatga tagggcttgg gcaggcggaa
      121 tacgaaggag aaataatggc aggcgaagaa gctatggaaa aagcgggaat acccgtaatt
      181 gagctgacag ccaaggaagg acttgctctt ataaacggga cacaggttat gactgccctg
      241 gcagcattga ctgtttacga ttcgttaaac atcataaaaa cagcggatat tgcagcagcg
      301 ttaagctttg aagctcaaaa cggaataatc gatgcattgg accaaaggct ccatgatatt
      361 cgtcctcaca agggtcaaat agatactgca agagtattga ctgagctgtt gacagggagc
      421 aaaatgacaa ccaagcaagg ggaaattcgt gtacaggatg cttattcctt gcgatgcgtt
      481 cctcaggtgc acggggcaag caaggatgca ataaattatg ttaaggagaa aatagaaata
      541 gaaataaatt cagttaccga taaccccata atattccctg aaacaaatga aggtatttcg
      601 ggaggaaatt tccacggaca gcctatggca ctctccttag actttttagg aattgcactt
      661 tctgagcttg caaatatttc cgaaagaaga attgaaaggc tggtaaatcc tacacttagc
      721 ggattacccg catttttggt agagcatggc ggattaaatt cggggtttat gattgttcaa
      781 tattctgcag ctgccttagt ttccgaaaat aaaatattgg cacatcctgc ttctgttgac
      841 agcattccat cctctgcaaa tcaagaagac catgtttcta tgggaacaat tgctgcaaga
      901 aaagcaaaag aaattatgga aaatgtgcga agagtcttgg ctatggaaat aatgtgcgct
      961 tgtcagggaa tagacctaag agggaacaaa ggattaggag cgggaacaaa accggtttac
     1021 gatgaggtaa gaaaagttgt tcccatgcta aaagaagaca gaccaattta tgacgatatt
     1081 aataaatgcg aagaattaat taaaaacggg acaattacaa aggcttgcga aggcagaata
     1141 agatagatgc taatcatcct gctgcctttt tattgattat aattaacttg aatgttataa
     1201 ttaaacaaaa tcaaaaattc gtggaacaaa ttaaaaaatg tttacgtcta atatataacc
     1261 gaacggggtg gaataatgaa taataatgaa aggtggcttg tagaggaaag tcggaaaggt
     1321 aatgttgaag cttttgaaga gcttatcaag gattataaga aaactgccta caacattgct
     1381 cttcgcataa tgagaaatgt tgaagacgca gaagatgctt ctcaggaagc actgataaaa
     1441 atatttaaaa atataagcag ctttaatatg gaatctacat ttaaagtatg gatgtataga
     1501 attatagtaa acacatgtat agatttcaaa cgcagaaaaa atataagcac agtatctata
     1561 gatgaaacaa tggatttagg ctccggaaga gaagttcaaa gagaaatttc agatgaatcc
     1621 tataacccgg atgctttaat cgaaagaaat tacagtaccc agcttgtaaa cgatgcaatc
     1681 aatgcgttgg aggatgattt taaaacaata ataatactga gggatataaa aggattcaca
     1741 tatgatgaaa tatcacaaat attatcctgt aatttaggca cggtaaagtc aagattgtca
     1801 agagccagaa aaagattaag agaactattg gaaaatgaaa tgaaagttca gggctaaggt
     1861 ggtgataaaa tgaaatgtaa cgaaataaat gacaaattgt cattatatat tgataatgaa
     1921 ttaagccatg aagaaatgca gcaagtggaa gagcatttgc attcatgcga aaaatgtcaa
     1981 aaaatcttgg aagagtataa aaatttaata tcagtattgc gaaatttgcc ggaagaagag
     2041 ccacctgtag gttactgcaa aagacttcat gaaaaattat tggagagcaa ccctcagtca
     2101 aaaacaaaaa aggcagcttc aaagataaaa agtttcaatc gcaataaatg gctgaaatac
     2161 ggcagtcttg ccgctgcact ggtattggtg tttgtagcgg ccaatataaa caatataggc
     2221 agaatgggaa tgaaagctaa aaatgaatcc tatgatatgg caacaactga atcaccagcc
     2281 gaatactacg gtgccggctc gcctgcccca gaagcacccc aagcaggacc gccggtaatg
     2341 actgaagaag ctgcggaaga ggggtatgac ggtgataata agagcgcaag agacgacctt
//
LOCUS       DAOSOW010000299         2388 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_44404_length_2388_cov_3.820832, whole genome shotgun sequence.
ACCESSION   DAOSOW010000299 DAOSOW010000000
VERSION     DAOSOW010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44404_length_2388_cov_3.820832"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..1180)
                     /gene="ftsW"
                     /locus_tag="PLD67_07580"
     CDS             complement(59..1180)
                     /gene="ftsW"
                     /locus_tag="PLD67_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOT22420.1"
                     /translation="MEETVRKAKARSIDWVLVFIIVLLVLFGFLMVYSSSYPDGYYKY
                     GGDPFYFLRKQVIAAGAGLAGMIFFANLPYIIYKKFSKLILFTCIGLAMLTIIIGIAS
                     NGAQRWIEIGGISLQPSEIIKIGAVLYMADYFNRKRSELPSFTKGFLPSLFYIVLFCG
                     LIAIQDDLSTAVILAGTLMVMFFCAGARISHLILLFLLALAGIIALIAMQPYRISRII
                     VFFDPFKYPLDEGYQIINSLYALGTGGLFGMGVGKSRQKFFHLPEAYNDFIFSIIGEE
                     LGFVGAVFVMLGFVVFAWRGLRISMNTEDFFGSQTALGLTVIVLVQFLIHVAVATSSM
                     PPTGRALPFISAGGSSIMFLLCSMGILLNISKYSNTYRS"
     gene            complement(1155..>2388)
                     /gene="murD"
                     /locus_tag="PLD67_07585"
     CDS             complement(1155..>2388)
                     /gene="murD"
                     /locus_tag="PLD67_07585"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOT22421.1"
                     /translation="EDIEVEYFFGNEEINKIKFGNLDYAIKSPGIKYETPIIEKLVKN
                     NIEVISDIEAAYRVTNSPIVSITGTNGKTTTTTLIGEMVRESKKTYKVTGNIGFGIYY
                     DALTAKEEEILVAEVSSFQLAGTSKYKPHISVITNITPDHLDYHHTVENYIEAKFKNV
                     INQDENDYAILNYEDETIRNYSDKIKAKKIFFSSERILEDGIFSEKGQMFYRTGNNKL
                     SIINTKDIFIPGKHNLENAMAAGGAAIALGIDIEVISRVLREFKGVEHRLEFTGEYKK
                     VKFYNDSKGTNPDASIKAIQGIEKPIILIAGGYDKKSSYDDFIKAFDNKVKALILLGQ
                     TAEDIQKCALKYGFENIYRVETMDEAVKKSFELAEEGDNVVLSPACASWGMYPNYEVR
                     GRDFKERVEYYGRNRKKS"
BASE COUNT          709 a          502 c          365 g          812 t
ORIGIN      
        1 tgagagcttt agctcgaaga atctcatgag atccttcgct tcgctcagga tgacataact
       61 atgacctgta agtattggaa tatttcgata tattaagcaa aatccccatg gagcagagca
      121 aaaacattat cgatgagcca cctgcgctta taaagggaag ggctcttccc gttggtggca
      181 tcgaagatgt tgcaaccgct acatgaatta aaaactgaac cagtactatt acagtcaagc
      241 ccaaagctgt ctggcttccg aaaaaatctt ctgtgttcat tgaaatccta agtcctctcc
      301 atgcaaatac cacaaatccc agcattacaa agaccgctcc aacaaagccc agctcttctc
      361 ctattataga gaaaataaag tcattgtatg cttccggcaa atgaaaaaac ttttgtctgc
      421 tttttcctac tcccatacca aataatcccc cggtacccaa ggcatacagt gaatttatta
      481 tttgataacc ctcatccaag ggatatttaa aagggtcaaa aaagactatt atacgtgata
      541 ttcgatatgg ctgcattgca atcagtgcga ttatgcccgc gagtgcaaga agaaacaaca
      601 gtattaaatg gctgattctt gctcctgcac aaaaaaacat aaccatcaga gttccggcta
      661 atattacagc tgtgcttaaa tcatcctgta tagcaattaa cccgcaaaat agcactatat
      721 aaaacaacga tggcaaaaaa cctttggtaa aggatggcaa ttcacttctt tttctgttga
      781 aataatcagc catataaagt acagctccga ttttaattat ttccgagggc tgcaatgata
      841 ttccgccaat ctctatccac ctctgggcgc catttgatgc aattcctatt ataatggtaa
      901 gcattgctaa tcctatacat gtgaataata tcagcttact gaattttttg tagattatgt
      961 atggaagatt tgcaaaaaat atcattccgg caagtcctgc tcctgctgca ataacttgtt
     1021 ttcgtaaaaa ataaaaagga tctcccccat atttataata tccgtcggga taagatgagc
     1081 tgtaaaccat taaaaatcca aacaagacca ataatactat tataaaaacc aatacccaat
     1141 caattgacct ggctttagct tttcttacgg tttcttccat aatattctac cctttcttta
     1201 aaatctctgc ctctgacttc ataattaggg tacatccccc agctggcgca tgccggggat
     1261 aaaacaacat tgtctccttc ttcggccaat tcaaagcttt tcttcacggc ttcatccatt
     1321 gtttcaactc tgtaaatatt ttcaaagcca tattttaaag cacacttttg aatatcttcg
     1381 gcagtctgcc caagaagtat caatgctttt accttattgt caaatgcctt aataaaatca
     1441 tcgtaggatg acttcttatc atatccgcct gctatcaaga taatcggttt ttcgattcct
     1501 tgaattgcct taattgatgc atcgggattt gttccttttg aatcattata gaatttaacc
     1561 ttcttgtatt cgccggtaaa ttcaagacga tgctcaacac ctttaaattc tcttaaaact
     1621 cttgatataa cttcaatatc aattccaaga gctattgcag caccgccggc agccattgca
     1681 ttttctaaat tgtgtttccc cggaataaat atatcctttg tattgataat ggatagttta
     1741 ttgttaccgg ttctgtaaaa catttgaccc ttttctgaaa atattccatc ctccaaaatt
     1801 ctttcggagc tgaagaatat tttttttgcc tttattttat ctgaataatt cctgattgtt
     1861 tcgtcttcat aatttaatat tgcataatca ttttcatctt gattaattac atttttaaat
     1921 ttcgcttcta tataattttc taccgtgtga tgatagtcta aatggtcagg agtaatattg
     1981 gttattacgc ttatatgagg tttgtacttt gaagtacctg ctaactgaaa agaacttacc
     2041 tcagccacaa gaatttcttc ttcctttgcc gttaatgcat cgtaatatat tccaaaacct
     2101 atatttcccg ttaccttata ggtttttttg ctttctctaa ccatttcgcc aattagagtt
     2161 gtggttgttg ttttgccgtt agttcccgtt atggaaacga tgggtgaatt tgtcaccctg
     2221 taagccgctt ctatgtcact tattacttca atattgtttt taacaagttt ttcgataatc
     2281 ggtgtttcat atttgatacc ggggcttttt atggcataat ccaaattccc gaattttatt
     2341 ttatttattt cttcattgcc gaagaagtat tcaacttcta tatcttct
//
LOCUS       DAOSOW010000300         2380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_44648_length_2380_cov_3.192688, whole genome shotgun sequence.
ACCESSION   DAOSOW010000300 DAOSOW010000000
VERSION     DAOSOW010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44648_length_2380_cov_3.192688"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..>2380)
                     /locus_tag="PLD67_07590"
     CDS             complement(74..>2380)
                     /locus_tag="PLD67_07590"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOT22422.1"
                     /translation="SMEASGYNIFVLGPQGTGKTTYSQTVVSKAASKRKVPDDWCYIN
                     NFSEWDKPLAISLPAGQGKVFQKDMEKLITNLVVYIPKAFESSNFQQQKDDIIQKTNK
                     QMTEILRVIDKIARNAGFGIQQAANRILLIPLVENRQITQEEFSRLTEEEREKIDEKR
                     NQTAKEIDDRIRDGQMVQRLAEEKAIEIQKQTAKEAAEPFIEQLKEKYKNFPEIADYL
                     DKVLKDAAENHVIFSSVRHSLNENIITKLQDDSDSLDGEEKNIFEKAASARDSKDPFT
                     RYKVNLFINNENTKGAPVITESSPYYYNLFGKIEYKSHMMTTMTDFTMIKPGAIHKAN
                     GGYLILQAKDLLMDPFAWGALKKALKYKEAVVENIGEQSRYVPTVTLKPQPIPLNVKV
                     ILIGSYYYYQFLAADEDFRKLFKVNVDFDVEMDRTRENIQNYVSFIASMCENENLKHF
                     NCEGLAKVVEYGSRLADNQNKLSTRFNIVSEIVYEASALAEADNSEYVEGKHVNQAIK
                     NKKYRSNRIEEKMQEQIVNKKVLIDTEGAVVGQVNGLSVMGVSGYAFGLPSRITARTY
                     AGREGIINIERETDMSGNIHSKGVLTLNGYLGGKFAQEKPLGLTAQVTFEQLYGGVEG
                     DSASSAELYAILSSLSDVPIKQNLAVTGSVNQMGEIQPIGGVNEKIEGFFDICSLNGL
                     TGDQGVIIPVSNIDNLMLKDEVIEAVKNNLFHIYAVTKIEEGIELLTGVKAGEKDIYG
                     DYEKDSIFYLANNKINEFNKVLKTESEA"
BASE COUNT          575 a          514 c          394 g          897 t
ORIGIN      
        1 gagatccttc gctccgctca ggatgacaaa aaggtttcgc cttaattaca acacgggctt
       61 gcgcccgtgt tatttatgct tctgattctg ttttcaatac tttattgaat tcatttattt
      121 tattgtttgc aaggtagaat atgctgtctt tttcatagtc gccgtaaata tctttttctc
      181 ctgccttgac tcctgttaaa agttcaattc cctcctcgat ttttgtaacg gcataaatgt
      241 ggaagaggtt gtttttaaca gcttctatca cttcatcctt tagcatcagg ttgtctatat
      301 tgcttacggg tattatgact ccctggtctc cggtaagtcc attcaatgag cagatgtcaa
      361 agaaaccttc aattttttca ttaaccccgc ctatgggctg aatttctccc atttgattta
      421 cagagcccgt aacagctaaa ttttgtttga tgggtacatc agacaagctt gatagtattg
      481 catacagctc tgcgcttgaa gcactgtctc cttcaactcc tccgtaaagt tgttcaaagg
      541 ttacctgagc agttaatccc aatggttttt cttgagcaaa ctttcctccc aagtaaccgt
      601 ttaaggttag aactcccttg gaatggatat taccgctcat atccgtttct ctttctatat
      661 ttataattcc ttccctgccc gcataagttc ttgcagtgat tctggacgga agtccaaagg
      721 cataaccgga cactcccatt acggaaaggc cgttcacctg ccctacaact gctccttcag
      781 tatcaatcag aacttttttg ttgactatct gctcctgcat tttttcttca attctgttgg
      841 agcggtattt tttatttttt attgcctgat tgacgtgctt gccttcaacg tattctgaat
      901 tgtcggcttc tgccaaggct gaagcttcat atactatttc acttacaata ttgaaacgtg
      961 ttgacagttt gttttgatta tctgcaagtc ttgagccgta ttccaccacc ttagccagtc
     1021 cttcgcagtt aaaatgcttt aaattctcat tttcacacat tgaagcaata aatgacacgt
     1081 aattttggat attttcccgt gtcctgtcca tttctacatc aaagtctaca tttactttaa
     1141 agagctttct gaaatcttca tctgcagcta aaaactgata gtaataatag ctgcctatta
     1201 atatgacttt tacatttaaa ggtataggtt gaggtttcaa tgttaccgta ggaacatacc
     1261 ggctttgctc ccctatgttt tccacaacag cctctttata cttcaatgcc tttttaagtg
     1321 cgccccacgc aaaggggtcc attaacaaat ccttagcctg tagaattaag tatcctccgt
     1381 ttgctttatg aattgctccc ggttttatca tcgtaaaatc cgtcatagtg gtcatcatat
     1441 gggatttgta ttcaatttta ccgaataaat tgtaataata aggactgctt tccgttataa
     1501 cgggtgctcc ctttgtattt tcattgttta taaacaaatt taccttgtat cgtgtaaaag
     1561 ggtccttgct gtcccgtgct gacgctgcct tttcaaatat atttttttct tctccatcta
     1621 aggaatccga atcatcttga agtttagtta ttatattttc attgagacta tgccttacac
     1681 tgctgaaaat tacatgattt tccgcagcat ccttcagtac cttgtctaaa tagtctgcaa
     1741 tttcgggaaa atttttatat ttttctttta gctgttcaat aaaaggctct gcagcttctt
     1801 ttgcagtttg tttttgaatt tcaattgctt tctcttctgc aagcctttgc accatttgac
     1861 cgtctcttat tctatcgtct atttcctttg ctgtttgatt tcttttttcg tctattttct
     1921 cacgctcttc ttcggtgagt ctactgaatt cctcttgtgt aatctgcctg ttttcaacca
     1981 atggtataag tagaattcta ttggctgcct gctgtatgcc aaatcctgca tttcgtgcaa
     2041 ttttatctat aactcttaaa atttctgtca tttgtttatt tgtcttttgt attatatcgt
     2101 ctttttgctg ctggaagttg ctgctttcaa acgctttcgg aatatataca accagatttg
     2161 ttataagctt ttccatatct ttttgaaaaa ctttaccctg tcctgcagga agggaaattg
     2221 caagaggttt atcccattca ctgaagttat tgatataaca ccagtcatca ggtactttac
     2281 gcttggatgc agcttttgat accactgtct gcgaataagt agtcttgcct gttccctgtg
     2341 gtcctaatac aaaaatatta tagccggaag cttccatgga
//
LOCUS       DAOSOW010000301         2380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_44651_length_2380_cov_3.060215, whole genome shotgun sequence.
ACCESSION   DAOSOW010000301 DAOSOW010000000
VERSION     DAOSOW010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44651_length_2380_cov_3.060215"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="guaA"
                     /locus_tag="PLD67_07595"
     CDS             complement(<1..898)
                     /gene="guaA"
                     /locus_tag="PLD67_07595"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOT22423.1"
                     /translation="MAELILVLDFGGQYKELIARVTRGMNVYSEIKPGTLSAIEIKKL
                     APKGIILTGGPNSVYLDNAPKCDPDIFHLGIPVLGICYGMQTMCHSLGGKVEPCAVNE
                     YGRIKADFDMNSKLFAEVKQNSYVLMSHSDCVTELPKGFVDIAKTYDCKYAAVENQNK
                     KLYGVQFHPEVQHTDSGKQIIRNFLFNICGVSGDYHLDDYLDRQIELIRNKVGKEKVL
                     LGLSGGVDSSVCAALLQKAIPGQLTCIFIDHGFMRLNEGDEIEKAFAGKKLNFIRVNA
                     QERFLNKIKGITDPEQKRKLIGE"
     gene            complement(1022..2293)
                     /locus_tag="PLD67_07600"
     CDS             complement(1022..2293)
                     /locus_tag="PLD67_07600"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOT22424.1"
                     /translation="MLTAIVGINWGDEGKGRMVDLLSSEYDIICRYQGGNNAGHTVVN
                     EKGKFILNLLPSGILRDTTVNVMGNGMVIDIEHLCNEIKALQNKGIKVTPKNLKISDK
                     ATICMPYHKLQDNLEEDRLGDKKFGSTRRGIAPVYADKYMKKSIRTGDLLSPGTLEDK
                     VSNIIEWKNITLDGYKKPKISKESMMQWLSEYSTPLLPFITDTTEYLLAAVKENEDIM
                     FEAQLGALRDIDFGIYPYTSSSSTLASYAPIGSGIPGVKIDKTIGIMKAYSSCVGEGP
                     FATELFGDEGSKLREAGQEYGAATGRPRRVGAFDIVASNYGVKMQGADELALTKLDVL
                     SYLDKIPVCVAYEIDGEKTTVFPTGDKLLRAKPVYEYLEGFKTDISSCRKLEDLPKAA
                     IDYIRFIENAVNCPIKYVSVGARREDYLTLG"
BASE COUNT          668 a          521 c          378 g          813 t
ORIGIN      
        1 cttctcctat cagttttcgt ttttgctccg ggtctgttat accttttatt ttatttaaaa
       61 atctttcttg tgcgtttacc cttatgaagt ttaacttttt accggcaaat gctttttcta
      121 tttcgtcacc ttcattcagt ctcataaaac cgtggtcaat aaaaatacaa gtaagctgcc
      181 cgggtattgc tttttgtaac aatgcagcac aaaccgaact gtcgacaccg cctgacagac
      241 ctagtaaaac tttttctttt ccgactttat ttctgataag ttctatttgt ctatccaaat
      301 aatcatcaag gtgataatcg ccggagacac cgcaaatatt gaaaaggaaa ttacggataa
      361 tctgcttacc gctgtctgta tgttgcactt caggatgaaa ctgtacgcca tacagttttt
      421 tattttgatt ttctactgct gcatatttac aatcatatgt ttttgctatg tcgacaaacc
      481 ccttgggcaa ttctgttaca cagtcggaat ggctcataag tacatatgaa ttttgtttta
      541 cttcagcaaa taattttgag ttcatatcaa aatcagcttt tattcttccg tattcgttga
      601 ctgcacaagg ttcaacctta ccgcccaagc tatgacacat agtctgcata ccgtaacaaa
      661 tccccaaaac aggaatgccg agatgaaata tatccgggtc gcatttcggt gcattatcca
      721 aataaacgct gttaggtcct cctgttaaaa ttattccttt aggagctaat ttttttattt
      781 caatagcaga aagtgttcct ggtttgattt cggaatacac attcattcct cttgttactc
      841 ttgctataag ttccttatac tgaccgccaa agtcaagcac taaaataagc tctgccatat
      901 ttccaccact ctttcaaaca acctgtgttc ctgtataaaa ttttttatat cagacataat
      961 tttctcctgt aataatttaa tgttgttcat atactttaat tttgataaaa ttaaagttgg
     1021 tctaacctaa tgtcaaataa tcttccctgc gtgcacctac agatacatac ttaatcggac
     1081 aatttactgc attttcaata aaacggatat aatcaattgc tgctttcggc aaatcttcca
     1141 attttctgca acttgatata tcggtcttaa aaccttcaag atattcataa acgggctttg
     1201 ctcgaagcag cttatctcct gtggggaaaa ctgtagtctt ttcgccatct atttcgtatg
     1261 caacacaaac aggaatttta tccagataag acagaacatc gagtttagtc aaagccagtt
     1321 catctgcacc ttgcatttta acaccgtaat tggaagctac aatatcaaaa gcgcctaccc
     1381 tgcgcggtct gcccgttgca gcaccgtatt cctgtccggc ttctcttagt ttcgaaccct
     1441 catcaccaaa aagttctgtt gcaaacggtc cttctccgac acagcttgaa tatgctttca
     1501 taattccaat agttttatca attttaactc cgggtattcc tgaacctata ggagcataag
     1561 atgctaatgt ggaggaagat gaagtataag gataaatccc gaaatctata tctcgtaaag
     1621 caccaagctg ggcttcaaac attatatcct cattttcttt tacagcagcc agcagatatt
     1681 cggttgtgtc ggttataaag ggtaatagcg gtgtgctgta ttccgagagc cactgcatca
     1741 tagactcttt tgaaattttt ggttttttgt agccgtcgag agtaatgttt ttccattcga
     1801 taatgttcga tactttatct tctagtgttc ccggcgataa taaatcacca gtacgaatac
     1861 tttttttcat atacttatcc gcataaacgg gagcaattcc tcgtctggtt gaaccgaatt
     1921 ttttatcgcc gagcctatct tcttcaagat tatcctgcag cttatgataa ggcatgcata
     1981 ttgttgcttt atcgcttatt ttaagattct taggcgtaac ttttatccct ttgttctgaa
     2041 gcgcttttat ttcattgcat aaatgctcaa tatcaatgac catgccgtta cccataacat
     2101 tgacggttgt atctcgcagt atgcccgatg gcaaaagatt taatatgaat tttccttttt
     2161 cgttaacaac agtatgaccg gcattattac cgccttggta acggcaaatt atgtcatact
     2221 cggaggacaa taaatctacc atacggcctt ttccctcatc gccccagtta ataccaacta
     2281 ttgcagtaag catgatacta cctccttaaa ctttaatatt gactttaact cccaacaaat
     2341 ctctgttaga gtcaagcacc ggttttactt cgctcaaaaa
//
LOCUS       DAOSOW010000302         2375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_44780_length_2375_cov_3.000000, whole genome shotgun sequence.
ACCESSION   DAOSOW010000302 DAOSOW010000000
VERSION     DAOSOW010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44780_length_2375_cov_3.000000"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PLD67_07605"
     CDS             <1..863
                     /locus_tag="PLD67_07605"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HOT22425.1"
                     /translation="QLLWQNISVKFLEVISCNKSSILVSMTVKTNKAPEEAESTITLI
                     VRNISGAKLRCVEKIVISPNYYVLKFKNIKKIKIRTYSAAAVKDGSTVSGDNFAYAGR
                     ADKYYTVLCDGIGSGEEAYCESNGAVDLMSRFLYSDFTEEQILRTLNSVLMIKLGDER
                     YVTFDFTVIDYGTKEIRLYKAGAAPSYIISGKNVDKISGKSLPLGILDNFEYCSFKKE
                     INIGDMVVMVTDGIIDSISLDPKKSLDKYLELLAGKDPQTVANSILSYALRGQDKIID
                     DMTVLVTKIG"
     gene            1114..>2375
                     /gene="tilS"
                     /locus_tag="PLD67_07610"
     CDS             1114..>2375
                     /gene="tilS"
                     /locus_tag="PLD67_07610"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOT22426.1"
                     /translation="MHSLIKNNILEKNLISPGENILIALSGGPDSVFLFHNLRRLRET
                     LSFNLYASHINHMYRGKDAMHDEEFVRNLCDKYGIKLFVKRKNATEYAKELKVTEEEA
                     GRVLRYNFFKENLREIGEGKVALAHNLNDQAETVLQRIIRGTGIDGLSAMSFQKDNLI
                     RPMLNISRYEIMNYLHENNYEYCIDITNTQDIYGRNKIRLNLIPYLEQNFNPNIQSSL
                     YRMSEAMERDKKIIEKYIESKFSEALKEKSDMRIVLDLNLLKALDIGEIGRIIRRGIE
                     ELKGNTVNIEMKHIDSAINLISSGKTGKRINLTDGFTVEISYNNFIVNKTVEKVPDFE
                     YNIVLNRLNYIEEINKTLFATVSDIKDMSEVNIKDKDIVSIDYNLIKGSLVARNRRPG
                     DSMIPCGMMGSKKIKDIFIDLKIPKEER"
BASE COUNT          908 a          314 c          479 g          674 t
ORIGIN      
        1 aacagctttt atggcagaat attagtgtca agtttcttga agtaatatct tgcaataaat
       61 cttccatatt ggtaagtatg acggttaaaa caaataaagc acctgaagaa gctgaaagta
      121 caattacttt aattgttaga aatatttcgg gtgcaaagct aagatgtgtc gaaaagattg
      181 ttatatcacc aaattactat gtattaaaat ttaaaaatat taaaaaaata aaaataagaa
      241 catacagtgc agcagctgtt aaggacggct caacggtgtc gggagataat tttgcatatg
      301 cgggaagagc agacaagtat tataccgttc tttgcgacgg catcggctcc ggtgaagaag
      361 catactgcga aagtaacggc gcggttgatt tgatgtcaag gttcttatac tctgatttca
      421 cggaagaaca aatattaaga acattgaatt cggtactgat gattaaactc ggtgacgaaa
      481 gatacgttac atttgatttc acggtaatag attacggaac aaaggaaata aggctgtaca
      541 aggcgggagc tgcgccttca tatataataa gcggcaaaaa tgtggacaaa ataagcggca
      601 agagtctgcc cctgggaatt cttgataatt ttgaatactg ctcctttaaa aaagaaatca
      661 atattggaga tatggtggta atggtcaccg acggaattat tgattcgata agcctggacc
      721 ctaaaaagtc attagacaaa tatttggaat tactggcggg aaaagacccc caaactgtcg
      781 caaattccat attaagctat gcacttagag gccaagataa aataatcgac gacatgacag
      841 tcctggttac aaaaatcgga tagatagact atttcattta tgacattgtt atgaataaaa
      901 tacgtgtcat tctgagagct ttagctcgaa gaatctcatt ttataagacg tgagatcatt
      961 cgcttacgct caggatgaca aaaacgagga cgcaaattct aagcgatagc gaaggaaacg
     1021 ggaatttccc gtttttttta agttttttga aaaaatattt ttaaaatcat atatttgtgg
     1081 tataatttat gctatatagc attgaggaaa aatatgcata gtttgattaa aaataatata
     1141 ctagagaaaa atttaatatc tcccggagaa aatattttga ttgctttgtc cggaggaccc
     1201 gattcggtgt ttttgtttca caatttaaga aggcttaggg aaactttaag ctttaatttg
     1261 tatgcttctc atatcaacca tatgtacaga ggcaaagatg caatgcatga tgaagaattc
     1321 gtcaggaatc tttgtgacaa atatggtatc aaactttttg taaaaagaaa aaatgcaaca
     1381 gaatatgcta aggagctaaa agtaaccgaa gaagaagcag ggagagtttt aagatataat
     1441 tttttcaagg aaaatttaag agagattggg gaaggcaagg tagcacttgc tcataatttg
     1501 aacgaccagg ctgaaacagt actgcagaga atcataagag gaaccggaat tgacgggtta
     1561 agcgcaatgt catttcaaaa ggataattta ataagaccaa tgcttaatat ttcaagatat
     1621 gaaataatga attatctgca tgaaaataat tatgaatatt gtattgatat tacaaatact
     1681 caggatatat acggaagaaa taaaataaga cttaatttaa ttccatacct agagcaaaac
     1741 tttaatccaa acattcaatc ttccctttac agaatgtctg aagcaatgga aagagacaaa
     1801 aaaattatcg aaaaatatat tgaatcaaaa ttctcagagg cgcttaaaga aaaaagtgac
     1861 atgaggattg tattggattt aaatttgcta aaagctctgg atattggaga gattggaaga
     1921 attataaggc gcggaattga agaattgaaa ggcaataccg taaatattga gatgaaacat
     1981 attgacagtg ccatcaattt aataagctcg ggaaaaaccg gcaaaagaat taatttaacc
     2041 gatggtttta cggttgaaat aagctataat aattttattg tcaataaaac agttgaaaaa
     2101 gtaccggatt ttgaatataa tattgttttg aacaggttaa attatattga ggaaataaat
     2161 aaaactttat ttgcaacggt ttcagacata aaagatatgt ctgaggtaaa tattaaggac
     2221 aaggatatag taagtattga ttataattta ataaaaggaa gcttggttgc aagaaacaga
     2281 aggccggggg attcgatgat accttgcggc atgatgggaa gcaaaaaaat aaaagatatt
     2341 tttattgact tgaaaattcc gaaagaagaa agaga
//
LOCUS       DAOSOW010000303         2369 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_44926_length_2369_cov_3.710026, whole genome shotgun sequence.
ACCESSION   DAOSOW010000303 DAOSOW010000000
VERSION     DAOSOW010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44926_length_2369_cov_3.710026"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="PLD67_07615"
     CDS             complement(<1..424)
                     /locus_tag="PLD67_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22427.1"
                     /translation="MNNYRNNMPHYDSLPHNYNPMGINEHKNSNRMHLANTMPRMPGM
                     PDDMLQRPFIPETEMPLEIDGDMPALPRFPQQGIPSLIPPWLTQPEIPMMPGMQPTPG
                     MPGMPQMPGMQPTPGMPGMPQMPGMQPTPGMPGMPQMPG"
     gene            664..1062
                     /locus_tag="PLD67_07620"
     CDS             664..1062
                     /locus_tag="PLD67_07620"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HOT22428.1"
                     /translation="MINILLVTHGEFGKELLKSSEMIIGEVENAKSISFNHGDSFETL
                     LKKVEENIKNISDDELIVFTDMYGGSPYNAVNRAMKNRNFYHITGINFPLFIDIAVAR
                     DTYSLEEIAEKIIKNGKKSIVFVNEKFLSD"
     gene            1071..1532
                     /locus_tag="PLD67_07625"
     CDS             1071..1532
                     /locus_tag="PLD67_07625"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HOT22429.1"
                     /translation="MKNVFVRIDDRLLHGQVVVSWIPYLRAKEVVIADDEYANDEFIS
                     ELIKSSGPEGVIVHVKTIDETAAYLSDDNQNKILVLLRSIEGIKKLIKKTKISSINIG
                     GVGAAQGRKRYYNSIHLSDDELNILNDIANDNIYVEIRILPKDKAIIIKGN"
     gene            1534..>2369
                     /locus_tag="PLD67_07630"
     CDS             1534..>2369
                     /locus_tag="PLD67_07630"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOT22430.1"
                     /translation="MTFSDYCLLSIFYFWASSTAFSVGIGYYTIYRPIMAGMAAGLIL
                     ADIKTGMLAGAVVNIIFIDFISTGGSLKGDQCLTAILAAAASIIFKLNPLEAAAAAFP
                     FGFLGILIWKYRLKINNIFVHIYEKKYKENKAPDISLYNGLFPQLLLFMMSLAVIITA
                     FYLMFLFNKLIHFNSISVLLYYIGIILVIFSVSNVLYKIKIKYNYAIFISVFLAALVL
                     DMRAYILFILIVLSLLYLSEKKVSIVRNSFNGKLKRGDLVYSWFIWMNYSHSCYSYER
                     LMG"
BASE COUNT          788 a          322 c          489 g          770 t
ORIGIN      
        1 taccgggcat ttgcggcatt ccgggcattc cgggagtcgg ctgcataccg ggcatttgcg
       61 gcattccggg cattccggga gtcggctgca taccgggcat ttgcggcatt ccgggcattc
      121 cgggagtcgg ctgcataccg ggcatcatgg gtatttcggg ttgtgtcaac catggcggta
      181 ttaaactagg aatcccttgt tgcggaaacc tcggcagagc aggcatatct ccgtcaattt
      241 ctaaaggcat ttccgtttcc ggaatgaatg gcctttggag catatcgtca ggcatccccg
      301 gcattctggg cattgtgttt gcaagatgca ttctattaga atttttgtgc tcatttatgc
      361 ccattggatt atagttatgg ggcagtgagt cgtagtgggg catattattt ctataattat
      421 tcatactatc ctccattaaa taaatttctc agtattaata tacgtttgaa aaaaatatta
      481 gtgaattaaa aataaagaat tctttactat tttaataaat tcatataaac tattagtcaa
      541 aggaaacagg gttgaggaga atatctttac tatgttacaa taaaagacgg gcggaaaaag
      601 caatctaaaa tattgatgaa aattatgtat aattatggta ttatataaca aggagatgaa
      661 agtatgataa atatattact tgtgacacat ggagagtttg gtaaagaatt attgaaaagc
      721 tctgaaatga ttataggaga agtagaaaat gccaaaagta tatcatttaa ccatggtgac
      781 agttttgaaa cattgttaaa aaaggttgaa gaaaatatta aaaatatatc tgatgatgaa
      841 cttatcgtat ttacggatat gtacggcgga agtccttaca atgcagtaaa tcgtgcaatg
      901 aaaaacagaa atttttatca tataacaggt ataaacttcc ccttgtttat cgatattgct
      961 gttgctcgcg atacatacag cttagaggaa attgcagaaa aaataattaa aaacgggaag
     1021 aagagcatag tgtttgtcaa tgaaaagttc ttatcagact gaggtagaaa atgaaaaatg
     1081 tattcgttag aattgatgat agattgttgc acggacaggt tgttgtatct tggatacctt
     1141 accttagagc aaaagaggtt gtaattgcgg atgatgaata tgcaaatgat gaatttatta
     1201 gtgaactcat aaagagttca gggcctgaag gtgttatagt tcatgtgaag acaatcgatg
     1261 aaacggctgc ttatttaagc gatgacaatc aaaacaagat actggtactt ctcaggagta
     1321 ttgaaggtat aaaaaaatta ataaaaaaaa caaaaataag cagtataaac ataggtggag
     1381 tgggagcagc ccagggaaga aaaagatatt ataattccat tcatttaagt gacgatgaat
     1441 taaatatatt aaatgacata gcaaatgaca atatatatgt tgaaataaga atccttccaa
     1501 aggataaggc tatcataata aaaggtaatt gacatgactt tttctgatta ttgtttactt
     1561 agcatttttt atttttgggc aagcagtact gcattttccg taggtatcgg ttattataca
     1621 atatacaggc ctataatggc aggaatggct gcaggtttaa ttcttgctga tataaaaaca
     1681 ggcatgttgg caggcgcagt agttaatatt atttttattg actttatttc taccgggggt
     1741 tcattgaaag gagatcaatg tctaactgct atattggcgg cagcagcttc aataatattt
     1801 aagcttaatc ctcttgaagc agcagcagca gcttttccct tcgggttttt agggatattg
     1861 atatggaaat acaggttgaa aataaataat atttttgtac atatttacga gaagaaatat
     1921 aaagagaaca aagctcccga cataagtttg tacaacggac tttttccgca gctgctgtta
     1981 tttatgatga gtttggcggt tattataacg gcattctatt taatgttttt attcaataaa
     2041 ttaatacatt ttaacagtat tagcgtattg ctttattata tagggataat actggttatt
     2101 ttttccgttt caaatgtttt atacaaaatt aagattaaat ataattatgc aatttttatt
     2161 tctgtttttt tggcagcctt agtattagat atgagagcat atatactgtt tattcttata
     2221 gtattgagcc ttttatattt gtcagagaag aaagtgagca ttgtcaggaa tagttttaac
     2281 ggtaaattaa aaagaggaga cttggtttat tcatggttta tatggatgaa ttattctcat
     2341 tcatgctaca gctatgaaag acttatggg
//
LOCUS       DAOSOW010000304         2359 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_45169_length_2359_cov_4.180990, whole genome shotgun sequence.
ACCESSION   DAOSOW010000304 DAOSOW010000000
VERSION     DAOSOW010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45169_length_2359_cov_4.180990"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /gene="nhaC"
                     /locus_tag="PLD67_07635"
     CDS             complement(<1..1254)
                     /gene="nhaC"
                     /locus_tag="PLD67_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HOT22431.1"
                     /translation="MSKVPREKKEATLGISLIPVLVIVAVLAYSIIVLGADPHIPILV
                     GAAVGSLIAVFGLGYSWEEVEKGIIDSIGSVMQAIIILAIIGMLIGTWIGGGVVPTLI
                     YYGLQILSPTFFLITALLLCSVVSLATGSSWTTAGTVGVALIGIAQGLGVSPALAAGC
                     IVSGAYFGDKMSPLSDTTNLAPAMAGAKLFDHIRHMMYTTGLSYGIAAILYIILGLQY
                     RGKSIDAGEIQSILTAIESIYYINPLFLLVPVITIGAVAMKIPAIPGLLLGVVLGSFC
                     MFFQGSGDIGSIIDAIHYGVAAESGHAMVDELLSGGGMDSMMWTISLIMCALTFGGVL
                     ESTGMMASIANNMLALAKKTGSLILVTILSCLFVNILCADQYLGIALPGKMFKDAYAK
                     RGLAPRNLSRALEDSGTVTSALVPWN"
     gene            complement(1591..>2359)
                     /locus_tag="PLD67_07640"
     CDS             complement(1591..>2359)
                     /locus_tag="PLD67_07640"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOT22432.1"
                     /translation="SLGGTKDSPSRNRSVLIGVIRSVDNKNAVRFELRSPNPYSNTYL
                     VIAACIQGMLDGIVSVTDAGMDCNLLEKEISKKYGEDAVYLEKFREYRSEKNVFEYYS
                     QDERDKLYGKAPTTVWENINAFASNEIKQQVLKKGDVFTDEIINAFRVSTTEKWKNEL
                     EGRIIHDNIMLLKTFVKLHSEQDHATDLDVVNWERIVYLKTKLMKDSMTKKCIFTKIK
                     NAISQGDYDIASDLQIQMNEKMSEIRTLYIGYKNNIF"
BASE COUNT          702 a          515 c          375 g          767 t
ORIGIN      
        1 attccatgga acaagggctg atgtaactgt tcctgaatct tcgagagctc gagacaagtt
       61 tctcggtgcc aaacctcttt tagcgtaagc atccttaaac attttacccg gtaaagcaat
      121 acctaagtat tgatcggcgc acaggatgtt tacaaacaag caggataata tcgttactaa
      181 aatcaatgaa cctgtcttct ttgcaagtgc aagcatattg tttgcaatgc ttgccatcat
      241 gcctgttgat tcaagaacac cgccgaaggt cagggcgcac ataatcagtg atatggtcca
      301 catcatgcta tccatacctc cgcctgacaa caactcatca accatagcat gacctgattc
      361 tgcagctact ccgtaatgga tagcatctat tatgcttcct atatctccgc tgccctggaa
      421 aaacatacag aatgatccta atacaacacc taacagaagt cccgggattg caggtatttt
      481 catggcaaca gcgccgattg taataacagg aaccaataag aataatgggt tgatataata
      541 aatgctttca attgctgtta aaatactttg aatttctcct gcatcaattg atttacccct
      601 gtattgcagc cctaatataa tgtatagaat agctgcaatt ccgtatgaaa gacctgtcgt
      661 atacatcatg tgtctgatgt ggtcaaacaa ttttgcgcca gccattgcag gagccaagtt
      721 tgttgtatct gacaagggtg acatcttgtc gccgaaatat gctcccgata ctatgcatcc
      781 tgcggccaaa gccggtgaaa ctccaagtcc ttgagctata cctataagtg caactccaac
      841 cgttcccgca gtagtccatg aacttcctgt agccaatgaa acaacgctgc acaacaacaa
      901 ggcagtaatt aagaagaagg taggcgataa tatctgcaag ccgtagtaaa ttaatgtcgg
      961 tactacaccg ccgccaatcc atgttccaat caacataccg attatggcaa gtattataat
     1021 tgcctgcata acgcttccta ttgagtcaat tatacccttt tcaacttctt cccagctata
     1081 gcctaatcca aaaactgcta tcagtgatcc taccgctgct cctaccaaaa taggaatatg
     1141 cgggtctgct cccaacacta ttattgaata cgccaatacg gctactataa caagtaccgg
     1201 gataagcgaa attcctaatg ttgcttcctt cttttctctt ggaaccttac tcattattct
     1261 cctccattat tttgttcgac ataatctcag gacaaattaa tgctaattca tataatagtc
     1321 caacccatac ccaataataa acgttatcct gacttatgtg cgattatttt ttaaccgtag
     1381 acttactatc caataagtaa gaatacgttt gttgccgtaa ttctaccata tgtttatagt
     1441 tttgtaaaca ccattttttg tataaaattt atatataagt ataaaaaatt agacaaaaac
     1501 ccccttcatt agtatgttca taaaaagtaa gggatacatt aaatagttat aatgtaaaga
     1561 gtcaccctat ttaaagtatg cccctcttac ttaaaatatg ttatttttat atccaatata
     1621 cagtgtcctt atctcagaca ttttttcatt catttgtatt tgtaaatcag aagcaatatc
     1681 atagtctccc tgagaaattg catttttaat ctttgtaaat atgcactttt tagtcatgct
     1741 gtccttcatt agttttgttt ttaaatagac aattctctcc cagtttacta catctaaatc
     1801 cgtagcatgg tcttgttcgc tgtggagttt cacaaatgtt tttaacagca tgatattatc
     1861 atgtattatt cttccttcaa gttcattttt ccacttttca gtggtggata ctctgaaagc
     1921 attaatgatt tcatcagtaa aaacatcgcc ttttttcaaa acctgctgct ttatttcgtt
     1981 agaagcaaat gcattaatat tttcccatac ggttgttgga gccttcccgt agagtttatc
     2041 tctttcatcc tgtgaatagt actcaaatac atttttttca cttctgtatt ctctgaactt
     2101 ttcaaggtag actgcatctt caccgtattt ttttgatatt tctttctcta agagattaca
     2161 gtccatgcct gcatctgtta ctgaaacaat accgtctaac atcccctgaa tacatgcagc
     2221 tataacaagg taggtgttgg aatatggatt tggtgacctt aattcaaatc ttacggcatt
     2281 tttattgtct acacttctga ttacgccgat aagaactgac cggtttctcg aaggtgaatc
     2341 ctttgtacct cctaaggaa
//
LOCUS       DAOSOW010000305         2357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_45226_length_2357_cov_2.396177, whole genome shotgun sequence.
ACCESSION   DAOSOW010000305 DAOSOW010000000
VERSION     DAOSOW010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45226_length_2357_cov_2.396177"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..304)
                     /locus_tag="PLD67_07645"
     CDS             complement(98..304)
                     /locus_tag="PLD67_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOT22433.1"
                     /translation="MKKIDTCGMSCPQPVLMTKKALEESPEGLDIIVDNNTAKGNVER
                     FLKNSGYKVIITENEDNYILEARK"
     gene            complement(317..1396)
                     /gene="yedE"
                     /locus_tag="PLD67_07650"
     CDS             complement(317..1396)
                     /gene="yedE"
                     /locus_tag="PLD67_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HOT22434.1"
                     /translation="MSDKKGIIIAGGAVGIISVLLVYFGNPANMGFCIACFLRDIAGG
                     LGLHRAEVVQYIRPEIIGLVLGAFLISVKNKEFQTKGGSSPFTRFMIGIAVMIGALIF
                     LGCPLRMVLRLAGGDLNALFGIAGFVAGIFVGVIFLNKGFNLKRSYKISKTEGYIFPA
                     VNIGLLILLIASPSFLFFSEKGPGSMAAPIIMSLTAGLIVGGLAQKTRLCMVGGTRDM
                     ILFKDMYLLSGFIAILVFSFIGNLAFGFFKLGFAEQPIAHTDGLWNFLGMAVVGWGSV
                     LLGGCPLRQLILSGEGNTDSVITVMGMLVGAAIAHNFGLASSAAGVTANGKVAVFICF
                     ALLGIVSFLNSESLVKAGNLSKASN"
     gene            1678..>2357
                     /locus_tag="PLD67_07655"
     CDS             1678..>2357
                     /locus_tag="PLD67_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT22435.1"
                     /translation="MRYVYMDNGATSFPKAPGVAESMSDYILNVGTNVNRGAYSSSFK
                     AENIIYETRELLCELFCFNKPENVIFTKNITESMNVLIKGLLKEKDHVIVSSMEHNAV
                     MRPLNSLNKVEYTKVLCSKTGVLSIEDVENSIKPNTKAIIMTHASNVCGTILDLEKVG
                     QLCKKHNLFFIIDSAQTAGSIDIDINKLNADAIGFTGHKSLLGPQGIGGFIVNDGINN
                     EMTTLIEGG"
BASE COUNT          814 a          452 c          351 g          740 t
ORIGIN      
        1 ggcacgggca taagctcgca attaatattg tttttcttac attttctatt aaatttaatg
       61 gctcctgaat gcgtaaaaaa caaaacagta tatgtcatta tttccttgcc tctaatatgt
      121 aattatcttc attttctgtt atgattacct tatagcctga attttttaag aatctttcca
      181 cattcccttt agccgtattg ttatctacta ttatatccaa tccttcagga ctttcttcca
      241 atgctttttt tgtcattaat acaggctgag gacagctcat gccgcatgta tctatttttt
      301 tcatataatc ctccccttaa ttacttgctt ttgataaatt acctgctttt actaagcttt
      361 ccgaatttaa aaatgaaaca atacctaata atgcaaaaca tataaatacg gcaacctttc
      421 cgttagcagt tactcctgct gcacttgaag ctaatccgaa attgtgtgct atagccgctc
      481 caaccagcat tcccattaca gtgataacag agtctgtatt gccttcgcct gatagtatta
      541 attgcctaag cggacatcct cctaataata ccgagcccca acctactact gccataccta
      601 aaaagttcca taggccgtca gtatgtgcaa taggttgctc agcaaatccc aatttgaaaa
      661 atccgaaagc taaatttcca ataaaactaa ataccagtat agcaataaat cccgataaaa
      721 gatacatatc tttaaaaagt atcatatcac gagtcccgcc gaccatgcac aatcttgttt
      781 tttgagctaa tccaccaact atcaggcctg ccgtcagcga cattattata ggtgcagcca
      841 ttgagccggg acctttttca ctaaagaaca aaaagctggg tgaagcaata agaagtatta
      901 aaagtcctat atttacagcc gggaaaatat agccttcggt ttttgatatt ttatagcttc
      961 tcttaagatt aaaccctttg tttaagaaaa taacccctac aaatattcct gcaacaaaac
     1021 cggcaattcc gaataaagcg tttaaatctc ctcccgcaag ccttaaaacc attcttagcg
     1081 ggcatcctaa aaatattagt gcgccaatca taactgctat gcctatcata aatcttgtaa
     1141 acggtgatga accgccttta gtttggaatt ctttattttt aacggatatt aaaaatgctc
     1201 ccagtacaag acctataatt tcaggcctta tgtattgaac gacttctgcc ctatgtaatc
     1261 ctaatccccc tgctatatct cttaaaaaac aagctataca aaagcccata tttgcaggat
     1321 tcccaaaata tacaagtagt actgatatta ttccgactgc tccgcctgct ataatgattc
     1381 cttttttatc actcattagc tctcctccta ttattaataa ttaataaatt aacaataaga
     1441 ttatatgatg gaaaattgct taaaacaata tgtatagaat tttacaatgc ttcgagaagc
     1501 aactattaat attgctgata ttaaaataga aaaccttgaa ttataagcat ttccaaaatt
     1561 tatataataa agcattaatg caacctatta aaatctttaa ttgaacaaat atttaccata
     1621 tgttaaaata atattgttta gaataaaata agaaattata tattagagga ctatactatg
     1681 agatatgtct atatggacaa tggagccacc tcttttccta aagcacccgg tgtggcggaa
     1741 agtatgtcag actatatatt aaatgtggga acaaacgtta atcgaggagc ttacagttca
     1801 tcttttaaag ctgaaaacat aatctacgaa accagggagc tcttatgtga attgttctgt
     1861 tttaataagc ctgaaaatgt aattttcact aaaaacataa ccgaaagcat gaatgtactt
     1921 atcaaaggat tattgaaaga aaaggaccat gtaatagttt cttctatgga gcacaatgct
     1981 gttatgagac ccttaaattc acttaataag gttgaatata caaaagtttt gtgcagtaaa
     2041 acaggagtat taagtataga agatgtggaa aactctatta aaccaaatac taaagccatt
     2101 ataatgaccc atgcttcaaa tgtatgcgga actattttag atttagaaaa agttggacaa
     2161 ctttgcaaaa aacataattt attcttcata atagacagcg ctcaaacagc agggtctatt
     2221 gatatagata taaataaact aaatgcggat gcaattggat ttacaggcca caaaagttta
     2281 ctgggtcctc agggaatagg cggattcatt gttaatgacg gaatcaacaa tgaaatgacc
     2341 accttgattg aaggcgg
//
LOCUS       DAOSOW010000306         2354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_45298_length_2354_cov_3.209656, whole genome shotgun sequence.
ACCESSION   DAOSOW010000306 DAOSOW010000000
VERSION     DAOSOW010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45298_length_2354_cov_3.209656"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLD67_07660"
     CDS             complement(<1..244)
                     /locus_tag="PLD67_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22436.1"
                     /translation="MNYFKSKNSIFDDFFEHRDMLIIQHMNGDINKKEYLELNYKYML
                     EKNIKPFQRIDSFEKGMYNYQYYNMMAKYHRMIAQEI"
     gene            complement(428..841)
                     /locus_tag="PLD67_07665"
     CDS             complement(428..841)
                     /locus_tag="PLD67_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22437.1"
                     /translation="MVQLVCGHSGTGKTRRMIEMANNAIDKIHGSMVFLEASNRHIFD
                     LDYRIRYINTKEFGLTNDEEFQGFVCGVIATDYDIEQVYIDGLYKIAKSGKERLVPLI
                     ERLDYLSKTFDVNFIISVNHDIEDIPEGLRDRVID"
     gene            complement(932..1846)
                     /locus_tag="PLD67_07670"
     CDS             complement(932..1846)
                     /locus_tag="PLD67_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589008.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOT22438.1"
                     /translation="MKLYNKYSDYLKLKYGEKVYKLPVNIPCTCPNRDGTLGYGGCTF
                     CADVGTGFEMLDNSLSVKEQLNKNMDYISKKYKSGKFIAYFQNYTNTYMDIERFKNYI
                     NDAIMDNIVEIAISTRPDCISDEILEFLNEIKKQNVNITIELGLQTVNYHTLKNINRG
                     HTLAEFIDAVLRIKRYNFEICTHIILNLPGDTILDTVETAKILSVLKINQVKIHSLYI
                     MENTEMGRAYKNNEITLISKDEYVERVIVFLEHLDVDITVQRLIGRAPEKDSLFVNWG
                     MSWWKINDDILYKMKSENRYQGSKNKFV"
     gene            1992..2210
                     /locus_tag="PLD67_07675"
     CDS             1992..2210
                     /locus_tag="PLD67_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
                     /protein_id="HOT22439.1"
                     /translation="MRIKEGIVPTILGTVVTATGVALKNTNLPESYRNGLIGFGLANI
                     AIGSAELVSSNWQHSRIMNRMTKMAPFK"
BASE COUNT          742 a          427 c          318 g          867 t
ORIGIN      
        1 taatttcctg tgctatcatt cggtggtatt ttgccatcat attgtaatat tgatagttgt
       61 acattccttt ttcaaagctg tcaatacgtt gaaatggttt aatgtttttt tcaagcatgt
      121 atttataatt aagctccaag tattctttct tatttatatc tccattcatg tgctgaatta
      181 tgagcatatc cctatgctca aaaaaatcat cgaatataga atttttcgat ttaaaataat
      241 tcatgtaatc actctttcta atttagtaca atgcctattc taacagaaaa atgttacatt
      301 agcaagtgct atataccata tatatggttc ttagagaaga cgtttcttac aagatatgct
      361 ttaatttgct ttaaagaaga ggtatccccg gatataataa ccgccggtca aaaaccggcg
      421 gcataaatta atctataact ctatctctca atccttctgg aatatcttca atatcatggt
      481 ttacacttat aataaaatta acatcaaatg tttttgataa gtaatctagt ctttctatta
      541 atggtaccaa tctttccttg cctgacttag ctattttgta taatccgtct atgtaaactt
      601 gctctatgtc gtaatcagta gcgattactc cacatacaaa accttgaaac tcttcatcat
      661 tggttaatcc gaattctttt gtgttaatat accttatcct gtaatctaaa tcgaatatgt
      721 gtctgttgct tgcttccaaa aaaaccatac tgccgtgaat tttgtcaata gcgttgtttg
      781 ccatttcaat catccttctt gtcttaccgg tgccgctatg accgcaaacc aattgaacca
      841 taataatcac actcctattc ataattttat aatagtaaag acacttatat tgtaccacat
      901 cttgtaattg tttacaccat tttatttaca attaaacaaa tttattttta ctgccttgat
      961 acctgttttc agacttcatt ttataaagaa tatcatcatt tattttccac cagctcatac
     1021 cccagtttac aaaaagagaa tctttctcgg gtgcccttcc tatcaatctt tgtactgtaa
     1081 tatccacatc taaatgctcc agaaaaacaa ttactctttc tacatattca tcctttgaaa
     1141 tgagtgttat ttcattgttt ttatatgctc ttcccatttc ggtattttcc attatataaa
     1201 gagaatgaat tttaacctgg ttaattttta aaacagataa tatttttgcc gtttcaaccg
     1261 tgtccaaaat ggtatctccg ggtaaattca atataatatg tgtacatatt tcaaagttat
     1321 acctcttaat ccttaatacg gcatcaataa attccgctaa ggtatgacct ctgttaatat
     1381 ttttcaaagt atgatagttt actgtctgaa gtcctaattc gattgttata tttacatttt
     1441 gtttttttat ttcgttaagg aactcaagta tttcatcaga tatgcagtca ggtctggtgg
     1501 atatggctat ttccactata ttatccatta ttgcatcatt tatataattc ttaaaccttt
     1561 ctatatccat atatgtattc gtataatttt gaaagtaggc aataaatttc ccactcttat
     1621 atttttttga aatatagtcc atatttttat ttaactgttc ttttacgctt aaagaattat
     1681 caagcatttc aaaaccggtt cccacgtcgg cacaaaatgt acaacctccg taacccagag
     1741 ttccgtctct gttaggacag gtacatggaa tatttacggg aagtttatat actttttcgc
     1801 catactttag ttttaaataa tcagaatact tattatacaa tttcatattt ttcccttttt
     1861 ataataattt ttctttaatc attataatta agttaataga ttaaatcaat taaattacca
     1921 gtaattaata aaattatttt acgattaaat gtaaataata ttaatacaaa tttaaataaa
     1981 ggaggcttca tatgcgtata aaagaaggta tcgtcccgac tattttgggt acagttgtta
     2041 ctgcaacagg agttgcttta aaaaacacaa acctgccgga atcttacaga aacggattaa
     2101 tcggatttgg attagcgaat attgctatag gttcagctga attagtttca agcaattggc
     2161 agcacagccg cataatgaac agaatgacaa aaatggcacc cttcaagtaa gcaggtgtcc
     2221 caaatctttg atttggtgtc agtcgcttat cctgagcaca agcgaaggat ctcagatgcc
     2281 ccaaatctgt gatttggcgg cagtcgctta tcctgagcac aagcgaagga tctcagatgc
     2341 ccaaaaaaac aaaa
//
LOCUS       DAOSOW010000307         2348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_45441_length_2348_cov_3.637157, whole genome shotgun sequence.
ACCESSION   DAOSOW010000307 DAOSOW010000000
VERSION     DAOSOW010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45441_length_2348_cov_3.637157"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="PLD67_07680"
     CDS             <1..894
                     /locus_tag="PLD67_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003491733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOT22440.1"
                     /translation="LEEIEDGAWINLVNPTKEELDFIENKLSIEGDLLRAALDEEESA
                     RLETEEGQVLILVDTPYIEKEDEHIIYETLPLGIIITDKNIITVCLKNSIVLDQFVKN
                     SIRTFYTYKKYRFLYQILYKNAQRYLLYLRQIDKMSNYVEEKLHQSMKNKELIQLLDL
                     SKSLVYINTSLKANQIVLEKILRINIIKKYTEDEDLLEDVIIENKQAIEMATIYSGIL
                     SGTMDAFASLISNNLNIVMKLLTSITILMAIPTMFASFYGMNVANIPLANSPFGFWII
                     IGISILTSVFAGFILAKKDLF"
     gene            1217..2086
                     /gene="ispE"
                     /locus_tag="PLD67_07685"
     CDS             1217..2086
                     /gene="ispE"
                     /locus_tag="PLD67_07685"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOT22441.1"
                     /translation="MLRLNSYAKINLFLDIKGKLQNGYHIIKTVMQSIDIYDEITLIP
                     LNDNKIIIECSDKSIPINEKNTCYKAVLLLKKAYGINSGIQIYINKMIPAGAGMAGGS
                     SNAAAVIKGLNKMWKLNLSEQEMIDIGAQIGADVPFCLVGGTCLAEGIGDKVTDLNDF
                     QWDNILLVKPEFSISTAFVYKNLDTNYYNSNCEEYILKYITSYDYEKAAKCVANTLEK
                     VVEKYHPEINYIKKLMIDNGAISSIMTGSGSAVFALFKDNDSLNKAYEIAKKIYPSTF
                     KSKTSKYGVKFID"
     gene            2131..>2348
                     /locus_tag="PLD67_07690"
     CDS             2131..>2348
                     /locus_tag="PLD67_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22442.1"
                     /translation="MKKILIIILSIALITGCTNNSADIRENEVQEAVNETQETNDEAK
                     ANEPVMAPDFELESMDGSIIKLSETRVK"
BASE COUNT          864 a          334 c          409 g          741 t
ORIGIN      
        1 ttagaagaaa ttgaagatgg tgcttggatt aacttggtta atcctacaaa agaggaatta
       61 gactttattg aaaataagct gtctattgaa ggagatcttt taagagcagc tttggacgaa
      121 gaggaaagtg cccgtttaga aacggaagaa ggtcaagttt taatattggt tgatacgccc
      181 tatattgaaa aagaagatga acatattatt tatgaaacac tccctcttgg aattataata
      241 accgataaaa atataataac agtatgttta aaaaattcaa ttgtacttga ccaatttgtg
      301 aaaaacagca ttcgtacttt ctacacttat aaaaaatacc gttttttata tcaaatacta
      361 tacaagaatg ctcaaagata tttactttat ttaaggcaaa ttgacaaaat gagcaattat
      421 gtagaggaga agctgcatca atcaatgaaa aacaaggagc ttatacagct cttggactta
      481 tcaaagtctt tggtatatat taatacttct cttaaagcaa accaaatagt actggaaaaa
      541 atccttagaa tcaatataat aaaaaaatat accgaggatg aggatttatt ggaagatgta
      601 attattgaaa ataagcaagc cattgaaatg gccacaattt acagcgggat tttaagcggt
      661 acaatggatg catttgcatc tcttatatca aataatttaa atattgttat gaagctctta
      721 acatccataa caatattaat ggctataccg accatgtttg caagctttta tggaatgaac
      781 gttgctaata tccccctcgc aaattcgcct tttggtttct ggataataat tggtatttca
      841 atactgacat cagtatttgc aggatttata cttgcaaaaa aagatttgtt ctaaggtatg
      901 gggacacacc ttcaaagtcg ggtcactcta tgtgggataa cttttgaagg aatgtcccca
      961 atttgagggg gacacacaaa aaatgaataa cacccgtttc tcatcagtta cacaccagag
     1021 gacgggtttt attcattcat agtttcaatt tccgttttcg ggggtaccac cctctttctt
     1081 tatttgcttc attaaattta tacccaataa tttaaatttt aatcataaaa aactattaaa
     1141 tttataaata attgtatttt ttgttgttga aaaataaatt atgttatata atgttgaaaa
     1201 aaattgagag atggctatgt tgagattaaa ctcatacgca aaaattaatt tatttttaga
     1261 tattaaggga aaacttcaaa acggttatca tataattaag actgttatgc agtctatcga
     1321 tatttatgat gaaataaccc ttattcccct taatgataat aaaattatta ttgaatgctc
     1381 cgataaatct attccgataa atgaaaagaa tacctgttat aaagctgtgc ttttattaaa
     1441 gaaagcttac ggtataaatt caggcataca aatatatatc aacaaaatga ttcctgcggg
     1501 agcgggcatg gcaggaggca gttccaatgc agctgcagta ataaaaggct taaataaaat
     1561 gtggaagctt aatttatcgg aacaagaaat gatagacatt ggagcacaaa tcggtgctga
     1621 tgtgcctttt tgcttagtag gaggtacttg tctggcagaa gggattggtg acaaagttac
     1681 tgatttaaat gatttccaat gggataatat tttattggtg aagccggaat tttcaatttc
     1741 gactgcattt gtttataaaa accttgatac caattactat aattctaatt gtgaagaata
     1801 catactgaaa tatattacat catatgatta tgaaaaagct gctaaatgtg ttgcaaatac
     1861 attagaaaaa gttgtggaaa agtatcatcc cgaaatcaat tacattaaga aattaatgat
     1921 tgacaatggt gcaatatctt ccataatgac cggttcaggc tctgctgtat ttgctctttt
     1981 taaagacaat gattccctga ataaggcata cgaaattgct aaaaaaatct atccttctac
     2041 atttaagtcg aaaacatcta agtatggagt taaatttata gattagacaa attcgtatct
     2101 gggtatcagt taattaataa ggagattacc atgaaaaaaa tactgattat tattttatca
     2161 atagcattga taaccggatg tacgaataat tcagctgata ttcgtgaaaa tgaggtgcag
     2221 gaagctgtca atgaaactca agaaaccaat gatgaagcaa aagcaaatga gcctgtaatg
     2281 gcaccggatt ttgaacttga gtctatggac ggctcaataa taaaactgag tgaaaccaga
     2341 gttaagaa
//
LOCUS       DAOSOW010000308         2348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_45443_length_2348_cov_3.274749, whole genome shotgun sequence.
ACCESSION   DAOSOW010000308 DAOSOW010000000
VERSION     DAOSOW010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45443_length_2348_cov_3.274749"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(363..785)
                     /locus_tag="PLD67_07695"
     CDS             complement(363..785)
                     /locus_tag="PLD67_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOT22443.1"
                     /translation="MEYKRFDNTILARIDKGEEILEKLMEICEKEHVKLANVNALGAI
                     GEFTIGLFDTSEKKYYSANYTGDYEIVSLTGSISTMDGKLYNHIHLSAGDKDNKVYGG
                     HLNKAVVSATCEMFIHIVNGKVNRKFDEEVGLNLLDFN"
     gene            complement(802..1830)
                     /locus_tag="PLD67_07700"
     CDS             complement(802..1830)
                     /locus_tag="PLD67_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22444.1"
                     /translation="MLLFRIGPRHLFLRTENIKEVSNFLVTKLKGEVVEFWEGFVKAS
                     EDSTLCFITGIDHEKTYVEDAKEIVLVNDASSPILCSIISNPVSELLQKVDMGPASIV
                     MRIPPGNADKIIEKTKEIFDGQEVDWIEGIGIGEKDDTIISFTRQELSRAVTDFLDTK
                     LLISKPVNEVKDRLRLEGLRLITHSLDDSQWYELRINIYDSYGNYQQHYERLMFILSK
                     LEIGMVLGESWTKDFAVILYSVLTYQVRLFTFHTPIEIKKILMALEYTADGKRLVDFD
                     LYYKNKKVYWYEVNKNRGKGIEKSDEAKNQRQNLYKKLKPEDIIKLEQMEKDSLDALK
                     KVLSGSEN"
     gene            complement(1841..>2348)
                     /locus_tag="PLD67_07705"
     CDS             complement(1841..>2348)
                     /locus_tag="PLD67_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOT22445.1"
                     /translation="PSEIYNNIFKIIDIIKPQYIVHTGDLADNIKLKIQKDRINSYCK
                     GVKELVEGLEKNDSEVYYLMGNHDDYEVVSQFTKKGEILEEGLLPIDNLTITVGHYYK
                     EHSFKSDFNLYGHSFEPGHYKKDGTIGLNGVLNINIIDLSNKEVFHLNYPNGTNHLRC
                     MNTKKIGL"
BASE COUNT          684 a          484 c          315 g          865 t
ORIGIN      
        1 tagaataaaa agccactgct tagtagtaaa attttcgtca aattcatcgc ctaatgtctg
       61 cagcctgtta gataaggtaa aaattgttgc aaaaatatat gctttctgtt taagttcatt
      121 catcaatttt cctccagtat atgcaacatg ttacctgtat tgtatatggc ttgcttttct
      181 ttgtcaagaa aaaaagacaa agtataaaac tttgtccgag ttgttagggt atcccattat
      241 gtcatcctga gcttaagcga aggatctcag gttttataag atgagattct tcgagctaaa
      301 gctctcagaa tgacacgtag cttattcata gcaatgacaa tcgttatata ctttgtctaa
      361 ctctaattga aatcaagcag gttaagtccg acttcttcgt cgaattttct gttaacctta
      421 ccgtttacta tatgtatgaa catttcacat gtggcgctta caaccgcctt attcaaatga
      481 cctccataaa ctttattgtc cttgtcaccg gcacttaaat ggatgtggtt atatagttta
      541 ccatccattg tagaaatgct ccccgtcaat gatactattt cataatctcc tgtataattt
      601 gcagaatagt attttttttc acttgtatca aacaatccaa tcgtaaattc tccaatcgct
      661 cccaaagcat ttacatttgc cagttttaca tgttcctttt cgcaaatttc cattaacttc
      721 tcaagtattt cttcaccttt atcaattctt gccaatattg tattatcaaa tcttttatat
      781 tccatttttt actctcttcc tttaattttc agagccggat agaacctttt tcaaggcatc
      841 caagctgtcc ttttccattt gttcaagttt tattatatct tcaggcttaa gctttttata
      901 caagttttgt ctttgatttt tagcttcatc tgacttttca ataccttttc ctctattctt
      961 gttaacctcg taccagtaaa cttttttatt tttatagtac aaatcaaagt cgactaacct
     1021 cttgccatca gctgtatatt ccaatgccat taaaattttc ttaatttcta ttggagtatg
     1081 aaaagtaaac aatctcacct ggtaggtcaa taccgaataa agtatcacgg caaagtcctt
     1141 tgtccagctt tctcccaata ccatgccgat ttcaagctta gatagtataa acataagtct
     1201 ttcatagtgt tgttggtaat ttccataaga atcataaata tttattctaa gctcatacca
     1261 ctggctgtca tccaagctat gagttattaa cctcagtccc tctaatcgca atctatcctt
     1321 aacctcatta acaggctttg aaatcaataa ttttgtatct aaaaaatctg taaccgctct
     1381 gctgagttcc tgtcttgtaa aagaaatgat agtatcatcc ttttctccaa ttccaatccc
     1441 ttctatccaa tcaacttctt gaccgtcaaa aatctccttg gttttttcaa ttattttgtc
     1501 tgcattaccg ggtggtatgc gcataactat tgaagcaggt cccatgtcaa ccttttgcag
     1561 caactctgaa acaggattgc tgattattga gcagagtatt ggtgaagatg catcgtttac
     1621 aagtacaatt tcctttgcat cctccacata ggttttttca tggtcaatac cggttataaa
     1681 acataatgta ctgtcctccg atgcttttac aaatccctcc caaaactcta caacttcccc
     1741 cttaagtttt gtaactaaaa aatttgaaac ttctttgata ttttcagttc ttagaaacaa
     1801 gtgccttggt ccgattctaa acagcagcat aacatacccc ctataagccg atttttttag
     1861 tattcatgca tctcaaatga tttgtaccat taggataatt taaatgaaat acctctttat
     1921 ttgataaatc tattatattt atatttaata ccccgtttaa gccgattgta ccatcttttt
     1981 tgtaatgtcc cggctcaaaa ctatgaccgt aaaggttaaa atcagactta aatgaatgct
     2041 ccttgtagta atgccccact gtaatggtta aattatcaat aggtaaaagt ccctcctcta
     2101 aaatttcacc cttcttggta aattggctga caacttcata atcatcatgg ttgcccatca
     2161 aatagtatac ctctgagtca tttttctcca aaccctcgac gagttcttta actcctttac
     2221 aatagctgtt gattctgtct ttttgaattt ttaacttaat attatcggct aagtcacctg
     2281 tatgcacaat atattgcggt tttataatat cgattatttt aaaaatatta ttataaattt
     2341 ctgaaggg
//
LOCUS       DAOSOW010000309         2345 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_45524_length_2345_cov_3.120087, whole genome shotgun sequence.
ACCESSION   DAOSOW010000309 DAOSOW010000000
VERSION     DAOSOW010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45524_length_2345_cov_3.120087"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="PLD67_07710"
     CDS             complement(<1..925)
                     /locus_tag="PLD67_07710"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007708433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOT22446.1"
                     /translation="MIIKIKLKSLLIFFAIIFVVLAIYLFMNKTQSVSADEEKDFIKW
                     VDFRVSYEAMDKALRADINSLDEEVKLDWIEILAYLGTRYGGDFFSRYKAKDMDKLIN
                     RLKSGETIESMTKDLKHYNYYYEAYKAVLEGFVGPFEIQVKDEDTGEKVWVKKYGLKA
                     FSPIAGGYHYGHYDDFGNSRSYGFRRVHLGNDLVGSIGTPIIAVESGRIEALGWNQYG
                     GWRIGIRSLDNKRYYYYAHLRKDHPYVKTLKEGDIVLAGDVIGYLGMTGYSRKENVNN
                     IRTPHLHFGMQLVFDESQRKHWQKYGLTYTEL"
     gene            1063..1851
                     /locus_tag="PLD67_07715"
     CDS             1063..1851
                     /locus_tag="PLD67_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014968955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT22447.1"
                     /translation="MKLKFCSLYSGSSGNCQYIKTKNTTIIVDAGLSGKRIQQEIANI
                     GEDPNKIDAIFITHEHIDHIQGAGIMSRRLNIPIYANEETWEAMKSLIGDIKSCNIKI
                     LDESTQIGDLTIKSFDISHDAAHPVGFNIFYENKKISLVTDTGCFNGTITNSIMDSDL
                     LLVESNHDEDLVIIGPYPWPLKRRVLGEFGHMSNEAAGNLIKKVIKKGTEIILLGHLS
                     KENNFPELAYKTVESILLEDSIDVNPGICLDLTYRDKSSKVYEI"
     gene            1859..2020
                     /locus_tag="PLD67_07720"
     CDS             1859..2020
                     /locus_tag="PLD67_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxH/CxxC protein"
                     /protein_id="HOT22448.1"
                     /translation="MKEMYEEIFACMEHVEQAIDDYVNSKEAAPQLIKTDKEEKCNYC
                     KKNAEYIIK"
     gene            2111..>2345
                     /gene="rpsF"
                     /locus_tag="PLD67_07725"
     CDS             2111..>2345
                     /gene="rpsF"
                     /locus_tag="PLD67_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOT22449.1"
                     /translation="MRKYEGAFILLSNLEEDVRNAEIEKIQSIITERQGTIEKVNEWG
                     QRRLAYEINKKREGYYIFIEFTAGADAVDEVDRI"
BASE COUNT          839 a          392 c          411 g          703 t
ORIGIN      
        1 acaattctgt atacgtcaat ccatatttct gccaatgctt cctttgagac tcatcaaaaa
       61 ctaactgcat tccaaaatga agatgaggag tccttatgtt gttgacattt tcttttcgcg
      121 aataaccggt catccctaaa tatccgatta catctcctgc caatacgata tctccttcct
      181 taagggtctt gacataaggg tgatccttac gcaagtgagc ataatagtaa taccttttgt
      241 tatccaggct gcgtattcct attcgccatc ctccgtattg attccagcct aaggcttcaa
      301 ttcgtcctga ttcaactgcg ataatgggag tacctatcga accaaccaag tcatttccca
      361 aatgaactct tcggaaacca tagctgcggg agttcccgaa atcatcataa tgtccgtaat
      421 ggtatccacc tgctattggt gaaaatgctt ttaatccgta ttttttaacc caaacctttt
      481 ctccggtgtc ttcatctttt acctgtattt caaaaggtcc tacaaatcct tccagcacag
      541 ccttgtatgc ctcatagtag taattgtaat gtttgagatc cttggtcata ctttcaattg
      601 tttcaccgct ttttagtctg tttattaatt tatccatatc ttttgcttta tatcgagaaa
      661 aaaaatcacc tccatatctt gttccaagat aggctagtat ttcaatccaa tctaatttaa
      721 cttcttcatc aagactattt atatcagccc ttaaagcttt atccatggct tcataggaaa
      781 ccctgaaatc tacccacttt ataaaatcct tttcttcatc tgcagaaaca gattgagtct
      841 tattcataaa taaataaatt gccaatacaa caaatataat agcaaaaaat atcaataatg
      901 atttcaattt aattttaata atcataatta cctcaaatat tataatatag taaatattat
      961 gcaaataatg agtggataga accttagaaa gtatttgtaa aactaatgct tttatgatag
     1021 tataaattga ttgattgtag ttcatgtaca ccgggaggac gaatgaaact gaaattctgt
     1081 tcgctatata gcggaagcag tggaaactgc caatatatca agacaaaaaa tacaactatt
     1141 attgttgatg caggattaag cggaaaaaga attcagcagg aaattgcaaa tataggggaa
     1201 gatccgaata agatagatgc catattcata acgcatgaac atattgacca tattcaaggt
     1261 gcaggaataa tgtcaaggcg attaaatatt cccatttatg ccaatgaaga aacatgggaa
     1321 gcaatgaaat cattaattgg tgatataaaa tcatgcaata ttaaaatttt ggatgaaagc
     1381 acacagatag gggatttaac aatcaagtca tttgacatat cccacgatgc agcacatccg
     1441 gtaggtttca atatatttta tgaaaataaa aaaataagct tagttacaga tacaggttgt
     1501 ttcaatggta caattacaaa cagcattatg gattcagatt tattgctggt ggaatcaaat
     1561 catgatgaag acttagtaat cataggtcca tatccatggc cgcttaaaag aagagtgttg
     1621 ggtgaattcg gacatatgtc aaatgaagca gcaggcaact taataaaaaa ggttataaaa
     1681 aaaggaacag aaataatctt gcttggacat ctgagcaagg aaaataactt tcccgagctt
     1741 gcctataaga cggtagaatc aatattattg gaagattcca tcgatgtaaa tccgggtatc
     1801 tgccttgatt taacataccg ggataaatca agcaaagtat atgaaatata ggtgaatcat
     1861 gaaagaaatg tatgaagaga tatttgcatg catggagcat gtggagcagg caatagatga
     1921 ttatgtgaat tctaaggaag ctgctcctca attgattaag acagataaag aagaaaaatg
     1981 caactattgt aaaaaaaatg ccgaatacat aataaaataa tacttgaatt aggttttttc
     2041 aagtgctata atgtataagc tatcctgccc ttggcgaaca agggccaaaa gtccaaagga
     2101 ggtgactcaa atgagaaaat atgaaggtgc ttttatctta ttatctaact tagaggaaga
     2161 tgtaagaaat gcagaaattg aaaaaattca aagcataata accgagagac agggaacaat
     2221 tgaaaaggtt aatgagtggg gtcaaagaag acttgcttat gaaatcaaca aaaaaagaga
     2281 aggttattat atttttattg aatttactgc aggagcagat gctgttgatg aagtagacag
     2341 aattt
//
LOCUS       DAOSOW010000310         2332 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_45866_length_2332_cov_2.377690, whole genome shotgun sequence.
ACCESSION   DAOSOW010000310 DAOSOW010000000
VERSION     DAOSOW010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45866_length_2332_cov_2.377690"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            14..988
                     /locus_tag="PLD67_07730"
     CDS             14..988
                     /locus_tag="PLD67_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006313347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT22450.1"
                     /translation="MSEYLITVNNLKKYFPIKKGLVKRTSGNVKAVDGVSFNIQRGQI
                     LGIVGESGCGKSTMGRTILRWLPPTGGEVFYEGKNVFNMSKREMNKMRTKMQIIFQDP
                     YSSLNPRLPVYDIVGEAMLQHGIVKNKKEMKEKVIEIIGKCGLSPEQADRYPHQFSGG
                     QRQRICIARALAVNPDFMVCDEAVSALDVSIQSQIINLLKDAQEDYGLTYMFISHDLS
                     IVKFISDEIAVMYLGQIIEKGGKKQIFSNPLHPYTEALLSVVPSLKQNKRRIVLNGDI
                     PSLVNPPKGCRFYTRCPYAKDICKEEIPEYREINTGHFAMCHKVNGVF"
     gene            988..1221
                     /locus_tag="PLD67_07735"
     CDS             988..1221
                     /locus_tag="PLD67_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22451.1"
                     /translation="MFFRRNRVEEDLERIREANLPDREEIKMNEKQSDDLGLEKGDIL
                     AMVLAVISLILPYVIAFLGAIGVVIFFMYLFFS"
     gene            complement(1308..1508)
                     /locus_tag="PLD67_07740"
     CDS             complement(1308..1508)
                     /locus_tag="PLD67_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018758750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HOT22452.1"
                     /translation="MAQGTVKWFNSEKGFGFITKEDGNDVFVHFSAIQGDGFKTLEEG
                     QRVSFDVTQGNRGDQAANVQRI"
     gene            complement(1777..>2332)
                     /locus_tag="PLD67_07745"
     CDS             complement(1777..>2332)
                     /locus_tag="PLD67_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOT22453.1"
                     /translation="NDKLLEITDKKTTMLEAFAMADEVLRQGIQGISDLIAIPAIMNL
                     DFADVETIMSGQGIAHMGIGMASGENRALNAVKQAIQSPLLETSINGARGVLVNITGG
                     PSMGIHEVNEAAKLIEEAADPEANLIYGANIDESAGDTIKITVIATGFADGAKKSDGE
                     PSEKSEKKPENPFDFTIPSFLNKK"
BASE COUNT          725 a          428 c          445 g          734 t
ORIGIN      
        1 taggaagaag tagatgagtg aatatttgat taccgtaaat aatttaaaaa agtattttcc
       61 cataaaaaaa ggacttgtta agcgtacaag cggaaatgta aaagccgtgg acggagtatc
      121 cttcaatatt cagaggggac agattcttgg aatagtagga gaatcgggct gcggaaaatc
      181 tactatggga agaaccatct taagatggtt gccaccgaca ggaggtgaag tcttttatga
      241 aggtaaaaat gtttttaaca tgagtaaaag agaaatgaac aaaatgcgaa ccaaaatgca
      301 gattatattt caagacccat attcttcatt gaatccaagg cttcctgttt atgatatagt
      361 gggcgaagca atgctacagc atggtattgt aaaaaataaa aaggaaatga aggaaaaggt
      421 aattgaaata atcggtaagt gcgggctttc cccggaacag gcagaccgtt acccccatca
      481 gttttccgga gggcaacgtc agcgtatttg cattgcaagg gccttagccg ttaatcctga
      541 ttttatggtc tgcgacgaag cagtttctgc attggatgtt tctatacagt cacaaataat
      601 aaatctgtta aaagatgcac aggaagatta cgggcttacc tatatgttta tatctcatga
      661 cctgtcgata gtaaaattta tcagtgatga gatagcagtt atgtaccttg gtcaaataat
      721 tgaaaagggc ggaaaaaaac aaatattttc aaaccccctc cacccgtata cggaagcatt
      781 attgtcagtt gtcccttcac ttaaacaaaa taaaagaagg atagttttga atggagatat
      841 tccatcgctg gtaaatccgc caaaaggatg tcgtttctat acacgatgtc cctatgcaaa
      901 agatatatgc aaggaagaaa tacctgaata cagggaaata aataccggac attttgcaat
      961 gtgtcataag gtaaatggag tattttaatg ttttttagaa ggaatagagt agaggaagat
     1021 ttggaaagaa ttcgggaagc taaccttccc gacagagaag aaataaaaat gaacgagaag
     1081 caatcagatg accttggtct ggaaaaaggc gatattcttg ccatggtatt ggcagtaata
     1141 tctctgatac tgccctatgt aattgctttt ttgggggcta tcggagttgt gatatttttt
     1201 atgtatttgt tttttagttg aaaaatatac gacaataagc tatagcagag gaatatggaa
     1261 gtatataaaa aatcgggatt tctcccgatt ttttctttta cagctgctta tattctttga
     1321 acgttagccg cttggtctcc tctgtttcct tgagtaacat caaagcttac tctttgacct
     1381 tcttcaagag ttttgaatcc gtctccctga atagctgaaa aatgtacgaa aacgtcattt
     1441 ccatcttctt ttgtgataaa tccaaatcct ttttctgagt taaaccattt tactgtaccc
     1501 tgtgccatga gtacctccta aaaaattatt atattccata gtaaaaaaat ttcacacatt
     1561 tttacacact atatacggca cgccacatat aatatctaaa aacatgtgaa atccataaat
     1621 acatctgtaa tacattttaa gactaacaca tcaagcttta tatgtcaaca attatttttt
     1681 tggaaaatca aggaacaatt ggatatttcg ttttgggacg gttcttttat cggtgcctgg
     1741 caccacaaat ggtgcccggc accgataccc aaaattttat tttttattca agaagcttgg
     1801 tattgtaaaa tcgaacgggt tttccggttt cttttcgctt ttctcactcg gttccccatc
     1861 ggattttttt gctccgtctg caaaacctgt cgcaataaca gtaattttaa tcgtatctcc
     1921 ggcagattcg tcaatatttg ctccatatat taagtttgct tccgggtcag cagcttcttc
     1981 gattaatttt gctgcttcgt taacttcatg gattcccatg cttggaccac ccgtaatatt
     2041 taccaacaca cctcttgctc cattgatgga tgtttctaaa agaggacttt gtattgcctg
     2101 tttaactgca tttaatgctc tgttttctcc tgaagccatt ccgattccca tgtgagcaat
     2161 accttgaccg gacataattg tttcaacgtc ggcaaaatca aggttcatta tggcagggat
     2221 tgctatcaaa tcagatatac cctgtatacc ttgtctcaat acttcatctg ccatggcaaa
     2281 agcttccagc atggttgttt tcttgtctgt tatctccaaa agtttatcgt tc
//
LOCUS       DAOSOW010000311         2329 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_45947_length_2329_cov_2.395778, whole genome shotgun sequence.
ACCESSION   DAOSOW010000311 DAOSOW010000000
VERSION     DAOSOW010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45947_length_2329_cov_2.395778"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PLD67_07750"
     CDS             <1..710
                     /locus_tag="PLD67_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit C"
                     /protein_id="HOT22454.1"
                     /translation="NKPDAIETLKQAVGDEEGIEVVSMIAKYPQGDEKRLINAVTGRV
                     VPSGGLPMEVGCVVENVGTVATIGNVIKTGMPCIQRVVTVTGSAVENPKNLYVRIGTL
                     FKDCIEMCGGYKEEPGKIISGGPMMGIAQYTDEVPVIKGTSGILVLNKKEANVPEHSN
                     CILCGKCVEACPVYLMPYKIARFSLLRNFDGADEYHATDCIECGACSYVCPAKRPLKE
                     SISVAKKEILTRRKKAK"
     gene            723..1673
                     /locus_tag="PLD67_07755"
     CDS             723..1673
                     /locus_tag="PLD67_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOT22455.1"
                     /translation="MEIKLIGSSSPHIRSDETTQKLMLDVIIALIPALAASIYYFGYK
                     AMILVLASVISAVATEYFCQKFMNRPITVTDLSAVVTGILLAFNLPAEAPWWIAVIGS
                     VFAIGIVKQIFGGIGFNFLNPALGARAFLMASWPHHLSGGFIDPAIDAVTSATPLSII
                     KGAASGQLPSLWDMLIGNIPGVIGETSAILLLAGGIYLIYTGTIKWIIPAFYIGTVAA
                     IALLFDAGNLSYHILGGGLMLGAFFMATDYATSPITDKGKVIYAVGAGMLTMFIRKMG
                     GYPEGVSYSILLMNILTPMIDKYVKPRVFGVATGGKKNEK"
     gene            1663..2235
                     /locus_tag="PLD67_07760"
     CDS             1663..2235
                     /locus_tag="PLD67_07760"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HOT22456.1"
                     /translation="MKNNFVKLAGVLLIIGAVSAGVLAWLNDVTSDRIAQNEAMASMD
                     PAILEAVMPGSVMFNDYEDTALVDTIKADNNKFINLLTAVDESGNELGKVIRTWSTVK
                     GFNGDLELYVGFTPDGQISGTSVISHAETEGVGSKVTLLAFTEQFIGKDASGEISEGD
                     YDAVTGATYSSKSFLSAVNNALNIYAEYVK"
BASE COUNT          781 a          350 c          516 g          682 t
ORIGIN      
        1 ataacaagcc tgatgcaatt gaaacattga agcaagctgt tggtgatgaa gaaggtattg
       61 aagttgtatc gatgatagct aagtaccctc aaggtgatga aaaaagatta attaatgcag
      121 ttaccggaag agttgtacct tcaggaggac ttccaatgga agttggatgt gttgtagaaa
      181 atgtcggtac agttgctaca ataggcaatg taataaaaac aggtatgccc tgcattcaaa
      241 gagttgtaac cgtaacagga agtgctgttg aaaaccctaa aaacctatac gtaagaatag
      301 gaactctgtt taaggattgt attgaaatgt gcggcggtta caaagaagag ccgggaaaaa
      361 ttatcagcgg cggaccaatg atgggaattg cccaatatac tgacgaagtc cctgttataa
      421 aaggaacatc gggaatattg gttttaaata aaaaagaagc aaatgtaccg gagcattcaa
      481 attgtatttt atgcggcaaa tgtgttgagg cttgtcctgt atacctaatg ccttacaaaa
      541 tagcaagatt ttcattattg agaaatttcg acggtgctga tgaatatcat gcaactgatt
      601 gtatagaatg cggtgcttgt tcgtatgtat gtcctgcaaa gagaccgctt aaagaatcga
      661 taagtgttgc aaaaaaagaa attttaacca gacgcaaaaa ggctaaatag gaggaaatat
      721 tgatggaaat taaattgatt ggttcatcgt cacctcatat tagatccgat gaaacaacac
      781 aaaaattaat gcttgatgtg ataattgcat taattccagc attggcagca agcatatatt
      841 attttggata taaagcaatg atattggtat tagcatctgt aatttctgca gttgctaccg
      901 aatatttttg tcaaaaattt atgaacagac caataacagt taccgattta agtgcggttg
      961 taacaggaat actcttagca tttaatttac ccgcagaagc accatggtgg atagcagtta
     1021 tcggctctgt atttgcaata ggaatagtaa aacagatatt tggcggaata gggtttaact
     1081 ttttaaatcc ggcattggga gcaagagcat tcttaatggc atcatggcct catcatctgt
     1141 caggaggatt tattgacccg gcaattgatg cagttacttc tgcgacacct ctttctatta
     1201 tcaagggtgc agcatccgga caactgccgt cattgtggga tatgttaata ggaaatatac
     1261 cgggagttat cggagaaact tcggcaatac tattattagc cggtggaata tatttaattt
     1321 acaccggaac aatcaaatgg ataattccgg cgttttatat aggtacagtt gcagcaatag
     1381 cattactttt tgatgctggc aatttatcat accacatctt aggtggaggt ttgatgttgg
     1441 gagcattttt catggctacc gattatgcaa caagccccat taccgataag ggaaaagtta
     1501 tatatgcagt tggagccgga atgcttacaa tgtttataag aaaaatggga ggatatcccg
     1561 aaggagtttc ttattcaata cttctgatga atattttgac tccaatgata gataaatatg
     1621 ttaagcctcg cgtattcgga gttgcaacag gaggtaaaaa aaatgaaaaa taattttgta
     1681 aaacttgcag gagtattgct tattataggt gcggtatcag caggagtatt agcatggctg
     1741 aatgacgtaa caagtgatag aattgcacaa aatgaagcaa tggcaagtat ggacccggct
     1801 atattggaag cagttatgcc cggatctgta atgtttaatg attatgaaga tactgcatta
     1861 gttgatacaa ttaaagctga taataacaaa tttattaatc ttcttacagc tgtcgatgaa
     1921 tcaggtaacg aactggggaa agttataaga acatggagta ctgttaaagg tttcaacgga
     1981 gacttggaat tatatgttgg tttcacccct gatggacaaa tatccggaac aagtgtaata
     2041 tcacatgctg aaactgaagg agtaggctct aaagttactc tacttgcatt tacagaacag
     2101 tttattggca aggatgcaag cggcgaaatt tctgaaggtg attatgatgc tgttacaggc
     2161 gctacgtatt cttctaaatc attcttaagt gccgtaaaca atgctttaaa tatttatgcg
     2221 gaatatgtga aataggagga taacatgaat agatttgaaa tatttaaaaa tggagtaaca
     2281 acagaaaatc ctattttagt tcaacaggtt ggtatgtgtg ctaccttgg
//
LOCUS       DAOSOW010000312         2325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_46057_length_2325_cov_2.509251, whole genome shotgun sequence.
ACCESSION   DAOSOW010000312 DAOSOW010000000
VERSION     DAOSOW010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46057_length_2325_cov_2.509251"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(499..1944)
                     /locus_tag="PLD67_07765"
     CDS             complement(499..1944)
                     /locus_tag="PLD67_07765"
                     /inference="COORDINATES: protein motif:HMM:NF025159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HOT22457.1"
                     /translation="MTKIHEIIDDIDIENEDLKIAESKVSKEEKNRIFEMALNKANIR
                     KEKTFSRKYILSLAAAMTLILSFAVVFAQGGLSNIYYKLFGENIKYVTEMGTIIDESH
                     SSNGITFNVSNMLGDENSFYIIFEIIKENGESFKDSDYIEFDRFSLDFKSSGGYTWYQ
                     IEDDDKNDNKATFILSGNTKKKISGNKLTISATDITEYSIKEPTDKFDPYNFLIDNQD
                     YIEQNLEKNLQKSTPDIKNNDNSSKEEINKIEEIYQLTPNYVLPWKYVNIPVEEGLNE
                     IYIDNIGFAENKLCIRITLSNKEMHSLGEIYFTNKNNPEDILYNEFMLSEEKNGIKYY
                     YYIFNIENMEELKNYNFIYDIVSKLSTTRGEWNVTFKADYKNTTKTIKVNKEAKINDK
                     RYTVKNIKISPIALNVELKNNFSDNYEDPVHNFSEAISVIMKDGTKIEPTSSGSGTNP
                     LTSYIYLMFEQPINTGQIHKIVIGDIEIEFE"
     gene            complement(1960..2100)
                     /locus_tag="PLD67_07770"
     CDS             complement(1960..2100)
                     /locus_tag="PLD67_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HOT22458.1"
                     /translation="MRRYYLFEPVKEIAEYLNLTPKTVENKLYRGKDKLRTALINKGI
                     II"
     gene            complement(2144..>2325)
                     /locus_tag="PLD67_07775"
     CDS             complement(2144..>2325)
                     /locus_tag="PLD67_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22459.1"
                     /translation="DPKKGLLKNYIISIARFVSINTYNRKISKHILIPLEEDNLEFDM
                     DLDNEVSKSINKRNY"
BASE COUNT          723 a          361 c          321 g          920 t
ORIGIN      
        1 aggattttat tcaatgcaaa attttttccg agtatttaaa aagcttactt atataactcc
       61 aggaaattat aggcagatta atcaaaaatg aaaagttagt ctcaaagata aggtaggtat
      121 ttgttttggt tcctgtcaga taattgggaa cggaaggatg ggaattgatg caatcacaag
      181 gataatcaac catcctaagt tattattaaa atgttaacca agaaaaaaac tgatagaaaa
      241 ggcaaaggta cagtatatga atcaaaacaa agatttgcaa ccgggttaat caaatgtctg
      301 aagatataaa gaacaacata aataatgtaa agcctaatat aaaaactaaa tttttattta
      361 acctaatgca aaaaatgcac acaggtaatg attggaataa gactgaccga gactattgga
      421 ataataataa tttgttagca agtgaaagac cttggaaata aaaagagggc cggcaccgtt
      481 tatgacgccg gtacattttt actcaaattc tatttcaata tccccaatta caatcttatg
      541 aatctgtcct gtatttattg gttgctcaaa cattaggtat atatatgatg ttaagggatt
      601 ggtgccggaa ccgctgcttg tcggctctat ttttgttcca tctttcatta ttactgatat
      661 tgcttcgcta aaattatgca cagggtcctc atagttatcc gagaaattat tttttaattc
      721 tacattgagt gcaataggag aaatttttat attcttaact gtatatcttt tgtcgtttat
      781 ttttgcctct ttatttacct ttatggtttt tgtcgtgttt ttgtaatcag ctttaaaagt
      841 tacattccat tctccccttg tagtgcttaa tttacttaca atatcataaa taaaattata
      901 gttttttaat tcttccatat tttctatgtt aaaaatatag tagtaatatt tgatgccgtt
      961 tttttcttct gaaagcataa attcattata caatatatct tcagggttat ttttatttgt
     1021 aaaatatatt tcacctagac tatgcatttc tttatttgat aatgtaatcc tgatacagag
     1081 cttgttttcc gcaaatccaa tattatcaat gtaaatttca ttcaaacctt cttctacagg
     1141 aatatttaca tatttccaag gcaatacata attcggtgtt aattgataaa tttcttcaat
     1201 tttattaatc tcttcttttg aagaattatc gttgttttta atatctggtg tagatttttg
     1261 tagattcttc tcaaggtttt gctcgatata atcttggtta tctattaaaa agttataagg
     1321 gtcaaattta tctgtcggct cctttatgct gtattccgtt atatcggttg cacttattgt
     1381 caatttattg ccggatattt tcttttttgt attgcccgac aaaatgaatg tagctttatt
     1441 atcgttttta tcatcatctt ctatttgata ccaggtatat ccgcctgagc ttttaaaatc
     1501 caaactgaat ctatcgaact ctatataatc ggaatcctta aagctttctc cattttcttt
     1561 gattatttca aatattatat aaaatgaatt ttcatctccc aacatatttg aaacattaaa
     1621 tgtaataccg tttgatgaat ggctttcatc gattatagtt cccatttcgg tcacatactt
     1681 aatattttca ccaaacagtt tataataaat attggataat ccgccttggg caaaaacaac
     1741 ggcaaatgat aaaatcaaag tcattgctgc agctaatgat aatatatatt ttcggctgaa
     1801 agttttttct tttcttatat ttgctttatt taatgccatt tcaaaaattc tgtttttttc
     1861 ttcttttgat actttgcttt ccgctatttt taaatcctca ttttctatat cgatatcatc
     1921 tattatctca tgaatttttg tcaatttagt caccctttct tatataatta ttcctttatt
     1981 aataagtgcc gttcttagtt tatccttacc tctgtacagc ttattttcaa ctgttttcgg
     2041 ggttaagttt agatactctg ctatttcctt aaccggttca aacaagtagt acctcctgat
     2101 aaatatttcc ttatcggggt ggggaagatt gttgatagta tcattaataa tttcttttat
     2161 taatggattt acttacttca ttatctaaat ccatatcaaa ttccaaatta tcctcttcca
     2221 gaggaattaa aatatgtttt gaaatcttac ggttataagt atttatactt acaaaacgcg
     2281 ctattgaaat tatataattt ttgagcagac ctttttttgg gtcga
//
LOCUS       DAOSOW010000313         2322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_46127_length_2322_cov_3.259374, whole genome shotgun sequence.
ACCESSION   DAOSOW010000313 DAOSOW010000000
VERSION     DAOSOW010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46127_length_2322_cov_3.259374"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..>2322)
                     /gene="leuS"
                     /locus_tag="PLD67_07780"
     CDS             complement(206..>2322)
                     /gene="leuS"
                     /locus_tag="PLD67_07780"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOT22460.1"
                     /translation="VTDDNIKVFTEQLNAAGYSFDWSRSVDTTDPEYYKWTQWIFVQL
                     FKHGLAYKDKTYVNFCNSCKVVLANEDSQNGICDRCGSQVVQMEKDVWFLKIRDYADK
                     LLEGLDEVDYLPRIRIEQENWIGRSYGAEVDFKLKDTEDNLRVFTTRPDTLYGVTFMV
                     MAPEHPLIKKYENIIQNLDEIKEYQEESKKKTEFERVQLAKGKTGVEIKGLTALNPVN
                     GEEVPIWVADYVMMGYGTGAIMGVPGHDQRDWEFAKKYNIPIIEVIGGGNIEEEAFTD
                     VEDGILVNSGMINGLRVKDAIEKITNYLVENNLGERKVNYKMKDWAFNRQRYWGEPIP
                     IIHCPHCGMVPVPEEELPLLLPEVENYRPAETGESPLANIPEWVNVKCPVCDEDAKRE
                     TDTMPQWAGSSWYFLRYIDPHNKEQIASKEKLEYWGPIDWYNGGMEHVTRHMIYSRFW
                     HRFLYDIGVVPFKEPYAKRTAQGLILGADGEKMSKSRGNVVNPNDIIDEYGADTLRTY
                     IMFIGDYEKYAIWNDASVKGCKRFLDRVWKMQEFVNEEKGYSHKHLTLMHKTIKKVSE
                     DFEAMKYNTAIAAMMSFINEIYNDGSITRDELKTFITLLYPVAPHITEEIWQEQGFEG
                     MLNQTEWPVWDEEKTVEDVIELPVQVSGKVRGKILLPKDADVNTARKLAEADENIFKY
                     IEGKTTIKEIYVPGKIYNIVVK"
BASE COUNT          622 a          541 c          329 g          830 t
ORIGIN      
        1 acaacttgcc atcattgcat aataacccat atatcctgtg tatggatatg gatatgtatt
       61 ataatatctt tttatatcaa acataaattc acccctctct ccaatatatg aatttcccct
      121 gttaatagtt ataaaaagat gtttgacaaa tactgacaat aacataaaaa aaatctcccg
      181 accttgtcga gagatttcct aatatttatt ttactactat attgtatatt ttccccggta
      241 catatatttc ttttatggtt gttttgcctt ctatatattt aaaaatattc tcatcagctt
      301 cagccaattt ccttgcagta tttacatccg catccttcgg caataaaatt ttccctctta
      361 cctttccgct tacctggaca ggaagctcta taacatcctc aactgttttt tcttcatccc
      421 atacaggcca ttctgtctga tttaacatgc cttcaaaacc ttgttcctgc catatttctt
      481 cagttatatg gggagccacg ggataaagca gtgtaatgaa tgtttttaat tcatcccttg
      541 taatacttcc atcgttgtaa atttcattaa taaagctcat catggcagct attgcagtat
      601 tatatttcat agcttcgaaa tcttcgctga ccttttttat tgttttatgc ataagagtta
      661 aatgtttatg agaatatccc ttttcttcat ttacaaactc ctgcattttc cagactctgt
      721 ctaaaaatct tttacatcct tttacacttg catcattcca aatagcatac ttttcatagt
      781 ctccgataaa cataatgtat gttcttaaag tatctgcacc gtactcatct attatatcgt
      841 ttggatttac cacatttcct ctcgactttg acattttttc tccgtctgca ccaaggatta
      901 agccttgagc ggtacgttta gcatatggct ccttaaaggg tacaactcct atgtcataga
      961 ggaacctgtg ccagaacctt gaataaatca tgtgtcttgt aacgtgctcc atccctccgt
     1021 tgtaccagtc gatgggaccc cagtattcta acttttcttt tgatgctatt tgctctttat
     1081 tatgggggtc aatatatctt aaaaaatacc atgatgaccc tgcccattga ggcatagtgt
     1141 ctgtttctct ttttgcatct tcatcgcata cgggacattt tacatttacc cattcaggta
     1201 tattggcaag cggtgattct cccgtttcgg caggacgata attttcgact tcaggcagta
     1261 acaagggaag ctcttcttcc ggcacgggaa ccataccgca atgaggacaa tgaataatag
     1321 gaatcggctc accccagtat ctctgcctgt taaaggccca atccttcatc ttgtaattta
     1381 cttttctttc tcctaaatta ttttcaacca aataatttgt aattttttca attgcatcct
     1441 taactctcaa accgttaatc ataccggaat ttaccaatat tccgtcttct acatccgtaa
     1501 atgcttcttc ttcgatattt cctccgccga ttacttcaat tatgggaata ttgtattttt
     1561 ttgcaaactc ccagtctctt tggtcatgac cgggaacacc cataatagca cctgttccat
     1621 accccatcat aacatagtcg gcaacccaaa taggcacttc ttccccattg acgggattaa
     1681 gagcagtcag tccttttatt tctactcctg tctttccttt tgctaactgc accctttcaa
     1741 attcggtttt cttttttgac tcttcctggt attcttttat ttcatccaga ttttggatta
     1801 tattttcata ctttttaatc aaaggatgtt ccggagccat taccataaat gtaacaccgt
     1861 acaaggtatc cggtcttgta gtaaatactc ttaaattgtc ctctgtgtcc tttaatttaa
     1921 aatcaacttc ggcgccataa gaacgaccta tccagttttc ctgctcaatt cttatacgcg
     1981 gcaaataatc aacttcatcc agaccctcta aaagcttatc cgcataatct ctgattttta
     2041 gaaaccatac atctttttcc atctgcacta cctggctgcc gcatctgtcg catatgccgt
     2101 tttgcgaatc ttcatttgca agtacaacct tacagctgtt gcagaagttt acatatgttt
     2161 tatccttata agccagccca tgcttaaata attgaacaaa tatccattga gtccatttat
     2221 aatactccgg gtctgtcgta tcaacacttc ttgaccagtc gaaggaatag ccggcagcat
     2281 tgagctgttc ggtaaaaacc ttgatgttat catcagtaac ct
//
LOCUS       DAOSOW010000314         2308 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_46510_length_2308_cov_3.126498, whole genome shotgun sequence.
ACCESSION   DAOSOW010000314 DAOSOW010000000
VERSION     DAOSOW010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46510_length_2308_cov_3.126498"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PLD67_07785"
     CDS             complement(<1..543)
                     /locus_tag="PLD67_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22461.1"
                     /translation="MKKITIIFIALLIISSMNICYAANRNLRGKFTEKDVPGTNFKEI
                     TLTPYYAGADEGDEEEDTYYIYLPTGYFVEGKSASYVADRNGSYPFTVYDGSTKKTFV
                     YKVDNIEAPEEDDRKSVYIDFRLMYDYEKNQIFFNLKTDKQRTITTPQGTTVTNEINY
                     FIGKIANNVPYDLLITIDETP"
     gene            complement(540..1181)
                     /locus_tag="PLD67_07790"
     CDS             complement(540..1181)
                     /locus_tag="PLD67_07790"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOT22462.1"
                     /translation="MIDTDKIVKIYDEDSKLIGKGQLLSLNEKIIKVKGTGLPILSRK
                     TNVIIEIYSEFVGISRYCCRIDLASDNQLNAHIVKKKPDIERRNSLKVRTDLSYYVES
                     LYRNDKDITKDFPNMKINLINLSIGGMLISSNYELYINDIITFNFQYKKTAVLLKAKV
                     IRIDKIYDENQKNLFFYNYGCIFEEMPPNIEMIITRYLYIRQLEMHKDKWEKL"
     gene            complement(1204..1485)
                     /locus_tag="PLD67_07795"
     CDS             complement(1204..1485)
                     /locus_tag="PLD67_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOT22463.1"
                     /translation="MSQYNNKSNHAVALKYNAAKNNAPVVIASGSGYIADKVVEIAEK
                     NGVPVYRDDSLSAILSQLKIGSEIPEELFSSIVDIYVYFLNFKMPESEA"
     gene            complement(1472..>2308)
                     /locus_tag="PLD67_07800"
     CDS             complement(1472..>2308)
                     /locus_tag="PLD67_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22464.1"
                     /translation="FNELTGKFIYDNTNAMDSNLELLKNEILPFIGRYISKMNDFGII
                     RDYVSVLVHNLVRLEYGSKTNFSDELENLFEFLKYNFQLDEKQMELLKTSLINTYEIK
                     SSIKNDSIESFFKLLDKGISESENAVTKGLMGDMTESLLFSQNVNIPLLHMFLPLNYK
                     GMFMFSEIWIGKDEDSPDNKRNLENNKSYKVFITFDIQNLGYFETTLKLRDSVLSLDI
                     YIPGSLSEQTEKIKSDLNFLLSKNNVTIESIRVQECIKARRFSEVFTNLTERKNGVDV
                     TI"
BASE COUNT          700 a          407 c          329 g          872 t
ORIGIN      
        1 aggcgtttca tcaattgtga ttaacaggtc atagggaaca ttgtttgcta ttttaccgat
       61 gaaataattt atttcgtttg tgacggttgt accttgaggt gttgttattg tcctttgctt
      121 atcagtcttc aaattaaaga atatttggtt tttttcataa tcatacatga gcctgaaatc
      181 tatataaaca ctttttctgt catcttcctc aggtgcttcg atattatcta ctttataaac
      241 aaatgttttt ttggtacttc cgtcatagac tgtaaaggga taacttccat tcctgtctgc
      301 cacataggaa gcactttttc cttcaacaaa ataaccggtg ggtaaataaa tgtagtaagt
      361 atcttcttcc tcatcacctt cgtctgcacc ggcataataa ggcgttaagg tgatttcctt
      421 aaagttggtt ccgggtacat ctttttcagt aaattttcct cttaaatttc tgtttgcagc
      481 ataacatata ttcatactgc ttatgattaa aagagcgata aatataattg taatcttttt
      541 cataatttct cccacttgtc tttatgcatt tcaagctgcc ttatatacaa ataccgagta
      601 ataatcattt cgatattagg aggcatctct tcaaaaatac atccgtaatt gtagaaaaat
      661 aaattctttt gattttcgtc ataaatttta tcaattctaa taactttagc ctttaacaat
      721 acagctgttt ttttatattg gaaattaaaa gtaatgatat catttatata taattcgtag
      781 tttgatgaaa tcaacatgcc gccaatgctt aagttaatca aattaatttt catatttgga
      841 aaatcttttg ttatgtcttt atcatttctg taaaggcttt ctacataata ggataagtcg
      901 gtccgaacct ttaatgagtt tcttctttca atatcgggct tctttttaac tatatgagca
      961 ttaagctgat tatcggatgc caaatcaatc cggcagcagt aacgggaaat tcctacaaat
     1021 tcactgtaaa tttcaataat aacatttgtt ttccggctta aaatcggcaa gccggtacct
     1081 ttaaccttta ttattttttc atttaagctt aacagttgac cttttccaat cagtttacta
     1141 tcttcatcat atatttttac tattttatca gtatcaatca tgattactcc caagctgtta
     1201 tttttaagcc tcactctcag gcattttaaa attcaagaaa taaacataaa tatctacaat
     1261 tgagctgaat aattcttcgg gaatttcgct gcctatttta agctgggaca atatggctga
     1321 caaggagtca tctctataga caggcactcc gtttttttcg gctatttcaa caactttgtc
     1381 tgcaatataa cctgaacctg aagcgattac gacaggagcg ttgtttttag ccgcattata
     1441 tttaagggca acagcatgat ttgatttatt attatattgt gacatctaca ccatttttcc
     1501 tttcagtcag atttgtgaaa acttcgctaa accgcctggc tttaatgcat tcctgcactc
     1561 ttatgctttc tattgtaaca ttgtttttgc ttaagagaaa gtttaaatca cttttaattt
     1621 tctcggtctg ttccgataaa ctgcccggta tataaatatc aagcgacaag acggagtccc
     1681 ttaattttaa agttgtttca aaatatccta agttttggat atcaaaagtt ataaaaactt
     1741 tataggattt gttattttcc aagtttcttt tattatcagg gctgtcttca tccttgccta
     1801 tccatatttc ggaaaacata aacataccct tgtaatttaa cggtaaaaac atgtggagta
     1861 agggaatatt aacattttga ctaaataaaa gcgactcggt catatccccc attaaacctt
     1921 tagttaccgc attttcactt tcgcttatcc ctttatcaag gagtttgaaa aatgattcaa
     1981 tagaatcgtt cttaatgctt gattttattt cataagtatt gattaaagat gtttttaata
     2041 attccatctg tttttcgtca agttgaaaat tatattttaa gaattcaaac aaattctcca
     2101 attcatcaga aaaattagtt tttgagccat attccaatct tacaaggttg tgaaccaata
     2161 cggatacata gtcccttata ataccaaaat cattcatttt ggaaatatat cttcctatga
     2221 agggcagaat ttcatttttt aacagctcca ggtttgagtc catggcattt gtattatcgt
     2281 aaataaattt tcccgtaagc tcattaaa
//
LOCUS       DAOSOW010000315         2298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_46805_length_2298_cov_3.736514, whole genome shotgun sequence.
ACCESSION   DAOSOW010000315 DAOSOW010000000
VERSION     DAOSOW010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46805_length_2298_cov_3.736514"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="PLD67_07805"
     CDS             complement(<1..1025)
                     /locus_tag="PLD67_07805"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOT22465.1"
                     /translation="MNKKTLKDINVKNKKILVRCDFNVPLDKEGNITDDIRIKASLPT
                     INYLLDNGAAVILLSHLGRPDGEANKKYSLQPVAQRLSSLLGRGVCFEDCDVVVDDRV
                     KTNAAKLKPGDIMLLQNTRFRKEETKNAGTFAKELASLGELFVNDAFGTAHRAHSSNV
                     GICEYLHSALGFLVEKEVEIMGNALKTPKRPLTAILGGAKVSDKIAVIENLLNIADNV
                     LIGGGMMYTFLKAKGYNTGNSLLEEDKVELAKNIIEKASEKVVKLILPVDTVVAKEFK
                     NDTEFFTTDVDKIPDEYIGLDIGEKTVEIYKEIIGNSKTIIWNGPLGVFEMGNFANGT
                     NAVAKAIA"
     gene            complement(1100..2107)
                     /gene="gap"
                     /locus_tag="PLD67_07810"
     CDS             complement(1100..2107)
                     /gene="gap"
                     /locus_tag="PLD67_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOT22466.1"
                     /translation="MVRVAINGFGRIGRLALRLMAQDEDFKVVAINTNSDAETLTYLL
                     KYDTAHGNFMTDKISYKNNLMYVDGKEIITSRVKEPENCNWKELDIDLVIDCTGKFND
                     KEKAEAHIKAGAKKVLLSAPGKGDLKTVVYNVNHNILDGSETVVSAASCTTNCLAPLA
                     KVLNDEFGIVKGLMTTVHAYTNDQNIHDNSHKKGIMARRGRAAAANIVPASTGAAKTV
                     GVVLPELKGRLDGIALRVPTLTGSVVDLTVELGKNVTVEEVNQAVKNAANETIAYTED
                     PIVSSDVIGSRYACVFDALSTQIIEADGKQLVKLIAWYDNEMSFTAQLVRVAKYWGQM
                     R"
BASE COUNT          648 a          476 c          326 g          848 t
ORIGIN      
        1 gcaattgctt ttgctactgc attggtcccg ttggcaaagt tgcccatttc aaaaacacct
       61 aagggaccgt tccaaattat tgtttttgaa tttcctatta tttctttata tatctcaaca
      121 gtcttttcac cgatatcaag tcctatgtat tcatcaggaa ttttatcaac atctgttgtg
      181 aagaattccg tatcattttt aaattctttt gctactactg tatcaaccgg taatattaat
      241 tttactactt tttctgatgc tttctcaatt atattcttgg caagttcaac tttatcttct
      301 tccaaaagag agtttcccgt attatatcct tttgccttaa ggaaagtata catcatacct
      361 ccgccaatta aaacattatc agcaatattc aataaatttt caattactgc tattttatcc
      421 gaaacctttg ctccgcctaa gatggcagta agaggtcttt ttggagtttt taatgcattt
      481 cccattatct caacttcttt ttctaccaaa aacccaagtg cagaatgaag atattcacag
      541 atgcccacat ttgaggaatg tgctctgtga gcagtgccga atgcatcatt aacgaacagt
      601 tctcccagtg atgccagctc ttttgcaaat gtacctgcat tcttcgtttc ttcttttcta
      661 aacctagtat tctgcaaaag cattatgtct ccaggtttaa gctttgctgc atttgttttt
      721 actctatcat ctactacgac atcacagtct tcaaaacaaa cacctcttcc taagagtgag
      781 cttaatcttt gtgcaacagg ctgcagagaa tatttcttat ttgcttctcc atcaggtcta
      841 cccaaatggg acaataatat tactgccgca ccattatcca aaaggtaatt tattgttgga
      901 agagatgctt taattcttat atcatcagtt atgttcccct ctttgtcaag gggaacatta
      961 aaatcacatc ttactaatat ttttttattt ttaacgttta tatcctttaa agttttttta
     1021 ttcatgataa acctccgtta acgtttatat gtcattaagg gttgaccata tctctacgcg
     1081 gtcgaagtct ttggttaaat catctcattt gtccccaata cttagctact ctcacaagtt
     1141 gcgctgtaaa tgacatttca ttgtcatacc aggcaattaa tttaaccagc tgtttaccgt
     1201 cagcttctat tatttgagtg gacaatgcat caaatacaca tgcatatctg cttccaataa
     1261 catcgcttga tacaatcggg tcttcagtat atgctattgt ttcattggct gcatttttaa
     1321 cagcttgatt tacttcttca acagtaacat tttttccaag ttcaacggtt aaatctacaa
     1381 ctgaaccggt aagagtggga acacgaagag ctataccgtc aagcctgccc tttaattcag
     1441 gcaaaactac tccaacggtc ttagcagctc ctgtagatgc aggaacgata tttgctgctg
     1501 ctgcacgtcc tctccttgcc attatacctt ttttatgcga gttatcatga atattttggt
     1561 catttgtata agcgtgaaca gtggtcataa gtcctttaac tattccaaat tcatcattta
     1621 atacttttgc aagcggagct aaacagtttg ttgtgcatga tgctgcagat acaaccgttt
     1681 cacttccatc taatatattg tgatttacat tgtatacgac tgtttttaaa tcgcctttgc
     1741 ccggcgctga aagcaatacc ttttttgctc ctgccttaat atgagcttct gctttttcct
     1801 tgtcgttgaa ttttccggtg cagtcaatta ccaaatcaat gtctaattct ttccaattgc
     1861 agttttcggg ctcttttact cttgatgtta ttatttcttt gccatcaaca tacattaaat
     1921 tatttttgta ggaaatttta tcggtcataa aattaccgtg cgcagtatca tactttaata
     1981 aataagtcaa tgtttcagca tctgaatttg tgttgattgc tacaaccttg aaatcttcat
     2041 cctgagccat aagccttaat gctaatcttc caatcctgcc aaatccgtta attgctactc
     2101 ttaccattta atcctcctat agaaataaca tttattataa ttctaaatcc ttattaataa
     2161 atccgttgca ataaggataa tatgtaacaa acagattata tcatactaaa tatataatgt
     2221 gtagcatatt ataataaaaa aatatgcata tctttttgca tatttttcta ttaatatctt
     2281 ctcctcatgg aagatgac
//
LOCUS       DAOSOW010000316         2296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_46861_length_2296_cov_3.718876, whole genome shotgun sequence.
ACCESSION   DAOSOW010000316 DAOSOW010000000
VERSION     DAOSOW010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46861_length_2296_cov_3.718876"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="PLD67_07815"
     CDS             <1..630
                     /locus_tag="PLD67_07815"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOT22467.1"
                     /translation="LHVDAVQSYGKIKIDVNKTPLDTLSFSSHKIHGPKGVGGLFIRS
                     GIKITPLIYGGGQERGIRSGTENTTGIIGFGKACKLMYDNFDEEVNKLYGLKNTYIKR
                     LEEIEDIKINSPIDGAPHIISVSFKNVPGEVLVHYLEDKNIFVSTGSACSSKSKDSRI
                     LEAIKLSKEYRAGTIRISLGHFNNFEEVDYVIDNIKKSVKDIRSITKRG"
     gene            632..1792
                     /gene="thiI"
                     /locus_tag="PLD67_07820"
     CDS             632..1792
                     /gene="thiI"
                     /locus_tag="PLD67_07820"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002838207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HOT22468.1"
                     /translation="MYNIAAVSFGELFLKGKNRGKFEQKLIEQIKYALKEFNHVSVYK
                     DSGKVYVETKNEEDMDKIVEKVRKIFGISNISPSVKTSKDVDIIIEKTIELFRYLLSK
                     EDIKTFKIKTKRSDKEFPLKSMDFSALVGGKILENFSFVKVDVHTPDVEVYIDIKKCC
                     YISSERIKTLGGLPLGSNGRALLLLSGGIDSPVAGYMIAKRGVEIDALYFHTYPFTSE
                     RANEKVIKLKQKLEEYCGKIKLYSINMLEIHKAIREFCKEEETTILARRFMMRIAEKI
                     ALENKIDMLITGESLGQVASQTMKSMAVIEDAINMPILKPLVGLDKSEIIEIAREIGT
                     YETSILPYDDCCSVFAPQHPLINPKLESIKRSESNLNVEELIEKVLSTLEIL"
     gene            1876..2016
                     /locus_tag="PLD67_07825"
     CDS             1876..2016
                     /locus_tag="PLD67_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22469.1"
                     /translation="MSDEVRATNFRCCDDRSNDWFEWLIILGIIFFLLGGNNFFGRGF
                     GR"
BASE COUNT          854 a          308 c          459 g          675 t
ORIGIN      
        1 ctgcatgttg atgctgttca atcctatggg aaaattaaaa tagatgtaaa taaaactcct
       61 cttgatacct tgtcgttcag cagccacaaa atacacgggc ctaagggagt aggcggatta
      121 tttatacgct ccggtataaa aataactcca ttaatttacg gaggaggaca ggagagaggg
      181 attcgctcgg gaaccgagaa cacaacagga attataggct tcggcaaggc ttgtaaatta
      241 atgtatgata attttgatga ggaagtaaat aagctttacg gtttaaaaaa tacatatata
      301 aaaaggcttg aagaaattga agatattaaa ataaacagtc ctatagacgg agctcctcat
      361 attattagtg tatcatttaa aaatgtgccg ggagaagtat tggttcatta tttggaagat
      421 aaaaatattt ttgtttcaac cggctctgct tgctcttcaa aatcaaaaga cagcaggata
      481 ttggaagcaa taaaactaag caaggaatac cgggcaggaa ctatacgcat cagtcttggt
      541 cattttaata actttgaaga agtcgattat gtaattgata atattaaaaa atcggttaaa
      601 gatataagaa gtataacaaa gagaggataa tatgtacaat atagctgcag taagcttcgg
      661 agaattattt ttaaaaggaa aaaacagagg aaaatttgag caaaagctga ttgagcaaat
      721 caaatatgct ctgaaagaat ttaatcatgt gtccgtatac aaagattcag gcaaggtata
      781 tgttgaaacg aaaaatgaag aggatatgga taaaatagtc gaaaaggtaa ggaaaatttt
      841 tggcatatcc aatataagtc cttccgttaa aacaagcaag gatgtagata taattattga
      901 aaaaacaatt gaactgttta gatatctgtt aagtaaggag gatataaaaa catttaaaat
      961 taaaaccaag agaagcgaca aggaatttcc ccttaaatca atggatttca gtgccttagt
     1021 gggcggtaaa atcttggaga atttcagctt tgtaaaagtt gatgtccata cccccgatgt
     1081 ggaagtatat attgatatta aaaaatgctg ctatatatct tccgaaagaa taaaaacatt
     1141 gggcggtctt ccccttggct cgaacggaag agcactgcta ttactttcag gtggaattga
     1201 cagccctgtt gcaggatata tgattgcaaa aagaggtgtt gaaatagatg cactttattt
     1261 tcatacatat ccttttacgt ctgagagagc aaatgaaaaa gtaattaagc tgaaacaaaa
     1321 acttgaagaa tattgcggga aaataaaatt atacagtatt aacatgcttg aaattcacaa
     1381 agcaataaga gaattttgca aagaagaaga aactaccata ttggcaaggc gttttatgat
     1441 gcgtattgca gaaaaaattg cattggaaaa taaaatcgat atgcttatta cgggagaaag
     1501 cctgggtcag gttgcaagcc agacaatgaa gtcaatggct gttattgaag atgcaatcaa
     1561 tatgcctatt cttaaacccc tggtgggatt agataaatct gaaataatag aaattgctcg
     1621 agaaataggc acctatgaga cttcaatttt gccatatgat gactgctgct ctgtatttgc
     1681 tccacagcat cctttaataa atcctaagct tgagagtatt aaaaggtctg aaagcaattt
     1741 aaatgtcgaa gaattaatcg aaaaagtatt atcaacttta gaaattttat aataaaaaca
     1801 agaaaacatt tcagtgttat cacatatttt tttatctgac atatattgaa ttataaatta
     1861 agagaggtga tttatatgtc agatgaagta agagcaacta atttcagatg ctgcgatgac
     1921 agatctaatg attggttcga atggttaatt atattgggga ttatattttt cttgttagga
     1981 ggaaacaatt tcttcggaag aggattcggc agatagtact tactgaaatg cggctttaga
     2041 ggccgtattt tttttatctc ttgattttct attatatagg ttgtatacta atctttaata
     2101 tagaggagga tgttgacaaa atgtttaaag tagcactttt ttgtgacata ccccaggaaa
     2161 ctttaaattt gcttcctgag gataaatatt atttaataag agaagaaaca tcagaagctg
     2221 acggaattat aataagaagt ttggatattc atgatatgaa gttttcaaaa cagctcaaag
     2281 ccatagccag gtcggg
//
LOCUS       DAOSOW010000317         2286 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_47133_length_2286_cov_3.287763, whole genome shotgun sequence.
ACCESSION   DAOSOW010000317 DAOSOW010000000
VERSION     DAOSOW010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47133_length_2286_cov_3.287763"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1759
                     /gene="iorA"
                     /locus_tag="PLD67_07830"
     CDS             <1..1759
                     /gene="iorA"
                     /locus_tag="PLD67_07830"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HOT22470.1"
                     /translation="GNEAVAQGAYEAGIHFAAAYPGTPSTEILENIALRKEITAEWAP
                     NEKVAVESAFGASLAGARTLTAMKHVGLNVAADPFFTIGYTGVNGGMVLVSADDPGLH
                     SSQNEQDNRYYARMAKVAMLEPSNSQECKDMVIRAMEISEKYDVLVMLRMTTRVCHSK
                     SIVETGERIEVPVKPYVKNAPKYIMAPANAKAAHVVVEKKLKELEKFSNETELNYIEW
                     NDKKIGVISAGVAYQYAKEVFGKDASYLKLGFTFPLPMEKIKKFAEEVETLYVVEELE
                     PFMEEQIKAAGIKCIGKEKIPNTWELNPDIVKKSIFGTENEVIDYDRTLAVGRPPTLC
                     AGCPHRAFYYQLSKKKNTIVTGDIGCYTLGGAPPLNSMDTCVCMGASISMAHGAKKAF
                     ERNNDDKRVVAVIGDSTFFHSGMTSLLDVVFNKGNTITCILDNRITAMTGHQDNPGTG
                     YTLQGDSTDVVQIEPIVRALGVKNIKTVNPMNQAEMKAALNWAYDLNEPSVIISRYPC
                     ALKKYTKEDLEEFGPIQGYCEVDEETCIGCKICVGTGCPAIVFKKDIKKSSINKFQCV
                     GCELCLQLCPTKAISKVGE"
     gene            1761..>2286
                     /locus_tag="PLD67_07835"
     CDS             1761..>2286
                     /locus_tag="PLD67_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOT22471.1"
                     /translation="MKDTKNILLVGVGGQGTILVSKILSTGLMTAGYDVKMSEIHGMA
                     QRGGSVSTQVRYGSEVFSPIIGIGEADILVSFETMETLRWLEYLKPDGVVVINDFEIA
                     SAPILMGKAEYPEGIIELIKGKAKTKVINAGETAEKLGNQKVMNVVLLGALIKSMNLT
                     DIDWEAVIKSQVKEK"
BASE COUNT          821 a          346 c          531 g          588 t
ORIGIN      
        1 aggaaatgaa gcagttgctc agggagctta tgaagcagga attcattttg ctgcagcata
       61 tcccggaacg cctagtacag aaatattgga aaacatagcc cttcgcaagg agataacagc
      121 agaatgggca ccaaatgaaa aagtggcagt tgagtctgca tttggagcaa gccttgcagg
      181 agcaagaact ttaacagcta tgaaacatgt tgggcttaat gttgcagccg acccgttttt
      241 cacaatcggt tacacaggtg taaacggcgg aatggtactg gtaagcgcag acgacccggg
      301 gcttcattct tctcaaaatg agcaggataa caggtattac gcaagaatgg caaaggtagc
      361 tatgcttgag ccaagcaact cacaggaatg taaggacatg gtaataagag ccatggaaat
      421 aagcgaaaag tatgatgtct tggtaatgct gagaatgact acaagagttt gccacagtaa
      481 atcaattgtt gaaacaggcg aaagaataga agttcctgta aaaccatacg tgaaaaatgc
      541 gccaaaatat attatggcac ctgctaatgc aaaagcggca catgtggtag ttgagaaaaa
      601 gcttaaagaa cttgaaaagt tttcgaatga aactgagttg aattatatag aatggaatga
      661 caaaaaaatc ggtgttattt cggccggagt tgcttatcaa tatgcaaaag aagtattcgg
      721 aaaggatgca tcatatttaa aattaggatt tactttcccg cttccaatgg aaaaaattaa
      781 aaaatttgca gaagaggttg aaactcttta tgtagttgaa gaattagaac cttttatgga
      841 agaacagata aaagctgccg gtattaaatg tataggcaaa gaaaaaatcc ccaatacatg
      901 ggagcttaat ccggatattg taaaaaaatc aattttcggt actgaaaatg aagtaattga
      961 ttatgacaga acattagcag tgggaagacc tcctacacta tgtgccggtt gtcctcaccg
     1021 tgcattctat tatcaattgt caaagaaaaa gaacactata gtaacaggtg atataggatg
     1081 ttatacattg ggcggagcac ctccgttaaa ttcaatggat acatgtgtat gtatgggggc
     1141 aagcataagc atggcacatg gtgctaaaaa ggcatttgaa aggaacaatg atgataaaag
     1201 agtagttgca gttataggag attcaacatt cttccattca ggtatgacaa gcttgctgga
     1261 cgtggtgttt aacaaaggaa ataccataac ttgtatttta gacaatagaa taacggcgat
     1321 gacaggacat caggataatc cgggaacagg ctatacactt cagggagata gtactgatgt
     1381 ggttcaaatt gagcctatag tcagagcatt aggtgttaaa aacataaaaa cagtaaatcc
     1441 gatgaatcaa gctgaaatga aagctgctct taattgggct tacgatttaa acgaaccatc
     1501 ggtaataatt tcaagatacc catgtgctct taagaaatat acaaaagaag acctggaaga
     1561 attcggacct attcaaggtt attgcgaagt tgacgaagaa acatgtatag gatgtaaaat
     1621 ttgcgtaggt acaggttgtc cggcaatagt gtttaaaaaa gacattaaga aatcttcaat
     1681 aaataaattt caatgtgtcg gttgtgagct ttgtctacag ttatgcccca ctaaggcaat
     1741 cagtaaggta ggtgaataaa atgaaggata ctaaaaatat tttattggta ggtgttggcg
     1801 gtcagggaac aatattggta agtaaaatat tatcaacagg tcttatgaca gccggttatg
     1861 acgttaaaat gtcagaaatt cacggaatgg cacaaagagg aggcagtgtt tcgactcagg
     1921 taagatatgg aagtgaagta ttttcgccta ttatcggaat cggtgaagcc gatattctgg
     1981 tttcctttga aacgatggaa acattgagat ggcttgaata cttaaagcct gacggagttg
     2041 ttgtaatcaa tgatttcgaa attgcttccg cacccatatt gatgggaaaa gccgaatatc
     2101 ccgagggaat aatcgaattg attaaaggga aagctaaaac aaaggttata aatgccggag
     2161 aaacagctga aaaactaggt aatcagaaag ttatgaacgt ggttttatta ggggccttga
     2221 ttaagtcgat gaacttaacc gatattgatt gggaagctgt aataaaaagc caggtaaaag
     2281 aaaaat
//
LOCUS       DAOSOW010000318         2277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_47377_length_2277_cov_2.651665, whole genome shotgun sequence.
ACCESSION   DAOSOW010000318 DAOSOW010000000
VERSION     DAOSOW010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47377_length_2277_cov_2.651665"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="PLD67_07840"
     CDS             complement(<1..835)
                     /locus_tag="PLD67_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22472.1"
                     /translation="MQQDKVIVMPFLRNRNIENAASKLNVAYKDAEENFPEDNHNDVY
                     LEISNKLSNLISNIHGESQLIELFAQGGKIPDKKYLTNGVNAIRQNCLKITKTINNLI
                     ELRKLEKKQTCLVVNSVNIVEIIDNIVINASKHIKQEIIFDTNIEEKYMSCDINKLQK
                     AILIILSVAVRYSNKKEILVNLNVFEDNINITVSFNNNSKLLNFFIQKMDNLTPDNLD
                     ELSLSFYLCKSLIALHEGSISVEGNSYETMFTIKLPCENTDSINYLFMRDNKNEYLAE
                     QI"
     gene            1181..1465
                     /locus_tag="PLD67_07845"
     CDS             1181..1465
                     /locus_tag="PLD67_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22473.1"
                     /translation="MAFLNKIGKAIGEAAGTASEKAKDFAETTKLKNAISAEEKQINQ
                     YFLEIGKYVFEQERNNPDSPLAEVFSKITDSLRNIEELKRKITEIKLDNN"
     gene            complement(1532..1906)
                     /locus_tag="PLD67_07850"
     CDS             complement(1532..1906)
                     /locus_tag="PLD67_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOT22474.1"
                     /translation="MFFIAGIYPKREELDYYEPIMCSCCTKYGRFEAYVEYSVLSLFF
                     IPLFKFNKKYYARTTCCNSLYLITNKEKGLMMERGQGHNVFLKDTDITLIHKGICENT
                     CPNCGFKVSEEHKYCPTCGNKL"
     gene            complement(1916..>2277)
                     /locus_tag="PLD67_07855"
     CDS             complement(1916..>2277)
                     /locus_tag="PLD67_07855"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT22475.1"
                     /translation="NDKYGHLAGDSVAIKIAETISKSIRDTDIFARWGGDEFVLLLPN
                     TDVQEAENMAERMRECISNNLCDPISNITCSFGVAAYEKNDTTQSLLNKADKLLLQAK
                     ASGKDRVVSMDSCTAQN"
BASE COUNT          759 a          390 c          302 g          826 t
ORIGIN      
        1 ctatttgctc agccaaatat tcatttttat tgtctcgcat gaacaaataa ttaattgaat
       61 ctgtattttc gcatggaagc tttatagtaa acattgtttc ataagaattt ccttcgacag
      121 aaatacttcc ttcatgcagg gcaattaatg atttacataa ataaaagctc aaagataatt
      181 catctaaatt atccggcgta aggttgtcca ttttttgaat aaagaagttc aataacttac
      241 tattattatt aaatgaaaca gtaatattaa tattatcttc aaaaacattc agattaacta
      301 atatttcctt cttgttggag tacctgacgg ctacggataa aataattaga attgcttttt
      361 gaagcttatt gatatcacat gacatatatt tttcttcaat attagtatca aaaattattt
      421 cttgtttgat atgctttgat gcgttaataa ctatattatc aataatttcg acaatattta
      481 cagagtttac cactaaacaa gtttgtttct tttcaagttt tctcaattcg attaaattgt
      541 ttatagtctt tgttatcttt aaacaatttt gtcgtattgc attaacccca tttgtcaaat
      601 attttttgtc cggaattttt ccaccttgtg caaaaagctc aataagctga ctttctccat
      661 gaatattact tataagattg cttaatttat ttgaaatttc caaatataca tcattatgat
      721 tatcttcagg aaaattttcc tctgcatcct tatatgctac attcaatttg cttgctgcat
      781 tttcgatatt tctgtttctt aaaaaaggca ttacaattac tttgtcttgt tgcaatatta
      841 tcacctcccc ccgctattaa ttaccataca tattatttag atttttatca taaaaaatat
      901 aataaaatca aaaaaggtca caaacgtaat cattataaat tgctaaatcg taaaaatgtg
      961 tcgaaatttg gcgctaataa aaaatatttt tgacgcgact taatccttgc tttgtcgtat
     1021 tttgcaacaa cactacaagt tatctgttaa atcaccctca gggtgattgc aaataattca
     1081 cattagtata aaataagtat ataaagtttt catcataaat gtttaaaaaa agaaatataa
     1141 ggtatataaa taacaatata tttgattgga ggggttaacc atggcattct taaacaaaat
     1201 aggcaaggca ataggtgaag ctgccggaac ggcttctgaa aaggcaaaag attttgctga
     1261 aacaaccaaa ctcaaaaatg caatttcagc tgaggaaaaa caaatcaacc aatatttttt
     1321 ggaaatcgga aaatatgttt ttgaacagga aagaaataat ccggatagtc ctttagcaga
     1381 agttttcagc aaaattaccg attccttaag aaatattgag gaattaaaga gaaaaattac
     1441 tgaaataaag cttgataaca attgattgaa aggagcggta ttgatacaat actgctccta
     1501 tattaaaatc aatagatgtt gttttccctt tttataattt attcccgcag gttgggcaat
     1561 atttatgctc ttcgcttact ttaaagccgc agttggggca tgtattttca catattcctt
     1621 tatggattag cgttatgtct gtatctttca aaaaaacatt gtgtccttgt cctctttcca
     1681 tcattagacc tttttctttg tttgttatta aatacaagct attgcagcaa gtcgttcttg
     1741 cataatattt tttattaaat ttaaacaatg gtataaaaaa taaacttaat acactatatt
     1801 ccacatatgc ttcaaatcga ccgtattttg tgcagcagct gcacattatc ggttcataat
     1861 aatctaattc ttctcttttt ggatatatac ctgcaataaa aaacataaag acctcctaat
     1921 tctgagcagt acagctatcc atgctcacga ctctatcctt cccgcttgcc ttagcttgta
     1981 aaagtaattt atctgcttta ttaagcaatg attgagtcgt atcatttttt tcataagctg
     2041 ctacaccaaa actgcaagta atattactta tcgggtcgca caagttatta ctgatacatt
     2101 ctctcattcg ttcagccata ttttctgctt cctgaacatc tgtatttggc agaagaagca
     2161 caaattcatc cccgccccat ctggcaaaaa tatctgtatc cctaatcgat ttactaattg
     2221 tttcagcaat ttttattgct acgctgtcac ctgccaaatg cccatattta tcattta
//
LOCUS       DAOSOW010000319         2272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_47522_length_2272_cov_3.726207, whole genome shotgun sequence.
ACCESSION   DAOSOW010000319 DAOSOW010000000
VERSION     DAOSOW010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47522_length_2272_cov_3.726207"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /gene="purB"
                     /locus_tag="PLD67_07860"
     CDS             complement(<1..1340)
                     /gene="purB"
                     /locus_tag="PLD67_07860"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOT22476.1"
                     /translation="MSVKYENPLCKRYASSEMQFIFSDDNKFSTWRRLWIALAESQKE
                     LGLDITQNQIDEMISNVKNINYEAANKYEAEVRHDVMAHILAYGEQCPSAKPIIHLGA
                     TSCYVGDNTDIILMRSAMLLIKRLLVNAIDKLWTFADKYKSLPCLAYTHFQAAQPTTV
                     GKRATLWLADLLLDLEQLDFTLCNLKLLGCKGTTGTSASFLSLFDGDHKKATQLEEKI
                     AIKMGFDAVYSVSGQTYSRKVDYYVLSVLSGIAQSAYKFSNDIRLLSHLKEFDEPFED
                     KQIGSSAMAYKRNPMRSERIASLSRYVIVNTLNPALTASSQWFERTLDDSANKRISIP
                     EAFLAADAILSLYINVISGLTVYPKIIEKHLAEELPFMVTENILMYCVKKGGDRQELH
                     ERIRKHSVEAAKEIKQNGSDNDLIKRILNDKTFNISEQEMKDILNAENFCGRAK"
     gene            complement(1330..2034)
                     /locus_tag="PLD67_07865"
     CDS             complement(1330..2034)
                     /locus_tag="PLD67_07865"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOT22477.1"
                     /translation="MKDILYEGKSKIVYSGEDNESLLIKFKDTATAFNGKKKEELMEK
                     GQLNAEISNIIFNYLSKNGIKTHLLKVIDERSVLVKKAEIVMVEVIVRNIAAGSFSNK
                     YGVPEGTVLNNTIIEFSLKSDELGDPMINESQITAIGIASKDEIYEMKESALKINALL
                     SDLFDKAGITLVDFKLEFGRTREGIILCDEISPDSCRLWDKETGKKLDKDRFRRDLGD
                     VLGGYREVLRRLKNVC"
BASE COUNT          638 a          464 c          373 g          797 t
ORIGIN      
        1 tctttagctc ttccgcagaa attttcggca tttaatatgt ctttcatttc ttgttcagat
       61 atattaaacg ttttgtcgtt taaaatacgt ttgattaaat cattatcgct gccgttttgt
      121 tttatctctt ttgcagcttc aacagagtgc tttcgtattc tttcgtgaag ctcctgcctg
      181 tcaccgccct ttttaacaca atacatcaag atattttcgg taaccataaa aggcagttct
      241 tcagccaggt gcttttctat tattttcgga tatacagtta aaccgctgat aacgttgatg
      301 taaagcgata atatagcatc tgcagcaagg aaggcttcag gaattgatat acgtttattc
      361 gctgaatcat ccaaagttcg ctcaaaccat tgtgatgatg cggttaatgc cggattaagc
      421 gtatttacga taacatatct tgaaagtgaa gcgatacgtt cacttctcat aggatttcgt
      481 ttataagcca tagcagacga gcctatttgt ttatcttcaa acggctcatc aaactccttg
      541 agatgtgaga gcagccgtat atcgtttgaa aatttgtatg cactttgtgc gatgcctgat
      601 aaaacagata aaacgtagta atcgactttt cttgaatagg tttggccgga aacggaataa
      661 accgcatcaa accccatttt tattgcaatc ttctcctcaa gctgagttgc ctttttatgg
      721 tcaccgtcaa acaatgaaag aaaactagca gatgttccgg ttgttccttt gcagcctaaa
      781 agtttaagat tgcaaagagt aaaatcgagc tgttcgaggt ctaaaagcaa atccgcaagc
      841 catagcgttg ctcttttgcc aaccgttgtc ggctgtgctg cctgaaaatg cgtataggca
      901 aggcagggta aggatttata tttatctgca aaagtccaca gcttgtcgat tgcatttaca
      961 agcaatcttt taatcaagag cattgccgac ctcataagga ttatatctgt attatcacca
     1021 acatagcagg aagtagcacc gagatgtata attggtttgg ctgaaggaca ttgctctcca
     1081 taagccagaa tatgtgccat aacatcgtgt ctgacctcgg cttcatattt attggcagct
     1141 tcataattga tgtttttaac attgcttatc atttcgtcaa tttggttttg ggttatatca
     1201 agtcccaatt ctttctgact ttcggcaagg gcaatccaca accttcgcca tgttgaaaat
     1261 ttgttatcat ctgaaaatat aaattgcatt tcagagcttg catatctttt acataatgga
     1321 ttttcgtatt taacagacat tttttaatct ccttaaaact tctctgtaac cgccaagcac
     1381 atctccaagg tcacgtctaa aacggtcctt atcaagtttt tttcctgttt ccttatccca
     1441 caaacggcag gagtcggggg atatttcatc acaaaggata attccttctc ttgttcttcc
     1501 aaactcaagc ttgaagtcca caagggttat gcctgccttg tcaaataaat cactgagcag
     1561 agcgttaatt tttaatgcag actccttcat ctcataaatt tcatccttgc ttgcaattcc
     1621 gattgcagtt atctgacttt catttatcat agggtcacca agctcatcac tctttaagct
     1681 aaattcgatt atggtgttat taagtacagt tccctcggga actccatatt tatttgagaa
     1741 actgcctgcg gcaatgtttc ttacaataac ctcaaccata acaatttcag cctttttaac
     1801 aagtacgctg cgctcatcaa ttacctttaa aagatgagtt tttataccat tcttagaaag
     1861 gtagttaaaa attatattgg aaatttcagc attaagctgt cctttctcca taagctcttc
     1921 cttctttttc ccgttaaaag ccgttgcggt atccttgaat ttgataagca ggctttcatt
     1981 atcttctcca ctgtaaacaa ttttagactt accctcatat aaaatatctt tcataactta
     2041 gaccctcttt cttcacaaat ttttaatttt acgtaccaga tatttttcaa tctaaaaagg
     2101 tgctcatcac ataatgcaat ttgggggcag tttatcaacc gacttaaaaa ctcggttttt
     2161 ttttactgct caccgcatta tctgatgaac acctttattt ttactcaatt attatgaatt
     2221 attatttatt atttgtcaag aatttttttt agctttttgc tatttttttg aa
//
LOCUS       DAOSOW010000320         2270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_47591_length_2270_cov_2.413093, whole genome shotgun sequence.
ACCESSION   DAOSOW010000320 DAOSOW010000000
VERSION     DAOSOW010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47591_length_2270_cov_2.413093"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PLD67_07870"
     CDS             complement(<1..792)
                     /locus_tag="PLD67_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014460800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HOT22478.1"
                     /translation="MTNTRIKTSWNLENSYAELPEFFYSKINLSKVSSPKLVVLNNEL
                     AEYLGLDINELQSEEGVSYFAGNSVPKGSIPIAQAYAGHQFGYFTMLGDGRAMLLGEQ
                     ITPNGKYDIQLKGSGRTPYSRGGDGRAALGPMLREYIVSEAMHALDIPTSRSLAVVTT
                     GEPVIREKILKVAILTRVASSHIRVGTFEYAANFGTEENVRQLADYTIDRHYKEIEKD
                     DFPYLSFLKEVVKRQASLIAKWQLVGFIHGVMNTDNMALSGETIDY"
     gene            complement(943..1815)
                     /locus_tag="PLD67_07875"
     CDS             complement(943..1815)
                     /locus_tag="PLD67_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HOT22479.1"
                     /translation="MMNIIQKDNKITIHDIRDFNITHIFECGQCFRWNREDDGSYTGV
                     VKNKVINVLQQENTVEFNNINTDDFDIIKNYFDFDTDYETIKNTLNTDEIMAEAIKFG
                     EGIRILNQEEWETMISFMISANNRIPMIKKVIENLSVSFGDYIGNYRGKEYFSFPTAE
                     RLSAAPVERILECKAGFRAPRIKEAATRFLYEKDKIYNIKNMSYNEGLAYLKTYKGIG
                     DKVANCILLFSMKHFDTFPVDVWVRRVMQTLYVSKETKDADIRKFAENKFGKYSGFAQ
                     QYLFFYARENGIGK"
     gene            complement(2033..>2270)
                     /locus_tag="PLD67_07880"
     CDS             complement(2033..>2270)
                     /locus_tag="PLD67_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22480.1"
                     /translation="TDIEFLNNEINLRNSYKINLEEFKKLINFAKRTSEHLYFLINDV
                     SIINSITIKCEKGSDLLIKILEEHKQLYDNAYNE"
BASE COUNT          685 a          431 c          285 g          869 t
ORIGIN      
        1 ataatcgatt gtttcaccgc ttaatgccat attatccgtg ttcataacac cgtgtataaa
       61 tccaaccagc tgccattttg caattaatga tgcctgcctt tttacaacct ctttcaaaaa
      121 actaagatat ggaaaatcat ccttttcaat ttccttatag tgtctatcaa tggtataatc
      181 agccagttgc cttacatttt cttcggttcc gaaatttgct gcatattcaa atgtaccgac
      241 acgaatatga ctgcttgcta cgcgggttaa aattgcgact ttaagtattt tttctcttat
      301 tacaggctca ccggttgtca caaccgctaa acttctgctt gtaggaatat ccagagcatg
      361 cattgcttcg cttacaatgt attcacgaag catgggacca agcgcagctc ttccgtctcc
      421 tccccgcgaa tatggagtcc tgccggagcc ttttaactga atatcatatt ttccattcgg
      481 tgtaatttgt tctcccaaga gcatagctct tccgtctcca agcattgtaa agtaaccgaa
      541 ttggtgtccg gcataggctt gagcaatggg tatggagcct tttggcacgg agtttcccgc
      601 aaaataagat actccttcct cactctgaag ctcatttata tccaaaccta aatattcagc
      661 taattcatta tttaaaacaa ctaattttgg tgagctgact ttgcttaaat ttattttgga
      721 ataaaaaaat tcaggtagct cggcataact gttttctaaa ttccagcttg tttttatcct
      781 tgtatttgtc attatgtctc caatattatc tttttatcta ttatatacgt gaaggcttct
      841 gtttaacctg tatttttatt tgcaaattta caaaatatag gttatttttt ttatagattt
      901 aaaaaaaatg cagcaaaagc tgcataaaag acttaagaca tactacttgc caatcccgtt
      961 ttcgcgagcg tagaaaaaaa gatattgctg agcaaatcct gaatatttgc cgaatttatt
     1021 ttctgcaaac tttcttatat ctgcatcctt agtttcttta cttacatata gtgtctgcat
     1081 tactcttctt acccaaacat caacaggaaa tgtgtcaaaa tgtttcatcg aaaataataa
     1141 tatgcagtta gcaaccttgt cccctattcc tttataagtt ttaagataag ccagtccttc
     1201 attatagctc atatttttaa tattataaat tttatctttt tcatacaaaa accttgttgc
     1261 tgcttcctta attctcggag ctctgaagcc tgccttacat tcaagaattc tttcaacagg
     1321 tgcagccgat aatctttctg cagtcgggaa actgaaatat tcctttcctc tataattacc
     1381 tatataatcc ccaaaactaa cacttaaatt ttcaattact ttttttatca ttggaattct
     1441 gttgtttgct gaaatcataa acgaaatcat tgtttcccat tcttcttggt ttaaaattct
     1501 tataccttca ccgaatttta tagcttccgc cattatttca tccgtattca atgtattctt
     1561 aatcgtttcg taatcggtat caaaatcaaa ataattcttt ataatatcaa aatcatcagt
     1621 atttatatta ttaaactcaa cagtattttc ttgctgcaaa acatttatca ctttattctt
     1681 aacaaccccg gtataagagc cgtcatcttc tctattccat ctaaagcatt gtccgcattc
     1741 aaaaatatga gttatattaa aatctcttat atcatgtata gtaattttgt tgtctttttg
     1801 tataatattc atcattaaca ccttaatttc tgtcttttat catttttggg ctagtcctac
     1861 aatccccatc cgtcatataa ttttataact tttaggattc atattatatt aattgtatca
     1921 taattagtca aattaataaa ttgtattatt ataaaaagaa accttgcggt ttctaatata
     1981 tcttttatgc tattaatcca aagaaaaatt tttttatttc aataaagatt gcttattcat
     2041 tataagcatt atcatatagc tgtttatgtt cttcaagtat ttttattaat aagtcagatc
     2101 ccttttcaca ttttattgtt atcgaattga ttatacttac atcattaatt aaaaaatata
     2161 aatgttcaga cgtgcgttta gcaaaattta ttaatttttt aaactcttct aggttaattt
     2221 tataggaatt tcgcaagttt atttcattat ttaaaaactc aatatcagta
//
LOCUS       DAOSOW010000321         2267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_47671_length_2267_cov_3.861664, whole genome shotgun sequence.
ACCESSION   DAOSOW010000321 DAOSOW010000000
VERSION     DAOSOW010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47671_length_2267_cov_3.861664"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="PLD67_07885"
     CDS             complement(<1..857)
                     /locus_tag="PLD67_07885"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOT22481.1"
                     /translation="MKKIYMHSLGCSKNLVDSENMLGILSKKGYKVTDYADKADYIII
                     NTCSFINDAQQESVNAILFAADIKNKVNKKTKIIVTGCLSQRFGEELSKEIPEIDIII
                     GTAGFERIDEYINEYEKNKELLIDISLELDIDKELPRNTMTEAWVSYLKIAEGCSNNC
                     TYCVIPKLRGLFKSRRIENLVEEAKVLASKGVKEIIIIAQDTSKYGIDIYGEKKLHEL
                     IRKISEIEGIRWIRIHYLYPEDIYDELINEFKNNKKLLKYFDIPMQHINDRILKRMNR
                     KTSREQITSL"
     gene            complement(865..>2267)
                     /locus_tag="PLD67_07890"
     CDS             complement(865..>2267)
                     /locus_tag="PLD67_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOT22482.1"
                     /translation="IKLLDPMPLHIRGDKEDLKKDGEKLIETLNNFNIPCNILQINKG
                     PTITRYEIQPAPGIKVSRITALSNDIALALATSEIRMEAPIPGKAAIGIEVPNRAKTS
                     VYLRSMIESREYRNAPTKIPFALGKDIAGKNIIASIDKMPHLLIAGATGSGKSVCINS
                     LIMSILFKAKPDEVKLILIDPKVVELSNYNGIPHLLIPVVTDPRKAANALNWAISEMT
                     NRYKIFAQSSVRDIDSYNEKMVTENKEKMPKIVIIIDELSDLMSVASAEVEEYITRIA
                     QLARACGMHLVIATQRPSVDVITGVIKANIPSRIAFAVSSHIDSRTILDTGGAEKLLG
                     RGDMLYHPIGLPKPVRIQGTYVSDDEIKRVVDYIKEQQIEIKMFKQNEIIKEIENQTN
                     NEEVDEYLEQAIETVVNDGQASVSYIQRKFRVGYSRAARIIDQLEERGIVGGHEGSKP
                     RKVLITKEELDKMRGN"
BASE COUNT          561 a          464 c          369 g          873 t
ORIGIN      
        1 agagatgtaa tttgttccct gcttgttttt ctgttcattc tttttaggat tctgtcattg
       61 atatgctgca ttggaatatc aaaatactta agaagctttt tattattctt aaattcattt
      121 ataagctcgt catatatatc ttccggatat aaataatgaa ttctaatcca ccggattcct
      181 tctatttctg aaatctttcg aataagctca tgaagttttt tttcaccgta aatatcaatt
      241 ccgtacttgc tcgtgtcctg agcaataatt attatttctt ttacgccctt gctggcaaga
      301 actttcgctt cctcgaccaa attttcaatt ctgcggcttt taaaaagccc tctaagcttc
      361 ggaatcacgc aataggtaca attattgctg cagccttcag ctatttttaa ataggaaacc
      421 caagcctcag tcatagtatt tcttggaagc tccttgtcaa tatcaagctc caatgatata
      481 tctataagca attctttatt cttttcgtat tcattaatat attcgtcaat tctttcaaaa
      541 ccggctgtac caatgataat gtcaatttca ggtatttctt ttgacagttc ttctccgaat
      601 ctttgagata ggcagcctgt gactattatt tttgtttttt tgtttacctt gttttttatg
      661 tctgctgcaa aaaggattgc atttaccgac tcctgctgtg catcatttat aaaggagcat
      721 gtgtttatta tgatataatc ggctttatct gcataatccg taaccttata cccttttttg
      781 gaaagtatgc ccagcatatt ttccgaatcg acaaggtttt ttgaacatcc caatgaatgc
      841 atgtagattt tcttcattta ttatttaatt tcctctcatt ttgtctaatt cttcttttgt
      901 aataaggact ttcctcggtt tacttccttc atgtcccccc acgatacctc tttcctcaag
      961 ctggtcaata attcgagcag ctcttgaata ccctactcta aattttcgtt gaatgtagga
     1021 taccgaagcc tggccatcat ttactaccgt ctcgattgcc tgctccaaat attcatcaac
     1081 ttcttcatta tttgtttggt tttctatttc tttgattatt tcgttttgtt tgaacatttt
     1141 tatttcaatt tgctgttctt taatataatc aacaactctt tttatttcgt catccgatac
     1201 atatgttcct tgtattctta cgggctttgg aaggcctatc gggtgataaa gcatatcccc
     1261 tcttccaagg agcttttcag ctccgcctgt atccaatata gttcttgaat caatatgtga
     1321 tgatacggca aatgcaatac gcgaaggtat atttgctttt ataactcctg tgataacatc
     1381 cacggaaggt ctttgcgtag caataacgag gtgcataccg caagctcttg ccagctgagc
     1441 tatacgtgta atatattctt caacctctgc cgatgctacg gacataaggt ctgacaattc
     1501 atctataatt attacgattt taggcatctt ctctttgttt tctgttacca ttttttcatt
     1561 gtatgagtct atatctctta cgctgctctg agcgaaaatc ttgtagcggt ttgtcatttc
     1621 cgaaattgcc cagttcagag cattggctgc ttttctcggg tcagttacca caggtatcaa
     1681 taaatgcgga atgccgttgt aattgcttag ctcaacaact ttcgggtcaa ttagtataag
     1741 ctttacttca tcgggttttg ctttaaataa aatactcatt attagtgaat ttatgcatac
     1801 gcttttacct gagcccgtag ctccggctat tagcaagtga ggcattttat cgatacttgc
     1861 aattatattc ttgcctgcaa tatcctttcc aagcgcaaat ggaatttttg tcggcgcatt
     1921 tctatactct cttgattcaa tcatacttct taaatacacg ctggtttttg ctctgttggg
     1981 tacttcgatt ccaattgccg ctttgccggg tatgggtgct tccatgcgta tttccgaggt
     2041 tgcgagagcc agtgctatat catttgataa tgctgttatg cggcttactt ttattcccgg
     2101 agcaggttga atttcatacc tggtaatagt cggacccttg tttatttgaa gtatattaca
     2161 tggtatatta aagttgttta atgtttcaat aagcttttca ccgtcttttt ttaaatcttc
     2221 cttatcaccc ctaatatgaa gcggcatagg gtctaatagc ttaatag
//
LOCUS       DAOSOW010000322         2256 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_48003_length_2256_cov_2.427079, whole genome shotgun sequence.
ACCESSION   DAOSOW010000322 DAOSOW010000000
VERSION     DAOSOW010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48003_length_2256_cov_2.427079"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /gene="ptsP"
                     /locus_tag="PLD67_07895"
     CDS             <1..1221
                     /gene="ptsP"
                     /locus_tag="PLD67_07895"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOT22483.1"
                     /translation="NRNIVLGFATDIGGGTSHAAIMAKGLGIPAVLGLGDISKKVKDG
                     DYIIIDGTEGKVIINPDAGLKEEYKLKINEYKKQREKFEGSAFLPAVTTDGRKLEIAG
                     NIGTPEDIESVIARGGDGVGLFRTEFLYMDRDSLPTEEEQFEEYRKAAAAFEGKPVII
                     RTLDIGGDKKLRYLELPEEMNPFLGWRAIRICLERKDIFKTQLRAILRASKYGNIMIM
                     FPMISGIGELRAAKEVLEDAKSELERENIEFNEDIKVGIMVEIPSAAITADTIAKEAD
                     FFSIGTNDLCQYSLAVDRMNPKVSKLFQPLHPGILKLIKHVIDASHRHGIFTGMCGGM
                     AGSGEAAIILLGLGLDEYSMSAASIPVIKSIIRNVSYEKAKEIASHALELETPEEVLE
                     YSRSIMKQLNIEYI"
     gene            1234..1497
                     /locus_tag="PLD67_07900"
     CDS             1234..1497
                     /locus_tag="PLD67_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOT22484.1"
                     /translation="MITEKVTILNETGLHARPASVFVNTAAKFKSDLTIQKDGKKVNA
                     KSILSVLGLGISKGTELTISAEGPDEEEAVSKLVQLIKQGINE"
     gene            1596..2117
                     /locus_tag="PLD67_07905"
     CDS             1596..2117
                     /locus_tag="PLD67_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOT22485.1"
                     /translation="MDFLELAKKRYSVRNYLPKKVEEEKLMKILEAARVAPTGANRQP
                     HRLIVVSEHAGLEKLKKAANVFGAPMAVIVCADHNISWKRHYDSKDIADIDAAIVTDH
                     MMLEATQLGLGTLWVCHFNPEVLKREFNIPENLEPVNILAIGYSSGEAESPDRHKELR
                     LPIEDFVFYESYK"
BASE COUNT          750 a          339 c          600 g          567 t
ORIGIN      
        1 aacagaaaca ttgtattggg ttttgctacg gatataggag gaggaacttc acatgcagca
       61 attatggcca agggcctggg tattccggct gtacttggtt tgggtgacat aagcaaaaaa
      121 gttaaagacg gtgattatat tataattgat gggacggaag gaaaggtaat tattaatcct
      181 gatgcagggc taaaagagga atataagctg aaaatcaacg agtataaaaa acaaagagag
      241 aagtttgaag gctcagcctt tttgcctgct gttacgacag atggaaggaa gcttgagata
      301 gcgggtaata taggaacacc ggaggatatt gaaagtgtaa tagcaagggg cggagatgga
      361 gtagggctat tcagaactga atttttatac atggacaggg actcattgcc tactgaggag
      421 gagcagtttg aggaatacag gaaagctgca gcggcatttg aaggtaagcc ggtaataata
      481 aggaccctgg atattggagg agataaaaaa cttagatatc tggaacttcc tgaggagatg
      541 aatccatttc tggggtggag ggcaataaga atttgcctgg aaaggaagga tatattcaag
      601 acgcagctga gagccattct tagagcaagc aaatatggca atataatgat tatgttcccg
      661 atgatttccg ggatcggaga gttaagggcg gcaaaggaag ttctggaaga tgcaaagtct
      721 gaattagaaa gggaaaacat tgaattcaat gaagatataa aagtaggaat aatggtagaa
      781 ataccgtctg cagcaataac tgcagataca atagctaaag aggcggattt ttttagtata
      841 ggtaccaacg atttgtgtca gtattccctg gcggtggaca gaatgaaccc taaggtgtca
      901 aagctgtttc aaccccttca ccccgggata ctgaagctta taaagcatgt aatagacgca
      961 tcccacagac atggtatatt taccggaatg tgcggaggta tggcagggag cggtgaagca
     1021 gctataatac ttttggggct gggccttgat gagtatagta tgagtgcggc gtcaatacct
     1081 gtaataaaga gtattatacg gaatgtatcc tatgaaaaag caaaggaaat agctagtcat
     1141 gcccttgagc tggagacccc ggaagaggtt cttgagtatt caaggagcat tatgaagcag
     1201 ttaaatattg aatatattta ggagagtgac attatgataa cagaaaaggt gacaatatta
     1261 aacgaaacag ggttacacgc aaggccggca tcagttttcg taaacacggc agcaaaattc
     1321 aaatccgacc tgactataca gaaggatgga aagaaagtca acgcaaagtc aattctttct
     1381 gtactgggat tgggtatttc aaaaggaact gaattgacaa tatctgctga gggaccggat
     1441 gaggaagaag ctgtaagcaa gcttgtgcag cttataaaac agggaattaa cgaatagtat
     1501 gtaacggagc actgtattct agtatgcagt gcttttgatt tgggaagaaa tagtgtagaa
     1561 tgttatataa gatgcatatt aaagaaggtg tgtttatgga ttttttagaa ctggcgaaaa
     1621 agcgatactc tgtgagaaac tatttaccaa agaaggttga agaggaaaag cttatgaaaa
     1681 tacttgaagc ggccagggta gcacctacag gagccaacag gcagccccac aggcttattg
     1741 ttgtttcgga gcatgcaggc ttggaaaaat taaagaaggc ggccaacgtg ttcggagctc
     1801 ccatggctgt tatagtgtgt gcagaccata atatatcctg gaagagacat tatgatagta
     1861 aggatatagc agatattgat gctgctattg taactgacca tatgatgctt gaagccacac
     1921 agctgggctt gggaactcta tgggtatgtc attttaatcc cgaggtgttg aaaagagagt
     1981 ttaatattcc ggaaaacctc gaaccggtaa atattctcgc tataggttat tcttcgggtg
     2041 aggcagaatc tccagacagg cataaagagc ttagattgcc cattgaagat tttgtattct
     2101 acgaatcgta taaataatag atagcggcgg aaaaaacaag cgtaaaggcg agtgaggcag
     2161 agttgagatt tttttcctat tttatgaaag gaatattgat agggataggc ggcgttgctc
     2221 ccggtgtaag cggaggagca attgccgtaa tatttg
//
LOCUS       DAOSOW010000323         2245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_48283_length_2245_cov_2.528311, whole genome shotgun sequence.
ACCESSION   DAOSOW010000323 DAOSOW010000000
VERSION     DAOSOW010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48283_length_2245_cov_2.528311"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..>2245
                     /locus_tag="PLD67_07910"
     CDS             226..>2245
                     /locus_tag="PLD67_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HOT22486.1"
                     /translation="MADNFGLKIGIEGEREFKKALSDINQSFKVLGSEMNLVSSQFDK
                     QDKSIQAITARNNALNKEIDAQKDKISALEAALKNASESFGENDRRTKNWAIQLNNAK
                     AELNNMEKELEESAEEADKLGKELEESGKSADNASSKFEKLGGVLKGIGAAMASVAAA
                     AGAATIKLGKEIIQQFGELEQNLGGSEAVFGEYALSIQKTGEEAYKNMGVSQSQYLAT
                     ANKMGALFQGSGIEQQKSLELTEKAMQRAADMASVMGIDMQMALDSVAGAAKGNFTMM
                     DNLGVAMNATNIQAYALAKGLDFTWASATQAEKAEVAMQMFFENTEQYAGNFAKESTE
                     TITGSIGLMQAALGSFTAGLGNADADMKNLTNNLVDAFQAVIKNIVPVLENIVTALPA
                     AMDAIISAIGDILPVLLSTVTELFNQVFETILNLLPELIPAAVDAVMTIVETLINNLP
                     LLIDTAVLLITALVEGMGLALPELIPAAVSAVITIVQSLMENMEMVLEAAFAIIKGLA
                     EGLLNALPALIEALPQIITTIINFITNNLPAIIGMGVELIVQLAFGLIKAIPQLVAAL
                     PQIVTAIISGIGKAAVSIVEVGKNIVVGLWDGIASMMGWIKEKISGFVGGIVSNVKGV
                     LGIQSPSTVFAGIGTNMSLGLGEGFSKAMNSVKDDMNRSIPTSFDIDAN"
BASE COUNT          741 a          389 c          492 g          623 t
ORIGIN      
        1 gaaggtgatt tcaatataga agcaaatttg ataaaacaat actgtccatt attaaatatt
       61 acacataacc ctaaaaaact acaagaactc aaggaagaca gacaaaattg taaaagaata
      121 gcaagaggag aacattaatt atttaattat ctattgcata ataacaagtc aatggcactc
      181 taacaagggt gtctttttta tgctcaaaaa caaggaggtg gaagtatggc agataacttt
      241 ggtctcaaga ttggcattga aggcgaacga gaatttaaga aagctttaag cgatataaac
      301 caatcattca aggttttagg ttctgaaatg aatttagtat catctcagtt tgataaacag
      361 gataaatcaa tacaggctat aacagcaagg aacaatgctc ttaacaaaga aattgatgct
      421 caaaaggata aaattagtgc ccttgaggct gcccttaaga atgcctccga gagctttggt
      481 gaaaatgacc gaagaactaa gaattgggct atccagctta acaatgctaa agcagaactt
      541 aataacatgg aaaaagagct tgaagaatct gctgaagaag ccgataaact tggtaaggag
      601 ttagaagagt ccggtaaatc tgcagataat gcaagcagta aatttgaaaa attaggcggt
      661 gttcttaaag gcatcggtgc tgctatggct tctgtagctg cagccgctgg ggcagcaaca
      721 ataaagcttg gcaaggaaat tattcagcaa tttggtgaat tagaacagaa ccttggaggc
      781 tcggaagcag tatttggaga gtatgcttta tcaattcaaa aaaccggtga ggaagcctac
      841 aaaaacatgg gtgtatccca aagtcaatat cttgctactg ccaacaagat gggtgctctc
      901 tttcaaggtt ctgggattga acaacagaag agcttggaac ttacagagaa ggccatgcag
      961 cgagctgccg atatggcatc tgtaatgggc attgacatgc aaatggcact tgattctgta
     1021 gcgggtgcag ctaaaggtaa cttcaccatg atggataact taggtgttgc tatgaatgcc
     1081 acaaacattc aggcttatgc tcttgcaaaa ggacttgatt tcacttgggc atctgccaca
     1141 caagcagaaa aagcggaagt agcaatgcag atgttctttg aaaatacaga gcaatatgca
     1201 ggcaactttg ccaaggaatc aactgaaaca ataaccggtt caatcggact catgcaggca
     1261 gcccttggtt cttttactgc aggtcttggc aatgccgatg cagacatgaa aaatttaacc
     1321 aataatcttg tagatgcatt tcaagcagtt attaaaaaca ttgtgcctgt cttggagaat
     1381 attgtaaccg cactgcctgc tgcaatggat gcaataatat cagctattgg agatattctc
     1441 ccggtgcttc ttagtaccgt tacagagtta ttcaatcagg tatttgaaac aatcctgaat
     1501 cttctaccag agcttatacc ggctgccgtt gacgctgtta tgacaattgt tgaaacactc
     1561 ataaataact tacctttatt aattgatacg gctgtacttt taattaccgc tcttgttgaa
     1621 ggtatgggac ttgcattacc tgaattaatt cctgcagcag taagtgctgt aataacaata
     1681 gtacaaagtc ttatggagaa tatggagatg gttttagaag cagctttcgc tatcataaag
     1741 ggattagcag agggacttct taatgcccta cctgctctaa ttgaagcact gccgcaaatc
     1801 ataacaacca ttatcaactt cattacaaat aacctgcctg caattatcgg tatgggtgtt
     1861 gagctgatag tccagcttgc ttttggttta attaaggcaa tacctcaact tgtggctgca
     1921 ttgccccaaa tcgtaacagc aataatttca ggtattggaa aggctgctgt ttcaatagtt
     1981 gaagtaggaa aaaatatagt tgttgggcta tgggatggta ttgcttccat gatgggttgg
     2041 ataaaggaaa aaatcagtgg ttttgtaggt ggaatagtat ctaatgtaaa aggtgtttta
     2101 ggcattcaat ccccttctac tgtatttgca gggattggaa ctaatatgag cttaggcctt
     2161 ggcgagggtt tctctaaggc aatgaattca gttaaagatg atatgaacag gtcaattccg
     2221 acaagctttg atatagatgc gaatt
//
LOCUS       DAOSOW010000324         2227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_48786_length_2227_cov_3.075967, whole genome shotgun sequence.
ACCESSION   DAOSOW010000324 DAOSOW010000000
VERSION     DAOSOW010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48786_length_2227_cov_3.075967"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLD67_07915"
     CDS             <1..186
                     /locus_tag="PLD67_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22487.1"
                     /translation="VKIASFNISIILMYFTAKLFINLPIRWFMIIAAQVLFIIYDYAY
                     TIFINQYINFIKPKLGR"
     gene            410..2182
                     /locus_tag="PLD67_07920"
     CDS             410..2182
                     /locus_tag="PLD67_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOT22488.1"
                     /translation="MLKKIKYAILLLLLTVLLTSCTESADADANTPPSDETNQEIPEI
                     IESELSEEEKAKLELEKRIAEREELEEQRKEELGEFYVPLIPIGEEREKKSVEVKALY
                     VTGHTAGNDLNKENIDAYGEYVKALAENNTQKARELFDRAEKANKFERIVGIALATEI
                     NSLVINVKDDNGFITYKSNIEIVKDMNKETPIPIKDIDNMLKILKEYDLYIIGRIVAF
                     KDKNFAVKSPEHAIQLKSGGIWLDPNSGKIPWINPFDKYVWDYNVAIAKEASLLGFDE
                     IQFDYVRFPDGAKTYNLIAEFPGRDGRDKDEAIADFLEYSRKELEPYSVNLGADVFGL
                     VTRTWDDYPEDIGQTWILIGKHVDNICPMVYPSHYSTGWYNLENPNAHPYLVVKGAME
                     EAIEKNSSMETPPIIRPWIQDFDWDGIAYGPDKVRDQIIAAKELGIYEYMIWNPSNVY
                     DPYAFMVTEKEKITAYPLNKGELDYREKSPADAAVKYLTGMLQDRHSYTYLMTPVSDR
                     VNDFDEFVKLREEDNIKLLSYKVYGYEMDANDSNKANVSLSYKIEVKNGDTTEIIEKD
                     NAKWQAIRELNIWKVKVNWDGDIS"
BASE COUNT          834 a          317 c          450 g          626 t
ORIGIN      
        1 gtaaaaatag catcttttaa catttctatc atattaatgt actttactgc aaaactattt
       61 ataaacttac ctatccgatg gtttatgata atagcagccc aagtattgtt tattatctac
      121 gactacgcct atacaatatt cataaatcaa tatataaatt ttataaaacc aaagttggga
      181 agatagaagc acttctcatt tttaagattg aattctgaga aatggttgac atatctttcg
      241 taaacgagca acaattttgt gtgacgtaag gagtcgaaaa atgaaaaaac ctgtattatc
      301 cattgcttat aggtatagat tatgttaaaa ttaatcgtgg tataagagta gtttataaat
      361 attatatact atgggtcatc tgcacctatt atttaaaatt ggaggattta tgcttaaaaa
      421 aataaaatat gcaattttat tattattact aaccgtgtta ttgacatctt gtactgaatc
      481 cgcagatgca gatgcaaata cacccccttc cgatgaaaca aatcaggaaa ttccggaaat
      541 aatagaatcc gaattatcag aagaagaaaa agcaaaatta gagttggaaa aaagaattgc
      601 tgaaagggaa gaattagaag aacaaaggaa agaagagctg ggagaatttt atgttccgct
      661 gattcccata ggggaagaaa gagaaaaaaa gagtgttgaa gttaaagcat tgtatgttac
      721 cggtcatacg gcaggcaatg atttaaacaa ggaaaatatc gatgcatatg gtgaatatgt
      781 gaaagcactt gcagaaaata acactcaaaa agcaagggaa ttgtttgaca gggctgaaaa
      841 ggccaataaa tttgagcgta ttgtgggaat agctcttgct actgagataa acagtctggt
      901 tataaatgtt aaagatgaca acggctttat aacatataag agcaatattg aaattgtcaa
      961 agatatgaac aaggaaacac ctattccgat aaaagatata gacaatatgc ttaaaatatt
     1021 gaaggaatat gatttataca tcataggaag aattgttgca tttaaggaca agaattttgc
     1081 cgttaagagt ccggagcatg ccatacagct taaatccggc gggatatggc ttgaccctaa
     1141 cagcggaaaa attccgtgga taaatccgtt tgataaatat gtgtgggatt ataatgtggc
     1201 aattgccaaa gaagcatcac tgctgggttt tgatgaaatt caatttgact acgtgcgttt
     1261 tccggatgga gctaaaacat acaatcttat tgcggaattt ccgggaagag acggcagaga
     1321 taaagatgaa gcaatagctg attttttaga atattcgaga aaagaattag agccttacag
     1381 tgtaaattta ggcgcagatg tatttggtct cgttacaagg acatgggatg attatcctga
     1441 ggatataggc cagacttgga tactgattgg taaacatgtt gataatattt gtcccatggt
     1501 ttacccgagt cattattcaa cagggtggta taatttagaa aatcctaatg ctcatcctta
     1561 cttggttgta aaaggtgcta tggaagaagc aatagaaaaa aattcctcca tggaaacacc
     1621 tccaattata cgaccttgga ttcaggactt tgactgggat ggaattgcat acggtcctga
     1681 caaagtaaga gaccagataa tagcagcaaa ggagcttgga atatatgaat atatgatatg
     1741 gaatccaagc aatgtttatg atccatatgc attcatggtt acagaaaagg aaaagataac
     1801 cgcttatccg ttaaataaag gagaactgga ctacagagaa aaatcacctg ctgatgcagc
     1861 agttaagtat ttaaccggta tgcttcaaga caggcacagc tacacatatt taatgactcc
     1921 tgtttcagac agagttaatg attttgatga atttgtaaaa cttcgggaag aagataatat
     1981 taagctttta agctacaagg tttacggcta tgaaatggat gcaaatgaca gcaataaggc
     2041 caatgtaagc ctatcctata aaatcgaagt taaaaacgga gatacaacag aaataataga
     2101 aaaagacaat gctaaatggc aagcaataag agagcttaac atctggaaag taaaagtaaa
     2161 ctgggatggt gacatatctt aactgtttcg aaaggattaa tatcacggta atacatttta
     2221 taatgct
//
LOCUS       DAOSOW010000325         2221 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_48987_length_2221_cov_3.872114, whole genome shotgun sequence.
ACCESSION   DAOSOW010000325 DAOSOW010000000
VERSION     DAOSOW010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48987_length_2221_cov_3.872114"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..804)
                     /gene="cysE"
                     /locus_tag="PLD67_07925"
     CDS             complement(247..804)
                     /gene="cysE"
                     /locus_tag="PLD67_07925"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOT22489.1"
                     /translation="MIKFIKSIIEDIDSVFDRDPAARSRMEVLLLYPHIKALIAYKIS
                     HKLYLKRRYFLARLISNIARHRTGIEIHPGATIGKGLFIDHGMGVVIGETAVVGNNVT
                     MFHGATLGGTGNEKGKRHPTVGDNVVIGASAKILGNIYIASGTKVGANAVVLNDTNPN
                     STLVGIPAREVKKGNKIINLSEYAI"
     gene            complement(822..1706)
                     /gene="cysK"
                     /locus_tag="PLD67_07930"
     CDS             complement(822..1706)
                     /gene="cysK"
                     /locus_tag="PLD67_07930"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOT22490.1"
                     /translation="MINTLDLIGNTPVIKLPGENIYLKMEKFNPGGSVKDRAALGMIE
                     DAEAKGYLNKNSIIVEPTSGNTGIALALIGRLKGYKVIIVMPETMSMERRNIIEAHGA
                     TLILTEGQKGMSGAIARAHELLKDDNNYFMPDQFNNPANPRIHYDTTGQEIIRQLNDI
                     DIFTAGVGTGGTLTGVSKRLKEYNKNIITAGVEPSKSPVLSGGSPSPHKIQGIGAGFV
                     PGTYNGSFVDEIITITDEEAIDTAVEVSAKIGMLIGISSGANVAAARKLAQKYGSNKK
                     ILTISPDGGEKYLSVVKY"
     misc_binding    complement(1935..2163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          580 a          498 c          367 g          776 t
ORIGIN      
        1 cggagttcag gaagtatatt taacaattga cggaggtcct tacgaatcag gacatataag
       61 aatcgaagaa ggtgagccct tattggtaga ctacgataat gttaacacga gagaataaaa
      121 tatggtttac ataattgaac attatgtaaa ccattgggga cacgcatatc cccctcatta
      181 cggggacatt ccttcaataa ttatctcaca aagaatcact gtatattgaa ggtgtgtccc
      241 catgcattat attgcatatt cgctcaagtt aattattttg ttcccttttt taacctctct
      301 tgcaggaatc ccgacaagag tagaattagg gttggtatca ttcaatacga cggcatttgc
      361 tcctaccttg gttcctgaag caatatagat gtttccaagt atctttgccg atgctcctat
      421 tactacatta tccccgacag taggatgtct tttccctttt tcatttccgg tacctcccaa
      481 tgtagctcca tggaacattg tcacattgtt ccctacaacg gctgtttctc ctattacaac
      541 acccataccg tggtctataa aaagtccttt accgatggtt gcgccgggat gtatttcaat
      601 tcctgtcctg tgccttgcaa tatttgatat aagtcttgcc aaaaaatatc ttctttttaa
      661 atataattta tgagaaattt tatatgctat caaggcttta atatgaggat acaaaagtaa
      721 gacttccatc ctgcttcttg cggcagggtc cctgtcaaac acagaatcta tatcttcaat
      781 tattgatttt ataaatttaa tcatgatatc attcctttca attaatactt aactaccgac
      841 aaatactttt ctccgccgtc cggagatatt gtaagtattt ttttattgct gccgtatttt
      901 tgcgctaatt ttcttgctgc cgcaacatta gctcctgacg aaattcctat gagcattcca
      961 atttttgctg aaacttctac agcagtatcg attgcttcct catcggttat tgttattatt
     1021 tcatctacaa aacttccatt gtatgttccc ggtacaaagc ctgcccctat tccctgaatt
     1081 ttatgaggtg acgggctgcc gcctgataat acaggtgact ttgaaggctc aactcctgca
     1141 gtaattatgt ttttgttata ttctttcagc cttttcgaaa ctccggttag agttcctccg
     1201 gtaccgactc cggctgtgaa tatatcaata tcgtttagct gcctaatgat ttcctggcct
     1261 gtagtatcat agtgaatcct ggggtttgca ggattattaa actggtcagg cataaaataa
     1321 ttattatcat ctttaagcag ctcatgagcc cttgctattg ctccgctcat tcctttttgt
     1381 ccttccgtaa gtatcaatgt ggcaccatgt gcttcgatta tgtttcttct ttccatactc
     1441 atagtttcag gcataacgat tattacttta tatcctttca atcttcctat tagagccagt
     1501 gcaatacctg tatttccgct ggtaggctcc acaatgatgc tgtttttatt taaatatccc
     1561 ttcgcttcag catcttcaat catccctaat gcggctctgt ccttaacact gccgccggga
     1621 ttgaattttt ccatttttaa ataaatattt tctcccggta atttaatcac aggagtgttt
     1681 cctattaaat ctaatgtatt aatcatatta ttacctcttt cttttccact aaatcgataa
     1741 tcaatatagc tacaaacgga atatatatgc ataagtggta ttcgcaggtt aggacatatc
     1801 gcagtctcgt tcaccaaaat atctgctccc tttggtcgca tattttggga actcgttgcg
     1861 gttggcctac cgccatatgt ttctcgctgt cgctcgaaca tatggggtta ggacatacaa
     1921 aaaatccgcc tctgcaaaga gacggattga aatccgcggt tccactctaa ttggctctat
     1981 agccccctta tatagcacca tcatgctact ccactataac ggatggttcc gcagcagcct
     2041 acttaattcg gtgctgtact ccaaagggca cttcacatat acttccaata agcatccttc
     2101 cagccttgag atgctctctc tgtaaattcg ttatatgcta ctttcctttt tcgccgtatt
     2161 tcctactaat ttagttgggt atattttctt tattatatta tactgcttta taaaatgcaa
     2221 c
//
LOCUS       DAOSOW010000326         2218 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_49101_length_2218_cov_2.895515, whole genome shotgun sequence.
ACCESSION   DAOSOW010000326 DAOSOW010000000
VERSION     DAOSOW010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49101_length_2218_cov_2.895515"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PLD67_07935"
     CDS             complement(<1..272)
                     /locus_tag="PLD67_07935"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019379857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HOT22491.1"
                     /translation="MKKRIAFYILVFMCLLVNFNIVYATPAILSESAVLIDASSGTIL
                     AQKNADKKMYPASLTKIMTAILAIELGELTDVITVDDDTPFEIEGS"
     gene            577..1146
                     /locus_tag="PLD67_07940"
     CDS             577..1146
                     /locus_tag="PLD67_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOT22492.1"
                     /translation="MNNSIYKKGLAVVLIIVIIIMAVLGIKLSGKEKEVFFADYSNTN
                     EENEEPKETERYIFVDIDGAVVSPGVYKLIEGSRVIDAINLAGGLKEKAFTQNLNKAR
                     KLVDGEKIYIYLEGDEEAERLHPNNLINLNTASVDVLMSLPGIGEVYANRIIDYRNKK
                     QFSSIEEIKNIEGIGDKTFEKLKELITVN"
     gene            1233..>2218
                     /gene="selA"
                     /locus_tag="PLD67_07945"
     CDS             1233..>2218
                     /gene="selA"
                     /locus_tag="PLD67_07945"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOT22493.1"
                     /translation="MDANKYYRNIPKVDSIMENEKIKELLKITSRESVLECARRATEE
                     LRIFINDNPNNEERINAEIKNIINKITSLVHNLLAFNMKKVVNGTGTILHTNLGRAPI
                     SKDAAKRAMDIVTGYSNLEFDLELGKRGSRYSHFEKIISKITGAEAAMAVNNNAAAVL
                     LILSSLAKDKEVIVSRGELVEIGGSFRIPDVMKQSGVNLIEVGTTNKTRLHDYEEKIS
                     SETAALLKVHTSNYKIVGFTESVSVEELSDLGKKYNIPVIEDIGSGVLADLSGYGLTY
                     EPTVQNSIKAGVDIVCFSGDKLLGGPQAGIIAGKKQYIDKIKKHPLNRALRI"
BASE COUNT          821 a          308 c          460 g          629 t
ORIGIN      
        1 tggcttcctt cgatttcaaa gggagtatca tcatccacag taataacatc agttaactct
       61 cctaattcta tggcgaggat tgccgtcata atcttggtta aacttgccgg atacattttt
      121 ttgtccgcgt ttttttgcgc caaaatagta ccggaggatg catcgattaa aactgctgat
      181 tcgctgagta tcgcaggtgt cgcatataca atgttgaaat taaccaacaa acacataaag
      241 actaatatat agaatgctat tcgttttttc atttgtaact ccttgtttat attgtatgga
      301 tataccttca ttaagggtaa gtctttgggg tcaagctatg aaggaatgtc cccaaaacag
      361 gtgtcacctt tacaggatag tctaaggggc aagagaagta tccccgattg ggatatttat
      421 ggttatcgat tgccccatat aaatcaaata atagtatagc agaaaaatta atatttgtaa
      481 tcaaaaattt tgatttttta gattaataat gtataatata ggaaagggta gctacccgca
      541 aatgaagggg atatatttat aatactaggg ggtattatga acaacagcat ctataagaaa
      601 ggacttgccg ttgttttaat cattgttata attattatgg ctgttttggg aattaaactt
      661 tccggcaagg aaaaagaagt tttttttgca gactattcaa atacaaatga agaaaatgaa
      721 gaaccaaaag agacagaaag atatattttt gttgacattg acggagccgt ggttagtccc
      781 ggggtttata agttaattga aggaagcagg gttattgatg caattaattt agcaggaggc
      841 cttaaggaaa aagcatttac gcaaaattta aacaaggcga ggaaactggt tgatggagaa
      901 aagatttaca tatatttaga aggcgatgag gaagccgaaa gattacatcc caataatctg
      961 attaatctca acactgcttc agttgatgtt ttaatgtctc ttcccggaat cggagaagtt
     1021 tatgcaaata gaattattga ttacagaaat aaaaaacaat tcagttccat tgaagaaatt
     1081 aaaaatatcg aaggaatcgg ggataaaact tttgaaaagt taaaagagtt aataacggta
     1141 aattgaggca ttcaccaacg ggatacatag attgacaaat ctaaattaat gataagtaac
     1201 cggtttgata aattaaatat aggagttaga aaatggatgc aaataagtat tataggaaca
     1261 ttcctaaggt agattcaata atggaaaatg aaaaaattaa ggaattatta aaaattacaa
     1321 gcagagaatc ggttttggaa tgtgcaagaa gagctactga agagttaaga atctttataa
     1381 atgacaaccc aaataatgaa gaaagaataa atgcagaaat aaaaaatata ataaataaaa
     1441 ttacttcact ggtacataat ctgctcgcct ttaatatgaa aaaagttgta aacggaaccg
     1501 ggacaattct tcatacaaat ttgggaagag caccgatatc aaaagatgca gcaaaaagag
     1561 ctatggatat tgtaacagga tattcaaacc ttgaatttga tttagaatta gggaaaagag
     1621 gctccagata ttcgcatttt gaaaaaataa tctcgaaaat taccggggca gaagcagcaa
     1681 tggcggtgaa taacaacgca gctgccgtgc ttttgatttt gtcttcattg gctaaggata
     1741 aggaagtaat tgtatcaaga ggggaactgg tagaaatagg aggctccttt agaataccgg
     1801 atgtaatgaa acaaagcggc gtgaatttaa ttgaagtagg caccacaaat aaaacccgct
     1861 tacatgatta tgaagagaaa atttcttccg aaactgcagc tttattaaag gtgcatacca
     1921 gcaattataa aattgtgggt tttacggaaa gtgtttcagt cgaggaatta tccgatttgg
     1981 gtaaaaaata taatattcct gtcattgaag atatcgggag cggagtgttg gctgatttaa
     2041 gcggttacgg tttaacatat gagcccacgg tacaaaactc aataaaggca ggagttgaca
     2101 tagtatgttt cagcggcgac aaacttttgg gaggtcctca ggcaggcatt attgcaggga
     2161 aaaaacaata tatagataaa ataaaaaaac atcctttaaa tagggccttg agaatcga
//
LOCUS       DAOSOW010000327         2215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_49169_length_2215_cov_3.817130, whole genome shotgun sequence.
ACCESSION   DAOSOW010000327 DAOSOW010000000
VERSION     DAOSOW010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49169_length_2215_cov_3.817130"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PLD67_07950"
     CDS             complement(<1..911)
                     /locus_tag="PLD67_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOT22494.1"
                     /translation="MAKTTTNKTNKTNKKTSINKKSTSSKASGKNILIKEITGIFIIS
                     FAVFMIIVATKEAQTGIIGKSINTFTHSAFGAGAKVLPYFILAVGILRLLNIIILNDN
                     NQIAAIIGFFLCFIIFKAISDADVYAHMADSNDFQELIKASAEFGMRNKGGGVFGNAI
                     TFLFVKLIGKNGTYVALGALIFSSIVLLTNQSIIGILKLIKNYIQKFTNLIYRFIFTE
                     SDESKTKKEKPAKKQQEESIQIYDYMSAVQENKKAEITQLKIEDIQKNENIPEASKDV
                     PIEESKEKFKQKEITDEIITISDVKTD"
     gene            complement(1043..1828)
                     /locus_tag="PLD67_07955"
     CDS             complement(1043..1828)
                     /locus_tag="PLD67_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HOT22495.1"
                     /translation="MENNKMNIPQQKTQPSLNQAQSSTEPINPFIFSGTPNDNEDGSQ
                     PIENVKQVGSITQVEPVKDICFISIIGEIEGHSSLPPQSKTTKYEHIIPMIIGAEMDP
                     QIKGILLLINTMGGDVEAGLAISELIASITKPTVSIVLGGGHSIGTALAVSADYSFIA
                     PTGTMTIHPIRTSGLVIGVPQTFRYFQKMQERITNFIVSNSHVDKQALLDYMYDTDEI
                     ANDVGTVLNADEVVNIGLIDEIGGFSKAMKKLRELIDNMEEEG"
     gene            complement(1910..2143)
                     /locus_tag="PLD67_07960"
     CDS             complement(1910..2143)
                     /locus_tag="PLD67_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HOT22496.1"
                     /translation="MKLSELYSKEIINVDTGENLGIFGDCDLVIDEKTGEIISFISGG
                     NSPFAFFKEPKRKEILWKNIKKIGSDMIIIEND"
BASE COUNT          604 a          412 c          331 g          868 t
ORIGIN      
        1 ttgtctgttt taacatccga tattgtaata atttcatcag ttatttcttt ttgcttgaat
       61 ttttccttag attcttcaat cggcacatct tttgatgctt caggaatatt ttcgtttttt
      121 tgaatatctt ctatttttaa ttgagtaatt tcagcttttt tattttcttg aacagcactc
      181 atataatcat aaatttgtat gctttcttct tgctgttttt tagcaggttt ttctttttta
      241 gtcttgcttt cgtcactttc cgtaaatatg aatctatata taaggtttgt aaatttttgt
      301 atgtaatttt taatcaattt caatatgccg attatacttt gatttgtaag aagaactata
      361 gatgaaaaga taagtgcgcc caaggctaca tatgttccgt ttttaccgat aagctttaca
      421 aataaaaatg taatcgcatt tccaaaaact cctccgcctt tgttacgcat tccgaactct
      481 gcagaagctt taatcaattc ctgaaagtcg ttgctgtcag ccatatgagc atatacatcc
      541 gcatcggata ttgccttgaa gattatgaaa caaagaaaaa atccgataat tgcggctatt
      601 tgattattat cattcaatat aataatattt aaaagtctca aaatccctac agctaatata
      661 aaatacggca ataccttagc gcctgccccg aatgcggaat gagtaaaggt atttatactt
      721 ttgccgatta taccggtttg ggcttcctta gttgctacga taatcatgaa aacagcaaat
      781 gatataataa aaataccggt tatttcttta attaaaatat ttttgccgga tgctttagag
      841 cttgtactct ttttatttat gcttgttttt ttatttgttt tattagtttt atttgtggtt
      901 gttttagcca tttaaagcaa tttccccctg tttctttgta aatttaatta tatcatagga
      961 tattatcatt ccgcaatggt aatttgtcga tatcctaatg attggggaca tttctctttt
     1021 tatttacaat gtccccttat ttttaaccct cttcttccat attgtcgata agctctctca
     1081 gctttttcat tgctttgctg aatccgccta tttcgtcaat caatccaata tttacaactt
     1141 catcagcgtt taataccgtt cctacatcat tggctatttc atccgtatca tacatataat
     1201 ctaataaggc ttgtttgtca acatgagaat ttgatacgat aaaattagta atcctttcct
     1261 gcattttctg gaaatacctg aaagtttgag gtactcctat tactaagccg ctggtacgta
     1321 tcggatgaat tgtcatcgtg cctgtaggtg caatgaaaga gtaatcggca gaaactgcta
     1381 aggctgttcc aatgctgtgt ccccctccta atacaattga aaccgtaggt ttcgttatgc
     1441 ttgctatcaa ttctgaaatg gcaagaccgg cttcaacatc tccacccatc gtattaatca
     1501 gcaataaaat accttttatc tgagggtcca tttcagctcc tattatcata ggaattatat
     1561 gctcatactt tgttgttttg ctttgaggag gcaatgaaga gtgtccttca atttctccga
     1621 ttatacttat aaaacaaata tcttttaccg gttccacttg agttatgctt ccaacctgtt
     1681 taacattttc tataggctgg ctgccatctt cattatcatt aggtgtaccg ctgaatataa
     1741 aggggtttat cggctctgtt gaggattgag cttggttaag tgatggctgt gtcttctgtt
     1801 gcgggatatt catcttgtta ttttccatag ttcctccata aaaaaataat caggtattcc
     1861 tgattattct ttgaagtttt attaaaaata tacactgaca tcatgattgt cagtcatttt
     1921 ctatgataat catgtcgctt ccgatttttt ttatattttt ccacagtatt tcttttcttt
     1981 ttggttcttt gaaaaatgca aaaggtgaat tgccgccgga tataaagctg attatttcgc
     2041 ctgttttttc atctataacc aagtcacagt caccgaaaat tcccaaattt tctccggtgt
     2101 caacattaat aatttctttg ctgtataact cgcttaattt catatattcc cccccatttc
     2161 tatttcttat tcaattataa tgttaatgaa ttcctgtatg accgaaacct ccctg
//
LOCUS       DAOSOW010000328         2205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_49456_length_2205_cov_4.132558, whole genome shotgun sequence.
ACCESSION   DAOSOW010000328 DAOSOW010000000
VERSION     DAOSOW010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49456_length_2205_cov_4.132558"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLD67_07965"
     CDS             <1..261
                     /locus_tag="PLD67_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22497.1"
                     /translation="NKVPLVVNENLIYIYVNVRKPMFNHDAAFGLFDVNSIENIYDDD
                     GKAVIEMKSGKMIKTRQSVKSLKKSILNARIAKEIYKEKHNK"
     gene            351..623
                     /gene="yabP"
                     /locus_tag="PLD67_07970"
     CDS             351..623
                     /gene="yabP"
                     /locus_tag="PLD67_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HOT22498.1"
                     /translation="MDEKIMQSLVLENKEILNVTGVEGVDNFNDETVVLITTKGRLTI
                     KGENLAISKLNVDEGKLMVKGIINSLVFSEYEGQREKVSLVKRLFK"
     gene            625..1188
                     /locus_tag="PLD67_07975"
     CDS             625..1188
                     /locus_tag="PLD67_07975"
                     /inference="COORDINATES: protein motif:HMM:NF021118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HOT22499.1"
                     /translation="MDYLPYSQEYMLAVSIMAGMFLGFIWDIYRLIRHYGKFKTTGTV
                     IGDIIYWIVSITISTRLILDISYGNVRFFILLGFLAGALLYFYGISSYVLKLFIFIID
                     SIIKVIKTVLHLLIDPIKFIIRKIEIFLYPLKLKYHKVEEKLKKRYKFLKFKLKKVFK
                     NKKMIYNRKKRKRQLKKRKKRRLSPGA"
     gene            1238..1696
                     /locus_tag="PLD67_07980"
     CDS             1238..1696
                     /locus_tag="PLD67_07980"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOT22500.1"
                     /translation="MNEDQRIIELKKKINHYDFREKEREIKEQKRIKKLAAPIKKKRR
                     FNVINFLFLIFVIYFAFTAFNQYEMLLDLNGQIKEKEAIKAEAEKEALELKSDVEKLN
                     EEETLMEIIEKIARDQYKMVKPNETIYIDKNKNDNKLIQGIGSQKDLINE"
     gene            1774..>2205
                     /locus_tag="PLD67_07985"
     CDS             1774..>2205
                     /locus_tag="PLD67_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22501.1"
                     /translation="MIGRTQDEKYDLIITNTLKKELKHIKENWLLLLVCFFVSRQNII
                     NEIFPFAIVVLSSYCHIKGPWISILFVCIAAILSVRFDFVYIIMLTAIYGYYFNFRNE
                     GKKSILLISGYSAAVLFFSKTAILVSEGFNTNEFMLNVFETM"
BASE COUNT          857 a          279 c          404 g          665 t
ORIGIN      
        1 aataaggtgc cactggttgt aaatgaaaat cttatttata tatacgtcaa tgttagaaaa
       61 cctatgttta atcatgatgc tgccttcgga ttatttgatg taaattctat agaaaatatt
      121 tatgacgatg acggtaaggc ggtaatagaa atgaagtcag gtaaaatgat taaaacgaga
      181 caaagtgtta aatctttgaa aaaaagtatt cttaatgcaa gaattgcaaa ggaaatatac
      241 aaagaaaaac ataacaaatg agactgcgag atgacagtac ccccgtcccc ttgtcatctg
      301 tcataaataa aattccatgg acatataata tatttgaggg gggaatgttc atggatgaaa
      361 aaattatgca aagtttagta cttgaaaata aagaaatatt aaatgtcacc ggtgttgaag
      421 gagtagataa cttcaatgac gaaacagttg tgcttatcac tacaaagggc agacttacca
      481 taaagggaga aaatttagcc ataagcaagc taaatgttga tgaaggcaaa cttatggtaa
      541 aaggtataat aaattctctg gttttctctg aatatgaagg acagagagaa aaagtgagct
      601 tagtaaagag actgttcaaa taatatggac tatttacctt attctcagga atacatgctt
      661 gcagtttcaa taatggcagg tatgtttctt ggttttatat gggatattta caggcttata
      721 aggcattatg gcaagtttaa aacaacagga actgttatag gtgacattat atattggata
      781 gtcagcataa ctataagcac ccggttaatc ttagacatca gttacggcaa tgtcagattt
      841 tttattcttt tgggattttt agcgggtgct ctcttgtatt tctacggaat cagcagttat
      901 gttttaaagc ttttcatatt tattattgac tctattataa aagttataaa aacggtttta
      961 catcttttaa tagacccgat taagtttata attagaaaaa tagagatttt tttgtatcct
     1021 ttgaagctaa aataccataa agttgaagaa aaactcaaaa aaagatataa attcttgaaa
     1081 tttaaattaa aaaaggtttt caaaaataaa aaaatgatat ataataggaa aaaacggaag
     1141 aggcaattaa aaaaacgaaa aaaaagaaga ttgtcgccag gagcctaaga taaggttgcc
     1201 ctaacctgta aactacaaaa gaaaggagta gtctcagatg aacgaagacc aaagaatcat
     1261 agaattgaag aaaaagatta atcattatga ttttcgtgaa aaagaacgag aaataaaaga
     1321 acaaaagaga attaaaaagc tggctgctcc aataaagaaa aaaagaagat tcaatgtaat
     1381 taattttctg tttttaatat ttgttatata ttttgcattc actgctttta atcaatatga
     1441 aatgcttttg gatttgaacg gccagataaa agaaaaggaa gcgataaagg ctgaagctga
     1501 aaaggaggca ttggagctta aaagcgacgt ggaaaaatta aacgaagaag aaactctcat
     1561 ggaaataatt gagaaaatag caagggacca atataagatg gttaaaccca atgaaacaat
     1621 atacatagat aaaaacaaaa atgacaataa gcttattcaa gggataggct cgcaaaaaga
     1681 cttgataaat gaataaattt ttgtgattta agcatatttt gacagtattt tacaatcata
     1741 tttgatacca tagcggaaac gaggggtgaa aatatgattg gaagaacaca ggatgaaaaa
     1801 tatgatttaa tcatcactaa cacattaaag aaagaactta aacacatcaa agagaattgg
     1861 ctgctgcttt tggtgtgttt ttttgtttca aggcagaata taatcaatga aatatttccc
     1921 tttgccattg ttgtattaag ttcatactgt cacattaagg gtccctggat ttcaatactg
     1981 tttgtgtgca tagcagcaat tttatcggta cgttttgatt ttgtgtacat cattatgctt
     2041 acggccatat acggatatta ttttaatttc cgtaatgaag gcaaaaaatc aatattatta
     2101 atatccggat attccgctgc agtattgttt ttttcaaaaa cagctatctt ggtttccgaa
     2161 gggttcaata ccaacgaatt catgcttaat gtatttgaaa caatg
//
LOCUS       DAOSOW010000329         2202 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_49558_length_2202_cov_3.363763, whole genome shotgun sequence.
ACCESSION   DAOSOW010000329 DAOSOW010000000
VERSION     DAOSOW010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49558_length_2202_cov_3.363763"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="PLD67_07990"
     CDS             complement(<1..1173)
                     /locus_tag="PLD67_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOT22502.1"
                     /translation="MNFKEKILAFMKEEAYKPLTFKELLQELGIEGKMKKALLVALNE
                     LEDEGKIIFTRSERYGVPEKMNLVTGTLEGHQKGYAFLIPDDKSLKDLFISPVDMNGA
                     MHGDRVIVRPMKVLDDVKSPEGKVIRIIERANQYVIGTFQKSRHFGFVVPDDKRISFD
                     IFVPREEFNNAKENNKVLVKITEWPDQRKNPEGTIVEVIGDIEDTKTHIEAVLLAKKV
                     RQIFPVDVIKEAKKVSEEGIHELELKRRKDLRNLNIITIDGSDAKDLDDAVYAEKLND
                     NEYKLGVHIADVAHYVKEDGKLDKEALKRATSIYISDRVIPMLPKELSNGVCSLNPGE
                     DKLTLSVEMTINKDGKVIDYAIFESIITNRYRMTYDDVTEILENNDEELIEKYKEIV"
     gene            complement(1206..>2202)
                     /locus_tag="PLD67_07995"
     CDS             complement(1206..>2202)
                     /locus_tag="PLD67_07995"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOT22503.1"
                     /translation="HDINNYKKKNFDDAYEVLPGFKNEKLILYPHPELKTGFFGKKYP
                     TYDIDIVFPAVHGTNVEDGALQGLLQINGVPYAFGSVLSSAVGMDKVIMKKVFQSCNL
                     PIVDYIWFYRSSFEADKDKIIEEAENIGYPLIVKPANLGSSVGINKAKNYDELLFAIE
                     VAMSYDRKIIIEKCLENVREINCAVIGYENELETSLCEEPVGWKEFLTYEDKYVNKKK
                     DASESKRRIPAEIEEDIEIKIKDLAKETFKAVDGCGNARIDFLLSGNNIFVNEINTIP
                     GSISFYLWEGKGVSFKQLITRIIELAEIQYKQRKENIVSYDIDLLNKMGGAGKHK"
BASE COUNT          596 a          451 c          293 g          862 t
ORIGIN      
        1 aacaatttct ttgtattttt ctataagttc ttcatcattg ttttcaagaa tttccgtcac
       61 atcgtcatag gtcatcctgt atctgtttgt tattatgctt tcaaatattg cataatcaat
      121 taccttgccg tctttattta tagtcatttc aacggaaaga gtaagcttat cctctccggg
      181 gttcaaactg catactccat tagacagctc tttcggaagc attggaataa ctctatcgga
      241 aatataaata ctggttgctc ttttcaatgc ctctttatct aatttcccgt cttctttgac
      301 ataatgagct acatcggcaa tatggacacc taacttatat tcattgtcat ttagcttttc
      361 ggcatagact gcatcatcca aatcctttgc atcagagccg tcaattgtaa ttatattaag
      421 gttccttaaa tcctttctgc gctttaattc taattcatgt attccttctt cagaaacttt
      481 tttagcttcc tttataacat ccacaggaaa aatctgtctt accttctttg ctaacagtac
      541 cgcctcaata tgtgttttag tatcttcaat atcgccgatt acctcaacta tagtcccttc
      601 tggattcttt ctttggtcag gccactcggt tatttttacc aagactttat tgttttcttt
      661 tgcattgtta aactcttctc ttggaacgaa tatatcaaag gaaattcttt tgtcatcagg
      721 taccacaaaa ccgaaatgcc tgcttttttg aaatgtaccg attacatatt gattagccct
      781 ttcaattatt ctaatgactt tcccttcagg acttttaaca tcgtcaagaa ccttcatagg
      841 tcttacgatt actctatcac cgtgcattgc accattcata tccacaggtg aaataaaaag
      901 gtcctttaaa cttttatcat caggtattaa aaaggcatat cctttttggt gcccttccaa
      961 tgtacctgtt accaagttca ttttttcagg tacgccgtat ctttcagacc tggtaaatat
     1021 tattttacct tcatcttcca gttcatttaa agcgaccaaa agggcttttt tcattttccc
     1081 ttcaattcca agttcttgaa gaagttcttt gaatgttaaa ggtttatatg cttcctcctt
     1141 cataaatgca agtatttttt ctttaaagtt catctttttc tcctgagtgt gattagtcat
     1201 atatattatt tatgttttcc tgcgccaccc attttattta ataaatcaat gtcgtaggaa
     1261 acaatgtttt cttttctttg cttgtattgt atttctgcaa gctcaataat tcttgtgatt
     1321 aattgtttaa atgatactcc ctttccttcc cacagataaa aagatattga gcctggaatc
     1381 gtgttaattt cattaacaaa tatattgttg ccacttagaa gaaaatctat tcttgcattt
     1441 ccgcatccat cgactgcttt gaaagtttcc tttgccaagt cttttatttt tatttctata
     1501 tcttcttcta tttctgcagg tatgcggcgt tttgattcac ttgcatcttt ctttttgttc
     1561 acatatttat cttcataagt taaaaattct ttccagccga caggctcttc gcaaagagaa
     1621 gtttcaagtt cattttcata tccgattacg gcacagttta tttctctaac attttccaaa
     1681 catttttcta taattatttt tctgtcataa gacattgcta cttcaatagc aaataataat
     1741 tcatcataat tttttgcttt gtttatacca acgctggagc ctaaattagc aggctttaca
     1801 atcaaaggat atcctatgtt ttctgcctct tctattattt tatctttatc ggcttcaaaa
     1861 ctgcttctgt aaaaccatat ataatctact ataggaaggt tgcagctttg aaacaccttt
     1921 ttcattatta ccttatccat tccgactgca gagcttaata ccgagccaaa tgcataagga
     1981 acaccgttta tttgcaataa accctgcaga gcaccatctt ccacatttgt gccgtggact
     2041 gcaggaaata caatgtcaat atcatatgta ggatattttt tcccgaaaaa acctgtttta
     2101 agttcaggat gaggatagag tattaatttt tcatttttaa atcccggcaa aacttcatat
     2161 gcatcatcaa agtttttttt cttataattg tttatgtcat gt
//
LOCUS       DAOSOW010000330         2200 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_49620_length_2200_cov_5.381818, whole genome shotgun sequence.
ACCESSION   DAOSOW010000330 DAOSOW010000000
VERSION     DAOSOW010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49620_length_2200_cov_5.381818"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1992
                     /locus_tag="PLD67_08000"
     CDS             100..1992
                     /locus_tag="PLD67_08000"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOT22504.1"
                     /translation="MRKYLGSILVVCFMLLLTEQLKSADYSSLIGKWERQLSAGASFT
                     LEFNSNKTFILKSYTLGIRNTISGTVIIKNDTYICSGDTASFTFKISYVDNARLVIQG
                     LNGTVETWYRSTTTGTTNQEPQSSQSENRNIDVNNEFLQAVNDNDIEGVKKALNSGAN
                     VNAVGEDNWIPLVYASANGYVELVKILLIKGANINAKNNSGWTALIAASDAGHIEIVK
                     TLLLKGADVNMKDKNNNTALMSASFKGHIEIVKMLLLRNAELNVRFKNSYTALMLACS
                     KGYIEIVKLLLLKGADVNLKYGDSYYTAMMSASIEGNLEITKVLLSKGADVNAKDKDG
                     GTALMYACLYGHIEIVKVLLQKNANVNIKSSNGGTALMNACLKGHLEVVKLLVSKGAE
                     VNAKALNGGTALINACLNGFLDVMKLLVSKGADVNSRTDDGFTALMVACFKGYFEIVN
                     ILVLNRADVNAKDRDGNTILMAACLKGHLEIVKTLLLKGADVNAKNTDGWTALMAACL
                     KGHLEVVKFLVIKNANVGAKSLVVITENDVKIPVGSTALKIAELLLHADIVDFLSNPT
                     AIEEYRRQNQSIPQQTQARQPAESDGNSQAAPGTNRMSDNEVRTQGNQSQYQDNTQQG
                     QQPGTN"
BASE COUNT          799 a          286 c          439 g          676 t
ORIGIN      
        1 tcttgctaaa ataatttttt tatgataaaa atataaaaag ttacttcggg tttttagagc
       61 cgccgagttg tatgtaataa aaatcttagg aggattttta tgagaaaata tttaggttca
      121 attcttgttg tttgtttcat gcttttactc actgagcagc taaaatcagc ggattattct
      181 tcattaatag gcaaatggga aagacagcta tctgctggag cgagttttac tttagagttt
      241 aattctaaca aaacttttat acttaaatct tatactcttg gaattaggaa tactatttcg
      301 ggaacagtaa taattaaaaa cgatacttat atttgttcgg gtgatacagc ttcatttact
      361 tttaaaattt cttatgttga taatgcgaga cttgtaatac agggattaaa cggtaccgta
      421 gagacttggt atcgaagtac aacaaccgga actacaaatc aagagcctca atcctcacag
      481 agtgagaata gaaatattga cgtcaacaat gaatttttgc aagctgtcaa tgataatgac
      541 attgaaggtg taaaaaaagc tttaaattca ggggctaatg ttaatgcagt aggagaagat
      601 aattggattc ctttagtgta tgcttctgct aatggatatg ttgagttagt taaaatactt
      661 ttaattaaag gagctaatat aaatgctaaa aataattctg gttggacagc attaatagct
      721 gcaagtgatg ctggacacat tgaaatagta aaaacacttt tattaaaagg tgctgatgtc
      781 aacatgaaag ataagaataa taatactgct ctgatgagcg caagttttaa agggcatatt
      841 gaaatagtaa aaatgttgct attaagaaac gcagaattga atgttagatt taaaaatagt
      901 tatactgctt taatgcttgc gtgttctaag ggatatattg aaatagttaa gcttctttta
      961 ctaaaaggag cggatgttaa tctaaaatat ggagatagtt attatacagc aatgatgagc
     1021 gcaagtattg agggtaatct tgagattaca aaagtgctat tgtcaaaagg agctgatgtt
     1081 aatgctaaag acaaggatgg tgggacagca ttaatgtatg cctgtttata tgggcatatt
     1141 gaaatagtaa aggtgttact acagaaaaat gctaatgtaa atataaaatc atcaaatggt
     1201 ggaacagcat taatgaatgc ttgtcttaaa ggacacttag aagttgtaaa actccttgtt
     1261 tcaaaaggag ctgaagttaa tgctaaagca ttaaatggtg gaacagcatt aataaatgca
     1321 tgtcttaatg gtttccttga tgtgatgaag cttcttgttt caaaaggagc ggatgttaat
     1381 tccaggacag acgacggttt cactgcatta atggttgctt gttttaaagg atattttgaa
     1441 attgttaata tacttgtatt aaatagagct gatgttaatg caaaagatag agatggtaat
     1501 actattctaa tggctgcttg tttgaaaggg catttagaaa tagtgaaaac tcttttattg
     1561 aaaggtgcag atgttaatgc taaaaatact gatggttgga ctgcattaat ggctgcttgt
     1621 cttaaaggac atttagaagt tgtgaaattt cttgtaatta aaaatgcaaa tgttggtgca
     1681 aaaagtcttg ttgttattac tgaaaatgat gttaaaattc ctgtgggttc aactgcattg
     1741 aaaattgctg aactgctttt acatgcagat atagtagatt tcttatcaaa tccgacagca
     1801 attgaagaat atcgacgcca aaatcaatcg ataccacagc aaacgcaagc ccggcaacct
     1861 gctgaatcag atggcaattc acaagctgct ccaggtacca atagaatgag tgataacgaa
     1921 gtaagaaccc agggaaatca atctcaatat caggataata cacaacaagg acaacaaccc
     1981 gggacgaact aaaaattgaa catatcgaaa aaggtttgcc ggaattccga taagcctttt
     2041 tccattatca aagaaagaat attaggggta tttatgacta aaaatgaagg caaaaaacta
     2101 atacaagcga aaattgatga attcaaagag aatgagccaa taattaaaaa gaaaggttat
     2161 ggggaaacaa atacccgtac aaattacatt gatgtgcttt
//
LOCUS       DAOSOW010000331         2188 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_50021_length_2188_cov_2.377872, whole genome shotgun sequence.
ACCESSION   DAOSOW010000331 DAOSOW010000000
VERSION     DAOSOW010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50021_length_2188_cov_2.377872"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            50..1237
                     /locus_tag="PLD67_08005"
     CDS             50..1237
                     /locus_tag="PLD67_08005"
                     /inference="COORDINATES: protein motif:HMM:NF015649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HOT22505.1"
                     /translation="MKFYISTLGEFDIKVDGQSILRDSNRMYRIYKLFEYFLTFRNKK
                     LLPETIIDNVLSDSESEDPKNMLRTQIFRLRKIINALIPKGELPEKYMNLNFTNGYYC
                     LEIGENTIVDIDVFENLIQKGDKEREYDIESAIESYQNAISLYKGLYLSDNAYEVWLV
                     PTRNYYQRLFLKTLNKYIDLLKRNEENEKIVNLCEKSLLIEPFEESIHIELIEALLKI
                     GQNKSAMQHYEYALNMLEKELDAKPSAKFINYINIIQNYTSRNNDLDIEAINKSLEEE
                     LPEGAMYCSLEYFKFLFNLQKRKSKRNNEYDYLLSIMINSNNNKSINDVFSVLKKSLR
                     KGDVFTSWNDNQILVMLHNVKENGTETIKERLTDNLINYTRINRNEIRLIFQPVSMEN
                     TIL"
     gene            1445..>2188
                     /locus_tag="PLD67_08010"
     CDS             1445..>2188
                     /locus_tag="PLD67_08010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013388732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOT22506.1"
                     /translation="MEYPSKYDFYLKFKPVYNKEGYFLGYVLIYVSDFFINDINIGAD
                     LTIGKKFSEIVVDSDILGLKEFYFNIIPKSKMKLELYNEDLKRWYLINAFTDMSNRDE
                     HLIIHYADITEIKQNQQTVSLDNNIYYLNDRERLLYKDKLTGLYNKTYFEEELSRLDT
                     KRQLPISLIMGDINGLKLINDAFGHSMGDSVLKKAAEIMTNSFRDEDIISRIGGDEFV
                     ILLPMTVEKTALKIVERVKRKCENNPLEYI"
BASE COUNT          866 a          283 c          404 g          635 t
ORIGIN      
        1 agggaaaatt aacagggttt attagagggg attattcaag ggggaaatca tgaagtttta
       61 tattagtact ctgggagaat ttgatataaa ggttgacgga caatcaatat tgagagattc
      121 aaatcgcatg tacaggatat acaagctatt tgagtatttt ctaacattca gaaataaaaa
      181 attattacct gaaacaatca ttgacaacgt gctgtccgac agtgaatcgg aagaccctaa
      241 gaacatgctt cgaacacaga tttttcgcct tagaaagata ataaatgctt taattcctaa
      301 aggcgaactt ccggaaaaat atatgaattt aaactttaca aacggatatt actgtcttga
      361 aataggtgaa aataccatag ttgatattga cgtattcgaa aaccttatcc aaaaagggga
      421 caaggaacga gaatatgaca ttgaaagtgc aattgagagt tatcaaaatg caatcagcct
      481 ttataaggga ctgtacttat ccgataacgc atatgaagta tggttggtgc ctacaagaaa
      541 ttactatcaa agattatttt taaaaacatt gaataaatat atagatttat taaaaagaaa
      601 tgaagaaaac gaaaaaattg taaatctatg cgagaaatcg ctattgattg agccgtttga
      661 agaaagtata catattgaat tgattgaagc gcttcttaaa atagggcaga acaaatctgc
      721 gatgcagcat tacgaatatg cattaaatat gcttgaaaag gaattggatg caaagccttc
      781 cgctaagttc ataaactaca taaatataat acaaaactac acttcaagaa acaatgattt
      841 ggatattgaa gcaattaata aaagcttgga ggaagagctt ccagaaggcg caatgtattg
      901 cagtcttgag tattttaaat tcctttttaa tttacaaaag agaaaatcca agaggaataa
      961 tgaatatgat tatcttttat caataatgat taacagcaat aataacaaaa gtataaatga
     1021 tgtattttcc gtattaaaaa aatcgttgag gaaaggtgat gttttcacca gctggaacga
     1081 taatcaaatt ttagtaatgc ttcataatgt gaaagaaaac ggtacagaaa caattaaaga
     1141 aagactgacc gacaatttaa taaattatac aagaattaac agaaatgaaa tcagactgat
     1201 ttttcaacct gtcagcatgg aaaacacgat actgtagatg tgttggaagc aaagcaaaaa
     1261 gtccgttatt gtggactttt tttattgccc ctgataaaaa gtggtgtgtc ccagcacctt
     1321 atgtaattaa attgtaatta aaaagtaatc aatattaaat tgcataataa tagaaatgta
     1381 attcatagag attataatta aaataaatat gaaggaaaaa cgtctaagaa ggcgaggaga
     1441 taaagtggag tacccttcaa aatacgattt ttatttaaaa tttaaaccgg tatacaacaa
     1501 ggaaggctat tttttgggtt atgtcttgat atatgtcagt gattttttta ttaatgatat
     1561 caatatcggg gctgatttga ccataggcaa gaaattttcc gaaatagttg tagattcgga
     1621 tattttaggt cttaaggaat tttattttaa catcataccc aaatctaaaa tgaagcttga
     1681 attatacaat gaagatttaa aaagatggta cctcataaat gcctttacgg atatgagcaa
     1741 cagggatgag catcttataa ttcattatgc ggatataaca gagataaaac aaaaccaaca
     1801 aaccgtatct ttagataata atatatatta tttaaatgac agggaaagac ttttatataa
     1861 ggataagtta accggattat acaacaaaac ctattttgag gaagaactgt caaggcttga
     1921 caccaaaaga cagcttccta taagtctgat aatgggggat ataaacggac tcaaacttat
     1981 caatgatgca tttggtcaca gcatgggtga cagtgtactg aaaaaagcag cggagataat
     2041 gactaattca tttagagatg aagatataat cagcaggata ggcggtgatg aatttgtaat
     2101 tttattgcct atgacagtgg aaaaaactgc tttgaaaatt gtcgagagag taaagagaaa
     2161 atgtgaaaac aatcccttgg aatacatt
//
LOCUS       DAOSOW010000332         2163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_50842_length_2163_cov_2.607685, whole genome shotgun sequence.
ACCESSION   DAOSOW010000332 DAOSOW010000000
VERSION     DAOSOW010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50842_length_2163_cov_2.607685"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PLD67_08015"
     CDS             complement(<1..339)
                     /locus_tag="PLD67_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22507.1"
                     /translation="MKNIKFKTLALILAILVIISGCVSQNNGIDKENNSSIVEDTEDI
                     VGNDTQSPVNNSNEGTGEEDRQEQEDQQDRQEPQEKQTELSIDENCTYTDPYLVAEYI
                     HKYNKLPSNYL"
     gene            640..>2163
                     /locus_tag="PLD67_08020"
     CDS             640..>2163
                     /locus_tag="PLD67_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOT22508.1"
                     /translation="MQRAILSKKNQITIVSGILLINALFGHFILKSEEIVLPSLVVAS
                     IFGVAPIAIQAYQALRVKVVSIDVLVTIAVTGAFLIRNFEESAIVTFLFLFGAYLEQR
                     TLNQTRSAIKELTEMAPESALKQMENGEFEEVEVDEVDVGDILLVKTGAKVPVDGTVL
                     TGEGYINEASITGESVPVGKKKDSQVYAGTILENGTIQIVADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLSFIVWLFSRDIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARHGVLLKGSEVFQDFSRVDTIVFDKTGTLTIGNPKVADKEIYADNVD
                     EVLAYLSSVENESDHPLAKAIVEYIGDTNVYEVQKTDVVKGGGIVAEVDGHRVAVGNV
                     KLMEQENVHINEKALADIERFEKSGNSIVLISVDGELKALIGIRDQVRPGVRENLLKL
                     KKLGVKNLVVLSGDNQGTVDLVANELGLTEAHGHMLPEDKSAFIKNLIDNGHIVAFVG
                     "
BASE COUNT          713 a          308 c          458 g          684 t
ORIGIN      
        1 tagataatta gagggtaatt tattatattt atgaatgtat tctgctacca agtaggggtc
       61 tgtatatgta cagttttcgt ctattgataa ttctgtttgc ttttcttgtg gttcctgacg
      121 gtcttgctgg tcttcttgtt cttggcggtc ttcttcgcct gtaccttcat tgctattatt
      181 tactggcgat tgagtatcat tacctacaat atcctctgta tcttcgacaa ttgacgaatt
      241 attttcttta tctataccat tattttggga cacacatcct gatattatca ctagtattgc
      301 tagaatcaga gctaatgtct taaattttat atttttcatt tgctgtattc tcctactgtt
      361 attaaactga ctaattcctc cggtggtaac gacttccatt gttattatag caaaaattgc
      421 aatagtatta aacttatttt tgccatggaa tgtataaaaa cagggttgaa caaaaattat
      481 aatatttttt taattttgat ttaaatcaat tcatattact aaaataactg ctacaataca
      541 attaaagaat aatataaaaa cgaatgcagg agaaaatatg tcaataaaac tacataaaat
      601 aatagattat aatataaaac caaaaaaagg agggagtgca tgcaaagagc aatattaagt
      661 aagaaaaatc aaataacaat agtaagcggg attttgttaa taaatgcatt atttggtcat
      721 ttcattttaa aaagcgaaga aatagtctta ccgtcattgg tagttgcttc aatctttggg
      781 gttgcaccta ttgcaattca ggcataccaa gcattgagag taaaagttgt aagtatcgat
      841 gtactagtta ccatcgctgt caccggagca tttttaatca gaaactttga ggaatcagcc
      901 attgttacat tcttatttct atttggagca tatttagagc agcgtacatt gaatcaaacg
      961 cgttcagcaa tcaaagaatt aaccgaaatg gcgccggaaa gtgcattgaa gcaaatggag
     1021 aatggtgaat ttgaagaagt ggaagtagat gaagttgatg taggggatat tttacttgtt
     1081 aaaaccggtg caaaggttcc tgttgacggt actgtattaa cgggagaagg atatattaat
     1141 gaagcaagca tcacaggtga atctgttcct gttggtaaaa agaaagactc tcaagtatat
     1201 gcaggaacaa tacttgaaaa tggaaccatt caaattgttg ccgatagagt aggagaggac
     1261 actaccttcg gaaaaataat tgagttggtt gaagaagctc aagattcaaa atcagaagcc
     1321 gagcgtttca ttgacagatt ctctaaatat tacacgccgg cagtcttggt tctatccttt
     1381 attgtatggt tgttttcaag ggatattgaa cttgcaatta caatattggt attgggatgc
     1441 ccgggagcat tggttatagg tgtacctgtt tcaaatgttg caggtatcgg taatggagct
     1501 aggcatggtg ttttgttaaa aggcagtgaa gtttttcagg atttcagcag ggttgataca
     1561 atagttttcg acaaaacggg tacattgaca ataggaaacc ctaaagttgc cgacaaggaa
     1621 atttatgcgg ataatgttga tgaagtatta gcttatctgt caagtgtaga aaatgaatct
     1681 gaccatccat tggctaaggc aattgtagaa tatattggag atactaatgt ctatgaagtt
     1741 caaaaaactg atgttgtaaa aggcggcggc attgtggccg aagtagatgg acacagggtt
     1801 gctgtcggta atgtaaaact gatggaacaa gaaaatgttc atataaacga aaaggctctt
     1861 gctgatatag aaagatttga aaaaagcggt aactcaattg tactaatatc ggttgatggt
     1921 gaattgaagg cactgatagg tattcgtgac caggttcgcc caggtgtaag agaaaattta
     1981 ctaaagctta aaaaattagg tgtcaaaaac ttggtagttc tatcgggaga caaccaggga
     2041 acggttgatt tggtagcaaa tgaattaggt cttacagaag ctcacggaca tatgcttccg
     2101 gaagataagt cggcatttat caagaattta atagataatg ggcatattgt ggcatttgta
     2161 gga
//
LOCUS       DAOSOW010000333         2146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_51374_length_2146_cov_3.575801, whole genome shotgun sequence.
ACCESSION   DAOSOW010000333 DAOSOW010000000
VERSION     DAOSOW010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51374_length_2146_cov_3.575801"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..903)
                     /locus_tag="PLD67_08025"
     CDS             complement(91..903)
                     /locus_tag="PLD67_08025"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOT22509.1"
                     /translation="MKKVDLHIHTNASDGTWDVTELKNELKKNKIDIFSVTDHDSVEN
                     IENMQNILKNKDNLIFIPGTELSAEYKGKEYHLNLYNYDIKNKELLELMKWTNQNKLN
                     SNVDYINYAANKYKNISVEDFERYANDRKRGGWKSANYMIDKGIHKDIPEHLKDVYES
                     GLKAELKSPEEVINIAKKSGGKIFLAHPSYHYRDSVMPIEELKHWGELGIDGIECYSP
                     YNRDNTRYYIDFCKKNNLMISGGSDCHGSFIPSRKLGVPDVSLDDLNIKKLL"
     gene            complement(949..1944)
                     /locus_tag="PLD67_08030"
     CDS             complement(949..1944)
                     /locus_tag="PLD67_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOT22510.1"
                     /translation="MKKILMIATGGTIASKITEHGLAPAISSDELLSYVPEIKKYCYV
                     DTIQLLNIDSTNIQPEHWVMMTETIEENYDKYDGFIVSHGTDTMAYTAAALSYLIQNP
                     CKPIVLTGSQKPINVDITDAKKNLLDSFRFVTEEGISGVYVVFDGKAIIGTRARKVKS
                     KSYSAFESINFPVAAIIDDRRITKYISNYNLEKKVKFYKEIYTNIFLLKLAPGMEPDV
                     LDFIGNKYEGIVIESYGAGGLPFLDKRNFLNKLEELIKKGKIIVVATQVMFEGSDMGL
                     YEVGVKALQKYNVLQAYDMTIEAVITKLMWIMAQTKDFEEVKTMFYTRIHEDSLY"
     gene            complement(1953..>2146)
                     /locus_tag="PLD67_08035"
     CDS             complement(1953..>2146)
                     /locus_tag="PLD67_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019413401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOT22511.1"
                     /translation="IRVHLKHIGHPIVGDTLYGNESLLIDRQALHCYEMKFKHPITNK
                     ELIITCPMPEDMKRVIEGR"
BASE COUNT          583 a          438 c          301 g          824 t
ORIGIN      
        1 tccatccttt ttccttttcc ggctgcaact attattgcag aaatatgttt tttgttttgc
       61 atatcttcct ccaaacgggg cattggctaa tcataataat tttttaatat tcaaatcatc
      121 caaagaaaca tcgggaactc ctaatttcct ggaaggtata aagcttccgt ggcaatcgga
      181 gccgcctgat atcattaaat tgtttttttt gcaaaaatca atataatatc gggtattgtc
      241 cctgttataa ggagaataac actcaattcc gtctattcca agctcccccc agtgttttaa
      301 ttcttctatg ggcataacag aatccctata gtgataggat ggatgtgcaa ggaatatttt
      361 ccctccgctt ttcttcgcta tgtttattac ttcctccggg cttttcaatt cagccttaag
      421 tcccgattca taaacatcct tcaagtgttc ggggatatct ttgtgtattc ctttatcaat
      481 catgtagttg gctgacttcc agccccctct ttttctgtca ttcgcatatc tttcgaaatc
      541 ttcaacacta atatttttat atttatttgc agcataattt atataatcaa catttgagtt
      601 taatttgttt tgatttgtcc acttcattaa ctcaagcagt tctttgtttt ttatatcata
      661 attgtataaa ttcaaatgat attctttccc tttatattca gcggatagtt cggttccggg
      721 aataaaaatt aaattgtcct tattttttaa tatattttgc atattttcga tgttttcaac
      781 actgtcatgg tccgtaacgg aaaatatgtc aattttattt tttttcaatt cattttttaa
      841 ttctgtcaca tcccaagtgc catcagaagc attggtgtgt atgtgcaagt caaccttctt
      901 catatttgtc ttgtttgata tagcaagcag ctaccatata ttcctttctt agtatagaga
      961 atcctcgtgt attctggtat aaaacattgt ttttacttct tcaaaatctt tggtttgagc
     1021 cataatccac atcaatttcg taattacagc ctcaatggtc atgtcataag cttgcagaac
     1081 attatatttt tgaagtgcct ttactcctac ctcatataag cccatgtcgc ttccttcaaa
     1141 cattacctgg gtagcaacaa caattatttt cccttttttt ataagttctt caagtttatt
     1201 taaaaaatta cgtttgtcaa gaaaagggag tcctcccgca ccatagcttt caattactat
     1261 tccttcatat ttattgccta taaaatcaag aacatccggc tccattcccg gagctaattt
     1321 caataaaaat atgtttgtgt aaatttcctt ataaaacttt acttttttct ctaaattata
     1381 attacttata tattttgtaa tacgcctgtc atctattatt gccgcaaccg gaaaattaat
     1441 actttcaaaa gcactgtaac tttttgattt tacttttctc gcccttgttc cgattatcgc
     1501 tttgccgtca aataccacat atacaccgct tatgccctct tccgtaacaa acctgaagct
     1561 gtcaaggaga tttttctttg catcggttat atcaacattt atgggttttt gagagcctgt
     1621 taaaacgatt ggcttgcatg gattttgaat taaataagaa agagctgctg ctgtatatgc
     1681 cattgtatct gtaccgtgag acactataaa accatcatat ttgtcataat tttcctctat
     1741 cgtttcagtc atcatgaccc agtgttccgg ttgtatatta gtgctgtcaa tatttaaaag
     1801 ctgaattgta tccacatagc aatatttctt tatttcaggc acataactta aaagttcatc
     1861 tgagcttata gcaggtgcca gaccgtgttc ggttatcttt gaagcaattg taccccctgt
     1921 cgctatcatt aaaatctttt tcatattccc tcctaccttc cttcaattac ccttttcata
     1981 tcttccggca taggacatgt tattattagc tctttatttg taatcggatg cttaaacttc
     2041 atttcatagc agtgtagtgc ctgccggtct attaataagc tttcatttcc atacaatgta
     2101 tcaccaacaa taggatgacc tatatgcttt aagtgaaccc ttattt
//
LOCUS       DAOSOW010000334         2134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_51772_length_2134_cov_4.634921, whole genome shotgun sequence.
ACCESSION   DAOSOW010000334 DAOSOW010000000
VERSION     DAOSOW010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51772_length_2134_cov_4.634921"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLD67_08040"
     CDS             <1..672
                     /locus_tag="PLD67_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HOT22512.1"
                     /translation="TGTARGLKKAGAQSQIVAASVDLSGLHMASDTQFNRKSFTTGHT
                     GFGMPFSTWPDRSDVPRSAARPLRYIDRYLIVHQGEVFYMTEALASLEGLEKGPAGNT
                     SLTAAFALAQELDQDQTIVVQETEYTGAGKHIQPQLSFARANGIEVKFGDPKDEIPGK
                     NIILPSHPSLIKANDADVIAMRKSLIRNALNNYKVTPTEADIEFLAAETNSSVEFVKE
                     TIASL"
     gene            740..1090
                     /locus_tag="PLD67_08045"
     CDS             740..1090
                     /locus_tag="PLD67_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HOT22513.1"
                     /translation="MKRADDYQQRRAHLANLTDEELYERFWKLANEIVDPLLELGKKN
                     TTPSVERSILLRMGFSSLEVKPIVEGVMNKGLMGKGAGNVVWRLSKKLGCSVREAGLL
                     LAEGKHWDDVDTLF"
     gene            1102..>2134
                     /locus_tag="PLD67_08050"
     CDS             1102..>2134
                     /locus_tag="PLD67_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOT22514.1"
                     /translation="MKLEPNKKLDIKELLKDLDKYEPRRRGWHWREGRNEKRTVGDFE
                     YHETSECLKNSIPLPGSRGFGYIDPQPDCVITTEIASGRFEDDIRRMRMAAWHGADHL
                     MVIRTAGQSHIDSLLEGTNQGIGGIMVTRKQVRASRKACDLIEDEIGRPINFHSYISG
                     VAGPDIAVMFAEEGVNGAHQDPQYNVLYRNVNMVRSFVDACEAKKIMAWADMLQIDGA
                     HNANATAMKAWKVMPELMVQHAINTAFSVRVGIKPENIALSTVPPTAPPAPCMRLDLP
                     YAVALRDLFKDYKMRAQQNTKYMESDTREAAVTHTLNMVISRLTSADIQSTITPDEGR
                     NVPWHYNNIA"
BASE COUNT          752 a          361 c          477 g          544 t
ORIGIN      
        1 acgggaactg caagaggact taaaaaggca ggagctcagt ctcaaatagt ggctgccagc
       61 gtagatttgt cgggacttca tatggcatca gacacacagt ttaacagaaa atcatttacg
      121 acaggacata caggattcgg aatgcctttt tcaacatggc cggaccgctc ggatgttcct
      181 cgctcggcag caagaccatt aagatatatt gacagatatt taatagttca tcaaggggaa
      241 gttttctata tgactgaagc attagcatct cttgaaggat tggaaaaagg tcctgccgga
      301 aatacttcac ttacagctgc atttgcactt gcacaggaat tagaccaaga tcaaacgata
      361 gttgttcagg aaacagaata tacaggcgca ggcaagcata ttcagcctca gttgtcattt
      421 gcaagagcca atggaataga agttaaattt ggagacccaa aggatgaaat tccgggaaag
      481 aatataattt taccttctca tccttcattg attaaagcaa atgacgcaga tgtgatcgct
      541 atgagaaaat cattaataag gaatgcactg aataattaca aagtaacacc tacggaagct
      601 gacatagaat tccttgcggc agaaacaaat tccagcgtgg aattcgtaaa agaaacaatt
      661 gcatctttgt agaatattaa acaaataggc gtatgaaata ttacgcctat ttgaaattta
      721 aaaattacag gaggatttta tgaaaagagc tgatgattac cagcaaagaa gagctcactt
      781 agcgaatctt acagatgaag aattatatga aagattttgg aaattagcaa atgaaattgt
      841 agaccctctc ttggaattag gaaagaaaaa tacaacacca tccgttgaaa gaagtatatt
      901 gcttcgaatg ggattttcaa gtcttgaagt taaacccata gttgaaggtg taatgaataa
      961 gggtttaatg ggaaaaggtg caggcaatgt tgtttggaga ttatcgaaga aattaggctg
     1021 ctcagtgcgt gaagcaggat tattgcttgc agaaggcaaa cactgggatg atgttgatac
     1081 actattttag gagggatgag gatgaaactt gaacctaaca aaaaattaga tataaaagaa
     1141 ttattaaaag atttagataa atatgaaccg agacgccgcg gatggcattg gagggaagga
     1201 agaaacgaaa aaagaacagt aggtgacttt gaatatcatg aaacatcaga gtgtttaaaa
     1261 aattcaattc cccttcccgg aagcagagga tttggatata tagacccaca gcctgactgt
     1321 gtaattacta ctgaaattgc ttcaggaaga tttgaagatg atataagaag aatgcgtatg
     1381 gctgcatggc atggtgcaga ccatcttatg gttataagga cagccggtca gtcacatatt
     1441 gactccctgc ttgaaggtac aaaccaaggt atcggcggta ttatggttac aagaaagcaa
     1501 gtgagagctt caagaaaagc ttgtgattta attgaagatg aaataggtcg tccgataaac
     1561 ttccactcat atatttcggg tgttgcaggt cccgacatag cagttatgtt tgccgaagaa
     1621 ggagttaacg gagcacatca agacccgcaa tacaatgttt tgtacagaaa tgttaacatg
     1681 gtaagaagct ttgttgatgc ttgtgaagct aaaaagataa tggcatgggc agatatgctg
     1741 caaatagacg gagctcacaa tgccaatgct acagcaatga aggcttggaa agttatgcct
     1801 gaattgatgg tacaacatgc tataaataca gccttctcag taagagtggg cataaaacca
     1861 gaaaacatag cattgtctac cgtaccgcca acggcacctc ctgcaccttg tatgagacta
     1921 gacttaccat atgcagtagc tcttcgtgat ttgttcaaag attataaaat gagagctcag
     1981 caaaatacaa aatatatgga atcggatact cgtgaagcag cggtaactca tacattgaac
     2041 atggttatat caaggcttac aagtgcagat attcaatcta ccatcactcc tgatgaagga
     2101 agaaacgtgc cttggcatta taacaacatt gcag
//
LOCUS       DAOSOW010000335         2132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_51830_length_2132_cov_4.037554, whole genome shotgun sequence.
ACCESSION   DAOSOW010000335 DAOSOW010000000
VERSION     DAOSOW010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51830_length_2132_cov_4.037554"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1170)
                     /locus_tag="PLD67_08055"
     CDS             complement(<1..1170)
                     /locus_tag="PLD67_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT22515.1"
                     /translation="MLIIECSKIKKYYGDRLILDIDNLNIYSEDRIGVVGLNGAGKTT
                     LINILSQRIEPDKGWVKLRGKTEYFSQLEEPEVYEISSEMASKFGVETLWNENMSGGE
                     KTRFKLAKALENESILIFADEPTSNMDIEGIELMEKKLTEYKGALIIISHDRSFLDKL
                     CNKIIEIENGKIKTYEGNYSDYSRQRLHDKERAQFEYEEYIKEKERLGSVIADTKQKI
                     KSIKRPPKRMGNSEARLHKMGGQKAKANLERKVGNLEKRIEHLEVKEKPKKISKIKID
                     IMPSDYIHSKIIIEGKKVNKSFGNKIIFKDAEFCIYNGSKVALIGPNGCGKSTLVKMI
                     IDKDNSIRLSKGAKIGYFSQDMNILNNSLNIIDNVMESCNYSENFARLLLARLLFK"
     gene            complement(1468..1974)
                     /gene="lepB"
                     /locus_tag="PLD67_08060"
     CDS             complement(1468..1974)
                     /gene="lepB"
                     /locus_tag="PLD67_08060"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003376628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOT22516.1"
                     /translation="MKTFINEWVIPSIIAVVIVLFLNRFIFILVTVPTGSMETTIMPG
                     DRLYVNKLFDIKDAKRGDILVFQSDELDARLVKRLIGLPGETVEINATGEVFINGEKL
                     DEPYINNANGEERTFKIPEGTYFFLGDNRPVSVDARYWSNPFIPEDKIIGKVAFRFFP
                     FNRIGKVE"
BASE COUNT          619 a          422 c          248 g          843 t
ORIGIN      
        1 tttaaataaa agtcttgcaa gtagaagcct tgcaaaattt tcggaataat tacagctttc
       61 catcacattg tcaataatat ttaaactgtt attcaatatg ttcatatctt ggctaaaata
      121 gcctatcttt gcacctttgg acagcctgat tgaattatcc ttatctatta tcatctttac
      181 caaggtactt tttccgcagc cgttaggacc tatcaatgct acctttgaac cattataaat
      241 acaaaactcg gcatccttga atataatttt attcccgaag gatttgttta ctttcttccc
      301 ttcaattatt attttactgt gaatataatc ggaaggcata atatctattt taattttaga
      361 tattttttta ggtttttctt tcacctctaa atgttcaatt cttttttcca aattgcctac
      421 tttgcgctct aaatttgcct tggctttctg accgcccatt ttatgaagcc tcgcttctga
      481 atttcccatt ctcttaggtg gtctttttat actttttatc ttttgtttag tatcggctat
      541 tacacttcct aatctttcct tctcttttat atattcttca tactcaaatt gtgctctttc
      601 tttatcatga agtctttgtc tgctgtaatc actgtaattg ccttcataag ttttaatctt
      661 tccgttttca atttcaataa ttttattaca tagtttatct aaaaagcttc tatcgtgaga
      721 aattattatc aacgcaccct tgtattccgt tagcttcttt tccataagct caataccttc
      781 aatatccata ttgctggtcg gctcatctgc aaaaattaat atactttcat tttctaaggc
      841 tttagctaat ttgaatcttg ttttttctcc accgctcata ttttcattcc ataatgtttc
      901 cacaccaaat tttgaagcca tttcactgct gatttcataa acttccggct cctctaactg
      961 tgaaaagtat tctgttttac ccctaagctt aacccatcct ttgtcaggct ctatcctttg
     1021 gcttaaaata tttattaatg tagtttttcc tgctccgttt aaacctacca cacctatcct
     1081 gtcttcagag tatatgttta aattatctat atctaatatt aatctgtctc cataatattt
     1141 ttttatttta ctgcactcaa taataagcat aaaaaatcct ccctttcttt ctttggagga
     1201 tagcattttt actttacaca atatatctaa ggatatgcat aataaatctt gcatagtaat
     1261 aataaagtaa cagcagctat taaaatacta tccacctgta atacaataaa atgcacaata
     1321 aaaaatcttt aatttaattg tcattataat tgtttttcaa gcggattatt tcattctcta
     1381 taccttacct ttcttaattt ctttacatta caaaaaatta tatatactgt caagattctt
     1441 ttattctgca tgttgtaaat agttctatta ttcgacctta cctatccggt taaaggggaa
     1501 aaatctgaaa gcgaccttcc cgataatttt atcctccggt ataaaaggat tgctccagta
     1561 ccttgcatct acagatactg gtctgttatc gcccaaaaaa aagtatgtgc cttcagggat
     1621 tttgaatgtt ctttcttctc cgtttgcatt attgatatac ggctcatcga gcttttcgcc
     1681 atttatgaac acttcacctg ttgcatttat ttcaacagtt tctcccggca atccaataag
     1741 tctttttaca agccttgcat ccagctcatc tgattggaat acaagaatat ctcccctttt
     1801 tgcatccttt atgtcaaaaa gtttgttaac gtaaagtctg tcaccgggca taattgttgt
     1861 ttccatggag ccggttggaa ccgtcaccag aataaatata aatctgttta aaaaaagtac
     1921 aattacaaca gcaataattg aaggaataac ccattcattt ataaatgtct tcattttcac
     1981 gtccttaatc ttatttatac tgaacttatt atattacctg ataaccatta taacaattta
     2041 tttcttatca agcaataaaa attcataact taaaaagatt gtaagaaatt aggattatag
     2101 attgattatg cttttaatta tcttaattgc ta
//
LOCUS       DAOSOW010000336         2128 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_51967_length_2128_cov_3.973951, whole genome shotgun sequence.
ACCESSION   DAOSOW010000336 DAOSOW010000000
VERSION     DAOSOW010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51967_length_2128_cov_3.973951"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLD67_08065"
     CDS             complement(<1..555)
                     /locus_tag="PLD67_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOT22517.1"
                     /translation="MFQFEEPSERLANIKVIGVGGGGNNAVNRMIDAGVRGVNFISIN
                     TDKQALGLSKAETKFQIGEKLTNGLGAGSKPEIGEKAAEENKQDIAELVEGADMVFIT
                     AGMGGGTGTGAAPIIANIAKSMGILTVAVVTKPFMFEGRCRMRNAEMGLKRLKDHIDT
                     LVVIPNDKLLEITDKKTTMLEAFAM"
     gene            complement(691..1035)
                     /locus_tag="PLD67_08070"
     CDS             complement(691..1035)
                     /locus_tag="PLD67_08070"
                     /inference="COORDINATES: protein motif:HMM:NF018632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1290 domain-containing protein"
                     /protein_id="HOT22518.1"
                     /translation="MIYVAIGLILGIVAGLNLNFIYNPDYIVYISLAILAILNTIINI
                     LHENKTSELTITKSIAFLLTDLLFALFLGFVGEQLGLPIYLAAVFAFGNNIYKKIKVI
                     VDIYLEKYFINK"
     gene            complement(1032..1763)
                     /locus_tag="PLD67_08075"
     CDS             complement(1032..1763)
                     /locus_tag="PLD67_08075"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOT22519.1"
                     /translation="MSTIKKTLNIIIFVLVFLTAAAIVNGADAGIDEYTIYTIRDLNK
                     YAHSIKEMKLLVNNMRNAVESEKRTIADYESKKYLDDIIEIKSQIEQIKLYNGFTAVR
                     GPGIMLRVSDNINEDYDLELMERIVHDVDIVVLLNDLKAAGAEAIEVNGKRIINISEV
                     VCAGPLIRVNGEVIPAPFVIIAIGDMDELYDAVTEEGTYAYELKKTYGMEVVVAQGYN
                     LTVREFRGINYETKYAEIVEEGEKK"
     gene            complement(1767..>2128)
                     /locus_tag="PLD67_08080"
     CDS             complement(1767..>2128)
                     /locus_tag="PLD67_08080"
                     /inference="COORDINATES: protein motif:HMM:NF017734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HOT22520.1"
                     /translation="LSLASSHQYILALISYLNNAGVEAISINGQRYTNYTEIVPVLEH
                     INVNGVAMVLPLEIKAIGDRSTINSSLNFVGGIVSQLNEIGYIIETEDSDEIFIPRYD
                     GEKQFKYALPIIRDEEI"
BASE COUNT          607 a          444 c          287 g          790 t
ORIGIN      
        1 catggcgaaa gcttccagca tggttgtttt cttgtctgtt atctccaaaa gtttatcgtt
       61 cggaataaca accagagtat ctatatgatc ctttaacctt ttaagtccca tttcagcatt
      121 tctcattctg catcttcctt caaacataaa aggtttagtt acaactgcaa ccgtaagtat
      181 acccatagat ttggcaatat tagcaataat aggagctgca ccggttccgg ttcctccgcc
      241 cataccggcg gttatgaaaa ccatatctgc gccttctacc agttctgcta tatcttgttt
      301 attttcttca gctgcttttt ctcctatttc cggttttgag ccggctccta atccattagt
      361 taatttttca cctatttgaa attttgtttc tgcttttgat aagccaagag cctgcttgtc
      421 cgtatttatg ctgataaaat tcacacctct gacaccggca tctatcattc tgtttactgc
      481 attattgccg cccccgccta cacctattac tttaatattg gcaagtcttt ctgatggttc
      541 ctcaaattga aacatatttt gacctcctga gagagaaatt tttcatacta tttaaactat
      601 tctatcataa atttaataat attcatacat attttttata atctacacca attttccgaa
      661 tattttttca gaattatcac aatattgagt ttacttgttt ataaaatact tttcaagata
      721 tatatcgact atgactttta tctttttata aatattatta ccgaatgcaa atactgcagc
      781 taaataaata ggcaaaccta attgctcgcc cacaaatccc aggaacaaag caaataacaa
      841 gtctgttaac aagaatgcaa tactttttgt aattgtcaat tctgatgttt tattttcatg
      901 aagtatattt ataattgtat ttaatattgc cagtattgcc agtgatatat acactatata
      961 atccggattg taaataaaat ttaagttcaa tcctgcaact atacctaaga ttaatccgat
     1021 tgcaacatat atcatttctt ttccccttct tctactattt cggcatattt ggtttcataa
     1081 tttattccgc gaaattctct gaccgttaaa ttatatcctt gtgcaacaac tacttccatt
     1141 ccgtacgttt tcttcaactc gtaagcataa gttccttctt cggtaactgc atcatacaat
     1201 tcatccatat ctccgattgc tattatgaca aaaggagcag gtattacttc accgtttact
     1261 ctgattaaag gtcccgcaca tactacttca gatatgttaa ttattctttt cccgttaact
     1321 tcaattgctt ccgctcctgc tgcctttaaa tcgtttaaca gtacaacaat atctacatca
     1381 tggactattc tttccattaa ctcaaggtcg tagtcttcat tgatgttgtc tgaaactctc
     1441 agcattatac ccggtcctcg cacagccgta aacccattat atagtttaat ctgctcaatt
     1501 tgagatttaa tttcaattat gtcgtctaaa tatttttttg attcatagtc ggcaattgtt
     1561 cttttttcgc tttctacagc attgcgcata ttatttacca agagcttcat ttctttgata
     1621 ctgtgtgcat atttgttcaa atctctgatt gtatatatgg tatactcatc tattcctgca
     1681 tctgcaccgt ttacaattgc tgcagctgtc aaaaaaacca gcacgaaaat tattatattt
     1741 aatgtttttt taatagtgct cactccttag atttcttcat cccgaattat tggaagcgca
     1801 tacttgaact gtttttctcc gtcataacgc ggaataaaaa tttcatccga atcttccgtt
     1861 tcaattatat atcctatttc attaagttga gaaactatac ctcctacaaa gttaagagaa
     1921 gaattgattg tagatctgtc tccgattgcc tttatttcca aaggaagtac catggcgaca
     1981 ccgtttacgt taatatgttc aagaaccggc actatttccg tatagttggt atatctttgt
     2041 ccattgatgg aaatagcttc aacaccggca ttgtttaaat agctgattaa agctaatata
     2101 tactgatgac tggatgcaag acttaaat
//
LOCUS       DAOSOW010000337         2114 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_52449_length_2114_cov_3.098106, whole genome shotgun sequence.
ACCESSION   DAOSOW010000337 DAOSOW010000000
VERSION     DAOSOW010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52449_length_2114_cov_3.098106"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1148
                     /gene="hrcA"
                     /locus_tag="PLD67_08085"
     CDS             90..1148
                     /gene="hrcA"
                     /locus_tag="PLD67_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOT22521.1"
                     /translation="MSLDKRKVKILKAIISSYIDNAEAVGSRTISKKYELGVSPATIR
                     NEMSDLEDMGYLVQPHTSSGRIPTEKAYRYYVDDLWKKVKTSKNPKLDQLREIIEDKS
                     NELDEIFKNSVRILSQFTKYTSFVVAPQFKQSVIKRIHLVPVSEYKVLMLLILETNIV
                     KQVMLRLSSPIPFNQLEKISESLSEKMHGYRLEDIDKIKDNLIKELYYLRESNNDILM
                     DLLPYLVEQIAKFEDVNVYSDGITNILELPEYNDVVKAREFISFFEDKTSVARLMQYY
                     GENDLDIYIGSENKSQELRDCSLITATYKFNGKLIGRIGVIGPTRMDYNNVISTVKTM
                     SEAINEIIRLNFNGENKE"
     gene            1153..1758
                     /gene="grpE"
                     /locus_tag="PLD67_08090"
     CDS             1153..1758
                     /gene="grpE"
                     /locus_tag="PLD67_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOT22522.1"
                     /translation="MAKGKKHDEVNENVKDLEETEETMAESKEDSQRSDKTSETKEEK
                     VEEKPAEETENNELKKKNEEIEALNNKLLRLQADYLNYKDRVEKEKLTIYGDAVSAVI
                     CDLLPVIDNLERAISADVPDNFKEGVVMVYNQLMGILNKKGLKEIEALHKPFDHNLHY
                     GVAYEESEEFDDGIVIDVLQKGYTVNDKLIRPSMVRICKKS"
     gene            1869..>2114
                     /locus_tag="PLD67_08095"
     CDS             1869..>2114
                     /locus_tag="PLD67_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOT22523.1"
                     /translation="MGKIIGIDLGTTNSCVSVMEGGEAVVIANVEGKRTTPSIVAFTK
                     DGERLVGETAKRQAVTNPDRTITSIKRDMGSNRKINID"
BASE COUNT          857 a          302 c          427 g          528 t
ORIGIN      
        1 attgacaaag ttcatcgtac gtagtatctt aatattatgg agttagcact caaccaagga
       61 gagtgctaac aaagaaaagg agtgagaaaa tgtctttgga caagagaaag gtaaagattc
      121 taaaagctat tatctccagc tacattgata atgctgaagc tgtaggctca aggacaattt
      181 caaaaaaata tgaacttgga gtaagccctg ccaccatcag aaatgaaatg tctgatttgg
      241 aagacatggg ttatttagtt cagccacata cttcatcagg acgaataccg acagaaaaag
      301 catacagata ctatgtggat gatttatgga aaaaagtcaa gacttcaaaa aatcctaaat
      361 tagaccagct tagagaaata attgaggaca aatcaaacga attagatgaa atattcaaaa
      421 attcagttcg aatactgtcc caatttacta aatatacttc atttgtagtt gcaccacagt
      481 ttaagcaatc tgttattaaa agaatccatt tagtaccggt ttcggaatat aaggtattga
      541 tgctcttaat actggagacc aatattgtca aacaggtaat gctgagatta agcagcccca
      601 taccttttaa ccaacttgaa aaaatatccg aatcactttc cgaaaaaatg cacggctata
      661 gattagaaga tattgacaaa attaaggata acttaattaa agagctatat tatttgagag
      721 aatcaaacaa tgatatattg atggatttgc tgccctattt agtggagcag atagcaaagt
      781 ttgaagatgt taatgtatat tcggacggta taacaaatat attggagctt cccgaatata
      841 atgatgttgt taaagcaaga gaatttatat cattctttga agataagacc agtgttgcca
      901 gattgatgca atattacggc gagaatgatt tagatatata tataggcagt gagaataaaa
      961 gccaggaatt acgggattgc agcctcatta ctgcaaccta taaatttaac ggcaagctaa
     1021 taggaagaat cggagtaata ggaccgacaa gaatggatta taacaatgta atttcaactg
     1081 ttaaaacaat gtcggaagca ataaatgaaa taattagact aaactttaac ggagaaaaca
     1141 aggagtgaag aaatggcaaa aggtaagaag catgatgaag ttaatgaaaa tgtaaaagat
     1201 ttggaagaga cggaagagac tatggcagaa tctaaagaag attcacaaag gtcagataaa
     1261 acttctgaaa caaaagaaga aaaggttgaa gaaaaaccgg ctgaagaaac agaaaataat
     1321 gaattaaaaa agaaaaatga agaaatagaa gcacttaaca ataaattgtt aaggcttcaa
     1381 gcagactact taaactataa agacagggta gaaaaagaaa agctcacaat atacggggat
     1441 gcggtatcag cagtgatttg cgatttgctt cccgtaattg acaaccttga aagagcaatt
     1501 tcagcagatg tgccggataa ttttaaggaa ggcgtagtga tggtttataa tcagcttatg
     1561 ggaattttaa ataaaaaggg gttaaaagaa attgaagccc tgcacaaacc ttttgaccat
     1621 aatctgcact acggagtggc atatgaagaa agtgaagaat tcgatgacgg aatagtaatt
     1681 gatgttcttc aaaaaggata tactgtaaat gataaactca taagaccgtc aatggtcaga
     1741 atatgtaaaa agtcgtaatc ccttgtcatt ctgaacgaag tgaagaatct catgaagtcc
     1801 ttcgttaaac tcaggatgac aataaaatat agaaaatatt aaattcaaat cataaatgga
     1861 ggaataaaat gggcaaaatt ataggaatcg acttaggtac aacaaactcg tgcgtatctg
     1921 taatggaagg cggagaagcc gttgttatag ctaatgtgga aggcaaaaga accacacctt
     1981 caatcgtggc attcacaaaa gatggagaaa gattagtcgg agaaacagcc aaaaggcagg
     2041 cagtcacaaa ccccgataga acaataacat caataaaaag agacatgggc tctaacagaa
     2101 aaattaatat tgat
//
LOCUS       DAOSOW010000338         2109 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_52585_length_2109_cov_4.177215, whole genome shotgun sequence.
ACCESSION   DAOSOW010000338 DAOSOW010000000
VERSION     DAOSOW010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52585_length_2109_cov_4.177215"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /gene="dut"
                     /locus_tag="PLD67_08100"
     CDS             complement(<1..289)
                     /gene="dut"
                     /locus_tag="PLD67_08100"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004824123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOT22524.1"
                     /translation="MKIKVVKKNPFKLPEYETKGSAGVDLQAYVDAPLILKPMERALV
                     PTGLFIELPEGYEAQVRARSGLAIKHGISLVNGIGTIDSDYRGEIKVILINL"
     gene            complement(279..1559)
                     /locus_tag="PLD67_08105"
     CDS             complement(279..1559)
                     /locus_tag="PLD67_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOT22525.1"
                     /translation="MIKKHTLDNGLRIVMEKIDYVKSASIGIWVNVGSYNENEETNGL
                     SHFIEHMLFKGTKNRKANEIAEEIDNLGAQMNAYTSKECTSFYVKVLEENINEALDIL
                     SDMFFNSLFSPEEIEKEISVVIEEIKMYEDSPEDIVYDKLSEIIFNDSPMAFNILGTE
                     KNLKTFTSDKVKKYMEENYSPSNTVISIAGSFDEDEFIKLIEEKFKHWENKDVKRLNK
                     NSDYQRKIRGINKDLEQLHICIGNKTFGRHDKNYHPLLVLNNLLGGTASSRIFQEVRE
                     KKGLVYSIYSFVTNYSDTGIFSIYAAMSYEQVEEALRTILKEMASIKNGNIKEAEFNR
                     AKQQIKGNFILSLESTSSRMSAIGRRELLYNEILYPDEIIESINNVKIDDVLDVAKEL
                     FNIDNLSITFAGNLHKHSDIEDKIYKVLGEEYEN"
     gene            complement(1619..1801)
                     /locus_tag="PLD67_08110"
     CDS             complement(1619..1801)
                     /locus_tag="PLD67_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22526.1"
                     /translation="MKFFCNVAFIRKLLIIIFLVIIIILLIIGRFRTMRVKMVGKEVW
                     GNTLMHCERDTHHNDG"
BASE COUNT          604 a          408 c          284 g          813 t
ORIGIN      
        1 cgagattaat taaaattact tttatttctc ctctgtaatc cgagtcgata gttcctatac
       61 cgtttacaag gcttataccg tgtttgattg ccaaaccgct tcgagctctc acttgtgctt
      121 cgtaaccttc gggaagttca ataaataacc ctgtgggcac caaggctctt tccatcggct
      181 ttaatattaa gggtgcatca acataggcct ggaggtctac tcccgcagag ccttttgttt
      241 catattcagg taatttaaat ggatttttct ttactacttt aattttcata ttcttctccc
      301 agtactttat aaattttatc ttcaatatca gaatgtttat gtaaattccc cgcaaatgta
      361 atactcaggt tgtcaatatt gaacaattct tttgctacat ccaaaacatc atcaatttta
      421 acattgttaa tgctttcaat tatttcatca ggatacagga tttcattata taagagctct
      481 cttctgccga tagctgacat tctgctcgat gtgctttcaa gactcaatat aaaatttcct
      541 ttaatttgct gctttgctct gttaaattct gcttctttaa tatttccgtt tttgatgctt
      601 gccatttcct ttagaattgt tcttaaagct tcttcgacct gctcgtagct catggctgca
      661 taaatgctga aaatacctgt atcggaataa ttggttacaa atgaataaat agaatatact
      721 agaccttttt tctctcttac ttcctgaaaa attcttgagc tcgccgttcc ccccaacaaa
      781 ttgttcaata ctaagagagg atgataattt ttgtcatgcc ttccaaatgt tttattgcct
      841 atgcatatgt ggagttgttc caaatcctta ttaatacccc ttatttttct ttggtaatct
      901 gagtttttat taagacgttt tacatcttta ttttcccaat gtttaaattt ttcctcaatc
      961 agcttaatga attcatcctc atcaaaactt ccggcaattg aaattacagt attagacgga
     1021 gaataatttt cttccatata tttttttact ttatcactag taaaagtttt taaatttttt
     1081 tcggtaccta gaatattgaa tgccataggg ctgtcgttaa aaattatttc agataattta
     1141 tcgtatacaa tatcctcggg cgaatcttca tacattttaa tttcttcaat aacaactgat
     1201 atttcttttt ctatttcctc aggtgaaaac aaagagttaa aaaacatatc cgatagtata
     1261 tccaatgctt cgtttatatt ttcttccaaa acttttacgt aaaagctggt gcattccttg
     1321 cttgtgtatg cattcatctg agcgcctaaa ttgtcaattt cctctgcaat ttcatttgct
     1381 tttctgtttt ttgtgccctt gaaaagcatg tgctcaataa agtgagacaa tccgtttgtt
     1441 tcttcatttt cattataaga accgacatta acccatatac cgatggatgc agactttacg
     1501 tagtcgattt tttccattac aatccttaag ccgttatcta atgtgtgttt ttttatcatt
     1561 tttcctccgc ttactacatg ctaatgctta tatttcttca tggactcact tcatagtctt
     1621 acccgtcatt atggtgcgta tctctttcac aatgcattag ggtgtttccc catacctctt
     1681 tacctaccat tttaactctc atagtcctga atctgcctat tataagtaaa attattataa
     1741 ttaccagaaa aattataatc aataattttc gtatgaaagc aacattgcaa aaaaatttca
     1801 taaaaatccc cctctatact taaatgtatt attcatgagg gatatttatg attattattt
     1861 gtatgtgctt ccttccttta tcctaccaaa tatttgcagg aaattcaagt ttttaaatat
     1921 tgacagacta aattgacgac caaaaggata agctgttcac aggctccacg tccaccatat
     1981 aaattacact tcgtaagacc tgccgctgtt ttattagggt aaaggaaaac agcgtagggt
     2041 ctttaaaaaa taacaaatac ataataatat gatatttgtt atcttaagtg ttttattatt
     2101 tttgtactt
//
LOCUS       DAOSOW010000339         2094 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_53099_length_2094_cov_3.284453, whole genome shotgun sequence.
ACCESSION   DAOSOW010000339 DAOSOW010000000
VERSION     DAOSOW010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53099_length_2094_cov_3.284453"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..1168
                     /locus_tag="PLD67_08115"
     CDS             119..1168
                     /locus_tag="PLD67_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HOT22527.1"
                     /translation="MNKKDEPESDSKFKSFLRRKNIEISAQRYLIEALGLMAYGLFAS
                     LLIGTILNTIGKKFGISFLTETVWPICSQMTGAAIGVAVAYGLKAPPLVLFASTITGT
                     AGNILGGPAGAFCSALVGAEFGKLVSKETKIDIIITPAVTIIAGVLIASLVGPAIGAF
                     MSATGEFIMYATTLRPFLMGILVSVVVGIVLTLPISSAALCMMLGLSGIAAGAATAGC
                     CAHMVGFAFLSFKENGWGGVAAQGLGTSMLQMPNLVRNPRCWIPAVIVSAITGPMATV
                     LFHMENSPIGAGMGTSGLVGIIETLNTMGGGMKVWLGILILYILIPVVLTPLIGNILR
                     KVGWIKDGDLKLDLV"
     gene            1268..1906
                     /locus_tag="PLD67_08120"
     CDS             1268..1906
                     /locus_tag="PLD67_08120"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOT22528.1"
                     /translation="MAQLYFRYSTMGAGKSIEVQKVAFNYEERGQNPLIFTSSIDNRF
                     AESGIVASRIGLSRRAIPVYDDMNIFEVIKEYNDIFGVDAVIIDECQFMKKHHIQELT
                     DVVDELNIPVMCYGLRADFRNELFEGSYWLFALADKIEEIKTICWCGKKALTNARIVD
                     GKVVYVGEQIFIGGNESYMPLCRKHYKEGKIMPYREKGEDLIIRYEKLKKEK"
     gene            1946..>2094
                     /locus_tag="PLD67_08125"
     CDS             1946..>2094
                     /locus_tag="PLD67_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HOT22529.1"
                     /translation="MAIFEDFMKLDIRVGEIVEAKNFEKARKPAYKLIVDFGHEIGTK
                     KSSAQ"
BASE COUNT          684 a          299 c          494 g          617 t
ORIGIN      
        1 gaagagaaat gtaaaaagaa ttccggggac ggacctcaaa attccggtaa tactgggatg
       61 aagtcaccgg aaggtccgtt cccgtaatta tggaataaaa ataacaagag ggatgagtat
      121 gaataaaaaa gacgaaccgg aaagcgattc caaatttaaa agttttctaa gaagaaaaaa
      181 tattgaaatt tctgctcaaa gatacctgat tgaagcattg ggtttaatgg cttacggtct
      241 ttttgcatct ttgctgatag gtacaattct caatactatc ggcaaaaagt ttggcatttc
      301 ttttttaacc gagacagtat ggcctatttg cagtcagatg acgggagctg caataggtgt
      361 tgccgtagct tatggactaa aggctccgcc tttagtcttg tttgcatcta caattacagg
      421 aacggcaggt aatatcttag gtgggcctgc aggtgctttt tgcagtgctt tggtgggtgc
      481 tgaatttggt aaattagttt caaaagaaac aaaaatagat attattatta ctccggctgt
      541 tactattatt gcaggtgtct taattgcttc attggtagga cctgctatag gagcatttat
      601 gagtgcaacg ggtgaattta taatgtatgc tacaaccctg aggccttttt taatgggtat
      661 attggtttca gtagtggtag gcatagtatt aacactgcct ataagcagtg cagctctttg
      721 tatgatgctt ggtctttcag gtatagctgc cggcgcagca actgcaggat gctgtgcaca
      781 tatggtaggt tttgctttcc taagttttaa ggaaaacggc tggggaggag ttgcagctca
      841 aggattggga acatcaatgc tgcagatgcc gaatcttgtg aggaatccca ggtgctggat
      901 tcctgccgta atagtatctg ccattacggg tcccatggca acggttttat tccatatgga
      961 aaacagtccc ataggcgccg gtatgggaac cagcggcttg gtaggaataa ttgaaacatt
     1021 aaataccatg ggcggaggga tgaaggtgtg gttgggaata ctaatactat acattttaat
     1081 tccggtggta ctcactccgc ttatcggtaa tattttaaga aaggtcggtt ggattaaaga
     1141 tggcgatttg aagttagatt tagtctagcc taatgtatga cccttaatcc gtatattagg
     1201 tttacataat tgattttatg taaaccttag tgataaaaga aagaaaaaaa caatatagag
     1261 gagagttatg gcacagcttt atttcagata tagcaccatg ggtgctggaa agagtataga
     1321 agtacaaaag gttgcattta attatgaaga aagaggacaa aaccccttaa tatttacatc
     1381 ttcaattgat aacagatttg cagaatcagg aattgttgct tcgagaatcg ggctgagcag
     1441 gagagcaata cctgtttatg atgatatgaa tatttttgaa gtcattaaag agtataatga
     1501 tatattcggt gttgatgccg taataattga cgaatgtcaa tttatgaaaa aacaccatat
     1561 acaagagctt acggatgtag tagatgaatt aaatatacct gttatgtgct atggattgag
     1621 agctgatttc agaaatgaat tattcgaagg ctcttactgg ctttttgcat tagctgataa
     1681 aattgaagaa attaagacta tttgttggtg cggcaaaaaa gcattaacta atgcccgtat
     1741 tgtggacggt aaagtagtat atgtgggcga acaaattttt ataggaggca atgaaagtta
     1801 catgcctctt tgcaggaagc actataagga aggaaagatt atgccttaca gggaaaaagg
     1861 cgaggattta ataataagat atgaaaagct taaaaaagaa aaatagatat gattactcaa
     1921 tagtgattat ataaggagat atcatatggc aatatttgaa gattttatga agttagatat
     1981 cagggtggga gaaatagtag aagcaaaaaa ctttgaaaag gcaaggaagc ctgcttataa
     2041 actgattgtt gattttggac atgaaatcgg aactaaaaaa tcaagcgccc agat
//
LOCUS       DAOSOW010000340         2084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_53435_length_2084_cov_3.827501, whole genome shotgun sequence.
ACCESSION   DAOSOW010000340 DAOSOW010000000
VERSION     DAOSOW010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53435_length_2084_cov_3.827501"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1943
                     /locus_tag="PLD67_08130"
     CDS             <1..1943
                     /locus_tag="PLD67_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOT22530.1"
                     /translation="VKVRRTVGKESKLVESTVGRFIFNEGIPQDLGFVDREKDKFSLE
                     IDKKVTKKILEEIIYRCFRKYGNARTAEVLDGIKQKGFRYSTLGAITISVSDITVPEE
                     KKTLLEQAEKQVLEYEKKYRRGLMTDEERYENVIKIWNQTTEDVTNALMNNLSEINPI
                     YIMSVSGARGGRNQIKQLAGMRGLMASAIGKTVEVPVKSNFREGLSVLEFFLSATGAR
                     KGLTDTALRTADSGYLTRRLVDVSQDVIVREADCGTSDGIEVEDFIDGKEEIERLSER
                     CADRFAADDVVNTETGEIFVKAGEFISEEMAIKIQNSGIKKVKVRSVLTCKTIHGVCA
                     KCYGTNLATGKLVNVGAAVGIIAAQSIGEPGTQLTMRTFHTGGIAAAADITQGLPRIE
                     ELFEARKPKGQAIISEIPGKVTLSERAHKREVTITNYEDKESKTYLIPYGSKLLVNEG
                     DYIEAGDKITEGSINPHDILNIKGMKALESYILMEVQRVYRMQGVDISDKHVEVIIRQ
                     MVNKIRIEESGDTSLLPGSLVSNYEFYQQNEAAIKEGKEPAKGEIALLGITKSSLATD
                     SFLASASFQETTRVLTDAATKGKIDNLIGLKENVIIGKLIPAGTGMKRYRNIGITVND
                     IEKPDDNTEKTDDDEKSIKDAG"
BASE COUNT          758 a          321 c          489 g          516 t
ORIGIN      
        1 aggtgaaggt tagaaggact gtcggcaaag aatccaagtt ggttgagtct acagtgggta
       61 ggtttatatt taatgaagga attcctcagg atttaggatt tgtagataga gaaaaagata
      121 aattttcact agaaattgat aaaaaagtta ccaaaaaaat attggaagaa attatctacc
      181 gctgcttcag aaaatatgga aatgccagaa cggctgaagt tttggacgga attaaacaaa
      241 aaggtttcag atactctacc ttgggggcta ttaccatttc agtaagcgat attacggttc
      301 ccgaagaaaa gaaaactctt cttgaacaag ccgaaaaaca ggttctggaa tatgaaaaga
      361 agtatagaag aggcttgatg acagatgaag aaagatatga aaatgttata aaaatctgga
      421 atcagacaac agaagacgta acgaatgcat tgatgaataa tttaagcgaa attaacccga
      481 tttatattat gtctgtgtcg ggagctcgtg gaggacgtaa ccagataaaa cagcttgcag
      541 gtatgcgtgg tcttatggca agtgccatag gaaaaacagt tgaggttcct gttaaatcaa
      601 acttccgtga aggtctttcg gttctggaat tcttcctgtc ggctacagga gctcgtaaag
      661 gtttaacgga tactgctctt agaacggctg actcgggata cttgacaaga agacttgttg
      721 acgtaagcca ggatgtcata gtaagggagg cagactgcgg aacttcggat ggcattgagg
      781 ttgaagactt tatagacggc aaggaagaaa ttgaaagatt aagtgaaaga tgtgccgaca
      841 ggtttgctgc agatgatgta gtaaatactg aaacaggtga aatatttgtt aaagcaggcg
      901 agtttatttc tgaagagatg gctataaaaa ttcaaaattc cggaattaaa aaagtcaagg
      961 taagaagtgt tctcacttgt aaaacaatcc acggagtatg tgcaaaatgt tacggtacca
     1021 atcttgccac cggaaagctc gtcaatgtgg gagcagcagt gggcattatt gctgcacagt
     1081 caatcggtga accgggtaca cagcttacaa tgcgtacttt ccatacggga ggtattgcgg
     1141 cagctgcaga tatcactcaa ggtcttcctc gtatcgaaga attatttgaa gcgagaaaac
     1201 cgaaaggtca agctataatt tcagaaattc ccggaaaggt aacattgtcg gaaagagcgc
     1261 acaaaagaga agttacgatt accaattacg aagataaaga aagcaagaca tacttaatac
     1321 catacggctc aaaacttctg gtaaacgaag gcgattatat agaagcaggt gacaaaataa
     1381 ccgaagggtc aatcaatcct catgatattt tgaacattaa aggtatgaag gcattggaat
     1441 catacatttt aatggaagtt caaagagttt acagaatgca gggcgttgac atcagcgaca
     1501 aacacgttga agttataatc aggcagatgg tgaacaaaat aagaattgaa gaatccggag
     1561 atacatcact gctgccggga agcttagttt caaattatga attttatcag caaaatgaag
     1621 cagccataaa agaaggtaaa gagcctgcaa aaggtgagat tgcacttttg ggtataacca
     1681 agtcatctct tgcaacagat tcattcttgg cttcggcatc attccaggag accacaaggg
     1741 ttcttacgga tgcagcaaca aaaggaaaga ttgataatct tatcggactt aaagaaaatg
     1801 tcatcatcgg taaactgata cccgcaggta cagggatgaa aagatacaga aatataggca
     1861 ttactgttaa tgatattgaa aaaccggatg ataatactga aaaaacagat gatgatgaaa
     1921 aatcaattaa agatgccggt taaaagcatc attatatgag gaagtacttc aaaagtgctt
     1981 aaattgcaaa aaatatttga catctaaaaa acaagatgat agaatacttg agttgtaagg
     2041 tgtggggaca cacctctaaa gaagggatag tctctgaggg tgag
//
LOCUS       DAOSOW010000341         2077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_53711_length_2077_cov_1.868942, whole genome shotgun sequence.
ACCESSION   DAOSOW010000341 DAOSOW010000000
VERSION     DAOSOW010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53711_length_2077_cov_1.868942"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2077
                     /locus_tag="PLD67_08135"
     CDS             <1..>2077
                     /locus_tag="PLD67_08135"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOT22531.1"
                     /translation="IRQADVRLSIETKLITDNNEDRVNIRVVSVDRKENKINELFIKQ
                     GRRNLSEREIIVIDQFALARGIDIGDEINLNINGRKYRFIVSAIASSPEYVYLMENEQ
                     VLLPDYENFGVAYIEEEYLRKISGSGYFNEVVIRVNNQDNIDKTKDYLEDELKRYGVR
                     RVIDRDEQLSNSMMNEEINGLEKVSKSIPIVFLLFAGIMLAVMLSRIVKKDRTSIGVL
                     KAVGFTDSEIISHYLKYAASVGVIGGFLGSIIGTLASGAMTNMYLEFFNIPMLTVKIY
                     YYRIFISVILSLLFCSASGFWGIRNIIKINPAESMMPEPPKQGKRILLENFKFFWNRL
                     TFTWRIVCRNIFREKKKFIFIAAAVCITCSLMIMTMWMNEIMDIMFVSHYSEFAKMEY
                     NVGFRGFQDERVLKEIKENISYNHMEGRIELPFEIKNGRLSKIVNVIGLEENTVFYDF
                     RDLNGNKLTLHKEGILISSNLAKSLKAEKGDRILLESFIPDTDGEYVTVKGIIEQSLG
                     INGYININYVNRKFLDKGIINGVYINSDDNVKAKLVDINNIMSIQSQEEMQGVFEEFT
                     GLIVMFMGVMVVFSGMLGFIILYSMTLMSINERTLEFSSLRVMGFTKAEIFKMLFKEN
                     MVMSALGIIAGIPLGKWLVDYIGFTFNTDIYTLQGIVTAKDIITAIILTIIFVISAQL
                     MTYAKIHKL"
BASE COUNT          741 a          242 c          420 g          674 t
ORIGIN      
        1 atatcaggca agcagatgta agactgtcta ttgaaaccaa gctgattact gataataatg
       61 aggatagagt taatataagg gttgtatctg ttgacagaaa agaaaataaa ataaatgaat
      121 tgtttattaa acagggaagg cgaaatcttt ctgaaagaga aataattgtt attgaccagt
      181 ttgcccttgc aagaggaatc gatattggtg atgaaataaa tcttaacata aacggcagaa
      241 aatatagatt catagtatcc gctattgctt caagtccgga atatgtttac ttgatggaaa
      301 acgaacaggt gcttcttcct gattatgaaa atttcggagt agcctatatt gaggaagaat
      361 atttgagaaa aatatcagga agcggatatt ttaacgaagt ggttataagg gtaaataatc
      421 aggacaatat tgataaaaca aaggattatt tggaagatga attaaaaagg tacggtgtaa
      481 ggcgggttat agatagagat gagcaattaa gcaacagtat gatgaatgaa gaaattaacg
      541 gtcttgaaaa agtatctaag tccataccaa ttgtattttt actttttgcg ggaataatgc
      601 ttgccgtcat gctgtctcgt atcgtaaaga aggacagaac ttctatcgga gtattaaaag
      661 ctgttggttt tacggatagt gaaataattt ctcattattt gaaatatgct gcttctgtgg
      721 gagtaatcgg aggtttttta ggttcaataa taggaacttt ggcctcggga gcaatgacta
      781 atatgtattt ggaatttttc aatattccca tgcttactgt taaaatttat tactatagaa
      841 tattcatttc agttatatta tcattattgt tctgttcagc atcaggtttt tggggtatta
      901 gaaacataat taaaattaat cctgccgagt ctatgatgcc tgagcctcca aagcaaggca
      961 aaagaatact tttagaaaat tttaaatttt tttggaacag gcttacattt acttggagaa
     1021 tagtttgcag aaatatattc agagaaaaga aaaaatttat atttatagct gcagcagttt
     1081 gcatcacttg cagcttgatg attatgacaa tgtggatgaa tgaaattatg gatattatgt
     1141 tcgtaagtca ttattcagaa tttgcaaaaa tggaatacaa tgtaggattt agaggatttc
     1201 aggatgaaag agttcttaag gaaatcaaag aaaatataag ttacaatcat atggagggaa
     1261 gaattgagct accttttgaa attaaaaatg gcaggctctc taaaattgta aatgtaatag
     1321 gtcttgaaga aaatactgta ttttatgatt ttcgtgattt gaacggaaac aaacttactc
     1381 ttcataaaga ggggatattg atttcttcga atttggcaaa gtctcttaaa gcagaaaagg
     1441 gtgacagaat attattagaa agcttcatac ctgacaccga tggagaatat gtaacagtaa
     1501 agggtataat agagcagagt ctaggaatta atggttacat aaatataaat tatgtaaacc
     1561 gaaagtttct ggataaaggt attatcaatg gtgtatatat taattcagat gataatgtta
     1621 aagctaaatt ggttgacata aataatatta tgtcaattca atcacaggag gaaatgcagg
     1681 gcgttttcga agaattcact ggactcatag tgatgtttat gggagttatg gttgtttttt
     1741 cagggatgct cggatttatt atattataca gcatgactct tatgagtata aatgaaagaa
     1801 ctttggaatt ttcatccctg agggtaatgg gttttacaaa agctgaaata tttaaaatgc
     1861 tttttaagga aaacatggtt atgtcagcac ttggtataat agcaggcata cccttaggca
     1921 agtggctggt ggattatata ggatttactt ttaataccga tatctatact ttacaaggga
     1981 ttgttacagc taaggatata attactgcaa ttattttaac cataatattt gttatttcag
     2041 cacaattaat gacttatgca aaaattcata aacttga
//
LOCUS       DAOSOW010000342         2072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_53886_length_2072_cov_3.764502, whole genome shotgun sequence.
ACCESSION   DAOSOW010000342 DAOSOW010000000
VERSION     DAOSOW010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53886_length_2072_cov_3.764502"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLD67_08140"
     CDS             complement(<1..389)
                     /locus_tag="PLD67_08140"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOT22532.1"
                     /translation="MMENKINTKVVKVDPENIDCNIIKEAADIIKRGGLVVFPTETVY
                     GIGADALNDEAVDKIFKAKGRPQDNPLIVHIAEFHELYDLALEVPEKAELLAEKFWPG
                     PLTMILYKKKILSDKITAGLNTAAIRLP"
     gene            592..1329
                     /locus_tag="PLD67_08145"
     CDS             592..1329
                     /locus_tag="PLD67_08145"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOT22533.1"
                     /translation="MNILDYLIMGFIFGGIGTSIGGLISVLIFKPSEKIISGILSFAA
                     GIMLAVTSFDLMPQAYEIGGFFIVTLGLVIGLIIVFYANDLIPLNKLKQYKGKKLSYI
                     KMSLIIIISISLHNFPEGVAVGSSYVYSDNLGVKIGLLIAAHDIPEGISVGVPLIMAG
                     LSPVMVVFLTLLTGIPTALGAVFGALVGGVSDYFIAFCLSAASSSMLYVSANELIPEA
                     NLLYNGKSSSVLLLLGFMCGCYISFAV"
     gene            complement(1346..1936)
                     /gene="yfbR"
                     /locus_tag="PLD67_08150"
     CDS             complement(1346..1936)
                     /gene="yfbR"
                     /locus_tag="PLD67_08150"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HOT22534.1"
                     /translation="MEKSNFFAFISRMKYIDRWGLMRNINKENVSEHSLDVAVIAHAL
                     AVIQKNRLNMDVNPEKTALYAIYHDVSEIFTGDMPTPVKYYNSVIKSAYKDVELSVNE
                     RLLNMLPEDFKDEFKDILIPKEEEKDIWRTVKAADKISAYIKCLEEEKSGNKEFLKAK
                     QSLLSTIRNMNMPEVKIFMDEFLEGYSLTLDEMEMS"
BASE COUNT          583 a          394 c          350 g          745 t
ORIGIN      
        1 ggcaaccgta tggcagctgt atttaaccct gcagtaattt tatccgataa tattttcttt
       61 ttatataaaa tcatcgttag tggtcccggc caaaattttt ctgccaaaag ctctgccttc
      121 tccggcacct ccaaagctaa atcatacaat tcatgaaatt ctgcaatatg tacaattaaa
      181 ggattgtcct gaggacgtcc ctttgccttg aatattttat cgactgcttc gtcattcaat
      241 gcatcagcac cgattccgta gactgtttcc gtaggaaaaa caacaagacc tcccctttta
      301 ataatatctg cagcttcttt aataatatta caatctatat tttcagggtc aacctttact
      361 acctttgtat ttattttatt ttccatcata taccttctct tgcttggcga tttgccgtca
      421 tcccatgaag gaatgttctc gtaatattat atactacaat aactttatgt acaagaattt
      481 tactgattaa taatttttta atttcttaaa agagtttagg cgcatatact gtatgagggg
      541 ggacacacct tttaaatgtc cctgtactta aagggaacac ggggggttat tgtgaatata
      601 ttggattatt tgataatggg atttattttc ggcggaatcg gaacttcaat cggaggattg
      661 atttccgtac ttatatttaa accaagcgaa aaaataatat ctggaattct aagctttgca
      721 gcaggaatta tgttagcggt tacatccttt gatctcatgc ctcaagcata tgaaatcgga
      781 gggttcttta tagttacctt aggtcttgta ataggtctta taatagtttt ctatgcaaat
      841 gatcttattc ccttgaataa attaaaacaa tataaaggaa aaaagctaag ttatataaaa
      901 atgagcttaa ttataataat cagtataagc ttgcataatt tccccgaagg tgttgccgta
      961 ggctcaagct atgtgtattc ggataatttg ggagttaaga taggattact catcgcagct
     1021 cacgatatac ctgaaggtat aagtgttggt gttcctttga ttatggcagg gttgtctcct
     1081 gtaatggtag tttttttgac tcttcttaca ggtattccaa ctgctctggg agccgtattc
     1141 ggtgcattgg tgggaggtgt atctgattat ttcatagctt tttgtctttc cgctgcatca
     1201 agcagcatgc tttatgtgtc ggcaaatgaa ttaattcctg aagcaaattt actttataac
     1261 ggaaaaagca gctctgtatt gctgcttcta ggtttcatgt gcggatgtta tatatccttt
     1321 gcggtatgat tatttaaaaa tagggttagg acatctccat ttcatccaag gtaaggctgt
     1381 aaccttccag gaactcatcc ataaatattt ttacttcagg catattcata tttcttattg
     1441 tcgaaagcaa tgattgcttt gcttttaaaa attccttatt gccggacttt tcttcttcta
     1501 aacacttaat atatgcactt attttatcgg cagcctttac ggtcctccat atatcttttt
     1561 cttcttcctt aggaatcaag atatccttaa actcatcttt aaaatcttcc ggaagcatat
     1621 ttaaaagcct ttcatttact gacaactcca catctttata ggcagactta ataacagaat
     1681 tgtaatattt aacaggtgtg ggcatatctc ccgtaaatat ttccgataca tcgtgataaa
     1741 ttgcatagag ggcagtcttt tccggattta cgtccatgtt caaccggttt ttttgaatta
     1801 cagccaatgc atgagcaatt acagcaacgt caagactgtg ctcagataca ttttccttat
     1861 ttatatttct cattaagccc caacggtcaa tatacttcat tcttgatata aatgcaaaaa
     1921 aattagactt ttccataaac tctcccctag gtacggacac ctttatatgg tgccaggcac
     1981 ctgttgctgg tgcctggcac cctcttcctc tcttatttaa ttattaactc attaaccttc
     2041 ttatttgtat tgatgctgtg aatttgcatt tt
//
LOCUS       DAOSOW010000343         2054 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_54523_length_2054_cov_3.058029, whole genome shotgun sequence.
ACCESSION   DAOSOW010000343 DAOSOW010000000
VERSION     DAOSOW010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54523_length_2054_cov_3.058029"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLD67_08155"
     CDS             complement(<1..438)
                     /locus_tag="PLD67_08155"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOT22535.1"
                     /translation="MSNIIVLALGGNALGNDPQEQKEAVKHTSKSIVDLIEAGNQVIL
                     SHGNGPQVGMINLAMDAASKSEAGTPEMPFPECGAMSQGYIGYHLQNAIQNELKLRGI
                     KKSVATVVTQVVVDKNDPAFQNPTKPVGSFYTKEEAELMTAKGF"
     gene            608..1867
                     /gene="aroA"
                     /locus_tag="PLD67_08160"
     CDS             608..1867
                     /gene="aroA"
                     /locus_tag="PLD67_08160"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOT22536.1"
                     /translation="MLSKNYKKKSLLIKPSLLKGKITIPPSKSISHRAIICASLSMEE
                     SKISNIVFSDDIRATIEGMKTLGADIKEADDFILVKRSNKILNNAIDCNESGSTLRFL
                     IPLSLVLKDECTFKGKGRLNERPLDIYYEIFRKSNIDYETNAGKLPLKIRGRLKGGFY
                     KIPGNVSSQFITGLFFSLPLLDVGSEIKIIGEPESKNYIKLTLDVMKRYNVNIEKIDE
                     NNYYIKGAQKYKAFDYSIEGDFSQAAFFLAANVLGCKIECLGLNYDSLQGDKEILNIA
                     EKYSSPMEEITIDASQIPDLVPVISVLASLKENCTTKIINAGRLRFKESDRLEATASE
                     MKKLGALIEETKNGLIINGKSSLYGNATVDSHNDHRIAMALAIAAIKCDNPIVLEGYE
                     SVKKSYPKFWDDYQSLGGAIYELNDWK"
     gene            1845..>2054
                     /locus_tag="PLD67_08165"
     CDS             1845..>2054
                     /locus_tag="PLD67_08165"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOT22537.1"
                     /translation="MSSMIGNKLKISIFGESHGEAIGVVIDGLPSGYAINLDYINQQM
                     NRRLPGKSNLSTERKEEDEYKILSGF"
BASE COUNT          713 a          344 c          379 g          618 t
ORIGIN      
        1 aaatccttta gctgtcatta actctgcttc ttccttagtg taaaaagagc ctaccggttt
       61 tgtaggattt tgaaatgcag ggtcgttttt atcaaccaca acttgagtta ctactgttgc
      121 aacagatttt tttatccccc ttagtttcaa ttcgttttgt atagcatttt gtaaatgata
      181 tccgatgtaa ccttgactca tggcaccgca ttccggaaac ggcatttccg gagtgcctgc
      241 ttccgatttt gaagctgcat ccatagccaa atttatcatt ccgacttgcg gaccgttccc
      301 atgagataaa attacctgat ttcctgcttc aattaaatca actattgatt ttgaagtgtg
      361 tttaactgct tccttttgct cctgcgggtc attacccaat gcatttccgc ctaatgccaa
      421 tactataatg ttgctcataa ttcctccttg tatataacaa gggacaagga ttgtccctat
      481 ttacgtaaat ttattgtacc atcatatttt caatttagca atttactttt atctgctatt
      541 caaaaaacta ttgacatacc atgatattgt gataaatttt aatcattacc gtatagcagg
      601 tgttattatg ttaagcaaga actacaaaaa aaagagtttg ttgataaaac cttcattatt
      661 aaaagggaaa attactatcc caccgtcaaa aagtataagc cacagagcaa taatatgtgc
      721 ttctctctcc atggaagaaa gcaaaatcag caatatagtt ttttcggatg atataagagc
      781 aacaattgaa ggaatgaaaa cattgggtgc ggatattaag gaagcggatg atttcattct
      841 cgttaaaagg agcaataaaa ttttgaataa tgcaattgac tgcaatgaat caggctcaac
      901 tctgaggttc cttatacctt tgtccttggt tttaaaagat gaatgcactt ttaagggaaa
      961 gggacgtttg aatgaaaggc ccttggatat atattatgaa atttttagga aatcaaatat
     1021 tgactatgaa acgaatgcag gaaagctccc tctcaaaata agaggcagac taaaaggcgg
     1081 tttttacaaa atcccgggca atgtaagttc tcaatttatc accggattat ttttttccct
     1141 tcctcttctt gatgttggtt cagaaattaa aatcatcggt gaacccgaat caaagaatta
     1201 tatcaagctg acactggatg tcatgaaaag atacaatgta aatattgaaa aaatagatga
     1261 gaataattat tatataaaag gggctcagaa atataaagca tttgactaca gtattgaagg
     1321 tgatttttca caagcagcat tttttcttgc tgcaaatgta ttgggatgca aaattgaatg
     1381 tctaggttta aactatgatt cactgcaggg tgacaaagaa atattaaata tagcagaaaa
     1441 atattcttcg cccatggagg aaataacgat agatgcttct caaataccgg acttagttcc
     1501 cgtaatatcg gttcttgcat ccttgaaaga aaactgcaca accaaaatta taaatgcagg
     1561 aagactgaga tttaaagaat cagacaggct tgaggcaact gcctcagaaa tgaaaaagct
     1621 aggagcttta atagaagaaa caaaaaatgg acttataatt aatggaaaaa gctccttgta
     1681 cggaaatgca accgtagaca gccataatga ccatagaatt gccatggcat tggcaattgc
     1741 agcaattaaa tgcgataatc caattgtttt ggaaggatat gaatcagtca aaaaatcata
     1801 tccaaaattt tgggatgatt atcaaagctt aggaggtgct atttatgagc tcaatgattg
     1861 gaaataaact aaaaataagt atattcgggg aatcacacgg agaagcaata ggtgttgtaa
     1921 ttgacggact gccatcagga tatgcaataa atttggatta cataaatcaa cagatgaaca
     1981 gacggcttcc cggaaaaagt aatttgtcaa ccgagagaaa agaagaggat gaatataaaa
     2041 tactcagcgg tttc
//
LOCUS       DAOSOW010000344         2049 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_54696_length_2049_cov_3.737713, whole genome shotgun sequence.
ACCESSION   DAOSOW010000344 DAOSOW010000000
VERSION     DAOSOW010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54696_length_2049_cov_3.737713"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /gene="rnc"
                     /locus_tag="PLD67_08170"
     CDS             complement(<1..404)
                     /gene="rnc"
                     /locus_tag="PLD67_08170"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOT22538.1"
                     /translation="MITGTVYEFEKIIGYKFNNIKILEESLTHSSYSNEDKAYNKVNN
                     ERLEFLGDAVLSISVSRYIFDEFPDYPEGDLTKLRAQVVCEDTLSLVAANLNLGKYLL
                     LGKGEESSGGRERKSILADAVEAVIAAIYLDGG"
     gene            complement(499..735)
                     /gene="acpP"
                     /locus_tag="PLD67_08175"
     CDS             complement(499..735)
                     /gene="acpP"
                     /locus_tag="PLD67_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010093481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOT22539.1"
                     /translation="MNDVFERIRELVAEKVGVEPEDISMETSFTDDLEADSITLFELV
                     MAIEDEFDIEVDDESIEKIETVGDIVRYLEDIII"
     gene            complement(732..1730)
                     /gene="plsX"
                     /locus_tag="PLD67_08180"
     CDS             complement(732..1730)
                     /gene="plsX"
                     /locus_tag="PLD67_08180"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOT22540.1"
                     /translation="MKIAIDAMGGDNAPEAVIIGSIQARDEYNLSLVLCGKEIEIKRI
                     LEKNTSNFNNIEIINAEDIISNDDKPVLAIRRKKESSMVKALYAVKEGNADAIVSAGS
                     TGALLSGGTLLIGRIKGIERPAIGSLIPNMSGGFCLLIDSGANVDSKPEFLYDFAIMG
                     DIYLKRVMNIKSPRIALANIGVEKSKGNKLTVETYELLVNSQLKLNFIGNIEARDILK
                     GEADIVICDGFTGNMILKTMEGTVLEIMKGLKEEIMSSMKTKLGGMLIKPALKNFKRK
                     FDYSEHGGAPLLGVKAPVIKAHGSSDAKAIKNAIRQAKICGESNVNELIEESIMRR"
     gene            complement(1848..2027)
                     /gene="rpmF"
                     /locus_tag="PLD67_08185"
     CDS             complement(1848..2027)
                     /gene="rpmF"
                     /locus_tag="PLD67_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOT22541.1"
                     /translation="MAVPKRKTSKAKRDSRRAANAKLTVPNTVECPQCHEPKLPHRVC
                     ISCGYYDGKEVVAVE"
BASE COUNT          543 a          464 c          284 g          758 t
ORIGIN      
        1 cctccgtcta aatatattgc agcaataaca gcttccacag catctgccag tattgatttt
       61 ctttccctgc ctccggagga ttcttcacct ttccctaata gtaagtattt ccctaagttt
      121 aaatttgccg caaccaagct tagggtatct tcgcaaacaa cctgggctct caattttgtt
      181 aagtctcctt caggataatc aggaaattca tcaaaaatat atctgcttac ggaaatgctt
      241 aagactgcat ctcccaaaaa ttcaagtctt tcattattta ccttgttata tgccttatcc
      301 tcatttgaat atgagctgtg tgtcaaggat tcttccaata tttttatgtt attaaattta
      361 taacctatta ttttttcaaa ttcataaact gtacctgtaa tcaattttcc ctccggaata
      421 ataacatatc attgctactg gatgacataa ctgatgtctg ctttcaaact atgggacgca
      481 ttgctgcgtc ccccttcttt aaataattat atcttccaag tatctgacaa tatctcctac
      541 tgtctctatt ttttcaatac tttcatcatc aacttcaatg tcgaattcgt cttcaattgc
      601 cataaccaat tcaaaaagtg ttattgaatc ggcttctaaa tcgtctgtaa aggatgtttc
      661 catagaaatg tcttcaggct caactccaac cttttcggct actaattccc taattctttc
      721 aaacacatca ttcacctcct cataatgctt tcttcaatta attcatttac attgctttct
      781 ccgcaaattt ttgcctgacg aattgcattt ttaattgctt ttgcatcaga gctgccgtgt
      841 gcttttatta cgggagcttt aactcccaac agcggagccc cgccatgctc tgaataatca
      901 aattttcttt tgaagttctt aagtgccggt ttaattaaca taccgcctag tttagttttc
      961 atgcttgaca taatttcttc tttcaagccc ttcataattt ccagcaccgt accctccata
     1021 gtttttaata tcatattccc cgtaaacccg tcacatatta caatatcagc ttctccttta
     1081 agtatatccc tggcttcaat gttgccgatg aaattaagct taagctgtga atttacaagc
     1141 agttcatatg tttcaacagt cagtttgttt cccttggatt tttcaactcc tatatttgct
     1201 aaggcaatcc ttggggattt gatattcata actcttttaa ggtaaatatc tcccataatg
     1261 gcaaagtcat ataaaaattc cggttttgaa tccacgtttg caccggagtc tatcaataag
     1321 caaaacccac ctgacatatt gggtatcaat gagcctatcg cgggtctttc aattccttta
     1381 atccttccta tcagaagggt cccgccgctt aataaggctc ctgtgcttcc tgcagatact
     1441 attgcatcag cgttgccttc cttcaccgca tataaagcct tgaccatcga agattctttc
     1501 ttgcgcctta ttgctaagac aggtttgtca tcatttgata ttatatcctc tgcattaatg
     1561 atttctatat tattgaagtt tgatgtgttt ttttctagta ttctttttat ttcaatttct
     1621 tttccgcata gtaccaagct tagattatac tcatctctag cttgtatact tccgattata
     1681 acagcctcag gtgcattgtc tccacccatg gcatcaattg caattttcat attcacctct
     1741 tatctaatat acttatatca gtatatagca gtaatatttg ttttgcaata aaaaaatagt
     1801 gccaagcact atttttttaa atacccgaca cgggttttag agtcttatta ttcaactgct
     1861 accacttctt taccgtcata atatccgcat gatatacata ctctgtgagg tagctttggc
     1921 tcatgacatt gtggacattc cacagtatta ggaacagtta attttgcatt ggctgctctt
     1981 cttgaatctc ttttggcttt ggaagttttt ctcttaggta ctgccatttt tacacctcct
     2041 tatatcaac
//
LOCUS       DAOSOW010000345         2048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_54727_length_2048_cov_4.196187, whole genome shotgun sequence.
ACCESSION   DAOSOW010000345 DAOSOW010000000
VERSION     DAOSOW010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54727_length_2048_cov_4.196187"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PLD67_08190"
     CDS             complement(<1..629)
                     /locus_tag="PLD67_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22542.1"
                     /translation="MSNSYVAVYLITNMFATYTLYRYMCIFFDRKDVDKRQEFLSYSL
                     YFCINSFLYLTFNKPALNIASNLIMFFLLTYNYQSTTKTRLIAVVLIYMILMTIEVLV
                     MLILQNYGITVTSGNDAALITGLISIKIISYIFMLFMSNFKMINNEIKVSNIHWLSIF
                     IIPVGTLILVLIMISMDYASNVTGIIISIMILLVINVFVFYFYDELMKS"
     gene            complement(619..1347)
                     /locus_tag="PLD67_08195"
     CDS             complement(619..1347)
                     /locus_tag="PLD67_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOT22543.1"
                     /translation="MLRIAICDDDKELCIELKSMLNEISKNTGEAFEISVFCNGEGLC
                     DSLMSGNRFNLIFLDIELCEINGVEVGKKIREELNDEITQIVYISAKESYAMELFDIR
                     PLNFLVKPLKIEKIKYVLKTARKVLGSSNQYFEYRVGNVNFNVLISDILYFESSGRKV
                     KVVLKNEVNEYYGKLSEVEEKIKNDDFFFIHKSYLINYNHVIEYSYEYVKMSNNEILG
                     ISQNNRKAVREKLLRKKQRMRHVK"
     gene            complement(1531..1902)
                     /locus_tag="PLD67_08200"
     CDS             complement(1531..1902)
                     /locus_tag="PLD67_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOT22544.1"
                     /translation="MKFLWTTILVNNMDESLKFYEEIVGLKMTERFQAGPGMEISFLG
                     EGETKVELIYNEKFKKLDAGNRVTLGFKVESLDEKINFIKEKGIKIITGPIEPNPSIK
                     YFIIKDPDGVKIQFAQQLQSN"
BASE COUNT          656 a          383 c          248 g          761 t
ORIGIN      
        1 tatgacttca taagttcatc ataaaagtaa aaaacaaaca cattaataac cagcaaaatc
       61 attatgctta ttattattcc ggtaacattg gaagcataat ccatagaaat cataatcagc
      121 actaatatca aggtacccac cggtataata aaaatggaca gccagtgaat attggacact
      181 ttgatttcgt tatttatcat tttaaaattt gacataaaca acataaaaat atatgaaata
      241 atttttatac tgataagccc tgtaattaaa gccgcatcat ttccggatgt tacggttata
      301 ccgtaatttt gaagtatcag catcacaaga acttcaatag tcattaaaat catatatatt
      361 aacacaacag ctatcaacct ggtttttgtt gttgactgat aattatatgt aagcaaaaaa
      421 aacattatta aatttgaagc aatgttgagg gcaggtttat taaaggtcag gtacaagaaa
      481 ctgtttatgc aaaaatataa tgaataagac aaaaattcct gccttttgtc aacatccttc
      541 ctgtcgaaaa atatgcacat atatctgtaa agagtatatg ttgcaaacat atttgttatc
      601 aggtaaacag caacatagct atttgacatg gcgcatcctc tgcttttttc gcagcaattt
      661 ttcacgaact gcctttctgt tgttttgact tattccaagt atttcattat tggacatttt
      721 cacatattca taggaatatt caataacatg attgtaattt attagataag atttatgaat
      781 gaaaaaaaaa tcgtcatttt taatcttttc ctcaacttca gaaagttttc cgtaatattc
      841 gtttacttca ttcttcaata caacctttac ttttctgccg ctgctttcaa aatacaatat
      901 atcacttatt aatacattaa aatttacatt gcctactcta tactcaaaat attgattgct
      961 gctgcctaat actttccttg ctgttttaag aacgtattta attttttcga tctttaacgg
     1021 tttaacaaga aaatttaagg gtcgtatatc gaacagctcc atagcatagc tttccttggc
     1081 tgatatatag actatttgag tgatttcgtc atttaattct tccctgattt tctttcctac
     1141 ttcaacaccg ttaatttcac acaattctat atcaagaaaa atcaagttga agcgatttcc
     1201 gctcatcaat gaatcgcaca gtccttctcc gttacaaaaa accgaaattt caaaggcttc
     1261 gcccgtattt ttgctgattt cattcagcat gctttttaac tcaatgcata gttccttatc
     1321 atcatcgcat attgctattc ttaacataat atccccactc gtaaccttgc ttttgcctag
     1381 aattattaca tgttaattag atatcttttc ttgtatttaa ttgagccgta cttgctgatt
     1441 ttaaaccttt ttattattta aaatcttctc ttcttttttt tagtttgtct aaatcctttt
     1501 ttattaagct tatcaaaatg aacaccttgc ttaattactt tggagctgct gggcaaattg
     1561 aatttttact ccatcagggt cttttattat gaaatatttg attgatggat ttggttctat
     1621 gggacctgtt attatcttta ttcccttttc cttaataaag ttaatctttt catccaatga
     1681 ttcaacttta aaacccaatg ttaccctatt gcctgcatcc aactttttaa atttctcatt
     1741 gtatataagc tcgaccttag tttctccttc acccaaaaaa gatatttcca tcccgggtcc
     1801 ggcctggaat ctctcagtca ttttaagacc tactatttct tcataaaact ttaatgattc
     1861 atccatatta tttacaagta ttgtagtcca caaaaatttc ataattcctc caatttgata
     1921 ttagctaatt tattttacca taataattat actatttcat taaaaattga ttataattta
     1981 acgtgataac agtaaccacc tgacgtattg tctccaaatg tccggcaaga accgtcccct
     2041 atggacca
//
LOCUS       DAOSOW010000346         2046 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_54798_length_2046_cov_4.402310, whole genome shotgun sequence.
ACCESSION   DAOSOW010000346 DAOSOW010000000
VERSION     DAOSOW010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54798_length_2046_cov_4.402310"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="PLD67_08205"
     CDS             complement(<1..201)
                     /locus_tag="PLD67_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOT22545.1"
                     /translation="MYRIQQIKLPIDHKEEELLIKAAEALRIKKEEIKEISIFRKSID
                     ARKKDEILFIYTLDVDTYKEVKI"
     gene            complement(262..678)
                     /locus_tag="PLD67_08210"
     CDS             complement(262..678)
                     /locus_tag="PLD67_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013237097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOT22546.1"
                     /translation="MDQTDLKIISILQNNGRISMKELGKIVSLSPPAVAERVKKLEET
                     DVIQRYTAVINNEKIGKPITVLINASIKPENQKDFLHFAENKEEIAKCHHVTGPHSMI
                     IKANLREMKHLEELVREIQVYGNTETYIIMSSPIDR"
     gene            924..>2046
                     /locus_tag="PLD67_08215"
     CDS             924..>2046
                     /locus_tag="PLD67_08215"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009007629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOT22547.1"
                     /translation="MTKLKITETSLRDGHQSLIATRLSTPEMLPILQEMDKAGYHAME
                     VWGGATFDTCLRYLNEDPWERLRKIRANIKNTKLQMLSRGQNLLGYKNYPDDIVERFH
                     KNAISEGIDIVRVFDALNDIRNLQKTIDTVKSEGAHLQCAISYTISPVHTKEYYLKLI
                     KDFENAGADSVCIKDMSGILLPYTAYDLVKSIKEVTNLPIELHTHCTGGIGEMTVLKA
                     VEAGVDIVDTAISPLSSGTSQPATESLCLTLKDTERDPQLDLMKLNEIADHFKNVMKK
                     FEEEGTLNTKVLMTEPKTLLYQIPGGMLSNLLIQMKSLNAVDKFQAVLDEVPKVREDL
                     GFPPLVTPMSQIVGAQAVFNIVTGERYKVIPTEIRNYVRG"
BASE COUNT          687 a          352 c          343 g          664 t
ORIGIN      
        1 aatcttcact tccttgtatg tatcaacatc taatgtataa ataaaaagta tttcatcttt
       61 tttcctcgca tcaattgatt ttctgaaaat agaaatctcc ttgatttctt ctttcttaat
      121 tcttaaggct tctgctgctt ttattaacag ctcttcctcc ttgtgatcta taggcagctt
      181 gatttgctga attctataca tatcgtacac ctctttaact tcctgtcatt taaaattctg
      241 ccatgtacta tatcaatatc cttatcgatc tatcgggctt gacataataa tatatgtttc
      301 cgtattgccg taaacctgaa tttctcttac caattcttct aaatgcttca tttctcttaa
      361 gtttgctttt attatcatgc tgtgaggacc tgtaacgtga tggcattttg ctatttcttc
      421 cttgttttcg gcaaaatgca aaaaatcctt ctggttttca ggtttaattg atgcatttat
      481 taatacggta ataggcttac ctattttttc gttgtttatt acagccgtat aacgctgaat
      541 tacatccgtt tcttctaatt tttttactct ttctgccacg gcaggcggac tcaatgatac
      601 tattttgcct aattccttca ttgaaattct tccgttattc tgtaatatgc tgataatttt
      661 taaatctgtc tgatccattt tacaccacct tattattatg tatacttaaa aacagattcc
      721 ttttaaaagg gttgcaaaaa ttaaaaaaat taaataatgt acaaagtcat aaaaatatgc
      781 tattgtattt attaaaatga taatcgctga ttatcattat catcaaaata caaaggtatt
      841 ataccataag tgcaataatc atatcaagga aaagaaatga gagataattt taatttaaca
      901 taataaaagg agaaggagaa aatatgacaa aacttaaaat tactgaaaca tctctgagag
      961 atggacatca atcgcttata gcaacaagac tttcaactcc cgaaatgctg ccaattctgc
     1021 aggaaatgga taaggcagga taccatgcaa tggaagtatg gggaggagca acatttgata
     1081 cctgcctcag gtatttaaat gaagaccctt gggaaagact taggaaaatt agagcaaata
     1141 ttaaaaatac aaagcttcaa atgctttccc gcggtcaaaa tttattggga tataaaaatt
     1201 atcctgatga tattgttgaa aggtttcata aaaatgcaat ttctgaagga attgatatag
     1261 tcagagtatt tgatgctctt aatgacataa gaaacttaca aaaaacaata gatacagtaa
     1321 aatccgaagg tgctcacctg cagtgtgcaa tatcctatac aatcagcccc gtacatacaa
     1381 aggagtatta tcttaaattg attaaagact ttgaaaatgc aggagctgac tcagtatgca
     1441 taaaggatat gtccggtata ttgcttcctt atactgctta tgatttagta aaatccataa
     1501 aggaagttac gaacctgcct attgagcttc atacccactg taccggagga atcggggaaa
     1561 tgactgttct aaaagcagta gaagcaggag ttgatattgt tgacacagcc atatcgcctt
     1621 tatcatcagg aacttcacag cctgcaaccg aatccctttg tttgacgctt aaggatacgg
     1681 aaagagatcc acagcttgat ttgatgaagt taaatgaaat tgcagatcat tttaaaaatg
     1741 tcatgaaaaa atttgaagag gaaggaacat taaatacaaa ggtacttatg acagaaccta
     1801 agaccttgtt atatcaaata cccggaggca tgctttccaa cctgcttata caaatgaagt
     1861 ctcttaatgc agttgacaaa tttcaagctg tgttggatga agtgccaaag gtaagagaag
     1921 acttgggatt tcctccgctg gttactccaa tgagccaaat agtaggagct caagcagtgt
     1981 ttaatatagt tacgggagaa aggtacaaag taattcctac ggaaataaga aattatgtaa
     2041 gaggtc
//
LOCUS       DAOSOW010000347         2038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_55112_length_2038_cov_3.095310, whole genome shotgun sequence.
ACCESSION   DAOSOW010000347 DAOSOW010000000
VERSION     DAOSOW010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55112_length_2038_cov_3.095310"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            213..581
                     /locus_tag="PLD67_08220"
     CDS             213..581
                     /locus_tag="PLD67_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22548.1"
                     /translation="MSKSFRKPVFSSRLKRLLDSTVEKCGECCFVHEKFTLNLIKKAT
                     NEPSTDTIFKASEFQGCSSAQIGIAQNSSRFPYGSILVRNFSAVPVSFTVFGDVEEET
                     DMTVQSNSEVAVTLNENLSL"
     gene            complement(653..1084)
                     /locus_tag="PLD67_08225"
     CDS             complement(653..1084)
                     /locus_tag="PLD67_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOT22549.1"
                     /translation="MSNQLTAVATLINDKVKFSGVSRDNKEIIIDYVKPIGDGEGYTS
                     LELFLISLATCSGTSVSSLLRKMNKDVKGLKITTYGERREEHPTYFKKILIHFELKSK
                     DAEAPDMEKAIRISEESICPVWNMVKNNVEINYEYEIIRVD"
     gene            complement(1161..1940)
                     /locus_tag="PLD67_08230"
     CDS             complement(1161..1940)
                     /locus_tag="PLD67_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOT22550.1"
                     /translation="MELNFDKVYDEQYDKLYKLAYRLTANKQEAEDVLQEAFLNAYSA
                     FPKFQNKSQISTWLYRIVVNCSYKNMKKLKRLPVYDIAPRYNMTKDEFFEKLKSYEPV
                     EDEVMVNSMREQCLQLFLKCMPSKQRIAFVLKILLGLPCEDVAQIMDISVGAVKTNVY
                     RARLRMKENMEDKCSYINPDSPCNCKNWVAYAIENNRMDMIPSFPLDKEIDYNLIFTK
                     EMNFLSKIMFLYDKYPERMPYGSFFANIKTMISKKSLEILS"
BASE COUNT          623 a          409 c          313 g          693 t
ORIGIN      
        1 cgcttgaatg gggttcaaga ggtcgcaggt tcaattcctg tcacccagac catctaagta
       61 atcagagtgc tttagggcac ttttttattt tttaagaaaa gctgtattag gcttttcatg
      121 actttttata actacaaaca caccaaatat acactgcctt aatactatat attaagaaaa
      181 gtagacaagg tatcccaaaa ggaggtgtat aaatgagtaa atcatttaga aaaccagttt
      241 tttcatctcg tctcaaacga ttgctggaca gtacagtaga aaaatgcggt gaatgctgtt
      301 ttgttcacga aaagtttacc ttaaacctga taaaaaaagc aacaaatgag ccaagtacag
      361 atacaatctt taaggcaagt gaatttcaag gttgttcttc agcacaaatt ggtatagcac
      421 aaaattcttc ccgattccca tatggcagta tattagtaag aaatttttca gcagtacctg
      481 tctcctttac agtattcggg gatgtggaag aagaaaccga tatgacagtt caatctaatt
      541 ctgaggttgc agtaacactt aatgaaaatt taagcctgta atcgcaatga tttcaggctt
      601 attttacgga ttttatcata ttaattaaat aaaattttaa agcatattac acttaatcaa
      661 ctctgattat ctcatactca tagtttatct ctacattatt tttcaccata ttccacacag
      721 ggcaaatgct ttcttcagat atcctgatag ccttttccat atcaggagct tcagcatcct
      781 tcgacttaag ctcaaaatgt atgagtatct ttttaaaata ggttggatgt tcttccctcc
      841 tctctccgta agttgtaatt tttagtcctt taacatcctt gttcatcttc cttaacagtg
      901 aagagactga tgttccgctg caggtagcaa ggcttatcag gaacagttca agggatgtat
      961 atccttcacc gtcgccgata ggctttacat agtcgataat tatctcctta ttatcccttg
     1021 aaacgcccga gaatttcacc ttatcattta taagtgttgc aactgctgtt aattgattgc
     1081 tcataaaaaa cctcctgtta ttttttgatt cattagcttt gatactaaaa ataacaaaag
     1141 gtcacatcga ttttttattt ttatgacaaa atttctaaag attttttaga aatcatagtt
     1201 ttaatatttg caaaaaagct gccataaggc attctttccg gatatttatc atacaagaac
     1261 attatttttg aaaggaaatt catctccttc gtgaatatta aattataatc tatttcttta
     1321 tccagcggaa agcttggtat catatccatt ctattgtttt ctattgcata tgcaacccaa
     1381 ttcttgcagt tacaagggct gtcggggttt atgtatgagc atttatcctc catattttcc
     1441 ttcattctaa gccttgccct atagacattg gtttttaccg ctccaactga aatatccata
     1501 atctgcgcaa catcctcaca aggcaaccca agcaatattt taagcacaaa agcaatccgc
     1561 tgcttgcttg gcatgcattt caaaaaaagc tgcaggcatt gttctctcat agaatttacc
     1621 ataacctcat cctcaacagg ctcataggat tttagctttt cgaaaaactc atccttggtc
     1681 atattgtatc ttggagcaat gtcataaaca ggcagcctct tcaacttctt catattctta
     1741 tatgaacagt ttacaacaat cctgtataac caggttgata tctggctttt attttgaaac
     1801 ttcgggaagg cactataggc atttaaaaat gcttcctgca gcacatcttc agcttcttgc
     1861 ttatttgctg tcaatcgata agccagttta tacaatttgt catattgctc gtcatatact
     1921 ttatcaaaat taagctccat aaaattctct ccttcagcaa tagtaaaacc tttttcacaa
     1981 ttgaagctcc tcaggctgcc taagcctctg atgccttagg acgggaatct gatacttc
//
LOCUS       DAOSOW010000348         2035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_55223_length_2035_cov_3.549495, whole genome shotgun sequence.
ACCESSION   DAOSOW010000348 DAOSOW010000000
VERSION     DAOSOW010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55223_length_2035_cov_3.549495"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="PLD67_08235"
     CDS             complement(<1..1280)
                     /locus_tag="PLD67_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOT22551.1"
                     /translation="MKNKISHQFLLNYLTFFLMSLLAALFAYLLLSFADDVISKTLVK
                     NRYPSELIMKDNYADIDAESVVEHGGGVQVINENYEVVYSKGLDTLGKKKFSVSEFTD
                     FLVKSKEKGFKYHYDILYNPQAKFWLIVTFPTSMRLDISFVYNKEAASEDLKNVSFVF
                     ISVFIFYLLLLGLLAFIFSKITSIHIIKPLKMLKEGTKALREGDYSARVTMNLKNEFA
                     ELQDTFNEMAEKIETEIKLREQSEAERKKIIMDISHDLKNPLAIVVGYTELCLKKAES
                     LDKELHNYLQIIFKNSKRAGVLLNDLFEFSKLESSEFSMKFHKTDICEYLRQACGDIL
                     PMIEQAGFEYSFDIPEKAVYVMLDVSQMNRVLFNLAENAIRYNPTGANLSVSLYDDTT
                     NVIINFSDDGIGIPSHLAKNIFKPFVRIDDSRNS"
     gene            complement(1270..1977)
                     /locus_tag="PLD67_08240"
     CDS             complement(1270..1977)
                     /locus_tag="PLD67_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT22552.1"
                     /translation="MNILVAEDEIDIRNLIKITLEDEGYSVITACDGIEALKKLKETP
                     VDLAILDIMMPRLDGFNLLRKIRENSTIPVIFLTARTDDLDKVLGLELGADDYIAKPF
                     SMSELIARVRAQLRRNNEYMLPKQENKTLLQYGDLTIDKESCCAYKNNEQIELNAKEY
                     KLLLHLMENPERVFTKKQLYNAVWDDDYYYDDNTIMVHISHLRNKIETDSQNPEYIKT
                     IRGLGYKLHKTGGYNEK"
BASE COUNT          620 a          383 c          301 g          731 t
ORIGIN      
        1 tgagaattac gagaatcatc aatcctgaca aagggcttaa aaatattttt tgcaagatgc
       61 gacggaattc ctataccatc atcactaaaa ttaattatta catttgttgt atcatcatat
      121 aaactcactg ataaatttgc tcccgtcgga ttgtatctta ttgcattttc agctaaattg
      181 aatagtacac ggttcatctg gctgacgtcc agcatcacat ataccgcctt ttcaggtata
      241 tcaaagctgt attcaaagcc ggcttgttct atcataggca gtatatcgcc gcatgcttgc
      301 cttaaatatt cacatatgtc ggttttatga aatttcatgg agaattcaga gctttccaac
      361 ttagagaatt caaacaaatc atttaacaaa acacctgctc ttttactgtt tttaaagata
      421 atttgaagat aattatgcag ctctttatct agtgactctg cttttttaag acatagctcc
      481 gtatatccga caacaatagc cagaggattt tttaaatcat gagaaatatc cataattatt
      541 ttcttgcgtt cagcttccga ttgttctctc agctttattt cggtctcaat tttttcagcc
      601 atttcattaa aagtatcctg caattctgca aattcatttt ttaaattcat ggtaactctt
      661 gcagaataat caccttctct taatgcttta gtcccttcct ttaacatttt taagggttta
      721 attatgtgta tcgatgtaat ttttgagaaa ataaaggcaa gcaacccaag cagaagcaaa
      781 tagaaaataa acacggaaat gaatacgaag gatacatttt ttaaatcctc cgaggcagct
      841 tccttattgt aaacaaagga aatatctaaa cgcattgatg tcgggaatgt tacaataagc
      901 caaaactttg cctgcggatt atatagaata tcataatgat acttaaatcc tttctctttg
      961 ctttttacaa gaaaatcagt aaattcactc acagaaaact tcttctttcc aagagtatct
     1021 aatccttttg aatacaccac ttcatagttt tcatttatta cctgtactcc accgccatgc
     1081 tcaactactg attctgcatc aatatcggca taattgtctt tcatgatgag ttctgaaggg
     1141 tatctgtttt ttacaagtgt ttttgaaatc acatcatctg caaacgacaa caataaatag
     1201 gcaaataaag ctgctaataa tgacataaga aaaaatgtaa gataatttaa caggaattga
     1261 tgggaaattt tatttttcat tatatccacc tgttttatga agcttatatc ctaagcctcg
     1321 tatagtcttt atatattcgg gattttgaga atcagtttct attttattgc gcagatgact
     1381 gatatgaacc atgattgtat tatcatcata gtaatagtca tcatcccaaa ctgcattgta
     1441 gagctgtttt tttgtaaata ctctctcggg gttttccatt aaatgtaaga gcagtttgta
     1501 ttcctttgca ttgagttcta tttgctcatt atttttgtaa gcacagcaac tttctttatc
     1561 tatcgttaaa tcaccatatt gaagcaaggt tttattttct tgtttgggca gcatgtattc
     1621 attatttctc ctaagttgag ccctgactcg tgcaatgagc tcactcatgg agaaaggctt
     1681 ggcaatgtaa tcatcagctc ctaattcaag tcctaatacc ttgtccaaat catctgttct
     1741 tgcagtaaga aatataacag gaatggtgct gttttctctt atttttctaa gcaggttaaa
     1801 tccatcaagc cgaggcatca taatatctaa tatggctaag tcgacaggtg tttccttcag
     1861 tttttttaac gcctcaatac cgtcgcatgc ggtaattacg gaatagccct catcctccaa
     1921 ggttatcttt ataaggtttc taatatctat ttcatcctct gccactaata tattcaattt
     1981 tatcccctct tttatgaatt ttatatattt tcaatatagc acaatattta tgttg
//
LOCUS       DAOSOW010000349         2020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_55823_length_2020_cov_3.274809, whole genome shotgun sequence.
ACCESSION   DAOSOW010000349 DAOSOW010000000
VERSION     DAOSOW010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55823_length_2020_cov_3.274809"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLD67_08245"
     CDS             complement(<1..405)
                     /locus_tag="PLD67_08245"
                     /inference="COORDINATES: protein motif:HMM:NF017648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HOT22553.1"
                     /translation="MSNLSDIIESFIKELLDANNNDVIEIQRNILAQQFDCSPSQINY
                     VLTTRFTNDRGYVVESRRGGGGYIRIFRVRSSMEDELKRILNETIGDSITLNKSIDLI
                     NALMEREVITEGERAIMQSVLSDRVLNIVAYED"
     gene            615..899
                     /locus_tag="PLD67_08250"
     CDS             615..899
                     /locus_tag="PLD67_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HOT22554.1"
                     /translation="MYGIHTHGFEGPTTINDNHRHFYEGISSADPNIPGHVHNITATT
                     TFADGHRHSFSVNTAPAVPVRQGHIHPYMGTTTFDDGHVHYFKGNTTVSR"
     gene            complement(990..1694)
                     /locus_tag="PLD67_08255"
     CDS             complement(990..1694)
                     /locus_tag="PLD67_08255"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOT22555.1"
                     /translation="MSVSGNERNSCLTIGRMAPDFTALSTDGIVTLSQYRGKWVVIIS
                     EPGNFASTSTSSLIALSTYFDEFAKRNVQILVVTIDNNFSNIEWKMNIFNTYGIIVPF
                     PILEDRDAQIANMYGMVNPDRIYEESVRDIFIINPQGRIKAILTYPVSCGRNTFEILR
                     IIDSLQLTDAYNVYTPANWMPGDPVVVPTTQNFNEALLRQQGQSGFNCPNWYTCYLDY
                     NSLTQRDNISELKKRS"
BASE COUNT          659 a          374 c          296 g          691 t
ORIGIN      
        1 gtcttcataa gcaacaatat taagaaccct gtcacttaat acactttgca taattgctct
       61 ttcaccttcc gttattactt ctctttccat cagtgcattg attaaatcaa ttgatttatt
      121 taatgttatg ctgtctccta tagtttcatt taatatgcgc ttgagttcat cctccataga
      181 agagcgaacc ctaaaaattc gtatatatcc tcctccgcct cttctgcttt ctacaacata
      241 tcccctgtca ttggtaaagc gagtagtaag aacgtaatta atctgagatg gagaacaatc
      301 aaactgctgt gccaaaatat ttctttgtat ttcgattaca tcattattgt ttgcatccaa
      361 taattctttt ataaagcttt caattatatc acttaaattt gacattccgc acctccttac
      421 tcttctatag tttgtcttta actgtctttg actttgacta tctttgacct taaacttatt
      481 ttaacactat taaaaataaa atgcaagggg ttttttgaaa aaaataacaa ttaaaacgta
      541 gtctacagta acaaagcata ttataggaca taaaatactg taagcaatta atttattata
      601 gaggtgatta tataatgtat ggtattcata ctcatggatt tgaaggtccc acaaccataa
      661 atgacaatca tagacacttt tacgaaggaa tatcttctgc agaccctaat attccggggc
      721 acgttcataa tataactgct acaacaacat ttgctgatgg gcacagacac agcttcagtg
      781 taaacacggc tcctgcagtt ccagtaagac aagggcatat tcacccgtat atgggaacaa
      841 caacctttga tgacgggcat gttcattatt ttaaagggaa tactacagtt tcaagataaa
      901 aataataatc ggcatcattt tatgaagccg atttttttgt gccttttatc ctgaacaata
      961 attagaaatc taagatcagt tttatataat caacttctct tcttcagctc agaaatatta
     1021 tctcgctgag ttagagaatt ataatctaaa taacaagtgt accaattagg acagttaaat
     1081 ccgctttgtc cttgctgtct taacaaggcc tcattaaaat tttgggtagt tggcaccaca
     1141 acagggtctc cgggcatcca gttggcaggt gtgtagacat tgtaagcatc cgtcaattgt
     1201 agagaatcaa taatcctcaa aatttcaaat gtattcctgc cgcaagaaac cggataagtc
     1261 aatatagctt ttattcttcc ttggggattg ataataaaaa tatcccttac actttcttca
     1321 tatattctgt caggattaac cattccatac atatttgcta tttgagcatc tctatcctct
     1381 aaaatcggaa atggtactat tattccatat gtgttaaaaa tattcatttt ccactctatg
     1441 ttcgaaaaat tgttatctat tgttacaact aatatttgga catttctttt agcaaattcg
     1501 tcaaaatacg tagacagggc tataagagat gaggttgatg tagaggcaaa atttccgggc
     1561 tcgcttataa tcactaccca tttccctctg tattgcgaca atgttacaat accgtctgta
     1621 gaaagtgcag tgaagtcagg agccattctc cctattgtta agcaagagtt tctttcattt
     1681 ccgctaacac tcatagtatc ctaacggcct attcaataat aaatatgcct ctcctttctc
     1741 cttttatatc caatattata tgtaatccta tttaatttgt gagtatgtaa tgtatcctga
     1801 tataaatagc agtaatatta tttcaaacga aatttggttt aaattaataa cttgatggta
     1861 ttataatatt atcggataaa atttactcta tacaaatata ggaggcataa gatatgaacg
     1921 cacaaaaatt tactcaaaaa tcattggaag caattcaaga tgcacacata cttgttttta
     1981 aaaaccaata aataggtatt tgtgaggtac tttaaatgaa
//
LOCUS       DAOSOW010000350         2012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_56102_length_2012_cov_4.451712, whole genome shotgun sequence.
ACCESSION   DAOSOW010000350 DAOSOW010000000
VERSION     DAOSOW010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56102_length_2012_cov_4.451712"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..1231
                     /locus_tag="PLD67_08260"
     CDS             140..1231
                     /locus_tag="PLD67_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT22556.1"
                     /translation="MKKFLSVILTLALLLTLFPLTALASEPFSLEAPKNLIGELKFRD
                     DGIPYFELKLTVPESVKTINSNLVDNPDYYDGISCDGIEIIFDYKYGNYDWNEGPSLY
                     WNTSMAVSDYLELGAYEYEPFDEGDFDTVDIKNEVYQFRARFNALWGYQGDWIDKDIY
                     SEYSNIVTIGNPAFYDDASDWAVVELEKANEAGLIPDILKGKDLTKSITREEFAELAL
                     LLYEKTTDKTVASVSENPFTDTTNPQIVKAFSIGITQGTSATTFSPNELINREQCATM
                     LFRTIKAIEPEGDFSIEGVKDFPDQADISSWAVEGTKFMAKLGIIKGDSNGNFMPKAV
                     TDAQKAVGYGMASREAAILMSVRTYEEYK"
     gene            1362..1700
                     /locus_tag="PLD67_08265"
     CDS             1362..1700
                     /locus_tag="PLD67_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtrH family sporulation protein"
                     /protein_id="HOT22557.1"
                     /translation="MSSFYSSIVHNFLIAFGVVIGASLFSGLGALMTGTPPLKVMTDV
                     AGSIKLWAIAASLGGTFSSFLVIEKGLFEGEIKTLLKQVFYIVISLTGANMGYEFIRL
                     LQRCGNIWGK"
     gene            1697..>2012
                     /locus_tag="PLD67_08270"
     CDS             1697..>2012
                     /locus_tag="PLD67_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22558.1"
                     /translation="MMRVKGNVKNTAGYKYFICFITGLLWGTVIGSLGISILVSHRME
                     ELYEKIAYLEHTIQDKDEKLEKLESSINNANIVLKDISVILEFTGLSKEQIDLIDNIE
                     IEK"
BASE COUNT          696 a          295 c          413 g          608 t
ORIGIN      
        1 tgcatacaga ttgggtttaa taaaagaaca ggggtgatta ataggaaaaa tcaccctcag
       61 ggtgattgaa ataattgttg attccaatat aattaatata aatattataa ataatataaa
      121 taattataag ggggttacaa tgaaaaaatt tttatctgtc attttaactc tggcactttt
      181 attgacatta tttccattga cagcattggc aagcgagcct ttttctttgg aagcaccgaa
      241 aaatcttata ggtgaattaa aatttaggga tgacggtatt ccttattttg aattaaagct
      301 taccgttccc gaaagtgtaa aaacaattaa ttctaatttg gtagataatc cggattatta
      361 tgacggtata agttgcgatg ggattgaaat tatttttgat tacaaatatg gcaattacga
      421 ttggaatgaa ggtccctcat tatactggaa tacttcaatg gctgtttctg attatcttga
      481 acttggtgct tatgaatatg aaccatttga tgaaggagat tttgataccg ttgatatcaa
      541 aaacgaagtt tatcagttcc gtgctcgatt caatgcactt tggggctatc agggcgattg
      601 gattgacaaa gatatttatt cagagtactc aaacatagtt acaataggta acccggcatt
      661 ttatgacgat gccagcgatt gggcagtagt tgaattggaa aaagccaatg aagcaggact
      721 tattcctgac atattgaagg gaaaagattt gacaaaatca ataacccgtg aagaatttgc
      781 tgagctggct ctcttgcttt acgaaaaaac tactgacaag acagtggcaa gcgtatctga
      841 aaatccattt accgatacga ctaatcccca gattgtaaaa gcattttcta taggaattac
      901 tcaaggaaca tcagcaacta cattttcacc aaatgaatta attaatcgag aacaatgcgc
      961 aaccatgctt tttagaacaa taaaagcaat agagcctgaa ggtgacttta gtattgaagg
     1021 tgttaaggac tttcctgacc aagctgatat ttcatcatgg gcagtggaag gaaccaaatt
     1081 tatggctaaa ctgggaataa taaaaggtga cagtaacggt aattttatgc ctaaggctgt
     1141 gacggatgct caaaaagctg tcggttacgg catggcatca agagaagcag caattcttat
     1201 gtctgtcaga acatacgaag aatataaata aatacactgt tattgtaatg aattcgattt
     1261 gaaggctcag acctcgtaac tctgaactca ttgaagaaac taaacaatca atcataaaga
     1321 catttttcac tcatattata aaaagagagt atggtgataa aatgtcttct ttttattcaa
     1381 gtatagtgca taacttttta attgcatttg gagtcgttat aggagcaagc ttgttttccg
     1441 ggttgggagc tcttatgaca ggcacacccc ctttaaaggt gatgacagat gttgcaggtt
     1501 caatcaaatt atgggctata gcggcatccc tgggtgggac cttttcatct ttcttggtaa
     1561 ttgaaaaagg gctgtttgaa ggagaaataa aaacattgtt aaaacaagta ttttatatag
     1621 tgatttcatt aaccggcgcc aatatgggtt atgaatttat caggcttctt caaagatgcg
     1681 gcaatatatg ggggaaatga tgagggttaa aggaaatgta aaaaacactg caggatataa
     1741 atattttatt tgctttataa cgggactgct ttggggaaca gttataggct ctttgggtat
     1801 aagtatatta gtcagccata gaatggaaga gctttatgaa aagatagcat atttggagca
     1861 taccattcaa gacaaagatg agaaactgga aaagcttgaa agttcaatca acaatgcaaa
     1921 tatagtgctg aaggatattt ccgtcatatt ggaatttacc ggtttaagca aggaacaaat
     1981 agacctgata gacaatattg aaattgaaaa ag
//
LOCUS       DAOSOW010000351         1987 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_57022_length_1987_cov_3.977743, whole genome shotgun sequence.
ACCESSION   DAOSOW010000351 DAOSOW010000000
VERSION     DAOSOW010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57022_length_1987_cov_3.977743"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..321)
                     /locus_tag="PLD67_08275"
                     /pseudo
     CDS             complement(1..321)
                     /locus_tag="PLD67_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175190.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
     gene            complement(524..>1987)
                     /locus_tag="PLD67_08280"
     CDS             complement(524..>1987)
                     /locus_tag="PLD67_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018999429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT22559.1"
                     /translation="ISIKLIVYFSAALIVFSIIIGNIFMLLFKNNAVEIHKNELKRRA
                     LTIADTISELMDGSSLNFGPGMGRHGGMMMGMQGGLGLYIRNLDDIAMADAWIVDEEL
                     NLLITGSMSHIQYNYTDLPQDAGIVVKKVFEGQTTFSEGFSKLLETPTLTIGTPITSD
                     GEIIGALLIHSPIQGINDATSEGFRILLISIFMALILSVLLSVALALTFTKPLEKMKN
                     TAALLAGGNYSVKTGIKQNDEIGDLAKTMDILSQRLGKAEEERKRVQKQRQDFITNIS
                     HELRTPVTVIRGSLEALSDGVVTDSEMINEYYKQILNESKSLERLINDLLELSRLQNV
                     DFKIEMQELNICDVLKDSLRSASNLARQKNINIQYNSDKDVFLTKGDYGRLRQMFLTV
                     IDNAIKFSHESKSIYITLKDNQITIRDEGIGISEKDLPNIFDRYYSVKSDENKTGTGL
                     GLTIAKQIALRHDIELFAESKINEGTSFVFVIRSNFE"
BASE COUNT          584 a          402 c          282 g          719 t
ORIGIN      
        1 accaatcatc aagcaaaaat ccaagatggc tgcaggaagt acaccaacta ttaccttaaa
       61 ccatatatcc attgtatctc ttttttgttt tggtgtcttg cttggagcaa atggattcag
      121 tttatggaag tatagcaata caacggccat aatggctccc aattgtataa caactctgaa
      181 catttccata aattcatcgg aagcattaaa ttttataaat tcctcaacca aaatcatgtg
      241 accggtgctg ctaataggaa gccattcggt tattccctct actattccca atattattgc
      301 tttgattatt tctatgtaca ttcgtcctcc aaccctctta attttataac acatatgaaa
      361 ttccgcagtc tcgttgcaca attttgtttg ctcctttcgt cgcacaaaat tggcactcgt
      421 tgcgaatgac ctaccgctat ttttctctcc ctttagtcgg aaaaataggg ttagggcatc
      481 tttattatat attttgttaa ttaaattttc aataaaatat aatttactca aagttgctac
      541 ggataacaaa tacaaaactt gttccctcat taatcttgct ttctgcaaaa agctctatat
      601 cgtgccttaa agctatttgt tttgcaatag taagtccaag tcctgtacct gttttgtttt
      661 catctgattt tacactgtaa tacctgtcga atatattggg aaggtctttt tcggatatgc
      721 cgattccttc atctcttata gtaatttgat tgtcttttaa agttatataa atacttttac
      781 tctcatgtga aaatttaatt gcattatcta taactgtcaa aaacatttgg cgaagtcttc
      841 cgtaatctcc cttggttaaa aacacatcct tatcggaatt atattgaata ttaatatttt
      901 tttgtcttgc caagtttgaa gcacttcgta aagaatcctt taatacgtca caaatattca
      961 gctcttgcat ttctatttta aagtcaacat tttgcagtct tgacaattcc aataaatcgt
     1021 ttatcaatct ttccaaactt ttgctttcat ttaatatctg tttataatat tcattaatca
     1081 tctccgaatc ggtaactact ccgtcggaaa gcgcctcaag ggagccccta ataacagtta
     1141 cgggagttct taattcgtga gaaatatttg ttataaaatc ctgcctttgt ttttgcactc
     1201 tttttctttc ttcttcagct ttaccgagcc tttggctcaa tatgtccatt gttttagcaa
     1261 ggtcacctat ttcatcattt tgtttaatac cggtttttac gctgtaattc cctcctgcca
     1321 gcagggctgc cgtatttttc atcttttcaa gcggtttggt aaatgttaaa gctaaggcaa
     1381 cagataaaag tacagataaa attaatgcca taaaaatact tattaataaa atcctaaaac
     1441 cttcacttgt tgcatcattt ataccttgaa taggcgaatg tattaaaagt gctcctatta
     1501 tttcaccatc agaagtaatt ggagtgccta tcgtcaaggt aggtgtttcc aaaagtttgc
     1561 tgaacccttc actgaaagtt gtttggcctt caaaaacttt tttaacaaca atacctgcat
     1621 cttgaggcag gtcggtataa ttgtactgta tgtgggacat gcttccggtt attaaaaggt
     1681 ttaattcttc atcaacaatc catgcatctg ccatggctat atcatccaaa ttccgtatat
     1741 ataaacctaa tccgccctgc attcccatca tcatgccacc atgtctgccc attccgggtc
     1801 cgaaatttaa actgcttcca tccattaact ccgaaattgt atctgcaatt gtaagagccc
     1861 gtctttttag ctcattttta tgaatttcta ctgcattatt tttaaacaaa agcatgaata
     1921 tattgcctat aattattgaa aaaacaatta atgcagctga aaaatacact atcagtttta
     1981 ttgaaat
//
LOCUS       DAOSOW010000352         1981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_57286_length_1981_cov_3.179647, whole genome shotgun sequence.
ACCESSION   DAOSOW010000352 DAOSOW010000000
VERSION     DAOSOW010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57286_length_1981_cov_3.179647"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="PLD67_08285"
     CDS             complement(<1..757)
                     /locus_tag="PLD67_08285"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOT22560.1"
                     /translation="MDYKELALELHKNNIVVDSHLDLAGEIYNRNRAGEKEIIKNHYL
                     ESFKKGGFNLIISSLYIDDLFLPEMALRIALGQIRALIEDVESCHGEVMLVKSREDLN
                     KAVNEDKIGIIISFEGLEPIGSDIGLLRIFYELGVRAAGLVWSRRNYVADGCSFFPVE
                     EGQRGGLTRFGVKVVNKMEEMNMLIDVSHLNDEGFLDVLKYTKKPFIASHSNSRTIHG
                     SMRNLSDEHIRAIADRNGVIGINAIKSIAGVKDG"
     gene            complement(813..1901)
                     /locus_tag="PLD67_08290"
     CDS             complement(813..1901)
                     /locus_tag="PLD67_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HOT22561.1"
                     /translation="MKIKYLNKLVEILKKKEIDAILIAPSEEMEFIMGHNTHLCERFQ
                     ALVIKSDGNYFYICNLLTVDEIQSVLGPNIKVYGWFDGDVFTDTVKKAFEEQGLIGKT
                     IAVNSTERAFIILQILEAMDVKFINGKTVLEDMRIIKDEEELNNLRMAARITDESYEE
                     LLKFIKPGIKEADIARKMNEIFKEKGADEGFTMVCSGPNSSYPHYNDDQRVIQEKDII
                     VLDWGCKYNNMCADMSRTVFVGGITEEERKVYEIVKASQEAGEKAAVKGAYIPDVDKA
                     ARDIIEKAGYGKYFFTRLGHGIGYSVHEAPDIKASNKRNLEKGMVFSIEPGIYMAGNF
                     GIRIENIVAVTDDGNEILNKSTKEIMII"
BASE COUNT          538 a          468 c          250 g          725 t
ORIGIN      
        1 caccatcttt tactcctgca atacttttga ttgcatttat tcctataaca ccatttctat
       61 ctgctatagc cctgatatgt tcgtccgaca aattcctcat acttccatga attgttcttg
      121 agttcgaatg agaagctata aaaggctttt tggtgtattt gagtacatcc aggaaacctt
      181 cgtcgtttag gtggctgaca tcaataagca tattcatttc ttccatttta ttgacaacct
      241 tgaccccaaa ccttgtaagc cctcctcttt gaccttcttc aaccgggaaa aaactgcaac
      301 cgtcggcaac ataatttctc ctgctccaca ccaatcctgc agcccttact ccaagctcat
      361 aaaatatcct taaaagaccg atatcgcttc ctatcggctc caatccttcg aaggagataa
      421 ttataccgat tttgtcttca tttactgcct tgtttaagtc ttccctgctt ttaaccagca
      481 ttacttcgcc atggcaagat tccacatcct caattaatgc tcttatctgt cccaatgcaa
      541 tccttaaagc catttcaggc aaaaataagt catcaatata taaagatgat ataattaaat
      601 taaagccgcc ttttttaaaa ctttcaagat agtgattttt aattatttcc ttttccccgg
      661 ccctgtttct gttgtaaatt tcccctgcca ggtctaaatg tgaatccact acaatattat
      721 ttttgtgcaa ttctaaagca agctctttgt aatccatttg ctctcctctt gtaaataatg
      781 caaaatgaca gacagtattt aagtacaata atttatatta tcattatttc ttttgtggat
      841 ttatttaata tttcatttcc atcgtcggtt actgctacaa tattttctat acgtattccg
      901 aaatttccgg ccatataaat acccggctcg atgctgaaca ccatgccttt ttctaaattc
      961 cttttattgc ttgctttaat gtccggagct tcatgtacgg aatatcctat tccatgtcct
     1021 aatcttgtaa aaaagtactt tccataacct gctttttcga ttatatctct tgcagcctta
     1081 tccacatccg gtatgtatgc accttttaca gctgcctttt cccctgcttc ctggcttgcc
     1141 tttactattt cataaacctt tcgctcttct tcggtaattc ctccaacgaa tacggttctt
     1201 gacatatcgg cacacatatt attgtattta catccccagt caagcacaat aatatctttt
     1261 tcttgaatta ccctctggtc atcgttgtag tgggggtaag atgaatttgg tcctgaacag
     1321 accattgtaa acccttcatc agcacctttt tctttgaata tttcattcat tttcctggct
     1381 atgtcagctt cctttattcc cggttttata aatttcaaca attcttcata ggactcatct
     1441 gtaatacgtg cagccattct taaattgtta agctcttctt catcctttat aatcctcata
     1501 tcttccaata cagtcttgcc gtttataaat ttaacatcca tagcttccaa tatctgaaga
     1561 attataaatg ctctttcggt agaatttact gctatagtct tcccaatcaa tccttgctct
     1621 tcaaatgcct ttttaaccgt atccgtaaac acatctccgt caaaccagcc gtagactttt
     1681 atatttggtc ccaaaacact ctgaatttca tctaccgtca gcaaattaca aatgtaaaag
     1741 taatttccgt cactttttat aaccagtgct tgaaaccttt cacaaagatg agtattgtgc
     1801 cccattatga attccatttc ttccgaagga gcaattaata tagcatcgat ttctttcttc
     1861 tttaatattt caaccaactt gtttaaatat ttaattttca caatatcctc ccataatttg
     1921 tcattgtaaa ttgtcatttt gagagcaact ttcgcatatg ctcaggatga caaaaacctt
     1981 t
//
LOCUS       DAOSOW010000353         1975 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_57525_length_1975_cov_4.923438, whole genome shotgun sequence.
ACCESSION   DAOSOW010000353 DAOSOW010000000
VERSION     DAOSOW010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57525_length_1975_cov_4.923438"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>241)
                     /locus_tag="PLD67_08295"
     CDS             complement(<1..>241)
                     /locus_tag="PLD67_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOT22562.1"
                     /translation="YRLDVFLHNKGYVGSREKAKELILNNSVFVDDKLITKPSYMVND
                     DVKIKITEDVYISRGYLKIEKAIHVFNINIENKTAV"
     assembly_gap    242..251
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(431..625)
                     /gene="xseB"
                     /locus_tag="PLD67_08300"
     CDS             complement(431..625)
                     /gene="xseB"
                     /locus_tag="PLD67_08300"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOT22563.1"
                     /translation="MEYDNFESALEELKNIVRKFENKDDISIDELLENYEKGMKAYSF
                     CMKKLDDTQRKIKIIDENIE"
     gene            complement(627..1808)
                     /gene="xseA"
                     /locus_tag="PLD67_08305"
     CDS             complement(627..1808)
                     /gene="xseA"
                     /locus_tag="PLD67_08305"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOT22564.1"
                     /translation="MKLKPIKVSVLNQYIKKYLMSNSILNNLRVEGEITNIRISKTGY
                     TYFSLCDEISCISCVAFFADTISKNGDKIIAEGELSVYEAKGTYQLIVRDIERAGLGK
                     ILLDLELLKEKLKEQGLFDRKRELPEFPQRIGIITSKTGAAIKDILKTFENVKANIDI
                     TIYNTLVQGEKSKKSIIEGLKYFNSVETDVILISRGGGSFEDLNVFNDMDVAMEVYKS
                     NIVVVTGIGHETDRTLTDYVADVYCHTPTAAAERIIRNYKDIEEKLDNLVFTLKSETN
                     NTISLKKAELISNKYLLRSSLPLEDIYKTKNQLDTYKNLIKNKTTSQINEYYFNLKIM
                     LEKLNTFDYKKQLEKGFALVSDNSGNIIKHKEQVELKDILHIRFADFKIEAETRDIKE
                     V"
     gene            complement(1789..>1975)
                     /locus_tag="PLD67_08310"
     CDS             complement(1789..>1975)
                     /locus_tag="PLD67_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22565.1"
                     /translation="QHKTLIISGGVASNSYIRNYITKSVKGYEVLFPAVNNSSDNALG
                     LAYLPIIDRWHNEIKTN"
BASE COUNT          589 a          341 c          241 g          794 t
ORIGIN      
        1 actgctgtct tattttcaat attaatgtta aatacatgaa ttgctttttc aatttttaaa
       61 taacccctac ttatatatac atcttctgta atttttattt taacatcatc atttaccata
      121 tatgatggct ttgttattaa tttatcatcc acaaatacag aattgtttaa aataagctcc
      181 tttgcctttt cacggctgcc aacataccct ttattatgca aaaatacgtc aagtctgtaa
      241 tnnnnnnnnn nacataaata ttcttctaaa aaaataaata tttggatatt caataaaatt
      301 atagcatatc aattaactta tatcaattaa tatttttaat aattattaaa atttaaggat
      361 acacctttat ggaagtcagc ctataataca ggtcttcaaa ggaatgttcc ctcttatgtc
      421 taaggatttc ttattcaata ttttcatcta taattttgat ttttctttga gtgtcatcca
      481 actttttcat gcaaaaagaa taagccttca ttcccttttc ataattttcc agaagctcgt
      541 caattgaaat atcatcttta ttttcaaatt tccggacaat attttttaat tcttccaagg
      601 cactttcaaa attatcgtac tccatattac acctctttta tatctcttgt ttctgcttct
      661 attttaaagt cagcaaatct tatatgaaga atatctttta attctacctg ttctttatgc
      721 tttataatat ttcctgaatt gtccgatacc aacgcaaatc ccttttcaag ctgtttcttg
      781 taatcaaaag tattcaattt ttctaacatt attttaagat tgaaataata ctcattaatt
      841 tgtgaggtag ttttattttt tattaaattt ttataagtat ctaactgatt ttttgtttta
      901 taaatatcct ctaagggcag gctgcttctt aataaatatt tattagatat tagttcagcc
      961 ttttttaatg atatggtatt gtttgtttct gatttcaagg taaatacaag attatcgagc
     1021 ttttcttcaa tatctttgta gtttctaata attctttcgg ctgcagcagt cggagtatga
     1081 cagtatacat cagccacata gtcggtcagt gttctgtctg tttcgtgtcc tattccggta
     1141 acaaccacaa tattggattt ataaacttcc attgctacgt ccatatcgtt aaaaacattt
     1201 aaatcctcaa aacttcctcc tcctcttgaa atgagaatta cgtcagtttc cacactgtta
     1261 aaatatttta atccttcaat aatacttttt ttggattttt ctccttgtac caatgtgttg
     1321 tatatagtta tatcaatatt ggcttttaca ttttcaaatg tttttaatat atccttgatg
     1381 gctgccccag tctttgaggt aattatgcct attctttgag ggaattcggg aagctctctt
     1441 ttgcggtcaa agagtccctg ctcttttagt ttttctttta atagctccaa atctaaaaga
     1501 attttaccta atcctgccct ttcaatatct ctaacaataa gctggtaggt accctttgct
     1561 tcgtatacgg aaagctctcc ttccgctatt attttgtccc cgttttttga aattgtgtcg
     1621 gcaaaaaaag caacacaaga tatacaggat atttcatcgc ataatgaaaa ataagtatag
     1681 cctgtcttac ttattctaat gtttgtaatt tcaccctcaa ccctcaaatt attcaggatt
     1741 gaattactca ttaagtactt ttttatatat tggtttaaaa cagaaacttt aattggtttt
     1801 aatttcattg tgccacctgt caataatggg gagatatgca agacctaatg cattgtcgga
     1861 agaattattt acagcaggaa ataatacttc atatccttta acactttttg tgatataatt
     1921 tcttatatag ctgttggatg caactcctcc cgaaataatt aaggttttgt gttga
//
LOCUS       DAOSOW010000354         1965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_57984_length_1965_cov_2.532984, whole genome shotgun sequence.
ACCESSION   DAOSOW010000354 DAOSOW010000000
VERSION     DAOSOW010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57984_length_1965_cov_2.532984"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..980
                     /locus_tag="PLD67_08315"
     CDS             60..980
                     /locus_tag="PLD67_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005951315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOT22566.1"
                     /translation="MTIRHLKIFIEVAQCGNMTAAASKLFISQPTASQAIRELEEHYG
                     VLLFERLSKRLYITEAGKKLLFYAKEVVKEFDSLESKMNNLNKKDKIRVGATISVGDC
                     ILSDLIHRCIEENPNVEIYSFVGNTETIEEKLLSNELDIAIVEGKVKSQDLIVKPELE
                     DYLVLICSTKHPFAQKKIIKTDELRDENFAMREKGSGTRELFEEYMLNNGMPIKVVFE
                     GNTPQSIKQEVIHNNCLSVISICLVENEIKDSLIHVIENEENTWNRHFSLVYHKDKLL
                     TDAIKSLINIVKDYRYIPSKTIVNPGILRK"
     gene            1120..>1965
                     /locus_tag="PLD67_08320"
     CDS             1120..>1965
                     /locus_tag="PLD67_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT22567.1"
                     /translation="MISKKKNLFLILTLILMLITAACSKETNNVEAINIDNQDAEENY
                     YIFSDALGTEVVLERKPERVVSLLGSYSETWALAGGSLAGVTDDVQKEGRMEITNDIQ
                     IVGTVKEPNLEGILSLNPDFVILTPDVDNQVKIAETLKQLNINHAFFKVEEFEDYLNM
                     LNICTDITGRKDLYEENGLKIKKDIDDILYKVEGAKKEELKVLFVRAFSSGAKAKKDD
                     NMTCTMLDNLGTVNIAAQHPSLLEDISIEEIIEEDPDFIFVTTMGDEQKAIDALKTGI
                     GTNPAW"
BASE COUNT          767 a          254 c          383 g          561 t
ORIGIN      
        1 atgataaaat cttatcataa tcataggcaa aacctatgat gttgaaaagg agagttctca
       61 tgactatcag gcatttaaaa atatttattg aagtggcaca atgcggcaat atgactgcag
      121 cagcttcaaa gctttttatt tcacaaccta cggcaagtca agctataaga gaattagaag
      181 agcattacgg ggtattgtta tttgaacggc tcagcaaaag gctgtatatt actgaagcag
      241 gtaaaaaact attgttttat gcaaaagaag tggtaaaaga attcgacagc ttagagagta
      301 agatgaataa tttgaacaag aaagataaaa taagagttgg ggctacaata tctgtcggag
      361 attgtatttt aagcgattta atacacagat gcatagaaga aaatccaaat gtagaaatat
      421 attcttttgt aggaaacact gaaacgatag aggaaaaatt attaagcaat gaattggata
      481 ttgcaatagt agaaggtaaa gttaaaagtc aagacctaat tgttaagcca gaattagaag
      541 attatttagt cttaatatgc agtactaaac acccctttgc tcagaaaaag ataattaaaa
      601 cagacgagct tcgtgatgaa aattttgcaa tgagagagaa aggcagcgga accagggagc
      661 tatttgaaga atacatgctt aataacggaa tgcccataaa ggttgttttt gaaggaaata
      721 cccctcaatc catcaagcag gaagttattc ataacaattg cctgtcagtt atatctattt
      781 gtttggtaga aaatgaaata aaggattctc ttattcatgt tattgaaaat gaagaaaaca
      841 cttggaacag gcacttcagc ctagtatacc ataaagataa attattgact gatgcaataa
      901 agtctctaat aaatattgta aaggattata ggtatatacc ctctaaaaca atagttaatc
      961 cgggtatttt aagaaagtaa ttaatcaata ggttttacct ataatagtga taatatatta
     1021 ttatttttaa ttaaactgct tagatgttat tataaacata aagtatgtgt attaattaac
     1081 aataataata tattttttta taggaaagat ggtggtagca tgataagcaa aaagaaaaat
     1141 ctgtttctta tattaacgct gatattgatg ctgattacag cagcttgcag caaagaaaca
     1201 aacaatgtgg aagcaataaa tatagataat caagatgctg aagaaaatta ttacatattt
     1261 tcagatgcat tgggtacaga ggttgtttta gaaagaaaac ctgaaagagt agtatcttta
     1321 ttggggtcct attcggaaac ctgggctttg gcaggtggaa gcttagcagg tgttaccgat
     1381 gatgttcaga aggaaggacg catggaaata acaaatgaca ttcaaattgt aggtaccgta
     1441 aaggaaccaa atttagaagg aatattatca ctgaaccctg attttgtaat actgactccc
     1501 gatgttgaca accaggtgaa aattgcagaa accttaaaac aattaaatat taatcacgct
     1561 ttttttaaag tggaagagtt tgaagattat ttaaacatgt taaatatatg tactgatata
     1621 acaggcagaa aagacttata tgaagaaaac ggacttaaaa tcaaaaaaga tattgatgat
     1681 atattgtata aggtagaagg tgcaaaaaaa gaagaactta aagttttatt tgttcgtgcc
     1741 ttttcaagcg gagcaaaagc taagaaagat gacaatatga cttgtacaat gctggataat
     1801 ttaggaaccg tcaacattgc agctcagcac ccgtcattat tggaggatat aagtattgaa
     1861 gagattattg aagaagaccc tgatttcata ttcgtaacga ctatgggaga tgaacaaaaa
     1921 gcaatagatg cactaaaaac cggcatagga acaaatccgg cctgg
//
LOCUS       DAOSOW010000355         1956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_58362_length_1956_cov_2.575487, whole genome shotgun sequence.
ACCESSION   DAOSOW010000355 DAOSOW010000000
VERSION     DAOSOW010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58362_length_1956_cov_2.575487"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1956)
                     /locus_tag="PLD67_08325"
     CDS             complement(<1..>1956)
                     /locus_tag="PLD67_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22568.1"
                     /translation="VPNKTFTFAFVPLRVGSEENNSSVSYGGLSPQKEYIRKIFPAPV
                     KFVEKSPMFIAKPRFSTHNMYLSRILRELDRTQKLFSNKYDLLIGMTPRDFLGAAGLQ
                     DSGMFGMNWSFLGAAHGAVLIDPSQVLPHTTLHEIFHTLGLGDVYSEDGVQLQSANGH
                     DGTPINNVPGKSPANEPIMYDHGRTPWPTEGEYNALLEYATKPALGKMSLLGMSTFFE
                     ASQPEVLLLSGSIEDIRSNQRRVDFDTIIKHTGVADESNNYDSNGYVIQTLASDGGVL
                     SQYFFSDYEYEGKYYAKFIANLPAANVQAIEIGKQEYGSITQIFQRYEYSTNAPVVSF
                     TGPATNSLSGDFIITWEASDTDKDALRSEVQVSSDGGNTWNTIASNIPDNTSGNYSYT
                     VNAKAFLKGENYKFKVVVTDGMYSAEDVSGKVYIVEGYEPKPVLNLPNTEATIKVKSG
                     VQAATAYFELSNIGKEELYVELSPDNEASTFVSPLFIKEYTIYPGQKQLIAVPLILPG
                     NAESTLMEVIRLQPKDSDNPTTNLRINVEYTEDDLKPELASVSTNPSDFSGSVNDSPL
                     QVIFEAYAAVGQSSLEATVIIEDNNGIVIADKMAENYHKPGAYTYYWEHEGLPAGNYG
                     VYIGLKDTQSGLERVRTEGEYDLTFNI"
BASE COUNT          541 a          423 c          368 g          624 t
ORIGIN      
        1 ttatattaaa ggttaaatca tattcacctt ccgttctaac cctttccaag cccgattggg
       61 tgtctttcaa tcctatatag acgccatagt tacccgccgg aagaccctca tgctcccaat
      121 agtaggtgta ggcgccgggc ttatgatagt tttcagccat tttatcagca attacaatcc
      181 cattgttatc ttctataata acagtcgctt ctaagctact ttgacctacc gctgcatatg
      241 cttcaaatat aacttgcaga ggtgaatcat ttacacttcc cgaaaaatcg gagggattag
      301 ttgatacaga agctaattcc ggttttaaat catcttctgt atattcaaca tttattctaa
      361 gattggttgt tgggttgtcc gagtcttttg gttgtaaacg aatgacttcc attaaagtgc
      421 tttctgcgtt ccccggaaga attaacggta cagcgattag ttgtttttgg cccggataaa
      481 tggtatattc ctttataaat aaaggtgaaa caaatgttga tgcttcatta tccggtgaaa
      541 gctccacata taactcctct tttccgatgt tgctcaattc aaaatatgct gtagctgctt
      601 gcactcccga ttttaccttt atagttgctt cggtattagg gagattaagt accggctttg
      661 gctcatagcc ttcaacaata tataccttgc ctgatacatc ttcagcagaa tacattccat
      721 ctgttacaac aactttaaat ttatagtttt cacctttaag gaaggctttt gcattcacag
      781 tataagaata attgccgctt gtattatcag gaatattgga agcaatagta ttccatgtat
      841 ttccaccatc agaactaacc tgtacctccg aacgcagagc atccttgtcg gtatcacttg
      901 cctcccatgt aattatgaaa tctccggaaa gggaatttgt agcaggtccg gtaaagctga
      961 ctaccggtgc atttgtggaa tactcgtacc tttgaaatat ttgggttatg ctgccatatt
     1021 cttgtttccc tatttcaatt gcttgcacat tggcggcggg taaattggca ataaatttag
     1081 cataatattt accttcatat tcataatccg agaagaagta ttgtgataac actcctccat
     1141 cagatgcaag ggtttgaatg acatacccat tactatcata attattactt tcatctgcaa
     1201 ctccggtatg ctttattata gtgtcaaaat ctactcttct ttggtttgac ctgatatcct
     1261 cgatactgcc cgacaataac agaacttccg gttgtgatgc ttcaaaaaat gtactcatgc
     1321 caagtaaact cattttccct aaggcaggct ttgttgcgta ttctaaaagg gcattgtatt
     1381 caccctctgt tggccaggga gttcttccat gatcatacat gattggttca ttagccggac
     1441 tttttcccgg aacattattt atgggagtac catcatgacc gttggcagac tgaagctgaa
     1501 ccccgtcttc tgagtaaaca tctcccagac ctaaagtgtg gaaaatttca tgtaaggtag
     1561 tatggggcaa tacctgactg gggtcaatca atactgcacc gtgtgccgct cccaagaaac
     1621 tccagttcat gccaaacata cccgaatcct gcaatccggc tgctcccaaa aagtcacggg
     1681 gtgtcatacc tatgagaaga tcatatttgt tcgaaaaaag cttttgcgtt ctgtccagct
     1741 ctcggagtat cctgcttaaa tacatattat gggtggaaaa acggggtttg gctataaaca
     1801 taggactctt ttccacaaat tttacgggag ccgggaatat cttccttata tattcctttt
     1861 gtggacttag accgccataa gataccgaag agttattttc ctctgaaccc acacgaaggg
     1921 gaacaaaggc aaaggtaaat gtcttattag gtacat
//
LOCUS       DAOSOW010000356         1944 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_58830_length_1944_cov_4.083642, whole genome shotgun sequence.
ACCESSION   DAOSOW010000356 DAOSOW010000000
VERSION     DAOSOW010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58830_length_1944_cov_4.083642"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PLD67_08330"
     CDS             complement(<1..229)
                     /locus_tag="PLD67_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22569.1"
                     /translation="MKKNSLIILPVFIFYIAVFRFIYLENGLSKIIQVCFPIFSGVLL
                     AAILNPVLVFFEKKLKIDNRYISVIMTYLFVF"
     gene            508..1527
                     /locus_tag="PLD67_08335"
     CDS             508..1527
                     /locus_tag="PLD67_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT22570.1"
                     /translation="MKKRLLGNTGMEVSVIGFGGIPIQHVSEKEAINLILECKQQGIN
                     FIDTARVYTVSEKLIGKGLREAGRENFYIASKAMNYTYDGMKSSIEDSLNDLEIDCID
                     LYQVHNVSSKKDLDTVFSENGALRALVEAKEQGLIKHIGITGHIRELLMEAIDNYEFE
                     TLQFPFNPVEPQGTELLLKALKKGMGTIAMKPMAGGTFSYPLLSLKYIMNSGLITVAI
                     PGMDTREQVIENARAGIDDEPLTKEEQEIINKEVEILGTEFCRRCGYCKPCPEGIDIP
                     SVFVFENYVTRYNMPEYGKLKYDTLDVKPDECVRCRKCESRCPYNLPIVEKLKHASET
                     FKNLE"
     gene            complement(1558..>1944)
                     /locus_tag="PLD67_08340"
     CDS             complement(1558..>1944)
                     /locus_tag="PLD67_08340"
                     /inference="COORDINATES: protein motif:HMM:NF025675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HOT22571.1"
                     /translation="LGLWKLYMLIPFVAVAYLIWGLILFAGAERGIKKYLKYPNCIIG
                     KNHKTTLDAKHVHIQIPECKVNTSYPISKLACVFELSTLFLIYISIQDLYILPKRALE
                     DGESDFLRENFRTQLGTRFYTRYDSK"
BASE COUNT          708 a          302 c          373 g          561 t
ORIGIN      
        1 aaaaaacaaa cagataagtc ataataaccg aaatatatct gttatcaatt tttaattttt
       61 tttcaaaaaa aaccaagaca ggatttaaga ttgcagccag aagtacccct gaaaatatgg
      121 gaaagcatac ttggattatc ttagacagac cgttctctaa ataaataaat ctgaaaactg
      181 caatataaaa tataaaaaca ggtaaaataa taagactatt ctttttcaaa ttatcaccta
      241 cacagaaaga agtgtacctt accccctatt attgcgtctt attattcata ttaaaatttt
      301 tcaaaaatta tgtataaata atgaatttct gtaaataatt atgttgataa tacttttttt
      361 atcttcttcc aaattatata aacatcccgc gaaacaaaca ttgcgggatg tcctttttgt
      421 atgccctata actccccttg tctattctcc attgactcaa ataaatttct aatatataat
      481 taacccataa aaacaatgag gtggcatatg aaaaaaagat tgttgggaaa tacgggtatg
      541 gaagtgtctg taatagggtt tggaggaatt ccgatacaac acgtcagtga aaaagaagca
      601 attaacttaa tacttgaatg caagcaacaa gggattaatt ttattgatac ggcaagagtt
      661 tatacagtca gtgaaaaatt aataggtaaa ggtcttaggg aagcaggtcg tgagaatttt
      721 tatattgcct ctaaggcaat gaactatacc tacgatggaa tgaaaagctc aattgaagac
      781 agtttaaatg acttggaaat agactgtata gatttatatc aggttcacaa cgtaagctct
      841 aaaaaggatt tagataccgt tttttccgag aatggagcac tgagagcatt agtagaagcg
      901 aaagagcaag gattaataaa acacatagga ataacaggtc atataagaga acttcttatg
      961 gaagcaattg ataattatga atttgaaact ttacaattcc cttttaatcc cgtagagccg
     1021 cagggaacgg agctattgtt aaaagcattg aaaaaaggga tgggaacaat tgcaatgaag
     1081 ccgatggcag gcggtacatt cagttatcca ttgttatctt tgaaatatat tatgaattca
     1141 ggtcttataa cagtagcaat acccggtatg gacacaagag aacaggttat tgaaaatgca
     1201 agggcaggaa tagatgacga gcctttaaca aaggaagaac aagagataat aaacaaggaa
     1261 gttgaaatat taggaactga attttgcagg cgatgcggtt attgtaagcc ttgtcccgaa
     1321 ggaatagaca tacccagcgt atttgtattt gaaaattacg ttaccagata taatatgcct
     1381 gaatatggca agctaaaata tgatactctg gatgttaaac ccgatgaatg tgtgcgttgc
     1441 cgcaaatgtg agtcaaggtg tccatacaac cttcctatag tagaaaagtt aaaacatgca
     1501 tccgaaacat tcaaaaattt agaataatag tgaggaatta tactatagaa gtacgattta
     1561 tttgctgtca tagcgggtat aaaatcgcgt gccgagctgt gtccggaaat tttcgcgcaa
     1621 gaaatcggat tctccatcct ccaaggcgcg tttcggaaga atatataaat cctgtatgga
     1681 aatatagata agaaacaagg tgcttagctc aaaaacgcag gcaagcttgc tgatggggta
     1741 agaggtgttt accttgcact cgggtatctg tatatgtaca tgttttgcat ctaatgtggt
     1801 tttgtggttt ttgccgatta tacagttggg atatttaaga tattttttta taccgcgctc
     1861 tgctccggca aaaagtatca aaccccatat aagataagcg actgctacaa agggaatcag
     1921 catataaagt ttccaaagtc ccaa
//
LOCUS       DAOSOW010000357         1933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_59333_length_1933_cov_3.493078, whole genome shotgun sequence.
ACCESSION   DAOSOW010000357 DAOSOW010000000
VERSION     DAOSOW010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59333_length_1933_cov_3.493078"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..944
                     /locus_tag="PLD67_08345"
     CDS             <1..944
                     /locus_tag="PLD67_08345"
                     /inference="COORDINATES: protein motif:HMM:NF023591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22572.1"
                     /translation="ILEAVEAIADKNIVKTMSLVDNILNSGKELTFFLDELIICFRNM
                     LIIKTTNSSDNLMRISEDETNEIKELNKKISVEEIVKIIEELSIAQSECKKALNPRVL
                     LETKLIKMLHNVHYVNLDVLVERIENIESSINGKYQPKDAVKEPESTENIERTIKTTS
                     VKKTEKEDILKTEKIENVDKNKEEALENKQQNKSDQAIFDAIISEWQSLLKEIRRENA
                     GLQAIIRESKLTEVINKKAVFEFDSKFTFHINAANQPKNKEEFKEILSRFLNEKCEVE
                     FITKKEIKSVKPKTTIKDVYEDLKNEFGEIVEYKQED"
     gene            946..1296
                     /locus_tag="PLD67_08350"
     CDS             946..1296
                     /locus_tag="PLD67_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOT22573.1"
                     /translation="MAKGRKMPMGMPGGGNMNNMMKQVQKMQRQMEEMQKELEQREVE
                     ATSGGGAVLVKVTGKKNLVELKIDPEVVDKDDVEMLEDMIVAAVNEAFRKADEMMETE
                     MKKVTGGLNIPGLI"
     gene            1296..1892
                     /gene="recR"
                     /locus_tag="PLD67_08355"
     CDS             1296..1892
                     /gene="recR"
                     /locus_tag="PLD67_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOT22574.1"
                     /translation="MEHYTVPITKLIEELAKLPGIGRKSAQRLAFHILEMKSSDVIEL
                     AKAIVNAKKYTKHCQRCGNLTEGELCGICRSLKRDSSTICVVEDARDISAMERTKEYK
                     GVYHVLHGTISPLDGIGPDDINIKSLISRLNTDIKEVILATNPTIEGEATAVYISRLI
                     KPMGIKVTRIAHGIPVGGDIEYADEVTLMRAMEGRREM"
BASE COUNT          805 a          266 c          409 g          453 t
ORIGIN      
        1 ttattttgga agcagttgaa gcaatagcag acaagaatat tgtaaaaacc atgtctttag
       61 ttgacaatat tttaaacagc ggaaaagagc ttacattttt tcttgatgag cttattattt
      121 gttttagaaa tatgcttata attaaaacga caaattcttc cgataattta atgagaatat
      181 ctgaagatga aacaaatgaa attaaagaat taaacaaaaa aatcagtgtt gaagaaatag
      241 ttaagataat agaggagctt tcaatagcac agtctgaatg caagaaggca ttaaatccaa
      301 gggttctttt ggaaacaaag cttataaaga tgctacataa tgtacattat gtaaacctgg
      361 atgtattagt cgaaagaatt gaaaacatag aaagcagcat aaatggaaaa tatcagccta
      421 aagacgcagt aaaagagccg gaaagcacag aaaacattga aagaacaata aagacaacta
      481 gtgtaaaaaa aaccgaaaag gaagatatat taaaaaccga aaaaattgaa aatgtagaca
      541 aaaataaaga agaagctctt gaaaataagc aacaaaacaa aagcgaccaa gcaatttttg
      601 atgccataat tagtgaatgg cagtctttac ttaaagaaat caggagagaa aatgcaggct
      661 tgcaagctat tattagagag tccaagctga ctgaggtaat aaacaaaaaa gctgtatttg
      721 aattcgactc taagtttaca ttccatatta atgctgcaaa tcagcctaaa aacaaagaag
      781 aattcaaaga gatattaagc cgttttttaa atgaaaaatg cgaagttgaa tttattacaa
      841 aaaaagaaat aaaaagtgta aaaccaaaaa caactataaa agatgtatat gaagatttaa
      901 aaaatgagtt tggagaaata gtagaatata aacaggagga ttgaaatggc aaaaggcaga
      961 aaaatgccca tgggtatgcc cggaggcggg aatatgaaca atatgatgaa acaggttcaa
     1021 aaaatgcaaa gacagatgga agaaatgcaa aaagaacttg agcaaagaga agtggaagct
     1081 acatctggcg gaggagctgt cttggttaag gtcaccggca agaaaaattt ggttgaactt
     1141 aaaatagatc cggaagtagt agataaggat gatgttgaaa tgcttgaaga catgattgtg
     1201 gctgcagtta acgaagcttt cagaaaagca gatgaaatga tggaaaccga aatgaaaaag
     1261 gtaacaggcg gattaaacat accggggtta atataatgga gcactatacc gtacctatta
     1321 cgaaacttat agaagaattg gcaaaactac ccggaatagg tagaaaatct gctcaacggc
     1381 tcgcatttca tattttggaa atgaagagca gcgacgttat agagttggca aaagcgatag
     1441 taaatgcaaa aaagtataca aaacattgcc aaaggtgcgg aaatttaacg gaaggcgaac
     1501 tgtgcggcat ttgccgcagt ttaaagaggg acagctcaac catatgtgtt gttgaagatg
     1561 caagagatat ttcagctatg gagcgaacca aggaatacaa aggagtctat catgttcttc
     1621 atggaacaat ttctcctcta gatggaatcg gtcctgatga tataaatatt aaaagtttaa
     1681 tatcaaggct taatacagat ataaaagaag taatcttagc aaccaatccc acaatagagg
     1741 gagaagcaac ggctgtttat atatcccgcc tcataaaacc catgggaata aaggtaacac
     1801 gcatagctca tggaattccg gtaggcggag acatagaata tgccgatgaa gtaactctta
     1861 tgagagcgat ggaaggcaga agagaaatgt aaaaagaatt ccggggacgg acctcaaaat
     1921 tccggtaata ctg
//
LOCUS       DAOSOW010000358         1925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_59691_length_1925_cov_3.085027, whole genome shotgun sequence.
ACCESSION   DAOSOW010000358 DAOSOW010000000
VERSION     DAOSOW010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59691_length_1925_cov_3.085027"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="PLD67_08360"
     CDS             <1..819
                     /locus_tag="PLD67_08360"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOT22575.1"
                     /translation="APYAVPGARWGTRMGNATLLDCMIYDGLWEKFNDYHMGMTAENV
                     AEQYGITREEQDEFAAQSQNRAEEAQKSGKFADEIVPVIIPQKKGDPVVIDTDEYPRH
                     GSTVEKMSKLKPAFKKDGTVTAGNASGINDGAAALIVMSKDKAGELGLKYLCEIAGYG
                     SAGVDPKVMGLGPVPATKKALANAGWTIDDLDLIEANEAFAAQALAVIRDLGLNPEIT
                     NVNGGAIALGHPIGASGARILVTLLYEMEKRDAKRGLATLCIGGGMGTSLLVQR"
     gene            887..1540
                     /locus_tag="PLD67_08365"
     CDS             887..1540
                     /locus_tag="PLD67_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOT22576.1"
                     /translation="MNKIISIDEAANMVKEGMTIMVGGFLANGTPETLIDALVEKNVK
                     NLTLICNDTGFPDKGVGKMVVNKQFKKMIVSHVGTNPETANQMNSGETEVVLTPQGTL
                     AEKVRCGGNGLGGFYTPTGIGTEVAEGKEIRVINGKEYMFETPLRADVALLRASVADK
                     KGNMIFNKTMKNFNPIMATAADLVIVEADKIVEVGEIDPEHVMCSGIFVDYIVGGDK"
     gene            1540..>1925
                     /locus_tag="PLD67_08370"
     CDS             1540..>1925
                     /locus_tag="PLD67_08370"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015392348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HOT22577.1"
                     /translation="MDAKNVIAKRVAELLHDGDVVNLGIGLPTMVANYIPEGMDITFH
                     SENGFLGLGPCPKEGEEDWELVNAGGMPSSIVPGGMFFDSATSFSIIRGGHVDATVLG
                     AMEVDEKGNLANWKIPGKMVPGMGGA"
BASE COUNT          653 a          295 c          489 g          488 t
ORIGIN      
        1 gctccgtatg cggttccggg tgcacgctgg ggaactagga tgggcaatgc gactctgctg
       61 gattgcatga tctatgacgg attgtgggag aagtttaatg attatcacat gggcatgaca
      121 gctgaaaatg ttgcagagca gtatggcatt acaagagaag aacaggatga atttgctgca
      181 caaagtcaaa acagagcaga ggaagctcaa aaatcaggaa aatttgcaga tgaaatagta
      241 cctgttatta tacctcaaaa aaaaggcgac cctgtagtaa ttgatacgga cgaatatcca
      301 aggcatggtt caaccgttga aaaaatgtca aaattgaaac ctgcattcaa aaaggacgga
      361 acagttacgg caggaaatgc atcgggcata aacgacggtg ctgcagcttt aatagtaatg
      421 agcaaggaca aggcaggtga gcttggatta aaatatttgt gtgaaatagc aggttatggc
      481 tcagcaggag ttgacccgaa ggttatgggc ttaggacctg ttcctgcaac caaaaaagca
      541 ttggcaaatg caggatggac aattgatgac ctggatctta ttgaagcaaa tgaagctttt
      601 gctgcacaag cacttgcagt aattagagat ttaggattaa atcccgagat aacaaatgtt
      661 aacggcggag ctatagcatt agggcacccg ataggagcat caggagcaag aatacttgtt
      721 actcttttat atgaaatgga aaaaagagat gccaaaagag gtcttgctac cttgtgcatt
      781 ggcggcggaa tgggtacatc tttattggtt caaagataac aattcggttt gttatttata
      841 gcaatgacaa gttgttgtac tataaaaaga aaggatgata ttattgataa ataaaataat
      901 ttctatagat gaagctgcaa atatggttaa ggaaggcatg acaattatgg ttgggggatt
      961 tttggcaaac ggaactcctg aaaccttaat agatgcattg gtagaaaaaa atgttaaaaa
     1021 ccttaccctt atttgcaacg atacaggttt tcccgataaa ggagtgggaa aaatggttgt
     1081 taataaacag tttaaaaaaa tgattgtgtc acatgtgggg actaatcccg aaacagcgaa
     1141 tcaaatgaat tcgggagaaa cggaagttgt acttactccc caaggaacac ttgctgaaaa
     1201 agttcgatgc ggcggaaacg gtcttggtgg tttttacaca ccaacaggta tcgggactga
     1261 agttgcagaa ggaaaagaaa taagagtaat aaacggcaag gaatatatgt ttgaaactcc
     1321 tttgagagca gatgtggcat tattaagagc ttccgtagct gataaaaaag gtaatatgat
     1381 attcaacaag actatgaaaa attttaaccc gataatggct acagcagcag acttagttat
     1441 cgttgaagcc gataaaatag tggaagttgg ggaaatcgat ccggagcatg ttatgtgttc
     1501 gggtatattt gtagactata tagttggagg tgacaaataa tggatgcaaa gaacgtaata
     1561 gcaaaaagag ttgctgaatt gttacacgac ggtgatgttg ttaacttagg catcggtctt
     1621 cctactatgg ttgcaaatta cataccggaa ggaatggaca taacatttca ttcagaaaac
     1681 ggatttttgg gattaggtcc gtgtcctaag gaaggagagg aagattggga gcttgtaaat
     1741 gcagggggaa tgccttcttc aatagttccc ggcggaatgt tctttgacag cgccacatcc
     1801 tttagcatta tcagaggcgg acacgttgat gcaacagtct tgggagcaat ggaagttgat
     1861 gaaaaaggaa atcttgcaaa ctggaaaata ccgggtaaaa tggttccggg catgggcgga
     1921 gcaat
//
LOCUS       DAOSOW010000359         1923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_59779_length_1923_cov_3.749465, whole genome shotgun sequence.
ACCESSION   DAOSOW010000359 DAOSOW010000000
VERSION     DAOSOW010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59779_length_1923_cov_3.749465"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLD67_08375"
     CDS             <1..196
                     /locus_tag="PLD67_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22578.1"
                     /translation="FIPYCSLKNYRQRLACESYNRPHIQQLKYVSCSAAETESDIGKH
                     AKTACGHFARQTDGIKGYER"
     gene            511..672
                     /locus_tag="PLD67_08380"
     CDS             511..672
                     /locus_tag="PLD67_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007781393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22579.1"
                     /translation="MSQTQITNEIKYKVALSLLNSMLEKGLITLSELKEIDRLNRNSF
                     TPTLAEVYV"
     gene            756..>1923
                     /locus_tag="PLD67_08385"
     CDS             756..>1923
                     /locus_tag="PLD67_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOT22580.1"
                     /translation="MAKVRVIKPIHRMEAEAKAPKLRVCAYCRVSSDHTGQLQSFSAQ
                     VEYYTRLIESNDAWTFAGIYADEGISGTAKDKRDEFLRLIADCEAKKVDMVITKSISR
                     FARNTADCIETVRKLKALGIAVFFEKENINTMSAESELLMTVLSSIAQEESISTSKNN
                     RWAIQKRFMKGEWKPSYRPYGYKKDKDGGIVINEEEAAIVRRIYTDYLNGKGTYVIAK
                     ELTEEGVPTRKGAEAWGENVVKEILMNEKYYGDMLLQKTFTTDTLPFQRKRNRGQKQC
                     YYIANDHEPIISKEQAERVREIMEQRKIEKAMVDTDTQKYNQRYPFSSKIQCGECGSN
                     FKRQIIFKGKPYETVQWCCTKHIMNRAKCSMTAVKDNNIKAAFINLYNKLKSNYD"
BASE COUNT          622 a          422 c          470 g          409 t
ORIGIN      
        1 ttttatccct tattgctctt tgaaaaatta ccggcaaagg ctcgcttgcg agtcttataa
       61 ccggccccat atccaacagc tcaaatacgt tagctgttca gccgctgaaa cggaaagcga
      121 cattggtaaa cacgccaaga cagcctgcgg gcattttgcc cgtcaaaccg atggcatcaa
      181 aggctatgag cgataaatgt ttgaccataa aacaatctgt ggtggattgt ttcgccagga
      241 cctgaacagc ctacaatgat actcctgcat agtcgaggtt gagtcccaga gtgcagcccg
      301 gaggttctcg gggaggtgga attcctgtga tgcctgctga ccacaggcag tttgagctgt
      361 tgcccgggtg cttgggaagc agtggcaaat atagcacagg attaagattg ataacaagct
      421 taaccgcttt gatatcagaa actatgcagc ggaactcaaa tccgctgcat ccttgtggta
      481 ttaaagcaaa atgtaaaggg ggatttaacc atgtcacaaa ctcaaataac caatgaaatc
      541 aagtacaaag tagccctttc gttgctgaac agcatgcttg aaaaagggct tattactctt
      601 tctgaattga aagaaattga cagattaaat cggaattcat tcactccaac tctggcggag
      661 gtatatgtgt aaaaacactt gatatggccg gggttgtgtg gtactgtgtg tagctgacaa
      721 aacatcttgt actgaaaagt gaaaggagga agcgtatggc aaaggtaaga gtcatcaagc
      781 ccatccatcg tatggaagca gaagctaaag ctccgaaact gcgggtgtgc gcatactgcc
      841 gggtcagctc ggaccacacc gggcagcttc aatccttttc agcacaggtg gagtactaca
      901 cccggctgat agaaagcaac gatgcctgga cttttgccgg aatttatgcg gacgaaggca
      961 tcagcggcac tgccaaagac aagcgggatg agtttctaag gctcattgcc gactgtgaag
     1021 ccaaaaaggt ggacatggtc atcaccaagt ctatatccag atttgcaaga aacaccgcag
     1081 actgcattga gacagtgaga aagctgaaag cgctggggat tgccgttttt ttcgagaaag
     1141 aaaacatcaa caccatgtcc gctgaaagcg agctgctgat gacggtttta agctccattg
     1201 cccaggagga atccatttcc acatccaaaa acaaccggtg ggcgatacaa aaaaggttca
     1261 tgaaaggcga atggaagcct tcctaccgtc cctacggata taaaaaggac aaggacggag
     1321 gaatcgtcat caatgaagaa gaagccgcta ttgtccgaag gatttacacc gattacctga
     1381 acgggaaagg aacgtacgtc atcgcaaagg agcttacgga agaaggagtt ccaacaagaa
     1441 aaggggcgga agcctgggga gaaaatgtgg tgaaggaaat actcatgaat gaaaaatatt
     1501 acggcgacat gctccttcag aagaccttta ccaccgacac attgcccttc caaagaaaac
     1561 ggaaccgggg acagaaacaa tgctactaca tcgccaacga ccatgaaccc attatttcaa
     1621 aagagcaggc tgaacgggtc agggagatta tggagcagcg aaaaattgaa aaagccatgg
     1681 tggatacaga cacccagaaa tacaaccaac ggtacccctt cagcagcaaa atccaatgcg
     1741 gtgaatgcgg gagtaatttt aaaaggcaaa tcatattcaa aggcaaacct tatgaaaccg
     1801 tgcagtggtg ctgcaccaag catatcatga atcgggcgaa atgcagcatg acagctgtca
     1861 aggacaataa catcaaagct gcattcatca atctctataa taagctgaaa agcaattatg
     1921 aca
//
LOCUS       DAOSOW010000360         1919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_59961_length_1919_cov_3.446352, whole genome shotgun sequence.
ACCESSION   DAOSOW010000360 DAOSOW010000000
VERSION     DAOSOW010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59961_length_1919_cov_3.446352"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PLD67_08390"
     CDS             complement(<1..477)
                     /locus_tag="PLD67_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOT22581.1"
                     /translation="MKYKYVIFDFDGTLADTEEANFVIYQKLAEKYNLRNITMDELGR
                     LKKMHAKEVMAYIQLKKRYLPFLLKRGKTLLTQDIKNISLCKPDIFHIIGRLRNMGIK
                     TGIITTNSKTNVKLFLETHNVDVFDTIVSAAMFGKEAKMKKIARKAKMSPDKFLYVG"
     gene            complement(575..1153)
                     /locus_tag="PLD67_08395"
     CDS             complement(575..1153)
                     /locus_tag="PLD67_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22582.1"
                     /translation="MPPISTVMHFGKKFMESYDKVDIPNFIIGIILPSTLNDDQLEEL
                     HNVDEDGNIDVREFYEKFDFKTMSLKDSSIVLGYYCHLWLDEYYKFNASKLKIHNKQN
                     LKGEELNSAVKNLLNYYDKKAIGSFYEKYTEDIKAVQSYIFAASNIDNSKKKLLEYLN
                     ETVPDEVITGLIKEEQYMSFIREGCGKIIKSL"
     gene            complement(1319..>1919)
                     /locus_tag="PLD67_08400"
     CDS             complement(1319..>1919)
                     /locus_tag="PLD67_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22583.1"
                     /translation="EDEIKEEVKKVVQEPVKTKKFKEAIKEDSEETMETPEKSSKKDD
                     ISNQGYRNRFDEKLYKTLREFKEIEPLSVKLKNFNWWYIPYDEMGIKTGVLPYYNQII
                     SAYYPYPMSNRVTTCTSLMKKYGHYIFGIYKENDEIARFIYGVPGEFTKEEQPYKGVT
                     GFKNWSYANKDNKGESGYWLAFVNPKTGEITDPPQVSLT"
BASE COUNT          540 a          367 c          245 g          767 t
ORIGIN      
        1 tccaacataa agaaatttat ccggactcat ttttgctttt cttgctattt tcttcatttt
       61 tgcttctttt ccaaacattg cagcactgac aattgtatca aaaacatcaa cattgtgcgt
      121 ttctaaaaaa agcttaacat ttgttttaga attagttgta ataattcctg tttttatgcc
      181 catatttctt agtctgccta taatatgaaa aatatcaggc ttacaaagac ttatattctt
      241 aatatcttga gttaataagg tttttcccct ctttaacaga aaaggcagat atcttttttt
      301 taattgaata tatgccataa cttccttagc atgcatcttt ttcagacggc ctaattcatc
      361 catggtaata tttcttaaat tatatttttc tgctaacttt tgatatataa cgaaatttgc
      421 ttcttcagta tcagctaatg ttccatcaaa atcaaaaata acatatttat atttcactga
      481 cacccatcct taagtatata aattttattt attactatat tatatttttt ttaatcagtc
      541 aatttaaaat taaaaaaact cctttcggag ttttttacaa tgatttaatt attttgccgc
      601 agccttccct gataaaactc atgtactgct cctctttaat aaggcctgtt attacttcat
      661 caggtactgt ttcatttaga tattccaaca actttttctt ggagttatct atattgcttg
      721 ctgcaaatat ataagattgg acagccttta tatcttctgt atatttttca taaaaactgc
      781 cgatagcttt tttgtcataa tagtttaaaa gatttttaac tgcagagttt aattcttcac
      841 cttttaaatt ttgcttatta tgtattttaa gcttgcttgc attaaatttg taatactcat
      901 cgagccaaag atggcaataa taacctaaga caatggagct gtcctttaag ctcatggttt
      961 taaaatcaaa tttctcataa aactccctta catctatatt cccgtcttca tcaacattgt
     1021 gcagctcttc taactggtca tcatttaatg tacttggtaa aattatccct ataataaagt
     1081 taggtatatc aactttatcg taactttcca taaacttttt cccaaagtgc attacggtac
     1141 tgataggcgg catttgtact ttttactcct tttcaattgt taatcgcagc ggggatattc
     1201 tgtcggtaag tccccctcgc tacagggata ttccttcggc catcgaagac tttcccttga
     1261 gtagattgta tccctcttcc tagactatta tatcaaacac aaatcaagat gtacagaatt
     1321 aagttaatga aacttgcggc gggtctgtga tttcacctgt ttttggattt acaaaagcaa
     1381 gccagtatcc gctttctcct ttattgtctt tatttgcata tgaccaattt ttaaatccgg
     1441 tcacaccttt ataaggctgc tcttcctttg taaattctcc gggcaccccg tatataaacc
     1501 gtgcaatctc gtcgttttcc ttgtagatgc cgaaaatata atgcccgtat tttttcatta
     1561 gacttgtgca ggtagtcacg cgattagaca tgggatacgg ataatatgcg cttattattt
     1621 gattgtaata aggcaatact cctgttttta tgcccatttc gtcataaggt atataccacc
     1681 aattaaaatt ctttaattta accgataaag gttcaatttc tttaaattct ctcaatgttt
     1741 tatacaattt ctcatcaaaa cggtttctat aaccctgatt ggaaatatca tcttttttgc
     1801 ttgatttttc cggagtctcc atggtttctt cactatcttc ttttattgct tctttaaatt
     1861 tttttgtctt tacaggttct tgtactactt tttttacttc ttcttttatc tcatcttcc
//
LOCUS       DAOSOW010000361         1908 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_60442_length_1908_cov_2.263896, whole genome shotgun sequence.
ACCESSION   DAOSOW010000361 DAOSOW010000000
VERSION     DAOSOW010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60442_length_1908_cov_2.263896"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="PLD67_08405"
     CDS             <1..805
                     /locus_tag="PLD67_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOT22584.1"
                     /translation="LNNRNHRKIAVIDGKTAYTGGVNLADEYINAVEKFGHWRDSMIE
                     IKGDAVWSLTVMFLSMWGYLRKIDENYRDFRPDDYDMSEECHGYVQPFDDSPLDSEHV
                     GETVYLNLINQAEKYIYIETPYLIIDNKMMVALTMAAKRGVDVRILTPFIPDKWYAFA
                     VTQSNYEDLMDVGVSIYQYTPGFNHGKTFVVDDEYAVVGTINLDFRSLYLHFECGVWM
                     YNTESVGQVKESILEALKISRQVRMEDIKKTARFKKIARTVLRIFAPLM"
     gene            822..1391
                     /locus_tag="PLD67_08410"
     CDS             822..1391
                     /locus_tag="PLD67_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005885201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOT22585.1"
                     /translation="MKKNINFYWKLFLSTFTLSAFTFGGGYVIVPLMRKKFVEELNWI
                     EEEEMLNIVAISQSAPGMIAVNTSIMTGYRLAGVFGAIIATFGTVLPPLIIITIIAGF
                     YTAFKENRIINALLLGMRAGVAAVIIDVIIKMARNVIKTKNNISIAIMVTALIAAVIF
                     DINAALIIILSGVFGGFYYNIVKRTGDSQ"
     gene            1388..>1908
                     /locus_tag="PLD67_08415"
     CDS             1388..>1908
                     /locus_tag="PLD67_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOT22586.1"
                     /translation="MIYLEIFLSFFQIGLFSFGGGYAALPLIEAQVLEYHNWLTIEEF
                     ADLLTISQMTPGPIAINAATFVGTKVAGLPGAVIATIGSVTPSFIIVLTLSYFYFRYK
                     NLSSIQNILKGLRPAVVSLIASAGLSILMLTVFKTEGITLLNMKLSDINFVAIIMVII
                     SVFIFRNLKINPI"
BASE COUNT          650 a          302 c          396 g          560 t
ORIGIN      
        1 cctcaataac agaaaccaca ggaaaatagc ggtaatagac gggaaaaccg cttatactgg
       61 aggagtaaac ctagcggatg aatatattaa tgccgttgaa aaattcggac actggagaga
      121 cagtatgatt gaaataaagg gagatgcagt gtggagttta acggttatgt ttttatccat
      181 gtggggctat ctaaggaaaa tcgatgaaaa ctatcgggac tttagacctg atgattatga
      241 tatgagtgaa gaatgccacg gatacgtcca gcctttcgat gacagcccct tagacagtga
      301 gcatgtcggc gaaaccgtat atttgaattt aatcaaccaa gcggaaaaat acatatacat
      361 agaaacacca tacctcatta tcgataacaa aatgatggta gcattaacaa tggcagcaaa
      421 aagaggagtg gatgtaagaa ttcttactcc cttcataccg gacaaatggt atgcatttgc
      481 tgtaactcaa tctaattatg aggatttgat ggatgtagga gtgtctattt accaatacac
      541 tccgggcttt aaccacggca agacatttgt tgtagatgat gaatacgcgg tggtaggcac
      601 cataaattta gatttcagaa gcctttatct gcattttgaa tgcggagtat ggatgtataa
      661 cacagaaagc gtcgggcagg ttaaagagag tatattggaa gctttaaaaa taagcaggca
      721 agtaagaatg gaagatatta aaaaaacagc tagatttaaa aaaatagcaa ggactgtact
      781 cagaatattt gccccgctga tgtagacaaa cgaggactga tatgaaaaaa aacattaatt
      841 tttactggaa attattttta tcaactttta cattaagcgc ttttactttc ggaggaggat
      901 atgtaatagt acctcttatg aggaagaaat ttgtcgaaga acttaattgg atagaagaag
      961 aagaaatgct gaacatagtt gccatatccc agtctgcacc aggcatgatt gctgtcaaca
     1021 cttcaattat gaccggctac aggcttgcag gagtatttgg agctattata gctacttttg
     1081 gaacagtact gccgccttta atcataataa cgataatagc cggattttat acagctttta
     1141 aagaaaaccg cattataaat gcccttttat tggggatgcg tgccggtgtt gctgccgtaa
     1201 taatcgatgt aataataaag atggcaagaa atgttataaa aaccaagaat aatatttcca
     1261 tagcaattat ggttacagcc ttaattgctg ccgtaatttt tgacataaat gccgctttaa
     1321 taataatact tagcggagtt tttggaggat tctattacaa catagttaaa aggaccggtg
     1381 atagtcaatg atatatctag aaatattctt aagttttttt cagataggat tattcagctt
     1441 tggaggaggt tatgccgctc ttcctttaat agaagctcaa gttttggaat atcataactg
     1501 gcttacaata gaagaatttg cagatttgct gaccatatct cagatgacgc cgggacctat
     1561 tgcaattaat gcagcaacat ttgtgggaac taaggttgcg gggctccccg gagccgtcat
     1621 tgctacaata gggagtgtaa ccccttcttt cattatagta ttaacactgt catatttcta
     1681 ttttagatat aagaatttat catcaattca aaatatactg aagggattaa gaccggcggt
     1741 tgtatcatta attgcttctg cggggctatc cattctaatg ttgacagtat tcaaaacaga
     1801 aggaataacc ctgttgaata tgaaattgag cgatataaat tttgttgcaa taataatggt
     1861 tataataagc gtttttatat ttagaaattt aaaaattaac ccgataaa
//
LOCUS       DAOSOW010000362         1904 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_60597_length_1904_cov_3.060032, whole genome shotgun sequence.
ACCESSION   DAOSOW010000362 DAOSOW010000000
VERSION     DAOSOW010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60597_length_1904_cov_3.060032"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PLD67_08420"
     CDS             complement(<1..444)
                     /locus_tag="PLD67_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008689153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HOT22587.1"
                     /translation="MSVIALAGNQNSGKTTLYNKLTGSNQHVGNFPGVTIEKKEGQLK
                     KYKDISIVDLPGIYSLSPYTMEEVVTRDFLLKEKPDLILNIVDATNIERNLYLTLQLM
                     ELSIPMVIALNMMDEVTASGNSIDVQKLSSHLGIPVVPISASKGEG"
     gene            complement(441..674)
                     /locus_tag="PLD67_08425"
     CDS             complement(441..674)
                     /locus_tag="PLD67_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOT22588.1"
                     /translation="MTLDKIKPGQGGVIASIGGQGLLRRRLLDMGLTPNTCVKVRKVA
                     PMGDPIELSLRSYILTIRKDEAAKIELKEVFDI"
     gene            complement(902..1255)
                     /locus_tag="PLD67_08430"
     CDS             complement(902..1255)
                     /locus_tag="PLD67_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22589.1"
                     /translation="MFALVLILNDIYKLDEINELFYNEELGATSIDSQGIGKVLLDHN
                     INLPILDSVKKLIDGRKPYSKIIFSVIKDREKLDAVIQKLREELDYFEEPGTGFMFVI
                     PVIEVYSHSTENTEF"
     gene            complement(1322..>1904)
                     /locus_tag="PLD67_08435"
     CDS             complement(1322..>1904)
                     /locus_tag="PLD67_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOT22590.1"
                     /translation="SKTSVDDLVNITHIFVTHGHGDHIGDTINIAKRNKALIITNHEI
                     SAYLSKFKLRTHAMHIGGRTKFDFGTVKMTNALHGSGISENGNMIYGGNPGGFIIEVN
                     GKKIYHAGDTGLTYDMKLLEDEKIDVAFLPIGGNYTMDIEDALKAVDFIKPKTVVPMH
                     YNTFGIIKADPEIFKSKASSNVIILNVNESMNV"
BASE COUNT          563 a          396 c          255 g          690 t
ORIGIN      
        1 tccttcacct ttgcttgcag atatgggaac tacaggtatt cccaaatgag aggataattt
       61 ttgtacatct atggaattcc cgctggctgt aacttcgtcc atcatattca gtgcaatgac
      121 cataggaatt gataactcca taagctggag tgtcaagtat aaatttcttt caatatttgt
      181 tgcatcaaca atatttaaaa ttaaatcagg tttctcttta agtaaaaaat ccctggtaac
      241 aacttcttcc attgtatatg gagacaaaga ataaatcccc ggcaaatcca ctatggaaat
      301 atccttgtat ttttttaatt gaccttcttt tttttctatt gtcacaccgg gaaaattacc
      361 gacatgttga ttgcttcctg tcagtttgtt gtataaagta gtttttccgg aattctgatt
      421 tcctgcaaga gcaataacac tcatatgtca aatacctctt tcagctctat ttttgctgct
      481 tcatcctttc gtattgtcaa tatatagctt ctcaaagata attctatggg atcacccatg
      541 ggtgcaacct tcctgacctt cacacaggta ttaggtgtca aacccatgtc aagaagtctt
      601 cttctcaata aaccttgccc gcctattgat gcaatgaccc cgccttgacc cggttttatt
      661 ttatctaatg tcatatcgca cctccaattt cgatattgat aatcattatc atttagatat
      721 tttaattata cccccattga tagcaattgt caacaaaaat taaaaaaaat atgacatata
      781 cggggacctt ccttcaaaaa ttatccgcaa tccatgcgat atctttaata catccacgaa
      841 ttatcggatc ttgaaggtat gctccccttc gataacatca tattcttatt atttattaat
      901 tttaaaattc agtattttcc gtactgtggc tataaacctc tataacaggt attacaaaca
      961 taaagcctgt gcccggttct tcgaaataat ccaattcttc ccttaatttt tgaataacag
     1021 catcaagttt ttctctatct tttattacgc tgaatattat tttactgtat ggttttctgc
     1081 cgtctatcaa cttttttacg ctgtcaagta tcggtaaatt tatattatgg tctaagagaa
     1141 ccttgcctat tccctgactg tctatagaag tagctcctaa ttcttcatta taaaacaatt
     1201 catttatttc atcaagctta taaatatcat ttaatatcaa cactaaagca aacatataac
     1261 ctccctggtt ctgatgatat tgttattata ccatatgagt tttctgctat tcaacaatca
     1321 tttacacatt catgctttca ttaacattca atattattac attcgatgat gctttactct
     1381 taaatatttc agggtcagct ttaatgatgc caaatgtgtt ataatgcatt ggaaccacgg
     1441 tctttggttt tataaaatct actgccttaa gggcatcctc aatatccatg gtataattgc
     1501 cacctatagg cagaaaggca acatctattt tttcatcctc caaaagtttc atatcatatg
     1561 taagccctgt atcaccagca tggtaaatct ttttaccgtt tacctcaata ataaatcctc
     1621 ccggattgcc tccgtaaatc atatttccgt tttcggaaat accggagccg tgcagagcat
     1681 ttgtcatttt taccgttcca aaatcaaact ttgtcctgcc gccgatatgc attgcatgag
     1741 ttcttaattt aaacttgctc aaataagcag aaatttcgtg attagtaatt atcaatgctt
     1801 tatttctttt cgctatatta attgtatctc ctatatgatc accatgccca tgagttacaa
     1861 atatatgggt tatattcact aaatcatcaa cacttgtttt actc
//
LOCUS       DAOSOW010000363         1901 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_60742_length_1901_cov_3.144095, whole genome shotgun sequence.
ACCESSION   DAOSOW010000363 DAOSOW010000000
VERSION     DAOSOW010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60742_length_1901_cov_3.144095"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="PLD67_08440"
     CDS             complement(<1..982)
                     /locus_tag="PLD67_08440"
                     /inference="COORDINATES: protein motif:HMM:NF018851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOT22591.1"
                     /translation="MDNNLDSVLDMYLFETNSLLEQLDNILLKAESNLSFDKECMDEI
                     FRAMHTIKGSYAMMQFDSIMTISHKIEDLFYYIRENGIDQKYNEELVNLVFKITDFIK
                     GEIVKVEEGLELEKNLDPLENEIKLFLNSIKKETKEEIYQIENLKSNIAITEENNQTK
                     GCTYAIRIIFDDDVSMENLRAFIIVNQLRETGIEFEYKPENIENIPETSQEIIENGFF
                     VYVNKEYLDTATEVISKALNVKNYTVFEYNEEVQNNKEDETIINQTRCSTDLNNSNLS
                     ANSKQCRQSLITVNLSKLDKLVDIVGELVIAESMVESDADIKRLNSESFKK"
     gene            1208..>1901
                     /locus_tag="PLD67_08445"
     CDS             1208..>1901
                     /locus_tag="PLD67_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22592.1"
                     /translation="MNNISVKTNNHISENPINKPKIKNEVPFDIADPTKVTKTPEKGY
                     ENQIRQELFNYNPDSVFDKFIKSLQNSPVLYEEAKKLLLSKQFINNNIKNDPVLSVFF
                     DSFLGNIKMNDAEILDFLKFQQGTYTKFQGEFFDLLRTLLGNNPDNKGLQTVLKNFLR
                     SYDCFVSVKEIYGSINATFKNIEKNIPEMLKASFNELTGKFIYDNTNAMDSNLELLKN
                     EILPFIGRYISKM"
BASE COUNT          610 a          305 c          262 g          724 t
ORIGIN      
        1 attttttaaa gctttcagaa tttaatcttt ttatatctgc atccgactca accatggatt
       61 cagcaataac aagctctcca acaatatcta caagtttgtc aagctttgaa agattgactg
      121 ttattaagct ttgcctgcat tgttttgaat ttgccgataa atttgaattg tttaaatcag
      181 tggagcatct tgtttggttg ataattgttt catcttcttt attattttgt acttcctcat
      241 tgtattcaaa tacagtataa tttttgacgt ttaatgcttt actgattact tctgttgccg
      301 tatccagata ttccttattc acatatacga aaaagccgtt ttcaattatt tcttgcgatg
      361 tttcgggaat gttttcgata ttttcaggtt tatattcaaa ttcaatacct gtttccctca
      421 attgattgac tataataaat gctcttaaat tttccatgct tacatcgtca tcaaatatta
      481 ttcttatggc gtatgtgcat ccctttgttt ggttattttc ttcagtaatt gcaatatttg
      541 attttagatt ttctatttgg tatatttctt ctttggtttc ttttttgatt gagtttaaaa
      601 ataactttat ttcattttct aaagggtcta aatttttctc aagctcaagc ccttcttcta
      661 ccttgacaat ttctcctttt ataaaatcag taattttaaa aactaaattt acaagttctt
      721 cgttatactt ttggtctatt ccattttctc tgatataata aaacaaatct tcaattttgt
      781 gagaaatagt cattatactg tcaaattgca tcatggcgta tgagcccttt atggtatgca
      841 tggctctgaa tatttcgtcc atgcattcct tatcaaaact taaattgctt tcagccttta
      901 acaaaatatt atctaattgt tccaataatg aatttgtttc aaaaagatac atatcaagaa
      961 cagaatctaa attattatcc atatttatct ccttgttggt ttcctatctt tatatcgtcc
     1021 tttattatga attgttaagc tattataacg aaaaactttt tataaatttt tcgataatac
     1081 tcttatttaa tttattatcg tgccgatata caagatgagg ggggacacac ctttaccata
     1141 tggttagtct ttggagcata aggaatgtcc cccaattggt attaacttta taaaacagga
     1201 gattattttg aataatataa gtgtcaagac taataatcat attagcgaaa atcccattaa
     1261 taagcctaaa attaaaaacg aagtgccttt tgatatagct gatcctacta aggttacaaa
     1321 gacgcctgag aagggttatg aaaatcaaat cagacaagaa cttttcaatt ataatcctga
     1381 ttccgttttt gacaagttta ttaaatcatt gcaaaattct cctgtattat atgaagaagc
     1441 taagaagctc cttttaagca aacagtttat caacaacaat attaaaaacg acccggtgtt
     1501 aagtgtattt tttgattctt ttcttggcaa tattaaaatg aatgatgctg aaatattgga
     1561 ctttcttaag ttccagcaag gaacatacac taaatttcaa ggcgaatttt tcgatttact
     1621 gagaactttg ttaggcaata atcctgacaa caaaggactt caaacggttt taaaaaattt
     1681 cttaagaagc tatgactgct ttgtatcagt gaaagaaata tacggctcaa ttaacgcaac
     1741 ctttaaaaat atcgaaaaaa acattccgga aatgttaaaa gcttctttta atgagctaac
     1801 gggaaaattt atttacgata atacaaatgc catggactca aacttggagc tgttgaaaaa
     1861 tgaaattctg cccttcattg gaagatatat ttccaaaatg a
//
LOCUS       DAOSOW010000364         1894 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_61087_length_1894_cov_2.871669, whole genome shotgun sequence.
ACCESSION   DAOSOW010000364 DAOSOW010000000
VERSION     DAOSOW010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61087_length_1894_cov_2.871669"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PLD67_08450"
     CDS             complement(<1..285)
                     /locus_tag="PLD67_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOT22593.1"
                     /translation="MAKATREAYGEALKKLAASNPDIVVLDADLSGSTKTSEFKKVSP
                     ERFFNVGIAEQNLIGTAAGLSLAGKIPFASSFAMFAAGRAFEIIRNTVAYP"
     gene            complement(278..1120)
                     /locus_tag="PLD67_08455"
     CDS             complement(278..1120)
                     /locus_tag="PLD67_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018498965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOT22594.1"
                     /translation="MKYDELKVIANSMRKNILTMIYKAKSGHPGGSLSAVEIMVYLYY
                     KEMKIDSPIDENRDRFVLSKGHGAPVLYAVLMEKGFISKDLIPTLRQIDSKLQGHPDM
                     KKLPGVEASTGSLGQGLSIANGMALAFKLDKKPNRVFALLGDGEINEGMIWEAAMLAS
                     HYNLDNLTAVLDHNGLQIDGKNEDVMTIEPIDKKFEAFGWHVIKTEGHSFESLEKAFE
                     ERKTIKGKPAIIIANTIKGKGCSFMENQAGWHGKAPNEGEFCKAMEEMGDIGDKTEGG
                     GYNG"
     gene            complement(1135..1779)
                     /gene="fsa"
                     /locus_tag="PLD67_08460"
     CDS             complement(1135..1779)
                     /gene="fsa"
                     /locus_tag="PLD67_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013430193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HOT22595.1"
                     /translation="MKLFIDTANVEEIRKANDMGVICGVTTNPSLIAKEGRIFKDVVK
                     EITDIVDGPISAEVISLDSEGMIKEAVELSKIHKNIVIKIPMTAEGLKAVKVLSKEKI
                     KTNVTLIFSAGQALLAAKAGASYVSPFVGRLDDIGNDGMNIIKETVEIFKNYNIDTEI
                     IAASIRHPMHVIEAAKLGCHIATIPYKVIMQLTKHPLTDKGIDQFLKDWDTVPK"
BASE COUNT          480 a          438 c          285 g          691 t
ORIGIN      
        1 aggatatgca acagtatttc taataatttc gaatgccctt cctgcagcaa acattgcaaa
       61 ggagcttgca aacggaattt tacctgccaa tgacagccct gctgccgtac cgatcaaatt
      121 ctgctctgca attcccacat tgaaaaatct ttcgggactt acctttttaa attccgatgt
      181 ctttgtggag ccggataaat ccgcatccaa tacgactata tcaggatttg atgctgctaa
      241 tttttttaaa gcttcaccgt atgcttctct ggttgcctta gccattatat ccgcctcctt
      301 ccgttttgtc tccgatgtct cccatttcct ccattgcttt gcaaaactcg ccttcattcg
      361 gtgcctttcc atgccagcct gcctggtttt ccatgaaaga gcaacccttc cccttgatag
      421 tatttgcaat tattattgcc ggtttccctt ttattgtttt tctttcttca aaagcttttt
      481 ccaaagattc gaaactatgc ccttctgttt ttatcacatg ccatccgaat gcttcaaatt
      541 ttttgtcaat cggctcaatt gtcataacat cttcattttt gccatctatc tgcaaaccgt
      601 tatggtccaa tacggcggtt aaattatcaa gattgtagtg gcttgcaagc attgctgctt
      661 cccaaatcat accctcgttt atctcaccgt ctccaagaag tgcaaatacc ctgtttggct
      721 ttttgtctag tttaaatgcc agagccattc cgtttgcaat agaaagtccc tgccctaagg
      781 agccggttga agcttctacc ccgggaagtt tcttcatatc gggatgacct tgaagcttgc
      841 tgtcaatctg tcttagagtg ggaattaaat ccttgcttat aaagcctttt tccatcaata
      901 cggcatacaa aacgggagct ccatgtccct ttgataatac aaatctgtct ctgttttcat
      961 ctattggaga atctattttc atttccttgt agtacaaata caccattatt tccaccgcag
     1021 aaagagagcc tcccggatgg cctgacttag ccttataaat catagtcaat atattttttc
     1081 tcatactatt ggctatgact tttaactcat cgtatttcat tttttcctcc aagtttattt
     1141 aggtaccgta tcccagtctt tcaaaaattg gtctattccc ttatcagtca aaggatgttt
     1201 tgtcagttgc attattacct tataaggaat ggttgcaata tggcatccta actttgcagc
     1261 ctcaataaca tgcatcggat gtcttatgct tgcagctata atttcggtat caatattata
     1321 atttttaaaa atttcaactg tttctttaat aatattcatt ccgtcattac ctatatcatc
     1381 aagccttccc acaaaaggac tgacatagct tgcgcctgct tttgcggcta gaagtgcctg
     1441 acctgcagaa aatatcaagg ttacattggt ttttattttt tccttgctca aaacctttac
     1501 tgcctttaac ccttcagcag tcataggaat tttaataact atatttttat gtattttaga
     1561 aagttctaca gcttctttta tcattccttc cgagtccaag ctgataactt cagcacttat
     1621 cgggccgtca actatatcgg ttatttcttt taccacatcc ttaaatattc ttccttcctt
     1681 tgctattaat gacgggtttg tggtaactcc gcatataact cccatatcat tagcttttct
     1741 tatttcttca acattagccg tatcgataaa taatttcatt ttttcctcct attttattat
     1801 ttacatcatt tttatcaata acccacagag tgtcattgtt atgaataaca tttttgtcat
     1861 tctgagagct ttagctcgaa gaatctcatg agat
//
LOCUS       DAOSOW010000365         1893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_61125_length_1893_cov_3.650163, whole genome shotgun sequence.
ACCESSION   DAOSOW010000365 DAOSOW010000000
VERSION     DAOSOW010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61125_length_1893_cov_3.650163"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PLD67_08465"
     CDS             complement(<1..384)
                     /locus_tag="PLD67_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOT22596.1"
                     /translation="MAVGTLNVEKRLETNSRASNRLRRQGYLPGNISRKGKDSVSVTV
                     KADELRKGLSTYGRNALFKITLDDTEFTGMVKDIQLSPVKGSMMHVDFQEVSLNEEIK
                     VQLSIVLKGTEALEFKGLIALTQLDS"
     gene            complement(506..1567)
                     /gene="ispG"
                     /locus_tag="PLD67_08470"
                     /gene_synonym="gcpE"
     CDS             complement(506..1567)
                     /gene="ispG"
                     /locus_tag="PLD67_08470"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOT22597.1"
                     /translation="MDKIIRRQSSKVMVGNVQIGDGADITVQSMTNTFTKDVKSTVAQ
                     ILQLEEAGCEIIRVAVADNEDAEAIKEIKKQIHIPIVADIHFDYRLALKSIESGINKL
                     RINPGNIGGIDRVKKVVEKAKPRNIPIRIGVNVGSIHKEILKKYNAPTSEGMVESALE
                     HVRILEDLDYGNIVISMKGTDIKMTIDAYKSMAKKVNYPLHLGITHAGTLFEGSIRSA
                     IGVGSLLADGIGDTIRISLTDDPREEVKVAREILKSLNLRNFGINYITCPTCGRTKIQ
                     LIELSKKIQEGLKNVNKPITVAIMGCAVNGPGEARQADIGIAGGKGEALLFKKGEIIE
                     KVKEEDLVKRLLEEIDKLS"
     gene            complement(1692..>1893)
                     /locus_tag="PLD67_08475"
     CDS             complement(1692..>1893)
                     /locus_tag="PLD67_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOT22598.1"
                     /translation="INLGFMNLLPFPALDGSKLVFLAIEGIRGKALPVEKEGYIHFIG
                     FVFLIGFMLFITYKDLLRVFRI"
BASE COUNT          522 a          409 c          258 g          704 t
ORIGIN      
        1 tgaatctagt tgtgttaatg ctattaatcc tttaaattct aatgcttctg tacctttaag
       61 cacaattgat aattgaactt tgatttcttc atttaatgat acttcttgaa agtcaacatg
      121 catcattgag cccttaacag gtgaaagttg aatatctttc accataccgg tgaattccgt
      181 gtcatccaat gttattttaa acaaagcatt ccttccgtaa gttgataagc cttttctcag
      241 ttcatcagct ttaacggtaa ctgaaaccga atccttacct tttctggata tgtttccggg
      301 caaatatcct tgtcttcgca atctgttgct tgctctgctg tttgtttcta atctcttttc
      361 tacatttaaa gttcctactg ccatgtttcc tccttagata tgcaatttat ttaatacgct
      421 ttggtttata cagcgataag gttaattaaa aaatattgaa ttttcatata taaattatac
      481 tacacctttt gtttgaatgt caaccttagg agagtttatc aatttcttcc agcagccttt
      541 taactaaatc ttcttctttt accttttcta ttatttcccc ctttttaaac aacagggcct
      601 ctcctttacc tcctgctata ccgatatccg cctgtcttgc ttctcccggt ccgttaactg
      661 cgcagcccat tattgcaact gttataggtt tattaacatt ctttaaacct tcctgaattt
      721 ttttggatag ctcaataagc tggattttag ttcgaccgca tgttggacat gttatataat
      781 ttattccgaa atttcttaaa ttaagacttt ttaaaatttc tctagcaacc tttacttctt
      841 ccctcgggtc atctgttaga gatattctta ttgtgtcgcc aataccatca gccaaaagac
      901 ttccaacacc aatggcggag cgtatgctgc cttcaaataa cgttcctgca tgagtaattc
      961 caagatgaag aggataatta acctttttcg ccatagattt gtaggcgtca atggtcattt
     1021 ttatatctgt tcccttcata gaaattacaa tgttgccgta atcaaggtcc tctaatatac
     1081 gaacatgctc caaagcagat tcaaccattc cttctgaagt gggagcattg tactttttta
     1141 atatttcctt atgtatactt cctacattta cgcctattct gattggaata tttcttggtt
     1201 ttgccttttc tacaaccttt ttaaccctgt caatgccgcc tatattaccg ggattaattc
     1261 gcaatttatt aattccgctc tcaatgcttt ttaatgctaa tctatagtcg aaatgtatat
     1321 cagcaacaat cggtatatga atttgttttt ttatttcttt aattgcttca gcatcttcat
     1381 tatcggcaac cgccaccctt ataatttcgc agcctgcttc ttctagctgc agaatttgag
     1441 caaccgttga ctttacatct ttggtgaaag tattagtcat ggattgtacc gttatatctg
     1501 caccgtcacc tatttgtacg ttaccgacca taacttttga gctttgtctt ctgattattt
     1561 tatccatact tttctatcct ctccttataa aagatgatat tcttcactgc tttcagaata
     1621 acaaaatgtt tatgaataca aaacaggtgt gtcaaatctc aataccagat gacgacaccc
     1681 ccgtcccctt gtcatattct aaatactctc aataaatcct tataagttat aaacaacata
     1741 aaacctatta aaaaaacaaa acctataaag tgtatataac cttctttttc aacaggcaat
     1801 gctttacctc ttattccttc tattgccaga aataccagct tgcttccgtc caatgccggg
     1861 aaaggaagta aattcataaa gcccaaatta atg
//
LOCUS       DAOSOW010000366         1893 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_61142_length_1893_cov_2.491839, whole genome shotgun sequence.
ACCESSION   DAOSOW010000366 DAOSOW010000000
VERSION     DAOSOW010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61142_length_1893_cov_2.491839"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..1159
                     /gene="flhB"
                     /locus_tag="PLD67_08480"
     CDS             98..1159
                     /gene="flhB"
                     /locus_tag="PLD67_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HOT22599.1"
                     /translation="MAGENKTEKASPKKRRDERKKGNVFQSKDVVTVGVIAISFLILN
                     LWAPYIYKYTTNVFYKYVILTKNTYTINDNLIAQAFRDITFTIFASAGLVMFFVITTT
                     VVLTGAQTKFLVSKENIKFKFSKLNPLSGIKRMFSIRSLVEVIKNLIKISILAYVIYS
                     SIANNLFQVPRLISVNLAAGINFIRTSVMSIVKSVVIAFIVISAFDFLYSWWEYEKSI
                     RMTKQEVKEEYKQLEGDPKIRGIIKEKQRAFSMTRMMQQVPKADVIIRNPTHYAVAIS
                     YNIEKDNAPLVLAKGKDRMAARIIEKAEEYKINIIENIPLARALYAESEINREIPVKY
                     YEDVAEILAWVYRLKEKGI"
     gene            1163..>1893
                     /locus_tag="PLD67_08485"
     CDS             1163..>1893
                     /locus_tag="PLD67_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOT22600.1"
                     /translation="MRSIFKNSISIFIIAVILMIIVPLPPFFLDLMFIINIAFAIVIL
                     LTTMYVREALEFSVFPSLLLITTLFRLSLNVSSTRLILTNNGHAGAVIATFGSFVLQG
                     NAVVGFIIFLIIVLVQFIVITKGAERVAEVTARFTLDAMPGKQMAIDADLSSGIITEQ
                     EAMQRRKGVQRESDFYGAMDGATKFVKGDAIMSIVITFINFVGGVIIGMVQGGDTFAG
                     VLQTYSIATVGDGLVSQIPALLISTA"
BASE COUNT          667 a          288 c          362 g          576 t
ORIGIN      
        1 tatacctaca ttttcaacat ttatagaaaa tgtaatcgta ttgatgtttg atactgtata
       61 aaacggtcta agcgcattaa ttacataagg tgaagtgatg gcaggcgaaa ataaaaccga
      121 aaaagcttcc cccaaaaaaa gacgggatga aagaaaaaaa ggtaatgtat ttcagagcaa
      181 ggatgtagta accgttggag taattgcaat atcatttttg atattgaatc tatgggcccc
      241 atatatttat aagtatacta caaatgtatt ttataaatac gtgatcctga caaaaaatac
      301 ttatacaata aatgataatc ttattgctca agcgttcagg gatattacct ttaccatatt
      361 tgcatcagcg ggactcgtaa tgttttttgt aataacaacg actgttgtat taaccggtgc
      421 acaaactaag tttttagttt ctaaggaaaa tataaaattt aagttttcaa aattaaatcc
      481 gctttccggc ataaaaagga tgttttctat ccgttctctt gtggaggtaa taaagaattt
      541 aattaaaata tcaatattag catatgtaat atattcttcg attgcaaaca atttatttca
      601 ggtacccaga ttgatttcag tcaatctagc tgccggaata aatttcataa gaacatcggt
      661 tatgtccata gtaaaaagtg ttgtaattgc ttttatagtt atttctgcat tcgacttttt
      721 atattcctgg tgggaatatg aaaaaagtat cagaatgacc aagcaggaag ttaaggaaga
      781 atataaacaa ttagagggag accctaaaat aagaggaata ataaaagaaa aacaaagagc
      841 cttttcaatg acaagaatga tgcagcaagt accaaaagcc gatgttatta taagaaaccc
      901 tacacattat gctgttgcta taagttataa cattgaaaaa gacaatgctc ccctagtact
      961 ggcaaaagga aaagacagaa tggcagcaag gattattgag aaggcagagg aatataaaat
     1021 taacattatt gaaaacattc cattggcaag ggctctttat gccgagtcag aaataaatcg
     1081 ggaaataccc gtaaaatatt atgaagatgt tgcagaaatt ttagcttggg tttataggct
     1141 taaggaaaaa ggaatataag atatgagaag tatattcaag aattctattt caatatttat
     1201 tatagcagta atacttatga taatagtacc attacctccc ttctttttgg atttaatgtt
     1261 tattatcaat attgcatttg ccatagttat attgctcaca acgatgtacg taagggaagc
     1321 attggaattt tccgtatttc cttcgcttct tttaataact accctattca gactgtcctt
     1381 aaatgtatct tccacaaggt tgatcttaac aaacaatgga catgcagggg cagttatagc
     1441 cacattcgga agctttgtat tacaaggaaa tgcagttgtc gggtttatta tatttttaat
     1501 tatagtcttg gttcaattta tagtaattac aaagggcgcc gaaagagtag ccgaagttac
     1561 cgcaagattt accttggatg caatgcccgg aaaacaaatg gcaattgatg cggatttaag
     1621 ttcaggcata atcacggagc aggaggcaat gcaaagacgt aaaggcgtac aaagggaatc
     1681 ggatttttac ggagcaatgg atggtgcaac aaaatttgta aagggtgacg ccataatgtc
     1741 aatagtaatt acctttataa attttgtagg cggtgtcatc ataggtatgg ttcagggagg
     1801 cgacactttc gccggagtac ttcagactta ttccatagca accgtaggtg acggactggt
     1861 ttctcagata cctgctctac taatatctac ggc
//
LOCUS       DAOSOW010000367         1891 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_61239_length_1891_cov_2.394336, whole genome shotgun sequence.
ACCESSION   DAOSOW010000367 DAOSOW010000000
VERSION     DAOSOW010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61239_length_1891_cov_2.394336"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="PLD67_08490"
     CDS             <1..1483
                     /locus_tag="PLD67_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22601.1"
                     /translation="RALAADLAEATEQSKNMNNFIQYLTDFANDLSGKTNPADRFIQK
                     IIPGGRMTFRAINWLNNRVKANTVLGNLSSALSQIANVPQGIAYVKNPAHLTKGAGNF
                     MASIFGKGDADLYKKSGFLLERYSDNIARQFDVKILEQPKKAAIWLMNALDEVGTKFI
                     WSSTYQKALAEGVQNPIKYADDITRKLVAGRGIGEVPLLQKSKLFQLVAPFTVEVNNL
                     WKVQKDFVKAKDFGGLALLYLANFALNKGMEEVRGDGVVFDPIQAIYDATEKDLKPIE
                     RVGRLGGEVLSNMPLGSTVASLYPEYGISNDFIDLPTREKLFGRTDPTRYGSGLLVAK
                     GLQDPLYKVLPPFAGGQAKKTIQAGQDLGLLPKLKKEEGKLLPTLKKQDLPASYSKTK
                     AGDRLRTTINPKASTTAKGLMFGRGAIPEMKAYYESGKVPFGTKQTENFHKLVDKGYD
                     PNKLFTTLNSIRGTSKKKDIVVKLREYGYNDREINEIWEIFYK"
BASE COUNT          673 a          333 c          411 g          474 t
ORIGIN      
        1 cagagcattg gcagcagatt tagcagaagc aacggaacaa agcaaaaaca tgaataactt
       61 tatacagtat ttaaccgatt tcgctaacga cctcagtggc aagactaacc cggccgatag
      121 attcatacaa aaaataatac ctggcggcag aatgacattc agggcaataa actggttaaa
      181 taatagagta aaggctaata ctgttttagg taatctatct tcagcacttt cacagattgc
      241 caatgtaccg cagggaatag cctatgtcaa aaacccggct cacttaacaa aaggagcagg
      301 aaactttatg gcttcgatat tcggcaaagg tgatgctgac ctatacaaaa agtcagggtt
      361 ccttctggaa agatactccg acaacatagc aagacagttt gatgtaaaaa tacttgaaca
      421 acctaaaaag gctgctatat ggttgatgaa cgcattagat gaagtcggaa caaaatttat
      481 atggagttct acataccaaa aggcattggc agaaggagtg caaaacccga taaagtatgc
      541 tgatgatatt acgagaaaac ttgtagctgg ccgaggaatc ggtgaagtac cattactgca
      601 aaagtcaaaa ctgtttcaat tagtcgcacc tttcaccgta gaggttaata atttatggaa
      661 ggtacaaaaa gactttgtaa aagccaaaga ctttggcggc cttgcattgt tgtatctggc
      721 taatttcgca cttaataaag gcatggaaga agttagaggg gacggagttg tatttgaccc
      781 gatacaggca atctatgacg ctacagaaaa agacctgaag cctattgaaa gagttggaag
      841 gttaggcggt gaagttcttt ctaatatgcc gttaggttca acagtagctt cattatatcc
      901 tgagtatgga atcagtaatg actttataga tttgccgaca agggaaaagc tattcggcag
      961 aacagaccct acaaggtacg gttcgggctt actggtggca aaaggcttgc aagacccatt
     1021 atacaaggta ttaccgccat tcgcaggagg acaggctaaa aagaccattc aagcagggca
     1081 agacctggga ttgttgccta agttgaaaaa ggaagaagga aaactacttc ctacgcttaa
     1141 aaagcaggat ttacctgctt catactccaa aaccaaagca ggtgacaggc tgagaacaac
     1201 cataaatcct aaagcttcaa ccacagcaaa aggattaatg ttcggacgtg gagcaattcc
     1261 tgagatgaaa gcatattatg aatcaggcaa agttccattt ggcacaaaac agacagagaa
     1321 tttccataag ttagttgata aaggatatga ccccaataaa ctattcacaa cacttaactc
     1381 tatcagagga acctcgaaga aaaaagatat tgttgtaaaa ttaagggaat acggttataa
     1441 tgatagagaa ataaatgaaa tatgggaaat cttttataag taggtggtag tgtggaatat
     1501 atcatgtatg gcgattaccc aagtgggtta ataattgtag caattgtaat atttgtatgt
     1561 tggataatta cgaaagtgaa ggatataaaa tggccgtcgc agaagttcta attacaatag
     1621 ccttagccat actatcatat atactctatc catatttaca tgatcttatt tataagagac
     1681 gataattcgt ctctttttat tttgggatta accggcaggg aaccggaaca gcatacccct
     1741 gccggagccg caaacaaggc ggctattttt attatacgcc tttgcggaat taaagcaaac
     1801 aaggaggaaa gaaaaaatga gtaaacaatt agcaatattt aacaccgtaa tagctgctat
     1861 aggggcaacg attacggcag cattaggtgg t
//
LOCUS       DAOSOW010000368         1888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_61349_length_1888_cov_3.164757, whole genome shotgun sequence.
ACCESSION   DAOSOW010000368 DAOSOW010000000
VERSION     DAOSOW010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61349_length_1888_cov_3.164757"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLD67_08495"
     CDS             <1..275
                     /locus_tag="PLD67_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOT22602.1"
                     /translation="WFLGTGYYGTELLKYIIEFFISIASLGFIIGLCYHYCKNLFIPI
                     WMHFVLNFLSQTCTGSQLDMVTWYAVFYLITAIGFALYHKLRLRKG"
     gene            526..1161
                     /locus_tag="PLD67_08500"
     CDS             526..1161
                     /locus_tag="PLD67_08500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOT22603.1"
                     /translation="MIKIRREEQTDYERVEEITRKAFWNLYTPGCNEHYLVHVMRSHK
                     DFLPELDFVIEVDNQIIGNIMYTKARLIDEAGEEKNILTFGPVSILPEYQRRGYGKKL
                     LEYSFEQAAALGYDVIVIFGNPGNYVSRGFKSCKKFNVCLEDGTYPTAMLVKELKPNV
                     LDGRKWVYYQSPVYEIDEQEALCFDDALEKMEKKYLPCQEEFYILSNSIIK"
     gene            1194..1550
                     /locus_tag="PLD67_08505"
     CDS             1194..1550
                     /locus_tag="PLD67_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1224..1226,aa:Sec)
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HOT22604.1"
                     /translation="MKYTFICYSRUGTCKKAKDWLIKNDIDFTERSIKEENPNESELI
                     EWIKKGKCSIKRFFNTSGTLYKDLNLKEKLENMSDEEQIKLLSTYGMLVKRPILVGGD
                     IVLVGFKENEWREKLL"
     gene            1599..>1888
                     /locus_tag="PLD67_08510"
     CDS             1599..>1888
                     /locus_tag="PLD67_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22605.1"
                     /translation="MNIKYIKELLSRINEVRIINKKADIEGVVCNIAGIVRYGQKLRL
                     IILEYDEVYRQKVEEMELSEPCELRQQETNRSILMGRGRIEAAQPFRPIKSV"
BASE COUNT          681 a          231 c          406 g          570 t
ORIGIN      
        1 tttggttttt aggtacaggg tattatggaa ctgaattatt aaaatacata atagaatttt
       61 ttattagtat tgcatctctt ggttttatca taggtttgtg ctaccattac tgcaaaaatt
      121 tgtttattcc tatatggatg cattttgttt taaatttctt atcacaaaca tgcacaggct
      181 cgcaattgga tatggttaca tggtatgctg tattttattt aattacagca ataggttttg
      241 ctttgtatca taagttaagg ttaagaaaag gataatgaat ggaagtttat agaaaatatt
      301 ttgccaaaga aggctgttta tgtggtatta tatacatgtc accagctgtg aaaaagaaaa
      361 tttaagatat tcgttgtatt tatttgctga attaataaaa caatagcgaa agtctgccgg
      421 aggatgattt ctcattcacc ctataataca aagatgaagt atcgatgatg gatatggttg
      481 taaaagaaac aagcaatgaa atatcggagg tcattaatgg ataaaataat taaaataaga
      541 agagaagaac aaacagatta tgagagggta gaagaaatca caaggaaagc tttttggaat
      601 ttatatactc ccgggtgcaa tgaacactac ttagttcatg ttatgcgctc ccacaaggat
      661 tttctgccgg aattggattt tgttattgaa gttgataatc agattatcgg taatataatg
      721 tatacgaaag caagacttat tgatgaggca ggggaagaaa aaaatatcct tacttttggt
      781 ccggtcagca tcctgccgga ataccagaga agggggtatg ggaaaaaatt attggagtat
      841 tcttttgaac aagcggctgc tcttggttat gatgtaattg taatatttgg aaatccgggt
      901 aattatgtta gccgcgggtt taagagctgt aagaaattta atgtctgtct tgaggatggg
      961 acatatccga ctgcaatgtt ggtaaaagaa ctcaaaccta atgtattgga cggaagaaag
     1021 tgggtttatt atcagagtcc cgtatatgag atagatgaac aagaagctct gtgttttgac
     1081 gatgccttgg aaaaaatgga gaaaaaatac ctgccatgcc aggaagaatt ctatattctc
     1141 agtaattcta ttataaagta aacttctttt taaaataaac cgaggaggat gaaatgaaat
     1201 atacatttat ttgttattcg agatgaggta catgcaaaaa ggccaaggat tggttaataa
     1261 aaaatgatat tgattttacg gagcgatcca taaaggagga aaatccgaac gagagcgagc
     1321 ttatcgaatg gattaaaaaa ggcaaatgct ctataaagag attttttaat actagcggca
     1381 ctctgtataa ggatttaaac ctaaaagaaa aattagaaaa catgtctgat gaagaacaaa
     1441 tcaaactgtt gtcaacctat ggtatgctgg taaaaagacc catacttgtt ggcggggata
     1501 ttgtattagt cggatttaaa gaaaatgagt ggagggaaaa acttctttga caattgaaaa
     1561 atcaaagtag tgcgttttta ttaaacatag gtgaactcat gaatattaaa tacataaaag
     1621 aacttttatc aagaataaat gaagtaagaa taatcaacaa aaaggcagat attgaaggtg
     1681 ttgtctgtaa tattgcaggg atagtgcgat acggacaaaa attgcgtctt attatattgg
     1741 aatatgatga ggtgtatcgt caaaaagttg aagaaatgga actgtcagag ccttgtgagc
     1801 ttagacaaca agagacgaac cggtcaatac taatgggcag gggtagaata gaagctgcac
     1861 agccttttag acctataaaa agtgtatt
//
LOCUS       DAOSOW010000369         1886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_61449_length_1886_cov_3.395958, whole genome shotgun sequence.
ACCESSION   DAOSOW010000369 DAOSOW010000000
VERSION     DAOSOW010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61449_length_1886_cov_3.395958"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..1629
                     /locus_tag="PLD67_08515"
     CDS             274..1629
                     /locus_tag="PLD67_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HOT22606.1"
                     /translation="MEALMKINSAVNGIVWGPLIIVLIIGTGTYLSINTKFFSITKIG
                     YVLKNTLLKMFAKDDKGEGEVTAFQAVATALAATVGTGNIAGVATAIALGGPGAVFWM
                     WLAAIMGMTTKFAEVVLAVNYREKTPDGRFVGGPMYYITNGLGKNWKWMAVLFAFFGT
                     FASFGIGCMVQSNSVALSAQSTFGVPVMVTGIVLAILTGAVIIGGIKRIGAFTEKLVP
                     FMSAIYIVGGLWLIIANASQLPRAFSLIFTNAFTGTAAVGGFAGTVMMQAIRYGVARG
                     IFTNEAGLGSAPIAHAAATTDHPVRQGLWGVFEVFMDTIIICSITALSIMVTGVWESG
                     ESGAVLSAMAFDVGLPGVGKYIVSIGLILFAYSTILGWEYYGERCLEYLAGTKPIIAY
                     RLLWVVAVVVGAIGGLEFMWDLADTLNGLMAFPNLVGVLILSPVVFKLTREFFAKERT
                     H"
     gene            complement(1699..>1886)
                     /locus_tag="PLD67_08520"
     CDS             complement(1699..>1886)
                     /locus_tag="PLD67_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009354737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22607.1"
                     /translation="GIKENDIRWNFTKFLIDKEGNVVARFAPNITPEEMEEHVLRLLR
                     DEEIEIVCHSDFSNGCI"
BASE COUNT          554 a          319 c          387 g          626 t
ORIGIN      
        1 taatatactg ataccccctt ttattatttt caagcaaaat ttttactgtt ttgtttgaaa
       61 aaattttaac aatatccatc aaaaatagta aattaaatat gaaataaata taataaaaat
      121 tgtaataatt ttagttgaat tatttttata atgttatata atatatattg aaaacgttta
      181 attattttat tatttaatct ttattttaat cgttctaata cataaatact aaaatgtaaa
      241 cgtttatcta acattttgaa tggagggatt tgtttggaag cattaatgaa aatcaacagt
      301 gctgttaacg gcatagtatg gggtcctcta attatagttt taattattgg aaccggaact
      361 tatttgtcaa ttaacacgaa atttttctca attactaaaa taggctacgt acttaagaac
      421 accttgttaa aaatgttcgc caaggatgat aaaggcgaag gtgaagttac tgcattccag
      481 gcagttgcaa ctgctcttgc cgctacggtt ggtacaggta atattgcagg agttgcaaca
      541 gctatagcac ttggaggccc cggagcagta ttctggatgt ggcttgcagc aattatgggt
      601 atgacaacaa aatttgcgga agttgtattg gctgtaaact acagggaaaa aacacctgac
      661 ggccgttttg taggcggtcc catgtactac attacaaacg gtctgggtaa gaactggaaa
      721 tggatggctg ttttatttgc attcttcggt acttttgctt cctttggtat cggctgcatg
      781 gttcaatcaa attcagttgc actttcagca caatctactt ttggtgtacc cgtaatggtt
      841 acaggtattg ttttagccat attaacagga gctgttatta tcggaggtat taagagaatc
      901 ggtgctttca ccgaaaaatt ggttccattc atgtcagcaa tttatattgt cggcggtctt
      961 tggttgatta tagcaaatgc cagtcagtta cctcgtgcat tttcattgat tttcactaat
     1021 gcgttcacag gtacggctgc cgttggaggt tttgcaggta cagttatgat gcaggcaatc
     1081 cgttacggtg ttgctcgcgg tatctttact aacgaagccg gcttgggtag cgctcctata
     1141 gctcatgccg ctgcaactac agaccatcct gtacgtcaag gcttgtgggg agtattcgaa
     1201 gtatttatgg atacaataat tatctgctct attactgctc tttctattat ggttacaggt
     1261 gtttgggaat caggagaatc gggagcagtt ctttcagcaa tggcatttga tgtaggtctt
     1321 ccaggtgtag gtaagtacat tgtaagtatc ggtcttatat tgtttgcata ctcaacaatt
     1381 ttaggatggg aatattatgg tgaaagatgc cttgaatatc ttgcgggaac aaagccaata
     1441 atagcataca gattgctatg ggtagtagcc gtagtagttg gagctatagg cggacttgaa
     1501 tttatgtggg acttggcaga cacattaaac ggattgatgg catttccaaa cttggtaggg
     1561 gttcttatat taagcccggt agtatttaaa ttgacaagag aattctttgc aaaagaaagg
     1621 acacattaaa gaagaaagaa atcaaggcgc tgacaactca gcgccttcta atgcaatttg
     1681 tattgctgtt ccagtccctt aaatgcagcc gtttgaaaaa tccgaatgac acacaatctc
     1741 aatttcttca tcacgaagca acctcaaaac atgctcttcc atttcttcag gcgtaatgtt
     1801 tggtgcaaat cttgctacaa cattcccttc cttatcaatc aggaatttgg taaaattcca
     1861 tctgatatca ttttctttaa ttccgg
//
LOCUS       DAOSOW010000370         1886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_61450_length_1886_cov_3.372474, whole genome shotgun sequence.
ACCESSION   DAOSOW010000370 DAOSOW010000000
VERSION     DAOSOW010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61450_length_1886_cov_3.372474"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..600
                     /locus_tag="PLD67_08525"
                     /pseudo
     CDS             1..600
                     /locus_tag="PLD67_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966587.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
     gene            complement(900..1748)
                     /locus_tag="PLD67_08530"
     CDS             complement(900..1748)
                     /locus_tag="PLD67_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HOT22608.1"
                     /translation="MRRKKKSNIKKTLIAVTALLFILFAVLLIKEKLNNDKLPPQEVQ
                     EEENPPNNQNKPEDEEETSTEPEKEPQEEEPKEPEPEQVPEDTEYGIIIQNPEKIDVL
                     VNRKRNLPDTYVPEDLVTLSEVPTVLSNPEVNQLRSAAYEALKELFNAAWEEAGFELC
                     ARSGYRSYNTQSSLYTSYVESYGQQAADKYSAKPGQSEHQTGLAIDITCEALNYKLDT
                     TFAETEEGKWVSENAHRFGFIIRYPKGKEEITGYAYEPWHIRYLGVELAEEIYESGLT
                     LEEYFQ"
BASE COUNT          570 a          367 c          335 g          614 t
ORIGIN      
        1 cggatgaagg aagaccaaaa gtatatctat tatgccaccg tagaaagtat cgacaagctg
       61 tcaaagctcc cttaggtaga gctcatcgct gacaagggat ttgaaattct ctattttaca
      121 gatgaaatag atgaatttgc aataaaagtt ttattaaact acaaagacaa agagtttaag
      181 aatatatcaa gctccgacct ggatattaaa tcagaagatg acaataccga caatgttgaa
      241 gaaaataaag agttgtttgc ttttatgaag gaatcacttg gtgaaaaagt taaagaggtg
      301 agagcttcaa aaagactcaa aactcatccc gtgtgccttg ctaccgaagg tgaattatcg
      361 atagaaatgg aaaaggtgtt aaatgcactg cctgataaac cttcaagcat ccatgcggag
      421 aaaattcttg aaattaacac cagccatcaa atttttgaaa aattaaaaga tgcatttcat
      481 aatgataaag aaaaatttaa aaagctttca aacgtattat ttaatcaagc attgcttatt
      541 gaagggctta caatcgaaga ccccgtccaa tatgcaaatg atgtatacga gctgatataa
      601 aaaggccatg gaatcatgtg gctcaggaca ctaaaaaaaa taaaaatatc cgctcacggt
      661 ttcggccacg aataattcta ggagctcatt ccggctaaaa atcctacagc ttgcgaactc
      721 gctctgctca aacaacgcaa gctgtttaac ggatttttaa cctgcattcg ctaagaatta
      781 ttatcgtgtc ctgcaaatgt ttgcttgata tttttatttt tcttagaaat aagcgaagga
      841 tttcataata atgggttata actttccatc aaataaaacc acggggattc cgtggctttt
      901 tattgaaaat attcttccaa tgtgagtccc gactcatata tttcttctgc cagctcaaca
      961 cctaagtatc ttatatgcca tggctcatat gcataacccg taatttcttc cttcccctta
     1021 ggatatctga taataaatcc gaaacggtga gcgttttctg aaacccactt gccttcttct
     1081 gtttcggcaa atgtagtatc caatttgtaa tttagggctt cacatgtaat atctattgca
     1141 agacctgtct ggtgttcgct ttgacccggt ttggcactgt atttatctgc cgcttgctga
     1201 ccgtagcttt caacatatga agtatacaat gatgattgcg tattatatga cctgtagcct
     1261 gagcgtgcac acagttcaaa accggcttct tcccatgcag cattgaacaa ttccttcaat
     1321 gcctcatagg cggcacttct taattgattt acttccgggt ttgaaagcac tgtaggtact
     1381 tcgctaagcg ttactaaatc ttcaggcaca taggtatcag gcagatttct ttttctattt
     1441 accagaacat ctattttttc aggattttga attattatgc catattcagt atcttcaggc
     1501 acctgttccg gttcaggctc ttttggctct tcttcctggg gttctttttc cggttcagtt
     1561 gaagtttctt cctcatcctc gggtttattt tgattgtttg gaggattctc ctcctcctga
     1621 acctcttgag gaggtaattt atcattattt aatttttcct ttatcagtaa tactgcaaat
     1681 aaaataaaga gcaaagcagt aacagcaatt aatgtctttt ttatattact tttctttttt
     1741 cttctcatta ttttctccta tgttaatgtc attccggtca tagtcacttg cttaacgaat
     1801 gataaacagt tatattttgg gatgtcatcc tgagcgtaag cgaaggatct catgagattc
     1861 ttcgagctaa agctctcaga atgaca
//
LOCUS       DAOSOW010000371         1883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_61584_length_1883_cov_3.434354, whole genome shotgun sequence.
ACCESSION   DAOSOW010000371 DAOSOW010000000
VERSION     DAOSOW010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61584_length_1883_cov_3.434354"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1798)
                     /gene="fliD"
                     /locus_tag="PLD67_08535"
     CDS             complement(<1..1798)
                     /gene="fliD"
                     /locus_tag="PLD67_08535"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HOT22609.1"
                     /translation="MSILSTNLYGSSVNKGMGGLMSGLDTDDLVNQMTAATRNKINKQ
                     YQAKQKLLYKQEAYREISSKLLAFNNKYFSYSSGSKTNILSPKFFESYTYKSSSDYVS
                     VTGNADVISNFKINSITSVAKAATYTTNSVSSQSFSSDAIIGNISEDVLLSSSITFDF
                     NGVKKTIALSEMVDGKPDGSPYTYDAEGLADFLNTKLAEVYGAGRVNAELDASQTSIM
                     FSASGSTDVFSVSNISNDLSILTGIKSGDSNRLKVSKTIGDIYGEVTDCSIIINGKSI
                     DGISKDMSISEVLKKINKESDVDITYSNTTDKFVIVSKDTGKASKIEITGALADKLFG
                     GSIGTDIEITGEDTVMNVTIHGQDIDITRSTANFSIDGINIELSKNAILAPEEDPITF
                     DVINNTDEVIERVKQFIDDYNEIIDLLGTKTKEKPRRDYPPLTPEQQDEMKEKEIENW
                     LKEAKKGVLFGDKNITSLLNKMRLSMSGMTSVSDLALSNIGISAASMDTSGKLVFNEE
                     KFREKLIEAPDQIASLFTGIADETDEYAKSGIANQILDILRENIGAMGTSGKLIEEAG
                     LDTGRSSDQNNISLKIKDYDEKMNELKKSLERE"
BASE COUNT          517 a          364 c          279 g          723 t
ORIGIN      
        1 tttctctttc gagactcttc tttaactcgt tcattttttc atcataatct ttaattttta
       61 acgaaatgtt attttggtcg gatgaacgac cggtgtctaa gcctgcttct tcgattaact
      121 ttccggatgt acccatagct ccaatatttt ctctaagtat atcaagaatt tgatttgcaa
      181 tgccggactt tgcatattca tccgtttcat cagctatacc tgtaaataag cttgctattt
      241 gatcgggagc ttctattaac ttttctctga acttttcttc attaaaaacc aatttaccgc
      301 tggtatccat tgatgctgct gaaattccga tattggacaa agccaagtcg cttacggagg
      361 tcataccgct catacttaat ctcattttgt tcaataggct tgtaatattc ttatcgccga
      421 agagtactcc ctttttagct tcctttaacc aattttctat ttctttttct ttcatttcat
      481 cctgctgctc gggagttaat ggaggataat ctcttcttgg tttttctttt gttttagtgc
      541 cgagtaaatc tattatttca ttataatcat caataaactg cttcactctt tcaatcactt
      601 catcagtgtt atttattaca tcaaaagtta tagggtcttc ttcaggtgca agtattgcat
      661 ttttgctcaa ttcaatattt ataccatcaa ttgagaaatt agcagtgctt cgtgtaatat
      721 ctatatcttg cccgtgaatg gttacattca tcactgtgtc ttcgcctgtt atttcaatgt
      781 cagtgccaat acttcctccg aaaagcttat ctgccaatgc tcccgtaatt tcaatttttg
      841 atgctttacc ggtatcttta gacacgataa caaatttatc cgtggtattg gaatatgtaa
      901 tatcaacatc agattctttg tttatctttt taagcacttc ggaaattgac atatccttac
      961 taattccatc tattgatttt ccgttaataa taatagaaca atctgttact tctccatata
     1021 tatcacctat agttttcgaa acttttaatc tgttgctgtc ccctgattta attcctgtta
     1081 gtatgcttaa atcattgctg atattgctta cactaaaaac atcggtactt ccggaagcag
     1141 aaaacattat tgaagtttgg gaagcatcaa gttctgcatt tacccttccg gctccataaa
     1201 cttcagcaag ttttgtattc agaaaatctg ctaatccttc agcatcataa gtataaggtg
     1261 aaccgtcagg cttaccgtca accatttcag aaagagctat agtcttcttc actccgttaa
     1321 aatcaaatgt aatgctgctg cttaataaaa catcttccga tatgttgccg attattgcat
     1381 cacttgaaaa gctttgagat gaaacagaat ttgttgtata tgttgcagct ttagcaaccg
     1441 atgttatact attgatttta aaattactta taacgtctgc attgccggtc acgcttacgt
     1501 aatcagatga ggatttatat gtatatgatt caaaaaactt aggactaaga atattagttt
     1561 ttgagccgga tgaataagag aaatatttat tgttaaaggc tagaagcttt gaacttattt
     1621 ctctgtatgc ttcctgtttg tacaacaatt tttgttttgc ttgatattgc ttatttattt
     1681 tattcctggt ggcggctgtc atttgattga ccaagtcatc ggtatccaat cctgacataa
     1741 ggccgcccat tcctttgttt acagatgagc cgtataaatt agttgaaagt attgacatat
     1801 ctttctcctt ttcttctata ttataatttc catgtcttaa tatctatcta ttcatatatc
     1861 gtcaataatc caaataaatt aaa
//
LOCUS       DAOSOW010000372         1874 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_62020_length_1874_cov_5.030236, whole genome shotgun sequence.
ACCESSION   DAOSOW010000372 DAOSOW010000000
VERSION     DAOSOW010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62020_length_1874_cov_5.030236"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..1099
                     /locus_tag="PLD67_08540"
     CDS             71..1099
                     /locus_tag="PLD67_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22610.1"
                     /translation="MRLIETIKQNNYETVSIIGLAKNAGKTVTLNYLIEEAINLNIKT
                     GITSTGRDGENTDLITKTQKPAISVTEGMYVATAKKTLMLSNAKAEILETTGISTAMG
                     EVIIIRVRQKGNIQIAGPVSAKDMKYVARRLKYYGAEVVFIDGAIDRKAVSSPAITDA
                     CVIATGAVLSRDMKKVLEKTAHAVECYSLTGTDEHVKNIVRKINKTCIISEDGKTLVP
                     DIKTSITSGKKISEMISEKTTHVFVKGAVTSALLKDLWENKYLRGIKLIIEDGTKIFT
                     DINMWNEMRRKGLKVETINTINVLAVTLNPVSPEGYFFDSEVLKENMKKVLTGIKIVD
                     VVSGGEED"
     gene            1096..>1874
                     /locus_tag="PLD67_08545"
     CDS             1096..>1874
                     /locus_tag="PLD67_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22611.1"
                     /translation="MNSFMTENTIKNVNFDYIFNEVKPITEYGIRTKQEAKPFIKGQE
                     ADLKAEFNKIGAFIESRQRRDIIDVLKHIKNILETIQRAKSNQVLDEVELFEIKNFLI
                     LVEKMDKILRGISNNDLKLLQLTPLPQLYEKLDPAHEKLNTFYIYDEYSQKLKKIRSE
                     KRQIEKHISLLKKRIREEIQEKHNIKLNLKDEVTISKSQKDKINELNKEVNLRVFGEN
                     YLNIIYKIKNSEEIDELEKRYEELTFEEDDEEYKIRQKLSVS"
BASE COUNT          772 a          250 c          382 g          470 t
ORIGIN      
        1 ggagcagacg aaattgtgca gcgagactgc gctataatga taacaatgaa ctgactataa
       61 gaggttttct atgagattaa ttgaaaccat aaaacaaaat aattatgaaa ctgtatccat
      121 aataggtctt gcaaaaaatg caggaaagac cgtgacactt aattatctca tagaagaagc
      181 aataaattta aacattaaaa caggtattac ttcaacaggg cgcgacggtg aaaatacaga
      241 tttaataaca aaaacacaaa agcctgcaat ttcggtaact gaaggaatgt atgttgccac
      301 tgcgaaaaaa acattgatgc tttcaaacgc taaggctgaa atattggaaa ctacggggat
      361 atcaactgcc atgggcgaag taataattat aagggtgaga caaaaaggca atatacagat
      421 tgccggacct gtcagcgcaa aagatatgaa atatgttgcg aggcgcctta aatattacgg
      481 agcagaagtg gtattcatag atggagcaat agacaggaag gctgtttcat ctccggctat
      541 tacagatgca tgtgttattg caacaggtgc tgtacttagc agagatatga aaaaggtttt
      601 agaaaagact gcccatgctg tggaatgcta ctcgcttaca ggtacggatg agcatgttaa
      661 gaatatagtg cggaaaatca ataaaacatg catcatatca gaagacggta aaactcttgt
      721 tcccgatatt aaaacaagta ttacgagcgg caaaaaaatc agtgaaatga ttagtgaaaa
      781 aacaactcac gtgtttgtta aaggtgcggt tacatctgcc ttgttgaaag atttatggga
      841 aaacaaatat ttaagaggca ttaaattaat tatagaggac ggaacaaaaa tatttacgga
      901 cataaatatg tggaatgaaa tgagaaggaa aggacttaaa gttgaaacca taaatacaat
      961 aaatgttctg gcggtaactt taaatccggt atctccggaa ggatatttct ttgacagtga
     1021 agttttaaaa gaaaatatga aaaaagtcct gacgggaata aaaatagttg atgtggtgtc
     1081 gggcggtgaa gaagattgaa tagctttatg acggaaaata caataaagaa tgtaaatttc
     1141 gattatattt tcaatgaagt aaaaccgatt acagaatacg ggataaggac aaaacaggaa
     1201 gctaagcctt ttataaaagg tcaggaggca gaccttaagg cggagttcaa taaaatcggg
     1261 gcatttattg aaagcagaca aaggcgggac ataatcgatg ttttgaagca cattaaaaat
     1321 atactcgaaa ctatccaaag ggcaaaaagc aatcaggttc ttgatgaagt agaattgttt
     1381 gaaataaaaa actttttgat tttagtcgaa aagatggata aaattttaag aggcatttcc
     1441 aataatgatt taaagctatt acaattgacg cccctgccac aactatatga aaaacttgac
     1501 ccggctcatg aaaagttaaa cacattttat atttacgacg aatattcgca aaaattaaaa
     1561 aaaattcgaa gtgaaaaaag acagatagag aaacatatca gtttattaaa aaaaagaatc
     1621 agagaagaaa tacaagaaaa gcataatatt aaattaaatt taaaagatga agtaacaata
     1681 agcaagtcac aaaaagataa gattaatgag ctgaataagg aagtcaattt aagagtattc
     1741 ggagaaaatt atttaaatat tatttataaa attaaaaatt ctgaagaaat tgatgagctg
     1801 gagaaaaggt acgaagaact cacttttgaa gaagatgacg aagaatacaa aattcgacaa
     1861 aaactctcgg tgtc
//
LOCUS       DAOSOW010000373         1873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_62075_length_1873_cov_3.053905, whole genome shotgun sequence.
ACCESSION   DAOSOW010000373 DAOSOW010000000
VERSION     DAOSOW010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62075_length_1873_cov_3.053905"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /gene="fusA"
                     /locus_tag="PLD67_08550"
     CDS             <1..1520
                     /gene="fusA"
                     /locus_tag="PLD67_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOT22612.1"
                     /translation="ATKYNNDEGTALEVIDIPEDYLDKAQEYREKLIEALSDFNEEIM
                     EKYLNGEEISIAELKKAIRACTIKNEIVPVLCGTAYKHKGVRLMLDAVVDYLPNPLDV
                     PQLSGFDPHTNEVIVRDASDDAPLSALAFKIATDPFVGKLTFFRVYSGVLQSGSYVLN
                     STKNKKERIGRILQMHANKREEIDNSYTGDIDAAVGLKITTTGDTLCDPDHPIVLESM
                     VFPEPVIEVAIEPKTKAGQEKMGIGLQKLAEEDPTFKTYTNPDTGQTIIAGMGELHLE
                     IIVDRLLREFKVEANVGKPQVSYKETVTGKADVDVKYARQSGGKGQYGHVKITMEPQE
                     PGQGITFVNAIVGGVIPKEYIPAVEAGIKGAAENGILAGYPVIDFKVTLYDGSYHEVD
                     SSEMAFKIAGSMAFKQAMEKASPVLLEPYMKVEITTPEEYLGDVMGDVNSRRGRIEGF
                     SDRLGIKVVDAYVPLSEMFGYATTLRSLTQGRASYSMEFHHYEKVPNNIAEKIVK"
     gene            1567..>1873
                     /locus_tag="PLD67_08555"
     CDS             1567..>1873
                     /locus_tag="PLD67_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005958112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT22613.1"
                     /translation="MAKAKFERNKPHVNIGTIGHVDHGKTTLTAAITLILNKRFGTGE
                     FVSYDNIDKAPEERERGITISTSHVEYETQNRHYAHVDCPGHADYVKNMITGAAQMDG
                     "
BASE COUNT          678 a          319 c          417 g          459 t
ORIGIN      
        1 aagcaacgaa atataataat gatgaaggga cagctctgga agttatcgat atacctgagg
       61 attatttgga taaggcacag gaatatagag agaaacttat agaagcttta tcggatttta
      121 acgaagaaat aatggaaaaa tatttaaacg gcgaagaaat ttcaattgca gagttaaaaa
      181 aagcaataag agcatgcact attaaaaatg aaattgtacc ggtattatgc ggtacagctt
      241 acaaacataa aggcgtaaga cttatgctgg atgcagttgt tgattatttg cctaatccgt
      301 tagatgttcc tcagttgagc ggatttgatc ctcacactaa tgaagtaata gttagagatg
      361 cgtcagatga tgctccatta tcagctttgg catttaaaat agcaacagac ccctttgtag
      421 ggaaacttac attcttcagg gtttattcag gggtattgca atcaggctct tatgtcttga
      481 attcaaccaa gaacaaaaaa gaaagaatag gcagaatact tcaaatgcat gccaataaga
      541 gggaagagat tgacaactct tatacgggag atattgatgc tgccgtcggt ctaaaaatca
      601 caacaacagg agataccttg tgtgaccctg accaccccat tgttctggaa tccatggtat
      661 tcccggaacc tgttatagag gtggcaatag agccaaaaac taaggcagga caggagaaaa
      721 tgggtatcgg acttcaaaag ttagctgaag aagatcctac ttttaaaaca tatacaaatc
      781 cggatacagg acagacaatt attgcaggaa tgggcgagct gcatttggag ataatagtcg
      841 acagactgtt aagagaattc aaggttgaag cgaatgtagg caagccgcaa gtttcttaca
      901 aagaaacagt aacaggcaaa gcagacgttg acgttaagta tgcaagacaa tccggaggca
      961 agggacaata tggtcatgtt aaaattacca tggagcctca agaacccggt caaggcatta
     1021 cttttgtcaa tgctatcgta ggaggagtta ttcctaaaga atatataccg gcagttgaag
     1081 ccggaattaa gggcgctgca gaaaatggta ttttagccgg ttatccggta atagacttta
     1141 aagttacact gtatgatggt tcataccatg aagttgactc gagcgaaatg gcatttaaaa
     1201 ttgccggctc aatggcgttt aaacaagcga tggaaaaagc atcgccggtg ctcttagagc
     1261 cttatatgaa ggttgaaata acaactcccg aagaatactt gggagacgtt atgggagacg
     1321 taaactcaag aagaggaaga atagaaggtt tctcggacag actcggtatt aaagttgttg
     1381 atgcttatgt tccgttatcg gaaatgtttg gatatgcaac gacacttcgc tcgctgactc
     1441 aaggaagggc ttcttattca atggaattcc atcattacga aaaagtgccc aacaatatag
     1501 ctgaaaaaat tgtaaaataa aacaaaatat aacattataa ataaacagcc taagggagga
     1561 aaagaaatgg caaaagcaaa attcgaaaga aataaacccc atgttaatat aggaacaatt
     1621 ggtcacgttg accacggcaa aactacatta acagcagcaa ttacacttat tttgaacaaa
     1681 agattcggaa cgggagaatt cgtaagctat gacaacatag acaaagctcc tgaagaaaga
     1741 gaaagaggaa ttacaatttc aacttctcac gttgaatatg aaactcaaaa cagacactat
     1801 gcacacgttg actgtcctgg tcacgctgac tatgttaaga acatgattac cggagcagct
     1861 cagatggacg gag
//
LOCUS       DAOSOW010000374         1872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_62116_length_1872_cov_3.424876, whole genome shotgun sequence.
ACCESSION   DAOSOW010000374 DAOSOW010000000
VERSION     DAOSOW010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62116_length_1872_cov_3.424876"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PLD67_08560"
     CDS             complement(<1..681)
                     /locus_tag="PLD67_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22614.1"
                     /translation="MLVTKWKIFKKKYITKFSAFILLIVFLMIGVSSALNIYLKVKNY
                     ESIAVKNYLESDTISNDLKYAVNRLEFILRVYKNEEYILSGGTVEKLEIEDNWKLVNL
                     YNNYLAENNYEDNSETKELFFIEKQKEIQEIKESIINSDLANYEKIISELNLPQGYIY
                     YATDGENEITNTGNPNKEFYLLRNAYIVLSEKGVELSPENGGGVTDPLTDIFESETFN
                     KNMRIYAAI"
     gene            complement(653..1348)
                     /locus_tag="PLD67_08565"
     CDS             complement(653..1348)
                     /locus_tag="PLD67_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009004609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT22615.1"
                     /translation="MNNIMVCDDDKEIVDAIEAYLKSEGYNILKCYSGKQAIEIVEKN
                     EVHLIIMDIMMPEMDGITATTKIREIKNIPVIMLSAKSEEADIVLGLNMGADDYITKP
                     FNPLELIARVKSHLRRYTALGGALDAEKVGLYKTGSLMIDDERKEVTVDGEIVKLTPV
                     EYKILLLLTKNKGVVYSIDEIYENVWNEPSFNPENTVAVHIRRIREKIEIDPKNPRYL
                     KVVWGVGYKVEDI"
     gene            complement(1562..>1872)
                     /locus_tag="PLD67_08570"
     CDS             complement(1562..>1872)
                     /locus_tag="PLD67_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="endoglucanase"
                     /protein_id="HOT22616.1"
                     /translation="YRGEIWRSFSEAYDHFYFDYIDRFTHPMMITEFSCASAGGDKAQ
                     WFEDMFNMIGKYDRIKLAVLWNGQDYDPNKEEKTVSRNYRIDLEESVINAVRNGLKSY
                     "
BASE COUNT          519 a          384 c          253 g          716 t
ORIGIN      
        1 tatggcggca taaatccgca tatttttatt aaaggtttcc gattcaaata tatcggtcaa
       61 ggggtcggtt acaccgcctc cgttttcggg actgagttca acacccttct cgcttaagac
      121 aatgtatgca ttgcgcagta aataaaattc tttgttagga ttgcctgtat ttgttatttc
      181 attttcaccg tctgttgcat aataaatata accctggggc aaattcagct cactgattat
      241 tttttcatag tttgccaagt ccgagtttat tattgattct tttatttctt gaatttcctt
      301 ctgcttttca atgaaaaata attcttttgt ttcagaatta tcctcgtaat tattttccgc
      361 cagatagtta ttatatagat ttacgagttt ccaattatcc tcaatctcta atttttcaac
      421 ggttcctccg cttaaaatat attcttcgtt tttatatact ctcaatataa attcaagacg
      481 atttacggca tattttaggt cattgcttat ggtgtcgctc tctaaatagt tcttaacagc
      541 aatgctctca taatttttaa cctttaaata aatattcaaa gctgaagaaa ctcctatcat
      601 aagaaagact atcaaaagta taaatgccga aaattttgtt atatattttt ttttaaatat
      661 cttccacttt gtaaccaaca ccccacacca ccttaaggta tcttggattt tttggatcaa
      721 tttcaatctt ttctcttatt cgtcttatgt ggacggccac tgtgttttca ggattgaaag
      781 aaggctcgtt ccacacattt tcatatattt cgtctatgga ataaacaact cctttgtttt
      841 tggttaacaa aagtaatatt ttgtattcaa ctggtgtaag ctttacaatt tctccgtcta
      901 ccgtaacttc ttttctctca tcgtcaatca tcaaacttcc tgttttatac agtccgacct
      961 tttccgcatc caaagctccg cctaatgccg tataccttct aaggtgggat ttcacccttg
     1021 caataagctc caaaggattg aatggcttag ttatataatc atccgctccc atattaagcc
     1081 ctaatactat gtctgcttct tcggattttg ctgacagcat aataacaggg atatttttaa
     1141 tttccctgat ttttgttgtt gctgttatgc cgtccatttc cggcatcatt atatccatta
     1201 ttattaaatg cacttcattt ttttcaacaa tttcaattgc ctgttttcca gaatagcatt
     1261 ttaaaatatt gtatccttca ctttttaaat aagcttcaat tgcatctact atttctttat
     1321 cgtcgtcgca aaccattatg ttgttcatct tatccctcct gtattttata atgtcatcct
     1381 ttgatatcac ggctatgatt ataagattgc tttaagctat tatcatatca cattattctt
     1441 aaaatataat taaaaaagcg gggtgcgtcc cccgcaactt attacaacta taaatttggc
     1501 tttgagattc ttcgagctaa agctctcaga atgactatac tccgattatt cattgcaata
     1561 atcagtatga cttaagtccg tttcgcactg catttataac ggattcttcc aagtcaattc
     1621 tgtaatttct cgatactgtt ttttcttcct tgttcgggtc atagtcttga ccattccata
     1681 gaactgccaa ttttatacgg tcatatttac caatcatatt aaacatatct tcaaaccact
     1741 gagctttatc tcctcctgca gaagcacaac tgaattcggt tatcatcatg ggatgagtaa
     1801 atctatctat atagtcaaaa taaaaatggt catatgcttc ggagaaactc ctccaaattt
     1861 ctcctctgta at
//
LOCUS       DAOSOW010000375         1866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_62378_length_1866_cov_3.427388, whole genome shotgun sequence.
ACCESSION   DAOSOW010000375 DAOSOW010000000
VERSION     DAOSOW010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62378_length_1866_cov_3.427388"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="PLD67_08575"
     CDS             complement(<1..665)
                     /locus_tag="PLD67_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22617.1"
                     /translation="MTRKITVWFLIVSMIIMNATFVFAAEPELIITGTGVEEEVVISS
                     GDWSKYKMVERIYSTNNSLGFHKIIKAKGYDLFELMGNGLKTDKDYSVKFTCSDGFEF
                     TKTISELKSAYYFSDFTLESKQAVMPMVAKYTTVMADFPKNIFAPPLTWTDIPITEKD
                     LDKDFPKLVFGQTDIDDMNMSKWGKEVIKITVGDEIVKVTPSSPYKHISYEGEPYNVD
                     AVTG"
     gene            complement(717..>1866)
                     /locus_tag="PLD67_08580"
     CDS             complement(717..>1866)
                     /locus_tag="PLD67_08580"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT22618.1"
                     /translation="KFKVIAMVLAIVMILSQSLVFAEDYAIEIGGTLVATELKLTLED
                     LKSMPEEAQIAQEYVYNSKGGEKTVQVKGVSLAYLLKEMAGVTAEEGNVEFFAADEWP
                     IDPQPLQDVLNDELKFVLAYEVDGEAIDNDEGIIEEITVYRNLKYEGEFDTVYKLINK
                     ITVGEAEAVEEEEAPEEEPIEPIEEETEETEETEEVTFTDITEEYKFAEAAIYDLAKR
                     GIIDGMGGGLYAPGNEFTREQFCKIIVVALEYELKEYEGAFSDIDDDRWSAPYVQAAV
                     DSGLFVGYPDGTFLPEKVITRQEMALVAARAAVAKGLVEQAKVDKFVMEKSDYIDKED
                     VADWAGNAVAWLEAQDVFAGIAVEKFEPARNVNRAEAALVVFNTLFSE"
BASE COUNT          501 a          435 c          266 g          664 t
ORIGIN      
        1 cctgttacgg catccacatt gtaaggctca ccttcatagc tgatatgttt atatggtgat
       61 gaaggagtta cctttactat ttcatctcct accgtaattt ttataacttc cttgccccat
      121 ttagacatat tcatatcgtc aatgtcagtt tgtccgaata caagttttgg aaaatcttta
      181 tctaaatctt tttccgtaat gggaatatcc gtccatgtaa gaggaggagc aaaaatattt
      241 ttcggaaaat cagccattac cgtagtatat tttgcaacca taggcataac tgcctgtttg
      301 ctttccaaag taaaatcact gaaataataa gcgcttttaa gctcgctgat agtctttgta
      361 aattcaaaac cgtcagagca tgtaaatttt acgctgtagt ctttatcggt tttcaaaccg
      421 tttcccataa gttcaaataa atcatatcct tttgccttta tgattttatg aaatccaagg
      481 ctgttgttgg tggagtaaat tctctctacc atcttatatt ttgaccagtc accggatgag
      541 attactactt cttcctccac tcccgtgcct gtaattataa gttcaggctc tgctgcaaat
      601 acaaacgttg cattcattat aatcatggat acaatcaaga accagactgt aatttttctt
      661 gtcatttacc ttacctcctt taaaataaat gagcgaatat cgctcattta ttttgattat
      721 tccgaaaaca atgtattgaa tactaccaag gctgcttcgg cacgattaac attccttgca
      781 ggctcaaatt tttctactgc aatccctgca aacacatcct gtgcttcaag ccatgcaact
      841 gcatttcctg cccagtctgc cacatcttct ttgtctatgt aatcagattt ttccattaca
      901 aatttatcaa cttttgcttg ttcaaccaat ccttttgcaa ctgctgccct ggctgctaca
      961 agtgccattt cctgtcttgt tataaccttt tcaggtaaaa atgtaccgtc aggatatcct
     1021 acaaacaaac ccgaatcaac tgcagcttgt acatatggtg ccgaccatct gtcatcatct
     1081 atatcgctga atgctccttc gtattctttc agctcatatt ccaatgccac aactataatt
     1141 ttgcagaatt gttctcttgt aaattcattt ccgggagcat agagtccgcc tcccattcca
     1201 tcaatgattc ctcttttggc gaggtcataa atagctgcct ctgcaaattt atattcttcg
     1261 gttatatcag tgaatgttac ttcttctgtt tcttctgttt cttcagtttc ctcttcaatc
     1321 ggctcaatag gctcttcttc gggtgcttct tcttcttcca ctgcttcagc ttcccctaca
     1381 gtaattttat taattagttt ataaacagta tcaaattcac cttcatattt taaatttctg
     1441 tatacagtta tttcttcaat tattccttca tcattgtcaa ttgcctcacc gtctacctca
     1501 taagccaaaa caaatttaag ttcatcgttc agaacatcct gaaggggctg tgggtcaatc
     1561 ggccattcat cggcagcgaa aaattctaca tttccttctt cagcagttac tcctgccatt
     1621 tctttcaata aataagctaa acttacaccc ttaacctgga ctgttttttc tcctccctta
     1681 gaattgtaaa catactcctg tgctatttgc gcttcctcgg gcatagattt taaatcttca
     1741 agggtaagct taagctctgt tgcaactaat gtccctccga tttcaattgc ataatcttct
     1801 gcaaaaacca atgattgcga aagaatcatc actattgcca ataccattgc aattacttta
     1861 aatttt
//
LOCUS       DAOSOW010000376         1839 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_63678_length_1839_cov_3.371076, whole genome shotgun sequence.
ACCESSION   DAOSOW010000376 DAOSOW010000000
VERSION     DAOSOW010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63678_length_1839_cov_3.371076"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLD67_08585"
     CDS             complement(<1..331)
                     /locus_tag="PLD67_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22619.1"
                     /translation="MNKLGKDCMPCTVKRSMAIILTLMMIISTLLNSGNLIYAFGDGE
                     MVLKSANHTETVRGAVIEGTTVTFTVPFGYTEDKLDLSDISYELEAGFEKVDIEFPSG
                     YEAVIGGS"
     gene            complement(497..1666)
                     /locus_tag="PLD67_08590"
     CDS             complement(497..1666)
                     /locus_tag="PLD67_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOT22620.1"
                     /translation="MLLKEMREKLDAQELKDNKDYLLTIAAGASNYYIENTEADKFQN
                     YLDYVNLMTYDIHGPWDKYTDFNSPLYNNNDESHQYKISIESSVKAWLRAGLPAEKLV
                     VGVPFYGYLYTVLKNTIDGLYQTHKNGKALSYDTIKKEYLSNPQYTKNFHYESLVPWL
                     NNGKTYISYDDSQSIALKAVYIREQNLGGAMIWELSQDSEGELLNSLYEGLYDGSVLA
                     ARDYVGHWAEAVIQKWLDMEYITGYPDGTFRPEEFITRAEFVKVVNNAFGFKETAEIT
                     FTDVKEEKWYYEEIQKAYKEGDIVGVSETEFAPENYITREQAAIIMTRLLELEGNEKG
                     ADLFSDSSKISSWAKEYVGAAAEHNLIKGYEDNTFRPQNNIKRAEALVLLDRILK"
BASE COUNT          537 a          363 c          277 g          662 t
ORIGIN      
        1 tagaaccgcc aataacagct tcataacctg aaggaaactc aatatccacc ttttcaaatc
       61 cagcctctaa ctcataactt atatctgata aatctaattt gtcctcggta tagccaaatg
      121 gaacagtaaa tgtcacagtt gtaccctcta taacagctcc tctcactgtt tcagtatgat
      181 tggctgattt tagaaccatt tctccatcac caaaagcgta aattaaatta ccggagttta
      241 acaatgttga tattatcatc atcaatgtaa gaattatcgc catacttctt ttaactgtac
      301 atggcatgca gtctttccct aatttattca tagttacccc tcactttatc tttaatatta
      361 tatttcatgc atataaccta aacaattaga aacataaaat atacctgact atattgtagt
      421 acaatttttg attattagca atgggaaaaa ggaaaaaggt gcctggcacc gtggtgatgt
      481 ggtgcctggc acctttttat ttcagtattc tgtcgagtaa tacaagagct tcggctcttt
      541 ttatattgtt ttgcggtcta aatgtattgt cttcatagcc tttaatcaaa ttatgctccg
      601 ccgctgcacc cacatattcc tttgcccagc tgctgatttt gctgctgtcg gagaataaat
      661 cggcaccttt ttcatttcct tctaattcaa gcagtcttgt cataattatt gccgcctgct
      721 ctctcgtaat ataattttcg ggggcaaact ctgtttccga aactcctact atatctcctt
      781 ctttatatgc cttttgtatt tcttcatagt accatttttc ttctttaaca tccgtaaatg
      841 taatttcggc agtttcttta aaaccaaagg cattattaac tactttgaca aactcggctc
      901 ttgtgataaa ttcttcaggt ctgaaagtcc cgtcaggata tccggttata tattccatat
      961 caagccattt ctgtattacg gcttctgccc aatgtcccac atagtctctt gccgccaaca
     1021 ccgagccgtc atagagacct tcatacagtg aatttaaaag ctcgccttct gaatcctggc
     1081 ttaattccca aatcattgca ccgcctaaat tttgctctct tatatataca gccttcaatg
     1141 ctatagactg agaatcatca tagcttatat aagtttttcc gttgttaagc catggaacca
     1201 atgactcata atgaaaattt tttgtatatt gaggattgct gagatattcc ttttttatgg
     1261 tatcgtagct caatgctttg ccgtttttat gagtttgata gagcccatca attgtatttt
     1321 ttaataccgt gtataaataa ccgtagaacg gaactcccac taccagcttt tcagccggca
     1381 aacctgctct tagccatgcc ttaacacttg attcaatgct tattttatat tgatgtgatt
     1441 catcattatt gttatacaag ggagaattaa agtcagtata tttatcccaa ggaccatgta
     1501 tgtcataggt cattaggttt acataatcca aataattttg aaatttatct gcttctgtgt
     1561 tttctatgta atagttgctt gctccggctg caatagtgag caaataatct ttgttatcct
     1621 tcaattcttg agcatccagc ttttcgcgca tttccttaag aagcaaagtg aaattttaat
     1681 aaaaatatta taaaaatata ctgtatgtat ataattgata ataagattta tatgattttt
     1741 tacaggaaat atatctgatt tgtataaaaa gttaaagaaa ataatttggt aatttaaaaa
     1801 aatggtttta tatttgtgag attttctgga cgggtaagt
//
LOCUS       DAOSOW010000377         1836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_63819_length_1836_cov_2.695115, whole genome shotgun sequence.
ACCESSION   DAOSOW010000377 DAOSOW010000000
VERSION     DAOSOW010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63819_length_1836_cov_2.695115"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PLD67_08595"
     CDS             <1..752
                     /locus_tag="PLD67_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT22621.1"
                     /translation="LTKKFGGLTAVDNVTFNIEEGEIFGLIGPNGAGKTTIFNLITGI
                     YSITSGEIFYYDKKIENLKPFQIANMGITRTFQNIRLFKKLTAYDNVLTACHKLADYN
                     LFDSVIRFRKFKPQEKFINEKTAELLKLMGLWDYKDIVASNLPYGLQRKLEIARALAL
                     EPKLLLLDEPAAGMNPEETIQLMGLIKEIRDEFKLTVLVIEHHMDLVMGVCDRIFVLN
                     FGIPLALGTPKEIQKDKRVIEAYLGKEEGHA"
     gene            745..1464
                     /locus_tag="PLD67_08600"
     CDS             745..1464
                     /locus_tag="PLD67_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT22622.1"
                     /translation="MLSVKNLNVFYGGIHALKGVTIDVQEGQIVSIIGSNGAGKSTLI
                     NAISGIVKYKDGEIIYKDEKLPKQAHKIVKAGICQVPEGRVIFANLTVMENLNMGAFL
                     RNDKDGIQKDLERIFKLFPILKERQKQMAGTLSGGEQQMLAMGRGLMSNPELILLDEP
                     SLGLAPLLINTIFDIIIEIKKMGKTILLVEQNAFKALSVADKAYVLEQGAIVMEGPAS
                     DLIKDERIQEAYLGKKKSDVG"
     gene            1573..>1836
                     /locus_tag="PLD67_08605"
     CDS             1573..>1836
                     /locus_tag="PLD67_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT22623.1"
                     /translation="MKLTFLGHSALLIEEGNFKGLVDPFITGNSLSKTSVDDLVNITH
                     IFVTHGHGDHIGDTINIAKRNKALIITNHEISAYLSKFKLRTHA"
BASE COUNT          648 a          249 c          383 g          556 t
ORIGIN      
        1 atttaacaaa gaaatttgga gggttaactg ctgtagacaa tgtgaccttt aatattgaag
       61 aaggagagat ttttggttta ataggcccca acggtgccgg caaaactacc atctttaatt
      121 taataacagg aatttacagc attacaagcg gagaaatttt ctattacgac aaaaagattg
      181 aaaatcttaa accttttcaa atagcaaata tgggaattac aaggacattt caaaacatca
      241 gactttttaa aaagctgact gcttatgata atgtgcttac tgcatgtcat aaactggcgg
      301 attataatct tttcgattct gtaataaggt ttagaaaatt taaacctcag gaaaaattca
      361 taaatgaaaa gacagctgag ctgttgaagt taatgggttt atgggactac aaagatattg
      421 ttgcatcaaa tcttccatat ggtctgcaaa gaaagcttga gattgcaaga gccttggcat
      481 tggagcctaa gcttttattg cttgatgagc ccgcagcagg tatgaatcct gaagaaacaa
      541 tacagctgat gggactgatt aaggaaataa gagacgaatt taagcttact gtgctcgtaa
      601 ttgagcatca tatggatctg gtaatgggtg tttgcgatag gatatttgtc cttaatttcg
      661 gcataccttt ggctctcggg actccgaagg aaattcaaaa agataagaga gttattgaag
      721 cttatctagg gaaggaggag ggtcatgctt agtgttaaaa atctaaatgt tttttatggc
      781 ggaattcatg ctctcaaggg agtcaccata gatgttcagg aaggtcagat agtttcgatt
      841 ataggctcta acggagcagg aaagtctacc cttatcaatg caatcagcgg gattgtaaaa
      901 tataaggatg gtgaaattat ttacaaggat gaaaagcttc ctaaacaggc tcataaaata
      961 gtaaaagcag gaatttgtca agttcctgaa ggaagggtta tttttgcaaa tctcaccgta
     1021 atggaaaatt taaatatggg tgctttttta agaaatgata aagacggtat tcaaaaggat
     1081 ttggaaagaa tatttaaact attccccata ttaaaggaaa gacaaaaaca aatggcagga
     1141 actctttcgg gaggagagca gcagatgctt gcaatgggaa gaggtcttat gtcaaatcct
     1201 gagctcatcc ttcttgatga accttcatta gggcttgcac ctttattgat aaatactata
     1261 tttgatatta taattgaaat caaaaagatg ggaaaaacca ttcttttggt agagcaaaat
     1321 gcatttaagg cattgtctgt tgccgataag gcatatgttt tggagcaagg tgcaatagta
     1381 atggaaggac ctgcttcaga tttaattaaa gatgaaagaa tacaggaagc atatttagga
     1441 aagaaaaaat cagatgtcgg ttagccaaat taagtgtcat ttttggaaat ctcatgtgag
     1501 atttcttttt ttagttgatt ttattataat ataaagatat tatatactta ataataaaaa
     1561 agaggtgatt atatgaagct tactttcttg ggacattcgg cattattaat tgaggaagga
     1621 aatttcaagg gacttgtgga cccctttata acaggaaatt ctctgagtaa aacaagtgtt
     1681 gatgatttag ttaatataac tcatattttt gtaactcatg ggcacggtga tcatatagga
     1741 gacacaatta atatagcgaa aagaaataaa gcattgataa ttactaatca tgaaatttca
     1801 gcttatttga gcaagtttaa attaagaact catgca
//
LOCUS       DAOSOW010000378         1831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_64067_length_1831_cov_3.225788, whole genome shotgun sequence.
ACCESSION   DAOSOW010000378 DAOSOW010000000
VERSION     DAOSOW010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64067_length_1831_cov_3.225788"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1509)
                     /locus_tag="PLD67_08610"
     CDS             complement(<1..1509)
                     /locus_tag="PLD67_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOT22624.1"
                     /translation="MNNYRKSSRLGDILLSKKEITRVQLDKALAEQAESGKRLGETLI
                     SLGYISENTLLNTLSEQLGINIVNLDNYPISVDATRLISEKLAIRTNAIPLKITRDKL
                     TVAMSEPLNIFDIEDIEMESGKKAEVVLASKSQITNAIEKYMSSRSTEIAAQDFDKES
                     LKNEDFENLVEEIDLNVSNSPVVRLINSLIRQALKMGASDIHIEPFEKKLRVRVRIDG
                     ELQEILTPSKHTQSAIVTRVKIIAGMNIAEKRLPQDGRIEFKIDNTTVDLRISVMPTV
                     YGEKVVMRLLDRSNFLKSKGELGFTEHNMEMFNSILDSPNGVILVTGPTGSGKTTTLY
                     AALNELNTMNKNIITVEDPVEYKIEGINQVQVNTKAGLLFSTGLRSILRQDPDIIMVG
                     EIRDEETAEIAVRAAITGHLVISTLHTNDAPSTIMRLIDMGVKPFLVAASLRGIVAQR
                     LVKRICTNCKTAHEATEAEKKLLEVTGDTTIYKGRGCPKCFYTGYSGRIAIHE"
BASE COUNT          411 a          394 c          311 g          715 t
ORIGIN      
        1 ttcatgtatt gcaatccttc cgctgtaacc cgtataaaaa catttcgggc agcctcttcc
       61 tttgtaaatg gttgtatctc cggttacttc caacaatttc ttttccgctt ctgtcgcttc
      121 gtgtgctgtc ttacagtttg tacaaattct ttttacaagt ctttgagcta ctatacctct
      181 taaggatgca gctaccagga atggttttac ccccatatct ataagtcgca taattgtcga
      241 aggtgcatcg tttgtatgga gggtagatat tacaaggtgc cccgttatag ctgctcttac
      301 ggctatttcc gccgtttctt catctctgat ttctccaacc attattatgt ccgggtcctg
      361 tctcagtatt gacctgagtc ctgtcgagaa taacaagcct gcctttgtgt ttacctggac
      421 ttggtttatt ccttctattt tgtattcgac cgggtcttct accgttatta tgtttttgtt
      481 cattgtgtta agctcgttta atgctgcata aagagttgtt gtttttccgc ttcccgtagg
      541 accggtaacc aggattactc cgttgggcga atctaatatg gaattgaaca tttccatatt
      601 gtgctccgtg aagcctaatt ctccctttga ttttaggaag ttgcttctgt ccaaaagcct
      661 cattacaact ttttcgccgt agacggtggg cattaccgat atacgcaaat ccaccgtggt
      721 gttgtctatt ttaaattcga ttctgccgtc ctggggaagt cttttttctg caatattcat
      781 tcccgctatt attttaaccc ttgttactat ggccgactgc gtatgctttg aaggtgttag
      841 aatttcctgc agttctccgt ctatacgaac tcttaccctc agtttttttt caaagggctc
      901 tatatgtatg tcgctggctc ccatcttcaa agcctgtctg ataagggagt ttatcagcct
      961 tacaacaggt gaattgctta catttaagtc aatctcttcg actaagtttt caaagtcttc
     1021 atttttcaag gattctttat cgaaatcctg ggcagctatt tcagtgctcc tgctgctcat
     1081 atatttttct attgcatttg ttatttggct tttggaagct aatactactt cagccttttt
     1141 gcccgattcc atctctatat cttctatgtc aaatatattg agtggctcgc tcatggctac
     1201 agtaagctta tcccttgtta ttttcagagg tatggcattt gtccttattg ccaatttttc
     1261 gctgattaaa cgggttgcat ccacacttat gggataattg tccagattta ctatattaat
     1321 gcccaattgc tcagacaagg tgttaagtaa tgtattttca ctgatatatc ctaagcttat
     1381 caatgtttct ccaagtcttt tgccgctttc cgcctgttct gcaagggcct tgtccaattg
     1441 tactcttgta atttcctttt ttgaaaggag aatatcacct aaccttgaag atttcctgta
     1501 attattcatt atttctcctt gtttatgtat ttttttccgg actgtgtttt cttaagacct
     1561 gtccgcatcc tatataatac gcaggattcg tctttatttt tatgttgcct tcattatcaa
     1621 ttgtcaatat taattataat ttgttgtaat tacaattcta ttctttatga attatattta
     1681 aattatattt caattacatt ttaattacgc cgtatcctaa tacaataaaa atccgcaatt
     1741 gcggattttt tatacctcat tttcttattt tttcctctgc ttgttcttag gtacaacaca
     1801 tgtgccgctg gctctgcaaa caactacggg c
//
LOCUS       DAOSOW010000379         1820 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_64601_length_1820_cov_2.206232, whole genome shotgun sequence.
ACCESSION   DAOSOW010000379 DAOSOW010000000
VERSION     DAOSOW010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64601_length_1820_cov_2.206232"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            479..1153
                     /locus_tag="PLD67_08615"
     CDS             479..1153
                     /locus_tag="PLD67_08615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT22625.1"
                     /translation="MEKENMLKAEHETQLLASRMEIHESTLTEISREIHDNINLSLTL
                     AKLQLNTITWNNIEQSKFIVVSCADLIGKSIDELSNLSRSLNADIIASQGLLNALEAE
                     VERVNRSAKINIVMYVFGETKFLESQKELILFRIIQEALQNIIKHAGAVTVQFHLYFH
                     DKELLIHISDDGIGYNAQQSKQSGAGITNMQARVKALNGSLSIEGIQHKGTTITIQIP
                     YSSNDR"
     gene            1143..1808
                     /locus_tag="PLD67_08620"
     CDS             1143..1808
                     /locus_tag="PLD67_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOT22626.1"
                     /translation="MTDKQRKIKVLIADDHVLLRNALAGLIDQFQTCTVIFQACNGLE
                     AMKCLKSGNIPDIILLDLNMPEMDGFETAEQLQMQYPAVKVMALTMYDSELSLIRLLQ
                     FGVKGFLRKDIQPSELKVAIETLHRDGYYHSNYLGSKLSGLFIKGKDNKRKIDEALLQ
                     ENELLFLRYASTDKTYKEIALEMDLSPRAIDSLRDQLFVKLDVKSRVGLAIYAVKNGI
                     VRF"
BASE COUNT          588 a          346 c          330 g          556 t
ORIGIN      
        1 tgaagcaatt tttatttata gttacatgct cgatattctt attgttaata tatactaata
       61 tcaaaagccg tgacattgct tttactaccc ttgttagagt atcacccctc attattataa
      121 ttattggctg catattttac ttttataatt tattaaaaga aacttattca ggcaatccta
      181 taaaattttc tcatttttgg attgtagcag gcgcaagctt tcgcttctta ctaagctttc
      241 caacttcgtt ttctcatcaa atattgacag gtaaaaattt aatgttagat actataagta
      301 tctattcaat tgagctttca aatttagtac tttacttatg cattttaaaa gcttttctat
      361 gcaagaacag caattaagaa taaccatttt ctatattttt acactagtat ttctcatact
      421 gatggcatca gttatagcat tattattgta ttcttataga aaaaagcagg cattacactt
      481 ggaaaaagaa aacatgctca aagctgaaca tgaaacgcag cttctcgcca gccgtatgga
      541 aattcatgaa tcaaccttaa ctgaaatatc ccgtgaaatt catgacaata tcaatctctc
      601 tctaacactt gccaagctgc agctgaacac aattacatgg aataatatag aacaaagcaa
      661 attcattgtt gtttcctgtg ccgacctgat cggtaaaagc attgatgagt tgagcaacct
      721 ttcccggagt cttaatgcag atatcattgc atcgcaaggc ctgcttaatg ccctcgaagc
      781 agaggttgaa agagttaacc gttctgcaaa aatcaatata gttatgtatg tgtttggaga
      841 aacaaaattt cttgaatcgc aaaaggaact gatccttttt cgcatcatac aggaagcttt
      901 acaaaatatc atcaagcatg ccggtgcagt tactgttcag tttcatcttt actttcatga
      961 taaagagctg ctaatccata tttccgatga cgggattggt tataatgcac aacagtcaaa
     1021 acaaagcggt gcaggcatta ccaatatgca agcaagggtg aaagcattaa acggcagtct
     1081 cagtatagaa ggtatacagc ataagggaac gactattacc attcaaattc cgtattcaag
     1141 caatgacaga taaacagcgg aaaataaaag tactgatagc agacgaccat gtactgcttc
     1201 gcaatgcgct tgccggccta attgatcaat ttcaaacatg cactgttata ttccaggcct
     1261 gtaacgggtt ggaagcaatg aaatgtctca aaagcggaaa tattcctgat atcatcctgc
     1321 tcgacttaaa catgcccgaa atggatggat ttgaaacggc agagcaattg cagatgcaat
     1381 accctgctgt taaagtaatg gccctaacaa tgtatgattc tgagctttca ctgatccgct
     1441 tacttcaatt tggtgttaag ggttttctgc ggaaggatat tcagccctca gagttgaagg
     1501 tagccattga aacactgcac agggatgggt attatcattc caattatctt ggcagcaaac
     1561 tttccgggtt gtttattaag ggaaaggaca ataaacgcaa aattgacgaa gcccttttac
     1621 aggaaaacga attgcttttt ctccgttatg cctctacaga taaaacctat aaggagattg
     1681 ccctagagat ggacctaagt ccccgtgcca ttgacagttt aagagaccag ctatttgtaa
     1741 aattagatgt caaaagcagg gtgggcttgg caatctatgc cgttaaaaat gggattgtaa
     1801 gattttaacc ccttactttt
//
LOCUS       DAOSOW010000380         1818 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_64695_length_1818_cov_3.170732, whole genome shotgun sequence.
ACCESSION   DAOSOW010000380 DAOSOW010000000
VERSION     DAOSOW010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64695_length_1818_cov_3.170732"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PLD67_08625"
     CDS             <1..756
                     /locus_tag="PLD67_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT22627.1"
                     /translation="QISIIFQDPMTSLNPVFTIGNQLMEAILLHTNRNKEEAKARAIE
                     MLKLVGVNEPEKRIEQYPHEFSGGMRQRVMIAMALACEPDILIADEPTTALDVTIQAQ
                     ILDLMKELQKKLGMAIILITHDLGVVAEMCDDIIVMYAGQISERGTADDIFYNPKHEY
                     TKGLLKSIPTVQDEKQRLKPIGGNPVDLLNMPAGCPFAPRCDNAMKICLTVNPVEMTI
                     EGKHTSRCWMNVREGIENETLTIKQVEEMYHGK"
     gene            746..1714
                     /locus_tag="PLD67_08630"
     CDS             746..1714
                     /locus_tag="PLD67_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT22628.1"
                     /translation="MENNNNLLEVRNLKQYFDIPVGLLKSKPLKAVDDVSFSIRKGET
                     LGLVGESGCGKTTVGRSILQLYKPTGGEIIFKGKKIESKESIKQLRQNAQMVFQDPYS
                     SLNPRMTVGDIVGEPLDVMNMYKSRKERDKRVNELMEIVGLSSEHATRYAHEFSGGQR
                     QRIGIARALAVEPEFIVCDEPVSALDVSIQAQIINKFEDLQEQLGLTYLFIAHDLLVV
                     KHISNRIAVMYLGRIMELAPSDELYNHPLHPYTISLMSAVPIPDPSYARSHQRILLEG
                     DVPSPLNMPSGCPFRTRCKRATKECAEIRPELREVAPEHLVACIHV"
BASE COUNT          642 a          277 c          388 g          511 t
ORIGIN      
        1 caaataagta ttatttttca ggaccctatg acaagcttga atcctgtatt tactatagga
       61 aatcagttaa tggaagcaat tttattgcat acaaacagaa ataaagaaga agctaaagct
      121 agagcaattg aaatgcttaa attggtcggt gtaaatgaac cggaaaaaag aatagagcaa
      181 tatcctcacg aattttcggg aggtatgaga caaagagtta tgattgcaat ggctcttgcc
      241 tgtgaacctg atattttgat agcagatgag cctacaacgg ctttagatgt taccattcag
      301 gcacaaatac ttgatttgat gaaagagctg caaaagaaat taggtatggc aatcatcctg
      361 attacccatg acttaggtgt agtggcagaa atgtgtgatg atattatcgt aatgtatgcc
      421 ggacaaatat cggaaagagg aacagcagac gatatttttt acaatccaaa gcatgaatat
      481 actaagggat tgttaaaatc catacctacg gtacaagatg aaaaacagcg ccttaagcca
      541 atcggcggca atccggtaga tttattaaac atgccggcag gatgtccatt tgcaccaaga
      601 tgtgacaatg caatgaaaat ttgtcttact gtaaatcccg ttgagatgac aatagaaggc
      661 aaacatactt ccaggtgctg gatgaatgta agagaaggaa ttgaaaatga aactcttaca
      721 ataaaacaag tagaggagat gtatcatgga aaataacaac aatcttcttg aagttagaaa
      781 tttaaaacag tatttcgata ttccggtcgg cttgttaaag agcaaacctt taaaagcagt
      841 tgatgatgtc tcattcagta ttagaaaagg cgaaacctta ggtttggttg gtgaatccgg
      901 ttgcggcaaa acaacagtgg gaaggtctat attgcagttg tacaaaccaa ctggaggaga
      961 aataattttt aaaggcaaaa agattgaaag taaagagtct ataaaacaat taagacaaaa
     1021 tgcacaaatg gtatttcagg atccatattc ttcactaaac ccgagaatga cagtcgggga
     1081 tattgtggga gagcccttgg atgttatgaa tatgtataag agcagaaaag aaagagacaa
     1141 aagagtaaat gaattaatgg aaattgtagg actgtcaagc gagcatgcta ctcgttatgc
     1201 tcatgaattt tctggagggc agcgtcaaag aataggtatt gcaagagcct tggctgttga
     1261 gcctgaattt atagtgtgtg atgagcccgt atctgctctg gatgtttcaa ttcaagcgca
     1321 aattatcaat aaatttgaag atttacaaga acaattggga ctgacttatc tctttatcgc
     1381 tcatgattta ttggttgtaa aacatatatc caatagaatt gcagttatgt atttaggtag
     1441 aataatggaa ttagcgcctt ccgatgaatt gtataatcac ccgcttcatc catatacaat
     1501 ttcgttaatg tcggcagtac ctattcctga cccgagctat gcaagaagcc atcagaggat
     1561 tttacttgaa ggtgatgtac caagtcctct gaatatgcct tccggatgtc cttttagaac
     1621 aagatgtaaa agggcaacaa aagaatgcgc tgaaatcagg cctgaattaa gagaagtggc
     1681 acctgaacat ttagttgctt gtattcatgt ttagaggttt agggacacat aaatgtcccc
     1741 actttgggtg atattaattt aaaattaata tatatttata tttaaaggag gaaagtaatg
     1801 aaaaaatttt tatcttta
//
LOCUS       DAOSOW010000381         1814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_64882_length_1814_cov_3.606026, whole genome shotgun sequence.
ACCESSION   DAOSOW010000381 DAOSOW010000000
VERSION     DAOSOW010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64882_length_1814_cov_3.606026"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..1322)
                     /gene="der"
                     /locus_tag="PLD67_08635"
     CDS             complement(3..1322)
                     /gene="der"
                     /locus_tag="PLD67_08635"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOT22629.1"
                     /translation="MGRPIVTIVGRPNVGKSTLFNRIAGKRISITEDTPGVTRDRIYA
                     ECEWLDNYFTLIDTGGIEPNTTDTIFAQMRIQAEVAIEMADVVLFMVDGKEGVTAADT
                     EVAQMLRKAKKEIILVVNKMDRFVNEDSVYEFYNLGLGDPIPISSAEALGIGDLLDEI
                     ISKFNGKMDTKEEEDSIKIAVIGKPNAGKSSIINRIIGEERVIVSDIPGTTRDAIDTK
                     FERNSKKYTLIDTAGIRKKSKVLESVEKYSVLRAFTAVERADICVLVIDADKGVTEQD
                     IRIAGYSHDSNKGMVIVINKWDLIEKDNSTFKEFTEEIRQKLAYATYAPILTVSALTG
                     QRVNKILEVVDEIYSFRNLRISTGVLNDILTEAVLMNQPATVKGKRLKIYYGTQVAVN
                     PPKFLIFVNDKEIVHFSYERYIENKIREAFEYKGTPIIIEFRNRSEK"
     gene            complement(1336..>1814)
                     /locus_tag="PLD67_08640"
     CDS             complement(1336..>1814)
                     /locus_tag="PLD67_08640"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOT22630.1"
                     /translation="SKYDFPPYEDYEDFDQLENGIGMCRLFKNRVEEEIKNIRYNKVL
                     KDEVTFVTGVAAYELMVELAEKIMSRINVKINVLRIINNYFGDKITVSGLITGKDIID
                     QLKGKNYKNLILPRNMINDNHVMLDDMVIEDLERELNTKVEIYDAEDDTLLSLIVK"
BASE COUNT          475 a          388 c          244 g          707 t
ORIGIN      
        1 tcttatttct cgcttctgtt tctaaattca ataataatag gtgttccctt gtattcgaaa
       61 gcttccctga ttttattttc aatatatctt tcgtatgaaa aatgaacaat ttccttatca
      121 tttacaaata ttaaaaactt aggaggattt actgcaacct gcgttccgta gtatatttta
      181 agtctcttcc cttttactgt tgccggttga ttcataagaa ctgcttctgt aagaatatcg
      241 tttaatactc ccgtagaaat tcttaaattt ctgaagctgt atatttcatc aactacttca
      301 agtattttgt tgaccctctg acctgtaaga gctgagactg ttaatatggg ggcatatgtg
      361 gcatatgcta atttttgtct tatctcttca gtaaattcct taaaagtgct attatccttt
      421 tcaattaaat cccacttgtt tatgacgata accattcctt tgttgctgtc atgagaatat
      481 ccggctattc ttatatcctg ctctgttact cctttatcgg catcaattac caatacacat
      541 atatctgctc tttccactgc agtaaaagct cttaatacgc tgtacttttc gactgattcc
      601 aaaaccttgc tctttttcct tatgcctgca gtatcaataa gcgtatactt cttggagttt
      661 ctctcgaact ttgtatcgat ggcatccctt gtcgtacccg gaatatcact tacgattact
      721 cgctcttcgc ctattattct gttgattatt gaagatttgc ctgcattagg ctttccgata
      781 acagcaattt ttatgctgtc ttcctcttct tttgtatcca tttttccatt gaattttgaa
      841 ataatttcat ccagcaaatc acctatgccc aaggcttcgg cagatgatat cggaataggg
      901 tctccaagcc ctaaattgta aaattcatat acgctgtctt catttacgaa tctgtccatt
      961 ttattgacta cgagaattat ttcttttttt gcttttctga gcatttgtgc cacctcagtg
     1021 tcagcagcgg taacaccttc tttgccgtct accataaaca aaacaacatc cgccatttct
     1081 atggctactt ccgcttggat gcgcatctgt gcaaaaatag tatccgttgt gtttggctct
     1141 attcctcctg tgtctattaa agtaaagtaa ttatcaagcc attcgcattc agcataaatt
     1201 ctgtctcttg taacaccggg agtatcttcc gttatggata ttctctttcc ggcaatccta
     1261 ttaaaaagtg ttgactttcc tacattcggc ctgccaacaa ttgttactat aggtcttccc
     1321 atttatttcc tccgtttatt taacgattaa gctgagcaaa gtatcatctt ctgcatcgta
     1381 tatttctacc ttagtgttta attctctttc caaatcttca attaccatat catccagcat
     1441 cacatgattg tcattaatca tatttctagg caatataagg tttttataat ttttaccctt
     1501 gagttggtct atgatgtctt ttcccgtaat aagacctgat acggttattt tgtctccaaa
     1561 ataattattt ataattctaa gaacattaat tttcacattt attctgctca taattttttc
     1621 ggctaattct accattaatt cataagcagc aactcctgtt acaaaagtaa cttcatcttt
     1681 taatacttta ttatatctta tattttttat ctcttcttct acacggtttt taaataacct
     1741 gcacatacct atcccgtttt ctaattgatc aaaatcctca taatcttcgt agggaggaaa
     1801 atcatattta ctta
//
LOCUS       DAOSOW010000382         1813 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_64955_length_1813_cov_2.343003, whole genome shotgun sequence.
ACCESSION   DAOSOW010000382 DAOSOW010000000
VERSION     DAOSOW010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64955_length_1813_cov_2.343003"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PLD67_08645"
     CDS             <1..361
                     /locus_tag="PLD67_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013931777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOT22631.1"
                     /translation="GYLKILTSKFIEEYRNRIINIHPSLIPAFCGKGYYGIKVHEEVI
                     KYGVKLSGATVHFVDEEADTGPIIIQEAVEVGYEDTAETLQQKVLKIEHKILPLAVKY
                     YCEGKIQISGRKVKIKE"
     gene            363..>1813
                     /gene="purH"
                     /locus_tag="PLD67_08650"
     CDS             363..>1813
                     /gene="purH"
                     /locus_tag="PLD67_08650"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004033726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOT22632.1"
                     /translation="MMRALISVSDKTGIVEFAKELERLGVEIISTGGTHKKLNEAGIK
                     VRGISEVTNFPECLDGRVKTLHPNIHAGLLAMRSNPEHMKQLLDLNIKPIDLVVVNLY
                     PFKQTVLKEGVSREEAIENIDIGGPTMLRSAAKNYQDVAVIVDPKDYELVLNELKENK
                     EVSLDTKFYLCSKVFSHTAHYDTLIASYIKRERKDNTLPDTYTMTFEKIQEMRYGENP
                     HQKAAFYKEVKNVDGLLASALQLHGKELSFNNINDTNGALELLKEFDEPCVVACKHAN
                     PCGVGCGDNIYEAYMKAFNADPTSIFGGIVVFNREIDERTAQEINKIFVEIIVAPSYS
                     QEALSVLMSKKNIRLLQIENILQKQDEYSYDIKKVAGGILVQEIDSKLLPEGEELKTV
                     TFSKPTEKEMKDLLMAWKIVKHTKSNAIVIVKDGQPLGIGPGQVNRIWACKQAIEHAH
                     EFLGKDSTKGASLASDAFFPFPDCAQEAAKAGI"
BASE COUNT          659 a          289 c          380 g          485 t
ORIGIN      
        1 aggatatcta aaaatactaa cttctaaatt tattgaagag tataggaata gaataattaa
       61 tattcatccg tctttaatac cggcattttg cgggaaagga tattacggta ttaaggttca
      121 tgaagaagtt attaaatacg gcgttaaatt aagcggtgca acagtacatt ttgtagatga
      181 ggaagcagac acgggtccaa ttatcattca agaagcggtt gaagtaggct atgaagatac
      241 tgccgaaaca ttacagcaaa aagtacttaa aatagagcat aaaatacttc ctttagctgt
      301 gaagtattac tgcgaaggga aaattcaaat aagcggaaga aaagtaaaaa ttaaggagta
      361 aaatgatgag agctttaatt agtgtatccg ataaaacagg aatagtcgaa tttgctaaag
      421 aattagaaag attaggagtc gagattatat caacgggagg cactcacaaa aaattaaatg
      481 aggcaggaat taaagtaaga ggaatttccg aagtaacaaa ttttcctgaa tgcctcgacg
      541 gaagggtaaa aacgttgcac ccgaacattc atgcaggatt actggcaatg cgctcaaacc
      601 ctgagcatat gaaacagcta ttagatttaa atattaagcc aatagacctg gtggttgtaa
      661 atttgtatcc ctttaaacaa actgttttaa aagaaggcgt aagcagggaa gaagcaattg
      721 aaaatattga tataggcggt cctacgatgc ttcgctccgc agctaaaaac tatcaggatg
      781 tggctgttat agttgaccct aaggattatg aattggttct taatgaatta aaagaaaata
      841 aagaagtttc cttagacact aaattttatc tttgctcaaa ggttttttct catactgctc
      901 attatgacac attgattgca tcttatatta aaagagaaag aaaggacaat actttgcctg
      961 atacatacac aatgactttt gaaaaaatcc aggaaatgcg atatggtgaa aatcctcacc
     1021 aaaaagcagc attttataaa gaagttaaaa atgttgacgg tcttttggct tctgctttac
     1081 agcttcacgg caaggagttg tcttttaata atataaatga taccaacgga gctttggaac
     1141 tcttaaaaga atttgacgag ccttgtgtcg ttgcatgcaa gcatgcaaat ccatgcggag
     1201 taggatgcgg ggacaatatt tatgaagctt acatgaaggc attcaatgca gaccccactt
     1261 caatatttgg aggcatagtt gtatttaaca gggaaattga tgaaaggact gcacaggaaa
     1321 tcaataaaat ctttgttgaa ataattgttg ctccttcata cagccaagaa gctttgtcag
     1381 tacttatgtc aaagaaaaat ataagacttt tgcaaataga aaatatatta caaaagcagg
     1441 atgaatattc ctacgatatt aaaaaggttg ccggcggcat attggttcag gaaattgatt
     1501 caaagctcct tcctgaaggt gaagagttaa aaacagtaac tttctccaaa ccgaccgaaa
     1561 aggaaatgaa ggacttgctg atggcctgga aaattgtaaa gcatacaaaa tccaacgcca
     1621 tagtcattgt taaagacgga cagccccttg gaattggtcc ggggcaggta aaccgtatat
     1681 gggcatgcaa acaggcaatt gagcatgcac atgaattttt gggaaaagat agcacaaagg
     1741 gagcttccct tgcatcggat gcatttttcc cattccccga ttgtgcacaa gaagcggcaa
     1801 aagccggaat aag
//
LOCUS       DAOSOW010000383         1812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_64978_length_1812_cov_3.161639, whole genome shotgun sequence.
ACCESSION   DAOSOW010000383 DAOSOW010000000
VERSION     DAOSOW010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64978_length_1812_cov_3.161639"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="PLD67_08655"
     CDS             complement(<1..372)
                     /locus_tag="PLD67_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOT22633.1"
                     /translation="MSTKLFTFKGGIHPPQSKSFTQNVPIEDAPAPDIVVIPLSQHIG
                     APCDPLVAVGDKVKVGQKIGESKAFVSAPVHSSVSGTVKKIENRQVPGGAKVNCVIIE
                     SDGKYEVHESVQPKGKLEDLSK"
     gene            complement(529..1080)
                     /locus_tag="PLD67_08660"
     CDS             complement(529..1080)
                     /locus_tag="PLD67_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOT22634.1"
                     /translation="MKEISMHILDIAMNSIKADASLIEINIEDSIKNNRLSITIADNG
                     RGMSEDVINRVSNPFYTTRTTRKVGLGIPMLKEACERCNGYLKIESKIGVGTKVCCYF
                     ERDNIDRAPLGNMGETIMTIINSGDNFELTYSHKTDRGEFIFDTREVKEMLEGININD
                     IGVLMWIKEYINENVNLVSAGKI"
     gene            complement(1222..>1812)
                     /locus_tag="PLD67_08665"
     CDS             complement(1222..>1812)
                     /locus_tag="PLD67_08665"
                     /inference="COORDINATES: protein motif:HMM:NF024586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOT22635.1"
                     /translation="DVENLNGSVIVINTNDKILYINDAAKEIFGENYQGLVGKDLKLI
                     WNDYFNYKEDLINNFGTFELKNIIKNNPEIFGEQNIYNSKDEITGTLEKLLINASQYT
                     IEDIFSIVKKYDGVMIPAHINKMTNGILGVLGFMPINLKIETVEVYSKMPINSKIAEK
                     YKVIYNSDAHQLVDIAEAVNYFDLDDIEEIYEYLFE"
BASE COUNT          547 a          360 c          237 g          668 t
ORIGIN      
        1 tttagaaaga tcttctaatt tgccttttgg ctgaactgat tcatgcactt catatttccc
       61 gtcggattct ataataacgc aatttacctt cgctccgccc ggcacctgtc gattttcaat
      121 cttttttact gttccggaca cgctcgaatg aacgggtgct gaaacaaagg cttttgattc
      181 gccaattttt tgtccaactt ttactttatc tcctacagcc accaaaggat cacatggggc
      241 tccaatatgt tgcgacaatg gaattacaac aatatcagga gccggtgcgt cttcaattgg
      301 cacattctgc gtaaaagact tactttgagg gggatgaatt ccacccttaa acgtaaacaa
      361 ttttgtgctc attataccac ctcgtgaaat taataattat gaattataaa aaagttataa
      421 tctcaaacat aaagattata acagattgtt ttcgactttt aaataccttt ttttatttat
      481 ttttataatt ttaacattat ttatcaaatt atgcaagacc ataaattatc aaattttacc
      541 tgccgataca aggtttacat tttcattgat atattcctta atccacatta aaacaccgat
      601 gtcgttaata ttaatacctt caagcatttc ttttacttct ctcgtatcaa aaataaattc
      661 tcccctgtca gtcttatgag aataagttag ttcaaaatta tcaccggagt taataattgt
      721 cataattgtt tcccccatgt taccgagggg tgctctgtct atattatccc tttcaaaata
      781 acagcaaacc tttgttccga cgcctatctt tgattcaatt tttaaatagc cgttacatct
      841 ttcgcatgct tctttcagca ttggaattcc taatcctact tttcttgtag tgcgagttgt
      901 atagaaggga ttagatactc tgtttataac atcttcgctc attcctctgc cgttatccgc
      961 aatagttata cttaaccggt tattttttat actatcttct atatttattt caattaatga
     1021 agcatcagcc ttaatgctgt tcattgcaat atccaatatg tgcattgaaa tttctttcat
     1081 aattcctcca accaagatat tacgcagtgg aatgcaattg gggacattcc tctgaccgac
     1141 ataggttatc ccaatagaaa gaggtgtgtc cccatacctc tataggtgtg tcccccctcc
     1201 ttttagtaaa tacgtggaca ttcattcaaa taaatactca taaatctctt cgatatcatc
     1261 cagatcaaaa taattaaccg cttcagctat atcaacaagc tgatgggcgt ccgaattata
     1321 aatcacctta tatttttctg ctattttact gttaataggc atttttgaat atacttccac
     1381 agtctctatt ttcagattga taggcataaa acctaatacc ccgagaatac cgtttgtcat
     1441 tttgttaata tgggcaggaa tcatcactcc atcatatttc ttgacaatgg aaaaaatatc
     1501 ttcgatggta tattgcgaag cattaatcaa taatttttcc aaggtgcctg ttatttcatc
     1561 cttagaatta tatatatttt gctcaccgaa tatttcagga ttatttttta taatgttttt
     1621 taattcaaaa gtaccaaaat tatttattaa atcttcctta tagttgaagt aatcattcca
     1681 aattaatttt aaatctttac ctactaagcc ttgataattt tctccaaata tttcctttgc
     1741 agcatcattt atataaagta ttttatcatt ggtattaata acaattacag aaccatttaa
     1801 attttcaaca tc
//
LOCUS       DAOSOW010000384         1797 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_65741_length_1797_cov_2.463261, whole genome shotgun sequence.
ACCESSION   DAOSOW010000384 DAOSOW010000000
VERSION     DAOSOW010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65741_length_1797_cov_2.463261"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(440..790)
                     /locus_tag="PLD67_08670"
     CDS             complement(440..790)
                     /locus_tag="PLD67_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22636.1"
                     /translation="MFNEICIYEVKLDKQEEVEALMKEVAEFYKEQQGVIDVKYIKRT
                     HRQKDFNAVKSGEAPIQLTRYVGKVTYLLYWVVENEEVHAKVSKLGLEKFYKRWNRCL
                     ITMPKIFLGENIVE"
     gene            complement(1118..1687)
                     /locus_tag="PLD67_08675"
     CDS             complement(1118..1687)
                     /locus_tag="PLD67_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOT22637.1"
                     /translation="MFNKLIKLETQRLLMRPFSSDDKELIFNVMKDKEMFEYTPDEPW
                     HSIENAEEFINLVLWLYDSEYETFKHFFAVIEKESGKLIGVCGVGGIDYDRSENEVLY
                     HIGKEYWGKGYATEAAQAMLEYSFEQLGLSKVIGVVHPDNNASNRVMEKIGLKRIGTI
                     SGLDEEHAYFNGEYLYSMSIGEYHKKYKS"
BASE COUNT          528 a          438 c          252 g          579 t
ORIGIN      
        1 tacgatcact atttcaagag ttaacaacgc ataaaagtaa attgcttttg ttgatatttt
       61 accactactt cttttcagga ttaatccgat taaaataaga tgtaaaatcg gtaataggcc
      121 caacaaataa agaactgcaa taaaaatgtt cacgacatac ctctttatca cttaatttta
      181 ttacacaata ttcagccgat atgcaatata agaacactcc ttcttacatt aacaatctgt
      241 accatctgtc gcctaacgtc ttcacactca cgccgtccct gaaccggacc cacagagctt
      301 ttccataata tcatactaca ccgtttccgg taccagggac ctgacatgtg ccgcttaaat
      361 aaatattgtc tctattgggt tcaaatctct gacaaactcc gcataggagt ttgaacctta
      421 taagaaaact gtgaaataat tactccacaa tgttttcccc aagaaatatt ttaggcatgg
      481 taattaaaca acgattccat cttttataaa atttctctaa tcctagtttt gagacctttg
      541 catgcacttc ctcattttca accacccaat ataataaata agtaacctta cctacataac
      601 gtgtcaattg aataggtgct tcgccggatt tcactgcatt aaaatccttt tgccgatgag
      661 ttcgtttgat atatttcaca tcaataacac cttgctgttc tttgtaaaat tcagctactt
      721 ctttcataag tgcttcaact tcttcttgtt tgtcaagctt cacttcatat atacaaattt
      781 cattaaacat ataatcaacc tccatacttc ttcatattcc tgtgatatat aatataagaa
      841 taggactact tacacaaacc aaactgcacc atctgccgta taacggttcc gcactcacga
      901 caccactgaa ccggacccca gagcctgtcc cccttgcgga gcttcgcccc cggtgaagag
      961 gtatggttgc tacttaagtc aaacaaaagt attgcatatc gttgcaaccc aaggcacggc
     1021 tactcctcgc tctttccctc ccgtgccact gcttgagggc attgttatgt gaagtcaggt
     1081 gcccagcgga gccagaggcc accatggacg gtggtcccta agatttatac ttcttgtgat
     1141 attctcctat gctcattgaa tataagtatt ctccattgaa gtaagcatgt tcctcgtcca
     1201 gtccgcttat ggtgcctatt ctttttagcc caatcttctc cattactctg tttgaagcat
     1261 tattatctgg atgtactaca ccgataacct tactcaaacc tagctgttca aaagaatact
     1321 ctagcatggc ttgtgctgct tctgttgcat agcctttacc ccaatattcc ttccctatat
     1381 ggtataaaac ttcgttttca cttcgatcat aatcaattcc accaacacca caaacaccaa
     1441 ttagcttccc gctttctttc tcaataacag caaaaaagtg tttgaatgtt tcatattctg
     1501 aatcatataa ccacaaaact agattaataa attcttcagc attttcaatt gaatgccatg
     1561 gttcatctgg ggtatattca aacatttcct tgtccttcat gacattaaaa attaattctt
     1621 tgtcatccga agaaaaaggt ctcattaata atctctgagt ctccaatttt attaatttat
     1681 taaacattac taccgctcct ttacttgcct agaattcctt cgaagcccct cttacctaaa
     1741 tccgggcatg gacgcacgga atctttcatc tgatttcgta taacgggttc gcactca
//
LOCUS       DAOSOW010000385         1794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_65883_length_1794_cov_3.253594, whole genome shotgun sequence.
ACCESSION   DAOSOW010000385 DAOSOW010000000
VERSION     DAOSOW010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65883_length_1794_cov_3.253594"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            322..>1794
                     /locus_tag="PLD67_08680"
     CDS             322..>1794
                     /locus_tag="PLD67_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOT22638.1"
                     /translation="MENLKLKDFLDYTFLSGLELSPDKNYGAFVVHTSDFEDNKYLSN
                     IWLYNLTTSEYKKLTNMNEEKSFIWLDNNTILFSSLRDEKLKKRIQDGEHWTVFYAID
                     IQGGEAYEYMRIPMKVNNIKKIADEKFLLTAIYDHYGINLHSRKGEEKTKAIELIKEN
                     KDYEILDEIPFWSNGEGFINKKRNRLYLFDKGTKDIVPISNQYENIKVTSVKDGKALY
                     VSSIYTNKMELTTGLYMYDLYSSKALCLVEDLEYNIEFAEFVGDDIIVAATTMDKYGI
                     NENFNFYKLQDGELVLLSKHDFGLNNSVNSDCRYGEGAARRVFDNCLYFISTENKSSF
                     IKKLSLDGSIEKLTEDNGSVDCFDICSQGIIFIGLRGSRLQEIYTLQKIKETQRTKFN
                     EKIVEIKTIVKPESINFQNDGVTIEGFVLKPVNFDEKKCYPGILNIHGGPKTVYGEVF
                     YHEMRVHPQNDYSRPGWLRNEQFGAGMITGWGAHHLDTAHW"
BASE COUNT          662 a          259 c          338 g          535 t
ORIGIN      
        1 aaagaaaata ttaaattttc cattataagt cctttcatat tctgtaataa taatgaatct
       61 gtgtagtcat gctcctgaca ttatatatca ggcatttcta atttttgaat acagcatgtc
      121 ctattaatgt tcatatacat gacttttcag tctattataa ttatactctc aaaacaattc
      181 taaaacaata gttttaattg cataactatt ttttaaaata tataatatca ttataacaag
      241 caatatccgt aaacagcttt taaagaaatg catgttcttg ttgtcccgga ttatcatgta
      301 aaagtattat aggaggcaga aatggaaaat ttaaaactaa aggatttttt ggattatact
      361 tttttatccg gacttgaact gtcaccggat aaaaattacg gtgcttttgt ggtacatacg
      421 tcagattttg aggataacaa gtatttgtcc aatatatggt tgtataattt gaccacctcg
      481 gaatataaaa agcttacaaa tatgaatgaa gaaaagagtt ttatttggct tgacaataat
      541 actattttgt tttcttcatt gagagatgaa aaattgaaaa aaagaattca ggacggcgag
      601 cattggactg tattctatgc cattgatatc caaggcgggg aagcttatga atatatgcgt
      661 attcccatga aggtaaacaa tattaaaaaa attgccgacg agaaatttct attaacagca
      721 atttatgacc attacggcat taaccttcat tctcgcaaag gagaagaaaa gactaaggca
      781 attgaattaa taaaagaaaa caaggactat gaaatattgg atgaaatacc gttttggtca
      841 aacggcgaag gatttataaa caagaaaaga aaccgtctat atctgtttga caaaggaaca
      901 aaggatattg taccaatttc aaatcaatat gaaaatataa aagtaacaag cgtaaaagac
      961 ggtaaagctt tatatgtttc ttcaatttac accaataaaa tggaattgac aacaggatta
     1021 tacatgtacg atttatatag cagtaaagct ctttgccttg ttgaggattt agaatataat
     1081 atagagtttg ccgaatttgt aggggatgac ataatagttg cagctactac catggataaa
     1141 tacgggataa atgaaaactt caatttttat aagctacaag atggggaatt ggtacttctc
     1201 agcaaacacg atttcggttt gaacaattcg gttaactcgg actgcagata tggagaagga
     1261 gctgcaagaa gagtattcga taactgcctg tacttcatat ccacagaaaa taaaagttcc
     1321 tttataaaga agctttcatt ggatggaagt attgaaaaat taacggaaga taatggatca
     1381 gtggactgct tcgacatatg cagccaaggt attatcttca ttgggctcag gggcagcagg
     1441 ctgcaggaaa tttatacgtt acaaaaaata aaagaaactc aaagaactaa atttaatgaa
     1501 aagatagtgg aaataaagac gattgtaaaa cctgaaagta ttaattttca aaacgatgga
     1561 gttaccattg aaggatttgt attaaaacct gttaattttg atgaaaagaa gtgctatccg
     1621 ggaatcttaa acatacacgg aggtccaaaa acagtatatg gtgaagtttt ctatcatgaa
     1681 atgagggtac atcctcagaa tgattattca cggccgggat ggctcaggaa cgagcagttc
     1741 ggtgccggaa tgataaccgg ctggggcgca catcacctgg ataccgctca ctgg
//
LOCUS       DAOSOW010000386         1786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_66306_length_1786_cov_3.444830, whole genome shotgun sequence.
ACCESSION   DAOSOW010000386 DAOSOW010000000
VERSION     DAOSOW010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66306_length_1786_cov_3.444830"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /gene="ylqF"
                     /locus_tag="PLD67_08685"
     CDS             <1..623
                     /gene="ylqF"
                     /locus_tag="PLD67_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOT22639.1"
                     /translation="YRSKGWSVVLYNSTDNKELRKLNKAIAEATQEIVERYKKRGLLN
                     KTIKAMIVGIPNVGKSTLINSLARKKSAKTGNMPGVTKGKQWIHLAGNIDLLDTPGIL
                     WPKFDDEECALNLAFTGAIKDDVLVLEEIALKFVERMQQMGKENLILERYGIEAGENP
                     LDILNNIAHKKGFLINKTDIDYHRVARLLFNDFRNGKLGNITLEMP"
     gene            752..1384
                     /locus_tag="PLD67_08690"
     CDS             752..1384
                     /locus_tag="PLD67_08690"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOT22640.1"
                     /translation="MLKIEQGLWDKGYNYIACIDEVGRGCLAGSVVTCAIIMPKGLLI
                     EGVNDSKKLTAKKREELFDIIIESSLGYGIGEYDCKKVDEYNIKNATKLAMVQAVENI
                     KDKEGNKIVPDYILVDAEKLDINIPQFNIIHGDAICHGIAAASIIAKVTRDRQMELLH
                     IQYPEYNFSKNKGYGTKEHIEALLEYGPSEIHRKSFVKKILDKNQQLSFL"
     gene            1585..>1786
                     /locus_tag="PLD67_08695"
     CDS             1585..>1786
                     /locus_tag="PLD67_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22641.1"
                     /translation="MYQKSIVHYMTKICIDILFIVAIICCILVPFSSSWLFQYFGIVG
                     ANTIFIKAILLASGISCAYILWQ"
BASE COUNT          672 a          244 c          361 g          509 t
ORIGIN      
        1 actacagaag caagggctgg tcggttgtgc tttataattc aacagacaat aaagagcttc
       61 ggaaactgaa taaggcaata gcagaagcaa ctcaagaaat tgttgaaaga tataaaaaaa
      121 ggggattatt gaacaaaaca atcaaagcaa tgattgtcgg cataccaaat gtaggcaaat
      181 caacactaat aaattcattg gcaagaaaga agagcgcaaa gacgggaaat atgcccggtg
      241 taacaaaggg caaacaatgg atacacctgg ccggtaatat tgatttattg gatactcccg
      301 gaatattgtg gccgaaattt gatgatgaag agtgtgcttt aaatcttgca ttcacgggtg
      361 caataaagga tgatgtattg gtattggagg aaattgctct taagtttgtc gaaagaatgc
      421 agcaaatggg aaaggaaaat ttaattcttg aaagatacgg catagaagca ggggaaaacc
      481 ctttggatat tttgaacaat atagcccata aaaaaggctt tttaataaat aaaacagaca
      541 tagactacca tagagtagca agattattat ttaacgactt cagaaacgga aaattaggaa
      601 acataacatt agagatgccc taaccccatt tttctgattg tattcagaaa aatggatggt
      661 aggtcattcg caacgaattc ccaaaatatg cgagcaaagg gagcaaatat tttggtgaat
      721 gagactgcga gatgccctaa aggagaataa catgcttaaa attgaacaag gtctgtggga
      781 caagggatat aattacatag catgcataga tgaagtaggg agaggctgtt tagcaggcag
      841 cgttgtaacc tgtgcaatta ttatgcccaa gggtttactg atagaaggcg ttaatgattc
      901 caaaaaactg acggctaaaa aaagagaaga attatttgat ataattattg aatcatcttt
      961 gggatacggt attggagaat acgactgtaa aaaggttgat gaatataata ttaaaaatgc
     1021 aacaaagctt gcaatggtac aagctgtcga aaacataaaa gacaaagaag gaaacaaaat
     1081 agttcctgat tatattcttg ttgatgccga aaagcttgac ataaatatac cgcagtttaa
     1141 tataatccac ggagatgcaa tttgtcacgg aatagcagca gcatcaataa ttgcaaaagt
     1201 aacccgtgac cgacaaatgg agctcttaca tattcaatat ccggaatata attttagtaa
     1261 aaataagggg tatggtacaa aagagcacat tgaagctctg cttgaatacg gtccttccga
     1321 aatacataga aaatcatttg ttaaaaaaat tcttgataag aaccagcagc tgagtttcct
     1381 ataatatcgg cgacgttttt tatttaagta aaaatgaagt tattggaatg aattagaaat
     1441 acagatagaa atataaggca ttgagagtca cgatgcctta tttttattta tcaataaaat
     1501 attatcgtaa aacaataaat atttattgac atgcaaaaaa tatctgctat aattagaata
     1561 caataataaa ttttggagga ttttatgtac cagaaaagca tagttcatta tatgactaaa
     1621 atttgcattg atattttgtt tatagtggca attatttgct gtattttggt tccattttct
     1681 tcgtcttggc tgtttcagta ttttggaata gttggggcaa atactatttt cattaaagca
     1741 atattattag cttcaggtat ttcttgtgca tatattttat ggcagt
//
LOCUS       DAOSOW010000387         1782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_66523_length_1782_cov_2.976838, whole genome shotgun sequence.
ACCESSION   DAOSOW010000387 DAOSOW010000000
VERSION     DAOSOW010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66523_length_1782_cov_2.976838"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1234
                     /locus_tag="PLD67_08700"
     CDS             233..1234
                     /locus_tag="PLD67_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HOT22642.1"
                     /translation="MGGSILKITNEQLKEMYLTMLKIRKFEKSASELFAAGKIPGFVH
                     LYIGEEAVATGACANLREDDYITSTHRGHGHIIAKGGELKYMMAELFGREDGYCKGKG
                     GSMHIADATKGILGANGIVGAGHNIAVGSGLAIQHKKTDQVCVCFFGDASTNQSTFHE
                     ALNLASIWKLPVIFVCENNGYGISVSQKRHQAIADVSVRASAYNMPGITVDGNDLFAV
                     YEAVSEAVARARKGQGPTLIECKTYRWRGHFEGDPVVYRPEGELEAWIKKDPIPKVEK
                     YLLDNGVMNEEEMKKMNDDVDKMVEEAIEFAEKSPFPSLESALKDVYSDIVEEGRVR"
     gene            1231..>1782
                     /locus_tag="PLD67_08705"
     CDS             1231..>1782
                     /locus_tag="PLD67_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOT22643.1"
                     /translation="MKKMTYAEGIREAMRFKMREDPNVILFGEDVGKFGGCFGVSAGL
                     WEEFGDDRIRDTPISEGVIVGAAVGSAACGIKPIAEIMFIDFITVGMDQLVNQAAKMR
                     YMFGGEINLPMVIRVPAGAGIGAAAQHSQSLEGWLTHVPGLKVVYPSNARDAYGLLLS
                     SIDDQNPVVFIENKVLYAMQNDVD"
BASE COUNT          565 a          303 c          464 g          450 t
ORIGIN      
        1 tttctcatca tgacataatt tcaggcatgc attttatatc atttgactgt cctacaatcc
       61 gtgtatgaca aagacctaga atcttaaaag cttgtcataa aaattataat ataaaatatt
      121 atttatatta aaggggtggt gccttggaaa aatttattgt tgaaatcggc atgggagcgg
      181 atttccatgg ccaggatgtg acggatgagt gtatccgtaa aaattaaaaa tgatgggagg
      241 tagtattttg aaaatcacta acgaacaatt gaaagaaatg tatttgacca tgctaaagat
      301 tcgaaagttt gaaaaaagtg cgtcagaatt gtttgctgca ggaaaaattc cgggctttgt
      361 ccacctctat ataggtgaag aagctgttgc aacaggcgct tgtgcaaatt taagggaaga
      421 tgactacata accagcaccc acagagggca cggacacata atcgccaagg gcggagaatt
      481 aaaatacatg atggctgaac tttttggaag ggaagacgga tattgcaaag gcaaaggcgg
      541 ctcaatgcat attgcggatg caaccaaggg cattttggga gcaaacggta ttgtaggcgc
      601 aggtcataat attgcagtag gttcgggctt ggctattcaa cacaagaaaa cagaccaggt
      661 ctgcgtatgc tttttcggtg atgcgtcaac caaccagagt acattccatg aagctttgaa
      721 cttggcaagt atttggaaac tcccggtaat atttgtgtgt gaaaacaacg ggtatggtat
      781 ttctgtaagc cagaaaagac accaagctat tgcggatgtt tccgtgagag catctgcata
      841 taacatgccc ggtattactg tagacggaaa tgatttgttt gcggtatatg aggctgtgag
      901 cgaagcagtt gcaagagcca gaaaaggaca aggtccaacc ctcattgagt gcaaaacata
      961 caggtggaga ggtcattttg aaggagaccc ggttgtgtac aggccggaag gagaattgga
     1021 agcatggatt aagaaagatc caattcccaa ggtagaaaaa tacttgctgg ataacggtgt
     1081 tatgaatgaa gaggaaatga agaaaatgaa tgacgatgtg gacaagatgg ttgaagaggc
     1141 aatagagttt gccgaaaaga gccccttccc atcattggaa tcagctttaa aggatgtgta
     1201 ttctgatata gttgaggagg gaagagtaag atgaaaaaga tgacatatgc cgaaggaata
     1261 agggaagcaa tgcgttttaa aatgcgggaa gatcccaatg ttatactgtt tggagaagat
     1321 gtgggtaaat tcggaggatg cttcggagtc agtgccggct tgtgggaaga gttcggggat
     1381 gacagaattc gggacacccc catatcggaa ggtgtaatag taggtgctgc cgtaggctca
     1441 gccgcatgcg gaataaaacc catcgctgaa ataatgttca tagattttat cacggttggt
     1501 atggaccaat tggttaacca ggcagccaaa atgaggtaca tgttcggcgg agaaataaac
     1561 ctccccatgg ttataagagt accggcagga gcaggcattg gtgctgcagc ccagcactcc
     1621 cagtccctgg agggatggct gacccatgtt ccgggcctta aagttgttta tccgtcaaat
     1681 gcccgggacg catacggttt acttctatca tccattgatg accaaaatcc tgtggtattt
     1741 attgaaaata aagtacttta tgcaatgcaa aacgatgttg at
//
LOCUS       DAOSOW010000388         1775 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_66872_length_1775_cov_3.723256, whole genome shotgun sequence.
ACCESSION   DAOSOW010000388 DAOSOW010000000
VERSION     DAOSOW010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66872_length_1775_cov_3.723256"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLD67_08710"
     CDS             <1..327
                     /locus_tag="PLD67_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT22644.1"
                     /translation="VLSQDYIRTARAKGVKPAKVIFKHALRNAVTPVVTYAGPMFAYI
                     ITGSLVVEKIFAVPGLGKEFVSSIINRDYTMIMGTTIFLGTLIIIMMLVSDILYKVFD
                     PRIELG"
     gene            346..1425
                     /locus_tag="PLD67_08715"
     CDS             346..1425
                     /locus_tag="PLD67_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOT22645.1"
                     /translation="MNNIDEMPKKNPLSFQLNLKDFEKATEDEKAQQVTMSESITFFK
                     DGMRRLRKNKIAMTCLTVLILIALIVTFVPMVYPYTYEQQLGVAQGKRIDKTYNNLKP
                     FEYGETELERIANGEKIFPHIFGTDAAGRDYAIRVIYGGRISLLVGFFAAIIVLIIGV
                     VYGSIAGYFGGKVDLFMMRIVDIIYSLPDMLIIILLSVVLNQVLQPKIEGTSLAKVGT
                     DMISIFIVFALLYWVSMARLVRGQILSIKQNEYILAAKSVGSSPWRIIKKHLLPNSMS
                     VIIISTALQIPSAIFTESYLSFLGLGVSIPMPSLGSLASDALTGIYSYPYRLIIPAIA
                     IWLIVLSFNLLGDGLRDAFDPKLKK"
     gene            1436..>1775
                     /locus_tag="PLD67_08720"
     CDS             1436..>1775
                     /locus_tag="PLD67_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT22646.1"
                     /translation="MSLLQVKDLYTSFFTSSGEVKAVNGVSFNLEEGKVLGIVGESGS
                     GKSVTAYSILQILTNPGRIVSGSIKLKGQELVGCSKETMQKIRGNQISIIFQDPMTSL
                     NPVFTVGNQLM"
BASE COUNT          587 a          258 c          385 g          545 t
ORIGIN      
        1 gtattaagtc aggattatat aagaacggca agggcaaaag gagtaaaacc ggcaaaggta
       61 atattcaaac acgctcttcg aaatgccgta acacctgttg taacatatgc cggtccaatg
      121 tttgcatata ttatcacggg aagtttggtt gtcgaaaaaa tatttgcagt accgggtctt
      181 ggaaaagaat ttgtttcaag cattatcaat cgtgactaca ctatgattat ggggaccaca
      241 atatttttag gtactcttat aattataatg atgcttgttt cggatatttt gtacaaagta
      301 tttgacccga gaatagaatt gggatagaaa gagaggatgt gacatatgaa taatatagat
      361 gaaatgccta aaaagaatcc tcttagtttt caacttaatt taaaggattt tgaaaaagca
      421 acagaagatg aaaaagctca gcaggtaacg atgagcgaaa gtatcacctt ctttaaggat
      481 gggatgagaa ggctaagaaa gaacaaaata gcaatgacat gcttgactgt attgattctg
      541 atagctttaa tagtgacttt cgtacctatg gtgtatccct atacctatga gcagcaatta
      601 ggagtggcac aaggaaaaag aattgataaa acttataata atttaaaacc ctttgaatat
      661 ggagagacag aattggagcg tattgcaaac ggtgaaaaaa tatttcctca tatattcgga
      721 actgatgctg caggaagaga ttatgcaata agagttatat acggcggaag aatttccttg
      781 ctggttggat ttttcgcagc aataatcgta ttgataatag gagtggttta cggctctatt
      841 gcaggttact tcggaggaaa agtagacctt ttcatgatga ggattgttga tattatttat
      901 tctttgccgg acatgcttat tatcattttg ttatcggttg ttttaaatca agtattgcag
      961 cctaagatag aaggtacttc acttgcaaaa gtgggtacgg atatgataag tatatttata
     1021 gtatttgctc ttctgtattg ggttagcatg gcaagacttg tgcgcggtca aattttatca
     1081 attaagcaaa atgaatatat attagcagca aaatcagttg gctccagtcc atggagaatt
     1141 ataaagaagc atttgcttcc aaacagtatg agtgttataa ttatttctac agcattgcag
     1201 attccgtcag ctatatttac ggaaagctac ttgagttttc tcggtttggg tgtttcaata
     1261 cccatgccca gtctaggttc attagcttcg gatgcattga ccgggattta ctcttatcca
     1321 tacagattaa taatacccgc aatagctatt tggttgatag tattgtcctt taacctgttg
     1381 ggtgacgggc ttagagatgc atttgaccca aaattaaaga agtaggagga atgaaatgag
     1441 tttattacaa gtaaaagatt tgtatacttc atttttcact tcttccggcg aggtgaaggc
     1501 tgttaacgga gtatcattta acttagaaga aggcaaagtt ctgggtattg ttggagaatc
     1561 aggctccggg aagagtgtta cagcatattc aatacttcaa attttaacaa atcccggaag
     1621 aattgtaagc ggcagtataa aattaaaagg acaggaattg gttggctgca gcaaagaaac
     1681 tatgcagaaa ataagaggga accaaataag tattattttt caggacccta tgacaagctt
     1741 gaatcctgta tttactgtag gaaatcagtt aatgg
//
LOCUS       DAOSOW010000389         1752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_68099_length_1752_cov_2.658810, whole genome shotgun sequence.
ACCESSION   DAOSOW010000389 DAOSOW010000000
VERSION     DAOSOW010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68099_length_1752_cov_2.658810"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLD67_08725"
     CDS             <1..465
                     /locus_tag="PLD67_08725"
                     /inference="COORDINATES: protein motif:HMM:NF015780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HOT22647.1"
                     /translation="YIDKIKKHPLNRALRIDKFTAAVLESIFHEYIDEKRAVENIPVL
                     KMINMNIEDLKVRAEKLLAKLEDMFEYLDIGIEECLSQIGGGALPLERMKSYAVSIKP
                     KTMSCAKFERELRNSDVPIIGRVVNDTFYIDIRTVLEGEEQIIYDEIKNLFK"
     gene            481..>1752
                     /gene="selB"
                     /locus_tag="PLD67_08730"
     CDS             481..>1752
                     /gene="selB"
                     /locus_tag="PLD67_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HOT22648.1"
                     /translation="MRNIIIGTAGHIDHGKTTLIKALTGRETDRWEEEKRSGITIDLG
                     FTYFDLPDGYKAGIIDVPGHEKFIKNMLAGVVGMDMVLLVIAADEGIMPQTTEHINIL
                     NLLGVENGIVVITKCDIVDEEWISLVREDITETISSTFLEGAPIVEVSSKTGQGIEKL
                     IQEIINIADKAVKERELNTIPRLPIDRVFSIQGFGTVITGTLITGILKKGEEVEIYPV
                     NKICRIRNIQVHSSDVEKAYAGQRTAINLSNIKKNEIYRGCVIAPVNSMKNTMMLDVK
                     LNLLKSSKRVVINRSRLHLYTGTSEILTRVVLLDRDELTPGESCYAQLRLEEEIAVRR
                     GDKFIVRFYSPLETIGGGEIIEPLPLKRKRFDENLIEELKIKEKGTGADVIEKIIKET
                     KDLLSVSGLAKTTALTETEVQDNIEILEQEEK"
BASE COUNT          669 a          237 c          378 g          468 t
ORIGIN      
        1 tatatagata aaataaaaaa acatccttta aacagggcct tgagaatcga caaatttacg
       61 gcggcagtat tggaaagtat atttcatgag tatattgacg aaaaaagagc agtagaaaac
      121 atccctgtat taaaaatgat aaacatgaat atcgaagact taaaagtacg tgcagaaaag
      181 cttttggcta agcttgaaga tatgtttgag tatttggata ttggaataga agaatgtttg
      241 tctcaaatag gagggggagc acttccttta gaaagaatga aaagctatgc tgtttccata
      301 aaaccaaaaa caatgagctg tgcaaagttt gaaagagaat taagaaattc tgatgttccg
      361 attataggaa gagttgttaa tgatactttc tatatagata taagaactgt attagaaggt
      421 gaagagcaga taatttatga cgagattaaa aatttattca aataatactc tggaggtatt
      481 atgagaaata ttattattgg aacagcagga catatagacc atggtaaaac cacattaata
      541 aaagcattga caggaagaga aacagacaga tgggaagaag aaaagagaag tggaataacc
      601 attgatttag gatttactta ttttgacctt cccgatggtt acaaggccgg tataatagat
      661 gtgccgggtc atgagaaatt tattaaaaac atgcttgccg gagtagtggg aatggacatg
      721 gtgcttttgg ttattgcagc cgatgaagga attatgccgc agaccacaga gcatataaat
      781 attttaaact tgttaggcgt ggaaaatgga attgttgtta ttactaaatg cgatattgtt
      841 gacgaagaat ggataagcct cgttagagaa gatataactg aaaccattag ctccacattt
      901 ttagaagggg caccgatagt tgaagtatca tccaagacag gtcaaggcat agaaaagctt
      961 attcaagaaa tcattaatat tgccgataag gcagtaaagg aaagagaatt aaacacaatt
     1021 ccaaggcttc ccatagacag agttttttcc atacaaggtt ttggcactgt aataacagga
     1081 actttgatta caggcatact aaaaaaggga gaagaagtag aaatttatcc tgttaataaa
     1141 atttgcagga taagaaatat tcaggttcat tcctctgatg tggaaaaggc ttatgccggt
     1201 caaagaaccg caataaactt gtcaaatatt aaaaagaatg aaatttacag gggatgtgtt
     1261 attgctcctg ttaattcaat gaaaaatacc atgatgttgg atgttaagct taatttgcta
     1321 aaaagctcca agcgagtggt aataaaccgt tcaagacttc acctttacac cggtaccagc
     1381 gaaattctta cacgggtagt gcttctcgac agagatgaat taacaccggg agaaagctgc
     1441 tatgctcagc tcagattaga agaagaaatt gccgtgagaa ggggagacaa atttattgta
     1501 aggttttatt ctcctttaga aacaatcgga ggaggagaaa ttatcgagcc tttgccttta
     1561 aaaagaaaaa gatttgatga gaaccttatc gaagagctta aaattaaaga aaagggaaca
     1621 ggtgccgatg ttatagaaaa aataattaag gaaacaaagg atttactctc tgtttcgggc
     1681 ttggcaaaaa caactgcatt gacagaaaca gaagttcaag acaatattga aatattggaa
     1741 caggaagaaa aa
//
LOCUS       DAOSOW010000390         1745 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_68523_length_1745_cov_3.294083, whole genome shotgun sequence.
ACCESSION   DAOSOW010000390 DAOSOW010000000
VERSION     DAOSOW010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68523_length_1745_cov_3.294083"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..1376
                     /locus_tag="PLD67_08735"
     CDS             93..1376
                     /locus_tag="PLD67_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004616131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOT22649.1"
                     /translation="MIIHVVQPGETIYSIANQYNISPQKIIIDNELENPNVLVPGQTL
                     VILIPDVIHVVQPGETLADIARQYNTTTVNLLQLNPFIEYADQIVPGDQITISNTADK
                     RGLIHVLGYAYPHIDRTVLLKTLPYLTYLTIFTYGITEEGELIDIEDEDIIRMARDFG
                     VAPLMLISTLTEEGTFSNELAHVILNDPVAQNNLIENVLQNLREKGYYGLDIDFEYVL
                     PEDEEAFINFIQNVHDRISPEGFVLFTALAPKTSADQPGLLYEAHNYPRIGEASDRVL
                     LMTYEWGYTYGPPMAVSPVDRVREVLDYAVTEIPREKIYMGIPNYGYDFILPYVQGVS
                     RADSVSNVEAVDLAADVGAYIQYDEVSQAPYFIYYDEQGRQHRVWFEDARSILAKLDL
                     FSEYGFEGVGYWNVMRYFPQNWLLVSSLYDIAKLV"
     gene            1452..>1745
                     /locus_tag="PLD67_08740"
     CDS             1452..>1745
                     /locus_tag="PLD67_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22650.1"
                     /translation="MNNKNYRVLKFFEPVNKKDKSRGYVRLDMRGNRGNMIVAAENLG
                     DAKSSSEVYLYKDKHNKIKLGDINNKKGIIKKKLTFENSNAIEDYNVCAVVKDG"
BASE COUNT          608 a          270 c          359 g          508 t
ORIGIN      
        1 atgtgttttt tgtttgttat agcccgacat ttcttcataa caattgaaat tttcatgcat
       61 atactaatga gagaacagaa aggagaatgt ttatgataat tcatgttgta cagcccggag
      121 aaacaatata ttccattgca aatcaataca atatatcacc gcaaaaaata attatagata
      181 acgaacttga aaaccctaat gtccttgtac ccggccagac cttagtaata cttattccgg
      241 atgtaattca tgttgtacag ccgggagaaa cattggcaga tatagcaagg caatacaata
      301 caaccaccgt aaatttactg cagcttaatc cctttattga atatgcagat caaattgtac
      361 ccggtgacca aattacaata agcaatacag cagataaaag aggtctcata catgtgcttg
      421 gatatgctta tccgcatata gacaggacag tacttttgaa aacacttcct tacttaactt
      481 atctgacgat atttacttac ggaatcactg aagaaggcga attaatcgat atagaagacg
      541 aagacataat cagaatggca agggattttg gagtagcacc gcttatgctt atttccacat
      601 taactgaaga agggactttt agcaatgaat tggcacatgt tattttaaat gatccggttg
      661 cacaaaacaa tttaattgaa aatgtattgc agaatttaag agaaaaaggt tattacggac
      721 tagacataga ttttgaatat gtcctgccgg aggacgaaga agcttttata aattttattc
      781 aaaatgttca tgacagaata agccctgaag gatttgtttt atttacggcg ttggcaccaa
      841 aaacatcagc agaccagccg ggacttctct atgaagctca taattatccg agaatcggag
      901 aagcgtcaga tagagttctt cttatgactt atgaatgggg ttatacttat ggtcctccaa
      961 tggctgtatc tcccgttgac agggtaaggg aagtgttaga ttatgcagtg actgaaatcc
     1021 cgagggaaaa aatttatatg ggcattccta actacggtta tgattttata ctgccatatg
     1081 ttcaaggtgt atcaagagcg gactccgttt ccaatgttga ggcagttgat ttggcagctg
     1141 atgttggagc atacattcag tatgatgaag tatcacaagc tccctacttt atttattatg
     1201 atgaacaggg aaggcagcat agagtttggt ttgaagatgc aagaagcatc ttggcaaaat
     1261 tggatttatt cagtgaatac gggtttgaag gtgtcgggta ttggaacgta atgagatatt
     1321 tcccgcagaa ctggctttta gtgagcagtt tatatgatat tgccaagctt gtttgattaa
     1381 agcttgccat atctgtaatt atacaaaaaa atcagcatat aaataaataa cagagatatt
     1441 aggaggtttt tatgaacaat aaaaattata gagtgttgaa gttttttgag ccggtaaata
     1501 aaaaagacaa gtctagaggc tatgtacgtc tggacatgag agggaacagg ggaaacatga
     1561 ttgtagctgc tgaaaattta ggtgatgcaa aatcatcaag tgaagtttat ttatataagg
     1621 ataaacacaa caaaatcaaa ttaggagata ttaacaataa aaaaggtata atcaaaaaga
     1681 agctcacctt tgaaaacagc aatgcaattg aagattacaa cgtatgcgcc gttgtaaagg
     1741 atgga
//
LOCUS       DAOSOW010000391         1742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_68686_length_1742_cov_3.421458, whole genome shotgun sequence.
ACCESSION   DAOSOW010000391 DAOSOW010000000
VERSION     DAOSOW010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68686_length_1742_cov_3.421458"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="PLD67_08745"
     CDS             complement(<1..189)
                     /locus_tag="PLD67_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22651.1"
                     /translation="MDKSLISEIKCIIDEIEGYDYISKVKYVILLICNELYNSINENP
                     MKSTAIFKEYAKFDLENFK"
     gene            303..530
                     /locus_tag="PLD67_08750"
     CDS             303..530
                     /locus_tag="PLD67_08750"
                     /inference="COORDINATES: protein motif:HMM:NF019487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HOT22652.1"
                     /translation="MPIIANQANSKVKLVLNAGLDVNNKTIMKSKTFANVKSAAQNED
                     LYNLGVAISDLQEYELANIVRYDEYELLEEI"
     gene            565..777
                     /locus_tag="PLD67_08755"
     CDS             565..777
                     /locus_tag="PLD67_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HOT22653.1"
                     /translation="MAKKLVMSFLTAEGATSSMTIDAPKDDLTEAEVRAVMELIITQN
                     VFNTSKGDLAEIKSAQIISTTEEVLI"
     gene            993..>1742
                     /gene="dsdA"
                     /locus_tag="PLD67_08760"
     CDS             993..>1742
                     /gene="dsdA"
                     /locus_tag="PLD67_08760"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016103104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HOT22654.1"
                     /translation="MKEEILTKYPSLKNVSELKEVFWLNPKMVPYEEAASKINIDIAA
                     IDDAEMRLKKFAPLIEKVFPETLPSHGIIESPITEINNMKNKLQELYKMNISGKLLLK
                     MDSHLAIAGSVKARGGIYEVLKYAEALAMKHGLLKEDDDYSILAEQKYRDFFGKYKIQ
                     VGSTGNLGLSIGITSAALGFNVTVHMSADAKQWKKDLLKSKGAKVVEYDSDFSKAVEE
                     GRKQSDQDPMSYFVDDENSMNLFLGYSVAARR"
BASE COUNT          633 a          290 c          326 g          493 t
ORIGIN      
        1 cttaaagttt tccaagtcaa acttagcata ttccttgaaa atcgccgtgc ttttcatagg
       61 gttttcattt atcgaattat acaattcatt gcatatcaat aaaattacat acttcacctt
      121 gctaatgtaa tcatatcctt caatttcatc aattatgcac tttatttcac ttataagcga
      181 cttgtccata actacccctt gaattgcatc attatgtata tactagcact attttactat
      241 ttaagcaata catcctgctt ataaatgcta tatagttaca gaataaaacg ggaggtgata
      301 taatgccgat aattgcaaat caagcaaatt ctaaagttaa attagttctt aatgcggggc
      361 ttgacgtaaa caacaagact atcatgaaga gcaagacatt tgctaacgtg aaatcagctg
      421 cgcaaaatga agacctgtac aatcttggag ttgctatttc agaccttcag gaatacgagc
      481 ttgcaaacat tgtaagatac gatgaatacg aattgttaga agaaatataa gttaatataa
      541 atcttaaacg aaaggggtga tataatggct aaaaagttag tgatgtcttt cttgacggcg
      601 gagggagcaa cttcatccat gacgattgat gcaccaaagg atgatttgac agaggcagaa
      661 gtgagagcag tcatggagtt aatcataaca caaaatgttt tcaacacttc caagggcgac
      721 ttggctgaaa taaaatcagc tcaaataatt tcaactaccg aagaagtttt aatataattt
      781 gttagtttat tatgttataa tgaatacaaa taatgaagca tagactttgc tctttgcacc
      841 gatacaatga atacaaacgg ggacattctt taggcatgtc cccatgttaa aaactaagtt
      901 gtcattctga gagtttgccc gaagaatctc aaaacctgaa attccgctgc actcaggatg
      961 acattacata tagaatatac taaggaggaa atatgaaaga agaaattcta actaaatatc
     1021 caagtcttaa aaatgtatct gaattgaaag aagtattctg gctaaacccc aaaatggttc
     1081 cttatgaaga ggctgcatct aaaataaata ttgatattgc agcaattgac gatgctgaaa
     1141 tgaggcttaa aaaatttgcg ccgctgattg aaaaagtatt tcctgaaact cttccatctc
     1201 atggcataat tgaatctccc ataacagaaa tcaacaatat gaaaaacaag cttcaagaac
     1261 tttataaaat gaacatttcc ggaaaactgc tgcttaaaat ggacagtcat cttgcaattg
     1321 caggaagcgt aaaggcaaga ggcggcatat atgaagtatt aaagtatgct gaagcattgg
     1381 caatgaaaca tggattgtta aaagaagatg atgattattc aattttggca gagcagaaat
     1441 acagagactt tttcggcaag tataaaatac aggtcggctc caccggaaat ttaggcttaa
     1501 gtataggcat tacatcggca gccctcggat ttaatgttac agtccacatg tcggcggatg
     1561 caaagcaatg gaaaaaggac ctgcttaaat caaaaggagc aaaggtcgtg gaatatgaca
     1621 gcgatttttc taaagcagtg gaagaaggac gaaaacaatc agaccaagac cctatgagtt
     1681 attttgtaga tgatgaaaac tcaatgaatt tatttttagg ctacagtgtt gcagcaagaa
     1741 gg
//
LOCUS       DAOSOW010000392         1736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_69058_length_1736_cov_3.335515, whole genome shotgun sequence.
ACCESSION   DAOSOW010000392 DAOSOW010000000
VERSION     DAOSOW010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69058_length_1736_cov_3.335515"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..835)
                     /locus_tag="PLD67_08765"
     CDS             complement(284..835)
                     /locus_tag="PLD67_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22655.1"
                     /translation="MDIDIEKIKKARIVLDKMANGISPIDGSRIDEESFLNDPRIIRC
                     LFFVNEILQMTINGELECRNIDRKKLPFVITDEEKTQVVFPEYDIGVNAFSQCVNKVI
                     NPHKSKKLSGAELNKQLKKMGILGEEENEQGKKKTIVPEESVKYGIHTVLSNYNGAEY
                     EKVVFDSNGVNFLLENLEKIMSV"
     gene            complement(970..1692)
                     /locus_tag="PLD67_08770"
     CDS             complement(970..1692)
                     /locus_tag="PLD67_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22656.1"
                     /translation="MKAYKKEASADKEKIFSYIENDMISDYYSIKPKRTYKKSVPVAA
                     IILLIFIAGVRIYNFAGTNIPNASIGITKAISGIGSEAAPVRTASNKKLQELHRYLND
                     IINLKLKQNDITSQYNKSVSDFNNRIITSKGLVLSIEELIRQISIISRESESIKEAEG
                     MEHYHSMFKKSINLQSSLFEAILLKQKTGKAKYNDDINKLKSKIDTLDAGMMDEMITK
                     FDELGIEYHLNSEGGITYTIRY"
BASE COUNT          463 a          367 c          236 g          670 t
ORIGIN      
        1 gttccataat gaagcatgtc tttagatacc atttcgaacc tccaaaatac ttattttata
       61 tctggccaat gatgcaggcc tgatattcaa caaaacaatc ggttttatta aaaatttttc
      121 aacattgtta attttttatc tatccattat tatcataacc tatagacaga tttaagtaaa
      181 ggatattaac ggcaaataaa aaaccctaac ttttcgttag ggaataattc tccttttgcc
      241 tttatatcct ttcactccgg ctaataccat gatattaatt cctttatacg ctcataattt
      301 tttccaggtt ctccaaaagg aaatttactc cgttgctgtc aaacacaacc ttttcatatt
      361 ctgcaccgtt ataattagac aataccgtat gtatgccata ctttaccgat tcctccggta
      421 caatggtttt tttctttcct tgctcgtttt cctcttcccc tagaatgccc atctttttga
      481 gctgcttatt aagctccgct ccgctgagct ttttactttt atgaggatta ataactttat
      541 ttacacattg agagaaagca tttaccccga tatcatattc cggaaacaca acttgtgtct
      601 tttcttcgtc cgtaatgaca aaaggcagtt ttttccgatc gatatttctg cattccaatt
      661 cgccgtttat tgtcatctgc agaatctcat taacaaaaaa gaggcatcga ataatgcgag
      721 ggtcatttaa gaaactttcc tcatcaatcc ttgacccgtc gatagggctt attccgttag
      781 ccattttatc aagcactatc cttgcctttt tgatcttttc tatatcaata tccattcgaa
      841 tcctcccttt cctcacgatt tagcaacaca actgcactta tctcaacgtg ctgttaattt
      901 gttaaatata gtatattgtt ttttatccca taattcaata gctatcattc cctggcacca
      961 tcgggtcttt taatatctta tagtatatgt aatcccacct tctgaattta ggtggtattc
     1021 aattcccagc tcatcaaact ttgttatcat ttcatccatc atcccggcat caagggtgtc
     1081 aattttactt tttaatttgt tgatatcatc attatatttg gctttacctg ttttttgctt
     1141 cagcaatata gcttcaaata atgagctttg caaattaatg ctttttttaa acatgctatg
     1201 gtagtgctcc attccctctg cttcttttat actttctgac tctctgctta taatacttat
     1261 ttgccttatc aactcttcta tacttagaac aaggcccttt gaggttatga tcctattatt
     1321 gaaatcggat actgatttat tatattgtga tgttatatca ttctgcttga gctttaaatt
     1381 gattatatcg ttgagatatc tatgcagctc ctgcaatttt ttattcgatg ctgttcggac
     1441 tggagcagct tcactgccaa taccgcttat tgctttcgta atacctatag aagcatttgg
     1501 gatattggta cctgcaaaat tatatatcct cactccagca ataaatatta gcaggataat
     1561 tgctgccaca ggcacagatt ttttatatgt ccttttgggt tttatcgaat agtaatcaga
     1621 tatcatatca ttttctatat aactgaatat cttctcttta tcagctgaag cctctttctt
     1681 gtatgctttc atttgctttc taaagtttaa ctgtgtcttt gtcggcatag tacccc
//
LOCUS       DAOSOW010000393         1720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_69926_length_1720_cov_4.151952, whole genome shotgun sequence.
ACCESSION   DAOSOW010000393 DAOSOW010000000
VERSION     DAOSOW010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69926_length_1720_cov_4.151952"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..634
                     /locus_tag="PLD67_08775"
     CDS             137..634
                     /locus_tag="PLD67_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOT22657.1"
                     /translation="MSLYKQWTENLEAIKTQGEYNEFFKGYMEKETECYRRILENKTA
                     VIEGKIVELAEENKMSALEYIGFLDGANTSFKKELVLDDLTEESEIKAEFDFEKLLWN
                     MHDAKAEWLYGMKEWENIFDEDKRKQIKKEFNRSKIVVKGEKIGRNDPCPCGSGLKYK
                     KCCGK"
     gene            655..1527
                     /locus_tag="PLD67_08780"
     CDS             655..1527
                     /locus_tag="PLD67_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOT22658.1"
                     /translation="MNKTKGLTFGILAGFIYGFTPILGKLTYLEGSNPISLTFYRNLL
                     SIPFFFVMLKYNNVPLKVTKSQTKQLAILGLLASATALSLYGSYNYISVGMSTTIHYI
                     YPVLVTAACIIIFGEKISRDKVVSLILSTMGITLFFEGSINITGILIAFMSGVFFAGY
                     LLFMDKSGLNTIYQFKITFYTAAFSSMYLFIFGIISKNLVFTMTFKGWFFTVLVAVFV
                     SFFANTFVALGVKYVGPTVTSIVGMLEPITSIVMGILFINEPITLRNILACVLILLGV
                     LIVSLSKEKASEDY"
     gene            complement(1557..>1720)
                     /locus_tag="PLD67_08785"
     CDS             complement(1557..>1720)
                     /locus_tag="PLD67_08785"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOT22659.1"
                     /translation="TGFNITIRDITVSAGAGFLVALTGDIMKMPGLPKVPAAENIDID
                     VDGRIKGLF"
BASE COUNT          593 a          250 c          325 g          552 t
ORIGIN      
        1 cacggcctga agaatctcat cggttaagaa tggcaaaggg gtttgtaaat tgtgcgctct
       61 aaggagcgct tttttttatt gaatttttcc attttcatga taaaataaaa gaacagtata
      121 ttttttggag gaagttatga gtttatataa acaatggaca gaaaacttag aagcaattaa
      181 aacccaggga gaatataacg aattttttaa agggtatatg gagaaagaaa cggaatgtta
      241 cagaagaatt cttgaaaaca aaactgctgt aattgaagga aaaatagttg aattggcaga
      301 agaaaataag atgtccgcat tggaatacat agggttttta gacggtgcaa acacaagttt
      361 caaaaaagaa ttagtcttag atgatttaac tgaagaatct gaaataaaag cagaatttga
      421 ctttgaaaag ctgttatgga atatgcatga tgcaaaagcc gaatggcttt acggaatgaa
      481 ggaatgggaa aatatctttg atgaagataa aagaaaacag ataaaaaagg aattcaatcg
      541 ctccaagata gttgtaaaag gtgaaaaaat aggaagaaat gacccatgcc cctgcggttc
      601 aggattaaaa tataaaaagt gctgcggaaa ataaaaatga tataggaggg aattatgaat
      661 aaaacaaaag gcttaacatt cggtatctta gccggattta tttatggttt cactcctata
      721 ttgggaaaat taacgtattt ggaaggaagc aatcccatat cacttacatt ctatagaaat
      781 cttctttcca ttccgttttt ttttgttatg ttaaaatata ataatgtacc tttaaaagtt
      841 acaaagagcc agacaaaaca attagcaata ttaggattgt tagcttccgc taccgcactc
      901 agcctttacg gctcctataa ttatatatct gttggaatga gcacaactat tcattatatt
      961 tatcctgtat tggttactgc tgcgtgtatt attatttttg gtgaaaaaat aagcagagac
     1021 aaggtagttt ctttgatact atccacaatg ggaattactt tatttttcga aggaagtatc
     1081 aatataacgg gaatactgat agcttttatg tcaggtgtat tttttgcagg atatttatta
     1141 tttatggata aaagcggttt gaatacaata tatcaattta aaataacatt ttatacagcc
     1201 gccttttcat ctatgtacct gtttattttt ggtataataa gcaaaaacct tgtttttaca
     1261 atgacattta agggatggtt ttttacggtc ttagttgcag tctttgtttc attttttgca
     1321 aatacattcg tagccctggg agtaaaatat gtaggtccta cagttacgag tatagttgga
     1381 atgcttgaac ctattacaag catagtgatg ggtatattgt ttataaatga accgataaca
     1441 ttaagaaata tccttgcatg cgtgctgata ttgctcggtg ttctgatagt gtcgctatcg
     1501 aaagaaaaag ctagcgagga ttattaataa agaaggggac attccgtccc ctctcattaa
     1561 aacagtcctt ttatccttcc gtcaacatct atatctatat tttctgctgc aggtacttta
     1621 ggaagccccg gcatcttcat aatatctccc gtcaaggcaa ccaaaaatcc tgctcccgct
     1681 gatactgtta tatctctgat tgttatatta aaacctgtag
//
LOCUS       DAOSOW010000394         1709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_70549_length_1709_cov_3.612455, whole genome shotgun sequence.
ACCESSION   DAOSOW010000394 DAOSOW010000000
VERSION     DAOSOW010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70549_length_1709_cov_3.612455"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="PLD67_08790"
     CDS             complement(<1..807)
                     /locus_tag="PLD67_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOT22660.1"
                     /translation="MESKLNLNPHLIESARIAARNIADDVQKFIDKNTTVTVERTIAR
                     LMGVDGVDEIEKPLPNVLIDSIKEGGGLSRGAAFWIGNAMVNTGKTPQEIAEKVSSGE
                     IEITRLPIADENKIMEAVYETAKQMVKKIKNSRAKRDEYLKKLGEGRKPYLYVIVATG
                     NIYEDVLQAQAAAKQGADVIAVIRTTAQSLLDYVPYGPTTEGFGGTYATQENFRIMRK
                     ALDEVGEEVGRYIRLCNYCSGLCMPEIAAMGAIERLDVMLNDALYGILFRD"
     gene            complement(884..>1709)
                     /locus_tag="PLD67_08795"
     CDS             complement(884..>1709)
                     /locus_tag="PLD67_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOT22661.1"
                     /translation="VSIKEFSHILQKNTEIIGEIDYLIAKANYAQKTCSVEPEITNEL
                     QITIKGGRNLKLEKILKDKHIEYVPIDLNLNKNVSCITGANMGGKTVTLRMIGQIAAM
                     ASFGMFVPCEYARLCLFERIFISAGDNQSIEKGLSTFGAEIVNLKEAIKSSDLRCLIL
                     IDELAGGTNPKEGYAITKAVVNYLKKKDCITVLTTHYDNIANDEDIKNLQVKGLSLPD
                     ETDFSKQDNIDSIQKYMDYRLIEVNYSGDIPKDALRIAKMAGIDEEIIKDAENYLK"
BASE COUNT          429 a          377 c          268 g          635 t
ORIGIN      
        1 atctctgaaa agaattccgt aaagagcatc gttaagcatt acatcaagcc tttctattgc
       61 tcccattgcc gctatttcag gcatgcaaag acctgagcag tagttgcaaa gacgaatata
      121 tcttcctact tcttctccaa cctcatcaag agctttacgc attatcctga agttttcctg
      181 agttgcataa gttccgccga agccctcggt tgtaggtccg taaggaacat agtcaagcaa
      241 gctctgagct gttgttctta taacggcaat tacgtcggca ccttgctttg cagccgcctg
      301 tgcctgcaga acatcctcat aaatattgcc tgttgcaact attacataca aatatggttt
      361 tcttccttca ccgagttttt ttaagtattc atcccttttt gctctgctgt ttttaatctt
      421 tttaaccatt tgctttgcag tttcataaac tgcttccata attttgtttt catcggcaat
      481 tggcagtctt gttatttcaa tttctcccga gcttactttt tctgctattt cctgaggggt
      541 ttttccggta ttaaccatgg catttcctat ccagaatgct gctcctctgg ataatccgcc
      601 gccttcctta atagaatcta tcaatacatt gggaaggggt ttttctattt catcaacgcc
      661 atctaccccc atgagacgtg caatagtccg ttcaactgta acagttgtat ttttatcaat
      721 gaatttttgt acatcgtctg caatatttct tgctgcaatt cgtgcacttt caattaaatg
      781 gggattgaga tttaatttac tttccataca tcctcctctt acggggacat tccttcatgg
      841 tctgacctca atagcttact ctccatgaag agtgtcgccc ttcctatttt agataattct
      901 ctgcatcttt gattatttct tcgtctattc ctgccatttt tgctatcctt aaggcatctt
      961 tcggaatatc accggaataa ttcacttcta taagcctgta atccatatat ttttgaatgc
     1021 tatcgatgtt gtcttgtttg ctaaaatcag tctcatcagg aagactaagc ccttttacct
     1081 gcaaattttt tatgtcttca tcatttgcaa tattgtcata atgagttgta agtactgtta
     1141 tgcaatcctt ttttttaaga taatttacta ctgcctttgt aattgcataa ccttcctttg
     1201 gattagtacc tcccgccagc tcatcaatca aaatgagaca tcttaagtca gagcttttta
     1261 ttgcttcttt tagatttact atttcagctc caaaggtaga aagacctttt tcaattgact
     1321 gattgtcacc ggcagaaata aatatgcgct caaaaagaca taatcttgca tattcacagg
     1381 gtacaaacat accaaagcta gccattgctg caatttggcc tatcatccta agggttacgg
     1441 ttttcccccc catatttgcc cctgttatgc aagacacatt tttattaaga tttaaatcaa
     1501 tgggaacata ttcaatatgt ttatctttca atattttttc aagtttaagg tttctgcctc
     1561 ccttaattgt gatttgcagc tcattagtta tttccggctc tacggagcag gttttttgtg
     1621 cataatttgc ttttgcaata agatagtcga tttcaccgat aatttctgtg ttcttttgca
     1681 atatatggct aaattctttt attgacacc
//
LOCUS       DAOSOW010000395         1704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_70811_length_1704_cov_4.440267, whole genome shotgun sequence.
ACCESSION   DAOSOW010000395 DAOSOW010000000
VERSION     DAOSOW010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70811_length_1704_cov_4.440267"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..646
                     /locus_tag="PLD67_08800"
     CDS             155..646
                     /locus_tag="PLD67_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008517605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HOT22662.1"
                     /translation="MFNLGDKVVYPMHGAGVIETMESKEILGEIKNYYVLKMPIGEMK
                     LMIPVDNVNNIGLRNIIDKHAVEEVYKVLKQKAEINDSNWNKRYRDNMTKMKTGDIFE
                     VAQVVRDLTYRDREKGLSTGEKKMLVSAKQMLISEITLATGMDSEEIQDYMDEIINFD
                     ALE"
     gene            849..>1704
                     /locus_tag="PLD67_08805"
     CDS             849..>1704
                     /locus_tag="PLD67_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HOT22663.1"
                     /translation="MIDKIVKAVIAVIGASLGAVSGVLLNNSGLLELPSLQSTLVIIG
                     ISLVFGIIFFLLSSKIKKTGLKIIEALETEILKFSTMDIVLGSLGLIVGFIIAFLISQ
                     PLSDIKYVGTIISVISFVFFGYLGVKIATRKKDDVLWPQILNLKKSSTVKKQEKNKKE
                     IISPKILDTSVIIDGRISDICKTGFIEGTLVIPEFVLKELRHIADSSDSLKRNRGRRG
                     LDILNILQKDDTVSVVIESKDYDDSIEVDVKLLKLAKELGGKVLTNDFNLNKVAEFQG
                     VEVLNINEL"
BASE COUNT          626 a          210 c          347 g          521 t
ORIGIN      
        1 ctttagtaaa agcttcagaa ataaaagagg gagctttaat aaaatttaaa gtaaaaatta
       61 tttttattgt tgacaagcat gcgctatcac gatataatat tcgatttgac atttgttgcc
      121 taatgaggta taatgtattg ataggaggtg cagcatgttt aatttaggcg acaaagtagt
      181 ctaccccatg cacggagccg gtgtaattga aactatggaa tccaaggaaa tcttaggtga
      241 aattaaaaac tattatgtac tgaaaatgcc cataggggaa atgaaattga tgatacctgt
      301 ggataatgtg aataatatag gtttaagaaa catcatcgat aaacatgcag tagaggaagt
      361 atataaggtt ttaaaacaaa aagcagaaat taatgactct aactggaata agagatatag
      421 ggataacatg acaaaaatga agacgggaga tattttcgaa gttgctcagg ttgtaagaga
      481 tttaacctac agggacaggg aaaaaggcct ttcaaccgga gaaaagaaaa tgttagtaag
      541 tgcaaaacaa atgctcataa gcgagattac gcttgcaacc ggcatggaca gtgaggagat
      601 acaggattat atggacgaaa ttataaactt tgatgcatta gaatagtaaa attgctctaa
      661 tgcatttttt ctgcatcaga atttttggtg catacattta gccggatgtc aaatttaata
      721 gaatattaat gaattcttat tgtattattt cgataatagt tatagccaaa tatcaaaaaa
      781 tatgttacat tataaataat actaaataat ataactttta atggttatac ttgtaaggag
      841 gtgataatat gattgataag attgtaaaag cggttatagc tgttataggt gcatctctgg
      901 gagcagtatc gggtgtattg ttaaataaca gcggtcttct tgaattacca agtttgcaaa
      961 gtacattagt aattataggt attagtttag tttttggaat tatatttttt cttctttctt
     1021 ctaaaattaa aaagaccggt ttgaaaataa tagaagctct tgagaccgaa atattgaaat
     1081 tttccactat ggacatagtg ctgggatcat tagggctgat agtaggattc ataatagcat
     1141 ttcttataag ccagccccta tccgacatta agtatgtagg tactattatt tcagttattt
     1201 catttgtatt tttcggatat ctgggtgtaa agattgctac acgaaaaaaa gacgatgttt
     1261 tatggcctca aatattaaat ttaaaaaaga gctcaacggt taaaaaacaa gagaagaata
     1321 aaaaagaaat aatatctcca aaaattcttg ataccagtgt aattattgac ggaagaatat
     1381 ctgatatttg caaaacagga ttcatagaag gaaccttagt cattccggaa tttgtattga
     1441 aggaactcag acatattgca gattcctctg acagccttaa gcgcaacagg ggaagaagag
     1501 gtttggatat tttgaatatt cttcaaaaag acgatacagt aagtgttgtt attgaatcaa
     1561 aagactacga tgacagtatt gaagtagatg taaagctttt gaagttggca aaggaattag
     1621 gcggcaaggt gctgacaaat gactttaatt taaataaggt cgctgaattt caaggggttg
     1681 aagtgttgaa catcaacgag ctgg
//
LOCUS       DAOSOW010000396         1701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_71001_length_1701_cov_3.401580, whole genome shotgun sequence.
ACCESSION   DAOSOW010000396 DAOSOW010000000
VERSION     DAOSOW010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71001_length_1701_cov_3.401580"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="PLD67_08810"
     CDS             complement(<1..1303)
                     /locus_tag="PLD67_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOT22664.1"
                     /translation="MCSETAHKDEHCRLFFGMICLIFGKKEESMEIKYFDAFIEAMEL
                     INDGIQIIDASGKIIYHNPAAKQLDLIDAEKTIGRHILEVYPSLTFETSTIMNVLKNG
                     KPIYNVEQNFVNYKGDKISTLNSTLPIIYNNKVIGALEISRNITEVRELSERIVNLQK
                     ELYEANKGEKKKSKPLAKYTFLDIIGQNKEILKLKSLALKAAMTDSPVIVAGSTGTGK
                     ELFVQSIHNSGDRKYKPFIAQNCAALPANLLESILFGSVKGSFTGAENRPGLFELADG
                     GTLFLDEINSMPLDLQTKLLRVIQDGRVRRVGSGKTTDVDVRIISAINTDINTVVETH
                     QIRQDLFFRLSVITLVIPDLCKRIEDIPLLVEHFIDKYNKKYNKFFSGVSKEVLHIFL
                     NYSWPGNVRELEHAIESAISLYDGEIIREEHLPFQFKNYQPK"
     gene            complement(1351..>1701)
                     /locus_tag="PLD67_08815"
     CDS             complement(1351..>1701)
                     /locus_tag="PLD67_08815"
                     /inference="COORDINATES: protein motif:HMM:NF020711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HOT22665.1"
                     /translation="ELITEAKLEKLKDCFVVNYDESEITEHKGSRTRLKIYKDKMLMT
                     KVGAFSSKMEFRQGKKYRNLYSTPFGSFDLEYTTVLYNNNLNEKGRGSIHAEYMIAFG
                     DTDKSYNKLNIEIM"
BASE COUNT          481 a          337 c          263 g          620 t
ORIGIN      
        1 tcttcggctg ataattttta aattggaaag gaagatgttc ttctcttata atttcaccgt
       61 catacagcga tatggcgctt tcaatggcat gctcaagctc tcttacattg cccggccagc
      121 tgtaatttaa aaatatatgt aaaacttctt tagaaactcc actgaaaaac ttattgtatt
      181 tcttattata tttatctatg aagtgctcta ccaatagagg tatatcttct attcttttgc
      241 ataaatcagg aatcaccaag gttataacac tgagcctgaa aaataaatcc tgtcttattt
      301 ggtgagtttc aaccactgta ttaatatccg tattaatagc ggatataatt ctaacatcaa
      361 catcagttgt tttcccggag cccactcttc tgacccggcc gtcttggatt actctgagaa
      421 gctttgtttg caggtctaaa ggcatagaat ttatttcatc caaaaataag gttccaccgt
      481 cagctaattc aaataagccc ggcctgtttt ccgccccggt aaaacttccc tttacagagc
      541 cgaacagaat actttcaagg aggttagcag gcagggctgc gcagttttga gcaataaaag
      601 gtttgtattt cctgtctccg gaattatgaa tggactgcac aaataattcc ttgccggttc
      661 cggtgctgcc tgcaactatt acgggggaat ccgtcattgc agcttttaga gcaagagatt
      721 taagtttaag aatttctttg ttctgaccta ttatatccaa aaaagtatat ttagctaatg
      781 gttttgactt ttttttctca cctttatttg cttcatacaa ctctttctgc aggtttacaa
      841 ttctttccga caattctctt acctcagtaa tatttcgtga aatttccaag gctcctataa
      901 ctttattgtt ataaataatg ggaagggttg agtttaatgt agaaatttta tcacctttgt
      961 agttaacaaa attttgttca acattataaa taggtttacc gtttttgagt acattcataa
     1021 tggtactcgt ttcaaaagta agagaaggat atacttcaag tatatgtctt ccgattgttt
     1081 tttcggcatc tattaaatcc aactgttttg ctgcaggatt gtgataaatt atttttccgc
     1141 ttgcatcaat aatttgaata ccatcattta ttaattccat tgcttctata aaagcatcaa
     1201 aatatttaat ttccatactt tcctctttct tgccgaaaat taagcatatc atgccgaaaa
     1261 acaggcggca atgttcgtct ttatgcgcag tctcgctgca catttttgtc tgctccctct
     1321 ggtcgcacaa aacaggcgct cgttgcatct ctacattatt tcaatattta gtttattata
     1381 acttttatca gtatctccga aagctatcat atattcggcg tgtatactgc ctcttccttt
     1441 ctcattcaaa ttattattat ataagaccgt agtgtattcc aaatcaaaac taccgaatgg
     1501 agtagaatat aaattacggt attttttgcc ttgcctgaat tccattttag aagaaaaagc
     1561 tccaaccttt gtcattaaca ttttatcttt atatattttc aatctggtac ggcttccttt
     1621 gtgctccgtt atttcacttt cgtcatagtt aacaacgaaa caatccttta atttctctaa
     1681 ttttgcttct gtaatcagct c
//
LOCUS       DAOSOW010000397         1699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_71152_length_1699_cov_3.006691, whole genome shotgun sequence.
ACCESSION   DAOSOW010000397 DAOSOW010000000
VERSION     DAOSOW010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71152_length_1699_cov_3.006691"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="PLD67_08820"
     CDS             complement(<1..1051)
                     /locus_tag="PLD67_08820"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOT22666.1"
                     /translation="MDKNQQPSYKINHLVSANKLKAYIRIELLDKSEEVEISCDEIFE
                     YLKEQEITYGILESDIIEFCKNKEYSKELIAASGKEPVNGKDAEIIYNFDISNEKKFA
                     EGDDGTIDFRNLNNVTNVKKDTVLCYIVPASEGEDGIDVYGNPISYKKGRDISFNYGK
                     NTYISEDGLQLLASTDGCVEFKNNNVYVEDVYKVNNVDNSTGNINFVGSVIINGDVKE
                     GFSVTAAGDIKIRGMVEGAYIKSDGDVVISKGMNGMSKGSIYAKGNITSKYIENATIA
                     SEKNIYAEALINSDVKAGDSIIIKGLISSIIGGTTAAKNKIYAKTIGNKTNPETNIII
                     DLTEYIEEQKEIEKKI"
     gene            complement(1083..>1699)
                     /locus_tag="PLD67_08825"
     CDS             complement(1083..>1699)
                     /locus_tag="PLD67_08825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HOT22667.1"
                     /translation="TEGFKKSKPEFSDLIYTNMRGTRGADTELKSGSGSKISKIDVVF
                     SQGTPYQTGVKTDFAILGEGFFAIQFEEENLFTRGGSFEITNIDDENYLTYQGGYILN
                     NQEEPIIIKNSDDIDSIEIGVFTFNNNADLEQAGNNLYRISNEDAEFNLAEAKVLKGY
                     LEASNVEISDEMVKLIQIQRAFQLNSTVIKTADEIEQTINTLRG"
BASE COUNT          448 a          350 c          216 g          685 t
ORIGIN      
        1 tgattttttt ttctatttcc ttttgttctt ctatatattc tgttaaatca attattatat
       61 ttgtttcggg atttgtttta ttgcctatag tcttggcgta aattttattt tttgcagcgg
      121 ttgttcctcc gataatagaa gaaatcagtc cttttattat aatagagtct cctgctttta
      181 catcgctgtt tatcagcgct tctgcgtata tatttttttc ggatgctatg gttgcatttt
      241 caatatactt gcttgttata tttcctttcg cataaatgct gcctttgctc atgccattca
      301 tacccttgct gataactacg tcaccgtcag attttatata tgcaccttcc accatacccc
      361 ttattttaat atctcctgca gcagttaccg aaaatccttc ttttacatct ccatttataa
      421 ttacgcttcc tacaaaatta atattgcctg ttgaattatc cacattattt actttataaa
      481 catcttccac atatacatta ttatttttaa attctacaca tccgtcggtg cttgccaaaa
      541 gttgcaggcc gtcttctgaa atatatgtat ttttcccgta attaaatgat atatctcttc
      601 cttttttata gctgatggga tttccatata catctattcc gtcttctccc tctgaggcag
      661 gaactatata gcataaaacc gtatcctttt ttacgtttgt tacattattt aagtttctaa
      721 aatcaatagt cccgtcatca ccttcagcaa actttttttc atttgaaata tcaaaattat
      781 aaattatctc ggcatccttc ccgtttacag gctccttccc gctggcagca ataagctcct
      841 ttgaatattc cttattcttg caaaattcaa ttatatcact ttctaaaatt ccatatgtaa
      901 tttcttgctc ttttaagtat tcaaaaattt cgtcacagga aatttcaact tcctctgact
      961 tatcaagaag ttcaattctt atatatgcct tcagtttgtt tgctgatacc agatgattga
     1021 ttttgtatga gggttgctgg tttttatcca tgactcctcc ttattttgat atttctccaa
     1081 tcttatcccc ttaatgtatt aatggtttgt tcaatttcgt cggcagtttt tataacggta
     1141 gaattaagct gaaatgctct ttgaatttga attaacttta ccatttcatc agatatttca
     1201 acattggaag cttccaggta acccttcaaa acctttgctt cagctaggtt aaactctgca
     1261 tcttcatttg aaattctgta taggttgtta cctgcctgct ctaaatcagc attattgtta
     1321 aaagtaaaaa cccctatctc tatactatct atatcgtcag aatttttaat aattataggc
     1381 tcctcctgat tgtttaatat atatcctcct tggtatgtaa ggtaattttc atcatcaata
     1441 tttgtaattt caaagctgcc gcctcttgta aacaaatttt cctcttcaaa ttgaattgca
     1501 aaaaaacctt caccaagtat tgcgaagtct gttttaacac cggtttgata tggagttccc
     1561 tgactgaaaa caacatcaat cttactgatt ttagacccgc ttcctgattt taattccgtg
     1621 tcggctcctc tagttcctct catattggta taaatcaaat cggaaaactc aggctttgat
     1681 ttcttaaaac cttctgttt
//
LOCUS       DAOSOW010000398         1698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_71229_length_1698_cov_2.339623, whole genome shotgun sequence.
ACCESSION   DAOSOW010000398 DAOSOW010000000
VERSION     DAOSOW010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71229_length_1698_cov_2.339623"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..492
                     /gene="fliN"
                     /locus_tag="PLD67_08830"
     CDS             124..492
                     /gene="fliN"
                     /locus_tag="PLD67_08830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOT22668.1"
                     /translation="MKTNQNQKEKEETFVNVRPVEYESFDDEEEVLSEEQLSNLELIM
                     MVPLTISVEIGKTSKQIKDILEISPGTIIELDKQAGALVDIIVNGKLIAKGEVVVVND
                     NFGVRVVEILKKDELMKITL"
     gene            680..1048
                     /locus_tag="PLD67_08835"
     CDS             680..1048
                     /locus_tag="PLD67_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002577856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOT22669.1"
                     /translation="MEGKILIVDDAAFMRMMIKDTLKSSGYENIIEAADGEQAIQTYK
                     NEKPDLVIMDITMPNKNGLDALKEIRKMDPNAVIVMCSAMGQESMVVEAIRNGAKDFI
                     VKPFKSERIIKTVQSIIDRG"
     gene            1045..1407
                     /locus_tag="PLD67_08840"
     CDS             1045..1407
                     /locus_tag="PLD67_08840"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOT22670.1"
                     /translation="MIIINKDIISLIISIIGIIIVLYLTYYCTKWLSTKTTIAAKSRY
                     MNVADKLMLGPNNFITIVEINNKYYLIGITEKDINIIKELEDFHHLPDDKESSNTFNN
                     ILNKYKKTFIGSNNDKKL"
     gene            1478..>1698
                     /locus_tag="PLD67_08845"
     CDS             1478..>1698
                     /locus_tag="PLD67_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliP"
                     /protein_id="HOT22671.1"
                     /translation="MDINGNSVETLEILFLLTIIALLPSILIMMSSFTRIIIVLSFVK
                     NALGLQQTPPNQVLIGIAIFLTLFTMSPV"
BASE COUNT          697 a          211 c          310 g          480 t
ORIGIN      
        1 tagaattgct ccaataataa taagtggaaa aggaatatta aaaaagaaga tgcagtaaat
       61 aaaaaaattg acacgataat caagcaaaat aatgattatg aagaaataat aaaaagggag
      121 cgtatgaaaa caaatcaaaa tcaaaaggaa aaagaggaaa cattcgtaaa tgtaagacct
      181 gtagaatatg aatcatttga tgacgaagag gaagtattat cagaagagca gctttctaat
      241 ttggaattga taatgatggt tcctttgact atcagtgttg aaatcggaaa aaccagcaaa
      301 caaataaagg atattcttga aatttcaccg ggaacaatca ttgagcttga taagcaggca
      361 ggagcattgg tagatattat tgttaacggt aaattaattg caaaaggtga agttgttgtg
      421 gtaaatgata attttggagt aagagtagtt gaaatattaa aaaaagatga attaatgaaa
      481 ataacattat aattgaaata ttcaaaattg ttatttaaag caaggctttg tcatcctgat
      541 tgcagcgaag gctctcatga gattcttcga gctaaagctc tcagaatgac agtttgtgag
      601 ttattaataa caatgacaaa aacattattc atagaaagac acataagtta catagaaatg
      661 acatacaacg gaggatagaa tggaaggtaa aattttaata gtagatgatg ctgcatttat
      721 gagaatgatg ataaaggaca ccttaaaaag cagcggttat gaaaatataa tagaagcagc
      781 tgacggggag caggcaattc aaacatacaa aaatgaaaaa cctgacttgg taataatgga
      841 tataaccatg cccaataaaa atggattgga tgcattgaag gaaataagga aaatggaccc
      901 aaatgccgta atagtaatgt gctcggcaat gggacaagaa tccatggttg tcgaagcgat
      961 aagaaacggt gctaaggatt ttatagttaa accctttaaa tcggaaagaa ttataaaaac
     1021 cgttcaaagt ataatcgata ggggttgatt ataattaata aagatataat ctcacttatt
     1081 atatcaatta taggaataat aattgttttg tatttgacct actattgtac aaaatggctt
     1141 tcaacaaaga caaccatagc cgctaaatca agatatatga atgtagcaga caaattaatg
     1201 ctgggtccga acaattttat tactatagta gaaattaaca acaagtatta tttaataggc
     1261 attacggaaa aagatatcaa tatcataaaa gaattagaag attttcacca tttacccgat
     1321 gataaagaaa gcagtaatac attcaataat attttgaaca aatataaaaa aacatttata
     1381 ggaagcaaca atgacaagaa gttataataa atcaattttt atagtcttaa tattaatgct
     1441 tatgaatact caggctgttt acggcggagt tattaatatg gatataaacg ggaattcagt
     1501 agaaacattg gaaattctat ttcttttaac aattattgct ttgctcccgt caatcctgat
     1561 aatgatgtca tcttttacaa gaataattat tgtattatca tttgtaaaaa atgcgctggg
     1621 actgcagcag acgccgccta atcaggtact gataggcata gcaatattct taacattatt
     1681 tacaatgtca cccgtaat
//
LOCUS       DAOSOW010000399         1693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_71490_length_1693_cov_3.649573, whole genome shotgun sequence.
ACCESSION   DAOSOW010000399 DAOSOW010000000
VERSION     DAOSOW010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71490_length_1693_cov_3.649573"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="PLD67_08850"
     CDS             complement(<1..1519)
                     /locus_tag="PLD67_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004100105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HOT22672.1"
                     /translation="MIRILKRTGNFVDFNADKISNAIMKAMKETKDGVDEELAKEISL
                     KIEEELSSKNYPIPVETVQDMVENYLMDSVRKDVAKKYILYRYERDKSRDSRKRRDSK
                     LLSEEFISKYKHRGSPMDQLGNFVYYRTYSRWLPEERRREYWWETVRRAVEYNCSLVP
                     TKREEAEQLYDNIFNLRQFLSGRTFWVGGTPVSYNYPMANFNCAFEIINDFHAFRDLF
                     YLLMIGSGVGVRILKSDIEQLPKIRGNYKIIHEDYTPVEKSKREDNTGVEFNQNNSVK
                     ITVGDSKEGWVQSLDYFFSFINSSEYRNINTIIINYNNVRPKGEVLKTFGGTASGHAS
                     MKNMFTKIDSVIKKRGAIEGKDRFKLKPIDCLDIANIIGENVVVGGVRRTAEIMLIDY
                     DDTECIEAKSKLYKQIDGQWIVDKDIIHRSMSNNSIYYRKKPTREQLKWQIEQMRYSG
                     EPGWVNEEAGSKRRPNMSGVNPCGEILLDNKGLCNLTTVNVYAFVDENGNLDERGL"
BASE COUNT          454 a          364 c          239 g          636 t
ORIGIN      
        1 taagccctct ttcatccagg ttcccgtttt catcaacaaa tgcatagaca tttacagttg
       61 ttaaattaca aagtcctttg ttatcaagga gtatttcacc gcagggatta actccgctca
      121 tatttggtct tcttttgctt cctgcctctt cattaaccca tcccggctct ccggaatacc
      181 gcatttgctc aatttgccat tttaactgct cgcgggtagg tttcttcctg taataaattg
      241 aattattgct catagagcgg tgtattatat ccttatcaac aatccactgt ccgtctattt
      301 gtttatagag cttgctttta gcctcaatac attctgtatc gtcataatca ataagcatta
      361 tttcagcagt tcttcttacg ccgccgacta ctacattttc acctataatg tttgcaatat
      421 caaggcagtc gataggtttt aatttaaatc tgtccttacc ttcaattgca ccacgctttt
      481 taataaccga atcgattttt gtaaacatat ttttcatact tgcatgaccg ctggcagttc
      541 ctccaaaagt ttttaacacc tctcccttgg gtctgacatt gttgtagtta attattattg
      601 tattaatatt tctatattcg cttgaattta taaagctgaa gaaataatca agggattgaa
      661 cccatccttc cttgctgtcg ccaacggtta tttttacgga attattttgg ttgaattcaa
      721 cgcctgtgtt atcttctctc ttggactttt ctactggggt gtaatcttca tggattattt
      781 tataattccc tcttatttta ggcagctgct ctatatcgct ttttaaaatt cttacaccta
      841 ctcccgagcc tatcattaaa agataaaata agtccctgaa tgcatggaaa tcatttatta
      901 tttcaaatgc acagttgaaa tttgccatag gatagttata tgaaaccgga gttccgccaa
      961 cccaaaatgt tctgcctgac aaaaactgtc ttaaattaaa tatgttatcg tacagttgct
     1021 ctgcttcttc tcttttcgta ggtactaaac tgcagttgta ttcaacggct cttcttaccg
     1081 tttcccacca atactccctt cttctttctt caggaagcca tcttgaataa gttctgtaat
     1141 aaacaaagtt accaagctga tccataggtg agcctctatg cttatacttg ctgataaatt
     1201 cttcgctgag aagtttggaa tctcttctct ttctggagtc cctcgactta tccctctcat
     1261 atctgtataa aatgtatttc tttgcgacat cctttctgac actgtccatg aggtagtttt
     1321 caaccatatc ctgtaccgtt tccacgggta tgggatagtt tttacttgaa agttcctctt
     1381 ctattttcag gcttatttcc ttagccaatt cctcatcaac gccatccttt gtttctttca
     1441 tagccttcat aatagcattg ctgattttat cggcattaaa atcgacaaag tttccggtcc
     1501 tttttaatat tctaatcata tcccttttct ccctgttatt ttatgtggta ctacatattg
     1561 tagcagtaac actaaaatat gtcaatcaaa tactatatat agatattttt tgcacaaatg
     1621 gacaatatca ttgaaatatc attgttttaa aaataatata aaaatttgca aaaaatttaa
     1681 aaaaattcct tta
//
LOCUS       DAOSOW010000400         1672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_72714_length_1672_cov_3.222016, whole genome shotgun sequence.
ACCESSION   DAOSOW010000400 DAOSOW010000000
VERSION     DAOSOW010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72714_length_1672_cov_3.222016"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="PLD67_08855"
     CDS             complement(<1..1076)
                     /locus_tag="PLD67_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HOT22673.1"
                     /translation="MSIEIPGLTQVVIPAAITVHLGAPDEPGENVTVPFLDYIKNVAS
                     SELYPTWPEAALRANIYAITSTALNRIFTEWYRAQGYNFDITNDTRFDQAFVHNRGIF
                     DTVSVLADELFDSYIIRRGQIQPLYAQFCDGRVSWCPGLLQWGTVELAEQGYTPYEML
                     QYYYGEDIDIKTDTPIAEAFETYPGISLQLGDNNPYVLLMQIRLNTIATNYPAIQRIP
                     NPTGTFDEATQEAVRQFQDIFNFPVTGIIDKSTWYGIRRIYAAVTKLAELTSRGIMLS
                     EIPEFTPTPGPEEVIPRVQIVQYFLNVLSAYYNSIPSVDISGVLNTQTRTALMEFQKT
                     FNLPITGLVDEQTWNTMFNAVSGI"
     gene            complement(1087..1554)
                     /locus_tag="PLD67_08860"
     CDS             complement(1087..1554)
                     /locus_tag="PLD67_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOT22674.1"
                     /translation="MKYNPFDGRIYKAQVQPGSIPFELEVGYLSVSVFTASGALPVEG
                     ALVTVYIRDESGAEHEIVHHITDANGRIPLITLPVMYDRLDPFESGRYYFTTYNLRVQ
                     AINYYTVNVIDLRIFPNTTTRFQINMIPVAIGPVEGPDTTIVIPPSPIDISND"
BASE COUNT          496 a          323 c          323 g          530 t
ORIGIN      
        1 aaaattccgg atacggcatt gaacattgta ttccaagtct gctcatctac aagacctgtt
       61 attggcaagt taaatgtttt ttggaattcc attagggcag tcctcgtttg ggtattcaat
      121 acaccgctga tatccacaga aggaatagag ttgtagtatg cggacagaac attgagaaaa
      181 tattgcacaa tttgaactct cggaattacc tcttcaggac cgggagtagg agtgaattcg
      241 ggaatttcgc tcaacataat tcctcttgaa gtcaattccg ctaattttgt aactgcagca
      301 tatattcttc ttataccgta ccatgttgat ttgtcaataa ttcccgtcac cgggaaatta
      361 aatatatcct ggaactgtct tacagcttct tgcgttgctt catcaaaggt tcctgtagga
      421 ttaggtattc tttgtatagc aggatagttt gtggctattg tgtttaaacg tatctgcatc
      481 aataatacat atggattatt gtctcctaat tgaagagaaa ttccgggata agtttcaaag
      541 gcttcagcta tcggagtatc tgtttttata tcaatatctt caccatagta atattgaagc
      601 atttcatatg gggtgtagcc ttgctctgcc aattcaaccg taccccattg gagaagcccc
      661 ggacaccagg agacgcgacc gtcacaaaat tgagcataaa gaggctgaat ttgtccccgt
      721 cttattatgt aactgtcaaa aagctcatct gcaagcaccg atacagtgtc aaaaatacct
      781 ctgttatgaa caaatgcctg gtcaaatctt gtatcatttg ttatatcgaa attataacct
      841 tgagctcggt accattcagt gaatatacgg tttaatgcgg tagaagtaat ggcatatata
      901 tttgctctaa gtgcagcttc cggccaagta ggatatagtt cgcttgaggc aacatttttt
      961 atataatcaa gaaacggaac cgtaacattt tcacccggtt catccggtgc acctaaatga
     1021 actgtaattg ctgcaggtat aaccacttgt gtaagtccgg gaatttctat actcataata
     1081 tcacctctaa tcatttgata tgtcaatcgg actgggagga ataacaattg ttgtatcagg
     1141 accttctacc ggtccgattg caacaggaat catatttatt tgaaaacggg tagtggtatt
     1201 cggaaaaatt cttaaatcaa ttacatttac cgtataatag tttattgcct gaactcttaa
     1261 attataagta gtaaaataat atctgccgct ttcgaaaggg tcaaggcggt catacattac
     1321 gggtaatgtg ataagcggaa ttcgaccgtt agcatcggtt atatgatgca ctatttcatg
     1381 ctccgcacca ctttcatcac ggatataaac cgttactaag gcaccctcta ccggcaatgc
     1441 acctgatgcg gtaaaaacac ttaccgataa gtagcccact tccagctcaa aaggtatgga
     1501 gcccggttga acttgtgctt tgtaaattcg accatcgaat ggattatatt tcaaaatatt
     1561 tacacctctc tatctacata acgaattata ttattatatt gatcataaaa tattttgttt
     1621 atacagtttt ggagcggcta gattgtagcg tcgggtaatt ggtttatgat tt
//
LOCUS       DAOSOW010000401         1666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_73073_length_1666_cov_3.689634, whole genome shotgun sequence.
ACCESSION   DAOSOW010000401 DAOSOW010000000
VERSION     DAOSOW010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73073_length_1666_cov_3.689634"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="PLD67_08865"
     CDS             <1..526
                     /locus_tag="PLD67_08865"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT22675.1"
                     /translation="QLLSDVNPFTDVEGNRWSNRAISTLYNADIISGYPDGTFKPSDP
                     ITRAEFATIAAKFDDLDLGNSSKFSDIFGHWAEKYITSAENKGWINGYPDMTFKPEQD
                     ITRAEAMTLINNVLERAVPAENIHSDAMFWPDIDEDDWYFEAIMEATNSHDYVIEEDG
                     DELWTGMKPNKVWP"
     gene            1092..>1666
                     /locus_tag="PLD67_08870"
     CDS             1092..>1666
                     /locus_tag="PLD67_08870"
                     /inference="COORDINATES: protein motif:HMM:NF015384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HOT22676.1"
                     /translation="MVYYVEYIFVENFIIDFILLFITGKLVKRAIIFKRLIAASLIGS
                     LYVIVTLYAGKEFMNNFIVKFSISVLMIMVAYDSKGILTNIRVIICFYITSLIMVGII
                     SALYILTYDKLTVNAIIMSIFTGYAALHFFFKEIKDRIVKHNYKRKVNVSICGKSKTF
                     RGYIDTGNELTDPITGKPVMVVNIDCIKEILG"
BASE COUNT          558 a          255 c          340 g          513 t
ORIGIN      
        1 ccaactgcta agcgatgtga atccgttcac tgatgttgaa ggtaacagat ggtcaaatcg
       61 tgcaatttca acgctgtaca atgcagacat aataagcggt tacccggacg gaacattcaa
      121 accgtcagac ccgattacga gagcagaatt tgcaactatt gcagctaaat tcgatgattt
      181 ggatttaggc aattcgagta aattctctga tatattcgga cattgggcag aaaagtatat
      241 tacttctgct gaaaacaagg gatggattaa tggttatccg gacatgacat ttaaacctga
      301 gcaggatatc acgagagctg aagcaatgac attgattaac aatgttcttg aaagagctgt
      361 tcctgcagaa aacatacatt ccgatgcaat gttctggcct gatattgatg aagatgactg
      421 gtattttgag gctataatgg aagcaaccaa cagtcacgat tatgttatcg aggaagacgg
      481 cgatgagctt tggaccggaa tgaaaccgaa caaggtatgg ccgtaaagga atatccaaaa
      541 aaagtaataa catttaaata atagcggggc taaatgcccc gcttgtttat gacaaaggat
      601 acaaaatcta atgttttgcc taagctaggt atgacaactt tctctttttg tcattctgag
      661 agcttcagct cgaagaatct caaggcatct ttcacttgcg ctcggtatga cagctctctc
      721 tttctgtcat tctgagagct ttagctcgaa gaatctcaaa ggcccctacg ctatggatga
      781 cataaagtgg ttgtgtacaa cataaactta ttaagtatat tgtagaaggg ttcaaaaaaa
      841 gcagaaaaat gtaaagcata atttttgcgg cgaactatac gaaatacgct atagtattca
      901 taaaaagcgt atttttttat ttttgattac atgttatgac aaaatatttc gataaggtca
      961 tttataattg ttaatagatt gcttggctat agaaacaagg gacgatgggt gagatgcttc
     1021 aggaaaagac tcaggatgac aaattaatca ttctgaaccg cagatgaaga atctcgaggg
     1081 agttggaagg cttggtttat tatgttgaat atatttttgt cgaaaatttt atcatagatt
     1141 ttattttgct ttttattacc ggaaagcttg taaaaagggc aataatattc aaaagactta
     1201 ttgctgcatc tttaatcggc tcattgtatg ttattgttac tttatatgcg ggaaaggaat
     1261 ttatgaacaa tttcatcgta aaattcagta tttccgtatt aatgataatg gtagcatatg
     1321 attccaaagg gatattgact aatatcagag taataatttg tttttacatt acctcattga
     1381 taatggtggg aattatttcc gccttataca ttctaacata tgataaatta accgtgaatg
     1441 caattatcat gtccatattt acagggtatg ctgcattgca tttctttttt aaagaaatta
     1501 aagacagaat tgttaagcat aattacaaaa gaaaggtgaa tgtcagcata tgcggtaaat
     1561 ccaagacatt cagagggtac attgatacgg gcaatgaatt gacagatccg attacaggaa
     1621 aacctgttat ggttgtaaat attgattgca taaaagaaat tttggg
//
LOCUS       DAOSOW010000402         1661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_73395_length_1661_cov_2.921544, whole genome shotgun sequence.
ACCESSION   DAOSOW010000402 DAOSOW010000000
VERSION     DAOSOW010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73395_length_1661_cov_2.921544"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1661
                     /locus_tag="PLD67_08875"
     CDS             <1..>1661
                     /locus_tag="PLD67_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22677.1"
                     /translation="EGPAVEGYRAISLRQIEEDTTGQTIDTYYEKVNGEVVEVSYEGI
                     NVKYLIDNYVKAKSNAGNIIFKDKSRKTIITADISEAGNYMVAYGINEVPLVYLDTDE
                     GYIKEKHNDNGCFKLVYEQDKNKAEEFSNVAYIYIEEKDAKNIFEHSYAPYNAEKYTD
                     YELIIHGSALGKEVRYKVKDIEAMTDLHETHEYSLSNSEYFWYYNTYKGVPLWDLLLK
                     AGLDPDIDENTSVRFIAADNYNFAPMTIKEIKDRSLYGYYEKSAMDKGDGTFPGEYVD
                     PLYTNLPVMVAYGFNKYPYVIRPTDEGYNPGLGNDGGPLRIIFGKTNYNDTNGSNQVQ
                     FAKEIIIGEGESLATDTQGIGEGVSTEIEIDESWNHNQGVYKEYLDMPVLRVTGSQVK
                     QPMTFTLRQIEAMTEYAVRDTYAGDGIREMQGIVLWDLINNVVGLKEDVEVPSIRVFS
                     GQNYNQILRSNEQVVNGVLNSQGQTKKIILAYAADGYPLVPDESSIGYVNNNAYGPLR
                     LIIEESKSMWVKWTDCIVVGTGDYEAPEMKDVKIDTFSDIGSHWA"
BASE COUNT          622 a          256 c          363 g          420 t
ORIGIN      
        1 ttgaaggacc tgcagttgaa ggttacagag ccatatcatt aaggcaaata gaagaagata
       61 caactggaca gacaatagat acatattatg aaaaagtaaa cggtgaggta gttgaagttt
      121 cctatgaagg aataaatgtc aaatatctaa tcgataatta cgtaaaggca aagtctaatg
      181 ccggaaatat tatatttaaa gataaatcaa gaaagacaat tataaccgcc gacatatcgg
      241 aggcaggcaa ctacatggtt gcatacggta taaatgaagt tcccctggtt tacttggata
      301 ctgatgaagg ctatatcaag gaaaaacata acgataacgg ttgttttaaa ttagtttatg
      361 aacaagacaa gaataaagcc gaggaattct ccaatgtagc atatatttac atagaagaaa
      421 aggatgccaa aaatattttt gagcacagct atgctcctta caatgctgaa aaatatacag
      481 attatgaatt gataattcat ggttctgctt tgggaaaaga agtaagatac aaggtaaaag
      541 atatagaagc aatgacagac cttcatgaaa cacatgaata cagtttgtca aacagtgaat
      601 atttctggta ttataacaca tacaagggtg ttcctttatg ggatttactc ttaaaggcgg
      661 gtttagaccc tgacattgac gaaaatacaa gcgtacgatt tattgctgca gataattata
      721 attttgcacc aatgactatc aaagagatta aggacaggtc tctgtacgga tattatgaaa
      781 aaagtgcaat ggataaaggt gacgggacct tcccgggaga atatgtagat cctctttata
      841 cgaatttacc tgttatggtt gcatatggat ttaacaaata tccctatgta ataaggccta
      901 ccgatgaagg gtacaaccca ggtctcggca atgacggggg acctcttcga ataatattcg
      961 ggaaaacaaa ttataacgat acgaacggct ccaatcaggt acaatttgca aaggaaataa
     1021 ttatcggcga aggtgaatct cttgcaacag atacccaagg aattggagaa ggagtcagca
     1081 cggaaataga aatcgatgaa tcatggaatc acaatcaagg tgtatataag gaatatttgg
     1141 atatgccagt actgcgagta acaggctcac aagttaagca acctatgaca tttaccttaa
     1201 ggcaaattga ggccatgact gaatatgcgg ttagagatac ctatgcaggc gatggcataa
     1261 gagaaatgca aggtattgtt ttatgggatt taataaataa tgtggtaggg ctcaaagaag
     1321 atgtagaagt tccaagtata agagtattca gcggacaaaa ttacaaccaa atattaagaa
     1381 gcaacgagca ggtagtaaac ggagtcttaa attctcaagg acagactaag aaaataatcc
     1441 tagcttatgc ggcagacggg tatcccttag tacccgacga gtcaagtatc ggatatgtaa
     1501 acaacaatgc atacggacca ttaagactca taattgaaga aagcaaatca atgtgggtta
     1561 aatggactga ctgcatagta gtaggtacag gggattatga agcacccgaa atgaaggatg
     1621 taaaaattga tactttcagt gatattggaa gccactgggc a
//
LOCUS       DAOSOW010000403         1660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_73450_length_1660_cov_3.849221, whole genome shotgun sequence.
ACCESSION   DAOSOW010000403 DAOSOW010000000
VERSION     DAOSOW010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73450_length_1660_cov_3.849221"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PLD67_08880"
     CDS             <1..629
                     /locus_tag="PLD67_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOT22678.1"
                     /translation="LQMGDGKYIVRVFENISGNKYATKQTVTINVKLKEDKVPFLVPN
                     QLVNFTNTSETVKKAAELTEGITDEFGKIDAIYDYIITNIKYDTEKASTVKSGYLPTV
                     DEILKTSKGICFDYASLMAAMLRSQGIPAKLVTGYSSNLNVFHAWNEVYTEETGWIKL
                     NEMYFDGFQWKLMDSTIASSGKQSNSPKVMQYTNKLIDSKFYTKQFEY"
     gene            complement(868..>1660)
                     /locus_tag="PLD67_08885"
     CDS             complement(868..>1660)
                     /locus_tag="PLD67_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOT22679.1"
                     /translation="KQRNSYAAVIRVVFFELPDPKKLGIPDVVMDLYKKTKGLVLITG
                     PAGSGKSTTLACIIDKINQERNCHIMTFEDPIEFIHKHKKSIVSQREIYTDTNSYISS
                     LRSALRQAPDVMLIGEMRDLETIEIALTAAETGHLVLSTLHTTGAANTIDRIIDVFPS
                     NQQQQVRVQLSMQLQAVISQHLIPSVPSGRVAAFEVMLTNNAVSNLIRESKVYQLNSI
                     IHSSEYMGMKTMDASILELYKKGLITKENALIYATEPDSLLKQLK"
BASE COUNT          536 a          306 c          316 g          502 t
ORIGIN      
        1 cccttcaaat gggagatgga aaatacatag tcagagtatt tgaaaatata agcggtaata
       61 agtatgcaac caaacaaacg gtaaccataa acgttaaatt aaaagaagac aaagtgccgt
      121 ttttagttcc aaatcaattg gttaatttta cgaatacatc tgaaactgta aagaaagcag
      181 ccgaattaac cgaaggaata acagatgaat ttggaaaaat agatgcaatt tatgattata
      241 taataaccaa tataaaatat gacactgaaa aggccagtac ggtaaaaagc ggatatctgc
      301 ctaccgtaga tgaaatactt aaaaccagca aaggaatatg ctttgattat gcttcgttaa
      361 tggccgcaat gcttcgctca caaggtatac cggctaaact ggtaaccggt tattcttcaa
      421 acttaaatgt atttcatgca tggaatgagg tgtatacgga agaaacaggt tggataaaat
      481 taaacgaaat gtattttgac ggctttcaat ggaagcttat ggacagtacc atcgcatcat
      541 cgggcaagca gtcaaacagt cctaaagtta tgcaatatac aaataagtta attgacagca
      601 agttttatac gaaacaattt gaatattaat gattatcccg cattacaagg ggggcgggtt
      661 tttccttcct aatttcgaat taatctcctc atcacactaa aaaatgacca cacagggtca
      721 ttttttagcg tgttattctc tttgctcgga attattacat gttaactaca cttgttatcc
      781 tgagcgcagc gaaggatctc atagattctt cgggcaagca ctcagaatga cactcaaggg
      841 ttatacatag taatgacgac cgaatattta cttgagttgt ttaagtaatg aatccggctc
      901 ggttgcatat atcaatgcat tttcttttgt aatcaaacct ttcttatata attccaaaat
      961 acttgcatcc attgttttca tacccatata ttcagaagaa tgaattatgc tgttaagctg
     1021 atagacttta gattccctta ttaaattgga aacggcattg tttgtaagca taacctcaaa
     1081 agctgcaacc cttcctgacg gcactgaagg aatgagatgc tgtgaaataa ccgcctgcag
     1141 ctgcattgaa agctggactc taacctgctg ctgttgattc gaggggaaaa catcaattat
     1201 tctgtcgatg gtatttgcag cacccgtggt atgcagtgtg gataaaacca aatgtccggt
     1261 ttccgcagcc gttaaagcta tttcgatagt ttccaaatcc ctcatttctc ctatcagcat
     1321 tacatcagga gcttgtctta aggcagaccg aagagaagaa atataactgt ttgtatcagt
     1381 gtaaatttct ctttggctta caattgattt tttatgcttg tgaataaatt ctatggggtc
     1441 ttcaaaggtc attatatgac agtttctctc ctggttaatt ttgtcgatta tacatgccag
     1501 ggttgtggat tttccgctgc cggcaggtcc tgtaataaga acaagcccct tagttttttt
     1561 gtataaatcc atcacaacat ccggtatgcc taatttctta gggtcgggaa gctcaaaaaa
     1621 cactactctg attacggcag cataagaatt gcgctgctta
//
LOCUS       DAOSOW010000404         1634 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_75105_length_1634_cov_3.029132, whole genome shotgun sequence.
ACCESSION   DAOSOW010000404 DAOSOW010000000
VERSION     DAOSOW010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75105_length_1634_cov_3.029132"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1419
                     /gene="lepA"
                     /locus_tag="PLD67_08890"
     CDS             <1..1419
                     /gene="lepA"
                     /locus_tag="PLD67_08890"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013788284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOT22680.1"
                     /translation="VPVINKIDLPSARPDEIKQEIEDVIGLDCSDAPLISAKDNINIE
                     QVLEAIVTKIPPPTGDEDAPLKALVFDSYYDSYRGVVAYIRIKEGQVKKGDKIKMMST
                     GKTFDVTEVGIISPTQKPVPCLYSGDVGYLCASMKEVKSCQVGDTITNNANPAKEPLP
                     GYKKVTSMVFCGIYPAEGEEYNSVRDALEKLQVNDASLIFEPETSVALGFGFRCGFLG
                     LLHMEIIQERLEREFDLNIIATTPSVIYKVYKTDGEMMVLQNPTNMPPVQEIEYMEEP
                     IVAASIMSPTEYVGAIMELCQNRRGTFVNMEYMEAKRVILHYKLPLNEVIYDFFDSLK
                     SKTRGYASLDYELDGYVRSELVKMDILINSELVDAFSIIVHKDKAYERGRAIVERLRD
                     VIPRHLFAVPLQAAIGNKIIARETVKALRKDVLAKCYGGDITRKKKLLEKQKEGKKRM
                     RQIGMVEVPQEAFLAVLKFDED"
BASE COUNT          571 a          253 c          351 g          459 t
ORIGIN      
        1 gttcctgtta taaataaaat tgatttaccc agcgcaagac ccgatgaaat aaaacaagaa
       61 attgaagatg taataggtct cgattgctct gatgcacctc ttatttcagc gaaggacaat
      121 ataaatatcg aacaagtttt agaagccata gtaacaaaga taccgcctcc gacaggcgac
      181 gaagatgcac ctttaaaagc tttggtattt gattcctatt atgacagcta ccgaggtgtt
      241 gtggcatata taagaatcaa agaaggtcag gttaaaaagg gagacaaaat aaaaatgatg
      301 tcaacaggta aaacctttga tgtcacagag gttggaataa tttcacctac acagaagccg
      361 gtaccttgtc tttattcggg agatgtgggt tatttgtgtg ccagcatgaa ggaagtaaaa
      421 agctgtcagg ttggagatac aataactaat aatgcgaatc ctgcaaaaga gcctttgccg
      481 ggatataaaa aggttacgtc catggttttc tgcggtattt atccggcaga gggagaggaa
      541 tacaacagtg tgagagatgc attggaaaaa cttcaggtaa atgatgcttc cttaattttt
      601 gagccggaaa catcagttgc cttaggattt ggattcagat gcggattttt gggtcttttg
      661 catatggaaa taattcagga aaggcttgaa agggaatttg atttaaatat tatagcaaca
      721 actccaagcg taatatacaa agtatataag actgacggag aaatgatggt tttgcaaaat
      781 cctacaaata tgcctcccgt acaggaaatc gaatatatgg aagaaccgat agttgctgca
      841 tccataatga gtcctaccga gtatgtggga gcaattatgg agctatgcca aaaccgcaga
      901 ggtacatttg taaatatgga atatatggaa gcgaaaagag ttattctaca ttataaatta
      961 cctttgaatg aagtaatcta tgatttcttt gattccttaa aatcaaaaac aagaggatat
     1021 gcatcattgg attacgagct tgacggttat gtacgctccg aattggttaa aatggatatt
     1081 ctaataaatt ccgaattggt agatgccttt tcaataattg ttcataaaga taaagcttat
     1141 gaaagaggaa gagcaatagt tgaaagactt agagatgtaa tcccgaggca tttgtttgca
     1201 gtgcctcttc aagctgcaat aggaaataaa ataattgcaa gagagaccgt aaaagcactc
     1261 agaaaagatg ttcttgcaaa gtgttacggc ggagatataa ccagaaagaa aaagcttttg
     1321 gaaaagcaaa aagaaggcaa gaaacgaatg agacaaatag gaatggtgga ggttccacag
     1381 gaagcattcc ttgcagtttt gaaatttgat gaagattaag tatgccctaa ccccatttat
     1441 cagagtattt tttctgataa atggttggta ggtcaaccgc acagaatgcc aaatttgtgc
     1501 gacgaaagga gcaaacaaat ttgatgcatt cccctgcgta tgcccttatc ccatttgatg
     1561 cattcacctg tatgtccctt taaataagtt taaataattt ttaagtaatt ttttaaactt
     1621 aagtgtttaa ttat
//
LOCUS       DAOSOW010000405         1623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_75805_length_1623_cov_3.102041, whole genome shotgun sequence.
ACCESSION   DAOSOW010000405 DAOSOW010000000
VERSION     DAOSOW010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75805_length_1623_cov_3.102041"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="PLD67_08895"
     CDS             <1..571
                     /locus_tag="PLD67_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOT22681.1"
                     /translation="LKDSKKILQQAKDELTNEGIKFKKDIEIGIMIEIPSAAIISDLL
                     AREVDFFSIGTNDLIQYTMAVDRMNSKLSYLYSQYHPALLRLIKGIIDNAHEAGIWVG
                     MCGEAAGDPKLIPVFVGMGLDEFSMNSPSILRARYIVRNLNKADMEIIAKSTLNMENA
                     LEVEEYLSCVFADENESCLRTCKNRRRQE"
     gene            568..1149
                     /locus_tag="PLD67_08900"
     CDS             568..1149
                     /locus_tag="PLD67_08900"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOT22682.1"
                     /translation="MSKIVSIVFHSICGNNFLISKEFYKEFKAHNADVQLLRVKDINF
                     DELAKQFTIGTQYKKEILNIEEAKVEKLLDSDIIILGSPTYFGNVSGAMKAFMDEFYP
                     YWQSEKFYGKKMLAFTTCANGEGGGDLCLNAMTVFAQNMGMITVPVPKNLVSGQNFPA
                     NGLIHYVGDYSNLRPSGKIIPAIKEMTQKLMEM"
     gene            1282..>1623
                     /locus_tag="PLD67_08905"
     CDS             1282..>1623
                     /locus_tag="PLD67_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil-5-)-methyltransferase RumA"
                     /protein_id="HOT22683.1"
                     /translation="MKKNDIIEVAINDLEFPNIGTTNFENKKIRIKNTLPGQKVRAKV
                     VRSRKESADARLIEVLENAYYEKEPVCEHFNLCGGCTYQTVPYKNQLEIKEKQVKNIL
                     DEILTEKYEFLP"
BASE COUNT          622 a          248 c          327 g          426 t
ORIGIN      
        1 attaaaagac tcaaagaaaa tactgcagca agcaaaagat gaattaacaa atgaaggcat
       61 aaaattcaaa aaagatatag aaataggtat catgattgaa ataccttctg ctgcaataat
      121 atctgacctt ttagccaggg aggttgactt tttcagtatt ggaaccaatg atttgattca
      181 atataccatg gctgttgaca ggatgaattc caaattatca tatttgtaca gccaatacca
      241 cccggcactt ttaagactaa taaagggtat aattgacaat gcacatgaag ccggcatctg
      301 ggtcggcatg tgtggtgaag cagccggaga ccccaagctt ataccggttt tcgtgggaat
      361 gggactagac gaattcagca tgaattctcc ttcgattctc agggcaagat atatagtgag
      421 aaatttaaat aaagcagata tggagataat agcaaaaagt accctcaata tggaaaacgc
      481 acttgaggta gaggagtatc tgagctgcgt gtttgcggat gaaaacgaaa gctgcttaag
      541 aacttgcaaa aataggagaa gacaagaatg agcaaaatag tatcaatcgt atttcacagt
      601 atttgcggaa ataactttct catatcgaaa gaattttata aagaattcaa agctcacaat
      661 gcagatgtgc aattgttaag agtaaaggat ataaactttg atgaacttgc aaagcaattt
      721 acaataggca ctcaatataa aaaagaaata ttgaatatag aagaagcaaa agtagaaaag
      781 cttcttgaca gcgatataat aatacttggt tcacccacat attttgggaa tgtttcaggt
      841 gccatgaaag cattcatgga tgaattttat ccctattggc agagtgagaa gttctatggc
      901 aaaaagatgt tagcctttac aacctgtgcc aacggagaag gcggagggga cttatgcttg
      961 aatgcaatga ctgtctttgc tcagaatatg ggaatgatta cagtgccggt tcccaaaaac
     1021 ttagtctccg gtcagaattt tccggcaaac ggattaattc attatgtagg agattattca
     1081 aatctaagac caagcggtaa aataattcct gcaataaaag aaatgacaca aaagcttatg
     1141 gaaatgtaaa ttattataaa tggggacatt ccttttatct tgacagaagg tgtgtcccca
     1201 tgtcattatg agaccattag ctcgaagaat ctcatacatt gtcattctga acgcagtgaa
     1261 gaatctaatc aaggagtaca aatgaaaaaa aacgatataa ttgaagttgc aattaatgat
     1321 ttagagtttc ctaatatagg aacaacaaat tttgaaaata aaaaaataag aataaaaaac
     1381 actttacccg gacaaaaggt aagggctaag gttgtacgtt cacgaaaaga aagtgcagat
     1441 gcaagactaa tagaagtatt agaaaatgca tattatgaga aagagccggt ctgtgagcat
     1501 tttaacttat gcgggggctg tacctatcaa actgtaccat acaagaacca gcttgaaata
     1561 aaagaaaaac aggttaaaaa tatattagat gaaatattga cagaaaaata tgaatttcta
     1621 cca
//
LOCUS       DAOSOW010000406         1620 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_76021_length_1620_cov_2.070927, whole genome shotgun sequence.
ACCESSION   DAOSOW010000406 DAOSOW010000000
VERSION     DAOSOW010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76021_length_1620_cov_2.070927"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="PLD67_08910"
     CDS             complement(<1..338)
                     /locus_tag="PLD67_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOT22684.1"
                     /translation="MNFDVKRVFVEKRAGFNTEALNLLNNLKENLNIEKLNNLRILNR
                     YTVSNIDEDDFKKSVNTVFSEPNQDIVYFEDVELEGHVFAVEYLPGQYDQRADFAAQC
                     IQIVTGKEKPT"
     gene            complement(595..>1620)
                     /gene="buk"
                     /locus_tag="PLD67_08915"
     CDS             complement(595..>1620)
                     /gene="buk"
                     /locus_tag="PLD67_08915"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOT22685.1"
                     /translation="TKIALYEDEKEVFTKNLSHPDELINKFDDVKDQLDMRLEFVKSY
                     LKEKQFDIKELSCVVARGGMLLPVKSGAYVINDIMLDRVENRPVMEHASNLAAPIAYG
                     IAKEAGVTSYIYDSVMTDEFPDISRISGMPDIPRKSASHVLNTRAMAIKYAKSIGKSY
                     EELNTIVAHLGGGISINIHHKGQIIDLVSDDEGPFSPERAGRVPCRELINLCYSGKFD
                     KKTMQRKLRGSGGLKAYLNTIDAREVESMIKEGNEYAKLIYEAMAYQIAKGIGELATV
                     VEGKVDVIILTGGIAHSEMLTSWIKNRVSFISHVEIMPGENEMEALAFGALRVLRGEE
                     KAREYIE"
BASE COUNT          470 a          333 c          223 g          594 t
ORIGIN      
        1 gtaggttttt cttttccggt gactatttgt atgcattgag ccgcaaaatc ggctctttgg
       61 tcatattgtc ctggcaggta ttcaacggca aaaacatgtc cttcaagctc aacatcttca
      121 aaatacacaa tatcctgatt aggctctgaa aaaacagtat ttacactttt tttaaaatca
      181 tcttcatcaa tatttgatac cgtatatctg tttaatattc taaggttatt taatttttca
      241 atatttaaat tttcctttaa gttatttaat aaatttaatg cttcggtgtt aaaccctgct
      301 cttttttcaa caaacaccct cttaacgtca aagttcataa ataaaccttc ctttttatca
      361 ttttacaaaa atataataac aaatataaaa atccttgtca acggtttata tagtaatttc
      421 aacaaaaaat atattttatg ttcgtactat accgaattat taattaatca tgtttcctta
      481 tattcgtatt attctgaacc ttgcaaaata ttccaattaa tagagggaat gggggttaac
      541 tataaaaaaa acagtaacct tacgattact gtttttatta ttacccttcc ttttttattc
      601 aatatactct ctagcctttt cttcacctct gagaactctt agagcgccga aagccaaagc
      661 ttccatttca ttttcaccgg gcattatttc tacatgagat ataaaagata ctctgttttt
      721 tatccaggaa gtaagcatct cagaatgtgc aatacctccg gtaagaatta taacatcaac
      781 cttgccttct accactgtag ccagttctcc gattcctttt gctatttgat atgccattgc
      841 ttcataaatc aattttgcat attcattgcc ctctttaatc atagactcga cttctcttgc
      901 atcaatggtg tttaaatatg cttttaagcc tccgcttcct cttagctttc gctgcattgt
      961 ttttttatca aatttacctg aataacaaag atttatcagc tctctgcacg gtactcttcc
     1021 tgctctttca ggcgaaaagg gtccttcatc atcagaaacc aagtctatta tctgaccctt
     1081 gtgatgaata ttgattgaaa ttcctcctcc taaatgcgca acgatagtgt tcagctcttc
     1141 gtaacttttg ccgatacttt tagcgtattt tatagccatt gcccttgtat ttaaaacatg
     1201 gcttgcgctt tttctgggta tatcaggcat gccggatata cgagaaatgt caggaaattc
     1261 atctgtcatc acggagtcat atatatatga agttacgccg gcttcctttg caataccgta
     1321 agcaatcgga gctgcaagat ttgatgcatg ctccattacg ggacgatttt ccaccctgtc
     1381 gagcataata tcattaataa catatgctcc cgattttacc ggaagaagca ttccccctct
     1441 tgcaacaaca caagataatt ccttaatgtc aaattgcttt tcttttaaat aggatttgac
     1501 aaattctaat ctcatgtcaa gctggtcctt gacatcatcg aatttattga ttaattcatc
     1561 aggatgactg agattctttg taaatacttc tttttcatct tcatataatg ctattttggt
//
LOCUS       DAOSOW010000407         1597 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_77515_length_1597_cov_1.880026, whole genome shotgun sequence.
ACCESSION   DAOSOW010000407 DAOSOW010000000
VERSION     DAOSOW010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77515_length_1597_cov_1.880026"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PLD67_08920"
     CDS             complement(<1..705)
                     /locus_tag="PLD67_08920"
                     /inference="COORDINATES: protein motif:HMM:NF013942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOT22686.1"
                     /translation="MKKVGIIIGGKSVEHEVSIITGLQVFENIDKDIYEPKIIYIQKD
                     GKWLVGEGLHDINNFKTKKFDDAYEVLPGAKNEKLILYPHPELKTGFFGKKYTTYEID
                     IVFPAVHGTNVEDGALQGLLQMNGVPYAFGSVLSSAVGMDKVVMKKVFQSCNLPIVDY
                     IWFFRSSFEESKDKIFEQAEKIGYPLIVKPANLGSSVGINKANNCDELLFAIEVAMSY
                     DRKVIIEKCLENGREIN"
     gene            complement(724..>1597)
                     /gene="murF"
                     /locus_tag="PLD67_08925"
     CDS             complement(724..>1597)
                     /gene="murF"
                     /locus_tag="PLD67_08925"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOT22687.1"
                     /translation="GATKTGDIDEVAKLTNPKIVIITSIGPCHLETFKSIDNIMRTKY
                     ELIENLPDDGIAIFNYDNEYVKRLADKTFKEKLLYGIDNIEDTDVFATNIHVDSLGSS
                     FSLCIKSLGVIECRTKLLGKHNILNILGGAAIASVLGLSLDEIKRGISNLETVEHRLQ
                     LIDNGLGILVIDDAFNSNPDGAKAALDVLNSFTDRRKIIVTPGMVELGDIEETENFKF
                     GEQIASVCDIAILVGKKRTLPIYQGMKKHNFNEDNLYVVNSLNEATEILKSLTKVNDV
                     VLFENDLPDTYDEK"
BASE COUNT          474 a          330 c          215 g          578 t
ORIGIN      
        1 gtttatttct ctaccatttt ccaaacattt ttctataatg acttttctgt cataagacat
       61 tgcaacttca atggcaaata ataattcatc acaattattt gctttgttaa taccaacgct
      121 ggagcctaaa ttagcaggct ttacaatcaa aggatatcct atcttttctg cctgctcaaa
      181 aattttatct ttactttctt caaaactgct tctgaaaaac catatataat ctacaatagg
      241 caggttacag ctttgaaaca ccttcttcat tactacctta tccattccga ctgcagagct
      301 taacacggag ccaaaggcat aaggaacccc gttcatttgt aaaagtccct gcagtgcacc
      361 atcttcaaca tttgtaccgt gaacggcagg aaatactatg tcaatttcat atgtagtata
      421 tttttttccg aaaaaacctg ttttaagctc aggatgagga tagagaatca gcttttcatt
      481 tttagctccc ggcaaaacct catatgcatc atcaaatttt tttgttttaa aattgtttat
      541 gtcatgaaga ccttcaccaa ctaaccattt accgtccttt tgaatataaa ttatcttagg
      601 ttcatatatg tctttgtcta tgttttcaaa aacctgcaag cctgttatta ttgaaacttc
      661 atgttcaacg gattttccgc cgataataat accaactttt ttcatggctt cctccctaaa
      721 attttatttt tcgtcgtatg tgtcgggtaa atcattttca aaaagaacaa catcattaac
      781 tttagtcaat gatttcaata tttccgtagc ttcattcaat gaattaacca catacaaatt
      841 atcttcattg aaattatgct ttttcattcc ttggtatatg ggcaaagtac gttttttccc
      901 aaccagtatt gcaatatcac aaacacttgc aatctgctca ccaaatttaa agttttctgt
      961 ttcttctata tctcctaatt caaccattcc cggtgtcact attatttttc ttctgtccgt
     1021 aaaagaattc agcacatcta atgcagcctt tgctccgtcc ggattagaat taaaagcatc
     1081 atctattacc aatataccta atccgttatc aataagctga agtctgtgct ccacagtttc
     1141 caaatttgaa attcctcttt ttatttcatc taatgacaaa cctaaaacag atgcaatagc
     1201 tgctccacct aatatattaa gtatattgtg cttgcctaat agtttagtcc tgcattcgat
     1261 tactcccaag cttttgatgc ataaggaaaa agatgagcca agactatcca catggatgtt
     1321 ggttgcaaat acgtctgtgt cttctatgtt gtctataccg tagagtaact tttccttaaa
     1381 agttttatcg gcaagtcttt ttacatattc attatcataa ttaaaaatag ctataccgtc
     1441 atccgggagg ttctcaataa gttcatactt tgttctcata atattatcta ttgatttaaa
     1501 tgtttctaaa tggcatggtc cgattgatgt tatgataacg attttaggat ttgtaagctt
     1561 tgcaacctca tcaatgtcac ctgttttggt agccccg
//
LOCUS       DAOSOW010000408         1592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_77835_length_1592_cov_3.195836, whole genome shotgun sequence.
ACCESSION   DAOSOW010000408 DAOSOW010000000
VERSION     DAOSOW010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77835_length_1592_cov_3.195836"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..561)
                     /locus_tag="PLD67_08930"
     CDS             complement(91..561)
                     /locus_tag="PLD67_08930"
                     /inference="COORDINATES: protein motif:HMM:NF022604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HOT22688.1"
                     /translation="MRFFYGERNILRALEEADFWKKQESEHVDVILEIVPDLEEEYIQ
                     KLEEYQEIFNSTHGRIVQLVETAVNCHNILSPELQRDIINIINLAVRQSQVFIDFIGM
                     LLRDSKAVTNNPTAIIVLNHIRRESEYFVGIVTAFLNSSCANNGRYYNDTGFQR"
     gene            complement(641..862)
                     /locus_tag="PLD67_08935"
     CDS             complement(641..862)
                     /locus_tag="PLD67_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22689.1"
                     /translation="MIAIKGRRKCANCGKRYNYSYAKGEEALKKAKFKGKEENLSTVT
                     NVKVLSVHTFEVTATCPECGYEEVFIYND"
     gene            complement(884..>1592)
                     /locus_tag="PLD67_08940"
     CDS             complement(884..>1592)
                     /locus_tag="PLD67_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020156709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOT22690.1"
                     /translation="HWGGFPKANEATSGYLIEHDNYKLLLECGSGVVSSLQKITDIKN
                     IDAVLITHYHYDHCCDIGPLQYAIQIKTQLKEINAPLPIYAPTGEFFNLLKWENYTYG
                     ISFEENSNLTLGPFEISFIKNIHPVEAYSVRIKCGDKVFSFTSDTSYFENLAEFFSNS
                     DLLICECSFYSDMDGLKAGHLNSSQAGMLAEKANVKNLILTHLPHFGDLNNLLTEAKE
                     FYKGEIELASYMMEIDI"
BASE COUNT          453 a          317 c          238 g          584 t
ORIGIN      
        1 acgctaaccc cgcccatttt ttatgccttc ttatatatcc catcccctgt aataagtcct
       61 tctttttaat ccctataatt gctttgtcac ttatctttga aatcccgtat cattataata
      121 tctgccatta ttagcacaag agctgtttaa gaaagcagtt acgattccta cgaagtattc
      181 cgattctctt cttatgtgat taagaacaat tatcgccgta ggattgtttg taacggcttt
      241 gctgtctctt agcagcatac ctataaagtc aatgaaaacc tggctttgtc ttactgctaa
      301 attaattata ttaataatat ccctctgtaa ttcgggagat aatatgttgt gacaatttac
      361 agctgtttcc acgagctgga ctattcttcc gtgtgtagaa ttgaatattt cttgatattc
      421 ttctaatttt tgaatatatt cttcttctaa gtcaggaact atttctaaaa taacatcaac
      481 atgttcactt tcctgcttct tccaaaaatc agcttcttct aaagccctta atatattcct
      541 ttcaccataa aagaatctca tcgtacatcc tccaaataat tatttggtat attatacgta
      601 tgagattctt ttatgttatt attaaggtta aataccttta ttaatcatta tatatgaaca
      661 cttcttcata gccgcattcc gggcaggtag cggttacttc gaaggtatgt acggaaagca
      721 cttttacatt tgtaacagtg cttagatttt cctctttgcc tttaaatttg gctttcttta
      781 aagcttcttc gccttttgcg tatgaatagt tatatctttt gccgcaattt gcgcattttc
      841 gtctgccttt tattgctatc atggtatcac ttccctaaga gtattatata tctatctcca
      901 tcatatatga tgctaattca atttcaccct tataaaattc ctttgcttca gtcaataaat
      961 tatttaaatc gccaaaatga ggcaggtggg taagtataag attctttaca tttgccttct
     1021 ctgcaagcat tcctgcttga gagctgttaa gatgtcctgc ctttaatccg tccatatctg
     1081 aataaaagct gcattcgcaa atcaataaat cagagtttga aaaaaactct gccaagtttt
     1141 caaaatatga agtatccgaa gtaaatgaaa atactttatc gccgcatttt attctaacgc
     1201 tgtatgcttc aacagggtga atatttttaa taaagcttat ttcaaatggt cccagagtta
     1261 aattgctgtt ttcttcaaaa cttatgccgt aagtgtaatt ttcccactta aggagattaa
     1321 agaattctcc cgtcggtgca taaatgggca gtggagcatt aatttcctta agctgcgtct
     1381 tgatttgtat tgcatactgc aagggtccta tatcacagca atggtcataa tgataatgcg
     1441 ttatcaatac agcatcgatg ttctttatat ccgtaatttt ctgcagggag cttacaactc
     1501 cgctgccgca ttctaaaagg agtttataat tatcatgttc aatcaaatat cctgaagttg
     1561 cttcattagc cttcgggaat ccaccccaat ga
//
LOCUS       DAOSOW010000409         1578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_78853_length_1578_cov_2.577807, whole genome shotgun sequence.
ACCESSION   DAOSOW010000409 DAOSOW010000000
VERSION     DAOSOW010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78853_length_1578_cov_2.577807"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>288)
                     /locus_tag="PLD67_08945"
     CDS             complement(<1..>288)
                     /locus_tag="PLD67_08945"
                     /inference="COORDINATES: protein motif:HMM:NF014235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22691.1"
                     /translation="TLNIETELEKVETNSRFIQSINYNDTVVIIILNVVLNELSGKIT
                     LCIPSNIIDEILKGANIYSKNISKKVNIEEQKTAIMNSLKSSYLTITGVLGS"
     assembly_gap    289..298
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<299..822)
                     /locus_tag="PLD67_08950"
     CDS             complement(<299..822)
                     /locus_tag="PLD67_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOT22692.1"
                     /translation="MAEVLSQSQIDELLNGLSTGDVSIQELEENEKRIKEYDFRSPKK
                     ITKETIRLLKGIYENYCRMLTSRLTSMLRLMCDVTVEQVEESIYHEYNNALEDYVLMG
                     LINVKYPDNETSDSQILMEMSRPLSFVIIDRLLGGIGEEYGYIRDYTDIELSLLSNVL
                     NQLVILMGTTWENT"
     gene            complement(850..>1578)
                     /locus_tag="PLD67_08955"
     CDS             complement(850..>1578)
                     /locus_tag="PLD67_08955"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOT22693.1"
                     /translation="TFFIMLYAMSTIDAQKFAAIVASFSKEMNPEAYYQMMEAEAAEQ
                     VVDDTYEETEETEEIEDIDVLYTLLKQYIEENELAGQVEISKSEEYVFIRFSDSVTFR
                     GYSDILEQKGKGVLDVLAEGLAMVDEYIEEVVIAGHTAKVEYDMTDIDRSLSTNRANV
                     VLRYLENKDVIDPAKYLAIGYGLYSPIADNSTAEGRAKNRRVEIYISRKGHPISYTRI
                     IQETINNGKTDNNDLEYRRDIYIE"
BASE COUNT          418 a          320 c          214 g          616 t
ORIGIN      
        1 acttccgagc actccggtta tagttaagta tgatgatttt aatgaattca taattgctgt
       61 tttttgctct tcaatattaa cttttttact tatattcttt gaatagatat ttgcaccttt
      121 cagtatttcg tcaataatgt ttgaaggtat gcataaggtg attttccctg acagctcatt
      181 taatactaca tttaatatta ttattactac ggtatcattg taatttattg actgaataaa
      241 tctggagttg gtttcaactt tttctaactc ggtttctata tttaaagtnn nnnnnnnnaa
      301 agtgttttcc catgttgttc ccattagaat aaccaactga tttaatacat tgctgagcag
      361 tgacagctca atatccgtgt aatctcttat atatccgtat tcttctccga ttcctccaag
      421 aagcctgtct ataataacaa aggacaaggg tctggacatt tccataagaa tttggctgtc
      481 agaagtttca ttatccggat attttacatt gataagaccc attaatacat aatcctctaa
      541 ggcattgttg tattcatggt atatgctttc ttccacctgt tctaccgtta catcgcacat
      601 cagcctgagc attgatgtta atcttgaagt aagcattctg cagtagtttt cgtaaatacc
      661 ctttagcaat cttatggttt cctttgtgat tttcttaggg cttctgaaat catattcttt
      721 aattcttttc tcattttcct ctaattcttg tatacttaca tcacctgtgc tcaaaccgtt
      781 aagcaactca tctatttgac tttgtgatag tacttccgcc atatttaatc ctcattccat
      841 aattttatac tattctatat atatgtctct tcgatattcc aaatcattat tgtcagtctt
      901 gccgttattg atagtttctt gaattattct tgtataactt atggggtgtc cttttcggct
      961 tatgtatatt tcaactcttc tgtttttagc cctgccttca gcagtactgt tgtcagctat
     1021 gggactgtat aatccatatc ctattgccag gtatttagca gggtctataa catccttatt
     1081 ttctaaatat cttagaacaa cattggctct gttcgtagat aatgacctgt caatatccgt
     1141 catatcatat tcaaccttag cagtgtgacc tgctataaca acttcctcta tatattcgtc
     1201 gaccattgct aacccttccg ctaatacatc gagcactcct tttccttttt gctctaatat
     1261 gtcgctatac cctctaaaag ttaccgaatc ggaaaatctt atgaacacat attcttcact
     1321 tttacttatt tctacttgac ctgccaattc attttcttca atatattgct taagaagcgt
     1381 atacaataca tcaatatctt ctatttcttc tgtttcttct gtttcctcat atgtatcatc
     1441 taccacttgc tctgcagctt cagcttccat catctgataa tacgcttcag gattcatttc
     1501 tttagaaaac gaagcaacta ttgcagcaaa tttttgggca tcaattgttg acatagcgta
     1561 aagcattata aaaaaggt
//
LOCUS       DAOSOW010000410         1573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_79156_length_1573_cov_3.517128, whole genome shotgun sequence.
ACCESSION   DAOSOW010000410 DAOSOW010000000
VERSION     DAOSOW010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79156_length_1573_cov_3.517128"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1573)
                     /locus_tag="PLD67_08960"
     CDS             complement(<1..>1573)
                     /locus_tag="PLD67_08960"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOT22694.1"
                     /translation="AKENSSLPVFATMSFQDKSRTFTGNDVKSFVFTAEGLGVDVLGV
                     NCSLGPKQLQPIVDELLKYSSIPIMVQANAGLPKYRNNTAYYDIDPSKYSHEINIMAK
                     KGVAIFGGCCGTTPEYIKSIVNALDGLKPIKIVKKNFTTAASSTKTVFLGDEVKIIGE
                     RINPTGKKRLKEALRENNLNYIVREAIEQEDSGSDILDINVGLPGIDEKEMMKKVIKE
                     VQSVVSLPLQIDSADYEVIEAAARIYNGKPIINSVNGKKESMDKIFPIAKKYGALVVA
                     LTLDEEGIPATSEKRLEIATNIIETAKVYGIPEENILIDPLVLTASAEQKAVMETLKA
                     IPLIKNKYNVKIVLGTSNVSFGLPKRNLLNTTFLSMALGFGLDAPITDSTNKSLMDVV
                     RSFKVLANQDKDSKDYIKTYTNISYENNIPSSETDLKEIIIKGLKDEAKNVSIKMLEQ
                     YNSLDIVNEFMIPALDIVGEKYEKEEIFLPQLIRSAETSKIVFEVIKEKSINEKITDD
                     KKILLATVKGDIHDIG"
BASE COUNT          446 a          307 c          230 g          590 t
ORIGIN      
        1 ccctatatcg tgaatatctc ccttgactgt tgccaaaagt atttttttgt catcagttat
       61 tttttcatta attgattttt ccttaattac ttcaaaaact attttggaag tttctgcact
      121 tcttattaac tggggcagaa atatttcctc tttttcatat ttttcaccta caatatccaa
      181 ggcaggtatc atgaattcat ttactatatc aagcgagtta tattgctcca acatttttat
      241 agaaacgttt ttagcttcat cttttagacc ttttataata atttctttta agtcggtttc
      301 agatgaagga atattgtttt cataggatat atttgtgtat gtcttaatat aatccttgct
      361 gtccttatct tgattggcca gtaccttaaa ggaccttaca acatccatca aagacttatt
      421 tgttgaatcc gtaataggtg catccaaacc aaaacctaaa gccatagata aaaaagttgt
      481 atttaataaa tttctcttag gcagcccaaa agaaacattg cttgttccca atactatttt
      541 tacattatat ttatttttaa ttaaagggat tgctttaagt gtttccatta cggctttctg
      601 ctcggcagaa gctgtaagaa cgagcgggtc tattaatata ttttcttcag gaataccgta
      661 tacttttgct gtttcaataa tatttgttgc tatttccaat cttttttcag aagttgcagg
      721 aataccttcc tcatctagtg ttaaagccac taccaaggcc ccatattttt ttgcaatcgg
      781 aaatatttta tccatacttt ctttcttgcc gtttacagaa ttaattatgg gctttccgtt
      841 atatattctt gcggcagctt caataacttc ataatcagca ctatcaattt gaagaggcaa
      901 tgaaacaaca ctttgcactt ctttgattac ctttttcatc atctcttttt cgtcaatacc
      961 gggaagtccc acattgatat ctaatatatc agagcctgag tcctcttgtt ctatagcttc
     1021 tcttacaata tagttcaggt tattttctct aagggcttct tttaatctct ttttacctgt
     1081 cgggttaatt ctttcgccga taattttaac ttcatcccct aaaaatactg tcttggtcga
     1141 tgaagcagca gtcgtaaagt tcttttttac gatttttatc ggttttaatc catccaatgc
     1201 attaacaata cttttaatat attcaggagt agtgccgcaa catcctccaa atattgcaac
     1261 acctttttta gccattatat ttatttcatg actgtatttt gaagggtcga tatcataata
     1321 agcagtattg ttgcgatatt taggcagccc cgcatttgcc tgtaccatga ttggtattga
     1381 gctgtatttt aacaactcat caacaatcgg ctgaagctgt ttgggcccta aggagcagtt
     1441 aacgcccaaa acatcgacgc ccaaaccttc agccgtaaaa acaaatgatt ttacatcatt
     1501 acccgtaaat gttcgactct tatcttgaaa agacattgtt gcaaaaacag ggagtgaaga
     1561 attttcctta gca
//
LOCUS       DAOSOW010000411         1572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_79251_length_1572_cov_2.493738, whole genome shotgun sequence.
ACCESSION   DAOSOW010000411 DAOSOW010000000
VERSION     DAOSOW010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79251_length_1572_cov_2.493738"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..1566
                     /locus_tag="PLD67_08965"
     CDS             34..1566
                     /locus_tag="PLD67_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT22695.1"
                     /translation="MLKKNDNYAIELKGITKSFGKVIANEDVNLGIKYGEILALLGEN
                     GSGKTTLMNILSGIYYADEGTIFIDGNEVFITNPHEAIKLGIGMIHQHFKLVEVFSAM
                     DNIVLGTKGRYVKNSALKEKILEMSNKFGLEIEPDKKVYNMSVSEKQTIEIMKVLYRG
                     AKILILDEPTSVLTPQETEKLFVILRRMKEQGNAIIIITHKLHEVLAISDRVAILRKG
                     KVVDTVNTRETNEKQLTELMVGRPISLEIKRPETSRKKTVLKVVDLSVVNEDGTIGLN
                     NINFKLKSGEILGVAGVAGSGQRELCETIAGLMHPKTGAVLYNKENIIGKTPDEIINL
                     GISMSFVPEDRLGMGLVGSMDMIDNMLLKSYKKNKGFIIDKKPSRQLAEELVDKLSIT
                     TPNVETPVRMLSGGNVQKVLLGREIESEPNVLITAYPVRGLDINSSYIIYDLLNEQKK
                     KNVAILFIGEDLDVLLELSDRIMVLCHGELMGLVDAKNTTKEEIGLMMAGRKMEGVDS
                     DE"
BASE COUNT          575 a          211 c          364 g          422 t
ORIGIN      
        1 gtttacgctc aggatatgac aaaaaaggtg attatgttga aaaagaatga taattatgca
       61 attgagctca agggtattac taaatccttt ggaaaagtaa ttgcaaacga agatgttaac
      121 cttggaatta aatatggtga aattcttgct ttgcttggag aaaacggctc gggaaaaacc
      181 acattgatga atattttatc cggcatttat tatgctgatg aaggtacaat atttattgat
      241 ggaaatgaag ttttcataac aaatccacac gaagctataa aattaggcat aggaatgatt
      301 caccagcatt ttaaattggt agaagtattc agtgcaatgg ataatattgt tttaggcaca
      361 aagggaagat acgtcaaaaa cagtgcttta aaagaaaaaa ttcttgaaat gagcaacaaa
      421 ttcggtttag aaatagagcc tgataaaaaa gtctacaata tgtccgtgag cgagaagcag
      481 accattgaaa taatgaaagt gctgtacagg ggggcgaaga ttttaatttt agacgagccc
      541 acttcggtac tcacacctca ggaaactgaa aagctctttg tgatacttcg cagaatgaaa
      601 gagcagggga atgcgataat tattattaca cacaagctcc atgaagtttt agcaatcagt
      661 gacagggttg caatacttcg aaaaggaaag gtagtcgata cggtcaatac tcgggagact
      721 aatgagaaac agcttacgga attaatggtg ggaagaccta tcagccttga aatcaagcgt
      781 cccgaaacaa gcagaaagaa gacagtttta aaggtggtag acctgtctgt tgtaaatgag
      841 gacggcacaa taggtttaaa caatattaat tttaagctaa aaagcggaga aatactaggt
      901 gttgcaggtg ttgcaggaag cgggcaaaga gaattgtgcg aaactattgc aggacttatg
      961 catcctaaga caggagccgt attatataac aaggaaaata ttatagggaa aactcccgat
     1021 gaaattataa atttaggcat aagcatgagt tttgtaccgg aggacaggtt aggcatgggg
     1081 ttagtaggct ctatggacat gattgataat atgttgctta aatcatacaa gaaaaacaaa
     1141 ggttttataa ttgacaaaaa accgtccagg cagttagccg aagaattggt tgataaactg
     1201 agtattacaa ctccgaatgt tgaaactccg gtaagaatgc tttcaggagg aaatgtacag
     1261 aaggttcttt tgggaagaga aattgaatct gaaccgaatg ttttgattac tgcatatccc
     1321 gtcagaggac ttgatataaa ttcttcttat ataatttatg atttattaaa tgagcaaaag
     1381 aaaaagaatg ttgcaatatt atttatagga gaagatttgg atgttttgtt ggagctgtcc
     1441 gacaggatta tggttttatg ccatggagaa ttaatgggtc ttgtggatgc aaaaaatact
     1501 acaaaggaag aaatcgggct tatgatggca ggaagaaaaa tggaaggagt tgactcagat
     1561 gaataacaga ag
//
LOCUS       DAOSOW010000412         1571 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_79291_length_1571_cov_3.486807, whole genome shotgun sequence.
ACCESSION   DAOSOW010000412 DAOSOW010000000
VERSION     DAOSOW010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79291_length_1571_cov_3.486807"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PLD67_08970"
     CDS             <1..725
                     /locus_tag="PLD67_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22696.1"
                     /translation="GLGYDFEVFINGDEVSVKLPVVGKYLRINEEMLSEGEKQYNAGN
                     KIISEETKKEFAKRWLSLMNEEDVFKGKSIVLTTPDGEVKTTEYTIKLSDEQIKTLLK
                     DTAQILSKDESLKSFFEKNISINIGKTEDELEKKSFEEILDDIISGAENFQVENFSYR
                     AYVDIDGYIVNEIIDISVKTRDSEKEGITGINYNLDIKTWDINKEQKFEFPALTDENT
                     IKPDEMNENMPSVIEDYFSIEI"
     gene            735..>1571
                     /locus_tag="PLD67_08975"
     CDS             735..>1571
                     /locus_tag="PLD67_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOT22697.1"
                     /translation="MLKVENLYKNYGNIRAVDGISFEVKRGEVFGLLGPNGAGKSTTI
                     SIISTLVPPTRGEIIFEGESILKNPKNIRQKLGVVPQDIALYPTLSGYENLRFWGNLY
                     GLKGQELKQRINETADIIGLHERLKDKVEKYSGGMKRRLNIGAALLHMPDLLIMDEPT
                     VGIDPQSRSHILDTVFKLNDRGITIIYTTHYMEEAENLCSRICIMDEGKIIASGTQQE
                     LVELVKEKTQINIKLDSINESIIDGLKNIPGIIDAKIDEDIIILYGENGDILLAQIIA
                     KVS"
BASE COUNT          599 a          213 c          332 g          427 t
ORIGIN      
        1 gaggtttggg atatgatttt gaagtcttta tcaatgggga tgaagtatcc gtaaagctcc
       61 cggttgtcgg taaataccta agaattaacg aggaaatgct ctcggaaggc gaaaaacaat
      121 ataacgctgg aaataaaata atttcagagg aaaccaagaa agaatttgct aaaagatggc
      181 tcagcctgat gaatgaagag gatgtattca aagggaaaag tatagtctta acaactcctg
      241 acggagaagt taaaaccaca gagtatacaa tcaagcttag cgatgaacaa ataaaaacat
      301 tgttgaaaga tacagctcaa atcttgtcca aggatgaatc gttaaaatct ttttttgaaa
      361 aaaatatcag tataaatata ggaaaaacag aagatgagtt agaaaaaaaa tcatttgaag
      421 aaatccttga tgacataata agcggagcag aaaattttca ggttgaaaat ttcagttaca
      481 gggcatatgt cgatattgac ggatatattg taaatgaaat tatcgatata tcagtaaaaa
      541 caagagattc cgaaaaggaa ggaataaccg gaataaatta taacctagat ataaaaacct
      601 gggatatcaa taaggagcag aaatttgaat ttcccgcact tacggatgaa aacacaatta
      661 agccggatga aatgaatgaa aatatgcctt cagttatcga agattatttc agtatagaaa
      721 tttaggggtg acatatgtta aaggttgaaa atttatataa aaattatgga aatataagag
      781 cggtagacgg tatcagtttt gaagtgaaaa gaggcgaggt gtttggtctg ttgggaccta
      841 acggtgctgg caaatctaca actatttcta ttatatcgac actggtacct cccacaaggg
      901 gcgaaataat atttgaggga gaaagtattc ttaaaaatcc caaaaatatc aggcagaaac
      961 ttggtgttgt tcctcaggat atagctcttt accccactct ttccggctat gaaaatttaa
     1021 gattttgggg caatttatac ggacttaaag gacaagagct taaacaaaga ataaatgaaa
     1081 cagcggacat tatcggattg cacgaacgct tgaaagataa ggttgaaaag tattcgggag
     1141 gtatgaaaag aagactgaat atcggggcag ctttacttca tatgcctgac ttattaataa
     1201 tggatgaacc tactgtcggt attgaccctc agtccaggtc gcatatattg gatacggtgt
     1261 ttaaactcaa tgaccgagga attacaatta tttatactac tcattacatg gaagaggctg
     1321 aaaatttgtg cagtcgcatt tgtataatgg atgaaggcaa aattattgca tccggtaccc
     1381 agcaggaatt ggttgaattg gtgaaagaaa agactcagat aaatattaaa cttgacagta
     1441 taaatgaaag tattatagat ggtttaaaaa atattcccgg tattattgat gctaaaatag
     1501 atgaggatat aataatttta tacggcgaaa acggtgatat attgttagca caaataatag
     1561 ccaaggtttc t
//
LOCUS       DAOSOW010000413         1570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_79382_length_1570_cov_2.603960, whole genome shotgun sequence.
ACCESSION   DAOSOW010000413 DAOSOW010000000
VERSION     DAOSOW010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79382_length_1570_cov_2.603960"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PLD67_08980"
     CDS             <1..724
                     /locus_tag="PLD67_08980"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008794631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HOT22698.1"
                     /translation="SKGAKVVEYDSDFSKAVEEGRKQSDQDPMSYFVDDENSMNLFLG
                     YSVAARRLKEQLNNMNLVIDETHPLFVYLPCGVGGAPGGITYGLKSVYRDNVHCFFVE
                     PTHAPSMLIGMATGLHNDISVQDLGIDGKTHADGLAVGRPSKLVGKMMDYILGGIFTI
                     DDYKLYDYIRYLHETENISIEPSACAAFEGVKLENTEEGKQYIKKHNLENKMQSAVHI
                     VWATGGSLVPEEINKQFLATRL"
     gene            938..>1570
                     /locus_tag="PLD67_08985"
     CDS             938..>1570
                     /locus_tag="PLD67_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013382301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOT22699.1"
                     /translation="MKINLNPAKGMRDITPREKEIRDYVEGVIVDTYKKSGFELFETP
                     VVENIENLTGSQGGENLMLIFKILKRGDKLDLNKEGLTEDDLSDLGLRYDLTVPLSRF
                     YCNNRSNLLPVFKALQVGNVFRAERAQKGRYRSFKQCDIDIIGDGTQAAELELIATTA
                     KALMALNVKDFVVRFNDRRVLKAVILDCGFKEDEFDGVCIVVDKLDKIGMS"
BASE COUNT          544 a          247 c          379 g          400 t
ORIGIN      
        1 atcaaaagga gcaaaggtag tggaatacga cagtgatttt tctaaagcag tggaagaagg
       61 gcgaaaacaa tcagaccaag accctatgag ttattttgtg gatgatgaaa actcaatgaa
      121 tttattttta ggctacagtg ttgcagcaag aaggctgaag gagcagttga ataacatgaa
      181 tcttgttatt gatgaaactc atcccttgtt tgtttacctt ccctgcggtg ttggaggagc
      241 tccgggagga attacttacg gtcttaaatc agtctacagg gacaatgttc actgcttttt
      301 cgtcgagccg actcatgcgc caagcatgct cataggtatg gctacagggc ttcacaatga
      361 catttccgtg caggatttgg ggatagacgg aaaaactcat gcagacggat tggctgtagg
      421 aagaccttcc aaattggtcg ggaaaatgat ggactatatt ttgggaggaa tatttacaat
      481 agatgattat aagctttacg attatattcg atatctccat gaaacagaaa acatcagtat
      541 tgagccctcg gcatgcgctg catttgaagg tgtcaaactt gaaaacacag aagaaggaaa
      601 acaatacata aagaagcaca acctagaaaa taaaatgcaa agtgcagtgc atatagtctg
      661 ggcaacaggc ggaagcctgg tgccggaaga aataaacaag caattcctcg caacccgcct
      721 atgacaaggg gacgggggta ttgtcatcat gagtgtatgg ccaaatggat ggaggtactg
      781 tcatctgaac gcagcaagta tttcattttt caaaaaaatt tgatatttaa actgctattt
      841 ggtaaaatag ttaacaatat ataaaattag aattagggac aaaaagtggg acagtcagga
      901 acgtccccac tgtcccataa ggtaggaggc agaacggatg aaaattaatt taaaccctgc
      961 aaagggaatg agagatataa cgccaagaga aaaggaaatt agagactatg tggaaggggt
     1021 aatagtcgat acgtataaaa aaagcggttt cgagctgttt gaaacacctg ttgttgaaaa
     1081 tatagaaaat ttaacaggca gccaaggcgg agaaaattta atgcttattt ttaaaatatt
     1141 aaaaagagga gataaacttg atttaaataa ggaaggatta acagaagatg acctgtccga
     1201 tttaggactt agatacgatt tgacagttcc tttaagccgt ttttattgca acaaccgctc
     1261 taatttactc cctgtattta aagctctgca ggtggggaat gtgttcaggg ctgaaagggc
     1321 tcaaaaagga agatacagaa gctttaaaca atgcgatata gatataatag gagacggtac
     1381 gcaggcagcg gaattagaac tgattgcaac gacagccaaa gctctcatgg cacttaatgt
     1441 aaaggatttt gtggttcgtt ttaatgacag gcgagtatta aaggctgtaa tactggactg
     1501 cggatttaaa gaagatgaat ttgacggagt atgcatcgta gttgacaagc tggataaaat
     1561 aggaatgagc
//
LOCUS       DAOSOW010000414         1569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_79452_length_1569_cov_3.091810, whole genome shotgun sequence.
ACCESSION   DAOSOW010000414 DAOSOW010000000
VERSION     DAOSOW010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79452_length_1569_cov_3.091810"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..918)
                     /locus_tag="PLD67_08990"
     CDS             complement(148..918)
                     /locus_tag="PLD67_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT22700.1"
                     /translation="MKKLLIILFAILLISAGCSQNEADAPATNEITESGENNNEVSKE
                     IGNSNEPEAEEDEKTEEPLSEKPSEEAEEAATEEEPSEDISEQEEPIREEVLEDNPAE
                     EETEITENALIIKGLAEKELSLTLGELKAMDDIIFEADFYSLNNFGTTGYTHFKGVKL
                     WDLLKNEALIKPEASKISIIAQDGYSMEFTIGQVQMEYMDETNPENKYPMIIAWEENG
                     EEYSLDEGNPYKLVVGQKEPGDINKPQWVSNIDVILIE"
     gene            complement(1072..>1569)
                     /locus_tag="PLD67_08995"
     CDS             complement(1072..>1569)
                     /locus_tag="PLD67_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT22701.1"
                     /translation="WAKDSIIEMAAMGYAKGSDGLFRPNDNISRAEFVSLLIRVLGMG
                     NEDAIPKFNDVKATDWFAKDVAIAAEKGYVKGYEDGTFKPNSPISRQEIGTIIGSLLE
                     DDITNAHEILSIFSDEVPEWAKVPVAKTVKAEIIKGLPGNIFGGAQNTTRAEAAVMLL
                     RYLNK"
BASE COUNT          415 a          347 c          219 g          588 t
ORIGIN      
        1 taattaatgt cactctctta gcatccggat aaaataacct cactgcttct tcttcctcgg
       61 aagaatcatt taatacataa aataatgagc ctgcaataac caaaataatt aaaataatga
      121 gaatatatct gtttttcatt ttttcaccta ttcaataagg attacatcta tatttgaaac
      181 ccattgaggt ttgttaatat ctccgggctc tttttgtcct accaccaatt tatacggatt
      241 accttcatca agactatatt cttctccgtt ttcttcccat gcaataatca ttggatattt
      301 attttcggga tttgtttcat ccatatattc catttgtacc tgcccaatag taaattccat
      361 gctgtatcca tcttgggcta taattgatat tttagaagct tcgggtttta tcaatgcttc
      421 atttttcaac aggtcccaca gcttaacgcc tttaaaatgc gtataacccg ttgtgccgaa
      481 attattcaat gaataaaaat cagcttcaaa aataatatca tccattgcct ttaattcacc
      541 taatgtaaga gatagctcct tttctgcaag ccctttaatt attagtgcat tctctgtaat
      601 ttccgtctct tcttctgccg ggttgtcttc taaaacttct tcccttatcg gttcttcttg
      661 ctcggatata tcttcggagg gttcttcttc cgttgctgcc tcttctgctt cttcagaagg
      721 tttttcactt agaggttctt cagtcttttc atcttcttct gcttccggct catttgaatt
      781 tccaatttct ttacttactt cattattatt ttcaccggat tcagtgattt catttgttgc
      841 gggagcatca gcttcatttt gagaacatcc tgctgatatc aaaagtattg caaataaaat
      901 aattaataat tttttcatat catacacctc aaatatttcc ttaaccattt atctgttaaa
      961 atatcaaaca tatttgtcat cctgagcgca agcgaaggat ctcatgagat tcttcgagct
     1021 aaagctctca gaatgacaaa attgttattc attgcaatga caaaatgttt ttcatttgtt
     1081 taaatatctt aacagcatta ccgctgcttc tgctcttgtt gtattttgtg caccgccgaa
     1141 aatatttccg ggcagccctt tgataatttc agcctttacc gtctttgcaa cgggtacctt
     1201 tgcccactcg ggaacttcat cgctgaaaat agacaaaatt tcatgagcat ttgttatatc
     1261 atcttctagc aaagagccaa ttatagtccc tatttcctgt cttgaaatag gagaattcgg
     1321 tttgaaggtg ccgtcttcat agcctttaac atatcctttt tctgctgcta ttgcaacatc
     1381 cttagcaaac caatccgtag ctttcacatc attaaattta ggtattgcat cttcattgcc
     1441 catacccagt acccttataa gcagtgatac aaattctgct ctgctgatat tatcgtttgg
     1501 tctgaataaa ccgtcgctcc cttttgcata acccattgct gccatttcta ttatgctgtc
     1561 ttttgccca
//
LOCUS       DAOSOW010000415         1566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_79634_length_1566_cov_3.682991, whole genome shotgun sequence.
ACCESSION   DAOSOW010000415 DAOSOW010000000
VERSION     DAOSOW010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79634_length_1566_cov_3.682991"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="PLD67_09000"
     CDS             <1..452
                     /locus_tag="PLD67_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOT22702.1"
                     /translation="DLEQEELTVNDRIEFSKNQVSDIIKRVWKYILIGVGIGAAIHNW
                     IPESLITAILGQDKWYSVIIAAVVGIPMYADIFGTLPIAEALIAKGVGLGTVLTFMMG
                     VTALSLPSMIMLKQVIKPRLLWTFIGLVSAGIIIIGYVFNAIGYMFI"
     gene            470..709
                     /locus_tag="PLD67_09005"
     CDS             470..709
                     /locus_tag="PLD67_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOT22703.1"
                     /translation="MIIKILGSGCKNCINLKENTEKALKETGIDAEIIKVENMKDIAS
                     YGVMQTPALVINEKVVSYGKVLKPGDIAGLLEKYK"
     gene            728..994
                     /locus_tag="PLD67_09010"
     CDS             728..994
                     /locus_tag="PLD67_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012671740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOT22704.1"
                     /translation="MKKNYEQAALLFRALSDPNRLAIIDMIKTEEKCACKILEELQIT
                     QPTLSHHMKILCDSGLVNSRREGKWMHYSINKELFEEVKQLFDF"
     gene            1029..>1566
                     /locus_tag="PLD67_09015"
     CDS             1029..>1566
                     /locus_tag="PLD67_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HOT22705.1"
                     /translation="MHIKKNNSCCSCSDGCCGSSAKPEINKREIVIDFLYLDVTVCER
                     CQGTDKSLDEAIGEVANVLAATGIDVKVNKINVLNEELAKKHKFLTSPTIRINGKDIQ
                     MDYKESLCESCGDLCGDEVDCRVWIYQGKEYSSPPKAMIIEAILKEVYGEKSEKQEKK
                     EYVIPENLRKFYKSRRSKQ"
BASE COUNT          579 a          187 c          335 g          465 t
ORIGIN      
        1 ttgatttaga acaagaagaa ttaacggtta acgataggat tgaattttca aaaaatcaag
       61 tatcggacat cataaaaaga gtatggaaat atatacttat aggtgtgggg ataggagcag
      121 caattcataa ctggattccg gagtctttaa taacagccat actggggcag gataaatggt
      181 attctgtaat aatagctgca gttgtcggta ttccaatgta tgcggatatt tttggaacac
      241 ttcccatagc tgaagctttg attgcaaagg gagtgggatt aggtaccgtt ttaaccttta
      301 tgatgggtgt aacagccctt tcattgccat ccatgattat gttaaaacaa gttataaaac
      361 ctagactctt atggacattt atcgggcttg tatctgcagg aataataata atcggatatg
      421 tatttaatgc catcggatat atgttcattt aaaatataag gagggtaata tgattattaa
      481 aatattgggt tcaggatgca aaaattgtat taatttaaag gaaaatacag aaaaagcttt
      541 aaaggaaaca ggaattgatg ctgaaataat taaagtcgaa aatatgaagg atattgcaag
      601 ctatggcgta atgcaaactc cagccttggt tattaatgaa aaggtagttt catatggaaa
      661 ggttttaaag ccgggtgata ttgcaggact attggagaaa tataaataat atttggaagg
      721 tgacagggtg aaaaaaaatt atgagcaagc agcattattg tttagggcat taagcgaccc
      781 taatcggctt gctattatag atatgattaa gactgaagaa aaatgtgcat gcaaaatatt
      841 agaagaattg caaataaccc aacctacctt atctcatcat atgaaaatat tgtgtgattc
      901 aggcttggtt aattctaggc gtgaaggtaa atggatgcat tactctatta ataaagagtt
      961 gtttgaagaa gtaaaacagt tatttgattt ctaaatttaa ttaataataa atataaaagg
     1021 ggtaccctat gcatattaaa aaaaataatt catgttgttc ttgctctgat ggttgctgcg
     1081 gttcatcagc taagcctgag attaataaga gggaaattgt tatagatttt ttgtatcttg
     1141 atgtaactgt atgtgaaagg tgtcaaggta cggataaaag tcttgacgag gctattggtg
     1201 aagtggctaa tgttcttgca gcaacaggca tagatgtaaa agtaaataaa ataaatgttc
     1261 taaatgagga gctagcaaaa aagcataaat ttttgacttc acctacaata cgcataaacg
     1321 gcaaggatat ccagatggat tataaagaaa gtctttgtga gtcctgtgga gatttatgcg
     1381 gagatgaagt tgattgccgt gtttggattt atcagggaaa agaatattca agcccgccaa
     1441 aggcaatgat aattgaagca atattaaaag aagtatatgg agaaaaatca gaaaaacagg
     1501 agaagaagga atacgtaatt cctgaaaatt taagaaaatt ctataagtct agaagatcta
     1561 aacaaa
//
LOCUS       DAOSOW010000416         1562 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_79923_length_1562_cov_3.315859, whole genome shotgun sequence.
ACCESSION   DAOSOW010000416 DAOSOW010000000
VERSION     DAOSOW010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79923_length_1562_cov_3.315859"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..344)
                     /locus_tag="PLD67_09020"
     CDS             complement(123..344)
                     /locus_tag="PLD67_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HOT22706.1"
                     /translation="MAEIKYEITEELAVLSESSRGWTKELNMVSWNDREPKFDIREWA
                     PNHERMGKGVTLNREEMKKIKDILNEIDL"
     gene            559..1485
                     /locus_tag="PLD67_09025"
     CDS             559..1485
                     /locus_tag="PLD67_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOT22707.1"
                     /translation="MKKKDILLSVLIVFLWGVNFTVIKLGLSGVPSMLLVSMRYLLST
                     FPAIFFIEKPKTEMKYIVLYGLTVGVGQFSCLFYAMQIGMPAGIASVVLQTQAFISPV
                     FAMIFLNEKLKSKQIIGFIVSAIGLIFILMASIGDGINNVPVLAFILMLAAPTFWAVS
                     NIIARIASDKAAAKGEKLNMISMVVWSGLIPPIPMFGLALMFDTPETLLGILTNLSGI
                     SIFAALYLAYGATLIGYGIWSILIASYPLNKVAPITLLVPIVALLSARIILTEQLSPL
                     QWIGFIVILTGLAISNANININKFKKDLHFGR"
BASE COUNT          496 a          265 c          253 g          548 t
ORIGIN      
        1 agacagcttt attgtaagag tactgaacgg atattaaagc gctctaaagc atacaattac
       61 aaagctcatt ctgctataga atgagctttt cctttaaaca agaaaagccg gatatatttt
      121 gactaaaggt caatttcatt tagtatatcc ttgatttttt tcatttcttc tctgtttagt
      181 gtaactcctt ttcccatcct ttcatgattc ggtgcccatt ctcttatgtc aaacttaggt
      241 tccctatcgt tccagctgac catgtttaat tcctttgtcc aacctctcga tgattcagaa
      301 agcacggcta attcttcggt tatttcatac ttaatttcag ccattttacc ctccatctat
      361 attctataat gtataataat atcaaataat atattcatga tattataccg caaatcagta
      421 ataattacta cattatatca gagaaatttc ggaatttcgg ctataattat taaaacctaa
      481 aaactaattt taatttaaaa tatatccaaa tattttaaac tatgttataa tcgaaatatc
      541 aacattagga gtaactaaat gaagaaaaag gatatattgc tgtcagtatt aatagtattt
      601 ttatggggtg tgaattttac cgttattaaa ttaggcttaa gcggagttcc gtcaatgctt
      661 ttagtttcaa tgagatattt gctttcaaca tttccggcaa tattttttat agaaaagcca
      721 aaaacagaaa tgaaatatat tgtgttatac gggctaacgg taggagtagg gcaattttca
      781 tgcttgtttt atgctatgca aatcggaatg cccgcaggca tagcttccgt tgtacttcaa
      841 actcaagctt ttatatcgcc tgtttttgcc atgatatttt taaacgaaaa gttaaaatca
      901 aagcaaatta ttggatttat agtatcagcc atagggttga tatttatatt aatggcttca
      961 atcggagacg gtattaataa cgtacctgtc ttagctttta ttctgatgtt agcggcacct
     1021 acattttggg ctgtttcaaa tataattgca agaattgctt ctgacaaagc agctgcaaag
     1081 ggtgaaaaac tgaatatgat tagtatggtt gtatggtcag gactgattcc gccaattccg
     1141 atgtttggtt tggcactcat gtttgacaca cctgaaaccc ttcttggtat attgacaaac
     1201 ttaagcggca tttcaatatt tgcagcacta tatttagctt atggtgcaac attaatcgga
     1261 tatggtattt ggagcatatt gatagcatct taccccctga acaaggtcgc cccgattacc
     1321 ctattggttc ccatagttgc attattgtcg gcaagaatta ttcttactga gcagctgtca
     1381 ccattgcagt ggataggatt catagttatt ttaacaggcc tagcaatttc taatgcaaat
     1441 attaatataa ataagtttaa aaaagatttg catttcggaa ggtaaactcg ttcaacaaaa
     1501 aacgtatata ataaaattat agctgcattc aactgaactg cagcttataa taattattca
     1561 at
//
LOCUS       DAOSOW010000417         1555 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_80432_length_1555_cov_3.986000, whole genome shotgun sequence.
ACCESSION   DAOSOW010000417 DAOSOW010000000
VERSION     DAOSOW010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80432_length_1555_cov_3.986000"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(218..1060)
                     /locus_tag="PLD67_09030"
     CDS             complement(218..1060)
                     /locus_tag="PLD67_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22708.1"
                     /translation="MRRLISILLIAMILSTGCTSLDMADENIVKDVSDIITDTVFNGA
                     GNEKAERKESHTVKSENKNTLNLMSSVGDINIVPHQSDDTIININITAKAKEKKKAEE
                     IIDNYIYKINEEGSSIVIDTTFNELPNDINLVTDLTVYLPSTIQNLEVTANVGDVNIN
                     SFIGNMDIKNNVGEVILDKSNGSYNIKADVGGIILKDCIAVGNSEFKTSTGDIELSFE
                     SISKAVSIIAETGVGNIEVSLNDDSGYHAVINEFMKDERIQTKHDQGTSINLTTGVGK
                     IEFN"
     gene            complement(1073..>1555)
                     /locus_tag="PLD67_09035"
     CDS             complement(1073..>1555)
                     /locus_tag="PLD67_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22709.1"
                     /translation="LIYFIFILINGTYIIISAGNEIDYSQIIKAVDNLLSGSLGFNLG
                     HVLVFLLAALAFLIAFITTVYTAMTIRKSIFSEIKFGGLFSFIIFLLINWVLSFLSSK
                     FYDIITPYYDTVDTISSTGRVSAGGLAMILLPMIAVLIIQAVSLTGISGYLLEKKINL
                     "
BASE COUNT          516 a          261 c          202 g          576 t
ORIGIN      
        1 tgatggaata aataaatagg taaatcattc tttttcataa aagccccggt aaaatttatt
       61 tgttcttatt taaagtatac gacaaaataa ataactttta aagatataat ttataaacat
      121 aagtacttat aatataagta taccttgctt tagacaagtt taaacaaaaa agacgacaaa
      181 aaaagaacac tcatatgaat gcccttttat ctaataatca attaaattct attttgccta
      241 cacctgtggt aagattaatg ctggtacctt ggtcatgttt tgtttggatt ctttcatctt
      301 tcataaattc attgataacc gcatgatatc ccgagtcgtc atttagcgat acttcaatat
      361 ttccaactcc tgtttctgct ataatgctca ctgcttttga aatgctttca aaggacaatt
      421 ctatatctcc ggtacttgtt ttaaattcag aatttcctac tgctatgcag tctttgagaa
      481 taattccccc aacgtcggct tttatattat atgaaccatt tgatttatct aaaataactt
      541 caccaacatt attttttata tccatgttcc cgataaaact gtttatgttt acatcaccga
      601 catttgctgt aacttctaaa ttttgtatgg tagaaggaag atatacggtt aaatcggtta
      661 caaggtttat atcattaggg agctcattaa atgttgtatc aataactatg gagcttcctt
      721 cttcattaat tttgtaaatg taattatcga taatttcttc agcttttttc ttttcttttg
      781 cttttgctgt aatatttata tttattattg tatcatcaga ttggtgcggt actatattta
      841 tatctccaac agagctcatt aagtttagag tatttttatt ttctgattta acagtgtgag
      901 attcctttct ttctgctttt tcatttcccg caccgttaaa tactgtgtct gtaataatgt
      961 cggagacatc cttaactata ttttcatcag ccatatctaa tgaagtgcag cctgtagaca
     1021 gaatcatagc tattaataat atactaatta atcttctcat atttccccct gtttataagt
     1081 taattttctt ttctaaaaga tatcctgaaa ttcctgttaa agaaacagcc tgaattatta
     1141 atacggcaat cattggaagc aatatcatag ctaatcctcc ggctgaaact ctgcctgtac
     1201 ttgatatagt atcaacggta tcataatagg gagtaataat atcatagaat ttacttgata
     1261 aaaatgaaag tacccaatta atcaataaaa atattatgaa actaaacagc cccccaaatt
     1321 taatttcgga aaatatactt tttcttattg tcatagctgt ataaaccgtt gtaataaatg
     1381 ctattaaaaa tgcaagtgca gccagcaaaa atactaatac gtggcccaag ttgaaaccaa
     1441 gactgccgga caataaattg tcaacagctt ttattatttg actgtaatca atttcattac
     1501 ccgctgaaat aataatatat gttccgttta tcagtataaa tataaaatat attaa
//
LOCUS       DAOSOW010000418         1553 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_80604_length_1553_cov_3.002003, whole genome shotgun sequence.
ACCESSION   DAOSOW010000418 DAOSOW010000000
VERSION     DAOSOW010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80604_length_1553_cov_3.002003"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..1505
                     /gene="pyk"
                     /locus_tag="PLD67_09040"
                     /pseudo
     CDS             90..1505
                     /gene="pyk"
                     /locus_tag="PLD67_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311193.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
BASE COUNT          579 a          262 c          322 g          390 t
ORIGIN      
        1 cgggacactg gggacggtcc tttttgtccc acttcacatt tgtttatggt gccaggcacc
       61 atacgaggga gatgaaatat atgctgaata tgcgcaaaac taaaatagta tgtaccattg
      121 gtccttcatc tcaagatgaa gaaacattca aggaattggt tataaacggt ttaaatgtgg
      181 caagattgaa cttttcccac ggtacacatg aagaacataa agaaaaaata aatactataa
      241 aaaaggtgcg agaagaacta aatacctcta tagcgataat gcttgatacc aaaggtcctg
      301 aaatacgaat cagagatttt atagacggtc aagttgaatt aaaaaagggt caggaattta
      361 ttcttacaac aagggagctc caaggaaatg actctattgt cagtgtcacc tacgaccaat
      421 ttgcaaatga cataaaaaaa ggtgatacga tactcataga cgacgggctg ataagtatgg
      481 aagcagttga aattatcaat aatactgata taaaatgcct tgtaaaaaac ggcggtatta
      541 ttaaagacaa aaaaggcatc aatgtacccg atgttcaaat taatttacct gcacttaccg
      601 ataaggatgt aagcgacatt aaattcggaa tagagcaaga aatcgactat atagcagcat
      661 catttatcag aaaggctgat gatgttattc aaataagaaa tattttagaa aaagaaaatg
      721 cagctcacat aatgattatt tcaaaaatag aaaacaggca gggcgttgaa aacatagatg
      781 aaataattga ggcttcggat ggaataatgg ttgcccgggg agatttaggt gtggaaatac
      841 cggcagaaga agtcccgctc gtacaaaaaa tgattatcaa aaaatgcaat caggcaggaa
      901 agcccgtcat tactgcaacc caaatgctcg attcaatgat tagaaatccg agacctacga
      961 gagcagaagt atcagatgtg gcaacagcaa tatttgaagg tacagatgcc ataatgcttt
     1021 caggtgaaac tgcttcaggc tgctaccctc ttgaagcggt aaaaacaatg gcaagaatat
     1081 ctcttatgat tgaaagctca ttaaactatg gcgagctttt ggaaagaaaa aggctgggct
     1141 caacttacag cataaccgac tctatcagct atgcagcctg caagtcttgt ttagatttaa
     1201 atgcatcagc aataatttcc gctacatcgt caggctatac agctgcaaat gtttcaagat
     1261 acagacccgt tgcacccata attgcagcta cacaaagcga tagggttatg aggaaactgg
     1321 ctctttattg gggagtatat acgataaaga taggaaaaat gcagtctacg gaccttataa
     1381 ttgaaaaatc agtagaaact gcactaaaat taggatattt aaaaaaggga gatatagcaa
     1441 taataactgc aggaataccc gtaggagttt ccggcattac aaatttatta aaggtgcata
     1501 ttgtcaatta gcgtaaagct gaaatccaat aatcaagatg gagtttcagc tga
//
LOCUS       DAOSOW010000419         1552 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_80676_length_1552_cov_3.130929, whole genome shotgun sequence.
ACCESSION   DAOSOW010000419 DAOSOW010000000
VERSION     DAOSOW010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80676_length_1552_cov_3.130929"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLD67_09045"
     CDS             <1..487
                     /locus_tag="PLD67_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOT22710.1"
                     /translation="TIRDLVRNSLRMRPDRIIVGEVRAGEAIDMLQAMNTGHDGSLTT
                     AHANSPRELMSRLETMVLMSGMDLPVRAIREQIHSAIDLVVHQARMKDGSRKVVNITE
                     VVGMEGDTITLQDIFVYKVEGVDSNGRMKGYFTSTGIRPNFLDKLSSSGIIVRDDWFV
                     N"
     gene            496..1440
                     /locus_tag="PLD67_09050"
     CDS             496..1440
                     /locus_tag="PLD67_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOT22711.1"
                     /translation="MKILISLMVALFIYLCFNILISSYGPGQARSRIKRYFNEYSVDD
                     VQEQFIREKSEEESKRKKDRFKIASKEFSNLIASSGIRLKANEFLYFWAGLTLIPMMV
                     VLLFSGNIITGAALSIIGISIPPFIVSSARKKREELFTKQLGDATIIMGNSIKGGFTF
                     LQSMENIAEEMQPPISTEFKKVLREIHYGVKQEDALNHMVERTKNKDLELLISAVMTS
                     AQVGSNLSDILDTISNTIRDRIRIKNEIKTLTAQGRMSGLVIGLLPIVLILAIMVLTP
                     SYFEGFFDSTIGKILIAVSVLLEITGFAIINKIINIKV"
BASE COUNT          519 a          242 c          350 g          441 t
ORIGIN      
        1 aacaataagg gatttggtta gaaactcttt gcgtatgcgt ccggacagga taatagtcgg
       61 agaggtacgt gccggtgaag caatagacat gctgcaggcg atgaatacag gtcatgacgg
      121 ctcactgaca acagcccatg ccaattctcc gagagagctt atgtcccgtt tggaaacaat
      181 ggtattaatg tccggaatgg acttgcccgt cagggcaata agggagcaaa tacattccgc
      241 aatcgatttg gtggttcatc aggcaagaat gaaagacggc tcaagaaagg tagtaaatat
      301 caccgaggtt gtaggtatgg agggggatac aattacattg caggatattt ttgtatacaa
      361 ggttgagggt gttgacagca atggaagaat gaaagggtat tttacgtcaa caggtatacg
      421 gccgaatttt ctcgataaac tgtcatcaag cggcataata gtcagagatg actggtttgt
      481 aaattgaggt gaaatatgaa aattctaata tctcttatgg ttgctttgtt tatttatttg
      541 tgtttcaata ttttgatatc ctcatatggg ccgggacaag ccaggagcag gataaaaagg
      601 tattttaacg aatatagcgt tgatgatgtt caggagcaat tcatcagaga aaaaagtgaa
      661 gaagaaagca aaagaaaaaa ggatagattt aaaatagcaa gcaaggaatt ttcaaacctt
      721 attgcttcct cgggcataag gctgaaggcc aatgaattct tatatttttg ggcaggcttg
      781 acattaatac cgatgatggt ggttttactt ttcagcggaa acataattac gggagcagcc
      841 ctgtccatta ttggcatttc aataccgccc ttcatagtaa gcagcgcgag aaaaaagcgc
      901 gaggaacttt ttaccaagca attgggggat gcaaccataa taatgggaaa cagcattaaa
      961 ggcggtttta catttttgca gtccatggaa aatattgcag aagaaatgca gccgcccata
     1021 tccactgaat ttaaaaaagt gctaagagaa atacattatg gcgtcaagca ggaagacgca
     1081 ctcaatcaca tggttgaaag gacaaaaaat aaagatttgg agcttttgat atccgcagta
     1141 atgacttcgg cacaagtcgg aagcaattta tccgatattc ttgatacaat ttcaaataca
     1201 ataagagacc gcatcaggat aaaaaatgaa atcaagacat tgacagccca aggacgaatg
     1261 tcgggactgg ttataggact gctcccgata gtgcttattt tagccataat ggttttgact
     1321 cctagttatt ttgaaggatt ttttgattca accattggaa aaattttaat tgccgtatct
     1381 gttttattgg aaattacggg gtttgcaatt attaataaaa taataaatat aaaagtgtga
     1441 ggtatatatg actctttata tttcggttgt ttttgcaatt ttcatattca gtttaattat
     1501 tattatatta aacaaatatg gtttgaaccg cgataaaatt aataacagac tg
//
LOCUS       DAOSOW010000420         1544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_81288_length_1544_cov_3.214909, whole genome shotgun sequence.
ACCESSION   DAOSOW010000420 DAOSOW010000000
VERSION     DAOSOW010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81288_length_1544_cov_3.214909"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="PLD67_09055"
     CDS             <1..417
                     /locus_tag="PLD67_09055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PduL/EutD family phosphate acyltransferase"
                     /protein_id="HOT22712.1"
                     /translation="KRTIKGVRILGPARKETQVELSFSDGFTLGITDLPVRDSGKTEG
                     TPGLKLVGPKGEVEIERGAIAAARHLHMHTTDAEKYGLKDKDIVSVKVDGQRGLVFDN
                     VLVRVNEAYALDMHVDIEEGNAAGLKNGDLVEVIVE"
     gene            706..>1544
                     /locus_tag="PLD67_09060"
     CDS             706..>1544
                     /locus_tag="PLD67_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOT22713.1"
                     /translation="MDFLPVNMEDMKKRGWDECDFVFVTGDAYVDHSSFAAAVLSRTL
                     EKFGYKVGIIPQPDWKELSSFKVLGKPRLAFLVNSGNIDSMVNHYTTFKKRRHQDSYS
                     PGGKAGLRPDRAVTVYSNRIREAYKDVPVIIGGIEASLRRFSHYDYWSDSVKRSVLLD
                     SGADLLVYGMGEKVLIEIAEALESGIPVEEITFINQTVYKTKDRERPYNPIFLPDYNE
                     VVSSKKKYGESFKIQYENTDSKSARTLVEPYNTVYVVQNPPMEILSQHELDDIYDMDY
                     MRD"
BASE COUNT          516 a          206 c          347 g          475 t
ORIGIN      
        1 aaaagaacaa tcaaaggtgt taggattctg ggacctgcaa gaaaagaaac acaggttgaa
       61 ttatcttttt ctgatggatt tacattaggt attactgacc ttcctgtgag agattcagga
      121 aaaacagaag gtactccggg tcttaaactt gtgggaccga agggtgaagt tgagattgaa
      181 agaggtgcta ttgctgcagc aagacattta catatgcata caacagatgc tgaaaaatac
      241 ggccttaaag ataaagatat agtttctgtt aaagtagatg gtcagagggg tctggttttc
      301 gataatgtat tagtaagagt aaatgaagca tatgcattag acatgcatgt tgacattgaa
      361 gaaggaaatg ctgccggact gaaaaacggg gatttagtag aagtaatagt ggaatagttt
      421 acataaaata atttatgtaa actccgaccg ttaattctgt gcaagtattt ggttctatat
      481 agtttgttgg atacatgaag atatataaca attttgctct atgtaagtga attggataca
      541 tggtagatgt ttaaatgtct gaacagagtg ttattattta gttctatata gttattgaat
      601 acatgttttt atataaaagc agaggagtaa ttcttctgct tttattattg ttttaaatat
      661 aatgcaggtg gtataataaa cacattaaca ttatatggag gcattatgga ttttttaccc
      721 gttaatatgg aagatatgaa aaaaagagga tgggatgagt gtgattttgt ctttgtgaca
      781 ggtgatgcat atgttgacca ttcttcattt gccgctgctg ttttgtcaag aacattggaa
      841 aaattcggtt acaaggtcgg aatcattccg cagcctgact ggaaagaatt atcttctttt
      901 aaagtgttgg ggaagccgcg cttagccttt cttgttaatt caggcaatat agattcaatg
      961 gttaatcatt atacgacttt taagaaaagg agacatcaag attcatattc tccgggcggg
     1021 aaagcaggat taagacctga cagagctgtt acggtatatt caaaccgtat aagagaagca
     1081 tacaaggatg ttcccgtaat aataggcggt attgaagcga gcttaagaag atttagccat
     1141 tatgactatt ggagcgacag cgttaaaagg tctgttttat tggattctgg tgcggattta
     1201 ttggtatacg gcatgggtga aaaagttttg atagaaattg cggaagcatt ggaatccggt
     1261 attccggttg aagaaattac atttataaac caaaccgtat acaagacaaa ggacagagag
     1321 cgtccctaca atccgatatt tttgcctgac tataatgaag ttgtatcttc aaagaaaaag
     1381 tatggtgaaa gctttaaaat acaatatgaa aataccgact ccaaaagtgc gaggacctta
     1441 gttgagccgt ataatacagt ctatgtggta caaaatcccc ctatggaaat tctatcacag
     1501 catgaattag atgatattta tgacatggat tatatgaggg atta
//
LOCUS       DAOSOW010000421         1541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_81512_length_1541_cov_2.714670, whole genome shotgun sequence.
ACCESSION   DAOSOW010000421 DAOSOW010000000
VERSION     DAOSOW010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81512_length_1541_cov_2.714670"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1541
                     /locus_tag="PLD67_09065"
     CDS             <1..>1541
                     /locus_tag="PLD67_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HOT22714.1"
                     /translation="RTSDAIKKLIDLSPKTALVERLGIEMEIPVEDVVKGDIIIVKPG
                     QSIPVDGVIVFGSSSVDESMLTGESIPVEKKVGDKVVGASINKSGFFKFEAQKVGDDT
                     TLAQIIKLVEEASSSKAPISKMADKISGIFVPIVIVIAVLATIVWLILGADFEFALSI
                     GIAVLVISCPCALGLATPTAIMVGTGKGAENGILIKSAEALEIAHHVQTVVMDKTGTI
                     TEGKPRVTDLVADGIDEKEMLRIAASAEKGSEHPLADAIVEEAGKLNLELYDVENFEA
                     IPGQGLRAVINNKKYYLGNLRLVNEQNINIKNFEEISTKFADEGKTPLYFADENNVLG
                     IISVADIVKPTSQAAIKEFEAMGIEVVMLTGDNKKTAEAIRKQLGITRAVAEVLPQDK
                     EKEIRKIQEQGRKVAMIGDGINDAPALARADVGIAIGAGTDVAIESADIVLIRSDLLD
                     AVTAVQLSKATIKNIKQNLFWAFIYNTIGIPLAAGVFYGISGWKLNPMFAGAAMSLSS
                     VSVVS"
BASE COUNT          516 a          236 c          376 g          413 t
ORIGIN      
        1 ggaggacttc cgatgccata aaaaaactca ttgatttatc cccaaagact gctttggttg
       61 aaagattagg aattgaaatg gaaatacccg ttgaagatgt ggtaaaggga gatattataa
      121 ttgttaagcc aggacaatcg atacctgttg acggtgtgat tgtattcggt tcttcatcag
      181 tagatgaatc aatgcttaca ggtgaaagta tacctgttga aaagaaggtc ggagataagg
      241 tagtaggtgc aagtattaat aaatcaggtt tctttaaatt tgaagcacag aaggtaggag
      301 atgatactac attagctcaa ataattaagc tggttgagga agcaagctct tcaaaggctc
      361 ccatatcaaa gatggctgat aaaataagcg gcatatttgt tccgatagta attgtgatag
      421 ccgtcttggc aacaatagta tggctgatat tgggagcaga ttttgaattt gctctttcaa
      481 taggaatagc agtcttggtt atttcttgtc cttgtgcact aggccttgca acgccaactg
      541 ctataatggt cggtaccggg aagggtgctg aaaacggaat tcttattaaa tcagctgaag
      601 cattggaaat agctcatcat gtgcagacgg tggtaatgga taaaacagga accataacag
      661 aaggtaagcc cagggttacc gaccttgttg ccgatggaat cgatgaaaaa gaaatgttaa
      721 gaatagcggc ttctgcagaa aagggctccg agcatccttt agctgatgca atagttgaag
      781 aagccggtaa gttaaattta gagctgtacg atgtagaaaa tttcgaagca attcccggtc
      841 aagggttaag agctgtaatt aacaataaaa aatactatct tggtaacttg cgtttagtaa
      901 atgaacaaaa tataaatatt aagaactttg aagaaataag cactaaattt gcagatgaag
      961 gaaaaactcc tttatacttt gcagatgaaa ataatgttct gggtataata tcagtggctg
     1021 atattgttaa acctaccagc caggctgcaa ttaaagaatt tgaagcaatg ggaatagagg
     1081 ttgtaatgct taccggagac aacaaaaaaa ctgccgaagc aataagaaag cagctgggaa
     1141 ttacgcgagc tgttgctgaa gtactgccgc aggataaaga aaaagaaatc aggaaaattc
     1201 aggagcaggg cagaaaggtt gcaatgatag gcgacggtat aaatgatgct ccggcacttg
     1261 ccagagccga tgtgggaatc gccataggtg caggaacgga tgttgcaata gaatcggcag
     1321 atatagtgct tataagaagt gatttgctgg atgctgttac tgcagtccag ttgagcaagg
     1381 ctacaataaa aaatattaaa caaaatttat tctgggcatt tatttacaat acaataggta
     1441 tacctctggc agcaggtgta ttttacggta tatcaggatg gaagctgaat cctatgtttg
     1501 cgggagctgc catgagtttg agctcggttt cagtggtttc t
//
LOCUS       DAOSOW010000422         1526 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_82656_length_1526_cov_2.602991, whole genome shotgun sequence.
ACCESSION   DAOSOW010000422 DAOSOW010000000
VERSION     DAOSOW010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82656_length_1526_cov_2.602991"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..378)
                     /locus_tag="PLD67_09070"
     CDS             complement(103..378)
                     /locus_tag="PLD67_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HOT22715.1"
                     /translation="MPRRDGTGPIGMGPMTGRGFGFCGHRFYGRRNCFGRGFGPWYGA
                     RYGFAPGYGPGYLRQDITEKEFLKSQKEFLESQIELLEKELEDLKDD"
     gene            complement(653..>1526)
                     /locus_tag="PLD67_09075"
     CDS             complement(653..>1526)
                     /locus_tag="PLD67_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOT22716.1"
                     /translation="RTNSLEEALKLQNKGYLVYPDPSDEKVAENFKNGKGPIFEKIGK
                     LPIWNEEILKNRIAHLRELGAERICFKTGPFDPKDLIRILKIASENEVDLVTFDGAGG
                     GTGNSPVKMMNEWGMPTVYLESMLYDILKRMDKKGYFLPQVAITGGFTLEDHIFKGLA
                     LGAPYIQFVAVGRAAMAAAMVGKQVGELIEAGNIPKEYQSFGSTKEEIFADIRELKLY
                     YDNAQDLPTGAVGVYSYISRIAAGIKQLMALNRKFDIKYIDRSDIFPLTDLAAQVTGL
                     DTYEDVYVRELEKL"
BASE COUNT          407 a          335 c          214 g          570 t
ORIGIN      
        1 tagatacgga aatacggtgc acgaccaaaa taatcataaa tatttgattt aatagacttt
       61 tcttctgttg ggattgctat tttcatatta tctccaacct ttctaatcgt cttttaaatc
      121 ttccaattct ttttcaagta gctctatttg tgattccaag aattcttttt ggcttttcaa
      181 aaattctttt tctgtgatat cttgtcttaa atatcccggt ccatatcccg gagcaaatcc
      241 ataccttgca ccataccagg gtccaaaacc tcttccaaag caatttcttc tgccataaaa
      301 cctatgaccg cagaatccaa aacctcttcc tgtcattggt cccattccta taggacccgt
      361 tccatctcta cgtggcatct ttatcctcct ttcattttcg acatatgtca tttatcttat
      421 attatacact atatccgaca tatgtcaata acttttttaa aaaaactgca aagaaatttt
      481 tgcagtttta attattttat atgaatacat gttagtgtct tgcaatgatt aacattttta
      541 tcattgctat gaataagcta tgtgtcattc tgagagctta agctcgaaga atctcatttt
      601 ttaaaccctg agatccttcg cttacgctca ggatgacaaa tggtatttgt tcctacagtt
      661 tttctaattc tcttacataa acatcttcat atgtatctaa gcctgttact tgtgctgcta
      721 aatcagtcaa tgggaagatg tcgcttctgt ctatatattt tatgtcaaat ttacggttta
      781 aagccattaa ttgctttatt cctgcagcta ttctgcttat atatgaatac actcctacgg
      841 ctcctgtcgg taagtcttgt gcattgtcat aataaagctt taattctctt atgtcagcaa
      901 aaatttcttc ctttgtgctt ccgaaacttt gatattcttt aggtatattg cctgcttcaa
      961 tcaattcgcc cacttgtttt cctaccatgg ccgcagccat tgcggctctt ccgacagcta
     1021 caaattgaat atatggagct cctagagcca atcccttaaa tatgtggtct tctaaagtaa
     1081 atcctcccgt aattgcaact tgaggcaaga aatatccttt cttatccatt cgtttcagta
     1141 tatcatacag catactttcc aaataaacgg taggcattcc ccattcattc atcattttaa
     1201 cggggctgtt ccctgtgcct cctccggcac cgtcaaatgt aaccaaatcc acttcatttt
     1261 ctgatgcaat ttttaaaatc ctgattaagt cttttgggtc gaagggacct gttttaaagc
     1321 aaatcctttc tgctcccaat tctcttaaat gtgcaatgcg gtttttgaga atttcttcat
     1381 tccatatagg aagcttcccg attttttcaa atataggtcc ttttccattt ttgaaatttt
     1441 cagcaacttt ttcgtccgat gggtcaggat acaccaaata tcctttattt tgaagtttta
     1501 gagcttcctc taatgaatta gtcctt
//
LOCUS       DAOSOW010000423         1480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_86296_length_1480_cov_4.115088, whole genome shotgun sequence.
ACCESSION   DAOSOW010000423 DAOSOW010000000
VERSION     DAOSOW010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86296_length_1480_cov_4.115088"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1480)
                     /gene="uvrC"
                     /locus_tag="PLD67_09080"
     CDS             complement(<1..>1480)
                     /gene="uvrC"
                     /locus_tag="PLD67_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOT22717.1"
                     /translation="MKNAFGEIIYVGKAKNLKKRVRQYFQSKNHIPKIQAMIKNIYEF
                     EYIITDNEVEALILEANYIKKYRPKYNTLLRDDKQYPYIKISVQERYPRIYKVREVKK
                     DGAKYFGPYPSAYAVNEIIDIIGNLYKLRKCSLKLDGTKKSQRPCVYYHINRCTAPCS
                     GNVDIKEYSQEVNKATALLTGSEGIIKSLNEKMIEASENMEYEEAAKYRDQIKALEVI
                     SEKQKVVSQAGIDQDVIGSAIGIDEACIQIFFIRNGKIIGSEHYLLTNIENETREQII
                     SSFLTQFYTGTVYIPKEIYIETKIEDMEVFEKLLSQKRGNKVTLKVPVKGEKSMLIKM
                     IKKNALEILEKGSRRIKKNIDEGMEAIKELSNVLNLDKTPERIEAYDISNIQGVESVG
                     SMVVFENGIPNKSNYRRFKIKTVEGPNDYKSLEEVLERRINRGLGDGKQGFNKMPDLI
                     LIDGGKGQTNIAEEVISNFGLSIPVCGMIKDDKHTTKGLIYK"
BASE COUNT          383 a          291 c          188 g          618 t
ORIGIN      
        1 tttttatata ttaatccttt agttgtatgt ttgtcatcct taatcatacc gcaaacagga
       61 atacttaaac caaaattact tattacttct tctgcaatat ttgtttgtcc ctttccgccg
      121 tcaattaaaa ttaagtcggg cattttgttg aaaccttgct tgccatctcc taaccctctg
      181 ttaattcttc tttccaatac ttcttccaaa cttttatagt catttggtcc ctctactgtc
      241 tttattttga aacgacggta attgctttta ttgggaattc cgttttcaaa aacaaccatg
      301 gagcctacag attcaactcc ttgtatattc gaaatatcat aagcttcaat cctttcggga
      361 gttttatcca aatttaaaac attactcagc tctttgattg cctccatgcc ttcatcaata
      421 ttcttcttta ttctcctgct tcccttttct aatatttcaa gagcattttt ctttatcatt
      481 ttaataagca tgctcttttc gcctttaacc ggaactttta aagtaacctt gtttcctctt
      541 ttttgggaca agagtttttc aaaaacctcc atatcttcta ttttggtttc tatatatatc
      601 tcttttggaa tatatactgt acctgtatag aattgtgtta aaaatgagct gataatttgt
      661 tcccttgttt cattttctat attcgtaagc aaataatgct cgctgcctat aattttaccg
      721 tttcgaatga agaaaatctg tatgcaggcc tcatcaatac caattgcgct tcctataaca
      781 tcttggtcta ttcctgcttg agaaacaact ttttgttttt ctgaaattac ttcaagagcc
      841 ttaatctggt ctctatattt ggcagcttct tcgtattcca tattttctga agcttcaatc
      901 attttttcgt ttaacgattt aataatgcct tcactgcctg tcaatagagc tgttgcttta
      961 tttacttctt ggctgtattc ttttatgtca acgtttccac tacagggtgc ggtacacctg
     1021 tttatatgat aatatacgca tggtctttgg cttttctttg taccgtcaag ttttaaagag
     1081 cattttctaa gcttgtataa gtttcctata atatctatta tttcatttac ggcataagca
     1141 ctcggatagg gtccgaaata ttttgcccca tcttttttta cttctctgac cttataaatt
     1201 cttgggtatc tttcttgaac agatatttta atatacggat attgcttatc atctcttaaa
     1261 agagtattgt atttaggtct gtattttttt atataatttg cttccagaat cagagcttca
     1321 acttcattgt ccgtaataat atattcaaac tcataaatat tttttatcat agcctgaatt
     1381 ttaggtatat gatttttaga ctgaaaatat tgtctgaccc tcttttttaa atttttagcc
     1441 ttgcccacat atattatttc accaaaagca tttttcatct
//
LOCUS       DAOSOW010000424         1462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_87913_length_1462_cov_2.696517, whole genome shotgun sequence.
ACCESSION   DAOSOW010000424 DAOSOW010000000
VERSION     DAOSOW010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87913_length_1462_cov_2.696517"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..532)
                     /locus_tag="PLD67_09085"
     CDS             complement(74..532)
                     /locus_tag="PLD67_09085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOT22718.1"
                     /translation="MSKNEDLISIIMPAYNCEKYVEEAINSVLAQSYRNWELIVIDDG
                     SVDNTRNIITEYLQNDFRISLLSNEKNIGVSAARNRGIDFAKGKWIAFLDSDDMWTPY
                     KLEKQIEAVEENNAEFIFTGSAYINEEGKPYKGIFEVPEKVSYKRLKSRM"
     gene            complement(622..>1462)
                     /locus_tag="PLD67_09090"
     CDS             complement(622..>1462)
                     /locus_tag="PLD67_09090"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOT22719.1"
                     /translation="QLNGFVNKVFASGLIEGSVSALNYSMSVKTFIQSVFSVAITTMI
                     YPMLSQSIAENDINAVKKMVSNAVNIIIILFIPITVGVVLLSEPAIFLVYERGMFSSE
                     STAMTSIALQMYSLSLVAVCLIDVLTKMFYAMKDAKTILYLNTFTVAICIASSIILVK
                     PMMHAGLALAGSLAEIITLPLFFFFLKRRIGNLGMKNSFNILVKSCICSAIMGITVCF
                     VYNYSYYALSTDKLYILLSILISVAAGTLTYFVLMNLMKVKEMDFFTYIIKDIFRKAF
                     GKI"
BASE COUNT          477 a          287 c          196 g          502 t
ORIGIN      
        1 tcatgcatgt cattcccttc cattttaata ttaataaaat attttttatt aataagaaca
       61 gatgagcatg atattacatt ctgcttttaa gcctcttata tgataccttt tcaggaacct
      121 caaatatacc tttatatggt ttaccttctt cattgatata agctgaaccc gtaaatatga
      181 attccgcatt gttttcttct acagcttcaa tctgcttttc cagcttgtat ggagtccaca
      241 tgtcatcact gtctaaaaat gctatccatt ttccttttgc aaaatctatc cctctattcc
      301 ttgctgcgga aaccccaata tttttttcat tggataataa gcttattcta aaatcatttt
      361 gtaaatattc ggttataatg ttacgggtat tatctacgga gccatcatca ataactatga
      421 gctcccaatt tctatagctc tgagcaagta ctgaatttat tgcttcttca acatattttt
      481 cacaattata cgcaggcatt attatgctga ttaaatcttc atttttgctc attacctttt
      541 ccgccccctt tattttaatg tattaacgta attcaatttt actttttaat cattatcccc
      601 cttgcttgct gtataaaatc gttaaatctt tccgaatgcc ttccgaaata tgtctttaat
      661 tatataagta aaaaagtcca tctccttaac cttcataaga ttcattaaaa caaagtatgt
      721 caatgtccct gcagcaactg aaattaaaat cgataaaagt atataaagct tgtctgtgct
      781 tagtgcataa tatgaataat tatatacaaa acatactgtt atacccatta ttgcagagca
      841 tatacatgat ttaacaagta tattaaaact atttttcatt ccaagatttc cgatgcgcct
      901 ctttaaaaag aagaaaaaca aaggaagtgt tataatctcc gccaagctgc ctgcaagagc
      961 aagccctgca tgcatcattg gtttaaccag gattatactt gatgcaatgc atattgcaac
     1021 cgtgaatgta ttcaaataca atatcgtttt cgcatccttc atggcataaa acatcttagt
     1081 taaaacatct atcaggcaaa cagcaaccag gcttagagaa tacatctgta aagcaataga
     1141 ggtcatggct gtcgattcac tgctgaacat tcccctctca tacacaagaa atattgccgg
     1201 ttccgacagt aaaacaacac ctacggttat cggaataaat aatattatta tgatatttac
     1261 agcatttgag accatttttt taacagcatt aatatcattt tctgcaatgc tttgtgaaag
     1321 catggggtaa atcatagtag taattgcaac agaaaaaaca ctttgaatga atgtcttgac
     1381 agacatggaa taattaagtg ccgaaacact gccctcaatt agtccggatg caaatacctt
     1441 gtttacaaaa ccatttaatt ga
//
LOCUS       DAOSOW010000425         1460 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_88089_length_1460_cov_3.479004, whole genome shotgun sequence.
ACCESSION   DAOSOW010000425 DAOSOW010000000
VERSION     DAOSOW010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88089_length_1460_cov_3.479004"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLD67_09095"
     CDS             <1..199
                     /locus_tag="PLD67_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HOT22720.1"
                     /translation="DNYGADSYIGRSASIIMGSSETIFYITAVYFGSVGISDYRYTIK
                     AGLLSHAATIFAALLVCKLMF"
     gene            complement(188..772)
                     /locus_tag="PLD67_09100"
     CDS             complement(188..772)
                     /locus_tag="PLD67_09100"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HOT22721.1"
                     /translation="MYFEIIFFSLLSIITAIVVLLIKKRDFDFDRYNIKIRGYKVLIL
                     AAVIKIAAEFLFNKYTNVTLLRILSHNWVIYFGLLYISLLNIKRSYMMLFFLGALFNF
                     AAIAANDFKMPVYVSELLSDVETKKMFLLSGRDLVHSLLTKETKLKFLCDIITLYPPY
                     PFPKTISVGDVFLLSGIFAFWQDLFYESSKILKH"
     gene            complement(762..>1460)
                     /locus_tag="PLD67_09105"
     CDS             complement(762..>1460)
                     /locus_tag="PLD67_09105"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOT22722.1"
                     /translation="LAFAYYSYGSGGLLLFFIPLLLARYTFKLYLDMRKNYFETLNVL
                     VRAIEASDPYTSGHSMRVSAYAEAIARQVGLPQSKIDLIKSAALLHDIGKIGIDKNIL
                     NKTGKLKKEEFEKIKSHPEIGATIIADLSYLSNITDIIRHHHERNDGKGYPDGLCHSN
                     ISLETSILTIADSFDAMTTDRPYRSALSLESALQEIKNNEGTQFNPDIVDDAIIALKK
                     AFFKIAEEKVEHVF"
BASE COUNT          472 a          259 c          241 g          488 t
ORIGIN      
        1 agataattac ggagctgatt catatatagg aagaagcgca tcaataatta tgggctcatc
       61 ggaaacaata ttctatataa ccgcagtata tttcggctcg gtcggtattt ccgattacag
      121 atatacaata aaggcggggc ttctatcaca tgcagcaaca atctttgctg ccctgcttgt
      181 atgtaaatta atgttttaaa atctttgagc tctcataaaa caagtcttgc caaaatgcaa
      241 aaatcccgga taataaaaac acatcgccga cacttatagt ctttggaaaa ggatagggag
      301 gatataatgt aattatatca caaagaaatt ttaattttgt ttctttcgtc aatagtgaat
      361 gcactaaatc ccttccggaa agaagaaaca ttttttttgt ttccacatct gaaagcagct
      421 cagatacata aacaggcatt ttaaaatcat ttgctgcaat tgccgcaaaa ttaaataagg
      481 cacctaaaaa aaacaacatc atgtaagacc ttttaatgtt tagcaatgaa atataaagaa
      541 gtccaaaata aatgacccag ttatgagata aaattctaag caaggttacg tttgtgtatt
      601 tgttaaacaa aaattcagca gcaattttga ttactgccgc caatattaat accttatagc
      661 ctctgatttt tatattgtat ctgtcaaaat caaagtctct tttttttatc agtaaaacta
      721 caattgctgt tattattgat agtaaggaaa aaaatataat ttcaaaatac atgttcaacc
      781 ttctcttcag ctattttaaa gaatgctttc ttcaaagcga ttatcgcatc atctacaata
      841 tcagggttaa attgtgtacc ctcattattt ttgatttctt gaagagcaga ttcaagactt
      901 aaagcacttc tgtaaggtct gtctgttgtc atagcatcaa aggaatcggc aatggtcagt
      961 atggacgttt ctaatgatat attggaatgg cataatccgt cagggtagcc ctttccgtca
     1021 tttctttcat ggtgatgtcg tattatatct gtaatatttg ataaatagct taaatctgca
     1081 attattgttg ctccgatttc cggatgagat ttaatctttt caaattcttc tttttttaat
     1141 tttcccgttt tgttcagaat atttttgtct ataccgattt tacctatatc atgaagcaag
     1201 gctgcgcttt ttattaaatc aatcttgctc tgaggcagtc ccacctgtct tgctattgct
     1261 tccgcatatg cacttacacg catggagtgt ccgcttgtgt atgggtcgct tgcttctatg
     1321 gcacgaacaa gaacatttaa cgtttcaaaa taattcttac gcatgtccaa ataaagctta
     1381 aatgtgtatc ttgcgagtag aaggggtatg aaaaacaaga gcagtccgcc tgagccataa
     1441 ctataatatg caaatgccaa
//
LOCUS       DAOSOW010000426         1451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_88884_length_1451_cov_3.170487, whole genome shotgun sequence.
ACCESSION   DAOSOW010000426 DAOSOW010000000
VERSION     DAOSOW010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88884_length_1451_cov_3.170487"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /gene="dinB"
                     /locus_tag="PLD67_09110"
     CDS             complement(<1..796)
                     /gene="dinB"
                     /locus_tag="PLD67_09110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOT22723.1"
                     /translation="MKRVIFLVDMNAFYISCEKTRHPEIEGKPAAVAGDPVNRTGIIL
                     TANYEARKYGVKTTMILREAFRLCPDLIIIPPDHRFYEKKSQEVMQILSSYTPIVEQN
                     SIDEAWLDMTGCERLFGQPLESARSIMDRIKNELNLWCSIGISENKFLAKMASEMKKP
                     LGITELWKKDIEEKLWPLPVDYMYGVGKQTAKKLNSMGIYTVKDLALTKREDIYHKLG
                     KIGVELVLLANGKDDTPVNVNREDIKSIGRSTTLPNDITDIEDAKAI"
     gene            complement(833..>1451)
                     /locus_tag="PLD67_09115"
     CDS             complement(833..>1451)
                     /locus_tag="PLD67_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22724.1"
                     /translation="MLFITSIEFAGDFDKIPEFDDNVKLHFTMNMDMVTYLVEQPVTL
                     TVNGEYHEKLLFNNKEDNKEHWAQINRVYLLDMWAEMEKSFSDARLLEHMTKEQIEEA
                     KRNIEKSFVDVCPKGMYYPVIEYESEDDISLEFHTKKFLDSKPVHHGSGSIGFIISPD
                     KPTGILGKKLKSAIIQEPVTENTEIIEAELFQYHRTITPEDVILC"
BASE COUNT          396 a          333 c          215 g          507 t
ORIGIN      
        1 atattgcttt ggcatcttca atatctgtaa tatcattagg cagggtagtt gaccttccta
       61 tggatttaat gtcctccctg ttcacattta ctggagtatc atctttgccg ttagccagca
      121 aaaccaattc aacccctatt tttcctaatt tatgataaat atcttccctc tttgtcaagg
      181 ctaaatcctt aactgtataa atacccatgc tattaagctt tttagctgtc tgcttcccca
      241 ctccgtacat atagtcaacc ggtaaaggcc acaatttttc ttctatatcc tttttccaca
      301 gctcagtgat acctagcggc tttttcattt ccgaagccat ttttgccaaa aacttatttt
      361 cagatattcc gattgagcac cacaaattga gttcattctt tattctgtcc attatacttc
      421 ttgcagattc taagggctga ccaaacaaac gctcacaccc tgtcatatcc agccacgctt
      481 cgtcaatgct gttttgctca actatgggag tgtagcttga caaaatttgc ataacttctt
      541 gggacttctt ctcataaaat ctatggtcag gaggaattat aatcaaatcc gggcatagcc
      601 gaaaagcctc ccttaaaatc atggttgttt ttacaccata ttttcttgct tcataatttg
      661 cagtaagtat tattcccgta cggttgacgg ggtcccccgc aaccgctgct ggtttgcctt
      721 caatttcagg atggcgagtt ttttcgcagc tgatataaaa ggcattcatg tctaccaaaa
      781 atattaccct tttcatatat ataaccttca cattcattgg tgcctggcac cactaacaca
      841 aaattacatc ctccggagtt attgtcctgt gatactgaaa taattctgct tcaataatct
      901 cagtgttttc tgtaacaggc tcctgtataa ttgcagattt taatttcttt cctaagattc
      961 cggttggctt atcagggctg attataaatc caatgctgcc gcttccgtga tgaacaggct
     1021 ttgaatcaag aaattttttt gtatgaaatt caagcgagat atcgtcctca ctttcgtatt
     1081 cgataacagg ataatacatc cccttaggac aaacatcaac aaaacttttt tcaatattcc
     1141 tttttgcctc ttctatctgc tccttagtca tatgctctaa tagtcttgca tctgagaagc
     1201 ttttttccat ttcagcccac atgtccaata aataaaccct gtttatttga gcccaatgct
     1261 ctttattgtc ttccttgtta ttaaacaata acttttcgtg atattctcca tttacggtaa
     1321 gtgtaacagg ttgttcaacc aaatatgtaa ccatgtccat attcatggta aaatgcagct
     1381 taacattatc gtcgaattcc ggtatcttat caaaatcacc cgcaaattca atactggtta
     1441 taaacaacat a
//
LOCUS       DAOSOW010000427         1441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_89749_length_1441_cov_3.914863, whole genome shotgun sequence.
ACCESSION   DAOSOW010000427 DAOSOW010000000
VERSION     DAOSOW010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89749_length_1441_cov_3.914863"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="PLD67_09120"
     CDS             <1..847
                     /locus_tag="PLD67_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT22725.1"
                     /translation="LHMLTDKAMFDRMKDLGVMNWLEELKAEGTIKNIGFSFHGSKSD
                     FELIVKAYPWDFCQIQYNYLDENNQAGVSGLKLAHSLGIPVIVMEPLRGGKLVNNLPN
                     EVIKIFISAHPEWTPAEWGLRWIWNHKEVNVVLSGMSLEEQVNENIKIASNASFDSLS
                     EDELKVFDEIKSIMNRKVKVPCTDCRYCMPCPHGVNIPGCFSSYNDKFLLDDKLYRWK
                     YAQALGAFSAEPAFASKCVECGECEPKCPQNIEIRKELKAVSKAMEGIFFKPIVGTAR
                     KILRI"
     gene            complement(871..>1441)
                     /locus_tag="PLD67_09125"
     CDS             complement(871..>1441)
                     /locus_tag="PLD67_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513891.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOT22726.1"
                     /translation="KEMEGILVAPGFGDRGIEGKITACRFARENNIPFLGICLGMQIA
                     VIEFARNVCGLEDANSGEFNLDSPHRVIDIMKEQEKITQRGGTMRLGAYPCKVIQNTK
                     MRSLYNTSEISERHRHRYEFNNKYREIMTKKGLVISGVSPDDTLVEAVELSDNRFFVG
                     VQFHPEFKSRPNKPHPLFLGLIKAALKQE"
BASE COUNT          433 a          256 c          310 g          442 t
ORIGIN      
        1 gctgcatatg ctgacggata aagcaatgtt tgacagaatg aaagaccttg gggtaatgaa
       61 ttggcttgaa gagctgaaag ctgagggaac aataaaaaac atcggttttt cttttcatgg
      121 aagcaagtcg gattttgaac taattgtaaa agcttatcca tgggatttct gtcagataca
      181 atacaattat ttggatgaga ataatcaagc cggtgtatca ggacttaaac ttgctcattc
      241 tttgggaatt cccgtgattg ttatggagcc cttgagggga ggaaaattag taaataattt
      301 acctaatgag gttataaaaa tttttataag tgcccatcct gaatggacac ctgctgaatg
      361 gggactgaga tggatttgga atcacaagga agttaacgtg gtcttatcgg gaatgagcct
      421 tgaagagcag gtaaatgaaa atattaaaat tgcttcaaat gcatcttttg attctctatc
      481 tgaagatgag cttaaagtgt ttgatgaaat taaatccatc atgaacagaa aagtaaaggt
      541 tccctgtacg gattgcagat actgcatgcc ctgtccccat ggtgtcaata taccgggttg
      601 tttttcaagc tataatgaca agtttttgtt ggatgataag ctgtacaggt ggaagtacgc
      661 acaagcactg ggtgcatttt cagcagagcc cgcatttgct tcaaaatgtg tggaatgcgg
      721 tgaatgtgag ccaaaatgcc cgcaaaatat agagataaga aaagaattaa aggctgtttc
      781 aaaggctatg gaaggaatct tctttaaacc tatagttgga acagccagga aaatacttag
      841 aatttaatta aaggcagctg ctcattcaga ttattcttgt ttaagagcag cttttataag
      901 ccctaaaaat aatgggtgag gtttatttgg tcggctttta aattcggggt ggaactgaac
      961 acctacaaag aatcgattat cggagagttc gactgcttct accaaagtat cgtcaggcga
     1021 aactccactg attacaagtc ctttcttggt cattatttca cggtatttgt tgttaaattc
     1081 gtatcggtgc ctatgccgtt ccgatatttc cgatgtgtta taaagtgacc tcatctttgt
     1141 gttttgaata actttgcaag gataagcgcc aagccgcatt gtgccgcctc tttgcgtaat
     1201 tttttcttgt tccttcataa tatcaattac acgatgaggt gaatccaggt taaactcacc
     1261 cgaatttgcg tcttcaagtc cgcatacatt tcgtgcaaat tcaattactg caatctgcat
     1321 tcctagacaa atacctagaa atggtatatt gttttcgcgt gcaaagcggc aagccgtaat
     1381 tttgccctca ataccacggt cgccaaaacc tggtgcaact aatattcctt ccatttcttt
     1441 c
//
LOCUS       DAOSOW010000428         1437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_90114_length_1437_cov_3.583213, whole genome shotgun sequence.
ACCESSION   DAOSOW010000428 DAOSOW010000000
VERSION     DAOSOW010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90114_length_1437_cov_3.583213"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="PLD67_09130"
     CDS             complement(<1..186)
                     /locus_tag="PLD67_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006504894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOT22727.1"
                     /translation="MKNIVIIGMPGSGKTTVGMMLSKKLNMEFFDMDDNIVSMENKSI
                     NEMFEISEEYFRNAETRC"
     gene            complement(190..450)
                     /locus_tag="PLD67_09135"
     CDS             complement(190..450)
                     /locus_tag="PLD67_09135"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HOT22728.1"
                     /translation="MELDKARTEINKIDKEIVRLLEKRFNIVNEIGNYKRKQGLSIYD
                     ETREKQVVESCISYLENKEYSKFIDDIYFQIMKCSKDIQQEG"
     gene            complement(420..>1437)
                     /gene="aroC"
                     /locus_tag="PLD67_09140"
     CDS             complement(420..>1437)
                     /gene="aroC"
                     /locus_tag="PLD67_09140"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOT22729.1"
                     /translation="GYAINLDYINQQMNRRLPGKSNLSTERKEEDEYKILSGFLEGKT
                     TGTPLSAIIYNKDKKYEDYGDRFKLRPGHSDYTGYIKYSSYNDYRGGGHFSGRLTAPL
                     LFAGAIAMQLLESREIFIGSRIKSIYNINDENIENIDAITIKNLKKMSFPIISLEKGA
                     LMKTAIIEQKEKGNSLGGIVETFIINIPAGYGEPFFDSVESKISHIVFSIPAIKGIEF
                     GAGFKITELTGKEANDEFKVENNKIITSTNNNGGILGGITNGMPIVFRTAIKPTPSIS
                     LSQNTVDISSMENTIINTKGRHDPCIVPRVVPVIEGACALALLDLILERDGEKWSLIK
                     QEQK"
BASE COUNT          372 a          284 c          209 g          572 t
ORIGIN      
        1 acatctggtt tctgcatttc tgaaatattc ttcgcttatt tcaaacattt cattaatgct
       61 tttattttcc atggatacta tattatcatc catatcgaaa aattccatgt ttaatttttt
      121 tgaaagcatc atgccaacag ttgtttttcc gctccccggc atgccgatta taactatatt
      181 cttcattttt tatccttctt gttgtatgtc cttgctgcat ttcattattt gaaagtaaat
      241 gtcatcaatg aacttggaat attccttatt ttccaaataa cttatgcaag attcaacaac
      301 ctgcttttct cttgtttcat catatatgga aagtccttgt tttcttttgt aattacctat
      361 ttcattcact atattaaatc ttttttccaa tagtctgact atttctttat ctatcttgtt
      421 tatttctgtt cttgctttat caagctccat ttttcaccat ccctttccaa aattaaatca
      481 agtaaggcaa gagcgcaagc tccttctata acgggaacaa ctcgaggtac aatacaggga
      541 tcgtgacggc ctttagtatt tatgatagta ttttccatag atgatatatc aaccgtattc
      601 tgactcaagg atattgaagg agtgggtttt attgcggttc taaatactat gggcattccg
      661 tttgttattc ctcccaatat acctccgtta ttattggtag aagtaattat tttattattt
      721 tctactttaa attcatcatt ggcttctttt cccgtaagct cagtaatttt aaaacctgca
      781 ccaaattcaa ttcctttaat tgcaggtatg gagaatacta tatgtgatat tttgctttca
      841 accgaatcga aaaacggctc cccgtaccct gcaggaatat ttatgataaa tgtttccact
      901 attcctccca aggagtttcc tttttctttt tgctcaatta tggctgtttt cataagggct
      961 cctttttcta atgaaatgat ggggaaactc attttcttta gattttttat tgtaattgca
     1021 tcaatatttt cgatattttc gtcatttata ttgtaaatgc tttttatgcg gctgcctata
     1081 aatatttctc tgctttccaa taattgcata gcaattgctc ctgcaaataa aagcggtgcg
     1141 gtaagccttc cggaaaaatg acctcctccc cggtagtcat tatatgatga atattttatg
     1201 taacccgtgt aatcgctgtg accggggcga agcttaaacc tatcgccgta atcctcatac
     1261 ttcttatctt tattataaat tattgcggac agcggggttc ctgtagtttt tccttctaag
     1321 aaaccgctga gtattttata ttcatcctct tcttttctct cggttgacaa attgcttttt
     1381 ccgggaagac gtctgttcat ctgttgattt atgtaatcca aatttatagc atatccc
//
LOCUS       DAOSOW010000429         1434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_90404_length_1434_cov_3.092096, whole genome shotgun sequence.
ACCESSION   DAOSOW010000429 DAOSOW010000000
VERSION     DAOSOW010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90404_length_1434_cov_3.092096"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..550)
                     /gene="rimM"
                     /locus_tag="PLD67_09145"
     CDS             complement(50..550)
                     /gene="rimM"
                     /locus_tag="PLD67_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227124.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOT22730.1"
                     /translation="MKDYIKVGKIINTHGIKGYIKCMPMTDDLERFEELEYVYTEKDN
                     IKRVIDDVWYQKGMVYLKLRNIDDMNTAESYKDTYISILEDQLRELPEDSYYLFDLNG
                     MDVYSSTGEYIGKISEIYQTGANDVYEVRNNKSSFLIPAIKDVVKSVNIQEKKMIIDV
                     IEGLLE"
     gene            complement(567..797)
                     /locus_tag="PLD67_09150"
     CDS             complement(567..797)
                     /locus_tag="PLD67_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOT22731.1"
                     /translation="MGELVEFIAKSLVDNPDMVEVNEVEGSQSLIIELKVAPDDMGKV
                     IGKQGRIAKAIRTVVKAAATKDNKRVIVEIIQ"
     gene            complement(809..1072)
                     /gene="rpsP"
                     /locus_tag="PLD67_09155"
     CDS             complement(809..1072)
                     /gene="rpsP"
                     /locus_tag="PLD67_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOT22732.1"
                     /translation="MSVKIRLKRMGSKKKPFYRIVVADSRSPRDGRFIEEIGYYNPVV
                     EPKVVKINDESAIKWLNNGAKPTDTVSMLFKNNGIYEKRDENK"
     gene            complement(1193..>1434)
                     /locus_tag="PLD67_09160"
     CDS             complement(1193..>1434)
                     /locus_tag="PLD67_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOT22733.1"
                     /translation="LVYTEAIIQSMTKKERMNPSIINGSRRKRIAAGSGTSVQRVNSL
                     LKQYEEMKKMMKKFQAMGTGKKKGFGGMGGFKLPF"
BASE COUNT          373 a          300 c          192 g          569 t
ORIGIN      
        1 tttttataaa cttcaattaa ctccggaaat aaagttacta tttcaaattt cattccaata
       61 acccttcaat aacatcaatt atcatttttt tttcctgaat attaactgat ttcacaacat
      121 cctttattgc aggtatcaaa aatgagcttt tgttatttct tacttcatat acatcgttgg
      181 ctccggtttg atagatttct gaaatttttc cgatatattc accggtacta gaatacacat
      241 ccattccatt caaatcaaat aaatagtagg aatcttcggg aagttctctc agctggtcct
      301 caagtatgga aatgtatgtg tccttatatg attctgcagt attcatatcg tcgatatttc
      361 ttagcttaag ataaaccata cctttttggt accatacatc gtcaattaca cgctttatgt
      421 tgtctttttc cgtgtagacg tattctaact cctcgaatct ttccaaatca tcggtcattg
      481 gcatgcattt tatatagcct ttgataccgt gtgtatttat tattttgcca actttaatat
      541 aatccttcat ttctacctca gaaaggttat tgaattattt ctactataac ccttttatta
      601 tcctttgttg cagctgcttt aacaacagtc cttattgctt ttgctattct gccttgtttt
      661 cctattactt tacccatatc atcaggagct acttttaatt caattatcag tgattgactg
      721 ccttcaactt cattgacttc aaccatatcg ggattgtcaa caagagattt tgctataaat
      781 tctactaatt cacccatgat atcacctctt atttgttttc atctcttttt tcgtaaatgc
      841 cgttattttt gaaaagcata cttactgtgt cggtcggttt agctccgtta ttcagccatt
      901 tgattgcact ttcatcgttt attttcacaa ccttcggctc aactacagga ttgtagtatc
      961 ctatttcttc aatgaaacga ccatctcttg gagagcgtga atctgctact actattctgt
     1021 aaaaaggttt ctttttggaa cccattcttt ttaatctgat ttttactgac attatgtcac
     1081 ctccttgatt tagtgtgtgg gacacacctc tttttatgga gctatgctcc tgtttgagga
     1141 ataccctgaa tatatatgaa aaaggaaaat cctatttcta acaataatat ggttaaaatg
     1201 gcagcttgaa tccgcccata ccgccaaagc ctttcttctt gcctgtaccc atcgcttgga
     1261 attttttcat catttttttc atttcttcat actgctttaa taatgaattg accctttgaa
     1321 cagatgttcc gcttcccgca gctattcttt ttcttctgct gccgttgatt atcgaaggat
     1381 tcattctttc cttcttagtc atagattgaa taattgcttc cgtatacact aatt
//
LOCUS       DAOSOW010000430         1422 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_91525_length_1422_cov_2.745428, whole genome shotgun sequence.
ACCESSION   DAOSOW010000430 DAOSOW010000000
VERSION     DAOSOW010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91525_length_1422_cov_2.745428"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1422)
                     /locus_tag="PLD67_09165"
     CDS             complement(<1..>1422)
                     /locus_tag="PLD67_09165"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HOT22734.1"
                     /translation="VMMSRLYKIDDDIREEIIEKYKTSLKSMKNILYNEWSIDYLALA
                     IELGIVSEQAVKDMFANRNIFNEAKREEVAVLLTMALGLNDEVKSLKTYVLPFNDRDE
                     ITASARPYIYLMYEKEIMQGDNEKNINPGDKITRAEISTLLDKAYDYIDENNIFPDLE
                     EYKPTTTVSGMITEVQINKTESYIYVKNDSEVESIVKINNDTKITINGRTRVLSDLKK
                     DMLVICRINEERLALEIEADSTKDVVRGTIYYVAYVEPASITIYDEDDDKLTFDIGKD
                     VEVLHDGKATELRRLRKNDEVTILLDDDEVIKISSISRIKHYDGVITSIDYNYPIKVT
                     IKTDEDVSKTFIFNKDVEVTRNDDDSSFDQVRVGDEVTITTEYDEMIAINTIAKEAET
                     SGVIREIIIAPQSKIKIADEDGEIKQYSVSNNVKITIGTKNVSIYDLRLGYNVYINTS
                     GDEIVTMEVSEMETAKSFTGKII"
BASE COUNT          386 a          294 c          187 g          555 t
ORIGIN      
        1 atataatctt acctgtgaag ctcttggcag tttccatttc agaaacttcc atcgtaacaa
       61 tttcatctcc cgaggtgttt atataaacat tatatccaag gcgcaaatca taaatcgata
      121 catttttggt tccgattgta atcttgacat tgttgcttac ggaatactgt tttatttctc
      181 catcttcgtc tgcaattttt attttgcttt gaggtgcaat tattatttcc ctgattactc
      241 cgcttgtttc agcttctttg gcaatagtgt taattgcaat catttcatca tattctgtag
      301 tgattgtaac ttcatcgccg actctaacct ggtcaaagct tgagtcatca tcatttcttg
      361 ttacttcaac atccttgtta aaaataaatg ttttagaaac atcttcatct gtttttattg
      421 taacctttat aggataatta taatcaatgg atgtaataac tccgtcataa tgtttgatac
      481 ggcttatgga actaatttta ataacctcat catcatccaa taaaatggtc acttcatcat
      541 tctttctaag tcttctcaat tccgttgctt tgccgtcatg taatacttca acatctttcc
      601 cgatatcaaa tgtaagttta tcatcatctt cgtcgtaaat agttattgat gcaggctcaa
      661 catatgccac ataataaatt gtacctctta caacgtcctt tgtgctgtca gcctctattt
      721 ctaaggcaag cctctcctcg ttaatcctgc agataacaag catgtccttt tttaaatcag
      781 ataacactct tgttctcccg tttatcgtaa ttttagtatc attatttatt ttaacgatac
      841 tttcaacttc gctgtcattt tttacgtata tgtaggattc tgtcttgtta atttgtacct
      901 cggtaatcat accgctgact gttgtggtag gtttatattc ttctaaatcg ggaaaaatat
      961 tgttttcgtc tatataatca tatgctttat ctaacagagt agaaatctct gcccttgtaa
     1021 ttttatctcc cgggttaata ttcttctcat tatccccttg cattatttcc ttttcgtaca
     1081 taaggtagat atatggtcgt gcagaagctg taatttcatc tctgtcatta aaaggcagga
     1141 catatgtctt caagcttttc acttcatcat tcaaacccag agccatcgtc aacagcactg
     1201 ccacttcttc cctttttgcc tcgttgaaaa tatttctatt tgcaaacata tctttaacag
     1261 cctgctcgct aactatacca agctcaattg caagagccaa ataatctatt gaccattcgt
     1321 tatataaaat atttttcatt gattttaaag aagtcttgta tttttcgatt atttcttctc
     1381 taatatcatc atcgatttta tataatctcg acatcataac ca
//
LOCUS       DAOSOW010000431         1418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_91902_length_1418_cov_4.402788, whole genome shotgun sequence.
ACCESSION   DAOSOW010000431 DAOSOW010000000
VERSION     DAOSOW010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_91902_length_1418_cov_4.402788"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLD67_09170"
     CDS             complement(<1..317)
                     /locus_tag="PLD67_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HOT22735.1"
                     /translation="MERYPMLEFNLKKLYNNTKIVTKLCEDNGIKVAGIIKGFGGIAE
                     GAEEMVKGGCYQIGSSRIEQLKALKDRGFDMPLLLVRIPMMCEIEDVVKYSDISLVSE
                     KETL"
     gene            complement(327..779)
                     /gene="tadA"
                     /locus_tag="PLD67_09175"
     CDS             complement(327..779)
                     /gene="tadA"
                     /locus_tag="PLD67_09175"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005584387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOT22736.1"
                     /translation="MDEVFMEEALKEAYKAYEINEVPIGAVIVRKGEIVGRGFNQKET
                     LKDATLHSEITAIKDACKNLGGWRLPGCTMYVTLEPCPMCAGAIVNSRIERLVIGARD
                     LKTGACGSVLDITNNKKLNHQPEVKFGVLEKECTGILQDFFIKLRKKI"
     gene            1126..>1418
                     /locus_tag="PLD67_09180"
     CDS             1126..>1418
                     /locus_tag="PLD67_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22737.1"
                     /translation="MKTIKKLWALGLCGILVINMNSMAYADVLDDIIGSSTIEDTEDA
                     ENTEDVYTDVDNEDVTGNDITVTDENIAKYSVLFSDIDNHWAKEWIKNAVGLG"
BASE COUNT          412 a          277 c          245 g          484 t
ORIGIN      
        1 agagtttcct tttcagaaac caaggatatg tcactgtatt taacgacatc ctcaatttca
       61 cacatcatgg ggattctgac caataacaaa ggcatatcaa atcccctgtc cttcaaggct
      121 ttaagctgct caattcggga gctgcctatt tgataacagc ctccttttac catttcttcg
      181 gcaccttccg caattccgcc gaatccttta attatgcctg caaccttgat tccgttatct
      241 tcacatagtt ttgtaacaat tttggtattg ttgtaaagtt tttttaaatt gaattcaagc
      301 atagggtatc tttccatagg agcctcctat attttttttc taagctttat aaaaaagtct
      361 tgtaaaatac cggtgcattc tttctccaat acgccaaact taacctccgg ctggtgattt
      421 agctttttat tgtttgttat atccaaaaca gagccgcatg cacccgtttt taaatccctg
      481 gctcctatta ccagcctttc gattcttgaa ttgacgattg cgccggcaca catggggcaa
      541 ggctccaagg ttacatacat ggtacatccg ggaagacgcc atccgcccaa gttcttacag
      601 gcatccttta tggctgttat ttcagagtgg agagtggcat cctttaatgt ttctttttga
      661 ttgaaacctc tgccgactat ttctcccttt cttacaatta cagcaccaat gggaacctca
      721 tttatttcat atgccttgta tgcttctttc aaggcttcct ccataaatac ttcatccata
      781 actcttcctt tgttgcattt tattttaatt atagtttttt cattaattca agtcaacaat
      841 attgtctaag agcctgtctt aacataagca gaaattgcat gttatttaat ataccttttc
      901 aagaaggctt gtctccttat attacctgtt tactttcttc acaataaaat atagaattaa
      961 ttatatagtt ttaaacagcg aaagattccc tattagaggt atggggacac accttcaatg
     1021 aagggatagc ctttctgggt gagcattgaa ggaatgtccc cgtaaatatc catacttgga
     1081 acaaatgtta atttgattaa gtctaatata tgaggaggag gtactatgaa aacaataaaa
     1141 aaattatggg ctcttggtct ttgtggcata ttggtaatca atatgaattc aatggcttac
     1201 gctgatgttc ttgatgatat tataggcagc agtactattg aagatacgga agatgctgaa
     1261 aacactgaag atgtatacac agatgttgat aatgaagacg taactggaaa tgatatcaca
     1321 gtcactgatg aaaatatagc aaaatattcg gttttgtttt ccgacattga taatcattgg
     1381 gcaaaagaat ggattaaaaa tgcagttggt ttaggctt
//
LOCUS       DAOSOW010000432         1415 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_92207_length_1415_cov_3.789706, whole genome shotgun sequence.
ACCESSION   DAOSOW010000432 DAOSOW010000000
VERSION     DAOSOW010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92207_length_1415_cov_3.789706"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLD67_09185"
     CDS             complement(<1..288)
                     /locus_tag="PLD67_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HOT22738.1"
                     /translation="MFEEIIVGIICAIIGIIIGFFIRKNISESKIGSAELEAKRIIED
                     ANQKAETRKKEILVEAKDEAHRLRNEVERENKERRNEILRAEKRLIKKEEML"
     gene            complement(599..>1415)
                     /gene="recA"
                     /locus_tag="PLD67_09190"
     CDS             complement(599..>1415)
                     /gene="recA"
                     /locus_tag="PLD67_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010292709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOT22739.1"
                     /translation="VALHIVAEAQKKGGIAAFIDAEHALDPAYAKALGVTVEDLIISQ
                     PDTGEQALEIAEALVRSGAIDVIVVDSVAALVPKAEIDGEMGDSHMGLQARLMSQALR
                     KLAGAISKSNAVAIFINQLREKIGVMFGNPETTTGGRALKFYASMRLDVRRVETLKKG
                     DDVYGNRTRVKVVKNKVAPPFKQAEFDIIYGKGISKEGCILDMAVEAGVVNKAGAWYS
                     YENTRIGQGRENSKDYLLSNPELMDEIEKKVLEKYELGDLKVASDANDPLSYE"
BASE COUNT          373 a          288 c          201 g          553 t
ORIGIN      
        1 cagcatttct tcttttttaa tcagcctttt ttcagccctt aaaatttcgt ttcgtctttc
       61 tttgttttct ctttcaactt catttcttaa cctgtgagct tcgtcttttg cttcaacaag
      121 tatttctttt tttcttgttt cggctttttg atttgcgtct tcgattattc tttttgcttc
      181 taattcagca ctacctattt tgctttcaga tatattttta cgaataaaaa atcctataat
      241 tatacctatt attgcgcaga taataccgac tattatttct tcgaacaatt cagcacctcc
      301 aatattaaat tttcaagctg cgaaatatat acatttttcc taattgctat aaaaaatgtg
      361 tatcttcaaa aacatatatt ttaatcgtct taattagttt tgtaacctga atataattta
      421 ttttataatt atttataaac aatgtcaaga tataatgttt atatttgcat atttttacat
      481 attctaatgc atgtcaaagc gtgttggttt acataattta aattatgtaa actatggtat
      541 atagtttgtg gtatataata aaaaggcact tatagtgcct aagcgaaagt tctcaaagtt
      601 attcatatga taatgggtca ttggcatcag atgctacttt taaatctccc agttcgtact
      661 tttcaagtac ctttttctct atttcatcca taagctccgg atttgacaag aggtagtctt
      721 ttgaattttc tctgccttgt ccgattctcg tattttcgta ggaataccat gctccggctt
      781 tatttacaac tccggcttca acagccatat caagaatgca tccttctttt gatatacctt
      841 tgccgtatat aatatcaaat tcagcttgct taaaaggcgg tgcaactttg tttttaacaa
      901 ctttaactct ggttctgttg ccgtaaacat catcaccctt tttcaaggtt tctactcttc
      961 ttacgtctaa tctcattgaa gcataaaatt ttaaagcacg tccgccggtt gtggtttcag
     1021 gattgccaaa cattactcca attttttcac gcagctggtt gataaatata gccactgcat
     1081 ttgatttgct tatagctcct gctaattttc ttaaagcctg cgacattaat cttgcctgca
     1141 gtcccatgtg agaatctccc atttctccgt ctatttcagc ttttggaact aaggctgcta
     1201 cagagtctac aactatgaca tctatggcac cgcttctgac taaggcttca gctatttcaa
     1261 gggcttgctc accggtatca ggctgtgata taatcaaatc ttcaaccgta acgccgagag
     1321 cttttgcata agcagggtca agtgcatgct ctgcatcaat aaatgcagct attcctccct
     1381 ttttttgtgc ttctgctact atatgtaatg ctact
//
LOCUS       DAOSOW010000433         1404 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_93275_length_1404_cov_2.720534, whole genome shotgun sequence.
ACCESSION   DAOSOW010000433 DAOSOW010000000
VERSION     DAOSOW010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93275_length_1404_cov_2.720534"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1404
                     /locus_tag="PLD67_09195"
     CDS             <1..>1404
                     /locus_tag="PLD67_09195"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOT22740.1"
                     /translation="LGWAAITHPDDLQENLDKYNQLQEGKIDSYSMQKRFIRPDGSIV
                     WVDMTVAKLNFEGSRYNHISIVQDITERKQMEDALIESERSKAVLLSNLPGMAYRCNY
                     DRDWTMQFVSEGCYELTGYKSGSLINNRDLSFNDLIAPEYQDILWNVWKKILIKKKPF
                     KYEYEIVSATGQRKWVLELGQGIYDEQGTVVALEGIIIDISERKEHENKLKFISEHYG
                     LTGLYNQRYFEELLVKESKLKRCDKKAIILINLKKINSVSLTYGYNFSRRLIVELAEK
                     LNMLNNNNIILFQISLDRFAFYIKKYKNEFELIEFCSKIFCKIKEVQILNNLGCRIGI
                     YEIEEDNWDLESIIKNVSVAADSVNNKSIFNYCFYNDELKAKVTRENDIRDELFKEIT
                     DDNSKTIYLQYQPILNIKTGKISGFEALARFKSQKLGLVQPSEFIPIAEEMQLIVPIG
                     LRVINEACKFIKKLETAG"
BASE COUNT          531 a          165 c          286 g          422 t
ORIGIN      
        1 gcttggttgg gcagcaataa cacatccgga tgacttgcag gaaaaccttg ataaatataa
       61 ccaattacaa gagggtaaga ttgacagtta ttcaatgcaa aaacggttta taaggccgga
      121 cggctcaatt gtatgggttg atatgactgt tgccaagctt aattttgaag gctcaagata
      181 taatcatata agtatagttc aggatattac cgaaagaaag caaatggaag atgctttgat
      241 agagagtgag cgaagcaagg cagtgctttt gtctaatttg cccggtatgg cttaccgctg
      301 caactatgat agggattgga caatgcagtt tgtttctgag ggttgttatg aattgacggg
      361 atataaatct ggcagcttaa tcaataatag ggacttgtct tttaatgatt tgattgctcc
      421 ggaataccaa gatatattgt ggaatgtgtg gaaaaaaatt cttatcaaga aaaaaccttt
      481 taagtatgaa tatgaaattg tatctgccac cggacaaaga aaatgggttt tggaattagg
      541 ccagggtatt tatgatgagc agggtaccgt tgtagcactt gaaggaatta ttattgatat
      601 ttcagaaaga aaagaacacg aaaacaaact gaaatttata agtgagcatt atggattaac
      661 gggtttatat aatcaaaggt attttgaaga gctcttggtt aaagaatcaa aattaaaaag
      721 atgcgataaa aaagcaatta tattaataaa tttaaaaaaa ataaactcgg tgagtttaac
      781 ttacggatat aatttcagca gaagacttat cgtagaatta gcagaaaaac tgaatatgct
      841 caataataat aatattatat tgtttcaaat ttccttggac agatttgctt tttatattaa
      901 aaaatataaa aacgaatttg aattaatcga attttgcagt aaaattttct gcaaaattaa
      961 ggaagtgcaa atattaaata atttaggctg cagaatagga atatatgaaa ttgaagagga
     1021 taattgggat ttggaaagta taataaaaaa tgtttctgtt gcggctgaca gtgttaataa
     1081 taaaagcata tttaattatt gtttttataa tgatgaatta aaggcaaaag taacgagaga
     1141 aaacgatata agggatgaat tatttaaaga aataactgat gataattcta aaaccattta
     1201 tcttcagtat caacctattt tgaatataaa aacagggaaa ataagcgggt ttgaagcttt
     1261 agcccgcttt aaaagccaaa aacttggtct tgtacaacct tccgaattca tacctatagc
     1321 tgaggagatg cagcttattg taccaatcgg attaagggtt ataaatgagg catgcaaatt
     1381 tataaaaaag ttggagacgg cagg
//
LOCUS       DAOSOW010000434         1403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_93330_length_1403_cov_4.115727, whole genome shotgun sequence.
ACCESSION   DAOSOW010000434 DAOSOW010000000
VERSION     DAOSOW010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1403
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93330_length_1403_cov_4.115727"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /gene="alaS"
                     /locus_tag="PLD67_09200"
     CDS             complement(<1..1262)
                     /gene="alaS"
                     /locus_tag="PLD67_09200"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOT22741.1"
                     /translation="MDRFSINNIRKEYLNFFKSKGHLTEQSFSLIPKNDKSLLLIGAG
                     MAPLKKYFTGAEIPPAKRMATCQKCVRTGDVENVGLTARHLTFFEMLGNFSFGDYFKN
                     EAIVWAWELLTDVLKIDKDRLWVTVYLEDDEAENIWHTKVGIPMEKIVRLGKDDNFWE
                     LEVGPSGPCSEIYYDTGEDRGCGSTDCKPGCDCNRFIEIWNLVFTQFDKDEEGVYHPL
                     PNPNIDTGMGLERVACVLQDKPTVFDVEPLVFIVNKVSEICKIPYGQNLESDVAMKVI
                     CDHSRAATFMIGDGVIPSNEGRGYVLRRLIRRAIRKGKSLGINGEFLTKTAKVVIDNW
                     KEVYPELGEKEKYILKVLSLEEERFKITIDQGLELLMKEIQKLKETNKKELDGNVAFK
                     LYDTYGFPSELTEEILKENGFSLNKEQF"
BASE COUNT          379 a          317 c          194 g          513 t
ORIGIN      
        1 ttaaactgct ctttatttag gctaaaacca ttttccttaa gtatttcttc tgtcaattct
       61 gaaggaaaac cataggtgtc ataaagtttg aatgctacat ttccatccag ctctttttta
      121 tttgtttctt ttagtttttg aatttctttc attaaaagtt caagaccttg gtcaatggtt
      181 attttaaatc tttcttcttc aagacttaaa acctttaata tgtatttttc tttttctcct
      241 aattccggat aaacttcttt ccaattatct attacaacct ttgcagtctt agttaaaaat
      301 tcgccattta tccctaatga tttgcccttg cggattgctc ttcttattaa tcgtcttaat
      361 acataacctc ttccttcatt tgaaggtatt actccatcgc ctatcatgaa ggtcgctgct
      421 cttgagtggt cgcagattac tttcattgca acgtcgcttt ctagattttg accataaggt
      481 attttgcaga tttccgaaac tttattgact ataaaaacta ggggctccac atcaaacacc
      541 gtgggtttgt cttgaagcac gcaagctacc ctttcaagtc ccattcctgt atctatattc
      601 ggattaggca ggggatgata tacaccttct tcgtccttgt caaattgtgt aaatacaaga
      661 ttccagattt ctatgaacct gttgcagtca catcccggtt tacagtcagt tgaaccgcag
      721 cctctatctt ctcctgtgtc ataatatatt tcagagcagg gtccgctcgg tcctacttct
      781 aactcccaaa aattatcatc ctttccaagc ctcactattt tttccatagg tattcctacc
      841 ttggtatgcc aaatattctc agcttcatca tcctccaaat atactgtaac ccaaagtcta
      901 tccttatcaa tcttcaaaac atcggttaat agctcccacg cccatacaat tgcttcattc
      961 ttgaaataat caccgaagga aaagtttccg agcatttcaa aaaaagtcaa atgtctcgct
     1021 gttaaaccta cattttcaac atcacccgtc ctcacacact tttgacaggt cgccattctc
     1081 ttagcaggcg gaatttcagc tcccgtaaaa tattttttta acggagccat acctgctcct
     1141 atcaataata agctcttatc atttttaggt atcaaagaaa aactttgctc ggttaagtgt
     1201 cccttgctct taaaaaagtt caagtattct ttacgaatat tattaattga aaatctatca
     1261 attttatcca ctttatttcc tccgtttcat agtataagca atatgtactt tattatgaaa
     1321 ttataatgta ttctttccat cattgtcaag aaacatttgg ggacggttct ttttgttccc
     1381 ggcgccatta tgccgtctga gtg
//
LOCUS       DAOSOW010000435         1397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_93948_length_1397_cov_3.535022, whole genome shotgun sequence.
ACCESSION   DAOSOW010000435 DAOSOW010000000
VERSION     DAOSOW010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_93948_length_1397_cov_3.535022"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="PLD67_09205"
     CDS             complement(<1..1227)
                     /locus_tag="PLD67_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOT22742.1"
                     /translation="MSRIYTIKAEYHDVENLTKILSEHNEIQFVSFMGVDIGGNGTDE
                     KIPVKLFIKDMEEMLKYGIQTDGSSVELQGIADLSDARVDLLPDTDCTWFVDYNYEYC
                     NDENGLPVGTLIIPSFIVHKNKLVCSRSTLRRAEVHFKNEIKNVLKNHPHIAESLGFK
                     YEDIDEVLLTAATELEMWVKTPEQSADFEELHASQVLKEQYWKRTMGTVRTVLEKIMR
                     LLECYDLKPEMAHKEVGGITNKIDAKGRITHVMEQLEIDWRFSSPIQASDNEIFVKQL
                     IEDQFRYHGLEVLFKAKPIEGVAGNGEHTHLGAAVRLKNNEKVNLFAPNNMQEEYLSE
                     IGYGALMGILKNYEVINPIVSATNDAFNRLKPGFEAPVCTVTSLGGTKDSPSRNRSVL
                     IGVIRSIDNKNTVRFEL"
BASE COUNT          379 a          310 c          209 g          499 t
ORIGIN      
        1 taattcaaat cttacggtat ttttattgtc tatacttctg attacaccga taagaactga
       61 ccggtttctc gaaggtgaat cctttgtacc tcctaaggaa gttacagtgc acacaggtgc
      121 ttcaaatccg ggtttaagcc tgttaaaggc atcatttgta gcagacacta tcggatttat
      181 aacttcgtaa tttttcagta tgcccataag agctccgtag cctatttcac ttaaatattc
      241 ctcttgcatg ttattaggag caaataaatt tactttttca ttattcttaa gcctcactgc
      301 tgcacctaag tgcgtatgct ctccgttacc tgcaacgcct tctattggtt tggctttgaa
      361 taatacttcc aatccgtgat atctaaattg gtcttcaatc aattgtttta caaaaatttc
      421 attatctgaa gcctgtatgg gacttgaaaa cctccaatca atttccagct gctccattac
      481 atgggttatt cttccctttg catcaatttt attggtaatg cctccaactt ccttatgtgc
      541 catctcaggc tttaagtcat agcattccaa caagcgcatt attttttcca ggactgttct
      601 gacagtaccc atagttcttt tccaatattg ctcttttaat acctgggatg catgaagttc
      661 ttcaaaatcg gcactttgtt ccggtgtttt cacccacatt tccaattcgg tggcagcagt
      721 tagcaaaacc tcatcaatgt cttcatattt aaaccctaag gattctgcaa tatggggatg
      781 atttttcaaa acatttttta tttcattttt gaaatgaact tcggctcttc taagtgttga
      841 cctggagcac actaatttgt ttttgtggac aataaaagaa ggaattatta aagttccgac
      901 aggaagacca ttttcatcat tacaatattc ataattatag tcaacaaacc atgtacagtc
      961 tgtatccggc agcaaatcaa cccttgcatc gcttaaatca gctataccct gcagctcaac
     1021 gctggagccg tctgtctgta tgccgtattt aagcatttct tccatatcct taataaacag
     1081 ttttacaggt attttttcgt ctgttccgtt tccgcctata tcgacaccca taaaggatac
     1141 aaattgtatt tcattatgct cgcttaaaat tttggtcaaa ttttcaacat cgtgatattc
     1201 agcttttatt gtataaattc tgctcatata ctctccttct agcccttgca tatatctata
     1261 agctcagagg gctcaaatat taaataatca ggttcatgtt ttctaaggct ttcaacggta
     1321 ttataacccc atcctaccga tgctatatcc attcctgcat ttcttgcagc agtaatatct
     1381 cttatttcat ctccaac
//
LOCUS       DAOSOW010000436         1376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_96141_length_1376_cov_2.654807, whole genome shotgun sequence.
ACCESSION   DAOSOW010000436 DAOSOW010000000
VERSION     DAOSOW010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96141_length_1376_cov_2.654807"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..577
                     /locus_tag="PLD67_09210"
     CDS             <1..577
                     /locus_tag="PLD67_09210"
                     /inference="COORDINATES: protein motif:HMM:NF037182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOT22743.1"
                     /translation="AKGFDFPNVTTVGVLSADAILNLPFYNASEKTFQLITQVSGRAG
                     RGDKRGNVYIQTYEPENFIINAAKNNDYELFLHEELKLRKEFAFPPFINIINICLISK
                     IEELVVKTANEKYLEVKETVKEMVKERSLLLYRPVPHNIYKVNDEYRINLYIKVSNHS
                     LSELKKILRTVYMEKDIENIKVSININTDTI"
     gene            590..1048
                     /gene="def"
                     /locus_tag="PLD67_09215"
     CDS             590..1048
                     /gene="def"
                     /locus_tag="PLD67_09215"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOT22744.1"
                     /translation="MALRNLRYEGDEILRKKSREVPLVDDRIRMLMEDMVETMYKYDG
                     VGLAAPQVGILKRVIVIDVEDGKVYKMANPNIINESGEQSAQEGCLSVPEKKGIVSRP
                     MKVIVEYTDENNETVKLEAEGLLARAICHEIDHLNGVLFIDRVTDDVIEE"
     gene            1057..>1376
                     /locus_tag="PLD67_09220"
     CDS             1057..>1376
                     /locus_tag="PLD67_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOT22745.1"
                     /translation="MNVIIMGTPGFAVPTLEKLHESRHNLVLVVTQPDKPSGRGKKLK
                     KSEVKVTAEKLGLKIFQPDKIKKQENIDLLKSYNPDVIVVVAYGQILNKEILTLPKYG
                     CINV"
BASE COUNT          547 a          182 c          292 g          355 t
ORIGIN      
        1 tgcgaagggg tttgacttcc cgaatgtaac tacggtagga gttctttcgg cagatgcaat
       61 actaaattta ccattttata atgcaagcga aaaaacattt cagcttataa cacaggtaag
      121 cggaagagcc ggcaggggag acaagagagg aaatgtgtac attcaaacat acgagcctga
      181 aaatttcata ataaatgcag caaaaaacaa tgactatgag ctatttttgc atgaagaatt
      241 aaaattgcga aaagaatttg catttccgcc atttattaat ataataaata tatgtttgat
      301 atcaaaaata gaggaattgg ttgtaaaaac agccaatgaa aaatacttgg aagtaaaaga
      361 aaccgttaaa gaaatggtaa aggaaagaag tctattgttg tatagaccgg ttcctcacaa
      421 tatatacaaa gttaatgatg agtaccgaat taatttatat ataaaggtgt caaaccatag
      481 cttgtcggaa ctcaaaaaaa tactaaggac tgtatatatg gagaaggata tagagaatat
      541 aaaggtaagc ataaatatca acacggatac aatttaggag ggttcaaaaa tggcattaag
      601 aaatttacga tatgaaggcg atgaaatatt aagaaaaaaa agcagggaag ttccccttgt
      661 tgatgataga ataagaatgc taatggaaga tatggtcgaa accatgtata aatatgatgg
      721 tgtcggactt gcagctccac aggtagggat attaaaacgt gtaatagtaa tagatgtgga
      781 agacggcaag gtatataaaa tggctaatcc taatattatt aacgaatcag gggagcaatc
      841 agctcaggaa ggatgcttaa gcgtccccga gaaaaagggc atagtttcta gacccatgaa
      901 agtgattgtt gagtatacag atgaaaataa tgaaacagtt aaactggaag cggaaggttt
      961 attggcaaga gcaatatgcc atgaaataga ccatttaaac ggagtattgt tcatagacag
     1021 agtaaccgat gatgtaattg aagaataggt gatgttatga atgtaataat tatgggaaca
     1081 ccgggttttg cagttccaac cttagaaaaa cttcatgaaa gcagacacaa tcttgttttg
     1141 gttgtaactc aaccggataa accgtcaggc agaggaaaaa aattaaaaaa atcagaagtg
     1201 aaagtaacgg ctgaaaagtt aggtcttaaa atttttcagc ctgataaaat aaaaaaacag
     1261 gaaaatattg atttattaaa atcatataat cctgatgtaa tagttgttgt tgcttacgga
     1321 caaatactaa acaaggaaat tttgacattg ccgaagtacg gctgcatcaa cgtcca
//
LOCUS       DAOSOW010000437         1375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_96270_length_1375_cov_2.567424, whole genome shotgun sequence.
ACCESSION   DAOSOW010000437 DAOSOW010000000
VERSION     DAOSOW010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96270_length_1375_cov_2.567424"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLD67_09225"
     CDS             complement(<1..793)
                     /locus_tag="PLD67_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22746.1"
                     /translation="MKKIMFLSVVILTLSFVTAGCSAIKEPLDISTSTNKEPPDISIF
                     IDNKEIEYVCAKNKWDGSVFDREDTFVTIFKEQKDIPIFINGSIAEISFKNNPPDAFQ
                     IMDILIDEESRQIYTDKEIINISVELINGKCSFKIEKHLASSLSSYYEPGKKDIRGFR
                     MIASWGENECEYAFIIKTYEIDRTEYLTGEIITDGDYEILSSGIGCICFVPDKESREI
                     IEDKYSSYDLYYLNDESYFLYYDDISVTEGLPDKLGIYKVRVKFDL"
     gene            complement(1004..>1375)
                     /locus_tag="PLD67_09230"
     CDS             complement(1004..>1375)
                     /locus_tag="PLD67_09230"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOT22747.1"
                     /translation="KLSDMEYSNLEIYGYVNNLDEMMDESVFIVTKPGGVTVFEAINK
                     EVPLIVLNSNIGQEIGNIEFIKKNKIGIVINDLDDLLYILDYNINNPDTSDKYVKNIK
                     RIKKTLNYSGLSIDSLFDGVI"
BASE COUNT          425 a          249 c          178 g          523 t
ORIGIN      
        1 ttaaatcaaa ttttactctt accttataaa tccccaattt atcaggtaag ccttctgtaa
       61 cagatatatc gtcataatat aaaaagtaac tttcatcatt taaatagtaa aggtcataag
      121 aactgtattt atcttcgata atttccctac tctccttatc aggaacaaag caaatacagc
      181 ctatgccgct tgatagtatt tcataatcac cgtcagttat tatttcacct gtcaaatact
      241 cggttctgtc tatttcatat gttttaatta taaaggcata ctcacactcg ttttctcccc
      301 aggaagcaat cattctaaat cctcttatat ccttttttcc cggctcataa tatgaactta
      361 aggatgatgc taaatgtttt tctattttaa aagagcattt tccattgatt aattcaacag
      421 aaatatttat tatttctttg tctgtataaa tttgtctgct ttcctcatca attaaaatat
      481 ccataatttg aaatgcgtcc ggcggattgt ttttaaatga aatttcagca atgctgccgt
      541 ttataaaaat aggaatatct ttttgttctt taaatatggt aacaaaagta tcctctctgt
      601 caaaaacact tccatcccat ttattttttg cacaaacata ttctatctct ttgttgtcga
      661 taaatattga aatgtcaggc ggttctttat tggttgaagt tgatatgtct aatggctcct
      721 ttatagctga acaccctgct gtaacaaatg acaatgttaa aatcacaact gataaaaaca
      781 tgattttttt cattatatcc ccccttacat taaaaccgag aacgctttct tcaagaacgt
      841 aatatggact cctgcttttg gtaaatagtg tatatatttt agacgtttat tatattggtt
      901 ttgttcatta agctgtgctg aaagttttaa ctttatttaa atttaaacca aaaggataca
      961 ccatcttctt tatttatatg ttaagcagta gggtatcttt cacttaaatt accccgtcga
     1021 acaaggaatc aattgaaaga ccgctgtaat tcaaagtctt tttgattctt tttatatttt
     1081 ttacgtattt atcggaagta tccgggttat tgatattata atccagtata tagagcagat
     1141 catcaaggtc gtttattaca ataccaattt tgtttttctt gataaattca atatttccga
     1201 tttcctggcc gatgtttgaa ttcagtacta tcaagggcac ttccttatta attgcttcaa
     1261 atactgttac gcctccgggc tttgtaacaa taaaaaccga ttcatccatc atttcatcca
     1321 aattattcac atatccgtaa atttcaaggt tagaatactc catgtcggat aattt
//
LOCUS       DAOSOW010000438         1370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_96768_length_1370_cov_2.936882, whole genome shotgun sequence.
ACCESSION   DAOSOW010000438 DAOSOW010000000
VERSION     DAOSOW010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96768_length_1370_cov_2.936882"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..1276
                     /locus_tag="PLD67_09235"
     CDS             35..1276
                     /locus_tag="PLD67_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOT22748.1"
                     /translation="MAFNMVAEHAIWPKVNDAIFGLAAKAKEAIDKYGKENVIDSTLG
                     ALVDDNGELICLNTVYKELKSLPNSAIAAYAQVAGQPDFLEVVQKVCFGKYKPNAYIR
                     AVATPGGTGSVRHGIYNYTNPGDSILVGDWHWAPYVTISEEYGRTVTNHELFNSKNEF
                     NIESFKEKFEELLNKQKRLVAVINTPANNPTGYSLSDEEWDEVLNIAKENAKDPENRI
                     IIMVDCAYIDYAGIGDERRKFFAKFSGLPKNILIIVAFSMSKGYTMYGMRSGAAIGIS
                     SDEDIAEEFYYSCMHSNRANWSNGTRAAMEIMTRIANDPEKTKLYEAEVNETKVMLRR
                     RADAFVEGAKEVGLEILPYRDGFFVSIPCKDAKKLSDELIKENVFAIPLKKGLRFAVC
                     AVSEEKCRIAPAIIKKALDRI"
BASE COUNT          495 a          208 c          288 g          379 t
ORIGIN      
        1 atgttagaat tgaagaaaaa aacgaggtga tgtaatggca tttaatatgg ttgcagaaca
       61 cgcaatttgg ccaaaagtca atgatgcaat ctttgggctt gcagctaaag caaaagaagc
      121 tatagacaaa tatggaaaag aaaacgtgat tgattcaacc cttggagcat tagttgacga
      181 taatggagag cttatttgct taaatacagt atataaagaa ttaaaatctt taccgaattc
      241 agcaattgca gcatatgctc aggtggcagg tcagcctgat tttttggaag tagttcaaaa
      301 agtttgcttc ggcaaataca aacctaatgc atatatcagg gctgtagcaa ctcccggggg
      361 aacaggctcc gtaagacatg gaatatataa ttatacaaat cccggagatt caattttggt
      421 aggagactgg cattgggctc catatgtaac tatttccgaa gaatacggca ggactgttac
      481 caatcatgaa ttattcaaca gcaagaatga atttaatatt gaatcattca aggaaaaatt
      541 tgaagaattg ctgaataaac aaaaaaggct cgttgcagtg ataaacacac ctgccaacaa
      601 tcctacggga tacagtcttt ccgatgaaga atgggatgaa gttttaaata ttgctaaaga
      661 aaatgcaaaa gaccctgaaa atagaattat aataatggtt gactgcgcat acatagatta
      721 tgcaggaatc ggagatgaga gaagaaaatt cttcgcaaaa ttttccgggc ttcctaaaaa
      781 tattttaatt attgttgcat tcagtatgtc aaagggatat acaatgtatg gcatgcgctc
      841 aggtgcagca atcggaatat cttctgatga agatatagct gaagaattct attattcatg
      901 catgcattca aacagggcaa actggtctaa cggtacaaga gctgcaatgg aaataatgac
      961 tcgtatagcc aatgaccctg aaaaaacaaa gttatatgaa gcggaagtca atgaaacaaa
     1021 ggtaatgctg agaaggcgag ccgatgcttt tgttgaagga gcaaaggaag tgggattgga
     1081 aattttgcct tacagagacg gattctttgt aagcatacct tgcaaggatg caaaaaaatt
     1141 gtccgatgag ttaattaaag aaaatgtatt tgctataccg ttaaaaaagg gacttcgttt
     1201 tgcagtatgt gccgtttccg aagaaaaatg caggatagca cctgcaataa tcaaaaaagc
     1261 tttggataga atttaatgtt attaaaatat tagggacaca atatacaata ttgcgtccct
     1321 tttatttgta gatgctcttt attaatctta cattattctt taaagctgta
//
LOCUS       DAOSOW010000439         1368 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_96985_length_1368_cov_2.613861, whole genome shotgun sequence.
ACCESSION   DAOSOW010000439 DAOSOW010000000
VERSION     DAOSOW010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96985_length_1368_cov_2.613861"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PLD67_09240"
     CDS             <1..226
                     /locus_tag="PLD67_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT22749.1"
                     /translation="YMAHDAFLLLKAAIEDAGVADPVKIAESLTKVTVQGVTGTIKLS
                     PEDHNPIGKEAAIVRVENADYIFQEKYGLQ"
     gene            267..1148
                     /locus_tag="PLD67_09245"
     CDS             267..1148
                     /locus_tag="PLD67_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOT22750.1"
                     /translation="MSYFLQQLINGLSLGSVYALMSVGYSLVYSIMNFSNFAHGGVIM
                     IGAYIGYFCLTLLGVPFFTAFILSAVGSGILAVSMERIVYRPLRKRNAPFLYFIISAM
                     GASIFLENIVIASPIGPTFRSYPAVFSTSPIMIGGIALGRIDLLMFIISTLSLIGLMY
                     IIEKTKIGLSIRATSYSIKASTLMGVNVDKVIFIIFLLGGLLAGLAGMLFGMKYIVYP
                     QIGNITTKSFIAAVFGGLGSLPGAIIGSILLGLIETMTSGYLSSEYRDLIAFVLLIGV
                     LIFKPTGLMGKTTEDKA"
     gene            1159..>1368
                     /locus_tag="PLD67_09250"
     CDS             1159..>1368
                     /locus_tag="PLD67_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22751.1"
                     /translation="MSWGYLSGILILTGINLMTVLGLSLLTGFTGLFSFGHAGFMAIG
                     AYTAAMFTLKFNILPESMFQLQFYLA"
BASE COUNT          404 a          216 c          285 g          463 t
ORIGIN      
        1 ttatatggct cacgatgcat tccttctgtt aaaagcagca atagaggatg ccggtgttgc
       61 tgacccggtt aaaattgccg aatccttgac aaaagtaact gtccagggcg taacaggaac
      121 aattaaattg agtcctgaag atcacaatcc gattggaaaa gaagctgcaa tagtaagagt
      181 tgaaaatgca gattatatat tccaagaaaa gtatggatta caataaaaat aggcgggtta
      241 aacccgccta tcattgagga ggtaatatga gctatttttt acagcaatta attaacggtt
      301 tgtccttagg tagcgtatat gccttaatgt ctgtaggata ctctcttgta tatagtatta
      361 tgaatttttc taactttgct catggcgggg ttattatgat tggagcatat attggatatt
      421 tctgtcttac gttgctgggg gtgccttttt ttactgcctt tatattatcc gcagtcggct
      481 caggaatatt ggctgtaagc atggaaagga ttgtttacag accacttaga aaaagaaatg
      541 caccatttct ttattttata atatctgcaa tgggtgcttc aatttttttg gagaacatag
      601 taattgcatc acctatagga cctacattca gatcttatcc cgctgttttt tctacttctc
      661 ccataatgat tggaggaatt gcattgggtc gaattgactt attaatgttc ataatatcta
      721 ccctaagctt aataggtctt atgtatataa tagaaaaaac caagattgga ttgtcaatca
      781 gggcaacatc ttacagtata aaagccagta ctttgatggg agttaatgtg gataaggtta
      841 tattcataat atttttgctt ggcggtttat tggcaggtct ggcgggcatg ctttttggaa
      901 tgaaatatat agtatatccc caaattggca atattacaac gaaatcattc atagctgctg
      961 ttttcggagg attaggcagt ttgccgggtg ccataatcgg ctccatactc cttggactta
     1021 tagaaacaat gacttccggt tatctatctt cagaatatag agatttaatt gcatttgtac
     1081 ttcttatagg cgtactaatt tttaagccta cgggattaat gggaaaaaca acggaagata
     1141 aggcttagga ggtagagtat gagctggggt tatttatcag gaattttgat attaacagga
     1201 ataaatttaa tgactgtatt aggtttatca ttgcttacgg gatttacggg tctattttct
     1261 tttggacatg ccggattcat ggcaatcggt gcatatacag ctgctatgtt tacattgaaa
     1321 tttaacattt taccggaatc catgttccaa ttgcaattct atttggca
//
LOCUS       DAOSOW010000440         1363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_97467_length_1363_cov_4.392966, whole genome shotgun sequence.
ACCESSION   DAOSOW010000440 DAOSOW010000000
VERSION     DAOSOW010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97467_length_1363_cov_4.392966"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLD67_09255"
     CDS             <1..368
                     /locus_tag="PLD67_09255"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOT22752.1"
                     /translation="IDEYGGTEGLITMEDIIEELVGEIWDEHDEVIEWFKKIGDDKYL
                     VSCNADIDDMFELLGVEIDEELDITTVNGFITMLFEGIPEEGGRIVYKDLDITVTKAE
                     AKRVLEIQVERIKKEESLH"
     gene            473..1048
                     /locus_tag="PLD67_09260"
     CDS             473..1048
                     /locus_tag="PLD67_09260"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOT22753.1"
                     /translation="MNKLWFGFVFCGLLFSFINKSPETVTNVLFFEIEKSVELILNLL
                     PIMAFWTGLMRIVEKTNILNKLSSFLKPLVRYLFKDVQNDANAVNAILMTLAANLFGI
                     GNSATAFGIKAMQEMQKSNLNKKTATKAMCMFLIINVSSIQLIPLNVIKLRADSGSVA
                     PSEIMVPTLLVTAFSTMVAIIFAKYYEGKEL"
     gene            1045..>1363
                     /locus_tag="PLD67_09265"
     CDS             1045..>1363
                     /locus_tag="PLD67_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HOT22754.1"
                     /translation="MNISDLFLPILIGLILFYGIIKGVDVYSAFVEGAIDGAKSALKI
                     FPYILAIIFAINLFIQSGAESFIVDKLTPITSFIGFPTELLSLSIIKPLSGGGSFGVF
                     QTIL"
BASE COUNT          453 a          179 c          273 g          458 t
ORIGIN      
        1 taatcgatga atacggcgga actgagggat tgattaccat ggaggatata atagaagagc
       61 ttgttggtga aatttgggat gaacatgacg aagtaattga atggttcaag aaaatcggag
      121 atgacaaata cttagtatcc tgcaatgctg atattgatga tatgtttgag ctgcttggcg
      181 ttgagattga tgaggagctt gatattacta cggttaacgg ttttataaca atgttgtttg
      241 agggtatacc ggaagaaggc gggaggattg tttataaaga tttagatatt acggttacaa
      301 aggcagaagc aaaaagggta cttgaaattc aggtagaaag gattaaaaag gaagaatctc
      361 tacattaaaa taaatatgaa ttaataataa agacagacat attaattaat atgtctgttt
      421 gatttttttg ttgcgcagta tcttttacca attacaaagc aggaaggata ttatgaataa
      481 actatggttt ggatttgttt tttgtggtct tttattttct tttattaata aaagtccgga
      541 aacagttaca aatgttctct tttttgaaat agaaaagtca gtcgaactaa tattaaattt
      601 attacccatc atggcatttt ggaccggttt aatgcgtata gtggaaaaaa caaatatatt
      661 aaacaaatta tcatcttttt taaagccctt ggtcagatat ttgttcaaag atgtacaaaa
      721 tgacgctaat gccgtaaatg caattttaat gacattggct gccaatctgt ttggaatagg
      781 gaactctgcc accgcttttg gtattaaagc gatgcaggaa atgcagaaga gcaatttaaa
      841 taaaaaaaca gctacaaagg ccatgtgcat gtttttgata atcaatgttt cttcgattca
      901 gcttatacca ttgaatgtta ttaaattaag agctgattca ggctccgtgg ctccttcgga
      961 aattatggtg cctactttgc ttgtaactgc attttcaacc atggttgcaa taatatttgc
     1021 caagtattat gaagggaaag aattatgaat atatcagatt tatttttgcc tatactgata
     1081 ggtttgatat tgttttacgg aataataaag ggtgtagatg tatattctgc atttgttgag
     1141 ggagcaatag acggcgcaaa gtcggctctg aaaatatttc cttatatatt agcaatcata
     1201 tttgcaataa atctatttat acaatcagga gctgaatctt ttattgtaga taaacttaca
     1261 cctataactt catttatagg atttccgact gaactattat cgctgtcaat tattaaaccc
     1321 ttgtccggag gaggttcatt cggtgtattt caaacaatac tag
//
LOCUS       DAOSOW010000441         1363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_97497_length_1363_cov_2.668960, whole genome shotgun sequence.
ACCESSION   DAOSOW010000441 DAOSOW010000000
VERSION     DAOSOW010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97497_length_1363_cov_2.668960"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            415..1251
                     /locus_tag="PLD67_09270"
     CDS             415..1251
                     /locus_tag="PLD67_09270"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOT22755.1"
                     /translation="MVRLCMTVNKLFKLPVHPFNLHNEGKKTYAQWQYEKGIDTIKFY
                     LKHTDVDEMFKDKVVLDIGCGAGGKTVFYASKGVKKIIGIEILEKYRDEAESLATEYG
                     LEDKFEFVQGDASNMPFEDEIFDTIIMNDAMEHVDKPEKVLEECYRVLKKDGKLYLNF
                     PPYNHPYGAHLSDAIGIPWVHVFFSEKTLINTYKELVKNLPDGAERINFRISKKEDGT
                     EYFSYINKMSIKRANNILQNSNFKLEYYSEEPLRNFLKYPAKMPVLKEFLVKMVVCVL
                     RK"
BASE COUNT          480 a          202 c          271 g          410 t
ORIGIN      
        1 ttttttgcag gttttccatt tcgtctaatt tataatcgct gattaatgct tttatgcggt
       61 catttaagta gcttagtaca actattgggt cattgctgtc acccggagcg gcataaatta
      121 ctgcagtgag tgctattgcc aaaattaaag taaatataat tatctttttc aatttaccaa
      181 ctcccttaaa agtatatatg acattttata ttaaataagt ataaatttca acataagaat
      241 gcaaatcttt tagagaatta cactttttcc ccgataggtg tccctgtatc ttgataggtg
      301 tgtcctcata tcttaataaa cataagttga actttacatt gaattaacca atgtgaaatg
      361 atataattgc aaaggagaaa acagtttgac aaatattagt aagaaggtta aagaatggtt
      421 agactatgta tgacagtaaa caaattattt aagctgccgg tacacccctt taatttacat
      481 aatgaaggca aaaagactta cgcccagtgg caatatgaaa aaggaataga tacaattaag
      541 ttttatctta agcacactga tgttgatgaa atgttcaagg ataaggttgt attggatatt
      601 ggatgcggag ccggaggaaa aacagttttt tatgcttcaa aaggagtaaa aaaaataata
      661 ggtattgaaa tattggaaaa gtatagagat gaagcggaaa gcttagcgac agaatacggt
      721 ttagaggata aatttgaatt tgtacaaggc gatgcttcaa acatgccttt tgaagatgaa
      781 atatttgata ctataataat gaatgatgcc atggagcatg tcgacaaacc tgaaaaggtg
      841 cttgaagaat gctaccgggt tctaaaaaag gacggaaagc tgtatttgaa tttccctccc
      901 tacaatcatc cttacggcgc tcacttaagc gatgcaatcg gtataccatg ggtgcatgtg
      961 tttttcagcg aaaaaacact tattaataca tataaggaat tagttaagaa tttgcccgac
     1021 ggagccgaaa gaattaactt tcgaataagc aaaaaagaag acggcacgga gtatttttca
     1081 tatatcaata aaatgtcaat aaaaagggca aacaatattt tacaaaacag taattttaaa
     1141 ttagaatatt attcggagga acctcttaga aatttcttga aatacccggc taagatgcct
     1201 gtattaaaag aatttttggt taagatggtt gtttgtgtgt tgagaaagtg aaccagcggg
     1261 gacggttctc ggtggaccag cggggacggt tctcggtgga cactagatga cactaccccc
     1321 gtccccttgt cataaattta ttgtaaggag ataagatgaa act
//
LOCUS       DAOSOW010000442         1342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_99724_length_1342_cov_5.222999, whole genome shotgun sequence.
ACCESSION   DAOSOW010000442 DAOSOW010000000
VERSION     DAOSOW010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99724_length_1342_cov_5.222999"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="PLD67_09275"
     CDS             <1..635
                     /locus_tag="PLD67_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HOT22756.1"
                     /translation="GIAAMIFQGASFASVLDAMQNGVVSETGNAVLDELLTKGGYQSM
                     MWTISLILAALTFGGVLEKTGMLETVAKRILKFAKSTGALVTATIFTSIFTNILAGDQ
                     YLAIVLPGKMYQDEYRRRGLAPRNLSRCLEDAGTLTSPLVPWNTCGAAMSTYLGVPTI
                     EYLPYCFLNLVNPIVSIVFGYTGITMMKLEDDPSAPEYKGPKSKNKGVSA"
     gene            complement(676..900)
                     /locus_tag="PLD67_09280"
                     /pseudo
     CDS             complement(676..900)
                     /locus_tag="PLD67_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003144572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
     gene            1144..>1342
                     /locus_tag="PLD67_09285"
     CDS             1144..>1342
                     /locus_tag="PLD67_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22757.1"
                     /translation="MNLGIDGRVITWHIGSGLATYTNNLLENLNKLKHLRNIFLFYPY
                     KDGTDYFEINDIPKNLLIGERR"
BASE COUNT          391 a          311 c          260 g          380 t
ORIGIN      
        1 gcggaattgc ggcaatgata ttccaaggcg cttcctttgc aagtgttctt gatgcaatgc
       61 agaacggtgt tgtttcagaa acaggaaatg cagtacttga tgaacttctt acaaaaggtg
      121 gctaccagag tatgatgtgg acaatttcac ttatattggc agctttaacc tttggcggtg
      181 ttttagagaa aaccggcatg ttagagactg ttgctaagag aattcttaag tttgctaaga
      241 gcacaggcgc attagtaact gctactatat ttacaagcat atttacaaat atacttgccg
      301 gagaccaata cctagcaata gttcttccgg gtaaaatgta ccaggatgag tatcgcagga
      361 gaggtcttgc accaagaaac ctttcaagat gtcttgaaga tgccggtaca cttacttcac
      421 cgctcgttcc gtggaatacc tgcggtgctg caatgtcaac ctatcttgga gtaccgacaa
      481 ttgagtacct tccatactgc ttcctgaact tggttaaccc gatagtatcc attgtattcg
      541 gttacacagg aatcactatg atgaaactgg aagatgaccc ctccgctccg gaatacaaag
      601 gcccaaaatc aaaaaacaaa ggcgtatcag cctagtaaat ggtaaaagcc tgttgcagca
      661 gcaacaggct ttttttcata tctcgatcaa ttccttcgtg aacttgttca gcacctcaac
      721 tccggtctcg gtaacaatta ccaggtcttc tatccttacc ccgaatttcc ccggaagata
      781 gatgcccggc tctatgctga acaccatacc gggctgcaga acagtactgc tgatatctga
      841 tatgtacggc tcctcatgaa cctccatacc tatcccatgt cctgtcctgt gtatgaaata
      901 gaggtgcttc ataagcttcg acagcctgca tatccttgaa tccgggtttc ctgtttcatc
      961 gtaattgttc attcgtttcc ccctaaaaat caacttaatt aatacaatta tatcacattc
     1021 ttttcccctg cacatattat gattaaaaag gcgtctcaca ccgcaccttt ccctgtatcc
     1081 ccattatata ggaaagcata ctttactata caataatatg cgtaattcgg gaggctaaaa
     1141 aacatgaacc ttggcattga cggaagagta ataacctggc atatcggctc cggcttggcc
     1201 acctatacca ataacctgct ggaaaacctg aacaagctta agcatttgag aaacatattt
     1261 ttgttttacc cctataagga tggaaccgat tattttgaaa tcaatgatat accaaaaaac
     1321 cttctgattg gtgaaaggcg cc
//
LOCUS       DAOSOW010000443         1318 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_102394_length_1318_cov_3.874109, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000443 DAOSOW010000000
VERSION     DAOSOW010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102394_length_1318_cov_3.874109"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLD67_09290"
     CDS             <1..210
                     /locus_tag="PLD67_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019688220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOT22758.1"
                     /translation="IENGKIKEPVRGATLIGKGMEVLNKIDMVGNNLLQSQGMCGSVS
                     GSIPANVGQPMIRVSELTVGGREGE"
     gene            213..>1318
                     /locus_tag="PLD67_09295"
     CDS             213..>1318
                     /locus_tag="PLD67_09295"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOT22759.1"
                     /translation="MEMKSLIDKIFQKGREKGLNDMEVYYSAGSSLTLKVFQKELETY
                     NLSESEGLSLRGVYKGKMGYSYTEKVDETSIDQLVRDVLENAVIIDSDDEEFIFEGSK
                     EYIKVDSFNPALSNVSEEKKIEFVKSLEEEAFKLDKRIESVEVCVYGDGYGEIIMSNT
                     KGLFLQDKSNIAYTYIVVVAKEGEDIKTGMAYRTGNDFNKFNARDIAKEAVDEALALL
                     GAKSVKSGDYNVIIRNSAAADLLEAFTGIFSAEAVQKNLSLLKGKLNEKIASDKFTLI
                     DDPYMEGGLASKSFDGEGVACKYKKVVDKGVLKTYFHNLKTAKKDGVETTGNASKGSY
                     KSSLGISPSNFYVEKGERGLNEMIADINKGILIT"
BASE COUNT          489 a          179 c          306 g          344 t
ORIGIN      
        1 attgaaaacg gtaaaataaa agagcctgta aggggagcta cgctgatagg aaaaggcatg
       61 gaagtactta ataaaattga tatggtagga aacaatctgc ttcaaagcca gggtatgtgc
      121 ggttccgtaa gcggaagcat accggcaaat gtaggccagc ctatgattag ggtttcggaa
      181 ttaaccgtag gcggaaggga aggtgagtaa taatggaaat gaaatcattg attgataaaa
      241 tatttcaaaa aggcagagaa aagggcctga atgacatgga agtttattat tctgcaggaa
      301 gtagccttac attgaaggtt tttcagaaag agcttgaaac gtataatttg tcggaaagcg
      361 aaggactttc cttaagaggg gtttacaagg gtaaaatggg atattcatat accgaaaagg
      421 tagatgaaac ttcaatagac caactggtta gagatgtttt ggaaaatgcc gtcataatag
      481 attcggacga tgaagagttt atatttgaag gctcgaagga gtatataaaa gtagattcat
      541 tcaatccggc tttgtcaaat gtaagtgaag aaaagaaaat tgaatttgtt aaaagtcttg
      601 aagaagaagc atttaaatta gacaaaagaa ttgaatctgt tgaagtatgc gtttacggcg
      661 acggttacgg agaaataata atgtccaata caaagggtct tttcctccaa gataaaagca
      721 atattgcata tacctacata gttgttgttg caaaagaagg tgaagacata aagacgggta
      781 tggcataccg tacagggaat gattttaata aatttaatgc ccgtgatatt gcaaaggaag
      841 ccgtagatga agcattggct cttcttgggg caaaatcggt aaaatcagga gattacaatg
      901 tgattatcag aaattccgct gcagctgatt tattggaagc atttactgga attttctcag
      961 ctgaagcagt gcaaaagaat ctttccttac taaaaggaaa attaaatgaa aaaatagcaa
     1021 gcgacaaatt cactttgata gacgaccctt atatggaagg cggtcttgca tccaaatcct
     1081 ttgacggaga aggggttgca tgcaaatata aaaaggttgt tgataaaggt gttttaaaaa
     1141 cttattttca taatttaaaa actgccaaaa aagacggcgt tgaaacaaca ggaaatgcaa
     1201 gcaagggctc ctataaatcg tcattaggca tatctccttc aaacttttat gtagaaaagg
     1261 gagaaagggg attaaatgaa atgattgcag acataaacaa aggaatactc atcactga
//
LOCUS       DAOSOW010000444         1310 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_103370_length_1310_cov_3.366534, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000444 DAOSOW010000000
VERSION     DAOSOW010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103370_length_1310_cov_3.366534"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1228)
                     /gene="ytvI"
                     /locus_tag="PLD67_09300"
     CDS             complement(155..1228)
                     /gene="ytvI"
                     /locus_tag="PLD67_09300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HOT22760.1"
                     /translation="MTESEKKKFILDIAFIVTVYAVLYFIFVDVIHWVMPFIVGFLIA
                     LALRPVSRFVNRFVRSTGKGVALFVITMFYVVLAIIIWFLTSFLITQISELIHIMPRL
                     YFNRIEPVLLEFNDWVVQNAETLSPNVASTISQIITNGINYLADFIKNISISFVQLAT
                     RLISNFPLYLISVIFTIVLSVFISLEYDNITAFFRRQMPEKFKDTFEEARKFITGTLW
                     KMIKSYILIMIITFIETFTGLSILKVNYALPIAAIIAVLDVMPVLGTGGVLIPWAIFE
                     FILKNYFLGFGLLALYIAVTVIRHIIEPRIVGHQIGLHPIITITAMYAGLRLFSVAGV
                     ILGPIVAITVKYLNDEGKIKLFK"
BASE COUNT          467 a          229 c          204 g          410 t
ORIGIN      
        1 ggacacacct ctagtacagg gcaagtctct gtagtaagat gaatgtcccc gaatagtacg
       61 ccgtaaaata taaaagcaag gttcagcctt gctttttatt cgttattgct atgaatatag
      121 cggagcgtca ttccgaacgc agtgaagaat ctcatcattt aaacaatttt attttccctt
      181 catcatttaa atatttaaca gttatggcaa caataggacc taatattact cctgccactg
      241 aaaatagcct taaacctgca tacatagcgg ttattgttat tataggatga agtcctatct
      301 ggtgacctac aattcttggc tctataatat gtcttataac ggtaactgca atgtataaag
      361 ctaataatcc aaacccaaga aaataatttt ttaaaataaa ttcaaaaatt gcccaaggta
      421 tgagtacacc acctgttcct aaaaccggca taacatcaag aaccgctata attgcagcaa
      481 ttggcagggc ataattaacc tttaatatag acaagccggt aaatgtttca ataaatgtaa
      541 ttatcattat tagtatgtag gattttatca ttttccacaa tgtaccggtt atgaattttc
      601 gtgcttcttc aaatgtatct ttaaattttt caggcatctg tcttctgaaa aaagctgtaa
      661 tattatcata ttccaagctg ataaataccg aaagtacaat agtaaatatt acagatatta
      721 gatacagcgg gaaatttgat attaatcttg tagcaagttg tacaaaagaa atagaaatat
      781 tttttataaa atctgcaaga taattgatac cgtttgttat gatttgtgaa atagtacttg
      841 caacatttgg tgaaagtgtt tctgcatttt gaacaaccca atcgttaaac tcaagaagca
      901 ccggttcaat tctgttaaaa tataatctgg gcattatatg gataagttct gaaatttggg
      961 tgattaaaaa ggatgtaagg aaccatatga ttattgctaa aacaacgtaa aacattgtta
     1021 ttacaaatag agcaacgcct ttcccggtac tcctgacaaa cctgtttaca aatcttgaaa
     1081 caggcctgag agccaaagct attaaaaagc ctactataaa aggcataacc caatgaatta
     1141 catcaacaaa gataaaataa agaaccgcat atactgtaac aataaaagca atatctaata
     1201 taaatttttt cttttcgctt tcagtcatca acaagacctc gatttttttt aattgggaac
     1261 attcctaaag gcgtgtccta tactatacca tgaaggcgtg tccctcatga
//
LOCUS       DAOSOW010000445         1309 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_103511_length_1309_cov_2.830144, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000445 DAOSOW010000000
VERSION     DAOSOW010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103511_length_1309_cov_2.830144"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..175
                     /locus_tag="PLD67_09305"
     CDS             <1..175
                     /locus_tag="PLD67_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017728599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOT22761.1"
                     /translation="TKDLAKGGIHAGNMVKEAAVIAGGGGGGRPDFAQAGGKNPEKID
                     EAINAVRKQIASI"
     gene            313..576
                     /locus_tag="PLD67_09310"
     CDS             313..576
                     /locus_tag="PLD67_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HOT22762.1"
                     /translation="MDSNVNNTAKFDYSMGSLNEAREIISQVYKSLKEKGYNPNNQLI
                     GYILSGDPTYITNHNNARSLIRKIERDELLEEILSVYLNVIDI"
     gene            687..>1309
                     /locus_tag="PLD67_09315"
     CDS             687..>1309
                     /locus_tag="PLD67_09315"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOT22763.1"
                     /translation="MKYINLFEKKNVSRLCYGTLSLSSLQNLDTVENKVRLLNYAYEK
                     GINFFDTAQLYDNYNILKEFIKHKDRDKLVIASKSYAYDKNTAEYSINKALKEMNTDY
                     LDIFMLHEQDNGNNFLGHYKAVERFMKYKEQGIIKYFGISTHRVEAVKDSLKFKEIEI
                     IFPLINYKGIGIQDGTFEDMTEALKEAKENNKFIMAMKIYGGGHLIK"
BASE COUNT          503 a          165 c          270 g          371 t
ORIGIN      
        1 aacaaaggat ttggcaaaag gcggaataca tgcaggaaat atggttaaag aggcagctgt
       61 gattgcaggc ggaggcggag gcggaagacc tgactttgcg caagcaggag ggaaaaaccc
      121 cgagaaaata gacgaagcaa ttaatgcggt aagaaaacaa atagcctcta tatgacaagg
      181 ggacgggggt actgtcatca ttttgagtac cattagcacg aagaatttca tgttgtttta
      241 tatcataata tagtaattga ttttatttaa aaactatagt agaatataag taggtgtaaa
      301 gaggtggttt ttatggatag taatgtaaat aatacggcaa aatttgatta cagtatgggt
      361 tcattgaatg aagctcggga aataatttca caggtctaca agtcgcttaa agaaaaggga
      421 tacaatccta acaaccagct tatcggatat atattatcgg gggaccctac ttatattaca
      481 aatcataata atgcaagaag tttaatacga aaaatagaaa gagacgaact gttggaagaa
      541 attttaagtg tttacttaaa tgtaatcgac atttagagga ggggggacac attttgaatg
      601 tccctgtact aacttatatc ttgagcagta tataaatata ctgcttttgt atgccctaac
      661 aagtttcttc cgataaaaat ataaatatga aatacataaa tttatttgaa aagaaaaatg
      721 tatcaaggct gtgttacgga acactatctt taagcagtct tcaaaatctc gatactgttg
      781 aaaataaagt aaggctgctt aattatgcat acgaaaaagg aataaatttt tttgacacag
      841 cccagcttta tgataactat aatatactta aagaattcat aaaacataaa gacagggata
      901 aactagttat agcttcaaaa agttatgcat atgacaaaaa tactgcggaa tatagtatca
      961 ataaagcatt gaaagaaatg aacactgatt atttggatat tttcatgctt catgaacagg
     1021 ataacgggaa taattttttg ggtcattata aggcagttga gcgtttcatg aaatataagg
     1081 agcagggaat aatcaagtac tttggaattt caactcacag ggtggaggca gtaaaagatt
     1141 cacttaaatt taaggaaata gaaattattt ttcctttgat caattataaa ggtatcggaa
     1201 ttcaagacgg aacttttgaa gatatgactg aagccttgaa ggaggcaaag gaaaataata
     1261 agtttattat ggcaatgaaa atatatggcg gaggccattt aataaagga
//
LOCUS       DAOSOW010000446         1303 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_104216_length_1303_cov_2.915865, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000446 DAOSOW010000000
VERSION     DAOSOW010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104216_length_1303_cov_2.915865"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>252
                     /locus_tag="PLD67_09320"
     CDS             <1..>252
                     /locus_tag="PLD67_09320"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22764.1"
                     /translation="KVMSYFNLFLEKTFKGKKSNDVELIENIDTQSYEKDIVRTIASE
                     DTKKDLEIFNESRYEPESDSEVYLTSKRKENFFKVISKT"
     assembly_gap    253..262
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <263..938
                     /locus_tag="PLD67_09325"
     CDS             <263..938
                     /locus_tag="PLD67_09325"
                     /inference="COORDINATES: protein motif:HMM:NF019097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HOT22765.1"
                     /translation="SRSSAPRRKLFINAASVINNFNIVLFHTHGTEAFEESYETNYRS
                     LDENLNILGIGAKISNNLLNKGIKNTHLKDYNDYPDYNSSYANSNYAVRQVLSDSKKN
                     ILIDLHRDGAEENSNYEAILSEVKTTEINGKKAATCTLVVGNKNSNASKLKENAGRLY
                     EIAYEMYPGLMRKIIFREGAYFNQYLSDYSFLIEVGCTLNTYEEIEYTADLLTEVLYE
                     YINEIE"
     gene            1006..>1303
                     /locus_tag="PLD67_09330"
     CDS             1006..>1303
                     /locus_tag="PLD67_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOT22766.1"
                     /translation="MDRKKYIRNFSIIAHIDHGKSTLADRLIENTGLLTHRQMQEQVL
                     DNMDLERERGITIKLQTISLLYKAKDGNNYTLNLIDTPGHVDFNYEVSRSLAACE"
BASE COUNT          497 a          178 c          244 g          374 t
ORIGIN      
        1 ataaggttat gtcttatttc aacttatttc ttgaaaaaac ttttaaaggg aaaaagagta
       61 atgatgtaga gttaatcgag aacattgata cacaatctta tgaaaaagat attgttagaa
      121 caatagccag cgaggatacg aagaaagatt tggaaatttt taatgaaagc cggtatgaac
      181 ctgaatctga cagtgaagta tacctgacaa gtaaacgaaa agagaatttt tttaaagtta
      241 tttcaaaaac atnnnnnnnn nnatcaagaa gcagcgctcc aagaagaaaa ttattcataa
      301 acgctgcttc tgttattaat aattttaata ttgtgttgtt tcacactcat ggaaccgaag
      361 catttgaaga aagttatgaa acgaattacc gctccttgga tgaaaattta aatattttgg
      421 ggataggagc aaagatttca aataatttgc ttaataaagg cattaagaat actcacttaa
      481 aagattacaa tgattatccg gattacaact catcatatgc aaattcaaat tatgcggtaa
      541 gacaagtttt gtcagacagc aaaaaaaata tactaattga tttacaccgt gacggtgccg
      601 aagagaattc taattatgaa gcaattcttt cggaagtgaa aactaccgag ataaacggta
      661 aaaaagctgc aacctgtacc ctggttgtgg ggaataagaa ttcaaacgct tcgaaattaa
      721 aagaaaatgc aggaagactg tatgaaatag cttatgaaat gtatccggga cttatgcgaa
      781 aaatcatttt tagagagggt gcatatttta atcagtattt atcagattat tctttcctca
      841 tagaggtggg atgcacatta aatacatatg aagaaattga atataccgcg gatttattaa
      901 ctgaagtatt atatgagtat attaatgaaa ttgaataata atcttttaca attacgtcaa
      961 catttgatat aatacccata gcttaaattt gggagggtaa cttatttgga cagaaaaaaa
     1021 tatataagga acttttcgat aatcgcccat atagaccatg ggaaatctac attagcagac
     1081 aggttgatag aaaatacggg gcttcttaca cacaggcaaa tgcaggagca ggttttggac
     1141 aatatggacc tggaaagaga aagaggaatt acaattaagc tccaaaccat aagtttattg
     1201 tacaaggcta aggacggaaa taattacact cttaatttaa tagatactcc gggacatgtc
     1261 gattttaact acgaagtttc aaggagctta gcagcttgtg aag
//
LOCUS       DAOSOW010000447         1294 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_105282_length_1294_cov_4.055690, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000447 DAOSOW010000000
VERSION     DAOSOW010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105282_length_1294_cov_4.055690"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="PLD67_09335"
     CDS             complement(<1..662)
                     /locus_tag="PLD67_09335"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOT22767.1"
                     /translation="MDKQTIARLAAEFVQKEINYVSNDKALNEELAGLKIFDEPIFAY
                     GAADDEGFQLLKSPSVIGEHFMMPKEWLQSANTVIVYFFPFTDEIREGNRTDMSWPSN
                     EWLHGRIDGQIFMNEFNLFLNSKLNEEGYKSLVPGLDSRFKSEGFSSNWSERHVAFIC
                     GLGTFGLSKGIITQKGMAGRLGSIVTELKLKSDIKKYNNIYEYCINCGICAENCPAGA
                     IS"
     gene            872..>1294
                     /locus_tag="PLD67_09340"
     CDS             872..>1294
                     /locus_tag="PLD67_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOT22768.1"
                     /translation="MITYNKHIEYLHELKRKIMLHRHRLTIIMMLVILLGTMASGCAI
                     GETDVTNPDTTTGESIVVTITDPPDELEPIVVPTMPEVIPPYLGADPETGLTMTGTPT
                     LVDFDTYRLKVSGKVDQELSLAYDDLRRMPKLTATPTLE"
BASE COUNT          382 a          283 c          215 g          414 t
ORIGIN      
        1 aaggatattg cacctgcagg acaattttct gcacatatac cgcaatttat gcagtattca
       61 tatatattat tatatttttt tatatccgat tttaatttta attccgtcac tatacttcct
      121 aatcttccgg ccattccttt ttgagtaatt atcccttttg ataatccaaa agtcccaagc
      181 ccgcaaataa aagctacatg cctttctgac caattgctgg agaaaccctc tgacttgaac
      241 cttgaatcca atccgggaac caagctttta tagccttctt cattcagttt ggaatttaga
      301 aataagttga attcattcat aaaaatttgt ccatcaatgc gtccgtgaag ccattcattt
      361 gaaggccagc tcatatctgt tctgttacct tctctgattt catccgtaaa cggaaagaaa
      421 taaactataa ctgtatttgc actttgaagc cattccttcg gcatcataaa atgttctccg
      481 ataactgaag gtgattttaa taattgaaaa ccttcatcgt ctgcagctcc ataagcaaat
      541 attggttcat caaaaatttt caaccctgcc aactcttcat ttaatgcctt gtcatttgat
      601 acataattta tctctttttg cacaaattct gctgctaatc ttgcaattgt ttgtttatcc
      661 atgcttcccc ctttatagtt ttaaatttat atgctcctat tatataatat ttctcttctt
      721 atgtgttact taatttcata ttaaattcta acattcttat aattgtttaa aattattact
      781 aacgggtaac atttatttaa ttaaatgata gtgtgactat atagaggagt gtctgcttaa
      841 cactaccaaa aataacaagg agtacaaaac tatgataact tataataaac acatcgagta
      901 tctgcatgaa cttaagcgga agattatgct gcacagacat agattgacga ttatcatgat
      961 gctggtcatc ctgcttggaa caatggcatc cggctgcgcc ataggagaaa ccgacgttac
     1021 aaacccggac acaacaacag gagagtccat tgtggtaacg ataaccgacc cgcccgacga
     1081 gctggagccc atcgttgtgc cgactatgcc tgaagttatt ccaccctacc tcggtgctga
     1141 tccggagacc ggtctaacaa tgactgggac accgacattg gttgatttcg acacctatcg
     1201 gctcaaggtg agcggtaagg tcgaccaaga gctgagcctg gcctatgatg atttgcggcg
     1261 catgccaaag ctgactgcta ccccgacatt ggaa
//
LOCUS       DAOSOW010000448         1266 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_108732_length_1266_cov_3.180017, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000448 DAOSOW010000000
VERSION     DAOSOW010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1266)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108732_length_1266_cov_3.180017"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="PLD67_09345"
     CDS             complement(<1..956)
                     /locus_tag="PLD67_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HOT22769.1"
                     /translation="MQASQVVTLIIAAATMFGVIGFIVLLAHYYTLNGIKARTVGDGQ
                     HGTARFATKNEIKKTYAHVPYEPERWRKGINLPKVQGLVVGCKTAPGSVTALVDQGDV
                     HALMIGAAGVGKTANFLYPNLEYACASGMSFITTDTKGDLYRNYGCIAKDYYGYDVSV
                     IDLRNPTRSDGNNMLHLVNKYMDEYLANPDNLPAKARAEKYAKIIAKTIVNSGGLDAG
                     SYGQNAFFYDAAEGLLTAVILLVAEYCPKEKRHIISVFKLIQDLLTPSSVKGKNQFQL
                     LIEKLPSEHKARWFAGAALNTAEQAMQSDLSTALSRLNAFLDS"
     gene            complement(1049..>1266)
                     /locus_tag="PLD67_09350"
     CDS             complement(1049..>1266)
                     /locus_tag="PLD67_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22770.1"
                     /translation="NNQLKTSGIRTLEDKQKLIASYMSLALNCVKAQITICLESQQQK
                     IEALQKQNEERVDYMQAVCQEFLQHSI"
BASE COUNT          302 a          308 c          284 g          372 t
ORIGIN      
        1 gagtccagga atgcattcaa ccgggaaagg gctgtcgaca ggtcgctttg catcgcctgc
       61 tctgcagtgt ttaatgccgc tcctgcaaac catcttgcct tatgctccga cggaagcttc
      121 tcaataagca gctgaaactg gttttttccc tttacgctgg agggagtcag caagtcctgt
      181 atgagcttaa agaccgatat gatatggcgt ttctcttttg gacagtattc cgctaccaat
      241 aaaatgacgg ctgtcagcaa gccttcggct gcatcgtaga aaaatgcatt ctggccgtag
      301 gaacctgcat ccagtcctcc ggagttgacg atggtttttg caatgatttt tgcatatttc
      361 tccgccctgg cttttgctgg gagattatca ggattggcaa ggtattcatc catatatttg
      421 ttcacaaggt gcagcatatt gttgccatcg ctgcgggtag ggtttctcag gtcaatgacg
      481 gacacatcgt agccatagta gtctttggcg atgcatccgt aattcctgta aaggtcgccc
      541 ttggtatcgg tggtaataaa gctcatcccg gaggcgcagg catattccag attgggataa
      601 aggaaatttg cggtcttgcc gacccccgcc gcaccgatca tcaatgcatg cacatccccc
      661 tggtctacaa gagcggtgac cgagcctgga gccgttttgc atcccacaac cagaccctgc
      721 accttaggca ggttgatacc cttgcgccac cgttccggct catagggtac atgagcatag
      781 gtcttcttga tttcattctt cgttgcaaac cttgccgtac catgctggcc gtcacctacc
      841 gtcctggcct tgatgccatt caaggtatag taatgagcca aaagaacgat aaaaccgatg
      901 actccaaaca tagttgcagc agctatgatt aaagtaacca cctgtgatgc ttgcattggt
      961 ttcctccttc tttccaaatt aagaaagcca tgcaccaatg gtttttcatt cgcacatggc
     1021 tttcttcaaa tctatagtta tgatattgct aaatactgtg ctgcaagaat tcttggcaaa
     1081 cagcctgcat ataatcgact ctctcttcat tctgcttttg cagagcttct attttttgtt
     1141 gctgactttc gagacatata gtaatttgag ccttgacaca gttcagtgca agactcatat
     1201 acgatgcgat taatttttgc ttatcctcaa gggtacggat tccggatgtt ttgagctgat
     1261 tattaa
//
LOCUS       DAOSOW010000449         1251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_110769_length_1251_cov_2.585284, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000449 DAOSOW010000000
VERSION     DAOSOW010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110769_length_1251_cov_2.585284"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLD67_09355"
     CDS             <1..250
                     /locus_tag="PLD67_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HOT22771.1"
                     /translation="TFALATISIYLYINGKNYLVSLIPGTFYFFVTLSFILNAKIGFN
                     LPWTVAYSLAAVLCVAYIWFVRRRATVQREQIVQNVLS"
     gene            453..>1251
                     /locus_tag="PLD67_09360"
     CDS             453..>1251
                     /locus_tag="PLD67_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HOT22772.1"
                     /translation="MTTFIIGIIILIVGGYLYGSYCEKVFGPDDRETPAVTKADGVDF
                     VAMKKWKNSLINLLNIAGTGPVLGPIQGILFGPVAFITIPLGCVLAGSMHDYFSGMIS
                     MRNGGAQMPKLIKKYLGGKVFGVYNIIIWILMLLVGVVFVYTPGDLIVSELLKQEAVA
                     TNPTTWIVYAGIFLYYVVATIFPIDAIIGRVYPIFGAFLIISAVGIFFGILFGGGAYL
                     QNLSFEGMFSQHPGGLPFIPIFFITVACGILSGFHATQNTLIARTVSN"
BASE COUNT          380 a          203 c          244 g          424 t
ORIGIN      
        1 aacatttgca ttggcaacaa tatcaattta cctctacatt aacggcaaga attacttggt
       61 atccttgata cccggaacat tctacttctt cgttacatta agctttatat taaatgcaaa
      121 aatcggtttc aacctgccat ggactgtagc atattcattg gcagcagtat tatgtgtagc
      181 ttatatatgg tttgtaaggc gtagagcaac agttcaaaga gaacaaattg ttcaaaacgt
      241 attatcataa tttataagat ataaaaagct atctctcatt aaagagagat agttttttta
      301 ttggggacaa tcgcttaatt gcacagatgt ccctacaatt tcacgaaatt ggtatatttt
      361 gatttattga cgaaatttgt cgaatatgtt aatattataa tctaaaccta ggattagcag
      421 gtttagtact atattacttc ttaggaggag acatgactac ttttattatt ggaattatta
      481 ttcttattgt cggtggttat ttgtacggct catactgcga aaaagtattc ggacctgatg
      541 acagggaaac accggctgtt actaaggctg acggagtaga cttcgttgca atgaaaaaat
      601 ggaaaaactc cctgattaac ttactaaaca tagcagggac aggacctgta ttaggaccta
      661 ttcagggtat tttgtttgga cctgtggcat ttataactat tccgttagga tgcgttcttg
      721 caggctcaat gcatgactat ttctccggca tgatatccat gcgtaacgga ggagcacaaa
      781 tgcctaaatt aattaaaaaa tatttgggcg gaaaagtatt tggtgtatat aatattataa
      841 tctggatact gatgcttttg gtaggtgttg ttttcgttta tacaccggga gatttaattg
      901 tttctgaatt gctgaaacaa gaagctgttg caacaaatcc cacaacatgg attgtttatg
      961 caggaatttt cttatactat gttgttgcaa ctattttccc gatagatgca ataataggaa
     1021 gagtttaccc aatatttggt gctttcctga ttatatcggc agttggcatt ttcttcggta
     1081 tattattcgg aggaggagct tatttacaaa acttatcatt tgaaggtatg tttagtcagc
     1141 acccgggagg actgccattt attccaatat tctttataac ggttgcctgc ggtatattat
     1201 caggattcca tgcaactcag aataccttga ttgcaagaac cgtatcaaat g
//
LOCUS       DAOSOW010000450         1249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_111025_length_1249_cov_2.651591, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000450 DAOSOW010000000
VERSION     DAOSOW010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111025_length_1249_cov_2.651591"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="PLD67_09365"
     CDS             complement(<1..176)
                     /locus_tag="PLD67_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOT22773.1"
                     /translation="MNINWYPGHMKKTKELLLENIKLVNLIIEVVDARIPYSSKNPDF
                     DILFRDKKRLIVLN"
     gene            complement(256..600)
                     /gene="rplS"
                     /locus_tag="PLD67_09370"
     CDS             complement(256..600)
                     /gene="rplS"
                     /locus_tag="PLD67_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOT22774.1"
                     /translation="MDLIKSIENEQVKGVTPFNVGDTIKVHVKVKEGNRERIQIFEGI
                     VIKKQGESNRATFTVRKISYGVGVERTFPVHSPKIEKIEVVRRGKVRRARLFYLRERT
                     GKATKVKELRRY"
     gene            complement(699..>1249)
                     /gene="trmD"
                     /locus_tag="PLD67_09375"
     CDS             complement(699..>1249)
                     /gene="trmD"
                     /locus_tag="PLD67_09375"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOT22775.1"
                     /translation="GPGMLLRPEPMFRALEAIKKHNSYIVYLSPKGSLFNQNKAKELS
                     DKEHIILIPGHYEGIDQRIIDKYVDEEISVGDYVLTSGELPALMIIDAVGRLIKGVLG
                     SDESSKEESHSENLLEYPQYTRPESYMGLDVPEILLSGHHKNIEEWRLFKAIEITLKR
                     RPDMIKDKSIIEKYNELAKKYK"
BASE COUNT          332 a          264 c          168 g          485 t
ORIGIN      
        1 ttatttaata caatcagtct ttttttgtct ctaaacaata tgtcgaagtc aggattttta
       61 ctgctgtatg gaattcttgc atctacaact tcaatgatta aattaactaa tttaatattt
      121 tccagcaata actcctttgt ctttttcata tgtcccggat accagtttat attcataatg
      181 tacctctaat ctgaaaggga cacaccttat taaaggaatg tcccctttat atgaggacgt
      241 ccgccctcaa ttcaattaat accttctcag ttctttaact ttagttgctt tacctgttct
      301 ttctctcaag tagaacaaac gagcacgtct tactttacct cttcttacaa cttcaatttt
      361 ttcaatcttt ggtgaatgaa cagggaatgt tctttcaact ccaacgccgt aagatatttt
      421 tcttaccgta aaagtcgctc tgttgctttc gccttgtttt ttaattacga taccttcaaa
      481 tatctgaatt ctttctctgt ttccttcttt gacctttaca tgtactttta tagtatctcc
      541 aacattaaaa ggtgttacgc ctttaacttg ttcgttttct atagatttta ttaagtccat
      601 ttgtatacct cccttcgcct taacgttctt gttacctgta cagaggaccg ccgtatactt
      661 ttagcattat agcatatgaa tcataatgca tacaacattt atttatactt ctttgctaac
      721 tcgttgtact tttcaattat acttttgtcc tttatcatat cgggacgtct ttttaaagta
      781 atttctattg ctttaaacaa acgccattct tcaatatttt tgtgatgtcc cgataataat
      841 atttcgggca catctaagcc catgtagctt tccggtcttg tgtattgggg atattcgagt
      901 aaattttcgc tatgtgattc ttcctttgaa gattcatcag agcccaatac tcctttaatg
      961 agccttccta ccgcatcaat aatcattaat gcaggcagtt ccccgcttgt taagacatag
     1021 tctcccacag atatttcctc atcaacatat ttgtcgataa ttctttggtc tatgccttca
     1081 taatgtccgg gaataagtat tatatgctcc ttatccgaaa gctctttagc tttattctga
     1141 ttaaataaac tgccttttgg tgataaataa acaatatatg aattatgctt tttaatagct
     1201 tccaaagctc taaacatagg ttccggtctc aatagcatac cgggacctc
//
LOCUS       DAOSOW010000451         1247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_111239_length_1247_cov_5.473993, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000451 DAOSOW010000000
VERSION     DAOSOW010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111239_length_1247_cov_5.473993"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="PLD67_09380"
     CDS             <1..1223
                     /locus_tag="PLD67_09380"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF036799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOT22776.1"
                     /translation="IGAGTVVGGYLEAGKNEYEPPVVTLRPHRASSLLGVEIPVADML
                     KFLNTLEIESVYDGEKITSRIPYFRTDIEQEADLIEEVGRLYVFENIESKPLRGSTRV
                     GRKSDLRMVEDEIKSILTGMGLYEITTYSFISPKTYDSICAAEDSPLRNYVRILNPLG
                     EDFSSMRTTLMPNMLEVIQRNSNRGVEGAKLYEAGSIFIPRDQPVAEEPFEKLRLCIG
                     MYGSYDFYDLKGIIDAMLSRFGIKAALKKVSDNPTFHPGRTAALCVDDNIIGIYGEVS
                     PKVTDNYGLDHRVYLAEIDVEKILQYKNTEWKYEPLPKYPSMVRDIAVIVKDDVLAGD
                     MEEEIRKVNPYLIESVELFDVYKGEHIKEGYKSTAFSVTYRNKERTMKEKEVEKIHEK
                     ILQVLSNKFNAVLR"
BASE COUNT          436 a          196 c          290 g          325 t
ORIGIN      
        1 tgataggtgc aggaactgtg gttggaggtt accttgaagc agggaaaaat gagtatgaac
       61 ctcctgtggt aactttgaga ccccatcgtg caagttcgct tctgggtgtt gaaataccgg
      121 tagccgatat gcttaaattt cttaatactt tggaaataga atcagtatac gacggtgaaa
      181 agataacttc aagaatacct tatttcagaa cagatatcga gcaggaagcc gatttgatcg
      241 aagaagtcgg aaggctttat gtatttgaaa atattgaatc aaaaccgtta agaggaagca
      301 caagagttgg aagaaaatcc gacctgcgca tggtagagga tgaaatcaag tcgattctta
      361 ccggtatggg attgtatgaa ataaccacat attctttcat aagccccaag acctatgaca
      421 gtatatgtgc agcggaagac agccctttga gaaattatgt aagaatatta aatccgttgg
      481 gagaagactt ttcttccatg agaacaactc taatgccgaa catgcttgaa gttatacaaa
      541 gaaattccaa caggggtgtt gaaggagcaa aactatatga agcaggaagc atttttattc
      601 cgagggacca gcccgttgct gaagagccct tcgaaaagtt aaggctttgc ataggaatgt
      661 acggcagcta tgatttctat gacttgaaag gaattattga tgccatgcta tcgagattcg
      721 gaataaaggc agcattaaag aaagtatccg acaatccaac attccatccc ggaaggactg
      781 ccgcattatg cgttgatgat aatataatag gaatttacgg agaagtatcc cctaaggtta
      841 cggataatta cggacttgac cacagggtgt acttagcaga aattgatgtc gaaaaaattc
      901 ttcaatataa aaatacagag tggaaatatg agcccttgcc taaatatcct tcaatggtaa
      961 gagatattgc ggtaattgtg aaggatgatg tgcttgctgg tgatatggaa gaagaaatca
     1021 ggaaagtaaa tccttattta attgaaagtg ttgaattgtt tgatgtgtat aaaggggagc
     1081 atataaaaga aggatataaa agcactgctt tttctgttac ttacagaaac aaggagcgca
     1141 ccatgaaaga aaaagaagta gaaaaaattc atgaaaaaat ccttcaagtt ctttcaaata
     1201 aatttaatgc cgtcctaaga tgacaagggg acgtgacaag gggacgg
//
LOCUS       DAOSOW010000452         1246 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_111377_length_1246_cov_3.190596, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000452 DAOSOW010000000
VERSION     DAOSOW010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111377_length_1246_cov_3.190596"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLD67_09385"
     CDS             complement(<1..425)
                     /locus_tag="PLD67_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HOT22777.1"
                     /translation="MAKIINTPLTPELINDLMAGDRVFLNGIIYTGRDAAHKRLAELI
                     EKGEELPMDIKNQTIYYVGPCPAKPGQVIGSAGPTTSGRMDSYAPLLMEHGLKGMIGK
                     GIRNQQVVDSIIKYNAVYFAAIGGAGALLSEAIKEAEVIA"
     gene            complement(435..>1246)
                     /locus_tag="PLD67_09390"
     CDS             complement(435..>1246)
                     /locus_tag="PLD67_09390"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOT22778.1"
                     /translation="VEKLCIESNYYLPEDVKSALEDAMEKEESPLGKEILSDILKNQK
                     IARTKGVPICQDTGLAVIFLELGQEVRLVGGDLNEAINEGVRRGYLNGYLRKSAVDDP
                     FIERKNTGDNTPAIIHTKIVPGDKIKLIFAPKGGGSENMSALAMLKPSDGIKGIKEFV
                     LNTVEKAGANPCPPIIVGIGIGGTIEKTTLIAKQSLLRNVGEHNTNPKVAKLEEELLE
                     EINKLGIGPQGFGGRITALAVNIETFPAHIASMPVAINIQCHAARHREAII"
BASE COUNT          299 a          287 c          203 g          457 t
ORIGIN      
        1 gcaattactt ctgcttcttt tattgcttct gaaagcagtg ctccggcacc tccgatagcc
       61 gcaaaatata ctgcattgta cttgattatc gaatcaacca cttgttggtt cctgataccc
      121 ttgcctatca tacccttaag accgtgctcc attaataaag gtgcatatga atccattctt
      181 ccgctggttg taggtcctgc tgagccaatt acttgtcccg gtttagcagg acatggtccc
      241 acataataaa tggtctgatt cttaatatcc atgggaagct cttctccctt ttcaattaat
      301 tcagctaatc ttttatgggc tgcatctcgg cctgtataaa ttattccgtt taaaaaaact
      361 ctatctcctg ccataaggtc atttattaat tccggcgtta agggtgtatt tattattttc
      421 gccataacaa cctcctaaat tattgcttcc cggtgccttg cagcgtgaca ttgtatattt
      481 attgcaacag gcatgcttgc tatatgagca gggaaggttt ctatatttac ggcaagtgct
      541 gttattcttc ctccaaagcc ttgcggtcct atacccagct tattaatttc ttccagcaat
      601 tcttcttcta atttagctac ctttggattt gtattatgct ctcctacatt tctaagaaga
      661 gactgttttg caatcaaggt tgttttttct atcgttccgc caatacctat tcccactatt
      721 atgggagggc atggattagc gcctgctttt tctaccgtgt tcaatacaaa ttctttgatg
      781 ccttttatac catcagaagg ttttaacatt gcaagagcgc tcatattttc gctcccgccg
      841 ccctttgggg caaatattaa ttttatctta tctccgggta ctatctttgt gtgaataatt
      901 gctggagtat tatcgcctgt attttttctt tcgataaagg ggtcatcgac agcagacttt
      961 cgaagatatc cgtttaaata acctcttctt actccttcgt taattgcttc atttaaatct
     1021 ccgcccacca atcttacttc ctgtcccaac tcaagaaaaa taactgcaag tcctgtgtcc
     1081 tgacatatgg gaacaccttt tgtccttgct attttttgat ttttcagtat gtcagacagt
     1141 atttctttac ccaaaggaga ttcttctttt tccattgcat cttccaatgc actttttaca
     1201 tcctcaggaa gatagtaatt agattctata catagttttt ctactg
//
LOCUS       DAOSOW010000453         1245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_111535_length_1245_cov_2.450420, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000453 DAOSOW010000000
VERSION     DAOSOW010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111535_length_1245_cov_2.450420"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..81)
                     /locus_tag="PLD67_09395"
     tRNA            complement(8..81)
                     /locus_tag="PLD67_09395"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Arg,seq:cct)
     gene            complement(204..>1245)
                     /locus_tag="PLD67_09400"
     CDS             complement(204..>1245)
                     /locus_tag="PLD67_09400"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOT22779.1"
                     /translation="DLEGELLYDYNINQQLAIASISKLMTYTVMMDKVSAGEISLDDD
                     VVISGHAAATEGSNFGILAGETIKLRTLVEGMLIVSGNDCATAVAEYVGITQDNFVNM
                     MNTKASELGLLSASFINPHGLPINDEETGQNYMSVSDLYKLVQHVLRTYPEILEITKR
                     PELVITERNFSRAATNPILGVMEGADGLKTGYTDKAGICLVSTMPVKGNGIDYRLIGI
                     ILGAQTHEDRINKTIELLEYGKNNFIKLKLTDVSEAVDKVYISNSKSGKVNVYPASEF
                     NKIIKTQDFITTKITYNETVKAPLSKGEKIGTISILVNGEEIGQVDATVNENIEKANI
                     LVRIVRAFINLF"
BASE COUNT          358 a          263 c          184 g          440 t
ORIGIN      
        1 aaaaaagtgc acctgagagg aatcgaaccc ccgacacatg ccttaggagg gcactgctct
       61 atccttctga gctacagatg cacattattt ttaaatgtta caccatttta attgctttgt
      121 caatatcgtt gtttcaaacc gtttatgaca attaaaaacg gcttattgat attataaacc
      181 gataagtata aaatactgat atactaaaat aaatttataa aagctcttac tattctaacc
      241 aaaatattcg ccttttctat attttcattc actgtagcat caacctgtcc aatttcttct
      301 ccattgacaa gaatacttat tgttcctatt ttctcgcctt ttgacaatgg agccttcact
      361 gtttcattgt aagttatttt ggttgtaata aagtcttgag ttttaattat cttgttaaat
      421 tctgatgcag gatatacatt caccttgcct gactttgaat tggatatata aactttgtca
      481 actgcttcag agacatccgt taactttaat tttataaaat tgtttttccc gtattccagt
      541 aactcaattg ttttatttat tctgtcctca tgagtttgag ctcccaatat tattccaata
      601 agcctgtagt caattccgtt tcctttgacc ggcatagttg ataccaaaca aatacctgcc
      661 ttgtcggtat atcctgtctt caaaccgtct gccccttcca ttactcccaa tataggatta
      721 gttgcagctc tgctgaaatt tctttcggta ataacaagct ccggtcgttt tgtaatttcc
      781 aaaatctccg gatatgtgcg aaggacatgt tgcaccagct tgtacaaatc tgaaacagac
      841 atataatttt gaccggtttc ttcatcattt ataggtagac cgtgaggatt tataaagctt
      901 gccgataaaa gaccaagctc tgatgcttta gtattcatca tgtttacaaa attatcttga
      961 gtgattccta catattcggc tactgcagtt gcacaatcgt ttcctgatac tatgagcatt
     1021 ccctctacca gggttctcaa tttaattgtt tcacccgcta aaataccaaa attgctgcct
     1081 tccgtagctg cagcatgtcc gctgattaca acatcgtcat caagagaaat ttcacctgca
     1141 gaaactttgt ccatcatcac agtataagtc atcaacttgg aaatgcttgc aattgccaac
     1201 tgttgattta tattataatc atagagtagt tctccttcta aatct
//
LOCUS       DAOSOW010000454         1236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_112771_length_1236_cov_2.629128, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000454 DAOSOW010000000
VERSION     DAOSOW010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112771_length_1236_cov_2.629128"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="PLD67_09405"
     CDS             complement(<1..1196)
                     /locus_tag="PLD67_09405"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOT22780.1"
                     /translation="MAKKVAEAKRNDAMSIVIFMTDGMPTFRYSGTGSNTTNDYSGTN
                     TSRNEFNEAIEAAQNLKAYIGDEGEIYTVGLLNNVSSTSNQAKLASKLLSLNPMKNIR
                     NTNYSNIMNDVTDDNRWNPNTAVYAERYYPIHAGDDAAAKMTEIYEGIAGTINALANG
                     YVTDVIHKNFELTDDSKEYLEDQGVVITENDDGTTTLVFNNVKAYGEPNPLPVFEVKV
                     KDGVYGTGYTNEEAYYTFTFFGDEVSKTKYFEQPIVAIKPTAANDDGYETFQDQVLTV
                     LLAQSILVNDETVKIEDGDYSVSNLVVKDEYVTTINTNKGGTAVVAADGTFVYTPPSE
                     FTGDDTFVYKNKAVVTYTGIGADVFGLAGDYISNEATVTITVKPITGQLIPYTIQHLV
                     QESNEV"
BASE COUNT          330 a          256 c          208 g          442 t
ORIGIN      
        1 tgaacttcat ttgattcctg cacaagatgt tgaatcgtgt aaggtatgag ctgtcccgta
       61 atcggcttaa cagtaattgt tacggttgct tcattggaaa tataatcacc ggcaagaccg
      121 aatacatccg ctcctattcc cgtatatgta accactgctt tgtttttgta tacaaatgtg
      181 tcatctcccg taaattcgga cggcggagta tatacaaaag ttccgtcggc agctactact
      241 gcagtaccgc ccttattagt atttatagtt gttacatatt catctttaac aactaaatta
      301 ctaaccgaat aatcaccatc ttctatttta acggtttcat cgtttacaag gatactttgt
      361 gcaagcagaa ccgttaatac ttggtcttgg aatgtttcat atccgtcgtc attagctgcc
      421 gtaggtttaa ttgcaactat aggttgttcg aaatactttg tcttagatac ttcatctcca
      481 aaaaaagtga atgtataata tgcctcttca tttgtatagc ctgttccgta aacaccgtct
      541 ttaactttta cttcaaagac aggcagcgga ttaggttctc catatgcctt tacattattg
      601 aaaaccaatg tagtggttcc atcatcattt tccgttatta caactccctg atcttcaaga
      661 tattcttttg aatcatcagt cagttcgaaa tttttatgta taacatcagt aacatatccg
      721 tttgccaaag catttattgt gccggcaata ccttcatata tttcagtcat cttagcagca
      781 gcatcgtctc ctgcatgaat cggataatat ctttcggcat atactgctgt attggggttc
      841 cacctgttgt catcggtaac atcgttcatt atgtttgaat agttagtatt tctaatattt
      901 ttcataggat tcaaacttaa caatttagaa gctaacttgg cttgattgct tgtgcttgaa
      961 acattattga gcaatccgac ggtatatatt tccccttcat cgccgatata tgcttttaag
     1021 ttttgtgctg cttcaattgc ttcattaaat tcattccttg aagtattggt ccccgaataa
     1081 tcgttggtag tattgcttcc tgtaccggaa tatctaaaag taggcatacc atccgtcata
     1141 aagataacta tgctcatggc atcatttctt ttagcttcgg caaccttttt tgccatcaag
     1201 aatccgcctt cggtattggt tccttcatta gaggtg
//
LOCUS       DAOSOW010000455         1229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_113757_length_1229_cov_2.484668, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000455 DAOSOW010000000
VERSION     DAOSOW010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1229
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113757_length_1229_cov_2.484668"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..1165)
                     /gene="mraY"
                     /locus_tag="PLD67_09410"
                     /pseudo
     CDS             complement(191..1165)
                     /gene="mraY"
                     /locus_tag="PLD67_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
BASE COUNT          374 a          265 c          184 g          406 t
ORIGIN      
        1 tcaacttcta tatcttctac ttcttttaat ctgtctctta atttctcctc gtttgaatca
       61 tcaaatgcat aaatattcgc acctgtctta cttaatcctt ttatgcaggc aatgcccgaa
      121 actcctaatc ctaccacaag tacattcatt ttttaccttc cttataatgc ccattattat
      181 cttatgttgt ttatattgaa taaataccta taaatgccaa tatcagagta gcaatccaaa
      241 aggccgacac tacctttgtt tccttccacc cgcacatttc gaaatgatgg tgcaaaggtg
      301 ccattttaaa tactcttctt cttgttctct tataatggaa aacctgaata ataacttaaa
      361 gtgcttcaac aaagtatatg ccgccaatta tgggtataaa taaaaccatg ccggataaaa
      421 cggcaactga tgaaacaaca cctcccaaag ccaatgagcc tatatctccc ataaatactt
      481 tggcaggatt tgcgttatgt tttaaaaatc caaggcaggc accgcttgtt gcggcacata
      541 tcaatgatat tccttcatat atttgatttg tttcttttaa actgtttgct atcaaactga
      601 aagcggccat aactatcaat gttactccgg tgttaagacc gtcaagtcca tcggtcaaat
      661 tcacggcatt gacagtacct acggcaataa atgtaatgaa gggtatgtac aaaacaccta
      721 aatcaatgtg catattaacg aatggaatca ttaatgcagt tttcatggcg ctgtatcttg
      781 agccgtacca agcaagcagg acggaaaaaa tcagctgtcc cacgattttt tgatacggtt
      841 ttaatccttc attttgtctc attaccacct ttttataatc atctatggct cctattaacc
      901 caaaacccag catgaagaat attacagcca gtacttcttt tgtaatgagc cttccggtga
      961 taatcgaagt taaaattgct aatattataa taattccgcc cattgtaggg gtacccgact
     1021 tggataaatg acttttaggt ccgtcctctc gtatactttg tcctacttta aggcgtttta
     1081 agaccggaat tacaacgggt cctaagaaca atgctattaa aaaggatact attattactc
     1141 ttattatttg tccgttacct tgcataataa ctcctaattc attggttata tcaattgtca
     1201 ttgctatgaa aaagctacgt gtcattctg
//
LOCUS       DAOSOW010000456         1222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_114644_length_1222_cov_3.809769, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000456 DAOSOW010000000
VERSION     DAOSOW010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114644_length_1222_cov_3.809769"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..845)
                     /locus_tag="PLD67_09415"
     CDS             complement(51..845)
                     /locus_tag="PLD67_09415"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HOT22781.1"
                     /translation="MFGYVTINKMELKFKEYYSYKGYYCGLCKCLKTMYGNKSRLTLN
                     YDMTFLILLLSSLYEPENKIYNERCMIHPKNKQLIIQNEITEYAASLNVLLSYYNMLD
                     NWVDDRDYKSLAAAKILQSQLQKSVSNLNDITKTIKLRLNNISKLEKEDTEDIDAVSN
                     EFGHLMEEIFLYKKDHWEQSLRKIGFYLGKYIYFIDAYEDMKKDLENKSYNPFNKLNI
                     ENKEEYANNLLMLNLSFLSDEIEKLPLVRDKGIIDNIIYSGMLNKF"
BASE COUNT          396 a          195 c          165 g          466 t
ORIGIN      
        1 ttttttatta gattttatcc acagttatca caaaatcatt ttacaattgc ctaaaattta
       61 ttcaacatgc cggaatatat aatattatct attattcctt tgtccctgac taagggtaat
      121 ttttcgattt catcgcttaa aaatgacaaa ttaagcatta aaagattatt agcgtattct
      181 tccttgtttt caatatttaa tttattaaat ggattatatg atttattttc taaatccttc
      241 ttcatatctt cataagcatc tatgaagtaa atatattttc caagatagaa acctattttt
      301 cttaagcttt gctcccagtg gtctttttta tataaaaata tttcttccat caaatggccg
      361 aattcgttgg atacggcatc gatatcttcc gtgtcttctt tttcaagctt tgaaatgtta
      421 ttaagcctca gtttaattgt ttttgtaata tcattaagat tagatacgga tttttgtaat
      481 tgactctgca atattttagc cgctgccaat gacttgtaat ccctgtcatc aacccagtta
      541 tcaagcatat tgtaatatga aagcaagaca tttaaagaag cggcatattc tgttatttca
      601 ttttgtataa taagctgctt attcttggga tgaatcatgc acctttcatt ataaatttta
      661 ttttcgggct catagaggga gcttaacaaa agtattaaga aagtcatatc ataattcaat
      721 gtcagccttg atttattccc gtacattgtt tttaaacatt tgcacagacc gcagtaataa
      781 cccttgtaac tgtaatattc cttgaatttt aattccattt tatttatcgt tacgtaaccg
      841 aacatttact cactcatttc tatgttgtta ttaaattgta tcaattcctc cttgcttttt
      901 caatacaaat tttaacctca gagagctaag aattatcacg tatttacaac gccacgtaat
      961 aaatacgtct tgttaccctg agcgtaagcg aaagatttgt taattataaa aaatgttaat
     1021 aaattataca attgacattc atgtatttgg ataatattat atataaatac aaataatgaa
     1081 gcatagaatt agcttttgca tttaaataat cattgtgtct tgtatagcaa gcaataacag
     1141 cttgcactta ggcacttacg atagaatgag tatccgggga ggtagaaatg aatacggtta
     1201 aaaaagagat aaccagtgct tt
//
LOCUS       DAOSOW010000457         1214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_115846_length_1214_cov_2.196721, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000457 DAOSOW010000000
VERSION     DAOSOW010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115846_length_1214_cov_2.196721"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="PLD67_09420"
     CDS             <1..1131
                     /locus_tag="PLD67_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HOT22782.1"
                     /translation="HEGKTVEEVKQKFEEAFTGVSASEISEAEQALINEGLPVSEVQR
                     LCDVHSAVFKGSIEEIHRETDPMKIPGHPANVLYLENRRLERIIEEKIKPYLEKGKES
                     LEAIQNGLEELSVIDIHYSKKENLLFPYMEKYGITAPPKVMWGVDDEIRDDLKTLKSD
                     LSYGVFNDELKKGVEELINRINEMIFKEESIMLPMLLDIMTQDEWKKVADDSSEIGNI
                     IDFVPVWNPDKEAKKEYKEDIKEPGAIILPTGVFNTEELTHMLNTLPIDITFVGSDDK
                     VKYFSQSSERIFARTKTIIGRNVSNCHPPASVHIVEGIVEDFKSGKKDHEDFWIKMGD
                     KYVYIRYFAVRNEEGKYLGVVEVTQNIKPIQEITGEKRLVED"
BASE COUNT          434 a          183 c          278 g          319 t
ORIGIN      
        1 catgaaggta agactgtcga agaggtaaaa caaaagtttg aagaggcttt tacaggggtt
       61 tcggcttcgg aaatatccga agcagaacag gcattaatca atgaggggtt gcctgtttcc
      121 gaagtgcaga ggctgtgtga tgttcattcg gctgttttta agggctccat agaagaaatc
      181 catcgggaaa cagaccctat gaaaattccc ggtcatcctg ccaatgtatt gtatcttgaa
      241 aacaggagat tagaaagaat tatcgaagaa aaaatcaaac cttatttgga aaaaggaaaa
      301 gaatctcttg aagctattca aaatggctta gaagagttgt cagttataga catccattat
      361 tccaaaaaag aaaatctttt gtttccttac atggaaaagt acggaataac cgctccgcct
      421 aaggttatgt ggggtgtgga cgatgaaata agggatgatt tgaaaacgct taaatccgat
      481 ttatcctatg gtgtatttaa cgatgagctt aaaaaaggtg ttgaagaatt aatcaataga
      541 ataaatgaaa tgatattcaa ggaagaaagt ataatgcttc ctatgctttt agatataatg
      601 actcaggatg aatggaaaaa ggttgccgat gacagcagcg aaataggcaa cataatagat
      661 tttgtacctg tttggaatcc tgataaggaa gcaaaaaagg agtataaaga agatattaag
      721 gagccgggag ctataattct tccgacaggt gtttttaata cggaagagct tactcatatg
      781 ctcaatactc tgccaattga cattacattt gtggggagtg acgataaagt aaaatatttt
      841 tcacaaagca gtgaaagaat atttgctcgg acaaagacta ttataggcag aaacgtttca
      901 aactgccacc cgccggcaag tgtacatata gtagaaggaa ttgtggaaga ttttaagtca
      961 ggcaaaaaag accacgagga tttctggatt aaaatgggcg ataaatatgt ctatatacga
     1021 tattttgcag tcaggaatga agaaggcaag tatcttggag ttgtggaagt aacccagaat
     1081 attaagccta tacaggaaat cacgggagaa aaaagactgg tggaagacta atacaccctt
     1141 catgtcatcc tgagcgtaag cgaaggatct cataattatt gaaaacatga gattcttcag
     1201 gctgcgcctt caga
//
LOCUS       DAOSOW010000458         1210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_116392_length_1210_cov_4.076190, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000458 DAOSOW010000000
VERSION     DAOSOW010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116392_length_1210_cov_4.076190"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="PLD67_09425"
     CDS             complement(<1..129)
                     /locus_tag="PLD67_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HOT22783.1"
                     /translation="MEKDKALELAIAQIEKQFGKGSIMKLGENAKLNIEAIPTGALP"
     gene            complement(196..759)
                     /gene="pgsA"
                     /locus_tag="PLD67_09430"
     CDS             complement(196..759)
                     /gene="pgsA"
                     /locus_tag="PLD67_09430"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOT22784.1"
                     /translation="MKIVDWFKNLNLPNKLTVIRVISIPLFLLFLYISKGILRFLPLL
                     IFAGAAVTDLVDGYIARRDNLVSDFGKFMDPLADKLLTASAFIAFVELGYISAWVVVL
                     IISREFLVSGFRTLAASKGVTIAANAWGKIKTVFQMVLIIAILLNYTGYFGFTNTIIA
                     PLIAITVLLTITSGAVYINENIGVIKN"
     gene            complement(737..>1210)
                     /locus_tag="PLD67_09435"
     CDS             complement(737..>1210)
                     /locus_tag="PLD67_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOT22785.1"
                     /translation="VEVKDAVIRTSLITGFPGETWDEHEELKNFLKEYRLDRVGIFKY
                     SREEDTPAYKLPGQIDEDVKEQRLNELMEIQQEISYENNLLKIGKTYDVLIEEKDAEN
                     VYVGRTYMDSIEIDGCVYVASNKELRLGEIYKVRINDALEYDLIGDVSDEDSRLV"
BASE COUNT          375 a          260 c          155 g          420 t
ORIGIN      
        1 aggcagtgca ccggttggta ttgcttctat atttaatttc gcattttctc caagcttcat
       61 tatagaacct ttaccgaatt gcttttctat ttgagcaatg gcaagctcaa gtgccttatc
      121 tttttccata tccgactcct ttagaacatt tgttcacctt tgattataca tagaataata
      181 tgtattgtca acaatttaat tttttataac accaatattt tcattaatat atactgctcc
      241 cgaagttatg gtcaagagta cggttattgc aattaaagga gctattatag tatttgtaaa
      301 accaaaatat cctgtatagt ttaataaaat tgcaataatt aataccatct ggaacactgt
      361 ttttatttta ccccatgcat tggcagcaat agtgacacct tttgatgcag ccagtgttct
      421 gaaaccggaa accaaaaatt cccttgatat aatcaataca acaacccatg cacttatata
      481 tccgagctca acaaaagcta tgaaagcaga agccgtcaaa agcttgtctg caagagggtc
      541 cataaatttt ccaaagtcag atactaaatt atctctcctt gcaatgtatc cgtcgactaa
      601 atcagtaaca gccgcgcctg caaagattaa cagcggcaga aaccttaaaa ttcctttgct
      661 tatgtataag aataacagaa ataaaggtat tgaaattact cttataacag taagtttgtt
      721 tggtaaattt aaattcttaa accaatctac tatcttcatc actcacatct cctatcaaat
      781 catattctaa tgcatcatta attctgactt tataaatttc tcccaacctt aattctttat
      841 ttgatgctac ataaacacag ccgtcaattt caatcgaatc catgtaagtt ctccccacat
      901 agacattttc cgcatccttc tcctcaatca atacatcata ggtcttaccg attttcaata
      961 aattattttc gtaagatatc tcttgttgga tttccattaa ctcatttagt ctttgctctt
     1021 ttacatcttc gtctatctgt cccggaagct tgtatgccgg tgtatcttct tcccttgaat
     1081 atttaaaaat cccaactcgg tcaagcctgt attctttcaa aaaattcttt aattcttcat
     1141 gctcatccca agtttctccc ggaaaaccgg ttattaaaga tgttcgtata acggcatctt
     1201 taacttccac
//
LOCUS       DAOSOW010000459         1195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_118593_length_1195_cov_2.385088, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000459 DAOSOW010000000
VERSION     DAOSOW010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118593_length_1195_cov_2.385088"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            370..>1195
                     /locus_tag="PLD67_09440"
     CDS             370..>1195
                     /locus_tag="PLD67_09440"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOT22786.1"
                     /translation="MRSKLVLLLAGIMLVTSIFGGCTAKEPAAAETPEAPEAAEAPKA
                     VETVKIGAILPLTGGAAATGVKLQAAMEVAQEIINGEHPEIAIPLAKEAGLPNLGNAK
                     LEIVFADHQGNPEMAKSEAERLIEEGVAGLVGSYQSSATKPASQAAEQYGVPFVAGSS
                     SSAALTERGLQYFVRIAPNDDMETAMFFDYLIYLNENFNGGIKKIAVAYIDNEFGVHA
                     KDMVEKWIKEKYGALGFELVGTVPYAVDVTNVDTEVQQIKSLNADAIFHASYIADIT"
BASE COUNT          339 a          239 c          291 g          326 t
ORIGIN      
        1 aaattgatac acctgtgaaa aacctttata taggctcctg ctgggcgatg cagattggcg
       61 gtatacccgg tgcccttgca gccgcctacc agtgtgccag caggataagc tgaggcactc
      121 aataagtata gacggatagt taacagctca ttaaggaata aataccggac attaaattat
      181 ttcattgtcc ggtattttta ttttttgacc ttcgagatga cctgtcagga caaggatgat
      241 attttgttga aattgagtct aaatccaact ttatagtaca taaatatata aaagagctta
      301 tgtgcaagct caattgagat aaatatgttc agctttgctg gatacctaaa aaattcggag
      361 gtggagaaaa tgagatccaa gttagtactt ttacttgcag gtatcatgtt ggttacctct
      421 atttttggcg gctgcactgc caaggagccg gctgctgctg aaacgcctga ggctcctgag
      481 gctgctgaag ctcctaaagc cgttgagaca gtcaaaatag gtgccatact gcccttgacc
      541 ggcggtgctg ctgctaccgg agtcaagctc caggctgcca tggaggttgc acaggaaatt
      601 atcaacggtg aacatccgga aatagctatt cccttggcaa aggaagccgg gctgcctaac
      661 ttaggcaatg caaaattaga aatcgtattt gccgatcatc agggtaatcc tgaaatggct
      721 aaatccgaag cggagagact tattgaagaa ggcgtagcgg gtttggtcgg atcttatcag
      781 agctcggcta caaagccggc aagtcaagct gcggagcaat atggcgtgcc cttcgttgca
      841 ggctcttctt catcagctgc tttgacggag agaggacttc agtattttgt tcgtatagct
      901 cccaatgacg atatggaaac agcaatgttc tttgactacc tgatatatct taacgagaat
      961 ttcaacggcg ggattaagaa aattgcagtt gcttatattg ataatgaatt cggagtccat
     1021 gcaaaggata tggttgagaa atggattaaa gagaaatatg gcgctctcgg ttttgagctt
     1081 gtcggtactg tgccttatgc agttgatgtc accaatgtcg ataccgaagt acagcagata
     1141 aagagcctga atgccgatgc catattccac gcttcctata ttgcagatat tacta
//
LOCUS       DAOSOW010000460         1171 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_122287_length_1171_cov_2.135305, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000460 DAOSOW010000000
VERSION     DAOSOW010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122287_length_1171_cov_2.135305"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="PLD67_09445"
     CDS             complement(<1..528)
                     /locus_tag="PLD67_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOT22787.1"
                     /translation="MHIDIDGLNINYIDEGKGRHVLLLHGWGGSIQTMMPIFNILKEK
                     FRVVALDLPGFGNSDIPKEPWNSYDYAECINKFIEELNLNEIILFGHSHGGRISIVLA
                     SKYNFVKKLILIDSAGIIPKRKAKYYIKVYWFKMLKKIYLTFPLKNKQERLDKFYKKF
                     GSRDYRDAEGVMRQTM"
     gene            complement(830..>1171)
                     /locus_tag="PLD67_09450"
     CDS             complement(830..>1171)
                     /locus_tag="PLD67_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HOT22788.1"
                     /translation="MIVPGLLAVVVPLIVGFLLGKEALGGLQAGALLTGVLMAIFMSN
                     AGGAWDNAKKYIEGGTHGGKGSPAHQAAVVGDTVGDPFKDTSGPSINILIKLITVVSL
                     IFGPLLGSGIL"
BASE COUNT          361 a          254 c          170 g          386 t
ORIGIN      
        1 cattgtctgc cgcattactc cttctgcgtc tctgtaatcc ctagagccga attttttata
       61 aaatttatcc aacctttctt gtttattttt taaaggaaaa gttaaataaa tttttttaag
      121 cattttaaac cagtatactt ttatataata tttagctttc ctcttgggaa taattcctgc
      181 actgtctata agtattaatt tcttaacaaa attgtatttg cttgctaaaa ctattgaaat
      241 cctgccgccg tgagaatgac caaataaaat aatctcattt aaattcaatt cctcaatgaa
      301 tttattgata cattcggcgt aatcatagga attccacggc tctttcggaa tatcactatt
      361 accgaagccc ggtaaatcta atgctacaac tctgaatttt tctttcagta tattaaaaat
      421 cggcatcatt gtttgtatgc ttccgcccca gccatgcagc agcaagacat gtctgccttt
      481 tccctcgtct atgtagttga tatttaatcc gtctatatct atatgcatat acatcccctc
      541 atcctgtcaa cctgagcgca gcgaaggttc tcatgagatt cttcgggcta aagccctcag
      601 aatgacaaaa atgttattta tataaatgac atatattaaa gccctcataa tgacaacatt
      661 attaatatcc agtacttata ctattatatg tcaatttatt gaaatgatta taccatctaa
      721 acagtacatc tgcaagaaaa aagacctctt taaaaaggtc aaagaataat gactatagac
      781 attggtgcca ggcacctcta aggtgcctgg caccgggaat aattttatat tatagtattc
      841 cgcttcctaa caatggaccg aatattaatg aaacaacagt tatcagttta atcaatatat
      901 ttattgaagg tccggaggta tctttaaatg ggtctcctac cgtatctcct acaactgctg
      961 cttggtgagc cgggcttcct ttgcctccat gtgtaccgcc ttcgatatat ttctttgcat
     1021 tgtcccatgc tccacctgca tttgacataa atattgccat aagaacaccg gttaataaag
     1081 cacctgcctg taatccgcca agagcctctt tacctaataa aaatcctact atcaaaggaa
     1141 ctacaaccgc taagagcccc ggaactatca t
//
LOCUS       DAOSOW010000461         1154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_125045_length_1154_cov_3.189263, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000461 DAOSOW010000000
VERSION     DAOSOW010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_125045_length_1154_cov_3.189263"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /gene="purD"
                     /locus_tag="PLD67_09455"
     CDS             complement(<1..711)
                     /gene="purD"
                     /locus_tag="PLD67_09455"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOT22789.1"
                     /translation="MKILVVGSGAREHTICWKLKQSSKLSKLYCAPGNAGISKIAECV
                     DIKADELEKLVEYSVKNSIDLVVVGPEVPLVAGLVDMLNEKGIKAFGPAKAGAKFEGS
                     KSYSKDFMKKYSIPSANYEIYTDSEKAISELNKFNTPIVVKADGLAAGKGVLICETHE
                     EAKEAIKSIMVKKLFGEAGNTVVIEEFLKGTEASLLCFVDGKNMIPMESARDYKKAYD
                     QDKGLNTGGMGCFSPNTIF"
     gene            complement(720..>1154)
                     /gene="purH"
                     /locus_tag="PLD67_09460"
     CDS             complement(720..>1154)
                     /gene="purH"
                     /locus_tag="PLD67_09460"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494649.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOT22790.1"
                     /translation="QEIDSKLLPEGEELKTVTSSKPTEKEMKDLFMAWKIVKHTKSNA
                     IVIVKDGQSLGIGPGQVNRIWACKQAIEHAYDFLGKDSTKGASLASDAFFPFPDCAEE
                     AAKAGIRAIIQPGGSLKDDESIEVCNKYNISMVFTGMRHFKH"
BASE COUNT          285 a          272 c          192 g          405 t
ORIGIN      
        1 aaatatggtg tttggagaaa agcatcccat tcctcccgta ttgagccctt tgtcctggtc
       61 atatgctttt ttgtaatctc ttgcactttc catgggaatc atatttttgc catcaacaaa
      121 gcatagtagc gaagcttccg taccttttaa gaattcttct ataacaactg tatttcctgc
      181 ttcaccaaag agctttttaa ccattatact tttaatggct tctttggctt cctcatgtgt
      241 ttcacatatt aaaacgcctt tacctgcagc taaaccgtca gcctttacaa caatcggagt
      301 attaaatttg ttaagttcac tgattgcttt ttcactatcc gtataaattt cataattagc
      361 agatggtatt gagtattttt tcatgaaatc ctttgaatag gatttgctcc cttcaaattt
      421 tgctccggct tttgcaggtc cgaatgcctt tattcctttc tcgttaagca tgtcgaccaa
      481 ccctgctacc aatggcactt cagggccgac aactaccaaa tcgatactgt tttttaccga
      541 atattcaact aacttctcca gttcatctgc tttaatatca acacattctg caattttaga
      601 tatgcctgca tttccgggag cacagtataa cttggacagt ttgctgcttt gttttaattt
      661 ccagcaaatt gtatgctctc ttgctccgct tccaactaca agaattttca taagacctcc
      721 taatgtttaa agtgtctcat accggtgaaa accatggata tgttgtattt gttgcacact
      781 tctattgatt catcatcctt taatgagccg cccggctgaa taatagctct tattccggcc
      841 tttgccgctt cctccgcaca atcggggaac gggaaaaatg catccgatgc aagggatgct
      901 cccttggtgc tgtcttttcc caaaaaatca tacgcatgct caattgcctg cttgcatgcc
      961 catatgcggt ttacctgtcc cggcccgatt ccaagggact gtccgtcttt aacaatgact
     1021 atggcattgg attttgtatg ctttacaatt ttccatgcca taaataaatc cttcatttcc
     1081 ttttcggtcg gtttagagga agttactgtt tttaactctt caccttcagg aaggagcttt
     1141 gaatcaattt cctg
//
LOCUS       DAOSOW010000462         1131 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_129063_length_1131_cov_1.861524, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000462 DAOSOW010000000
VERSION     DAOSOW010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129063_length_1131_cov_1.861524"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PLD67_09465"
     CDS             <1..946
                     /locus_tag="PLD67_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HOT22791.1"
                     /translation="SRTERQWKQVNKSQNNDTSYWIDKYNLSMEMDTWTYPLHFIDFE
                     TSMAAIPFNKGRRPYEGIAFQFSHHIVHKNGDIEHRGQYLNTEPGVFPNYEFVRKLKA
                     ELDKDEGTIFRYAAHENTYLNTIYRQLKEDENYIPDREELCEFIKSITKSVNGSFEKW
                     EGPRNMVDMCELVKRYYYDPAMKGSNSIKAVLPAILNSSAFLRDKYSKPIYGTKEIPS
                     LNYNNWTWIKYENNKVIDPYKLLPKMFEDVSDKDFILLNNDQVRDGGAAMTAYAMLQF
                     TEMSDYERNEIKKALLKYCELDTFAMVMIYEGWKDMIR"
     gene            1029..>1131
                     /locus_tag="PLD67_09470"
     CDS             1029..>1131
                     /locus_tag="PLD67_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016475448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase"
                     /protein_id="HOT22792.1"
                     /translation="MIGAIIGDIVGSRFEFNNHRNKEFELFTDKCNVT"
BASE COUNT          405 a          173 c          229 g          324 t
ORIGIN      
        1 ttcaaggaca gagagacagt ggaagcaggt aaataaatca caaaacaatg atacttcata
       61 ttggattgac aaatataatt tatcaatgga aatggataca tggacctatc ctcttcactt
      121 cattgatttt gaaacttcca tggctgccat tccgtttaat aagggaagaa gaccctatga
      181 agggattgct tttcagtttt cccatcatat tgttcacaaa aacggagata ttgagcatcg
      241 gggacaatat ttaaacacag agcccggagt atttccaaat tatgaatttg taagaaaatt
      301 aaaagcagaa ttagataagg atgaagggac aatttttaga tatgccgccc acgagaatac
      361 atatttaaac actatttata ggcaattaaa agaagatgaa aattatattc ctgacaggga
      421 agaattatgc gaatttataa agagtatcac caaatcggtg aacggtagct ttgaaaagtg
      481 ggaaggtcct cgcaatatgg ttgacatgtg cgagcttgtt aaaagatact attatgaccc
      541 ggcaatgaaa ggctcaaatt caataaaggc agttcttcct gccattttaa acagctccgc
      601 ctttttacgg gacaagtatt cgaagcctat ttacggaacg aaagaaattc caagcttaaa
      661 ctacaataat tggacgtgga ttaaatatga aaacaataag gttatagacc catataagct
      721 tctgcctaaa atgtttgagg atgtttcaga caaagatttt atattattga ataatgacca
      781 agtaagagac ggcggtgcag ccatgaccgc ctatgcaatg cttcagttta ccgaaatgtc
      841 ggactatgag cgcaatgaaa ttaaaaaggc tctgcttaaa tactgtgaat tggatacatt
      901 tgcaatggtt atgatttatg aaggatggaa ggatatgatt cgttaatatc aaatgtcgca
      961 atttaagcga ccttacctag aaatttcact ttatactgtt aatagtatag agaaagaagg
     1021 tgtattagtt gataggagcg attataggcg atattgtagg ttctcggttt gaatttaata
     1081 atcacaggaa caaggaattt gaacttttca ccgataaatg caatgtaaca g
//
LOCUS       DAOSOW010000463         1125 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_129974_length_1125_cov_3.175701, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000463 DAOSOW010000000
VERSION     DAOSOW010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1125
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129974_length_1125_cov_3.175701"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PLD67_09475"
     CDS             complement(<1..711)
                     /locus_tag="PLD67_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOT22793.1"
                     /translation="MLTLKNVSAGYNGIDVINNISLCVKEGENLCIIGPNGCGKTTLI
                     KAISGIIPHTGIIKIDGTDINNMKREQVAKKIAVMSQISTIYFSYSIYETVLLGRYLH
                     MKGRTFKEPSAKDREYTDKCLKAVGLFNIKHKQISTLSGGQLQRVYLARTLAQEPNII
                     LLDEPTNHLDLKHQTELIDFLKDWSKYEGHSVIGVMHDLNLAIKLSDNMLVLDNGNIA
                     AYGSTNEIISSNLLNEVYD"
     gene            complement(717..>1125)
                     /locus_tag="PLD67_09480"
     CDS             complement(717..>1125)
                     /locus_tag="PLD67_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOT22794.1"
                     /translation="FPIVLIGITVLQNCSKEMDIMTFGEEQAFTMGVDVHKVKWILLS
                     LSAALTGSAIAFVGVIGFIDLIAPHVVRKIFGSNHKTVIPMSVFFGGSFMVLCDLIAR
                     TIISPSELPVGAITALIGAPFFAYVYFSRRKGL"
BASE COUNT          347 a          231 c          174 g          373 t
ORIGIN      
        1 atcatatact tcatttaaaa gatttgatga aataatttca tttgtactgc cgtaagcagc
       61 aatatttcca ttatctaata ccagcatatt atcagacaat tttattgcca aattcaaatc
      121 atgcattaca ccgattacag aatggccttc atattttgac caatccttca aaaagtcgat
      181 taactctgtc tgatgtttta aatccaaatg atttgttggc tcatcaagaa gtataatgtt
      241 tggctcttga gctaaggttc ttgccaaata aaccctttga agctgccctc ccgacaaggt
      301 gcttatttgc ttatgcttaa tattaaataa acctacagct tttaaacatt tatccgtata
      361 ttctctatcc tttgcggaag gctctttaaa tgttcttccc ttcatatgaa gatatcttcc
      421 gagaagtacg gtttcgtaaa tgctgtatga aaagtaaatg gttgaaattt gggacataac
      481 agctattttc tttgcaactt gctccctttt catattatta atatcagttc cgtctatttt
      541 aataattccc gtatgaggaa taattcctga aattgcctta ataagtgtag tctttccgca
      601 accgttggga ccgataatgc aaagattttc accttcctta acgcataagg aaatattatt
      661 tattacatca attccgttat aacctgcaga tacatttttt aaagtaagca tagcatctat
      721 aacccctttc ttctcgaaaa gtatacataa gcaaaaaacg gtgcacctat taatgccgta
      781 attgctccca ccggcaattc agaaggcgat ataatcgttc tggcaatcaa gtcgcataga
      841 accatgaatg agcctccgaa aaacactgac atcggtatta ctgttttgtg attggagccg
      901 aatatttttc ttaccacatg tggtgcaatt aaatcaataa atccgattac tcccacaaat
      961 gcaatggcac tgcctgtaag tgccgctgac aggctgagaa gaatccattt gactttgtgg
     1021 acatccactc ccatggtgaa tgcctgctcc tctccaaaag tcattatatc catttctttt
     1081 gaacaatttt gcaatactgt aattcctatt aaaacgatgg gaaag
//
LOCUS       DAOSOW010000464         1116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_131668_length_1116_cov_2.283695, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000464 DAOSOW010000000
VERSION     DAOSOW010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_131668_length_1116_cov_2.283695"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            430..>1116
                     /locus_tag="PLD67_09485"
     CDS             430..>1116
                     /locus_tag="PLD67_09485"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOT22795.1"
                     /translation="MPKYIFVTGGVVSSLGKGITAASLGRLLKSRGLKVTLQKFDPYL
                     NVDPGMMSPYQHGEVYVTDDGAETDLDLGHYERIVDIDLTQYSSITSGKIYWAVLNKE
                     RRGDYHGGTVQVIPHVTNEIKCMLKRIASVEDVDIVITEIGGTVGDIESQPFLEAIRQ
                     IRYEVGRENVLYIHVTLLPYLSMAGELKTKPTQHSVKELLSIGIQPDILVCRSEKEIP
                     EELKAKIALFC"
BASE COUNT          404 a          153 c          222 g          337 t
ORIGIN      
        1 aaatgtatca ttaaataatt taaccaaaat aaatcctcct ttaagttaat acagcattac
       61 gcattattct tttataaata tatatgccgt ttaaatttca atagtacaac ttataaataa
      121 aattttttca aaaagtatta aaaaggattg acctttttat ttatttaata taaaatgatt
      181 tagtaatttg atgacaattc tttaaattgc caaattaatt aaataattgc aaagagattc
      241 gaattaatag agactaaaaa aaaaacccct ttttgtctct tttttatgtt aacaaggtat
      301 gaagcaattg gggacattcc tcctgccggc tgattagatg ggaggtgtgt ccccatacct
      361 taaataaatt ggggtaggga atcttatatt aattaatatt attggaataa tagttatcag
      421 gaggaaaata tgccgaaata tatttttgta accgggggag ttgtttcttc attgggaaaa
      481 ggaataacag cagcttcatt gggaaggctt ttaaaaagca gaggacttaa agtaacattg
      541 cagaaatttg acccttattt aaacgtggac ccgggaatga tgagccctta tcagcatgga
      601 gaagtgtatg taactgacga cggagccgaa acagatttag atttaggaca ctatgaaaga
      661 atagttgata tagatttaac tcaatacagc agcatcacat cggggaaaat atactgggct
      721 gtattgaata aggaaagaag aggggattac catggaggaa cagtgcaggt tattcctcat
      781 gtaaccaatg aaatcaaatg tatgttaaaa agaattgcct cagttgagga tgttgatata
      841 gttataaccg aaatcggtgg aactgttgga gatattgaaa gccagccctt cttggaagct
      901 attcgtcaaa ttcgttacga ggtaggaaga gaaaatgtat tgtatataca tgttaccttg
      961 cttccttatt tgtcaatggc gggagaactg aaaacaaaac cgacacagca cagcgttaaa
     1021 gagcttttaa gcataggtat tcagccggat atattggtgt gcagaagcga aaaagaaata
     1081 cctgaagaac tgaaggcaaa aattgcatta ttttgt
//
LOCUS       DAOSOW010000465         1107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_133273_length_1107_cov_3.576046, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000465 DAOSOW010000000
VERSION     DAOSOW010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_133273_length_1107_cov_3.576046"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLD67_09490"
     CDS             <1..397
                     /locus_tag="PLD67_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HOT22796.1"
                     /translation="DSINIKTYVRLKKMNKSWDSFSKVFLHGGTIHEHIFIKNYDEPF
                     DKFGELLLAYDFKDVFLEGTEALVETGKYTKLEKLLDNKLMEHVKSAKYVPPYGIETL
                     AGYLIAKDNEIKIARIILAEKQQAYRRNL"
     gene            419..739
                     /locus_tag="PLD67_09495"
     CDS             419..739
                     /locus_tag="PLD67_09495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HOT22797.1"
                     /translation="MYKIGIIGDKQSVQGFKAVGLNVFDCNKAQEARRILHNIADDNY
                     AIIYVTESLYDEIKELIYEYNEERLPAIIPIPGMQGTLGIGIGNIKKAVEKAVGADIL
                     FSND"
     gene            772..>1107
                     /locus_tag="PLD67_09500"
     CDS             772..>1107
                     /locus_tag="PLD67_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOT22798.1"
                     /translation="MSQGRVVKISGPLVIADGMKEADMFDVVRVSEQGLIGEIIEMRG
                     EMASIQVYEETSGLAPGAPVVSTGAQLSVELGPGLIETIYDGIQRPLEEIRRIAGPNI
                     TRGISIPALN"
BASE COUNT          398 a          156 c          255 g          298 t
ORIGIN      
        1 tgattcaatt aatattaaga catatgtaag gcttaagaaa atgaataaat catgggattc
       61 tttcagcaaa gtatttttgc atggcggtac catacatgaa catatattta ttaaaaacta
      121 tgatgagcct tttgataaat ttggagaact tcttttagct tatgatttta aggatgtatt
      181 tctcgaggga actgaagcat tagttgaaac aggaaaatat acaaagcttg agaaactgtt
      241 agataacaag cttatggagc atgttaaatc tgccaagtat gttccgcctt acggaataga
      301 aactcttgca ggatatctca ttgctaagga taatgaaatt aaaattgcac gaataatttt
      361 agcggaaaaa cagcaggcat atcgccggaa cttataagag agaggttgag agagacttat
      421 gtataagata ggaattatag gagataagca aagtgttcaa gggttcaaag ctgttggctt
      481 gaatgtattc gactgcaata aagcacaaga agcaagacgt atactgcata atattgcaga
      541 tgataattat gcaataatat atgttacgga aagcttatat gacgaaataa aagaattaat
      601 atatgagtac aatgaagagc gtcttccggc aatcattcct ataccgggca tgcagggaac
      661 actaggcata gggattggca acataaagaa ggcagttgaa aaagcagttg gtgccgacat
      721 attgtttagt aacgattaac gtaaaaaaaa tgaaagcagg tgattagttt aatgagccaa
      781 ggtagagtag ttaaaatatc cggtccgctg gttattgcag atggcatgaa agaagccgat
      841 atgtttgacg ttgttcgtgt aagcgagcaa ggccttatag gggaaataat tgagatgcgc
      901 ggtgaaatgg catccataca ggtttatgaa gaaacatcag gcttagctcc gggggcaccg
      961 gttgtttcaa caggagcaca gctttctgtt gaattgggac ccggactaat tgaaacaata
     1021 tatgacggaa tacaaaggcc tttggaagaa ataagaagaa tagcaggacc taatattacg
     1081 agaggaataa gtattcctgc tcttaac
//
LOCUS       DAOSOW010000466         1102 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_134216_length_1102_cov_3.298949, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000466 DAOSOW010000000
VERSION     DAOSOW010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134216_length_1102_cov_3.298949"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..522
                     /locus_tag="PLD67_09505"
     CDS             157..522
                     /locus_tag="PLD67_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOT22799.1"
                     /translation="MKTLQEYLCLPYKMEILPDTEEGGYVVSFPELPGCLTCAETMEE
                     AIRNAEDAKKAWMEAAMEEHLTIPEPSRPEDYSGQFKLRIPKSLHQQLAIHAKQEGIS
                     MNQYCYLLTKYDTAAHHSE"
BASE COUNT          321 a          244 c          230 g          307 t
ORIGIN      
        1 cagcccagcg gtgtctccgt taatttctac cgtaatccct ttgatacggt ctgccataga
       61 aattcacctg cctttctaaa aaacaacttg acaccatatg cggcaccata ttataataaa
      121 gaaaaagaag tcattgagaa tcaggaggga tgatgaatga aaaccttaca agaatatttg
      181 tgtctgccct ataaaatgga gattctgcct gatacagaag agggcggata tgtggtttcg
      241 tttccggaac tgcccggctg cctgacttgt gcagaaacaa tggaagaagc catccgcaat
      301 gcggaagatg caaaaaaagc atggatggag gcagcgatgg aagaacacct gaccatccct
      361 gagccaagcc gcccggagga ttattccggt caattcaagc tccggattcc gaagtcattg
      421 catcaacagt tagcgattca cgcaaagcag gaaggcatca gcatgaatca gtactgttat
      481 ctcctaacca aatatgatac agctgcacac cattccgaat gaaaagaccc gtcaaatacc
      541 tgacgggtct ttttctgttt agaacttgtc aaagtccgct tgtgttgcct tgtaggcata
      601 tttcaagtca tcgttttctc gttccgtgaa catatcgtct accattccga ttgtcagcag
      661 ctccaaatcg ggcagagaaa gcccaatctg cacacaacgc agaagaaata agggtgtcgt
      721 catttcccgg tcaattgggc gatgtttttt ttagcctgta cctgaatccg caaaacttaa
      781 agtgcttcag actcaaaaat attcctattt tacgttgatt tcataagttt ttaagctgtt
      841 ttttatttca cccaatgggt gaagaaattt taaggcttta aaaggtcgga actgctcgta
      901 atatagccat attctccact ttatgtaagt atgaactctg cggatttggg attccatctg
      961 cagatcgtta tgattctcgt cttggacgtc attatcatat ttactgtata agctgtggca
     1021 aggttttcga tgctccatta gactatcacc tgaatccgca gacttgaaat tatgccacta
     1081 gccaaagccg cgaaaatatg gt
//
LOCUS       DAOSOW010000467         1088 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_136891_length_1088_cov_3.164569, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000467 DAOSOW010000000
VERSION     DAOSOW010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_136891_length_1088_cov_3.164569"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1088)
                     /locus_tag="PLD67_09510"
     CDS             complement(<1..>1088)
                     /locus_tag="PLD67_09510"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012290564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOT22800.1"
                     /translation="LIFEDAAVVTGFSLGASSIALFARVGGGIYTKAADVGADLVGKV
                     EAGIPEDDPRNPAVIADNVGDNVGDVAGMGADLFESYVGAIVSAITLGVLAYQGKNGV
                     SFALIVSAVGIIASILGTLFVKGDKNPQKALNMGTYASSAIVIAASFFLSKSILGSYQ
                     PFGSIIFGVFVGLIIAKITEIYTSDEYKYVKEIASQSETGTATTIISGLSVGMRSTAF
                     PIIVLAIGIIGAYAIAGYTSNGVDPVKGLFGISLAAVGMLSTTGMTIAVDAYGPISDN
                     AGGIAEMCELPHEVREITDRLDSVGNTTAAIGKGFAIGSAALTALALFASYTQAVGIT
                     IISLTEPKVIAGLFLGGMLPFLFSAITM"
BASE COUNT          322 a          242 c          184 g          340 t
ORIGIN      
        1 ctcatagtta ttgctgagaa taaaaatgga agcattccgc ctaggaataa accggctatt
       61 acttttggct ctgttaagct tataattgta attccaactg cctgagtata tgatgcaaac
      121 aaagcaaggg ctgttaaagc agctgagcct attgcaaatc ctttgccgat tgctgctgtt
      181 gtgttgccta cagagtctaa tctatccgtt atttctctta cttcatgagg aagctcgcac
      241 atttcggcaa tacctcctgc attgtcggat attggtccat aggcatcaac tgctattgtc
      301 atgcctgttg ttgataacat acctactgct gccaatgata taccaaataa tcctttgaca
      361 ggatcaacac cgtttgaagt ataacctgca attgcatatg ctcctataat acctattgcc
      421 aaaacaatta ttggaaacgc tgtggatctc attccgacag acaaaccgct tataattgtt
      481 gttgctgtac ctgtttcgga ttgtgaagct atttctttta catatttata ttcatctgaa
      541 gtatatattt cagttatttt tgctataatt aatcctacaa atacaccgaa tataattgaa
      601 ccaaagggtt ggtaactgcc caatatagac ttgcttaaga aaaatgatgc agctattact
      661 attgcagaag aagcatatgt acccatattt aaagcttttt gagggttttt gtctcctttt
      721 acaaacaatg taccgagtat tgacgcaata atacctacag cggatacaat taaagcaaat
      781 gaaacgccat ttttaccttg gtatgccaga acacccagtg ttattgcaga aactattgct
      841 ccaacatatg attcaaataa gtctgcaccc attcctgcta cgtcacctac attatcgcca
      901 acattatccg ctataacagc agggtttctc gggtcatctt cgggaattcc tgcttcaacc
      961 ttaccaacca ggtctgctcc aacgtctgca gcttttgtat aaatacctcc gcccacacgt
     1021 gcaaataatg ctattgatga agcgcctagg ctgaatcccg taactacagc tgcatcttca
     1081 aaaattaa
//
LOCUS       DAOSOW010000468         1024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_150785_length_1024_cov_2.561404, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000468 DAOSOW010000000
VERSION     DAOSOW010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_150785_length_1024_cov_2.561404"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(256..>1024)
                     /locus_tag="PLD67_09515"
     CDS             complement(256..>1024)
                     /locus_tag="PLD67_09515"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOT22801.1"
                     /translation="LPMTVEKTALKIVERVKRKCENNPLEYIKINISFGVATKTFMDE
                     DINKIYKKAEDRMYFNKLKESKEAKLSMIEFLKKRLEKITYETKSHYDRLKELTLMMA
                     EALNLTEEEKEELRLLCEFHDIGKIGVPKNILQKGSRLNSEEWEDVKRHSEIGYYIAK
                     EIKNASAIDELILTHHERWDGTGYPGLLKNNEIPLVVRIFALADAYDMMVNDRPFQTR
                     MTKEAALEEIKQQSGRQFDPNLAEIFIRLMEEEAQIV"
BASE COUNT          246 a          215 c          159 g          404 t
ORIGIN      
        1 agtaatgtac cccttcggat tgctttttat tcctgttttt ggcagtttgc cgatataatt
       61 gttgtaagct tcaaaaatgt tgcttgaatt atatatacct caatttttta tttataaaca
      121 tttattaatt atattaaact cttttactat agctaaaaaa tggtctcccg ttattcagac
      181 ggcactttca gcattaggat atttaaaaag aaatccttcg cttacgctca ggatgtcaaa
      241 aagggggttg ttttgctaga ctatctgcgc ttcttcttcc attagtctta taaaaatttc
      301 cgcaagattc gggtcgaatt gtcttcccga ttgttgtttg atttcctcca aggctgcttc
      361 ttttgtcatt ctcgtttgga aaggtctgtc attaaccatc atgtcataag catctgccaa
      421 agcaaatatt cttactacca aaggtatttc gttatttttc aacaaacccg gatatcccgt
      481 gccgtcccac cgctcatgat gcgtgagaat caattcgtca atagccgatg cattttttat
      541 ttcttttgca atatagtaac cgatttcgct gtgccttttt acatcttccc attcttcgct
      601 gttcaacctg cttccctttt gaaggatatt ctttggaaca cctattttcc ctatgtcgtg
      661 gaattcgcaa agaagtctca gttcttcctt ttcttcttca gtgagattta aagcctctgc
      721 catcatcaaa gtaagctctt tgagcctgtc gtaatgggat ttggtttcgt atgtgatttt
      781 ttcaagcctt ttcttcagga attctatcat ggaaagctta gcttccttgc tctctttcag
      841 cttattaaaa tacatcctgt cttctgcttt tttgtatatt ttgtttatat cttcgtccat
      901 gaatgtcttg gttgcaactc caaagcttat attaatttta atgtattcca aaggattgtt
      961 ttcgcacttc ctctttactc tctcgacaat tttcaaagca gttttttcca ctgtcatagg
     1021 caat
//
LOCUS       DAOSOW010000469         1016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_152635_length_1016_cov_3.259105, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000469 DAOSOW010000000
VERSION     DAOSOW010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_152635_length_1016_cov_3.259105"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLD67_09520"
     CDS             complement(<1..246)
                     /locus_tag="PLD67_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006314504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOT22802.1"
                     /translation="MPQVKIGVLISGSGTNLQSLIDNIETGNIKGKITVVISNRKDAY
                     GLNRARQKNIDALYIRQKDYESFENFNDAIIDELKKHN"
     gene            complement(234..>1016)
                     /gene="purM"
                     /locus_tag="PLD67_09525"
     CDS             complement(234..>1016)
                     /gene="purM"
                     /locus_tag="PLD67_09525"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOT22803.1"
                     /translation="VAMCVNDVICQGAKPLFFLDYIATGKLEPKKAASIVKGVSEGCI
                     KAGCALIGGETAEMPGLYDVGEYDIAGFCVGIVDKKKIIDGSKIIEGDILIGIPSSGI
                     HSNGYSLVRKVFFSHKNYDITDYVEELGKTLGEELLTPTRIYAKLVLDLISKFEIKGL
                     SNITGGGFYENIPRMFPQGITADIDTKNINVLPIFKLLQKKGNIDIDEMYGTFNMGIG
                     IVLTVAKDDADDILSYLKSVGETPVIIGRTVKREGGLKLCHR"
BASE COUNT          279 a          225 c          144 g          368 t
ORIGIN      
        1 attatgtttt tttaactcat caataatagc atcattgaaa ttttcaaagc tttcataatc
       61 cttttgcctt atatataggg catcgatgtt tttttgcctt gctctgttaa gtccataagc
      121 atcttttctg tttgaaatga caacggtaat tttacccttt atatttcctg tctcgatatt
      181 atcaatcaag gactgcagat tagttcctga gcctgaaatt aatacaccga tttttacctg
      241 tggcataatt taagcccgcc ttcccttttt actgttcttc ctattattac aggggtttca
      301 ccgacacttt tcaggtagct caaaatatcg tccgcatcat cttttgcaac agtcaatact
      361 atgccgatac ccatgttaaa tgtaccatac atttcatcaa tgtcaatgtt cccttttttt
      421 tgcaacagct taaatatggg aagaacattg atatttttag tatctatatc tgctgtaatg
      481 ccttgaggaa acatccttgg aatattttca taaaatcccc cgcctgtaat attagataaa
      541 ccctttattt caaatttaga tatgaggtca agtaccagct tggcataaat tcttgtcgga
      601 gttaataact cttctcccaa ggtttttcca agctcctcta catagtctgt aatatcatag
      661 tttttgtgac tgaaaaaaac ttttcgcact aaggagtatc cgttgctgtg aattccgctt
      721 gaaggaatac ctattaaaat atccccttct attatttttg agccgtcgat tattttcttt
      781 ttatcaacaa ttccaacaca aaatcccgca atatcatatt ctcccacgtc gtaaagtccc
      841 ggcatttcgg cagtttctcc tccaattaag gcacagcctg ccttaatgca gccctccgat
      901 acacctttaa caatgcttgc tgcttttttt ggctctagtt ttcctgttgc tatataatcc
      961 aggaaaaata aaggttttgc accctggcat ataacatcat ttacacacat ggctac
//
LOCUS       DAOSOW010000470         1014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_153182_length_1014_cov_2.582899, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000470 DAOSOW010000000
VERSION     DAOSOW010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153182_length_1014_cov_2.582899"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLD67_09530"
     CDS             <1..256
                     /locus_tag="PLD67_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22804.1"
                     /translation="DVDGNIVVGQIGTIPVVDFDEHNVLRVVGFVSFTITNLEGNNPN
                     QKKISILTSSYINDYITGGDAETGIVLNDYGVRAAKLVDY"
     gene            283..>1014
                     /gene="cpaB"
                     /locus_tag="PLD67_09535"
     CDS             283..>1014
                     /gene="cpaB"
                     /locus_tag="PLD67_09535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HOT22805.1"
                     /translation="MKKIKFLALLAAVVAAVLLYKFLNTLDEKASVEVVKTGVLTAVV
                     NIPANTPITNDMIAIKQLPVEAVHAEAVVNEVAAIGKFSKYEILAGEQILYTKLISPE
                     GGGGDGTLAYEIKPGMRAISIGVGNLSGLVGMIKPHNTVDIIAQFQKTEKQADGEEKT
                     IDYTTMIAENIKVLAVDDVISQEGKKINPETGTAYTSVTLQVTAHQAMELNMSEYKGQ
                     LRLILRSPLDEDMVNPPTIKFDDIII"
BASE COUNT          360 a          156 c          221 g          277 t
ORIGIN      
        1 tgatgttgac ggtaatatag tagttggtca gattggcaca attcccgtgg ttgattttga
       61 cgaacataat gtattgagag tagtaggctt tgtttcattt acaattacca atcttgaagg
      121 taataaccca aatcaaaaaa aaataagcat attgacatca tcctatataa atgattatat
      181 taccggcggt gatgcagaga cagggattgt attgaatgat tatggagtaa gagctgcaaa
      241 attggttgat tattaacaaa tacataaatt tggagtgagc ttatgaaaaa gataaaattt
      301 ttagcattac ttgcagcagt tgtagctgct gtcctcttat ataaatttct gaatacgctt
      361 gatgaaaaag caagcgttga ggtagtaaag acaggagtgt tgacagcagt tgtcaatatt
      421 ccggcaaata caccgataac aaatgatatg attgctataa aacagcttcc cgtcgaggct
      481 gtacatgcag aggcagtcgt taatgaggta gcagctatag gaaagttttc aaaatatgaa
      541 attctggcag gagaacaaat attatacact aagctgattt caccggaagg cggcggggga
      601 gatggaacgc ttgcatatga aataaaaccc ggtatgcgtg caatatcaat aggcgtaggt
      661 aatttatccg gacttgtcgg aatgattaag cctcataata cggtagatat tattgctcag
      721 tttcaaaaaa cggaaaagca agctgacgga gaggaaaaaa caatagatta tacaactatg
      781 atagcagaaa acatcaaggt tttagccgtt gacgacgtaa tatctcaaga aggcaaaaaa
      841 atcaatcctg aaacgggtac tgcttataca tccgtaacat tgcaagttac ggcacatcag
      901 gctatggagc tgaatatgtc cgaatacaag ggacagctta gattaatatt aaggtccccg
      961 ttggatgaag atatggttaa tcctccaaca ataaaattcg acgatattat aatt
//
LOCUS       DAOSOW010000471         1012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_153638_length_1012_cov_3.507837, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000471 DAOSOW010000000
VERSION     DAOSOW010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_153638_length_1012_cov_3.507837"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLD67_09540"
     CDS             complement(<1..302)
                     /locus_tag="PLD67_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOT22806.1"
                     /translation="MYVEKIVTTGICQGKLFLFIMELLKEDYSFKKALYLGAGDSFAI
                     HSIEVFNDAVYMTDSFNNKIYKFDSVNNFFETNVGRDPRHMCTDKDNIYAANFESD"
     gene            complement(425..>1012)
                     /locus_tag="PLD67_09545"
     CDS             complement(425..>1012)
                     /locus_tag="PLD67_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOT22807.1"
                     /translation="IELNPTLRLRSLTEGMTICVPRVPERPPCVNGAYYTIREGDSFY
                     NIAQRYGIPLNLLLEANPEANPYDLKVGQEICIPDVPPGCPFGTVVTIQEGTRLSDIL
                     ISYNLSLNELRDANVNFNPNIIVPGTELCIPQETFMQCPEGTTTEYVIQAGDSLSTVA
                     IANNITPSELLIANPHLRPANFLIVGTRVCIPTAR"
BASE COUNT          306 a          201 c          159 g          346 t
ORIGIN      
        1 ttatccgatt caaaatttgc tgcatatata ttatctttgt ccgtgcacat atgccgcggg
       61 tctcttccca cattggtttc aaaaaaatta tttacgctgt cgaatttgta aattttgtta
      121 ttaaaggaat ctgtcatata taccgcatca ttaaaaactt ctatgctgtg gattgcaaag
      181 ctgtcgcctg ctcccaaata taaagctttt ttaaaagaat aatcctcttt taaaagctcc
      241 attataaaca aaaataattt tccctgacat atgcctgttg taacgatttt ttcaacatac
      301 actgcaatca cctccatata gtttatgaat cacaacaaaa gaagtgaata tttttgataa
      361 attcctcaaa tgatattaga ctaattaaaa cacagcagtt ttcactgctg tgctgatttt
      421 catattatcg tgctgtcggt atacacactc ttgttcctac aatcagaaag tttgccggtc
      481 tcaaatgagg atttgcaata agtaattctg aaggtgtaat attatttgca attgccactg
      541 tagaaagact gtctcccgcc tgtattacgt attctgtggt tgttccttca ggacactgca
      601 taaatgtttc ctgaggtatg catagttcgg ttccgggtac aataatatta ggattgaaat
      661 taacatttgc atctcttaat tcatttaagc ttaaattgta agatatgaga atatcgctta
      721 accttgttcc ttcttgtata gtcacaaccg taccgaaagg acaccccgga ggaacatcag
      781 gtatgcatat ttcctgacca actttcaaat catagggatt tgcttccgga ttggcttcta
      841 atagcaaatt caaaggaatg ccatatcttt gcgcaatatt ataaaaacta tctccttccc
      901 ttatggtata gtaggctcca ttaacacatg gcggtctttc aggaaccctt ggcacacata
      961 tagtcattcc ttccgttaat gaccttaatc ttaatgtagg attcaattca at
//
LOCUS       DAOSOW010000472         1006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Sedimentibacter sp. isolate AMR_MDS_2335
            NODE_155234_length_1006_cov_2.545741, whole genome shotgun
            sequence.
ACCESSION   DAOSOW010000472 DAOSOW010000000
VERSION     DAOSOW010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864402
            Sequence Read Archive: SRR4236788
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sedimentibacter sp. (wastewater metagenome)
  ORGANISM  Sedimentibacter sp.
            Bacteria; Bacillati; Bacillota; Tissierellia; Sedimentibacter.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 03:48:54
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,911
            CDSs (total)                      :: 1,889
            Genes (coding)                    :: 1,870
            CDSs (with protein)               :: 1,870
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 19
            CDSs (without protein)            :: 19
            Pseudo Genes (ambiguous residues) :: 0 of 19
            Pseudo Genes (frameshifted)       :: 7 of 19
            Pseudo Genes (incomplete)         :: 9 of 19
            Pseudo Genes (internal stop)      :: 4 of 19
            Pseudo Genes (multiple problems)  :: 1 of 19
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Sedimentibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_155234_length_1006_cov_2.545741"
                     /isolate="AMR_MDS_2335"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1960295"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PLD67_09550"
     CDS             <1..756
                     /locus_tag="PLD67_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HOT22808.1"
                     /translation="NMSRGFYTLASGMITQQRKIDVSSNNVANLNTAGYKKEQAITSN
                     FGSLLINKYKQNGIKEEAEPINNISLIRAVTENNTIHSQGVLEETGNITDFAIIGAGF
                     FAIDNEGQILYTRNGSFNIDEEGYLFLEGIGRVQGEFGDIYVEGDKFEFLEDGSIIIE
                     GEIADKIAVFDFPDYADLNKYGEGMFISDAEPDLSEYPIIRNKTIERSNVEITEELTN
                     IISSQRALQTAAQALKIYDSINDKAVNEIGKVQ"
     gene            766..>1006
                     /locus_tag="PLD67_09555"
     CDS             766..>1006
                     /locus_tag="PLD67_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HOT22809.1"
                     /translation="MIKAYYTATNGAISNKNYLDVISNNMANIQTEGFKKSKPEFSDL
                     IYTNMRGTKGADTELKSGSGSKISKVDVVFSQGTPY"
BASE COUNT          395 a          140 c          210 g          261 t
ORIGIN      
        1 aatatgtcaa gaggatttta tacattagct tcaggtatga ttactcagca aagaaaaatc
       61 gatgtatcaa gcaataatgt agcaaactta aatacggcag gttataaaaa ggaacaagcc
      121 ataacttcaa atttcggcag cttactgata aataaataca aacaaaatgg aataaaagaa
      181 gaagcagagc caatcaacaa tatttctctt attcgagccg taacggaaaa caataccata
      241 cattcgcagg gggttttgga ggaaacagga aatattacgg attttgcaat tattggagcg
      301 ggattctttg caattgataa tgaggggcaa attttataca cccgtaacgg aagctttaac
      361 atagatgaag aaggatattt gtttcttgaa ggtattggaa gagtacaggg ggaatttgga
      421 gatatatatg tagaaggtga taaatttgaa tttctggaag acggaagtat aataatcgaa
      481 ggtgaaattg cggacaaaat tgctgttttt gacttccctg attacgctga tttaaacaaa
      541 tatggcgaag gaatgtttat ttcggatgca gagccggact tatcagaata tccaatcata
      601 agaaataaaa caattgaaag gtccaatgtt gaaattacgg aagaattaac aaatataatt
      661 tcatcacaaa gggctttgca aacagcagct caagcgctaa aaatatatga ttcgataaat
      721 gacaaagcag tcaatgaaat aggaaaggta caataggagg cctatatgat taaagcatat
      781 tatacggcaa caaacggagc aataagcaat aaaaattatt tggatgttat atcaaacaat
      841 atggcaaaca tacagacaga agggtttaag aaatcaaagc ctgaattttc cgatttaatc
      901 tataccaata tgagagggac taaaggagcc gacacggaat taaaatcagg aagcgggtct
      961 aaaatcagta aggttgatgt tgttttcagt cagggaactc catatc
//
